Sgr016241 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr016241
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description1-aminocyclopropane-1-carboxylate oxidase
Locationtig00007935: 231954 .. 233539 (-)
RNA-Seq ExpressionSgr016241
SyntenySgr016241
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCGCCTGAACCCACCCCGGCGAACCCCTTCGACTTCCGGGCGCCGCCGCCCTCCCCCATAGGCACTGGCCGCCGCTCCTGCGTCACAAACGAAGACGTTCTCACCGAGTTCCTCGAGCACTCTCTTCGGGTCCCGGATCTCGTCTTGCCCGACAACATTTTCCCGAGGCAGAAGTTTATCGAGAACCCGCCCAGAATTGATCTCAGGTCGCTTTGTTCTTCCCAGAGTGATTCTGTTTTGAAGGTTTTTTTGGATTCCATGACCAGCATTGGGTGTTTCCAGCTGGTGAACCATGGGGTTCCGGCGGAGCTTATAGGGTCGGTTTTGGCTTCGGCTGCCGCCGGGGTTTTCTGCCTCTCGCCGGAGAAGAAGGTGGCGGTGACGAGATCGCCGGAGAAGGCGTATGGGTTTGAGGAGTATTGGCATGGAGAGGATGAGAGTGAACTAAGTGAAGAGTTTGTATGGAGCAGAGACGAAGGCTTGAAGCTGGAAATGGAGGCGATTTCGCCATCTGGATATTCTAATTTCAGGTAATTTTATCAACAACCTAAGCTTTTCTTTTATTGTTCAAATATTTCTCATCAATTTAATATTCAAATTAATCTTATTTGAAATATGACAATTTTTTTCCTAAAATATATTCTCTAAATTATATCTTCTTTTTAATTTTGTCTGCCTTAAAAGTTCTAATTTCGTCCAAAAATCTAAAAAGTTTCAATAAAATCCAACTCTACTAAACTTATGGTGGTATAACATTTAAAAAATATTATTTATATGATACAACATTTTTTACATGAGAAATGTTTATTCACCTCTATTCGATTTAAAAAATTCAACCAACACAAGTCAAATTAAAAACTCAATTGGTAAGGTATATTATCATAGACCTATATATCTTACCCAATTAAAAAAAATTATCACCTGATTAAGATATTATCCCAAATATTTTAAATGTCGCATCAGTCATCATCTTGAAGGAATTTGATTGAAATATTACAAAAATTAGAGATATATATGTGTGTGTCTATATATATATATATATATATATCCAATGACAATCACATAAAGATTTGCAAAGTGGCATTTAAAAAAAAAAAAACCATTGAAAAATATGTTAGTTTAACATGAACCCACAATACAAAACTGAATTTTGTCGCAATACAATTTGATCCCAATCTCTTTGTTCCTTCTCTTACATTTTCACATGGCAGCAAGAAAATGGAAGCGCTGACTGAAGTAACAGAGAAAATGGGGAGAAAATGTTGGAAATTTTCCGGGAAAATTGTGGCCAGAAAATTGGAGAAAAGGAAGGGGTGGAGGCGCATGGATCAGTGTGGTGTGTGTACAAGCAGAGGCAGAGAGTGGGTTGGGATGATGAGTGGGAAAACTGTTTGAAATATGATGTGATCAGAATGCTTTTAAGGGGAACTGATTTCTCTCATGCTTTCTCCTTCCATTTCTCCCATGGATCTTCTTCTCTCTTTCATGTTTACTCCAAGAGAGGCTGGGTTTCTTTCGTACCCGATGAAGCACCATTCTCGTCACTGTCGGAGATCAAATTCAGGTACTCAAACTGTTTTTAG

mRNA sequence

ATGTCGCCTGAACCCACCCCGGCGAACCCCTTCGACTTCCGGGCGCCGCCGCCCTCCCCCATAGGCACTGGCCGCCGCTCCTGCGTCACAAACGAAGACGTTCTCACCGAGTTCCTCGAGCACTCTCTTCGGGTCCCGGATCTCGTCTTGCCCGACAACATTTTCCCGAGGCAGAAGTTTATCGAGAACCCGCCCAGAATTGATCTCAGGTCGCTTTGTTCTTCCCAGAGTGATTCTGTTTTGAAGGTTTTTTTGGATTCCATGACCAGCATTGGGTGTTTCCAGCTGGTGAACCATGGGGTTCCGGCGGAGCTTATAGGGTCGGTTTTGGCTTCGGCTGCCGCCGGGGTTTTCTGCCTCTCGCCGGAGAAGAAGGTGGCGGTGACGAGATCGCCGGAGAAGGCGTATGGGTTTGAGGAGTATTGGCATGGAGAGGATGAGAGTGAACTAAGTGAAGAGTTTGTATGGAGCAGAGACGAAGGCTTGAAGCTGGAAATGGAGGCGATTTCGCCATCTGGATATTCTAATTTCAGAGAAAATGGGGAGAAAATGTTGGAAATTTTCCGGGAAAATTGTGGCCAGAAAATTGGAGAAAAGGAAGGGGTGGAGGCGCATGGATCAGTGTGGTGTGTGTACAAGCAGAGGCAGAGAGTGGGTTGGGATGATGAGTGGGAAAACTGTTTGAAATATGATGTGATCAGAATGCTTTTAAGGGGAACTGATTTCTCTCATGCTTTCTCCTTCCATTTCTCCCATGGATCTTCTTCTCTCTTTCATGTTTACTCCAAGAGAGGCTGGGTTTCTTTCGTACCCGATGAAGCACCATTCTCGTCACTGTCGGAGATCAAATTCAGGTACTCAAACTGTTTTTAG

Coding sequence (CDS)

ATGTCGCCTGAACCCACCCCGGCGAACCCCTTCGACTTCCGGGCGCCGCCGCCCTCCCCCATAGGCACTGGCCGCCGCTCCTGCGTCACAAACGAAGACGTTCTCACCGAGTTCCTCGAGCACTCTCTTCGGGTCCCGGATCTCGTCTTGCCCGACAACATTTTCCCGAGGCAGAAGTTTATCGAGAACCCGCCCAGAATTGATCTCAGGTCGCTTTGTTCTTCCCAGAGTGATTCTGTTTTGAAGGTTTTTTTGGATTCCATGACCAGCATTGGGTGTTTCCAGCTGGTGAACCATGGGGTTCCGGCGGAGCTTATAGGGTCGGTTTTGGCTTCGGCTGCCGCCGGGGTTTTCTGCCTCTCGCCGGAGAAGAAGGTGGCGGTGACGAGATCGCCGGAGAAGGCGTATGGGTTTGAGGAGTATTGGCATGGAGAGGATGAGAGTGAACTAAGTGAAGAGTTTGTATGGAGCAGAGACGAAGGCTTGAAGCTGGAAATGGAGGCGATTTCGCCATCTGGATATTCTAATTTCAGAGAAAATGGGGAGAAAATGTTGGAAATTTTCCGGGAAAATTGTGGCCAGAAAATTGGAGAAAAGGAAGGGGTGGAGGCGCATGGATCAGTGTGGTGTGTGTACAAGCAGAGGCAGAGAGTGGGTTGGGATGATGAGTGGGAAAACTGTTTGAAATATGATGTGATCAGAATGCTTTTAAGGGGAACTGATTTCTCTCATGCTTTCTCCTTCCATTTCTCCCATGGATCTTCTTCTCTCTTTCATGTTTACTCCAAGAGAGGCTGGGTTTCTTTCGTACCCGATGAAGCACCATTCTCGTCACTGTCGGAGATCAAATTCAGGTACTCAAACTGTTTTTAG

Protein sequence

MSPEPTPANPFDFRAPPPSPIGTGRRSCVTNEDVLTEFLEHSLRVPDLVLPDNIFPRQKFIENPPRIDLRSLCSSQSDSVLKVFLDSMTSIGCFQLVNHGVPAELIGSVLASAAAGVFCLSPEKKVAVTRSPEKAYGFEEYWHGEDESELSEEFVWSRDEGLKLEMEAISPSGYSNFRENGEKMLEIFRENCGQKIGEKEGVEAHGSVWCVYKQRQRVGWDDEWENCLKYDVIRMLLRGTDFSHAFSFHFSHGSSSLFHVYSKRGWVSFVPDEAPFSSLSEIKFRYSNCF
Homology
BLAST of Sgr016241 vs. NCBI nr
Match: XP_038882380.1 (uncharacterized protein LOC120073645 [Benincasa hispida])

HSP 1 Score: 422.5 bits (1085), Expect = 2.8e-114
Identity = 211/286 (73.78%), Postives = 239/286 (83.57%), Query Frame = 0

Query: 1   MSPEPTPANPFDFRAPPPSPIGTGRRSCVTNEDVLTEFLEHSLRVPDLVLPDNIFPRQKF 60
           MSPE +   PF+FRAPPPSPIGT RRS VTN++VLTEFLEHSLRVPDLVLP+NIFPRQ+F
Sbjct: 2   MSPERSAVTPFEFRAPPPSPIGTSRRSSVTNDEVLTEFLEHSLRVPDLVLPENIFPRQRF 61

Query: 61  IENPPRIDLRSLCSSQSDSVLKVFLDSMTSIGCFQLVNHGVPAELIGSVLASAAAGVFCL 120
           IENPPRID R + SS  DSVLK+ LDSM SIG FQLVNHG+PA+LIG+V A+AAAGVF +
Sbjct: 62  IENPPRIDFRMIESSDRDSVLKI-LDSMASIGFFQLVNHGIPAQLIGAVAAAAAAGVFGI 121

Query: 121 SPEKKVAVTRSPEKAYGFEEYWHGEDESELSEEFVWSRDEGLKLEMEAISPSGYSNFREN 180
           SPEKKVAV RSPEKAYGFEEYWHGEDESEL EEFVWSRDEGLK+EMEAISP GYS+F + 
Sbjct: 122 SPEKKVAVARSPEKAYGFEEYWHGEDESELCEEFVWSRDEGLKVEMEAISPFGYSSFSKK 181

Query: 181 -----------GEKMLEIFRENCGQKIGEKEGVEAHGSVWCVYKQ-RQRVGWDDEWENCL 240
                      GEK+LEI  EN G K+ +K+ +  HGSVWCVYKQ +QR+ W+DE ENC 
Sbjct: 182 MEWMTQLTEKVGEKILEILVENSGGKVEKKDVILGHGSVWCVYKQKKQRMNWNDELENCF 241

Query: 241 KYDVIRMLLRGTDFSHAFSFHFSHGSSSLFHVYSKRGWVSFVPDEA 275
           K+DVIRML+RGTDFSHAF FHF HGSS LFHVY+KRGWVSFVPDE+
Sbjct: 242 KHDVIRMLIRGTDFSHAFCFHFCHGSSPLFHVYTKRGWVSFVPDES 286

BLAST of Sgr016241 vs. NCBI nr
Match: KAA0050830.1 (1-aminocyclopropane-1-carboxylate oxidase [Cucumis melo var. makuwa])

HSP 1 Score: 397.9 bits (1021), Expect = 7.5e-107
Identity = 205/288 (71.18%), Postives = 229/288 (79.51%), Query Frame = 0

Query: 1   MSPEPTPANPFDFRAPPPSPIGTGRRSCVTNEDVLTEFLEHSLRVPDLVLPDNIFPRQKF 60
           MSPE   A PF+FRAPPPSPIGT RRS VTN++VLTEFLEHSLRVPDLVLP+ IFPR++F
Sbjct: 4   MSPERAAATPFEFRAPPPSPIGTSRRSSVTNDEVLTEFLEHSLRVPDLVLPETIFPRERF 63

Query: 61  IENPPRIDLRSLCSSQSDSVLKVFLDSMTSIGCFQLVNHGVPAELIGSVLASAAAGVFCL 120
           +ENPPRID R + S   DSVLK+ LDSM S G FQLVNHG+P ELIG+V A+A AGVF +
Sbjct: 64  VENPPRIDFRLIESYDRDSVLKI-LDSMRSFGFFQLVNHGIPVELIGAVAAAADAGVFGI 123

Query: 121 SPEKKVAVTRSPEKAYGFEEYWHGEDESELSEEFVWSRDEGLKLEMEAISPSGYSNFREN 180
           SPEKKVAV RSPEKAYGFEE WHGEDESE+SEEFVW+RDE LK+EMEAISP GYSNF + 
Sbjct: 124 SPEKKVAVGRSPEKAYGFEELWHGEDESEVSEEFVWNRDEDLKMEMEAISPIGYSNFSKK 183

Query: 181 -----------GEKMLEIFRENCGQKIGEKEGVEAHGSVWCVYKQ--RQRVG-WDDEWEN 240
                      GEK+LEIF EN G KI   E    HGSVWCVYKQ  +QR+  W+DE EN
Sbjct: 184 MEWLTQLTEKLGEKILEIFLENSG-KIARNEVFSGHGSVWCVYKQKIKQRLSVWNDELEN 243

Query: 241 CLKYDVIRMLLRGTDFSHAFSFHFSHGSSSLFHVYSKRGWVSFVPDEA 275
           C K+DVIRML+RGTDFSHAF FHF HGSS LFH YSKRGWVSFV DE+
Sbjct: 244 CFKHDVIRMLIRGTDFSHAFCFHFCHGSSPLFHAYSKRGWVSFVADES 289

BLAST of Sgr016241 vs. NCBI nr
Match: TYK08517.1 (1-aminocyclopropane-1-carboxylate oxidase [Cucumis melo var. makuwa])

HSP 1 Score: 397.5 bits (1020), Expect = 9.8e-107
Identity = 205/288 (71.18%), Postives = 228/288 (79.17%), Query Frame = 0

Query: 1   MSPEPTPANPFDFRAPPPSPIGTGRRSCVTNEDVLTEFLEHSLRVPDLVLPDNIFPRQKF 60
           MSPE   A PF+FRAPPPSPIGT RRS VTN++VLTEFLEHSLRVPDLVLP+ IFPR++F
Sbjct: 4   MSPERAAATPFEFRAPPPSPIGTSRRSSVTNDEVLTEFLEHSLRVPDLVLPETIFPRERF 63

Query: 61  IENPPRIDLRSLCSSQSDSVLKVFLDSMTSIGCFQLVNHGVPAELIGSVLASAAAGVFCL 120
           +ENPPRID R + S   DSVLK+ LDSM S G FQLVNHG+P ELIG+V A+A AGVF +
Sbjct: 64  VENPPRIDFRLIESYDRDSVLKI-LDSMRSFGFFQLVNHGIPVELIGAVAAAADAGVFGI 123

Query: 121 SPEKKVAVTRSPEKAYGFEEYWHGEDESELSEEFVWSRDEGLKLEMEAISPSGYSNFREN 180
           SPEKKVAV RSPEKAYGFEE WHGEDESE+SEEFVW+RDE LK+EMEAISP GYSNF + 
Sbjct: 124 SPEKKVAVGRSPEKAYGFEELWHGEDESEVSEEFVWNRDEDLKMEMEAISPIGYSNFSKK 183

Query: 181 -----------GEKMLEIFRENCGQKIGEKEGVEAHGSVWCVYKQ--RQRVG-WDDEWEN 240
                      GEKMLEIF EN G KI   E    HG VWCVYKQ  +QR+  W+DE EN
Sbjct: 184 MEWLTQLTEKLGEKMLEIFLENSG-KIARNEVFSGHGPVWCVYKQKIKQRLSVWNDELEN 243

Query: 241 CLKYDVIRMLLRGTDFSHAFSFHFSHGSSSLFHVYSKRGWVSFVPDEA 275
           C K+DVIRML+RGTDFSHAF FHF HGSS LFH YSKRGWVSFV DE+
Sbjct: 244 CFKHDVIRMLIRGTDFSHAFCFHFCHGSSPLFHAYSKRGWVSFVADES 289

BLAST of Sgr016241 vs. NCBI nr
Match: XP_004151051.2 (uncharacterized protein LOC101203918 [Cucumis sativus] >KGN58037.1 hypothetical protein Csa_010946 [Cucumis sativus])

HSP 1 Score: 394.4 bits (1012), Expect = 8.3e-106
Identity = 202/288 (70.14%), Postives = 225/288 (78.12%), Query Frame = 0

Query: 1   MSPEPTPANPFDFRAPPPSPIGTGRRSCVTNEDVLTEFLEHSLRVPDLVLPDNIFPRQKF 60
           MSPE   A PF+FRAPPPSPI T RRS VTN++VLTEFLEHSLRVPDLVLPD IFPR++F
Sbjct: 4   MSPERAAATPFEFRAPPPSPIRTSRRSSVTNDEVLTEFLEHSLRVPDLVLPDTIFPRERF 63

Query: 61  IENPPRIDLRSLCSSQSDSVLKVFLDSMTSIGCFQLVNHGVPAELIGSVLASAAAGVFCL 120
           +E+PPRID R + S   DSVLK+ LDSM S G FQLVNHG+P ELIG+V  +A AGVF +
Sbjct: 64  VEHPPRIDYRLIESYDRDSVLKI-LDSMCSFGFFQLVNHGIPVELIGAVAGAADAGVFGI 123

Query: 121 SPEKKVAVTRSPEKAYGFEEYWHGEDESELSEEFVWSRDEGLKLEMEAISPSGYSNFREN 180
           SPEKKV V RSPEKAYGFEEYWHGEDESE+SEEFVWSRDE LK+EME ISP GYSNF + 
Sbjct: 124 SPEKKVGVGRSPEKAYGFEEYWHGEDESEVSEEFVWSRDEDLKMEMEVISPIGYSNFSKK 183

Query: 181 -----------GEKMLEIFRENCGQKIGEKEGVEAHGSVWCVYKQRQRVG---WDDEWEN 240
                      GEKML IF EN G KI   E V  HGSVWCVYK + + G   W+DE EN
Sbjct: 184 MEWLTQLTEKLGEKMLGIFLENSG-KIARNEVVLGHGSVWCVYKHKIKQGLSVWNDELEN 243

Query: 241 CLKYDVIRMLLRGTDFSHAFSFHFSHGSSSLFHVYSKRGWVSFVPDEA 275
           C K+DVIRML+RGTDFSHAF FHF HGSS LFH YSKRGWVSFVPD++
Sbjct: 244 CFKHDVIRMLIRGTDFSHAFCFHFCHGSSPLFHAYSKRGWVSFVPDDS 289

BLAST of Sgr016241 vs. NCBI nr
Match: XP_022948619.1 (uncharacterized protein LOC111452244 [Cucurbita moschata])

HSP 1 Score: 384.4 bits (986), Expect = 8.6e-103
Identity = 199/290 (68.62%), Postives = 230/290 (79.31%), Query Frame = 0

Query: 1   MSPEPTPANPFDFRAPPPSPIGTGRRSCVTNEDVLTEFLEHSLRVPDLVLPDNIFPRQKF 60
           MSPE T A PFDFRAPPPSPI T RRS VTN+DVLT+FLEHSLRVP+LVLP+NIFPRQ+F
Sbjct: 4   MSPELTAATPFDFRAPPPSPINTSRRSSVTNDDVLTDFLEHSLRVPNLVLPENIFPRQRF 63

Query: 61  IENPPRIDLRSLCSSQSDSVLKVFLDSMTSIGCFQLVNHGVPAELIGSVLASAAAGVFCL 120
           I+NPPRID RS+ SS  DSV+++ LDSM SIGCFQL NHG+P ELIG+V ++AAAGVF +
Sbjct: 64  IQNPPRIDFRSIESSDHDSVVRI-LDSMGSIGCFQLGNHGIPMELIGAVASAAAAGVFGI 123

Query: 121 SPEKKVAVTRSPEKAYGFEEYWHGEDESELSEEFVWSRDEGLKLEMEAISPSGYSNFREN 180
           S EKK  VTRSPEK YGFEEYW GEDESE+SEEFVWSRDEGL+ EME IS  GYS+F   
Sbjct: 124 SREKKTVVTRSPEKGYGFEEYWDGEDESEVSEEFVWSRDEGLRKEMEGISGFGYSDFSNK 183

Query: 181 -----------GEKMLEIFRENCGQKIGEKEGVEAHGSVWCVYKQRQRVGWDDEWENCLK 240
                      GEK+LEIFRENCG K+ EKE     GSVWCV+KQ+QR   +DE ENCLK
Sbjct: 184 MEALTQVTEKVGEKILEIFRENCG-KVAEKEVGWRQGSVWCVHKQKQR--QNDELENCLK 243

Query: 241 YDVIRMLLRGTDFSHAFSFHFSHGSSS-----LFHVYSKRGWVSFVPDEA 275
           +DV+RML+RG+D SHA  FHF HGSSS     +FHVYSK+GWV FVP+E+
Sbjct: 244 HDVMRMLIRGSDLSHALCFHFCHGSSSSSSSCVFHVYSKKGWVCFVPEES 289

BLAST of Sgr016241 vs. ExPASy Swiss-Prot
Match: Q08508 (1-aminocyclopropane-1-carboxylate oxidase 4 OS=Petunia hybrida OX=4102 GN=ACO4 PE=3 SV=1)

HSP 1 Score: 46.2 bits (108), Expect = 7.3e-04
Identity = 19/49 (38.78%), Postives = 35/49 (71.43%), Query Frame = 0

Query: 61  IENPPRIDLRSLCSSQSDSVLKVFLDSMTSIGCFQLVNHGVPAELIGSV 110
           +EN P I+L +LC ++ D+ +++  D+  + G F+LVNHG+P E++ +V
Sbjct: 1   MENFPIINLENLCGAERDATMEMIKDACENWGFFELVNHGIPHEVMDTV 49

BLAST of Sgr016241 vs. ExPASy TrEMBL
Match: A0A5A7U6N0 (1-aminocyclopropane-1-carboxylate oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold404G00900 PE=4 SV=1)

HSP 1 Score: 397.9 bits (1021), Expect = 3.6e-107
Identity = 205/288 (71.18%), Postives = 229/288 (79.51%), Query Frame = 0

Query: 1   MSPEPTPANPFDFRAPPPSPIGTGRRSCVTNEDVLTEFLEHSLRVPDLVLPDNIFPRQKF 60
           MSPE   A PF+FRAPPPSPIGT RRS VTN++VLTEFLEHSLRVPDLVLP+ IFPR++F
Sbjct: 4   MSPERAAATPFEFRAPPPSPIGTSRRSSVTNDEVLTEFLEHSLRVPDLVLPETIFPRERF 63

Query: 61  IENPPRIDLRSLCSSQSDSVLKVFLDSMTSIGCFQLVNHGVPAELIGSVLASAAAGVFCL 120
           +ENPPRID R + S   DSVLK+ LDSM S G FQLVNHG+P ELIG+V A+A AGVF +
Sbjct: 64  VENPPRIDFRLIESYDRDSVLKI-LDSMRSFGFFQLVNHGIPVELIGAVAAAADAGVFGI 123

Query: 121 SPEKKVAVTRSPEKAYGFEEYWHGEDESELSEEFVWSRDEGLKLEMEAISPSGYSNFREN 180
           SPEKKVAV RSPEKAYGFEE WHGEDESE+SEEFVW+RDE LK+EMEAISP GYSNF + 
Sbjct: 124 SPEKKVAVGRSPEKAYGFEELWHGEDESEVSEEFVWNRDEDLKMEMEAISPIGYSNFSKK 183

Query: 181 -----------GEKMLEIFRENCGQKIGEKEGVEAHGSVWCVYKQ--RQRVG-WDDEWEN 240
                      GEK+LEIF EN G KI   E    HGSVWCVYKQ  +QR+  W+DE EN
Sbjct: 184 MEWLTQLTEKLGEKILEIFLENSG-KIARNEVFSGHGSVWCVYKQKIKQRLSVWNDELEN 243

Query: 241 CLKYDVIRMLLRGTDFSHAFSFHFSHGSSSLFHVYSKRGWVSFVPDEA 275
           C K+DVIRML+RGTDFSHAF FHF HGSS LFH YSKRGWVSFV DE+
Sbjct: 244 CFKHDVIRMLIRGTDFSHAFCFHFCHGSSPLFHAYSKRGWVSFVADES 289

BLAST of Sgr016241 vs. ExPASy TrEMBL
Match: A0A5D3C991 (1-aminocyclopropane-1-carboxylate oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold323G00540 PE=4 SV=1)

HSP 1 Score: 397.5 bits (1020), Expect = 4.7e-107
Identity = 205/288 (71.18%), Postives = 228/288 (79.17%), Query Frame = 0

Query: 1   MSPEPTPANPFDFRAPPPSPIGTGRRSCVTNEDVLTEFLEHSLRVPDLVLPDNIFPRQKF 60
           MSPE   A PF+FRAPPPSPIGT RRS VTN++VLTEFLEHSLRVPDLVLP+ IFPR++F
Sbjct: 4   MSPERAAATPFEFRAPPPSPIGTSRRSSVTNDEVLTEFLEHSLRVPDLVLPETIFPRERF 63

Query: 61  IENPPRIDLRSLCSSQSDSVLKVFLDSMTSIGCFQLVNHGVPAELIGSVLASAAAGVFCL 120
           +ENPPRID R + S   DSVLK+ LDSM S G FQLVNHG+P ELIG+V A+A AGVF +
Sbjct: 64  VENPPRIDFRLIESYDRDSVLKI-LDSMRSFGFFQLVNHGIPVELIGAVAAAADAGVFGI 123

Query: 121 SPEKKVAVTRSPEKAYGFEEYWHGEDESELSEEFVWSRDEGLKLEMEAISPSGYSNFREN 180
           SPEKKVAV RSPEKAYGFEE WHGEDESE+SEEFVW+RDE LK+EMEAISP GYSNF + 
Sbjct: 124 SPEKKVAVGRSPEKAYGFEELWHGEDESEVSEEFVWNRDEDLKMEMEAISPIGYSNFSKK 183

Query: 181 -----------GEKMLEIFRENCGQKIGEKEGVEAHGSVWCVYKQ--RQRVG-WDDEWEN 240
                      GEKMLEIF EN G KI   E    HG VWCVYKQ  +QR+  W+DE EN
Sbjct: 184 MEWLTQLTEKLGEKMLEIFLENSG-KIARNEVFSGHGPVWCVYKQKIKQRLSVWNDELEN 243

Query: 241 CLKYDVIRMLLRGTDFSHAFSFHFSHGSSSLFHVYSKRGWVSFVPDEA 275
           C K+DVIRML+RGTDFSHAF FHF HGSS LFH YSKRGWVSFV DE+
Sbjct: 244 CFKHDVIRMLIRGTDFSHAFCFHFCHGSSPLFHAYSKRGWVSFVADES 289

BLAST of Sgr016241 vs. ExPASy TrEMBL
Match: A0A0A0LBM9 (DIOX_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G454120 PE=4 SV=1)

HSP 1 Score: 394.4 bits (1012), Expect = 4.0e-106
Identity = 202/288 (70.14%), Postives = 225/288 (78.12%), Query Frame = 0

Query: 1   MSPEPTPANPFDFRAPPPSPIGTGRRSCVTNEDVLTEFLEHSLRVPDLVLPDNIFPRQKF 60
           MSPE   A PF+FRAPPPSPI T RRS VTN++VLTEFLEHSLRVPDLVLPD IFPR++F
Sbjct: 4   MSPERAAATPFEFRAPPPSPIRTSRRSSVTNDEVLTEFLEHSLRVPDLVLPDTIFPRERF 63

Query: 61  IENPPRIDLRSLCSSQSDSVLKVFLDSMTSIGCFQLVNHGVPAELIGSVLASAAAGVFCL 120
           +E+PPRID R + S   DSVLK+ LDSM S G FQLVNHG+P ELIG+V  +A AGVF +
Sbjct: 64  VEHPPRIDYRLIESYDRDSVLKI-LDSMCSFGFFQLVNHGIPVELIGAVAGAADAGVFGI 123

Query: 121 SPEKKVAVTRSPEKAYGFEEYWHGEDESELSEEFVWSRDEGLKLEMEAISPSGYSNFREN 180
           SPEKKV V RSPEKAYGFEEYWHGEDESE+SEEFVWSRDE LK+EME ISP GYSNF + 
Sbjct: 124 SPEKKVGVGRSPEKAYGFEEYWHGEDESEVSEEFVWSRDEDLKMEMEVISPIGYSNFSKK 183

Query: 181 -----------GEKMLEIFRENCGQKIGEKEGVEAHGSVWCVYKQRQRVG---WDDEWEN 240
                      GEKML IF EN G KI   E V  HGSVWCVYK + + G   W+DE EN
Sbjct: 184 MEWLTQLTEKLGEKMLGIFLENSG-KIARNEVVLGHGSVWCVYKHKIKQGLSVWNDELEN 243

Query: 241 CLKYDVIRMLLRGTDFSHAFSFHFSHGSSSLFHVYSKRGWVSFVPDEA 275
           C K+DVIRML+RGTDFSHAF FHF HGSS LFH YSKRGWVSFVPD++
Sbjct: 244 CFKHDVIRMLIRGTDFSHAFCFHFCHGSSPLFHAYSKRGWVSFVPDDS 289

BLAST of Sgr016241 vs. ExPASy TrEMBL
Match: A0A6J1GAF1 (uncharacterized protein LOC111452244 OS=Cucurbita moschata OX=3662 GN=LOC111452244 PE=4 SV=1)

HSP 1 Score: 384.4 bits (986), Expect = 4.2e-103
Identity = 199/290 (68.62%), Postives = 230/290 (79.31%), Query Frame = 0

Query: 1   MSPEPTPANPFDFRAPPPSPIGTGRRSCVTNEDVLTEFLEHSLRVPDLVLPDNIFPRQKF 60
           MSPE T A PFDFRAPPPSPI T RRS VTN+DVLT+FLEHSLRVP+LVLP+NIFPRQ+F
Sbjct: 4   MSPELTAATPFDFRAPPPSPINTSRRSSVTNDDVLTDFLEHSLRVPNLVLPENIFPRQRF 63

Query: 61  IENPPRIDLRSLCSSQSDSVLKVFLDSMTSIGCFQLVNHGVPAELIGSVLASAAAGVFCL 120
           I+NPPRID RS+ SS  DSV+++ LDSM SIGCFQL NHG+P ELIG+V ++AAAGVF +
Sbjct: 64  IQNPPRIDFRSIESSDHDSVVRI-LDSMGSIGCFQLGNHGIPMELIGAVASAAAAGVFGI 123

Query: 121 SPEKKVAVTRSPEKAYGFEEYWHGEDESELSEEFVWSRDEGLKLEMEAISPSGYSNFREN 180
           S EKK  VTRSPEK YGFEEYW GEDESE+SEEFVWSRDEGL+ EME IS  GYS+F   
Sbjct: 124 SREKKTVVTRSPEKGYGFEEYWDGEDESEVSEEFVWSRDEGLRKEMEGISGFGYSDFSNK 183

Query: 181 -----------GEKMLEIFRENCGQKIGEKEGVEAHGSVWCVYKQRQRVGWDDEWENCLK 240
                      GEK+LEIFRENCG K+ EKE     GSVWCV+KQ+QR   +DE ENCLK
Sbjct: 184 MEALTQVTEKVGEKILEIFRENCG-KVAEKEVGWRQGSVWCVHKQKQR--QNDELENCLK 243

Query: 241 YDVIRMLLRGTDFSHAFSFHFSHGSSS-----LFHVYSKRGWVSFVPDEA 275
           +DV+RML+RG+D SHA  FHF HGSSS     +FHVYSK+GWV FVP+E+
Sbjct: 244 HDVMRMLIRGSDLSHALCFHFCHGSSSSSSSCVFHVYSKKGWVCFVPEES 289

BLAST of Sgr016241 vs. ExPASy TrEMBL
Match: A0A6J1K8P0 (uncharacterized protein LOC111492693 OS=Cucurbita maxima OX=3661 GN=LOC111492693 PE=4 SV=1)

HSP 1 Score: 373.6 bits (958), Expect = 7.3e-100
Identity = 193/287 (67.25%), Postives = 226/287 (78.75%), Query Frame = 0

Query: 1   MSPEPTPANPFDFRAPPPSPIGTGRRSCVTNEDVLTEFLEHSLRVPDLVLPDNIFPRQKF 60
           MSPE T A PFDFRAPPPSPI T RRS VTN+DVLT+FLEHSLRVP+LVLP+NIFPRQ+F
Sbjct: 4   MSPEHTAAIPFDFRAPPPSPINTSRRSSVTNDDVLTDFLEHSLRVPNLVLPENIFPRQRF 63

Query: 61  IENPPRIDLRSLCSSQSDSVLKVFLDSMTSIGCFQLVNHGVPAELIGSVLASAAAGVFCL 120
           I+ PPRID RS+ SS  DSV+++ LDSM SIGCFQL NHG+P ELIG+  ++AA GVF +
Sbjct: 64  IQTPPRIDFRSIESSDHDSVVRI-LDSMGSIGCFQLGNHGIPTELIGAAASAAAVGVFGI 123

Query: 121 SPEKKVAVTRSPEKAYGFEEYWHGEDESELSEEFVWSRDEGLKLEMEAISPSGYSNF--- 180
           S EKK+ VTRSPEK YGFEEYW GEDESE+SEEFVWSRDEGL+ EME IS  GYS+F   
Sbjct: 124 SREKKMVVTRSPEKGYGFEEYWDGEDESEVSEEFVWSRDEGLRKEMEGISGFGYSDFSNK 183

Query: 181 --------RENGEKMLEIFRENCGQKIGEKEGVEAHGSVWCVYKQRQRVGWDDEWENCLK 240
                    + GEK+++IFRENCG K+ EKE     GSVWCVYKQ+Q    +DE E CLK
Sbjct: 184 MEALTQVTEKMGEKIVKIFRENCG-KVAEKEVGSGQGSVWCVYKQKQ----NDELEKCLK 243

Query: 241 YDVIRMLLRGTDFSHAFSFHFSHGSSS--LFHVYSKRGWVSFVPDEA 275
           +DV+RML+RG+D SHA  FHF HGSSS  +FHV SKRGWV FVP+E+
Sbjct: 244 HDVMRMLIRGSDLSHALCFHFWHGSSSSCVFHVCSKRGWVCFVPEES 284

BLAST of Sgr016241 vs. TAIR 10
Match: AT1G79760.1 (downstream target of AGL15-4 )

HSP 1 Score: 137.9 bits (346), Expect = 1.3e-32
Identity = 101/265 (38.11%), Postives = 132/265 (49.81%), Query Frame = 0

Query: 12  DFRAPPPSPIGTGRRSCVT-NEDVLTEFLEHSLRVPDLVLPDNIFPRQKFIENPPRIDLR 71
           DFRAPPPSP+ + RR+  T NEDVL+EFL+   RVP+LVLPD +FP+  F+ NPP  D  
Sbjct: 7   DFRAPPPSPVASSRRASFTANEDVLSEFLDKCGRVPNLVLPDKVFPKHTFLLNPPTFDFH 66

Query: 72  SLCSSQSDSVLKVFLDSMTSIGCFQLVNHGVPAELIGSVLASAAAGVFCLSPEKKVAVTR 131
            L S      L   LD++ +IGCFQLVNHGVP  ++                        
Sbjct: 67  RLDS------LSALLDAIATIGCFQLVNHGVPEAMV------------------------ 126

Query: 132 SPEKAYGFEEYWHGEDESELSEEFVWSRDEGLKLEMEAISPSGYSNFRE---NGEKMLEI 191
              KA   +  +H E     +EEFV+++D              YS+ RE     E++ + 
Sbjct: 127 ---KAAMDKTIFHDE-----TEEFVFNKDINADDHTAC-----YSDLRELMGEAERIGKA 186

Query: 192 FRENCGQ-KIGEKEGVEAHGSVWCVYKQRQRVGWDDEWENCLKYDVIRMLLRGTDFSHAF 251
            RE  G     +KEGV         Y +R  +      +N  K + IRMLLRG D  H+ 
Sbjct: 187 VREKLGAGGRSQKEGVGV------CYVKRHNI------KNESKEEAIRMLLRGYDERHSL 213

Query: 252 SFHFSHGSSSLFHVYSKRGWVSFVP 272
             +F H     FHVYSKRGWVSF P
Sbjct: 247 CLNFCHAE---FHVYSKRGWVSFSP 213

BLAST of Sgr016241 vs. TAIR 10
Match: AT2G38500.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 53.9 bits (128), Expect = 2.5e-07
Identity = 53/188 (28.19%), Postives = 83/188 (44.15%), Query Frame = 0

Query: 16  PPPSPIGTGRRSCVTNEDVLTEFLEHSLRVPDLVLPDNIFPRQKFIEN---PPRIDLRSL 75
           PPPSPI   R S     ++LTE +E S++VP+L LP++    +        P  ID R L
Sbjct: 20  PPPSPIPRARGSRCAASEILTEIIERSIQVPELTLPESHSGGESCGSRHLIPAEIDFRLL 79

Query: 76  CSSQSDSVLKVFLDSMTSIGCFQLVNHGVPAELIGSVLASAAAGVFCLSPEKKVAVTRSP 135
            S +  SV ++ + S    G F++  HG+  E + S L   +  VF +   +        
Sbjct: 80  ASRREGSVDRL-VRSAREFGAFRVSYHGISGEELRS-LVRESGRVFGVLEGRDT------ 139

Query: 136 EKAYGFEEYWHGEDESELSEEFVWSRD--EGLKLEMEAISPSGYSNFRENGEKMLEIFRE 195
               GF     G       +E VW R   E ++   E I P  Y  F +  E + +   E
Sbjct: 140 ----GFHRSVVGN-----RDEIVWVRSWKERMEWAREYIGPERYRCFSQEMENVADKL-E 189

Query: 196 NCGQKIGE 199
           +  +K+G+
Sbjct: 200 DIARKLGQ 189

BLAST of Sgr016241 vs. TAIR 10
Match: AT3G12900.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 43.9 bits (102), Expect = 2.6e-04
Identity = 28/72 (38.89%), Postives = 41/72 (56.94%), Query Frame = 0

Query: 56  PRQKFI--ENPPRIDLRSLCSSQSDSVLKVFLDSMTSIGCFQLVNHGVPAELIGSVLASA 115
           P QK +  E    IDL +L   Q   V K  +++  ++G FQ+VNHGV  EL+  +L S+
Sbjct: 46  PTQKALTCEATQPIDLSNLDGPQHKEVAKQIVEAAETLGFFQVVNHGVSVELL-ELLKSS 105

Query: 116 AAGVFCLSPEKK 126
           A   F  +PE+K
Sbjct: 106 AHEFFAQAPEEK 116

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038882380.12.8e-11473.78uncharacterized protein LOC120073645 [Benincasa hispida][more]
KAA0050830.17.5e-10771.181-aminocyclopropane-1-carboxylate oxidase [Cucumis melo var. makuwa][more]
TYK08517.19.8e-10771.181-aminocyclopropane-1-carboxylate oxidase [Cucumis melo var. makuwa][more]
XP_004151051.28.3e-10670.14uncharacterized protein LOC101203918 [Cucumis sativus] >KGN58037.1 hypothetical ... [more]
XP_022948619.18.6e-10368.62uncharacterized protein LOC111452244 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q085087.3e-0438.781-aminocyclopropane-1-carboxylate oxidase 4 OS=Petunia hybrida OX=4102 GN=ACO4 P... [more]
Match NameE-valueIdentityDescription
A0A5A7U6N03.6e-10771.181-aminocyclopropane-1-carboxylate oxidase OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A5D3C9914.7e-10771.181-aminocyclopropane-1-carboxylate oxidase OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A0A0LBM94.0e-10670.14DIOX_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G454120 PE=4... [more]
A0A6J1GAF14.2e-10368.62uncharacterized protein LOC111452244 OS=Cucurbita moschata OX=3662 GN=LOC1114522... [more]
A0A6J1K8P07.3e-10067.25uncharacterized protein LOC111492693 OS=Cucurbita maxima OX=3661 GN=LOC111492693... [more]
Match NameE-valueIdentityDescription
AT1G79760.11.3e-3238.11downstream target of AGL15-4 [more]
AT2G38500.12.5e-0728.192-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT3G12900.12.6e-0438.892-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 65..141
e-value: 8.1E-10
score: 39.5
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 62..197
e-value: 5.0E-13
score: 51.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..24
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..17
NoneNo IPR availablePANTHERPTHR479902-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATEDcoord: 8..272
NoneNo IPR availablePANTHERPTHR47990:SF126SUBFAMILY NOT NAMEDcoord: 8..272
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 65..186

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr016241.1Sgr016241.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane