Sgr016281 (gene) Monk fruit (Qingpiguo) v1

Overview


Name	Sgr016281
Type	gene
Organism	Siraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description	Plant invertase/pectin methylesterase inhibitor superfamily
Location	tig00007935: 645064 .. 650677 (+)
RNA-Seq Expression	Sgr016281
Synteny	Sgr016281

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATTCATCATCTGTTCTTGTTTTTACAATTTTACTTATTTGTTCTGCGGCAGCCAATGTTATGAATCCGAGGATGAAGGTGGCGAATGATTTTGTTCGAGATACATGCAACAAAACTGTAATGAAAGAGCTTTGCATTTCTGTGGTGAATTCAGACGTTCGTGAAGATTTGAAATCAAACTTGACTGGTCTTCTCACCATATTCGTCAACCAAACTCTCCATGCCGTCATTGATAATCATTCTTATCTTTACAATGAAATAAGAAATGGACAATTGTCGAATCGAACATATCAGGTCTTTACTGTATGCTTGGATCAATATCAAATGAGTAAAGATTCTTTAGGACATATATTGGAAGAAAAACTATTGAAGGAACATAATACAGATTTGAATATGGATTTGTTCCAAATCGATAGCTATCTAACTCTTTGTGAGGATGATTTTGTGGAGTTTACACCAGAACCCTCAGCTTGGAAGTCTCGCTATGATTCTGTGGATTCGCTTCTAGTTCTTTCATTGGAAATGACAAATCTCATCACATGTGGTGATGTTCATCAACCTGTTTGCTAGGCTAGCCAACTTTTGAGATTGGTTTTTGCTATTATGAGTATTTAACTTTTTTGGCTGTTGCCCCCCTTGTTTTTTCACTTCAAGAAACGGTTTAATTTGAGTTCAGTGTTGTCACCATTTCAATGTGCATTGATAATCTCTCTTTTCATTTATAACGTTTTCACATCTTAATTCTTCCAAGCAAATAAAACTCTCACACGTCTTTACTTCAAATATATGGAGAAATACTTCTCAGGTATAATAAGAAAATGACTGAAACGGAGGGGTTTTCACCCAATTATTATGAATATTAACCCTTTCTTTTACACGACATGTAACTGTTGCCCCAATAATTTTTTTCTGATTTCATTTATGCAGTTCTCACCTCAGGTTTATGTATTGTTTATATAAACCTCATGTACTTCAAGATACTTTTACAGCACATTTTAAACTTATCATTTTTATGATTTGATTATGCAAATGAAATGATATTGGAAGTAAAATTTGACGAGCACTTATATTCTATTATAATTAATAATATATTCTTTTTATTTGTTAAATTATTGATATTACCCTTCTAATATTATATTAAAAATAAGAAAGAGGAAGTGTTGAAGTTTTAGAGCCAGGGTTTATGTTAAAGAATTGTACATCCTAATTCCTCTTAAAATGCATGTGAGAAAGAGAGAGAGAGAGAGATTGTGACCTCAATAACAGGAAACGAAAATCAATTACAGAGAAAGAGAGAGAAATAATTATCAGCCTTTCAAGGAAAAAGAATTGTAGAAAATTTGAGCAGGCTTTAATACTAAATGAACAAGTCGATTAGCCAATTCGAATATAGCTTAGTAGATAAGACACCTATTACTATTAGCTTTCTTGAGATTGAATGTTCGATCTCCCACCTCACATTTATTGAATTAAAAAAATAAAAAAATTAGTCGATTCATGACTTGTTAACAATTTAATTCCATCATAAACTGTTCCAAACGTTGTTATCTAGAGTATGTACAACCCAGAACAAACTCAAGACTAAACAATTTGATAATAACATGGTTCTTTACATTTTCTTTAGAATGAATGTTTACTCGCAATATTTCAAATAAAGGGCAATTATTTGTTTTGTTTGGAAAGAAAGTATCATTGCAAATAAATGGAATGAAATATTGCTCATTTAAAAGAATATATTATTTTGAAGATCACTAATTAATACCCGTATCATGTCCTATACAATATAAAATCATTGCATATATGTATAATGCTTTCAAAAAATATATTTATATGGTAATTAAGGCAATAACAATTTCTTATGTCTAGGAAATGATACAACCTTTTAGTGATATTCACAATAACTTTTTTAATATATATTTCATTCCTTTATTGCAAATGCAAGTAAAAAAGTTATTTCCAAACAAAATGGCTCCTTATCTTCCATATAAATATGCAACACACCCGGTGTTTTCTCATAGTAGAAGGGCAAGTTTTGAGAGAAAAGATGAATCTATCACCTGCTCTTGTTTTTACAATTTAACTTGTTTGCTCTGCATCAGCCAATACTATGGATCCGAAGAAGAATGTGGTGAATGATTTTGTTCGCGACACATGCAACAAAACTTAAGATTTGAAACAACTTTGCATTTTTGTGGTGGATTCAATACTCGCGAAGATTTGAAATTAAATATTTGACTGGTCTTCTAGCCATATTCATCCATCAAATGTGGTCATCACGTTCATCAATCCGTTTGTTAGGCAAGCCAACTTTAGAGATTGGTTTTTGCTGTCATTAACGTGAGACTTTAGTTGTATTTCCAACAATATAGCAAGAAAAGTCTACTTTTTTTTTTTTTTTGGATTCTATAATTAAAGAACCAAATAAAATCATAAAAATTTGATTGTGAAAGAATCAAAATAATTATCAGATCATTTTTGAAAACAAGGTCTTTGCAACCATGCCATAAAATTTACATGGATTAAATACTTTTGAACATGGATGTAGAAAAAATGGAATGGAGAAGCAAGCAAAATAATGAAAATAACAACAGTGAGAGCCAATAGAAAACAGATGATGAAAATTTGGAAAGTTGTAAATAATTATGATTGGATGTCCAAAATCAATCTTGTGTTCTATTACAGAAAACATAATTTTTTTTTCAAATCTTGAGTTCTGTTGCAAGTTGTTTTCAACGAAACTGCTTGCTAATTTCATATCTATAGGTTAACTGTGGACCAATTTCCATAAAACAGCAGTAGGTGATGACAGATTAATGATAATTTTGCTCGTTTTTTGTTTTCTAACTCAAATTGGAGAATTACGTATGAATGAAGGAAATTAATATTGCCATCTTTGAATTAATGGTTTGATATCCTTAGTTTTTATTTTTCTTTTTTGACGGAGATATCCTCGATTCTGTGTTTGGCAATTAGGAGAAAATTTTCTTATAAACCAAAGATGAAATATGAAATATTGTTTTAGAAAAGTCACTAAAAAAAAAAAAAACCTATCCCAATGAAAAGAAACAAAGTCAGGTTAGGTTTGTGACATTTTTGAAAAAAAAAAAAAAGTACTGACATTTTTTAAACTACATATAATATTTTTGCTATCTAATGTTTTCATATTCTTGTTTCTAGATTTTTGTTTTCTTTTTTTTCATCTTTAAAGCTTATCATTGAGGATTATATATATTTTTCACAGTTATTGAAAGTATTTTTTATTTGATTAATTTATATCATCAATAAACTTATAGAAGATTATTGACGATGTTCTTGTTGTTTTAGAAATATTATCTAGACATAGGTGTAGCTGAAAGAATGCTCTGAATAGATAAGGTTATTGTTATTATTTTTTTAGTACAAGTTTACTTTGCTTAATCATATGATATAACATATGACCTTAAATAAGATATACCTCTCTATGATGGATTCTAGTGTGGTGTTCTATTAAGAGCATAGTGTGGTCACGAAATCACAATATTGAATTCGTTATATATTATAATAAACTGAAGCTAGATCACTGATCTAATAATGTAATTTCAAATTATTACAGTATACTCGGCCTTCAAAATCTACTTTATTTTGTAACTGCAATATCGCACTGTAGCTCTTAATTGGAGTTCTCTTATACAAGCCTCCTTCAATGTATGTACCTTACATACCTTTCACTTAAGCGGTTTCTTACAAAATATATATATATATGAAGAATGTATATTATATGGGTTGTATACTTTTGGAGGCATATGTTACATATTATATTAATGAATACTCATACCCGTATTCAAGATGATGCATTACTACCAAAGTTCCAATGTGATCTATTATATATAAATATTAAACTTCTTTGGTATTAATCTAGAGATGCAATGTTTGGGAAAATTAATGATATGATAATTGCTTTGATTATATCTCCAAAATCAAATTTCTTTTCAACTTTGGACATCACTTTCCCTTAAAAAGTTTTATTAAATTCATATTTATAACTATAACTTAGAGATCAACTTGCTTGAGCTTTTGAATATGCATGCAGCAGGCATATGGGAAATCTTGTAATTATTATGGGGGCGATGTTAAAGGTAACTCGGCAGCTTAAAATATTCTTCTGATTGACCATCAAATGTCATAATCTGCAGTAAAAAGGAATCAGCTAGCAAAAAAAGTGAGAGATCAGGTCAATTATAGACCAAAAGAAACATAGGGTTTCAGTCGAAAGTTTATATAATTTGCTTCTACCATATGTGTGTGTATGAAAGGAATAGGGAGAGAAGATTACTAACCATTAATGAAACTGGCCAATATTATTCCTTGCCTATTTATATATATGTTGAAAACGTGTTTCTCTTGGCTATCAATTATGTTCTTTGAACAAGATGACATTGGCTGATGTAGTTACTTTTAGTAGGTTTTTTTTTTTTTTATAATTGTTTTAGTTTTTTAAAATACAAATTCCTCTGTAAATAGAGGCTTTTAGCTTTTGTTTCACACATCTTCATAATCAAATTTCATTTTGTGAGTAACATTGCTCTTGAGAGCTTTAGGCTACAACATTGAGAGAGCCTCTTAAACTTGGCTTTCAGAGAGTTCAAATGCAACCAATTGCTCCTCTCTCTCTCTCCCTCACAACCCTAACATGGCAGTGCAAATTGTTATGTGTTATTTTATGTTCTTAGCACTCCTCCTCAATCATTCTGTTGAACTTCCTCACTTTGATTGGAGATGATGCGGCTGCCACCATTATCGTCGACAATCGAAGTAATCATGCAGAGTTTTCAACCACATCTACTGCACACTCTGTAAGGCCATATCTTCCATTAACATTACATGAAAATAATGTAAGTGTTTTTTATTTGCTAATGGACATGTTTAATTTGTAGCTGTCAAGGCTAATAACTTCATGGCCCAGTTCGTAACTTTTCAAAACTCTGCCGGACCAGAAGATGGGCAAGCAGTAGCAGTTCTTGATGAAGCTAACCACACAGCTTACTACAGGTGTGTGTTTTCAAGTTATCAAGACACTCTCTATGTAAGAGGAATGTTTCAGTTCTTCAAGGAGTCCGATATATATGGTAGTGTCGACTTCATTTTTGGAGATGGGCTCGTCGTATTTCAAGACTGTAATATATATGCTCGATTATTAGACACTATATTAACTGCACAGTCCCAATCCTACGTGGACGAACGGAGCGGCTTCACCTTCCAAAACTGCAATGTGACAGTGTCATCGGAGATATCGTCAAGCAAGGACAAGGTTAAGGTGTCTCTTGGACGACCATGGAGAAACTACTCTACAGTCATTTTTATGGAGTCCTTTGTTGATAATGTAGTTCAACCAAGTGGATGGTCGGAATGGTCAGGAACACTGGTCGAACTATTATTTTACGCAGAATACAACAATAGTGGTCCGGGAGCTGACACTTGTAAACGGGTGACTTGGCCTGGTTATCATGTATTGCACGATGAAGTTGAAGCTGCAAATTTTACGGTTGAAAACTTCATTGATGGACTAAATGGCTGCCAGAGACTGGCATACCATTTAGAACTGGTTTGCATGCATGAGCACTGCATGCCCTAA

mRNA sequence

ATGGATTCATCATCTGTTCTTGTTTTTACAATTTTACTTATTTGTTCTGCGGCAGCCAATGTTATGAATCCGAGGATGAAGGTGGCGAATGATTTTGTTCGAGATACATGCAACAAAACTGTAATGAAAGAGCTTTGCATTTCTGTGGTGAATTCAGACGTTCGTGAAGATTTGAAATCAAACTTGACTGGTCTTCTCACCATATTCGTCAACCAAACTCTCCATGCCGTCATTGATAATCATTCTTATCTTTACAATGAAATAAGAAATGGACAATTGTCGAATCGAACATATCAGGTCTTTACTGTATGCTTGGATCAATATCAAATGAGTAAAGATTCTTTAGGACATATATTGGAAGAAAAACTATTGAAGGAACATAATACAGATTTGAATATGGATTTGTTCCAAATCGATAGCTATCTAACTCTTTGTGAGGATGATTTTGTGGAGTTTACACCAGAACCCTCAGCTTGGAAGTCTCGCTATGATTCTGTGGATTCGCTTCTAGTTCTTTCATTGGAAATGACAAATCTCATCACATGTGGTGATGTTCATCAACCTCACTCCTCCTCAATCATTCTGTTGAACTTCCTCACTTTGATTGGAGATGATGCGGCTGCCACCATTATCGTCGACAATCGAACTGTCAAGGCTAATAACTTCATGGCCCAGTTCGTAACTTTTCAAAACTCTGCCGGACCAGAAGATGGGCAAGCAGTAGCAGTTCTTGATGAAGCTAACCACACAGCTTACTACAGGTGTGTGTTTTCAAGTTATCAAGACACTCTCTATGTAAGAGGAATGTTTCAGTTCTTCAAGGAGTCCGATATATATGGTAGTGTCGACTTCATTTTTGGAGATGGGCTCGTCGTATTTCAAGACTGTAATATATATGCTCGATTATTAGACACTATATTAACTGCACAGTCCCAATCCTACGTGGACGAACGGAGCGGCTTCACCTTCCAAAACTGCAATGTGACAGTGTCATCGGAGATATCGTCAAGCAAGGACAAGGTTAAGGTGTCTCTTGGACGACCATGGAGAAACTACTCTACAGTCATTTTTATGGAGTCCTTTGTTGATAATGTAGTTCAACCAAGTGGATGGTCGGAATGGTCAGGAACACTGGTCGAACTATTATTTTACGCAGAATACAACAATAGTGGTCCGGGAGCTGACACTTGTAAACGGGTGACTTGGCCTGGTTATCATGTATTGCACGATGAAGTTGAAGCTGCAAATTTTACGGTTGAAAACTTCATTGATGGACTAAATGGCTGCCAGAGACTGGCATACCATTTAGAACTGGTTTGCATGCATGAGCACTGCATGCCCTAA

Coding sequence (CDS)

ATGGATTCATCATCTGTTCTTGTTTTTACAATTTTACTTATTTGTTCTGCGGCAGCCAATGTTATGAATCCGAGGATGAAGGTGGCGAATGATTTTGTTCGAGATACATGCAACAAAACTGTAATGAAAGAGCTTTGCATTTCTGTGGTGAATTCAGACGTTCGTGAAGATTTGAAATCAAACTTGACTGGTCTTCTCACCATATTCGTCAACCAAACTCTCCATGCCGTCATTGATAATCATTCTTATCTTTACAATGAAATAAGAAATGGACAATTGTCGAATCGAACATATCAGGTCTTTACTGTATGCTTGGATCAATATCAAATGAGTAAAGATTCTTTAGGACATATATTGGAAGAAAAACTATTGAAGGAACATAATACAGATTTGAATATGGATTTGTTCCAAATCGATAGCTATCTAACTCTTTGTGAGGATGATTTTGTGGAGTTTACACCAGAACCCTCAGCTTGGAAGTCTCGCTATGATTCTGTGGATTCGCTTCTAGTTCTTTCATTGGAAATGACAAATCTCATCACATGTGGTGATGTTCATCAACCTCACTCCTCCTCAATCATTCTGTTGAACTTCCTCACTTTGATTGGAGATGATGCGGCTGCCACCATTATCGTCGACAATCGAACTGTCAAGGCTAATAACTTCATGGCCCAGTTCGTAACTTTTCAAAACTCTGCCGGACCAGAAGATGGGCAAGCAGTAGCAGTTCTTGATGAAGCTAACCACACAGCTTACTACAGGTGTGTGTTTTCAAGTTATCAAGACACTCTCTATGTAAGAGGAATGTTTCAGTTCTTCAAGGAGTCCGATATATATGGTAGTGTCGACTTCATTTTTGGAGATGGGCTCGTCGTATTTCAAGACTGTAATATATATGCTCGATTATTAGACACTATATTAACTGCACAGTCCCAATCCTACGTGGACGAACGGAGCGGCTTCACCTTCCAAAACTGCAATGTGACAGTGTCATCGGAGATATCGTCAAGCAAGGACAAGGTTAAGGTGTCTCTTGGACGACCATGGAGAAACTACTCTACAGTCATTTTTATGGAGTCCTTTGTTGATAATGTAGTTCAACCAAGTGGATGGTCGGAATGGTCAGGAACACTGGTCGAACTATTATTTTACGCAGAATACAACAATAGTGGTCCGGGAGCTGACACTTGTAAACGGGTGACTTGGCCTGGTTATCATGTATTGCACGATGAAGTTGAAGCTGCAAATTTTACGGTTGAAAACTTCATTGATGGACTAAATGGCTGCCAGAGACTGGCATACCATTTAGAACTGGTTTGCATGCATGAGCACTGCATGCCCTAA

Protein sequence

MDSSSVLVFTILLICSAAANVMNPRMKVANDFVRDTCNKTVMKELCISVVNSDVREDLKSNLTGLLTIFVNQTLHAVIDNHSYLYNEIRNGQLSNRTYQVFTVCLDQYQMSKDSLGHILEEKLLKEHNTDLNMDLFQIDSYLTLCEDDFVEFTPEPSAWKSRYDSVDSLLVLSLEMTNLITCGDVHQPHSSSIILLNFLTLIGDDAAATIIVDNRTVKANNFMAQFVTFQNSAGPEDGQAVAVLDEANHTAYYRCVFSSYQDTLYVRGMFQFFKESDIYGSVDFIFGDGLVVFQDCNIYARLLDTILTAQSQSYVDERSGFTFQNCNVTVSSEISSSKDKVKVSLGRPWRNYSTVIFMESFVDNVVQPSGWSEWSGTLVELLFYAEYNNSGPGADTCKRVTWPGYHVLHDEVEAANFTVENFIDGLNGCQRLAYHLELVCMHEHCMP

Homology

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BLAST of Sgr016281 vs. NCBI nr
Match: XP_022148123.1 (probable pectinesterase/pectinesterase inhibitor 39 [Momordica charantia])

HSP 1 Score: 370.2 bits (949), Expect = 2.6e-98
Identity = 177/256 (69.14%), Postives = 207/256 (80.86%), Query Frame = 0


Query: 185 VHQPHSSSIILLNFLTLIGDDAAATIIVDNR--------------TVKANNFMAQFVTFQ 244
           +++ H     L  F++LIGDDA+ T+IVD+R              TVKA +F+AQF+TFQ
Sbjct: 67  IYKEHIEVPELKTFISLIGDDASTTVIVDDRSNHAGFSTTSSATFTVKAKHFVAQFLTFQ 126

Query: 245 NSAGPEDGQAVAVLDEANHTAYYRCVFSSYQDTLYVRGMFQFFKESDIYGSVDFIFGDGL 304
           NSAGPE+GQAVAVL EAN+TAYY+CVF  YQDTLYVRG FQ FKE D+YGS+DFIFGDGL
Sbjct: 127 NSAGPENGQAVAVLSEANYTAYYKCVFMGYQDTLYVRGAFQLFKECDVYGSIDFIFGDGL 186

Query: 305 VVFQDCNIYARLLDTILTAQSQSYVDERSGFTFQNCNVTVSSEISSSKDKVKVSLGRPWR 364
            +FQDCN+YAR+L T +TAQS+SY D+RSGFTFQNCNVTVS EI S KD+V VSLGRPWR
Sbjct: 187 AIFQDCNVYARILGTTITAQSKSYKDQRSGFTFQNCNVTVSPEIQSIKDQVTVSLGRPWR 246

Query: 365 NYSTVIFMESFVDNVVQPSGWSEWSGTLVELLFYAEYNNSGPGADTCKRVTWPGYHVLHD 424
           NYSTVIFMESF+D++V+P GWSEW  T V LLFYAEYNNSG GADT +RV WPGYHVLH+
Sbjct: 247 NYSTVIFMESFLDDIVEPGGWSEWPRTPVYLLFYAEYNNSGSGADTSQRVKWPGYHVLHN 306

Query: 425 EVEAANFTVENFIDGL 427
           E EA+ FTVE FIDGL
Sbjct: 307 EDEASRFTVEIFIDGL 322

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BLAST of Sgr016281 vs. NCBI nr
Match: XP_022150332.1 (probable pectinesterase/pectinesterase inhibitor 39 [Momordica charantia])

HSP 1 Score: 334.7 bits (857), Expect = 1.2e-87
Identity = 162/257 (63.04%), Postives = 196/257 (76.26%), Query Frame = 0


Query: 185 VHQPHSSSIILLNFLTLIGDDAAATIIVDNRT--------------VKANNFMAQFVTFQ 244
           +++ H    +   F+ LIG+DA+ TIIVDNR+              VK  NFMAQF+TF+
Sbjct: 67  IYKEHVEVPVQKTFIALIGEDASTTIIVDNRSNGSGFSTQTTATLIVKGQNFMAQFLTFE 126

Query: 245 NSAGPEDGQAVAVLDEANHTAYYRCVFSSYQDTLYVRGMFQFFKESDIYGSVDFIFGDGL 304
           NSA  ++GQA+AV+D+A HTAYY+CVF SYQDTLY   + QFFKE DIYG +DFIFG+GL
Sbjct: 127 NSAEQKEGQAIAVMDQAKHTAYYKCVFLSYQDTLYAGAVPQFFKECDIYGRIDFIFGNGL 186

Query: 305 VVFQDCNIYARLLDT--ILTAQSQSYVDERSGFTFQNCNVTVSSEISSSKDKVKVSLGRP 364
           VVFQDCNIYAR+  T  I+TAQS++ V+++SGF FQNCNVTVS EI+SSKD V + LGRP
Sbjct: 187 VVFQDCNIYARIPGTHIIVTAQSKNAVEDQSGFIFQNCNVTVSPEIASSKDNVTMFLGRP 246

Query: 365 WRNYSTVIFMESFVDNVVQPSGWSEWSGTLVELLFYAEYNNSGPGADTCKRVTWPGYHVL 424
           WR+YS VIFMESF+D VVQP GW EW G  V LLFYAEYNN GPGADT +RV WPGYHVL
Sbjct: 247 WRDYSMVIFMESFLDRVVQPKGWLEWPGRPVNLLFYAEYNNRGPGADTSQRVDWPGYHVL 306

Query: 425 HDEVEAANFTVENFIDG 426
           H+ +E   FTVENFI+G
Sbjct: 307 HNAMEVTKFTVENFING 323

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BLAST of Sgr016281 vs. NCBI nr
Match: XP_023538248.1 (probable pectinesterase/pectinesterase inhibitor 39 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 331.6 bits (849), Expect = 1.0e-86
Identity = 164/257 (63.81%), Postives = 193/257 (75.10%), Query Frame = 0


Query: 185 VHQPHSSSIILLNFLTLIGDDAAATIIVDNR--------------TVKANNFMAQFVTFQ 244
           ++  H        F++LIGD+A+ TIIVDNR              TV   NFMA+F+TFQ
Sbjct: 65  IYNEHIEVPFQKTFISLIGDNASTTIIVDNRSKATGFSTANSATLTVNGANFMAKFLTFQ 124

Query: 245 NSAGPEDGQAVAVLDEANHTAYYRCVFSSYQDTLYVRGMFQFFKESDIYGSVDFIFGDGL 304
           NSAGP+ GQAVAVLD+A HTAYY+CVF  YQDTLY   + QFFKE DIYG+VDFIFG+GL
Sbjct: 125 NSAGPKMGQAVAVLDQAKHTAYYKCVFLGYQDTLYAAAVPQFFKECDIYGTVDFIFGNGL 184

Query: 305 VVFQDCNIYARLLD--TILTAQSQSYVDERSGFTFQNCNVTVSSEISSSKDKVKVSLGRP 364
           VVFQDCNIYARL+D  T +TAQS++ +DE+SGF FQNC VTVS EI+SS+DKV V LGRP
Sbjct: 185 VVFQDCNIYARLVDIHTTVTAQSKANLDEQSGFVFQNCTVTVSPEIASSRDKVLVFLGRP 244

Query: 365 WRNYSTVIFMESFVDNVVQPSGWSEWSGTLVELLFYAEYNNSGPGADTCKRVTWPGYHVL 424
           WR YS V F+ESF+DNVVQP GW EW G  V LL+Y EYNN G GA+T +RV WPG+HVL
Sbjct: 245 WRAYSMVAFVESFLDNVVQPKGWLEWLGVPVNLLYYGEYNNRGGGANTSQRVDWPGFHVL 304

Query: 425 HDEVEAANFTVENFIDG 426
           H   E ANFTVENFI+G
Sbjct: 305 HSLTEVANFTVENFING 321

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BLAST of Sgr016281 vs. NCBI nr
Match: XP_022951717.1 (probable pectinesterase/pectinesterase inhibitor 39 [Cucurbita moschata])

HSP 1 Score: 330.5 bits (846), Expect = 2.3e-86
Identity = 163/257 (63.42%), Postives = 193/257 (75.10%), Query Frame = 0


Query: 185 VHQPHSSSIILLNFLTLIGDDAAATIIVDNR--------------TVKANNFMAQFVTFQ 244
           ++  H        F++LIGD+A+ TIIVDNR              TV   NFMA+F+TFQ
Sbjct: 65  IYNEHIEVPFQKTFISLIGDNASTTIIVDNRSKAAGFSTSNSATLTVNGANFMAKFLTFQ 124

Query: 245 NSAGPEDGQAVAVLDEANHTAYYRCVFSSYQDTLYVRGMFQFFKESDIYGSVDFIFGDGL 304
           NSAGP+ GQAVAVLD+A HTAYY+CVF  YQDTLY   + QFFKE DIYG+VDFIFG+GL
Sbjct: 125 NSAGPKMGQAVAVLDQAKHTAYYKCVFLGYQDTLYAAAVPQFFKECDIYGTVDFIFGNGL 184

Query: 305 VVFQDCNIYARLLD--TILTAQSQSYVDERSGFTFQNCNVTVSSEISSSKDKVKVSLGRP 364
           VVFQDCNIYARL+D  T +TAQS++ +DE+SGF FQNC VTVS EI+SS+DKV V LGRP
Sbjct: 185 VVFQDCNIYARLVDIHTTVTAQSKANLDEQSGFVFQNCTVTVSPEIASSRDKVLVFLGRP 244

Query: 365 WRNYSTVIFMESFVDNVVQPSGWSEWSGTLVELLFYAEYNNSGPGADTCKRVTWPGYHVL 424
           WR YS V F+ESF+DNVVQP GW EW G  V LL+Y EYNN G GA+T +RV WPG+HVL
Sbjct: 245 WRAYSMVAFVESFLDNVVQPKGWLEWLGVPVNLLYYGEYNNRGGGANTSQRVDWPGFHVL 304

Query: 425 HDEVEAANFTVENFIDG 426
           H   + ANFTVENFI+G
Sbjct: 305 HSLTDVANFTVENFING 321

[Close]

BLAST of Sgr016281 vs. NCBI nr
Match: KAG6585822.1 (putative pectinesterase/pectinesterase inhibitor 39, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 328.9 bits (842), Expect = 6.6e-86
Identity = 162/257 (63.04%), Postives = 193/257 (75.10%), Query Frame = 0


Query: 185 VHQPHSSSIILLNFLTLIGDDAAATIIVDNR--------------TVKANNFMAQFVTFQ 244
           ++  H        F++LIGD+A+ TIIVDNR              TV   NFMA+F+TFQ
Sbjct: 65  IYNEHIEVPFQKTFISLIGDNASTTIIVDNRSKAAGFSTANSATLTVNGANFMAKFLTFQ 124

Query: 245 NSAGPEDGQAVAVLDEANHTAYYRCVFSSYQDTLYVRGMFQFFKESDIYGSVDFIFGDGL 304
           NSAGP+ GQAVAVLD+A HTAYY+CVF  +QDTLY   + QFFKE DIYG+VDFIFG+GL
Sbjct: 125 NSAGPKMGQAVAVLDQAKHTAYYKCVFLGFQDTLYAAAVPQFFKECDIYGTVDFIFGNGL 184

Query: 305 VVFQDCNIYARLLD--TILTAQSQSYVDERSGFTFQNCNVTVSSEISSSKDKVKVSLGRP 364
           VVFQDCNIYARL+D  T +TAQS++ +DE+SGF FQNC VTVS EI+SS+DKV V LGRP
Sbjct: 185 VVFQDCNIYARLVDIHTTVTAQSKANLDEQSGFVFQNCTVTVSPEIASSRDKVLVFLGRP 244

Query: 365 WRNYSTVIFMESFVDNVVQPSGWSEWSGTLVELLFYAEYNNSGPGADTCKRVTWPGYHVL 424
           WR YS V F+ESF+DNVVQP GW EW G  V LL+Y EYNN G GA+T +RV WPG+HVL
Sbjct: 245 WRAYSMVAFVESFLDNVVQPKGWLEWLGVPVNLLYYGEYNNRGGGANTSQRVDWPGFHVL 304

Query: 425 HDEVEAANFTVENFIDG 426
           H   + ANFTVENFI+G
Sbjct: 305 HSLTDVANFTVENFING 321

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BLAST of Sgr016281 vs. ExPASy Swiss-Prot
Match: Q8RXK7 (Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3702 GN=PME41 PE=2 SV=2)

HSP 1 Score: 226.1 bits (575), Expect = 7.9e-58
Identity = 110/247 (44.53%), Postives = 156/247 (63.16%), Query Frame = 0


Query: 198 FLTLIGDDAAATIIVDNR--------------TVKANNFMAQFVTFQNSAGPEDGQAVAV 257
           +L +IGD    T++  NR               V + NF+A  +TF+N+AGPE  QAVA+
Sbjct: 311 YLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNTAGPEKHQAVAM 370

Query: 258 LDEANHTAYYRCVFSSYQDTLYVRGMFQFFKESDIYGSVDFIFGDGLVVFQDCNIYARL- 317
              A+ + +Y C F +YQDTLY   + QF++E DIYG+VDFIFG+  VVFQDCN+Y R  
Sbjct: 371 RSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQDCNLYPRQP 430

Query: 318 ---LDTILTAQSQSYVDERSGFTFQNCNVTVSSEISSSKDKVKVSLGRPWRNYSTVIFME 377
                  +TAQ ++  ++ +G +  NC +  + ++ SS   VK  LGRPW+ YS  +FM+
Sbjct: 431 MQNQFNAITAQGRTDPNQNTGISIHNCTIKPADDLVSSNYTVKTYLGRPWKEYSRTVFMQ 490

Query: 378 SFVDNVVQPSGWSEWSGTL-VELLFYAEYNNSGPGADTCKRVTWPGYHVLHDEVEAANFT 426
           S++D VV+P GW EW+G   +  L+YAEYNN+G G+ T  RV WPGYHV+ +  +A NFT
Sbjct: 491 SYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHVI-NSTDANNFT 550

[Close]

BLAST of Sgr016281 vs. ExPASy Swiss-Prot
Match: Q9SRX4 (Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PME7 PE=2 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 2.3e-57
Identity = 116/301 (38.54%), Postives = 177/301 (58.80%), Query Frame = 0


Query: 138 IDSYLTLCEDDFVEFTPEPSAWKSRYDSVDSLLVLSLEMTNLITCGDVHQPHSSSIILLN 197
           I   +T+ +D    FT   +A  +  ++ D     S     +     +++ + S      
Sbjct: 261 ISDIVTVSQDGTGNFTNITAAVAAAPNNTDG----SAGFFLIYVTAGIYEEYISIAKNKR 320

Query: 198 FLTLIGDDAAATIIVDNRT--------------VKANNFMAQFVTFQNSAGPEDGQAVAV 257
           ++ +IGD    T++  NR+              V A NF+A  +TF+N+AGPE  QAVA+
Sbjct: 321 YMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNITFRNTAGPEKHQAVAL 380

Query: 258 LDEANHTAYYRCVFSSYQDTLYVRGMFQFFKESDIYGSVDFIFGDGLVVFQDCNIYARL- 317
              A+ + +Y C F +YQDTLY   + QF++E D+YG+VDFIFG+  VVFQ+CN+Y R  
Sbjct: 381 RSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVFQNCNLYPRKP 440

Query: 318 ---LDTILTAQSQSYVDERSGFTFQNCNVTVSSEISSSKDKVKVSLGRPWRNYSTVIFME 377
                  +TAQ +S  ++ +G + QNC +  + ++ SS   VK  LGRPW+ YS  ++M+
Sbjct: 441 MPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDLVSSNYTVKTYLGRPWKEYSRTVYMQ 500

Query: 378 SFVDNVVQPSGWSEWSGTL-VELLFYAEYNNSGPGADTCKRVTWPGYHVLHDEVEAANFT 420
           S++D  V+P GW EW+G   +  L+YAEYNN+GPG++T  RVTWPGYHV+ +  +AANFT
Sbjct: 501 SYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYHVI-NSTDAANFT 556

[Close]

BLAST of Sgr016281 vs. ExPASy Swiss-Prot
Match: O48711 (Probable pectinesterase/pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3702 GN=PME12 PE=2 SV=1)

HSP 1 Score: 223.4 bits (568), Expect = 5.1e-57
Identity = 109/246 (44.31%), Postives = 154/246 (62.60%), Query Frame = 0


Query: 199 LTLIGDDAAATIIVDNRT--------------VKANNFMAQFVTFQNSAGPEDGQAVAVL 258
           + LIGD +  T I  NR+              V    F+A+ +   N+AGPE  QAVA+ 
Sbjct: 288 IVLIGDGSDVTFITGNRSVGDGWTTFRSATLAVSGEGFLARDIMITNTAGPEKHQAVALR 347

Query: 259 DEANHTAYYRCVFSSYQDTLYVRGMFQFFKESDIYGSVDFIFGDGLVVFQDCNIYARL-- 318
             A+  A YRCV   YQDTLY     QF++E DIYG++D+IFG+  VVFQ CNI ++L  
Sbjct: 348 VNADFVALYRCVIDGYQDTLYTHSFRQFYRECDIYGTIDYIFGNAAVVFQGCNIVSKLPM 407

Query: 319 --LDTILTAQSQSYVDERSGFTFQNCNVTVSSEISSSKDKVKVSLGRPWRNYSTVIFMES 378
               T++TAQS+   DE +G + QNC++  S ++ +S +KVK  LGRPWR +S  + MES
Sbjct: 408 PGQFTVITAQSRDTQDEDTGISMQNCSILASEDLFNSSNKVKSYLGRPWREFSRTVVMES 467

Query: 379 FVDNVVQPSGWSEWS-GTLVELLFYAEYNNSGPGADTCKRVTWPGYHVLHDEVEAANFTV 426
           ++D  +  SGWS+W+ G  ++ L+Y EYNN+GPG++T KRV WPG+H++  E +A NFT 
Sbjct: 468 YIDEFIDGSGWSKWNGGEALDTLYYGEYNNNGPGSETVKRVNWPGFHIMGYE-DAFNFTA 527

[Close]

BLAST of Sgr016281 vs. ExPASy Swiss-Prot
Match: O04887 (Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 8.2e-55
Identity = 104/250 (41.60%), Postives = 154/250 (61.60%), Query Frame = 0


Query: 194 ILLNFLTLIGDDAAATIIVDNRT--------------VKANNFMAQFVTFQNSAGPEDGQ 253
           + L  +  +GD    TII  +++              V  +NF+A+ +T +N+AGP + Q
Sbjct: 246 VKLKNIMFVGDGIGKTIITGSKSVGGGATTFKSATVAVVGDNFIARDITIRNTAGPNNHQ 305

Query: 254 AVAVLDEANHTAYYRCVFSSYQDTLYVRGMFQFFKESDIYGSVDFIFGDGLVVFQDCNIY 313
           AVA+   ++ + +YRC F  YQDTLYV    QF++E DIYG+VDFIFG+  VV Q+CNI+
Sbjct: 306 AVALRSGSDLSVFYRCSFEGYQDTLYVHSQRQFYRECDIYGTVDFIFGNAAVVLQNCNIF 365

Query: 314 ARL---LDTILTAQSQSYVDERSGFTFQNCNVTVSSEISSSKDKVKVSLGRPWRNYSTVI 373
           AR        LTAQ ++  ++ +G    NC VT +S++   +  VK  LGRPW+ YS  +
Sbjct: 366 ARKPPNRTNTLTAQGRTDPNQSTGIIIHNCRVTAASDLKPVQSSVKTFLGRPWKQYSRTV 425

Query: 374 FMESFVDNVVQPSGWSEWSGTL-VELLFYAEYNNSGPGADTCKRVTWPGYHVLHDEVEAA 426
           ++++F+D+++ P+GW EWSG   +  L+YAEY N+GPG+ T  RV W GYHVL    + +
Sbjct: 426 YIKTFLDSLINPAGWMEWSGDFALNTLYYAEYMNTGPGSSTANRVKWRGYHVLTSPSQVS 485

[Close]

BLAST of Sgr016281 vs. ExPASy Swiss-Prot
Match: O22256 (Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3702 GN=PME20 PE=2 SV=2)

HSP 1 Score: 214.9 bits (546), Expect = 1.8e-54
Identity = 101/247 (40.89%), Postives = 153/247 (61.94%), Query Frame = 0


Query: 198 FLTLIGDDAAATIIVDNRTV--------------KANNFMAQFVTFQNSAGPEDGQAVAV 257
           ++ +IGD    T+I  NR+V                 NF+   +T +N+AGP  GQAVA+
Sbjct: 298 YVMMIGDGINQTVITGNRSVVDGWTTFNSATFILSGPNFIGVNITIRNTAGPTKGQAVAL 357

Query: 258 LDEANHTAYYRCVFSSYQDTLYVRGMFQFFKESDIYGSVDFIFGDGLVVFQDCNIYARL- 317
               + + +Y C F +YQDTLY   + QF++E D+YG+VDFIFG+  VV Q+CN+Y R  
Sbjct: 358 RSGGDLSVFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNLYPRQP 417

Query: 318 ---LDTILTAQSQSYVDERSGFTFQNCNVTVSSEISSSKDKVKVSLGRPWRNYSTVIFME 377
                  +TAQ ++  ++ +G     C +  + ++++S   VK  LGRPW+ YS  + M+
Sbjct: 418 RKGQSNEVTAQGRTDPNQNTGTAIHGCTIRPADDLATSNYTVKTYLGRPWKEYSRTVVMQ 477

Query: 378 SFVDNVVQPSGWSEWSGTL-VELLFYAEYNNSGPGADTCKRVTWPGYHVLHDEVEAANFT 426
           +++D  ++PSGW+ WSG   +  L+YAEYNN+GPG+DT  RVTWPGYHV+ +  +A+NFT
Sbjct: 478 TYIDGFLEPSGWNAWSGDFALSTLYYAEYNNTGPGSDTTNRVTWPGYHVI-NATDASNFT 537

[Close]

BLAST of Sgr016281 vs. ExPASy TrEMBL
Match: A0A6J1D381 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111016876 PE=4 SV=1)

HSP 1 Score: 370.2 bits (949), Expect = 1.2e-98
Identity = 177/256 (69.14%), Postives = 207/256 (80.86%), Query Frame = 0


Query: 185 VHQPHSSSIILLNFLTLIGDDAAATIIVDNR--------------TVKANNFMAQFVTFQ 244
           +++ H     L  F++LIGDDA+ T+IVD+R              TVKA +F+AQF+TFQ
Sbjct: 67  IYKEHIEVPELKTFISLIGDDASTTVIVDDRSNHAGFSTTSSATFTVKAKHFVAQFLTFQ 126

Query: 245 NSAGPEDGQAVAVLDEANHTAYYRCVFSSYQDTLYVRGMFQFFKESDIYGSVDFIFGDGL 304
           NSAGPE+GQAVAVL EAN+TAYY+CVF  YQDTLYVRG FQ FKE D+YGS+DFIFGDGL
Sbjct: 127 NSAGPENGQAVAVLSEANYTAYYKCVFMGYQDTLYVRGAFQLFKECDVYGSIDFIFGDGL 186

Query: 305 VVFQDCNIYARLLDTILTAQSQSYVDERSGFTFQNCNVTVSSEISSSKDKVKVSLGRPWR 364
            +FQDCN+YAR+L T +TAQS+SY D+RSGFTFQNCNVTVS EI S KD+V VSLGRPWR
Sbjct: 187 AIFQDCNVYARILGTTITAQSKSYKDQRSGFTFQNCNVTVSPEIQSIKDQVTVSLGRPWR 246

Query: 365 NYSTVIFMESFVDNVVQPSGWSEWSGTLVELLFYAEYNNSGPGADTCKRVTWPGYHVLHD 424
           NYSTVIFMESF+D++V+P GWSEW  T V LLFYAEYNNSG GADT +RV WPGYHVLH+
Sbjct: 247 NYSTVIFMESFLDDIVEPGGWSEWPRTPVYLLFYAEYNNSGSGADTSQRVKWPGYHVLHN 306

Query: 425 EVEAANFTVENFIDGL 427
           E EA+ FTVE FIDGL
Sbjct: 307 EDEASRFTVEIFIDGL 322

[Close]

BLAST of Sgr016281 vs. ExPASy TrEMBL
Match: A0A6J1DAG5 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111018528 PE=4 SV=1)

HSP 1 Score: 334.7 bits (857), Expect = 5.8e-88
Identity = 162/257 (63.04%), Postives = 196/257 (76.26%), Query Frame = 0


Query: 185 VHQPHSSSIILLNFLTLIGDDAAATIIVDNRT--------------VKANNFMAQFVTFQ 244
           +++ H    +   F+ LIG+DA+ TIIVDNR+              VK  NFMAQF+TF+
Sbjct: 67  IYKEHVEVPVQKTFIALIGEDASTTIIVDNRSNGSGFSTQTTATLIVKGQNFMAQFLTFE 126

Query: 245 NSAGPEDGQAVAVLDEANHTAYYRCVFSSYQDTLYVRGMFQFFKESDIYGSVDFIFGDGL 304
           NSA  ++GQA+AV+D+A HTAYY+CVF SYQDTLY   + QFFKE DIYG +DFIFG+GL
Sbjct: 127 NSAEQKEGQAIAVMDQAKHTAYYKCVFLSYQDTLYAGAVPQFFKECDIYGRIDFIFGNGL 186

Query: 305 VVFQDCNIYARLLDT--ILTAQSQSYVDERSGFTFQNCNVTVSSEISSSKDKVKVSLGRP 364
           VVFQDCNIYAR+  T  I+TAQS++ V+++SGF FQNCNVTVS EI+SSKD V + LGRP
Sbjct: 187 VVFQDCNIYARIPGTHIIVTAQSKNAVEDQSGFIFQNCNVTVSPEIASSKDNVTMFLGRP 246

Query: 365 WRNYSTVIFMESFVDNVVQPSGWSEWSGTLVELLFYAEYNNSGPGADTCKRVTWPGYHVL 424
           WR+YS VIFMESF+D VVQP GW EW G  V LLFYAEYNN GPGADT +RV WPGYHVL
Sbjct: 247 WRDYSMVIFMESFLDRVVQPKGWLEWPGRPVNLLFYAEYNNRGPGADTSQRVDWPGYHVL 306

Query: 425 HDEVEAANFTVENFIDG 426
           H+ +E   FTVENFI+G
Sbjct: 307 HNAMEVTKFTVENFING 323

[Close]

BLAST of Sgr016281 vs. ExPASy TrEMBL
Match: A0A6J1GIG0 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111454459 PE=4 SV=1)

HSP 1 Score: 330.5 bits (846), Expect = 1.1e-86
Identity = 163/257 (63.42%), Postives = 193/257 (75.10%), Query Frame = 0


Query: 185 VHQPHSSSIILLNFLTLIGDDAAATIIVDNR--------------TVKANNFMAQFVTFQ 244
           ++  H        F++LIGD+A+ TIIVDNR              TV   NFMA+F+TFQ
Sbjct: 65  IYNEHIEVPFQKTFISLIGDNASTTIIVDNRSKAAGFSTSNSATLTVNGANFMAKFLTFQ 124

Query: 245 NSAGPEDGQAVAVLDEANHTAYYRCVFSSYQDTLYVRGMFQFFKESDIYGSVDFIFGDGL 304
           NSAGP+ GQAVAVLD+A HTAYY+CVF  YQDTLY   + QFFKE DIYG+VDFIFG+GL
Sbjct: 125 NSAGPKMGQAVAVLDQAKHTAYYKCVFLGYQDTLYAAAVPQFFKECDIYGTVDFIFGNGL 184

Query: 305 VVFQDCNIYARLLD--TILTAQSQSYVDERSGFTFQNCNVTVSSEISSSKDKVKVSLGRP 364
           VVFQDCNIYARL+D  T +TAQS++ +DE+SGF FQNC VTVS EI+SS+DKV V LGRP
Sbjct: 185 VVFQDCNIYARLVDIHTTVTAQSKANLDEQSGFVFQNCTVTVSPEIASSRDKVLVFLGRP 244

Query: 365 WRNYSTVIFMESFVDNVVQPSGWSEWSGTLVELLFYAEYNNSGPGADTCKRVTWPGYHVL 424
           WR YS V F+ESF+DNVVQP GW EW G  V LL+Y EYNN G GA+T +RV WPG+HVL
Sbjct: 245 WRAYSMVAFVESFLDNVVQPKGWLEWLGVPVNLLYYGEYNNRGGGANTSQRVDWPGFHVL 304

Query: 425 HDEVEAANFTVENFIDG 426
           H   + ANFTVENFI+G
Sbjct: 305 HSLTDVANFTVENFING 321

[Close]

BLAST of Sgr016281 vs. ExPASy TrEMBL
Match: A0A6J1KPG6 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111496427 PE=4 SV=1)

HSP 1 Score: 326.2 bits (835), Expect = 2.1e-85
Identity = 160/244 (65.57%), Postives = 189/244 (77.46%), Query Frame = 0


Query: 198 FLTLIGDDAAATIIVDNR--------------TVKANNFMAQFVTFQNSAGPEDGQAVAV 257
           F++LIGD+A+ TIIVDNR              TV   NFMA+F+TFQNSAGP+ GQAVAV
Sbjct: 78  FISLIGDNASTTIIVDNRSKATGFSTANSATLTVNGANFMAKFLTFQNSAGPKMGQAVAV 137

Query: 258 LDEANHTAYYRCVFSSYQDTLYVRGMFQFFKESDIYGSVDFIFGDGLVVFQDCNIYARLL 317
           LD+A HTAYY+CVF  YQDTLY   + QFFKE DIYG+VDFIFG+GLVVFQDCNIYARL+
Sbjct: 138 LDQAKHTAYYKCVFLGYQDTLYAAAVPQFFKECDIYGTVDFIFGNGLVVFQDCNIYARLV 197

Query: 318 D--TILTAQSQSYVDERSGFTFQNCNVTVSSEISSSKDKVKVSLGRPWRNYSTVIFMESF 377
           D  + +TAQS++ +DE+SGF FQNC VTVS EI+SS+DKV V LGRPWR YS V F+ESF
Sbjct: 198 DIQSTVTAQSKANLDEQSGFVFQNCTVTVSPEIASSRDKVLVFLGRPWRAYSMVAFVESF 257

Query: 378 VDNVVQPSGWSEWSGTLVELLFYAEYNNSGPGADTCKRVTWPGYHVLHDEVEAANFTVEN 426
           +DNVVQP GW EW G  V LL+Y EYNN G GA+T +RV WPG+HVLH   + A FTVEN
Sbjct: 258 LDNVVQPKGWLEWLGVPVNLLYYGEYNNRGGGANTSQRVDWPGFHVLHSLTDVAKFTVEN 317

[Close]

BLAST of Sgr016281 vs. ExPASy TrEMBL
Match: A0A6J1DCN7 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111019286 PE=4 SV=1)

HSP 1 Score: 321.2 bits (822), Expect = 6.7e-84
Identity = 158/255 (61.96%), Postives = 188/255 (73.73%), Query Frame = 0


Query: 185 VHQPHSSSIILLNFLTLIGDDAAATIIVDNR--------------TVKANNFMAQFVTFQ 244
           +++ H    +   F+ LIGDDA+ TIIVDNR              TVK  NFMAQ +TFQ
Sbjct: 67  IYKEHVEVPVQKTFIALIGDDASTTIIVDNRSNGSGFSTSATATLTVKGQNFMAQSLTFQ 126

Query: 245 NSAGPEDGQAVAVLDEANHTAYYRCVFSSYQDTLYVRGMFQFFKESDIYGSVDFIFGDGL 304
           NSAGP + QA+A+LD+A HTAYY+CVF  YQDTLY   + QFFKE DIYGSVDFIFG+GL
Sbjct: 127 NSAGPNEHQAIALLDQAKHTAYYKCVFLGYQDTLYAAAVPQFFKECDIYGSVDFIFGNGL 186

Query: 305 VVFQDCNIYARLLDTI-LTAQSQSYVDERSGFTFQNCNVTVSSEISSSKDKVKVSLGRPW 364
            VFQDCNIYA+L   I +TAQS+  V++ SGF FQ CNVTVS  I+SS+DKV + LGRPW
Sbjct: 187 AVFQDCNIYAQLSGIITVTAQSKDKVNDHSGFIFQRCNVTVSPNIASSRDKVIMFLGRPW 246

Query: 365 RNYSTVIFMESFVDNVVQPSGWSEWSGTLVELLFYAEYNNSGPGADTCKRVTWPGYHVLH 424
           R YS V+F+ES++DNVVQP GW EW G  V LLFYAEY NSGPGA T +RV WPGYHVL+
Sbjct: 247 RTYSMVVFIESYLDNVVQPKGWLEWPGVPVNLLFYAEYKNSGPGAHTPQRVHWPGYHVLY 306

[Close]

BLAST of Sgr016281 vs. TAIR 10
Match: AT4G02330.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 226.1 bits (575), Expect = 5.6e-59
Identity = 110/247 (44.53%), Postives = 156/247 (63.16%), Query Frame = 0


Query: 198 FLTLIGDDAAATIIVDNR--------------TVKANNFMAQFVTFQNSAGPEDGQAVAV 257
           +L +IGD    T++  NR               V + NF+A  +TF+N+AGPE  QAVA+
Sbjct: 311 YLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNTAGPEKHQAVAM 370

Query: 258 LDEANHTAYYRCVFSSYQDTLYVRGMFQFFKESDIYGSVDFIFGDGLVVFQDCNIYARL- 317
              A+ + +Y C F +YQDTLY   + QF++E DIYG+VDFIFG+  VVFQDCN+Y R  
Sbjct: 371 RSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQDCNLYPRQP 430

Query: 318 ---LDTILTAQSQSYVDERSGFTFQNCNVTVSSEISSSKDKVKVSLGRPWRNYSTVIFME 377
                  +TAQ ++  ++ +G +  NC +  + ++ SS   VK  LGRPW+ YS  +FM+
Sbjct: 431 MQNQFNAITAQGRTDPNQNTGISIHNCTIKPADDLVSSNYTVKTYLGRPWKEYSRTVFMQ 490

Query: 378 SFVDNVVQPSGWSEWSGTL-VELLFYAEYNNSGPGADTCKRVTWPGYHVLHDEVEAANFT 426
           S++D VV+P GW EW+G   +  L+YAEYNN+G G+ T  RV WPGYHV+ +  +A NFT
Sbjct: 491 SYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHVI-NSTDANNFT 550

[Close]

BLAST of Sgr016281 vs. TAIR 10
Match: AT1G02810.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 224.6 bits (571), Expect = 1.6e-58
Identity = 116/301 (38.54%), Postives = 177/301 (58.80%), Query Frame = 0


Query: 138 IDSYLTLCEDDFVEFTPEPSAWKSRYDSVDSLLVLSLEMTNLITCGDVHQPHSSSIILLN 197
           I   +T+ +D    FT   +A  +  ++ D     S     +     +++ + S      
Sbjct: 261 ISDIVTVSQDGTGNFTNITAAVAAAPNNTDG----SAGFFLIYVTAGIYEEYISIAKNKR 320

Query: 198 FLTLIGDDAAATIIVDNRT--------------VKANNFMAQFVTFQNSAGPEDGQAVAV 257
           ++ +IGD    T++  NR+              V A NF+A  +TF+N+AGPE  QAVA+
Sbjct: 321 YMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNITFRNTAGPEKHQAVAL 380

Query: 258 LDEANHTAYYRCVFSSYQDTLYVRGMFQFFKESDIYGSVDFIFGDGLVVFQDCNIYARL- 317
              A+ + +Y C F +YQDTLY   + QF++E D+YG+VDFIFG+  VVFQ+CN+Y R  
Sbjct: 381 RSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVFQNCNLYPRKP 440

Query: 318 ---LDTILTAQSQSYVDERSGFTFQNCNVTVSSEISSSKDKVKVSLGRPWRNYSTVIFME 377
                  +TAQ +S  ++ +G + QNC +  + ++ SS   VK  LGRPW+ YS  ++M+
Sbjct: 441 MPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDLVSSNYTVKTYLGRPWKEYSRTVYMQ 500

Query: 378 SFVDNVVQPSGWSEWSGTL-VELLFYAEYNNSGPGADTCKRVTWPGYHVLHDEVEAANFT 420
           S++D  V+P GW EW+G   +  L+YAEYNN+GPG++T  RVTWPGYHV+ +  +AANFT
Sbjct: 501 SYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYHVI-NSTDAANFT 556

[Close]

BLAST of Sgr016281 vs. TAIR 10
Match: AT2G26440.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 223.4 bits (568), Expect = 3.6e-58
Identity = 109/246 (44.31%), Postives = 154/246 (62.60%), Query Frame = 0


Query: 199 LTLIGDDAAATIIVDNRT--------------VKANNFMAQFVTFQNSAGPEDGQAVAVL 258
           + LIGD +  T I  NR+              V    F+A+ +   N+AGPE  QAVA+ 
Sbjct: 288 IVLIGDGSDVTFITGNRSVGDGWTTFRSATLAVSGEGFLARDIMITNTAGPEKHQAVALR 347

Query: 259 DEANHTAYYRCVFSSYQDTLYVRGMFQFFKESDIYGSVDFIFGDGLVVFQDCNIYARL-- 318
             A+  A YRCV   YQDTLY     QF++E DIYG++D+IFG+  VVFQ CNI ++L  
Sbjct: 348 VNADFVALYRCVIDGYQDTLYTHSFRQFYRECDIYGTIDYIFGNAAVVFQGCNIVSKLPM 407

Query: 319 --LDTILTAQSQSYVDERSGFTFQNCNVTVSSEISSSKDKVKVSLGRPWRNYSTVIFMES 378
               T++TAQS+   DE +G + QNC++  S ++ +S +KVK  LGRPWR +S  + MES
Sbjct: 408 PGQFTVITAQSRDTQDEDTGISMQNCSILASEDLFNSSNKVKSYLGRPWREFSRTVVMES 467

Query: 379 FVDNVVQPSGWSEWS-GTLVELLFYAEYNNSGPGADTCKRVTWPGYHVLHDEVEAANFTV 426
           ++D  +  SGWS+W+ G  ++ L+Y EYNN+GPG++T KRV WPG+H++  E +A NFT 
Sbjct: 468 YIDEFIDGSGWSKWNGGEALDTLYYGEYNNNGPGSETVKRVNWPGFHIMGYE-DAFNFTA 527

[Close]

BLAST of Sgr016281 vs. TAIR 10
Match: AT2G47550.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 214.9 bits (546), Expect = 1.3e-55
Identity = 101/247 (40.89%), Postives = 153/247 (61.94%), Query Frame = 0


Query: 198 FLTLIGDDAAATIIVDNRTV--------------KANNFMAQFVTFQNSAGPEDGQAVAV 257
           ++ +IGD    T+I  NR+V                 NF+   +T +N+AGP  GQAVA+
Sbjct: 298 YVMMIGDGINQTVITGNRSVVDGWTTFNSATFILSGPNFIGVNITIRNTAGPTKGQAVAL 357

Query: 258 LDEANHTAYYRCVFSSYQDTLYVRGMFQFFKESDIYGSVDFIFGDGLVVFQDCNIYARL- 317
               + + +Y C F +YQDTLY   + QF++E D+YG+VDFIFG+  VV Q+CN+Y R  
Sbjct: 358 RSGGDLSVFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNLYPRQP 417

Query: 318 ---LDTILTAQSQSYVDERSGFTFQNCNVTVSSEISSSKDKVKVSLGRPWRNYSTVIFME 377
                  +TAQ ++  ++ +G     C +  + ++++S   VK  LGRPW+ YS  + M+
Sbjct: 418 RKGQSNEVTAQGRTDPNQNTGTAIHGCTIRPADDLATSNYTVKTYLGRPWKEYSRTVVMQ 477

Query: 378 SFVDNVVQPSGWSEWSGTL-VELLFYAEYNNSGPGADTCKRVTWPGYHVLHDEVEAANFT 426
           +++D  ++PSGW+ WSG   +  L+YAEYNN+GPG+DT  RVTWPGYHV+ +  +A+NFT
Sbjct: 478 TYIDGFLEPSGWNAWSGDFALSTLYYAEYNNTGPGSDTTNRVTWPGYHVI-NATDASNFT 537

[Close]

BLAST of Sgr016281 vs. TAIR 10
Match: AT4G02300.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 211.5 bits (537), Expect = 1.4e-54
Identity = 103/246 (41.87%), Postives = 149/246 (60.57%), Query Frame = 0


Query: 199 LTLIGDDAAATIIVDNRT--------------VKANNFMAQFVTFQNSAGPEDGQAVAVL 258
           +  IGD    T+I  NR+              VK   ++A+ ++F NSAGP   QAVA  
Sbjct: 272 IMFIGDGIGKTVIKANRSRIDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVAFR 331

Query: 259 DEANHTAYYRCVFSSYQDTLYVRGMFQFFKESDIYGSVDFIFGDGLVVFQDCNIYARLLD 318
             ++H+A+YRC F  YQDTLYV    QF++E DIYG++DFIFG+  VVFQ+ ++YAR  +
Sbjct: 332 SGSDHSAFYRCEFDGYQDTLYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPN 391

Query: 319 ----TILTAQSQSYVDERSGFTFQNCNVTVSSEISSSKDKVKVSLGRPWRNYSTVIFMES 378
                  TAQS++  D+ +G +  NC +  + ++   K+  K  LGRPWR YS  + ++S
Sbjct: 392 PGHKIAFTAQSRNQSDQPTGISILNCRILAAPDLIPVKENFKAYLGRPWRKYSRTVIIKS 451

Query: 379 FVDNVVQPSGWSEWSGTL-VELLFYAEYNNSGPGADTCKRVTWPGYHVLHDEVEAANFTV 426
           F+D+++ P+GW E      +E L+Y EY N GPGA+  KRVTWPG+  + ++ EA  FTV
Sbjct: 452 FIDDLIHPAGWLEGKKDFALETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTV 511

The following BLAST results are available for this feature:

BLAST of Sgr016281 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022148123.1	2.6e-98	69.14	probable pectinesterase/pectinesterase inhibitor 39 [Momordica charantia]	[more]
XP_022150332.1	1.2e-87	63.04	probable pectinesterase/pectinesterase inhibitor 39 [Momordica charantia]	[more]
XP_023538248.1	1.0e-86	63.81	probable pectinesterase/pectinesterase inhibitor 39 [Cucurbita pepo subsp. pepo]	[more]
XP_022951717.1	2.3e-86	63.42	probable pectinesterase/pectinesterase inhibitor 39 [Cucurbita moschata]	[more]
KAG6585822.1	6.6e-86	63.04	putative pectinesterase/pectinesterase inhibitor 39, partial [Cucurbita argyrosp...	[more]

BLAST of Sgr016281 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8RXK7	7.9e-58	44.53	Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3...	[more]
Q9SRX4	2.3e-57	38.54	Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=37...	[more]
O48711	5.1e-57	44.31	Probable pectinesterase/pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3...	[more]
O04887	8.2e-55	41.60	Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1	[more]
O22256	1.8e-54	40.89	Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3...	[more]

BLAST of Sgr016281 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D381	1.2e-98	69.14	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111016876 PE=4 SV=1	[more]
A0A6J1DAG5	5.8e-88	63.04	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111018528 PE=4 SV=1	[more]
A0A6J1GIG0	1.1e-86	63.42	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111454459 PE=4 SV=1	[more]
A0A6J1KPG6	2.1e-85	65.57	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111496427 PE=4 SV=1	[more]
A0A6J1DCN7	6.7e-84	61.96	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111019286 PE=4 SV=1	[more]

BLAST of Sgr016281 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G02330.1	5.6e-59	44.53	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT1G02810.1	1.6e-58	38.54	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT2G26440.1	3.6e-58	44.31	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT2G47550.1	1.3e-55	40.89	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT4G02300.1	1.4e-54	41.87	Plant invertase/pectin methylesterase inhibitor superfamily	[more]

InterPro

Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 28..176 e-value: 1.8E-9 score: 47.5
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 4..169 e-value: 8.5E-16 score: 55.8
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 31..156 e-value: 1.1E-9 score: 38.8
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 28..182 e-value: 2.6E-17 score: 65.1
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 30..180
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 208..425 e-value: 3.8E-77 score: 258.9
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 191..437 e-value: 6.6E-76 score: 257.7
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 215..425
None	No IPR available	PANTHER	PTHR31707:SF188	PECTINESTERASE	coord: 215..425
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 278..287
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 197..425

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sgr016281.1	Sgr016281.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0004857	enzyme inhibitor activity
molecular_function	GO:0030599	pectinesterase activity