Sgr017818 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr017818 vs. NCBI nr
Match: KAG7024466.1 (AFG1-like ATPase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 826.2 bits (2133), Expect = 1.6e-235
Identity = 432/529 (81.66%), Postives = 444/529 (83.93%), Query Frame = 0

Query: 69  VKLANWKQNRENERRRLLTEEAESKQQGDVWTSVNKRRSKLVETLMFRKKSENIEPGVGK 128
           VKLANWKQ+RENERRRLL EEAESKQQGDVWTSVNKRRSKL ETLMFRK SENIEPGVGK
Sbjct: 164 VKLANWKQSRENERRRLLMEEAESKQQGDVWTSVNKRRSKLAETLMFRKSSENIEPGVGK 223

Query: 129 WVSYLNRERKLDSLVGRCPSAPHAPTGLYIYGNVGSGKTMLMDMFYNATEGVVKHRRR-- 188
           WVSYLNRERKLDSLVGRCPSAPHAP GLYIYGNVGSGKTMLMDMFYNATEG+VKHR+R  
Sbjct: 224 WVSYLNRERKLDSLVGRCPSAPHAPRGLYIYGNVGSGKTMLMDMFYNATEGIVKHRQRYH 283

Query: 189 ---IGIGLQFYFIFWQAMLKINEDMHKIWKNQVREKPSQSSISSWIMNLPFDTKVKEWLA 248
              IGIG+Q Y I WQAMLKINEDMHKIWKNQVREK SQSSISSWIMNLPFDTKVKEW+A
Sbjct: 284 FHEIGIGIQVYSIIWQAMLKINEDMHKIWKNQVREKSSQSSISSWIMNLPFDTKVKEWVA 343

Query: 249 AEEKYKQE------------------QADPVGASILCFDEIQTVDVFAIVALSGIVSRLL 308
           AEEKYKQE                  QA  VGASILCFDEIQTVDVFAIVALSGIVSRLL
Sbjct: 344 AEEKYKQEVQMKNILPAVADKFLVDQQAGQVGASILCFDEIQTVDVFAIVALSGIVSRLL 403

Query: 309 STGTVLVATSNRAPKDLNQDGMQKDIFQKFVAKLEEHCKFVLM----------------E 368
           STGTVLVATSNRAPKDLNQDGMQKDIFQKFV KLEEHC FVL+                +
Sbjct: 404 STGTVLVATSNRAPKDLNQDGMQKDIFQKFVTKLEEHCAFVLIGSEIDYRRFIAQRSFDQ 463

Query: 369 VHYFWPLDCTSVGKFEKMWVEITRQLGGQITSETIPVMFGRKLEVSESCNGVARFAFDFL 428
           VHYFWP DCTSVGKFEKMWVEIT QLGGQITSETIPVMFGRKLEVSESCNGVARFAFDFL
Sbjct: 464 VHYFWPSDCTSVGKFEKMWVEITSQLGGQITSETIPVMFGRKLEVSESCNGVARFAFDFL 523

Query: 429 CGQPVGAADYIALAENYHTVFISNIPMMSMCIRDKARRFIALIDELYNHHCCLFCLAATS 488
           CGQPVGAADYIALA+NYHTVFIS+IP+MSM IRDKARRFI LIDELYNHHCCLFCLAATS
Sbjct: 524 CGQPVGAADYIALAKNYHTVFISDIPVMSMRIRDKARRFITLIDELYNHHCCLFCLAATS 583

Query: 489 IEDLFQGTEEGALFDLE------RGRRGKASSKCPSGRQCRLCRAPTAIVSMLSGQEEMF 548
           IEDLFQGTEEGALFDLE          GK               APTAIVSMLSGQEEMF
Sbjct: 584 IEDLFQGTEEGALFDLESFQFETEAEGGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMF 643

Query: 549 AFRRAVSRLIEMQTPLYLEGVRSLHPYFQRREQESLIDRATRFQSQLSS 553
           AFRRAVSRLIEMQTPLYLEGVR+LHPYFQRRE ESL+D A RFQ Q SS
Sbjct: 644 AFRRAVSRLIEMQTPLYLEGVRNLHPYFQRREPESLVDSAARFQFQQSS 692

BLAST of Sgr017818 vs. NCBI nr
Match: XP_022135602.1 (AFG1-like ATPase isoform X1 [Momordica charantia])

HSP 1 Score: 819.3 bits (2115), Expect = 2.0e-233
Identity = 428/524 (81.68%), Postives = 443/524 (84.54%), Query Frame = 0

Query: 69  VKLANWKQNRENERRRLLTEEAESKQQGDVWTSVNKRRSKLVETLMFRKKSENIEPGVGK 128
           VKLANWKQNRENERR+LL EEAESKQQGDVWTSVNKR+SKLVETLMFR KSENIEPGVGK
Sbjct: 125 VKLANWKQNRENERRKLLMEEAESKQQGDVWTSVNKRQSKLVETLMFRNKSENIEPGVGK 184

Query: 129 WVSYLNRERKLDSLVGRCPSAPHAPTGLYIYGNVGSGKTMLMDMFYNATEGVVKHRRRIG 188
           WVSYLNRERKLDSLVGRCPSAPHAP GLYIYGNVGSGKTMLMDMFYNAT+G+VKHRRR  
Sbjct: 185 WVSYLNRERKLDSLVGRCPSAPHAPRGLYIYGNVGSGKTMLMDMFYNATKGIVKHRRR-- 244

Query: 189 IGLQFYFIFWQAMLKINEDMHKIWKNQVREKPSQSSISSWIMNLPFDTKVKEWLAAEEKY 248
                 + F +AMLKINEDMH+IWKNQVREK SQSSISSWIMNLPFDTKVKEWLAAEEKY
Sbjct: 245 ------YHFHEAMLKINEDMHQIWKNQVREKSSQSSISSWIMNLPFDTKVKEWLAAEEKY 304

Query: 249 KQE------------------QADPVGASILCFDEIQTVDVFAIVALSGIVSRLLSTGTV 308
           KQE                  QAD VGASILCFDEIQTVDVFAIVALSGIVSRLLSTGTV
Sbjct: 305 KQEVQMKNILPAVADKFLVDQQADQVGASILCFDEIQTVDVFAIVALSGIVSRLLSTGTV 364

Query: 309 LVATSNRAPKDLNQDGMQKDIFQKFVAKLEEHCKFVLM----------------EVHYFW 368
           LVATSNRAPKDLNQDGMQKDIFQKFVAKLEEHC+FVL+                +VHYFW
Sbjct: 365 LVATSNRAPKDLNQDGMQKDIFQKFVAKLEEHCEFVLIGSEIDYRRFIAQRSFDQVHYFW 424

Query: 369 PLDCTSVGKFEKMWVEITRQLGGQITSETIPVMFGRKLEVSESCNGVARFAFDFLCGQPV 428
           PL+ TSVGKFEKMW E+TRQLGGQITSETI VMFGRKLEVSESCNGVARFAFDFLCGQPV
Sbjct: 425 PLELTSVGKFEKMWAEMTRQLGGQITSETISVMFGRKLEVSESCNGVARFAFDFLCGQPV 484

Query: 429 GAADYIALAENYHTVFISNIPMMSMCIRDKARRFIALIDELYNHHCCLFCLAATSIEDLF 488
           GAADYIALA+NYHTVFISNIPMMSM IRDKARRFI LIDELYNHHCCLFCLAATSIEDLF
Sbjct: 485 GAADYIALAQNYHTVFISNIPMMSMRIRDKARRFITLIDELYNHHCCLFCLAATSIEDLF 544

Query: 489 QGTEEGALFDLE------RGRRGKASSKCPSGRQCRLCRAPTAIVSMLSGQEEMFAFRRA 548
           QGTEEG LFDLE          GK               APTAIVSMLSGQEEMFAFRRA
Sbjct: 545 QGTEEGTLFDLESFQFETEAEGGKLRRNVLEEGNVGSVGAPTAIVSMLSGQEEMFAFRRA 604

Query: 549 VSRLIEMQTPLYLEGVRSLHPYFQRREQESLIDRATRFQSQLSS 553
           VSRLIEMQTPLYLEGVRSLHPYFQRREQESL+DRAT+F SQLSS
Sbjct: 605 VSRLIEMQTPLYLEGVRSLHPYFQRREQESLVDRATKFHSQLSS 640

BLAST of Sgr017818 vs. NCBI nr
Match: XP_022936535.1 (AFG1-like ATPase [Cucurbita moschata] >XP_022936536.1 AFG1-like ATPase [Cucurbita moschata])

HSP 1 Score: 808.1 bits (2086), Expect = 4.6e-230
Identity = 424/524 (80.92%), Postives = 436/524 (83.21%), Query Frame = 0

Query: 69  VKLANWKQNRENERRRLLTEEAESKQQGDVWTSVNKRRSKLVETLMFRKKSENIEPGVGK 128
           VKLANWKQ+RENERRRLL EEAESKQQGDVWTSVNKRRSKL ETLMFRK SENIEPGVGK
Sbjct: 90  VKLANWKQSRENERRRLLMEEAESKQQGDVWTSVNKRRSKLAETLMFRKSSENIEPGVGK 149

Query: 129 WVSYLNRERKLDSLVGRCPSAPHAPTGLYIYGNVGSGKTMLMDMFYNATEGVVKHRRRIG 188
           WVSYLNRERKLDSLVGRCPSAPHAP GLYIYGNVGSGKTMLMDMFYNATEG+VKHR+R  
Sbjct: 150 WVSYLNRERKLDSLVGRCPSAPHAPRGLYIYGNVGSGKTMLMDMFYNATEGIVKHRQR-- 209

Query: 189 IGLQFYFIFWQAMLKINEDMHKIWKNQVREKPSQSSISSWIMNLPFDTKVKEWLAAEEKY 248
                 + F +AMLKINEDMHKIWKNQVREK SQSSISSWIMNLPFDTKVKEW+AAEEKY
Sbjct: 210 ------YHFHEAMLKINEDMHKIWKNQVREKSSQSSISSWIMNLPFDTKVKEWVAAEEKY 269

Query: 249 KQE------------------QADPVGASILCFDEIQTVDVFAIVALSGIVSRLLSTGTV 308
           KQE                  QA  VGASILCFDEIQTVDVFAIVALSGIVSRLLSTGTV
Sbjct: 270 KQEVQMKNILPAVADKFLVDQQAGQVGASILCFDEIQTVDVFAIVALSGIVSRLLSTGTV 329

Query: 309 LVATSNRAPKDLNQDGMQKDIFQKFVAKLEEHCKFVLM----------------EVHYFW 368
           LVATSNRAPKDLNQDGMQKDIFQKFV KLEEHC FV +                +VHYFW
Sbjct: 330 LVATSNRAPKDLNQDGMQKDIFQKFVTKLEEHCAFVFIGSEIDYRRFIAQRSFDQVHYFW 389

Query: 369 PLDCTSVGKFEKMWVEITRQLGGQITSETIPVMFGRKLEVSESCNGVARFAFDFLCGQPV 428
           P DCTSVGKFEKMWVEIT QLGGQITSETIPVMFGRKLEVSESCNGVARFAFDFLCGQPV
Sbjct: 390 PSDCTSVGKFEKMWVEITSQLGGQITSETIPVMFGRKLEVSESCNGVARFAFDFLCGQPV 449

Query: 429 GAADYIALAENYHTVFISNIPMMSMCIRDKARRFIALIDELYNHHCCLFCLAATSIEDLF 488
           GAADYIALA+NYHTVFIS+IPMMSM IRDKARRFI LIDELYNHHCCLFCLAATSIEDLF
Sbjct: 450 GAADYIALAKNYHTVFISDIPMMSMRIRDKARRFITLIDELYNHHCCLFCLAATSIEDLF 509

Query: 489 QGTEEGALFDLE------RGRRGKASSKCPSGRQCRLCRAPTAIVSMLSGQEEMFAFRRA 548
           QGTEEGALFDLE          GK               APTAIVSMLSGQEEMFAFRRA
Sbjct: 510 QGTEEGALFDLESFQFETEAEGGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRA 569

Query: 549 VSRLIEMQTPLYLEGVRSLHPYFQRREQESLIDRATRFQSQLSS 553
           VSRLIEMQTPLYLEGVR+LHPYFQRRE ESL+D A RFQ Q SS
Sbjct: 570 VSRLIEMQTPLYLEGVRNLHPYFQRREPESLVDSAARFQFQQSS 605

BLAST of Sgr017818 vs. NCBI nr
Match: KAG6591578.1 (AFG1-like ATPase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 805.4 bits (2079), Expect = 3.0e-229
Identity = 423/524 (80.73%), Postives = 437/524 (83.40%), Query Frame = 0

Query: 69  VKLANWKQNRENERRRLLTEEAESKQQGDVWTSVNKRRSKLVETLMFRKKSENIEPGVGK 128
           VKLANWKQ+RENERRRLL EEAESKQQGDVWTSVNKRRSKL ETLMFRK SENIEPGVGK
Sbjct: 149 VKLANWKQSRENERRRLLMEEAESKQQGDVWTSVNKRRSKLAETLMFRKSSENIEPGVGK 208

Query: 129 WVSYLNRERKLDSLVGRCPSAPHAPTGLYIYGNVGSGKTMLMDMFYNATEGVVKHRRRIG 188
           WVSYLNRERKLDSLVGRCPSAP+AP GLYIYGNVGSGKTMLMDMFYNATEG+VKHR+R  
Sbjct: 209 WVSYLNRERKLDSLVGRCPSAPNAPRGLYIYGNVGSGKTMLMDMFYNATEGIVKHRQR-- 268

Query: 189 IGLQFYFIFWQAMLKINEDMHKIWKNQVREKPSQSSISSWIMNLPFDTKVKEWLAAEEKY 248
                 + F +AMLKINEDMHKIWKNQVREK SQSSISSWIMNLPFDTKVKEW+AAEEKY
Sbjct: 269 ------YHFHEAMLKINEDMHKIWKNQVREKSSQSSISSWIMNLPFDTKVKEWVAAEEKY 328

Query: 249 KQE------------------QADPVGASILCFDEIQTVDVFAIVALSGIVSRLLSTGTV 308
           KQE                  QA  VGASILCFDEIQTVDVFAIVALSGIVSRLLSTGTV
Sbjct: 329 KQEVQMKNILPAVADKFLVDQQAGQVGASILCFDEIQTVDVFAIVALSGIVSRLLSTGTV 388

Query: 309 LVATSNRAPKDLNQDGMQKDIFQKFVAKLEEHCKFVLM----------------EVHYFW 368
           LVATSNRAPKDLNQDGMQKDIFQKFV KLEEHC FVL+                +VHYFW
Sbjct: 389 LVATSNRAPKDLNQDGMQKDIFQKFVTKLEEHCAFVLIGSEIDYRRFIAQRSFDQVHYFW 448

Query: 369 PLDCTSVGKFEKMWVEITRQLGGQITSETIPVMFGRKLEVSESCNGVARFAFDFLCGQPV 428
           P DCTSVGKFEKMWVEIT QLGGQITSETIPVMFGRKLEVSESCNGVARFAFDFLCGQPV
Sbjct: 449 PSDCTSVGKFEKMWVEITSQLGGQITSETIPVMFGRKLEVSESCNGVARFAFDFLCGQPV 508

Query: 429 GAADYIALAENYHTVFISNIPMMSMCIRDKARRFIALIDELYNHHCCLFCLAATSIEDLF 488
           GAADYIALA+NYHTVFIS+IP+MSM IRDKARRFI LIDELYNHHCCLFCLAATSIEDLF
Sbjct: 509 GAADYIALAKNYHTVFISDIPVMSMRIRDKARRFITLIDELYNHHCCLFCLAATSIEDLF 568

Query: 489 QGTEEGALFDLE------RGRRGKASSKCPSGRQCRLCRAPTAIVSMLSGQEEMFAFRRA 548
           QGTEEGALFDLE          GK               APTAIVSMLSGQEEMFAFRRA
Sbjct: 569 QGTEEGALFDLESFQFETEAEGGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRA 628

Query: 549 VSRLIEMQTPLYLEGVRSLHPYFQRREQESLIDRATRFQSQLSS 553
           VSRLIEMQTPLYLEGVR+LHPYFQRRE ESL+D A RFQ Q SS
Sbjct: 629 VSRLIEMQTPLYLEGVRNLHPYFQRREPESLVDSAARFQFQQSS 664

BLAST of Sgr017818 vs. NCBI nr
Match: XP_023536384.1 (AFG1-like ATPase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 802.4 bits (2071), Expect = 2.5e-228
Identity = 421/524 (80.34%), Postives = 434/524 (82.82%), Query Frame = 0

Query: 69  VKLANWKQNRENERRRLLTEEAESKQQGDVWTSVNKRRSKLVETLMFRKKSENIEPGVGK 128
           VKLANWKQ+RENERRRLL EEAESKQQGDVWTSVN+RRSKL ETLMFRK SENIEPGVGK
Sbjct: 90  VKLANWKQSRENERRRLLMEEAESKQQGDVWTSVNRRRSKLAETLMFRKSSENIEPGVGK 149

Query: 129 WVSYLNRERKLDSLVGRCPSAPHAPTGLYIYGNVGSGKTMLMDMFYNATEGVVKHRRRIG 188
           WVSYLNRERKLDSLVGRCPSAPHAP GLYIYGNVGSGKTMLMDMFYNATEG+VKHR+R  
Sbjct: 150 WVSYLNRERKLDSLVGRCPSAPHAPRGLYIYGNVGSGKTMLMDMFYNATEGIVKHRQR-- 209

Query: 189 IGLQFYFIFWQAMLKINEDMHKIWKNQVREKPSQSSISSWIMNLPFDTKVKEWLAAEEKY 248
                 + F +AMLKINEDMH IWKNQVREK SQSSISSWIMNLPFDTKVKEW+AAEEKY
Sbjct: 210 ------YHFHEAMLKINEDMHNIWKNQVREKSSQSSISSWIMNLPFDTKVKEWVAAEEKY 269

Query: 249 KQE------------------QADPVGASILCFDEIQTVDVFAIVALSGIVSRLLSTGTV 308
           KQE                  QA  VGASILCFDEIQTVDVFAIVALSGIVSRLLSTGTV
Sbjct: 270 KQEVQMKNILPAVADKFLVDQQAGQVGASILCFDEIQTVDVFAIVALSGIVSRLLSTGTV 329

Query: 309 LVATSNRAPKDLNQDGMQKDIFQKFVAKLEEHCKFVLM----------------EVHYFW 368
           LVATSNRAPKDLNQDGMQKDIFQKFV KLEEHC FVL+                +VHYFW
Sbjct: 330 LVATSNRAPKDLNQDGMQKDIFQKFVTKLEEHCAFVLIGSEIDYRRFIAQRSFDQVHYFW 389

Query: 369 PLDCTSVGKFEKMWVEITRQLGGQITSETIPVMFGRKLEVSESCNGVARFAFDFLCGQPV 428
           P DCTSVGKFEKMWVEIT QLGGQITSETIPVMFGRKLEVSESCNGVARFAFDFLCGQPV
Sbjct: 390 PSDCTSVGKFEKMWVEITSQLGGQITSETIPVMFGRKLEVSESCNGVARFAFDFLCGQPV 449

Query: 429 GAADYIALAENYHTVFISNIPMMSMCIRDKARRFIALIDELYNHHCCLFCLAATSIEDLF 488
           GAADYIALA+NYHTVFIS+IPMMSM IRDKARRFI LIDELYNHHCCLFCLAATSIEDLF
Sbjct: 450 GAADYIALAKNYHTVFISDIPMMSMRIRDKARRFITLIDELYNHHCCLFCLAATSIEDLF 509

Query: 489 QGTEEGALFDLE------RGRRGKASSKCPSGRQCRLCRAPTAIVSMLSGQEEMFAFRRA 548
           QGTEEGALFDLE          GK               APTAIVSMLSGQEEMFAFRRA
Sbjct: 510 QGTEEGALFDLESFQFETEAEGGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRA 569

Query: 549 VSRLIEMQTPLYLEGVRSLHPYFQRREQESLIDRATRFQSQLSS 553
           VSRLIEMQTPLYLEGVR+LHPYFQRRE ESL+D A R   Q SS
Sbjct: 570 VSRLIEMQTPLYLEGVRNLHPYFQRREPESLVDSAARLHFQQSS 605

BLAST of Sgr017818 vs. ExPASy Swiss-Prot
Match: Q32PX9 (AFG1-like ATPase OS=Rattus norvegicus OX=10116 GN=Afg1l PE=2 SV=1)

HSP 1 Score: 192.6 bits (488), Expect = 1.2e-47
Identity = 134/390 (34.36%), Postives = 204/390 (52.31%), Query Frame = 0

Query: 153 PTGLYIYGNVGSGKTMLMDMFYNATEGVVKHRRRIGIGLQFYFIFWQAMLKINEDMHKIW 212
           P GLY+YG+VG+GKTM+MDMFY   E  VK +RR+         F   ML +++ +H++ 
Sbjct: 129 PKGLYVYGDVGTGKTMVMDMFYEYVE--VKSKRRVH--------FHGFMLDVHKRIHRLK 188

Query: 213 KNQVREKPSQSSISSWIMNLPFDTKVKEWLA-AEEKYKQEQADPVGASILCFDEIQTVDV 272
           ++  + K            + F  K  + +A   E+  QE      AS+LCFDE Q  D+
Sbjct: 189 QSLPKRK------------VGFMAKSYDPIAPIAEEISQE------ASLLCFDEFQVTDI 248

Query: 273 FAIVALSGIVSRLLSTGTVLVATSNRAPKDLNQDGMQKDIFQKFVAKLEEHCKFVLMEVH 332
              + L  +   L   G V+VATSNR P+DL ++G+Q+  F  F+A L+E+C  V ++  
Sbjct: 249 ADAMILKQLFENLFKNGVVVVATSNRPPEDLYKNGLQRANFVPFIAVLKEYCNTVQLDSG 308

Query: 333 Y-FWPLDCTSVGK-------------FEKMWVEITRQLGGQITSETIPVMFGRKLEVSES 392
             +   + T  GK              +K++ E+  Q    +TS  I  + GR+L ++++
Sbjct: 309 VDYRKRELTPAGKLYYLTSEADVGTVMDKLFDELA-QKQNDLTSPRILKVQGRELRLNKA 368

Query: 393 CNGVARFAFDFLCGQPVGAADYIALAENYHTVFISNIPMMSMCIRDKARRFIALIDELYN 452
           C  VA   F+ LC +P+GA+DY+ L++N+ TV I NIP  S+  R + RRFI LID  Y+
Sbjct: 369 CGTVADCTFEELCERPLGASDYLELSKNFDTVIIRNIPQFSLAKRTQVRRFITLIDNFYD 428

Query: 453 HHCCLFCLAATSIEDLFQGTEEGALFDLERGRRGKASSKCPSGRQCRLCRAPTAIVSMLS 512
               + C A+  I  LF    + +  D  R           S           A +SM +
Sbjct: 429 FKVRIICSASVPISSLFVYQHQDSESDQSRVLMDDLGLSQDS-----------AGLSMFT 478

Query: 513 GQEEMFAFRRAVSRLIEMQTPLY-LEGVRS 527
           G+EE+F+F+R +SRL EMQT  Y +EG RS
Sbjct: 489 GEEEIFSFQRTLSRLTEMQTEQYWIEGDRS 478

BLAST of Sgr017818 vs. ExPASy Swiss-Prot
Match: Q3V384 (AFG1-like ATPase OS=Mus musculus OX=10090 GN=Afg1l PE=1 SV=1)

HSP 1 Score: 188.3 bits (477), Expect = 2.3e-46
Identity = 130/390 (33.33%), Postives = 198/390 (50.77%), Query Frame = 0

Query: 153 PTGLYIYGNVGSGKTMLMDMFYNATEGVVKHRRRIGIGLQFYFIFWQAMLKINEDMHKIW 212
           P GLY+YG+VG+GKTM+MDMFY   E   K ++R+         F   ML ++  +H + 
Sbjct: 129 PKGLYVYGDVGTGKTMVMDMFYAYVE--TKRKKRVH--------FHGFMLDVHRRIHHLK 188

Query: 213 KNQVREKPSQSSISSWIMNLPFDTKVKEWLA-AEEKYKQEQADPVGASILCFDEIQTVDV 272
           ++  + K              F  K  + +A   E+  QE       S+LCFDE Q  D+
Sbjct: 189 QSLPKRKAG------------FMAKSYDPIAPIAEEISQE------TSLLCFDEFQVTDI 248

Query: 273 FAIVALSGIVSRLLSTGTVLVATSNRAPKDLNQDGMQKDIFQKFVAKLEEHCKFVLMEVH 332
              + L  +   L   G V+VATSNR P+DL ++G+Q+  F  F+A L+E+C  + ++  
Sbjct: 249 ADAMILKQLFENLFKNGVVVVATSNRPPEDLYKNGLQRANFVPFIAVLKEYCDTLQLDSG 308

Query: 333 --------------YFWPLDCTSVGKFEKMWVEITRQLGGQITSETIPVMFGRKLEVSES 392
                         Y+   +       +K++ E+  Q    +TS  I  + GR+L ++++
Sbjct: 309 VDYRKRELAPAGKLYYLTSEADVEAVVDKLFDELA-QKQNDLTSPRILKVQGRELRLNKA 368

Query: 393 CNGVARFAFDFLCGQPVGAADYIALAENYHTVFISNIPMMSMCIRDKARRFIALIDELYN 452
           C  VA   F+ LC +P+GA+DY+ L++N+ TV I NIP  S+  R +ARRFI LID  Y+
Sbjct: 369 CGSVADCTFEELCERPLGASDYLELSKNFDTVIIRNIPQFSLAKRTQARRFITLIDNFYD 428

Query: 453 HHCCLFCLAATSIEDLFQGTEEGALFDLERGRRGKASSKCPSGRQCRLCRAPTAIVSMLS 512
               + C A+  I  LF    + +  D  R           S           A +SM +
Sbjct: 429 FKVRIICSASAPISSLFLHQHQDSESDQSRILMDDLGLSQDS-----------AGLSMFT 478

Query: 513 GQEEMFAFRRAVSRLIEMQTPLY-LEGVRS 527
           G+EE+FAF+R +SRL EMQT  Y +EG RS
Sbjct: 489 GEEEIFAFQRTISRLTEMQTEQYWIEGDRS 478

BLAST of Sgr017818 vs. ExPASy Swiss-Prot
Match: Q8WV93 (AFG1-like ATPase OS=Homo sapiens OX=9606 GN=AFG1L PE=1 SV=2)

HSP 1 Score: 187.6 bits (475), Expect = 3.8e-46
Identity = 125/395 (31.65%), Postives = 202/395 (51.14%), Query Frame = 0

Query: 140 DSLVGRCPSAPHAPTGLYIYGNVGSGKTMLMDMFYNATEGVVKHRRRIGIGLQFYFIFWQ 199
           + L  +  S    P GLY+YG+VG+GKTM+MDMFY   E  +K ++R+         F  
Sbjct: 116 EGLFSKLFSRSKPPRGLYVYGDVGTGKTMVMDMFYAYVE--MKRKKRVH--------FHG 175

Query: 200 AMLKINEDMHKIWKNQVREKPSQSSISSWIMNLPFDTKVKEWLAAEEKYKQEQADPVGAS 259
            ML +++ +H++ ++  + KP             F  K  + +A   +   E+     A 
Sbjct: 176 FMLDVHKRIHRLKQSLPKRKPG------------FMAKSYDPIAPIAEEISEE-----AC 235

Query: 260 ILCFDEIQTVDVFAIVALSGIVSRLLSTGTVLVATSNRAPKDLNQDGMQKDIFQKFVAKL 319
           +LCFDE Q  D+   + L  +   L   G V+VATSNR P+DL ++G+Q+  F  F+A L
Sbjct: 236 LLCFDEFQVTDIADAMILKQLFENLFKNGVVVVATSNRPPEDLYKNGLQRANFVPFIAVL 295

Query: 320 EEHCKFVLMEVH--------------YFWPLDCTSVGKFEKMWVEITRQLGGQITSETIP 379
           +E+C  V ++                Y+   +       +K++ E+  Q    +T   I 
Sbjct: 296 KEYCNTVQLDSGIDYRKRELPAAGKLYYLTSEADVEAVMDKLFDELA-QKQNDLTRPRIL 355

Query: 380 VMFGRKLEVSESCNGVARFAFDFLCGQPVGAADYIALAENYHTVFISNIPMMSMCIRDKA 439
            + GR+L ++++C  VA   F+ LC +P+GA+DY+ L++N+ T+F+ NIP  ++  R + 
Sbjct: 356 KVQGRELRLNKACGTVADCTFEELCERPLGASDYLELSKNFDTIFLRNIPQFTLANRTQG 415

Query: 440 RRFIALIDELYNHHCCLFCLAATSIEDLFQGTEEGALFDLERGRRGKASSKCPSGRQCRL 499
           RRFI LID  Y+    + C A+T I  LF      +  +LE+ R               L
Sbjct: 416 RRFITLIDNFYDLKVRIICSASTPISSLFLHQHHDS--ELEQSR--------ILMDDLGL 472

Query: 500 CRAPTAIVSMLSGQEEMFAFRRAVSRLIEMQTPLY 521
            +     +SM +G+EE+FAF+R +SRL EMQT  Y
Sbjct: 476 SQDSAEGLSMFTGEEEIFAFQRTISRLTEMQTEQY 472

BLAST of Sgr017818 vs. ExPASy Swiss-Prot
Match: P46441 (Putative ATPase N2B OS=Haematobia irritans OX=7368 PE=2 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 7.5e-42
Identity = 122/386 (31.61%), Postives = 187/386 (48.45%), Query Frame = 0

Query: 152 APTGLYIYGNVGSGKTMLMDMFYNATEGVVKHRRRIGIGLQFYFIFWQAMLKINEDMHKI 211
           AP G+YIYG+VG GKT LMDMFY+  + + K +R           F   M K++  +HK+
Sbjct: 109 APKGMYIYGSVGGGKTTLMDMFYSCCDDIPKKQR---------VHFNSFMSKVHGLIHKV 168

Query: 212 WKNQVREKPSQSSISSWIMNLPFD--TKVKEWLAAEEKYKQEQADPVGASILCFDEIQTV 271
                +E+  Q    +    LPFD    V E +A E            + ++CFDE Q  
Sbjct: 169 ----KQERGPQDRAFNSEKPLPFDPTLPVAEMIANE------------SWLICFDEFQVT 228

Query: 272 DVFAIVALSGIVSRLLSTGTVLVATSNRAPKDLNQDGMQKDIFQKFVAKLEEHCKFVLM- 331
           D+   + L  + + L + G V +ATSNR P DL ++G+Q+  F  F+  L   C    M 
Sbjct: 229 DIADAMILKSLFTHLFNEGIVCIATSNRHPNDLYKNGLQRSNFIPFIGVLLNRCNVAAMD 288

Query: 332 ------------EVHYFWPLDCTSVGKFEKMWVEITRQLGGQITSETIPVMFGRKLEVSE 391
                       + +YF      +  + E+M+  +  Q    I   TI   FGR L    
Sbjct: 289 SGVDYRRIAQSGDTNYFVTTQTDAKSQMERMFKILCSQENDIIRPRTI-THFGRDLTFQR 348

Query: 392 SCNGVARFAFDFLCGQPVGAADYIALAENYHTVFISNIPMMSMCIRDKARRFIALIDELY 451
           +C  V    F+ LC +P+G +DYI + + +HTV I ++P +++ ++ + RRFI LID LY
Sbjct: 349 TCGQVLDSNFEELCNRPLGGSDYIQIGQFFHTVLIHDVPQLTLLLKSQMRRFITLIDTLY 408

Query: 452 NHHCCLFCLAATSIEDLFQGTEEGALFDLERGRRGKASSKCPSGRQCRLCRAPTAIVSML 511
           ++   +   A   ++ LF  T++    DL   +R             +L    T+  S+ 
Sbjct: 409 DNRVRVVISAEVPLDQLFSFTDKPK--DLADEQRMLMD-------DLKLGDTDTS-ASVF 458

Query: 512 SGQEEMFAFRRAVSRLIEMQTPLYLE 523
           +G+EEMFAF R +SRL EMQ   Y E
Sbjct: 469 TGEEEMFAFDRTISRLYEMQKKEYWE 458

BLAST of Sgr017818 vs. ExPASy Swiss-Prot
Match: Q5TYS0 (Lactation elevated protein 1 homolog B OS=Danio rerio OX=7955 GN=lace1b PE=2 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 9.8e-42
Identity = 123/401 (30.67%), Postives = 199/401 (49.63%), Query Frame = 0

Query: 148 SAPHAPTGLYIYGNVGSGKTMLMDMFYNATEGVVKHRRRIGIGLQFYFIFWQAMLKINED 207
           S PH P G YIYGNVG+GKTMLMD+FY+  E   + ++R+         F   ML ++  
Sbjct: 147 SKPHPPQGYYIYGNVGTGKTMLMDLFYSFVEN--RRKKRVH--------FNGFMLDVHRR 206

Query: 208 MHKIWKNQVREKPSQSSISSWIMNLPFDTKVKEWLAAEEKYKQEQADPVGASILCFDEIQ 267
           +HK+ ++  + +  + ++   I  +  +        AEE             ++CFDE Q
Sbjct: 207 IHKLKQSLPKRRIGKMTMYDPIFPVAME-------IAEE-----------TCLICFDEFQ 266

Query: 268 TVDVFAIVALSGIVSRLLSTGTVLVATSNRAPKDLNQDGMQKDIFQKFVAKLEEHCKFVL 327
            VD+   + L  +   L   G V+VATSNR P++L ++G+Q+  F  F+  L+E+C+ V 
Sbjct: 267 VVDIADAMILKQLFEGLFKCGVVVVATSNRPPEELYKNGLQRAAFVPFIGVLKEYCRIVS 326

Query: 328 MEVH--------------YFWPLDCTSVGKFEKMWVEIT-RQLGGQITSETIPVMFGRKL 387
           ++                Y+   +  +      ++ E+  RQ    +T   +  + GR++
Sbjct: 327 LDTGIDFRTREMKPAGRLYYISSEPDAENAVNALFEELAFRQ--NDVTRPRVLNVQGREV 386

Query: 388 EVSESCNGVARFAFDFLCGQPVGAADYIALAENYHTVFISNIPMMSMCIRDKARRFIALI 447
            +S +C  +A  +F  LC QP+GA DY+ +A  + TV I N+P + + ++D+ARRF  LI
Sbjct: 387 TLSRTCGTIADCSFQELCEQPLGAGDYLEIARCFDTVIIRNVPYLQLGMKDQARRFTTLI 446

Query: 448 DELYNHHCCLFCLAATSIEDLF---QGTEEGALFDL---ERGRRGKASSKCPSGRQCRLC 507
           D  Y+    +  LA   ++ L    Q T E A   L   E G   +AS +          
Sbjct: 447 DNFYDQKVRVVMLADAPLDRLLDQGQMTGEEARDRLMLDELGLTDEASKR---------- 501

Query: 508 RAPTAIVSMLSGQEEMFAFRRAVSRLIEMQTPLY-LEGVRS 527
                 +++ +  EE+FAF+R VSRL EMQT  Y + G RS
Sbjct: 507 ------ITLFTADEEIFAFQRTVSRLAEMQTEQYWISGDRS 501

BLAST of Sgr017818 vs. ExPASy TrEMBL
Match: A0A6J1C362 (AFG1-like ATPase isoform X1 OS=Momordica charantia OX=3673 GN=LOC111007517 PE=3 SV=1)

HSP 1 Score: 819.3 bits (2115), Expect = 9.6e-234
Identity = 428/524 (81.68%), Postives = 443/524 (84.54%), Query Frame = 0

Query: 69  VKLANWKQNRENERRRLLTEEAESKQQGDVWTSVNKRRSKLVETLMFRKKSENIEPGVGK 128
           VKLANWKQNRENERR+LL EEAESKQQGDVWTSVNKR+SKLVETLMFR KSENIEPGVGK
Sbjct: 125 VKLANWKQNRENERRKLLMEEAESKQQGDVWTSVNKRQSKLVETLMFRNKSENIEPGVGK 184

Query: 129 WVSYLNRERKLDSLVGRCPSAPHAPTGLYIYGNVGSGKTMLMDMFYNATEGVVKHRRRIG 188
           WVSYLNRERKLDSLVGRCPSAPHAP GLYIYGNVGSGKTMLMDMFYNAT+G+VKHRRR  
Sbjct: 185 WVSYLNRERKLDSLVGRCPSAPHAPRGLYIYGNVGSGKTMLMDMFYNATKGIVKHRRR-- 244

Query: 189 IGLQFYFIFWQAMLKINEDMHKIWKNQVREKPSQSSISSWIMNLPFDTKVKEWLAAEEKY 248
                 + F +AMLKINEDMH+IWKNQVREK SQSSISSWIMNLPFDTKVKEWLAAEEKY
Sbjct: 245 ------YHFHEAMLKINEDMHQIWKNQVREKSSQSSISSWIMNLPFDTKVKEWLAAEEKY 304

Query: 249 KQE------------------QADPVGASILCFDEIQTVDVFAIVALSGIVSRLLSTGTV 308
           KQE                  QAD VGASILCFDEIQTVDVFAIVALSGIVSRLLSTGTV
Sbjct: 305 KQEVQMKNILPAVADKFLVDQQADQVGASILCFDEIQTVDVFAIVALSGIVSRLLSTGTV 364

Query: 309 LVATSNRAPKDLNQDGMQKDIFQKFVAKLEEHCKFVLM----------------EVHYFW 368
           LVATSNRAPKDLNQDGMQKDIFQKFVAKLEEHC+FVL+                +VHYFW
Sbjct: 365 LVATSNRAPKDLNQDGMQKDIFQKFVAKLEEHCEFVLIGSEIDYRRFIAQRSFDQVHYFW 424

Query: 369 PLDCTSVGKFEKMWVEITRQLGGQITSETIPVMFGRKLEVSESCNGVARFAFDFLCGQPV 428
           PL+ TSVGKFEKMW E+TRQLGGQITSETI VMFGRKLEVSESCNGVARFAFDFLCGQPV
Sbjct: 425 PLELTSVGKFEKMWAEMTRQLGGQITSETISVMFGRKLEVSESCNGVARFAFDFLCGQPV 484

Query: 429 GAADYIALAENYHTVFISNIPMMSMCIRDKARRFIALIDELYNHHCCLFCLAATSIEDLF 488
           GAADYIALA+NYHTVFISNIPMMSM IRDKARRFI LIDELYNHHCCLFCLAATSIEDLF
Sbjct: 485 GAADYIALAQNYHTVFISNIPMMSMRIRDKARRFITLIDELYNHHCCLFCLAATSIEDLF 544

Query: 489 QGTEEGALFDLE------RGRRGKASSKCPSGRQCRLCRAPTAIVSMLSGQEEMFAFRRA 548
           QGTEEG LFDLE          GK               APTAIVSMLSGQEEMFAFRRA
Sbjct: 545 QGTEEGTLFDLESFQFETEAEGGKLRRNVLEEGNVGSVGAPTAIVSMLSGQEEMFAFRRA 604

Query: 549 VSRLIEMQTPLYLEGVRSLHPYFQRREQESLIDRATRFQSQLSS 553
           VSRLIEMQTPLYLEGVRSLHPYFQRREQESL+DRAT+F SQLSS
Sbjct: 605 VSRLIEMQTPLYLEGVRSLHPYFQRREQESLVDRATKFHSQLSS 640

BLAST of Sgr017818 vs. ExPASy TrEMBL
Match: A0A6J1FDZ4 (AFG1-like ATPase OS=Cucurbita moschata OX=3662 GN=LOC111443118 PE=3 SV=1)

HSP 1 Score: 808.1 bits (2086), Expect = 2.2e-230
Identity = 424/524 (80.92%), Postives = 436/524 (83.21%), Query Frame = 0

Query: 69  VKLANWKQNRENERRRLLTEEAESKQQGDVWTSVNKRRSKLVETLMFRKKSENIEPGVGK 128
           VKLANWKQ+RENERRRLL EEAESKQQGDVWTSVNKRRSKL ETLMFRK SENIEPGVGK
Sbjct: 90  VKLANWKQSRENERRRLLMEEAESKQQGDVWTSVNKRRSKLAETLMFRKSSENIEPGVGK 149

Query: 129 WVSYLNRERKLDSLVGRCPSAPHAPTGLYIYGNVGSGKTMLMDMFYNATEGVVKHRRRIG 188
           WVSYLNRERKLDSLVGRCPSAPHAP GLYIYGNVGSGKTMLMDMFYNATEG+VKHR+R  
Sbjct: 150 WVSYLNRERKLDSLVGRCPSAPHAPRGLYIYGNVGSGKTMLMDMFYNATEGIVKHRQR-- 209

Query: 189 IGLQFYFIFWQAMLKINEDMHKIWKNQVREKPSQSSISSWIMNLPFDTKVKEWLAAEEKY 248
                 + F +AMLKINEDMHKIWKNQVREK SQSSISSWIMNLPFDTKVKEW+AAEEKY
Sbjct: 210 ------YHFHEAMLKINEDMHKIWKNQVREKSSQSSISSWIMNLPFDTKVKEWVAAEEKY 269

Query: 249 KQE------------------QADPVGASILCFDEIQTVDVFAIVALSGIVSRLLSTGTV 308
           KQE                  QA  VGASILCFDEIQTVDVFAIVALSGIVSRLLSTGTV
Sbjct: 270 KQEVQMKNILPAVADKFLVDQQAGQVGASILCFDEIQTVDVFAIVALSGIVSRLLSTGTV 329

Query: 309 LVATSNRAPKDLNQDGMQKDIFQKFVAKLEEHCKFVLM----------------EVHYFW 368
           LVATSNRAPKDLNQDGMQKDIFQKFV KLEEHC FV +                +VHYFW
Sbjct: 330 LVATSNRAPKDLNQDGMQKDIFQKFVTKLEEHCAFVFIGSEIDYRRFIAQRSFDQVHYFW 389

Query: 369 PLDCTSVGKFEKMWVEITRQLGGQITSETIPVMFGRKLEVSESCNGVARFAFDFLCGQPV 428
           P DCTSVGKFEKMWVEIT QLGGQITSETIPVMFGRKLEVSESCNGVARFAFDFLCGQPV
Sbjct: 390 PSDCTSVGKFEKMWVEITSQLGGQITSETIPVMFGRKLEVSESCNGVARFAFDFLCGQPV 449

Query: 429 GAADYIALAENYHTVFISNIPMMSMCIRDKARRFIALIDELYNHHCCLFCLAATSIEDLF 488
           GAADYIALA+NYHTVFIS+IPMMSM IRDKARRFI LIDELYNHHCCLFCLAATSIEDLF
Sbjct: 450 GAADYIALAKNYHTVFISDIPMMSMRIRDKARRFITLIDELYNHHCCLFCLAATSIEDLF 509

Query: 489 QGTEEGALFDLE------RGRRGKASSKCPSGRQCRLCRAPTAIVSMLSGQEEMFAFRRA 548
           QGTEEGALFDLE          GK               APTAIVSMLSGQEEMFAFRRA
Sbjct: 510 QGTEEGALFDLESFQFETEAEGGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRA 569

Query: 549 VSRLIEMQTPLYLEGVRSLHPYFQRREQESLIDRATRFQSQLSS 553
           VSRLIEMQTPLYLEGVR+LHPYFQRRE ESL+D A RFQ Q SS
Sbjct: 570 VSRLIEMQTPLYLEGVRNLHPYFQRREPESLVDSAARFQFQQSS 605

BLAST of Sgr017818 vs. ExPASy TrEMBL
Match: A0A6J1IG43 (AFG1-like ATPase OS=Cucurbita maxima OX=3661 GN=LOC111476619 PE=3 SV=1)

HSP 1 Score: 802.0 bits (2070), Expect = 1.6e-228
Identity = 421/524 (80.34%), Postives = 434/524 (82.82%), Query Frame = 0

Query: 69  VKLANWKQNRENERRRLLTEEAESKQQGDVWTSVNKRRSKLVETLMFRKKSENIEPGVGK 128
           VKLANWKQNRENERRRLL EEAESKQ GDVWTSVNKRRSKL ETLMFRK SENIEPGVGK
Sbjct: 90  VKLANWKQNRENERRRLLMEEAESKQPGDVWTSVNKRRSKLAETLMFRKSSENIEPGVGK 149

Query: 129 WVSYLNRERKLDSLVGRCPSAPHAPTGLYIYGNVGSGKTMLMDMFYNATEGVVKHRRRIG 188
           WVSYLNRERKLDSLVGRCPSAPHAP GLYIYGNVGSGKTMLMDMFYNATEG+VKHR+R  
Sbjct: 150 WVSYLNRERKLDSLVGRCPSAPHAPRGLYIYGNVGSGKTMLMDMFYNATEGIVKHRQR-- 209

Query: 189 IGLQFYFIFWQAMLKINEDMHKIWKNQVREKPSQSSISSWIMNLPFDTKVKEWLAAEEKY 248
                 + F +AMLKINEDMHKIWKNQVREK SQSSISSWIMNLPFDTKVKEW+AAEEKY
Sbjct: 210 ------YHFHEAMLKINEDMHKIWKNQVREKSSQSSISSWIMNLPFDTKVKEWVAAEEKY 269

Query: 249 KQE------------------QADPVGASILCFDEIQTVDVFAIVALSGIVSRLLSTGTV 308
           KQE                  QA  VGASILCFDEIQTVDVFAIVALSGIVSRLLSTGTV
Sbjct: 270 KQEVQMKNILPAVADKFLVDQQAGQVGASILCFDEIQTVDVFAIVALSGIVSRLLSTGTV 329

Query: 309 LVATSNRAPKDLNQDGMQKDIFQKFVAKLEEHCKFVLM----------------EVHYFW 368
           LVATSNRAPKDLNQDGMQKDIFQKFV KLEEHC FVL+                ++HYFW
Sbjct: 330 LVATSNRAPKDLNQDGMQKDIFQKFVTKLEEHCAFVLIGSEIDYRRFIAQRSFDQMHYFW 389

Query: 369 PLDCTSVGKFEKMWVEITRQLGGQITSETIPVMFGRKLEVSESCNGVARFAFDFLCGQPV 428
           P DCTSVGKFEKMWVEIT QLGGQITSETIPVMFGRKLEVSESCNGVARFAFDFLCGQPV
Sbjct: 390 PSDCTSVGKFEKMWVEITSQLGGQITSETIPVMFGRKLEVSESCNGVARFAFDFLCGQPV 449

Query: 429 GAADYIALAENYHTVFISNIPMMSMCIRDKARRFIALIDELYNHHCCLFCLAATSIEDLF 488
           GAADYIALA+NYHTVFIS+IP MSM IRDKARRFI LIDELYNHHCCLFCLAATSIEDLF
Sbjct: 450 GAADYIALAKNYHTVFISDIPTMSMRIRDKARRFITLIDELYNHHCCLFCLAATSIEDLF 509

Query: 489 QGTEEGALFDLE------RGRRGKASSKCPSGRQCRLCRAPTAIVSMLSGQEEMFAFRRA 548
           QGTEEGALFDLE          GK               APTAIVSMLSGQEEMFAFRRA
Sbjct: 510 QGTEEGALFDLESFQFETEAEGGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRA 569

Query: 549 VSRLIEMQTPLYLEGVRSLHPYFQRREQESLIDRATRFQSQLSS 553
           VSRLIEMQTPLYL+GVR+LHPYFQRRE  SL+D A RFQ Q SS
Sbjct: 570 VSRLIEMQTPLYLKGVRNLHPYFQRREPASLVDSAARFQFQQSS 605

BLAST of Sgr017818 vs. ExPASy TrEMBL
Match: A0A5A7TBP6 (Lactation elevated protein 1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold92G003620 PE=3 SV=1)

HSP 1 Score: 774.6 bits (1999), Expect = 2.7e-220
Identity = 426/638 (66.77%), Postives = 458/638 (71.79%), Query Frame = 0

Query: 24  SGSCDVLFFQNLRAHCSETEKKKEVRFENEKRTFIFNNFFATVAK--------------- 83
           SG+  +LFFQ        ++K+K++RFE+EKRTF F NFF TVA+               
Sbjct: 26  SGNSYILFFQKDYTPYFASKKEKKLRFEDEKRTFDFINFFPTVAEFDFFSLCFPMEGSQV 85

Query: 84  ----------------------------------------------------------VK 143
                                                                     VK
Sbjct: 86  VFRTCNLKSILSGLLDKYRVLVERGELQHDPFQERVASELESLLGRLEQYEKDMEDYHVK 145

Query: 144 LANWKQNRENERRRLLTEEAESKQQGDVWTSVNKRRSKLVETLMFRKKSENIEPGVGKWV 203
           LA W+QNRE ERRRLL EEAESKQQGD   +VNKRRSKL ETLMFRKK+ENIEPGVGKWV
Sbjct: 146 LAEWEQNREKERRRLLMEEAESKQQGD---AVNKRRSKLFETLMFRKKAENIEPGVGKWV 205

Query: 204 SYLNRERKLDSLVGRCPSAPHAPTGLYIYGNVGSGKTMLMDMFYNATEGVVKHRRRIGIG 263
           SYLNRE+KLDSLVGRCP+APHAP GLYIYGNVGSGKTMLMDMF+NAT+G+VKHR+R    
Sbjct: 206 SYLNREKKLDSLVGRCPAAPHAPRGLYIYGNVGSGKTMLMDMFFNATQGIVKHRQR---- 265

Query: 264 LQFYFIFWQAMLKINEDMHKIWKNQVREKPSQSSISSWIMNLPFDTKVKEWLAAEEKYKQ 323
               + F +AMLKINEDMHKIWKNQVREK SQSS SSWIMNLPFDTKVKEWLA EEKYKQ
Sbjct: 266 ----YHFHEAMLKINEDMHKIWKNQVREKSSQSSFSSWIMNLPFDTKVKEWLAGEEKYKQ 325

Query: 324 E------------------QADPVGASILCFDEIQTVDVFAIVALSGIVSRLLSTGTVLV 383
           E                  QA  VGASILCFDEIQTVDVFAIVALSGI+SRLLSTGTVLV
Sbjct: 326 EVQMKHILPAVADKFLIDQQAGHVGASILCFDEIQTVDVFAIVALSGIISRLLSTGTVLV 385

Query: 384 ATSNRAPKDLNQDGMQKDIFQKFVAKLEEHCKFVLM----------------EVHYFWPL 443
           ATSNRAP DLNQDGMQKDIFQKFV KLEEHC+FVL+                +VHYFWPL
Sbjct: 386 ATSNRAPNDLNQDGMQKDIFQKFVIKLEEHCEFVLIGSEIDYRRFIAQRSFDQVHYFWPL 445

Query: 444 DCTSVGKFEKMWVEITRQLGGQITSETIPVMFGRKLEVSESCNGVARFAFDFLCGQPVGA 503
           DCTSV KFE MWVE+T QLGGQITSET+PVMFGRKLEV ESCNGVARFAFDFLCGQPVGA
Sbjct: 446 DCTSVRKFENMWVEVTSQLGGQITSETVPVMFGRKLEVPESCNGVARFAFDFLCGQPVGA 505

Query: 504 ADYIALAENYHTVFISNIPMMSMCIRDKARRFIALIDELYNHHCCLFCLAATSIEDLFQG 548
           ADYIALA+NYHTVFISNIP+MSM IRDKARRFI LIDELYNHHCCLFCLAATSIE+LFQG
Sbjct: 506 ADYIALAKNYHTVFISNIPIMSMRIRDKARRFITLIDELYNHHCCLFCLAATSIEELFQG 565

BLAST of Sgr017818 vs. ExPASy TrEMBL
Match: A0A5D3E5L9 (Lactation elevated protein 1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G007380 PE=3 SV=1)

HSP 1 Score: 774.2 bits (1998), Expect = 3.5e-220
Identity = 425/638 (66.61%), Postives = 458/638 (71.79%), Query Frame = 0

Query: 24  SGSCDVLFFQNLRAHCSETEKKKEVRFENEKRTFIFNNFFATVAK--------------- 83
           SG+  +LFFQ        ++K+K++RFE+EKRTF F NFF TVA+               
Sbjct: 26  SGNSYILFFQKDYTPYFASKKEKKLRFEDEKRTFDFVNFFPTVAEFDFFSLCFPMEGSQV 85

Query: 84  ----------------------------------------------------------VK 143
                                                                     VK
Sbjct: 86  VFRTCNLKSILSGLLDKYRVLVERGELQHDPFQERVASELESLLGRLEQYEKDMEDYHVK 145

Query: 144 LANWKQNRENERRRLLTEEAESKQQGDVWTSVNKRRSKLVETLMFRKKSENIEPGVGKWV 203
           LA W+QNRE ERRRLL EEAESKQQGD   +VNKRRSKL ETLMFRKK+ENIEPGVGKWV
Sbjct: 146 LAEWEQNREKERRRLLMEEAESKQQGD---AVNKRRSKLFETLMFRKKAENIEPGVGKWV 205

Query: 204 SYLNRERKLDSLVGRCPSAPHAPTGLYIYGNVGSGKTMLMDMFYNATEGVVKHRRRIGIG 263
           SYLNRE+KLDSL+GRCP+APHAP GLYIYGNVGSGKTMLMDMF+NAT+G+VKHR+R    
Sbjct: 206 SYLNREKKLDSLIGRCPAAPHAPRGLYIYGNVGSGKTMLMDMFFNATQGIVKHRQR---- 265

Query: 264 LQFYFIFWQAMLKINEDMHKIWKNQVREKPSQSSISSWIMNLPFDTKVKEWLAAEEKYKQ 323
               + F +AMLKINEDMHKIWKNQVREK SQSS SSWIMNLPFDTKVKEWLA EEKYKQ
Sbjct: 266 ----YHFHEAMLKINEDMHKIWKNQVREKSSQSSFSSWIMNLPFDTKVKEWLAGEEKYKQ 325

Query: 324 E------------------QADPVGASILCFDEIQTVDVFAIVALSGIVSRLLSTGTVLV 383
           E                  QA  VGASILCFDEIQTVDVFAIVALSGI+SRLLSTGTVLV
Sbjct: 326 EVQMKHILPAVADKFLIDQQAGHVGASILCFDEIQTVDVFAIVALSGIISRLLSTGTVLV 385

Query: 384 ATSNRAPKDLNQDGMQKDIFQKFVAKLEEHCKFVLM----------------EVHYFWPL 443
           ATSNRAP DLNQDGMQKDIFQKFV KLEEHC+FVL+                +VHYFWPL
Sbjct: 386 ATSNRAPNDLNQDGMQKDIFQKFVIKLEEHCEFVLIGSEIDYRRFIAQRSFDQVHYFWPL 445

Query: 444 DCTSVGKFEKMWVEITRQLGGQITSETIPVMFGRKLEVSESCNGVARFAFDFLCGQPVGA 503
           DCTSV KFE MWVE+T QLGGQITSET+PVMFGRKLEV ESCNGVARFAFDFLCGQPVGA
Sbjct: 446 DCTSVRKFENMWVEVTSQLGGQITSETVPVMFGRKLEVPESCNGVARFAFDFLCGQPVGA 505

Query: 504 ADYIALAENYHTVFISNIPMMSMCIRDKARRFIALIDELYNHHCCLFCLAATSIEDLFQG 548
           ADYIALA+NYHTVFISNIP+MSM IRDKARRFI LIDELYNHHCCLFCLAATSIE+LFQG
Sbjct: 506 ADYIALAKNYHTVFISNIPIMSMRIRDKARRFITLIDELYNHHCCLFCLAATSIEELFQG 565

BLAST of Sgr017818 vs. TAIR 10
Match: AT2G25530.1 (AFG1-like ATPase family protein )

HSP 1 Score: 599.4 bits (1544), Expect = 3.0e-171
Identity = 307/507 (60.55%), Postives = 370/507 (72.98%), Query Frame = 0

Query: 69  VKLANWKQNRENERRRLLTEEAESKQQGDVWTSVNKRRSKLVETLMFRKKSENIEPGVGK 128
           V+LA W++ RE ERR+L+ EEAE K+   +W SVNK+  KL+   +  ++  N+EPGVGK
Sbjct: 157 VRLAEWEKKREEERRKLMVEEAEKKEDDGMWASVNKQGQKLLGRWVLGRRQMNVEPGVGK 216

Query: 129 WVSYLNRERKLDSLVGRCPSAPHAPTGLYIYGNVGSGKTMLMDMFYNATEGVVKHRRRIG 188
           WVSYLNRERKLDS+VG  P+ P AP GLYIYGNVG GKTMLMDMFY AT+G+++HR+R  
Sbjct: 217 WVSYLNRERKLDSIVGSRPAVPPAPKGLYIYGNVGCGKTMLMDMFYGATDGIIRHRQR-- 276

Query: 189 IGLQFYFIFWQAMLKINEDMHKIWKNQVREKPSQSSISSWIMNLPFDTKVKEWLAAEEKY 248
                 F F +AMLKINE MHK WK    EKP Q SISSWIMNLP D KVKEWLA EE Y
Sbjct: 277 ------FHFHEAMLKINEQMHKYWKENGAEKPMQYSISSWIMNLPVDEKVKEWLAGEEFY 336

Query: 249 KQ------------------EQADPVGASILCFDEIQTVDVFAIVALSGIVSRLLSTGTV 308
           KQ                  +Q+   GASILCFDEIQTVDVFAIVALSGI+SRLL+TGTV
Sbjct: 337 KQQLQMKHILPAVADKFLVDQQSSKKGASILCFDEIQTVDVFAIVALSGIMSRLLTTGTV 396

Query: 309 LVATSNRAPKDLNQDGMQKDIFQKFVAKLEEHCKFVLM----------------EVHYFW 368
           LVATSNRAP++LNQDGMQK+IF KF++KLE+HC+ + +                 VHY W
Sbjct: 397 LVATSNRAPRELNQDGMQKEIFDKFISKLEKHCEIISIGSEVDYRRVAAKNSAENVHYLW 456

Query: 369 PLDCTSVGKFEKMWVEITRQLGGQITSETIPVMFGRKLEVSESCNGVARFAFDFLCGQPV 428
           PL+   + +FEKMW ++T Q GG+ITS T+PVMFGR +EV ESC+GVARF F++LCG+PV
Sbjct: 457 PLNDAVLEEFEKMWRQVTDQYGGEITSATLPVMFGRTVEVPESCSGVARFTFEYLCGRPV 516

Query: 429 GAADYIALAENYHTVFISNIPMMSMCIRDKARRFIALIDELYNHHCCLFCLAATSIEDLF 488
           GAADYIA+A+NYHT+FIS+IP MSM IRDKARRFI L+DELYNHHCCL   A T+I++LF
Sbjct: 517 GAADYIAVAKNYHTIFISDIPAMSMEIRDKARRFITLVDELYNHHCCLVSSAETAIDELF 576

Query: 489 QGTEEGALFDLERGRRGKASSKCPSGR------QCRLCRAPTAIVSMLSGQEEMFAFRRA 536
           QGT EG LFDLE  +    +      R            +P++IVSMLSG+EEMFAF RA
Sbjct: 577 QGTAEGTLFDLESFQFETETEDSRLRRDVLAEGSISAAGSPSSIVSMLSGEEEMFAFARA 636

BLAST of Sgr017818 vs. TAIR 10
Match: AT4G30490.1 (AFG1-like ATPase family protein )

HSP 1 Score: 142.1 bits (357), Expect = 1.3e-33
Identity = 116/395 (29.37%), Postives = 175/395 (44.30%), Query Frame = 0

Query: 147 PSAPHAPT-GLYIYGNVGSGKTMLMDMFYNATEGVVKHRRRIGIGLQFYFIFWQAMLKIN 206
           P   ++P  GLY+YG VG+GKTMLMD+F++      K +R           F   ML ++
Sbjct: 154 PQTSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPCTWKKQR---------IHFHDFMLSVH 213

Query: 207 EDMHKIWKNQVREKPSQSSISSWIMNLPFDTKVKEWLAAEEKYKQEQADPVGASILCFDE 266
             + K              +S  +                E   QE A    A +LC DE
Sbjct: 214 SRLQK-----------HKGLSDPL----------------EVVAQEIAH--DAILLCLDE 273

Query: 267 IQTVDVFAIVALSGIVSRLLSTGTVLVATSNRAPKDLNQDGMQKDIFQKFVAKLEEHCKF 326
               DV   + L+ +   L S G +LVATSNR P  L + G+Q+D+F  F++ L+E  + 
Sbjct: 274 FMVTDVADALILNRLFGHLFSNGVILVATSNRNPDKLYEGGLQRDLFLPFISSLKE--RS 333

Query: 327 VLMEV---------------HYFWPLDCTSVGKFEKMWVEITRQLGGQ---ITSETIPVM 386
           V+ E+                YF   D +++ K      +  RQL G       + + V+
Sbjct: 334 VVHEIGSAVDYRKLTSAEQGFYFIGKDLSTLLK------QKFRQLIGDNVVARPQVVEVV 393

Query: 387 FGRKLEVSESCNGVARFAFDFLCGQPVGAADYIALAENYHTVFISNIPMMSMCIRDKARR 446
            GRKL++    NG A F F+ LC +P+GAADY  L + +HT+ +  IP+  +  R  A R
Sbjct: 394 MGRKLQIPLGANGCAYFPFEELCDRPLGAADYFGLFKKFHTLALDEIPVFGLHNRTAAYR 453

Query: 447 FIALIDELYNHHCCLFCLAATSIEDLFQGTEEGALFDLERGRRGKASSKCPSGRQCRLCR 506
           F+ L+D +Y +   L C A  + ++L    E+       +    + SS+        LC 
Sbjct: 454 FVTLVDVMYENRARLLCTAEANPQELL---EKIITISEAKSMGPRTSSRSRKNDVTELC- 489

Query: 507 APTAIVSMLSGQEEMFAFRRAVSRLIEMQTPLYLE 523
                       E  FA  R +SRL EM +  YLE
Sbjct: 514 ---------VDNELGFAKDRTISRLTEMNSKEYLE 489

BLAST of Sgr017818 vs. TAIR 10
Match: AT4G28070.2 (AFG1-like ATPase family protein )

HSP 1 Score: 139.4 bits (350), Expect = 8.5e-33
Identity = 114/383 (29.77%), Postives = 177/383 (46.21%), Query Frame = 0

Query: 155 GLYIYGNVGSGKTMLMDMFYNATEGVVKHRRRIGIGLQFYFIFWQAMLKINEDMHKIWKN 214
           GLY+YG VG+GKTMLMD+F++      + +R           F   ML ++    ++ K+
Sbjct: 135 GLYLYGGVGTGKTMLMDLFFHQLPASWRTQR---------IHFHNFMLSVHS---RLQKH 194

Query: 215 QVREKPSQSSISSWIMNLPFDTKVKEWLAAEEKYKQEQADPVGASILCFDEIQTVDVFAI 274
           +  E P +      ++ L                  E AD   A +LC DE    DV   
Sbjct: 195 KGLEDPLE------VVGL------------------EIADE--AILLCLDEFMVNDVADA 254

Query: 275 VALSGIVSRLLSTGTVLVATSNRAPKDLNQDGMQKDIFQKFVAKLEEHCKFVLMEV---- 334
           + L+ +   L + G +LVATSNRAP +L + G+Q+D+F  F++ L+E C  V+ E+    
Sbjct: 255 LILNRLFRHLFNNGIILVATSNRAPDNLYEGGLQRDLFLPFISTLKERC--VVREIGSSV 314

Query: 335 -----------HYFWPLDCTSVGKFEKMWVEITRQLGGQITSETIPVMFGRKLEVSESCN 394
                       YF   D + + K +K  + +  Q  G    + + V+ GRKL+V  + +
Sbjct: 315 DYRKLTSAEEGFYFIGKDISGLLK-QKFQLLVGDQPAG---PQVVEVVMGRKLQVPLAAD 374

Query: 395 GVARFAFDFLCGQPVGAADYIALAENYHTVFISNIPMMSMCIRDKARRFIALIDELYNHH 454
           G A F F+ LC +P+GAADY+ L + +HT+ +  +P   +  R  A RF+ L+D +Y + 
Sbjct: 375 GCAYFLFEELCDRPLGAADYLGLFKKFHTLALEGVPFFGLHNRTAAYRFVTLVDVMYENK 434

Query: 455 CCLFCLAATSIEDLFQGTEEGALFDLERGRRGKASSKCPSGRQCRLCRAPTAIVSMLSGQ 514
             L C A  S  +L    E        +    + SS+        LC             
Sbjct: 435 ARLLCTAEGSPLELL---ERIVTISDAQQIAPRTSSRSRKSDDPDLC----------VDN 460

Query: 515 EEMFAFRRAVSRLIEMQTPLYLE 523
           E  FA  R +SRL EM +  YLE
Sbjct: 495 ELGFAKDRTISRLTEMNSKEYLE 460

BLAST of Sgr017818 vs. TAIR 10
Match: AT4G28070.1 (AFG1-like ATPase family protein )

HSP 1 Score: 124.8 bits (312), Expect = 2.2e-28
Identity = 112/383 (29.24%), Postives = 172/383 (44.91%), Query Frame = 0

Query: 155 GLYIYGNVGSGKTMLMDMFYNATEGVVKHRRRIGIGLQFYFIFWQAMLKINEDMHKIWKN 214
           GLY+YG VG+GKTMLMD+F++      + +R           F   ML ++    ++ K+
Sbjct: 135 GLYLYGGVGTGKTMLMDLFFHQLPASWRTQR---------IHFHNFMLSVHS---RLQKH 194

Query: 215 QVREKPSQSSISSWIMNLPFDTKVKEWLAAEEKYKQEQADPVGASILCFDEIQTVDVFAI 274
           +  E P +      ++ L                  E AD   A +LC DE    DV   
Sbjct: 195 KGLEDPLE------VVGL------------------EIADE--AILLCLDEFMVNDVADA 254

Query: 275 VALSGIVSRLLSTGTVLVATSNRAPKDLNQDGMQKDIFQKFVAKLEEHCKFVLMEV---- 334
           + L+ +   L + G +LVATSNRAP +L + G+Q+D+F  F++ L+E C  V+ E+    
Sbjct: 255 LILNRLFRHLFNNGIILVATSNRAPDNLYEGGLQRDLFLPFISTLKERC--VVREIGSSV 314

Query: 335 -----------HYFWPLDCTSVGKFEKMWVEITRQLGGQITSETIPVMFGRKLEVSESCN 394
                       YF   D + + K +K  + +  Q  G    + + V+ GRKL+V  + +
Sbjct: 315 DYRKLTSAEEGFYFIGKDISGLLK-QKFQLLVGDQPAG---PQVVEVVMGRKLQVPLAAD 374

Query: 395 GVARFAFDFLCGQPVGAADYIALAENYHTVFISNIPMMSMCIRDKARRFIALIDELYNHH 454
           G A F F+ LC +P+GAADY+ L          ++P   +  R  A RF+ L+D +Y + 
Sbjct: 375 GCAYFLFEELCDRPLGAADYLGL---------FSVPFFGLHNRTAAYRFVTLVDVMYENK 434

Query: 455 CCLFCLAATSIEDLFQGTEEGALFDLERGRRGKASSKCPSGRQCRLCRAPTAIVSMLSGQ 514
             L C A  S  +L    E        +    + SS+        LC             
Sbjct: 435 ARLLCTAEGSPLELL---ERIVTISDAQQIAPRTSSRSRKSDDPDLC----------VDN 451

Query: 515 EEMFAFRRAVSRLIEMQTPLYLE 523
           E  FA  R +SRL EM +  YLE
Sbjct: 495 ELGFAKDRTISRLTEMNSKEYLE 451