Sgr016616 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr016616 vs. NCBI nr
Match: XP_038895790.1 (putative E3 ubiquitin-protein ligase RING1a [Benincasa hispida])

HSP 1 Score: 536.2 bits (1380), Expect = 2.3e-148
Identity = 299/395 (75.70%), Postives = 326/395 (82.53%), Query Frame = 0

Query: 1   MVPEGLTASASNPEPHTIESKPLEVGNGAESTFSPRFKSAAALAGWDEEALLIASLVVDD 60
           MVPEG TASAS PE  TIESKP  +GN  ES  SPRFKSAAA+AGWDEE LLIASLVVDD
Sbjct: 1   MVPEGSTASASEPERRTIESKPPGIGNDVESFLSPRFKSAAAMAGWDEETLLIASLVVDD 60

Query: 61  TPERGFRHKKRSVLQCKSPPSGSRRKRRTSTSLISSPVAVLNLDEEETTVKDDPPKQDPE 120
           TPER  + KKRSVLQ KSP SG RRKRRT TS+IS PV VL+LDEEE TVKDD PKQDPE
Sbjct: 61  TPERELQQKKRSVLQRKSPASGLRRKRRTLTSIISVPVTVLDLDEEEPTVKDDAPKQDPE 120

Query: 121 ISVPETKKSDKLGEQNAGA-SFSCSALPCMEKLREELSCAICLEICFEPSTTPCGHSFCK 180
            +  E KKSD + EQ   A S SCS LPCM+KLREELSCAICLEICFEPSTTPCGHSFCK
Sbjct: 121 TAEAEPKKSDSMVEQKVDASSLSCSTLPCMDKLREELSCAICLEICFEPSTTPCGHSFCK 180

Query: 181 KCLRSAADKCGKRCPKCRQLISNGRSCTVNTVLWNTIQLLFPQEVDARKEAKGCSRSQEK 240
           KCLRSAADKCGKRCPKCRQL+SNGRSCTVNTVLWNTIQLLFPQEV+ARK+AK C+  ++K
Sbjct: 181 KCLRSAADKCGKRCPKCRQLMSNGRSCTVNTVLWNTIQLLFPQEVEARKKAKECNSHEKK 240

Query: 241 IHNPEKAFY-------TRDTGTSSRH----GFTRRGGETTIRDEDGEQDSGMIQRAVSSN 300
           I +PEKAFY       TR  GT+SRH      +RR GE T+R+E GE+DS M+ RA S N
Sbjct: 241 IEDPEKAFYSSLQNYNTRAIGTASRHVVSSSSSRRRGEITVREEYGERDSRMMLRAASLN 300

Query: 301 AESRVQSRMRRRSIRPVRMTTTRDVDVRSSSRRGTPDQDQDAALALRLQREEFMEAFRDT 360
           AESRVQSRM RRSIRP+RM  TRD D R SSRRGTPDQD+DAALALRLQREEF+EAFRDT
Sbjct: 301 AESRVQSRMLRRSIRPIRM-ATRDADAR-SSRRGTPDQDRDAALALRLQREEFLEAFRDT 360

Query: 361 TQVQTRSSLSLARANLRAMASRAAINLRINGRRNL 384
           TQVQTRSS SL RANLRA+ASRAAINLRI+G +NL
Sbjct: 361 TQVQTRSSTSLTRANLRAIASRAAINLRISGHQNL 393

BLAST of Sgr016616 vs. NCBI nr
Match: XP_004145719.1 (uncharacterized protein LOC101209296 [Cucumis sativus] >KGN58207.1 hypothetical protein Csa_017490 [Cucumis sativus])

HSP 1 Score: 532.7 bits (1371), Expect = 2.6e-147
Identity = 299/391 (76.47%), Postives = 328/391 (83.89%), Query Frame = 0

Query: 1   MVPEGLTASASNPEPHTIESKPLEVGNGAESTFSPRFKSAAALAGWDEEALLIASLVVDD 60
           MV EG TASAS       ESKPL + N A+S  SPRFKSAAA+AGWDEEALLIASLVVDD
Sbjct: 1   MVSEGSTASAS-------ESKPLGIANDADSFLSPRFKSAAAMAGWDEEALLIASLVVDD 60

Query: 61  TPERGFRHKKRSVLQCKSPPSGSRRKRRTSTSLISSPVAVLNLDEEETTVKDDPPKQDPE 120
           TPER F+ KKRSVLQ KSP SGSRRKRRT TS+IS PVAVL+LDE E TVKDD PKQ+P+
Sbjct: 61  TPEREFQQKKRSVLQRKSPASGSRRKRRTLTSIISIPVAVLDLDETEPTVKDDGPKQEPK 120

Query: 121 ISVPETKKSDKLGEQNAGA-SFSCSALPCMEKLREELSCAICLEICFEPSTTPCGHSFCK 180
            +  E KKSD + EQ A A S + S LPCM+KLREELSCAICLEICFEPSTTPCGHSFCK
Sbjct: 121 SAEAEAKKSDSMVEQKADASSLTSSTLPCMDKLREELSCAICLEICFEPSTTPCGHSFCK 180

Query: 181 KCLRSAADKCGKRCPKCRQLISNGRSCTVNTVLWNTIQLLFPQEVDARKEAKGCSRSQEK 240
           KCLRSAADKCGKRCPKCRQLISNGRSCTVNTVLWNTIQLLFP+EV+ARKEAK C+  ++K
Sbjct: 181 KCLRSAADKCGKRCPKCRQLISNGRSCTVNTVLWNTIQLLFPKEVEARKEAKECNSREKK 240

Query: 241 IHNPEKAFY-------TRDTGTSSRHGFTRRGGETTIRDEDGEQDSGMIQRAVSSNAESR 300
           I +PEKAFY       TR  GTSSRH  TRR GE T+++E+GE DS + QRAVSSNAESR
Sbjct: 241 IQDPEKAFYSSLQNYDTRPIGTSSRHASTRRRGEITVQEENGEWDSRVTQRAVSSNAESR 300

Query: 301 VQSRMRRRSIRPVRMTTTRDVDVRSSSRRGTPDQDQDAALALRLQREEFMEAFRDTTQVQ 360
           VQSRM RRSIRP+RM  TR VDVR SSRRGTPDQDQDAALALRLQREEF+EAFRDTTQV 
Sbjct: 301 VQSRMHRRSIRPMRM-ATRGVDVR-SSRRGTPDQDQDAALALRLQREEFLEAFRDTTQVH 360

Query: 361 TRSSLSLARANLRAMASRAAINLRINGRRNL 384
           TRSSLSL RAN+RA+ASRAAINLRI+G +NL
Sbjct: 361 TRSSLSLTRANIRAIASRAAINLRISGHQNL 382

BLAST of Sgr016616 vs. NCBI nr
Match: KAG7017808.1 (Tripartite motif-containing protein 65, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 515.0 bits (1325), Expect = 5.6e-142
Identity = 293/389 (75.32%), Postives = 324/389 (83.29%), Query Frame = 0

Query: 1   MVPEGLTASASNPEPHTIESKPLEVGNGAESTFSPRFKSAAALAGWDEEALLIASLVVDD 60
           MV EG TASAS PE   IES+PLE GN A+S FSPRFKSAAA+AGWDEEALLIASLVVDD
Sbjct: 1   MVQEGSTASASEPERRAIESRPLESGNDADSFFSPRFKSAAAMAGWDEEALLIASLVVDD 60

Query: 61  TPERGFRHKKRSVLQCKSPPSGSRRKRRTSTSLISSPVAVLNLDEEETTVKDDPPKQDPE 120
           TPER F+ KKRSVL CKSP SGSRRKRRTSTS+IS PVAVL+LD EE TV+DD  KQDPE
Sbjct: 61  TPEREFQQKKRSVLHCKSPTSGSRRKRRTSTSIISIPVAVLDLDAEEPTVRDDATKQDPE 120

Query: 121 ISVPETKKSDKLGEQNAG-ASFSCSALPCMEKLREELSCAICLEICFEPSTTPCGHSFCK 180
            +  E KKS+ L EQ AG +S SCS LPC++KLREELSCAICLEICFEPSTTPCGHSFCK
Sbjct: 121 TAEAEPKKSNSLIEQKAGDSSLSCSTLPCIDKLREELSCAICLEICFEPSTTPCGHSFCK 180

Query: 181 KCLRSAADKCGKRCPKCRQLISNGRSCTVNTVLWNTIQLLFPQEVDARK-EAKGCSRSQE 240
           KCLRSAAD+CGKRCPKCRQLISNGRSCTVNTVLWNTIQLLFPQEV+ARK  AK CS  ++
Sbjct: 181 KCLRSAADRCGKRCPKCRQLISNGRSCTVNTVLWNTIQLLFPQEVEARKVVAKECSSPEK 240

Query: 241 KIHNPEKAFY-------TRDTGTSSRHG-FTRRGGETTIR-DEDGEQDSGMIQRAVSSNA 300
           K+ +PEKAFY       T+ T TSSR G  +RR GE T+R DEDGE+D+ ++Q+A SSNA
Sbjct: 241 KVQDPEKAFYTSLQNHNTQATDTSSRRGSSSRRRGEITVRDDEDGERDNRVMQQAASSNA 300

Query: 301 ESRVQSRMRRRSIRPVRMTTTRDVDVRSSSRRGTPDQDQDAALALRLQREEFMEAFRDTT 360
           ESRV SRM+RRSIR +R+  TRD DVR SSRRGTPDQDQDAALALRLQREEFMEAFRDT 
Sbjct: 301 ESRVPSRMQRRSIRSMRI-ATRDSDVR-SSRRGTPDQDQDAALALRLQREEFMEAFRDTP 360

Query: 361 QVQTRSSLSLARANLRAMASRAAINLRIN 379
               RSSLSLARANLRAMASRAA++   N
Sbjct: 361 VQTRRSSLSLARANLRAMASRAAVSRHRN 387

BLAST of Sgr016616 vs. NCBI nr
Match: KAG6581080.1 (Tripartite motif-containing protein 65, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 515.0 bits (1325), Expect = 5.6e-142
Identity = 293/389 (75.32%), Postives = 324/389 (83.29%), Query Frame = 0

Query: 1   MVPEGLTASASNPEPHTIESKPLEVGNGAESTFSPRFKSAAALAGWDEEALLIASLVVDD 60
           MV EG TASAS PE   IES+PLE GN A+S FSPRFKSAAA+AGWDEEALLIASLVVDD
Sbjct: 1   MVQEGSTASASEPERRAIESRPLESGNDADSFFSPRFKSAAAMAGWDEEALLIASLVVDD 60

Query: 61  TPERGFRHKKRSVLQCKSPPSGSRRKRRTSTSLISSPVAVLNLDEEETTVKDDPPKQDPE 120
           TPER F+ KKRSVL CKSP SGSRRKRRTSTS+IS PVAVL+LD EE TV+DD  KQDPE
Sbjct: 61  TPEREFQQKKRSVLHCKSPTSGSRRKRRTSTSIISIPVAVLDLDAEEPTVRDDATKQDPE 120

Query: 121 ISVPETKKSDKLGEQNAG-ASFSCSALPCMEKLREELSCAICLEICFEPSTTPCGHSFCK 180
            +  E KKS+ L EQ AG +S SCS LPC++KLREELSCAICLEICFEPSTTPCGHSFCK
Sbjct: 121 TAEAEPKKSNSLIEQKAGDSSLSCSTLPCIDKLREELSCAICLEICFEPSTTPCGHSFCK 180

Query: 181 KCLRSAADKCGKRCPKCRQLISNGRSCTVNTVLWNTIQLLFPQEVDARK-EAKGCSRSQE 240
           KCLRSAAD+CGKRCPKCRQLISNGRSCTVNTVLWNTIQLLFPQEV+ARK  AK CS  ++
Sbjct: 181 KCLRSAADRCGKRCPKCRQLISNGRSCTVNTVLWNTIQLLFPQEVEARKVVAKECSSPEK 240

Query: 241 KIHNPEKAFY-------TRDTGTSSRHG-FTRRGGETTIR-DEDGEQDSGMIQRAVSSNA 300
           K+ +PEKAFY       T+ T TSSR G  +RR GE T+R DEDGE+D+ ++Q+A SSNA
Sbjct: 241 KVQDPEKAFYTSLQNHNTQATDTSSRRGSSSRRRGEITVRDDEDGERDNRVMQQAASSNA 300

Query: 301 ESRVQSRMRRRSIRPVRMTTTRDVDVRSSSRRGTPDQDQDAALALRLQREEFMEAFRDTT 360
           ESRV SRM+RRSIR +R+  TRD DVR SSRRGTPDQDQDAALALRLQREEFMEAFRDT 
Sbjct: 301 ESRVPSRMQRRSIRSMRI-ATRDSDVR-SSRRGTPDQDQDAALALRLQREEFMEAFRDTP 360

Query: 361 QVQTRSSLSLARANLRAMASRAAINLRIN 379
               RSSLSLARANLRAMASRAA++   N
Sbjct: 361 VQPRRSSLSLARANLRAMASRAAVSRHRN 387

BLAST of Sgr016616 vs. NCBI nr
Match: XP_022934994.1 (E3 ubiquitin-protein ligase ORTHRUS 1-like [Cucurbita moschata] >XP_022934995.1 E3 ubiquitin-protein ligase ORTHRUS 1-like [Cucurbita moschata])

HSP 1 Score: 509.6 bits (1311), Expect = 2.3e-140
Identity = 290/389 (74.55%), Postives = 320/389 (82.26%), Query Frame = 0

Query: 1   MVPEGLTASASNPEPHTIESKPLEVGNGAESTFSPRFKSAAALAGWDEEALLIASLVVDD 60
           MV EG  ASAS PE   IES+PLE GN A+S FSPRFKSAAA+AGWDEEALLIASLVVDD
Sbjct: 1   MVQEGSRASASEPERRAIESRPLESGNDADSFFSPRFKSAAAMAGWDEEALLIASLVVDD 60

Query: 61  TPERGFRHKKRSVLQCKSPPSGSRRKRRTSTSLISSPVAVLNLDEEETTVKDDPPKQDPE 120
           TPER F+ KKRSVL CKSP SGSRRKRRTSTS+IS PVAVL+LD EE TV+DD  KQDPE
Sbjct: 61  TPEREFQQKKRSVLHCKSPTSGSRRKRRTSTSIISIPVAVLDLDAEEPTVRDDATKQDPE 120

Query: 121 ISVPETKKSDKLGEQNAG-ASFSCSALPCMEKLREELSCAICLEICFEPSTTPCGHSFCK 180
            +  E KKS+ L EQ AG +S SCS LPC++KLREELSCAICLEICFEPSTTPCGHSFCK
Sbjct: 121 TAEAEPKKSNSLIEQKAGDSSLSCSTLPCIDKLREELSCAICLEICFEPSTTPCGHSFCK 180

Query: 181 KCLRSAADKCGKRCPKCRQLISNGRSCTVNTVLWNTIQLLFPQEVDARK-EAKGCSRSQE 240
           KCLRSAAD+CGKRCPKCRQLISNGRSCTVNTVLWNTIQLLFPQEV+ARK  AK CS  ++
Sbjct: 181 KCLRSAADRCGKRCPKCRQLISNGRSCTVNTVLWNTIQLLFPQEVEARKVVAKECSSHEK 240

Query: 241 KIHNPEKAFY-------TRDTGTSSRHG-FTRRGGETTIR-DEDGEQDSGMIQRAVSSNA 300
           K+ +PE AFY       T+ T TSSR G  +RR GE T+R DEDGE+D+ ++Q+A S NA
Sbjct: 241 KVQDPENAFYSSLQNHNTQATDTSSRRGSSSRRRGEITVRDDEDGERDNRVMQQAASLNA 300

Query: 301 ESRVQSRMRRRSIRPVRMTTTRDVDVRSSSRRGTPDQDQDAALALRLQREEFMEAFRDTT 360
           ESRV SRM R+SIR +RM TTRD DV  SSRRGTPDQDQDAALALRLQREEFMEAFRDT 
Sbjct: 301 ESRVPSRMHRKSIRSMRM-TTRDADV--SSRRGTPDQDQDAALALRLQREEFMEAFRDTP 360

Query: 361 QVQTRSSLSLARANLRAMASRAAINLRIN 379
               RSSLSLARANLRAMASRAA++   N
Sbjct: 361 VQTRRSSLSLARANLRAMASRAAVSRHQN 386

BLAST of Sgr016616 vs. ExPASy Swiss-Prot
Match: Q6PJ69 (Tripartite motif-containing protein 65 OS=Homo sapiens OX=9606 GN=TRIM65 PE=1 SV=3)

HSP 1 Score: 69.3 bits (168), Expect = 1.1e-10
Identity = 29/68 (42.65%), Postives = 43/68 (63.24%), Query Frame = 0

Query: 152 LREELSCAICLEICFEPSTTPCGHSFCKKCLRSAADKCGKRCPKCRQLISNGRSCTVNTV 211
           L E+L+CAICL +  +P T PCGH+FC  C+R   D+CGK CP+CR+   +G     N  
Sbjct: 6   LEEKLTCAICLGLYQDPVTLPCGHNFCGACIRDWWDRCGKACPECREPFPDGAELRRNVA 65

Query: 212 LWNTIQLL 220
           L   ++++
Sbjct: 66  LSGVLEVV 73

BLAST of Sgr016616 vs. ExPASy Swiss-Prot
Match: Q7T308 (E3 ubiquitin-protein ligase rnf168 OS=Danio rerio OX=7955 GN=rnf168 PE=2 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 2.4e-10
Identity = 38/98 (38.78%), Postives = 50/98 (51.02%), Query Frame = 0

Query: 153 REELSCAICLEICFEPSTTPCGHSFCKKCLRSAADKCGKRCPKCRQLIS-----NGRSCT 212
           R +  C +CLEI  EP T PC H+FCK C     DK    CP CR+ +S     N R+ T
Sbjct: 21  RSDCVCPVCLEIFLEPVTLPCMHTFCKPCFLETVDKSNMCCPLCRKRVSTWARLNSRNKT 80

Query: 213 -VNTVLWNTIQLLFPQEVDARKEAKGCSRSQEKIHNPE 245
            VN  LW  IQ  FP + + R   +G    +E +  P+
Sbjct: 81  LVNMELWRRIQDAFPSQCERR--VQGIDDDEEAVMIPK 116

BLAST of Sgr016616 vs. ExPASy Swiss-Prot
Match: Q496Y0 (LON peptidase N-terminal domain and RING finger protein 3 OS=Homo sapiens OX=9606 GN=LONRF3 PE=1 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 7.6e-09
Identity = 43/128 (33.59%), Postives = 62/128 (48.44%), Query Frame = 0

Query: 105 EEETTVKDDPPKQDPEISVPETKKSDKLGEQNAGASFSCSALPCMEKLREELSCAICLEI 164
           +EET + +   KQD     P T + DK       ASF  S          +L CA+C+ +
Sbjct: 429 QEETGMPNKASKQD-----PPTDQGDKPALSLPLASFDAS----------DLECALCMRL 488

Query: 165 CFEPSTTPCGHSFCKKCLRSAADKCGKRCPKCRQLISN---GRSCTVNTVLWNTIQLLFP 224
            +EP TTPCGH+FC KCL    D   K CP C+  +S     R  + N ++   I    P
Sbjct: 489 FYEPVTTPCGHTFCLKCLERCLDHNAK-CPLCKDGLSQCLASRKYSKNVIMEELIAKFLP 540

Query: 225 QEVDARKE 230
           +E+  R++
Sbjct: 549 EELKERRK 540

BLAST of Sgr016616 vs. ExPASy Swiss-Prot
Match: Q8HXH0 (LON peptidase N-terminal domain and RING finger protein 3 OS=Macaca fascicularis OX=9541 GN=LONRF3 PE=2 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 1.7e-08
Identity = 42/128 (32.81%), Postives = 62/128 (48.44%), Query Frame = 0

Query: 105 EEETTVKDDPPKQDPEISVPETKKSDKLGEQNAGASFSCSALPCMEKLREELSCAICLEI 164
           +E+T + +   KQD     P T + DK       ASF  S          +L CA+C+ +
Sbjct: 388 QEDTGMPNKASKQD-----PPTDQGDKPALSLPLASFDAS----------DLECALCMRL 447

Query: 165 CFEPSTTPCGHSFCKKCLRSAADKCGKRCPKCRQLISN---GRSCTVNTVLWNTIQLLFP 224
            +EP TTPCGH+FC KCL    D   K CP C+  +S     R  + N ++   I    P
Sbjct: 448 FYEPVTTPCGHTFCLKCLERCLDHNAK-CPLCKDGLSQCLASRKYSKNVIMEELIAKFLP 499

Query: 225 QEVDARKE 230
           +E+  R++
Sbjct: 508 EELKERRK 499

BLAST of Sgr016616 vs. ExPASy Swiss-Prot
Match: Q92021 (Nuclear factor 7, brain OS=Xenopus laevis OX=8355 PE=1 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 3.8e-08
Identity = 52/163 (31.90%), Postives = 82/163 (50.31%), Query Frame = 0

Query: 104 DEEETTVKDDPPKQDPE---ISVPETK-KSDKLGEQNAGASFSCSALPCMEKLREELSCA 163
           D+E T   + P  + P+   I  PE + K  K+ E++  AS + S+L       EEL+C 
Sbjct: 89  DQEVTDTAEQPDSKTPQKRKIEEPEPEPKKAKVEEKD--ASKNASSLGAAGDFAEELTCP 148

Query: 164 ICLEICFEPSTTPCGHSFCKKCLRSAAD-KCGKRCPKCRQLISNGRSCTVNTVLWNTIQL 223
           +C+E+  +P    CGH+FC+ C+  A + +    CP+CR+ I++ R  T+N VL N  + 
Sbjct: 149 LCVELFKDPVMVACGHNFCRSCIDKAWEGQSSFACPECRESITD-RKYTINRVLANLAKK 208

Query: 224 -----LFPQEVDARKEAKGCSRSQEKIHNPEKAFYTRDTGTSS 257
                + P E   R   K CS   E++       Y +D GT S
Sbjct: 209 AACTPVTPVEKKTRPLEK-CSEHDERL-----KLYCKDDGTLS 242

BLAST of Sgr016616 vs. ExPASy TrEMBL
Match: A0A0A0LBY2 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G592120 PE=4 SV=1)

HSP 1 Score: 532.7 bits (1371), Expect = 1.2e-147
Identity = 299/391 (76.47%), Postives = 328/391 (83.89%), Query Frame = 0

Query: 1   MVPEGLTASASNPEPHTIESKPLEVGNGAESTFSPRFKSAAALAGWDEEALLIASLVVDD 60
           MV EG TASAS       ESKPL + N A+S  SPRFKSAAA+AGWDEEALLIASLVVDD
Sbjct: 1   MVSEGSTASAS-------ESKPLGIANDADSFLSPRFKSAAAMAGWDEEALLIASLVVDD 60

Query: 61  TPERGFRHKKRSVLQCKSPPSGSRRKRRTSTSLISSPVAVLNLDEEETTVKDDPPKQDPE 120
           TPER F+ KKRSVLQ KSP SGSRRKRRT TS+IS PVAVL+LDE E TVKDD PKQ+P+
Sbjct: 61  TPEREFQQKKRSVLQRKSPASGSRRKRRTLTSIISIPVAVLDLDETEPTVKDDGPKQEPK 120

Query: 121 ISVPETKKSDKLGEQNAGA-SFSCSALPCMEKLREELSCAICLEICFEPSTTPCGHSFCK 180
            +  E KKSD + EQ A A S + S LPCM+KLREELSCAICLEICFEPSTTPCGHSFCK
Sbjct: 121 SAEAEAKKSDSMVEQKADASSLTSSTLPCMDKLREELSCAICLEICFEPSTTPCGHSFCK 180

Query: 181 KCLRSAADKCGKRCPKCRQLISNGRSCTVNTVLWNTIQLLFPQEVDARKEAKGCSRSQEK 240
           KCLRSAADKCGKRCPKCRQLISNGRSCTVNTVLWNTIQLLFP+EV+ARKEAK C+  ++K
Sbjct: 181 KCLRSAADKCGKRCPKCRQLISNGRSCTVNTVLWNTIQLLFPKEVEARKEAKECNSREKK 240

Query: 241 IHNPEKAFY-------TRDTGTSSRHGFTRRGGETTIRDEDGEQDSGMIQRAVSSNAESR 300
           I +PEKAFY       TR  GTSSRH  TRR GE T+++E+GE DS + QRAVSSNAESR
Sbjct: 241 IQDPEKAFYSSLQNYDTRPIGTSSRHASTRRRGEITVQEENGEWDSRVTQRAVSSNAESR 300

Query: 301 VQSRMRRRSIRPVRMTTTRDVDVRSSSRRGTPDQDQDAALALRLQREEFMEAFRDTTQVQ 360
           VQSRM RRSIRP+RM  TR VDVR SSRRGTPDQDQDAALALRLQREEF+EAFRDTTQV 
Sbjct: 301 VQSRMHRRSIRPMRM-ATRGVDVR-SSRRGTPDQDQDAALALRLQREEFLEAFRDTTQVH 360

Query: 361 TRSSLSLARANLRAMASRAAINLRINGRRNL 384
           TRSSLSL RAN+RA+ASRAAINLRI+G +NL
Sbjct: 361 TRSSLSLTRANIRAIASRAAINLRISGHQNL 382

BLAST of Sgr016616 vs. ExPASy TrEMBL
Match: A0A6J1F9B4 (E3 ubiquitin-protein ligase ORTHRUS 1-like OS=Cucurbita moschata OX=3662 GN=LOC111441989 PE=4 SV=1)

HSP 1 Score: 509.6 bits (1311), Expect = 1.1e-140
Identity = 290/389 (74.55%), Postives = 320/389 (82.26%), Query Frame = 0

Query: 1   MVPEGLTASASNPEPHTIESKPLEVGNGAESTFSPRFKSAAALAGWDEEALLIASLVVDD 60
           MV EG  ASAS PE   IES+PLE GN A+S FSPRFKSAAA+AGWDEEALLIASLVVDD
Sbjct: 1   MVQEGSRASASEPERRAIESRPLESGNDADSFFSPRFKSAAAMAGWDEEALLIASLVVDD 60

Query: 61  TPERGFRHKKRSVLQCKSPPSGSRRKRRTSTSLISSPVAVLNLDEEETTVKDDPPKQDPE 120
           TPER F+ KKRSVL CKSP SGSRRKRRTSTS+IS PVAVL+LD EE TV+DD  KQDPE
Sbjct: 61  TPEREFQQKKRSVLHCKSPTSGSRRKRRTSTSIISIPVAVLDLDAEEPTVRDDATKQDPE 120

Query: 121 ISVPETKKSDKLGEQNAG-ASFSCSALPCMEKLREELSCAICLEICFEPSTTPCGHSFCK 180
            +  E KKS+ L EQ AG +S SCS LPC++KLREELSCAICLEICFEPSTTPCGHSFCK
Sbjct: 121 TAEAEPKKSNSLIEQKAGDSSLSCSTLPCIDKLREELSCAICLEICFEPSTTPCGHSFCK 180

Query: 181 KCLRSAADKCGKRCPKCRQLISNGRSCTVNTVLWNTIQLLFPQEVDARK-EAKGCSRSQE 240
           KCLRSAAD+CGKRCPKCRQLISNGRSCTVNTVLWNTIQLLFPQEV+ARK  AK CS  ++
Sbjct: 181 KCLRSAADRCGKRCPKCRQLISNGRSCTVNTVLWNTIQLLFPQEVEARKVVAKECSSHEK 240

Query: 241 KIHNPEKAFY-------TRDTGTSSRHG-FTRRGGETTIR-DEDGEQDSGMIQRAVSSNA 300
           K+ +PE AFY       T+ T TSSR G  +RR GE T+R DEDGE+D+ ++Q+A S NA
Sbjct: 241 KVQDPENAFYSSLQNHNTQATDTSSRRGSSSRRRGEITVRDDEDGERDNRVMQQAASLNA 300

Query: 301 ESRVQSRMRRRSIRPVRMTTTRDVDVRSSSRRGTPDQDQDAALALRLQREEFMEAFRDTT 360
           ESRV SRM R+SIR +RM TTRD DV  SSRRGTPDQDQDAALALRLQREEFMEAFRDT 
Sbjct: 301 ESRVPSRMHRKSIRSMRM-TTRDADV--SSRRGTPDQDQDAALALRLQREEFMEAFRDTP 360

Query: 361 QVQTRSSLSLARANLRAMASRAAINLRIN 379
               RSSLSLARANLRAMASRAA++   N
Sbjct: 361 VQTRRSSLSLARANLRAMASRAAVSRHQN 386

BLAST of Sgr016616 vs. ExPASy TrEMBL
Match: A0A6J1DIW8 (E3 ubiquitin-protein ligase ORTHRUS 1-like OS=Momordica charantia OX=3673 GN=LOC111021497 PE=4 SV=1)

HSP 1 Score: 508.8 bits (1309), Expect = 1.9e-140
Identity = 289/392 (73.72%), Postives = 315/392 (80.36%), Query Frame = 0

Query: 1   MVPEGLTASASNPEPHTIESKPLEVGNGAESTFSPRFKSAAALAGWDEEALLIASLVVDD 60
           M+PEG        E   IESKPLE+GN ++  FSPRFKSAAA+AGWDEEALLIASLVVDD
Sbjct: 1   MLPEG-------SERQPIESKPLEIGNESQIFFSPRFKSAAAMAGWDEEALLIASLVVDD 60

Query: 61  TPERGFRHKKRSVLQCKSPPSGSRRKRRTSTSLISSPVAVLNLDEEETTVKDDPPKQDPE 120
           TPER  RH KR VLQ KSPPSGSRRKRRTSTSLIS PVAVL+LD+EE+ VKDDPPK+ PE
Sbjct: 61  TPERELRHNKRCVLQSKSPPSGSRRKRRTSTSLISIPVAVLDLDDEESAVKDDPPKERPE 120

Query: 121 ISVPETKKSDKLGEQNAGASFSCSALPCMEKLREELSCAICLEICFEPSTTPCGHSFCKK 180
            +V E KK+         +S S SALPCM+KLREELSCAICLEICFEPSTTPCGHSFCKK
Sbjct: 121 NAVGEAKKNG-----GGASSSSPSALPCMDKLREELSCAICLEICFEPSTTPCGHSFCKK 180

Query: 181 CLRSAADKCGKRCPKCRQLISNGRSCTVNTVLWNTIQLLFPQEVDARKEAKGCSRSQEKI 240
           CLRSAADKCGK+CPKCRQLISNGRSCTVNTVLWNTIQLLFP+EV+ARK+AK CSRSQEK+
Sbjct: 181 CLRSAADKCGKKCPKCRQLISNGRSCTVNTVLWNTIQLLFPKEVEARKQAKECSRSQEKV 240

Query: 241 HNPEKAFY---------TRDTGTSSRHGFTRRGGETTIRDEDGEQDSGMIQRAVSSNAES 300
            NPEKAFY         T  TGTSSRHG +RR GE  +        S M QRA SSNAES
Sbjct: 241 QNPEKAFYSSFQNHNTRTTGTGTSSRHGSSRRRGEELV--------SRMTQRAASSNAES 300

Query: 301 RVQSRMRRRSIRPVRMTTTRDVDVRSSSRRGTPDQDQDAALALRLQREEFMEAFRDTTQV 360
           RVQSR++RRSIRPVRM  TRD DV SS  R  PDQDQDAALALRLQREEFMEAFRD +QV
Sbjct: 301 RVQSRVQRRSIRPVRM-PTRDADVSSSLLRAAPDQDQDAALALRLQREEFMEAFRD-SQV 360

Query: 361 QTRSSLSLARANLRAMASRAAINLRINGRRNL 384
           QT SSLSLARANLRAMASRAA+N R+N R +L
Sbjct: 361 QTTSSLSLARANLRAMASRAAVNSRVNDRLDL 370

BLAST of Sgr016616 vs. ExPASy TrEMBL
Match: A0A5A7URU5 (LON peptidase N-terminal domain and RING finger protein 3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold108G00490 PE=4 SV=1)

HSP 1 Score: 499.2 bits (1284), Expect = 1.5e-137
Identity = 287/392 (73.21%), Postives = 313/392 (79.85%), Query Frame = 0

Query: 4   EGLTASASNPEPHTIESKPLEVGNGAESTFSPRFKSAAALAGWDEEALLIASLVVDDTPE 63
           EG TA  S       E KPL + N A+S  SPRFKSAAA+AGWDEEALLIASLVVDDTPE
Sbjct: 6   EGSTAGTS-------ELKPLGIANDADSFLSPRFKSAAAMAGWDEEALLIASLVVDDTPE 65

Query: 64  RGFRHKKRSVLQCKSP--PSGSRRKRRTSTSLISSPVAVLNLDEEETTVKDDPPKQDPEI 123
           R F+ KKRS LQ KSP   S SRRKRRT TS+IS PV VL+L+EEE  VKDD PKQ+P+ 
Sbjct: 66  REFQQKKRSDLQRKSPASASASRRKRRTLTSIISIPVTVLDLEEEEPAVKDDGPKQEPKY 125

Query: 124 SVPETKKSDKLGEQNAGA-SFSCSALPCMEKLREELSCAICLEICFEPSTTPCGHSFCKK 183
           +  E KK D + EQ A A S S S LPCM+KLREELSCAICLEICFEPSTTPCGHSFCKK
Sbjct: 126 AEAEAKKCDSMVEQKADASSLSSSTLPCMDKLREELSCAICLEICFEPSTTPCGHSFCKK 185

Query: 184 CLRSAADKCGKRCPKCRQLISNGRSCTVNTVLWNTIQLLFPQEVDARKEAKGCSRSQEKI 243
           CLRSAADKCGKRCPKCRQLISNGRSCTVNTVLWNTIQLLFP+EV+ARKE K C+  ++K 
Sbjct: 186 CLRSAADKCGKRCPKCRQLISNGRSCTVNTVLWNTIQLLFPKEVEARKEVKECNSHEKKN 245

Query: 244 HNPEKAFY-------TRDTGTSSRH--GFTRRGGETTIRDEDGEQDSGMIQRAVSSNAES 303
            +PEKAFY       TR TGTSSRH    +RR  E T+ +EDGE DS M QRA SSNAES
Sbjct: 246 QDPEKAFYSSLQNYNTRPTGTSSRHASNSSRRRAEITVGEEDGEWDSRMTQRAASSNAES 305

Query: 304 RVQSRMRRRSIRPVRMTTTRDVDVRSSSRRGTPDQDQDAALALRLQREEFMEAFRDTTQV 363
           RVQSRM RRSIRP+RM  TR  DVR SSRRGTPDQDQDAALALRLQREEF+EAFRDTTQV
Sbjct: 306 RVQSRMHRRSIRPMRM-ATRGADVR-SSRRGTPDQDQDAALALRLQREEFLEAFRDTTQV 365

Query: 364 QTRSSLSLARANLRAMASRAAINLRINGRRNL 384
            TRSSLSL RAN+RA+ASRAAINLRI G +NL
Sbjct: 366 HTRSSLSLTRANIRAIASRAAINLRIGGHQNL 388

BLAST of Sgr016616 vs. ExPASy TrEMBL
Match: A0A6J1J0X7 (E3 ubiquitin-protein ligase TRIM39-like OS=Cucurbita maxima OX=3661 GN=LOC111482456 PE=4 SV=1)

HSP 1 Score: 497.7 bits (1280), Expect = 4.4e-137
Identity = 289/392 (73.72%), Postives = 313/392 (79.85%), Query Frame = 0

Query: 1   MVPEGLTASASNPEPHTIESKPLEVGNGAESTFSPRFKSAAALAGWDEEALLIASLVVDD 60
           MV EG TASAS PE   IES+PLE GN A+S FSPRFKSAAA+AGWDEEALLIASLVVDD
Sbjct: 1   MVQEGSTASASEPEQRVIESRPLECGNDADSFFSPRFKSAAAMAGWDEEALLIASLVVDD 60

Query: 61  TPERGFRHKKRSVLQCKSPPSGSRRKRRTSTSLISSPVAVLNLDEEETTVKDDPPKQDPE 120
           TPER F+ KKRSVL CKSP SGSRRKRR STS IS PVAVL+LD EE TVKDD  KQDPE
Sbjct: 61  TPEREFQQKKRSVLHCKSPTSGSRRKRRISTSKISIPVAVLDLDAEEPTVKDDATKQDPE 120

Query: 121 ISVPETKKSDKLGEQNAGA-SFSCSALPCMEKLREELSCAICLEICFEPSTTPCGHSFCK 180
            +  E KKSD L EQ AGA S SCS LP ++KLREELSCAICLEICFEPSTTPCGHSFCK
Sbjct: 121 TAEAEPKKSDSLIEQKAGASSLSCSTLPFIDKLREELSCAICLEICFEPSTTPCGHSFCK 180

Query: 181 KCLRSAADKCGKRCPKCRQLISNGRSCTVNTVLWNTIQLLFPQEVDARK--EAKGCSRSQ 240
           KCLRSAAD+CGKRCPKCRQLISNGRSCTVNTVLWNTIQLLFPQEV+ARK   AK CS  +
Sbjct: 181 KCLRSAADRCGKRCPKCRQLISNGRSCTVNTVLWNTIQLLFPQEVEARKVVVAKECSSHE 240

Query: 241 EKIHNPEKAFYT---------RDTGTSSRHG-FTRRGGETTIR-DEDGEQDSGMIQRAVS 300
           +K+ +PEKAFY+         + T T SR G  +RR GE T+R DED E D+ ++Q+A S
Sbjct: 241 KKVQDPEKAFYSSLQNHNTNRQATDTLSRRGSSSRRRGEITVRDDEDDEWDNRVMQQAAS 300

Query: 301 SNAESRVQSRMRRRSIRPVRMTTTRDVDVRSSSRRGTPDQDQDAALALRLQREEFMEAFR 360
           SNAESRV SRM RRSIR +RM  TRD D   SSRRGTPDQDQDAALALRLQREEFMEA R
Sbjct: 301 SNAESRVPSRMHRRSIRSLRM-ATRDAD--ESSRRGTPDQDQDAALALRLQREEFMEALR 360

Query: 361 DTTQVQTRSSLSLARANLRAMASRAAINLRIN 379
           DT     RSSLSLARANLRAMASRAA +   N
Sbjct: 361 DTPVQTRRSSLSLARANLRAMASRAAASRHRN 389

BLAST of Sgr016616 vs. TAIR 10
Match: AT3G27330.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 261.2 bits (666), Expect = 1.3e-69
Identity = 182/359 (50.70%), Postives = 225/359 (62.67%), Query Frame = 0

Query: 26  GNGAESTFSPRFKSAAALAGWDEEALLIASLVVDDTPER-GFRHKKRSVLQCKS-PPSG- 85
           G  AE   SPRFKSAAA+AGWDEE L+IAS VVDDTPER   + ++RS L  KS PPSG 
Sbjct: 590 GENAEIFISPRFKSAAAMAGWDEEDLIIASFVVDDTPERSSSKRRRRSNLLFKSTPPSGS 649

Query: 86  SRRKRRTSTSLISSPVAVLNLD-----EEETTVKDDPPKQDPEISVPETKKSDKLGEQNA 145
           SRR +R   SL+  P+ V++LD     EEE + +    K++ E    E KK DK    + 
Sbjct: 650 SRRNQRVKQSLV--PLPVIDLDKVIRHEEEKSAEKKKKKREMETETKEEKKIDK----DE 709

Query: 146 GASFSCSALPCMEKLREELSCAICLEICFEPSTTPCGHSFCKKCLRSAADKCGKRCPKCR 205
            +      LPC++KLR+ELSCAICLEICFEPSTT CGHSFCKKCLRSAADKCG++CPKCR
Sbjct: 710 KSLSDHVVLPCIDKLRDELSCAICLEICFEPSTTTCGHSFCKKCLRSAADKCGRKCPKCR 769

Query: 206 QLISNGRSCTVNTVLWNTIQLLFPQEVDARKEAKGCSRSQE--KIHNPEKAFYTRDTGTS 265
           QLI NGR CTVNTVLWNTIQLLFP+EV+A++ A    + +E     NP +   +R+    
Sbjct: 770 QLIGNGRYCTVNTVLWNTIQLLFPKEVEAQRAASANLKGKETPSPRNPNQRLRSRNR--- 829

Query: 266 SRHGFTRRGGETTIRDEDGEQDSGMIQRAVSSNAESRVQSRMRRRSIRPVRMTTTRDVDV 325
                     ET +  E  +++   I R + S  E R +   RRR               
Sbjct: 830 ----------ETALFQERLQRED--ISRLLVS--EERSERSERRR--------------- 889

Query: 326 RSSSRRGTPDQDQDAALALRLQREEFMEAFRDTTQVQTRSS---LSLARANLRAMASRA 372
           RS+S R   DQ++DAALA+RLQR+EF  AF  TT   + SS   LS ARANL+AMASRA
Sbjct: 890 RSTSMR--LDQNRDAALAMRLQRQEFASAFGATTAETSSSSVSTLSRARANLQAMASRA 908

BLAST of Sgr016616 vs. TAIR 10
Match: AT1G18660.4 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 76.3 bits (186), Expect = 6.2e-14
Identity = 34/81 (41.98%), Postives = 52/81 (64.20%), Query Frame = 0

Query: 149 MEKLREELSCAICLEICFEPSTTPCGHSFCKKCLRSAADKCGKRCPKCRQLI-SNGRSCT 208
           M +  ++  C +CL++ +EP+TTPCGH+FC+ CL  + D+ G +CP CR +I    R+C 
Sbjct: 187 MAERSDDFDCTVCLKLLYEPATTPCGHTFCRSCLFQSMDR-GNKCPLCRTVIFMTPRTCA 246

Query: 209 VNTVLWNTIQLLFPQEVDARK 229
           V+  L N I+  FP+E   RK
Sbjct: 247 VSVTLNNIIEKNFPEEYAERK 266

BLAST of Sgr016616 vs. TAIR 10
Match: AT1G18660.2 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 76.3 bits (186), Expect = 6.2e-14
Identity = 34/81 (41.98%), Postives = 52/81 (64.20%), Query Frame = 0

Query: 149 MEKLREELSCAICLEICFEPSTTPCGHSFCKKCLRSAADKCGKRCPKCRQLI-SNGRSCT 208
           M +  ++  C +CL++ +EP+TTPCGH+FC+ CL  + D+ G +CP CR +I    R+C 
Sbjct: 187 MAERSDDFDCTVCLKLLYEPATTPCGHTFCRSCLFQSMDR-GNKCPLCRTVIFMTPRTCA 246

Query: 209 VNTVLWNTIQLLFPQEVDARK 229
           V+  L N I+  FP+E   RK
Sbjct: 247 VSVTLNNIIEKNFPEEYAERK 266

BLAST of Sgr016616 vs. TAIR 10
Match: AT1G18660.3 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 76.3 bits (186), Expect = 6.2e-14
Identity = 34/81 (41.98%), Postives = 52/81 (64.20%), Query Frame = 0

Query: 149 MEKLREELSCAICLEICFEPSTTPCGHSFCKKCLRSAADKCGKRCPKCRQLI-SNGRSCT 208
           M +  ++  C +CL++ +EP+TTPCGH+FC+ CL  + D+ G +CP CR +I    R+C 
Sbjct: 187 MAERSDDFDCTVCLKLLYEPATTPCGHTFCRSCLFQSMDR-GNKCPLCRTVIFMTPRTCA 246

Query: 209 VNTVLWNTIQLLFPQEVDARK 229
           V+  L N I+  FP+E   RK
Sbjct: 247 VSVTLNNIIEKNFPEEYAERK 266

BLAST of Sgr016616 vs. TAIR 10
Match: AT1G18660.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 76.3 bits (186), Expect = 6.2e-14
Identity = 34/81 (41.98%), Postives = 52/81 (64.20%), Query Frame = 0

Query: 149 MEKLREELSCAICLEICFEPSTTPCGHSFCKKCLRSAADKCGKRCPKCRQLI-SNGRSCT 208
           M +  ++  C +CL++ +EP+TTPCGH+FC+ CL  + D+ G +CP CR +I    R+C 
Sbjct: 187 MAERSDDFDCTVCLKLLYEPATTPCGHTFCRSCLFQSMDR-GNKCPLCRTVIFMTPRTCA 246

Query: 209 VNTVLWNTIQLLFPQEVDARK 229
           V+  L N I+  FP+E   RK
Sbjct: 247 VSVTLNNIIEKNFPEEYAERK 266