Sgr017320 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr017320
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Descriptionpolygalacturonase inhibitor-like
Locationtig00153037: 1129298 .. 1130305 (-)
RNA-Seq ExpressionSgr017320
SyntenySgr017320
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTTCCTCGTAACCGCATAACTCCATTTCCGGCCACCTTCCTCTTCCTCTTCCTCTTCCTCTTCCTCTTCACCATTTCTGTTGCAGAACGCTGCAACCCAGATGACAAAAAAGTCCTCCTCAAAATCAAGAAGGCCTTCAATGACCCCTACATCCTCGCCTCCTGGAAACCAGAGGAAGACTGCTGCACCTGGTACTGTGTCGACTGCCACCCCTCCTCCCACCGCATCATCTCCCTCACCATCTTCTCCGGCGAGCTCTCCGGCCAAATACCCCCAGAGGTCGGCGATCTCCCTTTCCTAGAAACCCTCATGTTTCACAAGCTCTCCAACCTCACCGGTCCCATCCAACCCGCCATAGCCAAGCTTCGCCACCTCAAGTCTCTCGACCTCAGCTGGACCAATCTCTCCGGACCCATCCCTGGTTTTCTCGGCAGCCTCACTGGCCTCACTTATCTAGACCTCTCCTTCAACCACCTCACCGGACCCATCCCGAGCTCGCTCGCCAACCTCCCAAACCTCGGCGCCCTCCATTTGGACCGAAACAAACTCACCGGACCCATCCCGGATTCGTTCGGACAGTTCAAGGGACAAGTTCCGTACCTTTATCTCTCCCACAACCAACTTTCCGGGAAAATCCCAGCTTCCTTGGCCAGAGTGAACTTCAATGTGATTGACTTGTCGCGGAACAAGCTCGAAGGCGACGCGTCGGTGATATTCGGGGGAAAGAAGACGACGGAGATCGTGGATCTGTCGAGGAACTTGTTGGAGTTCAACTTGTCGAAGGTTGTGTTTCCGAAGAGCCTGACTTGGTTGGATCTTAACCATAACAGGATTTTCGGGGGGATTCCGGCGACGGTGGCGAAGCTAGAACTGCAGTTGCTGAATGTGAGTTACAATAGGCTGTGTGGTCGGATTCCGGTCGGTGGGAGACTGCAGAGCTTCGATCTCTACTCGTATTTTCACAACAAGTGCTTGTGCGGCAAACCACTTGGCTCCTGCAAGTGA

mRNA sequence

ATGCTTCCTCGTAACCGCATAACTCCATTTCCGGCCACCTTCCTCTTCCTCTTCCTCTTCCTCTTCCTCTTCACCATTTCTGTTGCAGAACGCTGCAACCCAGATGACAAAAAAGTCCTCCTCAAAATCAAGAAGGCCTTCAATGACCCCTACATCCTCGCCTCCTGGAAACCAGAGGAAGACTGCTGCACCTGGTACTGTGTCGACTGCCACCCCTCCTCCCACCGCATCATCTCCCTCACCATCTTCTCCGGCGAGCTCTCCGGCCAAATACCCCCAGAGGTCGGCGATCTCCCTTTCCTAGAAACCCTCATGTTTCACAAGCTCTCCAACCTCACCGGTCCCATCCAACCCGCCATAGCCAAGCTTCGCCACCTCAAGTCTCTCGACCTCAGCTGGACCAATCTCTCCGGACCCATCCCTGGTTTTCTCGGCAGCCTCACTGGCCTCACTTATCTAGACCTCTCCTTCAACCACCTCACCGGACCCATCCCGAGCTCGCTCGCCAACCTCCCAAACCTCGGCGCCCTCCATTTGGACCGAAACAAACTCACCGGACCCATCCCGGATTCGTTCGGACAGTTCAAGGGACAAGTTCCGTACCTTTATCTCTCCCACAACCAACTTTCCGGGAAAATCCCAGCTTCCTTGGCCAGAGTGAACTTCAATGTGATTGACTTGTCGCGGAACAAGCTCGAAGGCGACGCGTCGGTGATATTCGGGGGAAAGAAGACGACGGAGATCGTGGATCTGTCGAGGAACTTGTTGGAGTTCAACTTGTCGAAGGTTGTGTTTCCGAAGAGCCTGACTTGGTTGGATCTTAACCATAACAGGATTTTCGGGGGGATTCCGGCGACGGTGGCGAAGCTAGAACTGCAGTTGCTGAATGTGAGTTACAATAGGCTGTGTGGTCGGATTCCGGTCGGTGGGAGACTGCAGAGCTTCGATCTCTACTCGTATTTTCACAACAAGTGCTTGTGCGGCAAACCACTTGGCTCCTGCAAGTGA

Coding sequence (CDS)

ATGCTTCCTCGTAACCGCATAACTCCATTTCCGGCCACCTTCCTCTTCCTCTTCCTCTTCCTCTTCCTCTTCACCATTTCTGTTGCAGAACGCTGCAACCCAGATGACAAAAAAGTCCTCCTCAAAATCAAGAAGGCCTTCAATGACCCCTACATCCTCGCCTCCTGGAAACCAGAGGAAGACTGCTGCACCTGGTACTGTGTCGACTGCCACCCCTCCTCCCACCGCATCATCTCCCTCACCATCTTCTCCGGCGAGCTCTCCGGCCAAATACCCCCAGAGGTCGGCGATCTCCCTTTCCTAGAAACCCTCATGTTTCACAAGCTCTCCAACCTCACCGGTCCCATCCAACCCGCCATAGCCAAGCTTCGCCACCTCAAGTCTCTCGACCTCAGCTGGACCAATCTCTCCGGACCCATCCCTGGTTTTCTCGGCAGCCTCACTGGCCTCACTTATCTAGACCTCTCCTTCAACCACCTCACCGGACCCATCCCGAGCTCGCTCGCCAACCTCCCAAACCTCGGCGCCCTCCATTTGGACCGAAACAAACTCACCGGACCCATCCCGGATTCGTTCGGACAGTTCAAGGGACAAGTTCCGTACCTTTATCTCTCCCACAACCAACTTTCCGGGAAAATCCCAGCTTCCTTGGCCAGAGTGAACTTCAATGTGATTGACTTGTCGCGGAACAAGCTCGAAGGCGACGCGTCGGTGATATTCGGGGGAAAGAAGACGACGGAGATCGTGGATCTGTCGAGGAACTTGTTGGAGTTCAACTTGTCGAAGGTTGTGTTTCCGAAGAGCCTGACTTGGTTGGATCTTAACCATAACAGGATTTTCGGGGGGATTCCGGCGACGGTGGCGAAGCTAGAACTGCAGTTGCTGAATGTGAGTTACAATAGGCTGTGTGGTCGGATTCCGGTCGGTGGGAGACTGCAGAGCTTCGATCTCTACTCGTATTTTCACAACAAGTGCTTGTGCGGCAAACCACTTGGCTCCTGCAAGTGA

Protein sequence

MLPRNRITPFPATFLFLFLFLFLFTISVAERCNPDDKKVLLKIKKAFNDPYILASWKPEEDCCTWYCVDCHPSSHRIISLTIFSGELSGQIPPEVGDLPFLETLMFHKLSNLTGPIQPAIAKLRHLKSLDLSWTNLSGPIPGFLGSLTGLTYLDLSFNHLTGPIPSSLANLPNLGALHLDRNKLTGPIPDSFGQFKGQVPYLYLSHNQLSGKIPASLARVNFNVIDLSRNKLEGDASVIFGGKKTTEIVDLSRNLLEFNLSKVVFPKSLTWLDLNHNRIFGGIPATVAKLELQLLNVSYNRLCGRIPVGGRLQSFDLYSYFHNKCLCGKPLGSCK
Homology
BLAST of Sgr017320 vs. NCBI nr
Match: XP_022151650.1 (polygalacturonase inhibitor-like [Momordica charantia] >ARM53420.1 pgip1 [Momordica charantia])

HSP 1 Score: 558.1 bits (1437), Expect = 5.0e-155
Identity = 264/321 (82.24%), Postives = 285/321 (88.79%), Query Frame = 0

Query: 16  FLFLFLFLFTISVAERCNPDDKKVLLKIKKAFNDPYILASWKPEEDCCTWYCVDCHPSSH 75
           F FL     +I  AE CNP+DKKVLLKIKK+FN+PYIL SWKPEEDCC+WYCV CHP+SH
Sbjct: 8   FFFLLFISSSICFAELCNPNDKKVLLKIKKSFNNPYILTSWKPEEDCCSWYCVQCHPTSH 67

Query: 76  RIISLTIFSGE-LSGQIPPEVGDLPFLETLMFHKLSNLTGPIQPAIAKLRHLKSLDLSWT 135
           RIISLTIF+ + LSGQIPP VGDLPFLETLM HKL NLTGPI  A+A L +LKSLDLSW 
Sbjct: 68  RIISLTIFADDKLSGQIPPVVGDLPFLETLMLHKLPNLTGPIPAAVANLHNLKSLDLSWN 127

Query: 136 NLSGPIPGFLGSLTGLTYLDLSFNHLTGPIPSSLANLPNLGALHLDRNKLTGPIPDSFGQ 195
            LSGP+P FLGSL+ LTYLDLSFN+LTG IPSSLANL  LGALHLDRNKLTGPIP SFG 
Sbjct: 128 GLSGPVPEFLGSLSSLTYLDLSFNNLTGSIPSSLANLQRLGALHLDRNKLTGPIPHSFGY 187

Query: 196 FKGQVPYLYLSHNQLSGKIPASLARVNFNVIDLSRNKLEGDASVIFGGKKTTEIVDLSRN 255
           FKG++PYLYLSHNQLSGKIPASL RVNFNVIDLSRNKLEGDASVIFGG KTTEIVDLSRN
Sbjct: 188 FKGKIPYLYLSHNQLSGKIPASLGRVNFNVIDLSRNKLEGDASVIFGGNKTTEIVDLSRN 247

Query: 256 LLEFNLSKVVFPKSLTWLDLNHNRIFGGIPATVAKLELQLLNVSYNRLCGRIPVGGRLQS 315
           LLEF+LSKVVFP+S+TWLDLNHN+IFGGIP  V +LELQLLNVSYNRLCGRIPVGGRLQ 
Sbjct: 248 LLEFDLSKVVFPRSVTWLDLNHNKIFGGIPEKVVELELQLLNVSYNRLCGRIPVGGRLQR 307

Query: 316 FDLYSYFHNKCLCGKPLGSCK 336
           FD+Y YFHNKCLCGKPLGSCK
Sbjct: 308 FDVYEYFHNKCLCGKPLGSCK 328

BLAST of Sgr017320 vs. NCBI nr
Match: XP_038880971.1 (polygalacturonase inhibitor-like [Benincasa hispida])

HSP 1 Score: 539.7 bits (1389), Expect = 1.8e-149
Identity = 256/322 (79.50%), Postives = 276/322 (85.71%), Query Frame = 0

Query: 15  LFLFLFLFLFTISVAERCNPDDKKVLLKIKKAFNDPYILASWKPEEDCCTWYCVDCHPSS 74
           L L LFLF FT S AE CNPDDKKVLL IKKAFN+PYIL SWKPEEDCCTWYCV+C   S
Sbjct: 16  LLLLLFLFFFTCSFAELCNPDDKKVLLNIKKAFNNPYILTSWKPEEDCCTWYCVECDKKS 75

Query: 75  HRIISLTIFSGE-LSGQIPPEVGDLPFLETLMFHKLSNLTGPIQPAIAKLRHLKSLDLSW 134
           HRII+LTIF+ + L GQIPP VGDLPFLE LMFHKL NL GPI P IAKL  LK LDLSW
Sbjct: 76  HRIIALTIFADDKLKGQIPPSVGDLPFLENLMFHKLPNLVGPIPPTIAKLHKLKYLDLSW 135

Query: 135 TNLSGPIPGFLGSLTGLTYLDLSFNHLTGPIPSSLANLPNLGALHLDRNKLTGPIPDSFG 194
             LSG IP FL SL+ L YLDLSFN LTG IPSSLANL +LG LHLDRNKLTGPIPDSFG
Sbjct: 136 NGLSGSIPSFLDSLSDLDYLDLSFNRLTGSIPSSLANLRHLGTLHLDRNKLTGPIPDSFG 195

Query: 195 QFKGQVPYLYLSHNQLSGKIPASLARVNFNVIDLSRNKLEGDASVIFGGKKTTEIVDLSR 254
            FKG++PYLYLSHNQLSGKIPAS+  V+FN IDLSRNKLEGDASV+FG KKTTEI++LSR
Sbjct: 196 NFKGKIPYLYLSHNQLSGKIPASMGGVDFNYIDLSRNKLEGDASVLFGAKKTTEIINLSR 255

Query: 255 NLLEFNLSKVVFPKSLTWLDLNHNRIFGGIPATVAKLELQLLNVSYNRLCGRIPVGGRLQ 314
           NLLEF++SKVVFP +LTWLDLNHN+IFG IP  V KLELQ+LNVSYN LCGRIPVGG+LQ
Sbjct: 256 NLLEFDMSKVVFPPTLTWLDLNHNKIFGEIPMGVVKLELQMLNVSYNALCGRIPVGGKLQ 315

Query: 315 SFDLYSYFHNKCLCGKPLGSCK 336
           SFD YSYFHNKCLCGKPLGSCK
Sbjct: 316 SFDAYSYFHNKCLCGKPLGSCK 337

BLAST of Sgr017320 vs. NCBI nr
Match: XP_022928674.1 (polygalacturonase inhibitor-like [Cucurbita moschata] >XP_023543570.1 polygalacturonase inhibitor-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 538.5 bits (1386), Expect = 4.1e-149
Identity = 258/326 (79.14%), Postives = 283/326 (86.81%), Query Frame = 0

Query: 11  PATFLFLFLFLFLFTISVAERCNPDDKKVLLKIKKAFNDPYILASWKPEEDCCTWYCVDC 70
           P+TFLF+F FLF FTIS AE C+P+DKK LL IKKAFN+PYIL SWKPEEDCCTWYCV+C
Sbjct: 5   PSTFLFIF-FLF-FTISFAELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCTWYCVEC 64

Query: 71  HPSSHRIISLTIFSG-ELSGQIPPEVGDLPFLETLMFHKLSNLTGPIQPAIAKLRHLKSL 130
              SHRI+SLTIF+   LSGQIPP VGDLP+LE LMFHKL NLTGPIQPAIAKL +LK L
Sbjct: 65  DRKSHRIVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLHNLKYL 124

Query: 131 DLSWTNLSGPIPGFLGSLTGLTYLDLSFNHLTGPIPSSLANLPNLGALHLDRNKLTGPIP 190
           DLSW +L+G IP FL SLT L  LDLSFN LTG IPSSLANL  LG LHLDRNKLTGPIP
Sbjct: 125 DLSWNSLTGSIPNFLSSLTNLDLLDLSFNRLTGSIPSSLANLQRLGTLHLDRNKLTGPIP 184

Query: 191 DSFGQFKGQVPYLYLSHNQLSGKIPASLARVNFNVIDLSRNKLEGDASVIFGGKKTTEIV 250
           DSFG FKG++PYLYLSHNQLSGKIP SL RV+FNVIDLSRN+LEGD SVIFGGKKTTEIV
Sbjct: 185 DSFGNFKGKIPYLYLSHNQLSGKIPTSLERVDFNVIDLSRNRLEGDGSVIFGGKKTTEIV 244

Query: 251 DLSRNLLEFNLSKVVFPKSLTWLDLNHNRIFGGIPATVAKLELQLLNVSYNRLCGRIPVG 310
           DLSRNLLEF++SKVV P+SLTWLDLNHN+IFG IP     L+LQLLNVSYN+LCGRIPVG
Sbjct: 245 DLSRNLLEFDMSKVVLPRSLTWLDLNHNKIFGEIPVMAVNLQLQLLNVSYNKLCGRIPVG 304

Query: 311 GRLQSFDLYSYFHNKCLCGKPLGSCK 336
           G+LQSFD+YSY +NKCLCGKPLG CK
Sbjct: 305 GKLQSFDVYSYSNNKCLCGKPLGKCK 328

BLAST of Sgr017320 vs. NCBI nr
Match: KAG6603233.1 (Polygalacturonase inhibitor, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 537.3 bits (1383), Expect = 9.2e-149
Identity = 258/326 (79.14%), Postives = 282/326 (86.50%), Query Frame = 0

Query: 11  PATFLFLFLFLFLFTISVAERCNPDDKKVLLKIKKAFNDPYILASWKPEEDCCTWYCVDC 70
           P+TFLF+F FLF FTIS AE C+P+DKK LL IKKAFN+PYIL SWKPEEDCCTWYCV C
Sbjct: 5   PSTFLFIF-FLF-FTISFAELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCTWYCVKC 64

Query: 71  HPSSHRIISLTIFSG-ELSGQIPPEVGDLPFLETLMFHKLSNLTGPIQPAIAKLRHLKSL 130
              SHRI+SLTIF+   LSGQIPP VGDLP+LE LMFHKL NLTGPIQPAIAKL +LK L
Sbjct: 65  DRKSHRIVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLHNLKYL 124

Query: 131 DLSWTNLSGPIPGFLGSLTGLTYLDLSFNHLTGPIPSSLANLPNLGALHLDRNKLTGPIP 190
           DLSW +L+G IP FL SLT L  LDLSFN LTG IPSSLANL  LG LHLDRNKLTGPIP
Sbjct: 125 DLSWNSLTGSIPNFLSSLTNLDILDLSFNRLTGSIPSSLANLQRLGTLHLDRNKLTGPIP 184

Query: 191 DSFGQFKGQVPYLYLSHNQLSGKIPASLARVNFNVIDLSRNKLEGDASVIFGGKKTTEIV 250
           DSFG FKG++PYLYLSHNQLSGKIP SL RV+FNVIDLSRN+LEGD SVIFGGKKTTEIV
Sbjct: 185 DSFGNFKGKIPYLYLSHNQLSGKIPTSLERVDFNVIDLSRNRLEGDGSVIFGGKKTTEIV 244

Query: 251 DLSRNLLEFNLSKVVFPKSLTWLDLNHNRIFGGIPATVAKLELQLLNVSYNRLCGRIPVG 310
           DLSRNLLEF++SKVV P+SLTWLDLNHN+IFG IP     L+LQLLNVSYN+LCGRIPVG
Sbjct: 245 DLSRNLLEFDMSKVVLPRSLTWLDLNHNKIFGEIPVMAVNLQLQLLNVSYNKLCGRIPVG 304

Query: 311 GRLQSFDLYSYFHNKCLCGKPLGSCK 336
           G+LQSFD+YSY +NKCLCGKPLG CK
Sbjct: 305 GKLQSFDVYSYSNNKCLCGKPLGKCK 328

BLAST of Sgr017320 vs. NCBI nr
Match: XP_022967838.1 (polygalacturonase inhibitor-like [Cucurbita maxima])

HSP 1 Score: 534.6 bits (1376), Expect = 5.9e-148
Identity = 256/326 (78.53%), Postives = 281/326 (86.20%), Query Frame = 0

Query: 11  PATFLFLFLFLFLFTISVAERCNPDDKKVLLKIKKAFNDPYILASWKPEEDCCTWYCVDC 70
           P+TFL +F FLF FTIS AE C+P+DKK LL IKKAFN+PYIL SWKPEEDCCTWYCV+C
Sbjct: 5   PSTFLLIF-FLF-FTISFAELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCTWYCVEC 64

Query: 71  HPSSHRIISLTIFSG-ELSGQIPPEVGDLPFLETLMFHKLSNLTGPIQPAIAKLRHLKSL 130
             +SHRI+SLTIF+   LSGQIPP VGDLP+LE LMFHKL NLTGPIQPAIAKL +LK L
Sbjct: 65  DRNSHRIVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLHNLKYL 124

Query: 131 DLSWTNLSGPIPGFLGSLTGLTYLDLSFNHLTGPIPSSLANLPNLGALHLDRNKLTGPIP 190
           DLSW  L+G IP FL SLT L  LDLSFN LTG IPSSLANL  LG LHLDRNKLTGPIP
Sbjct: 125 DLSWNTLTGSIPNFLSSLTNLELLDLSFNRLTGSIPSSLANLQRLGTLHLDRNKLTGPIP 184

Query: 191 DSFGQFKGQVPYLYLSHNQLSGKIPASLARVNFNVIDLSRNKLEGDASVIFGGKKTTEIV 250
           DSFG FKG++PYLYLSHNQLSGKIP S  RV+FNVIDLSRN+LEGD SVIFGGKKTTEIV
Sbjct: 185 DSFGNFKGKIPYLYLSHNQLSGKIPTSFERVDFNVIDLSRNRLEGDGSVIFGGKKTTEIV 244

Query: 251 DLSRNLLEFNLSKVVFPKSLTWLDLNHNRIFGGIPATVAKLELQLLNVSYNRLCGRIPVG 310
           DLSRNLLEF++SKVV P+SLTWLDLNHN+IFG IP     L+LQLLNVSYN+LCGRIPVG
Sbjct: 245 DLSRNLLEFDMSKVVLPRSLTWLDLNHNKIFGKIPVMAVNLQLQLLNVSYNKLCGRIPVG 304

Query: 311 GRLQSFDLYSYFHNKCLCGKPLGSCK 336
           G+LQSFD+YSY +NKCLCGKPLG CK
Sbjct: 305 GKLQSFDVYSYSNNKCLCGKPLGKCK 328

BLAST of Sgr017320 vs. ExPASy Swiss-Prot
Match: Q05091 (Polygalacturonase inhibitor OS=Pyrus communis OX=23211 GN=PGIP PE=1 SV=1)

HSP 1 Score: 482.6 bits (1241), Expect = 3.5e-135
Identity = 234/325 (72.00%), Postives = 266/325 (81.85%), Query Frame = 0

Query: 12  ATFLFL-FLFLFLFTISVAERCNPDDKKVLLKIKKAFNDPYILASWKPEEDCCTWYCVDC 71
           +TFL L  LF  +   ++++ CNPDDKKVLL+IKKAF DPY+LASWK + DCC WYCV C
Sbjct: 6   STFLSLTLLFSSVLNPALSDLCNPDDKKVLLQIKKAFGDPYVLASWKSDTDCCDWYCVTC 65

Query: 72  HPSSHRIISLTIFSGELSGQIPPEVGDLPFLETLMFHKLSNLTGPIQPAIAKLRHLKSLD 131
             +++RI SLTIF+G++SGQIP  VGDLP+LETL FHK  NLTGPIQPAIAKL+ LKSL 
Sbjct: 66  DSTTNRINSLTIFAGQVSGQIPALVGDLPYLETLEFHKQPNLTGPIQPAIAKLKGLKSLR 125

Query: 132 LSWTNLSGPIPGFLGSLTGLTYLDLSFNHLTGPIPSSLANLPNLGALHLDRNKLTGPIPD 191
           LSWTNLSG +P FL  L  LT+LDLSFN+LTG IPSSL+ LPNLGAL LDRNKLTG IP 
Sbjct: 126 LSWTNLSGSVPDFLSQLKNLTFLDLSFNNLTGAIPSSLSELPNLGALRLDRNKLTGHIPI 185

Query: 192 SFGQFKGQVPYLYLSHNQLSGKIPASLARVNFNVIDLSRNKLEGDASVIFGGKKTTEIVD 251
           SFGQF G VP LYLSHNQLSG IP S A+++F  IDLSRNKLEGDASVIFG  KTT+IVD
Sbjct: 186 SFGQFIGNVPDLYLSHNQLSGNIPTSFAQMDFTSIDLSRNKLEGDASVIFGLNKTTQIVD 245

Query: 252 LSRNLLEFNLSKVVFPKSLTWLDLNHNRIFGGIPATVAKLELQLLNVSYNRLCGRIPVGG 311
           LSRNLLEFNLSKV FP SLT LD+NHN+I+G IP    +L  Q LNVSYNRLCG+IPVGG
Sbjct: 246 LSRNLLEFNLSKVEFPTSLTSLDINHNKIYGSIPVEFTQLNFQFLNVSYNRLCGQIPVGG 305

Query: 312 RLQSFDLYSYFHNKCLCGKPLGSCK 336
           +LQSFD YSYFHN+CLCG PL SCK
Sbjct: 306 KLQSFDEYSYFHNRCLCGAPLPSCK 330

BLAST of Sgr017320 vs. ExPASy Swiss-Prot
Match: A7PW81 (Polygalacturonase inhibitor OS=Vitis vinifera OX=29760 GN=pgip PE=1 SV=1)

HSP 1 Score: 473.4 bits (1217), Expect = 2.1e-132
Identity = 220/309 (71.20%), Postives = 254/309 (82.20%), Query Frame = 0

Query: 27  SVAERCNPDDKKVLLKIKKAFNDPYILASWKPEEDCCTWYCVDCHPSSHRIISLTIFSGE 86
           S++ERCNP DKKVLL+IKKA ++PYILASW P  DCC WYCV+C  ++HRI SLTIFSG+
Sbjct: 25  SLSERCNPKDKKVLLQIKKALDNPYILASWNPNTDCCGWYCVECDLTTHRINSLTIFSGQ 84

Query: 87  LSGQIPPEVGDLPFLETLMFHKLSNLTGPIQPAIAKLRHLKSLDLSWTNLSGPIPGFLGS 146
           LSGQIP  VGDLPFLETL+F KLSNLTG I PAIAKL+HLK + LSWTNLSGP+P F   
Sbjct: 85  LSGQIPDAVGDLPFLETLIFRKLSNLTGQIPPAIAKLKHLKMVRLSWTNLSGPVPAFFSE 144

Query: 147 LTGLTYLDLSFNHLTGPIPSSLANLPNLGALHLDRNKLTGPIPDSFGQFKGQVPYLYLSH 206
           L  LTYLDLSFN+L+GPIP SL+ LPNLGALHLDRN LTGPIPDSFG+F G  P L+LSH
Sbjct: 145 LKNLTYLDLSFNNLSGPIPGSLSLLPNLGALHLDRNHLTGPIPDSFGKFAGSTPGLHLSH 204

Query: 207 NQLSGKIPASLARVNFNVIDLSRNKLEGDASVIFGGKKTTEIVDLSRNLLEFNLSKVVFP 266
           NQLSGKIP S    + NV+DLSRNKLEGD S+ F   K+T+IVD SRNL +F+LS+V FP
Sbjct: 205 NQLSGKIPYSFRGFDPNVMDLSRNKLEGDLSIFFNANKSTQIVDFSRNLFQFDLSRVEFP 264

Query: 267 KSLTWLDLNHNRIFGGIPATVAKLELQLLNVSYNRLCGRIPVGGRLQSFDLYSYFHNKCL 326
           KSLT LDL+HN+I G +P  +  L+LQ LNVSYNRLCG+IPVGG+LQSFD  SYFHN+CL
Sbjct: 265 KSLTSLDLSHNKIAGSLPEMMTSLDLQFLNVSYNRLCGKIPVGGKLQSFDYDSYFHNRCL 324

Query: 327 CGKPLGSCK 336
           CG PL SCK
Sbjct: 325 CGAPLQSCK 333

BLAST of Sgr017320 vs. ExPASy Swiss-Prot
Match: Q9M5J9 (Polygalacturonase inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=PGIP1 PE=1 SV=1)

HSP 1 Score: 434.5 bits (1116), Expect = 1.1e-120
Identity = 206/326 (63.19%), Postives = 248/326 (76.07%), Query Frame = 0

Query: 12  ATFLFLFLFLFLFTISVAERCNPDDKKVLLKIKKAFNDPYILASWKPEEDCCTWYCVDCH 71
           AT   LFLF FL T    + CN +DK  LLKIKK+ N+PY LASW P+ DCC+WYC++C 
Sbjct: 5   ATLCLLFLFTFLTTCLSKDLCNQNDKNTLLKIKKSLNNPYHLASWDPQTDCCSWYCLECG 64

Query: 72  PS--SHRIISLTIFSGELSGQIPPEVGDLPFLETLMFHKLSNLTGPIQPAIAKLRHLKSL 131
            +  +HR+ +LTIFSG++SGQIP EVGDLP+LETL+F KLSNLTG IQP IAKL++L+ L
Sbjct: 65  DATVNHRVTALTIFSGQISGQIPAEVGDLPYLETLVFRKLSNLTGTIQPTIAKLKNLRML 124

Query: 132 DLSWTNLSGPIPGFLGSLTGLTYLDLSFNHLTGPIPSSLANLPNLGALHLDRNKLTGPIP 191
            LSWTNL+GPIP F+  L  L +L+LSFN L+G IPSSL+ LP + AL L RNKLTG IP
Sbjct: 125 RLSWTNLTGPIPDFISQLKNLEFLELSFNDLSGSIPSSLSTLPKILALELSRNKLTGSIP 184

Query: 192 DSFGQFKGQVPYLYLSHNQLSGKIPASLARVNFNVIDLSRNKLEGDASVIFGGKKTTEIV 251
           +SFG F G VP L LSHNQLSG IP SL  ++FN IDLSRNKL+GDAS++FG  KTT  +
Sbjct: 185 ESFGSFPGTVPDLRLSHNQLSGPIPKSLGNIDFNRIDLSRNKLQGDASMLFGSNKTTWSI 244

Query: 252 DLSRNLLEFNLSKVVFPKSLTWLDLNHNRIFGGIPATVAKLELQLLNVSYNRLCGRIPVG 311
           DLSRN+ +F++SKV  PK+L  LDLNHN I G IP    +  LQ  NVSYN+LCG IP G
Sbjct: 245 DLSRNMFQFDISKVDIPKTLGILDLNHNGITGNIPVQWTEAPLQFFNVSYNKLCGHIPTG 304

Query: 312 GRLQSFDLYSYFHNKCLCGKPLGSCK 336
           G+LQ+FD YSYFHNKCLCG PL  CK
Sbjct: 305 GKLQTFDSYSYFHNKCLCGAPLEICK 330

BLAST of Sgr017320 vs. ExPASy Swiss-Prot
Match: Q9M5J8 (Polygalacturonase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PGIP2 PE=2 SV=2)

HSP 1 Score: 423.3 bits (1087), Expect = 2.5e-117
Identity = 206/324 (63.58%), Postives = 247/324 (76.23%), Query Frame = 0

Query: 15  LFLFLFLFLFTISVA-ERCNPDDKKVLLKIKKAFNDPYILASWKPEEDCCTWYCVDCHPS 74
           LFL L   L T S+A + C+ DDK  LLKIKK+ N+PY LASW P+ DCC+WYC++C  +
Sbjct: 7   LFLLLSTLLLTTSLAKDLCHKDDKTTLLKIKKSLNNPYHLASWDPKTDCCSWYCLECGDA 66

Query: 75  --SHRIISLTIFSGELSGQIPPEVGDLPFLETLMFHKLSNLTGPIQPAIAKLRHLKSLDL 134
             +HR+ SL I  GE+SGQIPPEVGDLP+L +L+F KL+NLTG IQP IAKL++L  L L
Sbjct: 67  TVNHRVTSLIIQDGEISGQIPPEVGDLPYLTSLIFRKLTNLTGHIQPTIAKLKNLTFLRL 126

Query: 135 SWTNLSGPIPGFLGSLTGLTYLDLSFNHLTGPIPSSLANLPNLGALHLDRNKLTGPIPDS 194
           SWTNL+GP+P FL  L  L Y+DLSFN L+G IPSSL++L  L  L L RNKLTGPIP+S
Sbjct: 127 SWTNLTGPVPEFLSQLKNLEYIDLSFNDLSGSIPSSLSSLRKLEYLELSRNKLTGPIPES 186

Query: 195 FGQFKGQVPYLYLSHNQLSGKIPASLARVNFNVIDLSRNKLEGDASVIFGGKKTTEIVDL 254
           FG F G+VP L+LSHNQLSG IP SL   +F  IDLSRNKL+GDAS++FG KKTT IVD+
Sbjct: 187 FGTFSGKVPSLFLSHNQLSGTIPKSLGNPDFYRIDLSRNKLQGDASILFGAKKTTWIVDI 246

Query: 255 SRNLLEFNLSKVVFPKSLTWLDLNHNRIFGGIPATVAKLELQLLNVSYNRLCGRIPVGGR 314
           SRN+ +F+LSKV   K+L  LD+NHN I G IPA  +K   QLLNVSYNRLCGRIP G  
Sbjct: 247 SRNMFQFDLSKVKLAKTLNNLDMNHNGITGSIPAEWSKAYFQLLNVSYNRLCGRIPKGEY 306

Query: 315 LQSFDLYSYFHNKCLCGKPLGSCK 336
           +Q FD YS+FHNKCLCG PL SCK
Sbjct: 307 IQRFDSYSFFHNKCLCGAPLPSCK 330

BLAST of Sgr017320 vs. ExPASy Swiss-Prot
Match: Q9LH52 (Leucine-rich repeat protein FLOR 1 OS=Arabidopsis thaliana OX=3702 GN=FLR1 PE=1 SV=1)

HSP 1 Score: 334.3 bits (856), Expect = 1.5e-90
Identity = 165/321 (51.40%), Postives = 218/321 (67.91%), Query Frame = 0

Query: 15  LFLFLFLFLFTISVAERCNPDDKKVLLKIKKAFNDPYILASWKPEEDCCT-WYCVDCHPS 74
           L +F  +   T+  +  C  +DK  LL+IKKA  +P +L+SW P  DCCT W  V+C  +
Sbjct: 7   LSIFFSILFITLPSSYSCTENDKNALLQIKKALGNPPLLSSWNPRTDCCTGWTGVEC--T 66

Query: 75  SHRIISLTIFSGELSGQIPPEVGDLPFLETLMFHKLSNLTGPIQPAIAKLRHLKSLDLSW 134
           + R+  L++ SGE+SGQI  ++GDL  L TL F  L +LTG I   I KL++L +L L  
Sbjct: 67  NRRVTGLSVTSGEVSGQISYQIGDLVDLRTLDFSYLPHLTGNIPRTITKLKNLNTLYLKH 126

Query: 135 TNLSGPIPGFLGSLTGLTYLDLSFNHLTGPIPSSLANLPNLGALHLDRNKLTGPIPDSFG 194
           T+LSGPIP ++  L  LT+LDLSFN  TGPIP SL+ +P L A+ ++ NKLTG IP+SFG
Sbjct: 127 TSLSGPIPDYISELKSLTFLDLSFNQFTGPIPGSLSQMPKLEAIQINDNKLTGSIPNSFG 186

Query: 195 QFKGQVPYLYLSHNQLSGKIPASLARVNFNVIDLSRNKLEGDASVIFGGKKTTEIVDLSR 254
            F G VP LYLS+N+LSGKIP SL++ +FN +DLS N  EGDA + FG  KTT  VDLSR
Sbjct: 187 SFVGNVPNLYLSNNKLSGKIPESLSKYDFNAVDLSGNGFEGDAFMFFGRNKTTVRVDLSR 246

Query: 255 NLLEFNLSKVVFPKSLTWLDLNHNRIFGGIPATVAKLELQLLNVSYNRLCGRIPVGGRLQ 314
           N+  F+L KV F +S+  LDL+ N I+G IP  + KL L+  NVS N LCG+IP GG LQ
Sbjct: 247 NMFNFDLVKVKFARSIVSLDLSQNHIYGKIPPALTKLHLEHFNVSDNHLCGKIPSGGLLQ 306

Query: 315 SFDLYSYFHNKCLCGKPLGSC 335
           +F+  ++ HN CLCG PL +C
Sbjct: 307 TFEPSAFAHNICLCGTPLKAC 325

BLAST of Sgr017320 vs. ExPASy TrEMBL
Match: A0A1W6IYG8 (Pgip1 OS=Momordica charantia OX=3673 GN=LOC111019559 PE=2 SV=1)

HSP 1 Score: 558.1 bits (1437), Expect = 2.4e-155
Identity = 264/321 (82.24%), Postives = 285/321 (88.79%), Query Frame = 0

Query: 16  FLFLFLFLFTISVAERCNPDDKKVLLKIKKAFNDPYILASWKPEEDCCTWYCVDCHPSSH 75
           F FL     +I  AE CNP+DKKVLLKIKK+FN+PYIL SWKPEEDCC+WYCV CHP+SH
Sbjct: 8   FFFLLFISSSICFAELCNPNDKKVLLKIKKSFNNPYILTSWKPEEDCCSWYCVQCHPTSH 67

Query: 76  RIISLTIFSGE-LSGQIPPEVGDLPFLETLMFHKLSNLTGPIQPAIAKLRHLKSLDLSWT 135
           RIISLTIF+ + LSGQIPP VGDLPFLETLM HKL NLTGPI  A+A L +LKSLDLSW 
Sbjct: 68  RIISLTIFADDKLSGQIPPVVGDLPFLETLMLHKLPNLTGPIPAAVANLHNLKSLDLSWN 127

Query: 136 NLSGPIPGFLGSLTGLTYLDLSFNHLTGPIPSSLANLPNLGALHLDRNKLTGPIPDSFGQ 195
            LSGP+P FLGSL+ LTYLDLSFN+LTG IPSSLANL  LGALHLDRNKLTGPIP SFG 
Sbjct: 128 GLSGPVPEFLGSLSSLTYLDLSFNNLTGSIPSSLANLQRLGALHLDRNKLTGPIPHSFGY 187

Query: 196 FKGQVPYLYLSHNQLSGKIPASLARVNFNVIDLSRNKLEGDASVIFGGKKTTEIVDLSRN 255
           FKG++PYLYLSHNQLSGKIPASL RVNFNVIDLSRNKLEGDASVIFGG KTTEIVDLSRN
Sbjct: 188 FKGKIPYLYLSHNQLSGKIPASLGRVNFNVIDLSRNKLEGDASVIFGGNKTTEIVDLSRN 247

Query: 256 LLEFNLSKVVFPKSLTWLDLNHNRIFGGIPATVAKLELQLLNVSYNRLCGRIPVGGRLQS 315
           LLEF+LSKVVFP+S+TWLDLNHN+IFGGIP  V +LELQLLNVSYNRLCGRIPVGGRLQ 
Sbjct: 248 LLEFDLSKVVFPRSVTWLDLNHNKIFGGIPEKVVELELQLLNVSYNRLCGRIPVGGRLQR 307

Query: 316 FDLYSYFHNKCLCGKPLGSCK 336
           FD+Y YFHNKCLCGKPLGSCK
Sbjct: 308 FDVYEYFHNKCLCGKPLGSCK 328

BLAST of Sgr017320 vs. ExPASy TrEMBL
Match: A0A6J1ELI8 (polygalacturonase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111435518 PE=4 SV=1)

HSP 1 Score: 538.5 bits (1386), Expect = 2.0e-149
Identity = 258/326 (79.14%), Postives = 283/326 (86.81%), Query Frame = 0

Query: 11  PATFLFLFLFLFLFTISVAERCNPDDKKVLLKIKKAFNDPYILASWKPEEDCCTWYCVDC 70
           P+TFLF+F FLF FTIS AE C+P+DKK LL IKKAFN+PYIL SWKPEEDCCTWYCV+C
Sbjct: 5   PSTFLFIF-FLF-FTISFAELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCTWYCVEC 64

Query: 71  HPSSHRIISLTIFSG-ELSGQIPPEVGDLPFLETLMFHKLSNLTGPIQPAIAKLRHLKSL 130
              SHRI+SLTIF+   LSGQIPP VGDLP+LE LMFHKL NLTGPIQPAIAKL +LK L
Sbjct: 65  DRKSHRIVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLHNLKYL 124

Query: 131 DLSWTNLSGPIPGFLGSLTGLTYLDLSFNHLTGPIPSSLANLPNLGALHLDRNKLTGPIP 190
           DLSW +L+G IP FL SLT L  LDLSFN LTG IPSSLANL  LG LHLDRNKLTGPIP
Sbjct: 125 DLSWNSLTGSIPNFLSSLTNLDLLDLSFNRLTGSIPSSLANLQRLGTLHLDRNKLTGPIP 184

Query: 191 DSFGQFKGQVPYLYLSHNQLSGKIPASLARVNFNVIDLSRNKLEGDASVIFGGKKTTEIV 250
           DSFG FKG++PYLYLSHNQLSGKIP SL RV+FNVIDLSRN+LEGD SVIFGGKKTTEIV
Sbjct: 185 DSFGNFKGKIPYLYLSHNQLSGKIPTSLERVDFNVIDLSRNRLEGDGSVIFGGKKTTEIV 244

Query: 251 DLSRNLLEFNLSKVVFPKSLTWLDLNHNRIFGGIPATVAKLELQLLNVSYNRLCGRIPVG 310
           DLSRNLLEF++SKVV P+SLTWLDLNHN+IFG IP     L+LQLLNVSYN+LCGRIPVG
Sbjct: 245 DLSRNLLEFDMSKVVLPRSLTWLDLNHNKIFGEIPVMAVNLQLQLLNVSYNKLCGRIPVG 304

Query: 311 GRLQSFDLYSYFHNKCLCGKPLGSCK 336
           G+LQSFD+YSY +NKCLCGKPLG CK
Sbjct: 305 GKLQSFDVYSYSNNKCLCGKPLGKCK 328

BLAST of Sgr017320 vs. ExPASy TrEMBL
Match: A0A6J1HW96 (polygalacturonase inhibitor-like OS=Cucurbita maxima OX=3661 GN=LOC111467229 PE=4 SV=1)

HSP 1 Score: 534.6 bits (1376), Expect = 2.9e-148
Identity = 256/326 (78.53%), Postives = 281/326 (86.20%), Query Frame = 0

Query: 11  PATFLFLFLFLFLFTISVAERCNPDDKKVLLKIKKAFNDPYILASWKPEEDCCTWYCVDC 70
           P+TFL +F FLF FTIS AE C+P+DKK LL IKKAFN+PYIL SWKPEEDCCTWYCV+C
Sbjct: 5   PSTFLLIF-FLF-FTISFAELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCTWYCVEC 64

Query: 71  HPSSHRIISLTIFSG-ELSGQIPPEVGDLPFLETLMFHKLSNLTGPIQPAIAKLRHLKSL 130
             +SHRI+SLTIF+   LSGQIPP VGDLP+LE LMFHKL NLTGPIQPAIAKL +LK L
Sbjct: 65  DRNSHRIVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLHNLKYL 124

Query: 131 DLSWTNLSGPIPGFLGSLTGLTYLDLSFNHLTGPIPSSLANLPNLGALHLDRNKLTGPIP 190
           DLSW  L+G IP FL SLT L  LDLSFN LTG IPSSLANL  LG LHLDRNKLTGPIP
Sbjct: 125 DLSWNTLTGSIPNFLSSLTNLELLDLSFNRLTGSIPSSLANLQRLGTLHLDRNKLTGPIP 184

Query: 191 DSFGQFKGQVPYLYLSHNQLSGKIPASLARVNFNVIDLSRNKLEGDASVIFGGKKTTEIV 250
           DSFG FKG++PYLYLSHNQLSGKIP S  RV+FNVIDLSRN+LEGD SVIFGGKKTTEIV
Sbjct: 185 DSFGNFKGKIPYLYLSHNQLSGKIPTSFERVDFNVIDLSRNRLEGDGSVIFGGKKTTEIV 244

Query: 251 DLSRNLLEFNLSKVVFPKSLTWLDLNHNRIFGGIPATVAKLELQLLNVSYNRLCGRIPVG 310
           DLSRNLLEF++SKVV P+SLTWLDLNHN+IFG IP     L+LQLLNVSYN+LCGRIPVG
Sbjct: 245 DLSRNLLEFDMSKVVLPRSLTWLDLNHNKIFGKIPVMAVNLQLQLLNVSYNKLCGRIPVG 304

Query: 311 GRLQSFDLYSYFHNKCLCGKPLGSCK 336
           G+LQSFD+YSY +NKCLCGKPLG CK
Sbjct: 305 GKLQSFDVYSYSNNKCLCGKPLGKCK 328

BLAST of Sgr017320 vs. ExPASy TrEMBL
Match: Q7X9Q7 (Polygalacturonase-inhibiting protein OS=Cucumis melo OX=3656 GN=LOC103499550 PE=2 SV=1)

HSP 1 Score: 534.3 bits (1375), Expect = 3.8e-148
Identity = 252/320 (78.75%), Postives = 275/320 (85.94%), Query Frame = 0

Query: 17  LFLFLFLFTISVAERCNPDDKKVLLKIKKAFNDPYILASWKPEEDCCTWYCVDCHPSSHR 76
           L L  F FT+S AE C+P+DK+VLL IKKAFN+PYIL SWKPEEDCCTWYCV+C   SHR
Sbjct: 7   LLLLFFFFTVSFAELCHPNDKEVLLNIKKAFNNPYILTSWKPEEDCCTWYCVECDLKSHR 66

Query: 77  IISLTIFS-GELSGQIPPEVGDLPFLETLMFHKLSNLTGPIQPAIAKLRHLKSLDLSWTN 136
           II+LTIF+  ELSG IPP VGDLPFLE LMFHKL NLTGPI P IAKL +LK LDLSW  
Sbjct: 67  IIALTIFADDELSGPIPPFVGDLPFLENLMFHKLPNLTGPIPPTIAKLHNLKYLDLSWNG 126

Query: 137 LSGPIPGFLGSLTGLTYLDLSFNHLTGPIPSSLANLPNLGALHLDRNKLTGPIPDSFGQF 196
           LSGPIP FLGSL+ L  LDLSFN  TG IPSSLANL  LG LHLDRNKLTGPIPDSFG F
Sbjct: 127 LSGPIPSFLGSLSNLDILDLSFNRFTGSIPSSLANLRRLGTLHLDRNKLTGPIPDSFGNF 186

Query: 197 KGQVPYLYLSHNQLSGKIPASLARVNFNVIDLSRNKLEGDASVIFGGKKTTEIVDLSRNL 256
           KG+VPYLYLSHNQLSGKIP S+ +V+FN IDLSRNKL GD S+IFG KKTTEIVDLSRNL
Sbjct: 187 KGKVPYLYLSHNQLSGKIPTSMGKVDFNYIDLSRNKLVGDGSLIFGSKKTTEIVDLSRNL 246

Query: 257 LEFNLSKVVFPKSLTWLDLNHNRIFGGIPATVAKLELQLLNVSYNRLCGRIPVGGRLQSF 316
           LEFN+SKVVFP++LTWLDLNHN+IFG IP  V KLELQ+LNVSYN LCGRIP+GG+LQSF
Sbjct: 247 LEFNISKVVFPRTLTWLDLNHNKIFGEIPTGVVKLELQMLNVSYNALCGRIPMGGKLQSF 306

Query: 317 DLYSYFHNKCLCGKPLGSCK 336
           D+YSYFHNKCLCGKPLGSCK
Sbjct: 307 DVYSYFHNKCLCGKPLGSCK 326

BLAST of Sgr017320 vs. ExPASy TrEMBL
Match: A0A5D3BX47 (Polygalacturonase inhibitor-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold4559G00050 PE=4 SV=1)

HSP 1 Score: 534.3 bits (1375), Expect = 3.8e-148
Identity = 252/320 (78.75%), Postives = 275/320 (85.94%), Query Frame = 0

Query: 17  LFLFLFLFTISVAERCNPDDKKVLLKIKKAFNDPYILASWKPEEDCCTWYCVDCHPSSHR 76
           L L  F FT+S AE C+P+DK+VLL IKKAFN+PYIL SWKPEEDCCTWYCV+C   SHR
Sbjct: 7   LLLLFFFFTVSFAELCHPNDKEVLLNIKKAFNNPYILTSWKPEEDCCTWYCVECDLKSHR 66

Query: 77  IISLTIFS-GELSGQIPPEVGDLPFLETLMFHKLSNLTGPIQPAIAKLRHLKSLDLSWTN 136
           II+LTIF+  ELSG IPP VGDLPFLE LMFHKL NLTGPI P IAKL +LK LDLSW  
Sbjct: 67  IIALTIFADDELSGPIPPFVGDLPFLENLMFHKLPNLTGPIPPTIAKLHNLKYLDLSWNG 126

Query: 137 LSGPIPGFLGSLTGLTYLDLSFNHLTGPIPSSLANLPNLGALHLDRNKLTGPIPDSFGQF 196
           LSGPIP FLGSL+ L  LDLSFN  TG IPSSLANL  LG LHLDRNKLTGPIPDSFG F
Sbjct: 127 LSGPIPSFLGSLSNLDILDLSFNRFTGSIPSSLANLRRLGTLHLDRNKLTGPIPDSFGNF 186

Query: 197 KGQVPYLYLSHNQLSGKIPASLARVNFNVIDLSRNKLEGDASVIFGGKKTTEIVDLSRNL 256
           KG+VPYLYLSHNQLSGKIP S+ +V+FN IDLSRNKL GD S+IFG KKTTEIVDLSRNL
Sbjct: 187 KGKVPYLYLSHNQLSGKIPTSMGKVDFNYIDLSRNKLVGDGSLIFGSKKTTEIVDLSRNL 246

Query: 257 LEFNLSKVVFPKSLTWLDLNHNRIFGGIPATVAKLELQLLNVSYNRLCGRIPVGGRLQSF 316
           LEFN+SKVVFP++LTWLDLNHN+IFG IP  V KLELQ+LNVSYN LCGRIP+GG+LQSF
Sbjct: 247 LEFNISKVVFPRTLTWLDLNHNKIFGEIPTGVVKLELQMLNVSYNALCGRIPMGGKLQSF 306

Query: 317 DLYSYFHNKCLCGKPLGSCK 336
           D+YSYFHNKCLCGKPLGSCK
Sbjct: 307 DVYSYFHNKCLCGKPLGSCK 326

BLAST of Sgr017320 vs. TAIR 10
Match: AT5G06860.1 (polygalacturonase inhibiting protein 1 )

HSP 1 Score: 434.5 bits (1116), Expect = 7.8e-122
Identity = 206/326 (63.19%), Postives = 248/326 (76.07%), Query Frame = 0

Query: 12  ATFLFLFLFLFLFTISVAERCNPDDKKVLLKIKKAFNDPYILASWKPEEDCCTWYCVDCH 71
           AT   LFLF FL T    + CN +DK  LLKIKK+ N+PY LASW P+ DCC+WYC++C 
Sbjct: 5   ATLCLLFLFTFLTTCLSKDLCNQNDKNTLLKIKKSLNNPYHLASWDPQTDCCSWYCLECG 64

Query: 72  PS--SHRIISLTIFSGELSGQIPPEVGDLPFLETLMFHKLSNLTGPIQPAIAKLRHLKSL 131
            +  +HR+ +LTIFSG++SGQIP EVGDLP+LETL+F KLSNLTG IQP IAKL++L+ L
Sbjct: 65  DATVNHRVTALTIFSGQISGQIPAEVGDLPYLETLVFRKLSNLTGTIQPTIAKLKNLRML 124

Query: 132 DLSWTNLSGPIPGFLGSLTGLTYLDLSFNHLTGPIPSSLANLPNLGALHLDRNKLTGPIP 191
            LSWTNL+GPIP F+  L  L +L+LSFN L+G IPSSL+ LP + AL L RNKLTG IP
Sbjct: 125 RLSWTNLTGPIPDFISQLKNLEFLELSFNDLSGSIPSSLSTLPKILALELSRNKLTGSIP 184

Query: 192 DSFGQFKGQVPYLYLSHNQLSGKIPASLARVNFNVIDLSRNKLEGDASVIFGGKKTTEIV 251
           +SFG F G VP L LSHNQLSG IP SL  ++FN IDLSRNKL+GDAS++FG  KTT  +
Sbjct: 185 ESFGSFPGTVPDLRLSHNQLSGPIPKSLGNIDFNRIDLSRNKLQGDASMLFGSNKTTWSI 244

Query: 252 DLSRNLLEFNLSKVVFPKSLTWLDLNHNRIFGGIPATVAKLELQLLNVSYNRLCGRIPVG 311
           DLSRN+ +F++SKV  PK+L  LDLNHN I G IP    +  LQ  NVSYN+LCG IP G
Sbjct: 245 DLSRNMFQFDISKVDIPKTLGILDLNHNGITGNIPVQWTEAPLQFFNVSYNKLCGHIPTG 304

Query: 312 GRLQSFDLYSYFHNKCLCGKPLGSCK 336
           G+LQ+FD YSYFHNKCLCG PL  CK
Sbjct: 305 GKLQTFDSYSYFHNKCLCGAPLEICK 330

BLAST of Sgr017320 vs. TAIR 10
Match: AT5G06870.1 (polygalacturonase inhibiting protein 2 )

HSP 1 Score: 423.3 bits (1087), Expect = 1.8e-118
Identity = 206/324 (63.58%), Postives = 247/324 (76.23%), Query Frame = 0

Query: 15  LFLFLFLFLFTISVA-ERCNPDDKKVLLKIKKAFNDPYILASWKPEEDCCTWYCVDCHPS 74
           LFL L   L T S+A + C+ DDK  LLKIKK+ N+PY LASW P+ DCC+WYC++C  +
Sbjct: 7   LFLLLSTLLLTTSLAKDLCHKDDKTTLLKIKKSLNNPYHLASWDPKTDCCSWYCLECGDA 66

Query: 75  --SHRIISLTIFSGELSGQIPPEVGDLPFLETLMFHKLSNLTGPIQPAIAKLRHLKSLDL 134
             +HR+ SL I  GE+SGQIPPEVGDLP+L +L+F KL+NLTG IQP IAKL++L  L L
Sbjct: 67  TVNHRVTSLIIQDGEISGQIPPEVGDLPYLTSLIFRKLTNLTGHIQPTIAKLKNLTFLRL 126

Query: 135 SWTNLSGPIPGFLGSLTGLTYLDLSFNHLTGPIPSSLANLPNLGALHLDRNKLTGPIPDS 194
           SWTNL+GP+P FL  L  L Y+DLSFN L+G IPSSL++L  L  L L RNKLTGPIP+S
Sbjct: 127 SWTNLTGPVPEFLSQLKNLEYIDLSFNDLSGSIPSSLSSLRKLEYLELSRNKLTGPIPES 186

Query: 195 FGQFKGQVPYLYLSHNQLSGKIPASLARVNFNVIDLSRNKLEGDASVIFGGKKTTEIVDL 254
           FG F G+VP L+LSHNQLSG IP SL   +F  IDLSRNKL+GDAS++FG KKTT IVD+
Sbjct: 187 FGTFSGKVPSLFLSHNQLSGTIPKSLGNPDFYRIDLSRNKLQGDASILFGAKKTTWIVDI 246

Query: 255 SRNLLEFNLSKVVFPKSLTWLDLNHNRIFGGIPATVAKLELQLLNVSYNRLCGRIPVGGR 314
           SRN+ +F+LSKV   K+L  LD+NHN I G IPA  +K   QLLNVSYNRLCGRIP G  
Sbjct: 247 SRNMFQFDLSKVKLAKTLNNLDMNHNGITGSIPAEWSKAYFQLLNVSYNRLCGRIPKGEY 306

Query: 315 LQSFDLYSYFHNKCLCGKPLGSCK 336
           +Q FD YS+FHNKCLCG PL SCK
Sbjct: 307 IQRFDSYSFFHNKCLCGAPLPSCK 330

BLAST of Sgr017320 vs. TAIR 10
Match: AT3G12145.1 (Leucine-rich repeat (LRR) family protein )

HSP 1 Score: 334.3 bits (856), Expect = 1.1e-91
Identity = 165/321 (51.40%), Postives = 218/321 (67.91%), Query Frame = 0

Query: 15  LFLFLFLFLFTISVAERCNPDDKKVLLKIKKAFNDPYILASWKPEEDCCT-WYCVDCHPS 74
           L +F  +   T+  +  C  +DK  LL+IKKA  +P +L+SW P  DCCT W  V+C  +
Sbjct: 7   LSIFFSILFITLPSSYSCTENDKNALLQIKKALGNPPLLSSWNPRTDCCTGWTGVEC--T 66

Query: 75  SHRIISLTIFSGELSGQIPPEVGDLPFLETLMFHKLSNLTGPIQPAIAKLRHLKSLDLSW 134
           + R+  L++ SGE+SGQI  ++GDL  L TL F  L +LTG I   I KL++L +L L  
Sbjct: 67  NRRVTGLSVTSGEVSGQISYQIGDLVDLRTLDFSYLPHLTGNIPRTITKLKNLNTLYLKH 126

Query: 135 TNLSGPIPGFLGSLTGLTYLDLSFNHLTGPIPSSLANLPNLGALHLDRNKLTGPIPDSFG 194
           T+LSGPIP ++  L  LT+LDLSFN  TGPIP SL+ +P L A+ ++ NKLTG IP+SFG
Sbjct: 127 TSLSGPIPDYISELKSLTFLDLSFNQFTGPIPGSLSQMPKLEAIQINDNKLTGSIPNSFG 186

Query: 195 QFKGQVPYLYLSHNQLSGKIPASLARVNFNVIDLSRNKLEGDASVIFGGKKTTEIVDLSR 254
            F G VP LYLS+N+LSGKIP SL++ +FN +DLS N  EGDA + FG  KTT  VDLSR
Sbjct: 187 SFVGNVPNLYLSNNKLSGKIPESLSKYDFNAVDLSGNGFEGDAFMFFGRNKTTVRVDLSR 246

Query: 255 NLLEFNLSKVVFPKSLTWLDLNHNRIFGGIPATVAKLELQLLNVSYNRLCGRIPVGGRLQ 314
           N+  F+L KV F +S+  LDL+ N I+G IP  + KL L+  NVS N LCG+IP GG LQ
Sbjct: 247 NMFNFDLVKVKFARSIVSLDLSQNHIYGKIPPALTKLHLEHFNVSDNHLCGKIPSGGLLQ 306

Query: 315 SFDLYSYFHNKCLCGKPLGSC 335
           +F+  ++ HN CLCG PL +C
Sbjct: 307 TFEPSAFAHNICLCGTPLKAC 325

BLAST of Sgr017320 vs. TAIR 10
Match: AT5G12940.1 (Leucine-rich repeat (LRR) family protein )

HSP 1 Score: 193.7 bits (491), Expect = 2.3e-49
Identity = 121/356 (33.99%), Postives = 175/356 (49.16%), Query Frame = 0

Query: 21  LFLFTISVAERCNPDDKKVLLKIKKAFNDPYI--LASWKPEEDCCTWYCVDCHPSSHRII 80
           +FL   +    C P D+  LL+ +   N+PYI    +WK  + C  WY V C P++ R+ 
Sbjct: 16  VFLLLSTTVHSCLPSDRAALLEFRAKLNEPYIGVFNTWKGLDCCKGWYGVSCDPNTRRVA 75

Query: 81  SLTI-------------FSGELSGQIPPEVGDLPFLETLMFHKLSNLTGPIQPAIAKLRH 140
            +T+              SG ++G I P +  L  L  ++      ++G I   I  L  
Sbjct: 76  GITLRGESEDPLFQKAKRSGLMTGSISPSICKLTRLSGIIIADWKGISGVIPSCIENLPF 135

Query: 141 LKSLDLSWTNLSGPIPGFLGSLTGLTYLDLSFNHLTGPIPSSLANLPNLGALHLDRNKLT 200
           L+ LDL     SG IP  +G L  L  L+L+ NHL G IP S+  L +L  L L  N ++
Sbjct: 136 LRHLDLVGNKFSGVIPANIGKLLRLKVLNLADNHLYGVIPPSITRLVSLSHLDLRNNNIS 195

Query: 201 GPIPDSFGQFKGQVPYLYLSHNQLSGKIPASLARV-NFNVIDLSRNKLEGDASVIFGGKK 260
           G IP   G+ K  V  + LS N++SG+IP SL R+     ++LS N+L G     FG   
Sbjct: 196 GVIPRDIGRLK-MVSRVLLSGNKISGQIPDSLTRIYRLADLELSMNRLTGPIPASFGKMS 255

Query: 261 TTEIVDLSRNLL--------------EFNLSKVVFPKSL----------TWLDLNHNRIF 320
               ++L  NL+                NLS  +   S+          T LDL +NR+ 
Sbjct: 256 VLATLNLDGNLISGMIPGSLLASSISNLNLSGNLITGSIPNTFGPRSYFTVLDLANNRLQ 315

Query: 321 GGIPATV-AKLELQLLNVSYNRLCGRIPVGGRLQSFDLYSYFHNKCLCGKPLGSCK 336
           G IPA++ A   +  L+VS+N LCG+IP+G      D  S+ +N CLCGKPLG+CK
Sbjct: 316 GPIPASITAASFIGHLDVSHNHLCGKIPMGSPFDHLDATSFAYNACLCGKPLGNCK 370

BLAST of Sgr017320 vs. TAIR 10
Match: AT3G12610.1 (Leucine-rich repeat (LRR) family protein )

HSP 1 Score: 191.8 bits (486), Expect = 8.8e-49
Identity = 120/361 (33.24%), Postives = 179/361 (49.58%), Query Frame = 0

Query: 14  FLFLFLFLFLFTISVAERCNPDDKKVLLKIKKAFNDPY--ILASWKPEEDCC-TWYCVDC 73
           F  L   +F+  ISV   C+P D+  L   K + ++P   I  +W    DCC  WY + C
Sbjct: 9   FSSLLAVVFISVISVVRCCSPKDQTALNAFKSSLSEPNLGIFNTWSENTDCCKEWYGISC 68

Query: 74  HPSSHRIISLTI-------------FSGELSGQIPPEVGDLPFLETLMFHKLSNLTGPIQ 133
            P S R+  +++              SG +SG I P V DL  L +L+      +TG I 
Sbjct: 69  DPDSGRVTDISLRGESEDAIFQKAGRSGYMSGSIDPAVCDLTALTSLVLADWKGITGEIP 128

Query: 134 PAIAKLRHLKSLDLSWTNLSGPIPGFLGSLTGLTYLDLSFNHLTGPIPSSLANLPNLGAL 193
           P I  L  L+ LDL+   ++G IP  +G L+ L  L+L+ N ++G IP+SL +L  L  L
Sbjct: 129 PCITSLASLRILDLAGNKITGEIPAEIGKLSKLAVLNLAENQMSGEIPASLTSLIELKHL 188

Query: 194 HLDRNKLTGPIPDSFGQFKGQVPYLYLSHNQLSGKIPASLARV-NFNVIDLSRNKLEGDA 253
            L  N +TG IP  FG  K  +  + L  N+L+G IP S++ +     +DLS+N +EG  
Sbjct: 189 ELTENGITGVIPADFGSLK-MLSRVLLGRNELTGSIPESISGMERLADLDLSKNHIEGPI 248

Query: 254 SVIFGGKKT------------------------TEIVDLSRNLLEFNLSKVVFPKS-LTW 313
               G  K                          ++ +LSRN LE  +  V   K+ L  
Sbjct: 249 PEWMGNMKVLSLLNLDCNSLTGPIPGSLLSNSGLDVANLSRNALEGTIPDVFGSKTYLVS 308

Query: 314 LDLNHNRIFGGIPATVAKLE-LQLLNVSYNRLCGRIPVGGRLQSFDLYSYFHNKCLCGKP 332
           LDL+HN + G IP +++  + +  L++S+N+LCGRIP G      +  S+  N+CLCG P
Sbjct: 309 LDLSHNSLSGRIPDSLSSAKFVGHLDISHNKLCGRIPTGFPFDHLEATSFSDNQCLCGGP 368

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022151650.15.0e-15582.24polygalacturonase inhibitor-like [Momordica charantia] >ARM53420.1 pgip1 [Momord... [more]
XP_038880971.11.8e-14979.50polygalacturonase inhibitor-like [Benincasa hispida][more]
XP_022928674.14.1e-14979.14polygalacturonase inhibitor-like [Cucurbita moschata] >XP_023543570.1 polygalact... [more]
KAG6603233.19.2e-14979.14Polygalacturonase inhibitor, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022967838.15.9e-14878.53polygalacturonase inhibitor-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q050913.5e-13572.00Polygalacturonase inhibitor OS=Pyrus communis OX=23211 GN=PGIP PE=1 SV=1[more]
A7PW812.1e-13271.20Polygalacturonase inhibitor OS=Vitis vinifera OX=29760 GN=pgip PE=1 SV=1[more]
Q9M5J91.1e-12063.19Polygalacturonase inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=PGIP1 PE=1 SV=1[more]
Q9M5J82.5e-11763.58Polygalacturonase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PGIP2 PE=2 SV=2[more]
Q9LH521.5e-9051.40Leucine-rich repeat protein FLOR 1 OS=Arabidopsis thaliana OX=3702 GN=FLR1 PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A1W6IYG82.4e-15582.24Pgip1 OS=Momordica charantia OX=3673 GN=LOC111019559 PE=2 SV=1[more]
A0A6J1ELI82.0e-14979.14polygalacturonase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111435518 P... [more]
A0A6J1HW962.9e-14878.53polygalacturonase inhibitor-like OS=Cucurbita maxima OX=3661 GN=LOC111467229 PE=... [more]
Q7X9Q73.8e-14878.75Polygalacturonase-inhibiting protein OS=Cucumis melo OX=3656 GN=LOC103499550 PE=... [more]
A0A5D3BX473.8e-14878.75Polygalacturonase inhibitor-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
Match NameE-valueIdentityDescription
AT5G06860.17.8e-12263.19polygalacturonase inhibiting protein 1 [more]
AT5G06870.11.8e-11863.58polygalacturonase inhibiting protein 2 [more]
AT3G12145.11.1e-9151.40Leucine-rich repeat (LRR) family protein [more]
AT5G12940.12.3e-4933.99Leucine-rich repeat (LRR) family protein [more]
AT3G12610.18.8e-4933.24Leucine-rich repeat (LRR) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001611Leucine-rich repeatPFAMPF00560LRR_1coord: 268..289
e-value: 1.7
score: 9.5
IPR001611Leucine-rich repeatPFAMPF13855LRR_8coord: 124..184
e-value: 4.1E-9
score: 36.1
IPR013210Leucine-rich repeat-containing N-terminal, plant-typePFAMPF08263LRRNT_2coord: 34..70
e-value: 8.6E-10
score: 38.8
IPR032675Leucine-rich repeat domain superfamilyGENE3D3.80.10.10Ribonuclease Inhibitorcoord: 31..335
e-value: 1.0E-86
score: 292.9
NoneNo IPR availablePANTHERPTHR48059:SF10POLYGALACTURONASE INHIBITOR 2coord: 13..334
NoneNo IPR availablePANTHERPTHR48059POLYGALACTURONASE INHIBITOR 1coord: 13..334
NoneNo IPR availableSUPERFAMILY52058L domain-likecoord: 31..333

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr017320.1Sgr017320.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043086 negative regulation of catalytic activity
molecular_function GO:0090353 polygalacturonase inhibitor activity
molecular_function GO:0005515 protein binding