Sgr017073 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr017073 vs. NCBI nr
Match: XP_023549997.1 (aspartyl protease family protein 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 716.1 bits (1847), Expect = 1.9e-202
Identity = 355/457 (77.68%), Postives = 394/457 (86.21%), Query Frame = 0

Query: 1   MLSLSPCFPLLLLLFFFTDFCSIPKTAATEYLKLPLLHKNPFSSPSEALSFDTHRLSVLF 60
           MLS+SP F  +LLLF   D   +   AA +YLKLPLLHKNPFSSPS+ALS DTHRLS+LF
Sbjct: 5   MLSVSPFFHFILLLFSLADL--LNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSLLF 64

Query: 61  SALHGR--NTLKSPVISGASTGSGQYFVDLRLGTPPQSLLLVADTGSDLVWVKCSACRNC 120
           SAL  R   TLKSP+ISGASTGSGQYFVDLR+GTPPQSLLLVADTGSDLVWVKCSACRNC
Sbjct: 65  SALRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNC 124

Query: 121 SHHSPGSAFLPRHSSTFSPYHCYDSSCHLVTHPDHRVCNHTRLHTPCPYQYSYADGSLSN 180
           SHH P SAFLPRHSS+FSP+HC+D  C L+ H    +CNHTRLH+PC + Y+YADGS S+
Sbjct: 125 SHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGSTSS 184

Query: 181 GFFSKEATTLKTVSGREARIRNLSFGCGFRISGPSVSGLSFNGARGVMGLGRGPISFSSQ 240
           GFFSKE TTLKT+SG E R+++LSFGCGFRISGPSVSG  FNGARGVMGLGRGPISFS+Q
Sbjct: 185 GFFSKETTTLKTLSGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQ 244

Query: 241 LGRRFGNKFSYCLMDYTFSPPPTSYLMI-GGLRSPVVSNASRISYTPLQMNPLSPTFYYI 300
           LGRRFGNKFSYCLMDYT SPPPTSYLMI GGLRS  V+NA++ISYTPL +NPLSPTFYYI
Sbjct: 245 LGRRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNATKISYTPLLINPLSPTFYYI 304

Query: 301 GVKSITVDGVRLPINPAVWSIDELGNGGTVVDSGTTLTFLAEPAYDQVLTAFKRRVKLPK 360
            VKSITVDGV+LPINP VW+IDE GNGGTVVDSGTTLT+LAE AY +VL A ++RVKLP 
Sbjct: 305 AVKSITVDGVKLPINPTVWAIDEQGNGGTVVDSGTTLTYLAEEAYKEVLKAVRQRVKLPA 364

Query: 361 PAELTPGFDLCVNASGES-RVSLPRMSFRLAGGSVFSPPPRNYFLETEERVMCLAIRPVN 420
            AELTPGFDLCVN S ES R SLPR+ F+L  G+VF+PP RNYFLETEE VMCL+IR V 
Sbjct: 365 AAELTPGFDLCVNVSNESQRPSLPRVRFQLGNGAVFAPPARNYFLETEEGVMCLSIRAVE 424

Query: 421 SGNGFSIIGNLMQQGFLLEFDKDASRLGFSRRGCGLP 454
            GNGFS+IGNLMQQGFLLEFDK+ASRLGFSRRGCGLP
Sbjct: 425 GGNGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP 459

BLAST of Sgr017073 vs. NCBI nr
Match: XP_038907006.1 (aspartyl protease family protein 2 [Benincasa hispida])

HSP 1 Score: 715.3 bits (1845), Expect = 3.3e-202
Identity = 352/455 (77.36%), Postives = 392/455 (86.15%), Query Frame = 0

Query: 1   MLSLSPCFPLLLLLFFFTDFCSIPKTAATEYLKLPLLHKNPFSSPSEALSFDTHRLSVLF 60
           ML +SP F  + L FF T+F       A +YLKLPLLHK PFSSPS+ALS DTHRLS+LF
Sbjct: 1   MLPISPFFHFIFLFFFLTNF----NATAADYLKLPLLHKPPFSSPSQALSSDTHRLSLLF 60

Query: 61  SALHGRNTLKSPVISGASTGSGQYFVDLRLGTPPQSLLLVADTGSDLVWVKCSACRNCSH 120
           S      TLKSP+ISGASTGSGQYFVDLRLGTPPQSLLLVADTGSDLVWVKCSACRNCSH
Sbjct: 61  S--RPNPTLKSPLISGASTGSGQYFVDLRLGTPPQSLLLVADTGSDLVWVKCSACRNCSH 120

Query: 121 HSPGSAFLPRHSSTFSPYHCYDSSCHLVTHPDHRVCNHTRLHTPCPYQYSYADGSLSNGF 180
           H P +AFLPRHSS+FSP+HC+D  C L+ H    +CNHTR H+PC + YSYADGSLS+GF
Sbjct: 121 HPPSTAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRFHSPCRFLYSYADGSLSSGF 180

Query: 181 FSKEATTLKTVSGREARIRNLSFGCGFRISGPSVSGLSFNGARGVMGLGRGPISFSSQLG 240
           FSKE TTLKT+SG E  ++ LSFGCGFRISGPSVSG  F+GARGVMGLGRG ISFSSQLG
Sbjct: 181 FSKETTTLKTLSGSEIHLKGLSFGCGFRISGPSVSGAQFSGARGVMGLGRGSISFSSQLG 240

Query: 241 RRFGNKFSYCLMDYTFSPPPTSYLMI-GGLRSPVVSNASRISYTPLQMNPLSPTFYYIGV 300
           RRFGNKFSYCLMDYT SPPPTSYLMI GG RS  V+NA++ISYTPLQ+NPLSPTFYYI +
Sbjct: 241 RRFGNKFSYCLMDYTLSPPPTSYLMIGGGHRSLPVTNATKISYTPLQINPLSPTFYYIAI 300

Query: 301 KSITVDGVRLPINPAVWSIDELGNGGTVVDSGTTLTFLAEPAYDQVLTAFKRRVKLPKPA 360
            SITVDGV+LPINPAVW++D+ GNGGTVVDSGTTLT+LA+ AYD+VL A +RRVKLP  +
Sbjct: 301 HSITVDGVKLPINPAVWAMDKQGNGGTVVDSGTTLTYLAKAAYDEVLKAVRRRVKLPSAS 360

Query: 361 ELTPGFDLCVNASGES-RVSLPRMSFRLAGGSVFSPPPRNYFLETEERVMCLAIRPVNSG 420
           ELTPGFDLCVNAS  S R SLPR+ FRL GG+VF+PPPRNYFLETEERVMCLAIRPV+SG
Sbjct: 361 ELTPGFDLCVNASDSSRRPSLPRLRFRLGGGAVFAPPPRNYFLETEERVMCLAIRPVDSG 420

Query: 421 NGFSIIGNLMQQGFLLEFDKDASRLGFSRRGCGLP 454
           NGFS+IGNLMQQGFLLEFDKDA+RLGFSRRGCGLP
Sbjct: 421 NGFSVIGNLMQQGFLLEFDKDAARLGFSRRGCGLP 449

BLAST of Sgr017073 vs. NCBI nr
Match: XP_022928946.1 (aspartyl protease family protein 2-like [Cucurbita moschata])

HSP 1 Score: 714.1 bits (1842), Expect = 7.4e-202
Identity = 354/457 (77.46%), Postives = 393/457 (86.00%), Query Frame = 0

Query: 1   MLSLSPCFPLLLLLFFFTDFCSIPKTAATEYLKLPLLHKNPFSSPSEALSFDTHRLSVLF 60
           MLS+SP F L+LLLF   D   +   AA +YLKLPLLHKNPFSSPS+ALS DTHRLS+LF
Sbjct: 5   MLSVSPFFHLILLLFSLADL--LNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSLLF 64

Query: 61  SALHGR--NTLKSPVISGASTGSGQYFVDLRLGTPPQSLLLVADTGSDLVWVKCSACRNC 120
           SAL  R   TLKSP+ISGASTGSGQYFVDLR+GTPPQSLLLVADTGSDLVWVKCSACRNC
Sbjct: 65  SALRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNC 124

Query: 121 SHHSPGSAFLPRHSSTFSPYHCYDSSCHLVTHPDHRVCNHTRLHTPCPYQYSYADGSLSN 180
           SHH P SAFLPRHSS+FSP+HC+D  C L+ H    +CNHTRLH+PC + Y+YADGS S+
Sbjct: 125 SHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGSTSS 184

Query: 181 GFFSKEATTLKTVSGREARIRNLSFGCGFRISGPSVSGLSFNGARGVMGLGRGPISFSSQ 240
           GFFSKE TTLKT++G E R+++LSFGCGFRISGPSVSG  FNGARGVMGLGRGPISFS+Q
Sbjct: 185 GFFSKETTTLKTLTGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQ 244

Query: 241 LGRRFGNKFSYCLMDYTFSPPPTSYLMI-GGLRSPVVSNASRISYTPLQMNPLSPTFYYI 300
           LGRRFGNKFSYCLMDYT SPPPTSYLMI GGLR   V+NA++ISYTPL +NPLSPTFYYI
Sbjct: 245 LGRRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRRLPVTNATKISYTPLLINPLSPTFYYI 304

Query: 301 GVKSITVDGVRLPINPAVWSIDELGNGGTVVDSGTTLTFLAEPAYDQVLTAFKRRVKLPK 360
            VKSITVDGV+LPINP +W+IDE GNGGTVVDSGTTLT+LAE AY +VL A ++RVKLP 
Sbjct: 305 AVKSITVDGVKLPINPTLWAIDEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRVKLPA 364

Query: 361 PAELTPGFDLCVNASGES-RVSLPRMSFRLAGGSVFSPPPRNYFLETEERVMCLAIRPVN 420
            AELTPGFDLCVN S ES R SLPR+ F+L  G+VF PP RNYFLETEE VMCLAIR V 
Sbjct: 365 AAELTPGFDLCVNVSNESQRPSLPRVRFQLGNGAVFPPPARNYFLETEEGVMCLAIRAVE 424

Query: 421 SGNGFSIIGNLMQQGFLLEFDKDASRLGFSRRGCGLP 454
            GNGFS+IGNLMQQGFLLEFDK+ASRLGFSRRGCGLP
Sbjct: 425 GGNGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP 459

BLAST of Sgr017073 vs. NCBI nr
Match: KAG7017139.1 (Aspartic proteinase nepenthesin-2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 714.1 bits (1842), Expect = 7.4e-202
Identity = 354/457 (77.46%), Postives = 394/457 (86.21%), Query Frame = 0

Query: 1   MLSLSPCFPLLLLLFFFTDFCSIPKTAATEYLKLPLLHKNPFSSPSEALSFDTHRLSVLF 60
           MLS+SP F  +LLLF   D   +   AA +YLKLPLLHKNPFSSPS+ALS DTHRLS+LF
Sbjct: 1   MLSVSPFFHFILLLFSLADL--LNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSLLF 60

Query: 61  SALHGR--NTLKSPVISGASTGSGQYFVDLRLGTPPQSLLLVADTGSDLVWVKCSACRNC 120
           SAL  R   TLKSP+ISGASTGSGQYFVDLR+GTPPQSLLLVADTGSDLVWVKCSACRNC
Sbjct: 61  SALRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNC 120

Query: 121 SHHSPGSAFLPRHSSTFSPYHCYDSSCHLVTHPDHRVCNHTRLHTPCPYQYSYADGSLSN 180
           SHH P SAFLPRHSS+FSP+HC+D  C L+ H    +CNHTRLH+PC + Y+YADGS S+
Sbjct: 121 SHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGSTSS 180

Query: 181 GFFSKEATTLKTVSGREARIRNLSFGCGFRISGPSVSGLSFNGARGVMGLGRGPISFSSQ 240
           GFFSKE TTLKT+SG E R+++LSFGCGFRISGPSVSG  FNGARGVMGLGRGPISFS+Q
Sbjct: 181 GFFSKETTTLKTLSGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQ 240

Query: 241 LGRRFGNKFSYCLMDYTFSPPPTSYLMI-GGLRSPVVSNASRISYTPLQMNPLSPTFYYI 300
           LGRRFGNKFSYCLMDYT SPPPTSYLMI GGLRS  V+NA++ISYTPL +NPLSPTFYYI
Sbjct: 241 LGRRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNATKISYTPLLINPLSPTFYYI 300

Query: 301 GVKSITVDGVRLPINPAVWSIDELGNGGTVVDSGTTLTFLAEPAYDQVLTAFKRRVKLPK 360
            VKSITVDGV+LPINP VW+I+E GNGGTVVDSGTTLT+LAE AY +VL A ++RVKLP 
Sbjct: 301 AVKSITVDGVKLPINPIVWAINEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRVKLPA 360

Query: 361 PAELTPGFDLCVNASGES-RVSLPRMSFRLAGGSVFSPPPRNYFLETEERVMCLAIRPVN 420
            AELTPGFDLCVN S ES R SLPR+ F+L  G+VF+PP RNYFL+TEE VMCLAIR V 
Sbjct: 361 AAELTPGFDLCVNVSNESQRPSLPRVRFQLGNGAVFAPPARNYFLDTEEGVMCLAIRAVE 420

Query: 421 SGNGFSIIGNLMQQGFLLEFDKDASRLGFSRRGCGLP 454
            GNGFS+IGNLMQQGFLLEFDK+ASRLGFSRRGCGLP
Sbjct: 421 GGNGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP 455

BLAST of Sgr017073 vs. NCBI nr
Match: KAG6579697.1 (Aspartic proteinase NANA, chloroplast, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 714.1 bits (1842), Expect = 7.4e-202
Identity = 354/457 (77.46%), Postives = 394/457 (86.21%), Query Frame = 0

Query: 1   MLSLSPCFPLLLLLFFFTDFCSIPKTAATEYLKLPLLHKNPFSSPSEALSFDTHRLSVLF 60
           MLS+SP F  +LLLF   D   +   AA +YLKLPLLHKNPFSSPS+ALS DTHRLS+LF
Sbjct: 5   MLSVSPFFHFILLLFSLADL--LNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSLLF 64

Query: 61  SALHGR--NTLKSPVISGASTGSGQYFVDLRLGTPPQSLLLVADTGSDLVWVKCSACRNC 120
           SAL  R   TLKSP+ISGASTGSGQYFVDLR+GTPPQSLLLVADTGSDLVWVKCSACRNC
Sbjct: 65  SALRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNC 124

Query: 121 SHHSPGSAFLPRHSSTFSPYHCYDSSCHLVTHPDHRVCNHTRLHTPCPYQYSYADGSLSN 180
           SHH P SAFLPRHSS+FSP+HC+D  C L+ H    +CNHTRLH+PC + Y+YADGS S+
Sbjct: 125 SHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGSTSS 184

Query: 181 GFFSKEATTLKTVSGREARIRNLSFGCGFRISGPSVSGLSFNGARGVMGLGRGPISFSSQ 240
           GFFSKE TTLKT+SG E R+++LSFGCGFRISGPSVSG  FNGARGVMGLGRGPISFS+Q
Sbjct: 185 GFFSKETTTLKTLSGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQ 244

Query: 241 LGRRFGNKFSYCLMDYTFSPPPTSYLMI-GGLRSPVVSNASRISYTPLQMNPLSPTFYYI 300
           LGRRFGNKFSYCLMDYT SPPPTSYLMI GGLRS  V+NA++ISYTPL +NPLSPTFYYI
Sbjct: 245 LGRRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNATKISYTPLLINPLSPTFYYI 304

Query: 301 GVKSITVDGVRLPINPAVWSIDELGNGGTVVDSGTTLTFLAEPAYDQVLTAFKRRVKLPK 360
            VKSITVDGV+LPINP VW+I+E GNGGTVVDSGTTLT+LAE AY +VL A ++RVKLP 
Sbjct: 305 AVKSITVDGVKLPINPIVWAINEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRVKLPA 364

Query: 361 PAELTPGFDLCVNASGES-RVSLPRMSFRLAGGSVFSPPPRNYFLETEERVMCLAIRPVN 420
            AELTPGFDLCVN S ES R SLPR+ F+L  G+VF+PP RNYFL+TEE VMCLAIR V 
Sbjct: 365 AAELTPGFDLCVNVSNESQRPSLPRVRFQLGNGAVFAPPARNYFLDTEEGVMCLAIRAVE 424

Query: 421 SGNGFSIIGNLMQQGFLLEFDKDASRLGFSRRGCGLP 454
            GNGFS+IGNLMQQGFLLEFDK+ASRLGFSRRGCGLP
Sbjct: 425 GGNGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP 459

BLAST of Sgr017073 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 3.6e-66
Identity = 161/426 (37.79%), Postives = 229/426 (53.76%), Query Frame = 0

Query: 38  HKNPFSSPSEALSFDTHR---LSVLFSALHGRNTLKSP--------VISGASTGSGQYFV 97
           +K P    S  L  D+ R   ++ L + + GRN   +P        V+SG S GSG+YF 
Sbjct: 85  NKTPDELFSSRLQRDSRRVKSIATLAAQIPGRNVTHAPRPGGFSSSVVSGLSQGSGEYFT 144

Query: 98  DLRLGTPPQSLLLVADTGSDLVWVKCSACRNCSHHSPGSAFLPRHSSTFSPYHCYDSSCH 157
            L +GTP + + +V DTGSD+VW++C+ CR C   S    F PR S T++   C    C 
Sbjct: 145 RLGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQS-DPIFDPRKSKTYATIPCSSPHCR 204

Query: 158 LVTHPDHRVCNHTRLHTPCPYQYSYADGSLSNGFFSKEATTLKTVSGREARIRNLSFGCG 217
            +   D   CN TR  T C YQ SY DGS + G FS E     T++ R  R++ ++ GCG
Sbjct: 205 RL---DSAGCN-TRRKT-CLYQVSYGDGSFTVGDFSTE-----TLTFRRNRVKGVALGCG 264

Query: 218 FRISGPSVSGLSFNGARGVMGLGRGPISFSSQLGRRFGNKFSYCLMDYTFSPPPTSYLMI 277
               G       F GA G++GLG+G +SF  Q G RF  KFSYCL+D + S  P+S +  
Sbjct: 265 HDNEG------LFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSKPSSVV-- 324

Query: 278 GGLRSPVVSNASRISYTPLQMNPLSPTFYYIGVKSITVDGVRLP-INPAVWSIDELGNGG 337
               +  VS  +R  +TPL  NP   TFYY+G+  I+V G R+P +  +++ +D++GNGG
Sbjct: 325 --FGNAAVSRIAR--FTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGG 384

Query: 338 TVVDSGTTLTFLAEPAYDQVLTAFKRRVKLPKPAELTPGFDLCVNASGESRVSLPRMSFR 397
            ++DSGT++T L  PAY  +  AF+   K  K A     FD C + S  + V +P +   
Sbjct: 385 VIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEVKVPTVVLH 444

Query: 398 LAGGSVFSPPPRNYFLETEER-VMCLAIRPVNSGNGFSIIGNLMQQGFLLEFDKDASRLG 451
             G  V S P  NY +  +     C A     +  G SIIGN+ QQGF + +D  +SR+G
Sbjct: 445 FRGADV-SLPATNYLIPVDTNGKFCFAF--AGTMGGLSIIGNIQQQGFRVVYDLASSRVG 484

BLAST of Sgr017073 vs. ExPASy Swiss-Prot
Match: Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 218.4 bits (555), Expect = 1.7e-55
Identity = 147/440 (33.41%), Postives = 217/440 (49.32%), Query Frame = 0

Query: 34  LPLLHKNPFSSPS---------EALSFDTHRLSVLFSALHGR-----------NTLKSPV 93
           L LLH++ F S +           +  DT R+S +   + G+           N   S +
Sbjct: 61  LRLLHRDRFPSVTYRNHHHRLHARMRRDTDRVSAILRRISGKVIPSSDSRYEVNDFGSDI 120

Query: 94  ISGASTGSGQYFVDLRLGTPPQSLLLVADTGSDLVWVKCSACRNCSHHSPGSAFLPRHSS 153
           +SG   GSG+YFV + +G+PP+   +V D+GSD+VWV+C  C+ C   S    F P  S 
Sbjct: 121 VSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKLCYKQS-DPVFDPAKSG 180

Query: 154 TFSPYHCYDSSCHLVTHPDHRVCNHTRLHTPCPYQYSYADGSLSNGFFSKEATTL-KTVS 213
           +++   C  S C        R+ N       C Y+  Y DGS + G  + E  T  KTV 
Sbjct: 181 SYTGVSCGSSVC-------DRIENSGCHSGGCRYEVMYGDGSYTKGTLALETLTFAKTV- 240

Query: 214 GREARIRNLSFGCGFRISGPSVSGLSFNGARGVMGLGRGPISFSSQLGRRFGNKFSYCLM 273
                +RN++ GCG R  G       F GA G++G+G G +SF  QL  + G  F YCL+
Sbjct: 241 -----VRNVAMGCGHRNRG------MFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLV 300

Query: 274 DYTFSPPPTSYLMIGGLRSPVVSNASRISYTPLQMNPLSPTFYYIGVKSITVDGVRLPIN 333
             +     T  L+ G    PV +     S+ PL  NP +P+FYY+G+K + V GVR+P+ 
Sbjct: 301 --SRGTDSTGSLVFGREALPVGA-----SWVPLVRNPRAPSFYYVGLKGLGVGGVRIPLP 360

Query: 334 PAVWSIDELGNGGTVVDSGTTLTFLAEPAYDQVLTAFK-RRVKLPKPAELTPGFDLCVNA 393
             V+ + E G+GG V+D+GT +T L   AY      FK +   LP+ + ++  FD C + 
Sbjct: 361 DGVFDLTETGDGGVVMDTGTAVTRLPTAAYVAFRDGFKSQTANLPRASGVSI-FDTCYDL 420

Query: 394 SGESRVSLPRMSFRLAGGSVFSPPPRNYFLETEER-VMCLAIRPVNSGNGFSIIGNLMQQ 451
           SG   V +P +SF    G V + P RN+ +  ++    C A     S  G SIIGN+ Q+
Sbjct: 421 SGFVSVRVPTVSFYFTEGPVLTLPARNFLMPVDDSGTYCFAF--AASPTGLSIIGNIQQE 470

BLAST of Sgr017073 vs. ExPASy Swiss-Prot
Match: Q9LTW4 (Aspartic proteinase NANA, chloroplast OS=Arabidopsis thaliana OX=3702 GN=NANA PE=1 SV=1)

HSP 1 Score: 218.4 bits (555), Expect = 1.7e-55
Identity = 160/462 (34.63%), Postives = 231/462 (50.00%), Query Frame = 0

Query: 3   SLSPCFPLLLLLFFFTDFCSIPKTAATEYLKLPLLHKN-----PFSSPSEALSFDTHRLS 62
           +L  C    LLL    D  S+  T+    ++L L H++     P S   + +  D  R S
Sbjct: 26  TLLSCLITTLLLITVAD--SMKDTS----VRLKLAHRDTLLPKPLSRIEDVIGADQKRHS 85

Query: 63  VLFSALHGRNTLKSPVISGASTGSGQYFVDLRLGTPPQSLLLVADTGSDLVWVKCSACRN 122
           ++    +    +K  + SG   G+ QYF ++R+GTP +   +V DTGS+L WV      N
Sbjct: 86  LISRKRNSTVGVKMDLGSGIDYGTAQYFTEIRVGTPAKKFRVVVDTGSELTWV------N 145

Query: 123 CSHHSPGS----AFLPRHSSTFSPYHCYDSSCHLVTHPDHRVCNHTRLHTPCPYQYSYAD 182
           C + + G      F    S +F    C   +C +       +       TPC Y Y YAD
Sbjct: 146 CRYRARGKDNRRVFRADESKSFKTVGCLTQTCKVDLMNLFSLTTCPTPSTPCSYDYRYAD 205

Query: 183 GSLSNGFFSKEATTLKTVSGREARIRNLSFGCGFRISGPSVSGLSFNGARGVMGLGRGPI 242
           GS + G F+KE  T+   +GR AR+     GC       S +G SF GA GV+GL     
Sbjct: 206 GSAAQGVFAKETITVGLTNGRMARLPGHLIGC-----SSSFTGQSFQGADGVLGLAFSDF 265

Query: 243 SFSSQLGRRFGNKFSYCLMDYTFSPPPTSYLMIGGLRSPVVSNASRISYTPLQMNPLSPT 302
           SF+S     +G KFSYCL+D+  +   ++YL+ G  RS     A R + TPL +  + P 
Sbjct: 266 SFTSTATSLYGAKFSYCLVDHLSNKNVSNYLIFGSSRS--TKTAFRRT-TPLDLTRI-PP 325

Query: 303 FYYIGVKSITVDGVRLPINPAVWSIDELGNGGTVVDSGTTLTFLAEPAYDQVLTAFKR-- 362
           FY I V  I++    L I   VW  D    GGT++DSGT+LT LA+ AY QV+T   R  
Sbjct: 326 FYAINVIGISLGYDMLDIPSQVW--DATSGGGTILDSGTSLTLLADAAYKQVVTGLARYL 385

Query: 363 -RVKLPKPAELTPGFDLCVN-ASGESRVSLPRMSFRLAGGSVFSPPPRNYFLETEERVMC 422
             +K  KP E  P  + C +  SG +   LP+++F L GG+ F P  ++Y ++    V C
Sbjct: 386 VELKRVKP-EGVP-IEYCFSFTSGFNVSKLPQLTFHLKGGARFEPHRKSYLVDAAPGVKC 445

Query: 423 LAIRPVNSGN-GFSIIGNLMQQGFLLEFDKDASRLGFSRRGC 451
           L    V++G    ++IGN+MQQ +L EFD  AS L F+   C
Sbjct: 446 LGF--VSAGTPATNVIGNIMQQNYLWEFDLMASTLSFAPSAC 460

BLAST of Sgr017073 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 218.0 bits (554), Expect = 2.2e-55
Identity = 139/412 (33.74%), Postives = 205/412 (49.76%), Query Frame = 0

Query: 42  FSSPSEALSFDTHRLSVLFSALHGRNTLKSPVISGASTGSGQYFVDLRLGTPPQSLLLVA 101
           F     A+   + RL  L + L+G + +++ V +    G G+Y ++L +GTP Q    + 
Sbjct: 57  FQLLERAIERGSRRLQRLEAMLNGPSGVETSVYA----GDGEYLMNLSIGTPAQPFSAIM 116

Query: 102 DTGSDLVWVKCSACRNCSHHSPGSAFLPRHSSTFSPYHCYDSSCHLVTHPDHRVCNHTRL 161
           DTGSDL+W +C  C  C + S    F P+ SS+FS   C    C  ++ P       T  
Sbjct: 117 DTGSDLIWTQCQPCTQCFNQST-PIFNPQGSSSFSTLPCSSQLCQALSSP-------TCS 176

Query: 162 HTPCPYQYSYADGSLSNGFFSKEATTLKTVSGREARIRNLSFGCGFRISGPSVSGLSFNG 221
           +  C Y Y Y DGS + G    E  T  +VS     I N++FGC     G +  G     
Sbjct: 177 NNFCQYTYGYGDGSETQGSMGTETLTFGSVS-----IPNITFGC-----GENNQGFGQGN 236

Query: 222 ARGVMGLGRGPISFSSQLGRRFGNKFSYCLMDYTFSPPPTSYLMIGGLRSPVVSNASRIS 281
             G++G+GRGP+S  SQL      KFSYC+     S P  S L++G L + V + +    
Sbjct: 237 GAGLVGMGRGPLSLPSQLD---VTKFSYCMTPIGSSTP--SNLLLGSLANSVTAGSPN-- 296

Query: 282 YTPLQMNPLSPTFYYIGVKSITVDGVRLPINPAVWSID-ELGNGGTVVDSGTTLTFLAEP 341
            T L  +   PTFYYI +  ++V   RLPI+P+ ++++   G GG ++DSGTTLT+    
Sbjct: 297 -TTLIQSSQIPTFYYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTLTYFVNN 356

Query: 342 AYDQVLTAFKRRVKLPKPAELTPGFDLCVNA-SGESRVSLPRMSFRLAGGSVFSPPPRNY 401
           AY  V   F  ++ LP     + GFDLC    S  S + +P       GG +   P  NY
Sbjct: 357 AYQSVRQEFISQINLPVVNGSSSGFDLCFQTPSDPSNLQIPTFVMHFDGGDL-ELPSENY 416

Query: 402 FLETEERVMCLAIRPVNSGNGFSIIGNLMQQGFLLEFDKDASRLGFSRRGCG 452
           F+     ++CLA+   +S  G SI GN+ QQ  L+ +D   S + F+   CG
Sbjct: 417 FISPSNGLICLAMG--SSSQGMSIFGNIQQQNMLVVYDTGNSVVSFASAQCG 435

BLAST of Sgr017073 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 207.2 bits (526), Expect = 3.8e-52
Identity = 129/388 (33.25%), Postives = 192/388 (49.48%), Query Frame = 0

Query: 69  LKSPVISGASTGSGQYFVDLRLGTPPQSLLLVADTGSDLVWVKCSACRNCSHHSPGSAFL 128
           L +PV+SGAS GSG+YF  + +GTP + + LV DTGSD+ W++C  C +C   S    F 
Sbjct: 147 LTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVLDTGSDVNWIQCEPCADCYQQS-DPVFN 206

Query: 129 PRHSSTFSPYHCYDSSCHLVTHPDHRVCNHTRLHTPCPYQYSYADGSLSNGFFSKEATTL 188
           P  SST+    C    C L+   +   C   +    C YQ SY DGS + G  + +  T 
Sbjct: 207 PTSSSTYKSLTCSAPQCSLL---ETSACRSNK----CLYQVSYGDGSFTVGELATDTVTF 266

Query: 189 KTVSGREARIRNLSFGCGFRISGPSVSGLSFNGARGVMGLGRGPISFSSQLGRRFGNKFS 248
               G   +I N++ GCG    G       F GA G++GLG G +S ++Q+       FS
Sbjct: 267 ----GNSGKINNVALGCGHDNEG------LFTGAAGLLGLGGGVLSITNQMK---ATSFS 326

Query: 249 YCLMDYTFSPPPTSYLMIGGLRSPVVSNASRI----SYTPLQMNPLSPTFYYIGVKSITV 308
           YCL+D              G  S +  N+ ++    +  PL  N    TFYY+G+   +V
Sbjct: 327 YCLVDRD-----------SGKSSSLDFNSVQLGGGDATAPLLRNKKIDTFYYVGLSGFSV 386

Query: 309 DGVRLPINPAVWSIDELGNGGTVVDSGTTLTFLAEPAYDQVLTAF-KRRVKLPKPAELTP 368
            G ++ +  A++ +D  G+GG ++D GT +T L   AY+ +  AF K  V L K +    
Sbjct: 387 GGEKVVLPDAIFDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSIS 446

Query: 369 GFDLCVNASGESRVSLPRMSFRLAGGSVFSPPPRNYFLETEER-VMCLAIRPVNSGNGFS 428
            FD C + S  S V +P ++F   GG     P +NY +  ++    C A  P +S    S
Sbjct: 447 LFDTCYDFSSLSTVKVPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSS--SLS 500

Query: 429 IIGNLMQQGFLLEFDKDASRLGFSRRGC 451
           IIGN+ QQG  + +D   + +G S   C
Sbjct: 507 IIGNVQQQGTRITYDLSKNVIGLSGNKC 500

BLAST of Sgr017073 vs. ExPASy TrEMBL
Match: A0A6J1ELQ4 (aspartyl protease family protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111435697 PE=3 SV=1)

HSP 1 Score: 714.1 bits (1842), Expect = 3.6e-202
Identity = 354/457 (77.46%), Postives = 393/457 (86.00%), Query Frame = 0

Query: 1   MLSLSPCFPLLLLLFFFTDFCSIPKTAATEYLKLPLLHKNPFSSPSEALSFDTHRLSVLF 60
           MLS+SP F L+LLLF   D   +   AA +YLKLPLLHKNPFSSPS+ALS DTHRLS+LF
Sbjct: 5   MLSVSPFFHLILLLFSLADL--LNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSLLF 64

Query: 61  SALHGR--NTLKSPVISGASTGSGQYFVDLRLGTPPQSLLLVADTGSDLVWVKCSACRNC 120
           SAL  R   TLKSP+ISGASTGSGQYFVDLR+GTPPQSLLLVADTGSDLVWVKCSACRNC
Sbjct: 65  SALRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNC 124

Query: 121 SHHSPGSAFLPRHSSTFSPYHCYDSSCHLVTHPDHRVCNHTRLHTPCPYQYSYADGSLSN 180
           SHH P SAFLPRHSS+FSP+HC+D  C L+ H    +CNHTRLH+PC + Y+YADGS S+
Sbjct: 125 SHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGSTSS 184

Query: 181 GFFSKEATTLKTVSGREARIRNLSFGCGFRISGPSVSGLSFNGARGVMGLGRGPISFSSQ 240
           GFFSKE TTLKT++G E R+++LSFGCGFRISGPSVSG  FNGARGVMGLGRGPISFS+Q
Sbjct: 185 GFFSKETTTLKTLTGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQ 244

Query: 241 LGRRFGNKFSYCLMDYTFSPPPTSYLMI-GGLRSPVVSNASRISYTPLQMNPLSPTFYYI 300
           LGRRFGNKFSYCLMDYT SPPPTSYLMI GGLR   V+NA++ISYTPL +NPLSPTFYYI
Sbjct: 245 LGRRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRRLPVTNATKISYTPLLINPLSPTFYYI 304

Query: 301 GVKSITVDGVRLPINPAVWSIDELGNGGTVVDSGTTLTFLAEPAYDQVLTAFKRRVKLPK 360
            VKSITVDGV+LPINP +W+IDE GNGGTVVDSGTTLT+LAE AY +VL A ++RVKLP 
Sbjct: 305 AVKSITVDGVKLPINPTLWAIDEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRVKLPA 364

Query: 361 PAELTPGFDLCVNASGES-RVSLPRMSFRLAGGSVFSPPPRNYFLETEERVMCLAIRPVN 420
            AELTPGFDLCVN S ES R SLPR+ F+L  G+VF PP RNYFLETEE VMCLAIR V 
Sbjct: 365 AAELTPGFDLCVNVSNESQRPSLPRVRFQLGNGAVFPPPARNYFLETEEGVMCLAIRAVE 424

Query: 421 SGNGFSIIGNLMQQGFLLEFDKDASRLGFSRRGCGLP 454
            GNGFS+IGNLMQQGFLLEFDK+ASRLGFSRRGCGLP
Sbjct: 425 GGNGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP 459

BLAST of Sgr017073 vs. ExPASy TrEMBL
Match: A0A6J1HXS2 (aspartyl protease family protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111469001 PE=3 SV=1)

HSP 1 Score: 712.6 bits (1838), Expect = 1.0e-201
Identity = 355/457 (77.68%), Postives = 393/457 (86.00%), Query Frame = 0

Query: 1   MLSLSPCFPLLLLLFFFTDFCSIPKTAATEYLKLPLLHKNPFSSPSEALSFDTHRLSVLF 60
           MLS+S  F L+LLLF   D   +   AA +YLKLPLLHKNPFSSPS+ALS DTHRLS+LF
Sbjct: 5   MLSVSTFFHLILLLFSLADL--LNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSLLF 64

Query: 61  SALHGR--NTLKSPVISGASTGSGQYFVDLRLGTPPQSLLLVADTGSDLVWVKCSACRNC 120
           SAL  R   TLKSP+ISGASTGSGQYFVDLR+GTPPQSLLLVADTGSDLVWVKCSACRNC
Sbjct: 65  SALRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNC 124

Query: 121 SHHSPGSAFLPRHSSTFSPYHCYDSSCHLVTHPDHRVCNHTRLHTPCPYQYSYADGSLSN 180
           SHH P SAFLPRHSS+FSP+HC+D  C L+ H    +CNHTRLH+PC + Y+YADGS S+
Sbjct: 125 SHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGSTSS 184

Query: 181 GFFSKEATTLKTVSGREARIRNLSFGCGFRISGPSVSGLSFNGARGVMGLGRGPISFSSQ 240
           GFFSKE TTLKT+SG E R+++LSFGCGFRISGPSVSG  FNGARGVMGLGRGPISFS+Q
Sbjct: 185 GFFSKETTTLKTLSGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQ 244

Query: 241 LGRRFGNKFSYCLMDYTFSPPPTSYLMI-GGLRSPVVSNASRISYTPLQMNPLSPTFYYI 300
           LGRRFGNKFSYCLMDYT SPPPTSYLMI GGLRS  V+NA++ISYTPL +NPLSPTFYYI
Sbjct: 245 LGRRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNATKISYTPLLINPLSPTFYYI 304

Query: 301 GVKSITVDGVRLPINPAVWSIDELGNGGTVVDSGTTLTFLAEPAYDQVLTAFKRRVKLPK 360
            VKSITVDGV+LPINP VW+IDE GNGGTVVDSGTTLT+LAE AY +VL A ++RVKLP 
Sbjct: 305 AVKSITVDGVKLPINPTVWAIDEQGNGGTVVDSGTTLTYLAEEAYKEVLKAVRQRVKLPA 364

Query: 361 PAELTPGFDLCVNASGES-RVSLPRMSFRLAGGSVFSPPPRNYFLETEERVMCLAIRPVN 420
            AELTPGFDLCVN S ES R SLPR+ FR+  G+VF+PP RNYFLET E VMCLAIR V 
Sbjct: 365 AAELTPGFDLCVNVSKESQRPSLPRVRFRVGNGAVFAPPARNYFLETVEGVMCLAIRAVE 424

Query: 421 SGNGFSIIGNLMQQGFLLEFDKDASRLGFSRRGCGLP 454
            GNGFS+IGNLMQQGFLLEFDK+ASRLGFSRRGCGLP
Sbjct: 425 GGNGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP 459

BLAST of Sgr017073 vs. ExPASy TrEMBL
Match: A0A0A0KNH6 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G174650 PE=3 SV=1)

HSP 1 Score: 707.2 bits (1824), Expect = 4.4e-200
Identity = 348/459 (75.82%), Postives = 391/459 (85.19%), Query Frame = 0

Query: 1   MLSLSPCFPLLLLLFFFTDFCSIPKTAA----TEYLKLPLLHKNPFSSPSEALSFDTHRL 60
           +LS+SP F L+LL  FF      P   A     ++LKLPLLHK PFSSPS++LS DTHRL
Sbjct: 3   VLSISPFFLLILLFSFFLTHLPNPNATAVAAPADFLKLPLLHKPPFSSPSQSLSSDTHRL 62

Query: 61  SVLFSALHGRNTLKSPVISGASTGSGQYFVDLRLGTPPQSLLLVADTGSDLVWVKCSACR 120
           S+LFS      TLKSP+ISGASTGSGQYFVD+RLGTPPQSLLLVADTGSDLVWVKCSACR
Sbjct: 63  SLLFS--RPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACR 122

Query: 121 NCSHHSPGSAFLPRHSSTFSPYHCYDSSCHLVTHPDHRVCNHTRLHTPCPYQYSYADGSL 180
           NCSHH P SAFLPRHSS+FSP+HC+D  C L+ H  H +CNHTRLH+PC + YSYADGSL
Sbjct: 123 NCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPHHLCNHTRLHSPCRFLYSYADGSL 182

Query: 181 SNGFFSKEATTLKTVSGREARIRNLSFGCGFRISGPSVSGLSFNGARGVMGLGRGPISFS 240
           S+GFFSKE TTLK++SG E  ++ LSFGCGFRISGPSVSG  FNGARGVMGLGRG ISFS
Sbjct: 183 SSGFFSKETTTLKSLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFS 242

Query: 241 SQLGRRFGNKFSYCLMDYTFSPPPTSYLMI-GGLRSPVVSNASRISYTPLQMNPLSPTFY 300
           SQLGRRFGNKFSYCLMDYT SPPPTS+LMI GGL S  ++NA++ISYTPLQ+NPLSPTFY
Sbjct: 243 SQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPLTNATKISYTPLQINPLSPTFY 302

Query: 301 YIGVKSITVDGVRLPINPAVWSIDELGNGGTVVDSGTTLTFLAEPAYDQVLTAFKRRVKL 360
           YI + SIT+DGV+LPINPAVW IDE GNGGTVVDSGTTLT+L + AY++VL + +RRVKL
Sbjct: 303 YITIHSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKL 362

Query: 361 PKPAELTPGFDLCVNASGES-RVSLPRMSFRLAGGSVFSPPPRNYFLETEERVMCLAIRP 420
           P  AELTPGFDLCVNASGES R SLPR+ FRL GG+VF+PPPRNYFLETEE VMCLAIR 
Sbjct: 363 PNAAELTPGFDLCVNASGESRRPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRA 422

Query: 421 VNSGNGFSIIGNLMQQGFLLEFDKDASRLGFSRRGCGLP 454
           V SGNGFS+IGNLMQQGFLLEFDK+ SRLGF+RRGCGLP
Sbjct: 423 VESGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 459

BLAST of Sgr017073 vs. ExPASy TrEMBL
Match: A0A1S3CSZ8 (aspartyl protease family protein 2 OS=Cucumis melo OX=3656 GN=LOC103504612 PE=3 SV=1)

HSP 1 Score: 706.1 bits (1821), Expect = 9.7e-200
Identity = 350/459 (76.25%), Postives = 389/459 (84.75%), Query Frame = 0

Query: 1   MLSLSPCFPLLLLLFFFTDFCSIPK----TAATEYLKLPLLHKNPFSSPSEALSFDTHRL 60
           MLS+SP F L+ L FFF    S P      AA ++LKLPLLHK PFSSPS++LS DTHRL
Sbjct: 3   MLSISPFFLLIPLFFFFLTHLSNPNATAVAAAADFLKLPLLHKPPFSSPSQSLSSDTHRL 62

Query: 61  SVLFSALHGRNTLKSPVISGASTGSGQYFVDLRLGTPPQSLLLVADTGSDLVWVKCSACR 120
           S+LFS      TLKSP+ISGASTGSGQYFVD+RLGTPPQSLLLVADTGSDLVWVKCSACR
Sbjct: 63  SLLFS--RPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACR 122

Query: 121 NCSHHSPGSAFLPRHSSTFSPYHCYDSSCHLVTHPDHRVCNHTRLHTPCPYQYSYADGSL 180
           NCSHH P SAF PRHSS+FSP+HC+D  C L+ H     CNHT LH+PC + YSYADGSL
Sbjct: 123 NCSHHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHAPPHHCNHTLLHSPCRFLYSYADGSL 182

Query: 181 SNGFFSKEATTLKTVSGREARIRNLSFGCGFRISGPSVSGLSFNGARGVMGLGRGPISFS 240
           S+GFFSKE TTLKT+SG E  ++ LSFGCGFRISGPSVSG  FNGARGVMGLGRG ISFS
Sbjct: 183 SSGFFSKETTTLKTLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFS 242

Query: 241 SQLGRRFGNKFSYCLMDYTFSPPPTSYLMI-GGLRSPVVSNASRISYTPLQMNPLSPTFY 300
           SQLGRRFGNKFSYCLMDYT SPPPTS+LMI GGL S  V+NA++ISYTPLQ+NPLSPTFY
Sbjct: 243 SQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPVNNATKISYTPLQINPLSPTFY 302

Query: 301 YIGVKSITVDGVRLPINPAVWSIDELGNGGTVVDSGTTLTFLAEPAYDQVLTAFKRRVKL 360
           YI + SIT+DGV+LPINPAVW IDE GNGGTVVDSGTTLT+L + AY++VL + +RRVKL
Sbjct: 303 YITINSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKL 362

Query: 361 PKPAELTPGFDLCVNASGES-RVSLPRMSFRLAGGSVFSPPPRNYFLETEERVMCLAIRP 420
           P  AELTPGFDLCVNASGES R SLPR+ FRL GG+VF+PPPRNYFLETEE VMCLAIR 
Sbjct: 363 PNAAELTPGFDLCVNASGESRRPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRA 422

Query: 421 VNSGNGFSIIGNLMQQGFLLEFDKDASRLGFSRRGCGLP 454
           V SGNGFS+IGNLMQQGFLLEFDK+ SRLGF+RRGCGLP
Sbjct: 423 VESGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 459

BLAST of Sgr017073 vs. ExPASy TrEMBL
Match: A0A6J1EPZ3 (aspartyl protease family protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111436585 PE=3 SV=1)

HSP 1 Score: 688.3 bits (1775), Expect = 2.1e-194
Identity = 337/454 (74.23%), Postives = 379/454 (83.48%), Query Frame = 0

Query: 6   PCFP--LLLLLFFFTDFCSIPKTAATEYLKLPLLHKNPFSSPSEALSFDTHRLSVLFSAL 65
           PC P  LLLLL   +    +     ++YLK PLLH NPFSSPS+ALS DTHRLS+LFSA 
Sbjct: 2   PCVPQFLLLLLLLLSSLADLSNAIPSQYLKFPLLHTNPFSSPSQALSSDTHRLSLLFSAH 61

Query: 66  HGRNTLKSPVISGASTGSGQYFVDLRLGTPPQSLLLVADTGSDLVWVKCSACRNCSHHSP 125
               TLKSP+ISGASTGSGQYFV+L LGTPPQSLLLV DTGSDLVWVKCS CRNCSHH P
Sbjct: 62  RHSPTLKSPLISGASTGSGQYFVNLHLGTPPQSLLLVVDTGSDLVWVKCSPCRNCSHHPP 121

Query: 126 GSAFLPRHSSTFSPYHCYDSSCHLVTHPDHRVCNHTRLHTPCPYQYSYADGSLSNGFFSK 185
            SAF PRHSS+FSP+HC+D  C L+ HP    CNHT LH+PC + YSYAD SLS+GFFSK
Sbjct: 122 SSAFFPRHSSSFSPFHCFDPHCRLLPHPPSHRCNHTHLHSPCSFLYSYADSSLSSGFFSK 181

Query: 186 EATTLKTVSG--REARIRNLSFGCGFRISGPSVSGLSFNGARGVMGLGRGPISFSSQLGR 245
           + TT  T SG   + R+ +LSFGCGFRISGPSVSG  F GARGVMGLGRGPISFSSQLG 
Sbjct: 182 DVTTFNTFSGTHTQTRLNDLSFGCGFRISGPSVSGARFTGARGVMGLGRGPISFSSQLGH 241

Query: 246 RFGNKFSYCLMDYTFSPPPTSYLMI-GGLRSPVVSNASRISYTPLQMNPLSPTFYYIGVK 305
           RFGN FSYCLMDYT SPPPTSYLMI GGLRS  V+NAS+ISYTPLQ+NPLSPTFYYI VK
Sbjct: 242 RFGNTFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNASKISYTPLQINPLSPTFYYIVVK 301

Query: 306 SITVDGVRLPINPAVWSIDELGNGGTVVDSGTTLTFLAEPAYDQVLTAFKRRVKLPKPAE 365
           SITVDGV+LPINP VW+IDE GNGGTVVDSGTTLT+LAE AY++VL A +RRVKLP+  +
Sbjct: 302 SITVDGVKLPINPKVWAIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKAMRRRVKLPRALQ 361

Query: 366 LTPGFDLCVNASGESRV-SLPRMSFRLAGGSVFSPPPRNYFLETEERVMCLAIRPVNSGN 425
           L+PGFDLCVNAS ESR+ SLP++ FR+ GG VF+PP RNYF+ETEE VMCLAIRPV+SGN
Sbjct: 362 LSPGFDLCVNASSESRMRSLPQIRFRVGGGGVFAPPARNYFVETEEGVMCLAIRPVDSGN 421

Query: 426 GFSIIGNLMQQGFLLEFDKDASRLGFSRRGCGLP 454
           GFS+IGNLMQQGFLLEFD++ SR+GFSRRGCGLP
Sbjct: 422 GFSVIGNLMQQGFLLEFDREKSRMGFSRRGCGLP 455

BLAST of Sgr017073 vs. TAIR 10
Match: AT3G25700.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 604.7 bits (1558), Expect = 5.9e-173
Identity = 296/455 (65.05%), Postives = 355/455 (78.02%), Query Frame = 0

Query: 8   FPLLLLLFFFTDFCSIPKT--AA----TEYLKLPLLHKNPFSSPSEALSFDTHRLSVLFS 67
           FPL+  L  F     +P +  AA     +YLKLPLL K+PF SP++AL+ DT RL  L  
Sbjct: 2   FPLIFFLCSFLSLFLLPPSNIAAVSNHNKYLKLPLLRKSPFPSPTQALALDTRRLHFLSL 61

Query: 68  ALHGRNTLKSPVISGASTGSGQYFVDLRLGTPPQSLLLVADTGSDLVWVKCSACRNCSHH 127
                  +KSPV+SGA++GSGQYFVDLR+G PPQSLLL+ADTGSDLVWVKCSACRNCSHH
Sbjct: 62  RRKPIPFVKSPVVSGAASGSGQYFVDLRIGQPPQSLLLIADTGSDLVWVKCSACRNCSHH 121

Query: 128 SPGSAFLPRHSSTFSPYHCYDSSCHLVTHPDHR-VCNHTRLHTPCPYQYSYADGSLSNGF 187
           SP + F PRHSSTFSP HCYD  C LV  PD   +CNHTR+H+ C Y+Y YADGSL++G 
Sbjct: 122 SPATVFFPRHSSTFSPAHCYDPVCRLVPKPDRAPICNHTRIHSTCHYEYGYADGSLTSGL 181

Query: 188 FSKEATTLKTVSGREARIRNLSFGCGFRISGPSVSGLSFNGARGVMGLGRGPISFSSQLG 247
           F++E T+LKT SG+EAR+++++FGCGFRISG SVSG SFNGA GVMGLGRGPISF+SQLG
Sbjct: 182 FARETTSLKTSSGKEARLKSVAFGCGFRISGQSVSGTSFNGANGVMGLGRGPISFASQLG 241

Query: 248 RRFGNKFSYCLMDYTFSPPPTSYLMIGGLRSPVVSNASRISYTPLQMNPLSPTFYYIGVK 307
           RRFGNKFSYCLMDYT SPPPTSYL+IG     +    S++ +TPL  NPLSPTFYY+ +K
Sbjct: 242 RRFGNKFSYCLMDYTLSPPPTSYLIIGNGGDGI----SKLFFTPLLTNPLSPTFYYVKLK 301

Query: 308 SITVDGVRLPINPAVWSIDELGNGGTVVDSGTTLTFLAEPAYDQVLTAFKRRVKLPKPAE 367
           S+ V+G +L I+P++W ID+ GNGGTVVDSGTTL FLAEPAY  V+ A +RRVKLP    
Sbjct: 302 SVFVNGAKLRIDPSIWEIDDSGNGGTVVDSGTTLAFLAEPAYRSVIAAVRRRVKLPIADA 361

Query: 368 LTPGFDLCVNASGESRVS--LPRMSFRLAGGSVFSPPPRNYFLETEERVMCLAIRPVNSG 427
           LTPGFDLCVN SG ++    LPR+ F  +GG+VF PPPRNYF+ETEE++ CLAI+ V+  
Sbjct: 362 LTPGFDLCVNVSGVTKPEKILPRLKFEFSGGAVFVPPPRNYFIETEEQIQCLAIQSVDPK 421

Query: 428 NGFSIIGNLMQQGFLLEFDKDASRLGFSRRGCGLP 454
            GFS+IGNLMQQGFL EFD+D SRLGFSRRGC LP
Sbjct: 422 VGFSVIGNLMQQGFLFEFDRDRSRLGFSRRGCALP 452

BLAST of Sgr017073 vs. TAIR 10
Match: AT3G25700.2 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 415.6 bits (1067), Expect = 5.1e-116
Identity = 226/455 (49.67%), Postives = 270/455 (59.34%), Query Frame = 0

Query: 8   FPLLLLLFFFTDFCSIPKT--AA----TEYLKLPLLHKNPFSSPSEALSFDTHRLSVLFS 67
           FPL+  L  F     +P +  AA     +YLKLPLL K+PF SP++AL+ DT RL  L  
Sbjct: 2   FPLIFFLCSFLSLFLLPPSNIAAVSNHNKYLKLPLLRKSPFPSPTQALALDTRRLHFLSL 61

Query: 68  ALHGRNTLKSPVISGASTGSGQYFVDLRLGTPPQSLLLVADTGSDLVWVKCSACRNCSHH 127
                  +KSPV+SGA++GSGQYFVDLR+G PPQSLLL+ADTGSDLVWVKCSACRNCSHH
Sbjct: 62  RRKPIPFVKSPVVSGAASGSGQYFVDLRIGQPPQSLLLIADTGSDLVWVKCSACRNCSHH 121

Query: 128 SPGSAFLPRHSSTFSPYHCYDSSCHLVTHPDHR-VCNHTRLHTPCPYQYSYADGSLSNGF 187
           SP + F PRHSSTFSP HCYD  C LV  PD   +CNHTR+H+ C Y+Y YADGSL++G 
Sbjct: 122 SPATVFFPRHSSTFSPAHCYDPVCRLVPKPDRAPICNHTRIHSTCHYEYGYADGSLTSGL 181

Query: 188 FSKEATTLKTVSGREARIRNLSFGCGFRISGPSVSGLSFNGARGVMGLGRGPISFSSQLG 247
           F++E T+LKT SG+EAR+++++FGCGFRISG SVS                         
Sbjct: 182 FARETTSLKTSSGKEARLKSVAFGCGFRISGQSVS------------------------- 241

Query: 248 RRFGNKFSYCLMDYTFSPPPTSYLMIGGLRSPVVSNASRISYTPLQMNPLSPTFYYIGVK 307
                                                                       
Sbjct: 242 ------------------------------------------------------------ 301

Query: 308 SITVDGVRLPINPAVWSIDELGNGGTVVDSGTTLTFLAEPAYDQVLTAFKRRVKLPKPAE 367
                                GNGGTVVDSGTTL FLAEPAY  V+ A +RRVKLP    
Sbjct: 302 ---------------------GNGGTVVDSGTTLAFLAEPAYRSVIAAVRRRVKLPIADA 350

Query: 368 LTPGFDLCVNASGESRVS--LPRMSFRLAGGSVFSPPPRNYFLETEERVMCLAIRPVNSG 427
           LTPGFDLCVN SG ++    LPR+ F  +GG+VF PPPRNYF+ETEE++ CLAI+ V+  
Sbjct: 362 LTPGFDLCVNVSGVTKPEKILPRLKFEFSGGAVFVPPPRNYFIETEEQIQCLAIQSVDPK 350

Query: 428 NGFSIIGNLMQQGFLLEFDKDASRLGFSRRGCGLP 454
            GFS+IGNLMQQGFL EFD+D SRLGFSRRGC LP
Sbjct: 422 VGFSVIGNLMQQGFLFEFDRDRSRLGFSRRGCALP 350

BLAST of Sgr017073 vs. TAIR 10
Match: AT1G01300.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 253.8 bits (647), Expect = 2.5e-67
Identity = 161/426 (37.79%), Postives = 229/426 (53.76%), Query Frame = 0

Query: 38  HKNPFSSPSEALSFDTHR---LSVLFSALHGRNTLKSP--------VISGASTGSGQYFV 97
           +K P    S  L  D+ R   ++ L + + GRN   +P        V+SG S GSG+YF 
Sbjct: 85  NKTPDELFSSRLQRDSRRVKSIATLAAQIPGRNVTHAPRPGGFSSSVVSGLSQGSGEYFT 144

Query: 98  DLRLGTPPQSLLLVADTGSDLVWVKCSACRNCSHHSPGSAFLPRHSSTFSPYHCYDSSCH 157
            L +GTP + + +V DTGSD+VW++C+ CR C   S    F PR S T++   C    C 
Sbjct: 145 RLGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQS-DPIFDPRKSKTYATIPCSSPHCR 204

Query: 158 LVTHPDHRVCNHTRLHTPCPYQYSYADGSLSNGFFSKEATTLKTVSGREARIRNLSFGCG 217
            +   D   CN TR  T C YQ SY DGS + G FS E     T++ R  R++ ++ GCG
Sbjct: 205 RL---DSAGCN-TRRKT-CLYQVSYGDGSFTVGDFSTE-----TLTFRRNRVKGVALGCG 264

Query: 218 FRISGPSVSGLSFNGARGVMGLGRGPISFSSQLGRRFGNKFSYCLMDYTFSPPPTSYLMI 277
               G       F GA G++GLG+G +SF  Q G RF  KFSYCL+D + S  P+S +  
Sbjct: 265 HDNEG------LFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSKPSSVV-- 324

Query: 278 GGLRSPVVSNASRISYTPLQMNPLSPTFYYIGVKSITVDGVRLP-INPAVWSIDELGNGG 337
               +  VS  +R  +TPL  NP   TFYY+G+  I+V G R+P +  +++ +D++GNGG
Sbjct: 325 --FGNAAVSRIAR--FTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGG 384

Query: 338 TVVDSGTTLTFLAEPAYDQVLTAFKRRVKLPKPAELTPGFDLCVNASGESRVSLPRMSFR 397
            ++DSGT++T L  PAY  +  AF+   K  K A     FD C + S  + V +P +   
Sbjct: 385 VIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEVKVPTVVLH 444

Query: 398 LAGGSVFSPPPRNYFLETEER-VMCLAIRPVNSGNGFSIIGNLMQQGFLLEFDKDASRLG 451
             G  V S P  NY +  +     C A     +  G SIIGN+ QQGF + +D  +SR+G
Sbjct: 445 FRGADV-SLPATNYLIPVDTNGKFCFAF--AGTMGGLSIIGNIQQQGFRVVYDLASSRVG 484

BLAST of Sgr017073 vs. TAIR 10
Match: AT2G42980.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 250.4 bits (638), Expect = 2.8e-66
Identity = 152/386 (39.38%), Postives = 214/386 (55.44%), Query Frame = 0

Query: 75  SGASTGSGQYFVDLRLGTPPQSLLLVADTGSDLVWVKCSACRNCSHHSPGSAFLPRHSST 134
           SG + GSG+YF+D+ +GTPP+   L+ DTGSDL W++C  C +C H + G  + P+ S++
Sbjct: 151 SGMTLGSGEYFMDVLVGTPPKHFSLILDTGSDLNWLQCLPCYDCFHQN-GMFYDPKTSAS 210

Query: 135 FSPYHCYDSSCHLVTHPDHRV-CNHTRLHTPCPYQYSYADGSLSNGFFSKEATT--LKTV 194
           F    C D  C L++ PD  V C     +  CPY Y Y D S + G F+ E  T  L T 
Sbjct: 211 FKNITCNDPRCSLISSPDPPVQCESD--NQSCPYFYWYGDRSNTTGDFAVETFTVNLTTT 270

Query: 195 SG--REARIRNLSFGCGFRISGPSVSGLSFNGARGVMGLGRGPISFSSQLGRRFGNKFSY 254
            G   E ++ N+ FGCG    G       F+GA G++GLGRGP+SFSSQL   +G+ FSY
Sbjct: 271 EGGSSEYKVGNMMFGCGHWNRG------LFSGASGLLGLGRGPLSFSSQLQSLYGHSFSY 330

Query: 255 CLMDYTFSPPPTSYLMIGGLRSPVVSNASRISYTPLQMNPLS--PTFYYIGVKSITVDGV 314
           CL+D   +   +S L+ G      + N + +++T       +   TFYYI +KSI V G 
Sbjct: 331 CLVDRNSNTNVSSKLIFG--EDKDLLNHTNLNFTSFVNGKENSVETFYYIQIKSILVGGK 390

Query: 315 RLPINPAVWSIDELGNGGTVVDSGTTLTFLAEPAYDQVLTAFKRRVKLPKPA-ELTPGFD 374
            L I    W+I   G+GGT++DSGTTL++ AEPAY+ +   F  ++K   P     P  D
Sbjct: 391 ALDIPEETWNISSDGDGGTIIDSGTTLSYFAEPAYEIIKNKFAEKMKENYPIFRDFPVLD 450

Query: 375 LCVNASG--ESRVSLPRMSFRLAGGSVFSPPPRNYFLETEERVMCLAIRPVNSGNGFSII 434
            C N SG  E+ + LP +      G+V++ P  N F+   E ++CLAI        FSII
Sbjct: 451 PCFNVSGIEENNIHLPELGIAFVDGTVWNFPAENSFIWLSEDLVCLAILGTPKST-FSII 510

Query: 435 GNLMQQGFLLEFDKDASRLGFSRRGC 451
           GN  QQ F + +D   SRLGF+   C
Sbjct: 511 GNYQQQNFHILYDTKRSRLGFTPTKC 524

BLAST of Sgr017073 vs. TAIR 10
Match: AT3G61820.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 243.8 bits (621), Expect = 2.6e-64
Identity = 166/451 (36.81%), Postives = 228/451 (50.55%), Query Frame = 0

Query: 28  ATEYLKLPLLHKNPFSSPSEALSFDTHRLSVLFSALH------------GRNTLK----- 87
           +T  L + L H +  SS S+A   D   L +   +L             GRN  K     
Sbjct: 57  STTSLSVHLSHVDALSSFSDASPADLFNLRLQRDSLRVKSITSLAAVSTGRNATKRTPRT 116

Query: 88  -----SPVISGASTGSGQYFVDLRLGTPPQSLLLVADTGSDLVWVKCSACRNCSHHSPGS 147
                  VISG S GSG+YF+ L +GTP  ++ +V DTGSD+VW++CS C+ C ++   +
Sbjct: 117 AGGFSGAVISGLSQGSGEYFMRLGVGTPATNVYMVLDTGSDVVWLQCSPCKAC-YNQTDA 176

Query: 148 AFLPRHSSTFSPYHCYDSSCHLVTHPDHRVCNHTRLHTPCPYQYSYADGSLSNGFFSKEA 207
            F P+ S TF+   C    C  +      V   TR    C YQ SY DGS + G FS E 
Sbjct: 177 IFDPKKSKTFATVPCGSRLCRRLDDSSECV---TRRSKTCLYQVSYGDGSFTEGDFSTE- 236

Query: 208 TTLKTVSGREARIRNLSFGCGFRISGPSVSGLSFNGARGVMGLGRGPISFSSQLGRRFGN 267
               T++   AR+ ++  GCG    G       F GA G++GLGRG +SF SQ   R+  
Sbjct: 237 ----TLTFHGARVDHVPLGCGHDNEG------LFVGAAGLLGLGRGGLSFPSQTKNRYNG 296

Query: 268 KFSYCLMDYT---FSPPPTSYLMIGGLRSPVVSNASRISYTPLQMNPLSPTFYYIGVKSI 327
           KFSYCL+D T    S  P S ++ G    P  S      +TPL  NP   TFYY+ +  I
Sbjct: 297 KFSYCLVDRTSSGSSSKPPSTIVFGNAAVPKTS-----VFTPLLTNPKLDTFYYLQLLGI 356

Query: 328 TVDGVRLP-INPAVWSIDELGNGGTVVDSGTTLTFLAEPAYDQVLTAFKRRVKLPKPAEL 387
           +V G R+P ++ + + +D  GNGG ++DSGT++T L +PAY  +  AF+      K A  
Sbjct: 357 SVGGSRVPGVSESQFKLDATGNGGVIIDSGTSVTRLTQPAYVALRDAFRLGATKLKRAPS 416

Query: 388 TPGFDLCVNASGESRVSLPRMSFRLAGGSVFSPPPRNYFL--ETEERVMCLAIRPVNSGN 447
              FD C + SG + V +P + F   GG V S P  NY +   TE R  C A     +  
Sbjct: 417 YSLFDTCFDLSGMTTVKVPTVVFHFGGGEV-SLPASNYLIPVNTEGR-FCFAF--AGTMG 476

Query: 448 GFSIIGNLMQQGFLLEFDKDASRLGFSRRGC 451
             SIIGN+ QQGF + +D   SR+GF  R C
Sbjct: 477 SLSIIGNIQQQGFRVAYDLVGSRVGFLSRAC 483