Sgr016278 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr016278
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
DescriptionGMC_OxRdtase_N domain-containing protein
Locationtig00007935: 625063 .. 626410 (+)
RNA-Seq ExpressionSgr016278
SyntenySgr016278
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTCTTAAAAAGTGGATATAGTGAACAAAATGACGTCAAAATAGAAGTGAATGGGGCCAATGTCTTTGTGCAGGGAAGCAACCAAGGCAGTGGGGTGATCTTTAAGGATGAAAATGGGAATCGTCATCAAGCGGTTCTTAGAAACAGGCAGCAGAGTGAGGTTATTTTATCTTCCGGAGCACTTGGGACACCTCAAATGTTGCTGCTTAGTGGAATTGGGCCAAGAGCAGATCTTGAAAAGCTGAACATATCTGTAGTGCTTGACAATCAGTTTGTCGGGAAAGGCATGGCTGATAATCCCATGAACACCGTCTTTGTTCCCACTAACAGACCTATCCAGAAATCACTTATACAGACTGTTGGGATCACTAAACTTGGTGTTTACATTGAATCTAGCAGTGGTTTTGGGCAGTCTTCGGATAGCATTCGTTGTAATCACGGGATGATGTCAGCTGAAGTAAGCTACCCTTCTTATAAGCATCAGATTCATAATTTTATCGTTTATGATATAATTGTATGTATATATATATATATATATATATGTATGTATGTATATATAGTTTTTTTTGCTAGTTATAGAATCAATTATTCATATACTGACATGTCTATTATCTATGAATGTTAATAGCTAAAATTTGTCACATACTTGAAACAAGCTATTTAATTCATGAAATCAATGATATATATGGCCATGGCGATGAAATTACTTAATGACAAGCCAGTAACTATTGTATTGGGTATATGATAATTTGTATTTCATGATGCATTGGCATTGGTGATGTAACCAGATCGGGCAACTATCCACCATTCCCCCAAAACAAAGAACCTGGGAAGCCGTCCAAGCTTACATCAAAAGGAAGCGCGATTTACCTCAGGAGGCATTCCAGGGAGGTTTCATTCTTGAAAAGATTGCCAACCCCATATCGACCGGGCAGCTTAGCTTGGCTAACACCAATGTTGATGATAATCCATCTGTGACCTTCAACTACTTCAGCCATCCCTACGATCTCCAGCGGTGTGTTGATGGCATTCGCATGGCGGCAAAAGTTGTGCAGTCAAAACACTTCAGAAAGTACACAAAAAATACTAGGCAAAGCATTGAGAAGCTGCTTAATGCGACTGTGAAGGCCAATGTCAATCTCATTCCTAAGCATACCAATGACACCAAGTCCTTAGAGCAGTTCTGCAGAGACACTGTGATAACAATTTGGCATTACCATGGAGGGTGCCATGTGGGCAAGGTAGTGAGTCCTGATTACAAGGTCCTCGGTGTTTCCCGACTCGTGTTGTTGATGGATCAACCTTTAGCGAATCGCCTGGAACCAATCCTCAAGCCACTGTCATGA

mRNA sequence

ATGTTCTTAAAAAGTGGATATAGTGAACAAAATGACGTCAAAATAGAAGTGAATGGGGCCAATGTCTTTGTGCAGGGAAGCAACCAAGGCAGTGGGGTGATCTTTAAGGATGAAAATGGGAATCGTCATCAAGCGGTTCTTAGAAACAGGCAGCAGAGTGAGGTTATTTTATCTTCCGGAGCACTTGGGACACCTCAAATGTTGCTGCTTAGTGGAATTGGGCCAAGAGCAGATCTTGAAAAGCTGAACATATCTGTAGTGCTTGACAATCAGTTTGTCGGGAAAGGCATGGCTGATAATCCCATGAACACCGTCTTTGTTCCCACTAACAGACCTATCCAGAAATCACTTATACAGACTGTTGGGATCACTAAACTTGGTGTTTACATTGAATCTAGCAGTGGTTTTGGGCAGTCTTCGGATAGCATTCGTTGTAATCACGGGATGATGTCAGCTGAAATCGGGCAACTATCCACCATTCCCCCAAAACAAAGAACCTGGGAAGCCGTCCAAGCTTACATCAAAAGGAAGCGCGATTTACCTCAGGAGGCATTCCAGGGAGGTTTCATTCTTGAAAAGATTGCCAACCCCATATCGACCGGGCAGCTTAGCTTGGCTAACACCAATGTTGATGATAATCCATCTGTGACCTTCAACTACTTCAGCCATCCCTACGATCTCCAGCGGTGTGTTGATGGCATTCGCATGGCGGCAAAAGTTGTGCAGTCAAAACACTTCAGAAAGTACACAAAAAATACTAGGCAAAGCATTGAGAAGCTGCTTAATGCGACTGTGAAGGCCAATGTCAATCTCATTCCTAAGCATACCAATGACACCAAGTCCTTAGAGCAGTTCTGCAGAGACACTGTGATAACAATTTGGCATTACCATGGAGGGTGCCATGTGGGCAAGGTAGTGAGTCCTGATTACAAGGTCCTCGGTGTTTCCCGACTCGTGTTGTTGATGGATCAACCTTTAGCGAATCGCCTGGAACCAATCCTCAAGCCACTGTCATGA

Coding sequence (CDS)

ATGTTCTTAAAAAGTGGATATAGTGAACAAAATGACGTCAAAATAGAAGTGAATGGGGCCAATGTCTTTGTGCAGGGAAGCAACCAAGGCAGTGGGGTGATCTTTAAGGATGAAAATGGGAATCGTCATCAAGCGGTTCTTAGAAACAGGCAGCAGAGTGAGGTTATTTTATCTTCCGGAGCACTTGGGACACCTCAAATGTTGCTGCTTAGTGGAATTGGGCCAAGAGCAGATCTTGAAAAGCTGAACATATCTGTAGTGCTTGACAATCAGTTTGTCGGGAAAGGCATGGCTGATAATCCCATGAACACCGTCTTTGTTCCCACTAACAGACCTATCCAGAAATCACTTATACAGACTGTTGGGATCACTAAACTTGGTGTTTACATTGAATCTAGCAGTGGTTTTGGGCAGTCTTCGGATAGCATTCGTTGTAATCACGGGATGATGTCAGCTGAAATCGGGCAACTATCCACCATTCCCCCAAAACAAAGAACCTGGGAAGCCGTCCAAGCTTACATCAAAAGGAAGCGCGATTTACCTCAGGAGGCATTCCAGGGAGGTTTCATTCTTGAAAAGATTGCCAACCCCATATCGACCGGGCAGCTTAGCTTGGCTAACACCAATGTTGATGATAATCCATCTGTGACCTTCAACTACTTCAGCCATCCCTACGATCTCCAGCGGTGTGTTGATGGCATTCGCATGGCGGCAAAAGTTGTGCAGTCAAAACACTTCAGAAAGTACACAAAAAATACTAGGCAAAGCATTGAGAAGCTGCTTAATGCGACTGTGAAGGCCAATGTCAATCTCATTCCTAAGCATACCAATGACACCAAGTCCTTAGAGCAGTTCTGCAGAGACACTGTGATAACAATTTGGCATTACCATGGAGGGTGCCATGTGGGCAAGGTAGTGAGTCCTGATTACAAGGTCCTCGGTGTTTCCCGACTCGTGTTGTTGATGGATCAACCTTTAGCGAATCGCCTGGAACCAATCCTCAAGCCACTGTCATGA

Protein sequence

MFLKSGYSEQNDVKIEVNGANVFVQGSNQGSGVIFKDENGNRHQAVLRNRQQSEVILSSGALGTPQMLLLSGIGPRADLEKLNISVVLDNQFVGKGMADNPMNTVFVPTNRPIQKSLIQTVGITKLGVYIESSSGFGQSSDSIRCNHGMMSAEIGQLSTIPPKQRTWEAVQAYIKRKRDLPQEAFQGGFILEKIANPISTGQLSLANTNVDDNPSVTFNYFSHPYDLQRCVDGIRMAAKVVQSKHFRKYTKNTRQSIEKLLNATVKANVNLIPKHTNDTKSLEQFCRDTVITIWHYHGGCHVGKVVSPDYKVLGVSRLVLLMDQPLANRLEPILKPLS
Homology
BLAST of Sgr016278 vs. NCBI nr
Match: XP_022148088.1 (protein HOTHEAD-like [Momordica charantia])

HSP 1 Score: 561.2 bits (1445), Expect = 6.0e-156
Identity = 277/287 (96.52%), Postives = 283/287 (98.61%), Query Frame = 0

Query: 32  GVIFKDENGNRHQAVLRNRQQSEVILSSGALGTPQMLLLSGIGPRADLEKLNISVVLDNQ 91
           GVIFKDENGNRHQAVLRNR QSEVILSSGA+GTPQMLLLSGIGPRADLEKLNISVVLD++
Sbjct: 268 GVIFKDENGNRHQAVLRNRGQSEVILSSGAIGTPQMLLLSGIGPRADLEKLNISVVLDSE 327

Query: 92  FVGKGMADNPMNTVFVPTNRPIQKSLIQTVGITKLGVYIESSSGFGQSSDSIRCNHGMMS 151
           FVGKGMADNPMNTVFVPTNRPIQKSLIQTVGITKLGVYIESSSGFGQSSDSIRCNHGMMS
Sbjct: 328 FVGKGMADNPMNTVFVPTNRPIQKSLIQTVGITKLGVYIESSSGFGQSSDSIRCNHGMMS 387

Query: 152 AEIGQLSTIPPKQRTWEAVQAYIKRKRDLPQEAFQGGFILEKIANPISTGQLSLANTNVD 211
           AEIGQLSTIPPKQRTWEAVQAYIKRKRDLPQEAFQGGFILEKIA+PISTGQL+LANTNVD
Sbjct: 388 AEIGQLSTIPPKQRTWEAVQAYIKRKRDLPQEAFQGGFILEKIADPISTGQLTLANTNVD 447

Query: 212 DNPSVTFNYFSHPYDLQRCVDGIRMAAKVVQSKHFRKYTKNTRQSIEKLLNATVKANVNL 271
           DNPSVTFNYF HPYDL RCVDGIRMAAKVVQSKHF KYTKNTRQSIEKLLNATVKANVNL
Sbjct: 448 DNPSVTFNYFGHPYDLHRCVDGIRMAAKVVQSKHFTKYTKNTRQSIEKLLNATVKANVNL 507

Query: 272 IPKHTNDTKSLEQFCRDTVITIWHYHGGCHVGKVVSPDYKVLGVSRL 319
           IPKHTNDTKSLEQFCRDTVI+IWHYHGGCHVGKVVSPDYKVLGVSRL
Sbjct: 508 IPKHTNDTKSLEQFCRDTVISIWHYHGGCHVGKVVSPDYKVLGVSRL 554

BLAST of Sgr016278 vs. NCBI nr
Match: XP_004146822.1 (protein HOTHEAD [Cucumis sativus] >KGN59821.1 hypothetical protein Csa_001574 [Cucumis sativus])

HSP 1 Score: 550.1 bits (1416), Expect = 1.4e-152
Identity = 269/287 (93.73%), Postives = 281/287 (97.91%), Query Frame = 0

Query: 32  GVIFKDENGNRHQAVLRNRQQSEVILSSGALGTPQMLLLSGIGPRADLEKLNISVVLDNQ 91
           GVIFKDENGNRHQAVLRNR+QSEVILSSGALG+PQMLLLSGIGPRADLEKLNISVVLDN+
Sbjct: 267 GVIFKDENGNRHQAVLRNRRQSEVILSSGALGSPQMLLLSGIGPRADLEKLNISVVLDNE 326

Query: 92  FVGKGMADNPMNTVFVPTNRPIQKSLIQTVGITKLGVYIESSSGFGQSSDSIRCNHGMMS 151
           FVGKGMADNPMNTVFVPTN+PI+KSLIQTVGITK GVYIESSSGFGQSSDSIRCNHGMMS
Sbjct: 327 FVGKGMADNPMNTVFVPTNKPIKKSLIQTVGITKFGVYIESSSGFGQSSDSIRCNHGMMS 386

Query: 152 AEIGQLSTIPPKQRTWEAVQAYIKRKRDLPQEAFQGGFILEKIANPISTGQLSLANTNVD 211
           AEIGQLSTIPPKQRTWEAVQAYI RKRDLPQEAFQGGFILEKIANP+STGQL+LANTNVD
Sbjct: 387 AEIGQLSTIPPKQRTWEAVQAYITRKRDLPQEAFQGGFILEKIANPLSTGQLTLANTNVD 446

Query: 212 DNPSVTFNYFSHPYDLQRCVDGIRMAAKVVQSKHFRKYTKNTRQSIEKLLNATVKANVNL 271
           DNPSVTFNYF+HPYDL RC+DGIR AAKVVQSKHFR+YTKNT ++IEKLLNATVKANVNL
Sbjct: 447 DNPSVTFNYFNHPYDLHRCIDGIRTAAKVVQSKHFREYTKNTGETIEKLLNATVKANVNL 506

Query: 272 IPKHTNDTKSLEQFCRDTVITIWHYHGGCHVGKVVSPDYKVLGVSRL 319
           IPKHTNDTKSLEQFCRDTVITIWHYHGGCHVGKVVSPD KVLGVSRL
Sbjct: 507 IPKHTNDTKSLEQFCRDTVITIWHYHGGCHVGKVVSPDLKVLGVSRL 553

BLAST of Sgr016278 vs. NCBI nr
Match: XP_008447607.1 (PREDICTED: protein HOTHEAD-like [Cucumis melo])

HSP 1 Score: 549.7 bits (1415), Expect = 1.8e-152
Identity = 268/287 (93.38%), Postives = 281/287 (97.91%), Query Frame = 0

Query: 32  GVIFKDENGNRHQAVLRNRQQSEVILSSGALGTPQMLLLSGIGPRADLEKLNISVVLDNQ 91
           GVIFKDENGNRHQAVLRNR+QSEVILSSGALG+PQMLLLSGIGPRADLEKLNISVVLDN+
Sbjct: 267 GVIFKDENGNRHQAVLRNRRQSEVILSSGALGSPQMLLLSGIGPRADLEKLNISVVLDNE 326

Query: 92  FVGKGMADNPMNTVFVPTNRPIQKSLIQTVGITKLGVYIESSSGFGQSSDSIRCNHGMMS 151
           FVGKGMADNPMNTVFVPTN+PI+KSLIQTVGITK GVYIESSSGFGQSSDSIRCNHGMMS
Sbjct: 327 FVGKGMADNPMNTVFVPTNKPIKKSLIQTVGITKFGVYIESSSGFGQSSDSIRCNHGMMS 386

Query: 152 AEIGQLSTIPPKQRTWEAVQAYIKRKRDLPQEAFQGGFILEKIANPISTGQLSLANTNVD 211
           AEIGQLSTIPPKQRTWEAVQAYI RKRDLPQEAFQGGFILEKIANP+STGQL+LANTNVD
Sbjct: 387 AEIGQLSTIPPKQRTWEAVQAYITRKRDLPQEAFQGGFILEKIANPLSTGQLTLANTNVD 446

Query: 212 DNPSVTFNYFSHPYDLQRCVDGIRMAAKVVQSKHFRKYTKNTRQSIEKLLNATVKANVNL 271
           DNPSVTFNYF+HPYDL RC+DGIR AAK+VQSKHFR+YTKNT ++IEKLLNATVKANVNL
Sbjct: 447 DNPSVTFNYFNHPYDLHRCIDGIRTAAKIVQSKHFREYTKNTGETIEKLLNATVKANVNL 506

Query: 272 IPKHTNDTKSLEQFCRDTVITIWHYHGGCHVGKVVSPDYKVLGVSRL 319
           IPKHTNDTKSLEQFCRDTVITIWHYHGGCHVGKVVSPD KVLGVSRL
Sbjct: 507 IPKHTNDTKSLEQFCRDTVITIWHYHGGCHVGKVVSPDLKVLGVSRL 553

BLAST of Sgr016278 vs. NCBI nr
Match: KAA0051162.1 (protein HOTHEAD-like [Cucumis melo var. makuwa] >TYK03738.1 protein HOTHEAD-like [Cucumis melo var. makuwa])

HSP 1 Score: 549.7 bits (1415), Expect = 1.8e-152
Identity = 268/287 (93.38%), Postives = 281/287 (97.91%), Query Frame = 0

Query: 32  GVIFKDENGNRHQAVLRNRQQSEVILSSGALGTPQMLLLSGIGPRADLEKLNISVVLDNQ 91
           GVIFKDENGNRHQAVLRNR+QSEVILSSGALG+PQMLLLSGIGPRADLEKLNISVVLDN+
Sbjct: 246 GVIFKDENGNRHQAVLRNRRQSEVILSSGALGSPQMLLLSGIGPRADLEKLNISVVLDNE 305

Query: 92  FVGKGMADNPMNTVFVPTNRPIQKSLIQTVGITKLGVYIESSSGFGQSSDSIRCNHGMMS 151
           FVGKGMADNPMNTVFVPTN+PI+KSLIQTVGITK GVYIESSSGFGQSSDSIRCNHGMMS
Sbjct: 306 FVGKGMADNPMNTVFVPTNKPIKKSLIQTVGITKFGVYIESSSGFGQSSDSIRCNHGMMS 365

Query: 152 AEIGQLSTIPPKQRTWEAVQAYIKRKRDLPQEAFQGGFILEKIANPISTGQLSLANTNVD 211
           AEIGQLSTIPPKQRTWEAVQAYI RKRDLPQEAFQGGFILEKIANP+STGQL+LANTNVD
Sbjct: 366 AEIGQLSTIPPKQRTWEAVQAYITRKRDLPQEAFQGGFILEKIANPLSTGQLTLANTNVD 425

Query: 212 DNPSVTFNYFSHPYDLQRCVDGIRMAAKVVQSKHFRKYTKNTRQSIEKLLNATVKANVNL 271
           DNPSVTFNYF+HPYDL RC+DGIR AAK+VQSKHFR+YTKNT ++IEKLLNATVKANVNL
Sbjct: 426 DNPSVTFNYFNHPYDLHRCIDGIRTAAKIVQSKHFREYTKNTGETIEKLLNATVKANVNL 485

Query: 272 IPKHTNDTKSLEQFCRDTVITIWHYHGGCHVGKVVSPDYKVLGVSRL 319
           IPKHTNDTKSLEQFCRDTVITIWHYHGGCHVGKVVSPD KVLGVSRL
Sbjct: 486 IPKHTNDTKSLEQFCRDTVITIWHYHGGCHVGKVVSPDLKVLGVSRL 532

BLAST of Sgr016278 vs. NCBI nr
Match: XP_038879924.1 (protein HOTHEAD [Benincasa hispida])

HSP 1 Score: 548.9 bits (1413), Expect = 3.1e-152
Identity = 269/287 (93.73%), Postives = 280/287 (97.56%), Query Frame = 0

Query: 32  GVIFKDENGNRHQAVLRNRQQSEVILSSGALGTPQMLLLSGIGPRADLEKLNISVVLDNQ 91
           GVIFKDENGNRHQAVLRNR+QSEVILSSGALG+PQMLLLSGIGPRADLEKLNISVVLDN+
Sbjct: 266 GVIFKDENGNRHQAVLRNRRQSEVILSSGALGSPQMLLLSGIGPRADLEKLNISVVLDNE 325

Query: 92  FVGKGMADNPMNTVFVPTNRPIQKSLIQTVGITKLGVYIESSSGFGQSSDSIRCNHGMMS 151
           FVGKGMADNPMNTVFVPTN+PI+KSLIQTVGITK GVYIESSSGFGQSSDSIRCNHGMMS
Sbjct: 326 FVGKGMADNPMNTVFVPTNKPIKKSLIQTVGITKFGVYIESSSGFGQSSDSIRCNHGMMS 385

Query: 152 AEIGQLSTIPPKQRTWEAVQAYIKRKRDLPQEAFQGGFILEKIANPISTGQLSLANTNVD 211
           AEIGQLSTIPPKQRTWEAVQAYI RKRDLPQEAFQGGFILEKIANPISTG+L+L NTNVD
Sbjct: 386 AEIGQLSTIPPKQRTWEAVQAYITRKRDLPQEAFQGGFILEKIANPISTGKLTLVNTNVD 445

Query: 212 DNPSVTFNYFSHPYDLQRCVDGIRMAAKVVQSKHFRKYTKNTRQSIEKLLNATVKANVNL 271
           DNPSVTFNYFSHPYDL RCVDGIRMAAK+VQSKHF  YTK+TR++IEKLLNATVKANVNL
Sbjct: 446 DNPSVTFNYFSHPYDLHRCVDGIRMAAKIVQSKHFTDYTKDTRETIEKLLNATVKANVNL 505

Query: 272 IPKHTNDTKSLEQFCRDTVITIWHYHGGCHVGKVVSPDYKVLGVSRL 319
           IPKHTNDTKSLEQFCRDTVITIWHYHGGCHVGKVVSPD KVLGVSRL
Sbjct: 506 IPKHTNDTKSLEQFCRDTVITIWHYHGGCHVGKVVSPDLKVLGVSRL 552

BLAST of Sgr016278 vs. ExPASy Swiss-Prot
Match: Q9S746 (Protein HOTHEAD OS=Arabidopsis thaliana OX=3702 GN=HTH PE=1 SV=1)

HSP 1 Score: 393.3 bits (1009), Expect = 2.8e-108
Identity = 191/288 (66.32%), Postives = 234/288 (81.25%), Query Frame = 0

Query: 31  SGVIFKDENGNRHQAVLRNRQQSEVILSSGALGTPQMLLLSGIGPRADLEKLNISVVLDN 90
           +GVIFKDE GN+HQA+L NR+ SEVILSSGA+G+PQML+LSGIGP+ +L++L I VVL+N
Sbjct: 265 TGVIFKDEKGNQHQALLSNRKGSEVILSSGAIGSPQMLMLSGIGPKKELQRLKIPVVLEN 324

Query: 91  QFVGKGMADNPMNTVFVPTNRPIQKSLIQTVGITKLGVYIESSSGFGQSSDSIRCNHGMM 150
           + VGKGMADNPMNT+ VP+  PI++SLIQTVGITK+GVY+E+S+GFGQS +SI  ++G+M
Sbjct: 325 EHVGKGMADNPMNTILVPSKAPIEQSLIQTVGITKMGVYVEASTGFGQSPESIHTHYGIM 384

Query: 151 SAEIGQLSTIPPKQRTWEAVQAYIKRKRDLPQEAFQGGFILEKIANPISTGQLSLANTNV 210
           S +    STIP KQR  EA QAYI R +    EAF G FILEK+A PIS G LSL NTNV
Sbjct: 385 SNKNELFSTIPAKQRRPEATQAYITRNKYQLHEAFNGSFILEKLAYPISRGHLSLVNTNV 444

Query: 211 DDNPSVTFNYFSHPYDLQRCVDGIRMAAKVVQSKHFRKYTKNTRQSIEKLLNATVKANVN 270
           DDNPSVTFNYF HP DLQRCV+ IR+ +KVV S  F  YT+  +Q++ K+L+ +VKAN+N
Sbjct: 445 DDNPSVTFNYFKHPVDLQRCVEAIRLVSKVVTSNRFLNYTQCDKQNVHKMLSLSVKANIN 504

Query: 271 LIPKHTNDTKSLEQFCRDTVITIWHYHGGCHVGKVVSPDYKVLGVSRL 319
           L PK  NDTKS+ QFC+DTV+TIWHYHGGC VGKVVSP+ KVLGV RL
Sbjct: 505 LRPKQLNDTKSMAQFCKDTVVTIWHYHGGCLVGKVVSPNRKVLGVDRL 552

BLAST of Sgr016278 vs. ExPASy Swiss-Prot
Match: Q9SSM2 ((R)-mandelonitrile lyase-like OS=Arabidopsis thaliana OX=3702 GN=At1g73050 PE=2 SV=1)

HSP 1 Score: 177.2 bits (448), Expect = 3.2e-43
Identity = 113/299 (37.79%), Postives = 162/299 (54.18%), Query Frame = 0

Query: 24  VQGSNQGS-GVIFKDENGNRHQAVLRNRQQSEVILSSGALGTPQMLLLSGIGPRADLEKL 83
           V GSN  + GV+++D+ G  H A++R+R   EVILS+GALG+PQ+L LSGIGPR+ L   
Sbjct: 255 VSGSNVSAIGVVYRDQLGRFHHALIRDR--GEVILSAGALGSPQLLFLSGIGPRSYLSTW 314

Query: 84  NISVVLDNQFVGKGMADNPMNTVFVPTNRPIQKSLIQTVGITKLGVYIESSSGFGQSSDS 143
            I V LD   VG  + DNP N + +    P++ SLIQ VG+T+ G ++E++S        
Sbjct: 315 GIPVALDQPHVGDFVYDNPRNGISIVPPVPMENSLIQVVGVTEDGAFLEAAS-------- 374

Query: 144 IRCNHGMMSAEIGQLSTIPPKQRTWEAVQAYIKRKRDLPQEAFQGGFILEKIANPISTGQ 203
              N    ++ +  +    P    +  V                   I+EKI  P+S G 
Sbjct: 375 ---NVIPFASPLHSVFIRAPASPLYVPVTT-----------------IMEKILGPVSIGL 434

Query: 204 LSLANTNVDDNPSVTFNYFSHPYDLQRCVDGIRMAAKVVQSKHFRKYTKNTRQSIEKLLN 263
           L LA+T+V  NP V FNYFS P DL+RCV+G R   ++++S+  + +        E   N
Sbjct: 435 LRLASTDVRINPVVRFNYFSDPQDLERCVNGTRKIGEILRSRAMQDFMIR-----EWFGN 494

Query: 264 ATVKANVNLIPKHTNDTKSLEQFCRDTVITIWHYHGGCHVGKVVSPDYKVLGVSRLVLL 322
              +     +P   ++   +  FCR TV TIWHYHGG  VGKVV  D KV+GV+ L L+
Sbjct: 495 RRFRFVGAPLPVDQSNDLVMADFCRRTVSTIWHYHGGAVVGKVVDSDLKVIGVNSLRLV 518

BLAST of Sgr016278 vs. ExPASy Swiss-Prot
Match: P52706 ((R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 169.1 bits (427), Expect = 8.7e-41
Identity = 98/289 (33.91%), Postives = 154/289 (53.29%), Query Frame = 0

Query: 31  SGVIFKDENGNRHQAVLRNRQQSEVILSSGALGTPQMLLLSGIGPRADLEKLNISVVLDN 90
           +GVI++D NG  H+A +R++   EVI+S+G +GTPQ+LLLSG+GP + L  LNI VVL +
Sbjct: 258 TGVIYRDSNGTPHRAFVRSK--GEVIVSAGTIGTPQLLLLSGVGPESYLSSLNIPVVLSH 317

Query: 91  QFVGKGMADNPMNTVFVPTNRPIQKSLIQTVGITKLGVYIESSSGFGQSSDSIRCNHGMM 150
            +VG+ + DNP N + +    PI+ +++  +GI               S+D  +C+   +
Sbjct: 318 PYVGQFLHDNPRNFINILPPNPIEPTIVTVLGI---------------SNDFYQCSFSSL 377

Query: 151 SAEIGQLSTIPPKQRTWEAVQAYIKRKRDLPQEAFQGGFILEKIANPISTGQLSL-ANTN 210
                   T PP          +      LP   F       K+A P+S G L+L +++N
Sbjct: 378 P------FTTPP-------FSFFPSTSYPLPNSTF--AHFASKVAGPLSYGSLTLKSSSN 437

Query: 211 VDDNPSVTFNYFSHPYDLQRCVDGIRMAAKVVQSKHFRKYTKNTRQSIEKLLNATVKANV 270
           V  +P+V FNY+S+P DL  CV G++   +++ +   + Y       IE      +    
Sbjct: 438 VRVSPNVKFNYYSNPTDLSHCVSGMKKIGELLSTDALKPYKVEDLPGIEGFNILGIP--- 497

Query: 271 NLIPKHTNDTKSLEQFCRDTVITIWHYHGGCHVGKVVSPDYKVLGVSRL 319
             +PK   D  + E FCR++V + WHYHGGC VGKV+  D++V G+  L
Sbjct: 498 --LPKDQTDDAAFETFCRESVASYWHYHGGCLVGKVLDGDFRVTGIDAL 509

BLAST of Sgr016278 vs. ExPASy Swiss-Prot
Match: Q945K2 ((R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1)

HSP 1 Score: 166.0 bits (419), Expect = 7.3e-40
Identity = 97/289 (33.56%), Postives = 154/289 (53.29%), Query Frame = 0

Query: 31  SGVIFKDENGNRHQAVLRNRQQSEVILSSGALGTPQMLLLSGIGPRADLEKLNISVVLDN 90
           +GVI++D NG  HQA +R++   EVI+S+G +GTPQ+LLLSG+GP + L  LNI VVL +
Sbjct: 258 TGVIYRDSNGTPHQAFVRSK--GEVIVSAGTIGTPQLLLLSGVGPESYLSSLNIPVVLSH 317

Query: 91  QFVGKGMADNPMNTVFVPTNRPIQKSLIQTVGITKLGVYIESSSGFGQSSDSIRCNHGMM 150
            +VG+ + DNP N + +    PI+ +++  +GI               S+D  +C+   +
Sbjct: 318 PYVGQFLHDNPRNFINILPPNPIEPTIVTVLGI---------------SNDFYQCSFSSL 377

Query: 151 SAEIGQLSTIPPKQRTWEAVQAYIKRKRDLPQEAFQGGFILEKIANPISTGQLSL-ANTN 210
                   T PP          +      LP   F       K+A P+S G L+L +++N
Sbjct: 378 P------FTTPP-------FGFFPSASYPLPNSTF--AHFASKVAGPLSYGSLTLKSSSN 437

Query: 211 VDDNPSVTFNYFSHPYDLQRCVDGIRMAAKVVQSKHFRKYTKNTRQSIEKLLNATVKANV 270
           V  +P+V FNY+S+  DL  CV G++   +++ +   + Y       +E      +    
Sbjct: 438 VRVSPNVKFNYYSNLTDLSHCVSGMKKIGELLSTDALKPYKVEDLPGVEGFNILGIP--- 497

Query: 271 NLIPKHTNDTKSLEQFCRDTVITIWHYHGGCHVGKVVSPDYKVLGVSRL 319
             +PK   D  + E FCR++V + WHYHGGC VGKV+  D++V G++ L
Sbjct: 498 --LPKDQTDDAAFETFCRESVASYWHYHGGCLVGKVLDGDFRVTGINAL 509

BLAST of Sgr016278 vs. ExPASy Swiss-Prot
Match: O82784 ((R)-mandelonitrile lyase 4 OS=Prunus serotina OX=23207 GN=MDL4 PE=2 SV=1)

HSP 1 Score: 163.3 bits (412), Expect = 4.8e-39
Identity = 103/318 (32.39%), Postives = 162/318 (50.94%), Query Frame = 0

Query: 9   EQNDVKIEVNGANVFVQGSNQGS-----GVIFKDENGNRHQAVLRNRQQSEVILSSGALG 68
           + +++K+ V  A   +  S + S     GV++ D NG  H+A++  +   EVILS+G LG
Sbjct: 233 DPDNLKVAVEAAVQKIIFSTESSGLTAVGVVYTDSNGTSHRALVSGK--GEVILSAGTLG 292

Query: 69  TPQMLLLSGIGPRADLEKLNISVVLDNQFVGKGMADNPMNTVFVPTNRPIQKSLIQTVGI 128
           TPQ+LLLSG+GP + L  LNISVV  + +VG+ + DNP N + +    PI+ S +  +GI
Sbjct: 293 TPQLLLLSGVGPESYLTSLNISVVASHPYVGQYVNDNPRNFINILPPNPIEPSTVTVLGI 352

Query: 129 TKLGVYIESSSGFGQSSDSIRCNHGMMSAEIGQLSTIPPKQRTWEAVQAYIKRKRDLPQE 188
           T               SD  +C+   +  +    S  P                  LP +
Sbjct: 353 T---------------SDFYQCSLSSLPFDTPPFSLFP-------------TTSYPLPNQ 412

Query: 189 AFQGGFILEKIANPISTGQLSL-ANTNVDDNPSVTFNYFSHPYDLQRCVDGIRMAAKVVQ 248
            F    I+ K+  P+S G L+L +++NV   P+V FNY S P DL  CV G++     + 
Sbjct: 413 TF--AHIVSKVPGPLSAGSLTLQSSSNVSVAPNVKFNYCSDPVDLTHCVSGMKKIGVFLS 472

Query: 249 SKHFRKYTKNTRQSIE--KLLNATVKANVNLIPKHTNDTKSLEQFCRDTVITIWHYHGGC 308
           +   + Y  +    I+   +L          +P++  D  + E+FCRDTV + WHYHGG 
Sbjct: 473 TDALKPYKVDDLPGIDGFNILGTP-------LPENQTDDAAFEKFCRDTVASYWHYHGGA 511

Query: 309 HVGKVVSPDYKVLGVSRL 319
            VGKV+  +++V G++ L
Sbjct: 533 IVGKVIDGNFRVTGINAL 511

BLAST of Sgr016278 vs. ExPASy TrEMBL
Match: A0A6J1D343 (protein HOTHEAD-like OS=Momordica charantia OX=3673 GN=LOC111016853 PE=3 SV=1)

HSP 1 Score: 561.2 bits (1445), Expect = 2.9e-156
Identity = 277/287 (96.52%), Postives = 283/287 (98.61%), Query Frame = 0

Query: 32  GVIFKDENGNRHQAVLRNRQQSEVILSSGALGTPQMLLLSGIGPRADLEKLNISVVLDNQ 91
           GVIFKDENGNRHQAVLRNR QSEVILSSGA+GTPQMLLLSGIGPRADLEKLNISVVLD++
Sbjct: 268 GVIFKDENGNRHQAVLRNRGQSEVILSSGAIGTPQMLLLSGIGPRADLEKLNISVVLDSE 327

Query: 92  FVGKGMADNPMNTVFVPTNRPIQKSLIQTVGITKLGVYIESSSGFGQSSDSIRCNHGMMS 151
           FVGKGMADNPMNTVFVPTNRPIQKSLIQTVGITKLGVYIESSSGFGQSSDSIRCNHGMMS
Sbjct: 328 FVGKGMADNPMNTVFVPTNRPIQKSLIQTVGITKLGVYIESSSGFGQSSDSIRCNHGMMS 387

Query: 152 AEIGQLSTIPPKQRTWEAVQAYIKRKRDLPQEAFQGGFILEKIANPISTGQLSLANTNVD 211
           AEIGQLSTIPPKQRTWEAVQAYIKRKRDLPQEAFQGGFILEKIA+PISTGQL+LANTNVD
Sbjct: 388 AEIGQLSTIPPKQRTWEAVQAYIKRKRDLPQEAFQGGFILEKIADPISTGQLTLANTNVD 447

Query: 212 DNPSVTFNYFSHPYDLQRCVDGIRMAAKVVQSKHFRKYTKNTRQSIEKLLNATVKANVNL 271
           DNPSVTFNYF HPYDL RCVDGIRMAAKVVQSKHF KYTKNTRQSIEKLLNATVKANVNL
Sbjct: 448 DNPSVTFNYFGHPYDLHRCVDGIRMAAKVVQSKHFTKYTKNTRQSIEKLLNATVKANVNL 507

Query: 272 IPKHTNDTKSLEQFCRDTVITIWHYHGGCHVGKVVSPDYKVLGVSRL 319
           IPKHTNDTKSLEQFCRDTVI+IWHYHGGCHVGKVVSPDYKVLGVSRL
Sbjct: 508 IPKHTNDTKSLEQFCRDTVISIWHYHGGCHVGKVVSPDYKVLGVSRL 554

BLAST of Sgr016278 vs. ExPASy TrEMBL
Match: A0A0A0LIE5 (GMC_OxRdtase_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G848300 PE=3 SV=1)

HSP 1 Score: 550.1 bits (1416), Expect = 6.7e-153
Identity = 269/287 (93.73%), Postives = 281/287 (97.91%), Query Frame = 0

Query: 32  GVIFKDENGNRHQAVLRNRQQSEVILSSGALGTPQMLLLSGIGPRADLEKLNISVVLDNQ 91
           GVIFKDENGNRHQAVLRNR+QSEVILSSGALG+PQMLLLSGIGPRADLEKLNISVVLDN+
Sbjct: 267 GVIFKDENGNRHQAVLRNRRQSEVILSSGALGSPQMLLLSGIGPRADLEKLNISVVLDNE 326

Query: 92  FVGKGMADNPMNTVFVPTNRPIQKSLIQTVGITKLGVYIESSSGFGQSSDSIRCNHGMMS 151
           FVGKGMADNPMNTVFVPTN+PI+KSLIQTVGITK GVYIESSSGFGQSSDSIRCNHGMMS
Sbjct: 327 FVGKGMADNPMNTVFVPTNKPIKKSLIQTVGITKFGVYIESSSGFGQSSDSIRCNHGMMS 386

Query: 152 AEIGQLSTIPPKQRTWEAVQAYIKRKRDLPQEAFQGGFILEKIANPISTGQLSLANTNVD 211
           AEIGQLSTIPPKQRTWEAVQAYI RKRDLPQEAFQGGFILEKIANP+STGQL+LANTNVD
Sbjct: 387 AEIGQLSTIPPKQRTWEAVQAYITRKRDLPQEAFQGGFILEKIANPLSTGQLTLANTNVD 446

Query: 212 DNPSVTFNYFSHPYDLQRCVDGIRMAAKVVQSKHFRKYTKNTRQSIEKLLNATVKANVNL 271
           DNPSVTFNYF+HPYDL RC+DGIR AAKVVQSKHFR+YTKNT ++IEKLLNATVKANVNL
Sbjct: 447 DNPSVTFNYFNHPYDLHRCIDGIRTAAKVVQSKHFREYTKNTGETIEKLLNATVKANVNL 506

Query: 272 IPKHTNDTKSLEQFCRDTVITIWHYHGGCHVGKVVSPDYKVLGVSRL 319
           IPKHTNDTKSLEQFCRDTVITIWHYHGGCHVGKVVSPD KVLGVSRL
Sbjct: 507 IPKHTNDTKSLEQFCRDTVITIWHYHGGCHVGKVVSPDLKVLGVSRL 553

BLAST of Sgr016278 vs. ExPASy TrEMBL
Match: A0A5A7U5M2 (Protein HOTHEAD-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold863G001160 PE=3 SV=1)

HSP 1 Score: 549.7 bits (1415), Expect = 8.7e-153
Identity = 268/287 (93.38%), Postives = 281/287 (97.91%), Query Frame = 0

Query: 32  GVIFKDENGNRHQAVLRNRQQSEVILSSGALGTPQMLLLSGIGPRADLEKLNISVVLDNQ 91
           GVIFKDENGNRHQAVLRNR+QSEVILSSGALG+PQMLLLSGIGPRADLEKLNISVVLDN+
Sbjct: 246 GVIFKDENGNRHQAVLRNRRQSEVILSSGALGSPQMLLLSGIGPRADLEKLNISVVLDNE 305

Query: 92  FVGKGMADNPMNTVFVPTNRPIQKSLIQTVGITKLGVYIESSSGFGQSSDSIRCNHGMMS 151
           FVGKGMADNPMNTVFVPTN+PI+KSLIQTVGITK GVYIESSSGFGQSSDSIRCNHGMMS
Sbjct: 306 FVGKGMADNPMNTVFVPTNKPIKKSLIQTVGITKFGVYIESSSGFGQSSDSIRCNHGMMS 365

Query: 152 AEIGQLSTIPPKQRTWEAVQAYIKRKRDLPQEAFQGGFILEKIANPISTGQLSLANTNVD 211
           AEIGQLSTIPPKQRTWEAVQAYI RKRDLPQEAFQGGFILEKIANP+STGQL+LANTNVD
Sbjct: 366 AEIGQLSTIPPKQRTWEAVQAYITRKRDLPQEAFQGGFILEKIANPLSTGQLTLANTNVD 425

Query: 212 DNPSVTFNYFSHPYDLQRCVDGIRMAAKVVQSKHFRKYTKNTRQSIEKLLNATVKANVNL 271
           DNPSVTFNYF+HPYDL RC+DGIR AAK+VQSKHFR+YTKNT ++IEKLLNATVKANVNL
Sbjct: 426 DNPSVTFNYFNHPYDLHRCIDGIRTAAKIVQSKHFREYTKNTGETIEKLLNATVKANVNL 485

Query: 272 IPKHTNDTKSLEQFCRDTVITIWHYHGGCHVGKVVSPDYKVLGVSRL 319
           IPKHTNDTKSLEQFCRDTVITIWHYHGGCHVGKVVSPD KVLGVSRL
Sbjct: 486 IPKHTNDTKSLEQFCRDTVITIWHYHGGCHVGKVVSPDLKVLGVSRL 532

BLAST of Sgr016278 vs. ExPASy TrEMBL
Match: A0A1S3BH95 (protein HOTHEAD-like OS=Cucumis melo OX=3656 GN=LOC103490022 PE=3 SV=1)

HSP 1 Score: 549.7 bits (1415), Expect = 8.7e-153
Identity = 268/287 (93.38%), Postives = 281/287 (97.91%), Query Frame = 0

Query: 32  GVIFKDENGNRHQAVLRNRQQSEVILSSGALGTPQMLLLSGIGPRADLEKLNISVVLDNQ 91
           GVIFKDENGNRHQAVLRNR+QSEVILSSGALG+PQMLLLSGIGPRADLEKLNISVVLDN+
Sbjct: 267 GVIFKDENGNRHQAVLRNRRQSEVILSSGALGSPQMLLLSGIGPRADLEKLNISVVLDNE 326

Query: 92  FVGKGMADNPMNTVFVPTNRPIQKSLIQTVGITKLGVYIESSSGFGQSSDSIRCNHGMMS 151
           FVGKGMADNPMNTVFVPTN+PI+KSLIQTVGITK GVYIESSSGFGQSSDSIRCNHGMMS
Sbjct: 327 FVGKGMADNPMNTVFVPTNKPIKKSLIQTVGITKFGVYIESSSGFGQSSDSIRCNHGMMS 386

Query: 152 AEIGQLSTIPPKQRTWEAVQAYIKRKRDLPQEAFQGGFILEKIANPISTGQLSLANTNVD 211
           AEIGQLSTIPPKQRTWEAVQAYI RKRDLPQEAFQGGFILEKIANP+STGQL+LANTNVD
Sbjct: 387 AEIGQLSTIPPKQRTWEAVQAYITRKRDLPQEAFQGGFILEKIANPLSTGQLTLANTNVD 446

Query: 212 DNPSVTFNYFSHPYDLQRCVDGIRMAAKVVQSKHFRKYTKNTRQSIEKLLNATVKANVNL 271
           DNPSVTFNYF+HPYDL RC+DGIR AAK+VQSKHFR+YTKNT ++IEKLLNATVKANVNL
Sbjct: 447 DNPSVTFNYFNHPYDLHRCIDGIRTAAKIVQSKHFREYTKNTGETIEKLLNATVKANVNL 506

Query: 272 IPKHTNDTKSLEQFCRDTVITIWHYHGGCHVGKVVSPDYKVLGVSRL 319
           IPKHTNDTKSLEQFCRDTVITIWHYHGGCHVGKVVSPD KVLGVSRL
Sbjct: 507 IPKHTNDTKSLEQFCRDTVITIWHYHGGCHVGKVVSPDLKVLGVSRL 553

BLAST of Sgr016278 vs. ExPASy TrEMBL
Match: A0A6J1GTL1 (protein HOTHEAD-like OS=Cucurbita moschata OX=3662 GN=LOC111457419 PE=3 SV=1)

HSP 1 Score: 545.0 bits (1403), Expect = 2.1e-151
Identity = 266/287 (92.68%), Postives = 280/287 (97.56%), Query Frame = 0

Query: 32  GVIFKDENGNRHQAVLRNRQQSEVILSSGALGTPQMLLLSGIGPRADLEKLNISVVLDNQ 91
           GVIFKDENGNRH+AVLRNR+QSEVILSSGALG+PQMLLLSGIGPRADLEKLNISVVLDN+
Sbjct: 269 GVIFKDENGNRHEAVLRNRRQSEVILSSGALGSPQMLLLSGIGPRADLEKLNISVVLDNE 328

Query: 92  FVGKGMADNPMNTVFVPTNRPIQKSLIQTVGITKLGVYIESSSGFGQSSDSIRCNHGMMS 151
           FVGKGMADNPMNTVFVPTNRPI+KSLIQTVGITK GVYIESSSGFGQSSDSIRCNHGMMS
Sbjct: 329 FVGKGMADNPMNTVFVPTNRPIKKSLIQTVGITKFGVYIESSSGFGQSSDSIRCNHGMMS 388

Query: 152 AEIGQLSTIPPKQRTWEAVQAYIKRKRDLPQEAFQGGFILEKIANPISTGQLSLANTNVD 211
           AEIGQLSTIPPKQRTWEA QAYIK KRDLPQEAF GGFILEKIA+P+STGQL+LANTNVD
Sbjct: 389 AEIGQLSTIPPKQRTWEAAQAYIKTKRDLPQEAFWGGFILEKIADPLSTGQLTLANTNVD 448

Query: 212 DNPSVTFNYFSHPYDLQRCVDGIRMAAKVVQSKHFRKYTKNTRQSIEKLLNATVKANVNL 271
           DNP+VTFNYF+HPYDL RCVDGIRMAAKVVQSKHF  +TKNTR++IEKLLNATVKANVNL
Sbjct: 449 DNPTVTFNYFNHPYDLHRCVDGIRMAAKVVQSKHFTAFTKNTRETIEKLLNATVKANVNL 508

Query: 272 IPKHTNDTKSLEQFCRDTVITIWHYHGGCHVGKVVSPDYKVLGVSRL 319
           IPKHTNDTKSLEQFCRDTVI+IWHYHGGCHVGKVVSPDYKVLGVSRL
Sbjct: 509 IPKHTNDTKSLEQFCRDTVISIWHYHGGCHVGKVVSPDYKVLGVSRL 555

BLAST of Sgr016278 vs. TAIR 10
Match: AT1G72970.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 393.3 bits (1009), Expect = 2.0e-109
Identity = 191/288 (66.32%), Postives = 234/288 (81.25%), Query Frame = 0

Query: 31  SGVIFKDENGNRHQAVLRNRQQSEVILSSGALGTPQMLLLSGIGPRADLEKLNISVVLDN 90
           +GVIFKDE GN+HQA+L NR+ SEVILSSGA+G+PQML+LSGIGP+ +L++L I VVL+N
Sbjct: 265 TGVIFKDEKGNQHQALLSNRKGSEVILSSGAIGSPQMLMLSGIGPKKELQRLKIPVVLEN 324

Query: 91  QFVGKGMADNPMNTVFVPTNRPIQKSLIQTVGITKLGVYIESSSGFGQSSDSIRCNHGMM 150
           + VGKGMADNPMNT+ VP+  PI++SLIQTVGITK+GVY+E+S+GFGQS +SI  ++G+M
Sbjct: 325 EHVGKGMADNPMNTILVPSKAPIEQSLIQTVGITKMGVYVEASTGFGQSPESIHTHYGIM 384

Query: 151 SAEIGQLSTIPPKQRTWEAVQAYIKRKRDLPQEAFQGGFILEKIANPISTGQLSLANTNV 210
           S +    STIP KQR  EA QAYI R +    EAF G FILEK+A PIS G LSL NTNV
Sbjct: 385 SNKNELFSTIPAKQRRPEATQAYITRNKYQLHEAFNGSFILEKLAYPISRGHLSLVNTNV 444

Query: 211 DDNPSVTFNYFSHPYDLQRCVDGIRMAAKVVQSKHFRKYTKNTRQSIEKLLNATVKANVN 270
           DDNPSVTFNYF HP DLQRCV+ IR+ +KVV S  F  YT+  +Q++ K+L+ +VKAN+N
Sbjct: 445 DDNPSVTFNYFKHPVDLQRCVEAIRLVSKVVTSNRFLNYTQCDKQNVHKMLSLSVKANIN 504

Query: 271 LIPKHTNDTKSLEQFCRDTVITIWHYHGGCHVGKVVSPDYKVLGVSRL 319
           L PK  NDTKS+ QFC+DTV+TIWHYHGGC VGKVVSP+ KVLGV RL
Sbjct: 505 LRPKQLNDTKSMAQFCKDTVVTIWHYHGGCLVGKVVSPNRKVLGVDRL 552

BLAST of Sgr016278 vs. TAIR 10
Match: AT1G72970.2 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 350.9 bits (899), Expect = 1.1e-96
Identity = 177/288 (61.46%), Postives = 216/288 (75.00%), Query Frame = 0

Query: 31  SGVIFKDENGNRHQAVLRNRQQSEVILSSGALGTPQMLLLSGIGPRADLEKLNISVVLDN 90
           +GVIFKDE GN+HQA+L NR+ SEVILSSGA+G+PQML+LSGIGP+ +L++L I VVL+N
Sbjct: 265 TGVIFKDEKGNQHQALLSNRKGSEVILSSGAIGSPQMLMLSGIGPKKELQRLKIPVVLEN 324

Query: 91  QFVGKGMADNPMNTVFVPTNRPIQKSLIQTVGITKLGVYIESSSGFGQSSDSIRCNHGMM 150
           + VGKGMADNPMNT+ VP+  PI++SLIQTVGITK+GVY+E+S+GFGQS +SI  ++G+M
Sbjct: 325 EHVGKGMADNPMNTILVPSKAPIEQSLIQTVGITKMGVYVEASTGFGQSPESIHTHYGIM 384

Query: 151 SAEIGQLSTIPPKQRTWEAVQAYIKRKRDLPQEAFQGGFILEKIANPISTGQLSLANTNV 210
           S +    STIP KQR  EA QAYI R +    EAF G FILEK+A PIS G LSL NTNV
Sbjct: 385 SNKNELFSTIPAKQRRPEATQAYITRNKYQLHEAFNGSFILEKLAYPISRGHLSLVNTNV 444

Query: 211 DDNPSVTFNYFSHPYDLQRCVDGIRMAAKVVQSKHFRKYTKNTRQSIEKLLNATVKANVN 270
           DDNPSVTFNYF HP D                           +Q++ K+L+ +VKAN+N
Sbjct: 445 DDNPSVTFNYFKHPCD---------------------------KQNVHKMLSLSVKANIN 504

Query: 271 LIPKHTNDTKSLEQFCRDTVITIWHYHGGCHVGKVVSPDYKVLGVSRL 319
           L PK  NDTKS+ QFC+DTV+TIWHYHGGC VGKVVSP+ KVLGV RL
Sbjct: 505 LRPKQLNDTKSMAQFCKDTVVTIWHYHGGCLVGKVVSPNRKVLGVDRL 525

BLAST of Sgr016278 vs. TAIR 10
Match: AT1G12570.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 255.0 bits (650), Expect = 8.5e-68
Identity = 144/300 (48.00%), Postives = 184/300 (61.33%), Query Frame = 0

Query: 31  SGVIFKDENGNRHQAVLRNRQQSEVILSSGALGTPQMLLLSGIGPRADLEKLNISVVLDN 90
           +GV+++D  G  H+A L+    SE+ILS+G LG+PQ+L+LSG+GP A L+  NI+VV+D 
Sbjct: 249 NGVVYRDRTGQAHRAYLKEGALSEIILSAGTLGSPQLLMLSGVGPSAQLQAQNITVVMDQ 308

Query: 91  QFVGKGMADNPMNTVFVPTNRPIQKSLIQTVGITKLGVYIESSSG--FG------QSSDS 150
             VG+GM DNPMN VFVP+  P++ SLI+ VGIT  G Y+E++ G  FG        S S
Sbjct: 309 PHVGQGMYDNPMNAVFVPSPVPVEVSLIEVVGITGEGTYVEAAGGENFGGGGGGSSGSSS 368

Query: 151 IRCNHGMMSAEIGQLSTIPPKQRTWEAVQAYIKRKRDLPQEAFQGGFILEKIANPISTGQ 210
            R  + M S          P+    E+  +  K     P   FQGGF+LEK+  P+STG 
Sbjct: 369 TRDYYAMFS----------PRATLLES-NSMTKLSSAQP---FQGGFLLEKVMGPLSTGH 428

Query: 211 LSLANTNVDDNPSVTFNYFSHPYDLQRCVDGIRMAAKVVQSKHFRKYTKNTRQSIEKLLN 270
           L L   N  DNP VTFNYF HP DL+RCV GI+   +VVQSK F +Y K    S E LLN
Sbjct: 429 LELKTRNPKDNPIVTFNYFQHPDDLKRCVRGIQTIERVVQSKAFSRY-KYADVSFEYLLN 488

Query: 271 ATVKANVNLIPKHTNDTKSL----EQFCRDTVITIWHYHGGCHVGKVVSPDYKVLGVSRL 319
            T    VNL P  +    SL    E+FC+ TV TIWHYHGGC VG+VV  DYKV+G+ RL
Sbjct: 489 LTASTPVNLRPPRSGPGASLPPSAEEFCQHTVTTIWHYHGGCVVGRVVDGDYKVIGIDRL 533

BLAST of Sgr016278 vs. TAIR 10
Match: AT5G51950.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 245.7 bits (626), Expect = 5.2e-65
Identity = 138/294 (46.94%), Postives = 176/294 (59.86%), Query Frame = 0

Query: 32  GVIFKDENGNRHQAVLRNRQQSEVILSSGALGTPQMLLLSGIGPRADLEKLNIS-VVLDN 91
           GVIF+D NG  H+A L     +EVILS+GA+G+PQ+L+LSGIGP A L    I  +VLD+
Sbjct: 250 GVIFQDANGVLHKAELEKNSMNEVILSAGAIGSPQLLMLSGIGPAAHLAAHGIKPLVLDH 309

Query: 92  QFVGKGMADNPMNTVFVPTNRPIQKSLIQTVGITKLGVYIESSSGFGQSSDSIR------ 151
             VG+GM DNPMN +F+P+  P++ SLIQ VGITK   YIE +SG   S    R      
Sbjct: 310 PMVGQGMGDNPMNAIFIPSPTPVEVSLIQVVGITKFESYIEGASGVIFSYSWTRRFFDGV 369

Query: 152 CNHGMMSAEIGQLSTIPPKQRTWEAVQAYIKRKRDLPQEAFQGGFILEKIANPISTGQLS 211
            N+          ST  P   T +++  +      L     + G IL+KIA PIS G L 
Sbjct: 370 LNYLNEMQTSRTTSTTSPTLST-QSITDFFNPINPLLNATTRAGLILQKIAGPISRGHLE 429

Query: 212 LANTNVDDNPSVTFNYFSHPYDLQRCVDGIRMAAKVVQSKHFRKYTKNTRQSIEKLLNAT 271
           L NTN DDNPSV FNY+  P DLQ CV+GI    KV+ SK F K+ K    +I  LL+  
Sbjct: 430 LRNTNPDDNPSVRFNYYQEPEDLQICVEGINTIIKVINSKAFSKF-KYPDATIHGLLDLM 489

Query: 272 VKANVNLIPKHTNDTKSLEQFCRDTVITIWHYHGGCHVGKVVSPDYKVLGVSRL 319
           +    NL P+H     +L QFC DTV+TIWHYHGGC VG+VV  +Y+VLG+  L
Sbjct: 490 LSVPTNLRPRHITSMFNLRQFCIDTVMTIWHYHGGCQVGRVVDKNYRVLGIDSL 541

BLAST of Sgr016278 vs. TAIR 10
Match: AT5G51950.2 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 245.7 bits (626), Expect = 5.2e-65
Identity = 138/294 (46.94%), Postives = 176/294 (59.86%), Query Frame = 0

Query: 32  GVIFKDENGNRHQAVLRNRQQSEVILSSGALGTPQMLLLSGIGPRADLEKLNIS-VVLDN 91
           GVIF+D NG  H+A L     +EVILS+GA+G+PQ+L+LSGIGP A L    I  +VLD+
Sbjct: 217 GVIFQDANGVLHKAELEKNSMNEVILSAGAIGSPQLLMLSGIGPAAHLAAHGIKPLVLDH 276

Query: 92  QFVGKGMADNPMNTVFVPTNRPIQKSLIQTVGITKLGVYIESSSGFGQSSDSIR------ 151
             VG+GM DNPMN +F+P+  P++ SLIQ VGITK   YIE +SG   S    R      
Sbjct: 277 PMVGQGMGDNPMNAIFIPSPTPVEVSLIQVVGITKFESYIEGASGVIFSYSWTRRFFDGV 336

Query: 152 CNHGMMSAEIGQLSTIPPKQRTWEAVQAYIKRKRDLPQEAFQGGFILEKIANPISTGQLS 211
            N+          ST  P   T +++  +      L     + G IL+KIA PIS G L 
Sbjct: 337 LNYLNEMQTSRTTSTTSPTLST-QSITDFFNPINPLLNATTRAGLILQKIAGPISRGHLE 396

Query: 212 LANTNVDDNPSVTFNYFSHPYDLQRCVDGIRMAAKVVQSKHFRKYTKNTRQSIEKLLNAT 271
           L NTN DDNPSV FNY+  P DLQ CV+GI    KV+ SK F K+ K    +I  LL+  
Sbjct: 397 LRNTNPDDNPSVRFNYYQEPEDLQICVEGINTIIKVINSKAFSKF-KYPDATIHGLLDLM 456

Query: 272 VKANVNLIPKHTNDTKSLEQFCRDTVITIWHYHGGCHVGKVVSPDYKVLGVSRL 319
           +    NL P+H     +L QFC DTV+TIWHYHGGC VG+VV  +Y+VLG+  L
Sbjct: 457 LSVPTNLRPRHITSMFNLRQFCIDTVMTIWHYHGGCQVGRVVDKNYRVLGIDSL 508

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022148088.16.0e-15696.52protein HOTHEAD-like [Momordica charantia][more]
XP_004146822.11.4e-15293.73protein HOTHEAD [Cucumis sativus] >KGN59821.1 hypothetical protein Csa_001574 [C... [more]
XP_008447607.11.8e-15293.38PREDICTED: protein HOTHEAD-like [Cucumis melo][more]
KAA0051162.11.8e-15293.38protein HOTHEAD-like [Cucumis melo var. makuwa] >TYK03738.1 protein HOTHEAD-like... [more]
XP_038879924.13.1e-15293.73protein HOTHEAD [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q9S7462.8e-10866.32Protein HOTHEAD OS=Arabidopsis thaliana OX=3702 GN=HTH PE=1 SV=1[more]
Q9SSM23.2e-4337.79(R)-mandelonitrile lyase-like OS=Arabidopsis thaliana OX=3702 GN=At1g73050 PE=2 ... [more]
P527068.7e-4133.91(R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1[more]
Q945K27.3e-4033.56(R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1[more]
O827844.8e-3932.39(R)-mandelonitrile lyase 4 OS=Prunus serotina OX=23207 GN=MDL4 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1D3432.9e-15696.52protein HOTHEAD-like OS=Momordica charantia OX=3673 GN=LOC111016853 PE=3 SV=1[more]
A0A0A0LIE56.7e-15393.73GMC_OxRdtase_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G848... [more]
A0A5A7U5M28.7e-15393.38Protein HOTHEAD-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold863... [more]
A0A1S3BH958.7e-15393.38protein HOTHEAD-like OS=Cucumis melo OX=3656 GN=LOC103490022 PE=3 SV=1[more]
A0A6J1GTL12.1e-15192.68protein HOTHEAD-like OS=Cucurbita moschata OX=3662 GN=LOC111457419 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G72970.12.0e-10966.32Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT1G72970.21.1e-9661.46Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT1G12570.18.5e-6848.00Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT5G51950.15.2e-6546.94Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT5G51950.25.2e-6546.94Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036188FAD/NAD(P)-binding domain superfamilyGENE3D3.50.50.60coord: 27..318
e-value: 7.0E-72
score: 244.9
IPR036188FAD/NAD(P)-binding domain superfamilySUPERFAMILY51905FAD/NAD(P)-binding domaincoord: 31..121
NoneNo IPR availableGENE3D3.30.410.40coord: 100..292
e-value: 7.0E-72
score: 244.9
NoneNo IPR availablePANTHERPTHR45968OSJNBA0019K04.7 PROTEINcoord: 28..321
NoneNo IPR availableSUPERFAMILY54373FAD-linked reductases, C-terminal domaincoord: 97..299
IPR007867Glucose-methanol-choline oxidoreductase, C-terminalPFAMPF05199GMC_oxred_Ccoord: 197..319
e-value: 8.2E-18
score: 65.3
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPFAMPF00732GMC_oxred_Ncoord: 26..101
e-value: 5.9E-13
score: 48.8
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPROSITEPS00624GMC_OXRED_2coord: 60..74
IPR031206Protein Hothead-likePANTHERPTHR45968:SF5PROTEIN HOTHEADcoord: 28..321

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr016278.1Sgr016278.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0016614 oxidoreductase activity, acting on CH-OH group of donors