Sgr017624 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr017624
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Descriptionshort-chain dehydrogenase TIC 32, chloroplastic-like
Locationtig00153054: 217952 .. 220806 (+)
RNA-Seq ExpressionSgr017624
SyntenySgr017624
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGGAGACGCTTAAGTATTTGGCAGGTATTGCAGGCCCAAGTGGGTATGGCTCTAAATCCACAGCCGATCAAGTTATTGAACATTCTCAGTTTTCTTCTTCTTTGACTGCCATTATCACTGGTTAGTATCTACAACCTTTAACAAATGCAACTGTTTTAAGATCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTGCATGAGAGATTGTTCAAAGTGACATTATGCTAATAACCAAATTCTCCCTCTTGTATTAGTTTTTCTTTTTCTCACTTTTTTTTTCCGCAAGTTTTGCATTTGTTATGGTTTATTTTGGATGCTGTCAAGTTCAAAAGTTGTGGTTAATTTGCAGAGTTAAAAGCATCATTTTGTATAAGGGAGGTGACAAGAAACCGCGAAAGGGTTTGAACCAAACCGAATTATATGATTTAGATAGTGGTTTTTATTTAAAAATAAGTGGGAAACAGAAAAATGTGTGTTAAAAACCAAACTTACCACGAAGACCGTCTAGTTTGAGTTCTTGTTCCATTTTCATAATTTAGTTCATATCAACCCCCGAAACTAGCCGAATTGAACCATGATGAAGAGGGTCTAATTCAGTTGAATCTTATGGAATTGACCAATTTTGACCCTCATTTTTTTAGTGAAATCACATGCTATACAGACTTGTATAGAAAGTTTGATTATTGGGAATGAGGTGAAATCTGTCCAAACATCAATCAAAGCAGATATTATTGCCAAAGATAATATGAAAAGAACAGAGAAGTGGGGTTGAAGATAGCTCTTAAAACAAGACAAAAATCCCAAGAATGAAAACTAAAACAACATAGAAAGGTTGATTATTGAGTAGCAATAAATAATTTTGTGTGGATGAATTAAAGGGCAACATCAGGAATTGGAGAAGAAACAGCAAGAGTGATGGCAAAGAGAGGGGTGAGGATAGTAATGGCAGCGAGAGACAGGAAGAAGGCAGAAGAAGTGAAAGAGAGGATCAAAAGAGAGAGTCCAGAGGCAGAAATATTAGTGACAGAGATAGACCTGAGCTCGTTGGCTTCTGTGAGGAGGTTCTGTGTGGAGTTTGCAGCTTTAGGGCTACCACTCAACATTCTCATGTGAGTTTGCCAATGCTTTTCCCAACCAAACGCCATTTTTGGCTCTGCAAGCTCACCCCTTTTTGGACACGATTGTTTTCTTTTGCAGAAACAATGGTGGGGTTTTCTCTCCAAATTTGGAATATTCCGACGACAAAATTGAATTGACATTTGCTACTAATTATTTGGGTAATATTTTCTTTTCCCCATTCTTAAACTTAGAAAGAATTTGTATCATTAATTACAACCTTTCATCAATTTAAATATATAGTTATTTTGGTCCTTCTATCTTCGATTTTTTACCTATTTTAGTCTATATTTTCAGCTTTTGAAAATTGACTATTTTAGCTTTTTTTTTTTTTTATAAAATTCTAACACAAATTCTATATACAACGAAAACTCTTTAATACAAAACTAAGATAATATATCAAAGATTTGAGATCAAAATAGTTACTTTTTAAAAGTTAGACACATGACCAAAATGGACAAAATAGAAAATGCAAGACCAAAATGAATTTTTTGACAGCATAGAATTCAAAATGAATAAAAGCTAAAATATAGGGACCAAAAATAAAATTTCAAACTTTAAAAAAATTAAAACACTATATTATTAAATAGCTTTTGTACTTCTTTTAAATGCCTAAAGAATTGTTAAACGAGCTGTTCACTTCTCAGCTAAAGAAATGGAAAGAAGATGAAATTCAAAAACTTTGTTTTCTGCACAATTAATTCAAAAAAGATTATTGCCGTGTTTCACTCTCTTTTTTTCTTCCCCTCCACAGGGCATTACCTTCTGACAGAAATACTTTTAGAGAAATTGATAGAAACATCAAACAAAACAGGCATCCAAGGAAGAATAATAAACGTCTCTTCCATGGCCCACTGCTGGGTGAAGAAAGATGGTCTCTGCTTCAGTCACCTGCTCAACCCAAACAAGTGACAAAACTTACTCTCCTTCTAGATTTCTTTTCTTTCTGGGTCTTTTCTTTCTCAAGCTGGTTTCTGGTCAATAATCAGCCATGGTAGAGAAGCAAACTAAACCCTGTTTGGGATTTGGGATATGCAGATACAACGGCACGCGTGCTTATGCTCAGTCAAAACTGGCTTGCATTTTACATGCCAAGGAGGTGGCTAGACAGTTGAAGGTTAATCTTATCTTTCTTCTCCACCTTCTATGTTTCCTGAAAACAAACAGAGCCCAAGTTCTGAAATTCAGTCATCTTTACAGGAAAGAGATGCCAATGTAACAATCAATGCAGTACATCCAGGAATTGTAAAGACCGCAATTATCAGGGCCCATCGAGGGTTTATCACAGGCATGAACTATAGGTTTTGACTTTTGATCCCCATCCCAGCAGCACTATGATCTTGAACTAGTTCTACATAAAGGAAAATGCAGAAGAGAGTATTGAATTATTATAATGATTCTAACATCTTTTGCAGATTCTCTCTTTTTCATGGCATCAAAGTTACTAAAGTCCATTCCTCAGGTAAATTATATGTAAAGAAGTGCAGAGGGTTGGGATTTGGGTAATGGTTTTTCATACAAACAGCTAATGCAATGATCATGCATGCAGGGTGCTGCAACAACATGCTATGTGGCTTTAAGCCCACAAACAGAAAGAAAAACAGGAAAATATTTTGCAGATTGTAATGAGACCAAGTGTTCAGCTCTAGCCAATGACAAGTTAGTAGCACGAAAGCTATGGATGCAAACTCGTGCCCTTGTTCTCAGACAACTTGGTGAACCAACGCCAGCTTAA

mRNA sequence

ATGAAGGAGACGCTTAAGTATTTGGCAGGTATTGCAGGCCCAAGTGGGTATGGCTCTAAATCCACAGCCGATCAAGTTATTGAACATTCTCAGTTTTCTTCTTCTTTGACTGCCATTATCACTGGAATTGGAGAAGAAACAGCAAGAGTGATGGCAAAGAGAGGGGTGAGGATAGTAATGGCAGCGAGAGACAGGAAGAAGGCAGAAGAAGTGAAAGAGAGGATCAAAAGAGAGAGTCCAGAGGCAGAAATATTAGTGACAGAGATAGACCTGAGCTCGTTGGCTTCTGTGAGGAGGTTCTGTGTGGAGTTTGCAGCTTTAGGGCTACCACTCAACATTCTCATAAACAATGGTGGGGTTTTCTCTCCAAATTTGGAATATTCCGACGACAAAATTGAATTGACATTTGCTACTAATTATTTGGGGCATTACCTTCTGACAGAAATACTTTTAGAGAAATTGATAGAAACATCAAACAAAACAGGCATCCAAGGAAGAATAATAAACGTCTCTTCCATGGCCCACTGCTGGGTGAAGAAAGATGGTCTCTGCTTCAGTCACCTGCTCAACCCAAACAAATACAACGGCACGCGTGCTTATGCTCAGTCAAAACTGGCTTGCATTTTACATGCCAAGGAGGTGGCTAGACAGTTGAAGGAAAGAGATGCCAATGTAACAATCAATGCAGTACATCCAGGAATTGTAAAGACCGCAATTATCAGGGCCCATCGAGGGTTTATCACAGATTCTCTCTTTTTCATGGCATCAAAGTTACTAAAGTCCATTCCTCAGGGTGCTGCAACAACATGCTATGTGGCTTTAAGCCCACAAACAGAAAGAAAAACAGGAAAATATTTTGCAGATTGTAATGAGACCAAGTGTTCAGCTCTAGCCAATGACAAGTTAGTAGCACGAAAGCTATGGATGCAAACTCGTGCCCTTGTTCTCAGACAACTTGGTGAACCAACGCCAGCTTAA

Coding sequence (CDS)

ATGAAGGAGACGCTTAAGTATTTGGCAGGTATTGCAGGCCCAAGTGGGTATGGCTCTAAATCCACAGCCGATCAAGTTATTGAACATTCTCAGTTTTCTTCTTCTTTGACTGCCATTATCACTGGAATTGGAGAAGAAACAGCAAGAGTGATGGCAAAGAGAGGGGTGAGGATAGTAATGGCAGCGAGAGACAGGAAGAAGGCAGAAGAAGTGAAAGAGAGGATCAAAAGAGAGAGTCCAGAGGCAGAAATATTAGTGACAGAGATAGACCTGAGCTCGTTGGCTTCTGTGAGGAGGTTCTGTGTGGAGTTTGCAGCTTTAGGGCTACCACTCAACATTCTCATAAACAATGGTGGGGTTTTCTCTCCAAATTTGGAATATTCCGACGACAAAATTGAATTGACATTTGCTACTAATTATTTGGGGCATTACCTTCTGACAGAAATACTTTTAGAGAAATTGATAGAAACATCAAACAAAACAGGCATCCAAGGAAGAATAATAAACGTCTCTTCCATGGCCCACTGCTGGGTGAAGAAAGATGGTCTCTGCTTCAGTCACCTGCTCAACCCAAACAAATACAACGGCACGCGTGCTTATGCTCAGTCAAAACTGGCTTGCATTTTACATGCCAAGGAGGTGGCTAGACAGTTGAAGGAAAGAGATGCCAATGTAACAATCAATGCAGTACATCCAGGAATTGTAAAGACCGCAATTATCAGGGCCCATCGAGGGTTTATCACAGATTCTCTCTTTTTCATGGCATCAAAGTTACTAAAGTCCATTCCTCAGGGTGCTGCAACAACATGCTATGTGGCTTTAAGCCCACAAACAGAAAGAAAAACAGGAAAATATTTTGCAGATTGTAATGAGACCAAGTGTTCAGCTCTAGCCAATGACAAGTTAGTAGCACGAAAGCTATGGATGCAAACTCGTGCCCTTGTTCTCAGACAACTTGGTGAACCAACGCCAGCTTAA

Protein sequence

MKETLKYLAGIAGPSGYGSKSTADQVIEHSQFSSSLTAIITGIGEETARVMAKRGVRIVMAARDRKKAEEVKERIKRESPEAEILVTEIDLSSLASVRRFCVEFAALGLPLNILINNGGVFSPNLEYSDDKIELTFATNYLGHYLLTEILLEKLIETSNKTGIQGRIINVSSMAHCWVKKDGLCFSHLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVKTAIIRAHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQTERKTGKYFADCNETKCSALANDKLVARKLWMQTRALVLRQLGEPTPA
Homology
BLAST of Sgr017624 vs. NCBI nr
Match: XP_023000696.1 (short-chain dehydrogenase TIC 32, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 568.9 bits (1465), Expect = 2.8e-158
Identity = 288/326 (88.34%), Postives = 309/326 (94.79%), Query Frame = 0

Query: 1   MKETLKYLAGIAGPSGYGSKSTADQVIEHSQFSSSLTAIIT----GIGEETARVMAKRGV 60
           MKETL+YL GIAGPSGYGSKS+A+QV EH Q SS LTAIIT    GIGEETARV+AKRGV
Sbjct: 14  MKETLRYLVGIAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGIGEETARVLAKRGV 73

Query: 61  RIVMAARDRKKAEEVKERIKRESPEAEILVTEIDLSSLASVRRFCVEFAALGLPLNILIN 120
           RIVMAARD KKA +V+ERIKRE PEAEILV+EIDLSSLASVRRFC+EFAALGLPLNILIN
Sbjct: 74  RIVMAARDMKKAAKVRERIKREGPEAEILVSEIDLSSLASVRRFCMEFAALGLPLNILIN 133

Query: 121 NGGVFSPNLEYSDDKIELTFATNYLGHYLLTEILLEKLIETSNKTGIQGRIINVSSMAHC 180
           NGGVFSPNLEYSDDK+ELTFATNYLGHYLLTE+LL+KLIETS +TGIQGRIINVSS+ HC
Sbjct: 134 NGGVFSPNLEYSDDKLELTFATNYLGHYLLTELLLDKLIETSRRTGIQGRIINVSSVVHC 193

Query: 181 WVKKDGLCFSHLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVK 240
           WVKKD LCF+HLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVK
Sbjct: 194 WVKKDDLCFTHLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVK 253

Query: 241 TAIIRAHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQTERKTGKYFADCNETKCSA 300
           TAII+AHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQT+RKTG+YFADCNETKCS+
Sbjct: 254 TAIIKAHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQTQRKTGRYFADCNETKCSS 313

Query: 301 LANDKLVARKLWMQTRALVLRQLGEP 323
           LAND+L AR LWMQTRAL+LR+LGEP
Sbjct: 314 LANDELAARTLWMQTRALILRELGEP 339

BLAST of Sgr017624 vs. NCBI nr
Match: XP_023519347.1 (short-chain dehydrogenase TIC 32, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 563.9 bits (1452), Expect = 8.9e-157
Identity = 284/326 (87.12%), Postives = 308/326 (94.48%), Query Frame = 0

Query: 1   MKETLKYLAGIAGPSGYGSKSTADQVIEHSQFSSSLTAIIT----GIGEETARVMAKRGV 60
           MKETL+YL GIAGPSGYGSKS+A+QV EH Q SS LTAIIT    GIGEETARV+AKRG+
Sbjct: 1   MKETLRYLVGIAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGIGEETARVLAKRGM 60

Query: 61  RIVMAARDRKKAEEVKERIKRESPEAEILVTEIDLSSLASVRRFCVEFAALGLPLNILIN 120
           RIVMAARD KKA +VKERIKRE PEAEILV+EIDLSSLASVRRFC+EFAALGLPLNILIN
Sbjct: 61  RIVMAARDMKKAAKVKERIKREGPEAEILVSEIDLSSLASVRRFCMEFAALGLPLNILIN 120

Query: 121 NGGVFSPNLEYSDDKIELTFATNYLGHYLLTEILLEKLIETSNKTGIQGRIINVSSMAHC 180
           NGGVFSPNLEYSDDK+ELTFATNYLGHY++TE+LL+KLIETS +TGIQGRIINVSS+ HC
Sbjct: 121 NGGVFSPNLEYSDDKLELTFATNYLGHYIMTELLLDKLIETSKRTGIQGRIINVSSVVHC 180

Query: 181 WVKKDGLCFSHLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVK 240
           WVKKD LCF+HLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVK
Sbjct: 181 WVKKDDLCFTHLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVK 240

Query: 241 TAIIRAHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQTERKTGKYFADCNETKCSA 300
           TAI++AHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQT RKTG+YFADCNETKCS+
Sbjct: 241 TAILKAHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQTRRKTGRYFADCNETKCSS 300

Query: 301 LANDKLVARKLWMQTRALVLRQLGEP 323
           LAND+L ARKLW+QTRAL+LR+L EP
Sbjct: 301 LANDELAARKLWIQTRALILRELDEP 326

BLAST of Sgr017624 vs. NCBI nr
Match: XP_022924073.1 (short-chain dehydrogenase TIC 32, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 561.6 bits (1446), Expect = 4.4e-156
Identity = 284/326 (87.12%), Postives = 307/326 (94.17%), Query Frame = 0

Query: 1   MKETLKYLAGIAGPSGYGSKSTADQVIEHSQFSSSLTAIIT----GIGEETARVMAKRGV 60
           MKETL+YL G+AGPSGYGSKS+A+QV EH Q SS LTAIIT    GIGEETARV+AKRGV
Sbjct: 14  MKETLRYLVGMAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGIGEETARVLAKRGV 73

Query: 61  RIVMAARDRKKAEEVKERIKRESPEAEILVTEIDLSSLASVRRFCVEFAALGLPLNILIN 120
           RIVMAARD KKA +V+ERIKR  PEAEIL++EIDLSSLASVRRFC+EFAALGLPLNILIN
Sbjct: 74  RIVMAARDMKKAAKVRERIKRGGPEAEILMSEIDLSSLASVRRFCMEFAALGLPLNILIN 133

Query: 121 NGGVFSPNLEYSDDKIELTFATNYLGHYLLTEILLEKLIETSNKTGIQGRIINVSSMAHC 180
           NGGVFSPNLEYSDDK+ELTFATNYLGHYLLTE+LL+KLIETS +TGIQGRIINVSS+ HC
Sbjct: 134 NGGVFSPNLEYSDDKLELTFATNYLGHYLLTELLLDKLIETSRRTGIQGRIINVSSVVHC 193

Query: 181 WVKKDGLCFSHLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVK 240
           WVKKD LCF+HLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVK
Sbjct: 194 WVKKDDLCFTHLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVK 253

Query: 241 TAIIRAHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQTERKTGKYFADCNETKCSA 300
           TAII+AHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQT RKTG+YFADCNETKCS+
Sbjct: 254 TAIIKAHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQTHRKTGRYFADCNETKCSS 313

Query: 301 LANDKLVARKLWMQTRALVLRQLGEP 323
           LAND+L AR LW+QTRAL+LR+LGEP
Sbjct: 314 LANDELAARTLWIQTRALILRELGEP 339

BLAST of Sgr017624 vs. NCBI nr
Match: XP_038895224.1 (short-chain dehydrogenase TIC 32 A, chloroplastic-like isoform X2 [Benincasa hispida])

HSP 1 Score: 555.4 bits (1430), Expect = 3.2e-154
Identity = 283/326 (86.81%), Postives = 306/326 (93.87%), Query Frame = 0

Query: 1   MKETLKYLAGIAGPSGYGSKSTADQVIEHSQFSSSLTAIIT----GIGEETARVMAKRGV 60
           MKETL+YL GIAGPSGYGSK++A++V E  QFSS  TAIIT    GIGEETARV+AKRGV
Sbjct: 1   MKETLRYLVGIAGPSGYGSKASAEKVTESFQFSSPFTAIITGATSGIGEETARVLAKRGV 60

Query: 61  RIVMAARDRKKAEEVKERIKRESPEAEILVTEIDLSSLASVRRFCVEFAALGLPLNILIN 120
           RIVMAARD KKA +VKERIKRESPEAEILV+EIDLSSLASVRRFCVEFAALGLPLNILIN
Sbjct: 61  RIVMAARDMKKAAKVKERIKRESPEAEILVSEIDLSSLASVRRFCVEFAALGLPLNILIN 120

Query: 121 NGGVFSPNLEYSDDKIELTFATNYLGHYLLTEILLEKLIETSNKTGIQGRIINVSSMAHC 180
           NGGVFSPNLEYS+DK ELTFATNYLGHYLLTE+LL+KLIETS +TGIQGRIINVSS+ H 
Sbjct: 121 NGGVFSPNLEYSEDKFELTFATNYLGHYLLTELLLDKLIETSKRTGIQGRIINVSSVVHY 180

Query: 181 WVKKDGLCFSHLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVK 240
           WVK++ LCF HLLNPNKYNGTRAYAQSKLACILHAKEVAR+LKE+DANVTINAVHPGIVK
Sbjct: 181 WVKREDLCFIHLLNPNKYNGTRAYAQSKLACILHAKEVARKLKEQDANVTINAVHPGIVK 240

Query: 241 TAIIRAHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQTERKTGKYFADCNETKCSA 300
           TAIIRAHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQT+RKTGKYFADCN+TKCS+
Sbjct: 241 TAIIRAHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQTQRKTGKYFADCNKTKCSS 300

Query: 301 LANDKLVARKLWMQTRALVLRQLGEP 323
           LAND+L AR LWMQTRAL+LR+LGEP
Sbjct: 301 LANDELTARTLWMQTRALILRELGEP 326

BLAST of Sgr017624 vs. NCBI nr
Match: XP_022137126.1 (short-chain dehydrogenase TIC 32, chloroplastic-like [Momordica charantia])

HSP 1 Score: 552.4 bits (1422), Expect = 2.7e-153
Identity = 284/329 (86.32%), Postives = 303/329 (92.10%), Query Frame = 0

Query: 1   MKETLKYLAGIAGPSGYGSKSTADQVIEHSQFSSSLTAIIT----GIGEETARVMAKRGV 60
           MKETL+YLAGIAG SGYGSKSTA+QV +HS FSS LTAIIT    GIGEETARVMAKRGV
Sbjct: 1   MKETLRYLAGIAGQSGYGSKSTAEQVTQHSHFSSPLTAIITGATSGIGEETARVMAKRGV 60

Query: 61  RIVMAARDRKKAEEVKERIKRESPEAEILVTEIDLSSLASVRRFCVEFAALGLPLNILIN 120
           RIV+AARD KKA +VKERIKRES EAEILV+EIDLSSLASVRRFCV+FAA GLPLNILIN
Sbjct: 61  RIVIAARDLKKAAKVKERIKRESAEAEILVSEIDLSSLASVRRFCVDFAASGLPLNILIN 120

Query: 121 NGGVFSPNLEYSDDKIELTFATNYLGHYLLTEILLEKLIETSNKTGIQGRIINVSSMAHC 180
           N GVFSPNLEYSDDK ELTFATNYLGHYLLTE+LL+ LIETS +TGIQGRIINVSS+ HC
Sbjct: 121 NAGVFSPNLEYSDDKFELTFATNYLGHYLLTEMLLDNLIETSKRTGIQGRIINVSSVVHC 180

Query: 181 WVKKDGLCFSHLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVK 240
           WVKKDGLCF+HLLNPNKYN T AYAQSKLACILHAKEVARQLKER ANVT+NA+HPGIVK
Sbjct: 181 WVKKDGLCFTHLLNPNKYNATLAYAQSKLACILHAKEVARQLKERAANVTVNALHPGIVK 240

Query: 241 TAIIRAHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQTERKTGKYFADCNETKCSA 300
           TAIIRAH GFITDSLFFMASKLLKSIPQGAATTCYVAL+PQTERKTGKYF+DCNETKCSA
Sbjct: 241 TAIIRAHGGFITDSLFFMASKLLKSIPQGAATTCYVALNPQTERKTGKYFSDCNETKCSA 300

Query: 301 LANDKLVARKLWMQTRALVLRQLGEPTPA 326
           LAN++L ARKLWMQTRAL+ RQLGE   A
Sbjct: 301 LANNELAARKLWMQTRALIHRQLGETASA 329

BLAST of Sgr017624 vs. ExPASy Swiss-Prot
Match: A0A078IS66 (Short-chain dehydrogenase TIC 32 A, chloroplastic OS=Brassica napus OX=3708 GN=TIC32A PE=2 SV=1)

HSP 1 Score: 270.4 bits (690), Expect = 2.7e-71
Identity = 149/305 (48.85%), Postives = 206/305 (67.54%), Query Frame = 0

Query: 8   LAGIAGPSGYGSKSTADQVIEHSQFSSSLTAIIT----GIGEETARVMAKRGVRIVMAAR 67
           + G AG SGYGS STA+ V  HS  +  LTAIIT    GIG E ARV+  RG  +++A+R
Sbjct: 7   VTGKAGKSGYGSASTAEDV-THSIDAKHLTAIITGGTSGIGLEAARVLGMRGAHVIIASR 66

Query: 68  DRKKAEEVKERIKRESPEAEILVTEIDLSSLASVRRFCVEFAALGLPLNILINNGGVFSP 127
           + K A + KE I +  P A I   ++DLSS+ SVR F  +F AL +PLNILINN GV   
Sbjct: 67  NTKAANDSKEMILQMYPNARIDCLQLDLSSIKSVRSFIHQFLALNVPLNILINNAGVMFC 126

Query: 128 NLEYSDDKIELTFATNYLGHYLLTEILLEKLIETSNKTGIQGRIINVSSMAHCWVKKDGL 187
             + S+D IE  FATN++GH+LLT +LL+K+  ++ ++GI+GRI+N+SS+AH +   +G+
Sbjct: 127 PFQLSEDGIESQFATNHIGHFLLTNLLLDKMKSSARESGIEGRIVNLSSIAHTYTYTEGI 186

Query: 188 CFSHLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVKTAIIRAH 247
            F ++ +P++Y+  +AY QSKLA +LH+  ++R+L+E   N+TIN+VHPG++ T + R H
Sbjct: 187 MFDYINDPDRYSEKKAYGQSKLANLLHSNALSRKLQEEGVNITINSVHPGLITTNLFR-H 246

Query: 248 RGFITDSLFFMASKLLKSIPQGAATTCYVALSPQTERKTGKYFADCNETKCSALANDKLV 307
            G     L  M+  L K+IPQGAATTCYVAL P  +  TGKYFADCN T  S  A D  +
Sbjct: 247 SGLGMAVLKAMSFFLWKNIPQGAATTCYVALHPDLKDVTGKYFADCNVTTPSNFAIDTTL 306

Query: 308 ARKLW 309
           A KLW
Sbjct: 307 ADKLW 309

BLAST of Sgr017624 vs. ExPASy Swiss-Prot
Match: A0A078ISJ6 (Short-chain dehydrogenase TIC 32 B, chloroplastic OS=Brassica napus OX=3708 GN=TIC32B PE=3 SV=1)

HSP 1 Score: 270.0 bits (689), Expect = 3.5e-71
Identity = 149/305 (48.85%), Postives = 205/305 (67.21%), Query Frame = 0

Query: 8   LAGIAGPSGYGSKSTADQVIEHSQFSSSLTAIIT----GIGEETARVMAKRGVRIVMAAR 67
           + G AG SGYGS STA+ V  HS  +  LTAIIT    GIG E ARV+  RG  +++AAR
Sbjct: 7   VTGKAGKSGYGSASTAEDV-THSIDAKHLTAIITGGTSGIGLEAARVLGMRGAHVIIAAR 66

Query: 68  DRKKAEEVKERIKRESPEAEILVTEIDLSSLASVRRFCVEFAALGLPLNILINNGGVFSP 127
           + K A + KE I +  P A I   ++DLSS+ SVR F  +F AL +PLNILINN GV   
Sbjct: 67  NTKAANDSKEMILQMYPNARIDCLQLDLSSIKSVRSFIHQFLALNVPLNILINNAGVMFC 126

Query: 128 NLEYSDDKIELTFATNYLGHYLLTEILLEKLIETSNKTGIQGRIINVSSMAHCWVKKDGL 187
             + S+D IE  FATN++GH+LLT +LL+K+  ++ ++GI+GRI+N+SS+AH +   +G+
Sbjct: 127 PFQLSEDGIESQFATNHIGHFLLTNLLLDKMKSSARESGIEGRIVNLSSIAHTYTYTEGI 186

Query: 188 CFSHLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVKTAIIRAH 247
            F ++ +P++Y+  +AY QSKLA +LH+  ++R+L+E   N+TIN+VHPG++ T + R H
Sbjct: 187 MFDYINDPDRYSEKKAYGQSKLANLLHSNALSRKLQEEGVNITINSVHPGLITTNLFR-H 246

Query: 248 RGFITDSLFFMASKLLKSIPQGAATTCYVALSPQTERKTGKYFADCNETKCSALANDKLV 307
            G     L  M+  L K+IPQGAATTCYVAL P  +  TGKYF DCN T  S  A D  +
Sbjct: 247 SGLGMAVLKAMSFFLWKNIPQGAATTCYVALHPDLKGVTGKYFTDCNVTTPSNFAIDTTL 306

Query: 308 ARKLW 309
           A KLW
Sbjct: 307 ADKLW 309

BLAST of Sgr017624 vs. ExPASy Swiss-Prot
Match: A2RVM0 (Short-chain dehydrogenase TIC 32, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC32 PE=2 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 6.1e-68
Identity = 143/316 (45.25%), Postives = 206/316 (65.19%), Query Frame = 0

Query: 10  GIAGPSGYGSKSTADQVIEHSQFSSSLTAIIT----GIGEETARVMAKRGVRIVMAARDR 69
           G  G SG+ S+STA++V  H    + LTAI+T    GIG ETARV++ RGV +VMA R+ 
Sbjct: 5   GSKGASGFSSRSTAEEV-THGVDGTGLTAIVTGASSGIGVETARVLSLRGVHVVMAVRNT 64

Query: 70  KKAEEVKERIKRESPEAEILVTEIDLSSLASVRRFCVEFAALGLPLNILINNGGVFSPNL 129
               +VKE I ++ P A++ V E+DLSS+ SVR+F  E+ + GLPLN+LINN G+ +   
Sbjct: 65  DSGAKVKEDIVKQVPGAKLDVMELDLSSMQSVRKFASEYKSTGLPLNLLINNAGIMACPF 124

Query: 130 EYSDDKIELTFATNYLGHYLLTEILLEKLIETSNKTGIQGRIINVSSMAHCWVKKDGLCF 189
             S D IEL FATN+LGH+LLT++LL+ +  TS ++  +GRI+N+SS AH +   +G+ F
Sbjct: 125 MLSKDNIELQFATNHLGHFLLTKLLLDTMKSTSRESKREGRIVNLSSEAHRFSYPEGVRF 184

Query: 190 SHLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVKTAIIRAHRG 249
             + + + Y+  RAY QSKL  +LHA E+ +QLKE   N+T N++HPG + T + R    
Sbjct: 185 DKINDKSSYSSMRAYGQSKLCNVLHANELTKQLKEDGVNITANSLHPGAIMTNLGRYFNP 244

Query: 250 FITDSLFFMASKLLKSIPQGAATTCYVALSPQTERKTGKYFADCNETKCSALANDKLVAR 309
           ++  ++  +A  +LKS+PQGAATTCYVAL+PQ    +G+YF D N  K   L  D  +A+
Sbjct: 245 YLAVAVGAVAKYILKSVPQGAATTCYVALNPQVAGVSGEYFQDSNIAKPLPLVKDTELAK 304

Query: 310 KLWMQTRALVLRQLGE 322
           K+W  +  L   Q GE
Sbjct: 305 KVWDFSTKLTDSQSGE 319

BLAST of Sgr017624 vs. ExPASy Swiss-Prot
Match: Q6RVV4 (Short-chain dehydrogenase TIC 32, chloroplastic OS=Pisum sativum OX=3888 GN=TIC32 PE=1 SV=1)

HSP 1 Score: 244.6 bits (623), Expect = 1.6e-63
Identity = 131/300 (43.67%), Postives = 195/300 (65.00%), Query Frame = 0

Query: 13  GPSGYGSKSTADQVIEHSQFSSSLTAIIT----GIGEETARVMAKRGVRIVMAARDRKKA 72
           G SG+   STA+QV  H   ++ LTAI+T    GIG ET RV+A RG  ++M  R+   A
Sbjct: 9   GVSGFSGSSTAEQV-THGIDATGLTAIVTGASSGIGAETTRVLALRGAHVIMGVRNMVAA 68

Query: 73  EEVKERIKRESPEAEILVTEIDLSSLASVRRFCVEFAALGLPLNILINNGGVFSPNLEYS 132
           ++VK+ I ++ P A++   E+DLSSL SV++F  EF + G PLNILINN G+ +   + S
Sbjct: 69  KDVKDTILKDIPSAKVDAIELDLSSLDSVKKFASEFNSSGRPLNILINNAGIMACPFKLS 128

Query: 133 DDKIELTFATNYLGHYLLTEILLEKLIETSNKTGIQGRIINVSSMAHCWVKKDGLCFSHL 192
            D IEL FATN++GH+LLT +LL+ + +T+ ++  +GRI+NV+S AH +   +G+ F  +
Sbjct: 129 KDNIELQFATNHIGHFLLTNLLLDTMKKTTRESKKEGRIVNVASEAHRFAYPEGIRFDKI 188

Query: 193 LNPNKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVKTAIIRAHRGFIT 252
            + + YN  RAY QSKLA +LHA ++ + LKE   N+T N++HPG + T + R H   + 
Sbjct: 189 NDQSSYNNWRAYGQSKLANVLHANQLTKHLKEDGVNITANSLHPGTIVTNLFR-HNSAVN 248

Query: 253 DSLFFMASKLLKSIPQGAATTCYVALSPQTERKTGKYFADCNETKCSALANDKLVARKLW 309
             +  +   +LK++ QGAATTCYVAL PQ +  +G+YF+D N  K +    D  +A+KLW
Sbjct: 249 GLINVIGKLVLKNVQQGAATTCYVALHPQVKGVSGEYFSDSNVYKTTPHGKDVDLAKKLW 306

BLAST of Sgr017624 vs. ExPASy Swiss-Prot
Match: Q9HBH5 (Retinol dehydrogenase 14 OS=Homo sapiens OX=9606 GN=RDH14 PE=1 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 2.9e-41
Identity = 111/295 (37.63%), Postives = 165/295 (55.93%), Query Frame = 0

Query: 36  LTAIITGIGEETARVMAKRGVRIVMAARDRKKAEEVKERIKRESPEA------------- 95
           +T   +G+G  TA  + + G R++M  RDR +AEE   +++RE  +A             
Sbjct: 48  ITGANSGLGRATAAELLRLGARVIMGCRDRARAEEAAGQLRRELRQAAECGPEPGVSGVG 107

Query: 96  EILVTEIDLSSLASVRRFCVEFAALGLPLNILINNGGVFSPNLEYSDDKIELTFATNYLG 155
           E++V E+DL+SL SVR FC E       L++LINN G+F      ++D  E+ F  N+LG
Sbjct: 108 ELIVRELDLASLRSVRAFCQEMLQEEPRLDVLINNAGIFQCPYMKTEDGFEMQFGVNHLG 167

Query: 156 HYLLTEILLEKLIETSNKTGIQGRIINVSSMAHCWVKKDGLCFSHLLNPNKYNGTRAYAQ 215
           H+LLT +LL  L     K+    RI+ VSS  +   K   + F  L +   YN +  Y++
Sbjct: 168 HFLLTNLLLGLL-----KSSAPSRIVVVSSKLY---KYGDINFDDLNSEQSYNKSFCYSR 227

Query: 216 SKLACILHAKEVARQLKERDANVTINAVHPGIVKTAIIR-AHRGFITDSLFFMAS-KLLK 275
           SKLA IL  +E+AR+L+    NVT+N +HPGIV+T + R  H   +   LF + S    K
Sbjct: 228 SKLANILFTRELARRLE--GTNVTVNVLHPGIVRTNLGRHIHIPLLVKPLFNLVSWAFFK 287

Query: 276 SIPQGAATTCYVALSPQTERKTGKYFADCNETKCSALANDKLVARKLWMQTRALV 316
           +  +GA T+ Y+A SP+ E  +G+YF DC E +    A D+ VARKLW  +  +V
Sbjct: 288 TPVEGAQTSIYLASSPEVEGVSGRYFGDCKEEELLPKAMDESVARKLWDISEVMV 332

BLAST of Sgr017624 vs. ExPASy TrEMBL
Match: A0A6J1KGJ5 (short-chain dehydrogenase TIC 32, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111495058 PE=3 SV=1)

HSP 1 Score: 568.9 bits (1465), Expect = 1.3e-158
Identity = 288/326 (88.34%), Postives = 309/326 (94.79%), Query Frame = 0

Query: 1   MKETLKYLAGIAGPSGYGSKSTADQVIEHSQFSSSLTAIIT----GIGEETARVMAKRGV 60
           MKETL+YL GIAGPSGYGSKS+A+QV EH Q SS LTAIIT    GIGEETARV+AKRGV
Sbjct: 14  MKETLRYLVGIAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGIGEETARVLAKRGV 73

Query: 61  RIVMAARDRKKAEEVKERIKRESPEAEILVTEIDLSSLASVRRFCVEFAALGLPLNILIN 120
           RIVMAARD KKA +V+ERIKRE PEAEILV+EIDLSSLASVRRFC+EFAALGLPLNILIN
Sbjct: 74  RIVMAARDMKKAAKVRERIKREGPEAEILVSEIDLSSLASVRRFCMEFAALGLPLNILIN 133

Query: 121 NGGVFSPNLEYSDDKIELTFATNYLGHYLLTEILLEKLIETSNKTGIQGRIINVSSMAHC 180
           NGGVFSPNLEYSDDK+ELTFATNYLGHYLLTE+LL+KLIETS +TGIQGRIINVSS+ HC
Sbjct: 134 NGGVFSPNLEYSDDKLELTFATNYLGHYLLTELLLDKLIETSRRTGIQGRIINVSSVVHC 193

Query: 181 WVKKDGLCFSHLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVK 240
           WVKKD LCF+HLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVK
Sbjct: 194 WVKKDDLCFTHLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVK 253

Query: 241 TAIIRAHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQTERKTGKYFADCNETKCSA 300
           TAII+AHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQT+RKTG+YFADCNETKCS+
Sbjct: 254 TAIIKAHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQTQRKTGRYFADCNETKCSS 313

Query: 301 LANDKLVARKLWMQTRALVLRQLGEP 323
           LAND+L AR LWMQTRAL+LR+LGEP
Sbjct: 314 LANDELAARTLWMQTRALILRELGEP 339

BLAST of Sgr017624 vs. ExPASy TrEMBL
Match: A0A6J1EBB9 (short-chain dehydrogenase TIC 32, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111431615 PE=3 SV=1)

HSP 1 Score: 561.6 bits (1446), Expect = 2.1e-156
Identity = 284/326 (87.12%), Postives = 307/326 (94.17%), Query Frame = 0

Query: 1   MKETLKYLAGIAGPSGYGSKSTADQVIEHSQFSSSLTAIIT----GIGEETARVMAKRGV 60
           MKETL+YL G+AGPSGYGSKS+A+QV EH Q SS LTAIIT    GIGEETARV+AKRGV
Sbjct: 14  MKETLRYLVGMAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGIGEETARVLAKRGV 73

Query: 61  RIVMAARDRKKAEEVKERIKRESPEAEILVTEIDLSSLASVRRFCVEFAALGLPLNILIN 120
           RIVMAARD KKA +V+ERIKR  PEAEIL++EIDLSSLASVRRFC+EFAALGLPLNILIN
Sbjct: 74  RIVMAARDMKKAAKVRERIKRGGPEAEILMSEIDLSSLASVRRFCMEFAALGLPLNILIN 133

Query: 121 NGGVFSPNLEYSDDKIELTFATNYLGHYLLTEILLEKLIETSNKTGIQGRIINVSSMAHC 180
           NGGVFSPNLEYSDDK+ELTFATNYLGHYLLTE+LL+KLIETS +TGIQGRIINVSS+ HC
Sbjct: 134 NGGVFSPNLEYSDDKLELTFATNYLGHYLLTELLLDKLIETSRRTGIQGRIINVSSVVHC 193

Query: 181 WVKKDGLCFSHLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVK 240
           WVKKD LCF+HLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVK
Sbjct: 194 WVKKDDLCFTHLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVK 253

Query: 241 TAIIRAHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQTERKTGKYFADCNETKCSA 300
           TAII+AHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQT RKTG+YFADCNETKCS+
Sbjct: 254 TAIIKAHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQTHRKTGRYFADCNETKCSS 313

Query: 301 LANDKLVARKLWMQTRALVLRQLGEP 323
           LAND+L AR LW+QTRAL+LR+LGEP
Sbjct: 314 LANDELAARTLWIQTRALILRELGEP 339

BLAST of Sgr017624 vs. ExPASy TrEMBL
Match: A0A6J1C5L3 (short-chain dehydrogenase TIC 32, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111008680 PE=3 SV=1)

HSP 1 Score: 552.4 bits (1422), Expect = 1.3e-153
Identity = 284/329 (86.32%), Postives = 303/329 (92.10%), Query Frame = 0

Query: 1   MKETLKYLAGIAGPSGYGSKSTADQVIEHSQFSSSLTAIIT----GIGEETARVMAKRGV 60
           MKETL+YLAGIAG SGYGSKSTA+QV +HS FSS LTAIIT    GIGEETARVMAKRGV
Sbjct: 1   MKETLRYLAGIAGQSGYGSKSTAEQVTQHSHFSSPLTAIITGATSGIGEETARVMAKRGV 60

Query: 61  RIVMAARDRKKAEEVKERIKRESPEAEILVTEIDLSSLASVRRFCVEFAALGLPLNILIN 120
           RIV+AARD KKA +VKERIKRES EAEILV+EIDLSSLASVRRFCV+FAA GLPLNILIN
Sbjct: 61  RIVIAARDLKKAAKVKERIKRESAEAEILVSEIDLSSLASVRRFCVDFAASGLPLNILIN 120

Query: 121 NGGVFSPNLEYSDDKIELTFATNYLGHYLLTEILLEKLIETSNKTGIQGRIINVSSMAHC 180
           N GVFSPNLEYSDDK ELTFATNYLGHYLLTE+LL+ LIETS +TGIQGRIINVSS+ HC
Sbjct: 121 NAGVFSPNLEYSDDKFELTFATNYLGHYLLTEMLLDNLIETSKRTGIQGRIINVSSVVHC 180

Query: 181 WVKKDGLCFSHLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVK 240
           WVKKDGLCF+HLLNPNKYN T AYAQSKLACILHAKEVARQLKER ANVT+NA+HPGIVK
Sbjct: 181 WVKKDGLCFTHLLNPNKYNATLAYAQSKLACILHAKEVARQLKERAANVTVNALHPGIVK 240

Query: 241 TAIIRAHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQTERKTGKYFADCNETKCSA 300
           TAIIRAH GFITDSLFFMASKLLKSIPQGAATTCYVAL+PQTERKTGKYF+DCNETKCSA
Sbjct: 241 TAIIRAHGGFITDSLFFMASKLLKSIPQGAATTCYVALNPQTERKTGKYFSDCNETKCSA 300

Query: 301 LANDKLVARKLWMQTRALVLRQLGEPTPA 326
           LAN++L ARKLWMQTRAL+ RQLGE   A
Sbjct: 301 LANNELAARKLWMQTRALIHRQLGETASA 329

BLAST of Sgr017624 vs. ExPASy TrEMBL
Match: A0A6J1E1M6 (short-chain dehydrogenase TIC 32, chloroplastic isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111429765 PE=3 SV=1)

HSP 1 Score: 480.3 bits (1235), Expect = 6.2e-132
Identity = 248/323 (76.78%), Postives = 283/323 (87.62%), Query Frame = 0

Query: 1   MKETLKYLAGIAGPSGYGSKSTADQVIEHSQFSSS--LTAIITGIGEETARVMAKRGVRI 60
           MK+TL+YLAGIAGPSGYGSKSTA+QV + S FSS+  LTAIITGIG ETARV+AKRGVRI
Sbjct: 17  MKDTLRYLAGIAGPSGYGSKSTAEQVTQCSSFSSTSQLTAIITGIGAETARVLAKRGVRI 76

Query: 61  VMAARDRKKAEEVKERIKRESPEAEILVTEIDLSSLASVRRFCVEFAALGLPLNILINNG 120
           VM ARD KKA +VKE I+RESPEAEI+V EIDLSSLASV+ FC  F AL LPLNILINN 
Sbjct: 77  VMPARDMKKAAQVKEAIQRESPEAEIIVCEIDLSSLASVQSFCNHFLALRLPLNILINNA 136

Query: 121 GVFSPNLEYSDDKIELTFATNYLGHYLLTEILLEKLIETSNKTGIQGRIINVSSMAHCWV 180
           GVFS NLE+S+DK+ELTFATNYLGHY LTE LLEK+IET+ KTGI+GRIIN+SS+ H WV
Sbjct: 137 GVFSKNLEFSEDKVELTFATNYLGHYFLTETLLEKMIETATKTGIEGRIINISSVVHGWV 196

Query: 181 KKDGLCFSHLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVKTA 240
           KKDGL F  +LNPN YNGTRAYAQSKLA ILHAKEV+RQL+ R+A VTINAVHPGIVKTA
Sbjct: 197 KKDGLNFGQMLNPNNYNGTRAYAQSKLANILHAKEVSRQLQGRNARVTINAVHPGIVKTA 256

Query: 241 IIRAHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQTERKTGKYFADCNETKCSALA 300
           IIRAH+GFITDSLFFMASKLLK+  QGA+TTCYVALS QTE K+GK++ADCNET CS+LA
Sbjct: 257 IIRAHKGFITDSLFFMASKLLKTTSQGASTTCYVALSSQTEGKSGKFYADCNETNCSSLA 316

Query: 301 NDKLVARKLWMQTRALVLRQLGE 322
           ND+L A+KLW +TR L+ +QL +
Sbjct: 317 NDELEAQKLWTRTRNLINKQLSK 339

BLAST of Sgr017624 vs. ExPASy TrEMBL
Match: A0A6J1JH35 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111485650 PE=3 SV=1)

HSP 1 Score: 478.4 bits (1230), Expect = 2.4e-131
Identity = 247/323 (76.47%), Postives = 283/323 (87.62%), Query Frame = 0

Query: 1   MKETLKYLAGIAGPSGYGSKSTADQVIEHSQFSSS--LTAIITGIGEETARVMAKRGVRI 60
           MK+TL+YLAGIAGPSGYGSKSTA+QV + S FSS+  LTAIITGIG ETARV+AKRGVRI
Sbjct: 17  MKDTLRYLAGIAGPSGYGSKSTAEQVTQCSSFSSTSQLTAIITGIGAETARVLAKRGVRI 76

Query: 61  VMAARDRKKAEEVKERIKRESPEAEILVTEIDLSSLASVRRFCVEFAALGLPLNILINNG 120
           VM ARD KKA +VKE I+RESPEAEI+V EIDLSSLASV+ FC  F AL LPLNILINN 
Sbjct: 77  VMPARDMKKAAQVKEAIQRESPEAEIIVCEIDLSSLASVQSFCNHFLALRLPLNILINNA 136

Query: 121 GVFSPNLEYSDDKIELTFATNYLGHYLLTEILLEKLIETSNKTGIQGRIINVSSMAHCWV 180
           GVFS NLE+S+DK+ELTFATNYLGHY LTE LLEK+IET+ +TGI+GRIINVSS+ H WV
Sbjct: 137 GVFSKNLEFSEDKVELTFATNYLGHYFLTETLLEKMIETAAQTGIEGRIINVSSVVHGWV 196

Query: 181 KKDGLCFSHLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVKTA 240
           KKDGL F  +LNPN YNGTRAYAQSKLA ILHAKEV+RQL+ R+A VTINAVHPGIVKTA
Sbjct: 197 KKDGLSFGQMLNPNNYNGTRAYAQSKLANILHAKEVSRQLQGRNARVTINAVHPGIVKTA 256

Query: 241 IIRAHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQTERKTGKYFADCNETKCSALA 300
           IIRAH+GFITDSLFFMASKLLK+  QGA+TTCYVALS QTE K+GK++ADCNET CS+LA
Sbjct: 257 IIRAHKGFITDSLFFMASKLLKTTSQGASTTCYVALSSQTEGKSGKFYADCNETNCSSLA 316

Query: 301 NDKLVARKLWMQTRALVLRQLGE 322
           ND++ A+KLW +TR L+ +QL +
Sbjct: 317 NDEIEAQKLWTRTRNLINKQLSK 339

BLAST of Sgr017624 vs. TAIR 10
Match: AT5G50130.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 436.0 bits (1120), Expect = 2.6e-122
Identity = 231/327 (70.64%), Postives = 275/327 (84.10%), Query Frame = 0

Query: 1   MKETLKYLAGIAGPSGYGSKSTADQVIEHSQF-SSSLTAIIT----GIGEETARVMAKRG 60
           MK TL+YLAGIAGP+G+GS+STA+QV +HS F  S LTAIIT    GIG ETARV+AKRG
Sbjct: 1   MKGTLRYLAGIAGPNGFGSRSTAEQVTQHSFFPCSHLTAIITGGTSGIGAETARVLAKRG 60

Query: 61  VRIVMAARDRKKAEEVKERIKRESPEAEILVTEIDLSSLASVRRFCVEFAALGLPLNILI 120
           VR+VMA RD KKAE VKERI RE+PEA+I++ EIDLSSL+SV RFC +F +  LPLNILI
Sbjct: 61  VRVVMAVRDMKKAEMVKERIIRENPEADIILFEIDLSSLSSVARFCSQFLSQDLPLNILI 120

Query: 121 NNGGVFSPNLEYSDDKIELTFATNYLGHYLLTEILLEKLIETSNKTGIQGRIINVSSMAH 180
           NN GVFSPNLE+S++KIELTFATN+LGHYLLTE+L+EK+I+T+ K+GI+GRIIN+SS+ H
Sbjct: 121 NNAGVFSPNLEFSEEKIELTFATNFLGHYLLTEMLIEKMIDTAEKSGIEGRIINLSSVIH 180

Query: 181 CWVKKDGLCFSHLLNP-NKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGI 240
            WVK D   F  LL+P ++YNGTRAYAQSKLA ILHAK +++QLK+R+ANVTINAVHPGI
Sbjct: 181 NWVKPDCFSFPKLLHPISRYNGTRAYAQSKLATILHAKALSKQLKDRNANVTINAVHPGI 240

Query: 241 VKTAIIRAHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQTERKTGKYFADCNETKC 300
           VKT IIRAH+G  TDSLF +ASKLLKSI QGAATTCYVALS +T+  +GKYFADCNET C
Sbjct: 241 VKTGIIRAHKGLFTDSLFLIASKLLKSISQGAATTCYVALSNETKGLSGKYFADCNETNC 300

Query: 301 SALANDKLVARKLWMQTRALVLRQLGE 322
           S LAND+ VA KL  Q+RAL+   L E
Sbjct: 301 SDLANDEYVALKLCTQSRALIHDHLHE 327

BLAST of Sgr017624 vs. TAIR 10
Match: AT5G50130.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 365.5 bits (937), Expect = 4.3e-101
Identity = 193/270 (71.48%), Postives = 231/270 (85.56%), Query Frame = 0

Query: 1   MKETLKYLAGIAGPSGYGSKSTADQVIEHSQF-SSSLTAIIT----GIGEETARVMAKRG 60
           MK TL+YLAGIAGP+G+GS+STA+QV +HS F  S LTAIIT    GIG ETARV+AKRG
Sbjct: 1   MKGTLRYLAGIAGPNGFGSRSTAEQVTQHSFFPCSHLTAIITGGTSGIGAETARVLAKRG 60

Query: 61  VRIVMAARDRKKAEEVKERIKRESPEAEILVTEIDLSSLASVRRFCVEFAALGLPLNILI 120
           VR+VMA RD KKAE VKERI RE+PEA+I++ EIDLSSL+SV RFC +F +  LPLNILI
Sbjct: 61  VRVVMAVRDMKKAEMVKERIIRENPEADIILFEIDLSSLSSVARFCSQFLSQDLPLNILI 120

Query: 121 NNGGVFSPNLEYSDDKIELTFATNYLGHYLLTEILLEKLIETSNKTGIQGRIINVSSMAH 180
           NN GVFSPNLE+S++KIELTFATN+LGHYLLTE+L+EK+I+T+ K+GI+GRIIN+SS+ H
Sbjct: 121 NNAGVFSPNLEFSEEKIELTFATNFLGHYLLTEMLIEKMIDTAEKSGIEGRIINLSSVIH 180

Query: 181 CWVKKDGLCFSHLLNP-NKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGI 240
            WVK D   F  LL+P ++YNGTRAYAQSKLA ILHAK +++QLK+R+ANVTINAVHPGI
Sbjct: 181 NWVKPDCFSFPKLLHPISRYNGTRAYAQSKLATILHAKALSKQLKDRNANVTINAVHPGI 240

Query: 241 VKTAIIRAHRGFITDSLFFMASKLLKSIPQ 265
           VKT IIRAH+G  TDSLF +ASKLLKSI Q
Sbjct: 241 VKTGIIRAHKGLFTDSLFLIASKLLKSISQ 270

BLAST of Sgr017624 vs. TAIR 10
Match: AT1G64590.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 329.7 bits (844), Expect = 2.6e-90
Identity = 179/321 (55.76%), Postives = 228/321 (71.03%), Query Frame = 0

Query: 1   MKETLKYLAGIAGPSGYGSKSTADQVIEHSQFSSSLTAIIT----GIGEETARVMAKRGV 60
           M ET+K+L G  GPSG+GS+STAD V  +S    SLTAIIT    GIG ETARV+AKRG 
Sbjct: 1   MIETVKHLIGSGGPSGFGSRSTADHVTCNSDL-RSLTAIITGATSGIGAETARVLAKRGA 60

Query: 61  RIVMAARDRKKAEEVKERIKRESPEAEILVTEIDLSSLASVRRFCVEFAALGLPLNILIN 120
           R+V+ AR  K AEE K RI  E P+AEI+V  +DLSSL SVRRF  +F +L LPLNILIN
Sbjct: 61  RLVLPARSVKTAEETKARILSEFPDAEIIVMHLDLSSLTSVRRFVDDFESLNLPLNILIN 120

Query: 121 NGGVFSPNLEYSDDKIELTFATNYLGHYLLTEILLEKLIETSNKTGIQGRIINVSSMAHC 180
           N G ++     S+D +E+TFATNYLGH+LLT++LL+K+IET+ +TG+QGRI+NV+S+ H 
Sbjct: 121 NAGKYAHKHALSEDGVEMTFATNYLGHFLLTKLLLKKMIETAAQTGVQGRIVNVTSVVHS 180

Query: 181 WVKKDGLCFSHLLNPN--KYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGI 240
           W   D L +   ++ N   Y+ TRAYA SKLA +LH  E++R L + DANVT N VHPGI
Sbjct: 181 WFSGDMLQYLADISRNNRNYDATRAYALSKLANVLHTVELSRLLHKMDANVTANCVHPGI 240

Query: 241 VKTAIIRAHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQTERKTGKYFADCNETKC 300
           VKT + R   G +TD +FF+ SKLLKS+PQ AATTCYVA SP+     GKYF+DCNE + 
Sbjct: 241 VKTRLTRDREGVVTDLVFFLTSKLLKSVPQAAATTCYVATSPRLRNVCGKYFSDCNEARS 300

Query: 301 SALANDKLVARKLWMQTRALV 316
           S   +  L A++LW  +  LV
Sbjct: 301 SKSGSCNLKAQRLWTASDLLV 320

BLAST of Sgr017624 vs. TAIR 10
Match: AT4G24050.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 323.9 bits (829), Expect = 1.4e-88
Identity = 172/321 (53.58%), Postives = 228/321 (71.03%), Query Frame = 0

Query: 1   MKETLKYLAGIAGPSGYGSKSTADQVIEHSQFSSSLTAIIT----GIGEETARVMAKRGV 60
           M ET KYL G AG SG+GSKSTA++V E+     S+TA+IT    GIG ETARV+AKRG 
Sbjct: 1   MIETGKYLLGAAGASGFGSKSTAEEVTENCDL-RSITAVITGATSGIGAETARVLAKRGA 60

Query: 61  RIVMAARDRKKAEEVKERIKRESPEAEILVTEIDLSSLASVRRFCVEFAALGLPLNILIN 120
           R++  AR+ K AEE KERI  E PE EI+V ++DLSS+ASVR F  +F +L LPLN+LIN
Sbjct: 61  RLIFPARNVKAAEEAKERIVSEFPETEIVVMKLDLSSIASVRNFVADFESLDLPLNLLIN 120

Query: 121 NGGVFSPNLEYSDDKIELTFATNYLGHYLLTEILLEKLIETSNKTGIQGRIINVSSMAHC 180
           N G  +     S+D IE+TFATNYLGH+LLT +LL K+I+T+ +TG+QGRI+NV+S  H 
Sbjct: 121 NAGKLAHEHAISEDGIEMTFATNYLGHFLLTNLLLNKMIQTAEETGVQGRIVNVTSGIHG 180

Query: 181 WVKKDGLCFSHLLNPNK--YNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGI 240
           W   D + +  L++  K  ++ TRAYA SKLA +LH KE++ +L++  ANVT+N VHPG+
Sbjct: 181 WFSGDLIEYLRLISQPKCQFDATRAYALSKLANVLHTKELSSRLQKIGANVTVNCVHPGV 240

Query: 241 VKTAIIRAHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQTERKTGKYFADCNETKC 300
           V+T + R   G +TD +FF+ASKL+K++PQ AATTCYVA +P+    +GKYF DCNET  
Sbjct: 241 VRTRLTRDREGLLTDLVFFLASKLVKTVPQAAATTCYVATNPRLVNVSGKYFTDCNETTP 300

Query: 301 SALANDKLVARKLWMQTRALV 316
           S L  +   A KLW  +  LV
Sbjct: 301 SGLGTNSSEATKLWAASEILV 320

BLAST of Sgr017624 vs. TAIR 10
Match: AT5G02540.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 277.7 bits (709), Expect = 1.2e-74
Identity = 149/305 (48.85%), Postives = 209/305 (68.52%), Query Frame = 0

Query: 8   LAGIAGPSGYGSKSTADQVIEHSQFSSSLTAIIT----GIGEETARVMAKRGVRIVMAAR 67
           + G  GPSG+GS STA++V +    +++LTAIIT    GIG ETARV++KRG  +V+ AR
Sbjct: 7   ITGRRGPSGFGSASTAEEVTQGID-ATNLTAIITGGTGGIGMETARVLSKRGAHVVIGAR 66

Query: 68  DRKKAEEVKERIKRESPEAEILVTEIDLSSLASVRRFCVEFAALGLPLNILINNGGVFSP 127
           +   AE  K  I R++  A + + ++DLSS+ S++ F  EF AL LPLN+LINN GV   
Sbjct: 67  NMGAAENAKTEILRQNANARVTLLQLDLSSIKSIKAFVREFHALHLPLNLLINNAGVMFC 126

Query: 128 NLEYSDDKIELTFATNYLGHYLLTEILLEKLIETSNKTGIQGRIINVSSMAHCWVKKDGL 187
             + S+D IEL FATN++GH+LLT +LL+ +  T+  +G++GRI+NVSS+AH +  ++G+
Sbjct: 127 PYQLSEDGIELQFATNHIGHFLLTNLLLDTMKNTAKTSGVEGRILNVSSVAHIYTYQEGI 186

Query: 188 CFSHLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVKTAIIRAH 247
            F  + +   Y+  RAY QSKLA ILHA E++RQL+E   N+T N+VHPG++ T + + H
Sbjct: 187 QFDSINDICSYSDKRAYGQSKLANILHANELSRQLQEEGVNITANSVHPGLILTNLFQ-H 246

Query: 248 RGFITDSLFFMASKLLKSIPQGAATTCYVALSPQTERKTGKYFADCNETKCSALANDKLV 307
              +   L F +  L K+IPQGAATTCYVAL P  +  TGKYFADCNE   S LA D+ +
Sbjct: 247 TALLMRFLKFFSFYLWKNIPQGAATTCYVALHPSVKGVTGKYFADCNEVTPSKLARDETL 306

Query: 308 ARKLW 309
           A+KLW
Sbjct: 307 AQKLW 309

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023000696.12.8e-15888.34short-chain dehydrogenase TIC 32, chloroplastic-like [Cucurbita maxima][more]
XP_023519347.18.9e-15787.12short-chain dehydrogenase TIC 32, chloroplastic-like [Cucurbita pepo subsp. pepo... [more]
XP_022924073.14.4e-15687.12short-chain dehydrogenase TIC 32, chloroplastic-like [Cucurbita moschata][more]
XP_038895224.13.2e-15486.81short-chain dehydrogenase TIC 32 A, chloroplastic-like isoform X2 [Benincasa his... [more]
XP_022137126.12.7e-15386.32short-chain dehydrogenase TIC 32, chloroplastic-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
A0A078IS662.7e-7148.85Short-chain dehydrogenase TIC 32 A, chloroplastic OS=Brassica napus OX=3708 GN=T... [more]
A0A078ISJ63.5e-7148.85Short-chain dehydrogenase TIC 32 B, chloroplastic OS=Brassica napus OX=3708 GN=T... [more]
A2RVM06.1e-6845.25Short-chain dehydrogenase TIC 32, chloroplastic OS=Arabidopsis thaliana OX=3702 ... [more]
Q6RVV41.6e-6343.67Short-chain dehydrogenase TIC 32, chloroplastic OS=Pisum sativum OX=3888 GN=TIC3... [more]
Q9HBH52.9e-4137.63Retinol dehydrogenase 14 OS=Homo sapiens OX=9606 GN=RDH14 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1KGJ51.3e-15888.34short-chain dehydrogenase TIC 32, chloroplastic-like OS=Cucurbita maxima OX=3661... [more]
A0A6J1EBB92.1e-15687.12short-chain dehydrogenase TIC 32, chloroplastic-like OS=Cucurbita moschata OX=36... [more]
A0A6J1C5L31.3e-15386.32short-chain dehydrogenase TIC 32, chloroplastic-like OS=Momordica charantia OX=3... [more]
A0A6J1E1M66.2e-13276.78short-chain dehydrogenase TIC 32, chloroplastic isoform X2 OS=Cucurbita moschata... [more]
A0A6J1JH352.4e-13176.47short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 OS=Cucurbita max... [more]
Match NameE-valueIdentityDescription
AT5G50130.12.6e-12270.64NAD(P)-binding Rossmann-fold superfamily protein [more]
AT5G50130.24.3e-10171.48NAD(P)-binding Rossmann-fold superfamily protein [more]
AT1G64590.12.6e-9055.76NAD(P)-binding Rossmann-fold superfamily protein [more]
AT4G24050.11.4e-8853.58NAD(P)-binding Rossmann-fold superfamily protein [more]
AT5G02540.11.2e-7448.85NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 58..78
NoneNo IPR availableGENE3D3.40.50.720coord: 34..319
e-value: 1.8E-66
score: 226.1
NoneNo IPR availablePIRSRPIRSR629511-2PIRSR629511-2coord: 37..240
e-value: 0.11
score: 9.3
NoneNo IPR availablePANTHERPTHR24320RETINOL DEHYDROGENASEcoord: 4..318
NoneNo IPR availablePANTHERPTHR24320:SF198NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEINcoord: 4..318
NoneNo IPR availableCDDcd05327retinol-DH_like_SDR_c_likecoord: 37..308
e-value: 2.28254E-95
score: 281.806
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00081GDHRDHcoord: 159..175
score: 32.64
coord: 200..219
score: 27.97
coord: 37..54
score: 27.13
coord: 223..240
score: 33.16
coord: 109..120
score: 39.38
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00080SDRFAMILYcoord: 165..173
score: 55.39
coord: 200..219
score: 26.08
coord: 109..120
score: 38.63
IPR002347Short-chain dehydrogenase/reductase SDRPFAMPF00106adh_shortcoord: 37..174
e-value: 7.2E-23
score: 81.1
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 37..293

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr017624.1Sgr017624.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016491 oxidoreductase activity