Sgr016133 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr016133
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Descriptionmitogen-activated protein kinase kinase kinase NPK1
Locationtig00007724: 91225 .. 102026 (-)
RNA-Seq ExpressionSgr016133
SyntenySgr016133
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAAGATTTCTTTGGATCCGTTCGCCGATCCATAGTATTTCTAGCTCCCGACGGAGACGACGGAGGGAGGTTTGGTGGCCTGGTCGAGAAGATCGGCTCGAGCATCCGTAAATCCCGAAATGGGCTGTTTTCTAAGCATTCAGTACGGGCTCTGCCTCCAGTGGCGAAGGAAGATTCACCTCCGATTTGGTGGCGGAAGGGGGAGTTGATCGGATGCGGTGCTTTTGGTCGAGTTTATATGGGGATGAACCTTGATTCTGGAGAGCTTCTTGCAGTCAAACAAGTCAGTAATTTTAGATTTAGTTGTGCAGTTCATTTAGATATAGTGGGTTTTGAACTTTTGATTCTTAAATTCCTCTGCCTCGGTTGCAGGTCTCAATTGCTGCCAATAGTGCTTCAAGGGAGAAAGCACAAGTGAGGACGTTTACTAATTGAACGTTTGCTAGATGTGGAAGACTTGCAGTAGAATTAGACAGCACTTTTTCAGTAATTTTCTACGTTCTAATTGAATTCATCAAATGCACTCTCACGCTTTCTATTTAACATAACTACGTAGGCTCACATACGGGAGCTGGAGGAGGAAGTGAAGCTTTTGAAGAATCTTTCTCACCCCAATATTGTGGTAAGTAAAATCTTATGGTCTTTTCTTTCTTTCCTAAAGTTGTTGGTAGCTCCTTGCTTACTTTCTTATCGTGATTTTTAGAGATATCTGGGAACTGCCAGAGAGGAAGAGTCATTAAATATTCTATTGGAGTTTGTTCCTGGAGGATCAATATCATCTTTGTTAGGGAAATTTGGATCTTTCCCCGAGTCTGTAAGTGATTGTATTTGTATCTTTAATCTTTAAACTTCTCAATATTGGAATAGAATAAATTTAGCGTTGACATTAATGTTGAAATTTTGTAAATGGTTATTGATTTTGTCCAAAAATAGGTTATAAGAACCTACACAAAGCAGCTATTATTGGGACTTGAGTACCTTCATAAGAACGGCATTATGCATAGGGATATAAAGGTGATAGTGAAACCCCTTTTTGAGAAGGAAGAGTTTTAATTTTTAACCTTTGCTTGTCAATTTACCCGGATAATGTATTACTTGACGTAGGGTGCAAACATTCTTGTAGACAATAAGGGGTGTATTAAACTCGCAGATTTTGGTGCATCCAAGAAAGTGGTTGAGCTGGTATTGCCCATTGTTCATCTAATTTAGTTATCTGAATTGATTATTATCATTCTCAATATAAAAATTAGCATCTACGCAGGCAACTATAAATGGAGCCAAATCAATGAAGGGTACTCCATATTGGATGGCTCCTGAAGTTATTCTCCAGACTGGACATAGCTTGTAAGAATTACCATCTTATTGTGAATAGATTTATTTGCTTTTTCACTATTTCACCCCATGCCATTGATATGGATCAATATGTTGTAGTGCATGGGAGGAATACAAATGTGCTGAAACTTATTCTGAAATAATGGAAGAGCAATGAAAGTAACAGGAGAAAAACATGAAATTTTCTTCTTAAGAAACTAATATTTAGATCTCATGTTCTTAATATTTTATGTCTTAGCTCTGCTGATATATGGAGTGTGGGGTGTACTGTTATTGAAATGGCAACAGGGAAGCCTCCATGGAGCCAACAGTATCAAGAGGTAAGCAAGTGGATTTACTTCAATATGCATGTATGTGCTTACGTTTTCTTCTAATACCTTATGACTTGAGTCAATGTTATTTTCAGGTTGCTGCTCTTTTCCATATAGGGAATACTAAGTCCCATCCGCCCATTCCTGAGCATCTATCTGCTGAGGCAAAAGATTTTCTGTTAAAATGTTTACAAGAGTACTTTCGAAACTCTGACTTTAGTTCTTCACTTGAATTTTATTTTTTTATTTTTTGTCTTTTTAACTTTGAATTTCTTTCTGAAAGATTGATTGAAATATATATACTATTCTCTGTTTAATTTAACTTCACCTTATTATAAATATTCAAATGTGCTCACAGGGAGCCAAACTCAAGGCCTACTGCATCAGACTTGCTGCAGGTACATCTGAATTATTAAGGGGTTTCTTATCACTTGGTCATAAAGGCTTGCGTTTATTTGTGTCCAAGAAAGTAGCTCTATTCTTACCCATTTTCTATGGATATGTGTAGCATCCATTTGTCTCATGTGAGTATCAAGATCCTCATGCAGCAGTCCGTGCCTCTTCTGTGGTCTGTATAATTCCTGACAACTTTGTAACTTTCGTTTTTTTTTTTGGGGGGGGGGGGGGGTGGTTGAAAAGAGTTTGTAACTTTAGTTTCTTTAATGTTTTTATCATCCATCTCAACATCATTATTATTGCAGGAATCTGGGAAACAGATGGCTGATTCTGGGTTGAATTCTAAAGATCTGTAAGGATTTCAATTTATGATAGTTTAATTTCTGATTTTAGTTGATGATTTATTGCCCACTATCAAAATTGTGGACTCTAGACCATTATGTTCATGCTGCAGAACAAATTCTACTATCCAACGTTCAACCTGTGTGGGCTTAAAGGATATTTGTGAAATGGGTAGCTTGAGGTGCTCTTCTGTATATTCTGGGAATTATGGGTCACGGTCCAATTGGGGCTCGAGCAATTTTGAGGATGACATGTGTCAGATAGATGATAAAGATCTGTTGGTTAGTTCATCAATAAAGTACAGATCTATGATATCATCAAATGATTTAAATAAGGTATATTTTGATTCTCTGATTATGTTGTTATTGTTTTTAATAACACCAATAAGAATGGGCAGCTTTTCATTTGAACCAGAGTTTCAATCCTATGTGTGAACCCACTGATGACTGGGCATGTAAATTGGATGAGAGCTCAGAGTTGGGAGGCAATTTAATGGACTTGTCTGGCATCCAAACTAGCCAGGAAACTACTTGTAAGGAGACTGGCTTCAGTTTTCCATGTGGTCAATCAGCAGCTGAGGATGATGAAGAGGTCACGGAGTCAAAGATTAGAGCCTTCCTGGATGAAAAGGTACTTCCATTCTCCAAGATATTTCTGAGTTATGAAATTATTATTTCCTTTTTTAAGCCTAAATAATGCAGCATGATGTGTTTTACATTAGAATTAATGTTTCTACTGAATAAAATGTTAGGCTGTTGAACTGAAGAAGCTGCAAACACCTTTATACGAAGAGTTCCGAAGCACAATTAATGCAGCTAATGCTTTGGGAACAGTTGGAACTGAGCAAGGCAAAAATGTATCTAATTTTTTGAAATTGCCTCCCAAATGTGGGTCACCAAGTAAGCTGCGTGGTAAAAGACTCTCATCAATTGATGCTGGTAACTGCTCCGTTCGTCAGAGTCGCATAAAACAAGCTAGCATACTGCAAGATCGAGCCCTGCAGGAAATTCAGTCACCTCAGCTTGGTGAATGGAACAAATTGCTTCACACGCAGTCGGATTCTTGCACACCAAGGTTGTAATTTAGCAGCTTAAATTTCTGTTTATTTGTGGTAATTTTATTTAACAGCCACAGAATGGAAAACTTGCTTGCAGTTTCTCCGAGAGACAAAGGAAATGGAAAGAAGAGCTTGAGGAAGAGCTCAAAAGGAAGCGAGGTAAGTTCACTTTGCTTGAGAAAAAAGATAATATTATATAGAAAGCAACCAAATGAAACAAACAGCTGCTGCTTATTTAGATATTGATTCGCTTCATTGAAAAATGAGCATTTATATTATCTTGTTAAATGTCATGATACAAGTGTATTTGCTGCATATCTCGTTGCATATTATATACGGGGGAGGTGTGATGATGGAAATGTCATGCATATCGCAGAAATGATGCGCAGAGGACTTGTAGGAGCAATTCACTAAAACCATCATCTGCAAAGGATCAGAGAGTAAAAAATCAACAAAAAGCATGGTGAAGCTCACATGGCCTGGCAGAATAATATTGTGTAGGCATAGTACATGCAGGGGATAAAAGTGCCTTCTCATAGTTGCAATTGTTAGAAATGTATGTACACTATCACCAGCAGAGCTCAGTGTAAGGTGGGGGTGTGCTCCACATCCAGTTCATGGTCTCAGCACAGAGCACAATCTCACAGGGCAGTTGAGGGCATTTCAACTGGAAAAATCTGATAAGTCATATCTTCATATTTCTTTCATTTGATTATTAACATTTGCCTCCCCCTCCCCACGCCCAGTGGTGTGGTTCTATTATCTTCTATGTTATTGGTGGAAATTTGTGACTTAGAAAAAATCTTTTATTTGATGTTCCACAAGTGTGAATCGTTCAAGTTCAATACAAAGGAGATTAGAGTTTATTATAAGTGCCTTTTTTTTTTTACAATATCACAAATTGGAGGGCGGGAGATTCGAACTCACGACTTTTAGAGAAAGTAAAAATGCTTAATGTTGAATTATACTCATGTTGGCAAGTGTCCTTCTGTATATTGCCTCGCTGCTATCTTTGATAAAAATATTAATCTTGATATTGGTTTCTATTGAATTTCTTCGTACAAATACCTCAGAAAAACTTATTCAAATGCCATTATTGCAGTTTGTATTACATGATTTGGTGGGCTGATTTTGGCAGTGTGAAGAATCTTCTGACTTCCCATGTGAAAATTGCTTTTGTTATGTTCTTTTGACATATTAGTTGAATCCTAAATCACCTTTTTTCCTTTCTGGGATTTGAATAATTCATGTCAAAGACGACAATGCTTCAATGAATGTTCCATCTTTTTGTCCACTTGAATCCAAGGAATAGTGATTTGATTTGATCTGAATCGTATGATGAATGTGGGTAATGAAAGTGGTGGTCGGTTCATTGGCCCAACACAGCAATTATGAAGGCGCCATAAGATTTAAGAATTCTGGTTGTGTTTGTGTTTGTGTTTTTGATGTGTTTCACGGCAAAACGGACCATTTAGGGCCCCTCTAATCCCCTTTATGAAGATCCTCAAAAAACACCCCTTTTGAAATTTTGTCGTACACGTCAGCTTAATAGACAGGCTTTCTTTTGGTCAAAAACTTTTTTGGTTTTGAGATGAAATATAGGGTGCAGTTAAAATGAAGGGAAAAAAATTATGAAGACTAATAAAATTGACTAAATCGAGTGATTTTCATCATTCTTAACAAAGACTGTCGGTTTGGTTTGGATTTATTTTATGGATTTGGTGGTTAATTTGTCGTTTATTTTATGGATCACCAATTCAATTTTTAAAACATCAACTTTGATGTTGATTTCATTTTTCATAATTGATAAGTAGATTCAACACCGATTTTGCCTAAAAACTAACTAATTTGATCCATTACACCCCTACAAAAACATAGTAAAAAGTTATAAAAAACTTAATTTTAGTTCATCAAATTTACCTAAGTTTTAATTTTATCAATTTAGACAACTCAATTGAAGGTATTGAAACATATACCTTCAATTGAGTTTCCATCATTTAAATATTTGTGGTTAGTTGCGGCATTGTGACACAAAATTCTTTTTTGTTGGTTCTTTTTAATTTTTTATTAAAATATAGATATATGTATAGATATTAATGGAAAAATAAATAGTTATCTTTAATGTGAGACATCTGGTCCTTGATTATTGGAAAAACAAGGCAATGTTTTCAGATTTTAGAAAAAGGGAAAAGACAATATTCATAATTAAAAAAAAAAAAAAAAAAACATAAAATATATAAAGATATTTGTGTTTGTCATAGAATTAAGGGCAAAGTGTGAAGGGAATGGAAAATGGAGCATTTAGTGGGTTTTCATTTTCCCTTTTTATTTTAAAGAAAAAGCCTCTTAATAAAAAGGTCTTCCAATCCGATCCTTCTTTTTTGTGCTTTACAATTTCATTGGTGTATTGTCAGTCAACATTCATTATTCATCTTATATGCTTTATTTGTTTTTTCATTTCCCATTTGGGATAAACAGAAATACTAATATAATATATATGGTTTATAATATATTATTATTTATTTAATCATGCATTAAAACACTATTTTCATCCAGGATAATAAGGGTTATATGAGAGTTGTGACTCTTCACGATGGAAGTTATTATAGAAAGATTATTGTAACTCTTCCGTATGACTTTTCAATGATAGTAAAATTACCGTAGAAAGATTGTAACTATTTTTGGAATAACATCTGAAAGTCGTACTTTTTTATAGAAATTAAACTCTTCATTTCTCAAGCACATAGTTTTAATAACAATGGATTAGAATATAATTATTGTGTGCAATTTAATTTAAGGGGATGACTCACACTTAATGAGATAATGAAAGAAATAGTAATAAATTAGGATAAATATTTAAGGAGAAGGGAAACATTTCATGCTGTTAAAATCATTGATATGTCATTATGTGGTAAAATATATCACTATATATATATATATAGTATCACTGATATACATTTATGTGATAAAATGTTTTATTGATACTTATATATAACATATCATGATATACATTTTTGAATTATCAATAAAGTATATTACTGATATACTTGATATAAAGCATATCACCGATATACCTAAGAGTATTTTAGACTTTTTATATATTCTGTCTGGGCTAATTTTTTTGCTATACTTGCGATTTTTATAATGTGTGTGCTAGATTTGCTATTTATTTGATTTTTTTGTCAAATTTGCAAGTGCCCTATAATTTAAACCTAAACTTAATTAGTTATCATAAAATAAAACAAAACTATTAAATAAAAATACAAAGAAATTAATAATTAAAAGAGGAGTTCATCGAAGAACGAAGTAAAAAAAAAAATTATATATTTGTTATATTTAATTTTTAGTAGAAAAATCATAATTTTATAATTTAATAGTGAAAAATTTGCTAAAAATAGAAAAAAAAAGAATTTTGGGGGCTAGTTCCAACAAGGTCTGGCCCCTAGAAGTGAGATTCAAACCTTTGACATACACTGAAGTTATTAATGTTTCACTAGTTGAACTATATTTAAGTTGATAAATATAGTCTTTTTACAATACAGATTTTAGTTGATATACTAACTAATCAATCAATCAAAATAATTATATTCAAATCATTAATTCACTAGTATATATGATGTTAATTAAATTTTATTTCAATCATTTTTACAATTTTATAGTTGGACCTACTATTTTTAAAAAGAAAAAATTTTACATTTTAATAAAGTAATTTATGCAACTATCTCTTATTATTTTTATTTTTATTTATTTATTATTATTTTTTTTTTTGCTGTGGAATGCAAAATATCTAACATTATGGGCTACCTTTTGTCAAATAACATTTTACACGTGGAGATTCATTACTGGTCTGTAATCGAGGAGTGGATTGGAGCAAATAAAAATAGCTTCGTGGACAGCATTTGCCAAAAAAAACTTTGATTGAGAGAAATAATTGAAAGATTTTATTGGGAAAAAAAATTAGAAAAATTCAAATGCTCTCTCATAATTCATAAAATAAAACAATCCCAAATCTATTGCCTTTTTCCCCTAAAGCTTTTTACATTGCATCACCAACTTTGTCTTTCACTCTAACATGATCAACATTTTGTTTTGTTTTTATTTCCTTCTTGATTTTTATGGGTCCCCTATATCTTCCTCAACCACGTGCTCCATGTTCGTTAACACGAAGAAAATTTCAAAATCCCGTAAAATACTTTGTACTACTATCACCTATATTATACGCATCACCAATATTTGAAAATAACCTTAGGTCCACTTATTAGTACAATATTATCGTAGAAATATAAAAATTCATAGAATGTCAAATCATCCTTTGATCAACTCATTCTACTATATATTTGTCAATCAGCAACTTCTGACTTTGTAAATGTAGGAAGAATGAACTCTATGATATCGTAGCTTCAATAATCTCGAGACAAGTAGGTGTGGAGTAATATTTTTTAAAAAATAGGGGAATTTGAAATTTCCCAAAGCCCAAGATTGAATTTTTTAGAAAAATGATTTTTAAAAAATGAAAAGAAGTTGTGAAGGATGGAGTTGGCTGGCAGAGATGTATCACCCTATCTCTTAGAATTCTCAATGAATGGATGCCCTAATTCCACGATCTAAACTTCTCTTCCTTTCATCAAGCTCTCCCTCCAATACCATTCCTTATATTTTCTCACATTTCTAATTTTCTCTACCACGTGGTTTTTTTCAATAAAAAAAATGGTTATCTTTAAGATATTTTTTGTTTTTTTTATTAATTGTTGGAGATGAGAATCTGTAGCGAGATCTCAATATATTAATAAAAAAAGGAGAATCATAATAGAGTTCGAAATAAATCAATAGAACTTAAAAGATAAAGCTATAAATATTGTCTAATATATATAAGAATGACAAAGATTCGAGAAGGCTATCACTAAATAAGTTAAACAAACTTGGGCTCCTAGAAAATTGGCATTCCCATGTAACTTTCATATTATATATGATGTTCTATGTAAAATAATCATGAGTTTTTTTTTAATATAATGTAATATTATAAATGTTGACGTGAGAATTTGAATATTCGACTTAGAACAGGTTATTAATGTCTCAATCAATTGAACTATGCTCAAGTTTTCTTCTAAAATAATGGGATTATTAAAAACCAATTGGAACTTGAATTGGCCACAATCATCAAGGGCCAATGCACAAAACCACATTTTTCGATGAATTTTTTTATATATCGTGATGGAAAGCCTAACTTATAGTTTTTGTAGAAAGTTAAAATTGTAGTAGTTTGAAAGATATAATTGGATTATAGATTAAACTAAGCTACAAATTATTTTTGATGGAAATGTAATTATAGATATTGTCTCTCTTTTTTTTTTAAAAAAAAGGTACTATTTGAATTTTTGTATTTAGCACCAAAATTACAAAAAAAAAAAAAAAAAAGAATTTTGATTTGATGTTAGATTGCTTTCGATCTCTTGAGCTTATTTGAAGTTGACACATTTTTTAAAAAAAAATGTACAAATTGAGAAAGATAAATATGAATTCGAATATACTATTTTAAATCGGATGAACTTTGTTGGTTATTATCTAAAATGGTGAAGTTGACTTTTAATTTTAAATGTTGTGAAATAAAAAAATAATTACGACAAAATATATATTTATATACCTTCTTGGTCTACATATTTTCTCGTGCAAATTTTAATGGTTCATATTTAGTTTGTCTTAAATAGAAGTATAAATGAATATATGAAACAAAAAATATATTATATACTCATCTTTATATGGATTATAAAATATCATAAAGCATTAAAATTTTCTTTTAAAAAAGTTTGTTAAATTAAAAGAAAATTAAAAAGATGACAGAAAAAATGTTGTTATCGTAATTTTGCCACTTGGAGAAGGAGAATAGGCAGAAGCTTCCTTTTCATTATCTACTTTGCCTCTCAAAATAGACAAAAGAAAATAACATCATTTTCATCACAATCAATTTATGGAATTGCATATAATTTTTCAAAAATTCTAACTCATTCAATTTAAATCTTCATCTTCTAATTTGATTCAAGTGGGATTAATTTCTTTAACATAAGACAATATTACAAATAAAATTATGTGACTTGTCTAAAATGATTAAAAAAATCAAGCTTCTATATATTTTAGTGCATCTTGATATTTTTTTTTCTAATAATTCCATAAACTACATAATAATTGTTTGTGAAATTTTCATTTTTATCCTAATTTGTACTTAACTAAATAAGTCGAATCTAGTCTTAAATTTTTAATTTCATCTAATTGAATTCTAAATTTAAATAAGCAGTAAATTAATAGTTCCACTCTAATTTAGTTGGATTTCTATTTTTGTAAAGATTTATAATGATAAGTTCATGAACAAATTTTTAATTTAGTTTTAAAAGAAATATTAATTTTATTGTTGGTTGAAGTTTTTTTTTTAGTTTAACAAGGATACGGGTGAAGGATTAAACTTTCAATCTCATAAAAATAATAAGTATCTTATCTACTAAAACTATACTCAAATTGACACATTATTGGTTGAAGTTCACAGTTCGATCAAATCAAAATTTAAAAATTAAAATCGACGGGTTAAAATATTTTGTCTTAATTAATTATTATTTTATTTTTCGATGAGAGAGAGAGAGAAATAATGGCTATGAGTCTATCGCGTAGCACAGACTACGAGACAAGGGAATTTTAACAAAAAGGATGGAACGGACAAATGAGGATAAAGTCAAAACAAAATGTTGTCCAATTTTAATTACACCAACTTTTTTTTTCAGTCATTAGTTTTAACTTTTAAGTTATTTAATGTCAGAACCTTTTCCAAAGAAAAACAATCTTTTATTGTACCAGGTTGATTTTTAGAATTTAAAATATATTAAATTACAAAATTTAGATATTTATTTAGTTTTAAAAATGTTTAAATATGATTGTGTTGAGTCAATTGATATATTGATTGGACTGATAATTTAGCATGACATAGAGAATAAATTAGCAAATTTATATCAAAATTAAAAGACGAGATTATAATATTTGATATTATAAGGATGAAAAGGAAATAAATGTAAAGTTTTGGGGTGAAAAGTAGAAACCAAACAAACTTTTTGATTTTTGTTTTTTGAATTAACAAGTAGGGATGCTAAATTGGTCCAAATTTATGAGCCCATTGTTCTTGCTGTTTCCTTCTTGTTTCATGGTGCATGCCTTTTCGTCGTTCGTCTCCGTCGTCGCTGTTTCGGCTCCGATCTCTTCTTCGCCGTAGTTGATTTCTTCATTTTCCTCCGGCTCCAAAAAGCAGAGCCGGAGGCGGCCATGGCTTCGCTCCGCTTGGAAACAAGACTGCCTCGCCGGAGTTTTCACCGCCTCGATTATTAG

mRNA sequence

ATGCAAGATTTCTTTGGATCCGTTCGCCGATCCATAGTATTTCTAGCTCCCGACGGAGACGACGGAGGGAGGTTTGGTGGCCTGGTCGAGAAGATCGGCTCGAGCATCCGTAAATCCCGAAATGGGCTGTTTTCTAAGCATTCAGTACGGGCTCTGCCTCCAGTGGCGAAGGAAGATTCACCTCCGATTTGGTGGCGGAAGGGGGAGTTGATCGGATGCGGTGCTTTTGGTCGAGTTTATATGGGGATGAACCTTGATTCTGGAGAGCTTCTTGCAGTCAAACAAGTCTCAATTGCTGCCAATAGTGCTTCAAGGGAGAAAGCACAAGCTCACATACGGGAGCTGGAGGAGGAAGTGAAGCTTTTGAAGAATCTTTCTCACCCCAATATTGTGAGATATCTGGGAACTGCCAGAGAGGAAGAGTCATTAAATATTCTATTGGAGTTTGTTCCTGGAGGATCAATATCATCTTTGTTAGGGAAATTTGGATCTTTCCCCGAGTCTGTTATAAGAACCTACACAAAGCAGCTATTATTGGGACTTGAGTACCTTCATAAGAACGGCATTATGCATAGGGATATAAAGGGTGCAAACATTCTTGTAGACAATAAGGGGTGTATTAAACTCGCAGATTTTGGTGCATCCAAGAAAGTGGTTGAGCTGGCAACTATAAATGGAGCCAAATCAATGAAGGGTACTCCATATTGGATGGCTCCTGAAGTTATTCTCCAGACTGGACATAGCTTCTCTGCTGATATATGGAGTGTGGGGTGTACTGTTATTGAAATGGCAACAGGGAAGCCTCCATGGAGCCAACAGTATCAAGAGGTTGCTGCTCTTTTCCATATAGGGAATACTAAGTCCCATCCGCCCATTCCTGAGCATCTATCTGCTGAGGCAAAAGATTTTCTGTTAAAATGTTTACAAGAGGAGCCAAACTCAAGGCCTACTGCATCAGACTTGCTGCAGCATCCATTTGTCTCATGTGAGTATCAAGATCCTCATGCAGCAGTCCGTGCCTCTTCTGTGGAATCTGGGAAACAGATGGCTGATTCTGGGTTGAATTCTAAAGATCTAACAAATTCTACTATCCAACGTTCAACCTGTGTGGGCTTAAAGGATATTTGTGAAATGGGTAGCTTGAGGTGCTCTTCTGTATATTCTGGGAATTATGGGTCACGGTCCAATTGGGGCTCGAGCAATTTTGAGGATGACATGTGTCAGATAGATGATAAAGATCTGTTGGTTAGTTCATCAATAAAGTACAGATCTATGATATCATCAAATGATTTAAATAAGAGTTTCAATCCTATGTGTGAACCCACTGATGACTGGGCATGTAAATTGGATGAGAGCTCAGAGTTGGGAGGCAATTTAATGGACTTGTCTGGCATCCAAACTAGCCAGGAAACTACTTGTAAGGAGACTGGCTTCAGTTTTCCATGTGGTCAATCAGCAGCTGAGGATGATGAAGAGGTCACGGAGTCAAAGATTAGAGCCTTCCTGGATGAAAAGGCTGTTGAACTGAAGAAGCTGCAAACACCTTTATACGAAGAGTTCCGAAGCACAATTAATGCAGCTAATGCTTTGGGAACAGTTGGAACTGAGCAAGGCAAAAATGTATCTAATTTTTTGAAATTGCCTCCCAAATGTGGGTCACCAAGTAAGCTGCGTGGTAAAAGACTCTCATCAATTGATGCTGGTAACTGCTCCGTTCGTCAGAGTCGCATAAAACAAGCTAGCATACTGCAAGATCGAGCCCTGCAGGAAATTCAGTCACCTCAGCTTGGTGAATGGAACAAATTGCTTCACACGCAGTCGGATTCTTGCACACCAAGTTTCTCCGAGAGACAAAGGAAATGGAAAGAAGAGCTTGAGGAAGAGCTCAAAAGGAAGCGAGCAGAGCTCAGTGTAAGGTGGGGGTGTGCTCCACATCCAGTTCATGGTCTCAGCACAGAGCACAATCTCACAGGGCAGTTGAGGGCATTTCAACTGGAAAAATCTGATAAGGATGCTAAATTGGTCCAAATTTATGAGCCCATTGTTCTTGCTGTTTCCTTCTTGTTTCATGGTGCATGCCTTTTCGTCGTTCGTCTCCGTCGTCGCTGTTTCGGCTCCGATCTCTTCTTCGCCGTAGTTGATTTCTTCATTTTCCTCCGGCTCCAAAAAGCAGAGCCGGAGGCGGCCATGGCTTCGCTCCGCTTGGAAACAAGACTGCCTCGCCGGAGTTTTCACCGCCTCGATTATTAG

Coding sequence (CDS)

ATGCAAGATTTCTTTGGATCCGTTCGCCGATCCATAGTATTTCTAGCTCCCGACGGAGACGACGGAGGGAGGTTTGGTGGCCTGGTCGAGAAGATCGGCTCGAGCATCCGTAAATCCCGAAATGGGCTGTTTTCTAAGCATTCAGTACGGGCTCTGCCTCCAGTGGCGAAGGAAGATTCACCTCCGATTTGGTGGCGGAAGGGGGAGTTGATCGGATGCGGTGCTTTTGGTCGAGTTTATATGGGGATGAACCTTGATTCTGGAGAGCTTCTTGCAGTCAAACAAGTCTCAATTGCTGCCAATAGTGCTTCAAGGGAGAAAGCACAAGCTCACATACGGGAGCTGGAGGAGGAAGTGAAGCTTTTGAAGAATCTTTCTCACCCCAATATTGTGAGATATCTGGGAACTGCCAGAGAGGAAGAGTCATTAAATATTCTATTGGAGTTTGTTCCTGGAGGATCAATATCATCTTTGTTAGGGAAATTTGGATCTTTCCCCGAGTCTGTTATAAGAACCTACACAAAGCAGCTATTATTGGGACTTGAGTACCTTCATAAGAACGGCATTATGCATAGGGATATAAAGGGTGCAAACATTCTTGTAGACAATAAGGGGTGTATTAAACTCGCAGATTTTGGTGCATCCAAGAAAGTGGTTGAGCTGGCAACTATAAATGGAGCCAAATCAATGAAGGGTACTCCATATTGGATGGCTCCTGAAGTTATTCTCCAGACTGGACATAGCTTCTCTGCTGATATATGGAGTGTGGGGTGTACTGTTATTGAAATGGCAACAGGGAAGCCTCCATGGAGCCAACAGTATCAAGAGGTTGCTGCTCTTTTCCATATAGGGAATACTAAGTCCCATCCGCCCATTCCTGAGCATCTATCTGCTGAGGCAAAAGATTTTCTGTTAAAATGTTTACAAGAGGAGCCAAACTCAAGGCCTACTGCATCAGACTTGCTGCAGCATCCATTTGTCTCATGTGAGTATCAAGATCCTCATGCAGCAGTCCGTGCCTCTTCTGTGGAATCTGGGAAACAGATGGCTGATTCTGGGTTGAATTCTAAAGATCTAACAAATTCTACTATCCAACGTTCAACCTGTGTGGGCTTAAAGGATATTTGTGAAATGGGTAGCTTGAGGTGCTCTTCTGTATATTCTGGGAATTATGGGTCACGGTCCAATTGGGGCTCGAGCAATTTTGAGGATGACATGTGTCAGATAGATGATAAAGATCTGTTGGTTAGTTCATCAATAAAGTACAGATCTATGATATCATCAAATGATTTAAATAAGAGTTTCAATCCTATGTGTGAACCCACTGATGACTGGGCATGTAAATTGGATGAGAGCTCAGAGTTGGGAGGCAATTTAATGGACTTGTCTGGCATCCAAACTAGCCAGGAAACTACTTGTAAGGAGACTGGCTTCAGTTTTCCATGTGGTCAATCAGCAGCTGAGGATGATGAAGAGGTCACGGAGTCAAAGATTAGAGCCTTCCTGGATGAAAAGGCTGTTGAACTGAAGAAGCTGCAAACACCTTTATACGAAGAGTTCCGAAGCACAATTAATGCAGCTAATGCTTTGGGAACAGTTGGAACTGAGCAAGGCAAAAATGTATCTAATTTTTTGAAATTGCCTCCCAAATGTGGGTCACCAAGTAAGCTGCGTGGTAAAAGACTCTCATCAATTGATGCTGGTAACTGCTCCGTTCGTCAGAGTCGCATAAAACAAGCTAGCATACTGCAAGATCGAGCCCTGCAGGAAATTCAGTCACCTCAGCTTGGTGAATGGAACAAATTGCTTCACACGCAGTCGGATTCTTGCACACCAAGTTTCTCCGAGAGACAAAGGAAATGGAAAGAAGAGCTTGAGGAAGAGCTCAAAAGGAAGCGAGCAGAGCTCAGTGTAAGGTGGGGGTGTGCTCCACATCCAGTTCATGGTCTCAGCACAGAGCACAATCTCACAGGGCAGTTGAGGGCATTTCAACTGGAAAAATCTGATAAGGATGCTAAATTGGTCCAAATTTATGAGCCCATTGTTCTTGCTGTTTCCTTCTTGTTTCATGGTGCATGCCTTTTCGTCGTTCGTCTCCGTCGTCGCTGTTTCGGCTCCGATCTCTTCTTCGCCGTAGTTGATTTCTTCATTTTCCTCCGGCTCCAAAAAGCAGAGCCGGAGGCGGCCATGGCTTCGCTCCGCTTGGAAACAAGACTGCCTCGCCGGAGTTTTCACCGCCTCGATTATTAG

Protein sequence

MQDFFGSVRRSIVFLAPDGDDGGRFGGLVEKIGSSIRKSRNGLFSKHSVRALPPVAKEDSPPIWWRKGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRELEEEVKLLKNLSHPNIVRYLGTAREEESLNILLEFVPGGSISSLLGKFGSFPESVIRTYTKQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEAKDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVESGKQMADSGLNSKDLTNSTIQRSTCVGLKDICEMGSLRCSSVYSGNYGSRSNWGSSNFEDDMCQIDDKDLLVSSSIKYRSMISSNDLNKSFNPMCEPTDDWACKLDESSELGGNLMDLSGIQTSQETTCKETGFSFPCGQSAAEDDEEVTESKIRAFLDEKAVELKKLQTPLYEEFRSTINAANALGTVGTEQGKNVSNFLKLPPKCGSPSKLRGKRLSSIDAGNCSVRQSRIKQASILQDRALQEIQSPQLGEWNKLLHTQSDSCTPSFSERQRKWKEELEEELKRKRAELSVRWGCAPHPVHGLSTEHNLTGQLRAFQLEKSDKDAKLVQIYEPIVLAVSFLFHGACLFVVRLRRRCFGSDLFFAVVDFFIFLRLQKAEPEAAMASLRLETRLPRRSFHRLDY
Homology
BLAST of Sgr016133 vs. NCBI nr
Match: XP_022138818.1 (mitogen-activated protein kinase kinase kinase NPK1 [Momordica charantia])

HSP 1 Score: 1192.6 bits (3084), Expect = 0.0e+00
Identity = 596/634 (94.01%), Postives = 611/634 (96.37%), Query Frame = 0

Query: 1   MQDFFGSVRRSIVFLAPDGDDGGRFGGLVEKIGSSIRKSRNGLFSKHSVRALPPVAKEDS 60
           MQDFFGSVRRS+VFL PDGDDGGRFGGLVEKIGSSIRKSRNGLFSKHSVRALPPV KED+
Sbjct: 1   MQDFFGSVRRSLVFLTPDGDDGGRFGGLVEKIGSSIRKSRNGLFSKHSVRALPPVMKEDA 60

Query: 61  PPIWWRKGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRELEEEVK 120
           PPIWWR+GELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRELEEEVK
Sbjct: 61  PPIWWRRGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRELEEEVK 120

Query: 121 LLKNLSHPNIVRYLGTAREEESLNILLEFVPGGSISSLLGKFGSFPESVIRTYTKQLLLG 180
           LLKNLSHPNIVRYLGT R E+SLNILLEFVPGGSISSLLGKFGSFPESVIRTYTKQLLLG
Sbjct: 121 LLKNLSHPNIVRYLGTTRGEDSLNILLEFVPGGSISSLLGKFGSFPESVIRTYTKQLLLG 180

Query: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240
           LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE
Sbjct: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240

Query: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300
           VILQTGHSF ADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA
Sbjct: 241 VILQTGHSFPADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300

Query: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVESGKQMADSGLNSKDLT 360
           KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVESGKQM  SGLNS DL 
Sbjct: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVESGKQMVGSGLNSNDLA 360

Query: 361 NSTIQRSTCVGLKDICEMGSLRCSSVYSGNYGSRSNWGSSNFEDDMCQIDDKDLLVSSSI 420
           NSTIQRSTCVGLKDICEMGSLRCSSVYSGNY S+SNWGSSNFE+DMCQIDDKDLLVS SI
Sbjct: 361 NSTIQRSTCVGLKDICEMGSLRCSSVYSGNYTSQSNWGSSNFEEDMCQIDDKDLLVSPSI 420

Query: 421 KYRSMISSNDLNKSFNPMCEPT-DDWACKLDESSELGGNLMDLSGIQTSQETTCKETGFS 480
           KY+S+ISS+DLNKSFNPMCEPT DDWACKLD SSELGGNLMDLSGIQTSQETTCKE  F+
Sbjct: 421 KYKSIISSSDLNKSFNPMCEPTDDDWACKLDGSSELGGNLMDLSGIQTSQETTCKENDFT 480

Query: 481 FPCGQSAAEDDEEVTESKIRAFLDEKAVELKKLQTPLYEEFRSTINAANALGTVGTEQGK 540
           FPCGQSA EDDEEVTESKIRAFLDEKA+ELKK+QTPLYEEFRSTINAANALG VGTE  K
Sbjct: 481 FPCGQSAVEDDEEVTESKIRAFLDEKAIELKKMQTPLYEEFRSTINAANALGAVGTENSK 540

Query: 541 NVSNFLKLPPKCGSPSKLRGKRLSSIDAGNCSVRQSRIKQASILQDRALQEIQSPQLGEW 600
           +VSNFLKLPPKCGSPSKLRGKRLSSIDA NCS RQSRIKQASIL+DRALQEIQSPQLGEW
Sbjct: 541 SVSNFLKLPPKCGSPSKLRGKRLSSIDASNCSSRQSRIKQASILEDRALQEIQSPQLGEW 600

Query: 601 NKLLHTQSDSCTPSFSERQRKWKEELEEELKRKR 634
           NKLLHTQ +S TPSFSERQRKWKEELEEELKRKR
Sbjct: 601 NKLLHTQPESSTPSFSERQRKWKEELEEELKRKR 634

BLAST of Sgr016133 vs. NCBI nr
Match: XP_023547660.1 (mitogen-activated protein kinase kinase kinase NPK1 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023547661.1 mitogen-activated protein kinase kinase kinase NPK1 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023547662.1 mitogen-activated protein kinase kinase kinase NPK1 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1183.3 bits (3060), Expect = 0.0e+00
Identity = 586/633 (92.58%), Postives = 610/633 (96.37%), Query Frame = 0

Query: 1   MQDFFGSVRRSIVFLAPDGDDGGRFGGLVEKIGSSIRKSRNGLFSKHSVRALPPVAKEDS 60
           MQDFFGSVRRS+VFLAPDGDDGG+FGGLVE+IGSSIRKSRNGLFSKHSVRALPPVAKED+
Sbjct: 1   MQDFFGSVRRSLVFLAPDGDDGGKFGGLVERIGSSIRKSRNGLFSKHSVRALPPVAKEDA 60

Query: 61  PPIWWRKGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRELEEEVK 120
           PPIWWRKGELIGCGAFGRVYMGMNLDSGEL+A+KQVSIAANSASREKAQAHIRELEEEVK
Sbjct: 61  PPIWWRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHIRELEEEVK 120

Query: 121 LLKNLSHPNIVRYLGTAREEESLNILLEFVPGGSISSLLGKFGSFPESVIRTYTKQLLLG 180
           LLKNLSHPNIVRYLGTAREE SLNILLEFVPGGSISSLLGKFGSFPESV++TYTKQLLLG
Sbjct: 121 LLKNLSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKTYTKQLLLG 180

Query: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240
           LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE
Sbjct: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240

Query: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300
           VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA
Sbjct: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300

Query: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVESGKQMADSGLNSKDLT 360
           KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQ+PHAAVRASS+ESGKQMADS LNS D+T
Sbjct: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQEPHAAVRASSLESGKQMADSRLNSNDIT 360

Query: 361 NSTIQRSTCVGLKDICEMGSLRCSSVYSGNYGSRSNWGSSNFEDDMCQIDDKDLLVSSSI 420
            S IQRSTC GLKDICEMGSLRCSSV+SGNY  RSNWGSSNFEDDMCQIDDKDL  SSSI
Sbjct: 361 KSIIQRSTCEGLKDICEMGSLRCSSVFSGNYEVRSNWGSSNFEDDMCQIDDKDLFASSSI 420

Query: 421 KYRSMISSNDLNKSFNPMCEPTDDWACKLDESSELGGNLMDLSGIQTSQETTCKETGFSF 480
           KY+S+ISSNDLNKSFNPMCEPTDDWACKLDESSEL GNLM+LSGIQT QE+T K   F+F
Sbjct: 421 KYKSIISSNDLNKSFNPMCEPTDDWACKLDESSELRGNLMELSGIQTGQESTSKGNDFTF 480

Query: 481 PCGQSAAEDDEEVTESKIRAFLDEKAVELKKLQTPLYEEFRSTINAANALGTVGTEQGKN 540
           PCGQS AEDDEEVT+SKI+AFLDEKA+ELKKLQTPLYEEFRST+NAANALG  GTE  K+
Sbjct: 481 PCGQSTAEDDEEVTDSKIQAFLDEKALELKKLQTPLYEEFRSTVNAANALGAGGTENSKS 540

Query: 541 VSNFLKLPPKCGSPSKLRGKRLSSIDAGNCSVRQSRIKQASILQDRALQEIQSPQLGEWN 600
           VSNFLKLPPKCGSPSKLRGKRLSSIDAGNCS RQSRIKQASILQDRALQEIQSPQ+GEWN
Sbjct: 541 VSNFLKLPPKCGSPSKLRGKRLSSIDAGNCSSRQSRIKQASILQDRALQEIQSPQIGEWN 600

Query: 601 KLLHTQSDSCTPSFSERQRKWKEELEEELKRKR 634
           KLLHTQ +SCTPSFSERQRKWKEELEEELKRKR
Sbjct: 601 KLLHTQPESCTPSFSERQRKWKEELEEELKRKR 633

BLAST of Sgr016133 vs. NCBI nr
Match: XP_023006461.1 (mitogen-activated protein kinase kinase kinase NPK1 isoform X2 [Cucurbita maxima])

HSP 1 Score: 1181.8 bits (3056), Expect = 0.0e+00
Identity = 584/633 (92.26%), Postives = 611/633 (96.52%), Query Frame = 0

Query: 1   MQDFFGSVRRSIVFLAPDGDDGGRFGGLVEKIGSSIRKSRNGLFSKHSVRALPPVAKEDS 60
           MQDFFGSVRRS+VFLAPDGDDGG+FGGLVE+IGSSIRKSRNGLFSKHSVRALPPVAKED+
Sbjct: 1   MQDFFGSVRRSLVFLAPDGDDGGKFGGLVERIGSSIRKSRNGLFSKHSVRALPPVAKEDA 60

Query: 61  PPIWWRKGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRELEEEVK 120
           PPIWWRKGELIGCGAFGRVYMGMNLDSGEL+A+KQVSIAANSASREKAQAHIRELEEEVK
Sbjct: 61  PPIWWRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHIRELEEEVK 120

Query: 121 LLKNLSHPNIVRYLGTAREEESLNILLEFVPGGSISSLLGKFGSFPESVIRTYTKQLLLG 180
           LLKNLSHPNIVRYLGTAREE SLNILLEFVPGGSISSLLGKFGSFPESV++TYTKQLLLG
Sbjct: 121 LLKNLSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKTYTKQLLLG 180

Query: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240
           LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE
Sbjct: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240

Query: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300
           VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA
Sbjct: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300

Query: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVESGKQMADSGLNSKDLT 360
           KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQ+PHAAVR SS+ESGKQMADS LNSKD+T
Sbjct: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQEPHAAVRVSSLESGKQMADSRLNSKDIT 360

Query: 361 NSTIQRSTCVGLKDICEMGSLRCSSVYSGNYGSRSNWGSSNFEDDMCQIDDKDLLVSSSI 420
            S IQRSTC GLKDICEMGSLRCSSV+SGNY  RSNWGSSNFEDDMCQIDDK+L  SSSI
Sbjct: 361 KSIIQRSTCEGLKDICEMGSLRCSSVFSGNYEVRSNWGSSNFEDDMCQIDDKNLFASSSI 420

Query: 421 KYRSMISSNDLNKSFNPMCEPTDDWACKLDESSELGGNLMDLSGIQTSQETTCKETGFSF 480
           KY+S+ISSNDLNKSFNPMCEPTDDWACKLDESSEL GNLM+LSGI+T QE+T K   F+F
Sbjct: 421 KYKSIISSNDLNKSFNPMCEPTDDWACKLDESSELRGNLMELSGIRTGQESTSKGNDFTF 480

Query: 481 PCGQSAAEDDEEVTESKIRAFLDEKAVELKKLQTPLYEEFRSTINAANALGTVGTEQGKN 540
           PCGQS AEDDEEVT+SKI+AFLDEKA+ELKKLQTPLYEEFRST+NAANALG VGTE  K+
Sbjct: 481 PCGQSTAEDDEEVTDSKIQAFLDEKALELKKLQTPLYEEFRSTVNAANALGAVGTENSKS 540

Query: 541 VSNFLKLPPKCGSPSKLRGKRLSSIDAGNCSVRQSRIKQASILQDRALQEIQSPQLGEWN 600
           VSNFLKLPPKCGSPSKLRGKRLSSIDAGNCS RQSRIKQASILQDRALQEIQSPQ+GEWN
Sbjct: 541 VSNFLKLPPKCGSPSKLRGKRLSSIDAGNCSSRQSRIKQASILQDRALQEIQSPQIGEWN 600

Query: 601 KLLHTQSDSCTPSFSERQRKWKEELEEELKRKR 634
           KLLHTQ +SCTP+FSERQRKWKEELEEELKRKR
Sbjct: 601 KLLHTQPESCTPTFSERQRKWKEELEEELKRKR 633

BLAST of Sgr016133 vs. NCBI nr
Match: XP_023006458.1 (mitogen-activated protein kinase kinase kinase NPK1 isoform X1 [Cucurbita maxima] >XP_023006459.1 mitogen-activated protein kinase kinase kinase NPK1 isoform X1 [Cucurbita maxima] >XP_023006460.1 mitogen-activated protein kinase kinase kinase NPK1 isoform X1 [Cucurbita maxima])

HSP 1 Score: 1177.2 bits (3044), Expect = 0.0e+00
Identity = 584/634 (92.11%), Postives = 611/634 (96.37%), Query Frame = 0

Query: 1   MQDFFGSVRRSIVFLAPDGDDGGRFGGLVEKIGSSIRKSRNGLFSKHSVRALPPVAKEDS 60
           MQDFFGSVRRS+VFLAPDGDDGG+FGGLVE+IGSSIRKSRNGLFSKHSVRALPPVAKED+
Sbjct: 1   MQDFFGSVRRSLVFLAPDGDDGGKFGGLVERIGSSIRKSRNGLFSKHSVRALPPVAKEDA 60

Query: 61  PPIWWRKGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRELEEEVK 120
           PPIWWRKGELIGCGAFGRVYMGMNLDSGEL+A+KQVSIAANSASREKAQAHIRELEEEVK
Sbjct: 61  PPIWWRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHIRELEEEVK 120

Query: 121 LLKNLSHPNIVRYLGTAREEESLNILLEFVPGGSISSLLGKFGSFPESVIRTYTKQLLLG 180
           LLKNLSHPNIVRYLGTAREE SLNILLEFVPGGSISSLLGKFGSFPESV++TYTKQLLLG
Sbjct: 121 LLKNLSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKTYTKQLLLG 180

Query: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240
           LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE
Sbjct: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240

Query: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300
           VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA
Sbjct: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300

Query: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVESGKQMADSGLNSKDLT 360
           KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQ+PHAAVR SS+ESGKQMADS LNSKD+T
Sbjct: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQEPHAAVRVSSLESGKQMADSRLNSKDIT 360

Query: 361 NSTIQRSTCVGLKDICEMGSLRCSSVYSGNYGSRSNWGSSNFEDDMCQIDDKDLLVSSSI 420
            S IQRSTC GLKDICEMGSLRCSSV+SGNY  RSNWGSSNFEDDMCQIDDK+L  SSSI
Sbjct: 361 KSIIQRSTCEGLKDICEMGSLRCSSVFSGNYEVRSNWGSSNFEDDMCQIDDKNLFASSSI 420

Query: 421 KYRSMISSNDLNKSFNPMCEPTDDWACKLDESSELGGNLMDLSGIQTSQETTCKETGFSF 480
           KY+S+ISSNDLNKSFNPMCEPTDDWACKLDESSEL GNLM+LSGI+T QE+T K   F+F
Sbjct: 421 KYKSIISSNDLNKSFNPMCEPTDDWACKLDESSELRGNLMELSGIRTGQESTSKGNDFTF 480

Query: 481 PCGQSAAEDDEEVTESKIRAFLDEKAVELKKLQTPLYEEFRSTINAANALGTVGTEQGKN 540
           PCGQS AEDDEEVT+SKI+AFLDEKA+ELKKLQTPLYEEFRST+NAANALG VGTE  K+
Sbjct: 481 PCGQSTAEDDEEVTDSKIQAFLDEKALELKKLQTPLYEEFRSTVNAANALGAVGTENSKS 540

Query: 541 VSNFLKLPPKCGSPSKLRGKRLSSIDAGNCSVRQSRIKQASILQDRALQEIQSPQLGEWN 600
           VSNFLKLPPKCGSPSKLRGKRLSSIDAGNCS RQSRIKQASILQDRALQEIQSPQ+GEWN
Sbjct: 541 VSNFLKLPPKCGSPSKLRGKRLSSIDAGNCSSRQSRIKQASILQDRALQEIQSPQIGEWN 600

Query: 601 KLLHTQSDSCTPS-FSERQRKWKEELEEELKRKR 634
           KLLHTQ +SCTP+ FSERQRKWKEELEEELKRKR
Sbjct: 601 KLLHTQPESCTPTFFSERQRKWKEELEEELKRKR 634

BLAST of Sgr016133 vs. NCBI nr
Match: KAG6575002.1 (Mitogen-activated protein kinase kinase kinase NPK1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7013573.1 Mitogen-activated protein kinase kinase kinase NPK1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1175.2 bits (3039), Expect = 0.0e+00
Identity = 582/633 (91.94%), Postives = 607/633 (95.89%), Query Frame = 0

Query: 1   MQDFFGSVRRSIVFLAPDGDDGGRFGGLVEKIGSSIRKSRNGLFSKHSVRALPPVAKEDS 60
           MQDFFGSVRRS+VFLAPDGDDGG+FGGLVE+IGSSIRKSRNGLFSKHSVRALPPVAKED+
Sbjct: 1   MQDFFGSVRRSLVFLAPDGDDGGKFGGLVERIGSSIRKSRNGLFSKHSVRALPPVAKEDA 60

Query: 61  PPIWWRKGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRELEEEVK 120
           P IWWRKGELIGCGAFGRVYMGMNLDSGEL+A+KQVSIAANSASREKAQAHIRELEEEVK
Sbjct: 61  PSIWWRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHIRELEEEVK 120

Query: 121 LLKNLSHPNIVRYLGTAREEESLNILLEFVPGGSISSLLGKFGSFPESVIRTYTKQLLLG 180
           LLKNLSHPNIVRYLGTAREE SLNILLEFVPGGSISSLLGKFGSFPESV++TYTKQLLLG
Sbjct: 121 LLKNLSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKTYTKQLLLG 180

Query: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240
           LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE
Sbjct: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240

Query: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300
           VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA
Sbjct: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300

Query: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVESGKQMADSGLNSKDLT 360
           KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQ+PHA VRASS+ESGKQMADS LNS D+T
Sbjct: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQEPHAPVRASSLESGKQMADSRLNSNDIT 360

Query: 361 NSTIQRSTCVGLKDICEMGSLRCSSVYSGNYGSRSNWGSSNFEDDMCQIDDKDLLVSSSI 420
            S IQRSTC GLKDICEMGSLRCSSV+SGNY  RSNWGSSNFEDDMCQIDDKDL  SSSI
Sbjct: 361 KSIIQRSTCEGLKDICEMGSLRCSSVFSGNYEVRSNWGSSNFEDDMCQIDDKDLFASSSI 420

Query: 421 KYRSMISSNDLNKSFNPMCEPTDDWACKLDESSELGGNLMDLSGIQTSQETTCKETGFSF 480
           KY+S+ISSNDLNKSFNPMCEPTDDW CKLDESSEL GNLM+LSGIQT QE+T K   F+F
Sbjct: 421 KYKSIISSNDLNKSFNPMCEPTDDWPCKLDESSELRGNLMELSGIQTGQESTSKGNDFTF 480

Query: 481 PCGQSAAEDDEEVTESKIRAFLDEKAVELKKLQTPLYEEFRSTINAANALGTVGTEQGKN 540
           PCGQS AEDDEEVT+SKI+AFLDEKA+ELKKLQTPLYEEFRST+NAANALG  GTE  K+
Sbjct: 481 PCGQSTAEDDEEVTDSKIQAFLDEKALELKKLQTPLYEEFRSTVNAANALGAGGTENSKS 540

Query: 541 VSNFLKLPPKCGSPSKLRGKRLSSIDAGNCSVRQSRIKQASILQDRALQEIQSPQLGEWN 600
           VSNFLKLPPKCGSPSKLRGKRLSSIDAGNCS RQSRIKQASILQDRALQEIQSPQ+GEWN
Sbjct: 541 VSNFLKLPPKCGSPSKLRGKRLSSIDAGNCSSRQSRIKQASILQDRALQEIQSPQIGEWN 600

Query: 601 KLLHTQSDSCTPSFSERQRKWKEELEEELKRKR 634
           +LLHTQ +SCTPSFSERQRKWKEELEEELKRKR
Sbjct: 601 RLLHTQPESCTPSFSERQRKWKEELEEELKRKR 633

BLAST of Sgr016133 vs. ExPASy Swiss-Prot
Match: Q40541 (Mitogen-activated protein kinase kinase kinase NPK1 OS=Nicotiana tabacum OX=4097 GN=NPK1 PE=1 SV=1)

HSP 1 Score: 796.2 bits (2055), Expect = 3.2e-229
Identity = 422/662 (63.75%), Postives = 499/662 (75.38%), Query Frame = 0

Query: 1   MQDFFGSVRRSIVFLAPDGDD------GGRFGGLVEKIGSSIRKSRNGLFSKHSVRALPP 60
           MQDF GSVRRS+VF      D      G  FGG VEK+GSSIRKS  G+FSK  V ALP 
Sbjct: 1   MQDFIGSVRRSLVFKQSGDFDTGAAGVGSGFGGFVEKLGSSIRKSSIGIFSKAHVPALPS 60

Query: 61  VA---------KEDSPPIWWRKGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASR 120
           ++         K+D+PPI WRKGE+IGCGAFGRVYMGMN+DSGELLA+K+VSIA N ASR
Sbjct: 61  ISKAELPAKARKDDTPPIRWRKGEMIGCGAFGRVYMGMNVDSGELLAIKEVSIAMNGASR 120

Query: 121 EKAQAHIRELEEEVKLLKNLSHPNIVRYLGTAREEESLNILLEFVPGGSISSLLGKFGSF 180
           E+AQAH+RELEEEV LLKNLSHPNIVRYLGTARE  SLNILLEFVPGGSISSLLGKFGSF
Sbjct: 121 ERAQAHVRELEEEVNLLKNLSHPNIVRYLGTAREAGSLNILLEFVPGGSISSLLGKFGSF 180

Query: 181 PESVIRTYTKQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATIN 240
           PESVIR YTKQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELAT+ 
Sbjct: 181 PESVIRMYTKQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATMT 240

Query: 241 GAKSMKGTPYWMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGN 300
           GAKSMKGTPYWMAPEVILQTGHSFSADIWSVGCT+IEMATGKPPWSQQYQEVAALFHIG 
Sbjct: 241 GAKSMKGTPYWMAPEVILQTGHSFSADIWSVGCTIIEMATGKPPWSQQYQEVAALFHIGT 300

Query: 301 TKSHPPIPEHLSAEAKDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVES 360
           TKSHPPIPEHLSAE+KDFLLKCLQ+EP+ R +AS+LLQHPFV+ E+Q+    +R+S + +
Sbjct: 301 TKSHPPIPEHLSAESKDFLLKCLQKEPHLRHSASNLLQHPFVTAEHQEARPFLRSSFMGN 360

Query: 361 GKQMADSGLNSKDLTNSTI--QRSTCVGLKDICEMGSLRCSSVYSGN-YGSRSNWGSSNF 420
            + MA   +   D+  S I   R++C GLKD+C + ++RCS+VY  N  G  S W   N 
Sbjct: 361 PENMAAQRM---DVRTSIIPDMRASCNGLKDVCGVSAVRCSTVYPENSLGKESLWKLGNS 420

Query: 421 EDDMCQIDDKDLLVSSSIKYRSMISSNDLNKSFNPMCEPTDDWACKLDESSELGGNLMDL 480
           +DDMCQ+D+ D +  +S+K  S + S    KSFNPMCEP +DW CK DES EL  +  +L
Sbjct: 421 DDDMCQMDNDDFMFGASVKCSSDLHSPANYKSFNPMCEPDNDWPCKFDESPELTKSQANL 480

Query: 481 SGIQTSQETT-------CKETGFSFPCGQSAAEDDEEVTESKIRAFLDEKAVELKKLQTP 540
              Q + + T        ++  F+FP GQSAAEDD+E+TESKIRAFLDEKA++LKKLQTP
Sbjct: 481 HYDQATIKPTNNPIMSYKEDLAFTFPSGQSAAEDDDELTESKIRAFLDEKAMDLKKLQTP 540

Query: 541 LYEEFRSTINAANALGTVGTEQGKNVSNFLKLPPKCGSPSKLRGKRLSSIDAGNCS---- 600
           LYE F +++N ++    VGT   +NV + + LPPK  SP ++  +RLS+   G C+    
Sbjct: 541 LYEGFYNSLNVSSTPSPVGTGNKENVPSNINLPPKSRSPKRMLSRRLSTAIEGACAPSPV 600

Query: 601 VRQSRIKQASILQDRALQEIQSPQLGEWNKLLHTQSDSCTPSFSERQRKWKEELEEELKR 634
               RI     L   A+QE Q P+  EW  LL +Q ++   SFSERQR+WKEEL+EEL+R
Sbjct: 601 THSKRISNIGGLNGEAIQEAQLPRHNEWKDLLGSQREAVNSSFSERQRRWKEELDEELQR 659

BLAST of Sgr016133 vs. ExPASy Swiss-Prot
Match: O22042 (Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana OX=3702 GN=ANP3 PE=1 SV=1)

HSP 1 Score: 708.4 bits (1827), Expect = 8.8e-203
Identity = 393/648 (60.65%), Postives = 463/648 (71.45%), Query Frame = 0

Query: 1   MQDFFGSVRRSIVF----LAPDGDDGGRFGGLVEKIGSSIRKSRNGLFSKHSVRALPPVA 60
           MQD  GSVRRS+VF       DG  GG   G V KI SSIR SR GLFSK     LP   
Sbjct: 1   MQDILGSVRRSLVFRSSLAGDDGTSGGGLSGFVGKINSSIRSSRIGLFSK-PPPGLPAPR 60

Query: 61  KEDSPPIWWRKGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRELE 120
           KE++P I WRKGELIGCGAFGRVYMGMNLDSGELLA+KQV IA +SAS+EK Q HIRELE
Sbjct: 61  KEEAPSIRWRKGELIGCGAFGRVYMGMNLDSGELLAIKQVLIAPSSASKEKTQGHIRELE 120

Query: 121 EEVKLLKNLSHPNIVRYLGTAREEESLNILLEFVPGGSISSLLGKFGSFPESVIRTYTKQ 180
           EEV+LLKNLSHPNIVRYLGT RE +SLNIL+EFVPGGSISSLL KFGSFPE VI  YTKQ
Sbjct: 121 EEVQLLKNLSHPNIVRYLGTVRESDSLNILMEFVPGGSISSLLEKFGSFPEPVIIMYTKQ 180

Query: 181 LLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYW 240
           LLLGLEYLH NGIMHRDIKGANILVDNKGCI+LADFGASKKVVELAT+NGAKSMKGTPYW
Sbjct: 181 LLLGLEYLHNNGIMHRDIKGANILVDNKGCIRLADFGASKKVVELATVNGAKSMKGTPYW 240

Query: 241 MAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHL 300
           MAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWS+QYQ+ AA+ HIG TK+HPPIPE L
Sbjct: 241 MAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSEQYQQFAAVLHIGRTKAHPPIPEDL 300

Query: 301 SAEAKDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVESGKQMADSGLNS 360
           S EAKDFL+KCL +EP+ R +A++LLQHPFV+ + Q+P+ A R S  E G  +   G+N 
Sbjct: 301 SPEAKDFLMKCLHKEPSLRLSATELLQHPFVTGKRQEPYPAYRNSLTECGNPITTQGMNV 360

Query: 361 KDLTNSTIQRSTCVGLKDICEMGSLRCSSVY---SGNYGSRSNWGSSNFEDDMCQIDDKD 420
           +   NS I+RSTC GLKD+CE+GSLR S +Y   S N G     G S   DD+CQ D  D
Sbjct: 361 RSSINSLIRRSTCSGLKDVCELGSLRSSIIYPQKSNNSGFGWRDGDS---DDLCQTDMDD 420

Query: 421 LLVSSSIKYRSMISSNDLNKSFNPMCEPTDDWACKLDESSELGGNLMDLSGIQTSQETT- 480
           L    S++   +  S DLNKSFNPMC+ TD+W+CK DES ++  +  +L   Q SQ  T 
Sbjct: 421 LCNIESVRNNVLSQSTDLNKSFNPMCDSTDNWSCKFDESPKVMKSKSNLLSYQASQLQTG 480

Query: 481 --C-KETGFSFPCGQSAAEDDEEVTESKIRAFLDEKAVELKKLQTPLYEEFRSTINAANA 540
             C +ET  +F  G S AEDD + TE KI++FLDEKA +LK+LQTPL EEF + +N    
Sbjct: 481 VPCDEETSLTFAGGSSVAEDDYKGTELKIKSFLDEKAQDLKRLQTPLLEEFHNAMNPGIP 540

Query: 541 LGTVGTEQGKNVSNFLKLPPKCGSPSKLRGKRLSSIDAGNCSVRQSRIKQASILQDRALQ 600
            G +G     N+ N   LP    +P +L  +RLS+I     S  +S   + ++   R +Q
Sbjct: 541 QGALG---DTNIYNLPNLPSISKTPKRLPSRRLSAISDAMPSPLKS--SKRTLNTSRVMQ 600

Query: 601 EIQSP-QLGEWNKLLHTQSDSCTPSFSERQRKWKEELEEELKRKRAEL 637
               P Q+ E  K    +  + +  FSE +RKW+EEL EEL+R R  L
Sbjct: 601 SGTEPTQVNESTK----KGVNNSRCFSEIRRKWEEELYEELERHRENL 635

BLAST of Sgr016133 vs. ExPASy Swiss-Prot
Match: O22040 (Mitogen-activated protein kinase kinase kinase ANP1 OS=Arabidopsis thaliana OX=3702 GN=ANP1 PE=1 SV=2)

HSP 1 Score: 617.1 bits (1590), Expect = 2.7e-175
Identity = 369/671 (54.99%), Postives = 446/671 (66.47%), Query Frame = 0

Query: 1   MQDFFGSVRRSIVFLAPDGDDGGRF-----GGLVEKIGSSIRKSRNGLFSKHSVRALPPV 60
           MQDFFGSVRRS+VF     DD         G L +KI S IRKS+  +F K S    PP 
Sbjct: 1   MQDFFGSVRRSLVFRPSSDDDNQENQPPFPGVLADKITSCIRKSK--IFIKPSFSPPPPA 60

Query: 61  AKED-SPPIWWRKGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRE 120
              D +PPI WRKG+LIG GAFG VYMGMNLDSGELLAVKQV IAAN AS+EK QAHI+E
Sbjct: 61  NTVDMAPPISWRKGQLIGRGAFGTVYMGMNLDSGELLAVKQVLIAANFASKEKTQAHIQE 120

Query: 121 LEEEVKLLKNLSHPNIVRYLGTAREEESLNILLEFVPGGSISSLLGKFGSFPESVIRTYT 180
           LEEEVKLLKNLSHPNIVRYLGT RE+++LNILLEFVPGGSISSLL KFG FPESV+RTYT
Sbjct: 121 LEEEVKLLKNLSHPNIVRYLGTVREDDTLNILLEFVPGGSISSLLEKFGPFPESVVRTYT 180

Query: 181 KQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTP 240
           +QLLLGLEYLH + IMHRDIKGANILVDNKGCIKLADFGASK+V ELAT+ GAKSMKGTP
Sbjct: 181 RQLLLGLEYLHNHAIMHRDIKGANILVDNKGCIKLADFGASKQVAELATMTGAKSMKGTP 240

Query: 241 YWMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPE 300
           YWMAPEVILQTGHSFSADIWSVGCTVIEM TGK PWSQQY+EVAA+F IG TKSHPPIP+
Sbjct: 241 YWMAPEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEVAAIFFIGTTKSHPPIPD 300

Query: 301 HLSAEAKDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVESGKQMADSGL 360
            LS++AKDFLLKCLQE PN RPTAS+LL+HPFV  ++++  +    S + +        +
Sbjct: 301 TLSSDAKDFLLKCLQEVPNLRPTASELLKHPFVMGKHKESASTDLGSVLNNLSTPLPLQI 360

Query: 361 NSKDLTNSTIQRSTCVGLKDICEMGSLRCS------SVYSGNYGSRSNWGSSNFEDDMCQ 420
           N+   T ST   STC  + D+C  GSL  S      S+ + N   +++ G    EDDMC 
Sbjct: 361 NN---TKST-PDSTCDDVGDMCNFGSLNYSLVDPVKSIQNKNLWQQNDNGGD--EDDMCL 420

Query: 421 IDDKDLLVSSSIKYRSMISSNDLNKSFNPMCEPTDDWACKLDESSELGGNLMDLSGIQTS 480
           IDD++ L        ++     L KS + + + +     K DES   G            
Sbjct: 421 IDDENFLTFDGEMSSTLEKDCHLKKSCDDISDMSIALKSKFDESPGNGE----------- 480

Query: 481 QETTCKETGFSFPCGQ-SAAEDDEEVTESKIRAFLDEKAVELKKLQTPLYEEFRSTI--- 540
                KE+  S  C Q S +EDD+E+TESKI+AFLDEKA +LKKLQTPLYEEF +++   
Sbjct: 481 -----KESTMSMECDQPSYSEDDDELTESKIKAFLDEKAADLKKLQTPLYEEFYNSLITF 540

Query: 541 -NAANALGTVGTEQGKNVSNFLKLPPKCGSPSK--LRGKRLSSIDAGNCSVRQ----SRI 600
             +        +++      FLKLPPK  SPS+  L G    + DA +CS       SR 
Sbjct: 541 SPSCMESNLSNSKREDTARGFLKLPPKSRSPSRGPLGGSPSRATDATSCSKSPGSGGSRE 600

Query: 601 KQASILQDRALQEIQSPQLGEWNKLL--HTQSDSCTPSFSERQRKWKEELEEELKRKRAE 647
              +   D A Q+  S ++ +W  L+    Q  S   + SE ++KWKEEL++EL+RKR E
Sbjct: 601 LNINNGGDEASQDGVSARVTDWRGLVVDTKQELSQCVALSEIEKKWKEELDQELERKRQE 647

BLAST of Sgr016133 vs. ExPASy Swiss-Prot
Match: Q9FZ36 (Mitogen-activated protein kinase kinase kinase 2 OS=Arabidopsis thaliana OX=3702 GN=ANP2 PE=2 SV=1)

HSP 1 Score: 605.1 bits (1559), Expect = 1.0e-171
Identity = 362/669 (54.11%), Postives = 448/669 (66.97%), Query Frame = 0

Query: 1   MQDFFGSVRRSIVFLAPDGDDGGR-----FGGLV-EKIGSSIRKSRNGLFSKHSVRALPP 60
           MQD FGSVRRS+VF +   D+        F  L+ +KI S IRKS   +F+K      PP
Sbjct: 1   MQDLFGSVRRSLVFRSTTDDENQENHPPPFPSLLADKITSCIRKSM--VFAKSQ---SPP 60

Query: 61  --VAKEDSPPIWWRKGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHI 120
                +  PPI WRKG+LIG GAFG VYMGMNLDSGELLAVKQV I +N AS+EK QAHI
Sbjct: 61  NNSTVQIKPPIRWRKGQLIGRGAFGTVYMGMNLDSGELLAVKQVLITSNCASKEKTQAHI 120

Query: 121 RELEEEVKLLKNLSHPNIVRYLGTAREEESLNILLEFVPGGSISSLLGKFGSFPESVIRT 180
           +ELEEEVKLLKNLSHPNIVRYLGT RE+E+LNILLEFVPGGSISSLL KFG+FPESV+RT
Sbjct: 121 QELEEEVKLLKNLSHPNIVRYLGTVREDETLNILLEFVPGGSISSLLEKFGAFPESVVRT 180

Query: 181 YTKQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKG 240
           YT QLLLGLEYLH + IMHRDIKGANILVDN+GCIKLADFGASK+V ELATI+GAKSMKG
Sbjct: 181 YTNQLLLGLEYLHNHAIMHRDIKGANILVDNQGCIKLADFGASKQVAELATISGAKSMKG 240

Query: 241 TPYWMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPI 300
           TPYWMAPEVILQTGHSFSADIWSVGCTVIEM TGK PWSQQY+E+AA+FHIG TKSHPPI
Sbjct: 241 TPYWMAPEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEIAAIFHIGTTKSHPPI 300

Query: 301 PEHLSAEAKDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVESGKQMADS 360
           P+++S++A DFLLKCLQ+EPN RPTAS+LL+HPFV+ + ++  +    S +++      S
Sbjct: 301 PDNISSDANDFLLKCLQQEPNLRPTASELLKHPFVTGKQKESASKDLTSFMDNSCSPLPS 360

Query: 361 GLNSKDLTNST-IQRSTCVGLKDICEMGSLRCS------SVYSGNYGSRSNWGSSNFED- 420
                +LTN T  Q ST   + DIC +GSL C+      S+ + +   +SN G  + +D 
Sbjct: 361 -----ELTNITSYQTSTSDDVGDICNLGSLTCTLAFPEKSIQNNSLCLKSNNGYDDDDDN 420

Query: 421 DMCQIDDKDLLVSSSIKYRSMISSNDLNKSFNPMCEPTDDWACKLDESSELGGNLMDLSG 480
           DMC IDD++ L  +     S+ ++ D  KS + M E +D   CK DE+S  G        
Sbjct: 421 DMCLIDDENFLTYNGETGPSLDNNTDAKKSCDTMSEISDILKCKFDENSGNG-------- 480

Query: 481 IQTSQETTCKETGFSFPCGQ-SAAEDDEEVTESKIRAFLDEKAVELKKLQTPLYEEFRST 540
                ET   ET  S      S +ED+ E+TESKI+AFLD+KA ELKKLQTPLYEEF + 
Sbjct: 481 -----ET---ETKVSMEVDHPSYSEDENELTESKIKAFLDDKAAELKKLQTPLYEEFYNG 540

Query: 541 INAANAL----GTVGTEQGKNVSNFLKLPPKCGSPSKLRGKRLSS--IDAGNCSVRQSRI 600
           +   + +         ++ +    FLKLPPK  SPS+    R  S   DA  CS      
Sbjct: 541 MITCSPICMESNINNNKREEAPRGFLKLPPKSRSPSQGHIGRSPSRATDAACCSKSPESG 600

Query: 601 KQASILQDRALQEIQSPQLGEWNKLLHTQSDSCTPSFSERQRKWKEELEEELKRKRAELS 647
             +            +P+    +     +S+S + + SE +RKWKEEL++EL+RKR E++
Sbjct: 601 NSSG-----------APKNSNASAGAEQESNSQSVALSEIERKWKEELDQELERKRREIT 632

BLAST of Sgr016133 vs. ExPASy Swiss-Prot
Match: Q54R82 (Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum OX=44689 GN=mkkA PE=1 SV=2)

HSP 1 Score: 305.8 bits (782), Expect = 1.3e-81
Identity = 142/265 (53.58%), Postives = 196/265 (73.96%), Query Frame = 0

Query: 63  IWWRKGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRELEEEVKLL 122
           I W+KG+++G G +G VY+G+N D+GEL AVKQ+ I  +  S  K +  I    +E++++
Sbjct: 168 IKWQKGQILGRGGYGSVYLGLNKDTGELFAVKQLEI-VDINSDPKLKNMILSFSKEIEVM 227

Query: 123 KNLSHPNIVRYLGTAREEESLNILLEFVPGGSISSLLGKFGSFPESVIRTYTKQLLLGLE 182
           ++L H NIVRYLGT+ ++  L++ LE++PGGSISSLLGKFG+F E+VI+ YTKQ+L GL 
Sbjct: 228 RSLRHDNIVRYLGTSLDQSFLSVFLEYIPGGSISSLLGKFGAFSENVIKVYTKQILQGLS 287

Query: 183 YLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPEVI 242
           +LH N I+HRDIKGANIL+D KG +KL+DFG SK    +  ++  KSM+GTPYWMAPEVI
Sbjct: 288 FLHANSIIHRDIKGANILIDTKGIVKLSDFGCSKSFSGI--VSQFKSMQGTPYWMAPEVI 347

Query: 243 LQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEAKD 302
            QTGH  S+DIWS+GC ++EMAT +PPWS   +  A ++HI ++ S P IP H+S EA D
Sbjct: 348 KQTGHGRSSDIWSLGCVIVEMATAQPPWSNITELAAVMYHIASSNSIPNIPSHMSQEAFD 407

Query: 303 FLLKCLQEEPNSRPTASDLLQHPFV 328
           FL  C + +P  RP A+ LL+HPF+
Sbjct: 408 FLNLCFKRDPKERPDANQLLKHPFI 429

BLAST of Sgr016133 vs. ExPASy TrEMBL
Match: A0A6J1CE75 (mitogen-activated protein kinase kinase kinase NPK1 OS=Momordica charantia OX=3673 GN=LOC111009894 PE=4 SV=1)

HSP 1 Score: 1192.6 bits (3084), Expect = 0.0e+00
Identity = 596/634 (94.01%), Postives = 611/634 (96.37%), Query Frame = 0

Query: 1   MQDFFGSVRRSIVFLAPDGDDGGRFGGLVEKIGSSIRKSRNGLFSKHSVRALPPVAKEDS 60
           MQDFFGSVRRS+VFL PDGDDGGRFGGLVEKIGSSIRKSRNGLFSKHSVRALPPV KED+
Sbjct: 1   MQDFFGSVRRSLVFLTPDGDDGGRFGGLVEKIGSSIRKSRNGLFSKHSVRALPPVMKEDA 60

Query: 61  PPIWWRKGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRELEEEVK 120
           PPIWWR+GELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRELEEEVK
Sbjct: 61  PPIWWRRGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRELEEEVK 120

Query: 121 LLKNLSHPNIVRYLGTAREEESLNILLEFVPGGSISSLLGKFGSFPESVIRTYTKQLLLG 180
           LLKNLSHPNIVRYLGT R E+SLNILLEFVPGGSISSLLGKFGSFPESVIRTYTKQLLLG
Sbjct: 121 LLKNLSHPNIVRYLGTTRGEDSLNILLEFVPGGSISSLLGKFGSFPESVIRTYTKQLLLG 180

Query: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240
           LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE
Sbjct: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240

Query: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300
           VILQTGHSF ADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA
Sbjct: 241 VILQTGHSFPADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300

Query: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVESGKQMADSGLNSKDLT 360
           KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVESGKQM  SGLNS DL 
Sbjct: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVESGKQMVGSGLNSNDLA 360

Query: 361 NSTIQRSTCVGLKDICEMGSLRCSSVYSGNYGSRSNWGSSNFEDDMCQIDDKDLLVSSSI 420
           NSTIQRSTCVGLKDICEMGSLRCSSVYSGNY S+SNWGSSNFE+DMCQIDDKDLLVS SI
Sbjct: 361 NSTIQRSTCVGLKDICEMGSLRCSSVYSGNYTSQSNWGSSNFEEDMCQIDDKDLLVSPSI 420

Query: 421 KYRSMISSNDLNKSFNPMCEPT-DDWACKLDESSELGGNLMDLSGIQTSQETTCKETGFS 480
           KY+S+ISS+DLNKSFNPMCEPT DDWACKLD SSELGGNLMDLSGIQTSQETTCKE  F+
Sbjct: 421 KYKSIISSSDLNKSFNPMCEPTDDDWACKLDGSSELGGNLMDLSGIQTSQETTCKENDFT 480

Query: 481 FPCGQSAAEDDEEVTESKIRAFLDEKAVELKKLQTPLYEEFRSTINAANALGTVGTEQGK 540
           FPCGQSA EDDEEVTESKIRAFLDEKA+ELKK+QTPLYEEFRSTINAANALG VGTE  K
Sbjct: 481 FPCGQSAVEDDEEVTESKIRAFLDEKAIELKKMQTPLYEEFRSTINAANALGAVGTENSK 540

Query: 541 NVSNFLKLPPKCGSPSKLRGKRLSSIDAGNCSVRQSRIKQASILQDRALQEIQSPQLGEW 600
           +VSNFLKLPPKCGSPSKLRGKRLSSIDA NCS RQSRIKQASIL+DRALQEIQSPQLGEW
Sbjct: 541 SVSNFLKLPPKCGSPSKLRGKRLSSIDASNCSSRQSRIKQASILEDRALQEIQSPQLGEW 600

Query: 601 NKLLHTQSDSCTPSFSERQRKWKEELEEELKRKR 634
           NKLLHTQ +S TPSFSERQRKWKEELEEELKRKR
Sbjct: 601 NKLLHTQPESSTPSFSERQRKWKEELEEELKRKR 634

BLAST of Sgr016133 vs. ExPASy TrEMBL
Match: A0A6J1KXU2 (mitogen-activated protein kinase kinase kinase NPK1 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111499179 PE=4 SV=1)

HSP 1 Score: 1181.8 bits (3056), Expect = 0.0e+00
Identity = 584/633 (92.26%), Postives = 611/633 (96.52%), Query Frame = 0

Query: 1   MQDFFGSVRRSIVFLAPDGDDGGRFGGLVEKIGSSIRKSRNGLFSKHSVRALPPVAKEDS 60
           MQDFFGSVRRS+VFLAPDGDDGG+FGGLVE+IGSSIRKSRNGLFSKHSVRALPPVAKED+
Sbjct: 1   MQDFFGSVRRSLVFLAPDGDDGGKFGGLVERIGSSIRKSRNGLFSKHSVRALPPVAKEDA 60

Query: 61  PPIWWRKGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRELEEEVK 120
           PPIWWRKGELIGCGAFGRVYMGMNLDSGEL+A+KQVSIAANSASREKAQAHIRELEEEVK
Sbjct: 61  PPIWWRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHIRELEEEVK 120

Query: 121 LLKNLSHPNIVRYLGTAREEESLNILLEFVPGGSISSLLGKFGSFPESVIRTYTKQLLLG 180
           LLKNLSHPNIVRYLGTAREE SLNILLEFVPGGSISSLLGKFGSFPESV++TYTKQLLLG
Sbjct: 121 LLKNLSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKTYTKQLLLG 180

Query: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240
           LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE
Sbjct: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240

Query: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300
           VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA
Sbjct: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300

Query: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVESGKQMADSGLNSKDLT 360
           KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQ+PHAAVR SS+ESGKQMADS LNSKD+T
Sbjct: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQEPHAAVRVSSLESGKQMADSRLNSKDIT 360

Query: 361 NSTIQRSTCVGLKDICEMGSLRCSSVYSGNYGSRSNWGSSNFEDDMCQIDDKDLLVSSSI 420
            S IQRSTC GLKDICEMGSLRCSSV+SGNY  RSNWGSSNFEDDMCQIDDK+L  SSSI
Sbjct: 361 KSIIQRSTCEGLKDICEMGSLRCSSVFSGNYEVRSNWGSSNFEDDMCQIDDKNLFASSSI 420

Query: 421 KYRSMISSNDLNKSFNPMCEPTDDWACKLDESSELGGNLMDLSGIQTSQETTCKETGFSF 480
           KY+S+ISSNDLNKSFNPMCEPTDDWACKLDESSEL GNLM+LSGI+T QE+T K   F+F
Sbjct: 421 KYKSIISSNDLNKSFNPMCEPTDDWACKLDESSELRGNLMELSGIRTGQESTSKGNDFTF 480

Query: 481 PCGQSAAEDDEEVTESKIRAFLDEKAVELKKLQTPLYEEFRSTINAANALGTVGTEQGKN 540
           PCGQS AEDDEEVT+SKI+AFLDEKA+ELKKLQTPLYEEFRST+NAANALG VGTE  K+
Sbjct: 481 PCGQSTAEDDEEVTDSKIQAFLDEKALELKKLQTPLYEEFRSTVNAANALGAVGTENSKS 540

Query: 541 VSNFLKLPPKCGSPSKLRGKRLSSIDAGNCSVRQSRIKQASILQDRALQEIQSPQLGEWN 600
           VSNFLKLPPKCGSPSKLRGKRLSSIDAGNCS RQSRIKQASILQDRALQEIQSPQ+GEWN
Sbjct: 541 VSNFLKLPPKCGSPSKLRGKRLSSIDAGNCSSRQSRIKQASILQDRALQEIQSPQIGEWN 600

Query: 601 KLLHTQSDSCTPSFSERQRKWKEELEEELKRKR 634
           KLLHTQ +SCTP+FSERQRKWKEELEEELKRKR
Sbjct: 601 KLLHTQPESCTPTFSERQRKWKEELEEELKRKR 633

BLAST of Sgr016133 vs. ExPASy TrEMBL
Match: A0A6J1L4Z4 (mitogen-activated protein kinase kinase kinase NPK1 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111499179 PE=4 SV=1)

HSP 1 Score: 1177.2 bits (3044), Expect = 0.0e+00
Identity = 584/634 (92.11%), Postives = 611/634 (96.37%), Query Frame = 0

Query: 1   MQDFFGSVRRSIVFLAPDGDDGGRFGGLVEKIGSSIRKSRNGLFSKHSVRALPPVAKEDS 60
           MQDFFGSVRRS+VFLAPDGDDGG+FGGLVE+IGSSIRKSRNGLFSKHSVRALPPVAKED+
Sbjct: 1   MQDFFGSVRRSLVFLAPDGDDGGKFGGLVERIGSSIRKSRNGLFSKHSVRALPPVAKEDA 60

Query: 61  PPIWWRKGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRELEEEVK 120
           PPIWWRKGELIGCGAFGRVYMGMNLDSGEL+A+KQVSIAANSASREKAQAHIRELEEEVK
Sbjct: 61  PPIWWRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHIRELEEEVK 120

Query: 121 LLKNLSHPNIVRYLGTAREEESLNILLEFVPGGSISSLLGKFGSFPESVIRTYTKQLLLG 180
           LLKNLSHPNIVRYLGTAREE SLNILLEFVPGGSISSLLGKFGSFPESV++TYTKQLLLG
Sbjct: 121 LLKNLSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKTYTKQLLLG 180

Query: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240
           LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE
Sbjct: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240

Query: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300
           VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA
Sbjct: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300

Query: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVESGKQMADSGLNSKDLT 360
           KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQ+PHAAVR SS+ESGKQMADS LNSKD+T
Sbjct: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQEPHAAVRVSSLESGKQMADSRLNSKDIT 360

Query: 361 NSTIQRSTCVGLKDICEMGSLRCSSVYSGNYGSRSNWGSSNFEDDMCQIDDKDLLVSSSI 420
            S IQRSTC GLKDICEMGSLRCSSV+SGNY  RSNWGSSNFEDDMCQIDDK+L  SSSI
Sbjct: 361 KSIIQRSTCEGLKDICEMGSLRCSSVFSGNYEVRSNWGSSNFEDDMCQIDDKNLFASSSI 420

Query: 421 KYRSMISSNDLNKSFNPMCEPTDDWACKLDESSELGGNLMDLSGIQTSQETTCKETGFSF 480
           KY+S+ISSNDLNKSFNPMCEPTDDWACKLDESSEL GNLM+LSGI+T QE+T K   F+F
Sbjct: 421 KYKSIISSNDLNKSFNPMCEPTDDWACKLDESSELRGNLMELSGIRTGQESTSKGNDFTF 480

Query: 481 PCGQSAAEDDEEVTESKIRAFLDEKAVELKKLQTPLYEEFRSTINAANALGTVGTEQGKN 540
           PCGQS AEDDEEVT+SKI+AFLDEKA+ELKKLQTPLYEEFRST+NAANALG VGTE  K+
Sbjct: 481 PCGQSTAEDDEEVTDSKIQAFLDEKALELKKLQTPLYEEFRSTVNAANALGAVGTENSKS 540

Query: 541 VSNFLKLPPKCGSPSKLRGKRLSSIDAGNCSVRQSRIKQASILQDRALQEIQSPQLGEWN 600
           VSNFLKLPPKCGSPSKLRGKRLSSIDAGNCS RQSRIKQASILQDRALQEIQSPQ+GEWN
Sbjct: 541 VSNFLKLPPKCGSPSKLRGKRLSSIDAGNCSSRQSRIKQASILQDRALQEIQSPQIGEWN 600

Query: 601 KLLHTQSDSCTPS-FSERQRKWKEELEEELKRKR 634
           KLLHTQ +SCTP+ FSERQRKWKEELEEELKRKR
Sbjct: 601 KLLHTQPESCTPTFFSERQRKWKEELEEELKRKR 634

BLAST of Sgr016133 vs. ExPASy TrEMBL
Match: A0A6J1H661 (mitogen-activated protein kinase kinase kinase NPK1 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111459961 PE=4 SV=1)

HSP 1 Score: 1173.7 bits (3035), Expect = 0.0e+00
Identity = 581/633 (91.79%), Postives = 607/633 (95.89%), Query Frame = 0

Query: 1   MQDFFGSVRRSIVFLAPDGDDGGRFGGLVEKIGSSIRKSRNGLFSKHSVRALPPVAKEDS 60
           MQDFFGSVRRS+VFLAPDGDDGG+FGGLVE+IGSSIRKSRNGLFSKHSVRALPPVAKED+
Sbjct: 1   MQDFFGSVRRSLVFLAPDGDDGGKFGGLVERIGSSIRKSRNGLFSKHSVRALPPVAKEDA 60

Query: 61  PPIWWRKGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRELEEEVK 120
           PPIWWRKGELIGCGAFGRVYMGMNLDSGEL+A+KQVSIAANSASREKAQAHIRELEEEVK
Sbjct: 61  PPIWWRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHIRELEEEVK 120

Query: 121 LLKNLSHPNIVRYLGTAREEESLNILLEFVPGGSISSLLGKFGSFPESVIRTYTKQLLLG 180
           LLKNLSHPNIVRYLGTAREE SLNILLEFVPGGSISSLLGKFGSFPESV++TYTKQLLLG
Sbjct: 121 LLKNLSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKTYTKQLLLG 180

Query: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240
           LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE
Sbjct: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240

Query: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300
           VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA
Sbjct: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300

Query: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVESGKQMADSGLNSKDLT 360
           KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQ+PHAAVRASS+ESGKQMA + LNS D+T
Sbjct: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQEPHAAVRASSLESGKQMAGARLNSNDIT 360

Query: 361 NSTIQRSTCVGLKDICEMGSLRCSSVYSGNYGSRSNWGSSNFEDDMCQIDDKDLLVSSSI 420
            S IQRSTC GLKDICEMGSLRCSSV+SGNY  RSNWGSSN EDDMCQIDDKDL  SSSI
Sbjct: 361 KSIIQRSTCEGLKDICEMGSLRCSSVFSGNYEVRSNWGSSNSEDDMCQIDDKDLFASSSI 420

Query: 421 KYRSMISSNDLNKSFNPMCEPTDDWACKLDESSELGGNLMDLSGIQTSQETTCKETGFSF 480
           KY+S+ISSNDLNKSFNPMCEPTDDWACKLDESSEL GNLM+LSGIQT QE+T K   F+F
Sbjct: 421 KYKSIISSNDLNKSFNPMCEPTDDWACKLDESSELRGNLMELSGIQTGQESTSKGNDFTF 480

Query: 481 PCGQSAAEDDEEVTESKIRAFLDEKAVELKKLQTPLYEEFRSTINAANALGTVGTEQGKN 540
           PCGQS AEDDEEVT+SKI+AFLDEKA+ELKKLQTPLYEEFRST+NAANALG  GTE  K+
Sbjct: 481 PCGQSTAEDDEEVTDSKIQAFLDEKALELKKLQTPLYEEFRSTVNAANALGAGGTENSKS 540

Query: 541 VSNFLKLPPKCGSPSKLRGKRLSSIDAGNCSVRQSRIKQASILQDRALQEIQSPQLGEWN 600
           VSNFLKLPPKCGSPSKLRGKRLSSID GNCS RQSR+KQASILQDRALQEIQSPQ+GEWN
Sbjct: 541 VSNFLKLPPKCGSPSKLRGKRLSSIDDGNCSSRQSRVKQASILQDRALQEIQSPQIGEWN 600

Query: 601 KLLHTQSDSCTPSFSERQRKWKEELEEELKRKR 634
           KLLHTQ +SCTPSFSERQRKWKEELEEELKRKR
Sbjct: 601 KLLHTQPESCTPSFSERQRKWKEELEEELKRKR 633

BLAST of Sgr016133 vs. ExPASy TrEMBL
Match: A0A6J1H434 (mitogen-activated protein kinase kinase kinase NPK1 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111459961 PE=4 SV=1)

HSP 1 Score: 1167.1 bits (3018), Expect = 0.0e+00
Identity = 580/634 (91.48%), Postives = 606/634 (95.58%), Query Frame = 0

Query: 1   MQDFFGSVRRSIVFLAPDGDDGGRFGGLVEKIGSSIRKSRNGLFSKHSVRALPPVAKEDS 60
           MQDFFGSVRRS+VFLAPDGDDGG+FGGLVE+IGSSIRKSRNGLFSKHSVRALPPVAKED+
Sbjct: 1   MQDFFGSVRRSLVFLAPDGDDGGKFGGLVERIGSSIRKSRNGLFSKHSVRALPPVAKEDA 60

Query: 61  PPIWWRKGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRELEEEVK 120
           PPIWWRKGELIGCGAFGRVYMGMNLDSGEL+A+KQVSIAANSASREKAQAHIRELEEEVK
Sbjct: 61  PPIWWRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHIRELEEEVK 120

Query: 121 LLKNLSHPNIVRYLGTAREEESLNILLEFVPGGSISSLLGKFGSFPESVIRTYTKQLLLG 180
           LLKNLSHPNIVRYLGTAREE SLNILLEFVPGGSISSLLGKFGSFPESV++TYTKQLLLG
Sbjct: 121 LLKNLSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKTYTKQLLLG 180

Query: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240
           LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE
Sbjct: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240

Query: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300
           VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA
Sbjct: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300

Query: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVESGKQMADSGLNSKDLT 360
           KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQ+PHAAVRASS+ESGKQMA + LNS D+T
Sbjct: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQEPHAAVRASSLESGKQMAGARLNSNDIT 360

Query: 361 NSTIQRSTCVGLKDICEMGSLRCSSVYSGNYGSRSNWGSSNFEDDMCQIDDKDLLVSSSI 420
            S IQRSTC GLKDICEMGSLRCSSV+SGNY  RSNWGSSN EDDMCQIDDKDL  SSSI
Sbjct: 361 KSIIQRSTCEGLKDICEMGSLRCSSVFSGNYEVRSNWGSSNSEDDMCQIDDKDLFASSSI 420

Query: 421 KYRSMISSNDLNKSFNPMCEPTDDWACKLDESSELGGNLMDLSGIQTSQETTCKETGFSF 480
           KY+S+ISSNDLNKSFNPMCEPTDDWACKLDESSEL GNLM+LSGIQT QE+T K   F+F
Sbjct: 421 KYKSIISSNDLNKSFNPMCEPTDDWACKLDESSELRGNLMELSGIQTGQESTSKGNDFTF 480

Query: 481 PCGQSAAEDDEEVTESKIRAFLDEKAVELKKLQTPLYEEFRSTINAANALGTVGTEQGKN 540
           PCGQS AEDDEEVT+SKI+AFLDEKA+ELKKLQTPLYEEFRST+NAANALG  GTE  K+
Sbjct: 481 PCGQSTAEDDEEVTDSKIQAFLDEKALELKKLQTPLYEEFRSTVNAANALGAGGTENSKS 540

Query: 541 VSNFLKLPPKCGSPSKLRGKRLSSIDAGNCSVRQSRIKQASILQDRALQEIQSPQLGEWN 600
           VSNFLKLPPKCGSPSKLRGKRLSSID GNCS RQSR+KQASILQDRALQEIQSPQ+GEWN
Sbjct: 541 VSNFLKLPPKCGSPSKLRGKRLSSIDDGNCSSRQSRVKQASILQDRALQEIQSPQIGEWN 600

Query: 601 KLLHTQSDSCTPS-FSERQRKWKEELEEELKRKR 634
           KLLHTQ +SCTP  FSERQRKWKEELEEELKRKR
Sbjct: 601 KLLHTQPESCTPRFFSERQRKWKEELEEELKRKR 634

BLAST of Sgr016133 vs. TAIR 10
Match: AT3G06030.1 (NPK1-related protein kinase 3 )

HSP 1 Score: 708.4 bits (1827), Expect = 6.2e-204
Identity = 393/648 (60.65%), Postives = 463/648 (71.45%), Query Frame = 0

Query: 1   MQDFFGSVRRSIVF----LAPDGDDGGRFGGLVEKIGSSIRKSRNGLFSKHSVRALPPVA 60
           MQD  GSVRRS+VF       DG  GG   G V KI SSIR SR GLFSK     LP   
Sbjct: 1   MQDILGSVRRSLVFRSSLAGDDGTSGGGLSGFVGKINSSIRSSRIGLFSK-PPPGLPAPR 60

Query: 61  KEDSPPIWWRKGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRELE 120
           KE++P I WRKGELIGCGAFGRVYMGMNLDSGELLA+KQV IA +SAS+EK Q HIRELE
Sbjct: 61  KEEAPSIRWRKGELIGCGAFGRVYMGMNLDSGELLAIKQVLIAPSSASKEKTQGHIRELE 120

Query: 121 EEVKLLKNLSHPNIVRYLGTAREEESLNILLEFVPGGSISSLLGKFGSFPESVIRTYTKQ 180
           EEV+LLKNLSHPNIVRYLGT RE +SLNIL+EFVPGGSISSLL KFGSFPE VI  YTKQ
Sbjct: 121 EEVQLLKNLSHPNIVRYLGTVRESDSLNILMEFVPGGSISSLLEKFGSFPEPVIIMYTKQ 180

Query: 181 LLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYW 240
           LLLGLEYLH NGIMHRDIKGANILVDNKGCI+LADFGASKKVVELAT+NGAKSMKGTPYW
Sbjct: 181 LLLGLEYLHNNGIMHRDIKGANILVDNKGCIRLADFGASKKVVELATVNGAKSMKGTPYW 240

Query: 241 MAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHL 300
           MAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWS+QYQ+ AA+ HIG TK+HPPIPE L
Sbjct: 241 MAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSEQYQQFAAVLHIGRTKAHPPIPEDL 300

Query: 301 SAEAKDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVESGKQMADSGLNS 360
           S EAKDFL+KCL +EP+ R +A++LLQHPFV+ + Q+P+ A R S  E G  +   G+N 
Sbjct: 301 SPEAKDFLMKCLHKEPSLRLSATELLQHPFVTGKRQEPYPAYRNSLTECGNPITTQGMNV 360

Query: 361 KDLTNSTIQRSTCVGLKDICEMGSLRCSSVY---SGNYGSRSNWGSSNFEDDMCQIDDKD 420
           +   NS I+RSTC GLKD+CE+GSLR S +Y   S N G     G S   DD+CQ D  D
Sbjct: 361 RSSINSLIRRSTCSGLKDVCELGSLRSSIIYPQKSNNSGFGWRDGDS---DDLCQTDMDD 420

Query: 421 LLVSSSIKYRSMISSNDLNKSFNPMCEPTDDWACKLDESSELGGNLMDLSGIQTSQETT- 480
           L    S++   +  S DLNKSFNPMC+ TD+W+CK DES ++  +  +L   Q SQ  T 
Sbjct: 421 LCNIESVRNNVLSQSTDLNKSFNPMCDSTDNWSCKFDESPKVMKSKSNLLSYQASQLQTG 480

Query: 481 --C-KETGFSFPCGQSAAEDDEEVTESKIRAFLDEKAVELKKLQTPLYEEFRSTINAANA 540
             C +ET  +F  G S AEDD + TE KI++FLDEKA +LK+LQTPL EEF + +N    
Sbjct: 481 VPCDEETSLTFAGGSSVAEDDYKGTELKIKSFLDEKAQDLKRLQTPLLEEFHNAMNPGIP 540

Query: 541 LGTVGTEQGKNVSNFLKLPPKCGSPSKLRGKRLSSIDAGNCSVRQSRIKQASILQDRALQ 600
            G +G     N+ N   LP    +P +L  +RLS+I     S  +S   + ++   R +Q
Sbjct: 541 QGALG---DTNIYNLPNLPSISKTPKRLPSRRLSAISDAMPSPLKS--SKRTLNTSRVMQ 600

Query: 601 EIQSP-QLGEWNKLLHTQSDSCTPSFSERQRKWKEELEEELKRKRAEL 637
               P Q+ E  K    +  + +  FSE +RKW+EEL EEL+R R  L
Sbjct: 601 SGTEPTQVNESTK----KGVNNSRCFSEIRRKWEEELYEELERHRENL 635

BLAST of Sgr016133 vs. TAIR 10
Match: AT1G09000.1 (NPK1-related protein kinase 1 )

HSP 1 Score: 617.1 bits (1590), Expect = 1.9e-176
Identity = 369/671 (54.99%), Postives = 446/671 (66.47%), Query Frame = 0

Query: 1   MQDFFGSVRRSIVFLAPDGDDGGRF-----GGLVEKIGSSIRKSRNGLFSKHSVRALPPV 60
           MQDFFGSVRRS+VF     DD         G L +KI S IRKS+  +F K S    PP 
Sbjct: 1   MQDFFGSVRRSLVFRPSSDDDNQENQPPFPGVLADKITSCIRKSK--IFIKPSFSPPPPA 60

Query: 61  AKED-SPPIWWRKGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRE 120
              D +PPI WRKG+LIG GAFG VYMGMNLDSGELLAVKQV IAAN AS+EK QAHI+E
Sbjct: 61  NTVDMAPPISWRKGQLIGRGAFGTVYMGMNLDSGELLAVKQVLIAANFASKEKTQAHIQE 120

Query: 121 LEEEVKLLKNLSHPNIVRYLGTAREEESLNILLEFVPGGSISSLLGKFGSFPESVIRTYT 180
           LEEEVKLLKNLSHPNIVRYLGT RE+++LNILLEFVPGGSISSLL KFG FPESV+RTYT
Sbjct: 121 LEEEVKLLKNLSHPNIVRYLGTVREDDTLNILLEFVPGGSISSLLEKFGPFPESVVRTYT 180

Query: 181 KQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTP 240
           +QLLLGLEYLH + IMHRDIKGANILVDNKGCIKLADFGASK+V ELAT+ GAKSMKGTP
Sbjct: 181 RQLLLGLEYLHNHAIMHRDIKGANILVDNKGCIKLADFGASKQVAELATMTGAKSMKGTP 240

Query: 241 YWMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPE 300
           YWMAPEVILQTGHSFSADIWSVGCTVIEM TGK PWSQQY+EVAA+F IG TKSHPPIP+
Sbjct: 241 YWMAPEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEVAAIFFIGTTKSHPPIPD 300

Query: 301 HLSAEAKDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVESGKQMADSGL 360
            LS++AKDFLLKCLQE PN RPTAS+LL+HPFV  ++++  +    S + +        +
Sbjct: 301 TLSSDAKDFLLKCLQEVPNLRPTASELLKHPFVMGKHKESASTDLGSVLNNLSTPLPLQI 360

Query: 361 NSKDLTNSTIQRSTCVGLKDICEMGSLRCS------SVYSGNYGSRSNWGSSNFEDDMCQ 420
           N+   T ST   STC  + D+C  GSL  S      S+ + N   +++ G    EDDMC 
Sbjct: 361 NN---TKST-PDSTCDDVGDMCNFGSLNYSLVDPVKSIQNKNLWQQNDNGGD--EDDMCL 420

Query: 421 IDDKDLLVSSSIKYRSMISSNDLNKSFNPMCEPTDDWACKLDESSELGGNLMDLSGIQTS 480
           IDD++ L        ++     L KS + + + +     K DES   G            
Sbjct: 421 IDDENFLTFDGEMSSTLEKDCHLKKSCDDISDMSIALKSKFDESPGNGE----------- 480

Query: 481 QETTCKETGFSFPCGQ-SAAEDDEEVTESKIRAFLDEKAVELKKLQTPLYEEFRSTI--- 540
                KE+  S  C Q S +EDD+E+TESKI+AFLDEKA +LKKLQTPLYEEF +++   
Sbjct: 481 -----KESTMSMECDQPSYSEDDDELTESKIKAFLDEKAADLKKLQTPLYEEFYNSLITF 540

Query: 541 -NAANALGTVGTEQGKNVSNFLKLPPKCGSPSK--LRGKRLSSIDAGNCSVRQ----SRI 600
             +        +++      FLKLPPK  SPS+  L G    + DA +CS       SR 
Sbjct: 541 SPSCMESNLSNSKREDTARGFLKLPPKSRSPSRGPLGGSPSRATDATSCSKSPGSGGSRE 600

Query: 601 KQASILQDRALQEIQSPQLGEWNKLL--HTQSDSCTPSFSERQRKWKEELEEELKRKRAE 647
              +   D A Q+  S ++ +W  L+    Q  S   + SE ++KWKEEL++EL+RKR E
Sbjct: 601 LNINNGGDEASQDGVSARVTDWRGLVVDTKQELSQCVALSEIEKKWKEELDQELERKRQE 647

BLAST of Sgr016133 vs. TAIR 10
Match: AT1G54960.1 (NPK1-related protein kinase 2 )

HSP 1 Score: 583.2 bits (1502), Expect = 3.0e-166
Identity = 336/601 (55.91%), Postives = 414/601 (68.89%), Query Frame = 0

Query: 61  PPIWWRKGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRELEEEVK 120
           PPI WRKG+LIG GAFG VYMGMNLDSGELLAVKQV I +N AS+EK QAHI+ELEEEVK
Sbjct: 19  PPIRWRKGQLIGRGAFGTVYMGMNLDSGELLAVKQVLITSNCASKEKTQAHIQELEEEVK 78

Query: 121 LLKNLSHPNIVRYLGTAREEESLNILLEFVPGGSISSLLGKFGSFPESVIRTYTKQLLLG 180
           LLKNLSHPNIVRYLGT RE+E+LNILLEFVPGGSISSLL KFG+FPESV+RTYT QLLLG
Sbjct: 79  LLKNLSHPNIVRYLGTVREDETLNILLEFVPGGSISSLLEKFGAFPESVVRTYTNQLLLG 138

Query: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240
           LEYLH + IMHRDIKGANILVDN+GCIKLADFGASK+V ELATI+GAKSMKGTPYWMAPE
Sbjct: 139 LEYLHNHAIMHRDIKGANILVDNQGCIKLADFGASKQVAELATISGAKSMKGTPYWMAPE 198

Query: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300
           VILQTGHSFSADIWSVGCTVIEM TGK PWSQQY+E+AA+FHIG TKSHPPIP+++S++A
Sbjct: 199 VILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEIAAIFHIGTTKSHPPIPDNISSDA 258

Query: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVESGKQMADSGLNSKDLT 360
            DFLLKCLQ+EPN RPTAS+LL+HPFV+ + ++  +    S +++      S     +LT
Sbjct: 259 NDFLLKCLQQEPNLRPTASELLKHPFVTGKQKESASKDLTSFMDNSCSPLPS-----ELT 318

Query: 361 NST-IQRSTCVGLKDICEMGSLRCS------SVYSGNYGSRSNWGSSNFED-DMCQIDDK 420
           N T  Q ST   + DIC +GSL C+      S+ + +   +SN G  + +D DMC IDD+
Sbjct: 319 NITSYQTSTSDDVGDICNLGSLTCTLAFPEKSIQNNSLCLKSNNGYDDDDDNDMCLIDDE 378

Query: 421 DLLVSSSIKYRSMISSNDLNKSFNPMCEPTDDWACKLDESSELGGNLMDLSGIQTSQETT 480
           + L  +     S+ ++ D  KS + M E +D   CK DE+S  G             ET 
Sbjct: 379 NFLTYNGETGPSLDNNTDAKKSCDTMSEISDILKCKFDENSGNG-------------ET- 438

Query: 481 CKETGFSFPCGQ-SAAEDDEEVTESKIRAFLDEKAVELKKLQTPLYEEFRSTINAANAL- 540
             ET  S      S +ED+ E+TESKI+AFLD+KA ELKKLQTPLYEEF + +   + + 
Sbjct: 439 --ETKVSMEVDHPSYSEDENELTESKIKAFLDDKAAELKKLQTPLYEEFYNGMITCSPIC 498

Query: 541 ---GTVGTEQGKNVSNFLKLPPKCGSPSKLRGKRLSS--IDAGNCSVRQSRIKQASILQD 600
                   ++ +    FLKLPPK  SPS+    R  S   DA  CS        +     
Sbjct: 499 MESNINNNKREEAPRGFLKLPPKSRSPSQGHIGRSPSRATDAACCSKSPESGNSSG---- 558

Query: 601 RALQEIQSPQLGEWNKLLHTQSDSCTPSFSERQRKWKEELEEELKRKRAELSVRWGCAPH 647
                  +P+    +     +S+S + + SE +RKWKEEL++EL+RKR E++ + G    
Sbjct: 559 -------APKNSNASAGAEQESNSQSVALSEIERKWKEELDQELERKRREITRQAGMGSS 587

BLAST of Sgr016133 vs. TAIR 10
Match: AT1G53570.1 (mitogen-activated protein kinase kinase kinase 3 )

HSP 1 Score: 292.4 bits (747), Expect = 1.1e-78
Identity = 138/264 (52.27%), Postives = 192/264 (72.73%), Query Frame = 0

Query: 65  WRKGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRELEEEVKLLKN 124
           W+KG+ +G G FG+VY+G N + G++ A+K+V + ++  + ++    +++L +E+ LL  
Sbjct: 214 WKKGKFLGSGTFGQVYLGFNSEKGKMCAIKEVKVISDDQTSKEC---LKQLNQEINLLNQ 273

Query: 125 LSHPNIVRYLGTAREEESLNILLEFVPGGSISSLLGKFGSFPESVIRTYTKQLLLGLEYL 184
           L HPNIV+Y G+   EE+L++ LE+V GGSI  LL  +GSF E VI+ YT+Q+L GL YL
Sbjct: 274 LCHPNIVQYYGSELSEETLSVYLEYVSGGSIHKLLKDYGSFTEPVIQNYTRQILAGLAYL 333

Query: 185 HKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPEVIL- 244
           H    +HRDIKGANILVD  G IKLADFG +K V   +T+    S KG+PYWMAPEV++ 
Sbjct: 334 HGRNTVHRDIKGANILVDPNGEIKLADFGMAKHVTAFSTM---LSFKGSPYWMAPEVVMS 393

Query: 245 QTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEAKDF 304
           Q G++ + DIWS+GCT++EMAT KPPWS Q++ VAA+F IGN+K  P IP+HLS +AK+F
Sbjct: 394 QNGYTHAVDIWSLGCTILEMATSKPPWS-QFEGVAAIFKIGNSKDTPEIPDHLSNDAKNF 453

Query: 305 LLKCLQEEPNSRPTASDLLQHPFV 328
           +  CLQ  P  RPTAS LL+HPF+
Sbjct: 454 IRLCLQRNPTVRPTASQLLEHPFL 470

BLAST of Sgr016133 vs. TAIR 10
Match: AT1G53570.2 (mitogen-activated protein kinase kinase kinase 3 )

HSP 1 Score: 292.4 bits (747), Expect = 1.1e-78
Identity = 138/264 (52.27%), Postives = 192/264 (72.73%), Query Frame = 0

Query: 65  WRKGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRELEEEVKLLKN 124
           W+KG+ +G G FG+VY+G N + G++ A+K+V + ++  + ++    +++L +E+ LL  
Sbjct: 214 WKKGKFLGSGTFGQVYLGFNSEKGKMCAIKEVKVISDDQTSKEC---LKQLNQEINLLNQ 273

Query: 125 LSHPNIVRYLGTAREEESLNILLEFVPGGSISSLLGKFGSFPESVIRTYTKQLLLGLEYL 184
           L HPNIV+Y G+   EE+L++ LE+V GGSI  LL  +GSF E VI+ YT+Q+L GL YL
Sbjct: 274 LCHPNIVQYYGSELSEETLSVYLEYVSGGSIHKLLKDYGSFTEPVIQNYTRQILAGLAYL 333

Query: 185 HKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPEVIL- 244
           H    +HRDIKGANILVD  G IKLADFG +K V   +T+    S KG+PYWMAPEV++ 
Sbjct: 334 HGRNTVHRDIKGANILVDPNGEIKLADFGMAKHVTAFSTM---LSFKGSPYWMAPEVVMS 393

Query: 245 QTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEAKDF 304
           Q G++ + DIWS+GCT++EMAT KPPWS Q++ VAA+F IGN+K  P IP+HLS +AK+F
Sbjct: 394 QNGYTHAVDIWSLGCTILEMATSKPPWS-QFEGVAAIFKIGNSKDTPEIPDHLSNDAKNF 453

Query: 305 LLKCLQEEPNSRPTASDLLQHPFV 328
           +  CLQ  P  RPTAS LL+HPF+
Sbjct: 454 IRLCLQRNPTVRPTASQLLEHPFL 470

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022138818.10.0e+0094.01mitogen-activated protein kinase kinase kinase NPK1 [Momordica charantia][more]
XP_023547660.10.0e+0092.58mitogen-activated protein kinase kinase kinase NPK1 isoform X1 [Cucurbita pepo s... [more]
XP_023006461.10.0e+0092.26mitogen-activated protein kinase kinase kinase NPK1 isoform X2 [Cucurbita maxima... [more]
XP_023006458.10.0e+0092.11mitogen-activated protein kinase kinase kinase NPK1 isoform X1 [Cucurbita maxima... [more]
KAG6575002.10.0e+0091.94Mitogen-activated protein kinase kinase kinase NPK1, partial [Cucurbita argyrosp... [more]
Match NameE-valueIdentityDescription
Q405413.2e-22963.75Mitogen-activated protein kinase kinase kinase NPK1 OS=Nicotiana tabacum OX=4097... [more]
O220428.8e-20360.65Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana OX=3702... [more]
O220402.7e-17554.99Mitogen-activated protein kinase kinase kinase ANP1 OS=Arabidopsis thaliana OX=3... [more]
Q9FZ361.0e-17154.11Mitogen-activated protein kinase kinase kinase 2 OS=Arabidopsis thaliana OX=3702... [more]
Q54R821.3e-8153.58Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum OX=... [more]
Match NameE-valueIdentityDescription
A0A6J1CE750.0e+0094.01mitogen-activated protein kinase kinase kinase NPK1 OS=Momordica charantia OX=36... [more]
A0A6J1KXU20.0e+0092.26mitogen-activated protein kinase kinase kinase NPK1 isoform X2 OS=Cucurbita maxi... [more]
A0A6J1L4Z40.0e+0092.11mitogen-activated protein kinase kinase kinase NPK1 isoform X1 OS=Cucurbita maxi... [more]
A0A6J1H6610.0e+0091.79mitogen-activated protein kinase kinase kinase NPK1 isoform X2 OS=Cucurbita mosc... [more]
A0A6J1H4340.0e+0091.48mitogen-activated protein kinase kinase kinase NPK1 isoform X1 OS=Cucurbita mosc... [more]
Match NameE-valueIdentityDescription
AT3G06030.16.2e-20460.65NPK1-related protein kinase 3 [more]
AT1G09000.11.9e-17654.99NPK1-related protein kinase 1 [more]
AT1G54960.13.0e-16655.91NPK1-related protein kinase 2 [more]
AT1G53570.11.1e-7852.27mitogen-activated protein kinase kinase kinase 3 [more]
AT1G53570.21.1e-7852.27mitogen-activated protein kinase kinase kinase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 615..635
NoneNo IPR availableCOILSCoilCoilcoord: 98..125
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 65..366
e-value: 2.4E-86
score: 291.4
NoneNo IPR availablePIRSRPIRSR000620-2PIRSR000620-2coord: 71..323
e-value: 9.4E-17
score: 58.8
NoneNo IPR availablePIRSRPIRSR037993-2PIRSR037993-2coord: 29..331
e-value: 5.5E-28
score: 95.6
NoneNo IPR availablePIRSRPIRSR000556-2PIRSR000556-2coord: 68..323
e-value: 4.0E-33
score: 112.6
NoneNo IPR availablePIRSRPIRSR037993-1PIRSR037993-1coord: 29..331
e-value: 5.5E-28
score: 95.6
NoneNo IPR availablePANTHERPTHR48011CCR4-NOT TRANSCRIPTIONAL COMPLEX SUBUNIT CAF120-RELATEDcoord: 48..557
NoneNo IPR availablePANTHERPTHR48011:SF11MAP KINASE KINASE KINASEcoord: 48..557
NoneNo IPR availableCDDcd06606STKc_MAPKKKcoord: 65..327
e-value: 4.78741E-171
score: 489.724
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 65..327
e-value: 1.0E-100
score: 350.6
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 66..327
e-value: 9.3E-73
score: 244.8
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 65..327
score: 52.399696
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 189..201
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 71..94
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 65..328

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr016133.1Sgr016133.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity