Sgr015787 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr015787
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
DescriptionPalmitoyl-monogalactosyldiacylglycerol delta-7 desaturase
Locationtig00005930: 260112 .. 261970 (+)
RNA-Seq ExpressionSgr015787
SyntenySgr015787
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATAGAAGAGGTGGTGAAGCATGAACTAGAGCAAGCCCTACTAATGGAAGTCCCAAAAGAACCAAAGGAAATGGTGAAGGCTCCAAAGTCATTTAGGACGAGAAAATGGACAAAGCTAGACAAAGATATTGCTTGTTCACTTCTCATTGTTCATGTTCTTTGTGTTTTTGCACCATTTCACTTCAATTGGGGTGCATTTTGGGTTAACTTTGCGTTACAGATTATCACAGGTGTTTTAGGCATCAGTATTTCGTATCACAGAAATCTTGCACACAAGAGTTTCAAGCTCCCCAAATTACTCGAATACTTCTTTGCTTATTGTGGAGTCCATGCACTCCAGGTTTTGACACATGGATCCTTTTTCTTTTTCTTTTTCTTTTAATTACAAATGCTAAATTAATTGGTTGCTTATTTTAAGATATCTATTTTATTTTAAAAATTTTCATTTGTTTCCAAATTCTAGGGTGATCCAATCGATTGGGTGAGCACGCACAGGTGTCACCATCAATTTGTTGATACTGAAAGAGACCCACATAGTCCTATACAAGGATTCTGGTTTAGTCATATTACTTGGCTTTTTAATTCCTATGCTTTGACTAAAAGGGTTTGTCCAAAATATTTTACTGATTTTCGAAAGATAGAAAGAAGCCTCTTCATACTATTCTCAAAGCATGGGAGACCAGACAATGTTCGTGACTTGGAGAAGCAAACCTTTTATACGTTTATTCATAAAACATATCTTCTTCATCCAATTGCTCTTGCAGTTGTACTCTACGGAATTGGAGGGCTTCCCTTTTTTATATGGGGAATGGTAAAAGAAAATTACTCTCTTGCCTATTTTCTTTCAATTGCGTAAAATCTATAACTTTATTATAAGATTAGATTTGAATGATCATTCTATGATATGATATTTTCAAGATCTTATATATAACAGTGTTCAATTTCGTTTTAACCATATATTTTTCTTTAATGGAGATGAAGAGTGATTTTGAAATAATTGAAAAACTAAGGATTATTTCAAAAAAAAAAAAATAAAGTTTGAAGAAAAAAAAAAGGGTCTTAGAATTAGAGTGAAATTAAACCATGTTTTAGCCTAAGAAAAGTTAATTAATTTCTTGACCTGAAAGCGAGCTTTGTCATGCAGGGCGTGAGGATCGTTGTGGTATTACACATCACATTTATGATAAATTCTGTTTGCCATATATGGGGAAAGCAACCATGGAACACTGGCGATTTGTCAAGAAACAATTGGTTATTATTCTTTTCTTTCTCTACTTTTTTTTTTTTTTTTTGAGTTCAACAAGTGGTGAGGGTAGGGATTCGAACCTCTAATCTTTAGAAAGATTACTCGGTGCCTTAATCAATTGAACTATGCTCGATTTGACTTTTCTTTCTCTATCACTATCAAATGATATATATATACTTTGAGTTCAACACGAGATGGGAGTGGGGATTCAAACCTCTAACCTTTAGAGAGGTTACTAAGTACCTTAACGAATTGAGCTATGCTCGGTTTGACAAACTTCAATACTTATTGAATGTTATATTTGAAATATAATTAACACACTTGCAGTTTGTGTGGGTAACTATAGCCTTGTTGTATTATATCTTCATAATTTTTTTTGGTCTTCTTTTAGGTGGGTGGGTTATCTTTCACTTGGAGAAGGTTGGCATAATAACCACCATGCCTTTGAATATTCAGCTAGACATGGTCTTGAATGGTGGCAACTTGACTGTGGTTGGTATATTATTAATTTCCTTCAAGCCATTGGAGTGGCCACTGATGTGAAATTACCTTCTGAAAGCCACAAGCAAAGACTGGCTATGGACAAACCAAAAGAAGGCATTTCAAAATGA

mRNA sequence

ATGATAGAAGAGGTGGTGAAGCATGAACTAGAGCAAGCCCTACTAATGGAAGTCCCAAAAGAACCAAAGGAAATGGTGAAGGCTCCAAAGTCATTTAGGACGAGAAAATGGACAAAGCTAGACAAAGATATTGCTTGTTCACTTCTCATTGTTCATGTTCTTTGTGTTTTTGCACCATTTCACTTCAATTGGGGTGCATTTTGGGTTAACTTTGCGTTACAGATTATCACAGGTGTTTTAGGCATCAGTATTTCGTATCACAGAAATCTTGCACACAAGAGTTTCAAGCTCCCCAAATTACTCGAATACTTCTTTGCTTATTGTGGAGTCCATGCACTCCAGGGTGATCCAATCGATTGGGTGAGCACGCACAGGTGGGTGGGTTATCTTTCACTTGGAGAAGGTTGGCATAATAACCACCATGCCTTTGAATATTCAGCTAGACATGGTCTTGAATGGTGGCAACTTGACTGTGGTTGGTATATTATTAATTTCCTTCAAGCCATTGGAGTGGCCACTGATGTGAAATTACCTTCTGAAAGCCACAAGCAAAGACTGGCTATGGACAAACCAAAAGAAGGCATTTCAAAATGA

Coding sequence (CDS)

ATGATAGAAGAGGTGGTGAAGCATGAACTAGAGCAAGCCCTACTAATGGAAGTCCCAAAAGAACCAAAGGAAATGGTGAAGGCTCCAAAGTCATTTAGGACGAGAAAATGGACAAAGCTAGACAAAGATATTGCTTGTTCACTTCTCATTGTTCATGTTCTTTGTGTTTTTGCACCATTTCACTTCAATTGGGGTGCATTTTGGGTTAACTTTGCGTTACAGATTATCACAGGTGTTTTAGGCATCAGTATTTCGTATCACAGAAATCTTGCACACAAGAGTTTCAAGCTCCCCAAATTACTCGAATACTTCTTTGCTTATTGTGGAGTCCATGCACTCCAGGGTGATCCAATCGATTGGGTGAGCACGCACAGGTGGGTGGGTTATCTTTCACTTGGAGAAGGTTGGCATAATAACCACCATGCCTTTGAATATTCAGCTAGACATGGTCTTGAATGGTGGCAACTTGACTGTGGTTGGTATATTATTAATTTCCTTCAAGCCATTGGAGTGGCCACTGATGTGAAATTACCTTCTGAAAGCCACAAGCAAAGACTGGCTATGGACAAACCAAAAGAAGGCATTTCAAAATGA

Protein sequence

MIEEVVKHELEQALLMEVPKEPKEMVKAPKSFRTRKWTKLDKDIACSLLIVHVLCVFAPFHFNWGAFWVNFALQIITGVLGISISYHRNLAHKSFKLPKLLEYFFAYCGVHALQGDPIDWVSTHRWVGYLSLGEGWHNNHHAFEYSARHGLEWWQLDCGWYIINFLQAIGVATDVKLPSESHKQRLAMDKPKEGISK
Homology
BLAST of Sgr015787 vs. NCBI nr
Match: XP_022153632.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Momordica charantia] >XP_022153633.1 palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Momordica charantia])

HSP 1 Score: 251.1 bits (640), Expect = 7.7e-63
Identity = 139/338 (41.12%), Postives = 167/338 (49.41%), Query Frame = 0

Query: 1   MIEEVVKHELEQALLMEVPK-EPKEMVKAPKSF-RTRKWTKLDKDIACSLLIVHVLCVFA 60
           M+E+  K EL+QA LME PK EPKEM   PKSF + +KWTKLDKD+ACSL  VH+L +FA
Sbjct: 6   MLEKWCKPELKQATLMEAPKQEPKEMAIPPKSFGKRKKWTKLDKDVACSLFFVHLLSMFA 65

Query: 61  PFHFNWGAFWVNFALQIITGVLGISISYHRNLAHKSFKLPKLLEYFFAYCGVHALQGDPI 120
           PF+FNW AFW+ FAL + TG+ GIS+SYHRNLAHKSF LPK LEY FAYCG HALQGDPI
Sbjct: 66  PFYFNWRAFWIAFALYVATGLFGISVSYHRNLAHKSFTLPKWLEYLFAYCGAHALQGDPI 125

Query: 121 DWVSTHR----------------------------------------------------- 180
           DWV+THR                                                     
Sbjct: 126 DWVNTHRCHHRFVDTERDPHSPIQGFWFSHITWLLDSYSLTKRVCPKYFTDFQKVERNIF 185

Query: 181 ------------------------------------------------------------ 196
                                                                       
Sbjct: 186 VMFSKHGRPDNVGDLEKQTFYRFIHKTYFLHPIILAIILYAYGGAPFLIWGMGVRIVVVL 245

BLAST of Sgr015787 vs. NCBI nr
Match: KAG6581931.1 (Delta-9 acyl-lipid desaturase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 249.2 bits (635), Expect = 2.9e-62
Identity = 122/242 (50.41%), Postives = 141/242 (58.26%), Query Frame = 0

Query: 20  KEPKEMVKAPKSFRTRKWTKLDKDIACSLLIVHVLCVFAPFHFNWGAFWVNFALQIITGV 79
           +E K M K    F +RKWT  +K +  +  I+H+LC+FAPF F W AFWV  AL +I+G+
Sbjct: 6   QESKHMAKPEMPFCSRKWTNREKLVTVTFSIIHILCIFAPFQFTWNAFWVAVALYVISGL 65

Query: 80  LGISISYHRNLAHKSFKLPKLLEYFFAYCGVHALQGDPIDWVSTH--------------- 139
            GI+ISYHRNL+H+SF LPK LEY FAYCG HA QGDPIDWVSTH               
Sbjct: 66  FGITISYHRNLSHRSFNLPKWLEYLFAYCGTHAFQGDPIDWVSTHRCHHQNADTERDPHS 125

Query: 140 -------------------------------------------------RWVGYLSLGEG 198
                                                            RW+  +S GE 
Sbjct: 126 PTQGFWFSYLIWFLDSITLTNKVCPEYFIDRKDTKRGVFTLVLKVLCVLRWLSLMSFGEA 185

BLAST of Sgr015787 vs. NCBI nr
Match: XP_031739648.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic isoform X2 [Cucumis sativus])

HSP 1 Score: 233.8 bits (595), Expect = 1.3e-57
Identity = 117/241 (48.55%), Postives = 136/241 (56.43%), Query Frame = 0

Query: 20  KEPKEMVKAPKSFRTRKWTKLDKDIACSLLIVHVLCVFAPFHFNWGAFWVNFALQIITGV 79
           +E  E VK   S   R+WT +DK IAC L  +H LC+FAPF+F W AFWV   L  ITG+
Sbjct: 6   QELNERVKPIMSPTKREWTNIDKTIACLLFFLHALCIFAPFNFTWNAFWVALILYSITGL 65

Query: 80  LGISISYHRNLAHKSFKLPKLLEYFFAYCGVHALQGDPIDWVSTHR-------------- 139
            GI+ISYHRNL+HKSF+LPK LEY FAYCG HALQGDP+DWVSTHR              
Sbjct: 66  FGITISYHRNLSHKSFQLPKWLEYLFAYCGAHALQGDPMDWVSTHRCHHQFVDTDKDPHD 125

Query: 140 ----------------------------------------------------------WV 189
                                                                     WV
Sbjct: 126 RNQGFWFSHINWAFDSYHLTKKCVRIVMLYHVIFMVNSVCHKWGKKQWSTTDLSRNNWWV 185

BLAST of Sgr015787 vs. NCBI nr
Match: XP_022979557.1 (delta-9 acyl-lipid desaturase 1-like [Cucurbita maxima])

HSP 1 Score: 232.3 bits (591), Expect = 3.7e-57
Identity = 133/327 (40.67%), Postives = 155/327 (47.40%), Query Frame = 0

Query: 13  ALLMEVPK-EPKEMVKAPKSFRTRKWTKLDKDIACSLLIVHVLCVFAPFHFNWGAFWVNF 72
           ALL +  K EPKEMVK P+SF  RKWTKLDK +A  +L++H+LC+FAPFHF W AF V  
Sbjct: 15  ALLTDASKQEPKEMVKPPRSFWRRKWTKLDKRVAHVILLLHLLCIFAPFHFTWPAFRVAV 74

Query: 73  ALQIITGVLGISISYHRNLAHKSFKLPKLLEYFFAYCGVHALQGDPIDWVSTHR------ 132
            LQIITG+ G++ISYHRNLAHKSF++PK LEY FAYCGVHALQGDPIDWVSTHR      
Sbjct: 75  TLQIITGLFGMTISYHRNLAHKSFQIPKWLEYLFAYCGVHALQGDPIDWVSTHRCHHQFV 134

Query: 133 ------------------------------------------------------------ 192
                                                                       
Sbjct: 135 DTERDPHSPIQGFWMSHIMWLFDSYTLTDKVSPKYSSNLQKIEKNMFVYFSKYRKPENVG 194

Query: 193 ------------------------------------------------------------ 198
                                                                       
Sbjct: 195 DLERQTFYRFIHKTYLLHPIALAAILYTVGGVPFLIWGMCVRIVVIFHSTFMVNSVCHMW 254

BLAST of Sgr015787 vs. NCBI nr
Match: XP_038875096.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 229.2 bits (583), Expect = 3.1e-56
Identity = 134/332 (40.36%), Postives = 156/332 (46.99%), Query Frame = 0

Query: 1   MIEEVVKHELEQALLMEVPK--EPKEMVKAPKSFRTRKWTKLDKDIACSLLIVHVLCVFA 60
           ++E+ VK +  + LLM+ PK  EPKEMV AP+ F  RKWT LDK +A  +L+VH+LC+FA
Sbjct: 5   LLEKEVKPQPHKPLLMDEPKQEEPKEMVNAPRPFWRRKWTNLDKRVAYIILLVHLLCIFA 64

Query: 61  PFHFNWGAFWVNFALQIITGVLGISISYHRNLAHKSFKLPKLLEYFFAYCGVHALQGDPI 120
           PF FNW AF V  ALQIITG+ G+++SYHRNLAHKSFKLPK LEY FAYCGV ALQGDPI
Sbjct: 65  PFQFNWSAFRVAVALQIITGLFGMTVSYHRNLAHKSFKLPKWLEYLFAYCGVQALQGDPI 124

Query: 121 DWVSTHR----------------------------------------------------- 180
           DWVSTHR                                                     
Sbjct: 125 DWVSTHRCHHQYVDTEKDPHSPIQGFWNSHMMWLFDSYILTNKVCPKYSSDLQKIERNMF 184

Query: 181 ------------------------------------------------------------ 190
                                                                       
Sbjct: 185 VFFSKHGKPDNVGDLEKQRFYRFIHKTYVLHPIALAVILYNIGGVPFLIWGMCVRIVVIF 244

BLAST of Sgr015787 vs. ExPASy Swiss-Prot
Match: Q949X0 (Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ADS3 PE=1 SV=2)

HSP 1 Score: 186.8 bits (473), Expect = 2.3e-46
Identity = 103/272 (37.87%), Postives = 124/272 (45.59%), Query Frame = 0

Query: 35  RKWTKLDKDIACSLLIVHVLCVFAPFHFNWGAFWVNFALQIITGVLGISISYHRNLAHKS 94
           R+W  +D      +L +H+L + APF FNW A  V F L I+TG+LGI++S+HRNL+HK+
Sbjct: 96  REWKAMDFGAVAVVLSMHLLSLLAPFQFNWRAVSVAFGLYIVTGLLGITLSFHRNLSHKA 155

Query: 95  FKLPKLLEYFFAYCGVHALQGDPIDWVSTHR----------------------------- 154
           FKLPK LEY FAYCG  ALQG+PIDWVSTHR                             
Sbjct: 156 FKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDPHSPLDGFWFSHMNWMFD 215

Query: 155 ------------------------------------------------------------ 188
                                                                       
Sbjct: 216 TNTITQRCGEPNNVGDLEKQPFYRFLRTTYILHPLALAVALYAMGGFPFIVWGMGVRIVW 275

BLAST of Sgr015787 vs. ExPASy Swiss-Prot
Match: O65797 (Delta-9 acyl-lipid desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=ADS1 PE=2 SV=1)

HSP 1 Score: 175.3 bits (443), Expect = 7.0e-43
Identity = 102/284 (35.92%), Postives = 125/284 (44.01%), Query Frame = 0

Query: 24  EMVKAPKSFRTRKWTKLDKDIACSLLIVHVLCVFAPFHFNWGAFWVNFALQIITGVLGIS 83
           EM +  ++   RKW +LD   A + L VH LC+ APF+F W A  V   +  + G LGI+
Sbjct: 21  EMGRKKRAMWERKWKRLDIVKAFASLFVHFLCLLAPFNFTWPALRVALIVYTVGG-LGIT 80

Query: 84  ISYHRNLAHKSFKLPKLLEYFFAYCGVHALQGDPIDWVSTHR------------------ 143
           +SYHRNLAH+SFK+PK LEYFFAYCG+ A+QGDPIDWVSTHR                  
Sbjct: 81  VSYHRNLAHRSFKVPKWLEYFFAYCGLLAIQGDPIDWVSTHRYHHQFTDSDRDPHSPNEG 140

Query: 144 ------------------------------------------------------------ 189
                                                                       
Sbjct: 141 FWFSHLLWLFDTGYLVEKCGRRTNVEDLKRQWYYKFLQRTVLYHILTFGFLLYYFGGLSF 200

BLAST of Sgr015787 vs. ExPASy Swiss-Prot
Match: P0DOW2 (Acyl-CoA C20 Delta5-desaturase OS=Anemone leveillei OX=212809 GN=AL10 PE=1 SV=1)

HSP 1 Score: 170.2 bits (430), Expect = 2.3e-41
Identity = 103/306 (33.66%), Postives = 131/306 (42.81%), Query Frame = 0

Query: 1   MIEEVVKHELEQALLMEVPKEPKEMVKAPKSFRTRKWTKLDKDIACSLLIVHVLCVFAPF 60
           M E     E ++    EVP +     K  K    RKW   D      +  +HVLC+FAP 
Sbjct: 8   MQETTAAAEEDRLPCSEVPVKE----KTKKITWVRKWNSTDVFHILFIGGLHVLCLFAPS 67

Query: 61  HFNWGAFWVNFALQIITGVLGISISYHRNLAHKSFKLPKLLEYFFAYCGVHALQGDPIDW 120
            F+W +FWV FAL  I GV G ++S+HRNL H+SFKLPK LEYFFAY G+HALQGDP+ W
Sbjct: 68  TFSWKSFWVCFALYAICGVFGTTLSFHRNLTHRSFKLPKYLEYFFAYVGLHALQGDPVWW 127

Query: 121 VSTHRW------------------------------------------------------ 180
           VSTHR+                                                      
Sbjct: 128 VSTHRYHHKYTDTYLDPHSPIEGFWFCHIFWLFDSKYIIEKCGRYENAGDLMKQSYYRFL 187

Query: 181 ------------------------------------------------------------ 188
                                                                       
Sbjct: 188 ERTFVYHVYLQAALLYLFGGFPFIVWGMAVRTILGFHLSWLVNSVCHRWGNRPWNTGDLS 247

BLAST of Sgr015787 vs. ExPASy Swiss-Prot
Match: P0DOW3 (Acyl-CoA 5-desaturase AL21 OS=Anemone leveillei OX=212809 GN=AL21 PE=1 SV=1)

HSP 1 Score: 169.5 bits (428), Expect = 3.9e-41
Identity = 99/300 (33.00%), Postives = 133/300 (44.33%), Query Frame = 0

Query: 13  ALLMEVPKEPKEMVKAPKSFRTRK--WTKL--DKDIACSLLI--VHVLCVFAPFHFNWGA 72
           AL  E+ ++       P   +T+K  W ++    DI   +L+  +HVLC+ APF F+W A
Sbjct: 7   ALQSELKQDKFPSSDVPVKDKTKKITWVRVWNSTDIFHIILVGGLHVLCLSAPFTFSWSA 66

Query: 73  FWVNFALQIITGVLGISISYHRNLAHKSFKLPKLLEYFFAYCGVHALQGDPIDWVSTHRW 132
           FW++  L  + GV G ++SYHRNL H+SFKLPK LEYFFAY G+HALQGDP+ WVSTHR+
Sbjct: 67  FWLSLTLYAVCGVFGTTLSYHRNLTHRSFKLPKYLEYFFAYVGLHALQGDPVWWVSTHRY 126

Query: 133 ------------------------------------------------------------ 188
                                                                       
Sbjct: 127 HHKFTDTYLDPHSPIEGFWFSHIFWLFDSKYILEECGRYENAGDLLKQSYYRFLERTFVF 186

BLAST of Sgr015787 vs. ExPASy Swiss-Prot
Match: Q9FV68 (Icosanoyl-CoA 5-desaturase (Fragment) OS=Limnanthes douglasii OX=28973 PE=1 SV=1)

HSP 1 Score: 167.5 bits (423), Expect = 1.5e-40
Identity = 103/301 (34.22%), Postives = 129/301 (42.86%), Query Frame = 0

Query: 7   KHELEQALLMEVPKEPKEMVKAPKSFRTRKWTKLDKDIACSLLIVHVLCVFAPFHFNWGA 66
           K +LE+    EV  E K      K F  RKW   D   A +LL++H L   APF+F+W A
Sbjct: 56  KDDLERFRTSEVVLERK-----AKGFWRRKWNPRDIQNAVTLLVLHALAAMAPFYFSWDA 115

Query: 67  FWVNF-ALQIITGVLGISISYHRNLAHKSFKLPKLLEYFFAYCGVHALQGDPIDWVSTHR 126
           FW++F  L   +GVLGI++ +HR L H  FKLPKL+EYFFAYCG  ALQGDP++WVS HR
Sbjct: 116 FWISFILLGFASGVLGITLCFHRCLTHGGFKLPKLVEYFFAYCGSLALQGDPMEWVSNHR 175

Query: 127 W----------------------------------------------------------- 186
           +                                                           
Sbjct: 176 YHHQFVDTERDVHSPTQGFWFCHIGWVLDKDLFVEKRGGRRNNVNDLKKQAFYRFLQKTY 235

BLAST of Sgr015787 vs. ExPASy TrEMBL
Match: A0A6J1DHZ7 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111021091 PE=3 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 3.7e-63
Identity = 139/338 (41.12%), Postives = 167/338 (49.41%), Query Frame = 0

Query: 1   MIEEVVKHELEQALLMEVPK-EPKEMVKAPKSF-RTRKWTKLDKDIACSLLIVHVLCVFA 60
           M+E+  K EL+QA LME PK EPKEM   PKSF + +KWTKLDKD+ACSL  VH+L +FA
Sbjct: 6   MLEKWCKPELKQATLMEAPKQEPKEMAIPPKSFGKRKKWTKLDKDVACSLFFVHLLSMFA 65

Query: 61  PFHFNWGAFWVNFALQIITGVLGISISYHRNLAHKSFKLPKLLEYFFAYCGVHALQGDPI 120
           PF+FNW AFW+ FAL + TG+ GIS+SYHRNLAHKSF LPK LEY FAYCG HALQGDPI
Sbjct: 66  PFYFNWRAFWIAFALYVATGLFGISVSYHRNLAHKSFTLPKWLEYLFAYCGAHALQGDPI 125

Query: 121 DWVSTHR----------------------------------------------------- 180
           DWV+THR                                                     
Sbjct: 126 DWVNTHRCHHRFVDTERDPHSPIQGFWFSHITWLLDSYSLTKRVCPKYFTDFQKVERNIF 185

Query: 181 ------------------------------------------------------------ 196
                                                                       
Sbjct: 186 VMFSKHGRPDNVGDLEKQTFYRFIHKTYFLHPIILAIILYAYGGAPFLIWGMGVRIVVVL 245

BLAST of Sgr015787 vs. ExPASy TrEMBL
Match: A0A6J1IP09 (delta-9 acyl-lipid desaturase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111479233 PE=3 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 1.8e-57
Identity = 133/327 (40.67%), Postives = 155/327 (47.40%), Query Frame = 0

Query: 13  ALLMEVPK-EPKEMVKAPKSFRTRKWTKLDKDIACSLLIVHVLCVFAPFHFNWGAFWVNF 72
           ALL +  K EPKEMVK P+SF  RKWTKLDK +A  +L++H+LC+FAPFHF W AF V  
Sbjct: 15  ALLTDASKQEPKEMVKPPRSFWRRKWTKLDKRVAHVILLLHLLCIFAPFHFTWPAFRVAV 74

Query: 73  ALQIITGVLGISISYHRNLAHKSFKLPKLLEYFFAYCGVHALQGDPIDWVSTHR------ 132
            LQIITG+ G++ISYHRNLAHKSF++PK LEY FAYCGVHALQGDPIDWVSTHR      
Sbjct: 75  TLQIITGLFGMTISYHRNLAHKSFQIPKWLEYLFAYCGVHALQGDPIDWVSTHRCHHQFV 134

Query: 133 ------------------------------------------------------------ 192
                                                                       
Sbjct: 135 DTERDPHSPIQGFWMSHIMWLFDSYTLTDKVSPKYSSNLQKIEKNMFVYFSKYRKPENVG 194

Query: 193 ------------------------------------------------------------ 198
                                                                       
Sbjct: 195 DLERQTFYRFIHKTYLLHPIALAAILYTVGGVPFLIWGMCVRIVVIFHSTFMVNSVCHMW 254

BLAST of Sgr015787 vs. ExPASy TrEMBL
Match: A0A6J1DJP0 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111021101 PE=3 SV=1)

HSP 1 Score: 227.6 bits (579), Expect = 4.4e-56
Identity = 127/311 (40.84%), Postives = 144/311 (46.30%), Query Frame = 0

Query: 16  MEVPK-EPKEMVKAPKSFRTRKWTKLDKDIACSLLIVHVLCVFAPFHFNWGAFWVNFALQ 75
           ME  K EPKEM+K P+ +  RKWTKLDKDIACSL +VH+LC+ APF FNW AFWV F L 
Sbjct: 1   MEASKQEPKEMMKPPRPYERRKWTKLDKDIACSLFLVHLLCILAPFQFNWSAFWVAFVLY 60

Query: 76  IITGVLGISISYHRNLAHKSFKLPKLLEYFFAYCGVHALQGDPIDWVSTHR--------- 135
           I+TG+ G+++SYHRNLAHKSFKLPK LEY FAYCGV ALQGDP+DWVSTHR         
Sbjct: 61  IVTGLFGMTLSYHRNLAHKSFKLPKWLEYLFAYCGVQALQGDPMDWVSTHRCHHQFVDTE 120

Query: 136 ------------------------------------------------------------ 185
                                                                       
Sbjct: 121 RDPHSPIRGFWFSHILWLFDSYNLTKKVCPKYFSNFQKLERNIFVLFSKHGKPENVADLE 180

BLAST of Sgr015787 vs. ExPASy TrEMBL
Match: A0A6J1GXC1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111458012 PE=3 SV=1)

HSP 1 Score: 227.6 bits (579), Expect = 4.4e-56
Identity = 131/327 (40.06%), Postives = 153/327 (46.79%), Query Frame = 0

Query: 13  ALLMEVPK-EPKEMVKAPKSFRTRKWTKLDKDIACSLLIVHVLCVFAPFHFNWGAFWVNF 72
           ALL +  K EPKEMVK P+SF  RKWTKLDK +A  +L++H+LC+ APFHF W AF V  
Sbjct: 15  ALLTDASKQEPKEMVKPPRSFWRRKWTKLDKRVAHVILLLHLLCILAPFHFTWPAFRVAV 74

Query: 73  ALQIITGVLGISISYHRNLAHKSFKLPKLLEYFFAYCGVHALQGDPIDWVSTHR------ 132
            LQIITG+ G++ISYHRNLAHKSF++PK LEY FAYCGVHALQGDPIDWVSTHR      
Sbjct: 75  TLQIITGLFGMTISYHRNLAHKSFQIPKWLEYLFAYCGVHALQGDPIDWVSTHRCHHQFV 134

Query: 133 ------------------------------------------------------------ 192
                                                                       
Sbjct: 135 DTERDPHSPIQGFWMSHIMWLFDSYTLTDKVSPKYSSDLQKIEKNMFVYFSKYRKPENVG 194

Query: 193 ------------------------------------------------------------ 198
                                                                       
Sbjct: 195 DLERQTFYRFIHKTYLLHPMALAAILYTVGGFPFLIWGMCVRIVVIFHSTFMVNSVCHMW 254

BLAST of Sgr015787 vs. ExPASy TrEMBL
Match: A0A6J1DIY2 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111020911 PE=3 SV=1)

HSP 1 Score: 224.2 bits (570), Expect = 4.9e-55
Identity = 124/312 (39.74%), Postives = 147/312 (47.12%), Query Frame = 0

Query: 14  LLMEVPKEPKEMVKAPKSFRTRKWTKLDKDIACSLLIVHVLCVFAPFHFNWGAFWVNFAL 73
           ++M+  KEPK +VK+P SF  RKWTK+DK  AC LL +HVLC+FAPF FNW AFW+ FAL
Sbjct: 1   MVMDASKEPKIIVKSPMSFGRRKWTKMDKTNACILLFLHVLCIFAPFQFNWSAFWIAFAL 60

Query: 74  QIITGVLGISISYHRNLAHKSFKLPKLLEYFFAYCGVHALQGDPIDWVSTHR-------- 133
            IITG+ GI+ISYHRNL+H+SFKLPK LEY FAYCGV +LQGDPI WVSTHR        
Sbjct: 61  YIITGLFGITISYHRNLSHRSFKLPKWLEYLFAYCGVQSLQGDPIYWVSTHRYHHQFVDT 120

Query: 134 -----------WVGY--------------------------------------------- 189
                      W  Y                                             
Sbjct: 121 EKDPHSPIQGFWFSYIFWIFDSNTLTNKMSNFQERPDRSTSVMIMKHNRLDNVGDLKKQT 180

BLAST of Sgr015787 vs. TAIR 10
Match: AT3G15850.1 (fatty acid desaturase 5 )

HSP 1 Score: 186.8 bits (473), Expect = 1.7e-47
Identity = 103/272 (37.87%), Postives = 124/272 (45.59%), Query Frame = 0

Query: 35  RKWTKLDKDIACSLLIVHVLCVFAPFHFNWGAFWVNFALQIITGVLGISISYHRNLAHKS 94
           R+W  +D      +L +H+L + APF FNW A  V F L I+TG+LGI++S+HRNL+HK+
Sbjct: 96  REWKAMDFGAVAVVLSMHLLSLLAPFQFNWRAVSVAFGLYIVTGLLGITLSFHRNLSHKA 155

Query: 95  FKLPKLLEYFFAYCGVHALQGDPIDWVSTHR----------------------------- 154
           FKLPK LEY FAYCG  ALQG+PIDWVSTHR                             
Sbjct: 156 FKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDPHSPLDGFWFSHMNWMFD 215

Query: 155 ------------------------------------------------------------ 188
                                                                       
Sbjct: 216 TNTITQRCGEPNNVGDLEKQPFYRFLRTTYILHPLALAVALYAMGGFPFIVWGMGVRIVW 275

BLAST of Sgr015787 vs. TAIR 10
Match: AT1G06080.1 (delta 9 desaturase 1 )

HSP 1 Score: 175.3 bits (443), Expect = 5.0e-44
Identity = 102/284 (35.92%), Postives = 125/284 (44.01%), Query Frame = 0

Query: 24  EMVKAPKSFRTRKWTKLDKDIACSLLIVHVLCVFAPFHFNWGAFWVNFALQIITGVLGIS 83
           EM +  ++   RKW +LD   A + L VH LC+ APF+F W A  V   +  + G LGI+
Sbjct: 21  EMGRKKRAMWERKWKRLDIVKAFASLFVHFLCLLAPFNFTWPALRVALIVYTVGG-LGIT 80

Query: 84  ISYHRNLAHKSFKLPKLLEYFFAYCGVHALQGDPIDWVSTHR------------------ 143
           +SYHRNLAH+SFK+PK LEYFFAYCG+ A+QGDPIDWVSTHR                  
Sbjct: 81  VSYHRNLAHRSFKVPKWLEYFFAYCGLLAIQGDPIDWVSTHRYHHQFTDSDRDPHSPNEG 140

Query: 144 ------------------------------------------------------------ 189
                                                                       
Sbjct: 141 FWFSHLLWLFDTGYLVEKCGRRTNVEDLKRQWYYKFLQRTVLYHILTFGFLLYYFGGLSF 200

BLAST of Sgr015787 vs. TAIR 10
Match: AT2G31360.1 (16:0delta9 desaturase 2 )

HSP 1 Score: 161.8 bits (408), Expect = 5.7e-40
Identity = 95/289 (32.87%), Postives = 119/289 (41.18%), Query Frame = 0

Query: 19  PKEPKEMVKAPKSFRTRKWTKLDKDIACSLLIVHVLCVFAPFHFNWGAFWVNFALQIITG 78
           P   +E  K    F  R+W +LD     +   VH L + APF+F W A WV F    I G
Sbjct: 18  PAAVEEKKKRRWVFWDRRWRRLDYVKFSASFTVHSLALLAPFYFTWSALWVTFLFYTIGG 77

Query: 79  VLGISISYHRNLAHKSFKLPKLLEYFFAYCGVHALQGDPIDWVSTHR------------- 138
            LGI++SYHRNLAH+SFK+PK LEY  AYC + A+QGDPIDWVSTHR             
Sbjct: 78  -LGITVSYHRNLAHRSFKVPKWLEYLLAYCALLAIQGDPIDWVSTHRYHHQFTDSERDPH 137

Query: 139 ------------------------------------------------------------ 189
                                                                       
Sbjct: 138 SPKEGFWFSHLLWIYDSAYLVSKCGRRANVEDLKRQWFYRFLQKTVLFHILGLGFFLFYL 197

BLAST of Sgr015787 vs. TAIR 10
Match: AT3G15870.1 (Fatty acid desaturase family protein )

HSP 1 Score: 158.3 bits (399), Expect = 6.3e-39
Identity = 96/283 (33.92%), Postives = 120/283 (42.40%), Query Frame = 0

Query: 25  MVKAPKSFRTRKWTKLDKDIACSLLIVHVLCVFAPFHFNWGAFWVNFALQIITGVLGISI 84
           +V+  +SF  R WT  D       +  H+L + APF+F+W AFWV   L  I G+  I++
Sbjct: 79  VVRTKRSFWERSWTSWDVSKLVIFVGTHLLSLLAPFYFSWEAFWVFPWLVFINGIC-ITL 138

Query: 85  SYHRNLAHKSFKLPKLLEYFFAYCGVHALQGDPIDWVSTHR------------------- 144
           SYHRNL+H+SF LPK LEY FAY GV A QGDPI+WVS HR                   
Sbjct: 139 SYHRNLSHRSFDLPKWLEYLFAYGGVLAFQGDPIEWVSNHRYHHKHCETQRDPHSPTQGF 198

Query: 145 ------------------------------------------------------------ 189
                                                                       
Sbjct: 199 WFSHMAWIFDTSSILENCGGEENVDDLVRQPFYRFLQRTVLLHMMAYSFLFYFCGGMPLL 258

BLAST of Sgr015787 vs. TAIR 10
Match: AT1G06090.1 (Fatty acid desaturase family protein )

HSP 1 Score: 157.1 bits (396), Expect = 1.4e-38
Identity = 94/277 (33.94%), Postives = 120/277 (43.32%), Query Frame = 0

Query: 30  KSFRTRKWTKLDKDIACSLLIVHVLCVFAPFHFNWGAFWVNFALQIITGVLGISISYHRN 89
           ++F  RKWT++D   A ++  VH+LC+ APF++ W A      L I+T  L I+ SYHRN
Sbjct: 21  RAFFFRKWTRIDIARASAVGAVHLLCLLAPFNYKWEALRFGVILAIVTS-LSITFSYHRN 80

Query: 90  LAHKSFKLPKLLEYFFAYCGVHALQGDPIDWVSTHR------------------------ 149
           L HKSFKLPK LEY FAY  + ALQG PIDWVSTHR                        
Sbjct: 81  LTHKSFKLPKWLEYPFAYSALFALQGHPIDWVSTHRFHHQFTDSDRDPHSPIEGFWFSHV 140

Query: 150 ------------------------------------------------------------ 188
                                                                       
Sbjct: 141 FWIFDTSYIREKCGGRDNVMDLKQQWFYRFLRNTIGLHILTFWTLVYLWGGLPYLTCGVG 200

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022153632.17.7e-6341.12palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [M... [more]
KAG6581931.12.9e-6250.41Delta-9 acyl-lipid desaturase 2, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_031739648.11.3e-5748.55palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic isoform... [more]
XP_022979557.13.7e-5740.67delta-9 acyl-lipid desaturase 1-like [Cucurbita maxima][more]
XP_038875096.13.1e-5640.36palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [B... [more]
Match NameE-valueIdentityDescription
Q949X02.3e-4637.87Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arab... [more]
O657977.0e-4335.92Delta-9 acyl-lipid desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=ADS1 PE=2 SV=... [more]
P0DOW22.3e-4133.66Acyl-CoA C20 Delta5-desaturase OS=Anemone leveillei OX=212809 GN=AL10 PE=1 SV=1[more]
P0DOW33.9e-4133.00Acyl-CoA 5-desaturase AL21 OS=Anemone leveillei OX=212809 GN=AL21 PE=1 SV=1[more]
Q9FV681.5e-4034.22Icosanoyl-CoA 5-desaturase (Fragment) OS=Limnanthes douglasii OX=28973 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1DHZ73.7e-6341.12palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
A0A6J1IP091.8e-5740.67delta-9 acyl-lipid desaturase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111479233... [more]
A0A6J1DJP04.4e-5640.84palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
A0A6J1GXC14.4e-5640.06palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
A0A6J1DIY24.9e-5539.74palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
Match NameE-valueIdentityDescription
AT3G15850.11.7e-4737.87fatty acid desaturase 5 [more]
AT1G06080.15.0e-4435.92delta 9 desaturase 1 [more]
AT2G31360.15.7e-4032.8716:0delta9 desaturase 2 [more]
AT3G15870.16.3e-3933.92Fatty acid desaturase family protein [more]
AT1G06090.11.4e-3833.94Fatty acid desaturase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR11351:SF94BNAC05G37460D PROTEINcoord: 32..126
NoneNo IPR availablePANTHERPTHR11351:SF94BNAC05G37460D PROTEINcoord: 126..189
IPR015876Acyl-CoA desaturasePANTHERPTHR11351ACYL-COA DESATURASEcoord: 32..126
coord: 126..189
IPR015876Acyl-CoA desaturaseCDDcd03505Delta9-FADS-likecoord: 64..176
e-value: 2.14871E-38
score: 128.442

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr015787.1Sgr015787.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
molecular_function GO:0016717 oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water