Homology
BLAST of Sgr016774 vs. NCBI nr
Match:
XP_022141775.1 (TMV resistance protein N-like [Momordica charantia])
HSP 1 Score: 2598.5 bits (6734), Expect = 0.0e+00
Identity = 1303/1582 (82.36%), Postives = 1417/1582 (89.57%), Query Frame = 0
Query: 1 MEVPSSTPPNRPRMTYDVFISFRGEDTRNNFVGFLHEALRQLGIMTFLDDKKLLIGDNLS 60
MEV S+ PPNRPRMTYDVFISFRGEDTRNNFVGFL++ALRQLGIMTF+DDKKLLIGD+LS
Sbjct: 29 MEVSSTAPPNRPRMTYDVFISFRGEDTRNNFVGFLYQALRQLGIMTFMDDKKLLIGDDLS 88
Query: 61 ENLVRAIEESRSSIVVLSKDYASSKWCLKELAKIIDCMAETTHRVLPVFYHVDPSNVRHQ 120
E V+AIEESRSSIVVLSKDYASSKWCLKELAKI+DCM+ETTHRVLPVFYHVDPS+VRHQ
Sbjct: 89 EKFVKAIEESRSSIVVLSKDYASSKWCLKELAKIMDCMSETTHRVLPVFYHVDPSHVRHQ 148
Query: 121 SGSFKKSFDEHETIAHKFIGEQKEKYLEEVQNWRDSMTKVGLLSGEVVTEHSPEVDIVKK 180
SG+FKKSFD HE A K IG Q+E+YL+EVQNW+DSMTK+G L+GEVVT+HS EVD+VKK
Sbjct: 149 SGNFKKSFDGHEEKAPKLIGVQREEYLKEVQNWKDSMTKIGNLAGEVVTQHSSEVDVVKK 208
Query: 181 ITNQIFERWRPKLVTSDKNLVGMTYRLLKMNVQLGLGLDDVRFLGIVGMGGIGKTTIAKV 240
ITNQIFERWRPK+ TSDKNLVGMT +LL+MNV+LGLGLDDVRF+GIVGMGGIGKTTIAKV
Sbjct: 209 ITNQIFERWRPKIATSDKNLVGMTRQLLEMNVKLGLGLDDVRFVGIVGMGGIGKTTIAKV 268
Query: 241 VYDCITSKFEGSCFLRVSRGSSKQIELVKLQEQLLSRLFLKENVQIWDEDYGAEMIENQL 300
VYDCITSKFEGSCFLRV GSSKQ LV LQEQLLSRLFLKENV+IWDEDYGAEMIENQL
Sbjct: 269 VYDCITSKFEGSCFLRVFGGSSKQCNLVSLQEQLLSRLFLKENVRIWDEDYGAEMIENQL 328
Query: 301 RSRKVLLVLDGVEEKRQVEMLAGSPDWFGPGSRVIITTRNKEVL-QPYEILEYKVDLLDS 360
SRKVLLVLD VEEK Q+EMLA SPDWFGPGSR+IITTRNKE+L QPYEILEYK+ LLD
Sbjct: 329 CSRKVLLVLDEVEEKEQLEMLARSPDWFGPGSRIIITTRNKEILHQPYEILEYKMKLLDD 388
Query: 361 DSALQLFCNRAFGTDYVDSNFMNLSKEIVEKLGRLPLALKVIGSYLNNKNGVIWNETLNR 420
DSALQLFCNRAFG DY+DS+FMNLSK +VEKLGRLPLALKVIGSYLN K+ +IW ETLNR
Sbjct: 389 DSALQLFCNRAFGIDYIDSSFMNLSKGMVEKLGRLPLALKVIGSYLNKKDVLIWKETLNR 448
Query: 421 LIKVDEKYLFDVLKISYDGLGAESQKVFLDLACFFNGRRIDKVIKILESFGYNPHSELHL 480
LIKVDEK+LF+VL ISYDGLGAESQKVFLDLACFFNGRR DKVIKILESFGYNPHSEL+L
Sbjct: 449 LIKVDEKHLFEVLNISYDGLGAESQKVFLDLACFFNGRRTDKVIKILESFGYNPHSELNL 508
Query: 481 FMERCLIEVSHSKILMHDLILALGQEIVRKESLTQLGKQSRIWLQEDVIRAFTMKHGLKY 540
F E+CLIEVSHSKILMHDLI+ALGQEIVRKESLTQLGKQSRIWLQEDV+RAFTMKHGLKY
Sbjct: 509 FTEKCLIEVSHSKILMHDLIVALGQEIVRKESLTQLGKQSRIWLQEDVVRAFTMKHGLKY 568
Query: 541 TEGIVLNLEKKQKELTLEAKLFADMTCLRILEISNVQLSGNIKSLSNLLGLLNWPGYPSK 600
TEGIVLNLEKKQKELTLEAKLFADMTCLRILEISNVQL GNIKSLSNLL LLNWPGYPSK
Sbjct: 569 TEGIVLNLEKKQKELTLEAKLFADMTCLRILEISNVQLYGNIKSLSNLLALLNWPGYPSK 628
Query: 601 CLPSNFQSGYLLELHLCRSSIVRIWEGKKEFEKLKVINVSDSEYLLETPDFSEVPNLERL 660
CLPSNFQSGYLLELHL SSIVRIW+GKKEFE LKVI+VSDSEYLLETP+FS+VP LERL
Sbjct: 629 CLPSNFQSGYLLELHLRHSSIVRIWDGKKEFENLKVIDVSDSEYLLETPNFSKVPKLERL 688
Query: 661 VLRNCRSLYHIHPSINSLSRLIFLDLNGCSSLKSFPSNLRCKNLERLVLSKSGVTSSLEI 720
VL NCR L HIHPSINSL L FLDL CSSLKSF SNLRCKNLERLVLSKSGVTSS EI
Sbjct: 689 VLCNCRRLNHIHPSINSLHLLKFLDLTECSSLKSFSSNLRCKNLERLVLSKSGVTSSPEI 748
Query: 721 KGNMEHLCELRLDGTPIKQLHSSIERLVGLIVLDLRNCIMLSSLPNEIGNLKSLESLLLG 780
+ NME LCEL LDGTPIKQLH SI RLVGLI LDLR CIMLSSLPNEIGNL+SL+SLLLG
Sbjct: 749 EENMERLCELYLDGTPIKQLHPSIGRLVGLIKLDLRKCIMLSSLPNEIGNLESLQSLLLG 808
Query: 781 GCVNLDQIPPSLGNAQCLAYLEISETSICRLPPTIHCLKNLKGLNCDGLSRSIWPSLLRP 840
GC+NLDQ+PPSLGN Q L YLEI ETSIC LPPTIH LKNLKGL DGLS IW SLL P
Sbjct: 809 GCINLDQMPPSLGNVQRLQYLEICETSICHLPPTIHSLKNLKGLKFDGLSGRIWHSLLPP 868
Query: 841 CDILGSDLGISGSKFGLESLEYLTLKGCNLVNEDIPEDLHCFSSLKTLDLSGNDFVRLPE 900
+IL SDLGISGSKFG+ESLEYL+L+GCNLV EDIPEDLH FSSLKTLDLSGN+FVRLPE
Sbjct: 869 SNILESDLGISGSKFGMESLEYLSLRGCNLVEEDIPEDLHYFSSLKTLDLSGNNFVRLPE 928
Query: 901 SVNHLKNLRELRLHDCFKLQHLPQLPTNLVPIVSYTESGTPVRSFPIPYQHGKHFILEFI 960
S+NHLKNLRELRLHDCFKLQHLP+L TNLVPI+S TESG VR+ PIPY H KHFILEFI
Sbjct: 929 SINHLKNLRELRLHDCFKLQHLPELRTNLVPIISCTESGA-VRNLPIPYHHDKHFILEFI 988
Query: 961 PKTNLKLDPKAFEESILKGVLFSSIDDKAEVLECFDEIREGNLIEIEFYQIKSDANNTYG 1020
PKTNLKLDPKAFEESI +G LFSS DD AEVLECFDEIREGN+IEIE YQIK+DANN YG
Sbjct: 989 PKTNLKLDPKAFEESISEGALFSSTDDNAEVLECFDEIREGNVIEIERYQIKNDANNIYG 1048
Query: 1021 VVLSACIQCSPNTTPEEVVTFYSKFFIELEMEENPRIGILPVTSKLTSRTRFWMLFIPFH 1080
VVLSACIQCS NT EV++FY KF IEL MEE+PR GILP+TSKLTSRTRFWMLFIP +
Sbjct: 1049 VVLSACIQCSQNTA-HEVISFYCKFLIELGMEEHPRTGILPLTSKLTSRTRFWMLFIPLN 1108
Query: 1081 MLPKCRLRFKIVVKGSFPSVNVQKCGASILSHQNAPKFLANMFNMMYRTCNQNKFCTTIR 1140
+LPK RLRFKI+ KGS ++N+QKCGAS+LSHQNAP+ LANMFN +YRT NQ++FCTTIR
Sbjct: 1109 LLPKSRLRFKILAKGSPNTINIQKCGASVLSHQNAPELLANMFNKVYRTRNQDEFCTTIR 1168
Query: 1141 ECNHGSECSCDEPETVDQYCTTQYVENESDSFSLLKRNLKSILQRTYEEENHRSLVYFFP 1200
ECNHGS+CSCDEPET+DQYC+TQYVENES FSLLKR+LKSIL+RTYEEE SL YFFP
Sbjct: 1169 ECNHGSKCSCDEPETIDQYCSTQYVENESGPFSLLKRSLKSILRRTYEEEKDHSLYYFFP 1228
Query: 1201 QGQVSSWFRIQQPKDTVAVKLPLNLFREKKWMGLAVFAIFSIPGNAEFVHNHIFTCQISS 1260
+G SWFRIQQPKDTVAVKLPL+LF+EKKWMGLAVFAIFS+PGN+E+VHNH F QI+S
Sbjct: 1229 RGPALSWFRIQQPKDTVAVKLPLDLFKEKKWMGLAVFAIFSVPGNSEYVHNHTFNFQITS 1288
Query: 1261 DEDGRHLMKGGSYSSHSFLIPLSLSQFSPQILFVTFKPRHVFPYVQDGSNHFYVSFISKA 1320
+EDGRHL+KGGSYSS SFLIPLSL Q SPQILFVTF+PRHVFPYVQ+G NHFYVSFIS
Sbjct: 1289 NEDGRHLIKGGSYSSGSFLIPLSLLQSSPQILFVTFEPRHVFPYVQEGLNHFYVSFISIP 1348
Query: 1321 TRLRVESCGARLVYQHNVEGLIHTIMDCVLKSGQELFQEYSGKILSDALTTYERLIWIRV 1380
TRL+VESCGARLVYQHNVEGLI+TIMDCV KS +EL+Q++S +I+ D LTTY R I I+V
Sbjct: 1349 TRLQVESCGARLVYQHNVEGLINTIMDCVPKSREELYQDHSQEIMRDFLTTYHRPIRIQV 1408
Query: 1381 PADEPTTSTSSDLSGHHSTNKLLHENFQRPLFKESTQDRYK-SKFDAIIHGKEIPKFFTS 1440
PAD+ TTSTSSDLSG E+FQ LF ESTQD+Y SK +AIIHGKEIP FFTS
Sbjct: 1409 PADQLTTSTSSDLSGR-------RESFQHSLFMESTQDQYNISKLEAIIHGKEIPDFFTS 1468
Query: 1441 QNMGNEGTVRIPERLYFAKCEVGVLVCAVVAINKQREKLERISELQSCVKVLDLKCEFGV 1500
Q+MGN+G VR PERLYFAKCEVGVLVCAVV IN Q EKLE VKVLDLKCEFGV
Sbjct: 1469 QSMGNKGAVRTPERLYFAKCEVGVLVCAVVVINNQTEKLEH-------VKVLDLKCEFGV 1528
Query: 1501 DSHEVEPEHHLFLPAEALSSQYEPSFIWLSCIPLAEFNVPWMCCDYLKSKVSSNHEELFH 1560
DSHE+EPEHHLF+PAE L S +E SFIWLSCIPL EFN+PWMCCDYL+ KVS+NHEELFH
Sbjct: 1529 DSHEIEPEHHLFVPAEKLLSSFESSFIWLSCIPLTEFNIPWMCCDYLRCKVSTNHEELFH 1588
Query: 1561 VQQCGLHLLYVSERKLVDNLLM 1581
VQQCGLHLLY SERK+VDNLL+
Sbjct: 1589 VQQCGLHLLYASERKMVDNLLI 1594
BLAST of Sgr016774 vs. NCBI nr
Match:
XP_022141862.1 (TMV resistance protein N-like [Momordica charantia])
HSP 1 Score: 2206.8 bits (5717), Expect = 0.0e+00
Identity = 1129/1589 (71.05%), Postives = 1294/1589 (81.43%), Query Frame = 0
Query: 1 MEVPSSTPPNRPRMTYDVFISFRGEDTRNNFVGFLHEALRQLGIMTFLDDKKLLIGDNLS 60
MEVPS+ PPNRPRMTYDVFISFRGEDTRNNFVGFL+EAL QLGIMTF+DD+KLLIGD+LS
Sbjct: 44 MEVPSTAPPNRPRMTYDVFISFRGEDTRNNFVGFLYEALHQLGIMTFMDDRKLLIGDDLS 103
Query: 61 ENLVRAIEESRSSIVVLSKDYASSKWCLKELAKIIDCMAETTHRVLPVFYHVDPSNVRHQ 120
ENL++AIEESRSSIVVLSKDYASSKWCLKEL KI+DCM+ETTH+VLPVFYHVDPS+VRHQ
Sbjct: 104 ENLMKAIEESRSSIVVLSKDYASSKWCLKELVKIMDCMSETTHQVLPVFYHVDPSHVRHQ 163
Query: 121 SGSFKKSFDEHETIAHKFIGEQKEKYLEEVQNWRDSMTKVGLLSGEVVTEHSPEVDIVKK 180
SG+FKKSFDEHE A K G+QKEKYL EV+NW+DSMTK+G L+GEVVT+HS EV+IVKK
Sbjct: 164 SGNFKKSFDEHEEDAKKITGQQKEKYLVEVKNWKDSMTKIGNLTGEVVTQHSSEVNIVKK 223
Query: 181 ITNQIFERWRPKLVTSDKNLVGMTYRLLKMNVQLGLGLDDVRFLGIVGMGGIGKTTIAKV 240
ITNQIFERWRPK+ TSDKNLVGMTY+LLKMNV+LGLGLDDVRF+GIVGMGGIGKTTIAKV
Sbjct: 224 ITNQIFERWRPKIATSDKNLVGMTYQLLKMNVKLGLGLDDVRFVGIVGMGGIGKTTIAKV 283
Query: 241 VYDCITSKFEGSCFLRVSRGSSKQIELVKLQEQLLSRLFLKENVQIWDEDYGAEMIENQL 300
VYDCI S FEGSCFLRV GSSKQ L+ LQEQLLSRLFLKENV++WDEDYGAEMI+NQL
Sbjct: 284 VYDCIKSNFEGSCFLRVFGGSSKQNNLLSLQEQLLSRLFLKENVRMWDEDYGAEMIKNQL 343
Query: 301 RSRKVLLVLDGVEEKRQVEMLAGSPDWFGPGSRVIITTRNKEVL-QPYEILEYKVDLLDS 360
SRKVLLVLDGVEEK Q+EMLA SPDWFGPGSR+IITTRNKE+L QPYEI+EYK+ LLD+
Sbjct: 344 CSRKVLLVLDGVEEKEQLEMLARSPDWFGPGSRIIITTRNKEILHQPYEIIEYKLKLLDA 403
Query: 361 DSALQLFCNRAFGTDYVDSNFMNLSKEIVEKLGRLPLALKVIGSYLNNKNGVIWNETLNR 420
DSALQLFCNRAFG DY+DSNFMNLSKEI+EKLGRLPLALKVIGSYLN K VIW ETL R
Sbjct: 404 DSALQLFCNRAFGIDYIDSNFMNLSKEILEKLGRLPLALKVIGSYLNKKEEVIWKETLYR 463
Query: 421 LIKVDEKYLFDVLKISYDGLGAESQKVFLDLACFFNGRRIDKVIKILESFGYNPHSELHL 480
LIKVDEK LF+VLKISYDGLG ESQKVFLDLAC F+GR+ DKVI+ILE FGY+P+SEL L
Sbjct: 464 LIKVDEKNLFEVLKISYDGLGVESQKVFLDLACLFHGRKKDKVIEILECFGYSPYSELQL 523
Query: 481 FMERCLIEVSHSKILMHDLILALGQEIVRKESLTQLGKQSRIWLQEDVIRAFTMKHGLKY 540
FMERCL+EVS +KILMHDL++ALGQEIV +ESLTQ GKQSRIW QEDV R FT+KHGLKY
Sbjct: 524 FMERCLLEVSPTKILMHDLVVALGQEIVHRESLTQPGKQSRIWFQEDVDRVFTVKHGLKY 583
Query: 541 TEGIVLNLEKKQKELTLEAKLFADMTCLRILEISNVQLSGNIKSLSNLLGLLNWPGYPSK 600
TEGIVLNLEKKQKEL LEAK F DMT LRILEISNVQLSGNI LSNLLG +NWPGYPSK
Sbjct: 584 TEGIVLNLEKKQKELILEAKSFTDMTYLRILEISNVQLSGNINFLSNLLGFINWPGYPSK 643
Query: 601 CLPSNFQSGYLLELHLCRSSIVRIWEGKKEFEKLKVINVSDSEYLLETPDFSEVPNLERL 660
CLP NFQS LLELHL SSI +WEGKK+FEKLKVI+VSDS+YLL TPDFS+VPNLERL
Sbjct: 644 CLPPNFQSRCLLELHLPLSSIEILWEGKKDFEKLKVIDVSDSKYLLGTPDFSKVPNLERL 703
Query: 661 VLRNCRSLYHIHPSINSLSRLIFLDLNGCSSLKSFPSNLRCKNLERLVLSKSGVTSSLEI 720
VLRNCR LYHIHPSIN L+RL LDL+ CSS KSF S+LRCK+LE L+LS+SG+TSS +
Sbjct: 704 VLRNCRKLYHIHPSINDLNRLRLLDLSNCSSFKSFSSHLRCKSLEGLILSESGLTSSPKF 763
Query: 721 KGNMEHLCELRLDGTPIKQLHSSIERLVGLIVLDLRNCIMLSSLPNEIGNLKSLESLLLG 780
+ NMEHL L L GTPIKQLHSSI L+GL++LDLRNCIMLSSLP+EIGNLKSL+ LLL
Sbjct: 764 EVNMEHLSRLHLVGTPIKQLHSSIGCLIGLVLLDLRNCIMLSSLPSEIGNLKSLQVLLLE 823
Query: 781 GCVNLDQIPPSLGNAQCLAYLEISETSICRLPPTIHCLKNLKGLNCDGLSRSIWPSLLRP 840
GC +LDQIPPSLGNAQCL YL+I +TSIC+ PPTIH L+NLK LNC+GLSR IW SLL P
Sbjct: 824 GCKSLDQIPPSLGNAQCLEYLDIGKTSICQAPPTIHYLENLKRLNCEGLSRKIWHSLL-P 883
Query: 841 CDILGSDLGISGSKFGLESLEYLTLKGCNLVNEDIPEDLHCFSSLKTLDLSGNDFVRLPE 900
DI S+ ISG FGL+SLE+L LK CNLV+EDIPEDLHCFSSL++LDLSGN FV+ P+
Sbjct: 884 RDIQRSNRQISGLGFGLKSLEHLCLKNCNLVDEDIPEDLHCFSSLESLDLSGNHFVQYPK 943
Query: 901 SVNHLKNLRELRLHDCFKLQHLPQLPTNLVPIVSYTESGTPVRSFPIPYQHGKHFILEFI 960
S+NHL NL++L HDCFKLQ +P+ NL P S ESG P+ +P Q K F LE+
Sbjct: 944 SINHLVNLKQLMFHDCFKLQQIPKPSINLEPKFSSVESGMPIDQ-SLPCQLDKPFRLEYG 1003
Query: 961 PKTNLKLDPKAFEESILKGVLFSSIDDKAEVLECFDEIREGNLIEIEFYQIKSDANNTYG 1020
+ KLDPK +EE IL+ F + DKAE+LECFDEIREGN +E+E+ Q+K+DAN YG
Sbjct: 1004 SEIKSKLDPKTYEELILERATFGTTCDKAEILECFDEIREGNSVEVEYNQVKNDANIIYG 1063
Query: 1021 VVLSACIQCSPNTTPEEVVTFYSKFFIELEMEENPRIGILPVTSKLTSRTRFWMLFIPFH 1080
VVLSA IQCS NT+ EV TFY F IELE + NP I + T LTSR RFW LFIP H
Sbjct: 1064 VVLSASIQCSKNTSHNEVDTFYCNFSIELETDMNPTITVFYGTFALTSRIRFWTLFIPSH 1123
Query: 1081 MLPKCR------LRFKIVVKGSFPSVNVQKCGASILSHQNAPKFLANMFNMMYRTCNQNK 1140
LPK FK +K SV++Q CGASILSHQNA +F+A MF+ MY C Q K
Sbjct: 1124 KLPKSTSMQNSLCSFKATIKYEVSSVHIQMCGASILSHQNASQFMAKMFSRMY--CIQEK 1183
Query: 1141 FCTTIRECNHGSECSCDEPETVDQYCTTQYVENESDSFSLLKRNLKSILQRTYEEENHRS 1200
F T I NH SEC+CDEPETV+ Q+VENE DSFSLL+RNLKSIL+RTYE+ H
Sbjct: 1184 FQTFIVGGNHWSECNCDEPETVEHNYLIQFVENERDSFSLLRRNLKSILRRTYEDTKHIF 1243
Query: 1201 LVYFFPQGQVSSWFRIQQPKDTVAVKLPLNLFREKKWMGLAVFAIFSIPGNAEFVHNHIF 1260
YFFPQ QV SWFRIQQ DTV +KLP N+FREKKWMGLA+FA+FS+ N+EFV +H F
Sbjct: 1244 PFYFFPQNQVPSWFRIQQLNDTVKMKLPPNMFREKKWMGLAMFAVFSVERNSEFVKDHPF 1303
Query: 1261 TCQISSDEDGRHLMKGGSYSSHS-FLIPLSLSQFSPQILFVTFKPRHVFPYVQDGSNHFY 1320
+ QI DE+G +L + SY+ HS +IPL L S +ILF+ FKPR PYVQD SNH Y
Sbjct: 1304 SWQIDIDENGTYLKRVRSYAFHSPHMIPLQLLWSSSKILFMIFKPRETSPYVQDKSNHLY 1363
Query: 1321 VSFISKATRLRVESCGARLVYQHNVEGLIHTIMDCVLKSGQELFQEYSGKILSDALTTYE 1380
V F++ +T L+VE CGARLVYQHNV+G IH I+DCVLKS Q+ Q YS KIL D L TY+
Sbjct: 1364 VKFLTNSTSLKVELCGARLVYQHNVDGFIHKIVDCVLKSEQKQCQNYSHKILRDLLATYQ 1423
Query: 1381 RLIWIRVPADEPTTSTSSDLSGHHSTNKLLHENFQRPLFKESTQDRYKSKFDAIIHGKEI 1440
+ IW +V TSTS++ + H ST L HE+ QRP FKE QDRYKSKF++IIHGKEI
Sbjct: 1424 QPIWKKV-----ATSTSNNPTQHLSTETLRHESSQRPPFKERIQDRYKSKFNSIIHGKEI 1483
Query: 1441 PKFFTSQNMGNEGTVRIPERLYFAKCEVGVLVCAVVAINKQREKLERISELQSCVKVLDL 1500
P FF S++MGNE T+RIPE + F + VGVL+CAVV I+K EKLERI E QS +KVLDL
Sbjct: 1484 PNFFISRSMGNEATIRIPEIVNFIEYHVGVLMCAVVTISKLIEKLERIPEPQSGIKVLDL 1543
Query: 1501 KCEFGVDSHEVEPEHHLFLPAEALSSQYEPSFIWLSCIPLAEFNVPWMCCDYLKSKVSSN 1560
KCEFGVDSHEV+P+HHL +PA+ L SQ SFIWL IPL+ F++P C+Y +S VSSN
Sbjct: 1544 KCEFGVDSHEVKPKHHLLVPAKVLLSQCRTSFIWLFFIPLSAFHIPLKYCNYFRSMVSSN 1603
Query: 1561 HEELFHVQQCGLHLLYVSERKLVDNLLMT 1582
+EEL VQQCGLHLL+ ERK+VDN L T
Sbjct: 1604 YEELLDVQQCGLHLLHARERKMVDNFLTT 1623
BLAST of Sgr016774 vs. NCBI nr
Match:
XP_022141776.1 (LOW QUALITY PROTEIN: TMV resistance protein N-like [Momordica charantia])
HSP 1 Score: 2114.0 bits (5476), Expect = 0.0e+00
Identity = 1081/1403 (77.05%), Postives = 1184/1403 (84.39%), Query Frame = 0
Query: 1 MEVPSSTPPNRPRMTYDVFISFRGEDTRNNFVGFLHEALRQLGIMTFLDDKKLLIGDNLS 60
MEVPS+ PPNRPRMTYDVFISFRGEDTRNNFVGFL+EAL QLGIMTF+DDKKLLIGD+LS
Sbjct: 1 MEVPSTAPPNRPRMTYDVFISFRGEDTRNNFVGFLYEALCQLGIMTFMDDKKLLIGDDLS 60
Query: 61 ENLVRAIEESRSSIVVLSKDYASSKWCLKELAKIIDCMAETTHRVLPVFYHVDPSNVRHQ 120
E LV+AIEESRSSIVVLSKDYASSKWCLKELAKI+DCM++TTHRVLPVFYHVDPS+VRHQ
Sbjct: 61 EKLVKAIEESRSSIVVLSKDYASSKWCLKELAKIMDCMSDTTHRVLPVFYHVDPSHVRHQ 120
Query: 121 SGSFKKSFDEHETIAHKFIGEQKEKYLEEVQNWRDSMTKVGLLSGEVVTEHSPEVDIVKK 180
SG+F+KSFDEHE A KF G+QKE+Y +EV+NW+D P V
Sbjct: 121 SGNFRKSFDEHEEKAQKFTGQQKEEYWKEVRNWKD-----------------PRV----- 180
Query: 181 ITNQIFERWRPKLVTSDKNLVGMTYRLLKMNVQLGLGLDDVRFLGIVGMGGIGKTTIAKV 240
R KMN+QLGLGLDDVRF+GIVGMGGIGKT IA+V
Sbjct: 181 -------------------------RYKKMNMQLGLGLDDVRFIGIVGMGGIGKTKIAEV 240
Query: 241 VYDCITSKFEGSCFLRVSRGSSKQIELVKLQEQLLSRLFLKENVQIWDEDYGAEMIENQL 300
VYDCI SKFE +CFLRV GSS+QI LV LQEQLLSRLFLKENV+IWDEDYGAEMIE QL
Sbjct: 241 VYDCIKSKFECNCFLRVFGGSSRQINLVSLQEQLLSRLFLKENVRIWDEDYGAEMIEKQL 300
Query: 301 RSRKVLLVLDGVEEKRQVEMLAGSPDWFGPGSRVIITTRNKEVL-QPYEILEYKVDLLDS 360
SRKVLLVLDGVEEK Q+EMLA SPDWFGPGSR+IITTRNKEVL QPYEILE+KV LLD+
Sbjct: 301 CSRKVLLVLDGVEEKEQLEMLARSPDWFGPGSRIIITTRNKEVLHQPYEILEHKVKLLDN 360
Query: 361 DSALQLFCNRAFGTDYVDSNFMNLSKEIVEKLGRLPLALKVIGSYLNNKNGVIWNETLNR 420
D+ALQLFCN AFG ++D+NFMNLSKE+VE RLPLALKVIGSYLN KNGV+W ETLNR
Sbjct: 361 DNALQLFCNHAFGIAHIDNNFMNLSKEMVEXAWRLPLALKVIGSYLNKKNGVVWKETLNR 420
Query: 421 LIKVDEKYLFDVLKISYDGLGAESQKVFLDLACFFNGRRIDKVIKILESFGYNPHSELHL 480
LIKV E+ LF+VLKISYDGLG ESQKVFLDLACFFNGRR+DKVI+ILESFGYNPHSELHL
Sbjct: 421 LIKVKEERLFEVLKISYDGLGVESQKVFLDLACFFNGRRLDKVIEILESFGYNPHSELHL 480
Query: 481 FMERCLIEVSHSKILMHDLILALGQEIVRKESLTQLGKQSRIWLQEDVIRAFTMKHGLKY 540
F+E+CLIE SHSKILMHDLI+ALGQEIVRKESLTQLGKQSRIWLQEDV+RAFT+KH
Sbjct: 481 FIEKCLIEDSHSKILMHDLIVALGQEIVRKESLTQLGKQSRIWLQEDVVRAFTIKH---- 540
Query: 541 TEGIVLNLEKKQKELTLEAKLFADMTCLRILEISNVQLSGNIKSLSNLLGLLNWPGYPSK 600
AKLFADMTCLRILEISNVQLSGNIKSLSNLLGLLNWPGYPSK
Sbjct: 541 ------------------AKLFADMTCLRILEISNVQLSGNIKSLSNLLGLLNWPGYPSK 600
Query: 601 CLPSNFQSGYLLELHLCRSSIVRIWEGKKEFEKLKVINVSDSEYLLETPDFSEVPNLERL 660
CLPSNFQSGYL ELHL S I+RIW+GKKEFE LKVI+VS+SEYLLETPDFSEVP LERL
Sbjct: 601 CLPSNFQSGYLHELHLRHSGILRIWDGKKEFENLKVIDVSNSEYLLETPDFSEVPKLERL 660
Query: 661 VLRNCRSLYHIHPSINSLSRLIFLDLNGCSSLKSFPSNLRCKNLERLVLSKSGVTSSLEI 720
VLRNC LYHIH SINSL LI LDL CSS KSF +NL CKNLE LVLSKSGVTSS E
Sbjct: 661 VLRNCSRLYHIHHSINSLHHLILLDLTNCSSFKSFSTNLGCKNLEHLVLSKSGVTSSPEF 720
Query: 721 KGNMEHLCELRLDGTPIKQLHSSIERLVGLIVLDLRNCIMLSSLPNEIGNLKSLESLLLG 780
+GNMEHL +L LD TPIKQLHSSIE LVGLI+LDLRNCIMLSSLP+EIGNL SLE+L LG
Sbjct: 721 EGNMEHLRKLHLDETPIKQLHSSIEHLVGLILLDLRNCIMLSSLPDEIGNLNSLEALFLG 780
Query: 781 GCVNLDQIPPSLGNAQCLAYLEISETSICRLPPTIHCLKNLKGLNCDGLSRSIWPSLLRP 840
GCVNLDQIPPSLGNAQCL +L+I +TSICR+PPTIHCLKNL+GLNCDGLS SIW SLL P
Sbjct: 781 GCVNLDQIPPSLGNAQCLQFLDIGKTSICRVPPTIHCLKNLEGLNCDGLSCSIWRSLLPP 840
Query: 841 CDILGSDLGISGSKFGLESLEYLTLKGCNLVNEDIPEDLHCFSSLKTLDLSGNDFVRLPE 900
+ILGSDLGI KFGLESLE+L+LK CNLVNEDIPEDLH FSSLKTLDLSGN+FV LPE
Sbjct: 841 DNILGSDLGIPSLKFGLESLEFLSLKDCNLVNEDIPEDLHYFSSLKTLDLSGNNFVWLPE 900
Query: 901 SVNHLKNLRELRLHDCFKLQHLPQLPTNLVPIVSYTESGTPVRSFPIPYQHGKHFILEFI 960
S+NHLKNLREL+LHDCFKLQ LPQLPT+L PI+SYTESG +R+ PIPY H KHFILEFI
Sbjct: 901 SINHLKNLRELKLHDCFKLQQLPQLPTHLEPIISYTESGA-IRNLPIPYHHDKHFILEFI 960
Query: 961 PKTNLKLDPKAFEESILKGVLFSSIDDKAEVLECFDEIREGNLIEIEFYQIKSDANNTYG 1020
PKTNLKLD KAFEESI +G LF S DDKAEVLECFDE REGN+IEI+ YQIK+DAN+ YG
Sbjct: 961 PKTNLKLDLKAFEESISEGALFYSTDDKAEVLECFDETREGNVIEIDQYQIKNDANSIYG 1020
Query: 1021 VVLSACIQCSPNTTPEEVVTFYSKFFIELEMEENPRIGILPVTSKLTSRTRFWMLFIPFH 1080
VVL+ACIQCS N TP EV+TFY KF +ELEME NP+ LP+TSKLTS+TRFWM FIP H
Sbjct: 1021 VVLAACIQCSQN-TPHEVITFYRKFLVELEMEGNPKTLSLPLTSKLTSKTRFWMSFIPSH 1080
Query: 1081 MLPKCRLRFKIVVKGSFPS-VNVQKCGASILSHQNAPKFLANMFNMMYRTCNQNKFCTTI 1140
MLP+CR+ FKIVVKGS PS VNVQKCGA +LSHQNA +FLA MFN +Y T QNKF TTI
Sbjct: 1081 MLPECRVCFKIVVKGSLPSVVNVQKCGAFVLSHQNASRFLAYMFNKVYHTRKQNKFSTTI 1140
Query: 1141 RECNHGSECSCDEPETVDQYCTTQYVENESDSFSLLKRNLKSILQRTYEEENHRSLVYFF 1200
RECNHGS+CSCDEPET+ QYC+TQYVENESDS SLLKR+LKSILQRTYEEE SL YFF
Sbjct: 1141 RECNHGSKCSCDEPETIYQYCSTQYVENESDSLSLLKRSLKSILQRTYEEEKDHSLYYFF 1200
Query: 1201 PQGQVSSWFRIQQPKDTVAVKLPLNLFREKKWMGLAVFAIFSIPGNAEFVHNHIFTCQIS 1260
PQG SWFRIQQPKD VAVKLPL+LFREKKWMGLAVFAIFSIPGN+EFVH+H F QI+
Sbjct: 1201 PQGPAVSWFRIQQPKDRVAVKLPLDLFREKKWMGLAVFAIFSIPGNSEFVHHHTFNFQIT 1260
Query: 1261 SDEDGRHLMKGGSYSSHSFLIPLSLSQFSPQILFVTFKPRHVFPYVQDGSNHFYVSFISK 1320
S+E+ RHLM GGSY S SFLIPLSL Q SPQILFVTF PRHVFPYVQ G +HFYVSFIS
Sbjct: 1261 SNENDRHLMSGGSYLSDSFLIPLSLLQTSPQILFVTFAPRHVFPYVQTGLDHFYVSFISI 1320
Query: 1321 ATRLRVESCGARLVYQHNVEGLIHTIMDCVLKSGQELFQEYSGKILSDALTTYERLIWIR 1380
TRLRVESCGARLVYQHNVEGLI+TIMDCV+KSGQE +Q Y+ IL + LTTY R I IR
Sbjct: 1321 PTRLRVESCGARLVYQHNVEGLINTIMDCVIKSGQECYQNYNQTILRNFLTTYHRPIRIR 1332
Query: 1381 VPADEPT--TSTSSDLSGHHSTN 1400
VPA+ T TSTS++LSG H ++
Sbjct: 1381 VPANPSTTSTSTSTNLSGEHKSS 1332
BLAST of Sgr016774 vs. NCBI nr
Match:
XP_038890520.1 (TMV resistance protein N-like isoform X1 [Benincasa hispida] >XP_038890521.1 TMV resistance protein N-like isoform X1 [Benincasa hispida] >XP_038890522.1 TMV resistance protein N-like isoform X1 [Benincasa hispida] >XP_038890523.1 TMV resistance protein N-like isoform X1 [Benincasa hispida] >XP_038890524.1 TMV resistance protein N-like isoform X1 [Benincasa hispida] >XP_038890525.1 TMV resistance protein N-like isoform X1 [Benincasa hispida] >XP_038890526.1 TMV resistance protein N-like isoform X1 [Benincasa hispida] >XP_038890527.1 TMV resistance protein N-like isoform X1 [Benincasa hispida] >XP_038890528.1 TMV resistance protein N-like isoform X1 [Benincasa hispida] >XP_038890530.1 TMV resistance protein N-like isoform X1 [Benincasa hispida])
HSP 1 Score: 1448.0 bits (3747), Expect = 0.0e+00
Identity = 824/1647 (50.03%), Postives = 1090/1647 (66.18%), Query Frame = 0
Query: 1 MEVPSSTPPNRPRMTYDVFISFRGEDTRNNFVGFLHEALRQLGIMTFLDDKKLLIGDNLS 60
M+ SS+ + P+M YDVFISFRG D R+ F G+L++AL +LGI FLDDK+ LIGD+L
Sbjct: 1 MQSSSSSSLDCPKMNYDVFISFRGRDVRHTFAGYLYDALNRLGIKAFLDDKRFLIGDDL- 60
Query: 61 ENLVRAIEESRSSIVVLSKDYASSKWCLKELAKIIDCMAETTHRVLPVFYHVDPSNVRHQ 120
L + I+ESRS+IVVLS+DYAS+KWCL+EL KI+D M T RVLPVFYH+DPS V+ Q
Sbjct: 61 HGLFQIIDESRSAIVVLSEDYASAKWCLRELTKIMDSMGTTMDRVLPVFYHIDPSVVKDQ 120
Query: 121 SGSFKKSFDEHETIAHKFIGEQ-KEKYLEEVQNWRDSMTKVGLLSGEVVTEHSPEVDIVK 180
SG+FKKSFDEHE A K I +Q KEK L+E+Q+W+ +M K+G +G V+T++S EVDIV
Sbjct: 121 SGTFKKSFDEHEANALKEIDDQEKEKRLKELQSWKSAMKKIGNHTGVVITKNSSEVDIVN 180
Query: 181 KITNQIFERWRPKLVTSDKNLVGMTYRLLKMNVQLGLGLDDVRFLGIVGMGGIGKTTIAK 240
KI NQIF+ WRPKL +KNLVGMT RLL MN+ LGLGLDDVRF+ IVGMGGIGKTTIA+
Sbjct: 181 KIANQIFDVWRPKLEALNKNLVGMTSRLLHMNMHLGLGLDDVRFVAIVGMGGIGKTTIAQ 240
Query: 241 VVYDCITSKFEGSCFLRVSRGSSKQIELVKLQEQLLSRLFLKENVQIWDEDYGAEMIENQ 300
VV+DCI SKF+ CFL + G SKQ LV LQ ++LS++F KE+ +IW E++G EMI+N+
Sbjct: 241 VVFDCILSKFDDCCFLTLPGGDSKQ-SLVSLQREMLSQIFHKEDFRIWHENHGVEMIKNR 300
Query: 301 LRSRKVLLVLDGVEEKRQVEMLAGSPDWFGPGSRVIITTRNKEVLQPY---EILEYKVDL 360
L RKVL+VLDGVEE+RQ+EMLAGS +WFGPGSR+IITTRNK +L + E+ EY V+
Sbjct: 301 LSGRKVLIVLDGVEERRQLEMLAGSAEWFGPGSRIIITTRNKGILNHHNYDEMKEYNVEE 360
Query: 361 LDSDSALQLFCNRAFGTDYVDS-NFMNLSKEIVEKLGRLPLALKVIGSYLNNKNGVIWNE 420
LD DSALQLF AFG+++ ++ +FM+LS E++EK RLPLAL+VIGS+L K IW E
Sbjct: 361 LDHDSALQLFLKHAFGSNHQNNDSFMDLSNEMIEKAKRLPLALRVIGSFLYGKEITIWRE 420
Query: 421 TLNRLIKVDEKYLFDVLKISYDGLGAESQKVFLDLACFFNGRRIDKVIKILESFGYNPHS 480
TL RLIKVDE+ F VLKISYDGLG ESQ+VFLD+ CFFNG+ D+VI+ILESFGY+P S
Sbjct: 421 TLKRLIKVDERSFFHVLKISYDGLGVESQQVFLDITCFFNGKNEDRVIEILESFGYSPKS 480
Query: 481 ELHLFMERCLIEVSHSKILMHDLILALGQEIVRKESLTQLGKQSRIWLQEDVIRAFTMKH 540
E+ L M+RCLIEVSH KIL+HDLIL +G+EIVRKESLTQ KQSRIWL ED+ F KH
Sbjct: 481 EIQLLMQRCLIEVSHKKILVHDLILEMGREIVRKESLTQPEKQSRIWLHEDLYCKFAEKH 540
Query: 541 GLKYTEGIVLNLEKKQKE-LTLEAKLFADMTCLRILEISNVQLSGNIKSLSNLLGLLNWP 600
L + +GIVL+LEK+ +E + L+A+ F++MT LRILEI+NV+LS +I+ LS LL ++NWP
Sbjct: 541 DLMHIQGIVLSLEKEMEESIELDAESFSEMTKLRILEINNVELSEDIEYLSQLLRIINWP 600
Query: 601 GYPSKCLPSNFQSGYLLELHLCRSSIVRIWEGKKEFEKLKVINVSDSEYLLETPDFSEVP 660
GYPSK LP FQS YL EL L S I+R+W+GKK F KLKVI+VS+SE+L TPDFS VP
Sbjct: 601 GYPSKSLPPMFQSRYLFELLLPHSHILRLWDGKKRFPKLKVIDVSNSEHLRVTPDFSGVP 660
Query: 661 NLERLVLRNCRSLYHIHPSINSLSRLIFLDLNGCSSLKSFPSNLRCKNLERLVLSKSGVT 720
NLERLVL NC L IHPSINSLS+LI LDL GC LK FP N+RCKNL+ L LS +G+
Sbjct: 661 NLERLVLSNCVKLCEIHPSINSLSKLILLDLEGCGDLKHFPPNIRCKNLQTLKLSGTGLE 720
Query: 721 SSLEIKGNMEHLCELRLDGTPIKQLHSSIERLVGLIVLDLRNCIMLSSLPNEIGNLKSLE 780
+S EI GNMEHL L LD + I QLH SI L GL++LDL +C+ LSSLP EIGNLKSL+
Sbjct: 721 TSPEIGGNMEHLTHLHLDESNITQLHPSIGHLTGLVLLDLSSCLGLSSLPCEIGNLKSLK 780
Query: 781 SLLLGGCVNLDQIPPSLGNAQCLAYLEISETSICRLPPT-IHCLKNLKGLNCDGLSRSIW 840
+LLL C LDQIP SL NA+ L L ISETSI LP + IHCLKNL+ L C+GLS IW
Sbjct: 781 TLLLKYCEKLDQIPKSLANAESLETLCISETSITHLPSSIIHCLKNLETLECEGLSCGIW 840
Query: 841 PSLLRPCDILGSDLGISGSKFGLESLEYLTLKGCNLVNEDIPEDLHCFSSLKTLDLSGND 900
S+L +I + GL L+ L L GC L++EDIPEDLH FSSL+TLDLS N+
Sbjct: 841 KSMLPQFNIHQT------VSTGLGCLKTLNLMGCKLMDEDIPEDLHFFSSLETLDLSYNN 900
Query: 901 FVRLPESVNHLKNLRELRLHDCFKLQHLPQLPTNLVPIVSYTESGTPVRSFP-------- 960
F LP S++HL L+ L L+ C +L+ LP+LP +L + G RS
Sbjct: 901 FTTLPVSLSHLNKLKTLNLNFCTELKDLPKLPDSLQYV-----GGIDCRSMSEQYYNKIL 960
Query: 961 -IPYQHGKHFILEFIPKT---NLKLDPKAFEESIL--KGVLFSSIDDK--------AEVL 1020
IP G L FI ++ N++ F+ SI + + I++K ++L
Sbjct: 961 LIPSSSGHQLYLNFIIRSKDANVECAMNEFQHSIFTRRSFELNIIEEKPSTIIHKDVDML 1020
Query: 1021 ECFDEIREGNLIEIEFYQIKS------------DANNTYGVVLSACIQCSPNTTPEEVVT 1080
E F +I EGN I I++ Q S D +N GV LS I+ N +
Sbjct: 1021 EWFGQINEGNWINIQYEQEFSISKPLNIMYEDVDLSNVCGVFLSTNIEFPQNLDHLAL-- 1080
Query: 1081 FYSKFFIELEMEENPRIGILPVTSKLTSRTR-FWMLFIPFHMLPKCRLRFK--IVVKGSF 1140
+F + E++ G + TR FW+ +IP M L + +K +
Sbjct: 1081 --GRFSVSFEIDGKCSGGTMGYEMSQFKATRFFWVAYIPIWMFIHHSLMVQRCCSIKATI 1140
Query: 1141 ---------PSVNVQKCG-ASILSHQNAPKFLANMFNMMYRTCNQNKFCTTIRECN-HGS 1200
V ++ CG +S+LS N ++LA +F Y C++ FCT IR+ N H S
Sbjct: 1141 SYFCDHIDASKVKIKACGVSSLLSWPNVSEYLAKLFAKRY--CSKRNFCTMIRQHNDHNS 1200
Query: 1201 ECSCDEPETV-DQYCTTQYVENESDSFSLLKRNLKSILQRTYEEENHRSLVYFFPQGQVS 1260
EC CDE E D + + Y DS LL+ NL++IL +EE+ + YFFPQ +
Sbjct: 1201 ECRCDELEVAKDDF--SSYTFESDDSTFLLRMNLRAILGIMFEEKKRYYMKYFFPQTKFF 1260
Query: 1261 SWFRIQQPKDTVAVKLPLNLFREKKWMGLAVFAIFSIPGNAEFVHNHIFTCQISSDEDGR 1320
WF+ Q KD VAVK+P+N+ ++KKWMGLA+F +FSI A + F +I + E
Sbjct: 1261 GWFKNQNKKDKVAVKIPVNIDKDKKWMGLAMFVVFSISKKASC---YCFEYEIQTKE--- 1320
Query: 1321 HLMKGGSYSSHSFLIPLSLSQFSPQILFVTFKPRH-VFPYVQ---DGSNHFYVSFISKAT 1380
K S HS IP ++++S QILF+ F+PR+ +PY + SNH Y++F + +
Sbjct: 1321 ---KIISTQRHS--IPKEVAEYSNQILFIVFEPRYNWYPYDELKSSSSNHVYINFNTNSE 1380
Query: 1381 RLRVESCGARLVYQHNVEGLIHTIMDCVLKSGQELFQEYSGKILSDALTTYE-RLIWIRV 1440
R+RVE CGARLVYQ NVEGLIHTI++C++KSG EL++ Y+ +I+ L I +
Sbjct: 1381 RMRVELCGARLVYQQNVEGLIHTIVNCIVKSGDELYEYYNQQIVEYHLKMINTHWYTISL 1440
Query: 1441 PADEPT-----TSTSSDLSGHHSTNKLLHENFQRPLFKESTQDRYKSKFDAIIHGKEIPK 1500
P + P T+ S+ ++ S LLH +F P F +S Q+R+ SKFD ++ G +IPK
Sbjct: 1441 PRNNPVKNQKPTTASTCIASSLSVEHLLHGSFPHPFFHKSIQERFGSKFDLLLRGDKIPK 1500
Query: 1501 FFTSQNMGNEGTVRIPERLYFAKCEVGVLVCAVVAINKQREKL-ERISELQSCVKVLDLK 1560
FFTSQ+ GN +++P+ L + VG+ VCA+V ++K+R KL + I E + KV+DL
Sbjct: 1501 FFTSQSRGNMTEIKLPQYLENFRDSVGLAVCALVVVDKKRRKLNDIIPEQERYTKVVDLI 1560
Query: 1561 CEFGVDSHEVEPEHHLFLPAEALSSQYEPSFIWLSCIPLAEFNVPWMCCDYLKSKVSSNH 1581
C+F VDS+++ EH F+ + L S+Y FIWLS IPL F++ W C + + ++
Sbjct: 1561 CKFKVDSYQILQEHCHFVSQQKLLSEYASQFIWLSYIPLNGFDINWHYCTQFQITLETSC 1615
BLAST of Sgr016774 vs. NCBI nr
Match:
KAA0039330.1 (TMV resistance protein N-like [Cucumis melo var. makuwa] >TYK00512.1 TMV resistance protein N-like [Cucumis melo var. makuwa])
HSP 1 Score: 1431.4 bits (3704), Expect = 0.0e+00
Identity = 808/1651 (48.94%), Postives = 1090/1651 (66.02%), Query Frame = 0
Query: 1 MEVPSSTPPNRPRMTYDVFISFRGEDTRNNFVGFLHEALRQLGIMTFLDDKKLLIGDNLS 60
M+ SS+ +RP+M YDVFISFRG D R+ F G+L++AL +LGI FLD+K+ LIGD+L
Sbjct: 1 MQSSSSSSLDRPKMNYDVFISFRGRDVRHTFAGYLYDALNRLGIKAFLDNKRFLIGDDL- 60
Query: 61 ENLVRAIEESRSSIVVLSKDYASSKWCLKELAKIIDCMAETTHRVLPVFYHVDPSNVRHQ 120
+L + I+ESRS+IVVLS+DYAS+KWCL+EL KI+D M + RVLPVFYH+DPS V+ Q
Sbjct: 61 HDLFKIIDESRSAIVVLSEDYASAKWCLRELTKIMDSMGTSMERVLPVFYHIDPSIVKDQ 120
Query: 121 SGSFKKSFDEHETIAHKFI-GEQKEKYLEEVQNWRDSMTKVGLLSGEVVTEHSPEVDIVK 180
SG+FK SFDEHE A K I ++KEK L+E+QNW++++ K+G +G V+T++S EVDIV
Sbjct: 121 SGTFKTSFDEHEANALKEIDNQEKEKRLKELQNWKNALKKIGNHTGVVITKNSSEVDIVN 180
Query: 181 KITNQIFERWRPKLVTSDKNLVGMTYRLLKMNVQLGLGLDDVRFLGIVGMGGIGKTTIAK 240
KI +QIF+ WRPKL +KNLVGMT RLL MN+ LGLGLDDVRF+ IVGMGGIGKTTIA+
Sbjct: 181 KIASQIFDAWRPKLEALNKNLVGMTSRLLHMNMHLGLGLDDVRFVAIVGMGGIGKTTIAQ 240
Query: 241 VVYDCITSKFEGSCFLRVSRGSSKQIELVKLQEQLLSRLFLKENVQIWDEDYGAEMIENQ 300
VV+DCI SKF+ CFL + G SKQ LV LQ ++LS++F KE+ +IW E++G EMI+N+
Sbjct: 241 VVFDCILSKFDDCCFLTLPGGDSKQ-SLVSLQREMLSQIFHKEDFRIWHENHGVEMIKNR 300
Query: 301 LRSRKVLLVLDGVEEKRQVEMLAGSPDWFGPGSRVIITTRNKEVL---QPYEILEYKVDL 360
L RKVL+VLDGVEE+RQ+EMLAGS DWFGPGSR+IITTRNK +L E+ EY V+
Sbjct: 301 LSGRKVLIVLDGVEERRQLEMLAGSTDWFGPGSRIIITTRNKGLLCHPNYDEMKEYNVEE 360
Query: 361 LDSDSALQLFCNRAFGTDYVD-SNFMNLSKEIVEKLGRLPLALKVIGSYLNNKNGVIWNE 420
LD DSALQLF AFG+++ + +FM+LS EIVEK RLPLAL+VIGS L K IW E
Sbjct: 361 LDHDSALQLFLKHAFGSNHQNKDSFMDLSNEIVEKAKRLPLALRVIGSSLYGKEITIWRE 420
Query: 421 TLNRLIKVDEKYLFDVLKISYDGLGAESQKVFLDLACFFNGRRIDKVIKILESFGYNPHS 480
TL RLIKVDE+ FD+LKISYDGLG ESQ+VFLD+ CFFNG+ D+V +ILESFGY+P+S
Sbjct: 421 TLKRLIKVDERNFFDILKISYDGLGVESQQVFLDITCFFNGKNEDRVNEILESFGYSPNS 480
Query: 481 ELHLFMERCLIEVSHSKILMHDLILALGQEIVRKESLTQLGKQSRIWLQEDVIRAFTMKH 540
EL L M+RCLIEVSH KIL+HDLIL +G+EIVRKESLTQ KQSRIWL ED+ F KH
Sbjct: 481 ELQLLMQRCLIEVSHKKILVHDLILEMGREIVRKESLTQPEKQSRIWLHEDLYCRFAEKH 540
Query: 541 GLKYTEGIVLNLEKKQKE-LTLEAKLFADMTCLRILEISNVQLSGNIKSLSNLLGLLNWP 600
L + +GIVL+LEK+ +E + L+A+ F++MT LRILEI+NV+L +I+ LS LL ++NW
Sbjct: 541 DLMHIQGIVLSLEKEMEESIELDAESFSEMTKLRILEINNVELDEDIEYLSPLLRIINWL 600
Query: 601 GYPSKCLPSNFQSGYLLELHLCRSSIVRIWEGKKEFEKLKVINVSDSEYLLETPDFSEVP 660
GYPSK LP FQS YL EL L S ++R+W+GKK F KLK+I+VS+SE+L TPDFS VP
Sbjct: 601 GYPSKSLPPTFQSRYLFELLLPHSHLLRVWDGKKRFPKLKLIDVSNSEHLRVTPDFSGVP 660
Query: 661 NLERLVLRNCRSLYHIHPSINSLSRLIFLDLNGCSSLKSFPSNLRCKNLERLVLSKSGVT 720
NLERLVL NC L IHPSINSL++LI LDL GC LK FP+N+RCKNL+ L LS +G+
Sbjct: 661 NLERLVLCNCVRLCEIHPSINSLNKLILLDLEGCGDLKHFPANIRCKNLQTLKLSGTGLE 720
Query: 721 SSLEIKGNMEHLCELRLDGTPIKQLHSSIERLVGLIVLDLRNCIMLSSLPNEIGNLKSLE 780
EI G+MEHL L LDG+ I LH SI L GL+ LDL C+ LSSLP EIGNLKSL+
Sbjct: 721 IFPEI-GHMEHLTHLHLDGSKITHLHPSIGYLTGLVFLDLSTCLGLSSLPFEIGNLKSLK 780
Query: 781 SLLLGGCVNLDQIPPSLGNAQCLAYLEISETSICRLPPT-IHCLKNLKGLNCDGLSRSIW 840
+LLL C LD+IPPSL NA+ L L ISETSI +P + IHCLKNL+ L+C+GLSR IW
Sbjct: 781 TLLLKYCKRLDKIPPSLANAESLETLSISETSITHVPSSIIHCLKNLETLDCEGLSRGIW 840
Query: 841 PSLLRPCDILGSDLGISGSKFGLESLEYLTLKGCNLVNEDIPEDLHCFSSLKTLDLSGND 900
SLL +I + GL L+ L L GC L++EDIPEDLHCFSSL+ LDLS N+
Sbjct: 841 KSLLPQLNINQT------ITTGLGCLKALNLMGCKLMDEDIPEDLHCFSSLEALDLSYNN 900
Query: 901 FVRLPESVNHLKNLRELRLHDCFKLQHLPQLPTNLVPIVSYTESGTPVRSFP-------- 960
F LP+S++HLK L+ L L+ C +L+ LP+LP +L + G RS
Sbjct: 901 FTTLPDSLSHLKKLKTLNLNYCTELKDLPKLPESLQYV-----GGVDCRSMSEQYYNKIL 960
Query: 961 -IPYQHGKHFILEF-IPKTNLKLD------------PKAFEESILKGVLFSSIDDKAEVL 1020
IP G L F IP + ++ ++FE+SI++ + + D ++
Sbjct: 961 LIPSSSGHQLYLTFIIPSKDADVECAMNEFQHSIFTRRSFEQSIIEEQPSTIVHDTVDMF 1020
Query: 1021 ECFDEIREGNLIEIEFYQIKS------------DANNTYGVVLSACIQCSPNTTPEEVVT 1080
+ F +I EGN I++ Q S D +N GV LS I+ N +
Sbjct: 1021 QWFGQINEGNWTNIQYEQEFSISKPLNIMYEDVDLSNVCGVFLSTNIEFPQNLNHLAIGR 1080
Query: 1081 FYSKFFIELEMEENPRIGILPVTSKLTSRTRFWMLFIPFHMLPKCRLRFK----IVVKGS 1140
F F I+ + + S+ + FW+ +IP M+ L + + V S
Sbjct: 1081 FLVSFGIDGKCSGGT---MNYEMSQFKAARFFWVAYIPIWMIKDHSLMVQRCCSVKVTIS 1140
Query: 1141 F-------PSVNVQKCG-ASILSHQNAPKFLANMFNMMYRTCNQNKFCTTIRECN-HGSE 1200
+ V ++ CG +S+LS N ++LA +F R C++ F T IR+ N H +E
Sbjct: 1141 YCCDHIDASKVKIKACGVSSMLSWPNVAEYLAKLFT--ERFCSKRNFYTMIRQHNDHQTE 1200
Query: 1201 CSCDEPET-VDQYCTTQYVENESDSFSLLKRNLKSILQRTYEEENHRSLVYFFPQGQVSS 1260
C CDE E D + ++ + N DS LL++NL++IL +EE+ + YFFP ++
Sbjct: 1201 CRCDELEVGKDDFSSSTFESN--DSTFLLRKNLRAILGVMFEEKKRYYMKYFFPHTKIFG 1260
Query: 1261 WFRIQQPKDTVAVKLPLNLFREKKWMGLAVFAIFSIPGNAEFVHNHIFTCQISSDED--- 1320
WF+ Q KD VAVK+P+N+ +++KWMGLA+F +FSI +E + F +I + E+
Sbjct: 1261 WFKNQNKKDKVAVKIPVNIEKDRKWMGLAMFVVFSI---SEKASCYCFEYEIQTKENIIS 1320
Query: 1321 -GRHLMKGGSYSSHSFLIPLSLSQFSPQILFVTFKPRH-VFPYVQ---DGSNHFYVSFIS 1380
RH + H S QILFV F+PR+ +PY + SNH Y++F +
Sbjct: 1321 TQRHFISTDQVLEH-----------SNQILFVAFEPRYNWYPYDELKSSSSNHVYINFNT 1380
Query: 1381 KATRLRVESCGARLVYQHNVEGLIHTIMDCVLKSGQELFQEYSGKILSDALTTYERLIWI 1440
R+RVE CGARLVYQ NVEGLIHTI++C+ +SG EL++ Y+ I+ L T+ W
Sbjct: 1381 NGARMRVEFCGARLVYQQNVEGLIHTILNCIAESGDELYEHYNQYIVESHL-TFINTHWY 1440
Query: 1441 RVP-------ADEPTTSTSSDLSGHHSTNKLLHENFQRPLFKESTQDRYKSKFDAIIHGK 1500
+ ++P+T+ S+ + S LL+ +F P F +S Q+R++SKFD ++HG
Sbjct: 1441 TLSFRRNNSVKNQPSTAASTCTASSLSVEHLLYGSFPHPFFHKSLQERFRSKFDLLLHGD 1500
Query: 1501 EIPKFFTSQNMGNEGTVRIPERLYFAKCEVGVLVCAVVAINKQREKLERI-SELQSCVKV 1560
+IPKFF++Q+ GN +++P+ L + VG+ VCA+V ++K+R KL I E + KV
Sbjct: 1501 KIPKFFSNQSGGNMTEIKLPQYLEKFRESVGLAVCALVVVDKKRRKLNEIMPERERYTKV 1560
Query: 1561 LDLKCEFGVDSHEVEPEHHLFLPAEALSSQYEPSFIWLSCIPLAEFNVPWMCCDYLKSKV 1581
+DL C+F VDS+++ PEH F + L S+Y F+WLS IPL FN+ W C + +
Sbjct: 1561 VDLICKFKVDSYQIMPEHCHFTSQQKLLSEYASQFLWLSYIPLHGFNINWHYCTQFEVAL 1615
BLAST of Sgr016774 vs. ExPASy Swiss-Prot
Match:
V9M2S5 (Disease resistance protein RPV1 OS=Vitis rotundifolia OX=103349 GN=RPV1 PE=1 SV=1)
HSP 1 Score: 581.6 bits (1498), Expect = 2.6e-164
Identity = 384/1005 (38.21%), Postives = 544/1005 (54.13%), Query Frame = 0
Query: 5 SSTPPNRPR-MTYDVFISFRGEDTRNNFVGFLHEALRQLGIMTFLDDKKLLIGDNLSENL 64
SS+ P+ PR TYDVF+SFRGEDTR NF L+ AL + GI TF DD +L G+ ++ L
Sbjct: 12 SSSTPSIPRTTTYDVFLSFRGEDTRYNFTDHLYSALGRRGIRTFRDD-RLRRGEAIAPEL 71
Query: 65 VRAIEESRSSIVVLSKDYASSKWCLKELAKIIDCMAETTHRVLPVFYHVDPSNVRHQSGS 124
++AIEESRSS++V S++YA S+WCL EL KI++C + H V P+FYHVDPS+VR Q GS
Sbjct: 72 LKAIEESRSSVIVFSENYAHSRWCLDELVKIMECQKDLGHAVFPIFYHVDPSHVRKQEGS 131
Query: 125 FKKSFDEHETIAHKFIGEQKEKYLEEVQNWRDSMTKVGLLSGEVVTEHSPEVDIVKKITN 184
F ++F +E E + +++ WR ++T+ LSG + + E + +K+ITN
Sbjct: 132 FGEAFAGYE-----------ENWKDKIPRWRTALTEAANLSGWHLLDDRYESNQIKEITN 191
Query: 185 QIFERWRPKLVTSDKNLVGMTYRLLKMNVQLGLGLDDVRFLGIVGMGGIGKTTIAKVVYD 244
IF + + K + NLVG+ + +M ++L L DVR +GI G+GGIGKTTIAKV+Y+
Sbjct: 192 SIFRQLKCKRLDVGANLVGIDSHVKEMILRLHLESSDVRMVGIYGVGGIGKTTIAKVIYN 251
Query: 245 CITSKFEGSCFLRVSRGSSKQIELVKLQEQLLSRLFLKENVQ-IWDEDYGAEMIENQLRS 304
++ +FE FL R S L LQ QLL + E Q I + A MI++ L S
Sbjct: 252 ELSCEFEYMSFLENIREGSNPQVLFHLQNQLLGDILEGEGSQNISSVAHRASMIKDILLS 311
Query: 305 RKVLLVLDGVEEKRQVEMLAGSPDWFGPGSRVIITTRNKEVLQPYEILE-YKVDLLDSDS 364
R+V +VLD V++ Q+E L G +W G GSRVIITTRNK VL E+ + Y+V+ L+ +
Sbjct: 312 RRVFIVLDDVDDLSQLEYLLGHREWLGEGSRVIITTRNKHVLAVQEVDDLYEVEGLNFEE 371
Query: 365 ALQLFCNRAFGTDYVDSNFMNLSKEIVEKLGRLPLALKVIGSYLNNKNGVIWNETLNRLI 424
A +LF AF + S++ NL+ +V LPLALKV+GS L K W L +L
Sbjct: 372 ACELFSLYAFKQNLPKSDYRNLTCRVVGYCQGLPLALKVLGSLLCKKTIPQWEGELKKLD 431
Query: 425 KVDEKYLFDVLKISYDGLGAESQKVFLDLACFFNGRRIDKVIKILESFGYNPHSELHLFM 484
+ + VLK SYDGL + +FLDLACFF G D V++IL+ + + +
Sbjct: 432 SEPKADIHKVLKRSYDGLDRIDKNIFLDLACFFKGEGRDFVLRILDGCDFPAETGISNLN 491
Query: 485 ERCLIEVSHSKILMHDLILALGQEIVRKESLTQLGKQSRIWLQEDVIRAFTMKHGLKYTE 544
+ CLI + +++I MHDLI +G EIVR+ + K SR+W D RA T G+K E
Sbjct: 492 DLCLITLPYNQICMHDLIQQMGWEIVRENFPVEPNKWSRLWDPCDFERALTADEGIKSVE 551
Query: 545 GIVLNLEKKQKELTLEAKLFADMTCLRILEI----------------------------S 604
+ L+L K K + + +FA MT LR+L++ S
Sbjct: 552 TMSLDL-SKLKRVCSNSNVFAKMTKLRLLKVYSSSDIDSAHGDSDEDIEEVYDVVMKDAS 611
Query: 605 NVQLSGNIKSLSNLLGLLNWPGYPSKCLPSNFQSGYLLELHLCRSSIVRIWEGKKEFEKL 664
+QL + K S L L W GYP LP NF G L+ELHL S+I ++W+G K+ E+L
Sbjct: 612 KMQLGQSFKFPSYELRYLRWDGYPLDSLPLNFDGGKLVELHLKCSNIKQLWQGHKDLERL 671
Query: 665 KVINVSDSEYLLETPDFSEVPNLERLVLRNCRSLYHIHPSINSLSRLIFLDLNGCSSLKS 724
KVI++S S L + +FS +PNLERL L C SL IHPS+ ++ +L L L C+ LK+
Sbjct: 672 KVIDLSYSRKLSQMSEFSSMPNLERLCLSGCVSLIDIHPSVGNMKKLTTLSLRSCNKLKN 731
Query: 725 FPSNL-RCKNLERLVLSKSGVTSSLEIK-GNMEHLCELRLDGTPIKQLHSSIERLVGLIV 784
P ++ ++LE L LS K GNM+ L EL L T IK L SI L L
Sbjct: 732 LPDSIGDLESLESLYLSNCSKFEKFPEKGGNMKSLTELDLKNTAIKDLPDSIGDLESLES 791
Query: 785 LDLRNC-----------------------IMLSSLPNEIGNLKSLESLLLGGCVNLDQIP 844
L L NC + LP+ IG+L+SLE L L C ++ P
Sbjct: 792 LYLSNCSKFEKFPEKGGNMKSLTELDLKNTAIKDLPDSIGDLESLEILNLSDCAKFEKFP 851
Query: 845 PSLGNAQCLAYLEISETSICRLPPTIHCLKNLKGLNCDGLSR-SIWP----SLLRPCDIL 904
GN + L L++ T+I LP +I LK+LK L+ S+ +P ++ R ++
Sbjct: 852 EKGGNMKSLKELDLQNTAIKDLPDSIGDLKSLKYLSLSDCSKFEKFPEKGGNMKRLLQLI 911
Query: 905 GSDLGIS---GSKFGLESLEYLTLKGCNLVNEDIPEDLHCFSSLKTLDLSGNDFVRLPES 946
S+ I S LESL+YL L C+ E PE SL LDL LP+S
Sbjct: 912 LSNTAIKDLPDSIGDLESLKYLYLSDCSKF-EKFPEKGGNMKSLTELDLKNTAIKDLPDS 971
BLAST of Sgr016774 vs. ExPASy Swiss-Prot
Match:
Q40392 (TMV resistance protein N OS=Nicotiana glutinosa OX=35889 GN=N PE=1 SV=1)
HSP 1 Score: 544.7 bits (1402), Expect = 3.6e-153
Identity = 340/935 (36.36%), Postives = 513/935 (54.87%), Query Frame = 0
Query: 13 RMTYDVFISFRGEDTRNNFVGFLHEALRQLGIMTFLDDKKLLIGDNLSENLVRAIEESRS 72
R +YDVF+SFRGEDTR F L+E L GI TF DDK+L G + L +AIEES+
Sbjct: 9 RWSYDVFLSFRGEDTRKTFTSHLYEVLNDKGIKTFQDDKRLEYGATIPGELCKAIEESQF 68
Query: 73 SIVVLSKDYASSKWCLKELAKIIDCMAETTHRVLPVFYHVDPSNVRHQSGSFKKSFDEHE 132
+IVV S++YA+S+WCL EL KI++C V+P+FY VDPS+VR+Q SF K+F+EHE
Sbjct: 69 AIVVFSENYATSRWCLNELVKIMECKTRFKQTVIPIFYDVDPSHVRNQKESFAKAFEEHE 128
Query: 133 TIAHKFIGEQKEKYLEEVQNWRDSMTKVGLLSGEVVTEHSPEVDIVKKITNQIFERWRPK 192
T + + +E +Q WR ++ + L G + D +++I +QI +
Sbjct: 129 T--------KYKDDVEGIQRWRIALNEAANLKGSCDNRDKTDADCIRQIVDQISSKLCKI 188
Query: 193 LVTSDKNLVGMTYRLLKMNVQLGLGLDDVRFLGIVGMGGIGKTTIAKVVYDCITSK---- 252
++ +N+VG+ L K+ L +G++ VR +GI GMGG+GKTTIA+ ++D + +
Sbjct: 189 SLSYLQNIVGIDTHLEKIESLLEIGINGVRIMGIWGMGGVGKTTIARAIFDTLLGRMDSS 248
Query: 253 --FEGSCFLRVSRGSSKQIELVKLQEQLLSRLFLKENVQIWDEDYGAEMIENQLRSRKVL 312
F+G+CFL+ + + + + LQ LLS L L+E +E+ G + ++LRS+KVL
Sbjct: 249 YQFDGACFLKDIKENKRGMH--SLQNALLSEL-LREKANYNNEEDGKHQMASRLRSKKVL 308
Query: 313 LVLDGVEEK-RQVEMLAGSPDWFGPGSRVIITTRNKEVLQPYEILEYKVDLLDSDSALQL 372
+VLD ++ K +E LAG DWFG GSR+IITTR+K +++ +I+ Y+V L ++QL
Sbjct: 309 IVLDDIDNKDHYLEYLAGDLDWFGNGSRIIITTRDKHLIEKNDII-YEVTALPDHESIQL 368
Query: 373 FCNRAFGTDYVDSNFMNLSKEIVEKLGRLPLALKVIGSYLNNKNGVIWNETLNRLIKVDE 432
F AFG + + NF LS E+V LPLALKV GS L+N W + +
Sbjct: 369 FKQHAFGKEVPNENFEKLSLEVVNYAKGLPLALKVWGSLLHNLRLTEWKSAIEHMKNNSY 428
Query: 433 KYLFDVLKISYDGLGAESQKVFLDLACFFNGRRIDKVIKILESFGYNPHSELHLFMERCL 492
+ D LKISYDGL + Q++FLD+ACF G D +++ILES L + +++ L
Sbjct: 429 SGIIDKLKISYDGLEPKQQEMFLDIACFLRGEEKDYILQILESCHIGAEYGLRILIDKSL 488
Query: 493 IEVS-HSKILMHDLILALGQEIVRKESLTQLGKQSRIWLQEDVIRAFTMKHGLKYTEGIV 552
+ +S ++++ MHDLI +G+ IV + G++SR+WL ++V + G E I
Sbjct: 489 VFISEYNQVQMHDLIQDMGKYIVNFQK--DPGERSRLWLAKEVEEVMSNNTGTMAMEAIW 548
Query: 553 LNLEKKQKELTLEAKLFADMTCLRILEISNVQLSGNIKSLSNLLGLLNWPGYPSKCLPSN 612
++ L + +M LR+ + I L N L YP + PS
Sbjct: 549 VS--SYSSTLRFSNQAVKNMKRLRVFNMGRSSTHYAIDYLPNNLRCFVCTNYPWESFPST 608
Query: 613 FQSGYLLELHLCRSSIVRIWEGKKEFEKLKVINVSDSEYLLETPDFSEVPNLERLVLRNC 672
F+ L+ L L +S+ +W K L+ I++S S+ L TPDF+ +PNLE + L C
Sbjct: 609 FELKMLVHLQLRHNSLRHLWTETKHLPSLRRIDLSWSKRLTRTPDFTGMPNLEYVNLYQC 668
Query: 673 RSLYHIHPSINSLSRLIFLDLNGCSSLKSFPSNLRCKNLERL----VLSKSGVTSSLEIK 732
+L +H S+ S++I L LN C SLK FP C N+E L + S + EI
Sbjct: 669 SNLEEVHHSLGCCSKVIGLYLNDCKSLKRFP----CVNVESLEYLGLRSCDSLEKLPEIY 728
Query: 733 GNMEHLCELRLDGTPIKQLHSSI----ERLVGLIVLDLRNCIMLSSLPNEIGNLKSLESL 792
G M+ ++ + G+ I++L SSI + L++ +++N L +LP+ I LKSL SL
Sbjct: 729 GRMKPEIQIHMQGSGIRELPSSIFQYKTHVTKLLLWNMKN---LVALPSSICRLKSLVSL 788
Query: 793 LLGGCVNLDQIPPSLGNAQCLAYLEISETSICRLPPTIHCLKNLKGLNCDGLSRSI---W 852
+ GC L+ +P +G+ L + S+T I R P +I L L L G + +
Sbjct: 789 SVSGCSKLESLPEEIGDLDNLRVFDASDTLILRPPSSIIRLNKLIILMFRGFKDGVHFEF 848
Query: 853 PSLLRPCDILGSDLGISGSKFGLESLEYLTLKGCNLVNEDIPEDLHCFSSLKTLDLSGND 912
P + GL SLEYL L CNL++ +PE++ SSLK LDLS N+
Sbjct: 849 PPVAE----------------GLHSLEYLNLSYCNLIDGGLPEEIGSLSSLKKLDLSRNN 904
Query: 913 FVRLPESVNHLKNLRELRLHDCFKLQHLPQLPTNL 929
F LP S+ L L+ L L DC +L LP+LP L
Sbjct: 909 FEHLPSSIAQLGALQSLDLKDCQRLTQLPELPPEL 904
BLAST of Sgr016774 vs. ExPASy Swiss-Prot
Match:
V9M398 (Disease resistance protein RUN1 OS=Vitis rotundifolia OX=103349 GN=RUN1 PE=1 SV=1)
HSP 1 Score: 540.4 bits (1391), Expect = 6.7e-152
Identity = 376/1013 (37.12%), Postives = 534/1013 (52.71%), Query Frame = 0
Query: 5 SSTPPNRPR-MTYDVFISFRGEDTRNNFVGFLHEALRQLGIMTFLDDKKLLIGDNLSENL 64
SS+ P+ PR +TYDVF+SFRGEDTR NF L+ AL + GI TF DD KL G+ ++ L
Sbjct: 15 SSSTPSIPRTITYDVFLSFRGEDTRFNFTDHLYSALGRRGIRTFRDD-KLRRGEAIAPEL 74
Query: 65 VRAIEESRSSIVVLSKDYASSKWCLKELAKIIDC---MAETTHRVLPVFYHVDPSNVRHQ 124
++AIEESRSS++V S++YA S+WCL EL KI++C + H V P+FYHVDPS+VR Q
Sbjct: 75 LKAIEESRSSVIVFSENYARSRWCLDELVKIMECHKDKKDPGHAVFPIFYHVDPSHVRKQ 134
Query: 125 SGSFKKSFDEHETIAHKFIGEQKEKYLEEVQNWRDSMTKVGLLSGEVVTEHSPEVDIVKK 184
GSF ++F + E +++ WR ++T+ LSG + + E + +K+
Sbjct: 135 EGSFGEAFAGY-----------GENLKDKIPRWRTALTEAANLSGWPL-QDGYESNQIKE 194
Query: 185 ITNQIFERWRPKLVTSDKNLVGMTYRLLKMNVQLGLGLDDVRFLGIVGMGGIGKTTIAKV 244
IT+ IF R + K + + NLVG+ + +M +L + DVR +G+ G+GGIGKTTIAKV
Sbjct: 195 ITDSIFRRLKCKRLDAGANLVGIDSHVKEMIWRLHMESSDVRMVGMYGVGGIGKTTIAKV 254
Query: 245 VYDCITSKFEGSCFLRVSRGSSKQIELVKLQEQLLSRLFLKENVQ-IWDEDYGAEMIENQ 304
+Y+ ++ +FE FL R + LQ QLL + E Q I +GA MI++
Sbjct: 255 IYNELSREFEYMSFLENIREKFNTQGVSPLQNQLLDDILKGEGSQNINSVAHGASMIKDI 314
Query: 305 LRSRKVLLVLDGVEEKRQVEMLAGSPDWFGPGSRVIITTRNKEVLQPYEILE-YKVDLLD 364
L S+ V +VLD V+++ Q+E L +W G GSRVIITTRNK VL ++ + Y+V L+
Sbjct: 315 LSSKIVFIVLDDVDDQSQLEYLLRHREWLGEGSRVIITTRNKHVLDVQKVDDLYEVKGLN 374
Query: 365 SDSALQLFCNRAFGTDYVDSNFMNLSKEIVEKLGRLPLALKVIGSYLNNKNGVIWNETLN 424
+ A +LF AF + S++ NLS +V LPLALKV+G L K W L
Sbjct: 375 FEEACELFSLYAFEQNLPKSDYRNLSHRVVGYCQGLPLALKVLGCLLLKKTIPEWESELR 434
Query: 425 RLIKVDEKYLFDVLKISYDGLGAESQKVFLDLACFFNGRRIDKVIKILESFGYNPHSELH 484
+L + E + VLK SYDGLG + +FLD+ACFF G D V KIL++ ++ +
Sbjct: 435 KLDREPEAEILSVLKRSYDGLGRTEKSIFLDVACFFKGEDRDFVSKILDACDFHAEIGIK 494
Query: 485 LFMERCLIEVSHSKILMHDLILALGQEIVRKESLTQLGKQSRIWLQEDVIRAFTMKHGLK 544
++CLI + +++I MHDLI +G EIVR++ + K SR+W D RA T G+K
Sbjct: 495 NLNDKCLITLQYNRIRMHDLIQQMGWEIVREKFPDEPNKWSRLWDTCDFERALTAYKGIK 554
Query: 545 YTEGIVLNLEKKQKELTLEAKLFADMTCLRILEI-------------------------- 604
E I L+L K K + + FA MT LR+L++
Sbjct: 555 RVETISLDL-SKLKRVCSNSNAFAKMTRLRLLKVQSSLDIDFEPEYIDADDKVELYDVVM 614
Query: 605 ---SNVQLSGNIKSLSNLLGLLNWPGYPSKCLPSNFQSGYLLELHLCRSSIVRIWEGKKE 664
S ++L K S L L W GYP LPSNF G L+ELHL S+I ++ G K+
Sbjct: 615 KNASKMRLGRGFKFPSYELRYLRWDGYPLDFLPSNFDGGKLVELHLKCSNIKQLRLGNKD 674
Query: 665 FEKLKVINVSDSEYLLETPDFSEVPNLERLVLRNCRSLYHIHPSINSLSRLIFLDLNGCS 724
E LKVI++S S L + +FS +PNLERL LR C SL IHPS+ ++ +L L L C
Sbjct: 675 LEMLKVIDLSYSRKLSQMSEFSSMPNLERLFLRGCVSLIDIHPSVGNMKKLTTLSLKSCK 734
Query: 725 SLKSFPSNL-RCKNLERLVLSKSGVTSSLEIK-GNMEHLCELRLDGTPIKQLHSSIERLV 784
LK+ P ++ ++LE L L+ K GNM+ L EL L T IK L SI L
Sbjct: 735 KLKNLPDSIGDLESLEILDLAYCSKFEKFPEKGGNMKSLTELDLQNTAIKDLPDSIGDLE 794
Query: 785 GLIVLDLRNC-----------------------IMLSSLPNEIGNLKSLESLLLGGCVNL 844
L LDL +C + LP+ I +L+SLE L L C
Sbjct: 795 SLKYLDLSDCSKFEKFPEKGGNMKSLRELDLRNTAIKDLPDSIRDLESLERLYLSYCSKF 854
Query: 845 DQIPPSLGNAQCLAYLEISETSICRLPPTIHCLKNLKGL---NCD---------GLSRSI 904
++ P GN + L L++ T+I LP +I L++LK L NC G +S+
Sbjct: 855 EKFPEKGGNMKSLMELDLQNTAIKDLPDSIGDLESLKYLDLSNCSKFEKFPEKGGNMKSL 914
Query: 905 WPSLLRPCDILGSDLGISGSKFGLESLEYLTLKGCNLVNEDIPEDLHCFSSLKTLDLSGN 946
L I + S LESL L L C+ E PE SL L L+
Sbjct: 915 TELFLENTAI----KDLPDSIGDLESLVSLNLSDCSKF-EKFPEKGGNMKSLNWLYLNNT 974
BLAST of Sgr016774 vs. ExPASy Swiss-Prot
Match:
A0A290U7C4 (Disease resistance protein Roq1 OS=Nicotiana benthamiana OX=4100 GN=ROQ1 PE=1 SV=1)
HSP 1 Score: 501.1 bits (1289), Expect = 4.5e-140
Identity = 334/955 (34.97%), Postives = 520/955 (54.45%), Query Frame = 0
Query: 15 TYDVFISFRGEDTRNNFVGFLHEALRQLGIMTFLDDKKLLIGDNLSENLVRAIEESRSSI 74
+YDVF+SFRGEDTR FVG L AL + GI TF+DDK+L G ++S L++AI ESR ++
Sbjct: 11 SYDVFLSFRGEDTRKTFVGHLFNALIEKGIHTFMDDKELKRGKSISSELMKAIGESRFAV 70
Query: 75 VVLSKDYASSKWCLKELAKIIDCMAETTHRVLPVFYHVDPSNVRHQSGSFKKSFDEHETI 134
VV SK+YASS WCL+EL KI++ + V+PVFY VDPS VR Q+G + F + E
Sbjct: 71 VVFSKNYASSTWCLEELVKILEIHEKFELIVVPVFYDVDPSTVRKQNGEYAVCFTKFEA- 130
Query: 135 AHKFIGEQKEKYLEEVQNWRDSMTKVGLLSGEVV--TEHSPEVDIVKKITNQIFERWRPK 194
+ ++ ++V WR+++TKV +SG + T + E +++I IF+++
Sbjct: 131 --NLVDDR-----DKVLRWREALTKVANISGHDLRNTYNGDESKCIQQILKDIFDKFCFS 190
Query: 195 LVTSDKNLVGMTYRLLKMNVQLGLGLDDVRFLGIVGMGGIGKTTIAKVVYDCITSKFEGS 254
+ ++++LVG+ ++ K++ L + L VR +GI GMGG+GKTT A+ +++ FE +
Sbjct: 191 ISITNRDLVGIESQIKKLSSLLRMDLKGVRLVGIWGMGGVGKTTAARALFNRYYQNFESA 250
Query: 255 CFLRVSRGSSKQIELVKLQEQLLSRLFLKENVQIWDEDYGAEMIENQLRSRKVLLVLDGV 314
CFL + + L+ LQ+ LLS+L E V D + +++ +L S+KVL+VLD V
Sbjct: 251 CFLEDVKEYLQHHTLLYLQKTLLSKLLKVEFVDCTDTEEMCVILKRRLCSKKVLVVLDDV 310
Query: 315 EEKRQVEMLAGSPDWFGPGSRVIITTRNKEVLQPYEILE-YKVDLLDSDSALQLFCNRAF 374
Q++ L G+ DWFG GSR++ITTR+ ++L+ +++ E Y++ +L+ D A++LF AF
Sbjct: 311 NHNDQLDKLVGAEDWFGSGSRIVITTRDMKLLKNHDVHETYEIKVLEKDEAIELFNLHAF 370
Query: 375 GTDYVDSNFMNLSKEIVEKLGRLPLALKVIGSYLNNKNGVIWNETLNRLIKVDEKYLFDV 434
+ F L +V+ G LPLALKV+GS L ++ +W T++RL E +
Sbjct: 371 KRSSPEKEFKELLNLVVDYTGGLPLALKVLGSLLYKEDLDVWISTIDRLKDNPEGEIMAT 430
Query: 435 LKISYDGLGAESQKVFLDLACFFNGRRIDKVIKILESFGYNPHSELHLFMERCLIEVSHS 494
LKIS+DGL + +FLD+ACFF G + + + G++P + +E+ LI +
Sbjct: 431 LKISFDGLRDYEKSIFLDIACFFRGYNQRDMTALFHASGFHPVLGVKTLVEKSLIFILED 490
Query: 495 KILMHDLILALGQEIVRKESLTQLGKQSRIWLQEDVIRAFTMKHGLKYTEGIVL----NL 554
KI MHDL+ +G++I +ES RI+ EDV A + EG++L
Sbjct: 491 KIQMHDLMQEMGRQIAVQES-----PMRRIYRPEDVKDACIGDMRKEAIEGLLLTEPEQF 550
Query: 555 EKKQKELTLEAKLFADMTCLRIL--EISNVQLSGNIKSLSNLLGLLNWPGYPSKCLPSNF 614
E+ + E A+ LRIL E N + L N L L W Y S PSNF
Sbjct: 551 EEGELEYMYSAEALKKTRRLRILVKEYYNRGFDEPVAYLPNSLLWLEWRNYSSNSFPSNF 610
Query: 615 QSGYLLELHLCRSSIVRIWEGKKEFEKLKVINVSDSEYLLETPDFSEVPNLERLVLRNCR 674
+ L+ L + SSI+ +W G K L +++S L++TPDF + NLERL+L +C
Sbjct: 611 EPSKLVYLTMKGSSIIELWNGAKRLAFLTTLDLSYCHKLIQTPDFRMITNLERLILSSCD 670
Query: 675 SLYHIHPSINSLSRLIFLDLNGCSSLKSFPSNLRCKNLERLVLSKS-GVTSSLEIKGNME 734
+L +HPS+ L LI L+++ C SL+ P+ ++ + LE L L+ + E++ NM
Sbjct: 671 ALVEVHPSVGFLKNLILLNMDHCISLERLPAIIQSECLEVLDLNYCFNLKMFPEVERNMT 730
Query: 735 HLCELRLDGTPIKQLHSSIERLVGLIVLDLRNCIMLSSLPNEIGNLKSLESLLLGGCVNL 794
HL +L L T I++L +SIE L L L + +C L SLP+ I ++L+ + C L
Sbjct: 731 HLKKLDLTSTGIRELPASIEHLSSLENLQMHSCNQLVSLPSSIWRFRNLK---ISECEKL 790
Query: 795 DQIPPSLGNAQCLAYLEISETSICRLPPTIHCLKNLKGL---NC---DGLSRSIW--PSL 854
+P GN+ C L + SI LP +I L +L L NC LS SIW SL
Sbjct: 791 GSLPEIHGNSNCTRELILKLVSIKELPTSIGNLTSLNFLEICNCKTISSLSSSIWGLTSL 850
Query: 855 ----LRPCDILGSDLGISGSKFGLESLEYLTLKGCN-LVNEDIPEDLHCFSSLKTLDLSG 914
L C L + GI ++ +L+ G L+ + P L+ +D+S
Sbjct: 851 TTLKLLDCRKLKNLPGIP------NAINHLSGHGLQLLLTLEQPTIYERLDLLRIIDMSW 910
Query: 915 NDFV-RLPESVNHLKNLRELRLHDCFKLQHLPQLPTNLVPIVSYTESGTPVRSFP 946
+ LP ++ LK LR L + C +L++LP+ +L + GT + P
Sbjct: 911 CSCISSLPHNIWMLKFLRILCISYCSRLEYLPENLGHLEHLEELLADGTGILRLP 943
BLAST of Sgr016774 vs. ExPASy Swiss-Prot
Match:
Q9SZ66 (Disease resistance-like protein DSC1 OS=Arabidopsis thaliana OX=3702 GN=DSC1 PE=1 SV=1)
HSP 1 Score: 492.3 bits (1266), Expect = 2.1e-137
Identity = 325/940 (34.57%), Postives = 500/940 (53.19%), Query Frame = 0
Query: 16 YDVFISFRGEDTRNNFVGFLHEALRQLGIMTFLDDKKLLIGDNLSENLVRAIEESRSSIV 75
+DVF+SFRG DTRNNF G L +ALR GI +F+DD +L GDNL+ L IE+S+ +I+
Sbjct: 11 FDVFLSFRGFDTRNNFTGHLQKALRLRGIDSFIDD-RLRRGDNLTA-LFDRIEKSKIAII 70
Query: 76 VLSKDYASSKWCLKELAKIIDCMAETTHRVLPVFYHVDPSNVRHQSGSFKKSFDEHETIA 135
V S +YA+S WCL+EL KI++C V+P+FY VD S+V Q SF F E
Sbjct: 71 VFSTNYANSAWCLRELVKILECRNSNQQLVVPIFYKVDKSDVEKQRNSFAVPFKLPEL-- 130
Query: 136 HKFIGEQKEKYLEEVQNWRDSMTKVGLLSGEVVTEHS-PEVDIVKKITNQIFERWRPKLV 195
F G EE+ +W+ ++ + G VV E S E +V +I F++
Sbjct: 131 -TFPGVTP----EEISSWKAALASASNILGYVVKEISTSEAKLVDEIAVDTFKKLNDLAP 190
Query: 196 TSDKNLVGMTYRLLKMNVQLGL-GLDDVRFLGIVGMGGIGKTTIAKVVYDCITSKFEGSC 255
+ ++ LVG+ RL + L LD V +GIVGM GIGKTT+A +Y + +F+GSC
Sbjct: 191 SGNEGLVGIESRLKNLEKLLSWEDLDTVHIIGIVGMVGIGKTTLADCLYGRMRGQFDGSC 250
Query: 256 FLRVSRGSSKQIELVKLQEQLLSRLFLKENVQIWDEDYGAEMIENQLRSRKVLLVLDGVE 315
FL R +S + L L ++L S + +++I E E +L+S+++L+VLD V
Sbjct: 251 FLTNIRENSGRSGLESLLQKLFSTVLNDRDLEIGAPGNAHERFERRLKSKRLLIVLDDVN 310
Query: 316 EKRQVEMLAGSPDWFGPGSRVIITTRNKEVLQPYEILEYKVDLLDSDSALQLFCNRAFGT 375
+++Q+ L G W+ GSR+IITTR+ ++++ + +Y + L+ AL+LF AF
Sbjct: 311 DEKQIRYLMGHCKWYQGGSRIIITTRDSKLIETIKGRKYVLPKLNDREALKLFSLNAFSN 370
Query: 376 DYVDSNFMNLSKEIVEKLGRLPLALKVIGSYLNNKNGVIWNETLNRLIKVDEKYLFDVLK 435
+ F L+ +++ PLALKV+GS L ++ + W L+RL +++VL+
Sbjct: 371 SFPLKEFEGLTNMVLDYAKGHPLALKVLGSDLCERDDLYWEAKLDRLKSRSHGDIYEVLE 430
Query: 436 ISYDGLGAESQKVFLDLACFFNGRRIDKVIKILESFGYNPHSELHLFMERCLIEVSHSKI 495
SY+ L E + VFLD+ACFF +D V +L S G + + +++CLI +S ++I
Sbjct: 431 TSYEELTTEQKNVFLDIACFFRSENVDYVTSLLNSHGVDVSGVVKDLVDKCLITLSDNRI 490
Query: 496 LMHDLILALGQEIVRKES---------LTQLGKQS----RIWLQEDVIRAFTMKHGLKYT 555
MHD++ + +EI K L++ G Q R+W ED+ T G
Sbjct: 491 EMHDMLQTMAKEISLKVETIGIRDCRWLSRHGNQCQWHIRLWDSEDICDLLTEGLGTDKI 550
Query: 556 EGIVLNLEKKQKELTLEAKLFADMTCLRILEISN------------VQLSGNIKSLSNLL 615
GI L+ K + + L AK F M L+ L+I + + L + L N L
Sbjct: 551 RGIFLD-TSKLRAMRLSAKAFQGMYNLKYLKIYDSHCSRGCEAEFKLHLRRGLSFLPNEL 610
Query: 616 GLLNWPGYPSKCLPSNFQSGYLLELHLCRSSIVRIWEGKKEFEKLKVINVSDSEYLLETP 675
L+W GYP + +P +F L++L L S + IW+ +K+ LK +++S S L +
Sbjct: 611 TYLHWHGYPLQSIPLDFDPKNLVDLKLPHSQLEEIWDDEKDVGMLKWVDLSHSINLRQCL 670
Query: 676 DFSEVPNLERLVLRNCRSLYHIHPSINSLSRLIFLDLNGCSSLKSFPSNLRCKNLERLVL 735
+ NLERL L C SL + +IN L +LI+L+L C+SL+S P ++ ++L+ L+L
Sbjct: 671 GLANAHNLERLNLEGCTSLKKLPSTINCLEKLIYLNLRDCTSLRSLPKGIKTQSLQTLIL 730
Query: 736 SKSGVTSSLEIKGNMEHLCELRLDGTPIKQLHSSIERLVGLIVLDLRNCIMLSSLPNEIG 795
SG +S + E++ L LDGT IK L SI+ L +L+L+NC L L +++
Sbjct: 731 --SGCSSLKKFPLISENVEVLLLDGTVIKSLPESIQTFRRLALLNLKNCKKLKHLSSDLY 790
Query: 796 NLKSLESLLLGGCVNLDQIPPSLGNAQCLAYLEISETSICRLPPTIHCLKNLKGLNCDGL 855
LK L+ L+L GC L+ P + + L L + +TSI +P +H L N+K + G
Sbjct: 791 KLKCLQELILSGCSQLEVFPEIKEDMESLEILLMDDTSITEMPKMMH-LSNIKTFSLCGT 850
Query: 856 SRSIWPSLLRPCDILGSDLGISGSKFGLESLEYLTLKGCNLVNEDIPEDLHCFSSLKTLD 915
S + S+ G L L L C+L +P+++ SSL++L
Sbjct: 851 SSHVSVSMF-----------FMPPTLGCSRLTDLYLSRCSLYK--LPDNIGGLSSLQSLC 910
Query: 916 LSGNDFVRLPESVNHLKNLRELRLHDCFKLQHLPQLPTNL 929
LSGN+ LPES N L NL+ L C L+ LP LP NL
Sbjct: 911 LSGNNIENLPESFNQLNNLKWFDLKFCKMLKSLPVLPQNL 924
BLAST of Sgr016774 vs. ExPASy TrEMBL
Match:
A0A6J1CJ31 (TMV resistance protein N-like OS=Momordica charantia OX=3673 GN=LOC111012055 PE=4 SV=1)
HSP 1 Score: 2598.5 bits (6734), Expect = 0.0e+00
Identity = 1303/1582 (82.36%), Postives = 1417/1582 (89.57%), Query Frame = 0
Query: 1 MEVPSSTPPNRPRMTYDVFISFRGEDTRNNFVGFLHEALRQLGIMTFLDDKKLLIGDNLS 60
MEV S+ PPNRPRMTYDVFISFRGEDTRNNFVGFL++ALRQLGIMTF+DDKKLLIGD+LS
Sbjct: 29 MEVSSTAPPNRPRMTYDVFISFRGEDTRNNFVGFLYQALRQLGIMTFMDDKKLLIGDDLS 88
Query: 61 ENLVRAIEESRSSIVVLSKDYASSKWCLKELAKIIDCMAETTHRVLPVFYHVDPSNVRHQ 120
E V+AIEESRSSIVVLSKDYASSKWCLKELAKI+DCM+ETTHRVLPVFYHVDPS+VRHQ
Sbjct: 89 EKFVKAIEESRSSIVVLSKDYASSKWCLKELAKIMDCMSETTHRVLPVFYHVDPSHVRHQ 148
Query: 121 SGSFKKSFDEHETIAHKFIGEQKEKYLEEVQNWRDSMTKVGLLSGEVVTEHSPEVDIVKK 180
SG+FKKSFD HE A K IG Q+E+YL+EVQNW+DSMTK+G L+GEVVT+HS EVD+VKK
Sbjct: 149 SGNFKKSFDGHEEKAPKLIGVQREEYLKEVQNWKDSMTKIGNLAGEVVTQHSSEVDVVKK 208
Query: 181 ITNQIFERWRPKLVTSDKNLVGMTYRLLKMNVQLGLGLDDVRFLGIVGMGGIGKTTIAKV 240
ITNQIFERWRPK+ TSDKNLVGMT +LL+MNV+LGLGLDDVRF+GIVGMGGIGKTTIAKV
Sbjct: 209 ITNQIFERWRPKIATSDKNLVGMTRQLLEMNVKLGLGLDDVRFVGIVGMGGIGKTTIAKV 268
Query: 241 VYDCITSKFEGSCFLRVSRGSSKQIELVKLQEQLLSRLFLKENVQIWDEDYGAEMIENQL 300
VYDCITSKFEGSCFLRV GSSKQ LV LQEQLLSRLFLKENV+IWDEDYGAEMIENQL
Sbjct: 269 VYDCITSKFEGSCFLRVFGGSSKQCNLVSLQEQLLSRLFLKENVRIWDEDYGAEMIENQL 328
Query: 301 RSRKVLLVLDGVEEKRQVEMLAGSPDWFGPGSRVIITTRNKEVL-QPYEILEYKVDLLDS 360
SRKVLLVLD VEEK Q+EMLA SPDWFGPGSR+IITTRNKE+L QPYEILEYK+ LLD
Sbjct: 329 CSRKVLLVLDEVEEKEQLEMLARSPDWFGPGSRIIITTRNKEILHQPYEILEYKMKLLDD 388
Query: 361 DSALQLFCNRAFGTDYVDSNFMNLSKEIVEKLGRLPLALKVIGSYLNNKNGVIWNETLNR 420
DSALQLFCNRAFG DY+DS+FMNLSK +VEKLGRLPLALKVIGSYLN K+ +IW ETLNR
Sbjct: 389 DSALQLFCNRAFGIDYIDSSFMNLSKGMVEKLGRLPLALKVIGSYLNKKDVLIWKETLNR 448
Query: 421 LIKVDEKYLFDVLKISYDGLGAESQKVFLDLACFFNGRRIDKVIKILESFGYNPHSELHL 480
LIKVDEK+LF+VL ISYDGLGAESQKVFLDLACFFNGRR DKVIKILESFGYNPHSEL+L
Sbjct: 449 LIKVDEKHLFEVLNISYDGLGAESQKVFLDLACFFNGRRTDKVIKILESFGYNPHSELNL 508
Query: 481 FMERCLIEVSHSKILMHDLILALGQEIVRKESLTQLGKQSRIWLQEDVIRAFTMKHGLKY 540
F E+CLIEVSHSKILMHDLI+ALGQEIVRKESLTQLGKQSRIWLQEDV+RAFTMKHGLKY
Sbjct: 509 FTEKCLIEVSHSKILMHDLIVALGQEIVRKESLTQLGKQSRIWLQEDVVRAFTMKHGLKY 568
Query: 541 TEGIVLNLEKKQKELTLEAKLFADMTCLRILEISNVQLSGNIKSLSNLLGLLNWPGYPSK 600
TEGIVLNLEKKQKELTLEAKLFADMTCLRILEISNVQL GNIKSLSNLL LLNWPGYPSK
Sbjct: 569 TEGIVLNLEKKQKELTLEAKLFADMTCLRILEISNVQLYGNIKSLSNLLALLNWPGYPSK 628
Query: 601 CLPSNFQSGYLLELHLCRSSIVRIWEGKKEFEKLKVINVSDSEYLLETPDFSEVPNLERL 660
CLPSNFQSGYLLELHL SSIVRIW+GKKEFE LKVI+VSDSEYLLETP+FS+VP LERL
Sbjct: 629 CLPSNFQSGYLLELHLRHSSIVRIWDGKKEFENLKVIDVSDSEYLLETPNFSKVPKLERL 688
Query: 661 VLRNCRSLYHIHPSINSLSRLIFLDLNGCSSLKSFPSNLRCKNLERLVLSKSGVTSSLEI 720
VL NCR L HIHPSINSL L FLDL CSSLKSF SNLRCKNLERLVLSKSGVTSS EI
Sbjct: 689 VLCNCRRLNHIHPSINSLHLLKFLDLTECSSLKSFSSNLRCKNLERLVLSKSGVTSSPEI 748
Query: 721 KGNMEHLCELRLDGTPIKQLHSSIERLVGLIVLDLRNCIMLSSLPNEIGNLKSLESLLLG 780
+ NME LCEL LDGTPIKQLH SI RLVGLI LDLR CIMLSSLPNEIGNL+SL+SLLLG
Sbjct: 749 EENMERLCELYLDGTPIKQLHPSIGRLVGLIKLDLRKCIMLSSLPNEIGNLESLQSLLLG 808
Query: 781 GCVNLDQIPPSLGNAQCLAYLEISETSICRLPPTIHCLKNLKGLNCDGLSRSIWPSLLRP 840
GC+NLDQ+PPSLGN Q L YLEI ETSIC LPPTIH LKNLKGL DGLS IW SLL P
Sbjct: 809 GCINLDQMPPSLGNVQRLQYLEICETSICHLPPTIHSLKNLKGLKFDGLSGRIWHSLLPP 868
Query: 841 CDILGSDLGISGSKFGLESLEYLTLKGCNLVNEDIPEDLHCFSSLKTLDLSGNDFVRLPE 900
+IL SDLGISGSKFG+ESLEYL+L+GCNLV EDIPEDLH FSSLKTLDLSGN+FVRLPE
Sbjct: 869 SNILESDLGISGSKFGMESLEYLSLRGCNLVEEDIPEDLHYFSSLKTLDLSGNNFVRLPE 928
Query: 901 SVNHLKNLRELRLHDCFKLQHLPQLPTNLVPIVSYTESGTPVRSFPIPYQHGKHFILEFI 960
S+NHLKNLRELRLHDCFKLQHLP+L TNLVPI+S TESG VR+ PIPY H KHFILEFI
Sbjct: 929 SINHLKNLRELRLHDCFKLQHLPELRTNLVPIISCTESGA-VRNLPIPYHHDKHFILEFI 988
Query: 961 PKTNLKLDPKAFEESILKGVLFSSIDDKAEVLECFDEIREGNLIEIEFYQIKSDANNTYG 1020
PKTNLKLDPKAFEESI +G LFSS DD AEVLECFDEIREGN+IEIE YQIK+DANN YG
Sbjct: 989 PKTNLKLDPKAFEESISEGALFSSTDDNAEVLECFDEIREGNVIEIERYQIKNDANNIYG 1048
Query: 1021 VVLSACIQCSPNTTPEEVVTFYSKFFIELEMEENPRIGILPVTSKLTSRTRFWMLFIPFH 1080
VVLSACIQCS NT EV++FY KF IEL MEE+PR GILP+TSKLTSRTRFWMLFIP +
Sbjct: 1049 VVLSACIQCSQNTA-HEVISFYCKFLIELGMEEHPRTGILPLTSKLTSRTRFWMLFIPLN 1108
Query: 1081 MLPKCRLRFKIVVKGSFPSVNVQKCGASILSHQNAPKFLANMFNMMYRTCNQNKFCTTIR 1140
+LPK RLRFKI+ KGS ++N+QKCGAS+LSHQNAP+ LANMFN +YRT NQ++FCTTIR
Sbjct: 1109 LLPKSRLRFKILAKGSPNTINIQKCGASVLSHQNAPELLANMFNKVYRTRNQDEFCTTIR 1168
Query: 1141 ECNHGSECSCDEPETVDQYCTTQYVENESDSFSLLKRNLKSILQRTYEEENHRSLVYFFP 1200
ECNHGS+CSCDEPET+DQYC+TQYVENES FSLLKR+LKSIL+RTYEEE SL YFFP
Sbjct: 1169 ECNHGSKCSCDEPETIDQYCSTQYVENESGPFSLLKRSLKSILRRTYEEEKDHSLYYFFP 1228
Query: 1201 QGQVSSWFRIQQPKDTVAVKLPLNLFREKKWMGLAVFAIFSIPGNAEFVHNHIFTCQISS 1260
+G SWFRIQQPKDTVAVKLPL+LF+EKKWMGLAVFAIFS+PGN+E+VHNH F QI+S
Sbjct: 1229 RGPALSWFRIQQPKDTVAVKLPLDLFKEKKWMGLAVFAIFSVPGNSEYVHNHTFNFQITS 1288
Query: 1261 DEDGRHLMKGGSYSSHSFLIPLSLSQFSPQILFVTFKPRHVFPYVQDGSNHFYVSFISKA 1320
+EDGRHL+KGGSYSS SFLIPLSL Q SPQILFVTF+PRHVFPYVQ+G NHFYVSFIS
Sbjct: 1289 NEDGRHLIKGGSYSSGSFLIPLSLLQSSPQILFVTFEPRHVFPYVQEGLNHFYVSFISIP 1348
Query: 1321 TRLRVESCGARLVYQHNVEGLIHTIMDCVLKSGQELFQEYSGKILSDALTTYERLIWIRV 1380
TRL+VESCGARLVYQHNVEGLI+TIMDCV KS +EL+Q++S +I+ D LTTY R I I+V
Sbjct: 1349 TRLQVESCGARLVYQHNVEGLINTIMDCVPKSREELYQDHSQEIMRDFLTTYHRPIRIQV 1408
Query: 1381 PADEPTTSTSSDLSGHHSTNKLLHENFQRPLFKESTQDRYK-SKFDAIIHGKEIPKFFTS 1440
PAD+ TTSTSSDLSG E+FQ LF ESTQD+Y SK +AIIHGKEIP FFTS
Sbjct: 1409 PADQLTTSTSSDLSGR-------RESFQHSLFMESTQDQYNISKLEAIIHGKEIPDFFTS 1468
Query: 1441 QNMGNEGTVRIPERLYFAKCEVGVLVCAVVAINKQREKLERISELQSCVKVLDLKCEFGV 1500
Q+MGN+G VR PERLYFAKCEVGVLVCAVV IN Q EKLE VKVLDLKCEFGV
Sbjct: 1469 QSMGNKGAVRTPERLYFAKCEVGVLVCAVVVINNQTEKLEH-------VKVLDLKCEFGV 1528
Query: 1501 DSHEVEPEHHLFLPAEALSSQYEPSFIWLSCIPLAEFNVPWMCCDYLKSKVSSNHEELFH 1560
DSHE+EPEHHLF+PAE L S +E SFIWLSCIPL EFN+PWMCCDYL+ KVS+NHEELFH
Sbjct: 1529 DSHEIEPEHHLFVPAEKLLSSFESSFIWLSCIPLTEFNIPWMCCDYLRCKVSTNHEELFH 1588
Query: 1561 VQQCGLHLLYVSERKLVDNLLM 1581
VQQCGLHLLY SERK+VDNLL+
Sbjct: 1589 VQQCGLHLLYASERKMVDNLLI 1594
BLAST of Sgr016774 vs. ExPASy TrEMBL
Match:
A0A6J1CL15 (TMV resistance protein N-like OS=Momordica charantia OX=3673 GN=LOC111012122 PE=4 SV=1)
HSP 1 Score: 2206.8 bits (5717), Expect = 0.0e+00
Identity = 1129/1589 (71.05%), Postives = 1294/1589 (81.43%), Query Frame = 0
Query: 1 MEVPSSTPPNRPRMTYDVFISFRGEDTRNNFVGFLHEALRQLGIMTFLDDKKLLIGDNLS 60
MEVPS+ PPNRPRMTYDVFISFRGEDTRNNFVGFL+EAL QLGIMTF+DD+KLLIGD+LS
Sbjct: 44 MEVPSTAPPNRPRMTYDVFISFRGEDTRNNFVGFLYEALHQLGIMTFMDDRKLLIGDDLS 103
Query: 61 ENLVRAIEESRSSIVVLSKDYASSKWCLKELAKIIDCMAETTHRVLPVFYHVDPSNVRHQ 120
ENL++AIEESRSSIVVLSKDYASSKWCLKEL KI+DCM+ETTH+VLPVFYHVDPS+VRHQ
Sbjct: 104 ENLMKAIEESRSSIVVLSKDYASSKWCLKELVKIMDCMSETTHQVLPVFYHVDPSHVRHQ 163
Query: 121 SGSFKKSFDEHETIAHKFIGEQKEKYLEEVQNWRDSMTKVGLLSGEVVTEHSPEVDIVKK 180
SG+FKKSFDEHE A K G+QKEKYL EV+NW+DSMTK+G L+GEVVT+HS EV+IVKK
Sbjct: 164 SGNFKKSFDEHEEDAKKITGQQKEKYLVEVKNWKDSMTKIGNLTGEVVTQHSSEVNIVKK 223
Query: 181 ITNQIFERWRPKLVTSDKNLVGMTYRLLKMNVQLGLGLDDVRFLGIVGMGGIGKTTIAKV 240
ITNQIFERWRPK+ TSDKNLVGMTY+LLKMNV+LGLGLDDVRF+GIVGMGGIGKTTIAKV
Sbjct: 224 ITNQIFERWRPKIATSDKNLVGMTYQLLKMNVKLGLGLDDVRFVGIVGMGGIGKTTIAKV 283
Query: 241 VYDCITSKFEGSCFLRVSRGSSKQIELVKLQEQLLSRLFLKENVQIWDEDYGAEMIENQL 300
VYDCI S FEGSCFLRV GSSKQ L+ LQEQLLSRLFLKENV++WDEDYGAEMI+NQL
Sbjct: 284 VYDCIKSNFEGSCFLRVFGGSSKQNNLLSLQEQLLSRLFLKENVRMWDEDYGAEMIKNQL 343
Query: 301 RSRKVLLVLDGVEEKRQVEMLAGSPDWFGPGSRVIITTRNKEVL-QPYEILEYKVDLLDS 360
SRKVLLVLDGVEEK Q+EMLA SPDWFGPGSR+IITTRNKE+L QPYEI+EYK+ LLD+
Sbjct: 344 CSRKVLLVLDGVEEKEQLEMLARSPDWFGPGSRIIITTRNKEILHQPYEIIEYKLKLLDA 403
Query: 361 DSALQLFCNRAFGTDYVDSNFMNLSKEIVEKLGRLPLALKVIGSYLNNKNGVIWNETLNR 420
DSALQLFCNRAFG DY+DSNFMNLSKEI+EKLGRLPLALKVIGSYLN K VIW ETL R
Sbjct: 404 DSALQLFCNRAFGIDYIDSNFMNLSKEILEKLGRLPLALKVIGSYLNKKEEVIWKETLYR 463
Query: 421 LIKVDEKYLFDVLKISYDGLGAESQKVFLDLACFFNGRRIDKVIKILESFGYNPHSELHL 480
LIKVDEK LF+VLKISYDGLG ESQKVFLDLAC F+GR+ DKVI+ILE FGY+P+SEL L
Sbjct: 464 LIKVDEKNLFEVLKISYDGLGVESQKVFLDLACLFHGRKKDKVIEILECFGYSPYSELQL 523
Query: 481 FMERCLIEVSHSKILMHDLILALGQEIVRKESLTQLGKQSRIWLQEDVIRAFTMKHGLKY 540
FMERCL+EVS +KILMHDL++ALGQEIV +ESLTQ GKQSRIW QEDV R FT+KHGLKY
Sbjct: 524 FMERCLLEVSPTKILMHDLVVALGQEIVHRESLTQPGKQSRIWFQEDVDRVFTVKHGLKY 583
Query: 541 TEGIVLNLEKKQKELTLEAKLFADMTCLRILEISNVQLSGNIKSLSNLLGLLNWPGYPSK 600
TEGIVLNLEKKQKEL LEAK F DMT LRILEISNVQLSGNI LSNLLG +NWPGYPSK
Sbjct: 584 TEGIVLNLEKKQKELILEAKSFTDMTYLRILEISNVQLSGNINFLSNLLGFINWPGYPSK 643
Query: 601 CLPSNFQSGYLLELHLCRSSIVRIWEGKKEFEKLKVINVSDSEYLLETPDFSEVPNLERL 660
CLP NFQS LLELHL SSI +WEGKK+FEKLKVI+VSDS+YLL TPDFS+VPNLERL
Sbjct: 644 CLPPNFQSRCLLELHLPLSSIEILWEGKKDFEKLKVIDVSDSKYLLGTPDFSKVPNLERL 703
Query: 661 VLRNCRSLYHIHPSINSLSRLIFLDLNGCSSLKSFPSNLRCKNLERLVLSKSGVTSSLEI 720
VLRNCR LYHIHPSIN L+RL LDL+ CSS KSF S+LRCK+LE L+LS+SG+TSS +
Sbjct: 704 VLRNCRKLYHIHPSINDLNRLRLLDLSNCSSFKSFSSHLRCKSLEGLILSESGLTSSPKF 763
Query: 721 KGNMEHLCELRLDGTPIKQLHSSIERLVGLIVLDLRNCIMLSSLPNEIGNLKSLESLLLG 780
+ NMEHL L L GTPIKQLHSSI L+GL++LDLRNCIMLSSLP+EIGNLKSL+ LLL
Sbjct: 764 EVNMEHLSRLHLVGTPIKQLHSSIGCLIGLVLLDLRNCIMLSSLPSEIGNLKSLQVLLLE 823
Query: 781 GCVNLDQIPPSLGNAQCLAYLEISETSICRLPPTIHCLKNLKGLNCDGLSRSIWPSLLRP 840
GC +LDQIPPSLGNAQCL YL+I +TSIC+ PPTIH L+NLK LNC+GLSR IW SLL P
Sbjct: 824 GCKSLDQIPPSLGNAQCLEYLDIGKTSICQAPPTIHYLENLKRLNCEGLSRKIWHSLL-P 883
Query: 841 CDILGSDLGISGSKFGLESLEYLTLKGCNLVNEDIPEDLHCFSSLKTLDLSGNDFVRLPE 900
DI S+ ISG FGL+SLE+L LK CNLV+EDIPEDLHCFSSL++LDLSGN FV+ P+
Sbjct: 884 RDIQRSNRQISGLGFGLKSLEHLCLKNCNLVDEDIPEDLHCFSSLESLDLSGNHFVQYPK 943
Query: 901 SVNHLKNLRELRLHDCFKLQHLPQLPTNLVPIVSYTESGTPVRSFPIPYQHGKHFILEFI 960
S+NHL NL++L HDCFKLQ +P+ NL P S ESG P+ +P Q K F LE+
Sbjct: 944 SINHLVNLKQLMFHDCFKLQQIPKPSINLEPKFSSVESGMPIDQ-SLPCQLDKPFRLEYG 1003
Query: 961 PKTNLKLDPKAFEESILKGVLFSSIDDKAEVLECFDEIREGNLIEIEFYQIKSDANNTYG 1020
+ KLDPK +EE IL+ F + DKAE+LECFDEIREGN +E+E+ Q+K+DAN YG
Sbjct: 1004 SEIKSKLDPKTYEELILERATFGTTCDKAEILECFDEIREGNSVEVEYNQVKNDANIIYG 1063
Query: 1021 VVLSACIQCSPNTTPEEVVTFYSKFFIELEMEENPRIGILPVTSKLTSRTRFWMLFIPFH 1080
VVLSA IQCS NT+ EV TFY F IELE + NP I + T LTSR RFW LFIP H
Sbjct: 1064 VVLSASIQCSKNTSHNEVDTFYCNFSIELETDMNPTITVFYGTFALTSRIRFWTLFIPSH 1123
Query: 1081 MLPKCR------LRFKIVVKGSFPSVNVQKCGASILSHQNAPKFLANMFNMMYRTCNQNK 1140
LPK FK +K SV++Q CGASILSHQNA +F+A MF+ MY C Q K
Sbjct: 1124 KLPKSTSMQNSLCSFKATIKYEVSSVHIQMCGASILSHQNASQFMAKMFSRMY--CIQEK 1183
Query: 1141 FCTTIRECNHGSECSCDEPETVDQYCTTQYVENESDSFSLLKRNLKSILQRTYEEENHRS 1200
F T I NH SEC+CDEPETV+ Q+VENE DSFSLL+RNLKSIL+RTYE+ H
Sbjct: 1184 FQTFIVGGNHWSECNCDEPETVEHNYLIQFVENERDSFSLLRRNLKSILRRTYEDTKHIF 1243
Query: 1201 LVYFFPQGQVSSWFRIQQPKDTVAVKLPLNLFREKKWMGLAVFAIFSIPGNAEFVHNHIF 1260
YFFPQ QV SWFRIQQ DTV +KLP N+FREKKWMGLA+FA+FS+ N+EFV +H F
Sbjct: 1244 PFYFFPQNQVPSWFRIQQLNDTVKMKLPPNMFREKKWMGLAMFAVFSVERNSEFVKDHPF 1303
Query: 1261 TCQISSDEDGRHLMKGGSYSSHS-FLIPLSLSQFSPQILFVTFKPRHVFPYVQDGSNHFY 1320
+ QI DE+G +L + SY+ HS +IPL L S +ILF+ FKPR PYVQD SNH Y
Sbjct: 1304 SWQIDIDENGTYLKRVRSYAFHSPHMIPLQLLWSSSKILFMIFKPRETSPYVQDKSNHLY 1363
Query: 1321 VSFISKATRLRVESCGARLVYQHNVEGLIHTIMDCVLKSGQELFQEYSGKILSDALTTYE 1380
V F++ +T L+VE CGARLVYQHNV+G IH I+DCVLKS Q+ Q YS KIL D L TY+
Sbjct: 1364 VKFLTNSTSLKVELCGARLVYQHNVDGFIHKIVDCVLKSEQKQCQNYSHKILRDLLATYQ 1423
Query: 1381 RLIWIRVPADEPTTSTSSDLSGHHSTNKLLHENFQRPLFKESTQDRYKSKFDAIIHGKEI 1440
+ IW +V TSTS++ + H ST L HE+ QRP FKE QDRYKSKF++IIHGKEI
Sbjct: 1424 QPIWKKV-----ATSTSNNPTQHLSTETLRHESSQRPPFKERIQDRYKSKFNSIIHGKEI 1483
Query: 1441 PKFFTSQNMGNEGTVRIPERLYFAKCEVGVLVCAVVAINKQREKLERISELQSCVKVLDL 1500
P FF S++MGNE T+RIPE + F + VGVL+CAVV I+K EKLERI E QS +KVLDL
Sbjct: 1484 PNFFISRSMGNEATIRIPEIVNFIEYHVGVLMCAVVTISKLIEKLERIPEPQSGIKVLDL 1543
Query: 1501 KCEFGVDSHEVEPEHHLFLPAEALSSQYEPSFIWLSCIPLAEFNVPWMCCDYLKSKVSSN 1560
KCEFGVDSHEV+P+HHL +PA+ L SQ SFIWL IPL+ F++P C+Y +S VSSN
Sbjct: 1544 KCEFGVDSHEVKPKHHLLVPAKVLLSQCRTSFIWLFFIPLSAFHIPLKYCNYFRSMVSSN 1603
Query: 1561 HEELFHVQQCGLHLLYVSERKLVDNLLMT 1582
+EEL VQQCGLHLL+ ERK+VDN L T
Sbjct: 1604 YEELLDVQQCGLHLLHARERKMVDNFLTT 1623
BLAST of Sgr016774 vs. ExPASy TrEMBL
Match:
A0A6J1CK95 (LOW QUALITY PROTEIN: TMV resistance protein N-like OS=Momordica charantia OX=3673 GN=LOC111012057 PE=4 SV=1)
HSP 1 Score: 2114.0 bits (5476), Expect = 0.0e+00
Identity = 1081/1403 (77.05%), Postives = 1184/1403 (84.39%), Query Frame = 0
Query: 1 MEVPSSTPPNRPRMTYDVFISFRGEDTRNNFVGFLHEALRQLGIMTFLDDKKLLIGDNLS 60
MEVPS+ PPNRPRMTYDVFISFRGEDTRNNFVGFL+EAL QLGIMTF+DDKKLLIGD+LS
Sbjct: 1 MEVPSTAPPNRPRMTYDVFISFRGEDTRNNFVGFLYEALCQLGIMTFMDDKKLLIGDDLS 60
Query: 61 ENLVRAIEESRSSIVVLSKDYASSKWCLKELAKIIDCMAETTHRVLPVFYHVDPSNVRHQ 120
E LV+AIEESRSSIVVLSKDYASSKWCLKELAKI+DCM++TTHRVLPVFYHVDPS+VRHQ
Sbjct: 61 EKLVKAIEESRSSIVVLSKDYASSKWCLKELAKIMDCMSDTTHRVLPVFYHVDPSHVRHQ 120
Query: 121 SGSFKKSFDEHETIAHKFIGEQKEKYLEEVQNWRDSMTKVGLLSGEVVTEHSPEVDIVKK 180
SG+F+KSFDEHE A KF G+QKE+Y +EV+NW+D P V
Sbjct: 121 SGNFRKSFDEHEEKAQKFTGQQKEEYWKEVRNWKD-----------------PRV----- 180
Query: 181 ITNQIFERWRPKLVTSDKNLVGMTYRLLKMNVQLGLGLDDVRFLGIVGMGGIGKTTIAKV 240
R KMN+QLGLGLDDVRF+GIVGMGGIGKT IA+V
Sbjct: 181 -------------------------RYKKMNMQLGLGLDDVRFIGIVGMGGIGKTKIAEV 240
Query: 241 VYDCITSKFEGSCFLRVSRGSSKQIELVKLQEQLLSRLFLKENVQIWDEDYGAEMIENQL 300
VYDCI SKFE +CFLRV GSS+QI LV LQEQLLSRLFLKENV+IWDEDYGAEMIE QL
Sbjct: 241 VYDCIKSKFECNCFLRVFGGSSRQINLVSLQEQLLSRLFLKENVRIWDEDYGAEMIEKQL 300
Query: 301 RSRKVLLVLDGVEEKRQVEMLAGSPDWFGPGSRVIITTRNKEVL-QPYEILEYKVDLLDS 360
SRKVLLVLDGVEEK Q+EMLA SPDWFGPGSR+IITTRNKEVL QPYEILE+KV LLD+
Sbjct: 301 CSRKVLLVLDGVEEKEQLEMLARSPDWFGPGSRIIITTRNKEVLHQPYEILEHKVKLLDN 360
Query: 361 DSALQLFCNRAFGTDYVDSNFMNLSKEIVEKLGRLPLALKVIGSYLNNKNGVIWNETLNR 420
D+ALQLFCN AFG ++D+NFMNLSKE+VE RLPLALKVIGSYLN KNGV+W ETLNR
Sbjct: 361 DNALQLFCNHAFGIAHIDNNFMNLSKEMVEXAWRLPLALKVIGSYLNKKNGVVWKETLNR 420
Query: 421 LIKVDEKYLFDVLKISYDGLGAESQKVFLDLACFFNGRRIDKVIKILESFGYNPHSELHL 480
LIKV E+ LF+VLKISYDGLG ESQKVFLDLACFFNGRR+DKVI+ILESFGYNPHSELHL
Sbjct: 421 LIKVKEERLFEVLKISYDGLGVESQKVFLDLACFFNGRRLDKVIEILESFGYNPHSELHL 480
Query: 481 FMERCLIEVSHSKILMHDLILALGQEIVRKESLTQLGKQSRIWLQEDVIRAFTMKHGLKY 540
F+E+CLIE SHSKILMHDLI+ALGQEIVRKESLTQLGKQSRIWLQEDV+RAFT+KH
Sbjct: 481 FIEKCLIEDSHSKILMHDLIVALGQEIVRKESLTQLGKQSRIWLQEDVVRAFTIKH---- 540
Query: 541 TEGIVLNLEKKQKELTLEAKLFADMTCLRILEISNVQLSGNIKSLSNLLGLLNWPGYPSK 600
AKLFADMTCLRILEISNVQLSGNIKSLSNLLGLLNWPGYPSK
Sbjct: 541 ------------------AKLFADMTCLRILEISNVQLSGNIKSLSNLLGLLNWPGYPSK 600
Query: 601 CLPSNFQSGYLLELHLCRSSIVRIWEGKKEFEKLKVINVSDSEYLLETPDFSEVPNLERL 660
CLPSNFQSGYL ELHL S I+RIW+GKKEFE LKVI+VS+SEYLLETPDFSEVP LERL
Sbjct: 601 CLPSNFQSGYLHELHLRHSGILRIWDGKKEFENLKVIDVSNSEYLLETPDFSEVPKLERL 660
Query: 661 VLRNCRSLYHIHPSINSLSRLIFLDLNGCSSLKSFPSNLRCKNLERLVLSKSGVTSSLEI 720
VLRNC LYHIH SINSL LI LDL CSS KSF +NL CKNLE LVLSKSGVTSS E
Sbjct: 661 VLRNCSRLYHIHHSINSLHHLILLDLTNCSSFKSFSTNLGCKNLEHLVLSKSGVTSSPEF 720
Query: 721 KGNMEHLCELRLDGTPIKQLHSSIERLVGLIVLDLRNCIMLSSLPNEIGNLKSLESLLLG 780
+GNMEHL +L LD TPIKQLHSSIE LVGLI+LDLRNCIMLSSLP+EIGNL SLE+L LG
Sbjct: 721 EGNMEHLRKLHLDETPIKQLHSSIEHLVGLILLDLRNCIMLSSLPDEIGNLNSLEALFLG 780
Query: 781 GCVNLDQIPPSLGNAQCLAYLEISETSICRLPPTIHCLKNLKGLNCDGLSRSIWPSLLRP 840
GCVNLDQIPPSLGNAQCL +L+I +TSICR+PPTIHCLKNL+GLNCDGLS SIW SLL P
Sbjct: 781 GCVNLDQIPPSLGNAQCLQFLDIGKTSICRVPPTIHCLKNLEGLNCDGLSCSIWRSLLPP 840
Query: 841 CDILGSDLGISGSKFGLESLEYLTLKGCNLVNEDIPEDLHCFSSLKTLDLSGNDFVRLPE 900
+ILGSDLGI KFGLESLE+L+LK CNLVNEDIPEDLH FSSLKTLDLSGN+FV LPE
Sbjct: 841 DNILGSDLGIPSLKFGLESLEFLSLKDCNLVNEDIPEDLHYFSSLKTLDLSGNNFVWLPE 900
Query: 901 SVNHLKNLRELRLHDCFKLQHLPQLPTNLVPIVSYTESGTPVRSFPIPYQHGKHFILEFI 960
S+NHLKNLREL+LHDCFKLQ LPQLPT+L PI+SYTESG +R+ PIPY H KHFILEFI
Sbjct: 901 SINHLKNLRELKLHDCFKLQQLPQLPTHLEPIISYTESGA-IRNLPIPYHHDKHFILEFI 960
Query: 961 PKTNLKLDPKAFEESILKGVLFSSIDDKAEVLECFDEIREGNLIEIEFYQIKSDANNTYG 1020
PKTNLKLD KAFEESI +G LF S DDKAEVLECFDE REGN+IEI+ YQIK+DAN+ YG
Sbjct: 961 PKTNLKLDLKAFEESISEGALFYSTDDKAEVLECFDETREGNVIEIDQYQIKNDANSIYG 1020
Query: 1021 VVLSACIQCSPNTTPEEVVTFYSKFFIELEMEENPRIGILPVTSKLTSRTRFWMLFIPFH 1080
VVL+ACIQCS N TP EV+TFY KF +ELEME NP+ LP+TSKLTS+TRFWM FIP H
Sbjct: 1021 VVLAACIQCSQN-TPHEVITFYRKFLVELEMEGNPKTLSLPLTSKLTSKTRFWMSFIPSH 1080
Query: 1081 MLPKCRLRFKIVVKGSFPS-VNVQKCGASILSHQNAPKFLANMFNMMYRTCNQNKFCTTI 1140
MLP+CR+ FKIVVKGS PS VNVQKCGA +LSHQNA +FLA MFN +Y T QNKF TTI
Sbjct: 1081 MLPECRVCFKIVVKGSLPSVVNVQKCGAFVLSHQNASRFLAYMFNKVYHTRKQNKFSTTI 1140
Query: 1141 RECNHGSECSCDEPETVDQYCTTQYVENESDSFSLLKRNLKSILQRTYEEENHRSLVYFF 1200
RECNHGS+CSCDEPET+ QYC+TQYVENESDS SLLKR+LKSILQRTYEEE SL YFF
Sbjct: 1141 RECNHGSKCSCDEPETIYQYCSTQYVENESDSLSLLKRSLKSILQRTYEEEKDHSLYYFF 1200
Query: 1201 PQGQVSSWFRIQQPKDTVAVKLPLNLFREKKWMGLAVFAIFSIPGNAEFVHNHIFTCQIS 1260
PQG SWFRIQQPKD VAVKLPL+LFREKKWMGLAVFAIFSIPGN+EFVH+H F QI+
Sbjct: 1201 PQGPAVSWFRIQQPKDRVAVKLPLDLFREKKWMGLAVFAIFSIPGNSEFVHHHTFNFQIT 1260
Query: 1261 SDEDGRHLMKGGSYSSHSFLIPLSLSQFSPQILFVTFKPRHVFPYVQDGSNHFYVSFISK 1320
S+E+ RHLM GGSY S SFLIPLSL Q SPQILFVTF PRHVFPYVQ G +HFYVSFIS
Sbjct: 1261 SNENDRHLMSGGSYLSDSFLIPLSLLQTSPQILFVTFAPRHVFPYVQTGLDHFYVSFISI 1320
Query: 1321 ATRLRVESCGARLVYQHNVEGLIHTIMDCVLKSGQELFQEYSGKILSDALTTYERLIWIR 1380
TRLRVESCGARLVYQHNVEGLI+TIMDCV+KSGQE +Q Y+ IL + LTTY R I IR
Sbjct: 1321 PTRLRVESCGARLVYQHNVEGLINTIMDCVIKSGQECYQNYNQTILRNFLTTYHRPIRIR 1332
Query: 1381 VPADEPT--TSTSSDLSGHHSTN 1400
VPA+ T TSTS++LSG H ++
Sbjct: 1381 VPANPSTTSTSTSTNLSGEHKSS 1332
BLAST of Sgr016774 vs. ExPASy TrEMBL
Match:
A0A5A7T7V5 (TMV resistance protein N-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G001220 PE=4 SV=1)
HSP 1 Score: 1431.4 bits (3704), Expect = 0.0e+00
Identity = 808/1651 (48.94%), Postives = 1090/1651 (66.02%), Query Frame = 0
Query: 1 MEVPSSTPPNRPRMTYDVFISFRGEDTRNNFVGFLHEALRQLGIMTFLDDKKLLIGDNLS 60
M+ SS+ +RP+M YDVFISFRG D R+ F G+L++AL +LGI FLD+K+ LIGD+L
Sbjct: 1 MQSSSSSSLDRPKMNYDVFISFRGRDVRHTFAGYLYDALNRLGIKAFLDNKRFLIGDDL- 60
Query: 61 ENLVRAIEESRSSIVVLSKDYASSKWCLKELAKIIDCMAETTHRVLPVFYHVDPSNVRHQ 120
+L + I+ESRS+IVVLS+DYAS+KWCL+EL KI+D M + RVLPVFYH+DPS V+ Q
Sbjct: 61 HDLFKIIDESRSAIVVLSEDYASAKWCLRELTKIMDSMGTSMERVLPVFYHIDPSIVKDQ 120
Query: 121 SGSFKKSFDEHETIAHKFI-GEQKEKYLEEVQNWRDSMTKVGLLSGEVVTEHSPEVDIVK 180
SG+FK SFDEHE A K I ++KEK L+E+QNW++++ K+G +G V+T++S EVDIV
Sbjct: 121 SGTFKTSFDEHEANALKEIDNQEKEKRLKELQNWKNALKKIGNHTGVVITKNSSEVDIVN 180
Query: 181 KITNQIFERWRPKLVTSDKNLVGMTYRLLKMNVQLGLGLDDVRFLGIVGMGGIGKTTIAK 240
KI +QIF+ WRPKL +KNLVGMT RLL MN+ LGLGLDDVRF+ IVGMGGIGKTTIA+
Sbjct: 181 KIASQIFDAWRPKLEALNKNLVGMTSRLLHMNMHLGLGLDDVRFVAIVGMGGIGKTTIAQ 240
Query: 241 VVYDCITSKFEGSCFLRVSRGSSKQIELVKLQEQLLSRLFLKENVQIWDEDYGAEMIENQ 300
VV+DCI SKF+ CFL + G SKQ LV LQ ++LS++F KE+ +IW E++G EMI+N+
Sbjct: 241 VVFDCILSKFDDCCFLTLPGGDSKQ-SLVSLQREMLSQIFHKEDFRIWHENHGVEMIKNR 300
Query: 301 LRSRKVLLVLDGVEEKRQVEMLAGSPDWFGPGSRVIITTRNKEVL---QPYEILEYKVDL 360
L RKVL+VLDGVEE+RQ+EMLAGS DWFGPGSR+IITTRNK +L E+ EY V+
Sbjct: 301 LSGRKVLIVLDGVEERRQLEMLAGSTDWFGPGSRIIITTRNKGLLCHPNYDEMKEYNVEE 360
Query: 361 LDSDSALQLFCNRAFGTDYVD-SNFMNLSKEIVEKLGRLPLALKVIGSYLNNKNGVIWNE 420
LD DSALQLF AFG+++ + +FM+LS EIVEK RLPLAL+VIGS L K IW E
Sbjct: 361 LDHDSALQLFLKHAFGSNHQNKDSFMDLSNEIVEKAKRLPLALRVIGSSLYGKEITIWRE 420
Query: 421 TLNRLIKVDEKYLFDVLKISYDGLGAESQKVFLDLACFFNGRRIDKVIKILESFGYNPHS 480
TL RLIKVDE+ FD+LKISYDGLG ESQ+VFLD+ CFFNG+ D+V +ILESFGY+P+S
Sbjct: 421 TLKRLIKVDERNFFDILKISYDGLGVESQQVFLDITCFFNGKNEDRVNEILESFGYSPNS 480
Query: 481 ELHLFMERCLIEVSHSKILMHDLILALGQEIVRKESLTQLGKQSRIWLQEDVIRAFTMKH 540
EL L M+RCLIEVSH KIL+HDLIL +G+EIVRKESLTQ KQSRIWL ED+ F KH
Sbjct: 481 ELQLLMQRCLIEVSHKKILVHDLILEMGREIVRKESLTQPEKQSRIWLHEDLYCRFAEKH 540
Query: 541 GLKYTEGIVLNLEKKQKE-LTLEAKLFADMTCLRILEISNVQLSGNIKSLSNLLGLLNWP 600
L + +GIVL+LEK+ +E + L+A+ F++MT LRILEI+NV+L +I+ LS LL ++NW
Sbjct: 541 DLMHIQGIVLSLEKEMEESIELDAESFSEMTKLRILEINNVELDEDIEYLSPLLRIINWL 600
Query: 601 GYPSKCLPSNFQSGYLLELHLCRSSIVRIWEGKKEFEKLKVINVSDSEYLLETPDFSEVP 660
GYPSK LP FQS YL EL L S ++R+W+GKK F KLK+I+VS+SE+L TPDFS VP
Sbjct: 601 GYPSKSLPPTFQSRYLFELLLPHSHLLRVWDGKKRFPKLKLIDVSNSEHLRVTPDFSGVP 660
Query: 661 NLERLVLRNCRSLYHIHPSINSLSRLIFLDLNGCSSLKSFPSNLRCKNLERLVLSKSGVT 720
NLERLVL NC L IHPSINSL++LI LDL GC LK FP+N+RCKNL+ L LS +G+
Sbjct: 661 NLERLVLCNCVRLCEIHPSINSLNKLILLDLEGCGDLKHFPANIRCKNLQTLKLSGTGLE 720
Query: 721 SSLEIKGNMEHLCELRLDGTPIKQLHSSIERLVGLIVLDLRNCIMLSSLPNEIGNLKSLE 780
EI G+MEHL L LDG+ I LH SI L GL+ LDL C+ LSSLP EIGNLKSL+
Sbjct: 721 IFPEI-GHMEHLTHLHLDGSKITHLHPSIGYLTGLVFLDLSTCLGLSSLPFEIGNLKSLK 780
Query: 781 SLLLGGCVNLDQIPPSLGNAQCLAYLEISETSICRLPPT-IHCLKNLKGLNCDGLSRSIW 840
+LLL C LD+IPPSL NA+ L L ISETSI +P + IHCLKNL+ L+C+GLSR IW
Sbjct: 781 TLLLKYCKRLDKIPPSLANAESLETLSISETSITHVPSSIIHCLKNLETLDCEGLSRGIW 840
Query: 841 PSLLRPCDILGSDLGISGSKFGLESLEYLTLKGCNLVNEDIPEDLHCFSSLKTLDLSGND 900
SLL +I + GL L+ L L GC L++EDIPEDLHCFSSL+ LDLS N+
Sbjct: 841 KSLLPQLNINQT------ITTGLGCLKALNLMGCKLMDEDIPEDLHCFSSLEALDLSYNN 900
Query: 901 FVRLPESVNHLKNLRELRLHDCFKLQHLPQLPTNLVPIVSYTESGTPVRSFP-------- 960
F LP+S++HLK L+ L L+ C +L+ LP+LP +L + G RS
Sbjct: 901 FTTLPDSLSHLKKLKTLNLNYCTELKDLPKLPESLQYV-----GGVDCRSMSEQYYNKIL 960
Query: 961 -IPYQHGKHFILEF-IPKTNLKLD------------PKAFEESILKGVLFSSIDDKAEVL 1020
IP G L F IP + ++ ++FE+SI++ + + D ++
Sbjct: 961 LIPSSSGHQLYLTFIIPSKDADVECAMNEFQHSIFTRRSFEQSIIEEQPSTIVHDTVDMF 1020
Query: 1021 ECFDEIREGNLIEIEFYQIKS------------DANNTYGVVLSACIQCSPNTTPEEVVT 1080
+ F +I EGN I++ Q S D +N GV LS I+ N +
Sbjct: 1021 QWFGQINEGNWTNIQYEQEFSISKPLNIMYEDVDLSNVCGVFLSTNIEFPQNLNHLAIGR 1080
Query: 1081 FYSKFFIELEMEENPRIGILPVTSKLTSRTRFWMLFIPFHMLPKCRLRFK----IVVKGS 1140
F F I+ + + S+ + FW+ +IP M+ L + + V S
Sbjct: 1081 FLVSFGIDGKCSGGT---MNYEMSQFKAARFFWVAYIPIWMIKDHSLMVQRCCSVKVTIS 1140
Query: 1141 F-------PSVNVQKCG-ASILSHQNAPKFLANMFNMMYRTCNQNKFCTTIRECN-HGSE 1200
+ V ++ CG +S+LS N ++LA +F R C++ F T IR+ N H +E
Sbjct: 1141 YCCDHIDASKVKIKACGVSSMLSWPNVAEYLAKLFT--ERFCSKRNFYTMIRQHNDHQTE 1200
Query: 1201 CSCDEPET-VDQYCTTQYVENESDSFSLLKRNLKSILQRTYEEENHRSLVYFFPQGQVSS 1260
C CDE E D + ++ + N DS LL++NL++IL +EE+ + YFFP ++
Sbjct: 1201 CRCDELEVGKDDFSSSTFESN--DSTFLLRKNLRAILGVMFEEKKRYYMKYFFPHTKIFG 1260
Query: 1261 WFRIQQPKDTVAVKLPLNLFREKKWMGLAVFAIFSIPGNAEFVHNHIFTCQISSDED--- 1320
WF+ Q KD VAVK+P+N+ +++KWMGLA+F +FSI +E + F +I + E+
Sbjct: 1261 WFKNQNKKDKVAVKIPVNIEKDRKWMGLAMFVVFSI---SEKASCYCFEYEIQTKENIIS 1320
Query: 1321 -GRHLMKGGSYSSHSFLIPLSLSQFSPQILFVTFKPRH-VFPYVQ---DGSNHFYVSFIS 1380
RH + H S QILFV F+PR+ +PY + SNH Y++F +
Sbjct: 1321 TQRHFISTDQVLEH-----------SNQILFVAFEPRYNWYPYDELKSSSSNHVYINFNT 1380
Query: 1381 KATRLRVESCGARLVYQHNVEGLIHTIMDCVLKSGQELFQEYSGKILSDALTTYERLIWI 1440
R+RVE CGARLVYQ NVEGLIHTI++C+ +SG EL++ Y+ I+ L T+ W
Sbjct: 1381 NGARMRVEFCGARLVYQQNVEGLIHTILNCIAESGDELYEHYNQYIVESHL-TFINTHWY 1440
Query: 1441 RVP-------ADEPTTSTSSDLSGHHSTNKLLHENFQRPLFKESTQDRYKSKFDAIIHGK 1500
+ ++P+T+ S+ + S LL+ +F P F +S Q+R++SKFD ++HG
Sbjct: 1441 TLSFRRNNSVKNQPSTAASTCTASSLSVEHLLYGSFPHPFFHKSLQERFRSKFDLLLHGD 1500
Query: 1501 EIPKFFTSQNMGNEGTVRIPERLYFAKCEVGVLVCAVVAINKQREKLERI-SELQSCVKV 1560
+IPKFF++Q+ GN +++P+ L + VG+ VCA+V ++K+R KL I E + KV
Sbjct: 1501 KIPKFFSNQSGGNMTEIKLPQYLEKFRESVGLAVCALVVVDKKRRKLNEIMPERERYTKV 1560
Query: 1561 LDLKCEFGVDSHEVEPEHHLFLPAEALSSQYEPSFIWLSCIPLAEFNVPWMCCDYLKSKV 1581
+DL C+F VDS+++ PEH F + L S+Y F+WLS IPL FN+ W C + +
Sbjct: 1561 VDLICKFKVDSYQIMPEHCHFTSQQKLLSEYASQFLWLSYIPLHGFNINWHYCTQFEVAL 1615
BLAST of Sgr016774 vs. ExPASy TrEMBL
Match:
A0A1S4E362 (TMV resistance protein N-like OS=Cucumis melo OX=3656 GN=LOC103498648 PE=4 SV=1)
HSP 1 Score: 1419.4 bits (3673), Expect = 0.0e+00
Identity = 804/1649 (48.76%), Postives = 1089/1649 (66.04%), Query Frame = 0
Query: 1 MEVPSSTPPNRPRMTYDVFISFRGEDTRNNFVGFLHEALRQLGIMTFLDDKKLLIGDNLS 60
M+ SS+ +RP+M YDVFISFRG D R+ F G+L++AL +LGI FLD+K+ LIGD+L
Sbjct: 1 MQSSSSSSLDRPKMNYDVFISFRGRDVRHTFAGYLYDALNRLGIKAFLDNKRFLIGDDL- 60
Query: 61 ENLVRAIEESRSSIVVLSKDYASSKWCLKELAKIIDCMAETTHRVLPVFYHVDPSNVRHQ 120
+L + I+ESRS+IVVLS+DYAS+KWCL+EL KI+D M + RVLPVFYH+DPS V+ Q
Sbjct: 61 HDLFKIIDESRSAIVVLSEDYASAKWCLRELTKIMDSMGTSMERVLPVFYHIDPSIVKDQ 120
Query: 121 SGSFKKSFDEHETIAHKFI-GEQKEKYLEEVQNWRDSMTKVGLLSGEVVTEHSPEVDIVK 180
SG+FK SFDEHE A K I ++KEK L+E+QNW++++ K+G +G V+T++S EVDIV
Sbjct: 121 SGTFKTSFDEHEANALKEIDNQEKEKRLKELQNWKNALKKIGNHTGVVITKNSSEVDIVN 180
Query: 181 KITNQIFERWRPKLVTSDKNLVGMTYRLLKMNVQLGLGLDDVRFLGIVGMGGIGKTTIAK 240
KI +QIF+ WRPKL +KNLVGMT RLL MN+ LGLGLDDVRF+ IVGMGGIGKTTIA+
Sbjct: 181 KIASQIFDAWRPKLEALNKNLVGMTSRLLHMNMHLGLGLDDVRFVAIVGMGGIGKTTIAQ 240
Query: 241 VVYDCITSKFEGSCFLRVSRGSSKQIELVKLQEQLLSRLFLKENVQIWDEDYGAEMIENQ 300
VV+DCI SKF+ CFL + G SKQ LV LQ ++LS++F KE+ +IW E++G EMI+N+
Sbjct: 241 VVFDCILSKFDDCCFLTLPGGDSKQ-SLVSLQREMLSQIFHKEDFKIWHENHGVEMIKNR 300
Query: 301 LRSRKVLLVLDGVEEKRQVEMLAGSPDWFGPGSRVIITTRNKEVL---QPYEILEYKVDL 360
L RKVL+VLDG EE+RQ+EMLAGS +WFGPGSR+IITTRNK +L E+ EY V+
Sbjct: 301 LSGRKVLIVLDGAEERRQLEMLAGSTEWFGPGSRIIITTRNKGLLCHPNYDEMKEYNVEE 360
Query: 361 LDSDSALQLFCNRAFGTDYVD-SNFMNLSKEIVEKLGRLPLALKVIGSYLNNKNGVIWNE 420
LD DSALQLF AFG+++ + +FM+LS EIVEK RLPLAL+VIGS L K IW E
Sbjct: 361 LDHDSALQLFLKHAFGSNHQNKDSFMDLSNEIVEKAKRLPLALRVIGSSLYGKEITIWRE 420
Query: 421 TLNRLIKVDEKYLFDVLKISYDGLGAESQKVFLDLACFFNGRRIDKVIKILESFGYNPHS 480
TL RLIKVDE+ FD+LKISYDGLG ESQ+VFLD+ CFFNG+ D+V +ILESFGY+P+S
Sbjct: 421 TLKRLIKVDERNFFDILKISYDGLGVESQQVFLDITCFFNGKNEDRVNEILESFGYSPNS 480
Query: 481 ELHLFMERCLIEVSHSKILMHDLILALGQEIVRKESLTQLGKQSRIWLQEDVIRAFTMKH 540
EL L M+RCLIEVSH KIL+HDLIL +G+EIVRKESLTQ KQSRIWL ED+ F KH
Sbjct: 481 ELQLLMQRCLIEVSHKKILVHDLILEMGREIVRKESLTQPEKQSRIWLHEDLYCRFAEKH 540
Query: 541 GLKYTEGIVLNLEKKQKE-LTLEAKLFADMTCLRILEISNVQLSGNIKSLSNLLGLLNWP 600
L + +GIVL+LEK+ +E + L+A+ F++MT LRILEI+NV+L +I+ LS LL ++NW
Sbjct: 541 DLMHIQGIVLSLEKEMEESIELDAESFSEMTKLRILEINNVELDEDIEYLSPLLRIINWL 600
Query: 601 GYPSKCLPSNFQSGYLLELHLCRSSIVRIWEGKKEFEKLKVINVSDSEYLLETPDFSEVP 660
GYPSK LP FQS YL EL L S ++R+W+GK+ F KLK+I+VS+SE+L TPDFS VP
Sbjct: 601 GYPSKSLPPTFQSRYLFELLLPHSQLLRVWDGKRRFPKLKLIDVSNSEHLRVTPDFSGVP 660
Query: 661 NLERLVLRNCRSLYHIHPSINSLSRLIFLDLNGCSSLKSFPSNLRCKNLERLVLSKSGVT 720
NLERLVL NC L IHPSINSL++LI LDL GC LK FP+N+RCKNL+ L LS +G+
Sbjct: 661 NLERLVLCNCVRLCEIHPSINSLNKLILLDLEGCGDLKHFPANIRCKNLQTLKLSGTGLE 720
Query: 721 SSLEIKGNMEHLCELRLDGTPIKQLHSSIERLVGLIVLDLRNCIMLSSLPNEIGNLKSLE 780
EI G+MEHL L LDG+ I LH SI L GL+ LDL C+ LSSLP EIGNLKSL+
Sbjct: 721 IFPEI-GHMEHLTHLHLDGSKITHLHPSIGYLTGLVFLDLSTCLGLSSLPFEIGNLKSLK 780
Query: 781 SLLLGGCVNLDQIPPSLGNAQCLAYLEISETSICRLPPT-IHCLKNLKGLNCDGLSRSIW 840
+LLL C LD+IPPSL NA+ L L ISETSI +P + IHCLKNL+ L+C+ LSR IW
Sbjct: 781 TLLLKYCKRLDKIPPSLANAESLETLSISETSITHVPSSIIHCLKNLETLDCEELSRGIW 840
Query: 841 PSLLRPCDILGSDLGISGSKFGLESLEYLTLKGCNLVNEDIPEDLHCFSSLKTLDLSGND 900
SLL +I + GL L+ L L GC L++EDIPEDLHCFSSL+TLDLS N+
Sbjct: 841 KSLLPQLNINQT------ITTGLGCLKALNLMGCKLMDEDIPEDLHCFSSLETLDLSYNN 900
Query: 901 FVRLPESVNHLKNLRELRLHDCFKLQHLPQLPTNLVPIVSYTESGTPVRSFP-------- 960
F LP+S++HLK L+ L L+ C +L+ LP+LP +L + G RS
Sbjct: 901 FTTLPDSLSHLKKLKTLILNYCTELKDLPKLPESLQYV-----GGVDCRSMSEQYYNKIL 960
Query: 961 -IPYQHGKHFILEF-IPKTNLKLD------------PKAFEESILKGVLFSSIDDKAEVL 1020
IP G L F IP + ++ ++FE+SI++ D ++
Sbjct: 961 LIPSSSGHQLYLTFIIPSKDADVECVMNEFQHSIFTRRSFEQSIIEKKPSPIFHDTVDMF 1020
Query: 1021 ECFDEIREGNLIEIEFYQ----------IKSDAN--NTYGVVLSACIQCSPNTTPEEVVT 1080
+ F +I EGN I++ Q + D N N GV LS I+ N +
Sbjct: 1021 QWFGQINEGNWTNIQYEQEFSISKPLNIMYEDVNLSNVCGVFLSTNIEFPQNLNHLAI-- 1080
Query: 1081 FYSKFFIELEMEENPRIGILPV-TSKLTSRTRFWMLFIPFH-------MLPKCRLRFKIV 1140
+F + E++ G + S+ + FW+ +IP M+ +C K+
Sbjct: 1081 --GRFLVSFEIDGKCSGGTMNYKMSQFKAARFFWVAYIPIWMNKDHSLMVQRC-CSVKVT 1140
Query: 1141 VKG-----SFPSVNVQKCG-ASILSHQNAPKFLANMFNMMYRTCNQNKFCTTIRECN-HG 1200
+ V ++ CG +S+LS N ++LA +F R C++ F T IR+ N H
Sbjct: 1141 ISYCCDHIDASKVKIKACGVSSMLSWPNVAEYLAKLFT--ERFCSKRNFYTMIRQHNDHQ 1200
Query: 1201 SECSCDEPET-VDQYCTTQYVENESDSFSLLKRNLKSILQRTYEEENHRSLVYFFPQGQV 1260
+EC CDE E D + ++ + N DS LL++NL++IL +E + + YFFP ++
Sbjct: 1201 TECRCDELEVGKDDFSSSTFESN--DSTFLLRKNLRAILGVMFEGKKRYYMKYFFPHTKI 1260
Query: 1261 SSWFRIQQPKDTVAVKLPLNLFREKKWMGLAVFAIFSIPGNAEFVHNHIFTCQISSDEDG 1320
WF+ Q KD VAVK+P+N+ +++KWMGLA+F +FSI +E + F +I + E+
Sbjct: 1261 FGWFKNQNKKDKVAVKIPVNIEKDRKWMGLAMFVVFSI---SEKASCYCFEYEIQTKENI 1320
Query: 1321 RHLMKGGSYSSHSFLIPLSLSQFSPQILFVTFKPRH-VFPYVQ---DGSNHFYVSFISKA 1380
S SHS L + S QILFV F+PR+ +PY + SNH Y++F +
Sbjct: 1321 I------STQSHSISTDQVL-EHSNQILFVAFEPRYNWYPYDELKSSSSNHVYINFNTNG 1380
Query: 1381 TRLRVESCGARLVYQHNVEGLIHTIMDCVLKSGQELFQEYSGKILSDALTTYERLIWIRV 1440
R+RVE CGARLVYQ NVEGL+HTI++C+ +SG EL++ Y+ I+ L T+ W +
Sbjct: 1381 ARMRVEFCGARLVYQQNVEGLVHTILNCIAESGDELYEHYNQYIVESHL-TFINTHWYTL 1440
Query: 1441 P-------ADEPTTSTSSDLSGHHSTNKLLHENFQRPLFKESTQDRYKSKFDAIIHGKEI 1500
++P+T+ S+ + S LL+ +F P F +S Q+R++SKFD ++HG +I
Sbjct: 1441 SFRRNNSVKNQPSTAASTCTASSLSVEHLLYGSFPHPFFHKSLQERFRSKFDLLLHGDKI 1500
Query: 1501 PKFFTSQNMGNEGTVRIPERLYFAKCEVGVLVCAVVAINKQREKLERI-SELQSCVKVLD 1560
PKFF++Q+ GN +++P+ L + VG+ VCA+V ++K+R KL I E + KV+D
Sbjct: 1501 PKFFSNQSGGNMTEIKLPQYLEEFRESVGLAVCALVVVDKKRRKLNEIMPERERYTKVVD 1560
Query: 1561 LKCEFGVDSHEVEPEHHLFLPAEALSSQYEPSFIWLSCIPLAEFNVPWMCCDYLKSKVSS 1581
L C+F VDS+++ PEH F + L S+Y F+WLS IPL FN+ W C + + +
Sbjct: 1561 LICKFKVDSYQIMPEHCHFTSQQKLLSEYASQFLWLSYIPLHGFNINWHYCTQFEVALET 1615
BLAST of Sgr016774 vs. TAIR 10
Match:
AT5G36930.1 (Disease resistance protein (TIR-NBS-LRR class) family )
HSP 1 Score: 511.1 bits (1315), Expect = 3.1e-144
Identity = 339/936 (36.22%), Postives = 489/936 (52.24%), Query Frame = 0
Query: 13 RMTYDVFISFRGEDTRNNFVGFLHEALRQLGIMTFLDDKKLLIGDNLSENLVRAIEESRS 72
R TYDVF+SFRG D R NF+ L+++LR+ GI TF+DD +L G+ +S L+ AIE S+
Sbjct: 11 RWTYDVFVSFRGADVRKNFLSHLYDSLRRCGISTFMDDVELQRGEYISPELLNAIETSKI 70
Query: 73 SIVVLSKDYASSKWCLKELAKIIDCMAET-THRVLPVFYHVDPSNVRHQSGSFKKSFDEH 132
IVVL+KDYASS WCL EL I+ +H V P+F +VDPS++R Q GS+ KSF +H
Sbjct: 71 LIVVLTKDYASSAWCLDELVHIMKSHKNNPSHMVFPIFLYVDPSDIRWQQGSYAKSFSKH 130
Query: 133 ETIAHKFIGEQKEKYLEEVQNWRDSMTKVGLLSGEVVTEHSPEVDIVKKITNQIFERWRP 192
+ +H L ++++WR+++TKV +SG + + E + + IT +I +R
Sbjct: 131 KN-SHP---------LNKLKDWREALTKVANISGWDIKNRN-EAECIADITREILKRLPC 190
Query: 193 KLVTSDKNLVGMTYRLLKMNVQLGLGLDDVRFLGIVGMGGIGKTTIAKVVYDCITSKFEG 252
+ + VG+ RL ++ L +G D VR + I GMGGIGKTT+AKV ++ + FEG
Sbjct: 191 QYLHVPSYAVGLRSRLQHISSLLSIGSDGVRVIVIYGMGGIGKTTLAKVAFNEFSHLFEG 250
Query: 253 SCFLRVSRGSSKQIE-LVKLQEQLLSRLFLKENVQIWDEDYGAEMIENQLRSRKVLLVLD 312
S FL R SK+ E LQ QLLS + + +++ D+ ++ + RS++VLLV+D
Sbjct: 251 SSFLENFREYSKKPEGRTHLQHQLLSDILRRNDIEFKGLDHA---VKERFRSKRVLLVVD 310
Query: 313 GVEEKRQVEMLAGSPDWFGPGSRVIITTRNKEVLQPYEIL-EYKVDLLDSDSALQLFCNR 372
V++ Q+ A D FG GSR+IITTRN +L+ Y LD D +L+LF
Sbjct: 311 DVDDVHQLNSAAIDRDCFGHGSRIIITTRNMHLLKQLRAEGSYSPKELDGDESLELFSWH 370
Query: 373 AFGTDYVDSNFMNLSKEIVEKLGRLPLALKVIGSYLNNKNGVIWNETLNRLIKVDEKYLF 432
AF T F+ S+E+V LPLA++V+G++L ++ W TL L ++ +
Sbjct: 371 AFRTSEPPKEFLQHSEEVVTYCAGLPLAVEVLGAFLIERSIREWESTLKLLKRIPNDNIQ 430
Query: 433 DVLKISYDGLGAESQKVFLDLACFFNGRRIDKVIKILESFGYNPHSELHLFMERCLIEVS 492
L+IS++ L E + VFLD+ACFF G V IL+ P L L MERCLI +S
Sbjct: 431 AKLQISFNALTIEQKDVFLDIACFFIGVDSYYVACILDGCNLYPDIVLSLLMERCLITIS 490
Query: 493 HSKILMHDLILALGQEIVRKESLTQLGKQSRIWLQEDVIRAFTMKHGLKYTEGIVLNLEK 552
+ I+MHDL+ +G++IVR+ S + G++SR+W DV+ K G EG+ L +
Sbjct: 491 GNNIMMHDLLRDMGRQIVREISPKKCGERSRLWSHNDVVGVLKKKSGTNAIEGLSLKADV 550
Query: 553 KQKELTLEAKLFADMTCLRILEISNVQLSGNIKSLSNLLGLLNWPGYPSKCLPSNFQSGY 612
+ E + FA M LR+LE+ V L+G+ + L L W G+ +C P N
Sbjct: 551 MDFQY-FEVEAFAKMQELRLLELRYVDLNGSYEHFPKDLRWLCWHGFSLECFPINLSLES 610
Query: 613 LLELHLCRSSIVRIWEGK---KEFEKLKVINVSDSEYLLETPDFSEVPNLERLVLRNCRS 672
L L L S++ R W+ + + +K +++S S YL ETPDFS PN+E+L+L NC+S
Sbjct: 611 LAALDLQYSNLKRFWKAQSPPQPANMVKYLDLSHSVYLRETPDFSYFPNVEKLILINCKS 670
Query: 673 LYHIHPSINSLSRLIFLDLNGCSSLKSFPSNLRCKNLERLVLSKSGVTSSLEIKGNMEHL 732
L +H SI L +
Sbjct: 671 LVLVHKSIGILDK----------------------------------------------- 730
Query: 733 CELRLDGTPIKQLHSSIERLVGLIVLDLRNCIMLSSLPNEIGNLKSLESLLLGGCVNLDQ 792
L++L+L +CI L LP EI LKSLESL L C L++
Sbjct: 731 ---------------------KLVLLNLSSCIELDVLPEEIYKLKSLESLFLSNCSKLER 790
Query: 793 IPPSLGNAQCLAYLEISETSICRLPPTIHCLKNLKGLN---CDGL----------SRSIW 852
+ +LG + L L T++ +P TI+ LK LK L+ C GL +S
Sbjct: 791 LDDALGELESLTTLLADFTALREIPSTINQLKKLKRLSLNGCKGLLSDDIDNLYSEKSHS 850
Query: 853 PSLLRPCDILGSDLGISGSKFGLESLEYLTLKGCNLVNEDIPEDLHCFSSLKTLDLSGND 912
SLLRP S GL + L+L CNL +E IPED+ S L+ LDL GN
Sbjct: 851 VSLLRPV-----------SLSGLTYMRILSLGYCNLSDELIPEDIGSLSFLRDLDLRGNS 852
Query: 913 FVRLPESVNHLKNLRELRLHDCFKLQHLPQLPTNLV 930
F LP L NL EL L DC KLQ + LP +L+
Sbjct: 911 FCNLPTDFATLPNLGELLLSDCSKLQSILSLPRSLL 852
BLAST of Sgr016774 vs. TAIR 10
Match:
AT5G36930.2 (Disease resistance protein (TIR-NBS-LRR class) family )
HSP 1 Score: 511.1 bits (1315), Expect = 3.1e-144
Identity = 339/936 (36.22%), Postives = 489/936 (52.24%), Query Frame = 0
Query: 13 RMTYDVFISFRGEDTRNNFVGFLHEALRQLGIMTFLDDKKLLIGDNLSENLVRAIEESRS 72
R TYDVF+SFRG D R NF+ L+++LR+ GI TF+DD +L G+ +S L+ AIE S+
Sbjct: 14 RWTYDVFVSFRGADVRKNFLSHLYDSLRRCGISTFMDDVELQRGEYISPELLNAIETSKI 73
Query: 73 SIVVLSKDYASSKWCLKELAKIIDCMAET-THRVLPVFYHVDPSNVRHQSGSFKKSFDEH 132
IVVL+KDYASS WCL EL I+ +H V P+F +VDPS++R Q GS+ KSF +H
Sbjct: 74 LIVVLTKDYASSAWCLDELVHIMKSHKNNPSHMVFPIFLYVDPSDIRWQQGSYAKSFSKH 133
Query: 133 ETIAHKFIGEQKEKYLEEVQNWRDSMTKVGLLSGEVVTEHSPEVDIVKKITNQIFERWRP 192
+ +H L ++++WR+++TKV +SG + + E + + IT +I +R
Sbjct: 134 KN-SHP---------LNKLKDWREALTKVANISGWDIKNRN-EAECIADITREILKRLPC 193
Query: 193 KLVTSDKNLVGMTYRLLKMNVQLGLGLDDVRFLGIVGMGGIGKTTIAKVVYDCITSKFEG 252
+ + VG+ RL ++ L +G D VR + I GMGGIGKTT+AKV ++ + FEG
Sbjct: 194 QYLHVPSYAVGLRSRLQHISSLLSIGSDGVRVIVIYGMGGIGKTTLAKVAFNEFSHLFEG 253
Query: 253 SCFLRVSRGSSKQIE-LVKLQEQLLSRLFLKENVQIWDEDYGAEMIENQLRSRKVLLVLD 312
S FL R SK+ E LQ QLLS + + +++ D+ ++ + RS++VLLV+D
Sbjct: 254 SSFLENFREYSKKPEGRTHLQHQLLSDILRRNDIEFKGLDHA---VKERFRSKRVLLVVD 313
Query: 313 GVEEKRQVEMLAGSPDWFGPGSRVIITTRNKEVLQPYEIL-EYKVDLLDSDSALQLFCNR 372
V++ Q+ A D FG GSR+IITTRN +L+ Y LD D +L+LF
Sbjct: 314 DVDDVHQLNSAAIDRDCFGHGSRIIITTRNMHLLKQLRAEGSYSPKELDGDESLELFSWH 373
Query: 373 AFGTDYVDSNFMNLSKEIVEKLGRLPLALKVIGSYLNNKNGVIWNETLNRLIKVDEKYLF 432
AF T F+ S+E+V LPLA++V+G++L ++ W TL L ++ +
Sbjct: 374 AFRTSEPPKEFLQHSEEVVTYCAGLPLAVEVLGAFLIERSIREWESTLKLLKRIPNDNIQ 433
Query: 433 DVLKISYDGLGAESQKVFLDLACFFNGRRIDKVIKILESFGYNPHSELHLFMERCLIEVS 492
L+IS++ L E + VFLD+ACFF G V IL+ P L L MERCLI +S
Sbjct: 434 AKLQISFNALTIEQKDVFLDIACFFIGVDSYYVACILDGCNLYPDIVLSLLMERCLITIS 493
Query: 493 HSKILMHDLILALGQEIVRKESLTQLGKQSRIWLQEDVIRAFTMKHGLKYTEGIVLNLEK 552
+ I+MHDL+ +G++IVR+ S + G++SR+W DV+ K G EG+ L +
Sbjct: 494 GNNIMMHDLLRDMGRQIVREISPKKCGERSRLWSHNDVVGVLKKKSGTNAIEGLSLKADV 553
Query: 553 KQKELTLEAKLFADMTCLRILEISNVQLSGNIKSLSNLLGLLNWPGYPSKCLPSNFQSGY 612
+ E + FA M LR+LE+ V L+G+ + L L W G+ +C P N
Sbjct: 554 MDFQY-FEVEAFAKMQELRLLELRYVDLNGSYEHFPKDLRWLCWHGFSLECFPINLSLES 613
Query: 613 LLELHLCRSSIVRIWEGK---KEFEKLKVINVSDSEYLLETPDFSEVPNLERLVLRNCRS 672
L L L S++ R W+ + + +K +++S S YL ETPDFS PN+E+L+L NC+S
Sbjct: 614 LAALDLQYSNLKRFWKAQSPPQPANMVKYLDLSHSVYLRETPDFSYFPNVEKLILINCKS 673
Query: 673 LYHIHPSINSLSRLIFLDLNGCSSLKSFPSNLRCKNLERLVLSKSGVTSSLEIKGNMEHL 732
L +H SI L +
Sbjct: 674 LVLVHKSIGILDK----------------------------------------------- 733
Query: 733 CELRLDGTPIKQLHSSIERLVGLIVLDLRNCIMLSSLPNEIGNLKSLESLLLGGCVNLDQ 792
L++L+L +CI L LP EI LKSLESL L C L++
Sbjct: 734 ---------------------KLVLLNLSSCIELDVLPEEIYKLKSLESLFLSNCSKLER 793
Query: 793 IPPSLGNAQCLAYLEISETSICRLPPTIHCLKNLKGLN---CDGL----------SRSIW 852
+ +LG + L L T++ +P TI+ LK LK L+ C GL +S
Sbjct: 794 LDDALGELESLTTLLADFTALREIPSTINQLKKLKRLSLNGCKGLLSDDIDNLYSEKSHS 853
Query: 853 PSLLRPCDILGSDLGISGSKFGLESLEYLTLKGCNLVNEDIPEDLHCFSSLKTLDLSGND 912
SLLRP S GL + L+L CNL +E IPED+ S L+ LDL GN
Sbjct: 854 VSLLRPV-----------SLSGLTYMRILSLGYCNLSDELIPEDIGSLSFLRDLDLRGNS 855
Query: 913 FVRLPESVNHLKNLRELRLHDCFKLQHLPQLPTNLV 930
F LP L NL EL L DC KLQ + LP +L+
Sbjct: 914 FCNLPTDFATLPNLGELLLSDCSKLQSILSLPRSLL 855
BLAST of Sgr016774 vs. TAIR 10
Match:
AT5G17680.1 (disease resistance protein (TIR-NBS-LRR class), putative )
HSP 1 Score: 503.1 bits (1294), Expect = 8.4e-142
Identity = 318/927 (34.30%), Postives = 492/927 (53.07%), Query Frame = 0
Query: 17 DVFISFRGEDTRNNFVGFLHEALRQLGIMTFLDDKKLLIGDNLSENLVRAIEESRSSIVV 76
DVF+SFRGED R FV L ++GI F DD L G ++S L+ AI+ SR +IVV
Sbjct: 19 DVFVSFRGEDVRKTFVSHLFCEFDRMGIKAFRDDLDLQRGKSISPELIDAIKGSRFAIVV 78
Query: 77 LSKDYASSKWCLKELAKIIDCMAETTHRVLPVFYHVDPSNVRHQSGSFKKSFDEHETIAH 136
+S++YA+S WCL EL KI++C +T ++P+FY VDPS+VR Q GSF + + H
Sbjct: 79 VSRNYAASSWCLDELLKIMECNKDT---IVPIFYEVDPSDVRRQRGSFGEDVESH----- 138
Query: 137 KFIGEQKEKYLEEVQNWRDSMTKVGLLSGEVVTEHSPEVDIVKKITNQIFERWRPKLVTS 196
KEK V W++++ K+ +SGE + + + ++KKI I ++
Sbjct: 139 ----SDKEK----VGKWKEALKKLAAISGE-DSRNWDDSKLIKKIVKDISDKLVSTSWDD 198
Query: 197 DKNLVGMTYRLLKMNVQLGLGLDDVRFLGIVGMGGIGKTTIAKVVYDCITSKFEGSCFLR 256
K L+GM+ + + + + DVR LGI GMGG+GKTTIAK +Y+ ++ +F+ CF+
Sbjct: 199 SKGLIGMSSHMDFLQSMISIVDKDVRMLGIWGMGGVGKTTIAKYLYNQLSGQFQVHCFME 258
Query: 257 VSRGSSKQIELVKLQEQLLSRLFLKENVQIWDEDYGAEMIENQLRSRKVLLVLDGVEEKR 316
+ + + +LQ + L R+F + + + W +I+ + R + V +VLD V+
Sbjct: 259 NVKEVCNRYGVRRLQVEFLCRMFQERDKEAWSSVSCCNIIKERFRHKMVFIVLDDVDRSE 318
Query: 317 QVEMLAGSPDWFGPGSRVIITTRNKEVLQPYEI-LEYKVDLLDSDSALQLFCNRAFGTDY 376
Q+ L WFGPGSR+I+TTR++ +L + I L YKV L ALQLFCN AF +
Sbjct: 319 QLNELVKETGWFGPGSRIIVTTRDRHLLLSHGINLVYKVKCLPKKEALQLFCNYAFREEI 378
Query: 377 V-DSNFMNLSKEIVEKLGRLPLALKVIGSYLNNKNGVIWNETLNRLIKVDEKYLFDVLKI 436
+ F LS + V LPLAL+V+GS+L ++ + W TL RL + +VL++
Sbjct: 379 ILPHGFEELSVQAVNYASGLPLALRVLGSFLYRRSQIEWESTLARLKTYPHSDIMEVLRV 438
Query: 437 SYDGLGAESQKVFLDLACFFNGRRIDKVIKILESFGYNPHSELHLFMERCLIEVSHSKIL 496
SYDGL + + +FL ++CF+N +++D V K+L+ GY + + E+ LI S+ +
Sbjct: 439 SYDGLDEQEKAIFLYISCFYNMKQVDYVRKLLDLCGYAAEIGITILTEKSLIVESNGCVK 498
Query: 497 MHDLILALGQEIVRKESLTQLGKQSRIWLQEDVIRAFTMKHGLKYTEGIVLNLEKKQKEL 556
+HDL+ +G+E+VR++++ ++ +W ED+ + G + EGI LNL + E+
Sbjct: 499 IHDLLEQMGRELVRQQAVNNPAQRLLLWDPEDICHLLSENSGTQLVEGISLNL-SEISEV 558
Query: 557 TLEAKLFADMTCLRILEI--------SNVQLSGNIKSLSNLLGLLNWPGYPSKCLPSNFQ 616
+ F ++ L++L + V L + L L L W GYP K +PS F
Sbjct: 559 FASDRAFEGLSNLKLLNFYDLSFDGETRVHLPNGLSYLPRKLRYLRWDGYPLKTMPSRFF 618
Query: 617 SGYLLELHLCRSSIVRIWEGKKEFEKLKVINVSDSEYLLETPDFSEVPNLERLVLRNCRS 676
+L+EL + S++ ++W+G + LK +++S +YL+E PD S+ NLE L L C+S
Sbjct: 619 PEFLVELCMSNSNLEKLWDGIQPLRNLKKMDLSRCKYLVEVPDLSKATNLEELNLSYCQS 678
Query: 677 LYHIHPSINSLSRLIFLDLNGCSSLKSFPSNLRCKNLERLVLSK-SGVTSSLEIKGNMEH 736
L + PSI +L L L C LK P + K+LE + +S S + EI N
Sbjct: 679 LVEVTPSIKNLKGLSCFYLTNCIQLKDIPIGIILKSLETVGMSGCSSLKHFPEISWNTR- 738
Query: 737 LCELRLDGTPIKQLHSSIERLVGLIVLDLRNCIMLSSLPNEIGNLKSLESLLLGGCVNLD 796
L L T I++L SSI RL L+ LD+ +C L +LP+ +G+L SL+SL L GC L+
Sbjct: 739 --RLYLSSTKIEELPSSISRLSCLVKLDMSDCQRLRTLPSYLGHLVSLKSLNLDGCRRLE 798
Query: 797 QIPPSLGNAQCLAYLE---------------------ISETSICRLPPTIHCLKNLKGLN 856
+P +L N L LE ISETSI +P I L L+ L+
Sbjct: 799 NLPDTLQNLTSLETLEVSGCLNVNEFPRVSTSIEVLRISETSIEEIPARICNLSQLRSLD 858
Query: 857 CDGLSRSIWPSLLRPCDILGSDLGISGSKFGLESLEYLTLKGCNLVNEDIPEDLHCFSSL 912
R + L +S S+ L SLE L L GC+++ E S L
Sbjct: 859 ISENKRL-------------ASLPVSISE--LRSLEKLKLSGCSVLESFPLEICQTMSCL 909
BLAST of Sgr016774 vs. TAIR 10
Match:
AT4G12010.1 (Disease resistance protein (TIR-NBS-LRR class) family )
HSP 1 Score: 492.3 bits (1266), Expect = 1.5e-138
Identity = 325/940 (34.57%), Postives = 500/940 (53.19%), Query Frame = 0
Query: 16 YDVFISFRGEDTRNNFVGFLHEALRQLGIMTFLDDKKLLIGDNLSENLVRAIEESRSSIV 75
+DVF+SFRG DTRNNF G L +ALR GI +F+DD +L GDNL+ L IE+S+ +I+
Sbjct: 11 FDVFLSFRGFDTRNNFTGHLQKALRLRGIDSFIDD-RLRRGDNLTA-LFDRIEKSKIAII 70
Query: 76 VLSKDYASSKWCLKELAKIIDCMAETTHRVLPVFYHVDPSNVRHQSGSFKKSFDEHETIA 135
V S +YA+S WCL+EL KI++C V+P+FY VD S+V Q SF F E
Sbjct: 71 VFSTNYANSAWCLRELVKILECRNSNQQLVVPIFYKVDKSDVEKQRNSFAVPFKLPEL-- 130
Query: 136 HKFIGEQKEKYLEEVQNWRDSMTKVGLLSGEVVTEHS-PEVDIVKKITNQIFERWRPKLV 195
F G EE+ +W+ ++ + G VV E S E +V +I F++
Sbjct: 131 -TFPGVTP----EEISSWKAALASASNILGYVVKEISTSEAKLVDEIAVDTFKKLNDLAP 190
Query: 196 TSDKNLVGMTYRLLKMNVQLGL-GLDDVRFLGIVGMGGIGKTTIAKVVYDCITSKFEGSC 255
+ ++ LVG+ RL + L LD V +GIVGM GIGKTT+A +Y + +F+GSC
Sbjct: 191 SGNEGLVGIESRLKNLEKLLSWEDLDTVHIIGIVGMVGIGKTTLADCLYGRMRGQFDGSC 250
Query: 256 FLRVSRGSSKQIELVKLQEQLLSRLFLKENVQIWDEDYGAEMIENQLRSRKVLLVLDGVE 315
FL R +S + L L ++L S + +++I E E +L+S+++L+VLD V
Sbjct: 251 FLTNIRENSGRSGLESLLQKLFSTVLNDRDLEIGAPGNAHERFERRLKSKRLLIVLDDVN 310
Query: 316 EKRQVEMLAGSPDWFGPGSRVIITTRNKEVLQPYEILEYKVDLLDSDSALQLFCNRAFGT 375
+++Q+ L G W+ GSR+IITTR+ ++++ + +Y + L+ AL+LF AF
Sbjct: 311 DEKQIRYLMGHCKWYQGGSRIIITTRDSKLIETIKGRKYVLPKLNDREALKLFSLNAFSN 370
Query: 376 DYVDSNFMNLSKEIVEKLGRLPLALKVIGSYLNNKNGVIWNETLNRLIKVDEKYLFDVLK 435
+ F L+ +++ PLALKV+GS L ++ + W L+RL +++VL+
Sbjct: 371 SFPLKEFEGLTNMVLDYAKGHPLALKVLGSDLCERDDLYWEAKLDRLKSRSHGDIYEVLE 430
Query: 436 ISYDGLGAESQKVFLDLACFFNGRRIDKVIKILESFGYNPHSELHLFMERCLIEVSHSKI 495
SY+ L E + VFLD+ACFF +D V +L S G + + +++CLI +S ++I
Sbjct: 431 TSYEELTTEQKNVFLDIACFFRSENVDYVTSLLNSHGVDVSGVVKDLVDKCLITLSDNRI 490
Query: 496 LMHDLILALGQEIVRKES---------LTQLGKQS----RIWLQEDVIRAFTMKHGLKYT 555
MHD++ + +EI K L++ G Q R+W ED+ T G
Sbjct: 491 EMHDMLQTMAKEISLKVETIGIRDCRWLSRHGNQCQWHIRLWDSEDICDLLTEGLGTDKI 550
Query: 556 EGIVLNLEKKQKELTLEAKLFADMTCLRILEISN------------VQLSGNIKSLSNLL 615
GI L+ K + + L AK F M L+ L+I + + L + L N L
Sbjct: 551 RGIFLD-TSKLRAMRLSAKAFQGMYNLKYLKIYDSHCSRGCEAEFKLHLRRGLSFLPNEL 610
Query: 616 GLLNWPGYPSKCLPSNFQSGYLLELHLCRSSIVRIWEGKKEFEKLKVINVSDSEYLLETP 675
L+W GYP + +P +F L++L L S + IW+ +K+ LK +++S S L +
Sbjct: 611 TYLHWHGYPLQSIPLDFDPKNLVDLKLPHSQLEEIWDDEKDVGMLKWVDLSHSINLRQCL 670
Query: 676 DFSEVPNLERLVLRNCRSLYHIHPSINSLSRLIFLDLNGCSSLKSFPSNLRCKNLERLVL 735
+ NLERL L C SL + +IN L +LI+L+L C+SL+S P ++ ++L+ L+L
Sbjct: 671 GLANAHNLERLNLEGCTSLKKLPSTINCLEKLIYLNLRDCTSLRSLPKGIKTQSLQTLIL 730
Query: 736 SKSGVTSSLEIKGNMEHLCELRLDGTPIKQLHSSIERLVGLIVLDLRNCIMLSSLPNEIG 795
SG +S + E++ L LDGT IK L SI+ L +L+L+NC L L +++
Sbjct: 731 --SGCSSLKKFPLISENVEVLLLDGTVIKSLPESIQTFRRLALLNLKNCKKLKHLSSDLY 790
Query: 796 NLKSLESLLLGGCVNLDQIPPSLGNAQCLAYLEISETSICRLPPTIHCLKNLKGLNCDGL 855
LK L+ L+L GC L+ P + + L L + +TSI +P +H L N+K + G
Sbjct: 791 KLKCLQELILSGCSQLEVFPEIKEDMESLEILLMDDTSITEMPKMMH-LSNIKTFSLCGT 850
Query: 856 SRSIWPSLLRPCDILGSDLGISGSKFGLESLEYLTLKGCNLVNEDIPEDLHCFSSLKTLD 915
S + S+ G L L L C+L +P+++ SSL++L
Sbjct: 851 SSHVSVSMF-----------FMPPTLGCSRLTDLYLSRCSLYK--LPDNIGGLSSLQSLC 910
Query: 916 LSGNDFVRLPESVNHLKNLRELRLHDCFKLQHLPQLPTNL 929
LSGN+ LPES N L NL+ L C L+ LP LP NL
Sbjct: 911 LSGNNIENLPESFNQLNNLKWFDLKFCKMLKSLPVLPQNL 924
BLAST of Sgr016774 vs. TAIR 10
Match:
AT1G27170.1 (transmembrane receptors;ATP binding )
HSP 1 Score: 480.3 bits (1235), Expect = 5.8e-135
Identity = 345/1037 (33.27%), Postives = 526/1037 (50.72%), Query Frame = 0
Query: 13 RMTYDVFISFRGEDTRNNFVGFLHEALRQLGIMTFLDDKKLLIGDNLSENLVRAIEESRS 72
R+ YDVF+SFRG DTR+NF L++AL+ + F D++ + GD +S +L +E+S +
Sbjct: 11 RLKYDVFLSFRGADTRDNFGDHLYKALKD-KVRVFRDNEGMERGDEISSSLKAGMEDSAA 70
Query: 73 SIVVLSKDYASSKWCLKELAKIIDCMAETTHRVLPVFYHVDPSNVRHQSGSFKKSFDEHE 132
S++V+S++Y+ S+WCL ELA + + R+LP+FYHVDPS+VR QS KK F+EH+
Sbjct: 71 SVIVISRNYSGSRWCLDELAMLCKMKSSLDRRILPIFYHVDPSHVRKQSDHIKKDFEEHQ 130
Query: 133 TIAHKFIGEQKEKYLEEVQNWRDSMTKVGLLSGEVVTEHSPEVDIVKKITNQIFERWRPK 192
E+KEK VQ WR+++T VG L+G V + S + D+++ + ++
Sbjct: 131 V----RFSEEKEK----VQEWREALTLVGNLAGYVCDKDSKDDDMIELVVKRVLAELSNT 190
Query: 193 LVTSDKNLVGMTYRLLKMNVQLGL-GLDDVRFLGIVGMGGIGKTTIAKVVYDCITSKFEG 252
+ +VG+ L + + V+ LG+ GMGGIGKTT+AK Y+ I FE
Sbjct: 191 PEKVGEFIVGLESPLKDLTGLIDTESSSGVQVLGLYGMGGIGKTTLAKAFYNKIVGNFEQ 250
Query: 253 SCFLR-VSRGSSKQIELVKLQEQLLSRLFLKENVQIWDEDYGAEMIENQLRSRKVLLVLD 312
F+ + SS + LV LQ+ L+ LF + +I D G E I+ + +K+++VLD
Sbjct: 251 RAFISDIRERSSAENGLVTLQKTLIKELF-RLVPEIEDVSIGLEKIKANVHEKKIIVVLD 310
Query: 313 GVEEKRQVEMLAGSPDWFGPGSRVIITTRNKEVLQPYEI-LEYKVDLLDSDSALQLFCNR 372
V+ QV L G W+G G+ ++ITTR+ E+L + +Y+V L AL+LF
Sbjct: 311 DVDHIDQVHALVGETRWYGQGTLIVITTRDSEILSKLSVNQQYEVKCLTEPQALKLFSYH 370
Query: 373 AFGTDYVDSNFMNLSKEIVEKLGRLPLALKVIGSYL-NNKNGVIWNETLNRLIKVDEKYL 432
+ + N + LSK+IV+ G LPLA++V GS L + K W L++L K L
Sbjct: 371 SLRKEEPTKNLLALSKKIVQISGLLPLAVEVFGSLLYDKKEEKDWQTQLDKLKKTQPGNL 430
Query: 433 FDVLKISYDGLGAESQKVFLDLACFFNGRRI--DKVIKILESFGYNPHSELHLFMERCLI 492
DVL++S+ L E +KVFLD+AC F I D+V+ +L+ G N + L + ++ L+
Sbjct: 431 QDVLELSFKSLDDEEKKVFLDIACLFLKMEIKKDEVVIVLKGCGLNAEAALSVLRQKSLV 490
Query: 493 EV-SHSKILMHDLILALGQEIVRKESLTQLGKQSRIWLQEDVIRAFTMKHGLKYTEGIVL 552
++ ++ + MHD I +G+++V KES G +SR+W + +++ G GIVL
Sbjct: 491 KILANDTLWMHDQIRDMGRQMVLKESREDPGLRSRLWDRGEIMTVLNNMKGTSSIRGIVL 550
Query: 553 NLEK------------------------------------------KQKELTLEAKLFAD 612
+ +K K E+T+ + FA
Sbjct: 551 DFKKKFARDPTADEIVSRNLRNNPGIYSVFNYLKNKLVRFPAEEKPKSSEITIPVESFAP 610
Query: 613 MTCLRILEISNVQLSGNIKSLSNLLGLLNWPGYPSKCLPSNFQSGYLLELHLCRSSI--V 672
MT LR+L+I+NV+L GN+K L + L + W G P + LP +F + L L L S I V
Sbjct: 611 MTKLRLLQINNVELEGNLKLLPSELKWIQWKGCPLENLPPDFLARQLSVLDLSESGIRQV 670
Query: 673 RIWEGKKEFEKLKVINVSDSEYLLETPDFSEVPNLERLVLRNCRSLYHIHPSINSLSRLI 732
+ K E LKV+ + L PD S LE+LV C L + S+ +L +LI
Sbjct: 671 QTLRNKMVDENLKVVILRGCHSLEAIPDLSNHEALEKLVFEQCTLLVKVPKSVGNLRKLI 730
Query: 733 FLDLNGCSSLKSFPSNLR-CKNLERLVLSKSGVTSSL-EIKGNMEHLCELRLDGTPIKQL 792
LD CS L F ++ K LE+L LS S L E G M L EL LDGT IK L
Sbjct: 731 HLDFRRCSKLSEFLVDVSGLKLLEKLFLSGCSDLSVLPENIGAMTSLKELLLDGTAIKNL 790
Query: 793 HSSIERLVGLIVLDLRNCIMLSSLPNEIGNLKSLESLLLGGCVNLDQIPPSLGNAQCLAY 852
SI RL L +L LR C + LP IG LKSLE L L L +P S+G+ + L
Sbjct: 791 PESINRLQNLEILSLRGC-KIQELPLCIGTLKSLEKLYLDDTA-LKNLPSSIGDLKNLQD 850
Query: 853 LEISE-TSICRLPPTIHCLKNLKGLNCDGLSRSIWPSLLRPCDILGSDLGISGSKFGLES 912
L + TS+ ++P +I+ LK+LK L +G + P L+P L S
Sbjct: 851 LHLVRCTSLSKIPDSINELKSLKKLFINGSAVEELP--LKPS--------------SLPS 910
Query: 913 LEYLTLKGCNLVNEDIPEDLHCFSSLKTLDLSGNDFVRLPESVNHLKNLRELRLHDCFKL 972
L + C + + +P + +SL L LS LPE + L +REL L +C L
Sbjct: 911 LYDFSAGDCKFLKQ-VPSSIGRLNSLLQLQLSSTPIEALPEEIGALHFIRELELRNCKFL 970
Query: 973 QHLPQLPTNLVPIVSYTESGTPVRSFPIPYQHGKHFILEFIPKTNLKL---------DPK 987
+ LP+ ++ + S G+ + +P + GK L + +N K+ D K
Sbjct: 971 KFLPKSIGDMDTLYSLNLEGSNIEE--LPEEFGKLEKLVELRMSNCKMLKRLPESFGDLK 1016
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| XP_022141775.1 | 0.0e+00 | 82.36 | TMV resistance protein N-like [Momordica charantia] | [more] |
| XP_022141862.1 | 0.0e+00 | 71.05 | TMV resistance protein N-like [Momordica charantia] | [more] |
| XP_022141776.1 | 0.0e+00 | 77.05 | LOW QUALITY PROTEIN: TMV resistance protein N-like [Momordica charantia] | [more] |
| XP_038890520.1 | 0.0e+00 | 50.03 | TMV resistance protein N-like isoform X1 [Benincasa hispida] >XP_038890521.1 TMV... | [more] |
| KAA0039330.1 | 0.0e+00 | 48.94 | TMV resistance protein N-like [Cucumis melo var. makuwa] >TYK00512.1 TMV resista... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| V9M2S5 | 2.6e-164 | 38.21 | Disease resistance protein RPV1 OS=Vitis rotundifolia OX=103349 GN=RPV1 PE=1 SV=... | [more] |
| Q40392 | 3.6e-153 | 36.36 | TMV resistance protein N OS=Nicotiana glutinosa OX=35889 GN=N PE=1 SV=1 | [more] |
| V9M398 | 6.7e-152 | 37.12 | Disease resistance protein RUN1 OS=Vitis rotundifolia OX=103349 GN=RUN1 PE=1 SV=... | [more] |
| A0A290U7C4 | 4.5e-140 | 34.97 | Disease resistance protein Roq1 OS=Nicotiana benthamiana OX=4100 GN=ROQ1 PE=1 SV... | [more] |
| Q9SZ66 | 2.1e-137 | 34.57 | Disease resistance-like protein DSC1 OS=Arabidopsis thaliana OX=3702 GN=DSC1 PE=... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1CJ31 | 0.0e+00 | 82.36 | TMV resistance protein N-like OS=Momordica charantia OX=3673 GN=LOC111012055 PE=... | [more] |
| A0A6J1CL15 | 0.0e+00 | 71.05 | TMV resistance protein N-like OS=Momordica charantia OX=3673 GN=LOC111012122 PE=... | [more] |
| A0A6J1CK95 | 0.0e+00 | 77.05 | LOW QUALITY PROTEIN: TMV resistance protein N-like OS=Momordica charantia OX=367... | [more] |
| A0A5A7T7V5 | 0.0e+00 | 48.94 | TMV resistance protein N-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... | [more] |
| A0A1S4E362 | 0.0e+00 | 48.76 | TMV resistance protein N-like OS=Cucumis melo OX=3656 GN=LOC103498648 PE=4 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| AT5G36930.1 | 3.1e-144 | 36.22 | Disease resistance protein (TIR-NBS-LRR class) family | [more] |
| AT5G36930.2 | 3.1e-144 | 36.22 | Disease resistance protein (TIR-NBS-LRR class) family | [more] |
| AT5G17680.1 | 8.4e-142 | 34.30 | disease resistance protein (TIR-NBS-LRR class), putative | [more] |
| AT4G12010.1 | 1.5e-138 | 34.57 | Disease resistance protein (TIR-NBS-LRR class) family | [more] |
| AT1G27170.1 | 5.8e-135 | 33.27 | transmembrane receptors;ATP binding | [more] |
