Sgr016159 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr016159 vs. NCBI nr
Match: RXH80310.1 (hypothetical protein DVH24_041457 [Malus domestica])

HSP 1 Score: 1261.5 bits (3263), Expect = 0.0e+00
Identity = 801/1520 (52.70%), Postives = 934/1520 (61.45%), Query Frame = 0

Query: 1    MREQDSGVANFGLPEEVLEVLPSDPFEQLDVARKITSIALSTRVSALESETSALHAQLSD 60
            M  ++SG +NF LPEEVLEVLP DPFEQLDVARKITS+ALSTRVSALESE+SAL  +L++
Sbjct: 1    MLAKESGSSNFDLPEEVLEVLPPDPFEQLDVARKITSLALSTRVSALESESSALREKLAE 60

Query: 61   KDVLISDLQSQIESLDAALSEKSGKLARADQEKETLLQENASLSNTVKKLTRDVSKLEVF 120
            KD LI+DLQSQ+ESLDA+LSE + KLA A+QEKE L++E A L+NTV+KL+RDVSKLEVF
Sbjct: 61   KDRLIADLQSQVESLDASLSESADKLALANQEKEGLVKEKAVLANTVRKLSRDVSKLEVF 120

Query: 121  RKTLMQSLHE-EENSTGGSEAAAK----LESQESLPSAPHIGDD------LTLPPSRHSS 180
            RKTLM+SL E EEN +G S+  AK    ++++E   S PH GD        TLPPSR SS
Sbjct: 121  RKTLMRSLAEDEENPSGASDVVAKPANTVQAEEHSSSKPHDGDGEGDVTFSTLPPSRSSS 180

Query: 181  VQINTSETVNSLKEDHDTDASRPRISNSLLLASQTSTPRLTPPGSPPISSASVSPTRTSK 240
            VQ +T    ++  ED DTDASRPRI++SLLLASQTSTPR TPPGSPPI SAS SPTRTSK
Sbjct: 181  VQ-STFSGGSTFTEDRDTDASRPRIAHSLLLASQTSTPRFTPPGSPPIYSASTSPTRTSK 240

Query: 241  LFPQSVIQCHSLSQGGCLIGPRCIRLCSLAIAVQLQALMQEGLLLVLFPHLKWFLKKILK 300
                                P   +  S++ A      M +    V   H          
Sbjct: 241  --------------------PGSPKRHSMSFATSRG--MFDNRSSVPSSH---------- 300

Query: 301  RGDEDVNVVLFRYKELLEWFLFDGARRTRVDGKEFFRQVRSRLSYEQFSAFLTNVKELNA 360
             G E                    + RTRVDGKEFFRQVRSRLSYEQF AFL NVKELN 
Sbjct: 301  HGSE--------------------SGRTRVDGKEFFRQVRSRLSYEQFGAFLANVKELNG 360

Query: 361  HKQTKENDVELEGRCKFKSDFGASNFGGQSKIERMLKWRREFMPIATHLSLSVILLPSFM 420
            HKQTKE                     G S++                            
Sbjct: 361  HKQTKEI--------------------GYSQV---------------------------- 420

Query: 421  ENDQETSASSSSSKACERTLLRSKMLEISIIEKLDHRDSADMAGCDPIELDKSGSNDSDD 480
             ND+E   +SS          R  ML                       L    SN S +
Sbjct: 421  HNDKEWLFTSSQ---------RQGML-----------------------LKLHFSNGSRE 480

Query: 481  IASENQSNDQINQVVGTPNVGGRRAR-GFSDSFSTQELRLLDLENEAK-DESSPRGVLED 540
             A ENQ   +      + + G RRAR GFS+SFS+Q+LR L+L+ +    E S RGV ED
Sbjct: 481  TAKENQ---EAQATTPSAHGGSRRARAGFSESFSSQDLRSLNLDEDVSVHELSSRGVSED 540

Query: 541  --CSRSLDSETTTSRASTSDWEGH--------PDSRNPSHWRGFFRLLKKGPQMRFQTFP 600
              C RSL S  ++   STSD +G          D R    WRGFFR+LKKGPQ  F TFP
Sbjct: 541  EYCPRSLGSGASSPMTSTSDSDGRGSRTATSDSDPRANYQWRGFFRILKKGPQYPFPTFP 600

Query: 601  PL-KTVPKLTRRKSKRIREDMLPVLSPVMNSSIETEFCYFRSSWKNFTLSELQAACNNFS 660
            P     PKLTRRKSK+I  D +P ++     S + +FC F+SSWKN++L EL+AA NNFS
Sbjct: 601  PKGAQKPKLTRRKSKKI--DFIPQINSPTLRSFDADFCRFKSSWKNYSLLELEAATNNFS 660

Query: 661  QENLIGEGGYSEVYKGQLVDGQLVAIKRLTRGSQEEMTADFLSELGIIVHVDHPNIASVI 720
             ENLIGEGGY+EVYKG L DGQ+VAIKRLTRGSQEEMTADFLSELG+IVHVDHPNIA +I
Sbjct: 661  HENLIGEGGYAEVYKGTLEDGQIVAIKRLTRGSQEEMTADFLSELGVIVHVDHPNIAKLI 720

Query: 721  GYGVEGGMHLILHLSPHGSLASILYGSKERLDWTTRYKIALGIAEGLLYLHEGCQRRIIH 780
            GYGVEGGMHL+LHLSPHGSL+SILYG +E LDW  RYK+A G A+GLLYLHEGCQRRIIH
Sbjct: 721  GYGVEGGMHLVLHLSPHGSLSSILYGPRENLDWDIRYKVAFGTAKGLLYLHEGCQRRIIH 780

Query: 781  RDIKASNILLRDDFEPQISDFGLAKWLPDQWTHHTVSKFEGTFGYLPPEFFMHGIVDEKT 840
            +DIKASNILL +DFEPQISDFGLAKWLPD WTHH VSKFEGTFGYLPPEFFMHGIVDEKT
Sbjct: 781  KDIKASNILLAEDFEPQISDFGLAKWLPDSWTHHVVSKFEGTFGYLPPEFFMHGIVDEKT 840

Query: 841  DVYAYGVLLLELITGRRALDSSQQSLVMWAKPLLSKHDIKELVDPSLVDAYDIGQMNRMI 900
            DVYAYGVLLLELITGR+ALDSS +SLVMW                               
Sbjct: 841  DVYAYGVLLLELITGRQALDSSHKSLVMW------------------------------- 900

Query: 901  SVASMCIHQSSIQRPEMSQVVQILKGDEVGLE-LVKRQKSKLQRTYSEELLDAEEYNSTK 960
                               +VQIL+GDE  LE   K  KSKLQRTYSEELLDA+EYNSTK
Sbjct: 901  -------------------IVQILEGDETSLEYATKYHKSKLQRTYSEELLDADEYNSTK 960

Query: 961  YLNDLDRHLEACKPKLRFLLANHSFAIREEAPLRNSGFAEFDIFSDKSLSLSLSLSLSLS 1020
            YL+D D+ LE       F+L   +   R                    + L L L L   
Sbjct: 961  YLSDRDKQLE-------FILGTSTLMTR--------------------VCLHLLLLLLFF 1020

Query: 1021 TLIRARLCPDFALCYLSSCFRSHRLFGNPLVRMLVRQAPLAFVYEGFLTDLECDHLISLA 1080
             L    +C   +    SS   S     NP     +   P AFVYEG LTD ECDHLIS+A
Sbjct: 1021 LL---SICSTSS----SSSSASRTFTVNPSKVKQISWNPRAFVYEGLLTDAECDHLISIA 1080

Query: 1081 RSELKRSEVADNESGKSKLSTVRTSSGMFISKSKDPIVAGIEDKIAAWTFLPKENGEDMQ 1140
            +SELKRS VADN SG+SKLS VRTSSGMFI K+KDPIVAGIEDK++ WTFLPKENGED+Q
Sbjct: 1081 KSELKRSAVADNLSGQSKLSEVRTSSGMFIPKAKDPIVAGIEDKLSTWTFLPKENGEDIQ 1140

Query: 1141 VLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSDVTKGGETVFPWQRHDNIRQLI 1200
            VLRYE GQKYE HYDYFVDKVNIA GGHR+ATVLMYL+DV KGGETVFP           
Sbjct: 1141 VLRYEPGQKYEPHYDYFVDKVNIARGGHRIATVLMYLTDVAKGGETVFP----------- 1200

Query: 1201 CLTLLYNFCEQKFP-HRRASETDEDLSECARKGIAVKPRK--VMPYFSL----------- 1260
                     E + P  R+A+E D  LSECA+KGIAVKP++   + +FSL           
Sbjct: 1201 ---------EAEIPLRRKAAEIDHSLSECAKKGIAVKPQRGDALLFFSLTPHAVPDPNSL 1260

Query: 1261 ------VLNQMLSQTPRVCME----------------------VALSLKEKNGQQQSGFT 1320
                  +  +  S T  + ++                       AL    KN +   G  
Sbjct: 1261 HAGCPVIEGEKWSATKWIHVDSFDKNLDASGDCGDLNESCERWAALGECTKNQEYMVGTP 1278

Query: 1321 EAAGFQSSE----------PERQKGKRSKSNHLSLSAS----------MEVGLMQRQWIE 1380
            E  G+               + Q   ++    LS S S          MEVG MQRQW++
Sbjct: 1321 ELPGYCRRSCKSLICAIIPQDLQFKAQAFLCWLSFSYSVCVERNKRKDMEVGQMQRQWVD 1278

Query: 1381 YTKSLFMEGFLDSQFIELQKLQDEGNPDFIVEVVSLFFEDSERLLNDLTRALDEPDVDFQ 1434
            YTKSLF+EGFLD QF++LQ+LQDE NPDF+VEVVSLFFEDSE+LLNDLTRAL++P VDF+
Sbjct: 1381 YTKSLFLEGFLDGQFLQLQQLQDESNPDFVVEVVSLFFEDSEKLLNDLTRALEQPTVDFK 1278

BLAST of Sgr016159 vs. NCBI nr
Match: RXH72084.1 (hypothetical protein DVH24_025585 [Malus domestica])

HSP 1 Score: 1000.3 bits (2585), Expect = 1.6e-287
Identity = 650/1382 (47.03%), Postives = 750/1382 (54.27%), Query Frame = 0

Query: 162  LPPSRHSSVQINTSETVNSLKEDHDTD--------------------------------- 221
            LPPSR SSVQ    E  +   ED DT+                                 
Sbjct: 20   LPPSRSSSVQSTFCEAGSRFHEDRDTNGTVLIKILVTKKIAFSYAYSEELFNMSNLIFLY 79

Query: 222  ---------ASRPRISNSLLLASQTSTPRLTPPGSPPISSASVSPTRTSKLFPQSVIQCH 281
                     AS P I++S+LLASQTSTPRLTPPGSPPI SAS SPTR+SK  P S  + H
Sbjct: 80   ILRFLVAMAASIPHIAHSVLLASQTSTPRLTPPGSPPIFSASTSPTRSSK--PVSP-KRH 139

Query: 282  SLSQGGCLIGPRCIRLCSLAIAVQLQALMQEGLLLVLFPHLKWFLKKILKRGDEDVNVVL 341
            S+                                                          
Sbjct: 140  SI---------------------------------------------------------- 199

Query: 342  FRYKELLEWFLFDGARRTRVDGKEFFRQVRSRLSYEQFSAFLTNVKELNAHKQTKENDVE 401
                             TRVDGKEFFRQVRSRLSYEQFSAFL NVKELN +KQTKE+   
Sbjct: 200  -----------------TRVDGKEFFRQVRSRLSYEQFSAFLENVKELNRNKQTKESQ-- 259

Query: 402  LEGRCKFKSDFGASNFGGQSKIERMLKWRREFMPIATHLSLSVILLPSFMENDQETSASS 461
                                                                        
Sbjct: 260  ------------------------------------------------------------ 319

Query: 462  SSSKACERTLLRSKMLEISIIEKLDHRDSADMAGCDPIELDKSGSNDSDDIASENQSNDQ 521
                       R +ML                       L    SN S + A +N+    
Sbjct: 320  -----------RQEML-----------------------LKLYFSNGSRETAKDNEE--- 379

Query: 522  INQVVGTPNV--GGRRAR-GFSDSFSTQELRLLDLENEAK-DESSPRGVLED--CSRSLD 581
              Q    P+   G RRAR GFSDSFS+Q+LR L+L+ +   DE S RGV ED  C RS+ 
Sbjct: 380  -AQATTKPSTLGGSRRARAGFSDSFSSQDLRSLNLDEDVSVDEPSSRGVSEDEYCPRSVG 439

Query: 582  SETTTSRASTSDWEGH-------PDSRNPSHWRGFFRLLKKGPQMRFQTFPPL-KTVPKL 641
            SET++ +ASTSD +          + R   H RGFF +LKKGPQ  F TFPP     PKL
Sbjct: 440  SETSSPKASTSDSDARGSITTSDSNPRANHHRRGFFGMLKKGPQNPFPTFPPKGAKKPKL 499

Query: 642  TRRKSKRIREDMLPVLSPVMNSSIETEFCYFRSSWKNFTLSELQAACNNFSQENLIGEGG 701
            TRRK+KRIRE+ +P  +     S + +FC F+SSWKN++  ELQAA NNFS ENLIGEGG
Sbjct: 500  TRRKTKRIREEFIPQPNSPAPRSFDADFCRFKSSWKNYSFLELQAATNNFSHENLIGEGG 559

Query: 702  YSEVYKGQLVDGQLVAIKRLTRGSQEEMTADFLSELGIIVHVDHPNIASVIGYGVEGGMH 761
            Y++VYKG L DGQ+VAIK LTRG+QEEMTADFLSELG+IVHVDHPNIA +IGYGVEGGMH
Sbjct: 560  YADVYKGTLEDGQIVAIKCLTRGTQEEMTADFLSELGVIVHVDHPNIAKLIGYGVEGGMH 619

Query: 762  LILHLSPHGSLASILYGSKERLDWTTRYKIALGIAEGLLYLHEGCQRRIIHRDIKASNIL 821
            L+LHLSPHGSL+SILYG +E LDW  RY +ALG A+GLLYLHEGCQRRIIH+DIKASNIL
Sbjct: 620  LVLHLSPHGSLSSILYGPRENLDWGIRYNVALGTAKGLLYLHEGCQRRIIHKDIKASNIL 679

Query: 822  LRDDFEPQISDFGLAKWLPDQWTHHTVSKFEGTFGYLPPEFFMHGIVDEKTDVYAYGVLL 881
            L +DFEP ISDFGLAKWLPD WTHH VSKFEGTFGYLPPEFFMHGIVDEKTDVYAYGVL+
Sbjct: 680  LAEDFEPLISDFGLAKWLPDSWTHHVVSKFEGTFGYLPPEFFMHGIVDEKTDVYAYGVLI 739

Query: 882  LELITGRRALDSSQQSLVMWAKPLLSKHDIKELVDPSLVDAYDIGQMNRMISVASMCIHQ 941
            LELITGR+ALD S +SLVMW                                        
Sbjct: 740  LELITGRQALDRSHKSLVMW---------------------------------------- 799

Query: 942  SSIQRPEMSQVVQILKGDEVGLE-LVKRQKSKLQRTYSEELLDAEEYNSTKYLNDLDRHL 1001
                      +V+IL+GDE  LE   KRQKSKLQRTYSEELLDA++YNSTK         
Sbjct: 800  ----------IVEILEGDEGSLEYATKRQKSKLQRTYSEELLDADDYNSTK--------- 859

Query: 1002 EACKPKLRFLLANHSFAIREEAPLRNSGFAEFDIFSDKSLSLSLSLSLSLSTLIRARLCP 1061
                                                                        
Sbjct: 860  ------------------------------------------------------------ 919

Query: 1062 DFALCYLSSCFRSHRLFGNPLVRMLVRQAPLAFVYEGFLTDLECDHLISLARSELKRSEV 1121
                                           AFVYEG LTD E DHLIS+A+SELKRS V
Sbjct: 920  -------------------------------AFVYEGLLTDAEWDHLISIAKSELKRSAV 979

Query: 1122 ADNESGKSKLSTVRTSSGMFISKSKDPIVAGIEDKIAAWTFLPKENGEDMQVLRYEHGQK 1181
            ADN SG+SKLS VRTSSGMFI K+KDPIVAGIEDK+A WTFLPKENGED+QVLRYE GQK
Sbjct: 980  ADNLSGQSKLSEVRTSSGMFIPKAKDPIVAGIEDKLATWTFLPKENGEDIQVLRYEPGQK 1039

Query: 1182 YESHYDYFVDKVNIAWGGHRLATVLMYLSDVTKGGETVFPWQRHDNIRQLICLTLLYNFC 1241
            Y  HYDYFVDKVNIA GGHR+ATVLMYL+DV KGGETVFP                    
Sbjct: 1040 YAPHYDYFVDKVNIARGGHRVATVLMYLTDVAKGGETVFP------------------EA 1055

Query: 1242 EQKFPHRRASETDEDLSECARKGIAVKPRK--VMPYFSL-----------------VLNQ 1301
            EQ    R+A+E D  LSECA+KGIAVKPR+   + +FSL                 +  +
Sbjct: 1100 EQIPSRRKAAEIDHSLSECAKKGIAVKPRRGDALLFFSLTPHAVPDPNSLHAGCPVIEGE 1055

Query: 1302 MLSQTPRVCME----------------------VALSLKEKNGQQQSGFTEAAGF--QSS 1361
              S T  + ++                       AL    KN +   G  +  G+  +S 
Sbjct: 1160 KWSATKWIHVDSFDKNLDAGGNCGDLNESCERWAALGECTKNREYMVGTPDLPGYCRRSC 1055

Query: 1362 EPERQKGKRSKSNHLSL----------SASMEVGLMQRQWIEYTKSLFMEGFLDSQFIEL 1421
            +    + K      LS           S  MEVG MQRQW++YTKSLF+EGFLD QF++L
Sbjct: 1220 KICNSRHKLFSPGWLSFAYSVCVERNKSKDMEVGQMQRQWVDYTKSLFLEGFLDGQFLQL 1055

Query: 1422 QKLQDEGNPDFIVEVVSLFFEDSERLLNDLTRALDEPDVDFQKVDGHVHQLKGSSSSIGA 1434
            Q+LQDE NPDF+VEVVSLFFEDSE+LLNDLTRAL++P VDF++VD HVHQ KGSSSSIGA
Sbjct: 1280 QQLQDESNPDFVVEVVSLFFEDSEKLLNDLTRALEQPTVDFKRVDAHVHQFKGSSSSIGA 1055

BLAST of Sgr016159 vs. NCBI nr
Match: XP_008458519.1 (PREDICTED: receptor-like cytosolic serine/threonine-protein kinase RBK2 [Cucumis melo])

HSP 1 Score: 934.9 bits (2415), Expect = 8.4e-268
Identity = 474/539 (87.94%), Postives = 503/539 (93.32%), Query Frame = 0

Query: 409 MENDQETSASSSSSKACERTLLRSKMLEISIIEKLDHRDSADMAGCDPIELDKSGSNDSD 468
           MENDQE+ ASS+SSK  ERTL RSKMLE+SI+EKLD +DSADMA   PIE++ SGSNDS 
Sbjct: 1   MENDQESPASSTSSKTNERTLQRSKMLEVSIVEKLDRQDSADMATYGPIEIETSGSNDSG 60

Query: 469 DIASENQSNDQINQVVGTPNVGGR-RARGFSDSFSTQELRLLDLENEAKDESSPRGVLED 528
           DI S+NQS DQ NQ  GTP++G R R RGFSDSF++ ELRLLDLE+E KD +SPRGVLED
Sbjct: 61  DIDSQNQSKDQTNQAAGTPSIGSRSRPRGFSDSFASHELRLLDLEDETKDVASPRGVLED 120

Query: 529 CSRSLDSETTTSRASTSDWEGHPDSRNPSHWRGFFRLLKKGPQMRFQTFPPLKTVPKLTR 588
           CSRSLDSETTTSRASTSDWEGHPDSRNPSHWRGFFRLLKKGPQMRFQTFPPLK VPKLTR
Sbjct: 121 CSRSLDSETTTSRASTSDWEGHPDSRNPSHWRGFFRLLKKGPQMRFQTFPPLKAVPKLTR 180

Query: 589 RKSKRIREDMLPVLSPVMNSSIETEFCYFRSSWKNFTLSELQAACNNFSQENLIGEGGYS 648
           RKSKRIREDM+PV+SPVM+SSIETEFCYFRSSWKNFTLSEL+AACNNFSQENLIGEGGYS
Sbjct: 181 RKSKRIREDMIPVVSPVMDSSIETEFCYFRSSWKNFTLSELEAACNNFSQENLIGEGGYS 240

Query: 649 EVYKGQLVDGQLVAIKRLTRGSQEEMTADFLSELGIIVHVDHPNIASVIGYGVEGGMHLI 708
           EVYKGQL DGQLVAIKRL RGSQEEMTADFLSELGIIVHVDHPNIASVIGYGVEGGMHLI
Sbjct: 241 EVYKGQLEDGQLVAIKRLNRGSQEEMTADFLSELGIIVHVDHPNIASVIGYGVEGGMHLI 300

Query: 709 LHLSPHGSLASILYGSKERLDWTTRYKIALGIAEGLLYLHEGCQRRIIHRDIKASNILLR 768
           LHLSPHGSLASILYG KERLDW+TRYKIALG AEGLLYLHEGCQRRIIHRDIKA+NILLR
Sbjct: 301 LHLSPHGSLASILYGPKERLDWSTRYKIALGTAEGLLYLHEGCQRRIIHRDIKAANILLR 360

Query: 769 DDFEPQISDFGLAKWLPDQWTHHTVSKFEGTFGYLPPEFFMHGIVDEKTDVYAYGVLLLE 828
           DDFEPQISDFGLAKWLPDQWTHHTVSKFEGTFGYLPPEFFMHGIVDEKTDVYAYGVLLLE
Sbjct: 361 DDFEPQISDFGLAKWLPDQWTHHTVSKFEGTFGYLPPEFFMHGIVDEKTDVYAYGVLLLE 420

Query: 829 LITGRRALDSSQQSLVMWAKPLLSKHDIKELVDPSLVDAYDIGQMNRMISVASMCIHQSS 888
           LITGRRALDS QQSLVMWAKPLLSK ++KELVD SLVDA+D  QM RMIS+ASMCI+QSS
Sbjct: 421 LITGRRALDSLQQSLVMWAKPLLSKQNLKELVDLSLVDAFDSEQMKRMISIASMCINQSS 480

Query: 889 IQRPEMSQVVQILKGDEV-GLELVKRQKSKLQRTYSEELLDAEEYNSTKYLNDLDRHLE 946
           I RPEMS++VQILKGD V  LELVKRQKSKLQRTYSEELLDAEEYNSTKYL+D+DRHLE
Sbjct: 481 ILRPEMSEIVQILKGDGVYNLELVKRQKSKLQRTYSEELLDAEEYNSTKYLSDIDRHLE 539

BLAST of Sgr016159 vs. NCBI nr
Match: XP_022138330.1 (receptor-like cytosolic serine/threonine-protein kinase RBK2 isoform X1 [Momordica charantia] >XP_022138332.1 receptor-like cytosolic serine/threonine-protein kinase RBK2 isoform X1 [Momordica charantia] >XP_022138333.1 receptor-like cytosolic serine/threonine-protein kinase RBK2 isoform X1 [Momordica charantia] >XP_022138334.1 receptor-like cytosolic serine/threonine-protein kinase RBK2 isoform X1 [Momordica charantia])

HSP 1 Score: 934.1 bits (2413), Expect = 1.4e-267
Identity = 479/566 (84.63%), Postives = 505/566 (89.22%), Query Frame = 0

Query: 384 MLKWRREFMPIATHLSLSVILLPSFMENDQETSASSSSSKACERTLLRSKMLEISIIEKL 443
           MLKWRREFMP ATHLSLS+                  S K  ERTL RSKMLE+SII+KL
Sbjct: 1   MLKWRREFMPTATHLSLSL------------------SLKTSERTLQRSKMLEVSIIDKL 60

Query: 444 DHRDSADMAGCDPIELDKSGSNDSDDIASENQSNDQINQVVGTPNVGGRRARGFSDSFST 503
           DHRDS ++AGCDPIEL  S SN+S D A +N SND+ +QV GTPN+G RR RGFSDSF+T
Sbjct: 61  DHRDSENVAGCDPIELHTSRSNESGDTARKNHSNDETDQVAGTPNLGSRRTRGFSDSFAT 120

Query: 504 QELRLLDLENEAKDESSPRGVLEDCSRSLDSETTTSRASTSDWEGHPDSRNPSHWRGFFR 563
            EL LLDLEN  K ESSPRGVLEDCSRSLDSETTTS  STSDW+GHPDSRN SHWRGFFR
Sbjct: 121 HELILLDLENGGKAESSPRGVLEDCSRSLDSETTTSLTSTSDWDGHPDSRNASHWRGFFR 180

Query: 564 LLKKG---PQMRFQ-TFPPLKTVPKLTRRKSKRIREDMLPVLSPVMNSSIETEFCYFRSS 623
           LLKKG   PQMRFQ  FPPLK VPKLTRRKSKRIREDMLPVLSPV++S+IETEFCYFRSS
Sbjct: 181 LLKKGPQIPQMRFQPAFPPLKAVPKLTRRKSKRIREDMLPVLSPVVSSAIETEFCYFRSS 240

Query: 624 WKNFTLSELQAACNNFSQENLIGEGGYSEVYKGQLVDGQLVAIKRLTRGSQEEMTADFLS 683
           WKNFTLSEL+AA NNFS+ENLIGEGGYSEVYKGQL DGQLVAIKRLTRGSQEEMTADFLS
Sbjct: 241 WKNFTLSELKAASNNFSKENLIGEGGYSEVYKGQLEDGQLVAIKRLTRGSQEEMTADFLS 300

Query: 684 ELGIIVHVDHPNIASVIGYGVEGGMHLILHLSPHGSLASILYGSKERLDWTTRYKIALGI 743
           ELGIIVHVDHPNIA+VIGYGVEGGMHLILHLSPHGSLASILY SKERLDWT RY IALGI
Sbjct: 301 ELGIIVHVDHPNIANVIGYGVEGGMHLILHLSPHGSLASILYESKERLDWTMRYNIALGI 360

Query: 744 AEGLLYLHEGCQRRIIHRDIKASNILLRDDFEPQISDFGLAKWLPDQWTHHTVSKFEGTF 803
           AEGLLYLHEGCQRRIIHRDIKASNILLRDDFEPQISDFGLAKWLPDQWTHHTVSKFEGTF
Sbjct: 361 AEGLLYLHEGCQRRIIHRDIKASNILLRDDFEPQISDFGLAKWLPDQWTHHTVSKFEGTF 420

Query: 804 GYLPPEFFMHGIVDEKTDVYAYGVLLLELITGRRALDSSQQSLVMWAKPLLSKHDIKELV 863
           GYLPPEFFMHGIVDEKTDVYAYGVL+LELITGRRALD+SQQSLVMWAKPLLSKHD+KELV
Sbjct: 421 GYLPPEFFMHGIVDEKTDVYAYGVLILELITGRRALDTSQQSLVMWAKPLLSKHDLKELV 480

Query: 864 DPSLVDAYDIGQMNRMISVASMCIHQSSIQRPEMSQVVQILKGDEVGLELVKRQKSKLQR 923
           D SL DA+D  QM RMIS+ASMCIHQ SIQRPEMS+VVQILKGDEVG ELVKRQKSKLQR
Sbjct: 481 DSSLCDAHDTEQMKRMISIASMCIHQYSIQRPEMSEVVQILKGDEVGKELVKRQKSKLQR 540

Query: 924 TYSEELLDAEEYNSTKYLNDLDRHLE 946
           TYSEELLDAEEYNSTKYL+D+DRHLE
Sbjct: 541 TYSEELLDAEEYNSTKYLSDMDRHLE 548

BLAST of Sgr016159 vs. NCBI nr
Match: XP_022138336.1 (receptor-like cytosolic serine/threonine-protein kinase RBK2 isoform X3 [Momordica charantia])

HSP 1 Score: 929.1 bits (2400), Expect = 4.6e-266
Identity = 472/541 (87.25%), Postives = 500/541 (92.42%), Query Frame = 0

Query: 409 MENDQETSASSSSSKACERTLLRSKMLEISIIEKLDHRDSADMAGCDPIELDKSGSNDSD 468
           MEND+E+SASS+SSK  ERTL RSKMLE+SII+KLDHRDS ++AGCDPIEL  S SN+S 
Sbjct: 1   MENDRESSASSTSSKTSERTLQRSKMLEVSIIDKLDHRDSENVAGCDPIELHTSRSNESG 60

Query: 469 DIASENQSNDQINQVVGTPNVGGRRARGFSDSFSTQELRLLDLENEAKDESSPRGVLEDC 528
           D A +N SND+ +QV GTPN+G RR RGFSDSF+T EL LLDLEN  K ESSPRGVLEDC
Sbjct: 61  DTARKNHSNDETDQVAGTPNLGSRRTRGFSDSFATHELILLDLENGGKAESSPRGVLEDC 120

Query: 529 SRSLDSETTTSRASTSDWEGHPDSRNPSHWRGFFRLLKKG---PQMRFQ-TFPPLKTVPK 588
           SRSLDSETTTS  STSDW+GHPDSRN SHWRGFFRLLKKG   PQMRFQ  FPPLK VPK
Sbjct: 121 SRSLDSETTTSLTSTSDWDGHPDSRNASHWRGFFRLLKKGPQIPQMRFQPAFPPLKAVPK 180

Query: 589 LTRRKSKRIREDMLPVLSPVMNSSIETEFCYFRSSWKNFTLSELQAACNNFSQENLIGEG 648
           LTRRKSKRIREDMLPVLSPV++S+IETEFCYFRSSWKNFTLSEL+AA NNFS+ENLIGEG
Sbjct: 181 LTRRKSKRIREDMLPVLSPVVSSAIETEFCYFRSSWKNFTLSELKAASNNFSKENLIGEG 240

Query: 649 GYSEVYKGQLVDGQLVAIKRLTRGSQEEMTADFLSELGIIVHVDHPNIASVIGYGVEGGM 708
           GYSEVYKGQL DGQLVAIKRLTRGSQEEMTADFLSELGIIVHVDHPNIA+VIGYGVEGGM
Sbjct: 241 GYSEVYKGQLEDGQLVAIKRLTRGSQEEMTADFLSELGIIVHVDHPNIANVIGYGVEGGM 300

Query: 709 HLILHLSPHGSLASILYGSKERLDWTTRYKIALGIAEGLLYLHEGCQRRIIHRDIKASNI 768
           HLILHLSPHGSLASILY SKERLDWT RY IALGIAEGLLYLHEGCQRRIIHRDIKASNI
Sbjct: 301 HLILHLSPHGSLASILYESKERLDWTMRYNIALGIAEGLLYLHEGCQRRIIHRDIKASNI 360

Query: 769 LLRDDFEPQISDFGLAKWLPDQWTHHTVSKFEGTFGYLPPEFFMHGIVDEKTDVYAYGVL 828
           LLRDDFEPQISDFGLAKWLPDQWTHHTVSKFEGTFGYLPPEFFMHGIVDEKTDVYAYGVL
Sbjct: 361 LLRDDFEPQISDFGLAKWLPDQWTHHTVSKFEGTFGYLPPEFFMHGIVDEKTDVYAYGVL 420

Query: 829 LLELITGRRALDSSQQSLVMWAKPLLSKHDIKELVDPSLVDAYDIGQMNRMISVASMCIH 888
           +LELITGRRALD+SQQSLVMWAKPLLSKHD+KELVD SL DA+D  QM RMIS+ASMCIH
Sbjct: 421 ILELITGRRALDTSQQSLVMWAKPLLSKHDLKELVDSSLCDAHDTEQMKRMISIASMCIH 480

Query: 889 QSSIQRPEMSQVVQILKGDEVGLELVKRQKSKLQRTYSEELLDAEEYNSTKYLNDLDRHL 946
           Q SIQRPEMS+VVQILKGDEVG ELVKRQKSKLQRTYSEELLDAEEYNSTKYL+D+DRHL
Sbjct: 481 QYSIQRPEMSEVVQILKGDEVGKELVKRQKSKLQRTYSEELLDAEEYNSTKYLSDMDRHL 540

BLAST of Sgr016159 vs. ExPASy Swiss-Prot
Match: Q8RXC8 (Receptor-like cytosolic serine/threonine-protein kinase RBK2 OS=Arabidopsis thaliana OX=3702 GN=RBK2 PE=1 SV=1)

HSP 1 Score: 477.6 bits (1228), Expect = 4.8e-133
Identity = 255/461 (55.31%), Postives = 323/461 (70.07%), Query Frame = 0

Query: 499 DSFSTQELRLLDLENEAKDESSPRGVLEDC--------SRSLDSETTTSRASTSDWEGHP 558
           +S S  +LRLL+++ E +D  SPRG LE C        + S   +   +R +  + +   
Sbjct: 2   NSASAHDLRLLEVDKEKQDPKSPRGALEACLTRCSISSASSSSDDPPPNREAIDNADADT 61

Query: 559 D-----SRNPSHWRGFFRLLKKGPQMRFQTFPPLK--TVPKLTRRKSKRIREDMLPVLSP 618
           D      R  S+W  FF+L K+    R  +FPPL     P + + KS            P
Sbjct: 62  DVQCKNHRASSNWGKFFKLWKRRSMKRLSSFPPLSGAAPPIIKQNKS----------ADP 121

Query: 619 VMNSSIETEFCYFRSSWKNFTLSELQAACNNFSQENLIGEGGYSEVYKGQLVDGQLVAIK 678
            MN  +  +   F+SS +NF++S+++ A +NFS EN+IG GGY++VY+G L +G+L+A+K
Sbjct: 122 NMNGMVLHDIYDFQSSLQNFSISDIEIATDNFSPENIIGRGGYADVYQGILPEGKLIAVK 181

Query: 679 RLTRGSQEEMTADFLSELGIIVHVDHPNIASVIGYGVEGGMHLILHLSPHGSLASILYG- 738
           RLT+G+ +E TA+FLSELGII HVDHPN A  IG  +EGGMHL+  LSP GSL S+L+G 
Sbjct: 182 RLTKGTPDEQTAEFLSELGIIAHVDHPNTAKFIGCCIEGGMHLVFRLSPLGSLGSLLHGP 241

Query: 739 SKERLDWTTRYKIALGIAEGLLYLHEGCQRRIIHRDIKASNILLRDDFEPQISDFGLAKW 798
           SK +L W+ RY +ALG A+GL+YLHEGCQRRIIHRDIKA NILL +DF+PQI DFGLAKW
Sbjct: 242 SKYKLTWSRRYNVALGTADGLVYLHEGCQRRIIHRDIKADNILLTEDFQPQICDFGLAKW 301

Query: 799 LPDQWTHHTVSKFEGTFGYLPPEFFMHGIVDEKTDVYAYGVLLLELITGRRALDSSQQSL 858
           LP Q THH VSKFEGTFGY  PE+FMHGIVDEKTDV+A+GVLLLELITG  ALD SQQSL
Sbjct: 302 LPKQLTHHNVSKFEGTFGYFAPEYFMHGIVDEKTDVFAFGVLLLELITGHPALDESQQSL 361

Query: 859 VMWAKPLLSKHDIKELVDPSLVDAYDIGQMNRMISVASMCIHQSSIQRPEMSQVVQILKG 918
           V+WAKPLL +  IKELVDPSL D Y+  ++ R+ S AS+CI QSS+ RP MSQVV++L G
Sbjct: 362 VLWAKPLLERKAIKELVDPSLGDEYNREELIRLTSTASLCIDQSSLLRPRMSQVVELLLG 421

Query: 919 DEVGLELVKRQKSK-LQRTYSEELLDAEEYNSTKYLNDLDR 943
            E  +   +  K K +QRTYSEELLD+ EYNSTKYL DLDR
Sbjct: 422 HEDVVMTPREAKIKMMQRTYSEELLDSVEYNSTKYLGDLDR 452

BLAST of Sgr016159 vs. ExPASy Swiss-Prot
Match: Q8H1D6 (Receptor-like cytosolic serine/threonine-protein kinase RBK1 OS=Arabidopsis thaliana OX=3702 GN=RBK1 PE=1 SV=1)

HSP 1 Score: 402.5 bits (1033), Expect = 2.0e-110
Identity = 219/461 (47.51%), Postives = 305/461 (66.16%), Query Frame = 0

Query: 498 SDSFSTQELRLLDLENEAKDESSPRGVLEDCSRSLDSETTTSRASTSDWEGHPDSR---- 557
           S+S + QE+ L   +   +D SSPRGVL   S S +S ++ S  S+ D      S     
Sbjct: 9   SESKNHQEVELHRNDLGLEDSSSPRGVLGMVSDSDNSSSSCSSCSSDDKSSSTSSPFSNT 68

Query: 558 ----NPSH----WRGFFRLLKKGPQMRFQTFPPLKTVPKLTRRKSKRIREDMLPVLSPVM 617
               + SH    W      +KK    RF   P L +  +LTR+  +R +    P L+P  
Sbjct: 69  TKTVSSSHHGLQWNKMIESIKKKSMRRFSVIPLLASY-ELTRKNLRRKQ----PKLTPSE 128

Query: 618 NSSIETEFCYFRSSWKNFTLSELQAACNNFSQENLIGEGGYSEVYKGQLVDGQLVAIKRL 677
           ++     F   + SW+NFT  EL  A + F+ EN+IG+GG++EVYKG L++G+ VAIK+L
Sbjct: 129 SAFTCEAFFMAKPSWRNFTYEELAVATDYFNPENMIGKGGHAEVYKGVLINGETVAIKKL 188

Query: 678 TRGS--QEEMTADFLSELGIIVHVDHPNIASVIGYGVEGGMHLILHLSPHGSLASILYGS 737
              +  +EE  +DFLSELGII HV+HPN A + G+  + G+H +L  +P+GSLAS+L+GS
Sbjct: 189 MSHAKEEEERVSDFLSELGIIAHVNHPNAARLRGFSSDRGLHFVLEYAPYGSLASMLFGS 248

Query: 738 KERLDWTTRYKIALGIAEGLLYLHEGCQRRIIHRDIKASNILLRDDFEPQISDFGLAKWL 797
           +E L+W  RYK+ALGIA+GL YLH  C RRIIHRDIKASNILL  D+E QISDFGLAKWL
Sbjct: 249 EECLEWKIRYKVALGIADGLSYLHNACPRRIIHRDIKASNILLNHDYEAQISDFGLAKWL 308

Query: 798 PDQWTHHTVSKFEGTFGYLPPEFFMHGIVDEKTDVYAYGVLLLELITGRRALD-SSQQSL 857
           P+ W HH V   EGTFGYL PE+FMHGIVDEK DV+A+GVLLLE+IT RRA+D +S+QS+
Sbjct: 309 PENWPHHVVFPIEGTFGYLAPEYFMHGIVDEKIDVFAFGVLLLEIITSRRAVDTASRQSI 368

Query: 858 VMWAKPLLSKHDIKELVDPSLVDAYDIGQMNRMISVASMCIHQSSIQRPEMSQVVQILKG 917
           V WAKP L K+ ++++VDP L + ++  +M R++  ASMC+H  +  RP+M+++VQ+L+G
Sbjct: 369 VAWAKPFLEKNSMEDIVDPRLGNMFNPTEMQRVMLTASMCVHHIAAMRPDMTRLVQLLRG 428

Query: 918 DEVGLELVKRQKSKLQRTYSEELLDAEEYNSTKYLNDLDRH 944
           ++   EL   Q+   +RT S    D +++ S+ YLN+L RH
Sbjct: 429 EDGPAEL---QQKAGERTMSVNACDLQDHTSSSYLNELRRH 461

BLAST of Sgr016159 vs. ExPASy Swiss-Prot
Match: Q5XF57 (Probable receptor-like serine/threonine-protein kinase At5g57670 OS=Arabidopsis thaliana OX=3702 GN=At5g57670 PE=2 SV=1)

HSP 1 Score: 322.4 bits (825), Expect = 2.6e-86
Identity = 152/280 (54.29%), Postives = 206/280 (73.57%), Query Frame = 0

Query: 623 FTLSELQAACNNFSQENLIGEGGYSEVYKGQLVDGQLVAIKRLTRGSQE-EMTADFLSEL 682
           FT +E+  A N+F Q N++G GGYSEVY+G L DG+ +A+KRL + S +     +FL+EL
Sbjct: 255 FTYNEISKATNDFHQGNIVGIGGYSEVYRGDLWDGRRIAVKRLAKESGDMNKEKEFLTEL 314

Query: 683 GIIVHVDHPNIASVIGYGVEGGMHLILHLSPHGSLASILYGSKE-RLDWTTRYKIALGIA 742
           GII HV HPN A ++G  VE G++L+   S +G+L S L+ ++   LDW  RYKIA+G+A
Sbjct: 315 GIISHVSHPNTALLLGCCVEKGLYLVFRFSENGTLYSALHENENGSLDWPVRYKIAVGVA 374

Query: 743 EGLLYLHEGCQRRIIHRDIKASNILLRDDFEPQISDFGLAKWLPDQWTHHTVSKFEGTFG 802
            GL YLH+ C  RIIHRDIK+SN+LL  D+EPQI+DFGLAKWLP++WTHH V   EGTFG
Sbjct: 375 RGLHYLHKRCNHRIIHRDIKSSNVLLGPDYEPQITDFGLAKWLPNKWTHHAVIPVEGTFG 434

Query: 803 YLPPEFFMHGIVDEKTDVYAYGVLLLELITGRRALDSSQQSLVMWAKPLLSKHDIKELVD 862
           YL PE  M G +DEKTD+YA+G+LLLE+ITGRR ++ +Q+ +++WAKP +   +  ELVD
Sbjct: 435 YLAPESLMQGTIDEKTDIYAFGILLLEIITGRRPVNPTQKHILLWAKPAMETGNTSELVD 494

Query: 863 PSLVDAYDIGQMNRMISVASMCIHQSSIQRPEMSQVVQIL 901
           P L D YD  QMN+++  AS C+ QS I RP M+QV+++L
Sbjct: 495 PKLQDKYDDQQMNKLVLTASHCVQQSPILRPTMTQVLELL 534

BLAST of Sgr016159 vs. ExPASy Swiss-Prot
Match: Q8LAN3 (Probable prolyl 4-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=P4H4 PE=2 SV=1)

HSP 1 Score: 254.2 bits (648), Expect = 8.7e-66
Identity = 129/204 (63.24%), Postives = 152/204 (74.51%), Query Frame = 0

Query: 1012 SSCFRSHRLFGNPLVRMLVRQAPLAFVYEGFLTDLECDHLISLARSELKRSEVADNESGK 1071
            +S   S  +F NP     V   P AFVYEGFLT+LECDH++SLA++ LKRS VADN+SG+
Sbjct: 22   TSLISSSSVFVNPSKVKQVSSKPRAFVYEGFLTELECDHMVSLAKASLKRSAVADNDSGE 81

Query: 1072 SKLSTVRTSSGMFISKSKDPIVAGIEDKIAAWTFLPKENGEDMQVLRYEHGQKYESHYDY 1131
            SK S VRTSSG FISK KDPIV+GIEDKI+ WTFLPKENGED+QVLRYEHGQKY++H+DY
Sbjct: 82   SKFSEVRTSSGTFISKGKDPIVSGIEDKISTWTFLPKENGEDIQVLRYEHGQKYDAHFDY 141

Query: 1132 FVDKVNIAWGGHRLATVLMYLSDVTKGGETVFPWQRHDNIRQLICLTLLYNFCEQKFPHR 1191
            F DKVNI  GGHR+AT+LMYLS+VTKGGETVFP                    + + P R
Sbjct: 142  FHDKVNIVRGGHRMATILMYLSNVTKGGETVFP--------------------DAEIPSR 201

Query: 1192 RA-SETDEDLSECARKGIAVKPRK 1215
            R  SE  EDLS+CA++GIAVKPRK
Sbjct: 202  RVLSENKEDLSDCAKRGIAVKPRK 205

BLAST of Sgr016159 vs. ExPASy Swiss-Prot
Match: Q8RWW0 (Receptor-like serine/threonine-protein kinase ALE2 OS=Arabidopsis thaliana OX=3702 GN=ALE2 PE=1 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 4.3e-65
Identity = 135/289 (46.71%), Postives = 192/289 (66.44%), Query Frame = 0

Query: 619 SWKNFTLSELQAACNNFSQENLIGEGGYSEVYKGQLVDGQLVAIKRLTRGSQEEMTADFL 678
           S K FTLSEL+ A + FS + ++GEGG+  VY+G + DG  VA+K LTR +Q     +F+
Sbjct: 333 SVKTFTLSELEKATDRFSAKRVLGEGGFGRVYQGSMEDGTEVAVKLLTRDNQNR-DREFI 392

Query: 679 SELGIIVHVDHPNIASVIGYGVEGGMH-LILHLSPHGSLASILYGSKERLDWTTRYKIAL 738
           +E+ ++  + H N+  +IG  +EG    LI  L  +GS+ S L+  +  LDW  R KIAL
Sbjct: 393 AEVEMLSRLHHRNLVKLIGICIEGRTRCLIYELVHNGSVESHLH--EGTLDWDARLKIAL 452

Query: 739 GIAEGLLYLHEGCQRRIIHRDIKASNILLRDDFEPQISDFGLAKWLPDQWTHHTVSKFEG 798
           G A GL YLHE    R+IHRD KASN+LL DDF P++SDFGLA+    + + H  ++  G
Sbjct: 453 GAARGLAYLHEDSNPRVIHRDFKASNVLLEDDFTPKVSDFGLAR-EATEGSQHISTRVMG 512

Query: 799 TFGYLPPEFFMHGIVDEKTDVYAYGVLLLELITGRRALDSSQ----QSLVMWAKPLLSKH 858
           TFGY+ PE+ M G +  K+DVY+YGV+LLEL+TGRR +D SQ    ++LV WA+PLL+  
Sbjct: 513 TFGYVAPEYAMTGHLLVKSDVYSYGVVLLELLTGRRPVDMSQPSGEENLVTWARPLLANR 572

Query: 859 D-IKELVDPSLVDAYDIGQMNRMISVASMCIHQSSIQRPEMSQVVQILK 902
           + +++LVDP+L   Y+   M ++ ++ASMC+HQ    RP M +VVQ LK
Sbjct: 573 EGLEQLVDPALAGTYNFDDMAKVAAIASMCVHQEVSHRPFMGEVVQALK 617

BLAST of Sgr016159 vs. ExPASy TrEMBL
Match: A0A498IAV7 (Uncharacterized protein OS=Malus domestica OX=3750 GN=DVH24_041457 PE=4 SV=1)

HSP 1 Score: 1261.5 bits (3263), Expect = 0.0e+00
Identity = 801/1520 (52.70%), Postives = 934/1520 (61.45%), Query Frame = 0

Query: 1    MREQDSGVANFGLPEEVLEVLPSDPFEQLDVARKITSIALSTRVSALESETSALHAQLSD 60
            M  ++SG +NF LPEEVLEVLP DPFEQLDVARKITS+ALSTRVSALESE+SAL  +L++
Sbjct: 1    MLAKESGSSNFDLPEEVLEVLPPDPFEQLDVARKITSLALSTRVSALESESSALREKLAE 60

Query: 61   KDVLISDLQSQIESLDAALSEKSGKLARADQEKETLLQENASLSNTVKKLTRDVSKLEVF 120
            KD LI+DLQSQ+ESLDA+LSE + KLA A+QEKE L++E A L+NTV+KL+RDVSKLEVF
Sbjct: 61   KDRLIADLQSQVESLDASLSESADKLALANQEKEGLVKEKAVLANTVRKLSRDVSKLEVF 120

Query: 121  RKTLMQSLHE-EENSTGGSEAAAK----LESQESLPSAPHIGDD------LTLPPSRHSS 180
            RKTLM+SL E EEN +G S+  AK    ++++E   S PH GD        TLPPSR SS
Sbjct: 121  RKTLMRSLAEDEENPSGASDVVAKPANTVQAEEHSSSKPHDGDGEGDVTFSTLPPSRSSS 180

Query: 181  VQINTSETVNSLKEDHDTDASRPRISNSLLLASQTSTPRLTPPGSPPISSASVSPTRTSK 240
            VQ +T    ++  ED DTDASRPRI++SLLLASQTSTPR TPPGSPPI SAS SPTRTSK
Sbjct: 181  VQ-STFSGGSTFTEDRDTDASRPRIAHSLLLASQTSTPRFTPPGSPPIYSASTSPTRTSK 240

Query: 241  LFPQSVIQCHSLSQGGCLIGPRCIRLCSLAIAVQLQALMQEGLLLVLFPHLKWFLKKILK 300
                                P   +  S++ A      M +    V   H          
Sbjct: 241  --------------------PGSPKRHSMSFATSRG--MFDNRSSVPSSH---------- 300

Query: 301  RGDEDVNVVLFRYKELLEWFLFDGARRTRVDGKEFFRQVRSRLSYEQFSAFLTNVKELNA 360
             G E                    + RTRVDGKEFFRQVRSRLSYEQF AFL NVKELN 
Sbjct: 301  HGSE--------------------SGRTRVDGKEFFRQVRSRLSYEQFGAFLANVKELNG 360

Query: 361  HKQTKENDVELEGRCKFKSDFGASNFGGQSKIERMLKWRREFMPIATHLSLSVILLPSFM 420
            HKQTKE                     G S++                            
Sbjct: 361  HKQTKEI--------------------GYSQV---------------------------- 420

Query: 421  ENDQETSASSSSSKACERTLLRSKMLEISIIEKLDHRDSADMAGCDPIELDKSGSNDSDD 480
             ND+E   +SS          R  ML                       L    SN S +
Sbjct: 421  HNDKEWLFTSSQ---------RQGML-----------------------LKLHFSNGSRE 480

Query: 481  IASENQSNDQINQVVGTPNVGGRRAR-GFSDSFSTQELRLLDLENEAK-DESSPRGVLED 540
             A ENQ   +      + + G RRAR GFS+SFS+Q+LR L+L+ +    E S RGV ED
Sbjct: 481  TAKENQ---EAQATTPSAHGGSRRARAGFSESFSSQDLRSLNLDEDVSVHELSSRGVSED 540

Query: 541  --CSRSLDSETTTSRASTSDWEGH--------PDSRNPSHWRGFFRLLKKGPQMRFQTFP 600
              C RSL S  ++   STSD +G          D R    WRGFFR+LKKGPQ  F TFP
Sbjct: 541  EYCPRSLGSGASSPMTSTSDSDGRGSRTATSDSDPRANYQWRGFFRILKKGPQYPFPTFP 600

Query: 601  PL-KTVPKLTRRKSKRIREDMLPVLSPVMNSSIETEFCYFRSSWKNFTLSELQAACNNFS 660
            P     PKLTRRKSK+I  D +P ++     S + +FC F+SSWKN++L EL+AA NNFS
Sbjct: 601  PKGAQKPKLTRRKSKKI--DFIPQINSPTLRSFDADFCRFKSSWKNYSLLELEAATNNFS 660

Query: 661  QENLIGEGGYSEVYKGQLVDGQLVAIKRLTRGSQEEMTADFLSELGIIVHVDHPNIASVI 720
             ENLIGEGGY+EVYKG L DGQ+VAIKRLTRGSQEEMTADFLSELG+IVHVDHPNIA +I
Sbjct: 661  HENLIGEGGYAEVYKGTLEDGQIVAIKRLTRGSQEEMTADFLSELGVIVHVDHPNIAKLI 720

Query: 721  GYGVEGGMHLILHLSPHGSLASILYGSKERLDWTTRYKIALGIAEGLLYLHEGCQRRIIH 780
            GYGVEGGMHL+LHLSPHGSL+SILYG +E LDW  RYK+A G A+GLLYLHEGCQRRIIH
Sbjct: 721  GYGVEGGMHLVLHLSPHGSLSSILYGPRENLDWDIRYKVAFGTAKGLLYLHEGCQRRIIH 780

Query: 781  RDIKASNILLRDDFEPQISDFGLAKWLPDQWTHHTVSKFEGTFGYLPPEFFMHGIVDEKT 840
            +DIKASNILL +DFEPQISDFGLAKWLPD WTHH VSKFEGTFGYLPPEFFMHGIVDEKT
Sbjct: 781  KDIKASNILLAEDFEPQISDFGLAKWLPDSWTHHVVSKFEGTFGYLPPEFFMHGIVDEKT 840

Query: 841  DVYAYGVLLLELITGRRALDSSQQSLVMWAKPLLSKHDIKELVDPSLVDAYDIGQMNRMI 900
            DVYAYGVLLLELITGR+ALDSS +SLVMW                               
Sbjct: 841  DVYAYGVLLLELITGRQALDSSHKSLVMW------------------------------- 900

Query: 901  SVASMCIHQSSIQRPEMSQVVQILKGDEVGLE-LVKRQKSKLQRTYSEELLDAEEYNSTK 960
                               +VQIL+GDE  LE   K  KSKLQRTYSEELLDA+EYNSTK
Sbjct: 901  -------------------IVQILEGDETSLEYATKYHKSKLQRTYSEELLDADEYNSTK 960

Query: 961  YLNDLDRHLEACKPKLRFLLANHSFAIREEAPLRNSGFAEFDIFSDKSLSLSLSLSLSLS 1020
            YL+D D+ LE       F+L   +   R                    + L L L L   
Sbjct: 961  YLSDRDKQLE-------FILGTSTLMTR--------------------VCLHLLLLLLFF 1020

Query: 1021 TLIRARLCPDFALCYLSSCFRSHRLFGNPLVRMLVRQAPLAFVYEGFLTDLECDHLISLA 1080
             L    +C   +    SS   S     NP     +   P AFVYEG LTD ECDHLIS+A
Sbjct: 1021 LL---SICSTSS----SSSSASRTFTVNPSKVKQISWNPRAFVYEGLLTDAECDHLISIA 1080

Query: 1081 RSELKRSEVADNESGKSKLSTVRTSSGMFISKSKDPIVAGIEDKIAAWTFLPKENGEDMQ 1140
            +SELKRS VADN SG+SKLS VRTSSGMFI K+KDPIVAGIEDK++ WTFLPKENGED+Q
Sbjct: 1081 KSELKRSAVADNLSGQSKLSEVRTSSGMFIPKAKDPIVAGIEDKLSTWTFLPKENGEDIQ 1140

Query: 1141 VLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSDVTKGGETVFPWQRHDNIRQLI 1200
            VLRYE GQKYE HYDYFVDKVNIA GGHR+ATVLMYL+DV KGGETVFP           
Sbjct: 1141 VLRYEPGQKYEPHYDYFVDKVNIARGGHRIATVLMYLTDVAKGGETVFP----------- 1200

Query: 1201 CLTLLYNFCEQKFP-HRRASETDEDLSECARKGIAVKPRK--VMPYFSL----------- 1260
                     E + P  R+A+E D  LSECA+KGIAVKP++   + +FSL           
Sbjct: 1201 ---------EAEIPLRRKAAEIDHSLSECAKKGIAVKPQRGDALLFFSLTPHAVPDPNSL 1260

Query: 1261 ------VLNQMLSQTPRVCME----------------------VALSLKEKNGQQQSGFT 1320
                  +  +  S T  + ++                       AL    KN +   G  
Sbjct: 1261 HAGCPVIEGEKWSATKWIHVDSFDKNLDASGDCGDLNESCERWAALGECTKNQEYMVGTP 1278

Query: 1321 EAAGFQSSE----------PERQKGKRSKSNHLSLSAS----------MEVGLMQRQWIE 1380
            E  G+               + Q   ++    LS S S          MEVG MQRQW++
Sbjct: 1321 ELPGYCRRSCKSLICAIIPQDLQFKAQAFLCWLSFSYSVCVERNKRKDMEVGQMQRQWVD 1278

Query: 1381 YTKSLFMEGFLDSQFIELQKLQDEGNPDFIVEVVSLFFEDSERLLNDLTRALDEPDVDFQ 1434
            YTKSLF+EGFLD QF++LQ+LQDE NPDF+VEVVSLFFEDSE+LLNDLTRAL++P VDF+
Sbjct: 1381 YTKSLFLEGFLDGQFLQLQQLQDESNPDFVVEVVSLFFEDSEKLLNDLTRALEQPTVDFK 1278

BLAST of Sgr016159 vs. ExPASy TrEMBL
Match: A0A1S3C8M0 (receptor-like cytosolic serine/threonine-protein kinase RBK2 OS=Cucumis melo OX=3656 GN=LOC103497902 PE=4 SV=1)

HSP 1 Score: 934.9 bits (2415), Expect = 4.1e-268
Identity = 474/539 (87.94%), Postives = 503/539 (93.32%), Query Frame = 0

Query: 409 MENDQETSASSSSSKACERTLLRSKMLEISIIEKLDHRDSADMAGCDPIELDKSGSNDSD 468
           MENDQE+ ASS+SSK  ERTL RSKMLE+SI+EKLD +DSADMA   PIE++ SGSNDS 
Sbjct: 1   MENDQESPASSTSSKTNERTLQRSKMLEVSIVEKLDRQDSADMATYGPIEIETSGSNDSG 60

Query: 469 DIASENQSNDQINQVVGTPNVGGR-RARGFSDSFSTQELRLLDLENEAKDESSPRGVLED 528
           DI S+NQS DQ NQ  GTP++G R R RGFSDSF++ ELRLLDLE+E KD +SPRGVLED
Sbjct: 61  DIDSQNQSKDQTNQAAGTPSIGSRSRPRGFSDSFASHELRLLDLEDETKDVASPRGVLED 120

Query: 529 CSRSLDSETTTSRASTSDWEGHPDSRNPSHWRGFFRLLKKGPQMRFQTFPPLKTVPKLTR 588
           CSRSLDSETTTSRASTSDWEGHPDSRNPSHWRGFFRLLKKGPQMRFQTFPPLK VPKLTR
Sbjct: 121 CSRSLDSETTTSRASTSDWEGHPDSRNPSHWRGFFRLLKKGPQMRFQTFPPLKAVPKLTR 180

Query: 589 RKSKRIREDMLPVLSPVMNSSIETEFCYFRSSWKNFTLSELQAACNNFSQENLIGEGGYS 648
           RKSKRIREDM+PV+SPVM+SSIETEFCYFRSSWKNFTLSEL+AACNNFSQENLIGEGGYS
Sbjct: 181 RKSKRIREDMIPVVSPVMDSSIETEFCYFRSSWKNFTLSELEAACNNFSQENLIGEGGYS 240

Query: 649 EVYKGQLVDGQLVAIKRLTRGSQEEMTADFLSELGIIVHVDHPNIASVIGYGVEGGMHLI 708
           EVYKGQL DGQLVAIKRL RGSQEEMTADFLSELGIIVHVDHPNIASVIGYGVEGGMHLI
Sbjct: 241 EVYKGQLEDGQLVAIKRLNRGSQEEMTADFLSELGIIVHVDHPNIASVIGYGVEGGMHLI 300

Query: 709 LHLSPHGSLASILYGSKERLDWTTRYKIALGIAEGLLYLHEGCQRRIIHRDIKASNILLR 768
           LHLSPHGSLASILYG KERLDW+TRYKIALG AEGLLYLHEGCQRRIIHRDIKA+NILLR
Sbjct: 301 LHLSPHGSLASILYGPKERLDWSTRYKIALGTAEGLLYLHEGCQRRIIHRDIKAANILLR 360

Query: 769 DDFEPQISDFGLAKWLPDQWTHHTVSKFEGTFGYLPPEFFMHGIVDEKTDVYAYGVLLLE 828
           DDFEPQISDFGLAKWLPDQWTHHTVSKFEGTFGYLPPEFFMHGIVDEKTDVYAYGVLLLE
Sbjct: 361 DDFEPQISDFGLAKWLPDQWTHHTVSKFEGTFGYLPPEFFMHGIVDEKTDVYAYGVLLLE 420

Query: 829 LITGRRALDSSQQSLVMWAKPLLSKHDIKELVDPSLVDAYDIGQMNRMISVASMCIHQSS 888
           LITGRRALDS QQSLVMWAKPLLSK ++KELVD SLVDA+D  QM RMIS+ASMCI+QSS
Sbjct: 421 LITGRRALDSLQQSLVMWAKPLLSKQNLKELVDLSLVDAFDSEQMKRMISIASMCINQSS 480

Query: 889 IQRPEMSQVVQILKGDEV-GLELVKRQKSKLQRTYSEELLDAEEYNSTKYLNDLDRHLE 946
           I RPEMS++VQILKGD V  LELVKRQKSKLQRTYSEELLDAEEYNSTKYL+D+DRHLE
Sbjct: 481 ILRPEMSEIVQILKGDGVYNLELVKRQKSKLQRTYSEELLDAEEYNSTKYLSDIDRHLE 539

BLAST of Sgr016159 vs. ExPASy TrEMBL
Match: A0A6J1CAT4 (receptor-like cytosolic serine/threonine-protein kinase RBK2 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111009542 PE=4 SV=1)

HSP 1 Score: 934.1 bits (2413), Expect = 7.0e-268
Identity = 479/566 (84.63%), Postives = 505/566 (89.22%), Query Frame = 0

Query: 384 MLKWRREFMPIATHLSLSVILLPSFMENDQETSASSSSSKACERTLLRSKMLEISIIEKL 443
           MLKWRREFMP ATHLSLS+                  S K  ERTL RSKMLE+SII+KL
Sbjct: 1   MLKWRREFMPTATHLSLSL------------------SLKTSERTLQRSKMLEVSIIDKL 60

Query: 444 DHRDSADMAGCDPIELDKSGSNDSDDIASENQSNDQINQVVGTPNVGGRRARGFSDSFST 503
           DHRDS ++AGCDPIEL  S SN+S D A +N SND+ +QV GTPN+G RR RGFSDSF+T
Sbjct: 61  DHRDSENVAGCDPIELHTSRSNESGDTARKNHSNDETDQVAGTPNLGSRRTRGFSDSFAT 120

Query: 504 QELRLLDLENEAKDESSPRGVLEDCSRSLDSETTTSRASTSDWEGHPDSRNPSHWRGFFR 563
            EL LLDLEN  K ESSPRGVLEDCSRSLDSETTTS  STSDW+GHPDSRN SHWRGFFR
Sbjct: 121 HELILLDLENGGKAESSPRGVLEDCSRSLDSETTTSLTSTSDWDGHPDSRNASHWRGFFR 180

Query: 564 LLKKG---PQMRFQ-TFPPLKTVPKLTRRKSKRIREDMLPVLSPVMNSSIETEFCYFRSS 623
           LLKKG   PQMRFQ  FPPLK VPKLTRRKSKRIREDMLPVLSPV++S+IETEFCYFRSS
Sbjct: 181 LLKKGPQIPQMRFQPAFPPLKAVPKLTRRKSKRIREDMLPVLSPVVSSAIETEFCYFRSS 240

Query: 624 WKNFTLSELQAACNNFSQENLIGEGGYSEVYKGQLVDGQLVAIKRLTRGSQEEMTADFLS 683
           WKNFTLSEL+AA NNFS+ENLIGEGGYSEVYKGQL DGQLVAIKRLTRGSQEEMTADFLS
Sbjct: 241 WKNFTLSELKAASNNFSKENLIGEGGYSEVYKGQLEDGQLVAIKRLTRGSQEEMTADFLS 300

Query: 684 ELGIIVHVDHPNIASVIGYGVEGGMHLILHLSPHGSLASILYGSKERLDWTTRYKIALGI 743
           ELGIIVHVDHPNIA+VIGYGVEGGMHLILHLSPHGSLASILY SKERLDWT RY IALGI
Sbjct: 301 ELGIIVHVDHPNIANVIGYGVEGGMHLILHLSPHGSLASILYESKERLDWTMRYNIALGI 360

Query: 744 AEGLLYLHEGCQRRIIHRDIKASNILLRDDFEPQISDFGLAKWLPDQWTHHTVSKFEGTF 803
           AEGLLYLHEGCQRRIIHRDIKASNILLRDDFEPQISDFGLAKWLPDQWTHHTVSKFEGTF
Sbjct: 361 AEGLLYLHEGCQRRIIHRDIKASNILLRDDFEPQISDFGLAKWLPDQWTHHTVSKFEGTF 420

Query: 804 GYLPPEFFMHGIVDEKTDVYAYGVLLLELITGRRALDSSQQSLVMWAKPLLSKHDIKELV 863
           GYLPPEFFMHGIVDEKTDVYAYGVL+LELITGRRALD+SQQSLVMWAKPLLSKHD+KELV
Sbjct: 421 GYLPPEFFMHGIVDEKTDVYAYGVLILELITGRRALDTSQQSLVMWAKPLLSKHDLKELV 480

Query: 864 DPSLVDAYDIGQMNRMISVASMCIHQSSIQRPEMSQVVQILKGDEVGLELVKRQKSKLQR 923
           D SL DA+D  QM RMIS+ASMCIHQ SIQRPEMS+VVQILKGDEVG ELVKRQKSKLQR
Sbjct: 481 DSSLCDAHDTEQMKRMISIASMCIHQYSIQRPEMSEVVQILKGDEVGKELVKRQKSKLQR 540

Query: 924 TYSEELLDAEEYNSTKYLNDLDRHLE 946
           TYSEELLDAEEYNSTKYL+D+DRHLE
Sbjct: 541 TYSEELLDAEEYNSTKYLSDMDRHLE 548

BLAST of Sgr016159 vs. ExPASy TrEMBL
Match: A0A6J1C9F5 (receptor-like cytosolic serine/threonine-protein kinase RBK2 isoform X3 OS=Momordica charantia OX=3673 GN=LOC111009542 PE=4 SV=1)

HSP 1 Score: 929.1 bits (2400), Expect = 2.2e-266
Identity = 472/541 (87.25%), Postives = 500/541 (92.42%), Query Frame = 0

Query: 409 MENDQETSASSSSSKACERTLLRSKMLEISIIEKLDHRDSADMAGCDPIELDKSGSNDSD 468
           MEND+E+SASS+SSK  ERTL RSKMLE+SII+KLDHRDS ++AGCDPIEL  S SN+S 
Sbjct: 1   MENDRESSASSTSSKTSERTLQRSKMLEVSIIDKLDHRDSENVAGCDPIELHTSRSNESG 60

Query: 469 DIASENQSNDQINQVVGTPNVGGRRARGFSDSFSTQELRLLDLENEAKDESSPRGVLEDC 528
           D A +N SND+ +QV GTPN+G RR RGFSDSF+T EL LLDLEN  K ESSPRGVLEDC
Sbjct: 61  DTARKNHSNDETDQVAGTPNLGSRRTRGFSDSFATHELILLDLENGGKAESSPRGVLEDC 120

Query: 529 SRSLDSETTTSRASTSDWEGHPDSRNPSHWRGFFRLLKKG---PQMRFQ-TFPPLKTVPK 588
           SRSLDSETTTS  STSDW+GHPDSRN SHWRGFFRLLKKG   PQMRFQ  FPPLK VPK
Sbjct: 121 SRSLDSETTTSLTSTSDWDGHPDSRNASHWRGFFRLLKKGPQIPQMRFQPAFPPLKAVPK 180

Query: 589 LTRRKSKRIREDMLPVLSPVMNSSIETEFCYFRSSWKNFTLSELQAACNNFSQENLIGEG 648
           LTRRKSKRIREDMLPVLSPV++S+IETEFCYFRSSWKNFTLSEL+AA NNFS+ENLIGEG
Sbjct: 181 LTRRKSKRIREDMLPVLSPVVSSAIETEFCYFRSSWKNFTLSELKAASNNFSKENLIGEG 240

Query: 649 GYSEVYKGQLVDGQLVAIKRLTRGSQEEMTADFLSELGIIVHVDHPNIASVIGYGVEGGM 708
           GYSEVYKGQL DGQLVAIKRLTRGSQEEMTADFLSELGIIVHVDHPNIA+VIGYGVEGGM
Sbjct: 241 GYSEVYKGQLEDGQLVAIKRLTRGSQEEMTADFLSELGIIVHVDHPNIANVIGYGVEGGM 300

Query: 709 HLILHLSPHGSLASILYGSKERLDWTTRYKIALGIAEGLLYLHEGCQRRIIHRDIKASNI 768
           HLILHLSPHGSLASILY SKERLDWT RY IALGIAEGLLYLHEGCQRRIIHRDIKASNI
Sbjct: 301 HLILHLSPHGSLASILYESKERLDWTMRYNIALGIAEGLLYLHEGCQRRIIHRDIKASNI 360

Query: 769 LLRDDFEPQISDFGLAKWLPDQWTHHTVSKFEGTFGYLPPEFFMHGIVDEKTDVYAYGVL 828
           LLRDDFEPQISDFGLAKWLPDQWTHHTVSKFEGTFGYLPPEFFMHGIVDEKTDVYAYGVL
Sbjct: 361 LLRDDFEPQISDFGLAKWLPDQWTHHTVSKFEGTFGYLPPEFFMHGIVDEKTDVYAYGVL 420

Query: 829 LLELITGRRALDSSQQSLVMWAKPLLSKHDIKELVDPSLVDAYDIGQMNRMISVASMCIH 888
           +LELITGRRALD+SQQSLVMWAKPLLSKHD+KELVD SL DA+D  QM RMIS+ASMCIH
Sbjct: 421 ILELITGRRALDTSQQSLVMWAKPLLSKHDLKELVDSSLCDAHDTEQMKRMISIASMCIH 480

Query: 889 QSSIQRPEMSQVVQILKGDEVGLELVKRQKSKLQRTYSEELLDAEEYNSTKYLNDLDRHL 946
           Q SIQRPEMS+VVQILKGDEVG ELVKRQKSKLQRTYSEELLDAEEYNSTKYL+D+DRHL
Sbjct: 481 QYSIQRPEMSEVVQILKGDEVGKELVKRQKSKLQRTYSEELLDAEEYNSTKYLSDMDRHL 540

BLAST of Sgr016159 vs. ExPASy TrEMBL
Match: A0A0A0KEH8 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G067340 PE=4 SV=1)

HSP 1 Score: 928.7 bits (2399), Expect = 2.9e-266
Identity = 469/539 (87.01%), Postives = 502/539 (93.14%), Query Frame = 0

Query: 409 MENDQETSASSSSSKACERTLLRSKMLEISIIEKLDHRDSADMAGCDPIELDKSGSNDSD 468
           MENDQE+ ASSSS+K  ERTL RSKMLE+SIIEKL+ +DS +MA   PIE+  SGSNDS 
Sbjct: 1   MENDQESPASSSSNKTSERTLQRSKMLEVSIIEKLERQDSGNMATYGPIEIQTSGSNDSG 60

Query: 469 DIASENQSNDQINQVVGTPNVGGR-RARGFSDSFSTQELRLLDLENEAKDESSPRGVLED 528
           D+ ++NQS D+ NQ  GTP++G R R RGFSDSF++ ELRLLDLE+E KD +SPRGVLED
Sbjct: 61  DVDTQNQSKDETNQATGTPSIGSRSRPRGFSDSFASHELRLLDLEDETKDVASPRGVLED 120

Query: 529 CSRSLDSETTTSRASTSDWEGHPDSRNPSHWRGFFRLLKKGPQMRFQTFPPLKTVPKLTR 588
           CSRSLDSETTTSRASTSDWEGHPDSRNPSHWRGFFRLLKKGPQMRFQTFPPLK VPKLTR
Sbjct: 121 CSRSLDSETTTSRASTSDWEGHPDSRNPSHWRGFFRLLKKGPQMRFQTFPPLKAVPKLTR 180

Query: 589 RKSKRIREDMLPVLSPVMNSSIETEFCYFRSSWKNFTLSELQAACNNFSQENLIGEGGYS 648
           RKSKRIREDM+PV+SPV++SSIETEFCYFRSSWKNFTLSEL+ ACNNFSQENLIGEGGYS
Sbjct: 181 RKSKRIREDMIPVVSPVIDSSIETEFCYFRSSWKNFTLSELEVACNNFSQENLIGEGGYS 240

Query: 649 EVYKGQLVDGQLVAIKRLTRGSQEEMTADFLSELGIIVHVDHPNIASVIGYGVEGGMHLI 708
           EVYKGQL DGQLVAIKRL RGSQEEMTADFLSELGIIVHVDHPNIASVIGYGVEGGMHLI
Sbjct: 241 EVYKGQLEDGQLVAIKRLNRGSQEEMTADFLSELGIIVHVDHPNIASVIGYGVEGGMHLI 300

Query: 709 LHLSPHGSLASILYGSKERLDWTTRYKIALGIAEGLLYLHEGCQRRIIHRDIKASNILLR 768
           LHLSPHGSLASILYGSKERLDW+TRYKIALGIAEGLLYLHEGCQRRIIHRDIKA+NILLR
Sbjct: 301 LHLSPHGSLASILYGSKERLDWSTRYKIALGIAEGLLYLHEGCQRRIIHRDIKAANILLR 360

Query: 769 DDFEPQISDFGLAKWLPDQWTHHTVSKFEGTFGYLPPEFFMHGIVDEKTDVYAYGVLLLE 828
           DDFEPQISDFGLAKWLPDQWTHHTVSKFEGTFGYLPPEFFMHGIVDEKTDVYAYGVLLLE
Sbjct: 361 DDFEPQISDFGLAKWLPDQWTHHTVSKFEGTFGYLPPEFFMHGIVDEKTDVYAYGVLLLE 420

Query: 829 LITGRRALDSSQQSLVMWAKPLLSKHDIKELVDPSLVDAYDIGQMNRMISVASMCIHQSS 888
           LITGRRALDS QQSLVMWAKPLLSK ++KELVD SLVDA+D  QM RMIS+ASMCI+QSS
Sbjct: 421 LITGRRALDSLQQSLVMWAKPLLSKQNLKELVDLSLVDAFDTEQMKRMISIASMCINQSS 480

Query: 889 IQRPEMSQVVQILKGDEV-GLELVKRQKSKLQRTYSEELLDAEEYNSTKYLNDLDRHLE 946
           I RPEMS++VQILKGD V  LELVKRQKSKLQRTYSEELLDAEEYNSTKYL+D+DRHLE
Sbjct: 481 ILRPEMSEIVQILKGDGVDNLELVKRQKSKLQRTYSEELLDAEEYNSTKYLSDIDRHLE 539

BLAST of Sgr016159 vs. TAIR 10
Match: AT5G18910.1 (Protein kinase superfamily protein )

HSP 1 Score: 597.8 bits (1540), Expect = 2.3e-170
Identity = 315/497 (63.38%), Postives = 390/497 (78.47%), Query Frame = 0

Query: 462 SGSNDSDDIASENQSNDQINQVVGTPNVGGRRARGFSDSFSTQELRLLDLENEAKDESSP 521
           S S+ S D+  +N+          TP+    RA GFSDSFS+ +L     + E K     
Sbjct: 18  SSSSPSCDVDVDNRGKQL------TPSTRRSRA-GFSDSFSSHDLSSFCRDEEVKTFCPI 77

Query: 522 RGVLED-CSRSLDSETTTSRASTSD-------WEGHPDSRNPS--HW-RGFFRLLKKGPQ 581
           +   +D   RS+++ET++S  STSD          H  S  P+  HW RGFFRLLKKG  
Sbjct: 78  KSESDDELLRSIEAETSSSVVSTSDSSETHHHHHHHHSSGGPNNGHWRRGFFRLLKKGSS 137

Query: 582 -MRFQTFPPLKTVPKLTRRKSKRIREDMLPVLSPVMNSSIETEFCYFRSSWKNFTLSELQ 641
            M F TF PLK VPKLTRRKSKRIR++M+PV+ P +++       YF+ SW+NF+L ++Q
Sbjct: 138 AMPFNTFTPLKGVPKLTRRKSKRIRDNMVPVI-PALDTD---HLFYFKPSWRNFSLRDIQ 197

Query: 642 AACNNFSQENLIGEGGYSEVYKGQLVDGQLVAIKRLTRGSQEEMTADFLSELGIIVHVDH 701
            A N++S+ENLIGEGGY+EVYKGQ+ DGQ+VAIK+LTRGS EEMT D+LSELGIIVHVDH
Sbjct: 198 TATNDYSRENLIGEGGYAEVYKGQMADGQIVAIKKLTRGSAEEMTMDYLSELGIIVHVDH 257

Query: 702 PNIASVIGYGVEGGMHLILHLSPHGSLASILYGSKERLDWTTRYKIALGIAEGLLYLHEG 761
           PNIA +IGY VEGGMHL+L LSP+GSLAS+LY +KE+L+W+ RYK+A+G AEGL YLHEG
Sbjct: 258 PNIAKLIGYCVEGGMHLVLELSPNGSLASLLYEAKEKLNWSMRYKVAMGTAEGLYYLHEG 317

Query: 762 CQRRIIHRDIKASNILLRDDFEPQISDFGLAKWLPDQWTHHTVSKFEGTFGYLPPEFFMH 821
           CQRRIIH+DIKASNILL  +FE QISDFGLAKWLPDQWTHHTVSK EGTFGYLPPEFFMH
Sbjct: 318 CQRRIIHKDIKASNILLTQNFEAQISDFGLAKWLPDQWTHHTVSKVEGTFGYLPPEFFMH 377

Query: 822 GIVDEKTDVYAYGVLLLELITGRRALDSSQQSLVMWAKPLLSKHDIKELVDPSLVDAYDI 881
           GIVDEKTDVYAYGVLLLELITGR+ALDSSQ S+VMWAKPL+ ++ IK+LVDP L D YD+
Sbjct: 378 GIVDEKTDVYAYGVLLLELITGRQALDSSQHSIVMWAKPLIKENKIKQLVDPILEDDYDV 437

Query: 882 GQMNRMISVASMCIHQSSIQRPEMSQVVQILKGDEVGLE-LVKRQKSKLQRTYSEELLDA 941
            +++R++ +AS+CIHQ+S+ RP+MSQVV+IL+GD+  L+ L +R+ SKLQRTYSEELLD 
Sbjct: 438 EELDRLVFIASLCIHQTSMNRPQMSQVVEILRGDKCSLDKLRERENSKLQRTYSEELLDN 497

Query: 942 EEYNSTKYLNDLDRHLE 946
           EEYNST+YLND++RH+E
Sbjct: 498 EEYNSTRYLNDINRHME 503

BLAST of Sgr016159 vs. TAIR 10
Match: AT3G05140.1 (ROP binding protein kinases 2 )

HSP 1 Score: 477.6 bits (1228), Expect = 3.4e-134
Identity = 255/461 (55.31%), Postives = 323/461 (70.07%), Query Frame = 0

Query: 499 DSFSTQELRLLDLENEAKDESSPRGVLEDC--------SRSLDSETTTSRASTSDWEGHP 558
           +S S  +LRLL+++ E +D  SPRG LE C        + S   +   +R +  + +   
Sbjct: 2   NSASAHDLRLLEVDKEKQDPKSPRGALEACLTRCSISSASSSSDDPPPNREAIDNADADT 61

Query: 559 D-----SRNPSHWRGFFRLLKKGPQMRFQTFPPLK--TVPKLTRRKSKRIREDMLPVLSP 618
           D      R  S+W  FF+L K+    R  +FPPL     P + + KS            P
Sbjct: 62  DVQCKNHRASSNWGKFFKLWKRRSMKRLSSFPPLSGAAPPIIKQNKS----------ADP 121

Query: 619 VMNSSIETEFCYFRSSWKNFTLSELQAACNNFSQENLIGEGGYSEVYKGQLVDGQLVAIK 678
            MN  +  +   F+SS +NF++S+++ A +NFS EN+IG GGY++VY+G L +G+L+A+K
Sbjct: 122 NMNGMVLHDIYDFQSSLQNFSISDIEIATDNFSPENIIGRGGYADVYQGILPEGKLIAVK 181

Query: 679 RLTRGSQEEMTADFLSELGIIVHVDHPNIASVIGYGVEGGMHLILHLSPHGSLASILYG- 738
           RLT+G+ +E TA+FLSELGII HVDHPN A  IG  +EGGMHL+  LSP GSL S+L+G 
Sbjct: 182 RLTKGTPDEQTAEFLSELGIIAHVDHPNTAKFIGCCIEGGMHLVFRLSPLGSLGSLLHGP 241

Query: 739 SKERLDWTTRYKIALGIAEGLLYLHEGCQRRIIHRDIKASNILLRDDFEPQISDFGLAKW 798
           SK +L W+ RY +ALG A+GL+YLHEGCQRRIIHRDIKA NILL +DF+PQI DFGLAKW
Sbjct: 242 SKYKLTWSRRYNVALGTADGLVYLHEGCQRRIIHRDIKADNILLTEDFQPQICDFGLAKW 301

Query: 799 LPDQWTHHTVSKFEGTFGYLPPEFFMHGIVDEKTDVYAYGVLLLELITGRRALDSSQQSL 858
           LP Q THH VSKFEGTFGY  PE+FMHGIVDEKTDV+A+GVLLLELITG  ALD SQQSL
Sbjct: 302 LPKQLTHHNVSKFEGTFGYFAPEYFMHGIVDEKTDVFAFGVLLLELITGHPALDESQQSL 361

Query: 859 VMWAKPLLSKHDIKELVDPSLVDAYDIGQMNRMISVASMCIHQSSIQRPEMSQVVQILKG 918
           V+WAKPLL +  IKELVDPSL D Y+  ++ R+ S AS+CI QSS+ RP MSQVV++L G
Sbjct: 362 VLWAKPLLERKAIKELVDPSLGDEYNREELIRLTSTASLCIDQSSLLRPRMSQVVELLLG 421

Query: 919 DEVGLELVKRQKSK-LQRTYSEELLDAEEYNSTKYLNDLDR 943
            E  +   +  K K +QRTYSEELLD+ EYNSTKYL DLDR
Sbjct: 422 HEDVVMTPREAKIKMMQRTYSEELLDSVEYNSTKYLGDLDR 452

BLAST of Sgr016159 vs. TAIR 10
Match: AT5G35960.1 (Protein kinase family protein )

HSP 1 Score: 429.9 bits (1104), Expect = 8.2e-120
Identity = 230/410 (56.10%), Postives = 293/410 (71.46%), Query Frame = 0

Query: 506 LRLLDLENEAKDESSPRGVLEDCSRSLDSETTTSRASTSDWEGHPDSRNPSHWRGFFRLL 565
           L L  +  +  D SSP GVLE+  R      T    S+S+   +P S   S +R   +LL
Sbjct: 19  LLLQSIAMKVPDSSSPTGVLEEFFR------TEEFNSSSETVKNPSS---SRFRKMVQLL 78

Query: 566 KKGPQMRFQTFP-PLKT--VPKLTRRKSKRIREDMLPVLSPVMNSSIETEFCYFRSSWKN 625
           +   +   +    P     V K + R+   +RE++         SS ++ F    S  + 
Sbjct: 79  RSKSKKSLENVKIPFHNNGVIKSSFRRCSSMRENL-------RFSSNDSHF-LLHSPRRI 138

Query: 626 FTLSELQAACNNFSQENLIGEGGYSEVYKGQLVDGQLVAIKRLTRGSQEEMTADFLSELG 685
           FT S+L++A NNFS ENLIG+GGY+EVYKG L +GQ+VAIKRL RG+ EE+  DFLSE+G
Sbjct: 139 FTFSDLKSATNNFSLENLIGKGGYAEVYKGMLPNGQMVAIKRLMRGNSEEIIVDFLSEMG 198

Query: 686 IIVHVDHPNIASVIGYGVEGGMHLILHLSPHGSLASILYGSKERLDWTTRYKIALGIAEG 745
           I+ HV+HPNIA ++GYGVEGGMHL+L LSPHGSLAS+LY SKE++ W+ RYKIALG+AEG
Sbjct: 199 IMAHVNHPNIAKLLGYGVEGGMHLVLELSPHGSLASMLYSSKEKMKWSIRYKIALGVAEG 258

Query: 746 LLYLHEGCQRRIIHRDIKASNILLRDDFEPQISDFGLAKWLPDQWTHHTVSKFEGTFGYL 805
           L+YLH GC RRIIHRDIKA+NILL  DF PQI DFGLAKWLP+ WTHH VSKFEGTFGYL
Sbjct: 259 LVYLHRGCHRRIIHRDIKAANILLTHDFSPQICDFGLAKWLPENWTHHIVSKFEGTFGYL 318

Query: 806 PPEFFMHGIVDEKTDVYAYGVLLLELITGRRALDSSQQSLVMWAKPLLSKHDIKELVDPS 865
            PE+  HGIVDEKTDV+A GVLLLEL+TGRRALD S+QSLV+WAKPL+ K+ I+EL+DPS
Sbjct: 319 APEYLTHGIVDEKTDVFALGVLLLELVTGRRALDYSKQSLVLWAKPLMKKNKIRELIDPS 378

Query: 866 LVDAYDIGQMNRMISVASMCIHQSSIQRPEMSQVVQILKGDEVGLELVKR 913
           L   Y+  Q+  ++  A++ I QSSI+RPEMSQVV+ILKG+   L+ + +
Sbjct: 379 LAGEYEWRQIKLVLLAAALSIQQSSIERPEMSQVVEILKGNLKDLKCIMK 411

BLAST of Sgr016159 vs. TAIR 10
Match: AT5G65530.1 (Protein kinase superfamily protein )

HSP 1 Score: 416.4 bits (1069), Expect = 9.4e-116
Identity = 228/438 (52.05%), Postives = 300/438 (68.49%), Query Frame = 0

Query: 517 DESSPRGVLEDCSRSLDSETTTSRASTS-DWEGHPDSRNPSH------WRGFFRLLKKGP 576
           ++ SPR VLE    S DS+ ++S +S S D    P S  P +      W      +KK  
Sbjct: 26  EDPSPRCVLEIPVMSSDSDNSSSCSSCSPDKSSSPLSTTPPNVSSFHQWNKMIESIKKKS 85

Query: 577 QMRFQTFPPLKTVPKLTRRKSKRIREDMLPVLSPVMNSSIE-TEFCYFRSSWKNFTLSEL 636
             RF   P L +  +LTR+K +       P LSP   +  +  +    + SW+NFT  EL
Sbjct: 86  IRRFSVIPLLASY-ELTRKKKQ-------PKLSPCSENDFDCDQILVAKPSWRNFTFDEL 145

Query: 637 QAACNNFSQENLIGEGGYSEVYKGQLVDGQLVAIKRLTRGSQ--EEMTADFLSELGIIVH 696
            AA +NF+ EN+IG+GG++EVYKG L DG+ VAIK+LTR ++  EE  +DFLSELGII H
Sbjct: 146 VAATDNFNPENMIGKGGHAEVYKGVLPDGETVAIKKLTRHAKEVEERVSDFLSELGIIAH 205

Query: 697 VDHPNIASVIGYGVEGGMHLILHLSPHGSLASILYGSKERLDWTTRYKIALGIAEGLLYL 756
           V+HPN A + G+  + G+H +L  S HGSLAS+L+GS+E LDW  RYK+A+GIA+GL YL
Sbjct: 206 VNHPNAARLRGFSCDRGLHFVLEYSSHGSLASLLFGSEECLDWKKRYKVAMGIADGLSYL 265

Query: 757 HEGCQRRIIHRDIKASNILLRDDFEPQISDFGLAKWLPDQWTHHTVSKFEGTFGYLPPEF 816
           H  C RRIIHRDIKASNILL  D+E QISDFGLAKWLP+ W HH V   EGTFGYL PE+
Sbjct: 266 HNDCPRRIIHRDIKASNILLSQDYEAQISDFGLAKWLPEHWPHHIVFPIEGTFGYLAPEY 325

Query: 817 FMHGIVDEKTDVYAYGVLLLELITGRRALDS-SQQSLVMWAKPLLSKHDIKELVDPSLVD 876
           FMHGIVDEKTDV+A+GVLLLE+ITGRRA+D+ S+QS+VMWAKPLL K++++E+VDP L +
Sbjct: 326 FMHGIVDEKTDVFAFGVLLLEIITGRRAVDTDSRQSIVMWAKPLLEKNNMEEIVDPQLGN 385

Query: 877 AYDIGQMNRMISVASMCIHQSSIQRPEMSQVVQILKGDEVGLELVKRQKSKLQRTYSEEL 936
            +D  +M R++  ASMCIH  S  RP+M+++VQ+L+GD    + +  QK    RT S + 
Sbjct: 386 DFDETEMKRVMQTASMCIHHVSTMRPDMNRLVQLLRGD----DQLAEQKPGGARTVSLDD 445

Query: 937 LDAEEYNSTKYLNDLDRH 944
            D  ++ S+ YLNDL RH
Sbjct: 446 CDL-DHTSSSYLNDLTRH 450

BLAST of Sgr016159 vs. TAIR 10
Match: AT5G10520.1 (ROP binding protein kinases 1 )

HSP 1 Score: 402.5 bits (1033), Expect = 1.4e-111
Identity = 219/461 (47.51%), Postives = 305/461 (66.16%), Query Frame = 0

Query: 498 SDSFSTQELRLLDLENEAKDESSPRGVLEDCSRSLDSETTTSRASTSDWEGHPDSR---- 557
           S+S + QE+ L   +   +D SSPRGVL   S S +S ++ S  S+ D      S     
Sbjct: 9   SESKNHQEVELHRNDLGLEDSSSPRGVLGMVSDSDNSSSSCSSCSSDDKSSSTSSPFSNT 68

Query: 558 ----NPSH----WRGFFRLLKKGPQMRFQTFPPLKTVPKLTRRKSKRIREDMLPVLSPVM 617
               + SH    W      +KK    RF   P L +  +LTR+  +R +    P L+P  
Sbjct: 69  TKTVSSSHHGLQWNKMIESIKKKSMRRFSVIPLLASY-ELTRKNLRRKQ----PKLTPSE 128

Query: 618 NSSIETEFCYFRSSWKNFTLSELQAACNNFSQENLIGEGGYSEVYKGQLVDGQLVAIKRL 677
           ++     F   + SW+NFT  EL  A + F+ EN+IG+GG++EVYKG L++G+ VAIK+L
Sbjct: 129 SAFTCEAFFMAKPSWRNFTYEELAVATDYFNPENMIGKGGHAEVYKGVLINGETVAIKKL 188

Query: 678 TRGS--QEEMTADFLSELGIIVHVDHPNIASVIGYGVEGGMHLILHLSPHGSLASILYGS 737
              +  +EE  +DFLSELGII HV+HPN A + G+  + G+H +L  +P+GSLAS+L+GS
Sbjct: 189 MSHAKEEEERVSDFLSELGIIAHVNHPNAARLRGFSSDRGLHFVLEYAPYGSLASMLFGS 248

Query: 738 KERLDWTTRYKIALGIAEGLLYLHEGCQRRIIHRDIKASNILLRDDFEPQISDFGLAKWL 797
           +E L+W  RYK+ALGIA+GL YLH  C RRIIHRDIKASNILL  D+E QISDFGLAKWL
Sbjct: 249 EECLEWKIRYKVALGIADGLSYLHNACPRRIIHRDIKASNILLNHDYEAQISDFGLAKWL 308

Query: 798 PDQWTHHTVSKFEGTFGYLPPEFFMHGIVDEKTDVYAYGVLLLELITGRRALD-SSQQSL 857
           P+ W HH V   EGTFGYL PE+FMHGIVDEK DV+A+GVLLLE+IT RRA+D +S+QS+
Sbjct: 309 PENWPHHVVFPIEGTFGYLAPEYFMHGIVDEKIDVFAFGVLLLEIITSRRAVDTASRQSI 368

Query: 858 VMWAKPLLSKHDIKELVDPSLVDAYDIGQMNRMISVASMCIHQSSIQRPEMSQVVQILKG 917
           V WAKP L K+ ++++VDP L + ++  +M R++  ASMC+H  +  RP+M+++VQ+L+G
Sbjct: 369 VAWAKPFLEKNSMEDIVDPRLGNMFNPTEMQRVMLTASMCVHHIAAMRPDMTRLVQLLRG 428

Query: 918 DEVGLELVKRQKSKLQRTYSEELLDAEEYNSTKYLNDLDRH 944
           ++   EL   Q+   +RT S    D +++ S+ YLN+L RH
Sbjct: 429 EDGPAEL---QQKAGERTMSVNACDLQDHTSSSYLNELRRH 461