Sgr016575 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr016575 vs. NCBI nr
Match: XP_022156536.1 (probable inactive shikimate kinase like 1, chloroplastic [Momordica charantia])

HSP 1 Score: 380.2 bits (975), Expect = 2.0e-101
Identity = 237/362 (65.47%), Postives = 258/362 (71.27%), Query Frame = 0

Query: 3   MKLKTTVSSARPDLQFYVRLLNVQSMALNLNCSLPLPSSLRRKHNLPLHVRRRFLDSSAN 62
           M +K TVSS+RPDLQF +R   + +MALNL+CSLPLPSS+ RKH LPLHV  RFL SSA 
Sbjct: 1   MAMKNTVSSSRPDLQFSIRSPKIHAMALNLSCSLPLPSSVHRKHILPLHVSGRFLHSSAI 60

Query: 63  RRRRSLPTTCAVSDGTAFSEFKFGIHPLPQDDLSWALLDVEAKVTVDDLSLEVKKKAMDV 122
           +R RSLP+TC  SDG A                    L+VEAKVTVDDLSLEV+KKAMDV
Sbjct: 61  QRWRSLPSTCLASDGAA--------------------LNVEAKVTVDDLSLEVQKKAMDV 120

Query: 123 APELKELPSFSWVSNNLQFWRNFGSYRDAFSFWACGNVFLPNLL--ILNFYMSHANAYSD 182
           APELK    F    N+ Q                     L  LL  +L +Y       SD
Sbjct: 121 APELKGTSIFLVGINSSQ------------------KTKLGRLLADVLRYYYFD----SD 180

Query: 183 SLVVEASGGAAAAKLYKESDENGFRASETEVLNQLSSMGRLVICAGNGAVQSSTNLYERS 242
           SLVVEASGGAAAAKLYKESDENGFRASETEVL QLSSMGRLV+CAGNGAVQSSTNL   +
Sbjct: 181 SLVVEASGGAAAAKLYKESDENGFRASETEVLKQLSSMGRLVVCAGNGAVQSSTNL---A 240

Query: 243 MLNFPGSVHTGTFEAWHIIMDDLPLQMIAEEFAEDGSQLPVLDISTSGSYSEVLGQITSL 302
           +L      H  T      +  DLPLQMIAEEFAED SQLP  DIST GSYSEVLGQITSL
Sbjct: 241 LLR-----HGIT------LWIDLPLQMIAEEFAEDHSQLPAFDISTFGSYSEVLGQITSL 300

Query: 303 YQEIKDGYATADASISLQKLASELGYDDLNAVTTEDMALEALKELEKLTRVKKMIEAAAK 362
           YQE+KDGYATADASISLQKLAS+ GYDDLNAVTTEDMALEALKE+EKLTRVKKMIEAAAK
Sbjct: 301 YQEMKDGYATADASISLQKLASKSGYDDLNAVTTEDMALEALKEIEKLTRVKKMIEAAAK 306

BLAST of Sgr016575 vs. NCBI nr
Match: KAA0060353.1 (putative inactive shikimate kinase like 1 [Cucumis melo var. makuwa] >TYK22102.1 putative inactive shikimate kinase like 1 [Cucumis melo var. makuwa])

HSP 1 Score: 356.3 bits (913), Expect = 3.1e-94
Identity = 226/362 (62.43%), Postives = 254/362 (70.17%), Query Frame = 0

Query: 1   MAMKLKTTVSSARPDLQFYVRLLNVQSMALNLNCSLPLPSSLRRKHNLPLHVRRRFLDSS 60
           MAM LKTTVS    DLQF + L   ++MALN + SL LPSSLRR H+LPLH+   F+ SS
Sbjct: 88  MAMNLKTTVSPL-TDLQFNIHLPKFEAMALNSSFSLSLPSSLRRNHSLPLHICPPFIQSS 147

Query: 61  ANRRRRSLPTTCAVSDGTAFSEFKFGIHPLPQDDLSWALLDVEAKVTVDDLSLEVKKKAM 120
           A RRRRSLP++C VSDGT+                    L+VEAKVTVDDLSLEVKKKAM
Sbjct: 148 AIRRRRSLPSSCLVSDGTS--------------------LNVEAKVTVDDLSLEVKKKAM 207

Query: 121 DVAPELKELPSFSWVSNNLQFWRNFGSYRDAFSFWACGNVFLPNLLILNFYMSHANAYSD 180
           DV PELK    F  V  N     N G               L ++L   ++       SD
Sbjct: 208 DVGPELKGTSIF-LVGINSSIKTNLGK-------------LLADVLRYYYF------DSD 267

Query: 181 SLVVEASGGAAAAKLYKESDENGFRASETEVLNQLSSMGRLVICAGNGAVQSSTNLYERS 240
           SLVVEASGG AAAKLYK+SDE GF+ASETEVL QLSSMGRLV+CAG+GAVQSSTNL   +
Sbjct: 268 SLVVEASGGEAAAKLYKQSDEKGFQASETEVLKQLSSMGRLVVCAGSGAVQSSTNL---A 327

Query: 241 MLNFPGSVHTGTFEAWHIIMDDLPLQMIAEEFAEDGSQLPVLDISTSGSYSEVLGQITSL 300
           +L      H  T      +  DLPLQMIAEEFAED SQ PV DISTSGSYSEVLGQITS+
Sbjct: 328 LLR-----HGIT------LWIDLPLQMIAEEFAEDRSQHPVFDISTSGSYSEVLGQITSI 387

Query: 301 YQEIKDGYATADASISLQKLASELGYDDLNAVTTEDMALEALKELEKLTRVKKMIEAAAK 360
           YQ++KDGYATADA ISLQKLAS+LGYDD NAVTTEDMA+EALKE+EKLTRVKKMIEAAAK
Sbjct: 388 YQKVKDGYATADALISLQKLASKLGYDDFNAVTTEDMAMEALKEIEKLTRVKKMIEAAAK 394

Query: 361 PF 363
           PF
Sbjct: 448 PF 394

BLAST of Sgr016575 vs. NCBI nr
Match: XP_008450164.1 (PREDICTED: probable inactive shikimate kinase like 1, chloroplastic [Cucumis melo])

HSP 1 Score: 356.3 bits (913), Expect = 3.1e-94
Identity = 226/362 (62.43%), Postives = 254/362 (70.17%), Query Frame = 0

Query: 1   MAMKLKTTVSSARPDLQFYVRLLNVQSMALNLNCSLPLPSSLRRKHNLPLHVRRRFLDSS 60
           MAM LKTTVS    DLQF + L   ++MALN + SL LPSSLRR H+LPLH+   F+ SS
Sbjct: 1   MAMNLKTTVSPL-TDLQFNIHLPKFEAMALNSSFSLSLPSSLRRNHSLPLHICPPFIQSS 60

Query: 61  ANRRRRSLPTTCAVSDGTAFSEFKFGIHPLPQDDLSWALLDVEAKVTVDDLSLEVKKKAM 120
           A RRRRSLP++C VSDGT+                    L+VEAKVTVDDLSLEVKKKAM
Sbjct: 61  AIRRRRSLPSSCLVSDGTS--------------------LNVEAKVTVDDLSLEVKKKAM 120

Query: 121 DVAPELKELPSFSWVSNNLQFWRNFGSYRDAFSFWACGNVFLPNLLILNFYMSHANAYSD 180
           DV PELK    F  V  N     N G               L ++L   ++       SD
Sbjct: 121 DVGPELKGTSIF-LVGINSSIKTNLGK-------------LLADVLRYYYF------DSD 180

Query: 181 SLVVEASGGAAAAKLYKESDENGFRASETEVLNQLSSMGRLVICAGNGAVQSSTNLYERS 240
           SLVVEASGG AAAKLYK+SDE GF+ASETEVL QLSSMGRLV+CAG+GAVQSSTNL   +
Sbjct: 181 SLVVEASGGEAAAKLYKQSDEKGFQASETEVLKQLSSMGRLVVCAGSGAVQSSTNL---A 240

Query: 241 MLNFPGSVHTGTFEAWHIIMDDLPLQMIAEEFAEDGSQLPVLDISTSGSYSEVLGQITSL 300
           +L      H  T      +  DLPLQMIAEEFAED SQ PV DISTSGSYSEVLGQITS+
Sbjct: 241 LLR-----HGIT------LWIDLPLQMIAEEFAEDRSQHPVFDISTSGSYSEVLGQITSI 300

Query: 301 YQEIKDGYATADASISLQKLASELGYDDLNAVTTEDMALEALKELEKLTRVKKMIEAAAK 360
           YQ++KDGYATADA ISLQKLAS+LGYDD NAVTTEDMA+EALKE+EKLTRVKKMIEAAAK
Sbjct: 301 YQKVKDGYATADALISLQKLASKLGYDDFNAVTTEDMAMEALKEIEKLTRVKKMIEAAAK 307

Query: 361 PF 363
           PF
Sbjct: 361 PF 307

BLAST of Sgr016575 vs. NCBI nr
Match: XP_038877384.1 (probable inactive shikimate kinase like 1, chloroplastic [Benincasa hispida])

HSP 1 Score: 353.2 bits (905), Expect = 2.6e-93
Identity = 226/362 (62.43%), Postives = 252/362 (69.61%), Query Frame = 0

Query: 1   MAMKLKTTVSSARPDLQFYVRLLNVQSMALNLNCSLPLPSSLRRKHNLPLHVRRRFLDSS 60
           + M +KTTVSS   DLQF + L   ++MALN + SL LP SL R H+ PLH+   F+ SS
Sbjct: 24  LLMAMKTTVSSL-TDLQFNIYLPKFEAMALNSSFSLSLPPSLCRNHSFPLHICPPFMQSS 83

Query: 61  ANRRRRSLPTTCAVSDGTAFSEFKFGIHPLPQDDLSWALLDVEAKVTVDDLSLEVKKKAM 120
           A RRRRSLP+TC VSDGTAF                    +VEAKVTVDDL LEVKKKAM
Sbjct: 84  AIRRRRSLPSTCLVSDGTAF--------------------NVEAKVTVDDLPLEVKKKAM 143

Query: 121 DVAPELKELPSFSWVSNNLQFWRNFGSYRDAFSFWACGNVFLPNLLILNFYMSHANAYSD 180
           DVAPELK    F  V  N       G              FL +LL   ++       SD
Sbjct: 144 DVAPELKGTSIF-LVGINSSIKTKLGK-------------FLADLLRYYYF------DSD 203

Query: 181 SLVVEASGGAAAAKLYKESDENGFRASETEVLNQLSSMGRLVICAGNGAVQSSTNLYERS 240
           SLVVEASGG AAAKLYK++DENGF+ASE+EVL QLSSMGRLV+CAGNGAVQSSTNL   +
Sbjct: 204 SLVVEASGGEAAAKLYKQNDENGFQASESEVLKQLSSMGRLVVCAGNGAVQSSTNL---A 263

Query: 241 MLNFPGSVHTGTFEAWHIIMDDLPLQMIAEEFAEDGSQLPVLDISTSGSYSEVLGQITSL 300
           +L      H  T      +  DLPLQMIAEEFAED SQ PV DISTSGSYSEVL QITSL
Sbjct: 264 LLR-----HGIT------LWIDLPLQMIAEEFAEDRSQHPVFDISTSGSYSEVLAQITSL 323

Query: 301 YQEIKDGYATADASISLQKLASELGYDDLNAVTTEDMALEALKELEKLTRVKKMIEAAAK 360
           YQ+IKDGYATADASISLQKLAS+LGYDD NAVTTED+ALEALKE+EKLTRVKKMIEAAAK
Sbjct: 324 YQKIKDGYATADASISLQKLASKLGYDDFNAVTTEDLALEALKEIEKLTRVKKMIEAAAK 330

Query: 361 PF 363
           PF
Sbjct: 384 PF 330

BLAST of Sgr016575 vs. NCBI nr
Match: XP_022989694.1 (probable inactive shikimate kinase like 1, chloroplastic isoform X1 [Cucurbita maxima])

HSP 1 Score: 351.7 bits (901), Expect = 7.7e-93
Identity = 223/360 (61.94%), Postives = 246/360 (68.33%), Query Frame = 0

Query: 3   MKLKTTVSSARPDLQFYVRLLNVQSMALNLNCSLPLPSSLRRKHNLPLHVRRRFLDSSAN 62
           M +KTTVS    D QF + L  +Q M+LN + +LPLP S+R   NLPLH RRRFL SS  
Sbjct: 1   MAMKTTVSPL-TDFQFNIHLPKIQPMSLNSSYALPLPFSVRCNQNLPLHFRRRFLHSSVI 60

Query: 63  RRRRSLPTTCAVSDGTAFSEFKFGIHPLPQDDLSWALLDVEAKVTVDDLSLEVKKKAMDV 122
           +RRRSLP+TC VSDGTA+                    +VEAK T DDLSLEVKKKAMDV
Sbjct: 61  QRRRSLPSTCFVSDGTAY--------------------NVEAKATTDDLSLEVKKKAMDV 120

Query: 123 APELKELPSFSWVSNNLQFWRNFGSYRDAFSFWACGNVFLPNLLILNFYMSHANAYSDSL 182
           APELK    F  V  N       G               L ++  L +Y       SD L
Sbjct: 121 APELKGTSIF-LVGINSSLKTKLGK-------------LLADM--LRYYYFDRYFLSDGL 180

Query: 183 VVEASGGAAAAKLYKESDENGFRASETEVLNQLSSMGRLVICAGNGAVQSSTNLYERSML 242
           VVEASGG AAAKLYKESDENGFRASETEVL QLSSMGRLV+CAGNGAV+SSTNL   ++L
Sbjct: 181 VVEASGGVAAAKLYKESDENGFRASETEVLKQLSSMGRLVVCAGNGAVRSSTNL---ALL 240

Query: 243 NFPGSVHTGTFEAWHIIMDDLPLQMIAEEFAEDGSQLPVLDISTSGSYSEVLGQITSLYQ 302
                 H  T      +  D+PLQ+IAEEFAED  QLPV DISTSGSYSEVLGQITSLYQ
Sbjct: 241 R-----HGIT------LWIDIPLQIIAEEFAEDRGQLPVFDISTSGSYSEVLGQITSLYQ 300

Query: 303 EIKDGYATADASISLQKLASELGYDDLNAVTTEDMALEALKELEKLTRVKKMIEAAAKPF 362
           EIK GYATADASISLQKLAS LGYDD NAVTTEDMALEALKE+EKL RVKKM+EAAAKPF
Sbjct: 301 EIKVGYATADASISLQKLASTLGYDDFNAVTTEDMALEALKEIEKLIRVKKMMEAAAKPF 309

BLAST of Sgr016575 vs. ExPASy Swiss-Prot
Match: Q9LW20 (Probable inactive shikimate kinase like 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=SKL1 PE=2 SV=1)

HSP 1 Score: 146.4 bits (368), Expect = 6.4e-34
Identity = 105/248 (42.34%), Postives = 143/248 (57.66%), Query Frame = 0

Query: 115 VKKKAMDVAPELKELPSFSWVSNNLQFWRNFGSYRDAFSFWACGNVFLPNLLILNFYMSH 174
           VK+KAMD+APELK    F  V  N     N G                    +L   + +
Sbjct: 76  VKRKAMDIAPELKGASIF-LVGINNSIKTNTGK-------------------LLAEALRY 135

Query: 175 ANAYSDSLVVEASGGAAAAKLYKESDENGFRASETEVLNQLSSMGRLVICAGNGAVQSST 234
               SD+L+ EA+GG  +A+  KE+DE  F+ SETEVL QLSSMGRLV+CAG+GAVQS  
Sbjct: 136 YYFDSDNLITEAAGGNVSAQALKEADEKAFQESETEVLKQLSSMGRLVVCAGDGAVQSLR 195

Query: 235 NLYERSMLNFPGSVHTGTFEAWHIIMDDLPLQMIAEEFAEDGSQLPVLDISTSGSYSEVL 294
           NL   ++L    S           I  D+PL + A+   +D       D   S    E+ 
Sbjct: 196 NL---ALLRHGIS-----------IWIDVPLDITAK--GDD-------DSFHSEPSPELF 255

Query: 295 GQITSLYQEIKDGYATADASISLQKLASELGYDDLNAVTTEDMALEALKELEKLTRVKKM 354
             + + Y++ + GY TAD SISL+K+A++L ++DL AVT+ED+ALE LKE+EKLTRVKKM
Sbjct: 256 DTLKASYEKSRKGYETADVSISLEKIATKLEFEDLEAVTSEDIALEILKEIEKLTRVKKM 280

Query: 355 IEAAAKPF 363
           +E A++PF
Sbjct: 316 MEEASRPF 280

BLAST of Sgr016575 vs. ExPASy Swiss-Prot
Match: Q5NTH3 (Shikimate kinase 2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=SK2 PE=1 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 8.4e-18
Identity = 60/170 (35.29%), Postives = 97/170 (57.06%), Query Frame = 0

Query: 179 SDSLVVEASGGAAAAKLYKESDENGFRASETEVLNQLSSMGRLVICAGNGAVQSSTN-LY 238
           SD LV +A G  + A+++KE  E  FR +E+ VL  LSSM RLV+  G GAV    N  Y
Sbjct: 125 SDKLVEQAVGMPSVAQIFKEHSEAFFRDNESSVLRDLSSMRRLVVATGGGAVIRPVNWKY 184

Query: 239 ERSMLNFPGSVHTGTFEAWHIIMDDLPLQMIAEEFAEDG-SQLPVLDISTSGSYSEVLGQ 298
            +  L+               +  D+PL  +A   A+ G +  P+LD  +S  Y+    +
Sbjct: 185 MKKGLS---------------VWLDVPLDALARRIAQVGTASRPLLDQPSSDPYTAAFSK 244

Query: 299 ITSLYQEIKDGYATADASISLQKLASELGYDDLNAVTTEDMALEALKELE 347
           ++ L ++  D YA ADA +SL+++A++ G+DD++ +T  D+A+EAL ++E
Sbjct: 245 LSMLAEQRGDAYANADARVSLEEIAAKQGHDDVSKLTPTDIAIEALLKIE 279

BLAST of Sgr016575 vs. ExPASy Swiss-Prot
Match: Q5NTH4 (Shikimate kinase 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=SK1 PE=1 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 1.8e-15
Identity = 56/170 (32.94%), Postives = 93/170 (54.71%), Query Frame = 0

Query: 179 SDSLVVEASGGAAAAKLYKESDENGFRASETEVLNQLSSMGRLVICAGNGAVQSSTN-LY 238
           SD LV +A G  + A+++K   E  FR +E+ VL  LSSM RLV+  G GAV    N  Y
Sbjct: 127 SDKLVEQAVGMPSVAQIFKVHSEAFFRDNESSVLRDLSSMKRLVVATGGGAVIRPVNWKY 186

Query: 239 ERSMLNFPGSVHTGTFEAWHIIMDDLPLQMIAEEFAEDG-SQLPVLDISTSGSYSEVLGQ 298
            +  L+               +  D+PL  +A   A+ G +  P+LD  +   Y+    +
Sbjct: 187 MKKGLS---------------VWLDVPLDALARRIAKVGTASRPLLDQPSGDPYTMAFSK 246

Query: 299 ITSLYQEIKDGYATADASISLQKLASELGYDDLNAVTTEDMALEALKELE 347
           ++ L ++  D YA AD  +SL+++AS+ G+DD++ +T  D+A+E+  ++E
Sbjct: 247 LSMLAEQRGDAYANADVRVSLEEIASKQGHDDVSKLTPTDIAIESFHKIE 281

BLAST of Sgr016575 vs. ExPASy Swiss-Prot
Match: Q7X7H9 (Shikimate kinase 3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=SK3 PE=1 SV=2)

HSP 1 Score: 82.8 bits (203), Expect = 8.7e-15
Identity = 57/178 (32.02%), Postives = 93/178 (52.25%), Query Frame = 0

Query: 179 SDSLVVEASGGAAAAKLYKESDENGFRASETEVLNQLSSMGRLVICAGNGAVQSSTNLYE 238
           SD LV +A G ++ A++++   E  FR +E+EVL  LSSM RLV+  G GAV    N   
Sbjct: 122 SDKLVEKAVGISSVAEIFQLHSEAFFRDNESEVLRDLSSMHRLVVATGGGAVIRPIN--- 181

Query: 239 RSMLNFPGSVHTGTFEAWHIIMDDLPLQMIAEEFAEDG-SQLPVLDISTSGSYSEVLGQI 298
                          +    I  D+PL  +A   A  G +  P+L   +   Y++   ++
Sbjct: 182 -----------WSYMKKGSTIWLDVPLDALARRIAAVGTASRPLLHQESGDPYAKAYAKL 241

Query: 299 TSLYQEIKDGYATADASISLQKLASELGYDDLNAVTTEDMALEALKELEKLTRVKKMI 356
           T+L+++  D YA ADA +SL+ +A + G+ ++  +T   +A+EAL ++E     K MI
Sbjct: 242 TALFEQRMDSYANADARVSLEHIAVKQGHSNVTTLTPSAIAIEALLKMESFLTEKAMI 285

BLAST of Sgr016575 vs. ExPASy Swiss-Prot
Match: Q00497 (Shikimate kinase, chloroplastic OS=Solanum lycopersicum OX=4081 GN=SK PE=1 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 1.5e-11
Identity = 53/177 (29.94%), Postives = 92/177 (51.98%), Query Frame = 0

Query: 180 DSLVVEASGGAAAAKLYKESDENGFRASETEVLNQLSSMGRLVICAGNGAVQSSTNLYER 239
           D L+ +A GG   A++++   E+ FR +ETEVL++LS M RLV+  G GAV    N    
Sbjct: 136 DRLIEQAVGGITVAEIFELRGESFFRDNETEVLHKLSLMHRLVVSTGGGAVVRPINWRH- 195

Query: 240 SMLNFPGSVHTGTFEAWHIIMDDLPLQMIAEEFAEDGSQ-LPVLDISTSGSYSEVLGQIT 299
                   +H G    W     D+PL+ +A+    +G++  P+L   +   Y   L ++T
Sbjct: 196 --------MHKG-ISVWL----DVPLEALAKRITTEGTKSRPLLHEESGDVYDTTLKRLT 255

Query: 300 SLYQEIKDGYATADASISLQKLASELGYDDLNAVTTEDMALEALKELEKLTRVKKMI 356
           +L +   + YA A A +SL+ +A +    D+  +T  ++ LE L ++E   + +K +
Sbjct: 256 TLMETRGENYANASARVSLENIALK-REKDVCHITPAEITLEVLIQIENFLKTQKSV 297

BLAST of Sgr016575 vs. ExPASy TrEMBL
Match: A0A6J1DV91 (probable inactive shikimate kinase like 1, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111023415 PE=3 SV=1)

HSP 1 Score: 380.2 bits (975), Expect = 9.8e-102
Identity = 237/362 (65.47%), Postives = 258/362 (71.27%), Query Frame = 0

Query: 3   MKLKTTVSSARPDLQFYVRLLNVQSMALNLNCSLPLPSSLRRKHNLPLHVRRRFLDSSAN 62
           M +K TVSS+RPDLQF +R   + +MALNL+CSLPLPSS+ RKH LPLHV  RFL SSA 
Sbjct: 1   MAMKNTVSSSRPDLQFSIRSPKIHAMALNLSCSLPLPSSVHRKHILPLHVSGRFLHSSAI 60

Query: 63  RRRRSLPTTCAVSDGTAFSEFKFGIHPLPQDDLSWALLDVEAKVTVDDLSLEVKKKAMDV 122
           +R RSLP+TC  SDG A                    L+VEAKVTVDDLSLEV+KKAMDV
Sbjct: 61  QRWRSLPSTCLASDGAA--------------------LNVEAKVTVDDLSLEVQKKAMDV 120

Query: 123 APELKELPSFSWVSNNLQFWRNFGSYRDAFSFWACGNVFLPNLL--ILNFYMSHANAYSD 182
           APELK    F    N+ Q                     L  LL  +L +Y       SD
Sbjct: 121 APELKGTSIFLVGINSSQ------------------KTKLGRLLADVLRYYYFD----SD 180

Query: 183 SLVVEASGGAAAAKLYKESDENGFRASETEVLNQLSSMGRLVICAGNGAVQSSTNLYERS 242
           SLVVEASGGAAAAKLYKESDENGFRASETEVL QLSSMGRLV+CAGNGAVQSSTNL   +
Sbjct: 181 SLVVEASGGAAAAKLYKESDENGFRASETEVLKQLSSMGRLVVCAGNGAVQSSTNL---A 240

Query: 243 MLNFPGSVHTGTFEAWHIIMDDLPLQMIAEEFAEDGSQLPVLDISTSGSYSEVLGQITSL 302
           +L      H  T      +  DLPLQMIAEEFAED SQLP  DIST GSYSEVLGQITSL
Sbjct: 241 LLR-----HGIT------LWIDLPLQMIAEEFAEDHSQLPAFDISTFGSYSEVLGQITSL 300

Query: 303 YQEIKDGYATADASISLQKLASELGYDDLNAVTTEDMALEALKELEKLTRVKKMIEAAAK 362
           YQE+KDGYATADASISLQKLAS+ GYDDLNAVTTEDMALEALKE+EKLTRVKKMIEAAAK
Sbjct: 301 YQEMKDGYATADASISLQKLASKSGYDDLNAVTTEDMALEALKEIEKLTRVKKMIEAAAK 306

BLAST of Sgr016575 vs. ExPASy TrEMBL
Match: A0A5D3DET0 (Putative inactive shikimate kinase like 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold318G00920 PE=3 SV=1)

HSP 1 Score: 356.3 bits (913), Expect = 1.5e-94
Identity = 226/362 (62.43%), Postives = 254/362 (70.17%), Query Frame = 0

Query: 1   MAMKLKTTVSSARPDLQFYVRLLNVQSMALNLNCSLPLPSSLRRKHNLPLHVRRRFLDSS 60
           MAM LKTTVS    DLQF + L   ++MALN + SL LPSSLRR H+LPLH+   F+ SS
Sbjct: 88  MAMNLKTTVSPL-TDLQFNIHLPKFEAMALNSSFSLSLPSSLRRNHSLPLHICPPFIQSS 147

Query: 61  ANRRRRSLPTTCAVSDGTAFSEFKFGIHPLPQDDLSWALLDVEAKVTVDDLSLEVKKKAM 120
           A RRRRSLP++C VSDGT+                    L+VEAKVTVDDLSLEVKKKAM
Sbjct: 148 AIRRRRSLPSSCLVSDGTS--------------------LNVEAKVTVDDLSLEVKKKAM 207

Query: 121 DVAPELKELPSFSWVSNNLQFWRNFGSYRDAFSFWACGNVFLPNLLILNFYMSHANAYSD 180
           DV PELK    F  V  N     N G               L ++L   ++       SD
Sbjct: 208 DVGPELKGTSIF-LVGINSSIKTNLGK-------------LLADVLRYYYF------DSD 267

Query: 181 SLVVEASGGAAAAKLYKESDENGFRASETEVLNQLSSMGRLVICAGNGAVQSSTNLYERS 240
           SLVVEASGG AAAKLYK+SDE GF+ASETEVL QLSSMGRLV+CAG+GAVQSSTNL   +
Sbjct: 268 SLVVEASGGEAAAKLYKQSDEKGFQASETEVLKQLSSMGRLVVCAGSGAVQSSTNL---A 327

Query: 241 MLNFPGSVHTGTFEAWHIIMDDLPLQMIAEEFAEDGSQLPVLDISTSGSYSEVLGQITSL 300
           +L      H  T      +  DLPLQMIAEEFAED SQ PV DISTSGSYSEVLGQITS+
Sbjct: 328 LLR-----HGIT------LWIDLPLQMIAEEFAEDRSQHPVFDISTSGSYSEVLGQITSI 387

Query: 301 YQEIKDGYATADASISLQKLASELGYDDLNAVTTEDMALEALKELEKLTRVKKMIEAAAK 360
           YQ++KDGYATADA ISLQKLAS+LGYDD NAVTTEDMA+EALKE+EKLTRVKKMIEAAAK
Sbjct: 388 YQKVKDGYATADALISLQKLASKLGYDDFNAVTTEDMAMEALKEIEKLTRVKKMIEAAAK 394

Query: 361 PF 363
           PF
Sbjct: 448 PF 394

BLAST of Sgr016575 vs. ExPASy TrEMBL
Match: A0A1S3BNN4 (probable inactive shikimate kinase like 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103491834 PE=3 SV=1)

HSP 1 Score: 356.3 bits (913), Expect = 1.5e-94
Identity = 226/362 (62.43%), Postives = 254/362 (70.17%), Query Frame = 0

Query: 1   MAMKLKTTVSSARPDLQFYVRLLNVQSMALNLNCSLPLPSSLRRKHNLPLHVRRRFLDSS 60
           MAM LKTTVS    DLQF + L   ++MALN + SL LPSSLRR H+LPLH+   F+ SS
Sbjct: 1   MAMNLKTTVSPL-TDLQFNIHLPKFEAMALNSSFSLSLPSSLRRNHSLPLHICPPFIQSS 60

Query: 61  ANRRRRSLPTTCAVSDGTAFSEFKFGIHPLPQDDLSWALLDVEAKVTVDDLSLEVKKKAM 120
           A RRRRSLP++C VSDGT+                    L+VEAKVTVDDLSLEVKKKAM
Sbjct: 61  AIRRRRSLPSSCLVSDGTS--------------------LNVEAKVTVDDLSLEVKKKAM 120

Query: 121 DVAPELKELPSFSWVSNNLQFWRNFGSYRDAFSFWACGNVFLPNLLILNFYMSHANAYSD 180
           DV PELK    F  V  N     N G               L ++L   ++       SD
Sbjct: 121 DVGPELKGTSIF-LVGINSSIKTNLGK-------------LLADVLRYYYF------DSD 180

Query: 181 SLVVEASGGAAAAKLYKESDENGFRASETEVLNQLSSMGRLVICAGNGAVQSSTNLYERS 240
           SLVVEASGG AAAKLYK+SDE GF+ASETEVL QLSSMGRLV+CAG+GAVQSSTNL   +
Sbjct: 181 SLVVEASGGEAAAKLYKQSDEKGFQASETEVLKQLSSMGRLVVCAGSGAVQSSTNL---A 240

Query: 241 MLNFPGSVHTGTFEAWHIIMDDLPLQMIAEEFAEDGSQLPVLDISTSGSYSEVLGQITSL 300
           +L      H  T      +  DLPLQMIAEEFAED SQ PV DISTSGSYSEVLGQITS+
Sbjct: 241 LLR-----HGIT------LWIDLPLQMIAEEFAEDRSQHPVFDISTSGSYSEVLGQITSI 300

Query: 301 YQEIKDGYATADASISLQKLASELGYDDLNAVTTEDMALEALKELEKLTRVKKMIEAAAK 360
           YQ++KDGYATADA ISLQKLAS+LGYDD NAVTTEDMA+EALKE+EKLTRVKKMIEAAAK
Sbjct: 301 YQKVKDGYATADALISLQKLASKLGYDDFNAVTTEDMAMEALKEIEKLTRVKKMIEAAAK 307

Query: 361 PF 363
           PF
Sbjct: 361 PF 307

BLAST of Sgr016575 vs. ExPASy TrEMBL
Match: A0A6J1JQ24 (probable inactive shikimate kinase like 1, chloroplastic isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111486699 PE=4 SV=1)

HSP 1 Score: 351.7 bits (901), Expect = 3.7e-93
Identity = 223/360 (61.94%), Postives = 246/360 (68.33%), Query Frame = 0

Query: 3   MKLKTTVSSARPDLQFYVRLLNVQSMALNLNCSLPLPSSLRRKHNLPLHVRRRFLDSSAN 62
           M +KTTVS    D QF + L  +Q M+LN + +LPLP S+R   NLPLH RRRFL SS  
Sbjct: 1   MAMKTTVSPL-TDFQFNIHLPKIQPMSLNSSYALPLPFSVRCNQNLPLHFRRRFLHSSVI 60

Query: 63  RRRRSLPTTCAVSDGTAFSEFKFGIHPLPQDDLSWALLDVEAKVTVDDLSLEVKKKAMDV 122
           +RRRSLP+TC VSDGTA+                    +VEAK T DDLSLEVKKKAMDV
Sbjct: 61  QRRRSLPSTCFVSDGTAY--------------------NVEAKATTDDLSLEVKKKAMDV 120

Query: 123 APELKELPSFSWVSNNLQFWRNFGSYRDAFSFWACGNVFLPNLLILNFYMSHANAYSDSL 182
           APELK    F  V  N       G               L ++  L +Y       SD L
Sbjct: 121 APELKGTSIF-LVGINSSLKTKLGK-------------LLADM--LRYYYFDRYFLSDGL 180

Query: 183 VVEASGGAAAAKLYKESDENGFRASETEVLNQLSSMGRLVICAGNGAVQSSTNLYERSML 242
           VVEASGG AAAKLYKESDENGFRASETEVL QLSSMGRLV+CAGNGAV+SSTNL   ++L
Sbjct: 181 VVEASGGVAAAKLYKESDENGFRASETEVLKQLSSMGRLVVCAGNGAVRSSTNL---ALL 240

Query: 243 NFPGSVHTGTFEAWHIIMDDLPLQMIAEEFAEDGSQLPVLDISTSGSYSEVLGQITSLYQ 302
                 H  T      +  D+PLQ+IAEEFAED  QLPV DISTSGSYSEVLGQITSLYQ
Sbjct: 241 R-----HGIT------LWIDIPLQIIAEEFAEDRGQLPVFDISTSGSYSEVLGQITSLYQ 300

Query: 303 EIKDGYATADASISLQKLASELGYDDLNAVTTEDMALEALKELEKLTRVKKMIEAAAKPF 362
           EIK GYATADASISLQKLAS LGYDD NAVTTEDMALEALKE+EKL RVKKM+EAAAKPF
Sbjct: 301 EIKVGYATADASISLQKLASTLGYDDFNAVTTEDMALEALKEIEKLIRVKKMMEAAAKPF 309

BLAST of Sgr016575 vs. ExPASy TrEMBL
Match: A0A6J1JN35 (probable inactive shikimate kinase like 1, chloroplastic isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111486699 PE=3 SV=1)

HSP 1 Score: 349.0 bits (894), Expect = 2.4e-92
Identity = 222/360 (61.67%), Postives = 246/360 (68.33%), Query Frame = 0

Query: 3   MKLKTTVSSARPDLQFYVRLLNVQSMALNLNCSLPLPSSLRRKHNLPLHVRRRFLDSSAN 62
           M +KTTVS    D QF + L  +Q M+LN + +LPLP S+R   NLPLH RRRFL SS  
Sbjct: 1   MAMKTTVSPL-TDFQFNIHLPKIQPMSLNSSYALPLPFSVRCNQNLPLHFRRRFLHSSVI 60

Query: 63  RRRRSLPTTCAVSDGTAFSEFKFGIHPLPQDDLSWALLDVEAKVTVDDLSLEVKKKAMDV 122
           +RRRSLP+TC VSDGTA+                    +VEAK T DDLSLEVKKKAMDV
Sbjct: 61  QRRRSLPSTCFVSDGTAY--------------------NVEAKATTDDLSLEVKKKAMDV 120

Query: 123 APELKELPSFSWVSNNLQFWRNFGSYRDAFSFWACGNVFLPNLLILNFYMSHANAYSDSL 182
           APELK    F  V  N       G               L ++L   ++       SD L
Sbjct: 121 APELKGTSIF-LVGINSSLKTKLGK-------------LLADMLRYYYF------DSDGL 180

Query: 183 VVEASGGAAAAKLYKESDENGFRASETEVLNQLSSMGRLVICAGNGAVQSSTNLYERSML 242
           VVEASGG AAAKLYKESDENGFRASETEVL QLSSMGRLV+CAGNGAV+SSTNL   ++L
Sbjct: 181 VVEASGGVAAAKLYKESDENGFRASETEVLKQLSSMGRLVVCAGNGAVRSSTNL---ALL 240

Query: 243 NFPGSVHTGTFEAWHIIMDDLPLQMIAEEFAEDGSQLPVLDISTSGSYSEVLGQITSLYQ 302
                 H  T      +  D+PLQ+IAEEFAED  QLPV DISTSGSYSEVLGQITSLYQ
Sbjct: 241 R-----HGIT------LWIDIPLQIIAEEFAEDRGQLPVFDISTSGSYSEVLGQITSLYQ 300

Query: 303 EIKDGYATADASISLQKLASELGYDDLNAVTTEDMALEALKELEKLTRVKKMIEAAAKPF 362
           EIK GYATADASISLQKLAS LGYDD NAVTTEDMALEALKE+EKL RVKKM+EAAAKPF
Sbjct: 301 EIKVGYATADASISLQKLASTLGYDDFNAVTTEDMALEALKEIEKLIRVKKMMEAAAKPF 305

BLAST of Sgr016575 vs. TAIR 10
Match: AT3G26900.1 (shikimate kinase like 1 )

HSP 1 Score: 146.4 bits (368), Expect = 4.6e-35
Identity = 105/248 (42.34%), Postives = 143/248 (57.66%), Query Frame = 0

Query: 115 VKKKAMDVAPELKELPSFSWVSNNLQFWRNFGSYRDAFSFWACGNVFLPNLLILNFYMSH 174
           VK+KAMD+APELK    F  V  N     N G                    +L   + +
Sbjct: 76  VKRKAMDIAPELKGASIF-LVGINNSIKTNTGK-------------------LLAEALRY 135

Query: 175 ANAYSDSLVVEASGGAAAAKLYKESDENGFRASETEVLNQLSSMGRLVICAGNGAVQSST 234
               SD+L+ EA+GG  +A+  KE+DE  F+ SETEVL QLSSMGRLV+CAG+GAVQS  
Sbjct: 136 YYFDSDNLITEAAGGNVSAQALKEADEKAFQESETEVLKQLSSMGRLVVCAGDGAVQSLR 195

Query: 235 NLYERSMLNFPGSVHTGTFEAWHIIMDDLPLQMIAEEFAEDGSQLPVLDISTSGSYSEVL 294
           NL   ++L    S           I  D+PL + A+   +D       D   S    E+ 
Sbjct: 196 NL---ALLRHGIS-----------IWIDVPLDITAK--GDD-------DSFHSEPSPELF 255

Query: 295 GQITSLYQEIKDGYATADASISLQKLASELGYDDLNAVTTEDMALEALKELEKLTRVKKM 354
             + + Y++ + GY TAD SISL+K+A++L ++DL AVT+ED+ALE LKE+EKLTRVKKM
Sbjct: 256 DTLKASYEKSRKGYETADVSISLEKIATKLEFEDLEAVTSEDIALEILKEIEKLTRVKKM 280

Query: 355 IEAAAKPF 363
           +E A++PF
Sbjct: 316 MEEASRPF 280

BLAST of Sgr016575 vs. TAIR 10
Match: AT3G26900.2 (shikimate kinase like 1 )

HSP 1 Score: 146.4 bits (368), Expect = 4.6e-35
Identity = 105/248 (42.34%), Postives = 143/248 (57.66%), Query Frame = 0

Query: 115 VKKKAMDVAPELKELPSFSWVSNNLQFWRNFGSYRDAFSFWACGNVFLPNLLILNFYMSH 174
           VK+KAMD+APELK    F  V  N     N G                    +L   + +
Sbjct: 76  VKRKAMDIAPELKGASIF-LVGINNSIKTNTGK-------------------LLAEALRY 135

Query: 175 ANAYSDSLVVEASGGAAAAKLYKESDENGFRASETEVLNQLSSMGRLVICAGNGAVQSST 234
               SD+L+ EA+GG  +A+  KE+DE  F+ SETEVL QLSSMGRLV+CAG+GAVQS  
Sbjct: 136 YYFDSDNLITEAAGGNVSAQALKEADEKAFQESETEVLKQLSSMGRLVVCAGDGAVQSLR 195

Query: 235 NLYERSMLNFPGSVHTGTFEAWHIIMDDLPLQMIAEEFAEDGSQLPVLDISTSGSYSEVL 294
           NL   ++L    S           I  D+PL + A+   +D       D   S    E+ 
Sbjct: 196 NL---ALLRHGIS-----------IWIDVPLDITAK--GDD-------DSFHSEPSPELF 255

Query: 295 GQITSLYQEIKDGYATADASISLQKLASELGYDDLNAVTTEDMALEALKELEKLTRVKKM 354
             + + Y++ + GY TAD SISL+K+A++L ++DL AVT+ED+ALE LKE+EKLTRVKKM
Sbjct: 256 DTLKASYEKSRKGYETADVSISLEKIATKLEFEDLEAVTSEDIALEILKEIEKLTRVKKM 280

Query: 355 IEAAAKPF 363
           +E A++PF
Sbjct: 316 MEEASRPF 280

BLAST of Sgr016575 vs. TAIR 10
Match: AT3G26900.3 (shikimate kinase like 1 )

HSP 1 Score: 146.4 bits (368), Expect = 4.6e-35
Identity = 105/248 (42.34%), Postives = 143/248 (57.66%), Query Frame = 0

Query: 115 VKKKAMDVAPELKELPSFSWVSNNLQFWRNFGSYRDAFSFWACGNVFLPNLLILNFYMSH 174
           VK+KAMD+APELK    F  V  N     N G                    +L   + +
Sbjct: 76  VKRKAMDIAPELKGASIF-LVGINNSIKTNTGK-------------------LLAEALRY 135

Query: 175 ANAYSDSLVVEASGGAAAAKLYKESDENGFRASETEVLNQLSSMGRLVICAGNGAVQSST 234
               SD+L+ EA+GG  +A+  KE+DE  F+ SETEVL QLSSMGRLV+CAG+GAVQS  
Sbjct: 136 YYFDSDNLITEAAGGNVSAQALKEADEKAFQESETEVLKQLSSMGRLVVCAGDGAVQSLR 195

Query: 235 NLYERSMLNFPGSVHTGTFEAWHIIMDDLPLQMIAEEFAEDGSQLPVLDISTSGSYSEVL 294
           NL   ++L    S           I  D+PL + A+   +D       D   S    E+ 
Sbjct: 196 NL---ALLRHGIS-----------IWIDVPLDITAK--GDD-------DSFHSEPSPELF 255

Query: 295 GQITSLYQEIKDGYATADASISLQKLASELGYDDLNAVTTEDMALEALKELEKLTRVKKM 354
             + + Y++ + GY TAD SISL+K+A++L ++DL AVT+ED+ALE LKE+EKLTRVKKM
Sbjct: 256 DTLKASYEKSRKGYETADVSISLEKIATKLEFEDLEAVTSEDIALEILKEIEKLTRVKKM 280

Query: 355 IEAAAKPF 363
           +E A++PF
Sbjct: 316 MEEASRPF 280