Sgr016275 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr016275
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
DescriptionPhospholipase D
Locationtig00007935: 569724 .. 572457 (+)
RNA-Seq ExpressionSgr016275
SyntenySgr016275
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGGGAAAGCAGAAGTTCTTCCACGGAACGCTCGAAGTTACCATCTTCCATGCAACAGCATACACACCGCCGTCACCTCTCGATGTAAGTTCTGGATCATAAGAATGCAAACACATAATTTCAGAGACATTTCTATACCTTCAGTAGAAAAAAGTTTATCAAGTAATAACATAGAGAGTGATCAAAACATTGGCGCGGTCTTACCTTTGTTTTGCTCTCTTTTGCAGTGCATATTTGCAGGAGGAAAGCGTAGCTATGTGACCGTCAAGATAGACAACAAGAGGTTGCCCAGACGAGTCACGAGTACGACCGTGTTTGGAACCAGACATTCCAAGTTCTTTGCGCACATCCATTGACTTCTACCATCACCATTACAATGAAAACATCTCGTTCTGTCTTGGGTAAATTCCACATCCCAGCCCAACAGATTTTGAACGAAGCAAGTTTCATCAATGGTTTCTTTCCGCTTCTTATGGAAAATGGGAAGCCTAGCCCGGAGCTCAGACTTCGATTCATGCTGTGGTTTAAACCGGCAGTATATGAATTAAGCTGGAAAAAGATGCTGGGAAATGGAGAATACAAGGGGCTGAGGAATGCAACATTTCATCTAAGGTCCAATTGTCATGTAACTCTATATCAAGATGCTCACCACCTCCCTACTTTTCGACCTCCGTTTCACGGTTCGAGTGCACCAAGAAGGCTATGGGAAGATATATACAAGGCCATAGATAATGCAAAGCATTTGATTTATATTGCAGGCTGGTCCTTCAATCCAAAGATGGTGCTGGTAAATCGAAGCAATCACCTATCTTTGTAATCAATAATGTCCTTTTCTTTCTTTCTCTTTTTTGTGGTTGTAAACAGCTAAAACGCTTCTGACCAGGTAAGAGACTCTCAGACTGATATACCCTATGCTTTAGGAGTAAAGCTTGGTGAGCTGTTGAAGCAGAAGGCAGAGGAAGGTGTAGCTGTGAGAATATTGATTTGGGACGATGAAACATCCCTTCCCATCATCAAGAATGCCGGGATAATGAAAACACGCGATGAAGATGCTCGGGCATATTTCTCGCACTCAAAAGTTATATGCAAGTTGTGTCCCAAATTACACCCTATGTCCCCACCAATTTTCTCTCACCATCAGAAAACCATAGTTGTAGACGCTCAAACACACATCAATGCACAAAACAGGGAGATTATGAGTTTCATTGGGGGTTTAGATCTTTGTGATGGTCGCTATGACACTGATCAACATTCATTGTTCCATACTTTGAATATGGAATCCCACTGCTGTGATTTCTATCAGACAAGTATATCAGGTGCCAAGCTTCAAAAAGGAGGGCCAAGAGAGCCATGGCATGATGTTCATGCTTGTGTAACAGGTGAAGCTGCTTGGGATATATTAACAAACTTTGAGCAACGATGGACTAAGCAATCAGATGCTTCATTGTTAGTTCCCACAAACATCTTATTAAATCTGATGCCCCAGCTCGAAACAAACACTAACCCACAAAAGGATTGGAACGTGCAAGTTTTTCGATCTATTGACCACTTGTCTGCCAGCCAAGTATTCAGAAACTTGACAGTTGAACGAACCATCCATGAAGCTTATGTTGAAGCCATCAGGCGAGCGGATAGATTTATTTACATCGAAAACCAATATTTCATAGGAGGGTGCCACTTGTGGGACAAAGATCAACACTGCGGATGCACGAATTTGATACCGATTGAGATTGCACTCAAGGTGGCTAATAAGATAAAGGCAAGGGAGAGGTTTGCAGTTTATATAGTGATACCAATGTGGCCAGAAGGACCACCAGAGAGTGAATCAGTTGAGGATATGCTACATTGGACTAGGCAGACAATAACAATGATGTATAGACTGATTGGAGAAGCCATCCAAGAAACTGGGGAGATGGCCCACCCAAGAGACTACTTGAATTTCTTTTGCCTTGCAAACAGAGAAGAAGAGAGAAAATGGGACTTTGTTCCCCCACACAGTCCCCAACAGGCAACACAGTACTGGAATGCCCAACAACATAGGAGATTCATGGTCTATGTCCATTCCAAGGTCATGATAGGTAAGACTAAGTGTTCTGAATCTCATTTCTAAGGGGCATTTTAACTAACATGTAGAGAGCATTTTAACAAACATAAACATCAAACAAATGTCGAATTGATAATCCATAAATTGATGTGGGGCTCATTAATTATTGAAATTGCACTTATTTGAAGCAAAAATGTTGAAGAAATAGAAACTAACCATGAATATGTTGGTGTTTGCCGCTACTTTACAGTGGACGACTTGTACATTCTGATGGGATCTGCAAACGTAAACCAGAGGTCCATGGACGGAGAGCGAGACACAGAGATTGCAATCGGATGCTACCAATCCGAAAACGATGGAGAAGAGATGCCCAATGGAAGAGACATTTCTACATTCCGATTATCGCTTTGGTATGAACACACGCGACGCTTCGAAGAGCTGTTCTTGGACCCCGAAAGCTTGCAATGCGTTCAAAGGGTTCGTTCAATAGCAGACGAAGCGTGGAAAACTTACAGCGGAGAAGAAGTGGCAGACATGGAAGGAGTCCACCTCGTAACGTACCCTGTGAAGGTAACTCAGGAGGGCTCAGTGGAGGATTTGGAAGAAAATGGAGGCCATTTTCCTGATACCAAATGCCCCATTAAGGGAAGAAGATCAATGCTCTTGCCGCCCATCTTCACAACTTAG

mRNA sequence

ATGGAGGGAAAGCAGAAGTTCTTCCACGGAACGCTCGAAGTTACCATCTTCCATGCAACAGCATACACACCGCCGTCACCTCTCGATTGCATATTTGCAGGAGGAAAGCGTAGCTATGTGACCGTCAAGATAGACAACAAGAGGTTGCCCAGACGAGTCACGACCCAACAGATTTTGAACGAAGCAAGTTTCATCAATGGTTTCTTTCCGCTTCTTATGGAAAATGGGAAGCCTAGCCCGGAGCTCAGACTTCGATTCATGCTGTGGTTTAAACCGGCAGTATATGAATTAAGCTGGAAAAAGATGCTGGGAAATGGAGAATACAAGGGGCTGAGGAATGCAACATTTCATCTAAGGTCCAATTGTCATGTAACTCTATATCAAGATGCTCACCACCTCCCTACTTTTCGACCTCCGTTTCACGGTTCGAGTGCACCAAGAAGGCTATGGGAAGATATATACAAGGCCATAGATAATGCAAAGCATTTGATTTATATTGCAGGCTGGTCCTTCAATCCAAAGATGGTGCTGGTAAGAGACTCTCAGACTGATATACCCTATGCTTTAGGAGTAAAGCTTGGTGAGCTGTTGAAGCAGAAGGCAGAGGAAGGTGTAGCTGTGAGAATATTGATTTGGGACGATGAAACATCCCTTCCCATCATCAAGAATGCCGGGATAATGAAAACACGCGATGAAGATGCTCGGGCATATTTCTCGCACTCAAAAGTTATATGCAAGTTGTGTCCCAAATTACACCCTATGTCCCCACCAATTTTCTCTCACCATCAGAAAACCATAGTTGTAGACGCTCAAACACACATCAATGCACAAAACAGGGAGATTATGAGTTTCATTGGGGGTTTAGATCTTTGTGATGGTCGCTATGACACTGATCAACATTCATTGTTCCATACTTTGAATATGGAATCCCACTGCTGTGATTTCTATCAGACAAGTATATCAGGTGCCAAGCTTCAAAAAGGAGGGCCAAGAGAGCCATGGCATGATGTTCATGCTTGTGTAACAGGTGAAGCTGCTTGGGATATATTAACAAACTTTGAGCAACGATGGACTAAGCAATCAGATGCTTCATTGTTAGTTCCCACAAACATCTTATTAAATCTGATGCCCCAGCTCGAAACAAACACTAACCCACAAAAGGATTGGAACGTGCAAGTTTTTCGATCTATTGACCACTTGTCTGCCAGCCAAGTATTCAGAAACTTGACAGTTGAACGAACCATCCATGAAGCTTATGTTGAAGCCATCAGGCGAGCGGATAGATTTATTTACATCGAAAACCAATATTTCATAGGAGGGTGCCACTTGTGGGACAAAGATCAACACTGCGGATGCACGAATTTGATACCGATTGAGATTGCACTCAAGGTGGCTAATAAGATAAAGGCAAGGGAGAGGTTTGCAGTTTATATAGTGATACCAATGTGGCCAGAAGGACCACCAGAGAGTGAATCAGTTGAGGATATGCTACATTGGACTAGGCAGACAATAACAATGATGTATAGACTGATTGGAGAAGCCATCCAAGAAACTGGGGAGATGGCCCACCCAAGAGACTACTTGAATTTCTTTTGCCTTGCAAACAGAGAAGAAGAGAGAAAATGGGACTTTGTTCCCCCACACAGTCCCCAACAGGCAACACAGTACTGGAATGCCCAACAACATAGGAGATTCATGGTCTATGTCCATTCCAAGGTCATGATAGTGGACGACTTGTACATTCTGATGGGATCTGCAAACGTAAACCAGAGGTCCATGGACGGAGAGCGAGACACAGAGATTGCAATCGGATGCTACCAATCCGAAAACGATGGAGAAGAGATGCCCAATGGAAGAGACATTTCTACATTCCGATTATCGCTTTGGTATGAACACACGCGACGCTTCGAAGAGCTGTTCTTGGACCCCGAAAGCTTGCAATGCGTTCAAAGGGTTCGTTCAATAGCAGACGAAGCGTGGAAAACTTACAGCGGAGAAGAAGTGGCAGACATGGAAGGAGTCCACCTCGTAACGTACCCTGTGAAGGTAACTCAGGAGGGCTCAGTGGAGGATTTGGAAGAAAATGGAGGCCATTTTCCTGATACCAAATGCCCCATTAAGGGAAGAAGATCAATGCTCTTGCCGCCCATCTTCACAACTTAG

Coding sequence (CDS)

ATGGAGGGAAAGCAGAAGTTCTTCCACGGAACGCTCGAAGTTACCATCTTCCATGCAACAGCATACACACCGCCGTCACCTCTCGATTGCATATTTGCAGGAGGAAAGCGTAGCTATGTGACCGTCAAGATAGACAACAAGAGGTTGCCCAGACGAGTCACGACCCAACAGATTTTGAACGAAGCAAGTTTCATCAATGGTTTCTTTCCGCTTCTTATGGAAAATGGGAAGCCTAGCCCGGAGCTCAGACTTCGATTCATGCTGTGGTTTAAACCGGCAGTATATGAATTAAGCTGGAAAAAGATGCTGGGAAATGGAGAATACAAGGGGCTGAGGAATGCAACATTTCATCTAAGGTCCAATTGTCATGTAACTCTATATCAAGATGCTCACCACCTCCCTACTTTTCGACCTCCGTTTCACGGTTCGAGTGCACCAAGAAGGCTATGGGAAGATATATACAAGGCCATAGATAATGCAAAGCATTTGATTTATATTGCAGGCTGGTCCTTCAATCCAAAGATGGTGCTGGTAAGAGACTCTCAGACTGATATACCCTATGCTTTAGGAGTAAAGCTTGGTGAGCTGTTGAAGCAGAAGGCAGAGGAAGGTGTAGCTGTGAGAATATTGATTTGGGACGATGAAACATCCCTTCCCATCATCAAGAATGCCGGGATAATGAAAACACGCGATGAAGATGCTCGGGCATATTTCTCGCACTCAAAAGTTATATGCAAGTTGTGTCCCAAATTACACCCTATGTCCCCACCAATTTTCTCTCACCATCAGAAAACCATAGTTGTAGACGCTCAAACACACATCAATGCACAAAACAGGGAGATTATGAGTTTCATTGGGGGTTTAGATCTTTGTGATGGTCGCTATGACACTGATCAACATTCATTGTTCCATACTTTGAATATGGAATCCCACTGCTGTGATTTCTATCAGACAAGTATATCAGGTGCCAAGCTTCAAAAAGGAGGGCCAAGAGAGCCATGGCATGATGTTCATGCTTGTGTAACAGGTGAAGCTGCTTGGGATATATTAACAAACTTTGAGCAACGATGGACTAAGCAATCAGATGCTTCATTGTTAGTTCCCACAAACATCTTATTAAATCTGATGCCCCAGCTCGAAACAAACACTAACCCACAAAAGGATTGGAACGTGCAAGTTTTTCGATCTATTGACCACTTGTCTGCCAGCCAAGTATTCAGAAACTTGACAGTTGAACGAACCATCCATGAAGCTTATGTTGAAGCCATCAGGCGAGCGGATAGATTTATTTACATCGAAAACCAATATTTCATAGGAGGGTGCCACTTGTGGGACAAAGATCAACACTGCGGATGCACGAATTTGATACCGATTGAGATTGCACTCAAGGTGGCTAATAAGATAAAGGCAAGGGAGAGGTTTGCAGTTTATATAGTGATACCAATGTGGCCAGAAGGACCACCAGAGAGTGAATCAGTTGAGGATATGCTACATTGGACTAGGCAGACAATAACAATGATGTATAGACTGATTGGAGAAGCCATCCAAGAAACTGGGGAGATGGCCCACCCAAGAGACTACTTGAATTTCTTTTGCCTTGCAAACAGAGAAGAAGAGAGAAAATGGGACTTTGTTCCCCCACACAGTCCCCAACAGGCAACACAGTACTGGAATGCCCAACAACATAGGAGATTCATGGTCTATGTCCATTCCAAGGTCATGATAGTGGACGACTTGTACATTCTGATGGGATCTGCAAACGTAAACCAGAGGTCCATGGACGGAGAGCGAGACACAGAGATTGCAATCGGATGCTACCAATCCGAAAACGATGGAGAAGAGATGCCCAATGGAAGAGACATTTCTACATTCCGATTATCGCTTTGGTATGAACACACGCGACGCTTCGAAGAGCTGTTCTTGGACCCCGAAAGCTTGCAATGCGTTCAAAGGGTTCGTTCAATAGCAGACGAAGCGTGGAAAACTTACAGCGGAGAAGAAGTGGCAGACATGGAAGGAGTCCACCTCGTAACGTACCCTGTGAAGGTAACTCAGGAGGGCTCAGTGGAGGATTTGGAAGAAAATGGAGGCCATTTTCCTGATACCAAATGCCCCATTAAGGGAAGAAGATCAATGCTCTTGCCGCCCATCTTCACAACTTAG

Protein sequence

MEGKQKFFHGTLEVTIFHATAYTPPSPLDCIFAGGKRSYVTVKIDNKRLPRRVTTQQILNEASFINGFFPLLMENGKPSPELRLRFMLWFKPAVYELSWKKMLGNGEYKGLRNATFHLRSNCHVTLYQDAHHLPTFRPPFHGSSAPRRLWEDIYKAIDNAKHLIYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQKAEEGVAVRILIWDDETSLPIIKNAGIMKTRDEDARAYFSHSKVICKLCPKLHPMSPPIFSHHQKTIVVDAQTHINAQNREIMSFIGGLDLCDGRYDTDQHSLFHTLNMESHCCDFYQTSISGAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTNILLNLMPQLETNTNPQKDWNVQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRADRFIYIENQYFIGGCHLWDKDQHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGPPESESVEDMLHWTRQTITMMYRLIGEAIQETGEMAHPRDYLNFFCLANREEERKWDFVPPHSPQQATQYWNAQQHRRFMVYVHSKVMIVDDLYILMGSANVNQRSMDGERDTEIAIGCYQSENDGEEMPNGRDISTFRLSLWYEHTRRFEELFLDPESLQCVQRVRSIADEAWKTYSGEEVADMEGVHLVTYPVKVTQEGSVEDLEENGGHFPDTKCPIKGRRSMLLPPIFTT
Homology
BLAST of Sgr016275 vs. NCBI nr
Match: XP_022148254.1 (phospholipase D alpha 4 [Momordica charantia])

HSP 1 Score: 1397.9 bits (3617), Expect = 0.0e+00
Identity = 659/759 (86.82%), Postives = 691/759 (91.04%), Query Frame = 0

Query: 1   MEGKQKFFHGTLEVTIFHATAYTPPSPLDCIFAGGKRSYVTVKIDNKRLPR--------- 60
           MEGKQ FFHGTLEVT+FHATAYTPPSPLDC+F+GG+R+YVTVKIDNK + +         
Sbjct: 3   MEGKQMFFHGTLEVTVFHATAYTPPSPLDCLFSGGRRTYVTVKIDNKEVAQTSHEYDRVW 62

Query: 61  ------------------------------RVTTQQILNEASFINGFFPLLMENGKPSPE 120
                                          +  QQIL EASF+NGFFPL MENGKPSPE
Sbjct: 63  NQTFQILCAYPSTSTVTITMKTSRSVLGKFHIQAQQILKEASFMNGFFPLTMENGKPSPE 122

Query: 121 LRLRFMLWFKPAVYELSWKKMLGNGEYKGLRNATFHLRSNCHVTLYQDAHHLPTFRPPFH 180
           LRLRFMLWFKPAVYELSWKKMLGNGEYKGLRNATF LRSNCHVTLYQDAHHLPTF+PPFH
Sbjct: 123 LRLRFMLWFKPAVYELSWKKMLGNGEYKGLRNATFPLRSNCHVTLYQDAHHLPTFQPPFH 182

Query: 181 GSSAPRRLWEDIYKAIDNAKHLIYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQKA 240
            SS+PRRLWED+YKAIDNAKHLIYIAGWSFNPKM+LVRDSQTDIPYALGVKLGELLKQKA
Sbjct: 183 SSSSPRRLWEDVYKAIDNAKHLIYIAGWSFNPKMLLVRDSQTDIPYALGVKLGELLKQKA 242

Query: 241 EEGVAVRILIWDDETSLPIIKNAGIMKTRDEDARAYFSHSKVICKLCPKLHPMSPPIFSH 300
           EEGVAVRI+IWDD TSLPIIKNAGIMKTRDEDA AYFSHSKVIC+LCPKLHPM+PPIFSH
Sbjct: 243 EEGVAVRIMIWDDATSLPIIKNAGIMKTRDEDAWAYFSHSKVICRLCPKLHPMAPPIFSH 302

Query: 301 HQKTIVVDAQTHINAQNREIMSFIGGLDLCDGRYDTDQHSLFHTLNMESHCCDFYQTSIS 360
           HQKTI VDAQ HINA+NREIMSFIGGLDLCDGRYDT+QHSLFHTLNMESHC DFYQTSIS
Sbjct: 303 HQKTITVDAQAHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNMESHCGDFYQTSIS 362

Query: 361 GAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTNILLNLMPQLET 420
           GAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPT+ILLNLMPQLET
Sbjct: 363 GAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLNLMPQLET 422

Query: 421 NTNPQKDWNVQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRADRFIYIENQYFIGGC 480
           NTNPQ DWNVQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRA+RFIYIENQYFIGGC
Sbjct: 423 NTNPQNDWNVQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIGGC 482

Query: 481 HLWDKDQHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGPPESESVEDMLHWTR 540
           HLWDKDQHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGPPESESV +MLHWTR
Sbjct: 483 HLWDKDQHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGPPESESVAEMLHWTR 542

Query: 541 QTITMMYRLIGEAIQETGEMAHPRDYLNFFCLANREEERKWDFVPPHSPQQATQYWNAQQ 600
           QT+ MMY+LIGEAIQETGE AHPRDYLNFFCLANREEE +WDFVPPHSP++ATQYWNAQQ
Sbjct: 543 QTMKMMYKLIGEAIQETGEKAHPRDYLNFFCLANREEEGEWDFVPPHSPERATQYWNAQQ 602

Query: 601 HRRFMVYVHSKVMIVDDLYILMGSANVNQRSMDGERDTEIAIGCYQSENDGEEMPNGRDI 660
           HRRFMVYVHSKVMIVDDLYIL+GSANVNQRSMDG RDTEIAIGC+Q E D EEMPNGRDI
Sbjct: 603 HRRFMVYVHSKVMIVDDLYILIGSANVNQRSMDGGRDTEIAIGCFQQEKDAEEMPNGRDI 662

Query: 661 STFRLSLWYEHTRRFEELFLDPESLQCVQRVRSIADEAWKTYSGEEVADMEGVHLVTYPV 720
           STFRLSLWYEHTRRFEELFL+PE+L CVQRVRSIADE+W+ YSGEE ADMEGVHLV YPV
Sbjct: 663 STFRLSLWYEHTRRFEELFLNPETLDCVQRVRSIADESWRIYSGEEAADMEGVHLVAYPV 722

BLAST of Sgr016275 vs. NCBI nr
Match: XP_038879261.1 (phospholipase D alpha 4 [Benincasa hispida])

HSP 1 Score: 1396.7 bits (3614), Expect = 0.0e+00
Identity = 653/759 (86.03%), Postives = 691/759 (91.04%), Query Frame = 0

Query: 1   MEGKQKFFHGTLEVTIFHATAYTPPSPLDCIFAGGKRSYVTVKIDNKRLPRR-------- 60
           MEGKQKFFHGTLEVT+FHATAYTPPSPLDCIF+GGKRSYVT+KIDNK + +         
Sbjct: 3   MEGKQKFFHGTLEVTVFHATAYTPPSPLDCIFSGGKRSYVTIKIDNKEVAQTSHEQDRVW 62

Query: 61  -------------------------------VTTQQILNEASFINGFFPLLMENGKPSPE 120
                                          +  QQIL EASFINGFFPLLMENGKPSPE
Sbjct: 63  NQTFRVLCAHPLTSTVTITMKTSRSVLGKFYIQAQQILKEASFINGFFPLLMENGKPSPE 122

Query: 121 LRLRFMLWFKPAVYELSWKKMLGNGEYKGLRNATFHLRSNCHVTLYQDAHHLPTFRPPFH 180
           L+LRFMLWFKPA+YELSWKKMLGNGEYKGLRNATF LRSNCHVTLYQDAHHLPTF+PPFH
Sbjct: 123 LKLRFMLWFKPAIYELSWKKMLGNGEYKGLRNATFPLRSNCHVTLYQDAHHLPTFQPPFH 182

Query: 181 GSSAPRRLWEDIYKAIDNAKHLIYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQKA 240
           GSS PRRLWED+YKAIDNAKHL+YIAGWSFNPKMVLVRDS TDIPYALGVKLGELLKQKA
Sbjct: 183 GSSTPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDSHTDIPYALGVKLGELLKQKA 242

Query: 241 EEGVAVRILIWDDETSLPIIKNAGIMKTRDEDARAYFSHSKVICKLCPKLHPMSPPIFSH 300
           EEGVAVRILIWDDETSLPIIKNAGIM T DEDARAYF HSKVIC+LCPKLHPMSPPIFSH
Sbjct: 243 EEGVAVRILIWDDETSLPIIKNAGIMNTHDEDARAYFLHSKVICRLCPKLHPMSPPIFSH 302

Query: 301 HQKTIVVDAQTHINAQNREIMSFIGGLDLCDGRYDTDQHSLFHTLNMESHCCDFYQTSIS 360
           HQKTI VDAQTHINA+NREIMSFIGGLDLCDGRYDT+QHSLFHTLN ESHC DFYQTSIS
Sbjct: 303 HQKTITVDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNRESHCSDFYQTSIS 362

Query: 361 GAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTNILLNLMPQLET 420
           GAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPT+ILL LMPQLE+
Sbjct: 363 GAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPQLES 422

Query: 421 NTNPQKDWNVQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRADRFIYIENQYFIGGC 480
           +TNPQKDWNVQVFRSIDHLSASQVFRN+T+ERTIHEAYVEAIRRA+RFIYIENQYFIGGC
Sbjct: 423 STNPQKDWNVQVFRSIDHLSASQVFRNMTIERTIHEAYVEAIRRAERFIYIENQYFIGGC 482

Query: 481 HLWDKDQHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGPPESESVEDMLHWTR 540
           HLWDKDQHCGCTNLIPIEIALKV NKIKARERF VYIVIPMWPEGPPESESVEDMLHWTR
Sbjct: 483 HLWDKDQHCGCTNLIPIEIALKVVNKIKARERFTVYIVIPMWPEGPPESESVEDMLHWTR 542

Query: 541 QTITMMYRLIGEAIQETGEMAHPRDYLNFFCLANREEERKWDFVPPHSPQQATQYWNAQQ 600
           QT+ MMYRLIGEAIQETGE AHPRDYLNFFCLANREEE KWDF+PP SPQ ATQYWN+QQ
Sbjct: 543 QTMAMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEEGKWDFIPPQSPQHATQYWNSQQ 602

Query: 601 HRRFMVYVHSKVMIVDDLYILMGSANVNQRSMDGERDTEIAIGCYQSENDGEEMPNGRDI 660
           HRRFM+YVHSKVMIVDDLYIL+GSANVNQRSMDG+RDTEIAIGCYQ ENDGEE PNGRDI
Sbjct: 603 HRRFMIYVHSKVMIVDDLYILIGSANVNQRSMDGDRDTEIAIGCYQLENDGEEFPNGRDI 662

Query: 661 STFRLSLWYEHTRRFEELFLDPESLQCVQRVRSIADEAWKTYSGEEVADMEGVHLVTYPV 720
           STFRLSLWYEHT+RFEE+FL+PESL+CVQRVRSI D++W+ YSGEEV DM+GVHLVTYPV
Sbjct: 663 STFRLSLWYEHTQRFEEVFLNPESLKCVQRVRSIGDKSWEIYSGEEVEDMKGVHLVTYPV 722

BLAST of Sgr016275 vs. NCBI nr
Match: XP_008447602.1 (PREDICTED: phospholipase D alpha 4 [Cucumis melo] >KAA0051141.1 phospholipase D alpha 4 [Cucumis melo var. makuwa] >TYK03757.1 phospholipase D alpha 4 [Cucumis melo var. makuwa])

HSP 1 Score: 1392.1 bits (3602), Expect = 0.0e+00
Identity = 653/759 (86.03%), Postives = 690/759 (90.91%), Query Frame = 0

Query: 1   MEGKQKFFHGTLEVTIFHATAYTPPSPLDCIFAGGKRSYVTVKIDNKRLPRR-------- 60
           ME KQKFFHG LEVT+FHATAYTPPSPLDC+FAGGKRSYVT+KIDNK + +         
Sbjct: 3   MEVKQKFFHGMLEVTVFHATAYTPPSPLDCLFAGGKRSYVTIKIDNKEVAQTSHEHDRVW 62

Query: 61  -------------------------------VTTQQILNEASFINGFFPLLMENGKPSPE 120
                                          +  QQIL EASFINGFFPLLMENGKPSPE
Sbjct: 63  NQTFRVLCAYPLTSTITITLKTARSVLGKFYIQAQQILKEASFINGFFPLLMENGKPSPE 122

Query: 121 LRLRFMLWFKPAVYELSWKKMLGNGEYKGLRNATFHLRSNCHVTLYQDAHHLPTFRPPFH 180
           L+LRFMLWFKPAVYELSWKKML NGEYKGLRNATF LRSNCHVTLYQDAHHLPTF+PPFH
Sbjct: 123 LKLRFMLWFKPAVYELSWKKMLANGEYKGLRNATFPLRSNCHVTLYQDAHHLPTFQPPFH 182

Query: 181 GSSAPRRLWEDIYKAIDNAKHLIYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQKA 240
           GSS PRRLWED+YKAIDNAKHL+YIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQKA
Sbjct: 183 GSSTPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQKA 242

Query: 241 EEGVAVRILIWDDETSLPIIKNAGIMKTRDEDARAYFSHSKVICKLCPKLHPMSPPIFSH 300
           EEGVAVRILIWDDETSLPIIKNAGIM T DEDARAYF HSKVIC+LCPKLHPMSPPIFSH
Sbjct: 243 EEGVAVRILIWDDETSLPIIKNAGIMNTHDEDARAYFLHSKVICRLCPKLHPMSPPIFSH 302

Query: 301 HQKTIVVDAQTHINAQNREIMSFIGGLDLCDGRYDTDQHSLFHTLNMESHCCDFYQTSIS 360
           HQK I+VDAQTHINAQNREIMSFIGGLDLCDGRYDT+QHSLFHTLN ESHC DFYQTSIS
Sbjct: 303 HQKIIIVDAQTHINAQNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCRDFYQTSIS 362

Query: 361 GAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTNILLNLMPQLET 420
           GAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPT+ILL LMPQLE+
Sbjct: 363 GAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPQLES 422

Query: 421 NTNPQKDWNVQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRADRFIYIENQYFIGGC 480
           NTNPQKDWNVQVFRSIDHLSASQ+FRN+T+ERTIHEAYVEAIRRA+RFIYIENQYFIGGC
Sbjct: 423 NTNPQKDWNVQVFRSIDHLSASQMFRNMTIERTIHEAYVEAIRRAERFIYIENQYFIGGC 482

Query: 481 HLWDKDQHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGPPESESVEDMLHWTR 540
           H WD+D+HCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEG PESESVEDMLHWTR
Sbjct: 483 HFWDRDRHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGSPESESVEDMLHWTR 542

Query: 541 QTITMMYRLIGEAIQETGEMAHPRDYLNFFCLANREEERKWDFVPPHSPQQATQYWNAQQ 600
           QT+TMMYRLIGEAIQETGE AHPRDYLNFFCLANREEERKWDF+PPHSPQ ATQYWNAQQ
Sbjct: 543 QTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFIPPHSPQHATQYWNAQQ 602

Query: 601 HRRFMVYVHSKVMIVDDLYILMGSANVNQRSMDGERDTEIAIGCYQSENDGEEMPNGRDI 660
           HRRFM+YVHSKVMIVDDLYIL+GSANVNQRSMDGERDTEIAIGCYQ ENDGEE+PNGRDI
Sbjct: 603 HRRFMIYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAIGCYQIENDGEELPNGRDI 662

Query: 661 STFRLSLWYEHTRRFEELFLDPESLQCVQRVRSIADEAWKTYSGEEVADMEGVHLVTYPV 720
           S FRLSLWYEHTR FEE+FL+PESL+CVQRVRSI D++WK YSGEEV DM+GVHLVTYPV
Sbjct: 663 SRFRLSLWYEHTRGFEEVFLNPESLKCVQRVRSIGDKSWKIYSGEEVEDMKGVHLVTYPV 722

BLAST of Sgr016275 vs. NCBI nr
Match: XP_004146826.1 (phospholipase D alpha 4 [Cucumis sativus] >KAE8651293.1 hypothetical protein Csa_001191 [Cucumis sativus])

HSP 1 Score: 1386.3 bits (3587), Expect = 0.0e+00
Identity = 649/759 (85.51%), Postives = 689/759 (90.78%), Query Frame = 0

Query: 1   MEGKQKFFHGTLEVTIFHATAYTPPSPLDCIFAGGKRSYVTVKIDNKRLPRR-------- 60
           ME KQKFFHGTLEVT+FHATAY P SPLDC+FAGGK SYVT+KIDNK + +         
Sbjct: 3   MEVKQKFFHGTLEVTVFHATAYAPSSPLDCLFAGGKCSYVTIKIDNKEVAQTSHEQDRVW 62

Query: 61  -------------------------------VTTQQILNEASFINGFFPLLMENGKPSPE 120
                                          +  QQIL EASFINGFFPLLMENGKPSPE
Sbjct: 63  NQTFRVLCAHPLTSTVTITLKTSRSVLGKFYIQAQQILKEASFINGFFPLLMENGKPSPE 122

Query: 121 LRLRFMLWFKPAVYELSWKKMLGNGEYKGLRNATFHLRSNCHVTLYQDAHHLPTFRPPFH 180
           L+LRFMLWFKPAVYELSWKKMLGNGEYKGLRNATF LRSNCHVTLYQDAHHLPTF+PPFH
Sbjct: 123 LKLRFMLWFKPAVYELSWKKMLGNGEYKGLRNATFPLRSNCHVTLYQDAHHLPTFQPPFH 182

Query: 181 GSSAPRRLWEDIYKAIDNAKHLIYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQKA 240
           GSS PRRLWED+YKAIDNAKHL+YIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQKA
Sbjct: 183 GSSTPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQKA 242

Query: 241 EEGVAVRILIWDDETSLPIIKNAGIMKTRDEDARAYFSHSKVICKLCPKLHPMSPPIFSH 300
           +EGVAVRILIWDDETSLPIIKNAGIM T DEDARAYF HSKVIC+LCPKLHPMSPPIFSH
Sbjct: 243 DEGVAVRILIWDDETSLPIIKNAGIMNTHDEDARAYFLHSKVICRLCPKLHPMSPPIFSH 302

Query: 301 HQKTIVVDAQTHINAQNREIMSFIGGLDLCDGRYDTDQHSLFHTLNMESHCCDFYQTSIS 360
           HQKTI+VDAQTHINAQNREIMSFIGGLDLCDGRYDT+QHSLFHTLN ESHC DFYQTSIS
Sbjct: 303 HQKTIIVDAQTHINAQNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCRDFYQTSIS 362

Query: 361 GAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTNILLNLMPQLET 420
           GAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPT+ILL LMPQLE+
Sbjct: 363 GAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPQLES 422

Query: 421 NTNPQKDWNVQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRADRFIYIENQYFIGGC 480
           NTNPQKDWNVQVFRSIDHLSASQ+FRN+T+ERTIHEAYVEAIRRA+RFIYIENQYFIGGC
Sbjct: 423 NTNPQKDWNVQVFRSIDHLSASQMFRNMTIERTIHEAYVEAIRRAERFIYIENQYFIGGC 482

Query: 481 HLWDKDQHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGPPESESVEDMLHWTR 540
           H WD+DQHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEG PESESVEDMLHWTR
Sbjct: 483 HFWDRDQHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGSPESESVEDMLHWTR 542

Query: 541 QTITMMYRLIGEAIQETGEMAHPRDYLNFFCLANREEERKWDFVPPHSPQQATQYWNAQQ 600
           QT+TMMYRLIGEAIQETGE AHPRDYLNFFCLANREEERKWDF+PPHSPQ ATQYWNAQQ
Sbjct: 543 QTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFIPPHSPQHATQYWNAQQ 602

Query: 601 HRRFMVYVHSKVMIVDDLYILMGSANVNQRSMDGERDTEIAIGCYQSENDGEEMPNGRDI 660
           HRRFM+YVHSKVMIVDDLYIL+GSANVNQRSMDGERDTEIA+GCYQ EN+GEE+PNGRDI
Sbjct: 603 HRRFMIYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAMGCYQIENEGEELPNGRDI 662

Query: 661 STFRLSLWYEHTRRFEELFLDPESLQCVQRVRSIADEAWKTYSGEEVADMEGVHLVTYPV 720
           S FRLSLWYEHT  FEE+FL+PESL+CV+RVRSI D++WK YSGEEV DM+GVH+VTYPV
Sbjct: 663 SKFRLSLWYEHTGGFEEVFLNPESLKCVERVRSIGDKSWKIYSGEEVEDMKGVHMVTYPV 722

BLAST of Sgr016275 vs. NCBI nr
Match: XP_023525777.1 (phospholipase D alpha 4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1385.2 bits (3584), Expect = 0.0e+00
Identity = 648/759 (85.38%), Postives = 693/759 (91.30%), Query Frame = 0

Query: 1   MEGKQKFFHGTLEVTIFHATAYTPPSPLDCIFAGGKRSYVTVKIDNKRLPR--------- 60
           + GK KFFHGTLEVT+FHAT YTP SPLDCIF+GGK+SYVT+KI+N  + R         
Sbjct: 63  VHGKHKFFHGTLEVTVFHATTYTPSSPLDCIFSGGKQSYVTIKINNIEVARTNHEYDRVW 122

Query: 61  ------------------------------RVTTQQILNEASFINGFFPLLMENGKPSPE 120
                                         ++  QQIL E+SFINGFFPLLMENGKPSPE
Sbjct: 123 NQTFRVLCAHPLTSTITITMRTSRSVLGRFQIQAQQILKESSFINGFFPLLMENGKPSPE 182

Query: 121 LRLRFMLWFKPAVYELSWKKMLGNGEYKGLRNATFHLRSNCHVTLYQDAHHLPTFRPPFH 180
           LRLRFMLWF+PAVYELSWKK+L NGEYKGLRNATF LRSNCHVTLYQDAHH+ TF+PPFH
Sbjct: 183 LRLRFMLWFQPAVYELSWKKVLENGEYKGLRNATFPLRSNCHVTLYQDAHHVSTFQPPFH 242

Query: 181 GSSAPRRLWEDIYKAIDNAKHLIYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQKA 240
           GSSAPRRLWED+YKAIDNAKHL+YIAGWSFNPKMVLVRD QTDIPYALGVKLGELLKQKA
Sbjct: 243 GSSAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDPQTDIPYALGVKLGELLKQKA 302

Query: 241 EEGVAVRILIWDDETSLPIIKNAGIMKTRDEDARAYFSHSKVICKLCPKLHPMSPPIFSH 300
           EEGVAVR++IWDDETSLP+IKNAGIMKT DEDARAYFS+SKV+C+LCPKLHPMSPPIFSH
Sbjct: 303 EEGVAVRLMIWDDETSLPVIKNAGIMKTHDEDARAYFSNSKVVCRLCPKLHPMSPPIFSH 362

Query: 301 HQKTIVVDAQTHINAQNREIMSFIGGLDLCDGRYDTDQHSLFHTLNMESHCCDFYQTSIS 360
           HQKTI VDAQTHINA+NREIMSFIGGLDLCDGRYDT+QHSLFHTLN ESHCCDFYQTSIS
Sbjct: 363 HQKTITVDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCCDFYQTSIS 422

Query: 361 GAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTNILLNLMPQLET 420
           GAKLQKGGPREPWHDVHA VTGEAAWDILTNFEQRWTKQSDASLLVPT+ILL LMP LE+
Sbjct: 423 GAKLQKGGPREPWHDVHARVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPPLES 482

Query: 421 NTNPQKDWNVQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRADRFIYIENQYFIGGC 480
           NTNPQKDWNVQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRA+RFIYIENQYFIGGC
Sbjct: 483 NTNPQKDWNVQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIGGC 542

Query: 481 HLWDKDQHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGPPESESVEDMLHWTR 540
           HLWDKDQHCGCTNLIPIEIALKVA KI+ARE+FAVY+VIPMWPEGPPESESVEDMLHWTR
Sbjct: 543 HLWDKDQHCGCTNLIPIEIALKVATKIRAREKFAVYVVIPMWPEGPPESESVEDMLHWTR 602

Query: 541 QTITMMYRLIGEAIQETGEMAHPRDYLNFFCLANREEERKWDFVPPHSPQQATQYWNAQQ 600
           QT+TMMYRLIGEAIQETGE AHPRDYLNFFCLANREEERKWDFVPPHSPQ AT+YW+AQQ
Sbjct: 603 QTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFVPPHSPQHATEYWSAQQ 662

Query: 601 HRRFMVYVHSKVMIVDDLYILMGSANVNQRSMDGERDTEIAIGCYQSENDGEEMPNGRDI 660
           HRRFMVYVHSKVMIVDDLYIL+GSANVNQRSMDGERDTEIAIGCYQ ENDG+E+PNGRDI
Sbjct: 663 HRRFMVYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAIGCYQLENDGKELPNGRDI 722

Query: 661 STFRLSLWYEHTRRFEELFLDPESLQCVQRVRSIADEAWKTYSGEEVADMEGVHLVTYPV 720
           STFRLSLWYEHT+RFEE+FL+PE+LQCV+RVRSIADE+WK YSGEEVADM+GVHLVTYPV
Sbjct: 723 STFRLSLWYEHTQRFEEVFLNPENLQCVERVRSIADESWKIYSGEEVADMKGVHLVTYPV 782

BLAST of Sgr016275 vs. ExPASy Swiss-Prot
Match: Q9C888 (Phospholipase D alpha 4 OS=Arabidopsis thaliana OX=3702 GN=PLDALPHA4 PE=2 SV=1)

HSP 1 Score: 891.3 bits (2302), Expect = 7.0e-258
Identity = 424/774 (54.78%), Postives = 563/774 (72.74%), Query Frame = 0

Query: 1   MEGKQKFFHGTLEVTIFHATAYTPPSPLDCIFAGGKRSYVTVKIDNKRLPR--------- 60
           +E ++K+FHGTLE+TIF AT ++PP P +CI    K +YVT+KI+ K++ +         
Sbjct: 3   LEEQKKYFHGTLEITIFDATPFSPPFPFNCICTKPKAAYVTIKINKKKVAKTSSEYDRIW 62

Query: 61  -------------------------------RVTTQQIL-NEASFINGFFPLLMENGKPS 120
                                          R++ +QIL + ++ INGFFPL+ +NG   
Sbjct: 63  NQTFQILCAHPVTDTTITITLKTRCSVLGRFRISAEQILTSNSAVINGFFPLIADNGSTK 122

Query: 121 PELRLRFMLWFKPAVYELSWKKMLGNGEYKGLRNATFHLRSNCHVTLYQDAHHLPTFRP- 180
             L+L+ ++WF+PA  E  W + L    ++G+RNA+F  RSNC V LYQDAHH  TF P 
Sbjct: 123 RNLKLKCLMWFRPAYLEPGWCRALEEASFQGIRNASFPQRSNCRVVLYQDAHHKATFDPR 182

Query: 181 ----PFHGSSAPRRLWEDIYKAIDNAKHLIYIAGWSFNPKMVLVRDSQTDIPYALGVKLG 240
               PF+     R LWED+YKAI++A+HL+YIAGW+ NP +VLVRD++T+IP+A+GV +G
Sbjct: 183 VDDVPFNA----RNLWEDVYKAIESARHLVYIAGWALNPNLVLVRDNETEIPHAVGVTVG 242

Query: 241 ELLKQKAEEGVAVRILIWDDETSLPIIKNAGIMKTRDEDARAYFSHSKVICKLCPKLHPM 300
           ELLK+K+EEGVAVR+++W+DETSLP+IKN G+M+T  E A AYF ++ V+C+LCP+LH  
Sbjct: 243 ELLKRKSEEGVAVRVMLWNDETSLPMIKNKGVMRTNVERALAYFRNTNVVCRLCPRLHKK 302

Query: 301 SPPIFSHHQKTIVVDAQ-THINAQNREIMSFIGGLDLCDGRYDTDQHSLFHTLNMESHCC 360
            P  F+HHQKTI +D + T+ + + REIMSF+GG DLCDGRYDT++HSLF TL  E+   
Sbjct: 303 LPTAFAHHQKTITLDTRVTNSSTKEREIMSFLGGFDLCDGRYDTEEHSLFRTLGTEA--- 362

Query: 361 DFYQTSISGAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTNILL 420
           DFYQTS++GAKL +GGPREPWHD H  V G AAWD+L NFEQRWTKQ + S+LV T+ + 
Sbjct: 363 DFYQTSVAGAKLSRGGPREPWHDCHVSVVGGAAWDVLKNFEQRWTKQCNPSVLVNTSGIR 422

Query: 421 NLMPQLETNTNPQKDWNVQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRADRFIYIE 480
           NL+          + WNVQV RSIDH+SA+++ R L VE+++H+ YV AIR+A+RFIYIE
Sbjct: 423 NLVNLTGPTEENNRKWNVQVLRSIDHISATEMPRGLPVEKSVHDGYVAAIRKAERFIYIE 482

Query: 481 NQYFIGGCHLWD--KDQHC-GCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGPPES 540
           NQYF+G C  W+   D+ C GCTNLIP+EIALK+A KI+ARERFAVYIVIPMWPEGPPES
Sbjct: 483 NQYFMGSCDHWESKNDKICSGCTNLIPVEIALKIAAKIRARERFAVYIVIPMWPEGPPES 542

Query: 541 ESVEDMLHWTRQTITMMYRLIGEAIQETGEMAHPRDYLNFFCLANREEERKWDFVPPHSP 600
           E+VE++LHWTR+T++MMY++IGEAI E G+ +HPRDYLNFFCLANREE+R  +F    SP
Sbjct: 543 ETVEEILHWTRETMSMMYQIIGEAIWEVGDKSHPRDYLNFFCLANREEKRDGEFEAVSSP 602

Query: 601 QQATQYWNAQQHRRFMVYVHSKVMIVDDLYILMGSANVNQRSMDGERDTEIAIGCYQSEN 660
            Q T YWNAQ++RRFMVYVHSK+MIVDD YIL+GSAN+NQRSMDG RDTEIAIGCYQ+  
Sbjct: 603 HQKTHYWNAQRNRRFMVYVHSKLMIVDDTYILIGSANINQRSMDGCRDTEIAIGCYQTNT 662

Query: 661 DGEEMPNGRDISTFRLSLWYEHT----RRFEELFLDPESLQCVQRVRSIADEAWKTYSGE 720
           +     N  +I  +RLSLWYEHT       +    +PESL+CV+ +R+I ++ W+ YSG+
Sbjct: 663 N-----NTNEIQAYRLSLWYEHTGGKITADDLSSSEPESLECVRGLRTIGEQMWEIYSGD 722

BLAST of Sgr016275 vs. ExPASy Swiss-Prot
Match: Q43270 (Phospholipase D alpha 1 OS=Zea mays OX=4577 GN=PLD1 PE=2 SV=1)

HSP 1 Score: 598.6 bits (1542), Expect = 9.4e-170
Identity = 316/718 (44.01%), Postives = 435/718 (60.58%), Query Frame = 0

Query: 41  TVKIDN----KRLPRRVTTQQILNEASFINGFFPLLMENGKPSPELRLRFMLWFKPAVYE 100
           TVKIDN      + R     Q L     I+ +  +  EN +P  + ++   L +     +
Sbjct: 98  TVKIDNSIGASLIGRAYLAVQDLLGGEEIDKWLEISDENREPVGDSKIHVKLQYFDVGKD 157

Query: 101 LSWKKMLGNGEYKGLRNATFHLRSNCHVTLYQDAHHLPTFRPPFHGSSA----PRRLWED 160
            +W + + + +Y G+    F  R  C VTLYQDAH    F P    +      P R WED
Sbjct: 158 RNWARGVRSTKYPGVPYTFFSQRQGCKVTLYQDAHVPDNFVPRIQLADGKNYEPHRCWED 217

Query: 161 IYKAIDNAKHLIYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQKAEEGVAVRILIW 220
           I+ AI  A+HLIYI GWS   ++ LVRD+    P    V LGELLK+KA EGV V +L+W
Sbjct: 218 IFDAISKAQHLIYITGWSVYTEITLVRDTNRPKPGG-DVTLGELLKRKASEGVRVLMLVW 277

Query: 221 DDETSLPIIKNAGIMKTRDEDARAYFSHSKVICKLCPKLHPMS---------PPIFSHHQ 280
           DD TS+ ++K  G+M T DE+   YF  + V C LCP+    S           +F+HHQ
Sbjct: 278 DDRTSVGLLKKDGLMATHDEETANYFHGTDVNCVLCPRNPDDSGSFVQDLQISTMFTHHQ 337

Query: 281 KTIVVDAQ-THINAQNREIMSFIGGLDLCDGRYDTDQHSLFHTLNMESHCCDFYQTSISG 340
           K +VVD +  +  +Q R I+SFIGG+DLCDGRYDT  HSLF TL+   H  DF+Q +  G
Sbjct: 338 KIVVVDHEMPNQGSQQRRIVSFIGGIDLCDGRYDTQYHSLFRTLDTVHH-DDFHQPNFEG 397

Query: 341 AKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTNILLNLM--PQLE 400
             ++KGGPREPWHD+H+ + G  AWD+L NFEQRW KQ    LLV    L +++  P   
Sbjct: 398 GSIKKGGPREPWHDIHSRLEGPIAWDVLYNFEQRWRKQGGKDLLVRLRDLPDIIIPPSPV 457

Query: 401 TNTNPQKDWNVQVFRSIDHLSASQV--------------FRNLTVERTIHEAYVEAIRRA 460
                ++ WNVQ+FRSID  +A                  ++  ++R+I +AYV AIRRA
Sbjct: 458 MFPEDRETWNVQLFRSIDGGAAFGFPETPEEAARAGLVSGKDQIIDRSIQDAYVNAIRRA 517

Query: 461 DRFIYIENQYFIGGCHLWD----KDQHCGCTNLIPIEIALKVANKIKARERFAVYIVIPM 520
             FIYIENQYF+G  + W     K +  G  +LIP E++LK+ +KI+A ERF VY+V+PM
Sbjct: 518 KNFIYIENQYFLGSSYGWKPEGIKPEEIGALHLIPKELSLKIVSKIEAGERFTVYVVVPM 577

Query: 521 WPEGPPESESVEDMLHWTRQTITMMYRLIGEAIQETGEMAHPRDYLNFFCLANREEERKW 580
           WPEG PES SV+ +L W R+T+ MMY  I +A++  G  A+P+DYL FFCL NRE +++ 
Sbjct: 578 WPEGVPESASVQAILDWQRRTMEMMYTDIAQALEANGIEANPKDYLTFFCLGNREVKQEG 637

Query: 581 DFVPPHSPQQATQYWNAQQHRRFMVYVHSKVMIVDDLYILMGSANVNQRSMDGERDTEIA 640
           ++ P   P+  T Y  AQ+ RRFM+YVH+K+MIVDD YI++GSAN+NQRSMDG RD+EIA
Sbjct: 638 EYEPEEHPEPDTDYIRAQEARRFMIYVHTKMMIVDDEYIIIGSANINQRSMDGARDSEIA 697

Query: 641 IGCYQSENDGEEMPNGRDISTFRLSLWYEHTRRFEELFLDPESLQCVQRVRSIADEAWKT 700
           +G YQ  +     P    I  FR+SLWYEH    E++F  PES++CVQ+V  +A++ W  
Sbjct: 698 MGAYQPYHLATRQPARGQIHGFRMSLWYEHLGMLEDVFQRPESVECVQKVNEVAEKYWDL 757

Query: 701 YSGEEVADMEGVHLVTYPVKVTQEGSVEDLEENGGHFPDTKCPIKGRRSMLLPPIFTT 721
           YS +++      HL++YP+ VT +GSV +L     +FPDT+  + G +S  LPPI TT
Sbjct: 758 YSSDDLEQDLPGHLLSYPIGVTADGSVTEL-PGMENFPDTRARVLGNKSDYLPPILTT 812

BLAST of Sgr016275 vs. ExPASy Swiss-Prot
Match: Q43007 (Phospholipase D alpha 1 OS=Oryza sativa subsp. japonica OX=39947 GN=PLD1 PE=1 SV=2)

HSP 1 Score: 597.0 bits (1538), Expect = 2.7e-169
Identity = 318/718 (44.29%), Postives = 433/718 (60.31%), Query Frame = 0

Query: 41  TVKIDN----KRLPRRVTTQQILNEASFINGFFPLLMENGKPSPELRLRFMLWFKPAVYE 100
           TVKIDN      + R     Q L     I+ +  +   N +P  E ++   L +     +
Sbjct: 98  TVKIDNPIGATNIGRAYLPVQELLNGEEIDRWLDICDNNREPVGESKIHVKLQYFDVSKD 157

Query: 101 LSWKKMLGNGEYKGLRNATFHLRSNCHVTLYQDAHHLPTFRPPFHGSSA----PRRLWED 160
            +W + + + +Y G+    F  R  C VTLYQDAH    F P    +      P R WED
Sbjct: 158 RNWARGVRSTKYPGVPYTFFSQRQGCKVTLYQDAHVPDNFIPKIPLADGKNYEPHRCWED 217

Query: 161 IYKAIDNAKHLIYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQKAEEGVAVRILIW 220
           I+ AI NA+HLIYI GWS   ++ LVRDS    P    V LGELLK+KA EGV V +L+W
Sbjct: 218 IFDAISNAQHLIYITGWSVYTEITLVRDSNRPKPGG-DVTLGELLKKKASEGVRVLMLVW 277

Query: 221 DDETSLPIIKNAGIMKTRDEDARAYFSHSKVICKLCPKLHPMSPPI---------FSHHQ 280
           DD TS+ ++K  G+M T DE+   YF  S V C LCP+    S  I         F+HHQ
Sbjct: 278 DDRTSVGLLKRDGLMATHDEETENYFHGSDVNCVLCPRNPDDSGSIVQDLSISTMFTHHQ 337

Query: 281 KTIVVDAQ-THINAQNREIMSFIGGLDLCDGRYDTDQHSLFHTLNMESHCCDFYQTSISG 340
           K +VVD +  +  +Q R I+SF+GGLDLCDGRYDT  HSLF TL+  +H  DF+Q + + 
Sbjct: 338 KIVVVDHELPNQGSQQRRIVSFVGGLDLCDGRYDTQYHSLFRTLD-STHHDDFHQPNFAT 397

Query: 341 AKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTNILLN--LMPQLE 400
           A ++KGGPREPWHD+H+ + G  AWD+L NFEQRW KQ    LL+    L +  + P   
Sbjct: 398 ASIKKGGPREPWHDIHSRLEGPIAWDVLYNFEQRWRKQGGKDLLLQLRDLSDTIIPPSPV 457

Query: 401 TNTNPQKDWNVQVFRSIDHLSASQV--------------FRNLTVERTIHEAYVEAIRRA 460
                ++ WNVQ+FRSID  +A                  ++  ++R+I +AY+ AIRRA
Sbjct: 458 MFPEDRETWNVQLFRSIDGGAAFGFPDTPEEAAKAGLVSGKDQIIDRSIQDAYIHAIRRA 517

Query: 461 DRFIYIENQYFIGGCHLWD----KDQHCGCTNLIPIEIALKVANKIKARERFAVYIVIPM 520
             FIYIENQYF+G  + W     K +  G  +LIP E+ALKV +KI+A ERF VY+V+PM
Sbjct: 518 KNFIYIENQYFLGSSYAWKPEGIKPEDIGALHLIPKELALKVVSKIEAGERFTVYVVVPM 577

Query: 521 WPEGPPESESVEDMLHWTRQTITMMYRLIGEAIQETGEMAHPRDYLNFFCLANREEERKW 580
           WPEG PES SV+ +L W R+T+ MMY  I EA+Q  G  A+P+DYL FFCL NRE ++  
Sbjct: 578 WPEGVPESGSVQAILDWQRRTMEMMYTDITEALQAKGIEANPKDYLTFFCLGNREVKQAG 637

Query: 581 DFVPPHSPQQATQYWNAQQHRRFMVYVHSKVMIVDDLYILMGSANVNQRSMDGERDTEIA 640
           ++ P   P+  T Y  AQ+ RRFM+YVH+K+MIVDD YI++GSAN+NQRSMDG RD+EIA
Sbjct: 638 EYQPEEQPEADTDYSRAQEARRFMIYVHTKMMIVDDEYIIIGSANINQRSMDGARDSEIA 697

Query: 641 IGCYQSENDGEEMPNGRDISTFRLSLWYEHTRRFEELFLDPESLQCVQRVRSIADEAWKT 700
           +G YQ  +     P    I  FR++LWYEH    +++F  PESL+CVQ+V  IA++ W  
Sbjct: 698 MGGYQPYHLATRQPARGQIHGFRMALWYEHLGMLDDVFQRPESLECVQKVNRIAEKYWDM 757

Query: 701 YSGEEVADMEGVHLVTYPVKVTQEGSVEDLEENGGHFPDTKCPIKGRRSMLLPPIFTT 721
           YS +++      HL++YP+ V  +G V +L     +FPDT+  + G +S  +PPI T+
Sbjct: 758 YSSDDLQQDLPGHLLSYPIGVASDGVVTEL-PGMEYFPDTRARVLGAKSDYMPPILTS 812

BLAST of Sgr016275 vs. ExPASy Swiss-Prot
Match: P93400 (Phospholipase D alpha 1 OS=Nicotiana tabacum OX=4097 GN=PLD1 PE=2 SV=2)

HSP 1 Score: 584.3 bits (1505), Expect = 1.8e-165
Identity = 314/720 (43.61%), Postives = 431/720 (59.86%), Query Frame = 0

Query: 41  TVKIDN----KRLPRRVTTQQILNEASFINGFFPLLMENGKPSPE-LRLRFMLWFKPAVY 100
           TVK DN      + R     + L E   I+ +  +L     P  E  ++   L F     
Sbjct: 93  TVKDDNPIGATLIGRAYVPVEELLEGEEIDKWVEILDREMNPIAEGSKIHVKLQFFDVSR 152

Query: 101 ELSWKKMLGNGEYKGLRNATFHLRSNCHVTLYQDAHHLPTFRP--PFHGSS--APRRLWE 160
           + +W++ + + +Y G+    F  R+ C V+LYQDAH    F P  P  G     P R WE
Sbjct: 153 DPNWERGIRSSKYPGVPYTFFAQRTGCRVSLYQDAHVPDNFIPKIPLSGGKYYEPHRCWE 212

Query: 161 DIYKAIDNAKHLIYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQKAEEGVAVRILI 220
           DI+ AI NAKHLIYI GWS   ++ LVRDS+   P    + LGELLK+KA EGV V +L+
Sbjct: 213 DIFDAIINAKHLIYITGWSVYTEITLVRDSRRQKPGG-DITLGELLKKKASEGVKVLMLV 272

Query: 221 WDDETSLPIIKNAGIMKTRDEDARAYFSHSKVICKLCPKLHPMSPPI---------FSHH 280
           WDD TS+ ++K  G+M T D++   +F  ++V C LCP+       I         F+HH
Sbjct: 273 WDDRTSVGLLKKDGLMATHDQETEQFFQGTEVNCVLCPRNPDDGGSIVQSLQIGTMFTHH 332

Query: 281 QKTIVVDAQTHI-NAQNREIMSFIGGLDLCDGRYDTDQHSLFHTLNMESHCCDFYQTSIS 340
           QK +VVD++     ++ R I+SF+GG+DLCDGRYDT  HSLF TL+  +H  DF+Q +  
Sbjct: 333 QKIVVVDSELPSGESEKRRILSFVGGIDLCDGRYDTPFHSLFRTLD-TAHHDDFHQPNFP 392

Query: 341 GAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTNILLNLM--PQL 400
              + KGGPREPWHD+H+ + G  AWD+L NFEQRW KQ    +LV    L +++  P  
Sbjct: 393 DGAITKGGPREPWHDIHSRLEGPIAWDVLFNFEQRWRKQGGKDVLVNFRELDDIIIPPSP 452

Query: 401 ETNTNPQKDWNVQVFRSIDHLSASQV--------------FRNLTVERTIHEAYVEAIRR 460
             + +  + WNVQ+FRSID  +A                   +  ++R+I +AY+ AIRR
Sbjct: 453 VMHLDDSETWNVQLFRSIDEGAAFGFPETPEDAAKAGLVSGXDNIIDRSIQDAYIHAIRR 512

Query: 461 ADRFIYIENQYFIGGCHLWDKD----QHCGCTNLIPIEIALKVANKIKARERFAVYIVIP 520
           A  FIYIENQYF+G  + W  D    +  G  ++IP E+ALK+ +KI+A ERF VY+V+P
Sbjct: 513 AKNFIYIENQYFLGSSYDWQSDDIKVEDIGALHVIPKELALKIVSKIEAGERFTVYVVVP 572

Query: 521 MWPEGPPESESVEDMLHWTRQTITMMYRLIGEAIQETGEMAHPRDYLNFFCLANREEERK 580
           MWPEG PES SV+ +L W R+T+ MMY+ I +A+   G    PR+YL FFC+ NRE ++ 
Sbjct: 573 MWPEGIPESASVQAILDWQRRTMEMMYKHIVQALNAKGIEEDPRNYLTFFCIGNREVKKS 632

Query: 581 WDFVPPHSPQQATQYWNAQQHRRFMVYVHSKVMIVDDLYILMGSANVNQRSMDGERDTEI 640
             + P  +P+  + Y  AQ+ RRFM+YVHSK+MIVDD YI++GSAN+NQRSMDG RD+EI
Sbjct: 633 GAYEPSETPEPDSDYIRAQEARRFMIYVHSKMMIVDDEYIIVGSANINQRSMDGARDSEI 692

Query: 641 AIGCYQSENDGEEMPNGRDISTFRLSLWYEHTRRFEELFLDPESLQCVQRVRSIADEAWK 700
           A+G YQ  +     P    I  FR++LWYEH    +E FL PES +CV +V  +AD+ W 
Sbjct: 693 AMGAYQPHHLATREPARGQIHGFRMALWYEHLGMLDETFLHPESEECVSKVNRMADKYWD 752

Query: 701 TYSGEEV-ADMEGVHLVTYPVKVTQEGSVEDLEENGGHFPDTKCPIKGRRSMLLPPIFTT 721
            YS E +  D+ G HL+ YP+ V  EG V +L     HFPDTK  + G +S  LPPI TT
Sbjct: 753 LYSSESLERDLPG-HLLRYPIGVASEGDVTEL-PGAEHFPDTKARVLGTKSDYLPPILTT 808

BLAST of Sgr016275 vs. ExPASy Swiss-Prot
Match: Q41142 (Phospholipase D alpha 1 OS=Ricinus communis OX=3988 GN=PLD1 PE=1 SV=1)

HSP 1 Score: 583.6 bits (1503), Expect = 3.1e-165
Identity = 314/721 (43.55%), Postives = 438/721 (60.75%), Query Frame = 0

Query: 41  TVKIDN----KRLPRRVTTQQILNEASFINGFFPLLMENGKP-SPELRLRFMLWFKPAVY 100
           TVK DN      + R     + L +   I+ +  +L E+  P     ++   L +     
Sbjct: 93  TVKDDNPIGATLIGRAYVPVEELLDGEEIDRWVEILDEDKNPVHSGSKIHVKLQYFEVTK 152

Query: 101 ELSWKKMLGNGEYKGLRNATFHLRSNCHVTLYQDAHHLPTFRP--PFHGSS--APRRLWE 160
           + +W + + + +Y G+    F  R  C V+LYQDAH    F P  P  G +   P R WE
Sbjct: 153 DRNWGQGIRSSKYPGVPYTYFSQRQGCKVSLYQDAHIPDKFVPQIPLAGGNYYEPHRCWE 212

Query: 161 DIYKAIDNAKHLIYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQKAEEGVAVRILI 220
           D++ AI NAKHLIYI GWS   ++ L+RDS+   P    + LGELLK+KA EGV V +L+
Sbjct: 213 DVFDAITNAKHLIYITGWSVYTEISLIRDSRRPKPGG-DITLGELLKKKASEGVRVLMLV 272

Query: 221 WDDETSLPIIKNAGIMKTRDEDARAYFSHSKVICKLCPKLHP----------MSPPIFSH 280
           WDD TS+ ++K  G+M T DE+   +F ++ V C LCP+ +P              +F+H
Sbjct: 273 WDDRTSVGLLKKDGLMATHDEETEHFFQNTDVHCVLCPR-NPDDGGSFVQDLQISTMFTH 332

Query: 281 HQKTIVVD-AQTHINAQNREIMSFIGGLDLCDGRYDTDQHSLFHTLNMESHCCDFYQTSI 340
           HQK +VVD A  + ++Q R I+SF+GGLDLCDGRYD+  HSLF TL+  +H  DF+Q + 
Sbjct: 333 HQKIVVVDSAMPNGDSQRRRIVSFVGGLDLCDGRYDSPFHSLFRTLD-SAHHDDFHQPNF 392

Query: 341 SGAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTNILLNLM--PQ 400
           +GA ++KGGPREPWHD+H+ + G  AWD+L NFEQRW KQ    LL+    L +++  P 
Sbjct: 393 AGASIEKGGPREPWHDIHSRLEGPIAWDVLFNFEQRWRKQGGKDLLIQLRELEDVIIPPS 452

Query: 401 LETNTNPQKDWNVQVFRSIDHLSASQV--------------FRNLTVERTIHEAYVEAIR 460
                +  + WNVQ+FRSID  +A                  ++  ++R+I +AY+ AIR
Sbjct: 453 PVMYPDDFEAWNVQLFRSIDGGAAFGFPETPEDAPEAGLVSGKDNIIDRSIQDAYIHAIR 512

Query: 461 RADRFIYIENQYFIGGCHLWDKD----QHCGCTNLIPIEIALKVANKIKARERFAVYIVI 520
           RA  FIYIENQYF+G    W  D    +     +LIP E++LK+ +KI A ERF VYIV+
Sbjct: 513 RAKNFIYIENQYFLGSSFGWSPDGIKPEDINALHLIPKELSLKILSKIAAGERFTVYIVV 572

Query: 521 PMWPEGPPESESVEDMLHWTRQTITMMYRLIGEAIQETGEMAHPRDYLNFFCLANREEER 580
           PMWPEG PES SV+ +L W ++T+ MMY+ I +A++  G +  PR+YL FFCL NRE ++
Sbjct: 573 PMWPEGIPESASVQAILDWQKRTMEMMYKDIVQALKANGIIEDPRNYLTFFCLGNREVKK 632

Query: 581 KWDFVPPHSPQQATQYWNAQQHRRFMVYVHSKVMIVDDLYILMGSANVNQRSMDGERDTE 640
             ++ P   P+  T Y  AQ+ RRFM+YVH+K+MIVDD YI++GSAN+NQRSMDG RD+E
Sbjct: 633 SGEYEPAEKPEPDTDYIRAQEARRFMIYVHTKMMIVDDEYIIIGSANINQRSMDGARDSE 692

Query: 641 IAIGCYQSENDGEEMPNGRDISTFRLSLWYEHTRRFEELFLDPESLQCVQRVRSIADEAW 700
           IA+G YQ  +     P    I  FR+SLWYEH    +E FL+PES +CV++V  +A++ W
Sbjct: 693 IAMGAYQPHHLSTRQPARGQIHGFRMSLWYEHLGMLDESFLNPESEECVRKVNQMAEKYW 752

Query: 701 KTYSGEEVA-DMEGVHLVTYPVKVTQEGSVEDLEENGGHFPDTKCPIKGRRSMLLPPIFT 721
             YS E +  D+ G HL+ YP+ V  EG V +L      FPDTK  + G +S  LPPI T
Sbjct: 753 DLYSSETLEHDLPG-HLLRYPIGVASEGDVTEL-PGTEFFPDTKARVLGAKSDYLPPILT 808

BLAST of Sgr016275 vs. ExPASy TrEMBL
Match: A0A6J1D3G4 (Phospholipase D OS=Momordica charantia OX=3673 GN=LOC111016958 PE=3 SV=1)

HSP 1 Score: 1397.9 bits (3617), Expect = 0.0e+00
Identity = 659/759 (86.82%), Postives = 691/759 (91.04%), Query Frame = 0

Query: 1   MEGKQKFFHGTLEVTIFHATAYTPPSPLDCIFAGGKRSYVTVKIDNKRLPR--------- 60
           MEGKQ FFHGTLEVT+FHATAYTPPSPLDC+F+GG+R+YVTVKIDNK + +         
Sbjct: 3   MEGKQMFFHGTLEVTVFHATAYTPPSPLDCLFSGGRRTYVTVKIDNKEVAQTSHEYDRVW 62

Query: 61  ------------------------------RVTTQQILNEASFINGFFPLLMENGKPSPE 120
                                          +  QQIL EASF+NGFFPL MENGKPSPE
Sbjct: 63  NQTFQILCAYPSTSTVTITMKTSRSVLGKFHIQAQQILKEASFMNGFFPLTMENGKPSPE 122

Query: 121 LRLRFMLWFKPAVYELSWKKMLGNGEYKGLRNATFHLRSNCHVTLYQDAHHLPTFRPPFH 180
           LRLRFMLWFKPAVYELSWKKMLGNGEYKGLRNATF LRSNCHVTLYQDAHHLPTF+PPFH
Sbjct: 123 LRLRFMLWFKPAVYELSWKKMLGNGEYKGLRNATFPLRSNCHVTLYQDAHHLPTFQPPFH 182

Query: 181 GSSAPRRLWEDIYKAIDNAKHLIYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQKA 240
            SS+PRRLWED+YKAIDNAKHLIYIAGWSFNPKM+LVRDSQTDIPYALGVKLGELLKQKA
Sbjct: 183 SSSSPRRLWEDVYKAIDNAKHLIYIAGWSFNPKMLLVRDSQTDIPYALGVKLGELLKQKA 242

Query: 241 EEGVAVRILIWDDETSLPIIKNAGIMKTRDEDARAYFSHSKVICKLCPKLHPMSPPIFSH 300
           EEGVAVRI+IWDD TSLPIIKNAGIMKTRDEDA AYFSHSKVIC+LCPKLHPM+PPIFSH
Sbjct: 243 EEGVAVRIMIWDDATSLPIIKNAGIMKTRDEDAWAYFSHSKVICRLCPKLHPMAPPIFSH 302

Query: 301 HQKTIVVDAQTHINAQNREIMSFIGGLDLCDGRYDTDQHSLFHTLNMESHCCDFYQTSIS 360
           HQKTI VDAQ HINA+NREIMSFIGGLDLCDGRYDT+QHSLFHTLNMESHC DFYQTSIS
Sbjct: 303 HQKTITVDAQAHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNMESHCGDFYQTSIS 362

Query: 361 GAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTNILLNLMPQLET 420
           GAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPT+ILLNLMPQLET
Sbjct: 363 GAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLNLMPQLET 422

Query: 421 NTNPQKDWNVQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRADRFIYIENQYFIGGC 480
           NTNPQ DWNVQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRA+RFIYIENQYFIGGC
Sbjct: 423 NTNPQNDWNVQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIGGC 482

Query: 481 HLWDKDQHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGPPESESVEDMLHWTR 540
           HLWDKDQHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGPPESESV +MLHWTR
Sbjct: 483 HLWDKDQHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGPPESESVAEMLHWTR 542

Query: 541 QTITMMYRLIGEAIQETGEMAHPRDYLNFFCLANREEERKWDFVPPHSPQQATQYWNAQQ 600
           QT+ MMY+LIGEAIQETGE AHPRDYLNFFCLANREEE +WDFVPPHSP++ATQYWNAQQ
Sbjct: 543 QTMKMMYKLIGEAIQETGEKAHPRDYLNFFCLANREEEGEWDFVPPHSPERATQYWNAQQ 602

Query: 601 HRRFMVYVHSKVMIVDDLYILMGSANVNQRSMDGERDTEIAIGCYQSENDGEEMPNGRDI 660
           HRRFMVYVHSKVMIVDDLYIL+GSANVNQRSMDG RDTEIAIGC+Q E D EEMPNGRDI
Sbjct: 603 HRRFMVYVHSKVMIVDDLYILIGSANVNQRSMDGGRDTEIAIGCFQQEKDAEEMPNGRDI 662

Query: 661 STFRLSLWYEHTRRFEELFLDPESLQCVQRVRSIADEAWKTYSGEEVADMEGVHLVTYPV 720
           STFRLSLWYEHTRRFEELFL+PE+L CVQRVRSIADE+W+ YSGEE ADMEGVHLV YPV
Sbjct: 663 STFRLSLWYEHTRRFEELFLNPETLDCVQRVRSIADESWRIYSGEEAADMEGVHLVAYPV 722

BLAST of Sgr016275 vs. ExPASy TrEMBL
Match: A0A5D3BW81 (Phospholipase D OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold863G001380 PE=3 SV=1)

HSP 1 Score: 1392.1 bits (3602), Expect = 0.0e+00
Identity = 653/759 (86.03%), Postives = 690/759 (90.91%), Query Frame = 0

Query: 1   MEGKQKFFHGTLEVTIFHATAYTPPSPLDCIFAGGKRSYVTVKIDNKRLPRR-------- 60
           ME KQKFFHG LEVT+FHATAYTPPSPLDC+FAGGKRSYVT+KIDNK + +         
Sbjct: 3   MEVKQKFFHGMLEVTVFHATAYTPPSPLDCLFAGGKRSYVTIKIDNKEVAQTSHEHDRVW 62

Query: 61  -------------------------------VTTQQILNEASFINGFFPLLMENGKPSPE 120
                                          +  QQIL EASFINGFFPLLMENGKPSPE
Sbjct: 63  NQTFRVLCAYPLTSTITITLKTARSVLGKFYIQAQQILKEASFINGFFPLLMENGKPSPE 122

Query: 121 LRLRFMLWFKPAVYELSWKKMLGNGEYKGLRNATFHLRSNCHVTLYQDAHHLPTFRPPFH 180
           L+LRFMLWFKPAVYELSWKKML NGEYKGLRNATF LRSNCHVTLYQDAHHLPTF+PPFH
Sbjct: 123 LKLRFMLWFKPAVYELSWKKMLANGEYKGLRNATFPLRSNCHVTLYQDAHHLPTFQPPFH 182

Query: 181 GSSAPRRLWEDIYKAIDNAKHLIYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQKA 240
           GSS PRRLWED+YKAIDNAKHL+YIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQKA
Sbjct: 183 GSSTPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQKA 242

Query: 241 EEGVAVRILIWDDETSLPIIKNAGIMKTRDEDARAYFSHSKVICKLCPKLHPMSPPIFSH 300
           EEGVAVRILIWDDETSLPIIKNAGIM T DEDARAYF HSKVIC+LCPKLHPMSPPIFSH
Sbjct: 243 EEGVAVRILIWDDETSLPIIKNAGIMNTHDEDARAYFLHSKVICRLCPKLHPMSPPIFSH 302

Query: 301 HQKTIVVDAQTHINAQNREIMSFIGGLDLCDGRYDTDQHSLFHTLNMESHCCDFYQTSIS 360
           HQK I+VDAQTHINAQNREIMSFIGGLDLCDGRYDT+QHSLFHTLN ESHC DFYQTSIS
Sbjct: 303 HQKIIIVDAQTHINAQNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCRDFYQTSIS 362

Query: 361 GAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTNILLNLMPQLET 420
           GAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPT+ILL LMPQLE+
Sbjct: 363 GAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPQLES 422

Query: 421 NTNPQKDWNVQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRADRFIYIENQYFIGGC 480
           NTNPQKDWNVQVFRSIDHLSASQ+FRN+T+ERTIHEAYVEAIRRA+RFIYIENQYFIGGC
Sbjct: 423 NTNPQKDWNVQVFRSIDHLSASQMFRNMTIERTIHEAYVEAIRRAERFIYIENQYFIGGC 482

Query: 481 HLWDKDQHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGPPESESVEDMLHWTR 540
           H WD+D+HCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEG PESESVEDMLHWTR
Sbjct: 483 HFWDRDRHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGSPESESVEDMLHWTR 542

Query: 541 QTITMMYRLIGEAIQETGEMAHPRDYLNFFCLANREEERKWDFVPPHSPQQATQYWNAQQ 600
           QT+TMMYRLIGEAIQETGE AHPRDYLNFFCLANREEERKWDF+PPHSPQ ATQYWNAQQ
Sbjct: 543 QTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFIPPHSPQHATQYWNAQQ 602

Query: 601 HRRFMVYVHSKVMIVDDLYILMGSANVNQRSMDGERDTEIAIGCYQSENDGEEMPNGRDI 660
           HRRFM+YVHSKVMIVDDLYIL+GSANVNQRSMDGERDTEIAIGCYQ ENDGEE+PNGRDI
Sbjct: 603 HRRFMIYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAIGCYQIENDGEELPNGRDI 662

Query: 661 STFRLSLWYEHTRRFEELFLDPESLQCVQRVRSIADEAWKTYSGEEVADMEGVHLVTYPV 720
           S FRLSLWYEHTR FEE+FL+PESL+CVQRVRSI D++WK YSGEEV DM+GVHLVTYPV
Sbjct: 663 SRFRLSLWYEHTRGFEEVFLNPESLKCVQRVRSIGDKSWKIYSGEEVEDMKGVHLVTYPV 722

BLAST of Sgr016275 vs. ExPASy TrEMBL
Match: A0A1S3BH90 (Phospholipase D OS=Cucumis melo OX=3656 GN=LOC103490014 PE=3 SV=1)

HSP 1 Score: 1392.1 bits (3602), Expect = 0.0e+00
Identity = 653/759 (86.03%), Postives = 690/759 (90.91%), Query Frame = 0

Query: 1   MEGKQKFFHGTLEVTIFHATAYTPPSPLDCIFAGGKRSYVTVKIDNKRLPRR-------- 60
           ME KQKFFHG LEVT+FHATAYTPPSPLDC+FAGGKRSYVT+KIDNK + +         
Sbjct: 3   MEVKQKFFHGMLEVTVFHATAYTPPSPLDCLFAGGKRSYVTIKIDNKEVAQTSHEHDRVW 62

Query: 61  -------------------------------VTTQQILNEASFINGFFPLLMENGKPSPE 120
                                          +  QQIL EASFINGFFPLLMENGKPSPE
Sbjct: 63  NQTFRVLCAYPLTSTITITLKTARSVLGKFYIQAQQILKEASFINGFFPLLMENGKPSPE 122

Query: 121 LRLRFMLWFKPAVYELSWKKMLGNGEYKGLRNATFHLRSNCHVTLYQDAHHLPTFRPPFH 180
           L+LRFMLWFKPAVYELSWKKML NGEYKGLRNATF LRSNCHVTLYQDAHHLPTF+PPFH
Sbjct: 123 LKLRFMLWFKPAVYELSWKKMLANGEYKGLRNATFPLRSNCHVTLYQDAHHLPTFQPPFH 182

Query: 181 GSSAPRRLWEDIYKAIDNAKHLIYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQKA 240
           GSS PRRLWED+YKAIDNAKHL+YIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQKA
Sbjct: 183 GSSTPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQKA 242

Query: 241 EEGVAVRILIWDDETSLPIIKNAGIMKTRDEDARAYFSHSKVICKLCPKLHPMSPPIFSH 300
           EEGVAVRILIWDDETSLPIIKNAGIM T DEDARAYF HSKVIC+LCPKLHPMSPPIFSH
Sbjct: 243 EEGVAVRILIWDDETSLPIIKNAGIMNTHDEDARAYFLHSKVICRLCPKLHPMSPPIFSH 302

Query: 301 HQKTIVVDAQTHINAQNREIMSFIGGLDLCDGRYDTDQHSLFHTLNMESHCCDFYQTSIS 360
           HQK I+VDAQTHINAQNREIMSFIGGLDLCDGRYDT+QHSLFHTLN ESHC DFYQTSIS
Sbjct: 303 HQKIIIVDAQTHINAQNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCRDFYQTSIS 362

Query: 361 GAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTNILLNLMPQLET 420
           GAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPT+ILL LMPQLE+
Sbjct: 363 GAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPQLES 422

Query: 421 NTNPQKDWNVQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRADRFIYIENQYFIGGC 480
           NTNPQKDWNVQVFRSIDHLSASQ+FRN+T+ERTIHEAYVEAIRRA+RFIYIENQYFIGGC
Sbjct: 423 NTNPQKDWNVQVFRSIDHLSASQMFRNMTIERTIHEAYVEAIRRAERFIYIENQYFIGGC 482

Query: 481 HLWDKDQHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGPPESESVEDMLHWTR 540
           H WD+D+HCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEG PESESVEDMLHWTR
Sbjct: 483 HFWDRDRHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGSPESESVEDMLHWTR 542

Query: 541 QTITMMYRLIGEAIQETGEMAHPRDYLNFFCLANREEERKWDFVPPHSPQQATQYWNAQQ 600
           QT+TMMYRLIGEAIQETGE AHPRDYLNFFCLANREEERKWDF+PPHSPQ ATQYWNAQQ
Sbjct: 543 QTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFIPPHSPQHATQYWNAQQ 602

Query: 601 HRRFMVYVHSKVMIVDDLYILMGSANVNQRSMDGERDTEIAIGCYQSENDGEEMPNGRDI 660
           HRRFM+YVHSKVMIVDDLYIL+GSANVNQRSMDGERDTEIAIGCYQ ENDGEE+PNGRDI
Sbjct: 603 HRRFMIYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAIGCYQIENDGEELPNGRDI 662

Query: 661 STFRLSLWYEHTRRFEELFLDPESLQCVQRVRSIADEAWKTYSGEEVADMEGVHLVTYPV 720
           S FRLSLWYEHTR FEE+FL+PESL+CVQRVRSI D++WK YSGEEV DM+GVHLVTYPV
Sbjct: 663 SRFRLSLWYEHTRGFEEVFLNPESLKCVQRVRSIGDKSWKIYSGEEVEDMKGVHLVTYPV 722

BLAST of Sgr016275 vs. ExPASy TrEMBL
Match: A0A6J1G9V5 (Phospholipase D OS=Cucurbita moschata OX=3662 GN=LOC111452112 PE=3 SV=1)

HSP 1 Score: 1383.2 bits (3579), Expect = 0.0e+00
Identity = 648/759 (85.38%), Postives = 693/759 (91.30%), Query Frame = 0

Query: 1   MEGKQKFFHGTLEVTIFHATAYTPPSPLDCIFAGGKRSYVTVKIDNKRLPR--------- 60
           ++GK KFFHGTLEVT+FHAT YTP SPLDCIF+GGK+SYVT+KI+N  + R         
Sbjct: 64  VQGKHKFFHGTLEVTVFHATTYTPSSPLDCIFSGGKQSYVTIKINNIEVARTNHEYDRVW 123

Query: 61  ------------------------------RVTTQQILNEASFINGFFPLLMENGKPSPE 120
                                         ++  QQIL E+SFINGFFPLLMENGKPSPE
Sbjct: 124 NQTFRVLCAHPLTSTITITMRTTRSVLGRFQIQAQQILKESSFINGFFPLLMENGKPSPE 183

Query: 121 LRLRFMLWFKPAVYELSWKKMLGNGEYKGLRNATFHLRSNCHVTLYQDAHHLPTFRPPFH 180
           LRLRFMLWF+PAVYELSWKK+L NGEYKGLRNATF LRSNCHVTLYQDAHH+ TF+PPFH
Sbjct: 184 LRLRFMLWFQPAVYELSWKKVLENGEYKGLRNATFPLRSNCHVTLYQDAHHVSTFQPPFH 243

Query: 181 GSSAPRRLWEDIYKAIDNAKHLIYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQKA 240
           GSSAPRRLWED+YKAIDNAKHL+YIAGWSFNPKMVLVRD QT IPYALGVKLGELLKQKA
Sbjct: 244 GSSAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDPQTHIPYALGVKLGELLKQKA 303

Query: 241 EEGVAVRILIWDDETSLPIIKNAGIMKTRDEDARAYFSHSKVICKLCPKLHPMSPPIFSH 300
           EEGVAVR++IWDDETSLPIIKNAGIMKT DEDARAYFS+SKV+C+LCPKLHPMSPPIFSH
Sbjct: 304 EEGVAVRLMIWDDETSLPIIKNAGIMKTHDEDARAYFSNSKVVCRLCPKLHPMSPPIFSH 363

Query: 301 HQKTIVVDAQTHINAQNREIMSFIGGLDLCDGRYDTDQHSLFHTLNMESHCCDFYQTSIS 360
           HQKTI VDAQTHINA+NREIMSFIGGLDLCDGRYDT+QHSLFHTLN ESHCCDFYQTSIS
Sbjct: 364 HQKTITVDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCCDFYQTSIS 423

Query: 361 GAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTNILLNLMPQLET 420
           GAKLQKGGPREPWHDVHA VTGEAAWDILTNFEQRWTKQSDASLLVPT+ILL LMP LE+
Sbjct: 424 GAKLQKGGPREPWHDVHARVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPPLES 483

Query: 421 NTNPQKDWNVQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRADRFIYIENQYFIGGC 480
           NTNPQKDWNVQVFRSI+HLSASQVFRNLTVERTIHEAYVEAIRRA+RFIYIENQYFIGGC
Sbjct: 484 NTNPQKDWNVQVFRSINHLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIGGC 543

Query: 481 HLWDKDQHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGPPESESVEDMLHWTR 540
           HLWDKDQHCGCTNLIPIEIALKVANKIKARE+FAVY+VIPMWPEGPPESESVEDMLHWTR
Sbjct: 544 HLWDKDQHCGCTNLIPIEIALKVANKIKAREKFAVYVVIPMWPEGPPESESVEDMLHWTR 603

Query: 541 QTITMMYRLIGEAIQETGEMAHPRDYLNFFCLANREEERKWDFVPPHSPQQATQYWNAQQ 600
           QT+TMMYRLIGEAIQETGE AHPRDYLNFFCLANREEERKWDFVPPHSPQ AT+YW+AQ 
Sbjct: 604 QTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFVPPHSPQHATEYWSAQH 663

Query: 601 HRRFMVYVHSKVMIVDDLYILMGSANVNQRSMDGERDTEIAIGCYQSENDGEEMPNGRDI 660
           HRRFMVYVHSKVMIVDDLYIL+GSANVNQRSMDGERDTEIAIGCYQ ENDG+E+PNGRDI
Sbjct: 664 HRRFMVYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAIGCYQLENDGKELPNGRDI 723

Query: 661 STFRLSLWYEHTRRFEELFLDPESLQCVQRVRSIADEAWKTYSGEEVADMEGVHLVTYPV 720
           STFRLSLWYEHT+RFEE+FL+PE+LQCV+RVRSIADE+WK YSGEEVADM+GVHLVTYPV
Sbjct: 724 STFRLSLWYEHTQRFEEVFLNPENLQCVERVRSIADESWKIYSGEEVADMKGVHLVTYPV 783

BLAST of Sgr016275 vs. ExPASy TrEMBL
Match: A0A6J1KAT1 (Phospholipase D OS=Cucurbita maxima OX=3661 GN=LOC111492613 PE=3 SV=1)

HSP 1 Score: 1376.3 bits (3561), Expect = 0.0e+00
Identity = 643/759 (84.72%), Postives = 691/759 (91.04%), Query Frame = 0

Query: 1   MEGKQKFFHGTLEVTIFHATAYTPPSPLDCIFAGGKRSYVTVKIDNKRLPR--------- 60
           ++G+  FFHGTLEVT+FHAT YTP SPLDCIF+GGK+SYVT+KI+N  + R         
Sbjct: 3   VQGEHTFFHGTLEVTVFHATTYTPSSPLDCIFSGGKQSYVTIKINNIEVARTNHEYDRVW 62

Query: 61  ------------------------------RVTTQQILNEASFINGFFPLLMENGKPSPE 120
                                         ++  QQIL E+SFINGFFPLLMENGKPSPE
Sbjct: 63  NQTFRVLCAHPLTSTITITMRTTRSVLGRFQIQAQQILKESSFINGFFPLLMENGKPSPE 122

Query: 121 LRLRFMLWFKPAVYELSWKKMLGNGEYKGLRNATFHLRSNCHVTLYQDAHHLPTFRPPFH 180
           LRLRFMLWF+PAVYELSWKK+L NGEYKGLRN TF LRSNCHVTLYQDAHH+ TF+PPFH
Sbjct: 123 LRLRFMLWFQPAVYELSWKKVLENGEYKGLRNVTFPLRSNCHVTLYQDAHHVSTFQPPFH 182

Query: 181 GSSAPRRLWEDIYKAIDNAKHLIYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQKA 240
           GSSAPRRLWED+YKAIDNAKHL+YIAGWSFNPKMVLVRD QTDIPYALGVKLGELLKQKA
Sbjct: 183 GSSAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDPQTDIPYALGVKLGELLKQKA 242

Query: 241 EEGVAVRILIWDDETSLPIIKNAGIMKTRDEDARAYFSHSKVICKLCPKLHPMSPPIFSH 300
           EEGVAVR++IWDDETSLP+IKNAGIMKT DE+ARAYFS+SKV+C+LCPKLHPMSPPIFSH
Sbjct: 243 EEGVAVRLMIWDDETSLPVIKNAGIMKTHDENARAYFSNSKVVCRLCPKLHPMSPPIFSH 302

Query: 301 HQKTIVVDAQTHINAQNREIMSFIGGLDLCDGRYDTDQHSLFHTLNMESHCCDFYQTSIS 360
           HQKTI VDAQTHINA+NREIMSFIGGLDLCDGRYDT+QHSLFHTLN ESHCCDFYQTSIS
Sbjct: 303 HQKTITVDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCCDFYQTSIS 362

Query: 361 GAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTNILLNLMPQLET 420
           GAKLQKGGPREPWHDVHA VTGEAAWDILTNFEQRWTKQSDASLLVPT+ILL LMP LE+
Sbjct: 363 GAKLQKGGPREPWHDVHARVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPPLES 422

Query: 421 NTNPQKDWNVQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRADRFIYIENQYFIGGC 480
           NTNPQKDWNVQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRA+RFIYIENQYFIGGC
Sbjct: 423 NTNPQKDWNVQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIGGC 482

Query: 481 HLWDKDQHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGPPESESVEDMLHWTR 540
           HLWDKDQHCGCTNLIPIEIALKVANKIKARE+FAVY+VIPMWPEGPPESESVEDMLHWTR
Sbjct: 483 HLWDKDQHCGCTNLIPIEIALKVANKIKAREKFAVYVVIPMWPEGPPESESVEDMLHWTR 542

Query: 541 QTITMMYRLIGEAIQETGEMAHPRDYLNFFCLANREEERKWDFVPPHSPQQATQYWNAQQ 600
           QT+TMMYRLIGEAIQETGE AHPRDYLNFFCLANREEERKWDFVPPHSPQ AT+YW+AQ 
Sbjct: 543 QTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFVPPHSPQHATEYWSAQH 602

Query: 601 HRRFMVYVHSKVMIVDDLYILMGSANVNQRSMDGERDTEIAIGCYQSENDGEEMPNGRDI 660
            RRFMVYVHSKVMIVDDLY+L+GSANVNQRSMDGERDTEIAIGCYQ ENDG+E+PNGRDI
Sbjct: 603 RRRFMVYVHSKVMIVDDLYLLIGSANVNQRSMDGERDTEIAIGCYQLENDGKELPNGRDI 662

Query: 661 STFRLSLWYEHTRRFEELFLDPESLQCVQRVRSIADEAWKTYSGEEVADMEGVHLVTYPV 720
           STFRLSLWYEHT+RFEE+FL+PE+LQCV+RVRSIADE+WK YSGEEVADM+GVHLVTYPV
Sbjct: 663 STFRLSLWYEHTQRFEEVFLNPENLQCVERVRSIADESWKIYSGEEVADMKGVHLVTYPV 722

BLAST of Sgr016275 vs. TAIR 10
Match: AT1G55180.1 (phospholipase D alpha 4 )

HSP 1 Score: 891.3 bits (2302), Expect = 5.0e-259
Identity = 424/774 (54.78%), Postives = 563/774 (72.74%), Query Frame = 0

Query: 1   MEGKQKFFHGTLEVTIFHATAYTPPSPLDCIFAGGKRSYVTVKIDNKRLPR--------- 60
           +E ++K+FHGTLE+TIF AT ++PP P +CI    K +YVT+KI+ K++ +         
Sbjct: 3   LEEQKKYFHGTLEITIFDATPFSPPFPFNCICTKPKAAYVTIKINKKKVAKTSSEYDRIW 62

Query: 61  -------------------------------RVTTQQIL-NEASFINGFFPLLMENGKPS 120
                                          R++ +QIL + ++ INGFFPL+ +NG   
Sbjct: 63  NQTFQILCAHPVTDTTITITLKTRCSVLGRFRISAEQILTSNSAVINGFFPLIADNGSTK 122

Query: 121 PELRLRFMLWFKPAVYELSWKKMLGNGEYKGLRNATFHLRSNCHVTLYQDAHHLPTFRP- 180
             L+L+ ++WF+PA  E  W + L    ++G+RNA+F  RSNC V LYQDAHH  TF P 
Sbjct: 123 RNLKLKCLMWFRPAYLEPGWCRALEEASFQGIRNASFPQRSNCRVVLYQDAHHKATFDPR 182

Query: 181 ----PFHGSSAPRRLWEDIYKAIDNAKHLIYIAGWSFNPKMVLVRDSQTDIPYALGVKLG 240
               PF+     R LWED+YKAI++A+HL+YIAGW+ NP +VLVRD++T+IP+A+GV +G
Sbjct: 183 VDDVPFNA----RNLWEDVYKAIESARHLVYIAGWALNPNLVLVRDNETEIPHAVGVTVG 242

Query: 241 ELLKQKAEEGVAVRILIWDDETSLPIIKNAGIMKTRDEDARAYFSHSKVICKLCPKLHPM 300
           ELLK+K+EEGVAVR+++W+DETSLP+IKN G+M+T  E A AYF ++ V+C+LCP+LH  
Sbjct: 243 ELLKRKSEEGVAVRVMLWNDETSLPMIKNKGVMRTNVERALAYFRNTNVVCRLCPRLHKK 302

Query: 301 SPPIFSHHQKTIVVDAQ-THINAQNREIMSFIGGLDLCDGRYDTDQHSLFHTLNMESHCC 360
            P  F+HHQKTI +D + T+ + + REIMSF+GG DLCDGRYDT++HSLF TL  E+   
Sbjct: 303 LPTAFAHHQKTITLDTRVTNSSTKEREIMSFLGGFDLCDGRYDTEEHSLFRTLGTEA--- 362

Query: 361 DFYQTSISGAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTNILL 420
           DFYQTS++GAKL +GGPREPWHD H  V G AAWD+L NFEQRWTKQ + S+LV T+ + 
Sbjct: 363 DFYQTSVAGAKLSRGGPREPWHDCHVSVVGGAAWDVLKNFEQRWTKQCNPSVLVNTSGIR 422

Query: 421 NLMPQLETNTNPQKDWNVQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRADRFIYIE 480
           NL+          + WNVQV RSIDH+SA+++ R L VE+++H+ YV AIR+A+RFIYIE
Sbjct: 423 NLVNLTGPTEENNRKWNVQVLRSIDHISATEMPRGLPVEKSVHDGYVAAIRKAERFIYIE 482

Query: 481 NQYFIGGCHLWD--KDQHC-GCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGPPES 540
           NQYF+G C  W+   D+ C GCTNLIP+EIALK+A KI+ARERFAVYIVIPMWPEGPPES
Sbjct: 483 NQYFMGSCDHWESKNDKICSGCTNLIPVEIALKIAAKIRARERFAVYIVIPMWPEGPPES 542

Query: 541 ESVEDMLHWTRQTITMMYRLIGEAIQETGEMAHPRDYLNFFCLANREEERKWDFVPPHSP 600
           E+VE++LHWTR+T++MMY++IGEAI E G+ +HPRDYLNFFCLANREE+R  +F    SP
Sbjct: 543 ETVEEILHWTRETMSMMYQIIGEAIWEVGDKSHPRDYLNFFCLANREEKRDGEFEAVSSP 602

Query: 601 QQATQYWNAQQHRRFMVYVHSKVMIVDDLYILMGSANVNQRSMDGERDTEIAIGCYQSEN 660
            Q T YWNAQ++RRFMVYVHSK+MIVDD YIL+GSAN+NQRSMDG RDTEIAIGCYQ+  
Sbjct: 603 HQKTHYWNAQRNRRFMVYVHSKLMIVDDTYILIGSANINQRSMDGCRDTEIAIGCYQTNT 662

Query: 661 DGEEMPNGRDISTFRLSLWYEHT----RRFEELFLDPESLQCVQRVRSIADEAWKTYSGE 720
           +     N  +I  +RLSLWYEHT       +    +PESL+CV+ +R+I ++ W+ YSG+
Sbjct: 663 N-----NTNEIQAYRLSLWYEHTGGKITADDLSSSEPESLECVRGLRTIGEQMWEIYSGD 722

BLAST of Sgr016275 vs. TAIR 10
Match: AT3G15730.1 (phospholipase D alpha 1 )

HSP 1 Score: 575.5 bits (1482), Expect = 6.1e-164
Identity = 298/660 (45.15%), Postives = 409/660 (61.97%), Query Frame = 0

Query: 98  SWKKMLGNGEYKGLRNATFHLRSNCHVTLYQDAHHLPTFRP--PFHGSS--APRRLWEDI 157
           +W   + + ++ G+    F  R  C V+LYQDAH    F P  P  G     P+R WEDI
Sbjct: 156 NWNMGIKSAKFPGVPYTFFSQRQGCKVSLYQDAHIPDNFVPRIPLAGGKNYEPQRCWEDI 215

Query: 158 YKAIDNAKHLIYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQKAEEGVAVRILIWD 217
           + AI NAKHLIYI GWS   ++ LVRDS+   P    V +GELLK+KA EGV V +L+WD
Sbjct: 216 FDAISNAKHLIYITGWSVYAEIALVRDSRRPKPGG-DVTIGELLKKKASEGVRVLLLVWD 275

Query: 218 DETSLPIIKNAGIMKTRDEDARAYFSHSKVICKLCPKLHP----------MSPPIFSHHQ 277
           D TS+ ++K  G+M T DE+   +F  S V C LCP+ +P              +F+HHQ
Sbjct: 276 DRTSVDVLKKDGLMATHDEETENFFRGSDVHCILCPR-NPDDGGSIVQSLQISTMFTHHQ 335

Query: 278 KTIVVDAQ--THINAQNREIMSFIGGLDLCDGRYDTDQHSLFHTLNMESHCCDFYQTSIS 337
           K +VVD++  +   ++ R I+SF+GG+DLCDGRYDT  HSLF TL+   H  DF+Q + +
Sbjct: 336 KIVVVDSEMPSRGGSEMRRIVSFVGGIDLCDGRYDTPFHSLFRTLDTVHH-DDFHQPNFT 395

Query: 338 GAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTNILLNLM--PQL 397
           GA + KGGPREPWHD+H+ + G  AWD++ NFEQRW+KQ    +LV    L +++  P  
Sbjct: 396 GAAITKGGPREPWHDIHSRLEGPIAWDVMYNFEQRWSKQGGKDILVKLRDLSDIIITPSP 455

Query: 398 ETNTNPQKDWNVQVFRSIDHLSASQV--------------FRNLTVERTIHEAYVEAIRR 457
                    WNVQ+FRSID  +A+                 ++  ++R+I +AY+ AIRR
Sbjct: 456 VMFQEDHDVWNVQLFRSIDGGAAAGFPESPEAAAEAGLVSGKDNIIDRSIQDAYIHAIRR 515

Query: 458 ADRFIYIENQYFIGGCHLWDKD----QHCGCTNLIPIEIALKVANKIKARERFAVYIVIP 517
           A  FIY+ENQYF+G    W  D    +     +LIP E++LK+ +KI+  E+F VY+V+P
Sbjct: 516 AKDFIYVENQYFLGSSFAWAADGITPEDINALHLIPKELSLKIVSKIEKGEKFRVYVVVP 575

Query: 518 MWPEGPPESESVEDMLHWTRQTITMMYRLIGEAIQETGEMAHPRDYLNFFCLANREEERK 577
           MWPEG PES SV+ +L W R+T+ MMY+ + +A++  G    PR+YL FFCL NRE ++ 
Sbjct: 576 MWPEGLPESGSVQAILDWQRRTMEMMYKDVIQALRAQGLEEDPRNYLTFFCLGNREVKKD 635

Query: 578 WDFVPPHSPQQATQYWNAQQHRRFMVYVHSKVMIVDDLYILMGSANVNQRSMDGERDTEI 637
            ++ P   P   T Y  AQ+ RRFM+YVH+K+MIVDD YI++GSAN+NQRSMDG RD+EI
Sbjct: 636 GEYEPAEKPDPDTDYMRAQEARRFMIYVHTKMMIVDDEYIIIGSANINQRSMDGARDSEI 695

Query: 638 AIGCYQSENDGEEMPNGRDISTFRLSLWYEHTRRFEELFLDPESLQCVQRVRSIADEAWK 697
           A+G YQ  +     P    I  FR+SLWYEH    +E FLDP SL+C+++V  I+D+ W 
Sbjct: 696 AMGGYQPHHLSHRQPARGQIHGFRMSLWYEHLGMLDETFLDPSSLECIEKVNRISDKYWD 755

Query: 698 TYSGEEVA-DMEGVHLVTYPVKVTQEGSVEDLEENGGHFPDTKCPIKGRRSMLLPPIFTT 721
            YS E +  D+ G HL+ YP+ V  EG + +L      FPDTK  I G +S  LPPI TT
Sbjct: 756 FYSSESLEHDLPG-HLLRYPIGVASEGDITEL-PGFEFFPDTKARILGTKSDYLPPILTT 810

BLAST of Sgr016275 vs. TAIR 10
Match: AT1G52570.1 (phospholipase D alpha 2 )

HSP 1 Score: 574.3 bits (1479), Expect = 1.4e-163
Identity = 301/660 (45.61%), Postives = 414/660 (62.73%), Query Frame = 0

Query: 98  SWKKMLGNGEYKGLRNATFHLRSNCHVTLYQDAHHLPTFRP--PFHGSS--APRRLWEDI 157
           +W + + + ++ G+    F  R  C V+LYQDAH    F P  P  G     P R WEDI
Sbjct: 156 NWNRGIKSAKFPGVPYTFFSQRRGCKVSLYQDAHIPGNFVPKIPLAGGKNYEPHRCWEDI 215

Query: 158 YKAIDNAKHLIYIAGWSFNPKMVLVRDSQTDIPYALG-VKLGELLKQKAEEGVAVRILIW 217
           + AI NAKHLIYI GWS   ++ LVRDS+   P   G V +GELLK+KA EGV V +L+W
Sbjct: 216 FDAITNAKHLIYITGWSVYTEISLVRDSRR--PKQGGDVTVGELLKKKASEGVKVILLVW 275

Query: 218 DDETSLPIIKNAGIMKTRDEDARAYFSHSKVICKLCPK---------LHPMSPPIFSHHQ 277
           DD TS+ ++K  G+M T DE+   +F  + V C LCP+          +     +F+HHQ
Sbjct: 276 DDRTSVDLLKKDGLMATHDEETENFFRGTDVNCILCPRNPDDGGSIVQNLQISTMFTHHQ 335

Query: 278 KTIVVDAQTHI-NAQNREIMSFIGGLDLCDGRYDTDQHSLFHTLNMESHCCDFYQTSISG 337
           K +VVD++     +++R I+SF+GGLDLCDGRYDT  HSLF TL+  +H  DF+Q + +G
Sbjct: 336 KIVVVDSEMPSGGSRSRRIVSFVGGLDLCDGRYDTPFHSLFRTLD-TAHHDDFHQPNFTG 395

Query: 338 AKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTNILLNLM--PQLE 397
           A + KGGPREPWHD+H  + G  AWD+L NFEQRW++Q    +LV    L +++  P   
Sbjct: 396 AAITKGGPREPWHDIHCRLEGPIAWDVLYNFEQRWSRQGGKDILVKMRELGDIIIPPSPV 455

Query: 398 TNTNPQKDWNVQVFRSIDHLSASQV--------------FRNLTVERTIHEAYVEAIRRA 457
             +     WNVQ+FRSID  +A+                 ++  ++R+I +AY+ AIRRA
Sbjct: 456 LFSEDHDVWNVQLFRSIDGGAAAGFPDSPEAAAEAGLVSGKDNIIDRSIQDAYIHAIRRA 515

Query: 458 DRFIYIENQYFIGGCHLWDKD----QHCGCTNLIPIEIALKVANKIKARERFAVYIVIPM 517
             FIYIENQYF+G    W  D    +     +LIP E++LK+ +KIKA E+F VY+V+PM
Sbjct: 516 KDFIYIENQYFLGSSFAWSADGIKPEEINALHLIPKELSLKIVSKIKAGEKFKVYVVVPM 575

Query: 518 WPEGPPESESVEDMLHWTRQTITMMYRLIGEAIQETG-EMAHPRDYLNFFCLANREEERK 577
           WPEG PES SV+ +L W ++T+ MMY+ + +A++E G E   PRDYL FFCL NRE ++ 
Sbjct: 576 WPEGIPESGSVQAILDWQKRTMEMMYKDVIKALRENGLEGEDPRDYLTFFCLGNREVKKD 635

Query: 578 WDFVPPHSPQQATQYWNAQQHRRFMVYVHSKVMIVDDLYILMGSANVNQRSMDGERDTEI 637
            ++ P   P+  T Y  AQ+ RRFM+YVH+K+MIVDD YI++GSAN+NQRSMDG RD+EI
Sbjct: 636 GEYEPSEKPEPDTDYIRAQEARRFMIYVHTKMMIVDDEYIIIGSANINQRSMDGARDSEI 695

Query: 638 AIGCYQSENDGEEMPNGRDISTFRLSLWYEHTRRFEELFLDPESLQCVQRVRSIADEAWK 697
           A+G YQ  +     P    I  FR+SLWYEH    +E FLDP S +C+Q+V  +AD+ W 
Sbjct: 696 AMGGYQPYHLSTRQPARGQIHGFRMSLWYEHLGMLDETFLDPSSQECIQKVNRVADKYWD 755

Query: 698 TYSGEEVA-DMEGVHLVTYPVKVTQEGSVEDLEENGGHFPDTKCPIKGRRSMLLPPIFTT 721
            YS E +  D+ G HL+ YP+ +  EG++ +L      FPDTK  I G +S  +PPI TT
Sbjct: 756 LYSSESLEHDLPG-HLLRYPIGIASEGNITEL-PGCEFFPDTKARILGVKSDYMPPILTT 810

BLAST of Sgr016275 vs. TAIR 10
Match: AT5G25370.1 (phospholipase D alpha 3 )

HSP 1 Score: 573.2 bits (1476), Expect = 3.0e-163
Identity = 314/699 (44.92%), Postives = 419/699 (59.94%), Query Frame = 0

Query: 65  INGFFPLLMENGKP-SPELRLRFMLWFKPAVYELSWKKMLGNGEYKGLRNATFHLRSNCH 124
           I+ +  +L EN +P     +L   + F     +++W K +    + G+ NA F+ R  C 
Sbjct: 129 IDRWLDILDENRRPIQGGSKLHVRVKFTHVTQDVNWNKGIILPSFNGVPNAYFNQREGCK 188

Query: 125 VTLYQDAHHLPTFRP-PFHGSSA---PRRLWEDIYKAIDNAKHLIYIAGWSFNPKMVLVR 184
           VTLYQDAH L  +      G        R WE+I+ AI  AKHLIYIAGWS N  + LVR
Sbjct: 189 VTLYQDAHVLNEYPDVTLTGGQVIYKHHRCWEEIFDAIWEAKHLIYIAGWSVNTDVTLVR 248

Query: 185 DSQTDIPYALGVKLGELLKQKAEEGVAVRILIWDDETSLPIIKNAGIMKTRDEDARAYFS 244
           D +   P    +KLGELLK+KAEE V V +L+WDD TS  + K  G+M T D++   YF 
Sbjct: 249 DPKRTRPGG-DLKLGELLKKKAEENVTVLMLVWDDRTSHEVFKRDGLMMTHDQETYDYFK 308

Query: 245 HSKVICKLCPKLHPMSPPI---------FSHHQKTIVVDAQTHINAQNREIMSFIGGLDL 304
           ++KV C LCP+       I         F+HHQKTIVVD++   +   R I+SF+GG+DL
Sbjct: 309 NTKVRCVLCPRNPDNGDSIVQGFEVATMFTHHQKTIVVDSEVDGSLTKRRIVSFLGGIDL 368

Query: 305 CDGRYDTDQHSLFHTLNMESHCCDFYQTSISGAKLQKGGPREPWHDVHACVTGEAAWDIL 364
           CDGRYDT +H LF TLN   H  DF+Q +  GA ++KGGPREPWHD+H  + G AAWD+L
Sbjct: 369 CDGRYDTVEHPLFGTLN-SVHANDFHQPNFDGASIKKGGPREPWHDIHCKLDGPAAWDVL 428

Query: 365 TNFEQRWTKQSDA-----SLLVPTNILLNLMPQLETNTNPQKDWNVQVFRSIDHLSASQV 424
            NFEQRW KQ        S+     I +  +P ++ +   ++ W VQVFRSID   A + 
Sbjct: 429 YNFEQRWMKQGSGRRYLISMAQLAEITVPPLPIVQPDN--EEGWTVQVFRSIDD-GAVEG 488

Query: 425 F---------------RNLTVERTIHEAYVEAIRRADRFIYIENQYFIGGCHLWDKD--- 484
           F               ++  +ER+I +AYV AIRRA  FIYIENQYF+G    W+     
Sbjct: 489 FPEDPREAASIGLISGKDNVIERSIQDAYVNAIRRAKNFIYIENQYFLGSSFGWNSRDIN 548

Query: 485 -QHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGPPESESVEDMLHWTRQTITM 544
                   LIP EI+LK+ +KI+A ERF+VYIVIP+WPEG P S SV+ +L W R+T+ M
Sbjct: 549 LNEINALQLIPKEISLKIVSKIEAGERFSVYIVIPLWPEGKPGSASVQAILDWQRRTMEM 608

Query: 545 MYRLIGEAIQETGEMAHPRDYLNFFCLANREEERKWDFVPPHSPQQATQYWNAQQHRRFM 604
           MY  I  A+++ G  A+PRDYL FFCL NRE+ +  +++PP  P+  + Y  AQ+ RRFM
Sbjct: 609 MYTDIIIALRKKGLDANPRDYLTFFCLGNREKGKVGEYLPPEKPEANSDYARAQESRRFM 668

Query: 605 VYVHSKVMIVDDLYILMGSANVNQRSMDGERDTEIAIGCYQSEN--DGEEMPNGRDISTF 664
           +YVHSK+MIVDD YI++GSAN+NQRSMDG RDTEIA+G YQ  +      M     I +F
Sbjct: 669 IYVHSKMMIVDDEYIIIGSANINQRSMDGGRDTEIAMGAYQPSHLLSTNNMRPVGQIFSF 728

Query: 665 RLSLWYEHTRRFEELFLDPESLQCVQRVRSIADEAWKTYSGEEVA---DMEGVHLVTYPV 721
           R+SLW EH R     F  PES +C++ V + ADE W  YS +E     D+ G HL++YP+
Sbjct: 729 RISLWLEHLRVTTNAFQCPESEECIRMVNATADELWGLYSAQEYPRNDDLPG-HLLSYPI 788

BLAST of Sgr016275 vs. TAIR 10
Match: AT2G42010.1 (phospholipase D beta 1 )

HSP 1 Score: 520.0 bits (1338), Expect = 3.0e-147
Identity = 304/706 (43.06%), Postives = 407/706 (57.65%), Query Frame = 0

Query: 53   VTTQQILNEASFINGFFPLLMENGKP-SPELRLRFMLWFKPAVYELSWKKMLGNG-EYKG 112
            +  +QI + A  I G +P+L  NGKP  P   L   + + P      +   +G G +Y+G
Sbjct: 378  IPVEQIYSGAK-IEGTYPILNSNGKPCKPGANLSLSIQYTPMDKLSVYHHGVGAGPDYQG 437

Query: 113  LRNATFHLRSNCHVTLYQDAH----HLPTFRPPFHGSSAPRRLWEDIYKAIDNAKHLIYI 172
            +    F LR    V LYQDAH     LP  R     S    + W D++ AI  A+ LIYI
Sbjct: 438  VPGTYFPLRKGGTVRLYQDAHVPEGMLPGIRLDNGMSYEHGKCWHDMFDAIRQARRLIYI 497

Query: 173  AGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQKAEEGVAVRILIWDDETSLPII--KNA 232
             GWS   K+ L+RD    +  A    LGELL+ K++EGV V +LIWDD TS  I+  K  
Sbjct: 498  TGWSVWHKVKLIRDK---LGPASECTLGELLRSKSQEGVRVLLLIWDDPTSRSILGYKTD 557

Query: 233  GIMKTRDEDARAYFSHSKVICKLCP----KLHPMSP-----PIFSHHQKTIVVDAQTHIN 292
            G+M T DE+ R +F HS V   LCP    K H          I++HHQK ++VDA     
Sbjct: 558  GVMATHDEETRRFFKHSSVQVLLCPRNAGKRHSWVKQREVGTIYTHHQKNVIVDADA--G 617

Query: 293  AQNREIMSFIGGLDLCDGRYDTDQHSLFHTLNMESHCCDFYQTSISGAKLQKGGPREPWH 352
               R+I++F+GGLDLCDGRYDT QH LF TL    H  DF+  + +G     G PREPWH
Sbjct: 618  GNRRKIIAFVGGLDLCDGRYDTPQHPLFRTL-QTIHKDDFHNPTFTGN--LSGCPREPWH 677

Query: 353  DVHACVTGEAAWDILTNFEQRWTKQS------------DASLLVPTNI--LLNLMPQLET 412
            D+H+ + G AA+D+LTNFE+RW K +            D +LL    I  +L +      
Sbjct: 678  DLHSKIDGPAAYDVLTNFEERWLKAAKPSGIKKFKTSYDDALLRIDRIPDILGVSDTPTV 737

Query: 413  NTNPQKDWNVQVFRSIDHLSAS--------------QVFRNLTVERTIHEAYVEAIRRAD 472
            + N  + W+VQ+FRSID  S                   +N+ ++ +IH AYV+AIR A 
Sbjct: 738  SENDPEAWHVQIFRSIDSNSVKGFPKDPKDATCKNLVCGKNVLIDMSIHTAYVKAIRAAQ 797

Query: 473  RFIYIENQYFIGGCHLWDKDQHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGP 532
             FIYIENQYFIG  + W+  +  G  NLIP+EIALK+A KI+A ERFA YIVIPMWPEG 
Sbjct: 798  HFIYIENQYFIGSSYNWNAHKDIGANNLIPMEIALKIAEKIRANERFAAYIVIPMWPEGV 857

Query: 533  PESESVEDMLHWTRQTITMMYRLIGEAIQETG--EMAHPRDYLNFFCLANREEERKWDFV 592
            P   + + +L+W  +TI MMY  I +A+ ETG      P+DYLNFFCL NRE     D  
Sbjct: 858  PTGAATQRILYWQHKTIQMMYETIYKALVETGLEGAFSPQDYLNFFCLGNREMVDGIDNS 917

Query: 593  PPHSPQQA-TQYWNAQQHRRFMVYVHSKVMIVDDLYILMGSANVNQRSMDGERDTEIAIG 652
               SP  A T    +++ RRFMVYVHSK M+VDD Y+++GSAN+NQRSM+G RDTEIA+G
Sbjct: 918  GTGSPSNANTPQALSRKSRRFMVYVHSKGMVVDDEYVVIGSANINQRSMEGTRDTEIAMG 977

Query: 653  CYQSENDGEEMPNG--RDISTFRLSLWYEHTRRFEELFLDPESLQCVQRVRSIADEAWKT 709
             YQ ++      +G    I  +R+SLW EH    ++ F  PES++CV++VR++ +  WK 
Sbjct: 978  AYQPQHTWARKHSGPRGQIYGYRMSLWAEHMATLDDCFTQPESIECVRKVRTMGERNWKQ 1037

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022148254.10.0e+0086.82phospholipase D alpha 4 [Momordica charantia][more]
XP_038879261.10.0e+0086.03phospholipase D alpha 4 [Benincasa hispida][more]
XP_008447602.10.0e+0086.03PREDICTED: phospholipase D alpha 4 [Cucumis melo] >KAA0051141.1 phospholipase D ... [more]
XP_004146826.10.0e+0085.51phospholipase D alpha 4 [Cucumis sativus] >KAE8651293.1 hypothetical protein Csa... [more]
XP_023525777.10.0e+0085.38phospholipase D alpha 4 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9C8887.0e-25854.78Phospholipase D alpha 4 OS=Arabidopsis thaliana OX=3702 GN=PLDALPHA4 PE=2 SV=1[more]
Q432709.4e-17044.01Phospholipase D alpha 1 OS=Zea mays OX=4577 GN=PLD1 PE=2 SV=1[more]
Q430072.7e-16944.29Phospholipase D alpha 1 OS=Oryza sativa subsp. japonica OX=39947 GN=PLD1 PE=1 SV... [more]
P934001.8e-16543.61Phospholipase D alpha 1 OS=Nicotiana tabacum OX=4097 GN=PLD1 PE=2 SV=2[more]
Q411423.1e-16543.55Phospholipase D alpha 1 OS=Ricinus communis OX=3988 GN=PLD1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1D3G40.0e+0086.82Phospholipase D OS=Momordica charantia OX=3673 GN=LOC111016958 PE=3 SV=1[more]
A0A5D3BW810.0e+0086.03Phospholipase D OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold863G0013... [more]
A0A1S3BH900.0e+0086.03Phospholipase D OS=Cucumis melo OX=3656 GN=LOC103490014 PE=3 SV=1[more]
A0A6J1G9V50.0e+0085.38Phospholipase D OS=Cucurbita moschata OX=3662 GN=LOC111452112 PE=3 SV=1[more]
A0A6J1KAT10.0e+0084.72Phospholipase D OS=Cucurbita maxima OX=3661 GN=LOC111492613 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G55180.15.0e-25954.78phospholipase D alpha 4 [more]
AT3G15730.16.1e-16445.15phospholipase D alpha 1 [more]
AT1G52570.11.4e-16345.61phospholipase D alpha 2 [more]
AT5G25370.13.0e-16344.92phospholipase D alpha 3 [more]
AT2G42010.13.0e-14743.06phospholipase D beta 1 [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001736Phospholipase D/TransphosphatidylaseSMARTSM00155pld_4coord: 259..294
e-value: 15.0
score: 11.9
coord: 565..592
e-value: 3.2E-4
score: 30.0
IPR001736Phospholipase D/TransphosphatidylasePFAMPF00614PLDccoord: 259..294
e-value: 1.5E-7
score: 31.3
IPR001736Phospholipase D/TransphosphatidylasePROSITEPS50035PLDcoord: 565..592
score: 13.869999
IPR001736Phospholipase D/TransphosphatidylasePROSITEPS50035PLDcoord: 257..294
score: 9.572399
IPR024632Phospholipase D, C-terminalPFAMPF12357PLD_Ccoord: 637..709
e-value: 8.5E-25
score: 86.4
NoneNo IPR availableGENE3D3.30.870.10Endonuclease Chain Acoord: 404..610
e-value: 6.1E-11
score: 44.1
NoneNo IPR availableGENE3D3.30.870.10Endonuclease Chain Acoord: 135..390
e-value: 3.0E-26
score: 93.8
NoneNo IPR availablePANTHERPTHR18896:SF137PHOSPHOLIPASE D ALPHA 4coord: 53..720
NoneNo IPR availablePANTHERPTHR18896:SF137PHOSPHOLIPASE D ALPHA 4coord: 5..51
NoneNo IPR availableSUPERFAMILY56024Phospholipase D/nucleasecoord: 105..367
NoneNo IPR availableSUPERFAMILY56024Phospholipase D/nucleasecoord: 382..649
IPR011402Phospholipase D, plantPIRSFPIRSF036470PLD_plantcoord: 2..49
e-value: 49.0
score: -0.6
coord: 44..720
e-value: 6.6E-257
score: 852.4
IPR015679Phospholipase D familyPANTHERPTHR18896PHOSPHOLIPASE Dcoord: 5..51
IPR015679Phospholipase D familyPANTHERPTHR18896PHOSPHOLIPASE Dcoord: 53..720

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr016275.1Sgr016275.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046470 phosphatidylcholine metabolic process
biological_process GO:0009395 phospholipid catabolic process
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0070290 N-acylphosphatidylethanolamine-specific phospholipase D activity
molecular_function GO:0004630 phospholipase D activity
molecular_function GO:0003824 catalytic activity