Sgr016701 (gene) Monk fruit (Qingpiguo) v1

Overview


Name	Sgr016701
Type	gene
Organism	Siraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description	Protein TIC 20
Location	tig00152985: 1294526 .. 1295219 (+)
RNA-Seq Expression	Sgr016701
Synteny	Sgr016701

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTAGACGACCCGCTTTCTTCGCCCTCAACCAACGCCTCTGCGCTGCTGCTATCTCAATTAATTCAAAAATGGCTTCCAGTTCCATGCCTCTCCTCAATCTCTCCTCCCACACCGCCATTAAAACCGCCCTATTCCCTCACTCCTCCGCCATCAAAGCTCGCCACCGTTCTTCCTCCCCCTCCTCTCCCCGAAACCACCAAAATCCCCCACCATTCGCATGTCCTACAACCCAACTCCGGCCACCGACCGACTAATCTCCGCCGTCGCCTACACTCTCCCTTCTTCAACTCCCTCCAGTACGGCCGCTTCCTCTTCGCCCAATATCCGACCCTCGGTCTCGCATTCGAGCCGCTAGTCCCCATCCTAGGGCTGTATCGGTCCATTCCGCACTCGAGCTTCGTCGCCTTCTTCGCGCTCTACTTGGGGGTGGTGCGGAACCCTAGCTTCAGCCAGTACGTGAGGTTCAATTCGATGCAGGCGGTGACTCTGGACGTGCTGCTGGTGGTGCCATTGTTGATCCAGAGGATTCTGAGTCCGGGTCGGGGCGGAATCGGGTTCCGGATCATGGTATGGGGGCACAATGGATTGTTCGTGTTCAGTGTTCTGTGCTTCGTTTACAGTCTGGTTTCTTGCCTTCTGGGTCGAACCCCTCACTTGCCCCTTGTTGCTGATGCTGCTGGGAGGCAGATTTAG

mRNA sequence

TTTAGACGACCCGCTTTCTTCGCCCTCAACCAACGCCTCTGCGCTGCTGCTATCTCAATTAATTCAAAAATGGCTTCCAGTTCCATGCCTCTCCTCAATCTCTCCTCCCACACCGCCATTAAAACCGCCCTATTCCCTCACTCCTCCGCCATCAAAGCTCGCCACCGTTCTTCCTCCCCCTCCTCTCCCCGAAACCACCAAAATCCCCCACCATTCGCATGTCCTACAACCCAACTCCGGCCACCGACCGACTAATCTCCGCCGTCGCCTACACTCTCCCTTCTTCAACTCCCTCCAGTACGGCCGCTTCCTCTTCGCCCAATATCCGACCCTCGGTCTCGCATTCGAGCCGCTAGTCCCCATCCTAGGGCTGTATCGGTCCATTCCGCACTCGAGCTTCGTCGCCTTCTTCGCGCTCTACTTGGGGGTGGTGCGGAACCCTAGCTTCAGCCAGTACGTGAGGTTCAATTCGATGCAGGCGGTGACTCTGGACGTGCTGCTGGTGGTGCCATTGTTGATCCAGAGGATTCTGAGTCCGGGTCGGGGCGGAATCGGGTTCCGGATCATGGTATGGGGGCACAATGGATTGTTCGTGTTCAGTGTTCTGTGCTTCGTTTACAGTCTGGTTTCTTGCCTTCTGGGTCGAACCCCTCACTTGCCCCTTGTTGCTGATGCTGCTGGGAGGCAGATTTAG

Coding sequence (CDS)

TTTAGACGACCCGCTTTCTTCGCCCTCAACCAACGCCTCTGCGCTGCTGCTATCTCAATTAATTCAAAAATGGCTTCCAGTTCCATGCCTCTCCTCAATCTCTCCTCCCACACCGCCATTAAAACCGCCCTATTCCCTCACTCCTCCGCCATCAAAGCTCGCCACCGTTCTTCCTCCCCCTCCTCTCCCCGAAACCACCAAAATCCCCCACCATTCGCATGTCCTACAACCCAACTCCGGCCACCGACCGACTAATCTCCGCCGTCGCCTACACTCTCCCTTCTTCAACTCCCTCCAGTACGGCCGCTTCCTCTTCGCCCAATATCCGACCCTCGGTCTCGCATTCGAGCCGCTAGTCCCCATCCTAGGGCTGTATCGGTCCATTCCGCACTCGAGCTTCGTCGCCTTCTTCGCGCTCTACTTGGGGGTGGTGCGGAACCCTAGCTTCAGCCAGTACGTGAGGTTCAATTCGATGCAGGCGGTGACTCTGGACGTGCTGCTGGTGGTGCCATTGTTGATCCAGAGGATTCTGAGTCCGGGTCGGGGCGGAATCGGGTTCCGGATCATGGTATGGGGGCACAATGGATTGTTCGTGTTCAGTGTTCTGTGCTTCGTTTACAGTCTGGTTTCTTGCCTTCTGGGTCGAACCCCTCACTTGCCCCTTGTTGCTGATGCTGCTGGGAGGCAGATTTAG

Protein sequence

LDDPLSSPSTNASALLLSQLIQKWLPVPCLSSISPPTPPLKPPYSLTPPPSKLATVLPPPPLPETTKIPHHSHVLQPNSGHRPTNLRRRLHSPFFNSLQYGRFLFAQYPTLGLAFEPLVPILGLYRSIPHSSFVAFFALYLGVVRNPSFSQYVRFNSMQAVTLDVLLVVPLLIQRILSPGRGGIGFRIMVWGHNGLFVFSVLCFVYSLVSCLLGRTPHLPLVADAAGRQI

Homology

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BLAST of Sgr016701 vs. NCBI nr
Match: XP_038879337.1 (protein TIC 20-II, chloroplastic [Benincasa hispida])

HSP 1 Score: 265.8 bits (678), Expect = 3.5e-67
Identity = 129/138 (93.48%), Postives = 135/138 (97.83%), Query Frame = 0


Query: 93  PFFNSLQYGRFLFAQYPTLGLAFEPLVPILGLYRSIPHSSFVAFFALYLGVVRNPSFSQY 152
           PFFNSLQYGRFLFAQYP LG AFEPL+PILGLYRSIPH+SFVAFFALYLG+VRNPSFS Y
Sbjct: 71  PFFNSLQYGRFLFAQYPALGFAFEPLLPILGLYRSIPHASFVAFFALYLGLVRNPSFSHY 130

Query: 153 VRFNSMQAVTLDVLLVVPLLIQRILSPGRGGIGFRIMVWGHNGLFVFSVLCFVYSLVSCL 212
           VRFNSMQAVTLDVLLVVPLLIQRILSPGRGG+GF+IMVWGHNGLFVFSVLCFVYSLVSCL
Sbjct: 131 VRFNSMQAVTLDVLLVVPLLIQRILSPGRGGLGFQIMVWGHNGLFVFSVLCFVYSLVSCL 190

Query: 213 LGRTPHLPLVADAAGRQI 231
           LGRTPHLP+VADAAGRQI
Sbjct: 191 LGRTPHLPIVADAAGRQI 208

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BLAST of Sgr016701 vs. NCBI nr
Match: XP_008443678.1 (PREDICTED: protein TIC 20-II, chloroplastic [Cucumis melo] >XP_008443680.1 PREDICTED: protein TIC 20-II, chloroplastic [Cucumis melo] >XP_008443681.1 PREDICTED: protein TIC 20-II, chloroplastic [Cucumis melo] >XP_008443682.1 PREDICTED: protein TIC 20-II, chloroplastic [Cucumis melo] >XP_008443683.1 PREDICTED: protein TIC 20-II, chloroplastic [Cucumis melo] >KAA0038239.1 protein TIC 20-II [Cucumis melo var. makuwa] >TYK30638.1 protein TIC 20-II [Cucumis melo var. makuwa])

HSP 1 Score: 264.2 bits (674), Expect = 1.0e-66
Identity = 134/156 (85.90%), Postives = 140/156 (89.74%), Query Frame = 0


Query: 81  HRPTNLRRRLHS------PFFNSLQYGRFLFAQYPTLGLAFEPLVPILGLYRSIPHSSFV 140
           + PT    RL S      PFFNSLQYGRFLFAQYP LG AFEPL+PILGLYRSIPHSSFV
Sbjct: 56  YNPTPATDRLISAVSYTLPFFNSLQYGRFLFAQYPALGFAFEPLLPILGLYRSIPHSSFV 115

Query: 141 AFFALYLGVVRNPSFSQYVRFNSMQAVTLDVLLVVPLLIQRILSPGRGGIGFRIMVWGHN 200
           AFFALYLGVVRNPSFS YVRFNSMQAVTLDVLLVVPLLIQRILSPGRGGIGF+IMVW HN
Sbjct: 116 AFFALYLGVVRNPSFSHYVRFNSMQAVTLDVLLVVPLLIQRILSPGRGGIGFKIMVWSHN 175

Query: 201 GLFVFSVLCFVYSLVSCLLGRTPHLPLVADAAGRQI 231
           GLFVFSVLCF+YS+VSCLLGRTPHLP+VADAAGRQI
Sbjct: 176 GLFVFSVLCFLYSMVSCLLGRTPHLPIVADAAGRQI 211

[Close]

BLAST of Sgr016701 vs. NCBI nr
Match: XP_004139099.1 (protein TIC 20-II, chloroplastic [Cucumis sativus] >XP_011660219.1 protein TIC 20-II, chloroplastic [Cucumis sativus] >XP_011660220.1 protein TIC 20-II, chloroplastic [Cucumis sativus] >KGN66660.1 hypothetical protein Csa_006938 [Cucumis sativus])

HSP 1 Score: 260.4 bits (664), Expect = 1.5e-65
Identity = 132/156 (84.62%), Postives = 139/156 (89.10%), Query Frame = 0


Query: 81  HRPTNLRRRLHS------PFFNSLQYGRFLFAQYPTLGLAFEPLVPILGLYRSIPHSSFV 140
           + PT    RL S      PFFNSLQYGRFLFAQYP LG AFEPL+PILGLYRSIPHSSFV
Sbjct: 53  YNPTPATDRLISAVAYTLPFFNSLQYGRFLFAQYPALGFAFEPLLPILGLYRSIPHSSFV 112

Query: 141 AFFALYLGVVRNPSFSQYVRFNSMQAVTLDVLLVVPLLIQRILSPGRGGIGFRIMVWGHN 200
           AFFALYLGVVRNPSF+ YVRFNSMQAVTLDVLLVVPLLIQRILSPGR GIGF+IMVW HN
Sbjct: 113 AFFALYLGVVRNPSFNHYVRFNSMQAVTLDVLLVVPLLIQRILSPGRSGIGFKIMVWSHN 172

Query: 201 GLFVFSVLCFVYSLVSCLLGRTPHLPLVADAAGRQI 231
           GLFVFSVLCF+YS+VSCLLGRTPHLP+VADAAGRQI
Sbjct: 173 GLFVFSVLCFLYSVVSCLLGRTPHLPIVADAAGRQI 208

[Close]

BLAST of Sgr016701 vs. NCBI nr
Match: XP_022149956.1 (protein TIC 20-II, chloroplastic-like [Momordica charantia])

HSP 1 Score: 258.8 bits (660), Expect = 4.3e-65
Identity = 131/156 (83.97%), Postives = 140/156 (89.74%), Query Frame = 0


Query: 81  HRPTNLRRRLHS------PFFNSLQYGRFLFAQYPTLGLAFEPLVPILGLYRSIPHSSFV 140
           + PT    RL S      PFFNSLQYGRFLFAQ+P LG+AF+PL+P+LGLYRSIPHS+FV
Sbjct: 43  YNPTPATDRLVSAVAYTLPFFNSLQYGRFLFAQHPALGVAFQPLLPVLGLYRSIPHSTFV 102

Query: 141 AFFALYLGVVRNPSFSQYVRFNSMQAVTLDVLLVVPLLIQRILSPGRGGIGFRIMVWGHN 200
           AFFALYLGVVRNP+FS YVRFNSMQAVTLDVLLVVPLLIQRILSPGR GIGF+IMVWGHN
Sbjct: 103 AFFALYLGVVRNPAFSHYVRFNSMQAVTLDVLLVVPLLIQRILSPGRAGIGFQIMVWGHN 162

Query: 201 GLFVFSVLCFVYSLVSCLLGRTPHLPLVADAAGRQI 231
           GLFVFSVLCFVYSLVSCLLGRTPHLPLVADAA RQI
Sbjct: 163 GLFVFSVLCFVYSLVSCLLGRTPHLPLVADAAARQI 198

[Close]

BLAST of Sgr016701 vs. NCBI nr
Match: XP_023005745.1 (protein TIC 20-II, chloroplastic-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 258.5 bits (659), Expect = 5.6e-65
Identity = 129/156 (82.69%), Postives = 139/156 (89.10%), Query Frame = 0


Query: 81  HRPTNLRRRLHS------PFFNSLQYGRFLFAQYPTLGLAFEPLVPILGLYRSIPHSSFV 140
           + PT    RL S      PFFNSLQYGRFLF QYP LGLAFEP++PILGLYRSIPHSSF+
Sbjct: 53  YNPTPATDRLISAVAYTLPFFNSLQYGRFLFTQYPVLGLAFEPILPILGLYRSIPHSSFI 112

Query: 141 AFFALYLGVVRNPSFSQYVRFNSMQAVTLDVLLVVPLLIQRILSPGRGGIGFRIMVWGHN 200
           AFFALYLGVVRNP+F++YVRFNSMQAVTLDVLL VPLLIQRILSPG GG+GF+IMVWGHN
Sbjct: 113 AFFALYLGVVRNPNFNRYVRFNSMQAVTLDVLLAVPLLIQRILSPGSGGLGFQIMVWGHN 172

Query: 201 GLFVFSVLCFVYSLVSCLLGRTPHLPLVADAAGRQI 231
           GLF+FSVLCFVYSL SCLLGRTPHLPLVADAAGRQI
Sbjct: 173 GLFLFSVLCFVYSLASCLLGRTPHLPLVADAAGRQI 208

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BLAST of Sgr016701 vs. ExPASy Swiss-Prot
Match: O82251 (Protein TIC 20-II, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC20-II PE=2 SV=1)

HSP 1 Score: 209.1 bits (531), Expect = 5.1e-53
Identity = 120/213 (56.34%), Postives = 145/213 (68.08%), Query Frame = 0


Query: 29  CLSSISPPTPPLKP----PYSLTPPPSKLATVLPPPPLPETTKIPHHSHVLQPNSGHRPT 88
           CLS     T PL      P SL+ P S   ++ P             +  L   + + PT
Sbjct: 5   CLSLHQTLTNPLSAPRCRPLSLSFPGSSTFSIRPS---------SRRATALTTRASYTPT 64

Query: 89  NLRRRLHS------PFFNSLQYGRFLFAQYPTLGLAFEPLVPILGLYRSIPHSSFVAFFA 148
               R+ S      PFFNSLQYGRFLFAQYP LGL FEP+ PIL LYRS+P++SFVAFF 
Sbjct: 65  PATERVISIASYALPFFNSLQYGRFLFAQYPRLGLLFEPIFPILNLYRSVPYASFVAFFG 124

Query: 149 LYLGVVRNPSFSQYVRFNSMQAVTLDVLLVVPLLIQRILSPGR-GGIGFRIMVWGHNGLF 208
           LYLGVVRN SFS+YVRFN+MQAVTLDVLL VP+L+ RIL PG+ GG G + M+WGH G+F
Sbjct: 125 LYLGVVRNTSFSRYVRFNAMQAVTLDVLLAVPVLLTRILDPGQGGGFGMKAMMWGHTGVF 184

Query: 209 VFSVLCFVYSLVSCLLGRTPHLPLVADAAGRQI 231
           VFS +CFVY +VS LLG+TP++P VADAAGRQ+
Sbjct: 185 VFSFMCFVYGVVSSLLGKTPYIPFVADAAGRQL 208

[Close]

BLAST of Sgr016701 vs. ExPASy Swiss-Prot
Match: Q9FM67 (Protein TIC 20-v, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC20-V PE=2 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 1.8e-26
Identity = 80/210 (38.10%), Postives = 117/210 (55.71%), Query Frame = 0


Query: 30  LSSISPPTPPLKPPYSLTPP---PSKLATVLPPPPLPETTKIPHHSHVLQPNSGHRPTNL 89
           +S    P P L    +LT     P  L T  P P L         + VLQ + G    + 
Sbjct: 4   ISQFFAPLPSLTGTLTLTGRSFLPLNLDTQFPKPRLSRDRAA---TLVLQ-SKGDDSVDA 63

Query: 90  RRRLHS------PFFNSLQYGRFLFAQYPTLGLAFEPLVPILGLYRSIPHSSFVAFFALY 149
             R+ S      PFF+ +QYG+F+  QY    +  +PL P +  ++S P + F+ F  LY
Sbjct: 64  SDRIISAVCYFYPFFDGIQYGKFIITQYQPFQILIQPLFPAIRAFKSFPFNGFLIFITLY 123

Query: 150 LGVVRNPSFSQYVRFNSMQAVTLDVLLVVPLLIQRILSPGRGGIGFRIMVWGHNGLFVFS 209
             VVRNP+FS+YVRFN+MQA+ LDVLL+ P L++R  +P R G G  +++   + +F+F 
Sbjct: 124 FVVVRNPNFSRYVRFNTMQAIVLDVLLIFPDLLERSFNP-RDGFGLDVVMSLDSTVFLFL 183

Query: 210 VLCFVYSLVSCLLGRTPHLPLVADAAGRQI 231
           ++  +Y   +CL G TP LPLVA+AA RQ+
Sbjct: 184 LVSLIYGFSACLFGLTPRLPLVAEAADRQV 208

[Close]

BLAST of Sgr016701 vs. ExPASy Swiss-Prot
Match: P73387 (Tic20 family protein OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=sll1737 PE=3 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 3.5e-17
Identity = 45/141 (31.91%), Postives = 84/141 (59.57%), Query Frame = 0


Query: 93  PFFNSLQYGRFLFAQYPTLGLAFEPLVPILGLYRSIPHSSFVAFFALYLGVVRNPSFSQY 152
           P  ++  +G FL  Q+P L + + P++P+L  Y   P +SF+ F  L++ VVRN + S +
Sbjct: 22  PLIDAFMFGGFLLQQFPVLQIIYLPIMPLLQFYYQFPFASFIIFIVLFMAVVRNNNISHF 81

Query: 153 VRFNSMQAVTLDVLL-VVPLLIQRILSP--GRGGIGFRIMVWGHNGLFVFSVLCFVYSLV 212
           +RFN+MQA+ + +LL +  L++  ++ P  G+G +   +    +N  F+ ++ C  + +V
Sbjct: 82  IRFNAMQAILIGILLSLFGLIVAYVIQPVFGQGLVTETL----YNFAFLGALACGFFGIV 141

Query: 213 SCLLGRTPHLPLVADAAGRQI 231
             +LGR   +P ++DAA  Q+
Sbjct: 142 QSVLGRYAEIPTISDAAYSQV 158

[Close]

BLAST of Sgr016701 vs. ExPASy TrEMBL
Match: A0A1S3B9C6 (Protein TIC 20 OS=Cucumis melo OX=3656 GN=LOC103487217 PE=3 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 5.0e-67
Identity = 134/156 (85.90%), Postives = 140/156 (89.74%), Query Frame = 0


Query: 81  HRPTNLRRRLHS------PFFNSLQYGRFLFAQYPTLGLAFEPLVPILGLYRSIPHSSFV 140
           + PT    RL S      PFFNSLQYGRFLFAQYP LG AFEPL+PILGLYRSIPHSSFV
Sbjct: 56  YNPTPATDRLISAVSYTLPFFNSLQYGRFLFAQYPALGFAFEPLLPILGLYRSIPHSSFV 115

Query: 141 AFFALYLGVVRNPSFSQYVRFNSMQAVTLDVLLVVPLLIQRILSPGRGGIGFRIMVWGHN 200
           AFFALYLGVVRNPSFS YVRFNSMQAVTLDVLLVVPLLIQRILSPGRGGIGF+IMVW HN
Sbjct: 116 AFFALYLGVVRNPSFSHYVRFNSMQAVTLDVLLVVPLLIQRILSPGRGGIGFKIMVWSHN 175

Query: 201 GLFVFSVLCFVYSLVSCLLGRTPHLPLVADAAGRQI 231
           GLFVFSVLCF+YS+VSCLLGRTPHLP+VADAAGRQI
Sbjct: 176 GLFVFSVLCFLYSMVSCLLGRTPHLPIVADAAGRQI 211

[Close]

BLAST of Sgr016701 vs. ExPASy TrEMBL
Match: A0A5D3E499 (Protein TIC 20 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold84664G00600 PE=3 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 5.0e-67
Identity = 134/156 (85.90%), Postives = 140/156 (89.74%), Query Frame = 0


Query: 81  HRPTNLRRRLHS------PFFNSLQYGRFLFAQYPTLGLAFEPLVPILGLYRSIPHSSFV 140
           + PT    RL S      PFFNSLQYGRFLFAQYP LG AFEPL+PILGLYRSIPHSSFV
Sbjct: 56  YNPTPATDRLISAVSYTLPFFNSLQYGRFLFAQYPALGFAFEPLLPILGLYRSIPHSSFV 115

Query: 141 AFFALYLGVVRNPSFSQYVRFNSMQAVTLDVLLVVPLLIQRILSPGRGGIGFRIMVWGHN 200
           AFFALYLGVVRNPSFS YVRFNSMQAVTLDVLLVVPLLIQRILSPGRGGIGF+IMVW HN
Sbjct: 116 AFFALYLGVVRNPSFSHYVRFNSMQAVTLDVLLVVPLLIQRILSPGRGGIGFKIMVWSHN 175

Query: 201 GLFVFSVLCFVYSLVSCLLGRTPHLPLVADAAGRQI 231
           GLFVFSVLCF+YS+VSCLLGRTPHLP+VADAAGRQI
Sbjct: 176 GLFVFSVLCFLYSMVSCLLGRTPHLPIVADAAGRQI 211

[Close]

BLAST of Sgr016701 vs. ExPASy TrEMBL
Match: A0A0A0LXS7 (Protein TIC 20 OS=Cucumis sativus OX=3659 GN=Csa_1G654890 PE=3 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 7.2e-66
Identity = 132/156 (84.62%), Postives = 139/156 (89.10%), Query Frame = 0


Query: 81  HRPTNLRRRLHS------PFFNSLQYGRFLFAQYPTLGLAFEPLVPILGLYRSIPHSSFV 140
           + PT    RL S      PFFNSLQYGRFLFAQYP LG AFEPL+PILGLYRSIPHSSFV
Sbjct: 53  YNPTPATDRLISAVAYTLPFFNSLQYGRFLFAQYPALGFAFEPLLPILGLYRSIPHSSFV 112

Query: 141 AFFALYLGVVRNPSFSQYVRFNSMQAVTLDVLLVVPLLIQRILSPGRGGIGFRIMVWGHN 200
           AFFALYLGVVRNPSF+ YVRFNSMQAVTLDVLLVVPLLIQRILSPGR GIGF+IMVW HN
Sbjct: 113 AFFALYLGVVRNPSFNHYVRFNSMQAVTLDVLLVVPLLIQRILSPGRSGIGFKIMVWSHN 172

Query: 201 GLFVFSVLCFVYSLVSCLLGRTPHLPLVADAAGRQI 231
           GLFVFSVLCF+YS+VSCLLGRTPHLP+VADAAGRQI
Sbjct: 173 GLFVFSVLCFLYSVVSCLLGRTPHLPIVADAAGRQI 208

[Close]

BLAST of Sgr016701 vs. ExPASy TrEMBL
Match: A0A6J1DA02 (Protein TIC 20 OS=Momordica charantia OX=3673 GN=LOC111018249 PE=3 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 2.1e-65
Identity = 131/156 (83.97%), Postives = 140/156 (89.74%), Query Frame = 0


Query: 81  HRPTNLRRRLHS------PFFNSLQYGRFLFAQYPTLGLAFEPLVPILGLYRSIPHSSFV 140
           + PT    RL S      PFFNSLQYGRFLFAQ+P LG+AF+PL+P+LGLYRSIPHS+FV
Sbjct: 43  YNPTPATDRLVSAVAYTLPFFNSLQYGRFLFAQHPALGVAFQPLLPVLGLYRSIPHSTFV 102

Query: 141 AFFALYLGVVRNPSFSQYVRFNSMQAVTLDVLLVVPLLIQRILSPGRGGIGFRIMVWGHN 200
           AFFALYLGVVRNP+FS YVRFNSMQAVTLDVLLVVPLLIQRILSPGR GIGF+IMVWGHN
Sbjct: 103 AFFALYLGVVRNPAFSHYVRFNSMQAVTLDVLLVVPLLIQRILSPGRAGIGFQIMVWGHN 162

Query: 201 GLFVFSVLCFVYSLVSCLLGRTPHLPLVADAAGRQI 231
           GLFVFSVLCFVYSLVSCLLGRTPHLPLVADAA RQI
Sbjct: 163 GLFVFSVLCFVYSLVSCLLGRTPHLPLVADAAARQI 198

[Close]

BLAST of Sgr016701 vs. ExPASy TrEMBL
Match: A0A6J1KY86 (Protein TIC 20 OS=Cucurbita maxima OX=3661 GN=LOC111498634 PE=3 SV=1)

HSP 1 Score: 258.5 bits (659), Expect = 2.7e-65
Identity = 129/156 (82.69%), Postives = 139/156 (89.10%), Query Frame = 0


Query: 81  HRPTNLRRRLHS------PFFNSLQYGRFLFAQYPTLGLAFEPLVPILGLYRSIPHSSFV 140
           + PT    RL S      PFFNSLQYGRFLF QYP LGLAFEP++PILGLYRSIPHSSF+
Sbjct: 60  YNPTPATDRLISAVAYTLPFFNSLQYGRFLFTQYPVLGLAFEPILPILGLYRSIPHSSFI 119

Query: 141 AFFALYLGVVRNPSFSQYVRFNSMQAVTLDVLLVVPLLIQRILSPGRGGIGFRIMVWGHN 200
           AFFALYLGVVRNP+F++YVRFNSMQAVTLDVLL VPLLIQRILSPG GG+GF+IMVWGHN
Sbjct: 120 AFFALYLGVVRNPNFNRYVRFNSMQAVTLDVLLAVPLLIQRILSPGSGGLGFQIMVWGHN 179

Query: 201 GLFVFSVLCFVYSLVSCLLGRTPHLPLVADAAGRQI 231
           GLF+FSVLCFVYSL SCLLGRTPHLPLVADAAGRQI
Sbjct: 180 GLFLFSVLCFVYSLASCLLGRTPHLPLVADAAGRQI 215

[Close]

BLAST of Sgr016701 vs. TAIR 10
Match: AT2G47840.1 (Uncharacterised conserved protein ycf60 )

HSP 1 Score: 209.1 bits (531), Expect = 3.7e-54
Identity = 120/213 (56.34%), Postives = 145/213 (68.08%), Query Frame = 0


Query: 29  CLSSISPPTPPLKP----PYSLTPPPSKLATVLPPPPLPETTKIPHHSHVLQPNSGHRPT 88
           CLS     T PL      P SL+ P S   ++ P             +  L   + + PT
Sbjct: 5   CLSLHQTLTNPLSAPRCRPLSLSFPGSSTFSIRPS---------SRRATALTTRASYTPT 64

Query: 89  NLRRRLHS------PFFNSLQYGRFLFAQYPTLGLAFEPLVPILGLYRSIPHSSFVAFFA 148
               R+ S      PFFNSLQYGRFLFAQYP LGL FEP+ PIL LYRS+P++SFVAFF 
Sbjct: 65  PATERVISIASYALPFFNSLQYGRFLFAQYPRLGLLFEPIFPILNLYRSVPYASFVAFFG 124

Query: 149 LYLGVVRNPSFSQYVRFNSMQAVTLDVLLVVPLLIQRILSPGR-GGIGFRIMVWGHNGLF 208
           LYLGVVRN SFS+YVRFN+MQAVTLDVLL VP+L+ RIL PG+ GG G + M+WGH G+F
Sbjct: 125 LYLGVVRNTSFSRYVRFNAMQAVTLDVLLAVPVLLTRILDPGQGGGFGMKAMMWGHTGVF 184

Query: 209 VFSVLCFVYSLVSCLLGRTPHLPLVADAAGRQI 231
           VFS +CFVY +VS LLG+TP++P VADAAGRQ+
Sbjct: 185 VFSFMCFVYGVVSSLLGKTPYIPFVADAAGRQL 208

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BLAST of Sgr016701 vs. TAIR 10
Match: AT5G55710.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast thylakoid membrane, chloroplast; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 14 growth stages; BEST Arabidopsis thaliana protein match is: Uncharacterised conserved protein ycf60 (TAIR:AT2G47840.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 120.9 bits (302), Expect = 1.3e-27
Identity = 80/210 (38.10%), Postives = 117/210 (55.71%), Query Frame = 0


Query: 30  LSSISPPTPPLKPPYSLTPP---PSKLATVLPPPPLPETTKIPHHSHVLQPNSGHRPTNL 89
           +S    P P L    +LT     P  L T  P P L         + VLQ + G    + 
Sbjct: 4   ISQFFAPLPSLTGTLTLTGRSFLPLNLDTQFPKPRLSRDRAA---TLVLQ-SKGDDSVDA 63

Query: 90  RRRLHS------PFFNSLQYGRFLFAQYPTLGLAFEPLVPILGLYRSIPHSSFVAFFALY 149
             R+ S      PFF+ +QYG+F+  QY    +  +PL P +  ++S P + F+ F  LY
Sbjct: 64  SDRIISAVCYFYPFFDGIQYGKFIITQYQPFQILIQPLFPAIRAFKSFPFNGFLIFITLY 123

Query: 150 LGVVRNPSFSQYVRFNSMQAVTLDVLLVVPLLIQRILSPGRGGIGFRIMVWGHNGLFVFS 209
             VVRNP+FS+YVRFN+MQA+ LDVLL+ P L++R  +P R G G  +++   + +F+F 
Sbjct: 124 FVVVRNPNFSRYVRFNTMQAIVLDVLLIFPDLLERSFNP-RDGFGLDVVMSLDSTVFLFL 183

Query: 210 VLCFVYSLVSCLLGRTPHLPLVADAAGRQI 231
           ++  +Y   +CL G TP LPLVA+AA RQ+
Sbjct: 184 LVSLIYGFSACLFGLTPRLPLVAEAADRQV 208

The following BLAST results are available for this feature:

BLAST of Sgr016701 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038879337.1	3.5e-67	93.48	protein TIC 20-II, chloroplastic [Benincasa hispida]	[more]
XP_008443678.1	1.0e-66	85.90	PREDICTED: protein TIC 20-II, chloroplastic [Cucumis melo] >XP_008443680.1 PREDI...	[more]
XP_004139099.1	1.5e-65	84.62	protein TIC 20-II, chloroplastic [Cucumis sativus] >XP_011660219.1 protein TIC 2...	[more]
XP_022149956.1	4.3e-65	83.97	protein TIC 20-II, chloroplastic-like [Momordica charantia]	[more]
XP_023005745.1	5.6e-65	82.69	protein TIC 20-II, chloroplastic-like isoform X2 [Cucurbita maxima]	[more]

BLAST of Sgr016701 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O82251	5.1e-53	56.34	Protein TIC 20-II, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC20-II PE=...	[more]
Q9FM67	1.8e-26	38.10	Protein TIC 20-v, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC20-V PE=2 ...	[more]
P73387	3.5e-17	31.91	Tic20 family protein OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 ...	[more]

BLAST of Sgr016701 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3B9C6	5.0e-67	85.90	Protein TIC 20 OS=Cucumis melo OX=3656 GN=LOC103487217 PE=3 SV=1	[more]
A0A5D3E499	5.0e-67	85.90	Protein TIC 20 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold84664G006...	[more]
A0A0A0LXS7	7.2e-66	84.62	Protein TIC 20 OS=Cucumis sativus OX=3659 GN=Csa_1G654890 PE=3 SV=1	[more]
A0A6J1DA02	2.1e-65	83.97	Protein TIC 20 OS=Momordica charantia OX=3673 GN=LOC111018249 PE=3 SV=1	[more]
A0A6J1KY86	2.7e-65	82.69	Protein TIC 20 OS=Cucurbita maxima OX=3661 GN=LOC111498634 PE=3 SV=1	[more]

BLAST of Sgr016701 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G47840.1	3.7e-54	56.34	Uncharacterised conserved protein ycf60	[more]
AT5G55710.1	1.3e-27	38.10	FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow...	[more]

InterPro

Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005691	Chloroplast protein import component Tic20	PFAM	PF16166	TIC20	coord: 93..226 e-value: 2.8E-39 score: 134.7
IPR005691	Chloroplast protein import component Tic20	PANTHER	PTHR33510	PROTEIN TIC 20-II, CHLOROPLASTIC	coord: 93..230
None	No IPR available	PANTHER	PTHR33510:SF5	PROTEIN TIC 20-II, CHLOROPLASTIC	coord: 93..230

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sgr016701.1	Sgr016701.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0009706	chloroplast inner membrane
cellular_component	GO:0016021	integral component of membrane