Sgr016489 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr016489 vs. NCBI nr
Match: XP_022138430.1 (protein SIEVE ELEMENT OCCLUSION B-like [Momordica charantia])

HSP 1 Score: 1175.2 bits (3039), Expect = 0.0e+00
Identity = 579/686 (84.40%), Postives = 624/686 (90.96%), Query Frame = 0

Query: 1   MSAPPPKTPTAPLALQTAKEERTLKHFSDDVITHHIYTKHSDDETTKIDLNNYISFIENV 60
           MS P PKTP APLAL  A EER+LK+FSDDVIT HIYTKHSDDE  KIDLNNYISFIENV
Sbjct: 1   MSTPRPKTPAAPLAL-AANEERSLKYFSDDVITSHIYTKHSDDEAMKIDLNNYISFIENV 60

Query: 61  IKTADQIAETHYG-AQGSKGHLVFSDDSLKYLSSSIDPPICTLHQISKELACKAPGIEGA 120
           +KTADQIAETH G  QGSKGH VFSDD LKY  SSIDPP+CTLHQISKELACKAPGIE A
Sbjct: 61  VKTADQIAETHLGRVQGSKGHFVFSDDPLKY-PSSIDPPLCTLHQISKELACKAPGIETA 120

Query: 121 HKTTLDILSKLTSYPWEAKAALTFTAFATDYGVMWHLEHYSQSDPLAKSLAMIKRVALLK 180
           HKTTLDIL+KL SYPWEAKAALTF AFATDYGV+WHL+HYS SDPLAKSLAMIKRVA LK
Sbjct: 121 HKTTLDILNKLISYPWEAKAALTFAAFATDYGVIWHLDHYSHSDPLAKSLAMIKRVAWLK 180

Query: 181 KELGSFKYRHVFFDPNSLIYSCLQAMKYINELKNLSKYDTKEVPELSAAVRQIPLVSYWV 240
           KEL SFKYRHV    NSLIYSCLQA+K INELKN+SKYDTKEVPELSAA+RQIPLVSYW+
Sbjct: 181 KELESFKYRHV----NSLIYSCLQAIKNINELKNISKYDTKEVPELSAALRQIPLVSYWI 240

Query: 241 IHTLVASSTELHSYLSGTDGQTQKYLNELAEKFRSLLFTLEKHLQFIREQQEEVELYRWL 300
           IHTLVASSTELHSYLSG +GQT KYLNEL EKFRSLLFT EK LQFIREQQEEVELY+ L
Sbjct: 241 IHTLVASSTELHSYLSGAEGQTLKYLNELDEKFRSLLFTFEKQLQFIREQQEEVELYKSL 300

Query: 301 VDQTDHFPTDAALFLAKLIDGKLKPKPLINCSTQLEENIEEFLKEKKLILVVSKRLDVSS 360
           V QTDHFPTD  LFL+KLIDGK KP+PLINCSTQLEE+IEEFLKEK LILVVSK L  S 
Sbjct: 301 VGQTDHFPTDVTLFLSKLIDGKHKPQPLINCSTQLEEHIEEFLKEKNLILVVSKGLKFSM 360

Query: 361 EDLEILHLIYNELKKENKYEMVWIPVISDPPNEGDEEAYKHLKSKMKWYVVPFTTKVAGV 420
           EDLEILH+IY+ELK+ENKYE+VW+PVISDPPNEGDEEAY+ LKSKMKWY +PFTTKVAGV
Sbjct: 361 EDLEILHVIYDELKRENKYEIVWVPVISDPPNEGDEEAYESLKSKMKWYALPFTTKVAGV 420

Query: 421 RFLEEKWQLREDLLMVVLDSKSKIEFTNAIHLTRVWERDAIPLTYERAKALLRRNWIEST 480
           RFLEEKWQLREDL++VVLDSKSKIEFTNA+HLTRVW+R+AIP TYERAKALLR+NWI+ST
Sbjct: 421 RFLEEKWQLREDLMVVVLDSKSKIEFTNAVHLTRVWKREAIPFTYERAKALLRKNWIDST 480

Query: 481 VVKFSDQPRLQSWINQERNIIFYGGHNSKWVRQFEETAEAIKKDPWTREEGITFEMIPIG 540
           VVKFSDQPRL+SWINQERNIIFYGGHN  WVR+FEETAEAIK+DP  REEG TFEM+PIG
Sbjct: 481 VVKFSDQPRLRSWINQERNIIFYGGHNPNWVRKFEETAEAIKRDPLAREEGATFEMVPIG 540

Query: 541 RRDKGEYDPAMMFRFWMAQRSFFIIKHQLHGSNASEDVSRLISYENEHGWAIIAKGPTVV 600
           R DKGE+DPA+MFRFWM QRSFF IKHQLHGS+ASEDVS+LISYENE+GWAII KGPTV+
Sbjct: 541 RNDKGEHDPAVMFRFWMVQRSFFNIKHQLHGSSASEDVSQLISYENENGWAIIVKGPTVI 600

Query: 601 FIGGGDLILKAMEEFQTWKKNLRRGGFFSSFKDYFDVLTAKSIHCTHVNILGFSGWIPLI 660
           F+GGGDLILKAMEEFQ WKKNLR  GF +SFKDY+  LTAKS HCTHVNILGFSGWIPLI
Sbjct: 601 FVGGGDLILKAMEEFQAWKKNLRWEGFSASFKDYYHELTAKSPHCTHVNILGFSGWIPLI 660

Query: 661 IICPVCRRYMGSGIRFTCCHGGPDVL 686
           I+CP+C RYMGSGIRFTCCHGGPDVL
Sbjct: 661 IVCPMCHRYMGSGIRFTCCHGGPDVL 680

BLAST of Sgr016489 vs. NCBI nr
Match: XP_022958758.1 (protein SIEVE ELEMENT OCCLUSION B-like [Cucurbita moschata])

HSP 1 Score: 1066.2 bits (2756), Expect = 1.2e-307
Identity = 510/691 (73.81%), Postives = 599/691 (86.69%), Query Frame = 0

Query: 1   MSAPPPKTPTAPLAL----QTAKEERTLKHFSDDVITHHIYTKHSDDETTKIDLNNYISF 60
           MS   PK+  A LAL    QT KEE  LKH SDDVIT+ I+   +D+E TKIDL+NY+ F
Sbjct: 1   MSLLRPKSSAAALALALSQQTDKEEPGLKHLSDDVITNRIFDFTTDEEMTKIDLDNYVLF 60

Query: 61  IENVIKTADQIAETHYGAQGSKGHLVFSDDSLKYLSSSIDPPICTLHQISKELACKAPGI 120
           IENV+K +DQI    + AQGSKGH V ++D++ Y  S IDPPICTLHQIS E+ACK PG+
Sbjct: 61  IENVLKASDQICVASHWAQGSKGHFVLAEDTMNY-PSPIDPPICTLHQISSEMACKPPGV 120

Query: 121 EGAHKTTLDILSKLTSYPWEAKAALTFTAFATDYGVMWHLEHYSQSDPLAKSLAMIKRVA 180
           E  HKTTLDILSKLT YPWEAKA L FTAF T+YGV+WHL+++S SDPLAKSLAMIKRV 
Sbjct: 121 ETVHKTTLDILSKLTRYPWEAKAVLIFTAFVTNYGVLWHLDNHSHSDPLAKSLAMIKRVG 180

Query: 181 LLKKELGSFKYRHVFFDPNSLIYSCLQAMKYINELKNLSKYDTKEVPELSAAVRQIPLVS 240
           LL+KEL S KY  VFF+ NS+IY+CL+A+KYINE K+LSKYDTK+VPELSAA+RQIPLVS
Sbjct: 181 LLRKELESVKYGQVFFNQNSMIYNCLKAVKYINEFKSLSKYDTKDVPELSAALRQIPLVS 240

Query: 241 YWVIHTLVASSTELHSYLSGTDGQTQKYLNELAEKFRSLLFTLEKHLQFIREQQEEVELY 300
           YW+IH LVASS ELH YLSG +GQTQKYLNE+ EK  SL+ TLE H+QFIREQQEEVELY
Sbjct: 241 YWIIHILVASSIELHCYLSGVEGQTQKYLNEMTEKVSSLVLTLENHMQFIREQQEEVELY 300

Query: 301 RWLVDQTDHFPTDAALFLAKLIDGKLKPKPLINCSTQLEENIEEFLKEKKLILVVSKRLD 360
           RWLVDQTDHFPTD  LFLAKLI+GK K +PLINCSTQLEE+IEEF+KEKKLIL+VS+ L+
Sbjct: 301 RWLVDQTDHFPTDVTLFLAKLINGKHKARPLINCSTQLEEHIEEFMKEKKLILIVSRSLN 360

Query: 361 VSSEDLEILHLIYNELKKENKYEMVWIPVISDPPNEGDEEAYKHLKSKMKWYVVPFTTKV 420
            S EDLEIL  +Y E+KKENK+EMVWIPVISDPPN+GDEEAY+ LKS+MKW+VVPF  K+
Sbjct: 361 FSREDLEILQSVYKEVKKENKFEMVWIPVISDPPNDGDEEAYEALKSEMKWFVVPFAAKI 420

Query: 421 AGVRFLEEKWQLREDLLMVVLDSKSKIEFTNAIHLTRVWERDAIPLTYERAKALLRRNWI 480
           AGVRFLEE+W+LREDLL+VVLD++SK+EF+NAIHLTRVWE++AIP +Y+R KAL+++NWI
Sbjct: 421 AGVRFLEERWELREDLLLVVLDTQSKVEFSNAIHLTRVWEKEAIPFSYDRVKALMKKNWI 480

Query: 481 ESTVVKFSDQPRLQSW--INQERNIIFYGGHNSKWVRQFEETAEAIKKDPWTREEGITFE 540
           +STV+K++DQPRL+S   INQERN+IFYGGHN +WV+ FE++AEA+K+DP TREEGITFE
Sbjct: 481 DSTVLKYTDQPRLKSLVVINQERNVIFYGGHNPRWVKNFEDSAEAMKRDPVTREEGITFE 540

Query: 541 MIPIGRRDKGEYDPAMMFRFWMAQRSFFIIKHQLHGSNASEDVSRLISYENEHGWAIIAK 600
           ++P+GR +KGE+DPA+MF FWMAQRS+FI+KHQLHGS ASED+SRLISYE E GWAII K
Sbjct: 541 LVPVGRNNKGEHDPAVMFNFWMAQRSYFILKHQLHGSTASEDISRLISYETEKGWAIITK 600

Query: 601 GPTVVFIGGGDLILKAMEEFQTWKKNLRRGGFFSSFKDYFDVLTAKSIHCTHVNILGFSG 660
           GP VVFIGGGDLILKA++EFQ WKKNLRR GF  SFKDYFD LTA S+HCTHVNI+GFSG
Sbjct: 601 GPIVVFIGGGDLILKAIDEFQVWKKNLRRVGFSGSFKDYFDELTASSLHCTHVNIIGFSG 660

Query: 661 WIPLIIICPVCRRYMGSGIRFTCCHGGPDVL 686
           WIPLII CPVCRRYMGSGIRFTCCHGGPDVL
Sbjct: 661 WIPLIITCPVCRRYMGSGIRFTCCHGGPDVL 690

BLAST of Sgr016489 vs. NCBI nr
Match: XP_023548586.1 (protein SIEVE ELEMENT OCCLUSION B-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1066.2 bits (2756), Expect = 1.2e-307
Identity = 510/691 (73.81%), Postives = 599/691 (86.69%), Query Frame = 0

Query: 1   MSAPPPKTPTAPLAL----QTAKEERTLKHFSDDVITHHIYTKHSDDETTKIDLNNYISF 60
           MS   PK+  A LAL    QT KEE  LKH SDDVIT+ I+   +D+E TKIDL+NY+ F
Sbjct: 1   MSLLRPKSSAAALALALSQQTDKEEPGLKHLSDDVITNRIFDFMTDEEMTKIDLDNYVLF 60

Query: 61  IENVIKTADQIAETHYGAQGSKGHLVFSDDSLKYLSSSIDPPICTLHQISKELACKAPGI 120
           IENV+K +DQI    + AQGSKGH V ++D++ Y  S IDPPICTLHQIS E+ACK PG+
Sbjct: 61  IENVLKASDQICVASHWAQGSKGHFVLAEDTMNY-PSPIDPPICTLHQISSEMACKPPGV 120

Query: 121 EGAHKTTLDILSKLTSYPWEAKAALTFTAFATDYGVMWHLEHYSQSDPLAKSLAMIKRVA 180
           E  HKTTLDILSKLT YPWEAKA L FTAF T+YGV+WHL+++S SDPLAKSLA IKRV 
Sbjct: 121 ETVHKTTLDILSKLTRYPWEAKAVLIFTAFVTNYGVLWHLDNHSHSDPLAKSLATIKRVG 180

Query: 181 LLKKELGSFKYRHVFFDPNSLIYSCLQAMKYINELKNLSKYDTKEVPELSAAVRQIPLVS 240
           LL+KEL S KY  VFF+ NS+IY+CL+A+KYINE K+LSKYDTK+VPELSAA+RQIPLVS
Sbjct: 181 LLRKELDSVKYGQVFFNQNSMIYNCLKAVKYINEFKSLSKYDTKDVPELSAALRQIPLVS 240

Query: 241 YWVIHTLVASSTELHSYLSGTDGQTQKYLNELAEKFRSLLFTLEKHLQFIREQQEEVELY 300
           YW+IH LVASS ELH YLSG +GQTQKYLNE+ EK  SL+ TLE H+QFIREQQEEVELY
Sbjct: 241 YWIIHILVASSIELHCYLSGVEGQTQKYLNEMTEKVSSLVLTLENHMQFIREQQEEVELY 300

Query: 301 RWLVDQTDHFPTDAALFLAKLIDGKLKPKPLINCSTQLEENIEEFLKEKKLILVVSKRLD 360
           RWLVDQTDHFPTD  LFLAKLI+GK K +PLINCSTQLEE+IEEF+KEKKLIL+VS+ L+
Sbjct: 301 RWLVDQTDHFPTDVTLFLAKLINGKHKARPLINCSTQLEEHIEEFMKEKKLILIVSRSLN 360

Query: 361 VSSEDLEILHLIYNELKKENKYEMVWIPVISDPPNEGDEEAYKHLKSKMKWYVVPFTTKV 420
            S EDLEIL  +Y E+KKENK+EMVWIPVISDPPN+GDEEAY+ LKS+MKW+VVPF TK+
Sbjct: 361 FSREDLEILQSVYKEVKKENKFEMVWIPVISDPPNDGDEEAYEALKSEMKWFVVPFDTKI 420

Query: 421 AGVRFLEEKWQLREDLLMVVLDSKSKIEFTNAIHLTRVWERDAIPLTYERAKALLRRNWI 480
           AGVRFLEE+W+LREDLL+VVLD++SK+EF+NAIHLTRVWE++AIP +Y+R KAL+++NWI
Sbjct: 421 AGVRFLEERWELREDLLLVVLDTQSKVEFSNAIHLTRVWEKEAIPFSYDRVKALMKKNWI 480

Query: 481 ESTVVKFSDQPRLQSW--INQERNIIFYGGHNSKWVRQFEETAEAIKKDPWTREEGITFE 540
           +STV+KF+DQPRL+S   INQERN+IFYGGHN +WV+ FE++AEA+K+DP TREEGITFE
Sbjct: 481 DSTVLKFTDQPRLKSLVVINQERNVIFYGGHNPRWVKNFEDSAEAMKRDPVTREEGITFE 540

Query: 541 MIPIGRRDKGEYDPAMMFRFWMAQRSFFIIKHQLHGSNASEDVSRLISYENEHGWAIIAK 600
           ++P+GR +KGE+DP +MF FWMAQRS+FI+KHQLHGS ASED+SRLISYE E GWAII K
Sbjct: 541 LVPVGRNNKGEHDPTVMFNFWMAQRSYFILKHQLHGSTASEDISRLISYETEKGWAIITK 600

Query: 601 GPTVVFIGGGDLILKAMEEFQTWKKNLRRGGFFSSFKDYFDVLTAKSIHCTHVNILGFSG 660
           GPTVVFIGGGDLILKA++EFQ WKKNLRR GF  S+KDYFD LTA S+HCTHVNI+GFSG
Sbjct: 601 GPTVVFIGGGDLILKAIDEFQIWKKNLRRVGFSGSYKDYFDELTASSLHCTHVNIIGFSG 660

Query: 661 WIPLIIICPVCRRYMGSGIRFTCCHGGPDVL 686
           WIPLII CPVCRRYMGSGIRFTCCHGGPDVL
Sbjct: 661 WIPLIITCPVCRRYMGSGIRFTCCHGGPDVL 690

BLAST of Sgr016489 vs. NCBI nr
Match: KAG7013505.1 (Protein SIEVE ELEMENT OCCLUSION B [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1062.8 bits (2747), Expect = 1.3e-306
Identity = 508/691 (73.52%), Postives = 598/691 (86.54%), Query Frame = 0

Query: 1   MSAPPPKTPTAPLAL----QTAKEERTLKHFSDDVITHHIYTKHSDDETTKIDLNNYISF 60
           MS   PK+    LAL    QT KEE  LKH SDDVIT+ I+   +D+E TKIDL+NY+ F
Sbjct: 1   MSLLRPKSSATALALALSQQTDKEEPGLKHLSDDVITNRIFDFMTDEEMTKIDLDNYVLF 60

Query: 61  IENVIKTADQIAETHYGAQGSKGHLVFSDDSLKYLSSSIDPPICTLHQISKELACKAPGI 120
           IENV+K +DQI    + AQGSKGH V ++D++ Y  S IDPPICTLHQIS E+ACK PG+
Sbjct: 61  IENVLKASDQICVASHWAQGSKGHFVLAEDTMNY-PSPIDPPICTLHQISSEMACKPPGV 120

Query: 121 EGAHKTTLDILSKLTSYPWEAKAALTFTAFATDYGVMWHLEHYSQSDPLAKSLAMIKRVA 180
           E  HKTTL+ILSKLT YPWEAKA L FTAF T+YGV+WHL+++S SDPLAKSLA IKRVA
Sbjct: 121 ETVHKTTLNILSKLTRYPWEAKAVLIFTAFVTNYGVLWHLDNHSHSDPLAKSLATIKRVA 180

Query: 181 LLKKELGSFKYRHVFFDPNSLIYSCLQAMKYINELKNLSKYDTKEVPELSAAVRQIPLVS 240
           LL+KEL S KY  VFF+ NS+IY+CL+A+KYINE K+LSKYDTK+VPELSAA+RQIPLVS
Sbjct: 181 LLRKELDSVKYGQVFFNQNSMIYNCLKAVKYINEFKSLSKYDTKDVPELSAALRQIPLVS 240

Query: 241 YWVIHTLVASSTELHSYLSGTDGQTQKYLNELAEKFRSLLFTLEKHLQFIREQQEEVELY 300
           YW+IH LVASS ELH YLSG +GQTQKYLNE+ EK  SL+ TLE H+QFIREQQEEVELY
Sbjct: 241 YWIIHILVASSIELHCYLSGVEGQTQKYLNEMTEKVSSLVLTLENHMQFIREQQEEVELY 300

Query: 301 RWLVDQTDHFPTDAALFLAKLIDGKLKPKPLINCSTQLEENIEEFLKEKKLILVVSKRLD 360
           RWLVDQTDHFPTD  LFLAKLI+GK K +PLINCSTQLEE+IEEF+KEKKLIL+VS+ L+
Sbjct: 301 RWLVDQTDHFPTDVTLFLAKLINGKHKARPLINCSTQLEEHIEEFMKEKKLILIVSRSLN 360

Query: 361 VSSEDLEILHLIYNELKKENKYEMVWIPVISDPPNEGDEEAYKHLKSKMKWYVVPFTTKV 420
            S EDLEIL  +Y E+KKENK+EMVWIPVISDPPN+GDEEAY+ LKS+MKW+VVPF  K+
Sbjct: 361 FSREDLEILQSVYKEVKKENKFEMVWIPVISDPPNDGDEEAYEALKSEMKWFVVPFAAKI 420

Query: 421 AGVRFLEEKWQLREDLLMVVLDSKSKIEFTNAIHLTRVWERDAIPLTYERAKALLRRNWI 480
           AGVRFLEE+W+LREDLL+VVLD++SK+EF+NAIHLTRVWE++AIP +Y+R KAL+++NWI
Sbjct: 421 AGVRFLEERWELREDLLLVVLDTQSKVEFSNAIHLTRVWEKEAIPFSYDRVKALMKKNWI 480

Query: 481 ESTVVKFSDQPRLQSW--INQERNIIFYGGHNSKWVRQFEETAEAIKKDPWTREEGITFE 540
           +STV+K++DQPRL+S   INQERN+IFYGGHN +WV+ FE++AEA+K+DP TREEGITFE
Sbjct: 481 DSTVLKYTDQPRLKSLVVINQERNVIFYGGHNPRWVKNFEDSAEAMKRDPVTREEGITFE 540

Query: 541 MIPIGRRDKGEYDPAMMFRFWMAQRSFFIIKHQLHGSNASEDVSRLISYENEHGWAIIAK 600
           ++P+GR +KGE+DPA+MF FWMAQRS+FI+KHQLHGS ASED+SRLISYE E GWAII K
Sbjct: 541 LVPVGRNNKGEHDPAVMFNFWMAQRSYFILKHQLHGSTASEDISRLISYETEKGWAIITK 600

Query: 601 GPTVVFIGGGDLILKAMEEFQTWKKNLRRGGFFSSFKDYFDVLTAKSIHCTHVNILGFSG 660
           GPTVVFIGGGDLILKA++EFQ WKKNLRR GF  SFKDYFD LTA  +HCTHVNI+GFSG
Sbjct: 601 GPTVVFIGGGDLILKAIDEFQIWKKNLRRVGFSGSFKDYFDELTASGLHCTHVNIIGFSG 660

Query: 661 WIPLIIICPVCRRYMGSGIRFTCCHGGPDVL 686
           WIPLII CPVCRRYMGSGIRFTCCHGGPDVL
Sbjct: 661 WIPLIITCPVCRRYMGSGIRFTCCHGGPDVL 690

BLAST of Sgr016489 vs. NCBI nr
Match: KAG6574937.1 (Protein SIEVE ELEMENT OCCLUSION B, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1062.4 bits (2746), Expect = 1.7e-306
Identity = 508/691 (73.52%), Postives = 598/691 (86.54%), Query Frame = 0

Query: 1   MSAPPPKTPTAPLAL----QTAKEERTLKHFSDDVITHHIYTKHSDDETTKIDLNNYISF 60
           MS   PK+    LAL    QT KEE  LKH SDDVIT+ I+   +D+E TKIDL+NY+ F
Sbjct: 1   MSLLRPKSSATALALALSQQTDKEEPGLKHLSDDVITNRIFDFMTDEEMTKIDLDNYVLF 60

Query: 61  IENVIKTADQIAETHYGAQGSKGHLVFSDDSLKYLSSSIDPPICTLHQISKELACKAPGI 120
           IENV+K +DQI    + AQGSKGH V ++D++ Y  S IDPPICTLHQIS E+ACK PG+
Sbjct: 61  IENVLKASDQICVASHWAQGSKGHFVLAEDTMNY-PSPIDPPICTLHQISSEMACKPPGV 120

Query: 121 EGAHKTTLDILSKLTSYPWEAKAALTFTAFATDYGVMWHLEHYSQSDPLAKSLAMIKRVA 180
           E  HKTTLDILSKLT YPWEAKA L FTAF T+YGV+WHL+++S SDPLAKSLA IKRVA
Sbjct: 121 ETVHKTTLDILSKLTRYPWEAKAVLIFTAFVTNYGVLWHLDNHSHSDPLAKSLATIKRVA 180

Query: 181 LLKKELGSFKYRHVFFDPNSLIYSCLQAMKYINELKNLSKYDTKEVPELSAAVRQIPLVS 240
           LL+KEL S KY  VFF+ NS+IY+CL+A+KYINE K+LSKYDTK+VPELSAA+RQIPLVS
Sbjct: 181 LLRKELDSVKYGQVFFNQNSMIYNCLKAVKYINEFKSLSKYDTKDVPELSAALRQIPLVS 240

Query: 241 YWVIHTLVASSTELHSYLSGTDGQTQKYLNELAEKFRSLLFTLEKHLQFIREQQEEVELY 300
           YW+IH LVASS ELH YLSG +GQTQKYLNE+ EK  SL+ TLE H+QFIREQQEEVELY
Sbjct: 241 YWIIHILVASSIELHCYLSGVEGQTQKYLNEMTEKVSSLVLTLENHMQFIREQQEEVELY 300

Query: 301 RWLVDQTDHFPTDAALFLAKLIDGKLKPKPLINCSTQLEENIEEFLKEKKLILVVSKRLD 360
           RWLVDQTDHFPTD  LFLAKLI+GK K +PLINCSTQLEE+IEEF+KEKKLIL+VS+ L+
Sbjct: 301 RWLVDQTDHFPTDVTLFLAKLINGKHKARPLINCSTQLEEHIEEFMKEKKLILIVSRSLN 360

Query: 361 VSSEDLEILHLIYNELKKENKYEMVWIPVISDPPNEGDEEAYKHLKSKMKWYVVPFTTKV 420
            S EDLEIL  +Y E+KKENK+EMVWIPVISDPPN+GDEEAY+ LKS+MKW+VVPF  K+
Sbjct: 361 FSREDLEILQSVYKEVKKENKFEMVWIPVISDPPNDGDEEAYEALKSEMKWFVVPFAAKI 420

Query: 421 AGVRFLEEKWQLREDLLMVVLDSKSKIEFTNAIHLTRVWERDAIPLTYERAKALLRRNWI 480
           AGVRFLEE+W+LREDLL+VVLD++SK+EF+NAIHLTRVWE++AIP +Y+R KAL+++NWI
Sbjct: 421 AGVRFLEERWELREDLLLVVLDTQSKVEFSNAIHLTRVWEKEAIPFSYDRVKALMKKNWI 480

Query: 481 ESTVVKFSDQPRLQSW--INQERNIIFYGGHNSKWVRQFEETAEAIKKDPWTREEGITFE 540
           +STV+K++DQPRL+S   INQERN+IFYGGHN +WV+ FE++AEA+K+DP TREEGITFE
Sbjct: 481 DSTVLKYTDQPRLKSLVVINQERNVIFYGGHNPRWVKNFEDSAEAMKRDPVTREEGITFE 540

Query: 541 MIPIGRRDKGEYDPAMMFRFWMAQRSFFIIKHQLHGSNASEDVSRLISYENEHGWAIIAK 600
           ++P+GR +KGE+DPA+MF FWMAQRS+FI+KHQLHGS ASED+S+LISYE E GWAII K
Sbjct: 541 LVPVGRNNKGEHDPAVMFNFWMAQRSYFILKHQLHGSTASEDISQLISYETEKGWAIITK 600

Query: 601 GPTVVFIGGGDLILKAMEEFQTWKKNLRRGGFFSSFKDYFDVLTAKSIHCTHVNILGFSG 660
           GPTVVFIGGGD ILKA++EFQ WKKNLRR GF  SFKDYFD LTA S+HCTHVNI+GFSG
Sbjct: 601 GPTVVFIGGGDSILKAIDEFQIWKKNLRRVGFSGSFKDYFDELTASSLHCTHVNIIGFSG 660

Query: 661 WIPLIIICPVCRRYMGSGIRFTCCHGGPDVL 686
           WIPLII CPVCRRYMGSGIRFTCCHGGPDVL
Sbjct: 661 WIPLIITCPVCRRYMGSGIRFTCCHGGPDVL 690

BLAST of Sgr016489 vs. ExPASy Swiss-Prot
Match: Q9SS87 (Protein SIEVE ELEMENT OCCLUSION B OS=Arabidopsis thaliana OX=3702 GN=SEOB PE=1 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 5.1e-56
Identity = 186/727 (25.58%), Postives = 325/727 (44.70%), Query Frame = 0

Query: 7   KTPTAPLALQTAKEERTLKHFSDDVITHHIYTKHSDDETTKIDLNNYISFIENVIKTADQ 66
           KTP+  +   T      L   SD+ +   +  +    +  ++ +   +S +E+++  A  
Sbjct: 22  KTPSMEMIPATG-----LAMSSDESMMLKLIQQTHSPDAREVQVRGLLSLVEDILDRATL 81

Query: 67  IAETHYGAQGSKGHLVFSDDSLK-YLSSSIDPPICTLHQISKELACKAPGIEGAHKTTLD 126
            +E       S   L   D  ++  + S +D     + +++ E+A K+     +H+ T+ 
Sbjct: 82  DSE---DTNASMLPLPTEDKLMQSSMMSVLDSVSYAIDRVACEIAYKSLTGSDSHEITMS 141

Query: 127 ILSKLTSYPWEAKAALTFTAFATDYGVMWHLEHYSQSDPLAKSLAMIKRVALLKKELGSF 186
           +   L+S+ W+ K  LT  AFA +YG  W L  +   + LAKSLAM+K V +  +     
Sbjct: 142 VFEHLSSFQWDGKLVLTLAAFALNYGEFWLLVQFYSKNQLAKSLAMLKLVPVQNR----V 201

Query: 187 KYRHVFFDPNSLIYSCLQAMKYINELKNL-SKYDTKEVPELSAAVRQIPLVSYWVIHTLV 246
               V    N LI         + EL  L  +Y T +VP+LS  +  IP+  YW I +++
Sbjct: 202 TLESVSQGLNDLIREMKSVTACVVELSELPDRYITPDVPQLSRILSTIPIAVYWTIRSVI 261

Query: 247 ASSTELH--SYLSGTDGQTQKYLNE---LAEKFRSLLFTLEKHLQFIR---EQQEEVELY 306
           A  ++++  + +      TQ  L E   LA K +++   L + L+      E+Q   E  
Sbjct: 262 ACISQINMITAMGHEMMNTQMDLWETSMLANKLKNIHDHLAETLRLCYRHIEKQRSSESL 321

Query: 307 RWLVDQTDHFPTDAALFLAKLIDGKLKPKPLINCSTQLEENIEEFLKEKKLILVVSKRLD 366
           + L    D    D    L  L+  K    PL +  T+ + ++ + L+ K ++L++S  L+
Sbjct: 322 KVLHSLFDTTHIDNMKILTALVHPKPHITPLQDGLTKRKVHL-DVLRRKTVLLLISD-LN 381

Query: 367 VSSEDLEILHLIYNEL---------KKENKYEMVWIPVISDPPNEGD-----EEAYKHLK 426
           +  ++L I   IY E          K    YE+VW+PV+ DP  + +     ++ ++ L+
Sbjct: 382 ILQDELSIFEQIYTESRRNLVGVDGKSHMPYEVVWVPVV-DPIEDFERSPILQKKFEDLR 441

Query: 427 SKMKWYVV--PFTTKVAGVRFLEEKWQLREDLLMVVLDSKSKIEFTNAIHLTRVWERDAI 486
             M WY V  P   +   V F+  +W      ++VV+D +      NA+H+  +W  +A 
Sbjct: 442 DPMPWYSVDSPKLIERHVVEFMRGRWHFMNKPILVVIDPQGNEASLNALHMIWIWGTEAF 501

Query: 487 PLTYERAKALLRRNWIESTVVKFSDQPRLQSWINQERNIIFYGGHNSKWVRQFEETAEAI 546
           P T  R + L RR      ++       + +WI  +  I  YGG +  W+R+F   A+A 
Sbjct: 502 PFTRSREEELWRRETFSLNLIVDGIDSVIFNWIKPDNYIFLYGGDDLDWIRRFTMAAKAT 561

Query: 547 KKDPWTREEGITFEMIPIGRRDKGE--------------------YDPAMMFRFWMAQRS 606
            KD       +  EM  +G+R+                        +PA+M+ FW    S
Sbjct: 562 AKD-----SNVNLEMAYVGKRNHSHREQIRRISEVIRSENLSHSWAEPALMWFFWTRLES 621

Query: 607 FFIIKHQLHGSNASEDV----SRLISYENEHGWAIIAKGPTVVFIGGGDLILKAMEEFQT 666
               K QL  ++  +DV     +++SY+   GWA+++KGP +V I  G +        +T
Sbjct: 622 MLYSKIQLGKADDHDDVMQGIKKILSYDKLGGWALLSKGPEIVMIAHGAIERTMSVYDRT 681

Query: 667 WKKNLRRGGFFSSFKDYF--DVLTAKSIHCTH--VNILGFSGWIPLIIICPVCRRYMGSG 680
           WK ++   G+  +  D+   +VL      C H   +I   SG IP  + C  C+R M   
Sbjct: 682 WKTHVPTKGYTKAMSDHHHDEVLRETGKPCGHFDFHITARSGRIPEKMNCFECQRPMEKY 728

BLAST of Sgr016489 vs. ExPASy Swiss-Prot
Match: Q93XX2 (Protein SIEVE ELEMENT OCCLUSION A OS=Arabidopsis thaliana OX=3702 GN=SEOA PE=1 SV=1)

HSP 1 Score: 161.0 bits (406), Expect = 4.8e-38
Identity = 174/743 (23.42%), Postives = 307/743 (41.32%), Query Frame = 0

Query: 22  RTLKHFSDD-VITHHIYTKHSDDETTKIDLNNYISFIENVIKTADQIAETHYGAQGSKGH 81
           R +   SDD V+   +   HS D     D+ + +S + ++ K+     +    +   K  
Sbjct: 131 RPMFSLSDDRVMADRVLKTHSPD-MIFFDVTSLLSVVNDIFKSHVPSID----SSAPKPS 190

Query: 82  LVFSD----DSLKYLSSSIDPPICTLHQISKELACK-------------APGIEGAHKTT 141
           LVF D     S +  +  ID       QIS E+ CK                ++  + TT
Sbjct: 191 LVFKDYADHTSFETFADLID-------QISCEIDCKCLHGGESHGMMTSGLHLDSRNTTT 250

Query: 142 LDILSKLTSYPWEAKAALTFTAFATDYGVMWHLEHYSQSDPLAKSLAMIKRVALLKKELG 201
             +LS ++ Y W+AK  L  +A A  YGV   L     ++ L KSLA+IK++        
Sbjct: 251 FSVLSLVSKYRWDAKLVLVLSALAVKYGVFLLLAETHATNQLTKSLALIKQLP------- 310

Query: 202 SFKYRHVFFDPNSLIYSCLQAMKYINELKNLSK--YDTKEVPE---LSAAVRQIPLVSYW 261
                 +F   N+L     +    + ++ +L+    D  ++P     +A    IP   YW
Sbjct: 311 -----SIFSRQNALHQRLDKTRILMQDMVDLTTTIIDIYQLPPNHITAAFTDHIPTAVYW 370

Query: 262 VIHTLVASSTELHSYLSGTDGQTQKYLNE--------------------LAEKFRSLLFT 321
           ++  ++       S++SG  G  Q  +                      L E+F+    T
Sbjct: 371 IVRCVLICV----SHISGASGFKQDQIMSFMEVSEIHENSERLRKINAYLLEQFKKSKMT 430

Query: 322 LEKHLQFIREQQEEVELYRWLVDQTDHFPTDAALFLAKLID------GKLKPKPLINCST 381
           +E+ +    E QE ++ +  ++   D  P    L L + ID      G  K +  IN  T
Sbjct: 431 IEEGI-IEEEYQELIQTFTTII-HVDVVP--PLLRLLRPIDFLYHGAGVSKRRVGINVLT 490

Query: 382 QLEENIEEFLKEKKLILVVSKRLDVSSEDLEILHLIYNELKKENKYEMVWIPVISDPPNE 441
           Q            K +L++   L+   ++L IL  +Y E  +++ +E++W+PV  D   E
Sbjct: 491 Q------------KHVLLLISDLENIEKELYILESLYTEAWQQS-FEILWVPV-QDFWTE 550

Query: 442 GDEEAYKHLKSKMKWYVV--PFTTKVAGVRFLEEKWQLREDLLMVVLDSKSKIEFTNAIH 501
            D+  ++ L   M+WYV+  P   + A +RF+ E W  +   ++V LD K ++  TNA  
Sbjct: 551 ADDAKFEALHMNMRWYVLGEPRKLRRAAIRFVREWWGFKNRPILVALDPKGQVMSTNAFP 610

Query: 502 LTRVWERDAIPLTYERAKAL-LRRNWIESTVVKFSDQPRLQSWINQERNIIFYGGHNSKW 561
           +  +W+  A P T  R + L   + W    ++  +D   L   ++  + I  YGG + +W
Sbjct: 611 MVWIWQPFAHPFTTARERDLWSEQEWNLEFLIDGTDPHSLNQLVD-GKYICLYGGEDMQW 670

Query: 562 VRQFEETAEAIKKDPWTREEGITFEMIPIGRRD-KGEYDPAM-----------------M 621
           ++ F      + K        I  EM+ +G+R+ K    P +                 +
Sbjct: 671 IKNFTSLWRNVAK-----AANIQLEMVYVGKRNPKNGIQPIINTIREENLSHTLPDLFQI 730

Query: 622 FRFWMAQRSFFIIKHQL---HGSNASE------------DVSRLISYENE-HGWAIIAKG 679
           + FW    S +  K ++   HG    E            +V  ++ Y  E  GW +++K 
Sbjct: 731 WFFWTRVESMWESKQRMLKAHGIKGREGFKEEEKDLVLQEVVAMLGYGGEGDGWGLVSKA 790

BLAST of Sgr016489 vs. ExPASy Swiss-Prot
Match: Q9FXE2 (Protein SIEVE ELEMENT OCCLUSION C OS=Arabidopsis thaliana OX=3702 GN=SEOC PE=4 SV=2)

HSP 1 Score: 104.0 bits (258), Expect = 6.9e-21
Identity = 120/604 (19.87%), Postives = 235/604 (38.91%), Query Frame = 0

Query: 102 LHQISKELACKAPGIEGAHKTTLDILSKLTSYPWEAKAALTFTAFATDYGVMWHLEHYSQ 161
           + +IS ++ C   G     K T+ +   L  Y W+AKA L     A  YG +    H + 
Sbjct: 77  IFRISVQMLCPCTGENEIRKRTMVLFDLLKEYRWDAKAVLVLGVLAATYGGLLLPVHLAI 136

Query: 162 SDPLAKSLAMIKRVALLKKELGSFKYRHVFFDPNSLIYSCLQAMKYINELKNLSKYDTK- 221
            DP+A S+A + ++ + +      K+R      N LI + +   K I + + +     K 
Sbjct: 137 CDPVAASIAKLNQLPIERT-----KFRPWLESLNLLIKAMVDVTKCIIKFEKIPFKQAKL 196

Query: 222 EVPELSAAVRQIPLVSYWVIHTLVA---------------SSTELHSYLSGTDGQTQKYL 281
           +   L   +  I L +Y V+ + +                 S +  + LS    +    L
Sbjct: 197 DNNILGETLSNIYLTTYRVVKSALTCMQQIPYFKQTQQAKKSRKTAAELSIESRRAAGEL 256

Query: 282 NELAEKFRSLLFTLEKHLQFIREQ-QEEVELYRWLVDQTDHFPTDAALFLAKLIDGKLKP 341
           + L  +  ++   L K ++    Q +EE+      ++   H      L L   +   L  
Sbjct: 257 SSLGYQLLNIHTRLNKQVEDCSTQIEEEINQRLRNINIETHQDNQDVLHLLFSLQDDL-- 316

Query: 342 KPLINCSTQLEENIEEFLKEKKLILVVSKRLDVSSEDLEILHLIYNELKKENKYEMVWIP 401
            PL   S Q+  +I E   +  L+L+    ++     L+ L+   +    E  YE++W+P
Sbjct: 317 -PLQQYSRQI--SITEVQDKVTLLLLSKPPVEPLFFLLQQLYDHPSNTNTEQNYEIIWVP 376

Query: 402 VISDPP-NEGDEEAYKHLKSKMKWYVV--PFTTKVAGVRFLEEKWQLRE-DLLMVVLDSK 461
           + S     + ++E +    + + W  V  P+      + F +++W  ++ + ++VV+DS 
Sbjct: 377 IPSSQKWTDEEKEIFDFYSNSLPWISVRQPWLMSSTILNFFKQEWHYKDNEAMLVVIDSN 436

Query: 462 SKIEFTNAIHLTRVWERDAIPLTYERAKALLRRNWIESTVVKFSDQPRLQSWINQERNII 521
            +    NA+ +  +W   A P +  R   L + +     ++     P  +      R I 
Sbjct: 437 GRFVNMNAMDMVLIWGVKAYPFSVSREDELWKEHGWSINLLLDGIHPTFEG-----REIC 496

Query: 522 FYGGHNSKWVRQFEETAEAIKKDPWTREEGITFEMIPIGRRDKGE---------YDPAMM 581
            +G  N  W+ +F   A  I      +  G   E+I +  + + E         + P + 
Sbjct: 497 IFGSENLDWIDEFVSLARKI------QNLGFQLELIYLSNQRRDERAMEESSILFSPTLQ 556

Query: 582 FRFWMAQRSF------FIIKHQLHGSNASEDVSRLI--SYENEHGWAIIAKGPTVVFIGG 641
             FW+   S        I+          E+V  L+   Y    GW II  G T   +  
Sbjct: 557 QLFWLRLESIERSKLKRIVIEPSKPDRVFEEVRNLLDFDYGKHRGWGIIGNGSTAETV-D 616

Query: 642 GDLILKAMEEFQTWKKNLRRGGFFSSFKDYFDVLTAKSIHCTHVNILGFSGWIPL-IIIC 667
           G+ + + M +   W +  +  G    F +  ++   K    +H  ++ F   + + ++ C
Sbjct: 617 GEKMTERMRKIVRWGEYAKGLG----FTEAIEIAAEKPCELSHTAVVPFEEALTMKVVTC 654

BLAST of Sgr016489 vs. ExPASy TrEMBL
Match: A0A6J1C9F2 (protein SIEVE ELEMENT OCCLUSION B-like OS=Momordica charantia OX=3673 GN=LOC111009603 PE=4 SV=1)

HSP 1 Score: 1175.2 bits (3039), Expect = 0.0e+00
Identity = 579/686 (84.40%), Postives = 624/686 (90.96%), Query Frame = 0

Query: 1   MSAPPPKTPTAPLALQTAKEERTLKHFSDDVITHHIYTKHSDDETTKIDLNNYISFIENV 60
           MS P PKTP APLAL  A EER+LK+FSDDVIT HIYTKHSDDE  KIDLNNYISFIENV
Sbjct: 1   MSTPRPKTPAAPLAL-AANEERSLKYFSDDVITSHIYTKHSDDEAMKIDLNNYISFIENV 60

Query: 61  IKTADQIAETHYG-AQGSKGHLVFSDDSLKYLSSSIDPPICTLHQISKELACKAPGIEGA 120
           +KTADQIAETH G  QGSKGH VFSDD LKY  SSIDPP+CTLHQISKELACKAPGIE A
Sbjct: 61  VKTADQIAETHLGRVQGSKGHFVFSDDPLKY-PSSIDPPLCTLHQISKELACKAPGIETA 120

Query: 121 HKTTLDILSKLTSYPWEAKAALTFTAFATDYGVMWHLEHYSQSDPLAKSLAMIKRVALLK 180
           HKTTLDIL+KL SYPWEAKAALTF AFATDYGV+WHL+HYS SDPLAKSLAMIKRVA LK
Sbjct: 121 HKTTLDILNKLISYPWEAKAALTFAAFATDYGVIWHLDHYSHSDPLAKSLAMIKRVAWLK 180

Query: 181 KELGSFKYRHVFFDPNSLIYSCLQAMKYINELKNLSKYDTKEVPELSAAVRQIPLVSYWV 240
           KEL SFKYRHV    NSLIYSCLQA+K INELKN+SKYDTKEVPELSAA+RQIPLVSYW+
Sbjct: 181 KELESFKYRHV----NSLIYSCLQAIKNINELKNISKYDTKEVPELSAALRQIPLVSYWI 240

Query: 241 IHTLVASSTELHSYLSGTDGQTQKYLNELAEKFRSLLFTLEKHLQFIREQQEEVELYRWL 300
           IHTLVASSTELHSYLSG +GQT KYLNEL EKFRSLLFT EK LQFIREQQEEVELY+ L
Sbjct: 241 IHTLVASSTELHSYLSGAEGQTLKYLNELDEKFRSLLFTFEKQLQFIREQQEEVELYKSL 300

Query: 301 VDQTDHFPTDAALFLAKLIDGKLKPKPLINCSTQLEENIEEFLKEKKLILVVSKRLDVSS 360
           V QTDHFPTD  LFL+KLIDGK KP+PLINCSTQLEE+IEEFLKEK LILVVSK L  S 
Sbjct: 301 VGQTDHFPTDVTLFLSKLIDGKHKPQPLINCSTQLEEHIEEFLKEKNLILVVSKGLKFSM 360

Query: 361 EDLEILHLIYNELKKENKYEMVWIPVISDPPNEGDEEAYKHLKSKMKWYVVPFTTKVAGV 420
           EDLEILH+IY+ELK+ENKYE+VW+PVISDPPNEGDEEAY+ LKSKMKWY +PFTTKVAGV
Sbjct: 361 EDLEILHVIYDELKRENKYEIVWVPVISDPPNEGDEEAYESLKSKMKWYALPFTTKVAGV 420

Query: 421 RFLEEKWQLREDLLMVVLDSKSKIEFTNAIHLTRVWERDAIPLTYERAKALLRRNWIEST 480
           RFLEEKWQLREDL++VVLDSKSKIEFTNA+HLTRVW+R+AIP TYERAKALLR+NWI+ST
Sbjct: 421 RFLEEKWQLREDLMVVVLDSKSKIEFTNAVHLTRVWKREAIPFTYERAKALLRKNWIDST 480

Query: 481 VVKFSDQPRLQSWINQERNIIFYGGHNSKWVRQFEETAEAIKKDPWTREEGITFEMIPIG 540
           VVKFSDQPRL+SWINQERNIIFYGGHN  WVR+FEETAEAIK+DP  REEG TFEM+PIG
Sbjct: 481 VVKFSDQPRLRSWINQERNIIFYGGHNPNWVRKFEETAEAIKRDPLAREEGATFEMVPIG 540

Query: 541 RRDKGEYDPAMMFRFWMAQRSFFIIKHQLHGSNASEDVSRLISYENEHGWAIIAKGPTVV 600
           R DKGE+DPA+MFRFWM QRSFF IKHQLHGS+ASEDVS+LISYENE+GWAII KGPTV+
Sbjct: 541 RNDKGEHDPAVMFRFWMVQRSFFNIKHQLHGSSASEDVSQLISYENENGWAIIVKGPTVI 600

Query: 601 FIGGGDLILKAMEEFQTWKKNLRRGGFFSSFKDYFDVLTAKSIHCTHVNILGFSGWIPLI 660
           F+GGGDLILKAMEEFQ WKKNLR  GF +SFKDY+  LTAKS HCTHVNILGFSGWIPLI
Sbjct: 601 FVGGGDLILKAMEEFQAWKKNLRWEGFSASFKDYYHELTAKSPHCTHVNILGFSGWIPLI 660

Query: 661 IICPVCRRYMGSGIRFTCCHGGPDVL 686
           I+CP+C RYMGSGIRFTCCHGGPDVL
Sbjct: 661 IVCPMCHRYMGSGIRFTCCHGGPDVL 680

BLAST of Sgr016489 vs. ExPASy TrEMBL
Match: A0A6J1H3Z4 (protein SIEVE ELEMENT OCCLUSION B-like OS=Cucurbita moschata OX=3662 GN=LOC111459939 PE=4 SV=1)

HSP 1 Score: 1066.2 bits (2756), Expect = 5.6e-308
Identity = 510/691 (73.81%), Postives = 599/691 (86.69%), Query Frame = 0

Query: 1   MSAPPPKTPTAPLAL----QTAKEERTLKHFSDDVITHHIYTKHSDDETTKIDLNNYISF 60
           MS   PK+  A LAL    QT KEE  LKH SDDVIT+ I+   +D+E TKIDL+NY+ F
Sbjct: 1   MSLLRPKSSAAALALALSQQTDKEEPGLKHLSDDVITNRIFDFTTDEEMTKIDLDNYVLF 60

Query: 61  IENVIKTADQIAETHYGAQGSKGHLVFSDDSLKYLSSSIDPPICTLHQISKELACKAPGI 120
           IENV+K +DQI    + AQGSKGH V ++D++ Y  S IDPPICTLHQIS E+ACK PG+
Sbjct: 61  IENVLKASDQICVASHWAQGSKGHFVLAEDTMNY-PSPIDPPICTLHQISSEMACKPPGV 120

Query: 121 EGAHKTTLDILSKLTSYPWEAKAALTFTAFATDYGVMWHLEHYSQSDPLAKSLAMIKRVA 180
           E  HKTTLDILSKLT YPWEAKA L FTAF T+YGV+WHL+++S SDPLAKSLAMIKRV 
Sbjct: 121 ETVHKTTLDILSKLTRYPWEAKAVLIFTAFVTNYGVLWHLDNHSHSDPLAKSLAMIKRVG 180

Query: 181 LLKKELGSFKYRHVFFDPNSLIYSCLQAMKYINELKNLSKYDTKEVPELSAAVRQIPLVS 240
           LL+KEL S KY  VFF+ NS+IY+CL+A+KYINE K+LSKYDTK+VPELSAA+RQIPLVS
Sbjct: 181 LLRKELESVKYGQVFFNQNSMIYNCLKAVKYINEFKSLSKYDTKDVPELSAALRQIPLVS 240

Query: 241 YWVIHTLVASSTELHSYLSGTDGQTQKYLNELAEKFRSLLFTLEKHLQFIREQQEEVELY 300
           YW+IH LVASS ELH YLSG +GQTQKYLNE+ EK  SL+ TLE H+QFIREQQEEVELY
Sbjct: 241 YWIIHILVASSIELHCYLSGVEGQTQKYLNEMTEKVSSLVLTLENHMQFIREQQEEVELY 300

Query: 301 RWLVDQTDHFPTDAALFLAKLIDGKLKPKPLINCSTQLEENIEEFLKEKKLILVVSKRLD 360
           RWLVDQTDHFPTD  LFLAKLI+GK K +PLINCSTQLEE+IEEF+KEKKLIL+VS+ L+
Sbjct: 301 RWLVDQTDHFPTDVTLFLAKLINGKHKARPLINCSTQLEEHIEEFMKEKKLILIVSRSLN 360

Query: 361 VSSEDLEILHLIYNELKKENKYEMVWIPVISDPPNEGDEEAYKHLKSKMKWYVVPFTTKV 420
            S EDLEIL  +Y E+KKENK+EMVWIPVISDPPN+GDEEAY+ LKS+MKW+VVPF  K+
Sbjct: 361 FSREDLEILQSVYKEVKKENKFEMVWIPVISDPPNDGDEEAYEALKSEMKWFVVPFAAKI 420

Query: 421 AGVRFLEEKWQLREDLLMVVLDSKSKIEFTNAIHLTRVWERDAIPLTYERAKALLRRNWI 480
           AGVRFLEE+W+LREDLL+VVLD++SK+EF+NAIHLTRVWE++AIP +Y+R KAL+++NWI
Sbjct: 421 AGVRFLEERWELREDLLLVVLDTQSKVEFSNAIHLTRVWEKEAIPFSYDRVKALMKKNWI 480

Query: 481 ESTVVKFSDQPRLQSW--INQERNIIFYGGHNSKWVRQFEETAEAIKKDPWTREEGITFE 540
           +STV+K++DQPRL+S   INQERN+IFYGGHN +WV+ FE++AEA+K+DP TREEGITFE
Sbjct: 481 DSTVLKYTDQPRLKSLVVINQERNVIFYGGHNPRWVKNFEDSAEAMKRDPVTREEGITFE 540

Query: 541 MIPIGRRDKGEYDPAMMFRFWMAQRSFFIIKHQLHGSNASEDVSRLISYENEHGWAIIAK 600
           ++P+GR +KGE+DPA+MF FWMAQRS+FI+KHQLHGS ASED+SRLISYE E GWAII K
Sbjct: 541 LVPVGRNNKGEHDPAVMFNFWMAQRSYFILKHQLHGSTASEDISRLISYETEKGWAIITK 600

Query: 601 GPTVVFIGGGDLILKAMEEFQTWKKNLRRGGFFSSFKDYFDVLTAKSIHCTHVNILGFSG 660
           GP VVFIGGGDLILKA++EFQ WKKNLRR GF  SFKDYFD LTA S+HCTHVNI+GFSG
Sbjct: 601 GPIVVFIGGGDLILKAIDEFQVWKKNLRRVGFSGSFKDYFDELTASSLHCTHVNIIGFSG 660

Query: 661 WIPLIIICPVCRRYMGSGIRFTCCHGGPDVL 686
           WIPLII CPVCRRYMGSGIRFTCCHGGPDVL
Sbjct: 661 WIPLIITCPVCRRYMGSGIRFTCCHGGPDVL 690

BLAST of Sgr016489 vs. ExPASy TrEMBL
Match: A0A6J1KWJ8 (protein SIEVE ELEMENT OCCLUSION B-like OS=Cucurbita maxima OX=3661 GN=LOC111499359 PE=4 SV=1)

HSP 1 Score: 1060.4 bits (2741), Expect = 3.1e-306
Identity = 501/675 (74.22%), Postives = 592/675 (87.70%), Query Frame = 0

Query: 13  LALQTAKEERTLKHFSDDVITHHIYTKHSDDETTKIDLNNYISFIENVIKTADQIAETHY 72
           L+ QT KEE  LKH SDDVIT+ I+   +D+E  KIDL+NY+ FIENV+K +DQI    +
Sbjct: 17  LSQQTDKEEPDLKHLSDDVITNRIFNFTTDEEMMKIDLDNYVVFIENVLKASDQICVASH 76

Query: 73  GAQGSKGHLVFSDDSLKYLSSSIDPPICTLHQISKELACKAPGIEGAHKTTLDILSKLTS 132
            AQGSKG+ V ++D++ Y SS IDPPICTLHQIS E+ACK PG+E  HKTTLDILSKLT 
Sbjct: 77  WAQGSKGNFVLAEDTMNYPSSPIDPPICTLHQISSEMACKPPGVETVHKTTLDILSKLTR 136

Query: 133 YPWEAKAALTFTAFATDYGVMWHLEHYSQSDPLAKSLAMIKRVALLKKELGSFKYRHVFF 192
           YPWEAKA L F AF T+YGV+WHL+++S SDPLAKSLAMIKRV LL+KEL S KY  VFF
Sbjct: 137 YPWEAKAMLIFAAFMTNYGVLWHLDNHSHSDPLAKSLAMIKRVGLLRKELESVKYGQVFF 196

Query: 193 DPNSLIYSCLQAMKYINELKNLSKYDTKEVPELSAAVRQIPLVSYWVIHTLVASSTELHS 252
           + NS+IY+CL+A+KYI E K+LSKYDTK+VPELSAA+R+IPLVSYW+IH LVASS ELH 
Sbjct: 197 NQNSMIYNCLKAVKYIIEFKSLSKYDTKDVPELSAALRRIPLVSYWIIHILVASSIELHC 256

Query: 253 YLSGTDGQTQKYLNELAEKFRSLLFTLEKHLQFIREQQEEVELYRWLVDQTDHFPTDAAL 312
           YLSG +GQTQKYLNE+ EK  +L+ TLE H+QFIREQQEEVELYRWLVDQTDHFPTD  L
Sbjct: 257 YLSGVEGQTQKYLNEMTEKVSTLVLTLENHMQFIREQQEEVELYRWLVDQTDHFPTDVTL 316

Query: 313 FLAKLIDGKLKPKPLINCSTQLEENIEEFLKEKKLILVVSKRLDVSSEDLEILHLIYNEL 372
           FLAKLI+GK K +PLINCSTQLEE+IEEF+KEKKLIL+VS+ L+ S EDLEIL  IY E+
Sbjct: 317 FLAKLINGKHKARPLINCSTQLEEHIEEFMKEKKLILIVSRSLNFSREDLEILQSIYKEV 376

Query: 373 KKENKYEMVWIPVISDPPNEGDEEAYKHLKSKMKWYVVPFTTKVAGVRFLEEKWQLREDL 432
           KKENK+EMVWIPVISDPPN+GDEEAY+ LKS+MKW+VVPF TK+AGVRFLEE+W+LREDL
Sbjct: 377 KKENKFEMVWIPVISDPPNDGDEEAYEALKSEMKWFVVPFATKIAGVRFLEERWELREDL 436

Query: 433 LMVVLDSKSKIEFTNAIHLTRVWERDAIPLTYERAKALLRRNWIESTVVKFSDQPRLQSW 492
           L+VVLD++SK+EF+NAIHLTRVWE++AIPL+Y+R K L+++NWI+STV+KF+DQPRL+S 
Sbjct: 437 LLVVLDTQSKVEFSNAIHLTRVWEKEAIPLSYDRVKVLMKKNWIDSTVLKFTDQPRLKSL 496

Query: 493 --INQERNIIFYGGHNSKWVRQFEETAEAIKKDPWTREEGITFEMIPIGRRDKGEYDPAM 552
             INQERN+IFYGGHN +WV+ FEE+AEA+K+DP TREEGITFE++P+GR +KGE+DPA+
Sbjct: 497 VVINQERNVIFYGGHNPRWVKNFEESAEAMKRDPVTREEGITFELVPVGRNNKGEHDPAV 556

Query: 553 MFRFWMAQRSFFIIKHQLHGSNASEDVSRLISYENEHGWAIIAKGPTVVFIGGGDLILKA 612
           MF FWMAQRS+FI+KHQLHGS ASED+SR+ISYE E GWAII KGPTVVFIGGGDLILKA
Sbjct: 557 MFNFWMAQRSYFILKHQLHGSTASEDISRMISYETEKGWAIITKGPTVVFIGGGDLILKA 616

Query: 613 MEEFQTWKKNLRRGGFFSSFKDYFDVLTAKSIHCTHVNILGFSGWIPLIIICPVCRRYMG 672
           ++EFQ WKKNLR+ GF  SFKDYFD LTA S+HCTHVNI+GFSGWIPLII CPVCRRYMG
Sbjct: 617 IDEFQIWKKNLRQVGFSGSFKDYFDELTASSLHCTHVNIIGFSGWIPLIITCPVCRRYMG 676

Query: 673 SGIRFTCCHGGPDVL 686
           SGIRFTCCHGGPDVL
Sbjct: 677 SGIRFTCCHGGPDVL 691

BLAST of Sgr016489 vs. ExPASy TrEMBL
Match: A0A6J1EQ44 (protein SIEVE ELEMENT OCCLUSION B-like OS=Cucurbita moschata OX=3662 GN=LOC111436801 PE=4 SV=1)

HSP 1 Score: 1031.6 bits (2666), Expect = 1.5e-297
Identity = 497/689 (72.13%), Postives = 587/689 (85.20%), Query Frame = 0

Query: 3   APPPKTPTAPLAL----QTAKEERTLKHFSDDVITHHIYTKHSDDETTKIDLNNYISFIE 62
           AP PK    PLAL    +T KE+  LKH SDDVI +H++TKH DDET KIDLN+YISFIE
Sbjct: 6   APRPKISVVPLALALMQKTDKEQTNLKHLSDDVIMNHLFTKHGDDETIKIDLNDYISFIE 65

Query: 63  NVIKTADQIAETHYGAQGSKGHLVFSDDSLKYLSSSIDPPICTLHQISKELACKAPGIEG 122
           NVIK++DQI+   + AQGSK H+  SDDS  YL SSI+PP+CTLH ISK++ CKA GI+ 
Sbjct: 66  NVIKSSDQISAASHWAQGSKVHVELSDDSFTYL-SSIEPPVCTLHDISKQMGCKAAGIDI 125

Query: 123 AHKTTLDILSKLTSYPWEAKAALTFTAFATDYGVMWHLEHYSQSDPLAKSLAMIKRVALL 182
           AHKTTLDILSKLT Y WEAKA L F AFA +YG +WHL++YS+SDPLAKSLAMIKRV  L
Sbjct: 126 AHKTTLDILSKLTKYSWEAKAVLIFAAFAMNYGALWHLDNYSRSDPLAKSLAMIKRVGSL 185

Query: 183 KKELGSFKYRHVFFDPNSLIYSCLQAMKYINELKNLSKYDTKEVPELSAAVRQIPLVSYW 242
           +KEL S +Y  VFF PNSLIYSCL+A+KY+NE KNLSKYD KEVPELSAA+R+IPLVSYW
Sbjct: 186 RKELDSVQYGQVFFGPNSLIYSCLKAIKYMNEFKNLSKYDIKEVPELSAALRRIPLVSYW 245

Query: 243 VIHTLVASSTELHSYLSGTDGQTQKYLNELAEKFRSLLFTLEKHLQFIREQQEEVELYRW 302
           ++H LVASS +LH YLSGT+GQT KYLNEL+EK RS+L TLEKH QFI++QQEEVELYRW
Sbjct: 246 IVHALVASSIDLHCYLSGTEGQTHKYLNELSEKKRSILVTLEKHHQFIQKQQEEVELYRW 305

Query: 303 LVDQTDHFPTDAALFLAKLIDGKLKPKPLINCSTQLEENIEEFLKEKKLILVVSKRLDVS 362
           LVDQTDHFP D  LFL+KLI GK K +PLINCST+LEE IE+FLKEK LIL+VSKRLDVS
Sbjct: 306 LVDQTDHFPIDITLFLSKLIHGKHKARPLINCSTRLEEYIEDFLKEKNLILIVSKRLDVS 365

Query: 363 SEDLEILHLIYNELKKENKYEMVWIPVISDPPNEGDEEAYKHLKSKMKWYVVPFTTKVAG 422
            EDL+ L+++YNE+KK NK+E+VW+PVISDPP EGDEEAY+ L SKMKWY VPF TKVAG
Sbjct: 366 KEDLDSLNVVYNEVKKGNKHEIVWVPVISDPPAEGDEEAYEALISKMKWYAVPFDTKVAG 425

Query: 423 VRFLEEKWQLREDLLMVVLDSKSKIEFTNAIHLTRVWERDAIPLTYERAKALLRRNWIES 482
           +RFLEEKW++REDLL+VVL+++SKIEF NA+HLTR+WE++AIP TYERA ALL+R+WI+S
Sbjct: 426 LRFLEEKWEVREDLLIVVLNTQSKIEFPNAVHLTRIWEKEAIPFTYERANALLKRSWIDS 485

Query: 483 TVVKFSDQPRLQS--WINQERNIIFYGGHNSKWVRQFEETAEAIKKDPWTREEGITFEMI 542
           TVVKF+DQPRL S   IN+ER +IFYGGHN  W+++FE+TAEAIK+D   REEGITFE++
Sbjct: 486 TVVKFTDQPRLNSLAMINKERTMIFYGGHNQGWIKRFEDTAEAIKRDLMLREEGITFELV 545

Query: 543 PIGRRDKGEYDPAMMFRFWMAQRSFFIIKHQLHGSNASEDVSRLISYENEHGWAIIAKGP 602
           P+G   KGE DP +M RFW AQRSFFI+KHQL GS A+ED+SRLISYENE GWAI+ KG 
Sbjct: 546 PLGLNQKGEPDPTIMSRFWTAQRSFFILKHQLQGSTATEDISRLISYENEKGWAIVTKGQ 605

Query: 603 TVVFIGGGDLILKAMEEFQTWKKNLRRGGFFSSFKDYFDVLTAKSIHCTHVNILGFSGWI 662
           TVV +GGGDLI+KA+EEFQTWKKNLRR GF  SFKDYFD LT+KS+ CTHVNI+G+SGWI
Sbjct: 606 TVVMVGGGDLIVKALEEFQTWKKNLRRVGFSGSFKDYFDELTSKSLECTHVNIMGYSGWI 665

Query: 663 PLIIICPVCRRYMGSGIRFTCCHGGPDVL 686
           PL ++CPVCRRYMGSGIRFTCCHG P+VL
Sbjct: 666 PLTVMCPVCRRYMGSGIRFTCCHGRPNVL 693

BLAST of Sgr016489 vs. ExPASy TrEMBL
Match: A0A5A7UTQ8 (Protein SIEVE ELEMENT OCCLUSION B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1503G00130 PE=4 SV=1)

HSP 1 Score: 1025.0 bits (2649), Expect = 1.4e-295
Identity = 492/692 (71.10%), Postives = 590/692 (85.26%), Query Frame = 0

Query: 1   MSAPPPKTPTAPLALQ-----TAKEERTLKHFSDDVITHHIYTKHSDDETTKIDLNNYIS 60
           MS   PKT    LAL        +EE++LKH SDD IT+ I+T +SD ET KID+++YI 
Sbjct: 1   MSIFRPKTSATALALALPQEIDKEEEKSLKHLSDDFITNRIFTINSDAETMKIDIDSYIL 60

Query: 61  FIENVIKTADQIAETHYGAQGSKGHLVFSDDSLKYLSSSIDPPICTLHQISKELACKAPG 120
           FIE+VIK++D+IA   + A+GSKGH   +D++LKY  + IDPPICTLHQIS E+ CK PG
Sbjct: 61  FIESVIKSSDKIAVASHWAKGSKGHFELADETLKY-PTQIDPPICTLHQISTEMICKDPG 120

Query: 121 IEGAHKTTLDILSKLTSYPWEAKAALTFTAFATDYGVMWHLEHYSQSDPLAKSLAMIKRV 180
            E AH+TTLDIL KL  Y W+AKA L FTAFAT YGV+WHL++YS SD LAKSLA IKRV
Sbjct: 121 AETAHQTTLDILQKLAKYSWDAKAVLIFTAFATTYGVLWHLDNYSHSDTLAKSLATIKRV 180

Query: 181 ALLKKELGSFKYRHVFFDPNSLIYSCLQAMKYINELKNLSKYDTKEVPELSAAVRQIPLV 240
           ALL+KEL S KY  VFF+PNS+IY+C++A+KYINE +NLSKYDTK+VPELSAA+RQIPLV
Sbjct: 181 ALLRKELDSVKYGQVFFNPNSMIYNCMKAIKYINEFRNLSKYDTKDVPELSAALRQIPLV 240

Query: 241 SYWVIHTLVASSTELHSYLSGTDGQTQKYLNELAEKFRSLLFTLEKHLQFIREQQEEVEL 300
           SYW+IHTLVASS ELH YLSG  GQT KYLNEL+EK  S+L TLE HLQ IREQ EEVEL
Sbjct: 241 SYWIIHTLVASSIELHCYLSGVQGQTHKYLNELSEKSESVLLTLENHLQLIREQIEEVEL 300

Query: 301 YRWLVDQTDHFPTDAALFLAKLIDGKLKPKPLINCSTQLEENIEEFLKEKKLILVVSKRL 360
           YRWLVDQTDHFPTD  LFL+KLIDGK K +PLINCSTQLEE IE+FLKEKKLIL+VSKRL
Sbjct: 301 YRWLVDQTDHFPTDITLFLSKLIDGKHKARPLINCSTQLEEYIEDFLKEKKLILIVSKRL 360

Query: 361 DVSSEDLEILHLIYNELKKENKYEMVWIPVISDPPNEGDEEAYKHLKSKMKWYVVPFTTK 420
           D+S+EDLEIL+ IYNE+KKENK+EMVWIPVI DPP +GDEE Y++LKS MKWY+VPF TK
Sbjct: 361 DISTEDLEILYSIYNEVKKENKFEMVWIPVIPDPPMDGDEEGYEYLKSVMKWYMVPFNTK 420

Query: 421 VAGVRFLEEKWQLREDLLMVVLDSKSKIEFTNAIHLTRVWERDAIPLTYERAKALLRRNW 480
           +AG+RFLEE+W+LRED+LMVVL+++SK+EF+N IHL R+WE++A+P TY+RAKALL++NW
Sbjct: 421 IAGMRFLEERWELREDILMVVLNTQSKVEFSNGIHLARIWEKEALPFTYDRAKALLKKNW 480

Query: 481 IESTVVKFSDQPRLQSW--INQERNIIFYGGHNSKWVRQFEETAEAIKKDPWTREEGITF 540
           I+STVVKF+DQPRL+S   INQERN+IFYGGHN +W+++FEE+AE +K+DP TREEGITF
Sbjct: 481 IDSTVVKFTDQPRLRSLVVINQERNVIFYGGHNLRWIKKFEESAEVMKRDPTTREEGITF 540

Query: 541 EMIPIGRRDKGEYDPAMMFRFWMAQRSFFIIKHQLHGSNASEDVSRLISYENEHGWAIIA 600
           E+ P+G  +KGE DPA+MFRFWMAQRS+FI+KHQL GS A+ED+SRLISYE E GWAII 
Sbjct: 541 ELAPVG-LNKGEQDPALMFRFWMAQRSYFILKHQLQGSTATEDISRLISYETEDGWAIIT 600

Query: 601 KGPTVVFIGGGDLILKAMEEFQTWKKNLRRGGFFSSFKDYFDVLTAKSIHCTHVNILGFS 660
           KGPTVVF+ GGDLILKAM+EF  WKKN+RR GF  SFK++FD LTA S+HCT+VN++GFS
Sbjct: 601 KGPTVVFVAGGDLILKAMDEFHLWKKNMRRLGFSGSFKEHFDELTATSLHCTNVNLIGFS 660

Query: 661 GWIPLIIICPVCRRYMGSGIRFTCCHGGPDVL 686
           GWIPL + CP+CRRYMGSGIRFTCCHGGPDVL
Sbjct: 661 GWIPLFLTCPMCRRYMGSGIRFTCCHGGPDVL 690

BLAST of Sgr016489 vs. TAIR 10
Match: AT3G01680.1 (CONTAINS InterPro DOMAIN/s: Mediator complex subunit Med28 (InterPro:IPR021640); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G01670.1); Has 122 Blast hits to 112 proteins in 13 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 122; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 220.7 bits (561), Expect = 3.6e-57
Identity = 186/727 (25.58%), Postives = 325/727 (44.70%), Query Frame = 0

Query: 7   KTPTAPLALQTAKEERTLKHFSDDVITHHIYTKHSDDETTKIDLNNYISFIENVIKTADQ 66
           KTP+  +   T      L   SD+ +   +  +    +  ++ +   +S +E+++  A  
Sbjct: 22  KTPSMEMIPATG-----LAMSSDESMMLKLIQQTHSPDAREVQVRGLLSLVEDILDRATL 81

Query: 67  IAETHYGAQGSKGHLVFSDDSLK-YLSSSIDPPICTLHQISKELACKAPGIEGAHKTTLD 126
            +E       S   L   D  ++  + S +D     + +++ E+A K+     +H+ T+ 
Sbjct: 82  DSE---DTNASMLPLPTEDKLMQSSMMSVLDSVSYAIDRVACEIAYKSLTGSDSHEITMS 141

Query: 127 ILSKLTSYPWEAKAALTFTAFATDYGVMWHLEHYSQSDPLAKSLAMIKRVALLKKELGSF 186
           +   L+S+ W+ K  LT  AFA +YG  W L  +   + LAKSLAM+K V +  +     
Sbjct: 142 VFEHLSSFQWDGKLVLTLAAFALNYGEFWLLVQFYSKNQLAKSLAMLKLVPVQNR----V 201

Query: 187 KYRHVFFDPNSLIYSCLQAMKYINELKNL-SKYDTKEVPELSAAVRQIPLVSYWVIHTLV 246
               V    N LI         + EL  L  +Y T +VP+LS  +  IP+  YW I +++
Sbjct: 202 TLESVSQGLNDLIREMKSVTACVVELSELPDRYITPDVPQLSRILSTIPIAVYWTIRSVI 261

Query: 247 ASSTELH--SYLSGTDGQTQKYLNE---LAEKFRSLLFTLEKHLQFIR---EQQEEVELY 306
           A  ++++  + +      TQ  L E   LA K +++   L + L+      E+Q   E  
Sbjct: 262 ACISQINMITAMGHEMMNTQMDLWETSMLANKLKNIHDHLAETLRLCYRHIEKQRSSESL 321

Query: 307 RWLVDQTDHFPTDAALFLAKLIDGKLKPKPLINCSTQLEENIEEFLKEKKLILVVSKRLD 366
           + L    D    D    L  L+  K    PL +  T+ + ++ + L+ K ++L++S  L+
Sbjct: 322 KVLHSLFDTTHIDNMKILTALVHPKPHITPLQDGLTKRKVHL-DVLRRKTVLLLISD-LN 381

Query: 367 VSSEDLEILHLIYNEL---------KKENKYEMVWIPVISDPPNEGD-----EEAYKHLK 426
           +  ++L I   IY E          K    YE+VW+PV+ DP  + +     ++ ++ L+
Sbjct: 382 ILQDELSIFEQIYTESRRNLVGVDGKSHMPYEVVWVPVV-DPIEDFERSPILQKKFEDLR 441

Query: 427 SKMKWYVV--PFTTKVAGVRFLEEKWQLREDLLMVVLDSKSKIEFTNAIHLTRVWERDAI 486
             M WY V  P   +   V F+  +W      ++VV+D +      NA+H+  +W  +A 
Sbjct: 442 DPMPWYSVDSPKLIERHVVEFMRGRWHFMNKPILVVIDPQGNEASLNALHMIWIWGTEAF 501

Query: 487 PLTYERAKALLRRNWIESTVVKFSDQPRLQSWINQERNIIFYGGHNSKWVRQFEETAEAI 546
           P T  R + L RR      ++       + +WI  +  I  YGG +  W+R+F   A+A 
Sbjct: 502 PFTRSREEELWRRETFSLNLIVDGIDSVIFNWIKPDNYIFLYGGDDLDWIRRFTMAAKAT 561

Query: 547 KKDPWTREEGITFEMIPIGRRDKGE--------------------YDPAMMFRFWMAQRS 606
            KD       +  EM  +G+R+                        +PA+M+ FW    S
Sbjct: 562 AKD-----SNVNLEMAYVGKRNHSHREQIRRISEVIRSENLSHSWAEPALMWFFWTRLES 621

Query: 607 FFIIKHQLHGSNASEDV----SRLISYENEHGWAIIAKGPTVVFIGGGDLILKAMEEFQT 666
               K QL  ++  +DV     +++SY+   GWA+++KGP +V I  G +        +T
Sbjct: 622 MLYSKIQLGKADDHDDVMQGIKKILSYDKLGGWALLSKGPEIVMIAHGAIERTMSVYDRT 681

Query: 667 WKKNLRRGGFFSSFKDYF--DVLTAKSIHCTH--VNILGFSGWIPLIIICPVCRRYMGSG 680
           WK ++   G+  +  D+   +VL      C H   +I   SG IP  + C  C+R M   
Sbjct: 682 WKTHVPTKGYTKAMSDHHHDEVLRETGKPCGHFDFHITARSGRIPEKMNCFECQRPMEKY 728

BLAST of Sgr016489 vs. TAIR 10
Match: AT3G01670.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G01680.1); Has 121 Blast hits to 111 proteins in 12 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 121; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 161.0 bits (406), Expect = 3.4e-39
Identity = 174/743 (23.42%), Postives = 307/743 (41.32%), Query Frame = 0

Query: 22  RTLKHFSDD-VITHHIYTKHSDDETTKIDLNNYISFIENVIKTADQIAETHYGAQGSKGH 81
           R +   SDD V+   +   HS D     D+ + +S + ++ K+     +    +   K  
Sbjct: 131 RPMFSLSDDRVMADRVLKTHSPD-MIFFDVTSLLSVVNDIFKSHVPSID----SSAPKPS 190

Query: 82  LVFSD----DSLKYLSSSIDPPICTLHQISKELACK-------------APGIEGAHKTT 141
           LVF D     S +  +  ID       QIS E+ CK                ++  + TT
Sbjct: 191 LVFKDYADHTSFETFADLID-------QISCEIDCKCLHGGESHGMMTSGLHLDSRNTTT 250

Query: 142 LDILSKLTSYPWEAKAALTFTAFATDYGVMWHLEHYSQSDPLAKSLAMIKRVALLKKELG 201
             +LS ++ Y W+AK  L  +A A  YGV   L     ++ L KSLA+IK++        
Sbjct: 251 FSVLSLVSKYRWDAKLVLVLSALAVKYGVFLLLAETHATNQLTKSLALIKQLP------- 310

Query: 202 SFKYRHVFFDPNSLIYSCLQAMKYINELKNLSK--YDTKEVPE---LSAAVRQIPLVSYW 261
                 +F   N+L     +    + ++ +L+    D  ++P     +A    IP   YW
Sbjct: 311 -----SIFSRQNALHQRLDKTRILMQDMVDLTTTIIDIYQLPPNHITAAFTDHIPTAVYW 370

Query: 262 VIHTLVASSTELHSYLSGTDGQTQKYLNE--------------------LAEKFRSLLFT 321
           ++  ++       S++SG  G  Q  +                      L E+F+    T
Sbjct: 371 IVRCVLICV----SHISGASGFKQDQIMSFMEVSEIHENSERLRKINAYLLEQFKKSKMT 430

Query: 322 LEKHLQFIREQQEEVELYRWLVDQTDHFPTDAALFLAKLID------GKLKPKPLINCST 381
           +E+ +    E QE ++ +  ++   D  P    L L + ID      G  K +  IN  T
Sbjct: 431 IEEGI-IEEEYQELIQTFTTII-HVDVVP--PLLRLLRPIDFLYHGAGVSKRRVGINVLT 490

Query: 382 QLEENIEEFLKEKKLILVVSKRLDVSSEDLEILHLIYNELKKENKYEMVWIPVISDPPNE 441
           Q            K +L++   L+   ++L IL  +Y E  +++ +E++W+PV  D   E
Sbjct: 491 Q------------KHVLLLISDLENIEKELYILESLYTEAWQQS-FEILWVPV-QDFWTE 550

Query: 442 GDEEAYKHLKSKMKWYVV--PFTTKVAGVRFLEEKWQLREDLLMVVLDSKSKIEFTNAIH 501
            D+  ++ L   M+WYV+  P   + A +RF+ E W  +   ++V LD K ++  TNA  
Sbjct: 551 ADDAKFEALHMNMRWYVLGEPRKLRRAAIRFVREWWGFKNRPILVALDPKGQVMSTNAFP 610

Query: 502 LTRVWERDAIPLTYERAKAL-LRRNWIESTVVKFSDQPRLQSWINQERNIIFYGGHNSKW 561
           +  +W+  A P T  R + L   + W    ++  +D   L   ++  + I  YGG + +W
Sbjct: 611 MVWIWQPFAHPFTTARERDLWSEQEWNLEFLIDGTDPHSLNQLVD-GKYICLYGGEDMQW 670

Query: 562 VRQFEETAEAIKKDPWTREEGITFEMIPIGRRD-KGEYDPAM-----------------M 621
           ++ F      + K        I  EM+ +G+R+ K    P +                 +
Sbjct: 671 IKNFTSLWRNVAK-----AANIQLEMVYVGKRNPKNGIQPIINTIREENLSHTLPDLFQI 730

Query: 622 FRFWMAQRSFFIIKHQL---HGSNASE------------DVSRLISYENE-HGWAIIAKG 679
           + FW    S +  K ++   HG    E            +V  ++ Y  E  GW +++K 
Sbjct: 731 WFFWTRVESMWESKQRMLKAHGIKGREGFKEEEKDLVLQEVVAMLGYGGEGDGWGLVSKA 790

BLAST of Sgr016489 vs. TAIR 10
Match: AT1G67790.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G01680.1); Has 208 Blast hits to 125 proteins in 13 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 208; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 79.7 bits (195), Expect = 9.9e-15
Identity = 61/314 (19.43%), Postives = 124/314 (39.49%), Query Frame = 0

Query: 375 ENKYEMVWIPVISDPP-NEGDEEAYKHLKSKMKWYVV--PFTTKVAGVRFLEEKWQLRE- 434
           E  YE++W+P+ S     + ++E +    + + W  V  P+      + F +++W  ++ 
Sbjct: 269 EQNYEIIWVPIPSSQKWTDEEKEIFDFYSNSLPWISVRQPWLMSSTILNFFKQEWHYKDN 328

Query: 435 DLLMVVLDSKSKIEFTNAIHLTRVWERDAIPLTYERAKALLRRNWIESTVVKFSDQPRLQ 494
           + ++VV+DS  +    NA+ +  +W   A P +  R   L + +     ++     P  +
Sbjct: 329 EAMLVVIDSNGRFVNMNAMDMVLIWGVKAYPFSVSREDELWKEHGWSINLLLDGIHPTFE 388

Query: 495 SWINQERNIIFYGGHNSKWVRQFEETAEAIKKDPWTREEGITFEMIPIGRRDKGE----- 554
                 R I  +G  N  W+ +F   A  I      +  G   E+I +  + + E     
Sbjct: 389 G-----REICIFGSENLDWIDEFVSLARKI------QNLGFQLELIYLSNQRRDERAMEE 448

Query: 555 ----YDPAMMFRFWMAQRSF------FIIKHQLHGSNASEDVSRLI--SYENEHGWAIIA 614
               + P +   FW+   S        I+          E+V  L+   Y    GW II 
Sbjct: 449 SSILFSPTLQQLFWLRLESIERSKLKRIVIEPSKPDRVFEEVRNLLDFDYGKHRGWGIIG 508

Query: 615 KGPTVVFIGGGDLILKAMEEFQTWKKNLRRGGFFSSFKDYFDVLTAKSIHCTHVNILGFS 667
            G T   +  G+ + + M +   W +  +  G    F +  ++   K    +H  ++ F 
Sbjct: 509 NGSTAETV-DGEKMTERMRKIVRWGEYAKGLG----FTEAIEIAAEKPCELSHTAVVPFE 566