Sgr016142 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr016142 vs. NCBI nr
Match: KAG6593916.1 (hypothetical protein SDJN03_13392, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 477.2 bits (1227), Expect = 1.1e-130
Identity = 268/339 (79.06%), Postives = 276/339 (81.42%), Query Frame = 0

Query: 10  NLANLGGLRARLSLKHSISDHNDEEEECRPWRRRLFMAFTWRSALKITLLLLLLAAVVTA 69
           NLANLGGLRARLSLKHS+SDHND+EE CRPWRRRLFMAFTWRSALKIT  LLLLAAVVTA
Sbjct: 65  NLANLGGLRARLSLKHSVSDHNDDEEVCRPWRRRLFMAFTWRSALKITAALLLLAAVVTA 124

Query: 70  CFTLPIEKV---------------------CCYIPLTVLAVPASILTLGGGYLFGLPVGF 129
           CFTLPIEK+                       YIPLTVLAVPASILTLGGGYLFGLPVGF
Sbjct: 125 CFTLPIEKILKDFLLWVDQDLGAWGPLVLSVAYIPLTVLAVPASILTLGGGYLFGLPVGF 184

Query: 130 VADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQFNSVAIAINRSGFKIVLLLRLVPLL 189
           VADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF+SVAIAINRSGFKIVLLLRLVPLL
Sbjct: 185 VADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQFHSVAIAINRSGFKIVLLLRLVPLL 244

Query: 190 PFNMLNYLLSVTPVSLGEYMLASWLGMMPITLALVYVGTTLKDLSDVTHGWGEFSKTRCA 249
           PFNMLNYLLSVTPVSLGEYMLASWLGMMPIT ALVYVGTTLKDLSDVTHGWGEFSKTR A
Sbjct: 245 PFNMLNYLLSVTPVSLGEYMLASWLGMMPITFALVYVGTTLKDLSDVTHGWGEFSKTRWA 304

Query: 250 FIILGLVVSVNRDIELDSIKDKKEGRYQAFSDIDLHAVVLAYCVTRVAKAALEKALAEHE 309
           FI+LGLVVS                            VVLAYCVTRVAKAALEKALAE+ 
Sbjct: 305 FIMLGLVVS----------------------------VVLAYCVTRVAKAALEKALAENG 364

Query: 310 DADYNMGFSSQLPIVAES-PVDLNQPLIIKIDSPQDNCK 327
           DA   +G SSQLPIVAES PVDLNQPLIIKIDSP D+CK
Sbjct: 365 DAVDYLGISSQLPIVAESAPVDLNQPLIIKIDSPPDDCK 375

BLAST of Sgr016142 vs. NCBI nr
Match: KAG6575014.1 (hypothetical protein SDJN03_25653, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 466.1 bits (1198), Expect = 2.5e-127
Identity = 258/336 (76.79%), Postives = 270/336 (80.36%), Query Frame = 0

Query: 10  NLANLGGLRARLSLKHSISDHNDEEEECRPWRRRLFMAFTWRSALKITLLLLLLAAVVTA 69
           NLANLGGLRARLSLKHS+SDHNDEEEECRPWRRRLFMAFTWRSALKIT LL+L+AA V A
Sbjct: 10  NLANLGGLRARLSLKHSVSDHNDEEEECRPWRRRLFMAFTWRSALKITFLLILVAAAVIA 69

Query: 70  CFTLPIEKV---------------------CCYIPLTVLAVPASILTLGGGYLFGLPVGF 129
           CFTLPIEK+                       YIPLTVLAVPASILTLGGGYLFGLPVGF
Sbjct: 70  CFTLPIEKILKDFLLWVDQDLGAWGPLVLSLAYIPLTVLAVPASILTLGGGYLFGLPVGF 129

Query: 130 VADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQFNSVAIAINRSGFKIVLLLRLVPLL 189
           VADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF+ VAIAINRSGFKIVLLLRLVPLL
Sbjct: 130 VADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQFHLVAIAINRSGFKIVLLLRLVPLL 189

Query: 190 PFNMLNYLLSVTPVSLGEYMLASWLGMMPITLALVYVGTTLKDLSDVTHGWGEFSKTRCA 249
           PFNMLNYLLSVTPVSLGEYMLASWLGMMPITLALVYVGTTLKDLSDVTHGWGE SKTR A
Sbjct: 190 PFNMLNYLLSVTPVSLGEYMLASWLGMMPITLALVYVGTTLKDLSDVTHGWGELSKTRLA 249

Query: 250 FIILGLVVSVNRDIELDSIKDKKEGRYQAFSDIDLHAVVLAYCVTRVAKAALEKALAEHE 309
           FIILGLVVS                             +LAYC+TRVAKAALEKALAE+ 
Sbjct: 250 FIILGLVVS----------------------------ALLAYCITRVAKAALEKALAENG 309

Query: 310 DADYNMGFSSQLPIVAESPVDLNQPLIIKIDSPQDN 325
           DA   +G S +LP+ AESPVDLN PLIIKIDSPQD+
Sbjct: 310 DAVDYLGLSPRLPVAAESPVDLNLPLIIKIDSPQDD 317

BLAST of Sgr016142 vs. NCBI nr
Match: KAG7013587.1 (hypothetical protein SDJN02_23754, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 453.0 bits (1164), Expect = 2.2e-123
Identity = 258/370 (69.73%), Postives = 270/370 (72.97%), Query Frame = 0

Query: 10  NLANLGGLRARLSLKHSISDHNDEEEECRPWRRRLFMAFTWRSALKITLLLLLLAAVVTA 69
           NLANLGGLRARLSLKHS+SDHNDEEEECRPWRRRLFMAFTWRSALKIT LL+L+AA V A
Sbjct: 9   NLANLGGLRARLSLKHSVSDHNDEEEECRPWRRRLFMAFTWRSALKITFLLILVAAAVIA 68

Query: 70  CFTLPIEKV--------------------------------------------------- 129
           CFTLPIEK                                                    
Sbjct: 69  CFTLPIEKAGRFMTNYSFLIFNLMSYDLYRIDHIFENLTPLQILKDFLLWVDQDLGAWGP 128

Query: 130 ----CCYIPLTVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFV 189
                 YIPLTVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFV
Sbjct: 129 LVLSLAYIPLTVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFV 188

Query: 190 VSKLKDYPQFNSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLG 249
           VSKLKDYPQF+SVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLG
Sbjct: 189 VSKLKDYPQFHSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLG 248

Query: 250 MMPITLALVYVGTTLKDLSDVTHGWGEFSKTRCAFIILGLVVSVNRDIELDSIKDKKEGR 309
           MMPITLALVYVGTTLKDLSDVTHGWGE SKTR AFIILGLVVS                 
Sbjct: 249 MMPITLALVYVGTTLKDLSDVTHGWGELSKTRLAFIILGLVVS----------------- 308

Query: 310 YQAFSDIDLHAVVLAYCVTRVAKAALEKALAEHEDADYNMGFSSQLPIVAESPVDLNQPL 325
                       +LAYC+TRVAKAALEKALAE+ DA   +G S +LP+ AE+PVDLN PL
Sbjct: 309 -----------ALLAYCITRVAKAALEKALAENGDAVDYLGLSPRLPVAAEAPVDLNLPL 350

BLAST of Sgr016142 vs. NCBI nr
Match: KAF3975695.1 (hypothetical protein CMV_001066 [Castanea mollissima])

HSP 1 Score: 437.6 bits (1124), Expect = 9.6e-119
Identity = 246/337 (73.00%), Postives = 264/337 (78.34%), Query Frame = 0

Query: 9   ENLANLGGLRARLSLKHSISDHNDEEEECRPWRRRLFMAFTWRSALKITLLLLLLAAVVT 68
           +NLANLGGLRARLSLK +ISD++ ++EECRPWRRRLFMAFTW SAL+ITLL LLLAAVVT
Sbjct: 115 KNLANLGGLRARLSLKQAISDYSVDDEECRPWRRRLFMAFTWGSALRITLLFLLLAAVVT 174

Query: 69  ACFTLPIEKV---------------------CCYIPLTVLAVPASILTLGGGYLFGLPVG 128
           ACFT PIEK+                       YIPLTVLAVPAS+LTLGGGYLFGLPVG
Sbjct: 175 ACFTFPIEKILKNFLLWVEQDLGPWGPLVLAVAYIPLTVLAVPASVLTLGGGYLFGLPVG 234

Query: 129 FVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQFNSVAIAINRSGFKIVLLLRLVPL 188
           FVADSIGA+VGAGAAFLLGRTIG+SFV+SKLKDYPQF S+AIAI+ SGFKIVLLLRLVPL
Sbjct: 235 FVADSIGASVGAGAAFLLGRTIGRSFVISKLKDYPQFRSIAIAIHASGFKIVLLLRLVPL 294

Query: 189 LPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLALVYVGTTLKDLSDVTHGWGEFSKTRC 248
           LPFNMLNYLLSVTPV LGEYMLASWLGMMPITLALVYVGTTLKDLSDVTHGW EFSKTR 
Sbjct: 295 LPFNMLNYLLSVTPVPLGEYMLASWLGMMPITLALVYVGTTLKDLSDVTHGWSEFSKTRL 354

Query: 249 AFIILGLVVSVNRDIELDSIKDKKEGRYQAFSDIDLHAVVLAYCVTRVAKAALEKALAEH 308
           AFIILGL VS                            VVL  CVT+VAKAALEKALAE+
Sbjct: 355 AFIILGLAVS----------------------------VVLMVCVTKVAKAALEKALAEN 414

Query: 309 EDADYNMGFSSQLPIVAESPVDLNQPLIIKIDSPQDN 325
           ED D  +  S QLPIVAE PVDLNQPLIIKID  +DN
Sbjct: 415 EDIDGVLA-SPQLPIVAEPPVDLNQPLIIKIDPAEDN 422

BLAST of Sgr016142 vs. NCBI nr
Match: XP_042939575.1 (TVP38/TMEM64 family membrane protein slr0305-like [Carya illinoinensis] >KAG2727408.1 hypothetical protein I3760_01G156200 [Carya illinoinensis] >KAG6668294.1 hypothetical protein CIPAW_01G160000 [Carya illinoinensis] >KAG6732069.1 hypothetical protein I3842_01G158600 [Carya illinoinensis] >KAG7996284.1 hypothetical protein I3843_01G151400 [Carya illinoinensis])

HSP 1 Score: 431.8 bits (1109), Expect = 5.3e-117
Identity = 244/336 (72.62%), Postives = 261/336 (77.68%), Query Frame = 0

Query: 10  NLANLGGLRARLSLKHSISDHNDEEEECRPWRRRLFMAFTWRSALKITLLLLLLAAVVTA 69
           NLAN GGLRARLSLK  ISD+ D+EEECRPWRRR+FMAFTW SAL+ITLLLLLLAAVVTA
Sbjct: 17  NLANFGGLRARLSLKQPISDY-DDEEECRPWRRRIFMAFTWGSALRITLLLLLLAAVVTA 76

Query: 70  CFTLPIEKV---------------------CCYIPLTVLAVPASILTLGGGYLFGLPVGF 129
           CFTLP+EK+                       YIPLT+LAVPAS+LTLGGGYLFGLPVGF
Sbjct: 77  CFTLPVEKILKDFLLWVEQDLGPWGPLVLAVAYIPLTILAVPASVLTLGGGYLFGLPVGF 136

Query: 130 VADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQFNSVAIAINRSGFKIVLLLRLVPLL 189
           VADSIGATVGAGAAF+LGRTIGKSFVVSKLKDYP+F SV IAI+RSGFKIVLLLRLVPLL
Sbjct: 137 VADSIGATVGAGAAFILGRTIGKSFVVSKLKDYPRFQSVGIAIHRSGFKIVLLLRLVPLL 196

Query: 190 PFNMLNYLLSVTPVSLGEYMLASWLGMMPITLALVYVGTTLKDLSDVTHGWGEFSKTRCA 249
           PFNMLNYLLSVTPVSLGEYMLASWLGMMPITL LVYVGTTLKDLSDVTHGW EFSKTR A
Sbjct: 197 PFNMLNYLLSVTPVSLGEYMLASWLGMMPITLTLVYVGTTLKDLSDVTHGWNEFSKTRWA 256

Query: 250 FIILGLVVSVNRDIELDSIKDKKEGRYQAFSDIDLHAVVLAYCVTRVAKAALEKALAEHE 309
           F+ILGL VS                             VL  CVT+VAKAALEKALAE+E
Sbjct: 257 FVILGLSVS----------------------------AVLLICVTKVAKAALEKALAEYE 316

Query: 310 DADYNMGFSSQLPIVAESPVDLNQPLIIKIDSPQDN 325
           D D  +  S QLP+VAE  VDLNQPLIIKID  +DN
Sbjct: 317 DLDGILA-SPQLPVVAEPAVDLNQPLIIKIDPSEDN 322

BLAST of Sgr016142 vs. ExPASy Swiss-Prot
Match: Q55909 (TVP38/TMEM64 family membrane protein slr0305 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=slr0305 PE=3 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 2.1e-23
Identity = 68/183 (37.16%), Postives = 107/183 (58.47%), Query Frame = 0

Query: 81  YIPLTVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKD 140
           Y   TV+ +P SILTLG G +FG+ +G +   IGAT+GA AAFL+GR + + +V  K+  
Sbjct: 27  YTVATVVFLPGSILTLGAGVVFGVILGSIYVFIGATLGATAAFLVGRYLARGWVAKKIAG 86

Query: 141 YPQFNSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITL 200
             +F ++  A+ + G KIV+L RL P+ PFN+LNY   +T VSL +Y++ S LGM+P T+
Sbjct: 87  NQKFKAIDEAVGKEGLKIVILTRLSPVFPFNLLNYAYGITNVSLKDYVIGS-LGMIPGTI 146

Query: 201 ALVYVGTTLKDLSDVTHGWGEFSKT-RCAFIILGLVVSVNRDIELDSIKDKKEGRYQAFS 260
             VY+G+    L+ +     + + T +    I+G + +V   I +  I  K        S
Sbjct: 147 MYVYIGSLAGSLATLGTATNQANPTLQWTIRIVGFIATVAVTIYVTKIARKALNEAILTS 206

Query: 261 DID 263
           ++D
Sbjct: 207 EVD 208

BLAST of Sgr016142 vs. ExPASy Swiss-Prot
Match: P76219 (TVP38/TMEM64 family membrane protein YdjX OS=Escherichia coli (strain K12) OX=83333 GN=ydjX PE=3 SV=2)

HSP 1 Score: 69.3 bits (168), Expect = 9.0e-11
Identity = 39/120 (32.50%), Postives = 67/120 (55.83%), Query Frame = 0

Query: 78  VCCYIPLTVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSK 137
           +  +I  T+L +P SIL + GG +FG  +G +   I AT+ +  +FLL R +G+  ++  
Sbjct: 52  ILLFIIATLLLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARWLGRDLLLKY 111

Query: 138 LKDYPQFNSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMP 197
           +     F ++   I R+G   ++L RL+PL P+N+ NY   +T ++   Y L S L  +P
Sbjct: 112 VGHSNTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWPYTLISALTTLP 171

BLAST of Sgr016142 vs. ExPASy Swiss-Prot
Match: Q6YI46 (Transmembrane protein 64 OS=Homo sapiens OX=9606 GN=TMEM64 PE=2 SV=2)

HSP 1 Score: 67.8 bits (164), Expect = 2.6e-10
Identity = 39/124 (31.45%), Postives = 73/124 (58.87%), Query Frame = 0

Query: 93  ILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQFNSV-AIAI 152
           +L +  GYL+G  +G     +G  +G   A ++ + +  ++V ++++   + ++V  +  
Sbjct: 180 VLNVAAGYLYGFVLGMGLMMVGVLIGTFIAHVVCKRLLTAWVAARIQSSEKLSAVIRVVE 239

Query: 153 NRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLALVYVGTTLKD 212
             SG K+V L RL P +PF + N + S+T +SL  Y++AS +G++P  L   Y+GTTL+ 
Sbjct: 240 GGSGLKVVALARLTP-IPFGLQNAVFSITDLSLPNYLMASSVGLLPTQLLNSYLGTTLRT 299

Query: 213 LSDV 216
           + DV
Sbjct: 300 MEDV 302

BLAST of Sgr016142 vs. ExPASy Swiss-Prot
Match: Q3U145 (Transmembrane protein 64 OS=Mus musculus OX=10090 GN=Tmem64 PE=1 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 7.6e-10
Identity = 39/124 (31.45%), Postives = 72/124 (58.06%), Query Frame = 0

Query: 93  ILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQFNSV-AIAI 152
           +L +  GYL+G  +G     +G  +G   A ++ + +  ++V +++++  + ++V  +  
Sbjct: 181 VLNVAAGYLYGFVLGMGLMVVGVLIGTFIAHVVCKRLLTAWVAARIQNSDKLSAVIRVVE 240

Query: 153 NRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLALVYVGTTLKD 212
             SG K+V L RL P +PF + N + S+T V L  Y++AS  G++P  L   Y+GTTL+ 
Sbjct: 241 GGSGLKVVALARLTP-IPFGLQNAVFSITDVPLPSYLMASSAGLLPTQLLNSYLGTTLRT 300

Query: 213 LSDV 216
           + DV
Sbjct: 301 MEDV 303

BLAST of Sgr016142 vs. ExPASy Swiss-Prot
Match: P9WFS2 (TVP38/TMEM64 family membrane protein MT1538 OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=MT1538 PE=3 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 5.1e-06
Identity = 40/133 (30.08%), Postives = 66/133 (49.62%), Query Frame = 0

Query: 74  PIEKVCCYIPLTVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSF 133
           P+  +  +  +TV   P +  TL  G LFG  VG     +G+T  A  A LL R  G  +
Sbjct: 69  PLAFLLVHTVVTVPPFPRTAFTLAAGLLFGSVVGVFIAVVGSTASAVIAMLLVRATG--W 128

Query: 134 VVSKLKDYPQFNSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWL 193
            ++ L      N +   +   G+  +L LRL+P++PF  +NY    + V +  +  A+  
Sbjct: 129 QLNSLVRRRAINRLDERLRERGWLAILSLRLIPVVPFAAINYAAGASGVRILSFAWATLA 188

Query: 194 GMMPITLALVYVG 207
           G++P T A+V +G
Sbjct: 189 GLLPGTAAVVILG 199

BLAST of Sgr016142 vs. ExPASy TrEMBL
Match: A0A498IBM5 (Uncharacterized protein OS=Malus domestica OX=3750 GN=DVH24_041506 PE=4 SV=1)

HSP 1 Score: 421.0 bits (1081), Expect = 4.5e-114
Identity = 235/336 (69.94%), Postives = 260/336 (77.38%), Query Frame = 0

Query: 10  NLANLGGLRARLSLKHSISDHNDEEEECRPWRRRLFMAFTWRSALKITLLLLLLAAVVTA 69
           NLANLGGLR RLSLK  I  +ND++EECRPWRRR  MAFTW SAL+I LLLLLLAAV TA
Sbjct: 62  NLANLGGLRPRLSLKQPI--YNDDDEECRPWRRRFSMAFTWGSALRIALLLLLLAAVATA 121

Query: 70  CFTLPIEKV---------------------CCYIPLTVLAVPASILTLGGGYLFGLPVGF 129
           CFTLP+EK+                       YIPLTVLAVPAS+LTLGGGYLFGLPVGF
Sbjct: 122 CFTLPVEKILKDFLLWVEQDLGPWGPLVLAVAYIPLTVLAVPASVLTLGGGYLFGLPVGF 181

Query: 130 VADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQFNSVAIAINRSGFKIVLLLRLVPLL 189
           VADSIGAT+GAGAAFLLGRTIG+SFVVS+LKDYPQF +VAIAI RSGFKIVLLLRLVPLL
Sbjct: 182 VADSIGATLGAGAAFLLGRTIGRSFVVSRLKDYPQFRAVAIAIRRSGFKIVLLLRLVPLL 241

Query: 190 PFNMLNYLLSVTPVSLGEYMLASWLGMMPITLALVYVGTTLKDLSDVTHGWGEFSKTRCA 249
           PFNMLNYLLSVTPV +G+YMLASW+GMMPIT ALVYVGTTLKDLSDVTHGWGEFSKTR A
Sbjct: 242 PFNMLNYLLSVTPVPIGQYMLASWIGMMPITFALVYVGTTLKDLSDVTHGWGEFSKTRWA 301

Query: 250 FIILGLVVSVNRDIELDSIKDKKEGRYQAFSDIDLHAVVLAYCVTRVAKAALEKALAEHE 309
           FIILGLVVS                            V+L  CVTRVAKAALEKALAE+E
Sbjct: 302 FIILGLVVS----------------------------VILMICVTRVAKAALEKALAENE 361

Query: 310 DADYNMGFSSQLPIVAESPVDLNQPLIIKIDSPQDN 325
           D D N+    +LP+V+E+PVDL+QPL+IKID+  D+
Sbjct: 362 DID-NIDIIPELPVVSETPVDLDQPLLIKIDASDDH 366

BLAST of Sgr016142 vs. ExPASy TrEMBL
Match: A0A5A7SW85 (TVP38/TMEM64 family membrane protein slr0305-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold111G00770 PE=4 SV=1)

HSP 1 Score: 416.4 bits (1069), Expect = 1.1e-112
Identity = 244/339 (71.98%), Postives = 258/339 (76.11%), Query Frame = 0

Query: 6   IIGENLANLGGLRARLSLKHSISDHNDEEEECRPWRRRLFMAFTWRSALKITLLLLLLAA 65
           ++  NLANLGGLRARLSLKHSISDHND+EEECRPWRRRL MAFTWRSALKITLLLLLLAA
Sbjct: 2   VLPGNLANLGGLRARLSLKHSISDHNDDEEECRPWRRRLSMAFTWRSALKITLLLLLLAA 61

Query: 66  VVTACFTLPIEKV---------------------CCYIPLTVLAVPASILTLGGGYLFGL 125
            VTACFTLPIEK+                       YIPLTVLAVPASILTLGGGYLFGL
Sbjct: 62  AVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSIAYIPLTVLAVPASILTLGGGYLFGL 121

Query: 126 PVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQFNSVAIAINRSGFKIVLLLRL 185
           PVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF+SVAIAINRSGFKIVLLLRL
Sbjct: 122 PVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQFHSVAIAINRSGFKIVLLLRL 181

Query: 186 VPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLALVYVGTTLKDLSDVTHGWGEFSK 245
           VPLLPFNMLNYLLSVTPVSLGEYMLASWLGMM    +L ++   L               
Sbjct: 182 VPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMDSGDSLRWLKRNLLGRKKA--------- 241

Query: 246 TRCAFIILGLVVSVNRDIELDSIKDKKEGRY-QAFSDIDLHAVVLAYCVTRVAKAALEKA 305
                   G  +    +I    I  +KEG   + FSDIDLHAVVLAYCVTRVAKAALEKA
Sbjct: 242 --------GRHLPSIHNIGPCGIGFRKEGTVPEGFSDIDLHAVVLAYCVTRVAKAALEKA 301

Query: 306 LAEHEDADYNMGFSSQLPIVAESPVDLNQPLIIKIDSPQ 323
           LAE+ D    +G SSQLPIV ESPVDLNQPLIIKIDSPQ
Sbjct: 302 LAENGDGVDYLGLSSQLPIVDESPVDLNQPLIIKIDSPQ 323

BLAST of Sgr016142 vs. ExPASy TrEMBL
Match: A0A6J1CB63 (uncharacterized protein LOC111009895 OS=Momordica charantia OX=3673 GN=LOC111009895 PE=4 SV=1)

HSP 1 Score: 409.8 bits (1052), Expect = 1.0e-110
Identity = 233/298 (78.19%), Postives = 240/298 (80.54%), Query Frame = 0

Query: 46  MAFTWRSALKITLLLLLLAAVVTACFTLPIEKV---------------------CCYIPL 105
           MAFTWRSALKIT LLLLLAA+VTACFTLPI+K+                       YIPL
Sbjct: 1   MAFTWRSALKITFLLLLLAAIVTACFTLPIDKILKDFLLWVDQDLGAWGPLVLSVAYIPL 60

Query: 106 TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 165
           TVLAVPASILTLGGGYLFGLPVGF+ADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF
Sbjct: 61  TVLAVPASILTLGGGYLFGLPVGFIADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 120

Query: 166 NSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLALVY 225
           +SVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPIT ALVY
Sbjct: 121 HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITFALVY 180

Query: 226 VGTTLKDLSDVTHGWGEFSKTRCAFIILGLVVSVNRDIELDSIKDKKEGRYQAFSDIDLH 285
           VGTTLKDLSDVTHGWGEFSKTRCA IILGLVVS                           
Sbjct: 181 VGTTLKDLSDVTHGWGEFSKTRCALIILGLVVS--------------------------- 240

Query: 286 AVVLAYCVTRVAKAALEKALAEHEDADYNMGFSSQLPIVAESPVDLNQPLIIKIDSPQ 323
            VVLAYCVTRVAKAALEKALAE  DADY MGFSSQLP+VA+S VDLNQPLIIKIDSPQ
Sbjct: 241 -VVLAYCVTRVAKAALEKALAESGDADY-MGFSSQLPLVADSSVDLNQPLIIKIDSPQ 269

BLAST of Sgr016142 vs. ExPASy TrEMBL
Match: A0A371E6I6 (Uncharacterized protein (Fragment) OS=Mucuna pruriens OX=157652 GN=CR513_60113 PE=4 SV=1)

HSP 1 Score: 408.7 bits (1049), Expect = 2.3e-110
Identity = 237/375 (63.20%), Postives = 260/375 (69.33%), Query Frame = 0

Query: 10  NLANLGGLRARLSLKHSISDHNDEEEECRPWRRRLFMAFTWRSALKITLLLLLLAAVVTA 69
           NLANL GLRAR S K +  D ND EEECRPWRRRL M+F+W SAL+IT+LLLLLAAV+TA
Sbjct: 14  NLANLVGLRARSSSKQASPDPNDAEEECRPWRRRLTMSFSWASALRITILLLLLAAVITA 73

Query: 70  CFTLPIEKV--------------------------------------------------- 129
           CFTLP+EK+                                                   
Sbjct: 74  CFTLPVEKLMKDFLLWVDHDLGPWGPFVLYVLSPLFGFLNLLLILSFLILGLLVGLISSC 133

Query: 130 ---------CCYIPLTVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTI 189
                      YIPLTVLAVPAS+LTLGGGYLFGLPVGF+ADSIGATVGAGAAF+LGRTI
Sbjct: 134 FLCSLIFRAVAYIPLTVLAVPASVLTLGGGYLFGLPVGFIADSIGATVGAGAAFILGRTI 193

Query: 190 GKSFVVSKLKDYPQFNSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYML 249
           G+SFVVS+LKDYPQF SVAIAI RSGFKIV LLRLVPLLPFNMLNYLLSVTPVS+GEYML
Sbjct: 194 GRSFVVSRLKDYPQFRSVAIAIRRSGFKIVFLLRLVPLLPFNMLNYLLSVTPVSVGEYML 253

Query: 250 ASWLGMMPITLALVYVGTTLKDLSDVTHGWGEFSKTRCAFIILGLVVSVNRDIELDSIKD 309
           ASWLGMMPITLALVYVGTTLKDLSDVTHGWGEFSKTR AFIILGLV+S            
Sbjct: 254 ASWLGMMPITLALVYVGTTLKDLSDVTHGWGEFSKTRWAFIILGLVIS------------ 313

Query: 310 KKEGRYQAFSDIDLHAVVLAYCVTRVAKAALEKALAEHEDADYNMGFSSQLPIVAESPVD 325
                           VVL  CVT+VAK+AL+KALAE+ED D  +  S +LPIVAES  D
Sbjct: 314 ----------------VVLMICVTKVAKSALDKALAENEDID-GITSSPELPIVAESSAD 359

BLAST of Sgr016142 vs. ExPASy TrEMBL
Match: A0A0A0KC10 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G237630 PE=4 SV=1)

HSP 1 Score: 407.1 bits (1045), Expect = 6.7e-110
Identity = 234/300 (78.00%), Postives = 239/300 (79.67%), Query Frame = 0

Query: 46  MAFTWRSALKITLLLLLLAAVVTACFTLPIEKV---------------------CCYIPL 105
           MAFTWRSALKITLLLLLLAA VTACFTLPIEK+                       YIPL
Sbjct: 1   MAFTWRSALKITLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSIAYIPL 60

Query: 106 TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 165
           TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF
Sbjct: 61  TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 120

Query: 166 NSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLALVY 225
           +SVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITL LVY
Sbjct: 121 HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLTLVY 180

Query: 226 VGTTLKDLSDVTHGWGEFSKTRCAFIILGLVVSVNRDIELDSIKDKKEGRYQAFSDIDLH 285
           VGTTLKDLSDVTHGWGEFSKTR AFIILGLVVS                           
Sbjct: 181 VGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVS--------------------------- 240

Query: 286 AVVLAYCVTRVAKAALEKALAEHEDADYNMGFSSQLPIVAESPVDLNQPLIIKIDSPQDN 325
            VVLAYCVTRVAKAALEKALAE+ D    +G SSQLPIV ESPVDLNQPLIIKIDSPQD+
Sbjct: 241 -VVLAYCVTRVAKAALEKALAENGDGVDYLGLSSQLPIVDESPVDLNQPLIIKIDSPQDD 272

BLAST of Sgr016142 vs. TAIR 10
Match: AT5G19070.1 (SNARE associated Golgi protein family )

HSP 1 Score: 313.9 bits (803), Expect = 1.5e-85
Identity = 180/302 (59.60%), Postives = 214/302 (70.86%), Query Frame = 0

Query: 46  MAFTWRSALKITLLLLLLAAVVTACFTLPIEKV---------------------CCYIPL 105
           MAFTW SAL+I++LL+L+AA+V AC+ LP+EK+                       YIPL
Sbjct: 1   MAFTWGSALRISVLLILVAAIVLACYFLPVEKLLKDFLLWVEQDLGPWGPFALAVAYIPL 60

Query: 106 TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 165
           TVLAVPAS+LTLGGGYLFGLP+GFVADS+GAT+G+GAAFLLGRTIGK FVV+KLKDYPQF
Sbjct: 61  TVLAVPASVLTLGGGYLFGLPIGFVADSVGATLGSGAAFLLGRTIGKPFVVAKLKDYPQF 120

Query: 166 NSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLALVY 225
            SVA+AI +SGFKI LLLRL PLLPF+MLNYLLSVTP+ LG Y+L+SWLGMMPITLALVY
Sbjct: 121 QSVALAIEKSGFKICLLLRLAPLLPFSMLNYLLSVTPIRLGPYLLSSWLGMMPITLALVY 180

Query: 226 VGTTLKDLSDVTHGWGEFSKTRCAFIILGLVVSVNRDIELDSIKDKKEGRYQAFSDIDLH 285
           VGTTLKDLSDVTH W EFS  R AF+I  LV+S                           
Sbjct: 181 VGTTLKDLSDVTHKWSEFSPGRWAFLISSLVIS--------------------------- 240

Query: 286 AVVLAYCVTRVAKAALEKALAEH-EDADYNMGFSSQLPIVAESPVDLNQPLIIKIDS--P 324
            V+L  CVT+VAK AL KALAEH  D +  +    +L +  ++  DLN+PL+IKID+  P
Sbjct: 241 -VILMVCVTKVAKDALRKALAEHGGDMNEAVAALPELTVTDDASTDLNEPLLIKIDAQQP 274

BLAST of Sgr016142 vs. TAIR 10
Match: AT1G03260.1 (SNARE associated Golgi protein family )

HSP 1 Score: 278.5 bits (711), Expect = 6.9e-75
Identity = 161/296 (54.39%), Postives = 202/296 (68.24%), Query Frame = 0

Query: 46  MAFTWRSALKITLLLLLLAAVVTACFTLPIEK---------------------VCCYIPL 105
           M+FT  S  +I + LLLL A+V+A   LP+E+                        YIPL
Sbjct: 4   MSFT-PSTFRIAISLLLLVAIVSAVIFLPVEQKLKDFLLWIKEDLGPFGPLALALAYIPL 63

Query: 106 TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 165
           T++AVPAS+LTLGGGYLFGLPVGFVADS+GAT+GA AAFLLGRTIGKS+V SK+K YP+F
Sbjct: 64  TIVAVPASVLTLGGGYLFGLPVGFVADSLGATLGATAAFLLGRTIGKSYVTSKIKHYPKF 123

Query: 166 NSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLALVY 225
            +V++AI +SGFKIVLLLR+VP+LPFNMLNYLLSVTPV LGEYMLA+WLGMMPIT ALVY
Sbjct: 124 QAVSVAIQKSGFKIVLLLRVVPILPFNMLNYLLSVTPVRLGEYMLATWLGMMPITFALVY 183

Query: 226 VGTTLKDLSDVTHGWGEFSKTRCAFIILGLVVSVNRDIELDSIKDKKEGRYQAFSDIDLH 285
           VGTTLKDLSD+THGW E S  R   +++G+ +                            
Sbjct: 184 VGTTLKDLSDITHGWHEVSVFRWVIMMVGVAL---------------------------- 243

Query: 286 AVVLAYCVTRVAKAALEKALAEH-EDADYNMGFSSQLPIVAESPVDLNQPLIIKID 320
           AV+L  C+TRVAK++L+KALAE+  + D      + +  +AE P DL +PL+I+ID
Sbjct: 244 AVILIICITRVAKSSLDKALAENGTELDGKKNDDASVLPIAEPPPDLQEPLVIRID 270

BLAST of Sgr016142 vs. TAIR 10
Match: AT1G22850.1 (SNARE associated Golgi protein family )

HSP 1 Score: 96.7 bits (239), Expect = 3.7e-20
Identity = 62/178 (34.83%), Postives = 97/178 (54.49%), Query Frame = 0

Query: 78  VCCYIPLTVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSK 137
           +  Y  L +LA+PA  LT+  G LFG  +G +  SI  T+ A  AFL+ R   +  ++  
Sbjct: 157 IAVYAGLEILAIPALPLTMSAGLLFGPLIGTIIVSISGTMAASVAFLIARYFARERILKL 216

Query: 138 LKDYPQFNSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMP 197
           ++D  +F ++  AI  +GF++V LLRL PLLPF++ NYL  +T V    Y+L SWLGM+P
Sbjct: 217 VEDNKKFLAIDKAIGENGFRVVTLLRLSPLLPFSLGNYLYGLTSVKFVPYVLGSWLGMLP 276

Query: 198 ITLALVYVG----TTLKDLSDV--THGWGEFSKTRCAFIILGLVVSVNRDIELDSIKD 250
            + A V  G      +++ S+V    G G+        ++  L  +    +  D+IKD
Sbjct: 277 GSWAYVSAGAFGRAIIQEESNVGLPGGNGQLLTLGVGLLVTALAGTYVTSLAKDAIKD 334

BLAST of Sgr016142 vs. TAIR 10
Match: AT1G12450.1 (SNARE associated Golgi protein family )

HSP 1 Score: 45.4 bits (106), Expect = 9.9e-05
Identity = 53/220 (24.09%), Postives = 89/220 (40.45%), Query Frame = 0

Query: 45  FMAFTWRSALKITLLLLLLAAVVTACFTLPIEKVCCYIPLTV---LAVPASILTLGGGYL 104
           F+   W     I   L+     V   F++P+  +  +  + +   + +P+S      G  
Sbjct: 74  FVIIKWVGPFLIEKELIPFINWVRNTFSIPVLGLLLFASVALFPSILLPSSPSMWMAGLT 133

Query: 105 FGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQFNSVAIAINRS----GFK 164
           FG   GF+     A++G    FL+G       +   LK YP+  ++  A         F+
Sbjct: 134 FGYGKGFLLILSAASIGVTLPFLIGHLFLHK-MQEWLKKYPKKAAILRAAGEGTWFHQFQ 193

Query: 165 IVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLALVYVGTTLKDLSDVTH 224
            V L+R+ P  P+ + NY    T V  G Y+L S +GM+P     +Y G  L+ L+  + 
Sbjct: 194 AVTLIRVSP-FPYIIYNYCALATGVHYGPYILGSLVGMVPEIFVSIYTGIMLRTLAVASD 253

Query: 225 GWGEFSKTRCAFIILGLVVSVNRDIELDSIKDKKEGRYQA 258
                S       +LG  V+ +  I       KK    Q+
Sbjct: 254 TRHTLSVVEIVVNVLGFCVTASATIVCTIYAKKKLSAMQS 291