Sgr016404 (gene) Monk fruit (Qingpiguo) v1

Overview


Name	Sgr016404
Type	gene
Organism	Siraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description	Gibberellin 20-oxidase
Location	tig00152909: 478115 .. 478933 (-)
RNA-Seq Expression	Sgr016404
Synteny	Sgr016404

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGTGGGCTTTCGTTAGGGATCATGAAGCTTCTGGGTAAGAGCCTCGGCGTAGAAGAAGACTATTTCAAGAACTTCTTCACAGACAACGATTCAATAATGAGGTTGAACTTCTACCCGCCATGCCAGAAGCCCCATCTCACCTTAGGCACTGGCCCTCACTGCGACCCAACTTCCTTGACCATTCTTCACCAAGACCAAGTCGGTGGCTTGCAAGTTTTTGTAGACAACCAATGGAGAGTCATCGCCCCAAACTTTGATGCTTTTGTTGTCAATATCGGTGACACCTTCATGGTAATCAAACAAACCCCAAAAAAACAAAAACAAAAAAAGTTCATAAATTTTCTCTTCACTCCCCAATTTTTACCCTCCCACGTGTCATAGGTTAAGTATATTAGGTTTTAAAAACAAACCCAGTTAAGAATTAACGCTAAAAGTAACTTTTTAGAGTAAAGTTTAGTGTTTTAAAACAGAAGAGAAAAAGCAACCTTGGAATTTGCTAATTATGGATTTTTTTTGTTTTTGCATTAACGAAATTGGGTTTTGGATTGTGAAAGGCACTATCAAATGGGAGATACAAGAGTTGCTTGCATCGAGCAGTGGTGAACAGCGAGACAACAAGGAAATCTTTAGCATTTTTTTTGTGTCCAAGAAATGATAAAGTGGTGAAGCCACCGAGGGAGTTGGTGGACACTCAGAACCCACGAATATATCCAGATTTTACTTGGTCAATGCTGCTCGAATTCACACAGAAGCATTACAGAGCCGATATGAAAACCCTCGAAGTTTTCTCAGCCTGGATTCGTTCAACACAAGAAT

mRNA sequence

ATGAGTGGGCTTTCGTTAGGGATCATGAAGCTTCTGGGTAAGAGCCTCGGCGTAGAAGAAGACTATTTCAAGAACTTCTTCACAGACAACGATTCAATAATGAGGTTGAACTTCTACCCGCCATGCCAGAAGCCCCATCTCACCTTAGGCACTGGCCCTCACTGCGACCCAACTTCCTTGACCATTCTTCACCAAGACCAAGTCGGTGGCTTGCAAGTTTTTGTAGACAACCAATGGAGAGTCATCGCCCCAAACTTTGATGCTTTTGTTGTCAATATCGGTGACACCTTCATGGCACTATCAAATGGGAGATACAAGAGTTGCTTGCATCGAGCAGTGGTGAACAGCGAGACAACAAGGAAATCTTTAGCATTTTTTTTGTGTCCAAGAAATGATAAAGTGGTGAAGCCACCGAGGGAGTTGGTGGACACTCAGAACCCACGAATATATCCAGATTTTACTTGGTCAATGCTGCTCGAATTCACACAGAAGCATTACAGAGCCGATATGAAAACCCTCGAAGTTTTCTCAGCCTGGATTCGTTCAACACAAGAAT

Coding sequence (CDS)

ATGAGTGGGCTTTCGTTAGGGATCATGAAGCTTCTGGGTAAGAGCCTCGGCGTAGAAGAAGACTATTTCAAGAACTTCTTCACAGACAACGATTCAATAATGAGGTTGAACTTCTACCCGCCATGCCAGAAGCCCCATCTCACCTTAGGCACTGGCCCTCACTGCGACCCAACTTCCTTGACCATTCTTCACCAAGACCAAGTCGGTGGCTTGCAAGTTTTTGTAGACAACCAATGGAGAGTCATCGCCCCAAACTTTGATGCTTTTGTTGTCAATATCGGTGACACCTTCATGGCACTATCAAATGGGAGATACAAGAGTTGCTTGCATCGAGCAGTGGTGAACAGCGAGACAACAAGGAAATCTTTAGCATTTTTTTTGTGTCCAAGAAATGATAAAGTGGTGAAGCCACCGAGGGAGTTGGTGGACACTCAGAACCCACGAATATATCCAGATTTTACTTGGTCAATGCTGCTCGAATTCACACAGAAGCATTACAGAGCCGATATGAAAACCCTCGAAGTTTTCTCAGCCTGGATTCGTTCAACACAAGAAT

Protein sequence

MSGLSLGIMKLLGKSLGVEEDYFKNFFTDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSLTILHQDQVGGLQVFVDNQWRVIAPNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNSETTRKSLAFFLCPRNDKVVKPPRELVDTQNPRIYPDFTWSMLLEFTQKHYRADMKTLEVFSAWIRSTQEX

Homology

[Close]

BLAST of Sgr016404 vs. NCBI nr
Match: KAG7035591.1 (Gibberellin 20 oxidase 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 364.4 bits (934), Expect = 5.9e-97
Identity = 170/185 (91.89%), Postives = 175/185 (94.59%), Query Frame = 0


Query: 1   MSGLSLGIMKLLGKSLGVEEDYFKNFFTDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSL 60
           MSGLSLGIM+LLGKSLGVEE YFKNFF DNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSL
Sbjct: 185 MSGLSLGIMELLGKSLGVEEQYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSL 244

Query: 61  TILHQDQVGGLQVFVDNQWRVIAPNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNSETTR 120
           TILHQDQVGGLQVFVDNQWR+I PNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNSE TR
Sbjct: 245 TILHQDQVGGLQVFVDNQWRLITPNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNSERTR 304

Query: 121 KSLAFFLCPRNDKVVKPPRELVDTQNPRIYPDFTWSMLLEFTQKHYRADMKTLEVFSAWI 180
           KSLAFFLCPRNDKVV+PPRELVDTQNPR YPDFTWSMLL FTQ HYRADMKTLE FSAW+
Sbjct: 305 KSLAFFLCPRNDKVVRPPRELVDTQNPRRYPDFTWSMLLRFTQTHYRADMKTLEAFSAWL 364

Query: 181 RSTQE 186
           +  QE
Sbjct: 365 QQQQE 369

[Close]

BLAST of Sgr016404 vs. NCBI nr
Match: XP_022957867.1 (gibberellin 20 oxidase 1-like [Cucurbita moschata])

HSP 1 Score: 363.2 bits (931), Expect = 1.3e-96
Identity = 169/185 (91.35%), Postives = 175/185 (94.59%), Query Frame = 0


Query: 1   MSGLSLGIMKLLGKSLGVEEDYFKNFFTDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSL 60
           MSGLSLGIM+LLGKSLGVEE YFKNFF DNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSL
Sbjct: 185 MSGLSLGIMELLGKSLGVEEQYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSL 244

Query: 61  TILHQDQVGGLQVFVDNQWRVIAPNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNSETTR 120
           TILHQDQVGGLQVFVDNQWR+I PNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNSE TR
Sbjct: 245 TILHQDQVGGLQVFVDNQWRLITPNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNSERTR 304

Query: 121 KSLAFFLCPRNDKVVKPPRELVDTQNPRIYPDFTWSMLLEFTQKHYRADMKTLEVFSAWI 180
           KSLAFFLCPRNDKVV+PPRELVDTQNPR YPDFTWSMLL FTQ HYRADMKTLE FSAW+
Sbjct: 305 KSLAFFLCPRNDKVVRPPRELVDTQNPRRYPDFTWSMLLRFTQTHYRADMKTLEAFSAWL 364

Query: 181 RSTQE 186
           +  Q+
Sbjct: 365 QQQQQ 369

[Close]

BLAST of Sgr016404 vs. NCBI nr
Match: XP_023533923.1 (gibberellin 20 oxidase 1-like [Cucurbita pepo subsp. pepo] >XP_023533924.1 gibberellin 20 oxidase 1-like [Cucurbita pepo subsp. pepo] >XP_023533925.1 gibberellin 20 oxidase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 363.2 bits (931), Expect = 1.3e-96
Identity = 169/185 (91.35%), Postives = 175/185 (94.59%), Query Frame = 0


Query: 1   MSGLSLGIMKLLGKSLGVEEDYFKNFFTDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSL 60
           MSGLSLGIM+LLGKSLGVEE YFKNFF DNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSL
Sbjct: 185 MSGLSLGIMELLGKSLGVEEQYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSL 244

Query: 61  TILHQDQVGGLQVFVDNQWRVIAPNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNSETTR 120
           TILHQDQVGGLQVFVDNQWR+I PNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNSE TR
Sbjct: 245 TILHQDQVGGLQVFVDNQWRLITPNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNSERTR 304

Query: 121 KSLAFFLCPRNDKVVKPPRELVDTQNPRIYPDFTWSMLLEFTQKHYRADMKTLEVFSAWI 180
           KSLAFFLCPRNDKVV+PPRELVDTQNPR YPDFTWSMLL FTQ HYRADMKTLE FSAW+
Sbjct: 305 KSLAFFLCPRNDKVVRPPRELVDTQNPRRYPDFTWSMLLRFTQTHYRADMKTLEAFSAWL 364

Query: 181 RSTQE 186
           +  Q+
Sbjct: 365 QQQQQ 369

[Close]

BLAST of Sgr016404 vs. NCBI nr
Match: XP_023533927.1 (gibberellin 20 oxidase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 363.2 bits (931), Expect = 1.3e-96
Identity = 169/185 (91.35%), Postives = 175/185 (94.59%), Query Frame = 0


Query: 1   MSGLSLGIMKLLGKSLGVEEDYFKNFFTDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSL 60
           MSGLSLGIM+LLGKSLGVEE YFKNFF DNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSL
Sbjct: 1   MSGLSLGIMELLGKSLGVEEQYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSL 60

Query: 61  TILHQDQVGGLQVFVDNQWRVIAPNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNSETTR 120
           TILHQDQVGGLQVFVDNQWR+I PNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNSE TR
Sbjct: 61  TILHQDQVGGLQVFVDNQWRLITPNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNSERTR 120

Query: 121 KSLAFFLCPRNDKVVKPPRELVDTQNPRIYPDFTWSMLLEFTQKHYRADMKTLEVFSAWI 180
           KSLAFFLCPRNDKVV+PPRELVDTQNPR YPDFTWSMLL FTQ HYRADMKTLE FSAW+
Sbjct: 121 KSLAFFLCPRNDKVVRPPRELVDTQNPRRYPDFTWSMLLRFTQTHYRADMKTLEAFSAWL 180

Query: 181 RSTQE 186
           +  Q+
Sbjct: 181 QQQQQ 185

[Close]

BLAST of Sgr016404 vs. NCBI nr
Match: KAG6605683.1 (Gibberellin 20 oxidase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 363.2 bits (931), Expect = 1.3e-96
Identity = 169/185 (91.35%), Postives = 175/185 (94.59%), Query Frame = 0


Query: 1   MSGLSLGIMKLLGKSLGVEEDYFKNFFTDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSL 60
           MSGLSLGIM+LLGKSLGVEE YFKNFF DNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSL
Sbjct: 185 MSGLSLGIMELLGKSLGVEEQYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSL 244

Query: 61  TILHQDQVGGLQVFVDNQWRVIAPNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNSETTR 120
           TILHQDQVGGLQVFVDNQWR+I PNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNSE TR
Sbjct: 245 TILHQDQVGGLQVFVDNQWRLITPNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNSERTR 304

Query: 121 KSLAFFLCPRNDKVVKPPRELVDTQNPRIYPDFTWSMLLEFTQKHYRADMKTLEVFSAWI 180
           KSLAFFLCPRNDKVV+PPRELVDTQNPR YPDFTWSMLL FTQ HYRADMKTLE FSAW+
Sbjct: 305 KSLAFFLCPRNDKVVRPPRELVDTQNPRRYPDFTWSMLLRFTQTHYRADMKTLEAFSAWL 364

Query: 181 RSTQE 186
           +  Q+
Sbjct: 365 QQQQQ 369

[Close]

BLAST of Sgr016404 vs. ExPASy Swiss-Prot
Match: Q39110 (Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=GA20OX1 PE=2 SV=2)

HSP 1 Score: 300.8 bits (769), Expect = 1.0e-80
Identity = 141/180 (78.33%), Postives = 154/180 (85.56%), Query Frame = 0


Query: 1   MSGLSLGIMKLLGKSLGVEEDYFKNFFTDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSL 60
           MS LSL IM+LLG SLGV+ DYF+ FF +NDSIMRLN+YPPC KP LTLGTGPHCDPTSL
Sbjct: 194 MSSLSLKIMELLGLSLGVKRDYFREFFEENDSIMRLNYYPPCIKPDLTLGTGPHCDPTSL 253

Query: 61  TILHQDQVGGLQVFVDNQWRVIAPNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNSETTR 120
           TILHQD V GLQVFV+NQWR I PN  AFVVNIGDTFMALSN RYKSCLHRAVVNSE+ R
Sbjct: 254 TILHQDHVNGLQVFVENQWRSIRPNPKAFVVNIGDTFMALSNDRYKSCLHRAVVNSESER 313

Query: 121 KSLAFFLCPRNDKVVKPPRELVDTQNPRIYPDFTWSMLLEFTQKHYRADMKTLEVFSAWI 180
           KSLAFFLCP+ D+VV PPREL+D+   R YPDFTWSM LEFTQKHYRADM TL+ FS W+
Sbjct: 314 KSLAFFLCPKKDRVVTPPRELLDSITSRRYPDFTWSMFLEFTQKHYRADMNTLQAFSDWL 373

[Close]

BLAST of Sgr016404 vs. ExPASy Swiss-Prot
Match: O04706 (Gibberellin 20 oxidase 1-B OS=Triticum aestivum OX=4565 GN=GA20ox1B PE=2 SV=1)

HSP 1 Score: 292.7 bits (748), Expect = 2.9e-78
Identity = 138/185 (74.59%), Postives = 152/185 (82.16%), Query Frame = 0


Query: 1   MSGLSLGIMKLLGKSLGVEEDYFKNFFTDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSL 60
           MS LSL IM++LG+SLGV   +++ FF  NDSIMRLN+YPPCQ+P  TLGTGPHCDPTSL
Sbjct: 171 MSRLSLEIMEVLGESLGVGRAHYRRFFEGNDSIMRLNYYPPCQRPMETLGTGPHCDPTSL 230

Query: 61  TILHQDQVGGLQVFVDNQWRVIAPNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNSETTR 120
           TILHQD VGGLQV  + +WR I P  DAFVVNIGDTFMALSNGRYKSCLHRAVVNS+  R
Sbjct: 231 TILHQDNVGGLQVHTEGRWRSIRPRADAFVVNIGDTFMALSNGRYKSCLHRAVVNSKVPR 290

Query: 121 KSLAFFLCPRNDKVVKPPRELVDTQNPRIYPDFTWSMLLEFTQKHYRADMKTLEVFSAWI 180
           KSLAFFLCP  DKVV PP  LVD  NPR YPDFTW  LL+FTQKHYRADMKTLEVFS+WI
Sbjct: 291 KSLAFFLCPEMDKVVAPPGTLVDAANPRAYPDFTWRSLLDFTQKHYRADMKTLEVFSSWI 350

Query: 181 RSTQE 186
              Q+
Sbjct: 351 VQQQQ 355

[Close]

BLAST of Sgr016404 vs. ExPASy Swiss-Prot
Match: O04707 (Gibberellin 20 oxidase 1-A OS=Triticum aestivum OX=4565 GN=GA20ox1A PE=2 SV=1)

HSP 1 Score: 292.4 bits (747), Expect = 3.7e-78
Identity = 138/185 (74.59%), Postives = 151/185 (81.62%), Query Frame = 0


Query: 1   MSGLSLGIMKLLGKSLGVEEDYFKNFFTDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSL 60
           MS LSL IM++LG+SLGV   +++ FF  NDSIMRLN+YPPCQ+P  TLGTGPHCDPTSL
Sbjct: 171 MSRLSLEIMEVLGESLGVGRAHYRRFFEGNDSIMRLNYYPPCQRPLETLGTGPHCDPTSL 230

Query: 61  TILHQDQVGGLQVFVDNQWRVIAPNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNSETTR 120
           TILHQD VGGLQV  + +WR I P  DAFVVNIGDTFMALSNGRYKSCLHRAVVNS   R
Sbjct: 231 TILHQDNVGGLQVHTEGRWRSIRPRADAFVVNIGDTFMALSNGRYKSCLHRAVVNSRVPR 290

Query: 121 KSLAFFLCPRNDKVVKPPRELVDTQNPRIYPDFTWSMLLEFTQKHYRADMKTLEVFSAWI 180
           KSLAFFLCP  DKVV PP  LVD  NPR YPDFTW  LL+FTQKHYRADMKTLEVFS+WI
Sbjct: 291 KSLAFFLCPEMDKVVAPPGTLVDASNPRAYPDFTWRSLLDFTQKHYRADMKTLEVFSSWI 350

Query: 181 RSTQE 186
              Q+
Sbjct: 351 VQQQQ 355

[Close]

BLAST of Sgr016404 vs. ExPASy Swiss-Prot
Match: O04705 (Gibberellin 20 oxidase 1-D OS=Triticum aestivum OX=4565 GN=GA20ox1D PE=1 SV=1)

HSP 1 Score: 292.0 bits (746), Expect = 4.9e-78
Identity = 138/185 (74.59%), Postives = 151/185 (81.62%), Query Frame = 0


Query: 1   MSGLSLGIMKLLGKSLGVEEDYFKNFFTDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSL 60
           MS LSL IM++LG+SLGV   +++ FF  NDSIMRLN+YPPCQ+P  TLGTGPHCDPTSL
Sbjct: 171 MSRLSLEIMEVLGESLGVGRAHYRRFFEGNDSIMRLNYYPPCQRPLETLGTGPHCDPTSL 230

Query: 61  TILHQDQVGGLQVFVDNQWRVIAPNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNSETTR 120
           TILHQD VGGLQV  + +WR I P  DAFVVNIGDTFMALSNGRYKSCLHRAVVNS   R
Sbjct: 231 TILHQDNVGGLQVHTEGRWRSIRPRADAFVVNIGDTFMALSNGRYKSCLHRAVVNSRVPR 290

Query: 121 KSLAFFLCPRNDKVVKPPRELVDTQNPRIYPDFTWSMLLEFTQKHYRADMKTLEVFSAWI 180
           KSLAFFLCP  DKVV PP  LVD  NPR YPDFTW  LL+FTQKHYRADMKTLEVFS+WI
Sbjct: 291 KSLAFFLCPEMDKVVAPPGTLVDAANPRAYPDFTWRSLLDFTQKHYRADMKTLEVFSSWI 350

Query: 181 RSTQE 186
              Q+
Sbjct: 351 VQQQQ 355

[Close]

BLAST of Sgr016404 vs. ExPASy Swiss-Prot
Match: Q39111 (Gibberellin 20 oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=GA20OX2 PE=2 SV=1)

HSP 1 Score: 290.0 bits (741), Expect = 1.9e-77
Identity = 134/180 (74.44%), Postives = 150/180 (83.33%), Query Frame = 0


Query: 1   MSGLSLGIMKLLGKSLGVEEDYFKNFFTDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSL 60
           MS LSL IM+LLG SLGV  DYF+ FF +NDSIMRLN YPPCQ P LTLGTGPHCDP+SL
Sbjct: 192 MSSLSLKIMELLGLSLGVNRDYFRGFFEENDSIMRLNHYPPCQTPDLTLGTGPHCDPSSL 251

Query: 61  TILHQDQVGGLQVFVDNQWRVIAPNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNSETTR 120
           TILHQD V GLQVFVDNQW+ I PN  AFVVNIGDTFMALSNG +KSCLHRAVVN E+ R
Sbjct: 252 TILHQDHVNGLQVFVDNQWQSIRPNPKAFVVNIGDTFMALSNGIFKSCLHRAVVNRESAR 311

Query: 121 KSLAFFLCPRNDKVVKPPRELVDTQNPRIYPDFTWSMLLEFTQKHYRADMKTLEVFSAWI 180
           KS+AFFLCP+ DKVVKPP ++++    R YPDFTWSM LEFTQKHYRAD+ TL+ FS W+
Sbjct: 312 KSMAFFLCPKKDKVVKPPSDILEKMKTRKYPDFTWSMFLEFTQKHYRADVNTLDSFSNWV 371

[Close]

BLAST of Sgr016404 vs. ExPASy TrEMBL
Match: A0A6J1H0C4 (gibberellin 20 oxidase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111459279 PE=3 SV=1)

HSP 1 Score: 363.2 bits (931), Expect = 6.4e-97
Identity = 169/185 (91.35%), Postives = 175/185 (94.59%), Query Frame = 0


Query: 1   MSGLSLGIMKLLGKSLGVEEDYFKNFFTDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSL 60
           MSGLSLGIM+LLGKSLGVEE YFKNFF DNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSL
Sbjct: 185 MSGLSLGIMELLGKSLGVEEQYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSL 244

Query: 61  TILHQDQVGGLQVFVDNQWRVIAPNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNSETTR 120
           TILHQDQVGGLQVFVDNQWR+I PNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNSE TR
Sbjct: 245 TILHQDQVGGLQVFVDNQWRLITPNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNSERTR 304

Query: 121 KSLAFFLCPRNDKVVKPPRELVDTQNPRIYPDFTWSMLLEFTQKHYRADMKTLEVFSAWI 180
           KSLAFFLCPRNDKVV+PPRELVDTQNPR YPDFTWSMLL FTQ HYRADMKTLE FSAW+
Sbjct: 305 KSLAFFLCPRNDKVVRPPRELVDTQNPRRYPDFTWSMLLRFTQTHYRADMKTLEAFSAWL 364

Query: 181 RSTQE 186
           +  Q+
Sbjct: 365 QQQQQ 369

[Close]

BLAST of Sgr016404 vs. ExPASy TrEMBL
Match: A0A6J1EM68 (gibberellin 20 oxidase 1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111435674 PE=3 SV=1)

HSP 1 Score: 361.7 bits (927), Expect = 1.8e-96
Identity = 168/183 (91.80%), Postives = 176/183 (96.17%), Query Frame = 0


Query: 1   MSGLSLGIMKLLGKSLGVEEDYFKNFFTDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSL 60
           MSGLSLGIM+LLGKSLGVE DYFKNFF DNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSL
Sbjct: 178 MSGLSLGIMELLGKSLGVEGDYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSL 237

Query: 61  TILHQDQVGGLQVFVDNQWRVIAPNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNSETTR 120
           TILHQDQVGGLQVFVDNQWR+IAPNFDAFV+NIGDTFMALSNGRYKSCLHRAVVN+ETTR
Sbjct: 238 TILHQDQVGGLQVFVDNQWRLIAPNFDAFVINIGDTFMALSNGRYKSCLHRAVVNNETTR 297

Query: 121 KSLAFFLCPRNDKVVKPPRELVDTQNPRIYPDFTWSMLLEFTQKHYRADMKTLEVFSAWI 180
           KSLAFFLCPRNDKVV+PPRELVDTQNPRIYPDFTWSML  FT  +YRADMKTL+VFSAW+
Sbjct: 298 KSLAFFLCPRNDKVVRPPRELVDTQNPRIYPDFTWSMLHHFTLNNYRADMKTLDVFSAWL 357

Query: 181 RST 184
           R T
Sbjct: 358 RQT 360

[Close]

BLAST of Sgr016404 vs. ExPASy TrEMBL
Match: A0A6J1EQF6 (gibberellin 20 oxidase 1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111435674 PE=3 SV=1)

HSP 1 Score: 361.7 bits (927), Expect = 1.8e-96
Identity = 168/183 (91.80%), Postives = 176/183 (96.17%), Query Frame = 0


Query: 1   MSGLSLGIMKLLGKSLGVEEDYFKNFFTDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSL 60
           MSGLSLGIM+LLGKSLGVE DYFKNFF DNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSL
Sbjct: 179 MSGLSLGIMELLGKSLGVEGDYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSL 238

Query: 61  TILHQDQVGGLQVFVDNQWRVIAPNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNSETTR 120
           TILHQDQVGGLQVFVDNQWR+IAPNFDAFV+NIGDTFMALSNGRYKSCLHRAVVN+ETTR
Sbjct: 239 TILHQDQVGGLQVFVDNQWRLIAPNFDAFVINIGDTFMALSNGRYKSCLHRAVVNNETTR 298

Query: 121 KSLAFFLCPRNDKVVKPPRELVDTQNPRIYPDFTWSMLLEFTQKHYRADMKTLEVFSAWI 180
           KSLAFFLCPRNDKVV+PPRELVDTQNPRIYPDFTWSML  FT  +YRADMKTL+VFSAW+
Sbjct: 299 KSLAFFLCPRNDKVVRPPRELVDTQNPRIYPDFTWSMLHHFTLNNYRADMKTLDVFSAWL 358

Query: 181 RST 184
           R T
Sbjct: 359 RQT 361

[Close]

BLAST of Sgr016404 vs. ExPASy TrEMBL
Match: A0A6J1I242 (gibberellin 20 oxidase 1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111469145 PE=3 SV=1)

HSP 1 Score: 361.3 bits (926), Expect = 2.4e-96
Identity = 167/183 (91.26%), Postives = 176/183 (96.17%), Query Frame = 0


Query: 1   MSGLSLGIMKLLGKSLGVEEDYFKNFFTDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSL 60
           MSGLSLGIM+LLGKSLGV+ DYFKNFF DNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSL
Sbjct: 179 MSGLSLGIMELLGKSLGVKGDYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSL 238

Query: 61  TILHQDQVGGLQVFVDNQWRVIAPNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNSETTR 120
           TILHQDQVGGLQVFVDNQWR+IAPNFDAFV+NIGDTFMALSNGRYKSCLHRAVVN+ETTR
Sbjct: 239 TILHQDQVGGLQVFVDNQWRLIAPNFDAFVINIGDTFMALSNGRYKSCLHRAVVNNETTR 298

Query: 121 KSLAFFLCPRNDKVVKPPRELVDTQNPRIYPDFTWSMLLEFTQKHYRADMKTLEVFSAWI 180
           KSLAFFLCPRNDKVV+PPRELVDTQNPRIYPDFTWSML  FT  HYRADMKTL+VFSAW+
Sbjct: 299 KSLAFFLCPRNDKVVRPPRELVDTQNPRIYPDFTWSMLHHFTLNHYRADMKTLDVFSAWL 358

Query: 181 RST 184
           + T
Sbjct: 359 QQT 361

[Close]

BLAST of Sgr016404 vs. ExPASy TrEMBL
Match: A0A6J1HYD3 (gibberellin 20 oxidase 1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111469145 PE=3 SV=1)

HSP 1 Score: 361.3 bits (926), Expect = 2.4e-96
Identity = 167/183 (91.26%), Postives = 176/183 (96.17%), Query Frame = 0


Query: 1   MSGLSLGIMKLLGKSLGVEEDYFKNFFTDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSL 60
           MSGLSLGIM+LLGKSLGV+ DYFKNFF DNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSL
Sbjct: 178 MSGLSLGIMELLGKSLGVKGDYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSL 237

Query: 61  TILHQDQVGGLQVFVDNQWRVIAPNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNSETTR 120
           TILHQDQVGGLQVFVDNQWR+IAPNFDAFV+NIGDTFMALSNGRYKSCLHRAVVN+ETTR
Sbjct: 238 TILHQDQVGGLQVFVDNQWRLIAPNFDAFVINIGDTFMALSNGRYKSCLHRAVVNNETTR 297

Query: 121 KSLAFFLCPRNDKVVKPPRELVDTQNPRIYPDFTWSMLLEFTQKHYRADMKTLEVFSAWI 180
           KSLAFFLCPRNDKVV+PPRELVDTQNPRIYPDFTWSML  FT  HYRADMKTL+VFSAW+
Sbjct: 298 KSLAFFLCPRNDKVVRPPRELVDTQNPRIYPDFTWSMLHHFTLNHYRADMKTLDVFSAWL 357

Query: 181 RST 184
           + T
Sbjct: 358 QQT 360

[Close]

BLAST of Sgr016404 vs. TAIR 10
Match: AT4G25420.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 300.8 bits (769), Expect = 7.5e-82
Identity = 141/180 (78.33%), Postives = 154/180 (85.56%), Query Frame = 0


Query: 1   MSGLSLGIMKLLGKSLGVEEDYFKNFFTDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSL 60
           MS LSL IM+LLG SLGV+ DYF+ FF +NDSIMRLN+YPPC KP LTLGTGPHCDPTSL
Sbjct: 194 MSSLSLKIMELLGLSLGVKRDYFREFFEENDSIMRLNYYPPCIKPDLTLGTGPHCDPTSL 253

Query: 61  TILHQDQVGGLQVFVDNQWRVIAPNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNSETTR 120
           TILHQD V GLQVFV+NQWR I PN  AFVVNIGDTFMALSN RYKSCLHRAVVNSE+ R
Sbjct: 254 TILHQDHVNGLQVFVENQWRSIRPNPKAFVVNIGDTFMALSNDRYKSCLHRAVVNSESER 313

Query: 121 KSLAFFLCPRNDKVVKPPRELVDTQNPRIYPDFTWSMLLEFTQKHYRADMKTLEVFSAWI 180
           KSLAFFLCP+ D+VV PPREL+D+   R YPDFTWSM LEFTQKHYRADM TL+ FS W+
Sbjct: 314 KSLAFFLCPKKDRVVTPPRELLDSITSRRYPDFTWSMFLEFTQKHYRADMNTLQAFSDWL 373

[Close]

BLAST of Sgr016404 vs. TAIR 10
Match: AT5G51810.1 (gibberellin 20 oxidase 2 )

HSP 1 Score: 290.0 bits (741), Expect = 1.3e-78
Identity = 134/180 (74.44%), Postives = 150/180 (83.33%), Query Frame = 0


Query: 1   MSGLSLGIMKLLGKSLGVEEDYFKNFFTDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSL 60
           MS LSL IM+LLG SLGV  DYF+ FF +NDSIMRLN YPPCQ P LTLGTGPHCDP+SL
Sbjct: 192 MSSLSLKIMELLGLSLGVNRDYFRGFFEENDSIMRLNHYPPCQTPDLTLGTGPHCDPSSL 251

Query: 61  TILHQDQVGGLQVFVDNQWRVIAPNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNSETTR 120
           TILHQD V GLQVFVDNQW+ I PN  AFVVNIGDTFMALSNG +KSCLHRAVVN E+ R
Sbjct: 252 TILHQDHVNGLQVFVDNQWQSIRPNPKAFVVNIGDTFMALSNGIFKSCLHRAVVNRESAR 311

Query: 121 KSLAFFLCPRNDKVVKPPRELVDTQNPRIYPDFTWSMLLEFTQKHYRADMKTLEVFSAWI 180
           KS+AFFLCP+ DKVVKPP ++++    R YPDFTWSM LEFTQKHYRAD+ TL+ FS W+
Sbjct: 312 KSMAFFLCPKKDKVVKPPSDILEKMKTRKYPDFTWSMFLEFTQKHYRADVNTLDSFSNWV 371

[Close]

BLAST of Sgr016404 vs. TAIR 10
Match: AT5G07200.1 (gibberellin 20-oxidase 3 )

HSP 1 Score: 288.1 bits (736), Expect = 5.0e-78
Identity = 136/184 (73.91%), Postives = 153/184 (83.15%), Query Frame = 0


Query: 1   MSGLSLGIMKLLGKSLGVEEDYFKNFFTDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSL 60
           M+ LSL IM+LLG SLGVE  YFK FF D+DSI RLN+YP C++P L LGTGPHCDPTSL
Sbjct: 193 MNTLSLKIMELLGMSLGVERRYFKEFFEDSDSIFRLNYYPQCKQPELALGTGPHCDPTSL 252

Query: 61  TILHQDQVGGLQVFVDNQWRVIAPNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNSETTR 120
           TILHQDQVGGLQVFVDN+W+ I PN  AFVVNIGDTFMAL+NGRYKSCLHRAVVNSE  R
Sbjct: 253 TILHQDQVGGLQVFVDNKWQSIPPNPHAFVVNIGDTFMALTNGRYKSCLHRAVVNSERER 312

Query: 121 KSLAFFLCPRNDKVVKPPRELVD--TQNPRIYPDFTWSMLLEFTQKHYRADMKTLEVFSA 180
           K+ AFFLCP+ +KVVKPP ELV+      R YPDFTWSM LEFTQKHYRADM TL+ FS 
Sbjct: 313 KTFAFFLCPKGEKVVKPPEELVNGVKSGERKYPDFTWSMFLEFTQKHYRADMNTLDEFSI 372

Query: 181 WIRS 183
           W+++
Sbjct: 373 WLKN 376

[Close]

BLAST of Sgr016404 vs. TAIR 10
Match: AT1G60980.1 (gibberellin 20-oxidase 4 )

HSP 1 Score: 273.5 bits (698), Expect = 1.3e-73
Identity = 129/182 (70.88%), Postives = 148/182 (81.32%), Query Frame = 0


Query: 1   MSGLSLGIMKLLGKSLGVEEDYFKNFFTDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSL 60
           MS LSL IM+LLG SLG++ ++F+ FF DN+SI RLN+YP C++P L LGTGPHCDPTSL
Sbjct: 194 MSNLSLKIMELLGMSLGIKREHFREFFEDNESIFRLNYYPKCKQPDLVLGTGPHCDPTSL 253

Query: 61  TILHQDQVGGLQVFVDNQWRVIAPNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNSETTR 120
           TIL QDQV GLQVFVDNQW+ I P   A VVNIGDT MAL+NG YKSCLHRAVVN ETTR
Sbjct: 254 TILQQDQVSGLQVFVDNQWQSIPPIPQALVVNIGDTLMALTNGIYKSCLHRAVVNGETTR 313

Query: 121 KSLAFFLCPRNDKVVKPPRELVDTQNPRIYPDFTWSMLLEFTQKHYRADMKTLEVFSAWI 180
           K+LAFFLCP+ DKVVKPP EL   +  R YPDFTWSM LEFT KHYRADM TLE F+ W+
Sbjct: 314 KTLAFFLCPKVDKVVKPPSEL---EGERAYPDFTWSMFLEFTMKHYRADMNTLEEFTNWL 372

Query: 181 RS 183
           ++
Sbjct: 374 KN 372

[Close]

BLAST of Sgr016404 vs. TAIR 10
Match: AT1G44090.1 (gibberellin 20-oxidase 5 )

HSP 1 Score: 216.9 bits (551), Expect = 1.4e-56
Identity = 99/187 (52.94%), Postives = 130/187 (69.52%), Query Frame = 0


Query: 1   MSGLSLGIMKLLGKSLGVEE-DYFKNFFTDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTS 60
           M+GL + +M+LLG S+G+++  Y++ FF D   I R N+YPPC++P   LG GPH DPT+
Sbjct: 195 MNGLVMDLMELLGISMGLKDRTYYRRFFEDGSGIFRCNYYPPCKQPEKALGVGPHNDPTA 254

Query: 61  LTILHQDQVGGLQVFVDNQWRVIAPNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNSETT 120
           +T+L QD V GL+VF    W+ + P   A VVN+GDTFMALSNG Y+SC HRAVVN E  
Sbjct: 255 ITVLLQDDVVGLEVFAAGSWQTVRPRPGALVVNVGDTFMALSNGNYRSCYHRAVVNKEKV 314

Query: 121 RKSLAFFLCPRNDKVVKPPRELVDTQN-PRIYPDFTWSMLLEFTQKHYRADMKTLEVFSA 180
           R+SL FF CPR DK++ PP ELV+ +   R YPDFTW+ L +FTQ  YR D  TL  FS+
Sbjct: 315 RRSLVFFSCPREDKIIVPPPELVEGEEASRKYPDFTWAQLQKFTQSGYRVDNTTLHNFSS 374

Query: 181 WIRSTQE 186
           W+ S  +
Sbjct: 375 WLVSNSD 381

The following BLAST results are available for this feature:

BLAST of Sgr016404 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7035591.1	5.9e-97	91.89	Gibberellin 20 oxidase 1, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022957867.1	1.3e-96	91.35	gibberellin 20 oxidase 1-like [Cucurbita moschata]	[more]
XP_023533923.1	1.3e-96	91.35	gibberellin 20 oxidase 1-like [Cucurbita pepo subsp. pepo] >XP_023533924.1 gibbe...	[more]
XP_023533927.1	1.3e-96	91.35	gibberellin 20 oxidase 1-like [Cucurbita pepo subsp. pepo]	[more]
KAG6605683.1	1.3e-96	91.35	Gibberellin 20 oxidase 1, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Sgr016404 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q39110	1.0e-80	78.33	Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=GA20OX1 PE=2 SV=2	[more]
O04706	2.9e-78	74.59	Gibberellin 20 oxidase 1-B OS=Triticum aestivum OX=4565 GN=GA20ox1B PE=2 SV=1	[more]
O04707	3.7e-78	74.59	Gibberellin 20 oxidase 1-A OS=Triticum aestivum OX=4565 GN=GA20ox1A PE=2 SV=1	[more]
O04705	4.9e-78	74.59	Gibberellin 20 oxidase 1-D OS=Triticum aestivum OX=4565 GN=GA20ox1D PE=1 SV=1	[more]
Q39111	1.9e-77	74.44	Gibberellin 20 oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=GA20OX2 PE=2 SV=1	[more]

BLAST of Sgr016404 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H0C4	6.4e-97	91.35	gibberellin 20 oxidase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111459279 PE=3...	[more]
A0A6J1EM68	1.8e-96	91.80	gibberellin 20 oxidase 1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
A0A6J1EQF6	1.8e-96	91.80	gibberellin 20 oxidase 1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
A0A6J1I242	2.4e-96	91.26	gibberellin 20 oxidase 1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC11146...	[more]
A0A6J1HYD3	2.4e-96	91.26	gibberellin 20 oxidase 1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC11146...	[more]

BLAST of Sgr016404 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G25420.1	7.5e-82	78.33	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT5G51810.1	1.3e-78	74.44	gibberellin 20 oxidase 2	[more]
AT5G07200.1	5.0e-78	73.91	gibberellin 20-oxidase 3	[more]
AT1G60980.1	1.3e-73	70.88	gibberellin 20-oxidase 4	[more]
AT1G44090.1	1.4e-56	52.94	gibberellin 20-oxidase 5	[more]

InterPro

Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 1..168 e-value: 1.1E-59 score: 204.4
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 31..129 e-value: 3.3E-29 score: 101.4
None	No IPR available	PANTHER	PTHR47990:SF26	GIBBERELLIN 20-OXIDASE	coord: 1..181
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 1..181
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 1..169
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 29..129 score: 14.197751

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sgr016404.1	Sgr016404.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009908	flower development
biological_process	GO:0009686	gibberellin biosynthetic process
biological_process	GO:0009416	response to light stimulus
biological_process	GO:0009826	unidimensional cell growth
molecular_function	GO:0045544	gibberellin 20-oxidase activity
molecular_function	GO:0016707	gibberellin 3-beta-dioxygenase activity
molecular_function	GO:0046872	metal ion binding