Sgr017609 (gene) Monk fruit (Qingpiguo) v1

Overview


Name	Sgr017609
Type	gene
Organism	Siraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description	cytochrome P450 71A1-like
Location	tig00153054: 86581 .. 87576 (+)
RNA-Seq Expression	Sgr017609
Synteny	Sgr017609

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATCCAACATCGTCAAATCTCCACCAATTTTGGCAACAACTTCAAGCTCACTTCAATCCCATCTCTTTTTCTCTCTGTCTTCTCTTCTTCTTTCTACTTTTGCTTGAGCTGTTCGGCGGCAAAAGGACCAACTCACCTCCTTCACCACCGCAACTTCCAATTATCGGCAACCTTCACCAAATAGGGAGCCTCCCGCACCGCTCTCTGGCAGCTCTTTCTGAGAAATATGGCCCTCTCATGCTCCTCAACTTGGGCCACACCCCAACTCTCGTGGTTTCGTCTGCAAAAATGGCTAGACAAGTCATGAAGTCTCACGACATCATATTCTCCAACAGGACCCAAAACACTGCTGCAAATCTCTTACTCTACGGGTGCCACGACGTCGCTTATGCTTCGTATGGAGAGTACTGGAGACAAGCCAGAAAAATTTGTGTTCTGGAGCTTTTAAGTGCCAAAAGAGTGAAGTTTTTTCAGCATGTGAGGGATGAAGAAGTTGCTGTGCTTATCAATAGGATTCGTGAAGCTTGTTGTGTTGATGGTTATGATTCTTCTGTGAATCTTAACCAGTTGTTGATGTCAACCTCAAACAGTATAGTCTCGAGATGCGTTCTGGGAGAAAAATTTGAGGATGAAAATGGAAACAGCAGATTTGGAGATGTAACAAGAAAGGTTATGGTGCTATTGACGGCGTTTTGTATCACTGACGCCTTCCCCGGTTTTCGATGGATCGATGTGTTGAGAGGATTCACCGGAGAACTCAAAAGCACTTTTAAAACACTGGACGCCTTCTTTGATAAGGTGATTGAAGAACACATGGAAAAGATGAGTAGTGATGATCAACCAGATCATGAGAAAGACTTCGTGGATGTCATGCACCAACTGCAACAAGACGACACACTCGACTATCGTTTCACTCGGGACAATTTGAAAGCAATTTTACTGGTTTCTCTCTCTCTCTCTCTCTCTCTCTCTTACATGCACTCACACACGTAG

mRNA sequence

ATGGATCCAACATCGTCAAATCTCCACCAATTTTGGCAACAACTTCAAGCTCACTTCAATCCCATCTCTTTTTCTCTCTGTCTTCTCTTCTTCTTTCTACTTTTGCTTGAGCTGTTCGGCGGCAAAAGGACCAACTCACCTCCTTCACCACCGCAACTTCCAATTATCGGCAACCTTCACCAAATAGGGAGCCTCCCGCACCGCTCTCTGGCAGCTCTTTCTGAGAAATATGGCCCTCTCATGCTCCTCAACTTGGGCCACACCCCAACTCTCGTGGTTTCGTCTGCAAAAATGGCTAGACAAGTCATGAAGTCTCACGACATCATATTCTCCAACAGGACCCAAAACACTGCTGCAAATCTCTTACTCTACGGGTGCCACGACGTCGCTTATGCTTCGTATGGAGAGTACTGGAGACAAGCCAGAAAAATTTGTGTTCTGGAGCTTTTAAGTGCCAAAAGAGTGAAGTTTTTTCAGCATGTGAGGGATGAAGAAGTTGCTGTGCTTATCAATAGGATTCGTGAAGCTTGTTGTGTTGATGGTTATGATTCTTCTGTGAATCTTAACCAGTTGTTGATGTCAACCTCAAACAGTATAGTCTCGAGATGCGTTCTGGGAGAAAAATTTGAGGATGAAAATGGAAACAGCAGATTTGGAGATGTAACAAGAAAGGTTATGGTGCTATTGACGGCGTTTTGTATCACTGACGCCTTCCCCGGTTTTCGATGGATCGATGTGTTGAGAGGATTCACCGGAGAACTCAAAAGCACTTTTAAAACACTGGACGCCTTCTTTGATAAGGTGATTGAAGAACACATGGAAAAGATGAGTAGTGATGATCAACCAGATCATGAGAAAGACTTCGTGGATGTCATGCACCAACTGCAACAAGACGACACACTCGACTATCGTTTCACTCGGGACAATTTGAAAGCAATTTTACTGGTTTCTCTCTCTCTCTCTCTCTCTCTCTCTTACATGCACTCACACACGTAG

Coding sequence (CDS)

ATGGATCCAACATCGTCAAATCTCCACCAATTTTGGCAACAACTTCAAGCTCACTTCAATCCCATCTCTTTTTCTCTCTGTCTTCTCTTCTTCTTTCTACTTTTGCTTGAGCTGTTCGGCGGCAAAAGGACCAACTCACCTCCTTCACCACCGCAACTTCCAATTATCGGCAACCTTCACCAAATAGGGAGCCTCCCGCACCGCTCTCTGGCAGCTCTTTCTGAGAAATATGGCCCTCTCATGCTCCTCAACTTGGGCCACACCCCAACTCTCGTGGTTTCGTCTGCAAAAATGGCTAGACAAGTCATGAAGTCTCACGACATCATATTCTCCAACAGGACCCAAAACACTGCTGCAAATCTCTTACTCTACGGGTGCCACGACGTCGCTTATGCTTCGTATGGAGAGTACTGGAGACAAGCCAGAAAAATTTGTGTTCTGGAGCTTTTAAGTGCCAAAAGAGTGAAGTTTTTTCAGCATGTGAGGGATGAAGAAGTTGCTGTGCTTATCAATAGGATTCGTGAAGCTTGTTGTGTTGATGGTTATGATTCTTCTGTGAATCTTAACCAGTTGTTGATGTCAACCTCAAACAGTATAGTCTCGAGATGCGTTCTGGGAGAAAAATTTGAGGATGAAAATGGAAACAGCAGATTTGGAGATGTAACAAGAAAGGTTATGGTGCTATTGACGGCGTTTTGTATCACTGACGCCTTCCCCGGTTTTCGATGGATCGATGTGTTGAGAGGATTCACCGGAGAACTCAAAAGCACTTTTAAAACACTGGACGCCTTCTTTGATAAGGTGATTGAAGAACACATGGAAAAGATGAGTAGTGATGATCAACCAGATCATGAGAAAGACTTCGTGGATGTCATGCACCAACTGCAACAAGACGACACACTCGACTATCGTTTCACTCGGGACAATTTGAAAGCAATTTTACTGGTTTCTCTCTCTCTCTCTCTCTCTCTCTCTTACATGCACTCACACACGTAG

Protein sequence

MDPTSSNLHQFWQQLQAHFNPISFSLCLLFFFLLLLELFGGKRTNSPPSPPQLPIIGNLHQIGSLPHRSLAALSEKYGPLMLLNLGHTPTLVVSSAKMARQVMKSHDIIFSNRTQNTAANLLLYGCHDVAYASYGEYWRQARKICVLELLSAKRVKFFQHVRDEEVAVLINRIREACCVDGYDSSVNLNQLLMSTSNSIVSRCVLGEKFEDENGNSRFGDVTRKVMVLLTAFCITDAFPGFRWIDVLRGFTGELKSTFKTLDAFFDKVIEEHMEKMSSDDQPDHEKDFVDVMHQLQQDDTLDYRFTRDNLKAILLVSLSLSLSLSYMHSHT

Homology

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BLAST of Sgr017609 vs. NCBI nr
Match: XP_022137028.1 (cytochrome P450 71A1-like [Momordica charantia])

HSP 1 Score: 439.9 bits (1130), Expect = 2.0e-119
Identity = 227/326 (69.63%), Postives = 259/326 (79.45%), Query Frame = 0


Query: 1   MDPTSSNLHQFWQQL-------QAHF-NPISFSL--CLLFFFLLLLELFGGKRTN-SPPS 60
           MDP SSNLHQ WQQL         HF NPISF L   L  FFL+LL+LF  K  N  PPS
Sbjct: 14  MDPKSSNLHQIWQQLVEGPYGATIHFNNPISFPLIFLLFLFFLILLKLFRAKNINLLPPS 73

Query: 61  PPQLPIIGNLHQIGSLPHRSLAALSEKYGPLMLLNLGHTPTLVVSSAKMARQVMKSHDII 120
           PPQLPIIGNLHQIG+LPH SLA LS KYGP+M L LGH PTL++SSAK+AR+VMKS DII
Sbjct: 74  PPQLPIIGNLHQIGALPHLSLATLSNKYGPIMFLKLGHAPTLIISSAKLAREVMKSQDII 133

Query: 121 FSNRTQNTAANLLLYGCHDVAYASYGEYWRQARKICVLELLSAKRVKFFQHVRDEEVAVL 180
           F NR QNTAAN LLYGCHD+A+ S GE WRQA+KICV+ELLSAKRV  FQHVRDEEVA+L
Sbjct: 134 FCNRAQNTAANHLLYGCHDIAFGSCGELWRQAKKICVVELLSAKRVDSFQHVRDEEVAIL 193

Query: 181 INRIREACCVDGYDSSVNLNQLLMSTSNSIVSRCVLGEKFEDENGNSRFGDVTRKVMVLL 240
           I RIR++C      SS NLN+LL++T N+I+SRCVLGEKFEDENG+S+FG VTRK+M LL
Sbjct: 194 IKRIRKSC---DSSSSTNLNKLLLATINNIISRCVLGEKFEDENGDSKFGGVTRKIMELL 253

Query: 241 TAFCITDAFPGFRWIDVLRGFTGELKSTFKTLDAFFDKVIEEHMEKMSSDDQPDHEKDFV 300
           T FC+ D FPGF  IDV+RGF GEL++TFKT+DAFFDKVIE+H EKM   D    E+DFV
Sbjct: 254 TRFCVADFFPGFGLIDVVRGFIGELETTFKTMDAFFDKVIEKHREKMGESDDKSDEQDFV 313

Query: 301 DVMHQLQQDDTLDYRFTRDNLKAILL 316
           D+M QLQQ+ T DYRFTRDNLKAILL
Sbjct: 314 DIMLQLQQNKTSDYRFTRDNLKAILL 336

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BLAST of Sgr017609 vs. NCBI nr
Match: XP_022137055.1 (cytochrome P450 71A1-like [Momordica charantia])

HSP 1 Score: 410.6 bits (1054), Expect = 1.3e-110
Identity = 209/313 (66.77%), Postives = 245/313 (78.27%), Query Frame = 0


Query: 6   SNLHQFWQQLQAHFNPISFSLCLLFFFLLLLELFGGKRT--NSPPSPPQLPIIGNLHQIG 65
           SN  QFWQQL  HF+ I FSLCL+FFFL+LL+LF  K    N PPSPP+LPIIGNLHQ+G
Sbjct: 5   SNFLQFWQQL--HFDLIPFSLCLIFFFLVLLKLFKPKNNTKNFPPSPPKLPIIGNLHQLG 64

Query: 66  SLPHRSLAALSEKYGPLMLLNLGHTPTLVVSSAKMARQVMKSHDIIFSNRTQNTAANLLL 125
           SLPH+SLAALS+KYGPLMLL LGH  TLVVSSAKMAR++MK+HD+ FSNR Q   A  LL
Sbjct: 65  SLPHQSLAALSDKYGPLMLLKLGHAQTLVVSSAKMARELMKTHDLKFSNRPQTMPAKTLL 124

Query: 126 YGCHDVAYASYGEYWRQARKICVLELLSAKRVKFFQHVRDEEVAVLINRIREACCVDGYD 185
           YG  DV +A YGEYWRQARKIC LEL SAKRV+ FQ+VRDEEV+VL+NRIR+ C     +
Sbjct: 125 YGFKDVGFAPYGEYWRQARKICALELFSAKRVESFQYVRDEEVSVLVNRIRKGCVDGDGE 184

Query: 186 SSVNLNQLLMSTSNSIVSRCVLGEKFEDENGNSRFGDVTRKVMVLLTAFCITDAFPGFRW 245
            SVNL+QL +STSN+IVSRC+LGEKFEDENG SRFGD +R+VMVL+TAFC  D FP   W
Sbjct: 185 GSVNLSQLFLSTSNNIVSRCILGEKFEDENGKSRFGDTSRRVMVLITAFCFADFFPALWW 244

Query: 246 IDVLRGFTGELKSTFKTLDAFFDKVIEEHMEKMSSDDQPDH-EKDFVDVMHQLQQDDTLD 305
           IDV+RGF GELK  F TLDAF  KV+EEH  KM +  + D  +KDFV +M QL Q D +D
Sbjct: 245 IDVVRGFNGELKKWFTTLDAFLSKVVEEHKAKMRAGGESDESKKDFVGIMLQLMQSDMVD 304

Query: 306 YRFTRDNLKAILL 316
           Y F+ +NLK I+L
Sbjct: 305 YHFSLENLKGIVL 315

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BLAST of Sgr017609 vs. NCBI nr
Match: XP_023001252.1 (cytochrome P450 71A1-like [Cucurbita maxima])

HSP 1 Score: 403.3 bits (1035), Expect = 2.0e-108
Identity = 214/318 (67.30%), Postives = 251/318 (78.93%), Query Frame = 0


Query: 1   MDPTS-SNLHQFWQQLQAHFNPISFSLCLLFFFLLLLELFGGKRTNS-PPSPPQLPIIGN 60
           MDP S  N+HQ   Q+     P+ F    LF FLL L+LF  K T + PPSPPQLPIIGN
Sbjct: 1   MDPISYKNVHQLLHQITQAPLPL-FLFLFLFLFLLSLKLFSTKSTKALPPSPPQLPIIGN 60

Query: 61  LHQIGSLPHRSLAALSEKYGPLMLLNLGHTPTLVVSSAKMARQVMKSHDIIFSNRTQNTA 120
           LHQ+GSLPHRS+A+LSEKYGPLMLL LG TPTLVVSSAK+AR+VM+SHD IFSNR QN A
Sbjct: 61  LHQLGSLPHRSVASLSEKYGPLMLLKLGQTPTLVVSSAKLAREVMRSHDTIFSNRYQNKA 120

Query: 121 ANLLLYGCHDVAYASYGEYWRQARKICVLELLSAKRVKFFQHVRDEEVAVLINRIREACC 180
           A  L+YGCHDVA+ASYGE WRQARK+CVLELLSAKRV+ FQ+VRDEEV  L+ +IR+ C 
Sbjct: 121 AQSLIYGCHDVAFASYGEQWRQARKLCVLELLSAKRVECFQYVRDEEVGELVKKIRKGC- 180

Query: 181 VDGY-DSSVNLNQLLMSTSNSIVSRCVLGEKFEDENGNSRFGDVTRKVMVLLTAFCITDA 240
             G+ DS VNLNQ+LMSTSN+IVSRCVLGEK E+  G S FGDVTRKVMVLL+AFC+ DA
Sbjct: 181 --GFGDSGVNLNQVLMSTSNNIVSRCVLGEKLENGKGMSGFGDVTRKVMVLLSAFCVGDA 240

Query: 241 FPGFRWIDVLRGFTGELKSTFKTLDAFFDKVIEEHMEKMSSDDQPDHEKDFVDVMHQLQQ 300
            P   W+DV+RGF  ELK+TF+ LD  FD+VI+EH EKM + D+   E DFVD++ QLQQ
Sbjct: 241 LPWLGWVDVVRGFHRELKNTFEALDGLFDRVIDEHREKMKNSDR-SGEMDFVDIILQLQQ 300

Query: 301 DDTLDYRFTRDNLKAILL 316
            DTLDYRFT ++ KAILL
Sbjct: 301 RDTLDYRFTMNDYKAILL 313

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BLAST of Sgr017609 vs. NCBI nr
Match: XP_023520090.1 (cytochrome P450 71A1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 401.0 bits (1029), Expect = 1.0e-107
Identity = 213/318 (66.98%), Postives = 250/318 (78.62%), Query Frame = 0


Query: 1   MDPTS-SNLHQFWQQLQAHFNPISFSLCLLFFFLLLLELFGGKRTNS-PPSPPQLPIIGN 60
           MDP S  N+HQ  QQ+     P+ F    LF FLL L+LF  K T + PPSPPQLPIIGN
Sbjct: 1   MDPISHKNVHQLLQQITQAPLPL-FLFLFLFLFLLSLKLFSTKSTKALPPSPPQLPIIGN 60

Query: 61  LHQIGSLPHRSLAALSEKYGPLMLLNLGHTPTLVVSSAKMARQVMKSHDIIFSNRTQNTA 120
           LHQ+GSLPHRS+A+LS+KYGPLMLL LG  PTLVVSSAK+AR+VMKSHD IFSNR QN A
Sbjct: 61  LHQLGSLPHRSVASLSDKYGPLMLLKLGQIPTLVVSSAKLAREVMKSHDTIFSNRYQNRA 120

Query: 121 ANLLLYGCHDVAYASYGEYWRQARKICVLELLSAKRVKFFQHVRDEEVAVLINRIREACC 180
           A  L+YGC DVA+ASYGE WRQARK+CVLELLSAKRV+ FQ+VRDEEV  L+ +IR+ C 
Sbjct: 121 AQSLIYGCRDVAFASYGEQWRQARKLCVLELLSAKRVESFQYVRDEEVGELVKKIRKGC- 180

Query: 181 VDGY-DSSVNLNQLLMSTSNSIVSRCVLGEKFEDENGNSRFGDVTRKVMVLLTAFCITDA 240
             G+ DS VNLNQLLMSTSN+IVSRCVLGEKFE+  G S FGDVTRKVMVLL+AFC+ DA
Sbjct: 181 --GFGDSGVNLNQLLMSTSNNIVSRCVLGEKFENGKGMSGFGDVTRKVMVLLSAFCVGDA 240

Query: 241 FPGFRWIDVLRGFTGELKSTFKTLDAFFDKVIEEHMEKMSSDDQPDHEKDFVDVMHQLQQ 300
            P   W+D +RGF  ELK+TF+ LD  FD+VI+EH EKM + D+   E DFVD++ QLQQ
Sbjct: 241 LPWLGWVDAVRGFNRELKNTFEALDGLFDRVIDEHREKMKNSDR-SGEMDFVDIILQLQQ 300

Query: 301 DDTLDYRFTRDNLKAILL 316
            DTLDYRF+ ++ KAILL
Sbjct: 301 RDTLDYRFSMNDYKAILL 313

[Close]

BLAST of Sgr017609 vs. NCBI nr
Match: XP_022137054.1 (cytochrome P450 71A1-like [Momordica charantia])

HSP 1 Score: 399.1 bits (1024), Expect = 3.8e-107
Identity = 208/320 (65.00%), Postives = 246/320 (76.88%), Query Frame = 0


Query: 1   MDPTSSNLHQFWQQLQAHFNPISFSLCLLFFFLLLLELFGGKRT--NSPPSPPQLPIIGN 60
           MDP  S   Q WQQ    F+ I FSLC+ FFFL+LL+L   K    N PPSPP+LPIIGN
Sbjct: 1   MDPI-SKFEQIWQQ----FDLIPFSLCIFFFFLVLLKLLKPKNNTKNFPPSPPKLPIIGN 60

Query: 61  LHQIGSLPHRSLAALSEKYGPLMLLNLGHTPTLVVSSAKMARQVMKSHDIIFSNRTQNTA 120
           LHQ+GSLPH+SLAALS+K+GPLM L LG  PTLVVSSA+MAR+VMK+HD+ FS+R Q TA
Sbjct: 61  LHQLGSLPHQSLAALSDKHGPLMFLKLGQAPTLVVSSAEMAREVMKTHDLNFSDRPQTTA 120

Query: 121 ANLLLYGCHDVAYASYGEYWRQARKICVLELLSAKRVKFFQHVRDEEVAVLINRIREACC 180
           A +LLYG  DV +A YGEYWRQARKIC LEL SAKRV+ FQ+VRDEEVAVL+NRIR  C 
Sbjct: 121 AKILLYGFKDVGFAPYGEYWRQARKICALELFSAKRVESFQYVRDEEVAVLVNRIRRGCV 180

Query: 181 VDGYDSSVNLNQLLMSTSNSIVSRCVLGEKFEDENGNSRFGDVTRKVMVLLTAFCITDAF 240
               + SVNL QL +STSN+IVSRC++G+KFEDENG SRFGD++R+VMVLLTAFC  D F
Sbjct: 181 GGDGEGSVNLAQLFLSTSNNIVSRCMMGDKFEDENGKSRFGDISRRVMVLLTAFCFADFF 240

Query: 241 PGFRWIDVLRGFTGELKSTFKTLDAFFDKVIEEHMEKMSSDDQ--PDHEKDFVDVMHQL- 300
           P   WIDV+RGF GELK  F TLDAFF KV+EEH  KM    +   + ++DF+D M QL 
Sbjct: 241 PALWWIDVVRGFNGELKKCFTTLDAFFSKVVEEHKAKMIGGGELCDESKRDFMDSMLQLI 300

Query: 301 QQDDTLDYRFTRDNLKAILL 316
            QDD +DYRF+ DNLKAI+L
Sbjct: 301 LQDDMVDYRFSLDNLKAIVL 315

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BLAST of Sgr017609 vs. ExPASy Swiss-Prot
Match: A0A068Q609 (Phenylacetaldehyde oxime monooxygenase CYP71AN24 OS=Prunus mume OX=102107 GN=CYP71AN24 PE=1 SV=1)

HSP 1 Score: 289.7 bits (740), Expect = 4.3e-77
Identity = 152/317 (47.95%), Postives = 209/317 (65.93%), Query Frame = 0


Query: 9   HQFWQQLQAHFNPISFS------LCLLFFFLLLLELFG-----GKRTNSPPSPPQLPIIG 68
           +Q WQ+ Q   +  SF+      LCL  F L  L          ++   PPSPP+LP IG
Sbjct: 8   NQIWQEGQLQSSTSSFNIFLVPILCLSIFILFSLTRSSSPSEKNRKLKLPPSPPRLPWIG 67

Query: 69  NLHQIGSLPHRSLAALSEKYGPLMLLNLGHTPTLVVSSAKMARQVMKSHDIIFSNRTQNT 128
           NLHQ+GS PHRSL ALS+KYG +M ++ G  PTL+VSSA+MA+ VMK+ DI+F +R Q T
Sbjct: 68  NLHQLGSFPHRSLRALSKKYGDVMFMHFGKVPTLIVSSAEMAKDVMKTQDIVFCSRPQTT 127

Query: 129 AANLLLYGCHDVAYASYGEYWRQARKICVLELLSAKRVKFFQHVRDEEVAVLINRIREAC 188
           A ++L Y  HD+A+A YGEYWRQ R+ICVLELLS KRV  FQ+ R EEVA L+++IR+A 
Sbjct: 128 APSILFYDGHDIAFAPYGEYWRQVRRICVLELLSLKRVHQFQYARVEEVAELVSKIRKA- 187

Query: 189 CVDGYDSSVNLNQLLMSTSNSIVSRCVLGEKFEDENGNSRFGDVTRKVMVLLTAFCITDA 248
                 + +NL +LL+STSN+I+ RC+LG+KFED+  N  FG+ T+++M  + +F   D 
Sbjct: 188 SASANGAPINLGELLVSTSNNIICRCILGQKFEDKEDN-WFGETTKELMTQVMSFSFGDF 247

Query: 249 FPGFRWIDVLRGFTGELKSTFKTLDAFFDKVIEEHMEKMSSDDQPDHEKDFVDVMHQLQQ 308
           FP  +WID  RG+   LKS +   D FFDK+I+EH  K +  +    +KD VD++  +Q 
Sbjct: 248 FPSLKWIDRARGYLAYLKSIWLEFDKFFDKLIDEH--KAAQKEGKPRKKDIVDILLDVQN 307

Query: 309 DDTLDYRFTRDNLKAIL 315
           D +LD+  T  N+KAIL
Sbjct: 308 DGSLDFELTTSNVKAIL 320

[Close]

BLAST of Sgr017609 vs. ExPASy Swiss-Prot
Match: P24465 (Cytochrome P450 71A1 OS=Persea americana OX=3435 GN=CYP71A1 PE=1 SV=2)

HSP 1 Score: 274.6 bits (701), Expect = 1.4e-72
Identity = 137/294 (46.60%), Postives = 199/294 (67.69%), Query Frame = 0


Query: 24  FSLCLLFFFLLLLELFGGKRTNSPPSPPQLPIIGNLHQIGSLPHRSLAALSEKYGPLMLL 83
           F    L FFLL L     K+ N PPSPP LPIIGNLHQ+G+LPHRSL +L+ + GPL+LL
Sbjct: 9   FLAIALTFFLLKLNEKREKKPNLPPSPPNLPIIGNLHQLGNLPHRSLRSLANELGPLILL 68

Query: 84  NLGHTPTLVVSSAKMARQVMKSHDIIFSNRTQNTAANLLLYGCHDVAYASYGEYWRQARK 143
           +LGH PTL+VS+A++A +++K+HD+IF++R   TAA  + Y C DVA++ YGEYWRQ RK
Sbjct: 69  HLGHIPTLIVSTAEIAEEILKTHDLIFASRPSTTAARRIFYDCTDVAFSPYGEYWRQVRK 128

Query: 144 ICVLELLSAKRVKFFQHVRDEEVAVLINRIREACCVDGYDSSVNLNQLLMSTSNSIVSRC 203
           ICVLELLS KRV  ++ +R+EEV +++ RI ++C       +VNL++LL+  S+  ++R 
Sbjct: 129 ICVLELLSIKRVNSYRSIREEEVGLMMERISQSCST---GEAVNLSELLLLLSSGTITRV 188

Query: 204 VLGEKFE-DENGNSRFGDVTRKVMVLLTAFCITDAFPGFRWIDVLRGFTGELKSTFKTLD 263
             G+K+E +E   ++F D+  ++  L+ AF + D FP F W+DVL G    LK     LD
Sbjct: 189 AFGKKYEGEEERKNKFADLATELTTLMGAFFVGDYFPSFAWVDVLTGMDARLKRNHGELD 248

Query: 264 AFFDKVIEEH-MEKMSSDDQPDHEKDFVDVMHQLQQDDTLDYRFTRDNLKAILL 316
           AF D VI++H + + ++      +KD VDV+  LQ+D +L     R+NLKA++L
Sbjct: 249 AFVDHVIDDHLLSRKANGSDGVEQKDLVDVLLHLQKDSSLGVHLNRNNLKAVIL 299

[Close]

BLAST of Sgr017609 vs. ExPASy Swiss-Prot
Match: A0A068Q721 (Cytochrome P450 71AP13 OS=Prunus mume OX=102107 GN=CYP71AP13 PE=2 SV=1)

HSP 1 Score: 240.0 bits (611), Expect = 3.9e-62
Identity = 126/305 (41.31%), Postives = 184/305 (60.33%), Query Frame = 0


Query: 13  QQLQAHFNPISFSLCLLFFFLLLLELFGGKRTNSPPSPPQLPIIGNLHQIGSLPHRSLAA 72
           Q L+ H +  +FSL +L    +  +    +R   PPSPP+LP+IGNLHQ+G+ PH SL  
Sbjct: 15  QLLKEHSSLFAFSLLILLLKFIYKDKSRKRRVKLPPSPPKLPVIGNLHQLGNKPHLSLRC 74

Query: 73  LSEKYGPLMLLNLGHTPTLVVSSAKMARQVMKSHDIIFSNRTQNTAANLLLYGCHDVAYA 132
           L+EKYGP++ L LG  PT+VVSSA++A++V+K+HD+  S+R Q  +A  L Y C DV ++
Sbjct: 75  LAEKYGPIIYLQLGEIPTVVVSSARLAKEVLKTHDLALSSRPQIFSAKHLFYNCTDVVFS 134

Query: 133 SYGEYWRQARKICVLELLSAKRVKFFQHVRDEEVAVLINRIREACCVDGYDSSVNLNQLL 192
            YG YWR  RKIC+LELLSAKRV+ F HVR+EEVA L+ R+ E      Y  + NL ++L
Sbjct: 135 PYGAYWRHIRKICILELLSAKRVQSFSHVREEEVARLVRRVAEF-----YPGTTNLTKML 194

Query: 193 MSTSNSIVSRCVLGEKFEDENGNSRFG--DVTRKVMVLLTAFCITDAFPGFRWIDVLRGF 252
              +N ++ R   G  F +     R G   +  +   LL  F I D FP   +I  L G 
Sbjct: 195 GLYANDVLCRVAFGRGFSEGGDYDRHGFQKMLEEYQELLGGFSIGDFFPSMEFIHSLTGM 254

Query: 253 TGELKSTFKTLDAFFDKVIEEHMEKMSSDDQPDHEKDFVDVMHQLQQDDTLDYRFTRDNL 312
              L+ TF+  D  FD+++ +H   +S   + +  KD VDV+  +Q+ ++ +   T DN+
Sbjct: 255 KSRLQETFRRFDELFDQMVTDH---LSPKREKEEHKDLVDVLLDIQKKESTEMPLTMDNV 311

Query: 313 KAILL 316
           KAI+L
Sbjct: 315 KAIIL 311

[Close]

BLAST of Sgr017609 vs. ExPASy Swiss-Prot
Match: Q9STL1 (Cytochrome P450 71A22 OS=Arabidopsis thaliana OX=3702 GN=CYP71A22 PE=2 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 3.7e-60
Identity = 119/294 (40.48%), Postives = 188/294 (63.95%), Query Frame = 0


Query: 22  ISFSLCLLFFFLLLLELFGGKRTNSPPSPPQLPIIGNLHQIGSLPHRSLAALSEKYGPLM 81
           I  SL +    L  ++   GK++N+P SPP+LP+IGNLHQ+G  PHRSL +LS +YGPLM
Sbjct: 8   ILLSLIIFITILFFIKQKKGKKSNTPASPPRLPLIGNLHQLGRHPHRSLCSLSNRYGPLM 67

Query: 82  LLNLGHTPTLVVSSAKMARQVMKSHDIIFSNRTQNTAANLLLYGCHDVAYASYGEYWRQA 141
           LL  G  P LVVSSA +AR ++K++D +F++R ++     + Y   DVA A YGEYWRQ 
Sbjct: 68  LLRFGLVPVLVVSSADVARDILKTYDRVFASRPRSKIFEKIFYEARDVALAPYGEYWRQM 127

Query: 142 RKICVLELLSAKRVKFFQHVRDEEVAVLINRIREACCVDGYDSSVNLNQLLMSTSNSIVS 201
           + +CVL LL+ K V+ F++VR EE+++++ +I+++  +      VNL++LL S +N ++S
Sbjct: 128 KSVCVLHLLTNKMVRSFRNVRQEEISLMMEKIQKSSSL-----QVNLSELLGSLTNDVIS 187

Query: 202 RCVLGEKFEDENGNSRFGDVTRKVMVLLTAFCITDAFPGFRWIDVLRGFTGELKSTFKTL 261
           R  LG K+ DE   + F ++ +++  LL  FC+    P   WID + G  G+LK T   L
Sbjct: 188 RVALGRKYSDE---TDFKELMKRLTKLLGEFCVGTYVPWLAWIDWISGLDGQLKKTGNDL 247

Query: 262 DAFFDKVIEEHMEKMSSDDQPDHEKDFVDVMHQLQQDDTLDYRFTRDNLKAILL 316
           D F +KV+++H      +D      DFVDV+ ++Q++ ++ +   R ++KAI+L
Sbjct: 248 DEFLEKVVQDH------EDGDAQRTDFVDVLLRIQREKSVGFEIDRLSIKAIIL 287

[Close]

BLAST of Sgr017609 vs. ExPASy Swiss-Prot
Match: P37117 (Cytochrome P450 71A4 OS=Solanum melongena OX=4111 GN=CYP71A4 PE=2 SV=1)

HSP 1 Score: 233.0 bits (593), Expect = 4.8e-60
Identity = 127/306 (41.50%), Postives = 184/306 (60.13%), Query Frame = 0


Query: 26  LCLLFFFLLLLELFGGKRTNS---PPSPPQLPIIGNLHQIGSLPHRSLAALSEKYGPLML 85
           L LLF FLL+   F   +T +   PPSP +LPIIGNLHQ+GS PHRSL  LS+KYGP+ML
Sbjct: 13  LLLLFIFLLIHHCFTTSKTQNMFLPPSPRKLPIIGNLHQLGSHPHRSLRKLSQKYGPVML 72

Query: 86  LNLGHTPTLVVSSAKMARQVMKSHDIIFSNRTQNTAANLLLYGCHDVAYASYGEYWRQAR 145
           L+LG  P +V SS   AR ++K+HD +++ R + + A+ LLYG  DV ++ +GEYW Q R
Sbjct: 73  LHLGSKPVIVASSVDAARDILKTHDHVWATRPKYSIADSLLYGSKDVGFSPFGEYWWQVR 132

Query: 146 KICVLELLSAKRVKFFQHVRDEEVAVLINRIREACCVDGYDSSVNLNQLLMSTSNSIVSR 205
            I VL LLS KRV+ ++ VR+EE A +I +IR+ C      S +NL + L   +N+I SR
Sbjct: 133 SIVVLHLLSNKRVQSYRDVREEETANMIEKIRQGCDA----SVINLGEHLCFLTNNITSR 192

Query: 206 CVLGEKFEDENGNSRFGDVTRKVMVLLTAFCITDAFPGFRWIDVLRGFTGELKSTFKTLD 265
             LG  +++        D+  + + LL  F + D  P  +W++ + G   +++   K LD
Sbjct: 193 VALGRTYDERESGIDAKDILEQFLQLLDTFNVGDYIPWLKWVNKITGLDTKVEKIAKKLD 252

Query: 266 AFFDKVIEEHMEKMSSDDQ--PDHEKDFVDVMHQLQQDDTLDYRFTRDNLKAILLVSLSL 325
            F D VIEEH+ +   ++    D  KDFVDV+ ++Q     D+   RD+LKAILL + + 
Sbjct: 253 TFLDSVIEEHIIRNKKEEYAITDEAKDFVDVLLEIQNGKETDFPLQRDSLKAILLDAFAA 312

Query: 326 SLSLSY 327
                Y
Sbjct: 313 GTDTIY 314

[Close]

BLAST of Sgr017609 vs. ExPASy TrEMBL
Match: A0A6J1C953 (cytochrome P450 71A1-like OS=Momordica charantia OX=3673 GN=LOC111008589 PE=3 SV=1)

HSP 1 Score: 439.9 bits (1130), Expect = 9.5e-120
Identity = 227/326 (69.63%), Postives = 259/326 (79.45%), Query Frame = 0


Query: 1   MDPTSSNLHQFWQQL-------QAHF-NPISFSL--CLLFFFLLLLELFGGKRTN-SPPS 60
           MDP SSNLHQ WQQL         HF NPISF L   L  FFL+LL+LF  K  N  PPS
Sbjct: 14  MDPKSSNLHQIWQQLVEGPYGATIHFNNPISFPLIFLLFLFFLILLKLFRAKNINLLPPS 73

Query: 61  PPQLPIIGNLHQIGSLPHRSLAALSEKYGPLMLLNLGHTPTLVVSSAKMARQVMKSHDII 120
           PPQLPIIGNLHQIG+LPH SLA LS KYGP+M L LGH PTL++SSAK+AR+VMKS DII
Sbjct: 74  PPQLPIIGNLHQIGALPHLSLATLSNKYGPIMFLKLGHAPTLIISSAKLAREVMKSQDII 133

Query: 121 FSNRTQNTAANLLLYGCHDVAYASYGEYWRQARKICVLELLSAKRVKFFQHVRDEEVAVL 180
           F NR QNTAAN LLYGCHD+A+ S GE WRQA+KICV+ELLSAKRV  FQHVRDEEVA+L
Sbjct: 134 FCNRAQNTAANHLLYGCHDIAFGSCGELWRQAKKICVVELLSAKRVDSFQHVRDEEVAIL 193

Query: 181 INRIREACCVDGYDSSVNLNQLLMSTSNSIVSRCVLGEKFEDENGNSRFGDVTRKVMVLL 240
           I RIR++C      SS NLN+LL++T N+I+SRCVLGEKFEDENG+S+FG VTRK+M LL
Sbjct: 194 IKRIRKSC---DSSSSTNLNKLLLATINNIISRCVLGEKFEDENGDSKFGGVTRKIMELL 253

Query: 241 TAFCITDAFPGFRWIDVLRGFTGELKSTFKTLDAFFDKVIEEHMEKMSSDDQPDHEKDFV 300
           T FC+ D FPGF  IDV+RGF GEL++TFKT+DAFFDKVIE+H EKM   D    E+DFV
Sbjct: 254 TRFCVADFFPGFGLIDVVRGFIGELETTFKTMDAFFDKVIEKHREKMGESDDKSDEQDFV 313

Query: 301 DVMHQLQQDDTLDYRFTRDNLKAILL 316
           D+M QLQQ+ T DYRFTRDNLKAILL
Sbjct: 314 DIMLQLQQNKTSDYRFTRDNLKAILL 336

[Close]

BLAST of Sgr017609 vs. ExPASy TrEMBL
Match: A0A6J1C5L5 (cytochrome P450 71A1-like OS=Momordica charantia OX=3673 GN=LOC111008617 PE=3 SV=1)

HSP 1 Score: 410.6 bits (1054), Expect = 6.2e-111
Identity = 209/313 (66.77%), Postives = 245/313 (78.27%), Query Frame = 0


Query: 6   SNLHQFWQQLQAHFNPISFSLCLLFFFLLLLELFGGKRT--NSPPSPPQLPIIGNLHQIG 65
           SN  QFWQQL  HF+ I FSLCL+FFFL+LL+LF  K    N PPSPP+LPIIGNLHQ+G
Sbjct: 5   SNFLQFWQQL--HFDLIPFSLCLIFFFLVLLKLFKPKNNTKNFPPSPPKLPIIGNLHQLG 64

Query: 66  SLPHRSLAALSEKYGPLMLLNLGHTPTLVVSSAKMARQVMKSHDIIFSNRTQNTAANLLL 125
           SLPH+SLAALS+KYGPLMLL LGH  TLVVSSAKMAR++MK+HD+ FSNR Q   A  LL
Sbjct: 65  SLPHQSLAALSDKYGPLMLLKLGHAQTLVVSSAKMARELMKTHDLKFSNRPQTMPAKTLL 124

Query: 126 YGCHDVAYASYGEYWRQARKICVLELLSAKRVKFFQHVRDEEVAVLINRIREACCVDGYD 185
           YG  DV +A YGEYWRQARKIC LEL SAKRV+ FQ+VRDEEV+VL+NRIR+ C     +
Sbjct: 125 YGFKDVGFAPYGEYWRQARKICALELFSAKRVESFQYVRDEEVSVLVNRIRKGCVDGDGE 184

Query: 186 SSVNLNQLLMSTSNSIVSRCVLGEKFEDENGNSRFGDVTRKVMVLLTAFCITDAFPGFRW 245
            SVNL+QL +STSN+IVSRC+LGEKFEDENG SRFGD +R+VMVL+TAFC  D FP   W
Sbjct: 185 GSVNLSQLFLSTSNNIVSRCILGEKFEDENGKSRFGDTSRRVMVLITAFCFADFFPALWW 244

Query: 246 IDVLRGFTGELKSTFKTLDAFFDKVIEEHMEKMSSDDQPDH-EKDFVDVMHQLQQDDTLD 305
           IDV+RGF GELK  F TLDAF  KV+EEH  KM +  + D  +KDFV +M QL Q D +D
Sbjct: 245 IDVVRGFNGELKKWFTTLDAFLSKVVEEHKAKMRAGGESDESKKDFVGIMLQLMQSDMVD 304

Query: 306 YRFTRDNLKAILL 316
           Y F+ +NLK I+L
Sbjct: 305 YHFSLENLKGIVL 315

[Close]

BLAST of Sgr017609 vs. ExPASy TrEMBL
Match: A0A6J1KG10 (cytochrome P450 71A1-like OS=Cucurbita maxima OX=3661 GN=LOC111495440 PE=3 SV=1)

HSP 1 Score: 403.3 bits (1035), Expect = 9.9e-109
Identity = 214/318 (67.30%), Postives = 251/318 (78.93%), Query Frame = 0


Query: 1   MDPTS-SNLHQFWQQLQAHFNPISFSLCLLFFFLLLLELFGGKRTNS-PPSPPQLPIIGN 60
           MDP S  N+HQ   Q+     P+ F    LF FLL L+LF  K T + PPSPPQLPIIGN
Sbjct: 1   MDPISYKNVHQLLHQITQAPLPL-FLFLFLFLFLLSLKLFSTKSTKALPPSPPQLPIIGN 60

Query: 61  LHQIGSLPHRSLAALSEKYGPLMLLNLGHTPTLVVSSAKMARQVMKSHDIIFSNRTQNTA 120
           LHQ+GSLPHRS+A+LSEKYGPLMLL LG TPTLVVSSAK+AR+VM+SHD IFSNR QN A
Sbjct: 61  LHQLGSLPHRSVASLSEKYGPLMLLKLGQTPTLVVSSAKLAREVMRSHDTIFSNRYQNKA 120

Query: 121 ANLLLYGCHDVAYASYGEYWRQARKICVLELLSAKRVKFFQHVRDEEVAVLINRIREACC 180
           A  L+YGCHDVA+ASYGE WRQARK+CVLELLSAKRV+ FQ+VRDEEV  L+ +IR+ C 
Sbjct: 121 AQSLIYGCHDVAFASYGEQWRQARKLCVLELLSAKRVECFQYVRDEEVGELVKKIRKGC- 180

Query: 181 VDGY-DSSVNLNQLLMSTSNSIVSRCVLGEKFEDENGNSRFGDVTRKVMVLLTAFCITDA 240
             G+ DS VNLNQ+LMSTSN+IVSRCVLGEK E+  G S FGDVTRKVMVLL+AFC+ DA
Sbjct: 181 --GFGDSGVNLNQVLMSTSNNIVSRCVLGEKLENGKGMSGFGDVTRKVMVLLSAFCVGDA 240

Query: 241 FPGFRWIDVLRGFTGELKSTFKTLDAFFDKVIEEHMEKMSSDDQPDHEKDFVDVMHQLQQ 300
            P   W+DV+RGF  ELK+TF+ LD  FD+VI+EH EKM + D+   E DFVD++ QLQQ
Sbjct: 241 LPWLGWVDVVRGFHRELKNTFEALDGLFDRVIDEHREKMKNSDR-SGEMDFVDIILQLQQ 300

Query: 301 DDTLDYRFTRDNLKAILL 316
            DTLDYRFT ++ KAILL
Sbjct: 301 RDTLDYRFTMNDYKAILL 313

[Close]

BLAST of Sgr017609 vs. ExPASy TrEMBL
Match: A0A6J1C631 (cytochrome P450 71A1-like OS=Momordica charantia OX=3673 GN=LOC111008616 PE=3 SV=1)

HSP 1 Score: 399.1 bits (1024), Expect = 1.9e-107
Identity = 208/320 (65.00%), Postives = 246/320 (76.88%), Query Frame = 0


Query: 1   MDPTSSNLHQFWQQLQAHFNPISFSLCLLFFFLLLLELFGGKRT--NSPPSPPQLPIIGN 60
           MDP  S   Q WQQ    F+ I FSLC+ FFFL+LL+L   K    N PPSPP+LPIIGN
Sbjct: 1   MDPI-SKFEQIWQQ----FDLIPFSLCIFFFFLVLLKLLKPKNNTKNFPPSPPKLPIIGN 60

Query: 61  LHQIGSLPHRSLAALSEKYGPLMLLNLGHTPTLVVSSAKMARQVMKSHDIIFSNRTQNTA 120
           LHQ+GSLPH+SLAALS+K+GPLM L LG  PTLVVSSA+MAR+VMK+HD+ FS+R Q TA
Sbjct: 61  LHQLGSLPHQSLAALSDKHGPLMFLKLGQAPTLVVSSAEMAREVMKTHDLNFSDRPQTTA 120

Query: 121 ANLLLYGCHDVAYASYGEYWRQARKICVLELLSAKRVKFFQHVRDEEVAVLINRIREACC 180
           A +LLYG  DV +A YGEYWRQARKIC LEL SAKRV+ FQ+VRDEEVAVL+NRIR  C 
Sbjct: 121 AKILLYGFKDVGFAPYGEYWRQARKICALELFSAKRVESFQYVRDEEVAVLVNRIRRGCV 180

Query: 181 VDGYDSSVNLNQLLMSTSNSIVSRCVLGEKFEDENGNSRFGDVTRKVMVLLTAFCITDAF 240
               + SVNL QL +STSN+IVSRC++G+KFEDENG SRFGD++R+VMVLLTAFC  D F
Sbjct: 181 GGDGEGSVNLAQLFLSTSNNIVSRCMMGDKFEDENGKSRFGDISRRVMVLLTAFCFADFF 240

Query: 241 PGFRWIDVLRGFTGELKSTFKTLDAFFDKVIEEHMEKMSSDDQ--PDHEKDFVDVMHQL- 300
           P   WIDV+RGF GELK  F TLDAFF KV+EEH  KM    +   + ++DF+D M QL 
Sbjct: 241 PALWWIDVVRGFNGELKKCFTTLDAFFSKVVEEHKAKMIGGGELCDESKRDFMDSMLQLI 300

Query: 301 QQDDTLDYRFTRDNLKAILL 316
            QDD +DYRF+ DNLKAI+L
Sbjct: 301 LQDDMVDYRFSLDNLKAIVL 315

[Close]

BLAST of Sgr017609 vs. ExPASy TrEMBL
Match: A0A6J1E7U9 (cytochrome P450 71A1-like OS=Cucurbita moschata OX=3662 GN=LOC111431445 PE=3 SV=1)

HSP 1 Score: 393.7 bits (1010), Expect = 7.8e-106
Identity = 202/321 (62.93%), Postives = 248/321 (77.26%), Query Frame = 0


Query: 1   MDPTSSNLHQFWQQLQ----AHFNPISFSLCLLFFF-LLLLELFGGKR--TNSPPSPPQL 60
           MD TS   H   QQ Q     H NPIS S CL FFF LLLL+LF GK+   N PPSPP+L
Sbjct: 1   MDRTSYFQHWKRQQFQYGVAIHINPISISFCLFFFFLLLLLKLFRGKKQSINFPPSPPKL 60

Query: 61  PIIGNLHQIGSLPHRSLAALSEKYGPLMLLNLGHTPTLVVSSAKMARQVMKSHDIIFSNR 120
           PIIGNLHQ+G+LPH+SLA+LS+KYGPLMLL LG TPTLVVSSAKMAR+VM++HD+ FSNR
Sbjct: 61  PIIGNLHQLGTLPHQSLASLSKKYGPLMLLKLGQTPTLVVSSAKMAREVMRTHDLKFSNR 120

Query: 121 TQNTAANLLLYGCHDVAYASYGEYWRQARKICVLELLSAKRVKFFQHVRDEEVAVLINRI 180
            + TA NLL YGC D+ +A YG YWRQARK+CVLEL S KRV+ FQ++RDEEV VLI+RI
Sbjct: 121 PKTTATNLLFYGCQDMGFAPYGAYWRQARKMCVLELFSFKRVESFQYIRDEEVGVLISRI 180

Query: 181 REACCVDGYDSSVNLNQLLMSTSNSIVSRCVLGEKFEDENGNSRFGDVTRKVMVLLTAFC 240
            +AC        VNL++L +  SN+IVSRCV+G+KFEDENGN+RF DV+R+V+ LL+AFC
Sbjct: 181 HKAC---AGGEVVNLSKLFLQISNNIVSRCVMGKKFEDENGNNRFRDVSRRVIELLSAFC 240

Query: 241 ITDAFPGFRWIDVLRGFTGELKSTFKTLDAFFDKVIEEHMEKMSSDDQPDHEKDFVDVMH 300
           + D FP F W+DV+RGF GELK+  +T+D F D VIEEH  ++ +  +PD ++DF+D+M 
Sbjct: 241 VADFFPAFGWVDVVRGFIGELKTISRTMDEFLDMVIEEHKTELRT-GRPDDKRDFLDIML 300

Query: 301 QLQQDDTLDYRFTRDNLKAIL 315
           QL QDD L Y FTRDNLKAIL
Sbjct: 301 QLNQDDMLGYHFTRDNLKAIL 317

[Close]

BLAST of Sgr017609 vs. TAIR 10
Match: AT3G48310.1 (cytochrome P450, family 71, subfamily A, polypeptide 22 )

HSP 1 Score: 233.4 bits (594), Expect = 2.6e-61
Identity = 119/294 (40.48%), Postives = 188/294 (63.95%), Query Frame = 0


Query: 22  ISFSLCLLFFFLLLLELFGGKRTNSPPSPPQLPIIGNLHQIGSLPHRSLAALSEKYGPLM 81
           I  SL +    L  ++   GK++N+P SPP+LP+IGNLHQ+G  PHRSL +LS +YGPLM
Sbjct: 8   ILLSLIIFITILFFIKQKKGKKSNTPASPPRLPLIGNLHQLGRHPHRSLCSLSNRYGPLM 67

Query: 82  LLNLGHTPTLVVSSAKMARQVMKSHDIIFSNRTQNTAANLLLYGCHDVAYASYGEYWRQA 141
           LL  G  P LVVSSA +AR ++K++D +F++R ++     + Y   DVA A YGEYWRQ 
Sbjct: 68  LLRFGLVPVLVVSSADVARDILKTYDRVFASRPRSKIFEKIFYEARDVALAPYGEYWRQM 127

Query: 142 RKICVLELLSAKRVKFFQHVRDEEVAVLINRIREACCVDGYDSSVNLNQLLMSTSNSIVS 201
           + +CVL LL+ K V+ F++VR EE+++++ +I+++  +      VNL++LL S +N ++S
Sbjct: 128 KSVCVLHLLTNKMVRSFRNVRQEEISLMMEKIQKSSSL-----QVNLSELLGSLTNDVIS 187

Query: 202 RCVLGEKFEDENGNSRFGDVTRKVMVLLTAFCITDAFPGFRWIDVLRGFTGELKSTFKTL 261
           R  LG K+ DE   + F ++ +++  LL  FC+    P   WID + G  G+LK T   L
Sbjct: 188 RVALGRKYSDE---TDFKELMKRLTKLLGEFCVGTYVPWLAWIDWISGLDGQLKKTGNDL 247

Query: 262 DAFFDKVIEEHMEKMSSDDQPDHEKDFVDVMHQLQQDDTLDYRFTRDNLKAILL 316
           D F +KV+++H      +D      DFVDV+ ++Q++ ++ +   R ++KAI+L
Sbjct: 248 DEFLEKVVQDH------EDGDAQRTDFVDVLLRIQREKSVGFEIDRLSIKAIIL 287

[Close]

BLAST of Sgr017609 vs. TAIR 10
Match: AT3G48270.1 (cytochrome P450, family 71, subfamily A, polypeptide 26 )

HSP 1 Score: 229.6 bits (584), Expect = 3.8e-60
Identity = 119/295 (40.34%), Postives = 188/295 (63.73%), Query Frame = 0


Query: 24  FSLCLLFFFLLLL---ELFGGKRTNSPPSPPQLPIIGNLHQIGSLPHRSLAALSEKYGPL 83
           F LC + F + ++   +   GK+ N+ PSPP LP+IGNLHQ+G  PHRSL +LS +YGPL
Sbjct: 6   FLLCSIIFVVTIIIFRKQKRGKKRNTLPSPPGLPLIGNLHQLGRHPHRSLCSLSHRYGPL 65

Query: 84  MLLNLGHTPTLVVSSAKMARQVMKSHDIIFSNRTQNTAANLLLYGCHDVAYASYGEYWRQ 143
           MLL+ G  P LVVSSA++AR V+K+HD +F++R ++     LLY  HDVA A YGEYWRQ
Sbjct: 66  MLLHFGRVPVLVVSSAELARDVLKTHDRVFASRPRSKIFEKLLYDKHDVASAPYGEYWRQ 125

Query: 144 ARKICVLELLSAKRVKFFQHVRDEEVAVLINRIREACCVDGYDSSVNLNQLLMSTSNSIV 203
            + +CVL L S K V+ F+ VR+EE+++++ +IR++  +      VNL+++L+S +N ++
Sbjct: 126 MKSVCVLHLFSNKMVRSFREVREEEISLMMEKIRKSISL-----PVNLSKILVSLTNDVI 185

Query: 204 SRCVLGEKFEDENGNSRFGDVTRKVMVLLTAFCITDAFPGFRWIDVLRGFTGELKSTFKT 263
            +  LG K+    G + F ++  ++  LL  F +    P   WID +RG   +L+ T   
Sbjct: 186 CKVALGRKY---GGETDFKELMERLNKLLGTFSVGSYVPWLAWIDWIRGLDCQLEKTAND 245

Query: 264 LDAFFDKVIEEHMEKMSSDDQPDHEKDFVDVMHQLQQDDTLDYRFTRDNLKAILL 316
           +D FF++V+++H+      D      DFVDV+  +Q+D T+ +   R ++KAI++
Sbjct: 246 VDKFFERVVQDHV------DGNRDMTDFVDVLLAIQRDKTVGFEINRVSIKAIVM 286

[Close]

BLAST of Sgr017609 vs. TAIR 10
Match: AT3G48320.1 (cytochrome P450, family 71, subfamily A, polypeptide 21 )

HSP 1 Score: 228.8 bits (582), Expect = 6.4e-60
Identity = 120/302 (39.74%), Postives = 188/302 (62.25%), Query Frame = 0


Query: 25  SLCLLFFFLLLLELFGGKRTNSPPSPPQLPIIGNLHQIGSLPHRSLAALSEKYGPLMLLN 84
           SL +    L   +   GK++N+P SPP+LP+IGNLHQ+G  PHRSL +LS +YGPLMLL+
Sbjct: 11  SLIIFITILFFKKQKRGKKSNTPRSPPRLPLIGNLHQLGHHPHRSLCSLSHRYGPLMLLH 70

Query: 85  LGHTPTLVVSSAKMARQVMKSHDIIFSNRTQNTAANLLLYGCHDVAYASYGEYWRQARKI 144
           LG  P LVVSSA +AR ++K+HD +F++R ++     L Y   DVA+A YGEYWRQ + +
Sbjct: 71  LGRVPVLVVSSADVARDILKTHDRVFASRPRSKLFEKLFYDGRDVAFAPYGEYWRQIKSV 130

Query: 145 CVLELLSAKRVKFFQHVRDEEVAVLINRIREACCVDGYDSSVNLNQLLMSTSNSIVSRCV 204
           CVL LLS K V  F++VR EE+++++ +I+++  +      VN+++LL S +N ++SR  
Sbjct: 131 CVLRLLSNKMVTSFRNVRQEEISLMMEKIQKSSSL-----QVNVSELLGSLTNDVISRIA 190

Query: 205 LGEKFEDENGNSRFGDVTRKVMVLLTAFCITDAFPGFRWIDVLRGFTGELKSTFKTLDAF 264
           LG K+  E  +    ++ +++M+L+  F +    P   WID + G  G+L  T   LD F
Sbjct: 191 LGRKYSGETDSK---ELMKRLMMLMGEFSVGTYVPWLGWIDWISGLDGQLNKTGNDLDEF 250

Query: 265 FDKVIEEHMEKMSSDDQPDHEKDFVDVMHQLQQDDTLDYRFTRDNLKAILLVSLSLSLSL 324
            +KV+++H+      D      DFVDV+ ++Q++ ++ +   R  +KAI+L  L      
Sbjct: 251 LEKVVQDHV------DGDGQRTDFVDVLLRIQREKSIGFEIDRLCIKAIVLDVLVAGTDS 298

Query: 325 SY 327
           SY
Sbjct: 311 SY 298

[Close]

BLAST of Sgr017609 vs. TAIR 10
Match: AT3G48290.2 (cytochrome P450, family 71, subfamily A, polypeptide 24 )

HSP 1 Score: 220.7 bits (561), Expect = 1.7e-57
Identity = 112/289 (38.75%), Postives = 181/289 (62.63%), Query Frame = 0


Query: 28  LLFFFLLLLELFGGKRTNSPPSPPQLPIIGNLHQIGSLPHRSLAALSEKYGPLMLLNLGH 87
           +L   L   +   GK++N+PPSPP+LP+I NLHQ+G  PHRSL +LS +YGPLMLL+ G 
Sbjct: 13  ILITILFFKKQSRGKKSNAPPSPPRLPLIRNLHQLGRHPHRSLCSLSHRYGPLMLLHFGS 72

Query: 88  TPTLVVSSAKMARQVMKSHDIIFSNRTQNTAANLLLYGCHDVAYASYGEYWRQARKICVL 147
            P LVVSSA  A+ V+K+HD +F++R ++   + + Y   DVA A YGEYWRQ + +CVL
Sbjct: 73  VPVLVVSSADAAKDVLKTHDRVFASRPRSKIFDKIFYNGRDVALAPYGEYWRQMKSVCVL 132

Query: 148 ELLSAKRVKFFQHVRDEEVAVLINRIREACCVDGYDSSVNLNQLLMSTSNSIVSRCVLGE 207
            L S K V+ F+ VR EE++++I +IR +  +      +NL+++L++ +N+++ R  LG 
Sbjct: 133 HLFSNKMVRSFRDVRQEEISLMIEKIRISSSL-----RINLSEILVNLTNNVICRVALGR 192

Query: 208 KFEDENGNSRFGDVTRKVMVLLTAFCITDAFPGFRWIDVLRGFTGELKSTFKTLDAFFDK 267
           K+    G + F D+ +++  LL  F +        WID +RG  G+L      LD F ++
Sbjct: 193 KY---GGKTDFKDLMKRLTRLLGEFSVGSYVSWLAWIDWIRGLDGQLIKISNDLDEFLER 252

Query: 268 VIEEHMEKMSSDDQPDHEKDFVDVMHQLQQDDTLDYRFTRDNLKAILLV 317
           V+++H+      D   H+ DFVD +  ++++ ++ +   R ++KAI+LV
Sbjct: 253 VVQDHV------DGDGHKNDFVDFLLTIEREKSVGFEIDRLSIKAIILV 287

[Close]

BLAST of Sgr017609 vs. TAIR 10
Match: AT3G48290.1 (cytochrome P450, family 71, subfamily A, polypeptide 24 )

HSP 1 Score: 219.2 bits (557), Expect = 5.1e-57
Identity = 111/288 (38.54%), Postives = 180/288 (62.50%), Query Frame = 0


Query: 28  LLFFFLLLLELFGGKRTNSPPSPPQLPIIGNLHQIGSLPHRSLAALSEKYGPLMLLNLGH 87
           +L   L   +   GK++N+PPSPP+LP+I NLHQ+G  PHRSL +LS +YGPLMLL+ G 
Sbjct: 15  ILITILFFKKQSRGKKSNAPPSPPRLPLIRNLHQLGRHPHRSLCSLSHRYGPLMLLHFGS 74

Query: 88  TPTLVVSSAKMARQVMKSHDIIFSNRTQNTAANLLLYGCHDVAYASYGEYWRQARKICVL 147
            P LVVSSA  A+ V+K+HD +F++R ++   + + Y   DVA A YGEYWRQ + +CVL
Sbjct: 75  VPVLVVSSADAAKDVLKTHDRVFASRPRSKIFDKIFYNGRDVALAPYGEYWRQMKSVCVL 134

Query: 148 ELLSAKRVKFFQHVRDEEVAVLINRIREACCVDGYDSSVNLNQLLMSTSNSIVSRCVLGE 207
            L S K V+ F+ VR EE++++I +IR +  +      +NL+++L++ +N+++ R  LG 
Sbjct: 135 HLFSNKMVRSFRDVRQEEISLMIEKIRISSSL-----RINLSEILVNLTNNVICRVALGR 194

Query: 208 KFEDENGNSRFGDVTRKVMVLLTAFCITDAFPGFRWIDVLRGFTGELKSTFKTLDAFFDK 267
           K+    G + F D+ +++  LL  F +        WID +RG  G+L      LD F ++
Sbjct: 195 KY---GGKTDFKDLMKRLTRLLGEFSVGSYVSWLAWIDWIRGLDGQLIKISNDLDEFLER 254

Query: 268 VIEEHMEKMSSDDQPDHEKDFVDVMHQLQQDDTLDYRFTRDNLKAILL 316
           V+++H+      D   H+ DFVD +  ++++ ++ +   R ++KAI+L
Sbjct: 255 VVQDHV------DGDGHKNDFVDFLLTIEREKSVGFEIDRLSIKAIIL 288

The following BLAST results are available for this feature:

BLAST of Sgr017609 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022137028.1	2.0e-119	69.63	cytochrome P450 71A1-like [Momordica charantia]	[more]
XP_022137055.1	1.3e-110	66.77	cytochrome P450 71A1-like [Momordica charantia]	[more]
XP_023001252.1	2.0e-108	67.30	cytochrome P450 71A1-like [Cucurbita maxima]	[more]
XP_023520090.1	1.0e-107	66.98	cytochrome P450 71A1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022137054.1	3.8e-107	65.00	cytochrome P450 71A1-like [Momordica charantia]	[more]

BLAST of Sgr017609 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A068Q609	4.3e-77	47.95	Phenylacetaldehyde oxime monooxygenase CYP71AN24 OS=Prunus mume OX=102107 GN=CYP...	[more]
P24465	1.4e-72	46.60	Cytochrome P450 71A1 OS=Persea americana OX=3435 GN=CYP71A1 PE=1 SV=2	[more]
A0A068Q721	3.9e-62	41.31	Cytochrome P450 71AP13 OS=Prunus mume OX=102107 GN=CYP71AP13 PE=2 SV=1	[more]
Q9STL1	3.7e-60	40.48	Cytochrome P450 71A22 OS=Arabidopsis thaliana OX=3702 GN=CYP71A22 PE=2 SV=1	[more]
P37117	4.8e-60	41.50	Cytochrome P450 71A4 OS=Solanum melongena OX=4111 GN=CYP71A4 PE=2 SV=1	[more]

BLAST of Sgr017609 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C953	9.5e-120	69.63	cytochrome P450 71A1-like OS=Momordica charantia OX=3673 GN=LOC111008589 PE=3 SV...	[more]
A0A6J1C5L5	6.2e-111	66.77	cytochrome P450 71A1-like OS=Momordica charantia OX=3673 GN=LOC111008617 PE=3 SV...	[more]
A0A6J1KG10	9.9e-109	67.30	cytochrome P450 71A1-like OS=Cucurbita maxima OX=3661 GN=LOC111495440 PE=3 SV=1	[more]
A0A6J1C631	1.9e-107	65.00	cytochrome P450 71A1-like OS=Momordica charantia OX=3673 GN=LOC111008616 PE=3 SV...	[more]
A0A6J1E7U9	7.8e-106	62.93	cytochrome P450 71A1-like OS=Cucurbita moschata OX=3662 GN=LOC111431445 PE=3 SV=...	[more]

BLAST of Sgr017609 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G48310.1	2.6e-61	40.48	cytochrome P450, family 71, subfamily A, polypeptide 22	[more]
AT3G48270.1	3.8e-60	40.34	cytochrome P450, family 71, subfamily A, polypeptide 26	[more]
AT3G48320.1	6.4e-60	39.74	cytochrome P450, family 71, subfamily A, polypeptide 21	[more]
AT3G48290.2	1.7e-57	38.75	cytochrome P450, family 71, subfamily A, polypeptide 24	[more]
AT3G48290.1	5.1e-57	38.54	cytochrome P450, family 71, subfamily A, polypeptide 24	[more]

InterPro

Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 74..93 score: 35.56 coord: 195..213 score: 17.01 coord: 98..119 score: 18.93
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 47..315 e-value: 2.5E-31 score: 109.0
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 40..320 e-value: 1.5E-52 score: 180.7
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 45..317
None	No IPR available	PANTHER	PTHR24281:SF210	CYTOCHROME P450 71A1-LIKE	coord: 26..315
None	No IPR available	PANTHER	PTHR24281	STEROID 21-HYDROXYLASE-RELATED	coord: 26..315

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sgr017609.1	Sgr017609.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen