Sgr015778 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr015778
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
DescriptionPhospholipase D
Locationtig00005930: 198177 .. 200062 (+)
RNA-Seq ExpressionSgr015778
SyntenySgr015778
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTCATATTCTGCTCCATGGCACACTTCATGTCACCATTTATGAAGTGGATAGGCTGCATTCCAGTGGCCGTAATATCTTCAAGAAGGTAACCTTTTGTTTTAGTTCCGTCCACTCAACTATTATTTATTCTGTTATATCGTGTTAAATCAATTAAAATTTGTCTGGCATGCCGCTTTATCACTGAATTTATAACTCAACTTATATTTTTTTGTGTAGTTGGTTGAAAATGTAGAAGAAGCAGTTGGGTTTGGCCGAGGAGTAACTAAGTTATATGCAACCATTGATCTAGAGAAGGCCAGAGTTGGAAGAACAAGAATACTAGAGAAAGAACATTCTAACCCAAAATGGTATGAGACTTTCCATATCTACTGTGCTCATATGGCTTCCAACGTCATCTTCACTGTCAAGGATGACAATCCCATCGGAGCAACCTTAATTGGCAGAGCATATTTGTCTGTCCAAGACATCATACACGGGGAAGAAGTGGACAAATGGGTTCCAATCTTGGATGAACGCAAGAACCCCATTCGTGGGGAGCCCAAGATTCATGTCAAATTACAATATTTTGATGTCACAAAGGATCGAAACTGGGGTCGAGGCATCAAAACCCCCAAGTTTCCTGGTGTTCCCTACACTTACTTCTCTCAGAGACGAGGCTGTAAGGTAACTTTGTATCAAGATGCTCATGTTCCAGACAACTTTATTCCCAGAATCCCTCTTGCCGGAGGCAAGTTTTATGAGCCACACCGATGTTGGGAAGATGTTTTTGATGCAATAACTAATGCAAAACACTTGATTTACATTACTGGATGGTCTGTTTACACTGAGATTTCTTTGGTGAGAGATACAAGAAGACCAAAGCCTGGTGGAGACATCATACTCGGCGAATTGCTTAAGAGAAAGGCAAGTGAAGGTGTTAGAGTTCTTTTACTTGTTTGGGATGATAAGACTTCGGTGCCGGTGTTGAAGAAGGATGGGCTGATGGCAACTCATGATGAAGATACTGCAAAGTACTTTGAGAATACTGATGTGCACTGTGTGTTGTGCCCTCGCAATCCTGATGATGGTGCGAACGTTATTCAGGACATAGCTGTTGGGACGATGTTCACTCATCACCAGAAGATTGTTGTGGTGGACAGCGCCTTGCCTAGTGGGGATCCAAACAGAAGGAGAATAGTGAGTTTTGTTGGTGGGATTGATCTCTGTGATGGCAGGTACGATACCCCATTTCACTCCCTTTTCAGGACGTTGGATTCTGCTCATCATGATGATTTCCATCAGCCAAACTTCAATGGTACTTCCATAAACAAAGGTGGTCCGAGAGAGCCGTGGCACGACATCCATTCCCGGCTCGAAGGACCTGTCGCATGGGATGTTATGTACAACTTTGAACAGAGATGGAAGAAGCAAGGTGGGAAGGATTTGCTTGTTAAACTGAGAGATCTAGATGACATTTTCATCACCCCATCGCCTGTGATGTTCCCAGATGACTTTGACAGTTGGAATGTTCAAGTTTTCCGGTCGATCGATGGCGGGGCGGCTTTCGGCTTCCCTGAGACGCCTGAAGATGCAGCCAAAGCTGGGCTTATAAGTGGAAAGGATAACATTATTGATCGAAGCATTCAGGATGCTTACATTCATGCTATCCGACGGGCAAAGAATTTCATATACATTGAAAACCAGTATTTCCTTGGAAGCTCGTTTGAGTGGAGAGGGGATGATGTGAAACCTGAGATGATCAATGCCTTGCATTTAATCCCAAAGGAACTTTCACTTAAGATTGTGAGCAAGATTGAAGCTAGAGAGAGGTTCACCGTGTACATTGTCGTCCCGATGTGGCCAGAAGGGCTGCCGGAGGGGGAACAGTTCAGGCGATATTAG

mRNA sequence

ATGGCTCATATTCTGCTCCATGGCACACTTCATGTCACCATTTATGAAGTGGATAGGCTGCATTCCAGTGGCCGTAATATCTTCAAGAAGTTGGTTGAAAATGTAGAAGAAGCAGTTGGGTTTGGCCGAGGAGTAACTAAGTTATATGCAACCATTGATCTAGAGAAGGCCAGAGTTGGAAGAACAAGAATACTAGAGAAAGAACATTCTAACCCAAAATGGTATGAGACTTTCCATATCTACTGTGCTCATATGGCTTCCAACGTCATCTTCACTGTCAAGGATGACAATCCCATCGGAGCAACCTTAATTGGCAGAGCATATTTGTCTGTCCAAGACATCATACACGGGGAAGAAGTGGACAAATGGGTTCCAATCTTGGATGAACGCAAGAACCCCATTCGTGGGGAGCCCAAGATTCATGTCAAATTACAATATTTTGATGTCACAAAGGATCGAAACTGGGGTCGAGGCATCAAAACCCCCAAGTTTCCTGGTGTTCCCTACACTTACTTCTCTCAGAGACGAGGCTGTAAGGTAACTTTGTATCAAGATGCTCATGTTCCAGACAACTTTATTCCCAGAATCCCTCTTGCCGGAGGCAAGTTTTATGAGCCACACCGATGTTGGGAAGATGTTTTTGATGCAATAACTAATGCAAAACACTTGATTTACATTACTGGATGGTCTGTTTACACTGAGATTTCTTTGGTGAGAGATACAAGAAGACCAAAGCCTGGTGGAGACATCATACTCGGCGAATTGCTTAAGAGAAAGGCAAGTGAAGGTGTTAGAGTTCTTTTACTTGTTTGGGATGATAAGACTTCGGTGCCGGTGTTGAAGAAGGATGGGCTGATGGCAACTCATGATGAAGATACTGCAAAGTACTTTGAGAATACTGATGTGCACTGTGTGTTGTGCCCTCGCAATCCTGATGATGGTGCGAACGTTATTCAGGACATAGCTGTTGGGACGATGTTCACTCATCACCAGAAGATTGTTGTGGTGGACAGCGCCTTGCCTAGTGGGGATCCAAACAGAAGGAGAATAGTGAGTTTTGTTGGTGGGATTGATCTCTGTGATGGCAGGTACGATACCCCATTTCACTCCCTTTTCAGGACGTTGGATTCTGCTCATCATGATGATTTCCATCAGCCAAACTTCAATGGTACTTCCATAAACAAAGGTGGTCCGAGAGAGCCGTGGCACGACATCCATTCCCGGCTCGAAGGACCTGTCGCATGGGATGTTATGTACAACTTTGAACAGAGATGGAAGAAGCAAGGTGGGAAGGATTTGCTTGTTAAACTGAGAGATCTAGATGACATTTTCATCACCCCATCGCCTGTGATGTTCCCAGATGACTTTGACAGTTGGAATGTTCAAGTTTTCCGGTCGATCGATGGCGGGGCGGCTTTCGGCTTCCCTGAGACGCCTGAAGATGCAGCCAAAGCTGGGCTTATAAGTGGAAAGGATAACATTATTGATCGAAGCATTCAGGATGCTTACATTCATGCTATCCGACGGGCAAAGAATTTCATATACATTGAAAACCAGTATTTCCTTGGAAGCTCGTTTGAGTGGAGAGGGGATGATGTGAAACCTGAGATGATCAATGCCTTGCATTTAATCCCAAAGGAACTTTCACTTAAGATTGTGAGCAAGATTGAAGCTAGAGAGAGGTTCACCGTGTACATTGTCGTCCCGATGTGGCCAGAAGGGCTGCCGGAGGGGGAACAGTTCAGGCGATATTAG

Coding sequence (CDS)

ATGGCTCATATTCTGCTCCATGGCACACTTCATGTCACCATTTATGAAGTGGATAGGCTGCATTCCAGTGGCCGTAATATCTTCAAGAAGTTGGTTGAAAATGTAGAAGAAGCAGTTGGGTTTGGCCGAGGAGTAACTAAGTTATATGCAACCATTGATCTAGAGAAGGCCAGAGTTGGAAGAACAAGAATACTAGAGAAAGAACATTCTAACCCAAAATGGTATGAGACTTTCCATATCTACTGTGCTCATATGGCTTCCAACGTCATCTTCACTGTCAAGGATGACAATCCCATCGGAGCAACCTTAATTGGCAGAGCATATTTGTCTGTCCAAGACATCATACACGGGGAAGAAGTGGACAAATGGGTTCCAATCTTGGATGAACGCAAGAACCCCATTCGTGGGGAGCCCAAGATTCATGTCAAATTACAATATTTTGATGTCACAAAGGATCGAAACTGGGGTCGAGGCATCAAAACCCCCAAGTTTCCTGGTGTTCCCTACACTTACTTCTCTCAGAGACGAGGCTGTAAGGTAACTTTGTATCAAGATGCTCATGTTCCAGACAACTTTATTCCCAGAATCCCTCTTGCCGGAGGCAAGTTTTATGAGCCACACCGATGTTGGGAAGATGTTTTTGATGCAATAACTAATGCAAAACACTTGATTTACATTACTGGATGGTCTGTTTACACTGAGATTTCTTTGGTGAGAGATACAAGAAGACCAAAGCCTGGTGGAGACATCATACTCGGCGAATTGCTTAAGAGAAAGGCAAGTGAAGGTGTTAGAGTTCTTTTACTTGTTTGGGATGATAAGACTTCGGTGCCGGTGTTGAAGAAGGATGGGCTGATGGCAACTCATGATGAAGATACTGCAAAGTACTTTGAGAATACTGATGTGCACTGTGTGTTGTGCCCTCGCAATCCTGATGATGGTGCGAACGTTATTCAGGACATAGCTGTTGGGACGATGTTCACTCATCACCAGAAGATTGTTGTGGTGGACAGCGCCTTGCCTAGTGGGGATCCAAACAGAAGGAGAATAGTGAGTTTTGTTGGTGGGATTGATCTCTGTGATGGCAGGTACGATACCCCATTTCACTCCCTTTTCAGGACGTTGGATTCTGCTCATCATGATGATTTCCATCAGCCAAACTTCAATGGTACTTCCATAAACAAAGGTGGTCCGAGAGAGCCGTGGCACGACATCCATTCCCGGCTCGAAGGACCTGTCGCATGGGATGTTATGTACAACTTTGAACAGAGATGGAAGAAGCAAGGTGGGAAGGATTTGCTTGTTAAACTGAGAGATCTAGATGACATTTTCATCACCCCATCGCCTGTGATGTTCCCAGATGACTTTGACAGTTGGAATGTTCAAGTTTTCCGGTCGATCGATGGCGGGGCGGCTTTCGGCTTCCCTGAGACGCCTGAAGATGCAGCCAAAGCTGGGCTTATAAGTGGAAAGGATAACATTATTGATCGAAGCATTCAGGATGCTTACATTCATGCTATCCGACGGGCAAAGAATTTCATATACATTGAAAACCAGTATTTCCTTGGAAGCTCGTTTGAGTGGAGAGGGGATGATGTGAAACCTGAGATGATCAATGCCTTGCATTTAATCCCAAAGGAACTTTCACTTAAGATTGTGAGCAAGATTGAAGCTAGAGAGAGGTTCACCGTGTACATTGTCGTCCCGATGTGGCCAGAAGGGCTGCCGGAGGGGGAACAGTTCAGGCGATATTAG

Protein sequence

MAHILLHGTLHVTIYEVDRLHSSGRNIFKKLVENVEEAVGFGRGVTKLYATIDLEKARVGRTRILEKEHSNPKWYETFHIYCAHMASNVIFTVKDDNPIGATLIGRAYLSVQDIIHGEEVDKWVPILDERKNPIRGEPKIHVKLQYFDVTKDRNWGRGIKTPKFPGVPYTYFSQRRGCKVTLYQDAHVPDNFIPRIPLAGGKFYEPHRCWEDVFDAITNAKHLIYITGWSVYTEISLVRDTRRPKPGGDIILGELLKRKASEGVRVLLLVWDDKTSVPVLKKDGLMATHDEDTAKYFENTDVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPSGDPNRRRIVSFVGGIDLCDGRYDTPFHSLFRTLDSAHHDDFHQPNFNGTSINKGGPREPWHDIHSRLEGPVAWDVMYNFEQRWKKQGGKDLLVKLRDLDDIFITPSPVMFPDDFDSWNVQVFRSIDGGAAFGFPETPEDAAKAGLISGKDNIIDRSIQDAYIHAIRRAKNFIYIENQYFLGSSFEWRGDDVKPEMINALHLIPKELSLKIVSKIEARERFTVYIVVPMWPEGLPEGEQFRRY
Homology
BLAST of Sgr015778 vs. NCBI nr
Match: XP_038880369.1 (phospholipase D alpha 1 [Benincasa hispida] >XP_038880372.1 phospholipase D alpha 1 [Benincasa hispida])

HSP 1 Score: 1159.4 bits (2998), Expect = 0.0e+00
Identity = 540/578 (93.43%), Postives = 564/578 (97.58%), Query Frame = 0

Query: 1   MAHILLHGTLHVTIYEVDRLHSSGRNIFKKLVENVEEAVGFGRGVTKLYATIDLEKARVG 60
           MAHILLHGTLHVTIYEVD+LHS GRN  K+LVENVEEAVGFGRGVT+LYATIDLEKARVG
Sbjct: 1   MAHILLHGTLHVTIYEVDKLHSGGRNFLKQLVENVEEAVGFGRGVTRLYATIDLEKARVG 60

Query: 61  RTRILEKEHSNPKWYETFHIYCAHMASNVIFTVKDDNPIGATLIGRAYLSVQDIIHGEEV 120
           RTR LE+EHSNPKWYETFHIYCAHMASN+IFTVKDDNPIGATLIGRAYL V++IIHGEEV
Sbjct: 61  RTRRLEREHSNPKWYETFHIYCAHMASNIIFTVKDDNPIGATLIGRAYLPVREIIHGEEV 120

Query: 121 DKWVPILDERKNPIRGEPKIHVKLQYFDVTKDRNWGRGIKTPKFPGVPYTYFSQRRGCKV 180
           DKWVPILDERKNPIRGEPKIHVKLQYFDVTKD+NWGRGIK+PKFPGVPYTYFSQRRGCKV
Sbjct: 121 DKWVPILDERKNPIRGEPKIHVKLQYFDVTKDQNWGRGIKSPKFPGVPYTYFSQRRGCKV 180

Query: 181 TLYQDAHVPDNFIPRIPLAGGKFYEPHRCWEDVFDAITNAKHLIYITGWSVYTEISLVRD 240
           TLYQDAHVPDNFIP+IPLAGGKFYEPHRCWEDVFDAITNAKHLIYITGWSVYTEISLVRD
Sbjct: 181 TLYQDAHVPDNFIPKIPLAGGKFYEPHRCWEDVFDAITNAKHLIYITGWSVYTEISLVRD 240

Query: 241 TRRPKPGGDIILGELLKRKASEGVRVLLLVWDDKTSVPVLKKDGLMATHDEDTAKYFENT 300
            RR KPGGDI+LGELLKRKA EGVRVLLLVW+D TSVP+LK++GLMATHDEDTAKYF NT
Sbjct: 241 RRRSKPGGDIMLGELLKRKAGEGVRVLLLVWNDTTSVPILKEEGLMATHDEDTAKYFANT 300

Query: 301 DVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPSGDPNRRRIVSFVGGIDLC 360
           DVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPSGDPNRRRIVSFVGGIDLC
Sbjct: 301 DVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPSGDPNRRRIVSFVGGIDLC 360

Query: 361 DGRYDTPFHSLFRTLDSAHHDDFHQPNFNGTSINKGGPREPWHDIHSRLEGPVAWDVMYN 420
           DGRYDTPFHSLFRTLDSAHHDDFHQPNF GTSINKGGPREPWHDIHSRLEGPVAWDV+YN
Sbjct: 361 DGRYDTPFHSLFRTLDSAHHDDFHQPNFAGTSINKGGPREPWHDIHSRLEGPVAWDVLYN 420

Query: 421 FEQRWKKQGGKDLLVKLRDLDDIFITPSPVMFPDDFDSWNVQVFRSIDGGAAFGFPETPE 480
           FEQRW+KQGGKDLL+KLRDLD+IFITPSPV FPDDFDSWNVQVFRSIDGGAAFGFP++PE
Sbjct: 421 FEQRWRKQGGKDLLLKLRDLDEIFITPSPVTFPDDFDSWNVQVFRSIDGGAAFGFPDSPE 480

Query: 481 DAAKAGLISGKDNIIDRSIQDAYIHAIRRAKNFIYIENQYFLGSSFEWRGDDVKPEMINA 540
           +AAKAGL+SGKDN IDRSIQDAYI+AIRRAKNFIYIENQYFLGSSFEWRGDDVKPEMINA
Sbjct: 481 EAAKAGLVSGKDNTIDRSIQDAYINAIRRAKNFIYIENQYFLGSSFEWRGDDVKPEMINA 540

Query: 541 LHLIPKELSLKIVSKIEARERFTVYIVVPMWPEGLPEG 579
           LHLIPKELSLKIVSKIEARERFTVYIVVPMWPEG PEG
Sbjct: 541 LHLIPKELSLKIVSKIEARERFTVYIVVPMWPEGFPEG 578

BLAST of Sgr015778 vs. NCBI nr
Match: XP_022153673.1 (phospholipase D alpha 1-like [Momordica charantia])

HSP 1 Score: 1156.4 bits (2990), Expect = 0.0e+00
Identity = 538/578 (93.08%), Postives = 564/578 (97.58%), Query Frame = 0

Query: 1   MAHILLHGTLHVTIYEVDRLHSSGRNIFKKLVENVEEAVGFGRGVTKLYATIDLEKARVG 60
           MAHILLHGTLHVTIYEVD+LHSS  NIF KL ENVEEA+GFGRG+TKLYATIDLEKARVG
Sbjct: 1   MAHILLHGTLHVTIYEVDKLHSS-HNIFTKLRENVEEAIGFGRGLTKLYATIDLEKARVG 60

Query: 61  RTRILEKEHSNPKWYETFHIYCAHMASNVIFTVKDDNPIGATLIGRAYLSVQDIIHGEEV 120
           RTRIL+KEHSNPKWYETFHIYCAH ASN+IFTVKDDNPIGATLIGRAYLSVQ+I+ G+EV
Sbjct: 61  RTRILQKEHSNPKWYETFHIYCAHTASNIIFTVKDDNPIGATLIGRAYLSVQEIVSGQEV 120

Query: 121 DKWVPILDERKNPIRGEPKIHVKLQYFDVTKDRNWGRGIKTPKFPGVPYTYFSQRRGCKV 180
           D WVPILDE KNPIRG+PKIHVKLQYFDVTKDRNWGRGIK+PKFPGVPYTYFSQRRGCKV
Sbjct: 121 DTWVPILDESKNPIRGDPKIHVKLQYFDVTKDRNWGRGIKSPKFPGVPYTYFSQRRGCKV 180

Query: 181 TLYQDAHVPDNFIPRIPLAGGKFYEPHRCWEDVFDAITNAKHLIYITGWSVYTEISLVRD 240
           TLYQDAHVPDNF+PRIPLAGGKFYEPHRCWEDVFDAITNAKHLIYITGWSVYTEISLVRD
Sbjct: 181 TLYQDAHVPDNFVPRIPLAGGKFYEPHRCWEDVFDAITNAKHLIYITGWSVYTEISLVRD 240

Query: 241 TRRPKPGGDIILGELLKRKASEGVRVLLLVWDDKTSVPVLKKDGLMATHDEDTAKYFENT 300
           TRRPKPGGD++LGELLKRKASEGVRVLLLVWDDKTSVPVLK++GLMATHDEDTAKYFENT
Sbjct: 241 TRRPKPGGDVMLGELLKRKASEGVRVLLLVWDDKTSVPVLKEEGLMATHDEDTAKYFENT 300

Query: 301 DVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPSGDPNRRRIVSFVGGIDLC 360
           DVHCVLCPRNPDDGANVIQDIAVGTMFTHHQK VVVDS LP+GDPNRRRIVSFVGGIDLC
Sbjct: 301 DVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKTVVVDSGLPNGDPNRRRIVSFVGGIDLC 360

Query: 361 DGRYDTPFHSLFRTLDSAHHDDFHQPNFNGTSINKGGPREPWHDIHSRLEGPVAWDVMYN 420
           DGRYDTPFHSLFRTLDSAHHDDFHQPNF GTSINKGGPREPWHDIHSRLEGPVAWDV++N
Sbjct: 361 DGRYDTPFHSLFRTLDSAHHDDFHQPNFTGTSINKGGPREPWHDIHSRLEGPVAWDVLFN 420

Query: 421 FEQRWKKQGGKDLLVKLRDLDDIFITPSPVMFPDDFDSWNVQVFRSIDGGAAFGFPETPE 480
           FEQRW+KQGGKD+LVKLRDLDDIFITPSPV FPDDFDSWNVQVFRSIDGGAAFGFP++PE
Sbjct: 421 FEQRWRKQGGKDMLVKLRDLDDIFITPSPVTFPDDFDSWNVQVFRSIDGGAAFGFPDSPE 480

Query: 481 DAAKAGLISGKDNIIDRSIQDAYIHAIRRAKNFIYIENQYFLGSSFEWRGDDVKPEMINA 540
           +AAK+GLISGKDNIIDRSIQDAYIHAIRRAKNFIY+ENQYFLGSSFEWRG DVKPE INA
Sbjct: 481 EAAKSGLISGKDNIIDRSIQDAYIHAIRRAKNFIYVENQYFLGSSFEWRGSDVKPETINA 540

Query: 541 LHLIPKELSLKIVSKIEARERFTVYIVVPMWPEGLPEG 579
           LHLIPKELSLKIVSKIEARERF+VYIVVPMWPEGLPEG
Sbjct: 541 LHLIPKELSLKIVSKIEARERFSVYIVVPMWPEGLPEG 577

BLAST of Sgr015778 vs. NCBI nr
Match: KAG7036505.1 (Phospholipase D alpha 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1135.2 bits (2935), Expect = 0.0e+00
Identity = 527/578 (91.18%), Postives = 558/578 (96.54%), Query Frame = 0

Query: 1   MAHILLHGTLHVTIYEVDRLHSSGRNIFKKLVENVEEAVGFGRGVTKLYATIDLEKARVG 60
           MAHILLHGTLHVTIYEVD+LHS  RN  K LVENVEEAVGFGRGVT+LYATIDLEKARVG
Sbjct: 1   MAHILLHGTLHVTIYEVDKLHSGARNFLKSLVENVEEAVGFGRGVTRLYATIDLEKARVG 60

Query: 61  RTRILEKEHSNPKWYETFHIYCAHMASNVIFTVKDDNPIGATLIGRAYLSVQDIIHGEEV 120
           RTR LEKEHSNPKWYETFHIYCAHMASN+IFTVKDDNPIGATLIGRAYL V++II GEEV
Sbjct: 61  RTRRLEKEHSNPKWYETFHIYCAHMASNIIFTVKDDNPIGATLIGRAYLPVKEIISGEEV 120

Query: 121 DKWVPILDERKNPIRGEPKIHVKLQYFDVTKDRNWGRGIKTPKFPGVPYTYFSQRRGCKV 180
           D+WVPILDERK PIRG+PKIHVKLQYFDVTKD+NWGRGIK+ KFPGVPYTYFSQR+GCKV
Sbjct: 121 DQWVPILDERKKPIRGDPKIHVKLQYFDVTKDQNWGRGIKSSKFPGVPYTYFSQRQGCKV 180

Query: 181 TLYQDAHVPDNFIPRIPLAGGKFYEPHRCWEDVFDAITNAKHLIYITGWSVYTEISLVRD 240
           TLYQDAHVPDNFIP+IPLAGGKFYEP+RCWED+FDAI NAKH+IYITGWSVYTEISLVRD
Sbjct: 181 TLYQDAHVPDNFIPKIPLAGGKFYEPNRCWEDIFDAIINAKHMIYITGWSVYTEISLVRD 240

Query: 241 TRRPKPGGDIILGELLKRKASEGVRVLLLVWDDKTSVPVLKKDGLMATHDEDTAKYFENT 300
            RRPKPGGD++LGELLK+KA+EGVRVLLLVW+D TSVP+LK+DGLMATHDEDTAK+F NT
Sbjct: 241 KRRPKPGGDMMLGELLKKKANEGVRVLLLVWNDTTSVPILKEDGLMATHDEDTAKFFANT 300

Query: 301 DVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPSGDPNRRRIVSFVGGIDLC 360
           DVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALP+GDPNRRRIVSFVGGIDLC
Sbjct: 301 DVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPNGDPNRRRIVSFVGGIDLC 360

Query: 361 DGRYDTPFHSLFRTLDSAHHDDFHQPNFNGTSINKGGPREPWHDIHSRLEGPVAWDVMYN 420
           DGRYDTPFHSLFRTLDSAHHDDFHQPNF GTSINKGGPREPWHDIHSRLEGPVAWDV+YN
Sbjct: 361 DGRYDTPFHSLFRTLDSAHHDDFHQPNFTGTSINKGGPREPWHDIHSRLEGPVAWDVLYN 420

Query: 421 FEQRWKKQGGKDLLVKLRDLDDIFITPSPVMFPDDFDSWNVQVFRSIDGGAAFGFPETPE 480
           FEQRW+KQGGKDLLVKLR+LD IFI PSPVMFPDDFDSWN QVFRSIDGGAAFGFP++PE
Sbjct: 421 FEQRWRKQGGKDLLVKLRELDAIFIPPSPVMFPDDFDSWNAQVFRSIDGGAAFGFPDSPE 480

Query: 481 DAAKAGLISGKDNIIDRSIQDAYIHAIRRAKNFIYIENQYFLGSSFEWRGDDVKPEMINA 540
           +AAKAGLISGKDNIIDRSIQDAYI+AIRRAKNFIYIENQYFLGSSFEWRG DVKPEMINA
Sbjct: 481 EAAKAGLISGKDNIIDRSIQDAYINAIRRAKNFIYIENQYFLGSSFEWRGTDVKPEMINA 540

Query: 541 LHLIPKELSLKIVSKIEARERFTVYIVVPMWPEGLPEG 579
           LHLIPKELSLKIVSKIEARERFTVY+VVPMWPEG PEG
Sbjct: 541 LHLIPKELSLKIVSKIEARERFTVYVVVPMWPEGFPEG 578

BLAST of Sgr015778 vs. NCBI nr
Match: KAG6606795.1 (Phospholipase D alpha 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1135.2 bits (2935), Expect = 0.0e+00
Identity = 527/578 (91.18%), Postives = 558/578 (96.54%), Query Frame = 0

Query: 1   MAHILLHGTLHVTIYEVDRLHSSGRNIFKKLVENVEEAVGFGRGVTKLYATIDLEKARVG 60
           MAHILLHGTLHVTIYEVD+LHS  RN  K LVENVEEAVGFGRGVT+LYATIDLEKARVG
Sbjct: 1   MAHILLHGTLHVTIYEVDKLHSGARNFLKSLVENVEEAVGFGRGVTRLYATIDLEKARVG 60

Query: 61  RTRILEKEHSNPKWYETFHIYCAHMASNVIFTVKDDNPIGATLIGRAYLSVQDIIHGEEV 120
           RTR LEKEHSNPKWYETFHIYCAHMASN+IFTVKDDNPIGATLIGRAYL V++II GEEV
Sbjct: 61  RTRRLEKEHSNPKWYETFHIYCAHMASNIIFTVKDDNPIGATLIGRAYLPVKEIISGEEV 120

Query: 121 DKWVPILDERKNPIRGEPKIHVKLQYFDVTKDRNWGRGIKTPKFPGVPYTYFSQRRGCKV 180
           D+WVPILDERK PIRG+PKIHVKLQYFDVTKD+NWGRGIK+ KFPGVPYTYFSQR+GCKV
Sbjct: 121 DQWVPILDERKKPIRGDPKIHVKLQYFDVTKDQNWGRGIKSSKFPGVPYTYFSQRQGCKV 180

Query: 181 TLYQDAHVPDNFIPRIPLAGGKFYEPHRCWEDVFDAITNAKHLIYITGWSVYTEISLVRD 240
           TLYQDAHVPDNFIP+IPLAGGKFYEP+RCWED+FDAI NAKH+IYITGWSVYTEISLVRD
Sbjct: 181 TLYQDAHVPDNFIPKIPLAGGKFYEPNRCWEDIFDAIINAKHMIYITGWSVYTEISLVRD 240

Query: 241 TRRPKPGGDIILGELLKRKASEGVRVLLLVWDDKTSVPVLKKDGLMATHDEDTAKYFENT 300
            RRPKPGGD++LGELLK+KA+EGVRVLLLVW+D TSVP+LK+DGLMATHDEDTAK+F NT
Sbjct: 241 KRRPKPGGDMMLGELLKKKANEGVRVLLLVWNDTTSVPILKEDGLMATHDEDTAKFFANT 300

Query: 301 DVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPSGDPNRRRIVSFVGGIDLC 360
           DVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALP+GDPNRRRIVSFVGGIDLC
Sbjct: 301 DVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPNGDPNRRRIVSFVGGIDLC 360

Query: 361 DGRYDTPFHSLFRTLDSAHHDDFHQPNFNGTSINKGGPREPWHDIHSRLEGPVAWDVMYN 420
           DGRYDTPFHSLFRTLDSAHHDDFHQPNF GTSINKGGPREPWHDIHSRLEGPVAWDV+YN
Sbjct: 361 DGRYDTPFHSLFRTLDSAHHDDFHQPNFTGTSINKGGPREPWHDIHSRLEGPVAWDVLYN 420

Query: 421 FEQRWKKQGGKDLLVKLRDLDDIFITPSPVMFPDDFDSWNVQVFRSIDGGAAFGFPETPE 480
           FEQRW+KQGGKDLLVKLR+LD IFI PSPVMFPDDFDSWN QVFRSIDGGAAFGFP++PE
Sbjct: 421 FEQRWRKQGGKDLLVKLRELDAIFIPPSPVMFPDDFDSWNAQVFRSIDGGAAFGFPDSPE 480

Query: 481 DAAKAGLISGKDNIIDRSIQDAYIHAIRRAKNFIYIENQYFLGSSFEWRGDDVKPEMINA 540
           +AAKAGLISGKDNIIDRSIQDAYI+AIRRAKNFIYIENQYFLGSSFEWRG DVKPEMINA
Sbjct: 481 EAAKAGLISGKDNIIDRSIQDAYINAIRRAKNFIYIENQYFLGSSFEWRGTDVKPEMINA 540

Query: 541 LHLIPKELSLKIVSKIEARERFTVYIVVPMWPEGLPEG 579
           LHLIPKELSLKIVSKIEARERFTVY+VVPMWPEG PEG
Sbjct: 541 LHLIPKELSLKIVSKIEARERFTVYVVVPMWPEGFPEG 578

BLAST of Sgr015778 vs. NCBI nr
Match: XP_023523927.1 (phospholipase D alpha 1-like [Cucurbita pepo subsp. pepo] >XP_023523928.1 phospholipase D alpha 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1135.2 bits (2935), Expect = 0.0e+00
Identity = 527/578 (91.18%), Postives = 558/578 (96.54%), Query Frame = 0

Query: 1   MAHILLHGTLHVTIYEVDRLHSSGRNIFKKLVENVEEAVGFGRGVTKLYATIDLEKARVG 60
           MAHILLHGTLHVTIYEVD+LHS  RN  K LVENVEEAVGFGRGVT+LYATIDLEKARVG
Sbjct: 1   MAHILLHGTLHVTIYEVDKLHSGARNFLKSLVENVEEAVGFGRGVTRLYATIDLEKARVG 60

Query: 61  RTRILEKEHSNPKWYETFHIYCAHMASNVIFTVKDDNPIGATLIGRAYLSVQDIIHGEEV 120
           RTR LEKEHSNPKWYETFHIYCAHMASN+IFTVKDDNPIGATLIGRAYL V++II GEEV
Sbjct: 61  RTRRLEKEHSNPKWYETFHIYCAHMASNIIFTVKDDNPIGATLIGRAYLPVKEIISGEEV 120

Query: 121 DKWVPILDERKNPIRGEPKIHVKLQYFDVTKDRNWGRGIKTPKFPGVPYTYFSQRRGCKV 180
           D+WVPILDERK PIRG+PKIHVKLQYFDVTKD+NWGRGIK+ KFPGVPYTYFSQR+GCKV
Sbjct: 121 DQWVPILDERKKPIRGDPKIHVKLQYFDVTKDQNWGRGIKSSKFPGVPYTYFSQRQGCKV 180

Query: 181 TLYQDAHVPDNFIPRIPLAGGKFYEPHRCWEDVFDAITNAKHLIYITGWSVYTEISLVRD 240
           TLYQDAHVPDNFIP+IPLAGGKFYEP+RCWED+FDAI NAKH+IYITGWSVYTEISLVRD
Sbjct: 181 TLYQDAHVPDNFIPKIPLAGGKFYEPNRCWEDIFDAIINAKHMIYITGWSVYTEISLVRD 240

Query: 241 TRRPKPGGDIILGELLKRKASEGVRVLLLVWDDKTSVPVLKKDGLMATHDEDTAKYFENT 300
            RRPKPGGD++LGELLK+KA+EGVRVLLLVW+D TSVP+LK+DGLMATHDEDTAK+F NT
Sbjct: 241 KRRPKPGGDMMLGELLKKKANEGVRVLLLVWNDTTSVPILKEDGLMATHDEDTAKFFANT 300

Query: 301 DVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPSGDPNRRRIVSFVGGIDLC 360
           DVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALP+GDPNRRRIVSFVGGIDLC
Sbjct: 301 DVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPNGDPNRRRIVSFVGGIDLC 360

Query: 361 DGRYDTPFHSLFRTLDSAHHDDFHQPNFNGTSINKGGPREPWHDIHSRLEGPVAWDVMYN 420
           DGRYDTPFHSLFRTLDSAHHDDFHQPNF GTSINKGGPREPWHDIHSRLEGPVAWDV+YN
Sbjct: 361 DGRYDTPFHSLFRTLDSAHHDDFHQPNFTGTSINKGGPREPWHDIHSRLEGPVAWDVLYN 420

Query: 421 FEQRWKKQGGKDLLVKLRDLDDIFITPSPVMFPDDFDSWNVQVFRSIDGGAAFGFPETPE 480
           FEQRW+KQGGKDLLVKLR+LD IFI PSPVMFPDDFDSWN QVFRSIDGGAAFGFP++PE
Sbjct: 421 FEQRWRKQGGKDLLVKLRELDAIFIPPSPVMFPDDFDSWNAQVFRSIDGGAAFGFPDSPE 480

Query: 481 DAAKAGLISGKDNIIDRSIQDAYIHAIRRAKNFIYIENQYFLGSSFEWRGDDVKPEMINA 540
           +AAKAGLISGKDNIIDRSIQDAYI+AIRRAKNFIYIENQYFLGSSFEWRG DVKPEMINA
Sbjct: 481 EAAKAGLISGKDNIIDRSIQDAYINAIRRAKNFIYIENQYFLGSSFEWRGTDVKPEMINA 540

Query: 541 LHLIPKELSLKIVSKIEARERFTVYIVVPMWPEGLPEG 579
           LHLIPKELSLKIVSKIEARERFTVY+VVPMWPEG PEG
Sbjct: 541 LHLIPKELSLKIVSKIEARERFTVYVVVPMWPEGFPEG 578

BLAST of Sgr015778 vs. ExPASy Swiss-Prot
Match: Q41142 (Phospholipase D alpha 1 OS=Ricinus communis OX=3988 GN=PLD1 PE=1 SV=1)

HSP 1 Score: 1042.3 bits (2694), Expect = 2.0e-303
Identity = 471/578 (81.49%), Postives = 534/578 (92.39%), Query Frame = 0

Query: 1   MAHILLHGTLHVTIYEVDRLHS-SGRNIFKKLVENVEEAVGFGRGVTKLYATIDLEKARV 60
           MA I LHGTLHVTIYEVD+LHS  G + F+KLVEN+EE VGFG+GV+KLYATIDLEKARV
Sbjct: 1   MAQISLHGTLHVTIYEVDKLHSGGGPHFFRKLVENIEETVGFGKGVSKLYATIDLEKARV 60

Query: 61  GRTRILEKEHSNPKWYETFHIYCAHMASNVIFTVKDDNPIGATLIGRAYLSVQDIIHGEE 120
           GRTRILE E SNP+WYE+FH+YCAH ASNVIFTVKDDNPIGATLIGRAY+ V++++ GEE
Sbjct: 61  GRTRILENEQSNPRWYESFHVYCAHQASNVIFTVKDDNPIGATLIGRAYVPVEELLDGEE 120

Query: 121 VDKWVPILDERKNPIRGEPKIHVKLQYFDVTKDRNWGRGIKTPKFPGVPYTYFSQRRGCK 180
           +D+WV ILDE KNP+    KIHVKLQYF+VTKDRNWG+GI++ K+PGVPYTYFSQR+GCK
Sbjct: 121 IDRWVEILDEDKNPVHSGSKIHVKLQYFEVTKDRNWGQGIRSSKYPGVPYTYFSQRQGCK 180

Query: 181 VTLYQDAHVPDNFIPRIPLAGGKFYEPHRCWEDVFDAITNAKHLIYITGWSVYTEISLVR 240
           V+LYQDAH+PD F+P+IPLAGG +YEPHRCWEDVFDAITNAKHLIYITGWSVYTEISL+R
Sbjct: 181 VSLYQDAHIPDKFVPQIPLAGGNYYEPHRCWEDVFDAITNAKHLIYITGWSVYTEISLIR 240

Query: 241 DTRRPKPGGDIILGELLKRKASEGVRVLLLVWDDKTSVPVLKKDGLMATHDEDTAKYFEN 300
           D+RRPKPGGDI LGELLK+KASEGVRVL+LVWDD+TSV +LKKDGLMATHDE+T  +F+N
Sbjct: 241 DSRRPKPGGDITLGELLKKKASEGVRVLMLVWDDRTSVGLLKKDGLMATHDEETEHFFQN 300

Query: 301 TDVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPSGDPNRRRIVSFVGGIDL 360
           TDVHCVLCPRNPDDG + +QD+ + TMFTHHQKIVVVDSA+P+GD  RRRIVSFVGG+DL
Sbjct: 301 TDVHCVLCPRNPDDGGSFVQDLQISTMFTHHQKIVVVDSAMPNGDSQRRRIVSFVGGLDL 360

Query: 361 CDGRYDTPFHSLFRTLDSAHHDDFHQPNFNGTSINKGGPREPWHDIHSRLEGPVAWDVMY 420
           CDGRYD+PFHSLFRTLDSAHHDDFHQPNF G SI KGGPREPWHDIHSRLEGP+AWDV++
Sbjct: 361 CDGRYDSPFHSLFRTLDSAHHDDFHQPNFAGASIEKGGPREPWHDIHSRLEGPIAWDVLF 420

Query: 421 NFEQRWKKQGGKDLLVKLRDLDDIFITPSPVMFPDDFDSWNVQVFRSIDGGAAFGFPETP 480
           NFEQRW+KQGGKDLL++LR+L+D+ I PSPVM+PDDF++WNVQ+FRSIDGGAAFGFPETP
Sbjct: 421 NFEQRWRKQGGKDLLIQLRELEDVIIPPSPVMYPDDFEAWNVQLFRSIDGGAAFGFPETP 480

Query: 481 EDAAKAGLISGKDNIIDRSIQDAYIHAIRRAKNFIYIENQYFLGSSFEWRGDDVKPEMIN 540
           EDA +AGL+SGKDNIIDRSIQDAYIHAIRRAKNFIYIENQYFLGSSF W  D +KPE IN
Sbjct: 481 EDAPEAGLVSGKDNIIDRSIQDAYIHAIRRAKNFIYIENQYFLGSSFGWSPDGIKPEDIN 540

Query: 541 ALHLIPKELSLKIVSKIEARERFTVYIVVPMWPEGLPE 578
           ALHLIPKELSLKI+SKI A ERFTVYIVVPMWPEG+PE
Sbjct: 541 ALHLIPKELSLKILSKIAAGERFTVYIVVPMWPEGIPE 578

BLAST of Sgr015778 vs. ExPASy Swiss-Prot
Match: P86387 (Phospholipase D alpha 1 OS=Carica papaya OX=3649 GN=PLD1 PE=1 SV=1)

HSP 1 Score: 1010.0 bits (2610), Expect = 1.1e-293
Identity = 456/578 (78.89%), Postives = 525/578 (90.83%), Query Frame = 0

Query: 1   MAHILLHGTLHVTIYEVDRLHSSG-RNIFKKLVENVEEAVGFGRGVTKLYATIDLEKARV 60
           MAH L+HGTLH T+YEVD+LHS G    F K++ NVE  +G G+GVT+LYATIDLE+ARV
Sbjct: 1   MAHYLMHGTLHATVYEVDKLHSGGISGFFGKILANVEGTIGIGKGVTQLYATIDLERARV 60

Query: 61  GRTRILEKEHSNPKWYETFHIYCAHMASNVIFTVKDDNPIGATLIGRAYLSVQDIIHGEE 120
           GRTRI++ E +NPKWYE+FHIYCAHMASNV+FTVKDDNPIGATLIGRAY+ V+++I G++
Sbjct: 61  GRTRIIKDEPNNPKWYESFHIYCAHMASNVVFTVKDDNPIGATLIGRAYVPVEELIRGDQ 120

Query: 121 VDKWVPILDERKNPIRGEPKIHVKLQYFDVTKDRNWGRGIKTPKFPGVPYTYFSQRRGCK 180
           VD+WV ILDE KNPI G+ KIHVKLQ+FDV KD NW  GIK  ++ GVPYT++SQRRGC+
Sbjct: 121 VDRWVEILDEDKNPIEGDSKIHVKLQFFDVKKDSNWNMGIKGARYLGVPYTFYSQRRGCR 180

Query: 181 VTLYQDAHVPDNFIPRIPLAGGKFYEPHRCWEDVFDAITNAKHLIYITGWSVYTEISLVR 240
           V+LYQDAHVPD FIP+IPLAGGK+YEPHRCWEDVFDAITNA+HLIYITGWSVYTEI+L+R
Sbjct: 181 VSLYQDAHVPDGFIPKIPLAGGKYYEPHRCWEDVFDAITNARHLIYITGWSVYTEITLIR 240

Query: 241 DTRRPKPGGDIILGELLKRKASEGVRVLLLVWDDKTSVPVLKKDGLMATHDEDTAKYFEN 300
           D+RRPKPGGD+ LGELLK+KASEGV+VL+LVWDD+TSV +LKKDGLMATHDE+TA YF+N
Sbjct: 241 DSRRPKPGGDVTLGELLKQKASEGVKVLMLVWDDRTSVGLLKKDGLMATHDEETANYFQN 300

Query: 301 TDVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPSGDPNRRRIVSFVGGIDL 360
           TDVHCVLCPRNPDDG + +Q + + TMFTHHQKIVVVD  +PSG+   RRIVSFVGGIDL
Sbjct: 301 TDVHCVLCPRNPDDGGSFVQGLQISTMFTHHQKIVVVDGEMPSGESQMRRIVSFVGGIDL 360

Query: 361 CDGRYDTPFHSLFRTLDSAHHDDFHQPNFNGTSINKGGPREPWHDIHSRLEGPVAWDVMY 420
           CDGRYDTPFHSLFRTLD+AHHDDFHQPNF G+SI KGGPREPWHDIHSRLEGPVAWDV++
Sbjct: 361 CDGRYDTPFHSLFRTLDTAHHDDFHQPNFAGSSITKGGPREPWHDIHSRLEGPVAWDVLF 420

Query: 421 NFEQRWKKQGGKDLLVKLRDLDDIFITPSPVMFPDDFDSWNVQVFRSIDGGAAFGFPETP 480
           NFEQRW++QGGKD+LV LR+LD+I I PSPVMFPDD ++WNVQ+FRSIDGGAAFGFPETP
Sbjct: 421 NFEQRWRQQGGKDVLVNLRELDNIIIPPSPVMFPDDHETWNVQLFRSIDGGAAFGFPETP 480

Query: 481 EDAAKAGLISGKDNIIDRSIQDAYIHAIRRAKNFIYIENQYFLGSSFEWRGDDVKPEMIN 540
           E+AA+AGL+SGKDNIIDRSIQDAYI+AIRRAKNFIYIENQYFLGSSF+W  DD+K E IN
Sbjct: 481 EEAARAGLVSGKDNIIDRSIQDAYINAIRRAKNFIYIENQYFLGSSFDWSSDDIKREDIN 540

Query: 541 ALHLIPKELSLKIVSKIEARERFTVYIVVPMWPEGLPE 578
           ALHLIPKELSLKIVSKIE  ERFTVY+VVPMWPEG+PE
Sbjct: 541 ALHLIPKELSLKIVSKIERGERFTVYVVVPMWPEGVPE 578

BLAST of Sgr015778 vs. ExPASy Swiss-Prot
Match: O04865 (Phospholipase D alpha 1 OS=Vigna unguiculata OX=3917 GN=PLD1 PE=2 SV=1)

HSP 1 Score: 1005.4 bits (2598), Expect = 2.7e-292
Identity = 457/579 (78.93%), Postives = 517/579 (89.29%), Query Frame = 0

Query: 1   MAHILLHGTLHVTIYEVDRLH-SSGRNIFKKLVENVEEAVGFGRGVTKLYATIDLEKARV 60
           MA ILLHGTLH TIYEVD LH   G N F KL +N+EE VG G+GVTKLYATIDLEKARV
Sbjct: 1   MAQILLHGTLHATIYEVDELHGGGGGNFFSKLKQNIEETVGIGKGVTKLYATIDLEKARV 60

Query: 61  GRTRILEKEHSNPKWYETFHIYCAHMASNVIFTVKDDNPIGATLIGRAYLSVQDIIHGEE 120
           GRTRI+E E +NPKW E+FHIYC H+ASN+IFTVKDDNPIGATLIGRAY+ V +++ G E
Sbjct: 61  GRTRIIENETTNPKWNESFHIYCGHLASNIIFTVKDDNPIGATLIGRAYVPVSEVLDGHE 120

Query: 121 VDKWVPILDERKNPIRGEPKIHVKLQYFDVTKDRNWGRGIKTPKFPGVPYTYFSQRRGCK 180
           +DKWV ILD  KNPI G  KIHV+LQYFDV KDRNW RGI++PK+PGVPYT+FSQR+GCK
Sbjct: 121 IDKWVEILDTEKNPIEGGSKIHVRLQYFDVLKDRNWARGIRSPKYPGVPYTFFSQRQGCK 180

Query: 181 VTLYQDAHVPDNFIPRIPLAGGKFYEPHRCWEDVFDAITNAKHLIYITGWSVYTEISLVR 240
           V LYQDAHVPDNF+P+IPLAGGK YE HRCWED+FDAITNAKHLIYITGWSVYTEISL+R
Sbjct: 181 VFLYQDAHVPDNFVPKIPLAGGKNYEAHRCWEDIFDAITNAKHLIYITGWSVYTEISLIR 240

Query: 241 DTRRPKPGGDIILGELLKRKASEGVRVLLLVWDDKTSVPVLKKDGLMATHDEDTAKYFEN 300
           D+RRPK GGD  +GELLK+KASEGVRVL+LVWDD+TSV +LKKDGLMATHDE+T ++F +
Sbjct: 241 DSRRPKAGGDQTIGELLKKKASEGVRVLMLVWDDRTSVGLLKKDGLMATHDEETEQFFRD 300

Query: 301 TDVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALP-SGDPNRRRIVSFVGGID 360
           TDVHCVLCPRNPDDG +++QD+ + TMFTHHQKIVVVDSALP  G  ++RRIVSFVGG+D
Sbjct: 301 TDVHCVLCPRNPDDGGSIVQDLQISTMFTHHQKIVVVDSALPGGGGSDKRRIVSFVGGLD 360

Query: 361 LCDGRYDTPFHSLFRTLDSAHHDDFHQPNFNGTSINKGGPREPWHDIHSRLEGPVAWDVM 420
           LCDGRYDT FHSLFRTLD+AHHDDFHQPNF G +I KGGPREPWHDIHSR+EGP+AWDV+
Sbjct: 361 LCDGRYDTAFHSLFRTLDTAHHDDFHQPNFPGAAITKGGPREPWHDIHSRVEGPIAWDVL 420

Query: 421 YNFEQRWKKQGGKDLLVKLRDLDDIFITPSPVMFPDDFDSWNVQVFRSIDGGAAFGFPET 480
           +NFEQRW+KQGGKD+L  LR+L+D+ I PSPV FPDD ++WNVQ+FRSIDGGAAFGFP+T
Sbjct: 421 FNFEQRWRKQGGKDILAPLRELEDVIIPPSPVTFPDDHETWNVQLFRSIDGGAAFGFPDT 480

Query: 481 PEDAAKAGLISGKDNIIDRSIQDAYIHAIRRAKNFIYIENQYFLGSSFEWRGDDVKPEMI 540
           PEDAAKAGL+SGKDNIIDRSIQDAYIHAIRRAKNFIYIENQYFLGSSF W  DD+K E I
Sbjct: 481 PEDAAKAGLVSGKDNIIDRSIQDAYIHAIRRAKNFIYIENQYFLGSSFSWNNDDIKREEI 540

Query: 541 NALHLIPKELSLKIVSKIEARERFTVYIVVPMWPEGLPE 578
            ALHLIPKELSLKIVSKIEA ERF VY+VVPMWPEG+PE
Sbjct: 541 GALHLIPKELSLKIVSKIEAGERFAVYVVVPMWPEGIPE 579

BLAST of Sgr015778 vs. ExPASy Swiss-Prot
Match: P93400 (Phospholipase D alpha 1 OS=Nicotiana tabacum OX=4097 GN=PLD1 PE=2 SV=2)

HSP 1 Score: 981.1 bits (2535), Expect = 5.5e-285
Identity = 443/578 (76.64%), Postives = 517/578 (89.45%), Query Frame = 0

Query: 1   MAHILLHGTLHVTIYEVDRLH-SSGRNIFKKLVENVEEAVGFGRGVTKLYATIDLEKARV 60
           MA ILLHGTLHVTIYEVD L    G + F K+ E+VEE +GFG+G   +YAT+DLEKARV
Sbjct: 1   MAQILLHGTLHVTIYEVDNLQKEGGGHFFSKIKEHVEETIGFGKGTPAIYATVDLEKARV 60

Query: 61  GRTRILEKEHSNPKWYETFHIYCAHMASNVIFTVKDDNPIGATLIGRAYLSVQDIIHGEE 120
           GRTR ++ E +NP+WYE+FHIYCAHMASNVIFTVKDDNPIGATLIGRAY+ V++++ GEE
Sbjct: 61  GRTRKIKNEPNNPRWYESFHIYCAHMASNVIFTVKDDNPIGATLIGRAYVPVEELLEGEE 120

Query: 121 VDKWVPILDERKNPIRGEPKIHVKLQYFDVTKDRNWGRGIKTPKFPGVPYTYFSQRRGCK 180
           +DKWV ILD   NPI    KIHVKLQ+FDV++D NW RGI++ K+PGVPYT+F+QR GC+
Sbjct: 121 IDKWVEILDREMNPIAEGSKIHVKLQFFDVSRDPNWERGIRSSKYPGVPYTFFAQRTGCR 180

Query: 181 VTLYQDAHVPDNFIPRIPLAGGKFYEPHRCWEDVFDAITNAKHLIYITGWSVYTEISLVR 240
           V+LYQDAHVPDNFIP+IPL+GGK+YEPHRCWED+FDAI NAKHLIYITGWSVYTEI+LVR
Sbjct: 181 VSLYQDAHVPDNFIPKIPLSGGKYYEPHRCWEDIFDAIINAKHLIYITGWSVYTEITLVR 240

Query: 241 DTRRPKPGGDIILGELLKRKASEGVRVLLLVWDDKTSVPVLKKDGLMATHDEDTAKYFEN 300
           D+RR KPGGDI LGELLK+KASEGV+VL+LVWDD+TSV +LKKDGLMATHD++T ++F+ 
Sbjct: 241 DSRRQKPGGDITLGELLKKKASEGVKVLMLVWDDRTSVGLLKKDGLMATHDQETEQFFQG 300

Query: 301 TDVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPSGDPNRRRIVSFVGGIDL 360
           T+V+CVLCPRNPDDG +++Q + +GTMFTHHQKIVVVDS LPSG+  +RRI+SFVGGIDL
Sbjct: 301 TEVNCVLCPRNPDDGGSIVQSLQIGTMFTHHQKIVVVDSELPSGESEKRRILSFVGGIDL 360

Query: 361 CDGRYDTPFHSLFRTLDSAHHDDFHQPNFNGTSINKGGPREPWHDIHSRLEGPVAWDVMY 420
           CDGRYDTPFHSLFRTLD+AHHDDFHQPNF   +I KGGPREPWHDIHSRLEGP+AWDV++
Sbjct: 361 CDGRYDTPFHSLFRTLDTAHHDDFHQPNFPDGAITKGGPREPWHDIHSRLEGPIAWDVLF 420

Query: 421 NFEQRWKKQGGKDLLVKLRDLDDIFITPSPVMFPDDFDSWNVQVFRSIDGGAAFGFPETP 480
           NFEQRW+KQGGKD+LV  R+LDDI I PSPVM  DD ++WNVQ+FRSID GAAFGFPETP
Sbjct: 421 NFEQRWRKQGGKDVLVNFRELDDIIIPPSPVMHLDDSETWNVQLFRSIDEGAAFGFPETP 480

Query: 481 EDAAKAGLISGKDNIIDRSIQDAYIHAIRRAKNFIYIENQYFLGSSFEWRGDDVKPEMIN 540
           EDAAKAGL+SG DNIIDRSIQDAYIHAIRRAKNFIYIENQYFLGSS++W+ DD+K E I 
Sbjct: 481 EDAAKAGLVSGXDNIIDRSIQDAYIHAIRRAKNFIYIENQYFLGSSYDWQSDDIKVEDIG 540

Query: 541 ALHLIPKELSLKIVSKIEARERFTVYIVVPMWPEGLPE 578
           ALH+IPKEL+LKIVSKIEA ERFTVY+VVPMWPEG+PE
Sbjct: 541 ALHVIPKELALKIVSKIEAGERFTVYVVVPMWPEGIPE 578

BLAST of Sgr015778 vs. ExPASy Swiss-Prot
Match: Q38882 (Phospholipase D alpha 1 OS=Arabidopsis thaliana OX=3702 GN=PLDALPHA1 PE=1 SV=2)

HSP 1 Score: 980.3 bits (2533), Expect = 9.3e-285
Identity = 449/580 (77.41%), Postives = 507/580 (87.41%), Query Frame = 0

Query: 1   MAHILLHGTLHVTIYEVDRLHSSG--RNIFKKLVENVEEAVGFGRGVTKLYATIDLEKAR 60
           MA  LLHGTLH TIYEVD LH  G  +    K++ NVEE +G G+G T+LYATIDL+KAR
Sbjct: 1   MAQHLLHGTLHATIYEVDALHGGGVRQGFLGKILANVEETIGVGKGETQLYATIDLQKAR 60

Query: 61  VGRTRILEKEHSNPKWYETFHIYCAHMASNVIFTVKDDNPIGATLIGRAYLSVQDIIHGE 120
           VGRTR ++ E  NPKWYE+FHIYCAH+AS++IFTVKDDNPIGATLIGRAY+ V  +I+GE
Sbjct: 61  VGRTRKIKNEPKNPKWYESFHIYCAHLASDIIFTVKDDNPIGATLIGRAYIPVDQVINGE 120

Query: 121 EVDKWVPILDERKNPIRGEPKIHVKLQYFDVTKDRNWGRGIKTPKFPGVPYTYFSQRRGC 180
           EVD+WV ILD  +NPI+G  KIHVKLQYF V +DRNW  GIK+ KFPGVPYT+FSQR+GC
Sbjct: 121 EVDQWVEILDNDRNPIQGGSKIHVKLQYFHVEEDRNWNMGIKSAKFPGVPYTFFSQRQGC 180

Query: 181 KVTLYQDAHVPDNFIPRIPLAGGKFYEPHRCWEDVFDAITNAKHLIYITGWSVYTEISLV 240
           KV+LYQDAH+PDNF+PRIPLAGGK YEP RCWED+FDAI+NAKHLIYITGWSVY EI+LV
Sbjct: 181 KVSLYQDAHIPDNFVPRIPLAGGKNYEPQRCWEDIFDAISNAKHLIYITGWSVYAEIALV 240

Query: 241 RDTRRPKPGGDIILGELLKRKASEGVRVLLLVWDDKTSVPVLKKDGLMATHDEDTAKYFE 300
           RD+RRPKPGGD+ +GELLK+KASEGVRVLLLVWDD+TSV VLKKDGLMATHDE+T  +F 
Sbjct: 241 RDSRRPKPGGDVTIGELLKKKASEGVRVLLLVWDDRTSVDVLKKDGLMATHDEETENFFR 300

Query: 301 NTDVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPS-GDPNRRRIVSFVGGI 360
            +DVHC+LCPRNPDDG +++Q + + TMFTHHQKIVVVDS +PS G    RRIVSFVGGI
Sbjct: 301 GSDVHCILCPRNPDDGGSIVQSLQISTMFTHHQKIVVVDSEMPSRGGSEMRRIVSFVGGI 360

Query: 361 DLCDGRYDTPFHSLFRTLDSAHHDDFHQPNFNGTSINKGGPREPWHDIHSRLEGPVAWDV 420
           DLCDGRYDTPFHSLFRTLD+ HHDDFHQPNF G +I KGGPREPWHDIHSRLEGP+AWDV
Sbjct: 361 DLCDGRYDTPFHSLFRTLDTVHHDDFHQPNFTGAAITKGGPREPWHDIHSRLEGPIAWDV 420

Query: 421 MYNFEQRWKKQGGKDLLVKLRDLDDIFITPSPVMFPDDFDSWNVQVFRSIDGGAAFGFPE 480
           MYNFEQRW KQGGKD+LVKLRDL DI ITPSPVMF +D D WNVQ+FRSIDGGAA GFPE
Sbjct: 421 MYNFEQRWSKQGGKDILVKLRDLSDIIITPSPVMFQEDHDVWNVQLFRSIDGGAAAGFPE 480

Query: 481 TPEDAAKAGLISGKDNIIDRSIQDAYIHAIRRAKNFIYIENQYFLGSSFEWRGDDVKPEM 540
           +PE AA+AGL+SGKDNIIDRSIQDAYIHAIRRAK+FIY+ENQYFLGSSF W  D + PE 
Sbjct: 481 SPEAAAEAGLVSGKDNIIDRSIQDAYIHAIRRAKDFIYVENQYFLGSSFAWAADGITPED 540

Query: 541 INALHLIPKELSLKIVSKIEARERFTVYIVVPMWPEGLPE 578
           INALHLIPKELSLKIVSKIE  E+F VY+VVPMWPEGLPE
Sbjct: 541 INALHLIPKELSLKIVSKIEKGEKFRVYVVVPMWPEGLPE 580

BLAST of Sgr015778 vs. ExPASy TrEMBL
Match: A0A6J1DI41 (Phospholipase D OS=Momordica charantia OX=3673 GN=LOC111021129 PE=3 SV=1)

HSP 1 Score: 1156.4 bits (2990), Expect = 0.0e+00
Identity = 538/578 (93.08%), Postives = 564/578 (97.58%), Query Frame = 0

Query: 1   MAHILLHGTLHVTIYEVDRLHSSGRNIFKKLVENVEEAVGFGRGVTKLYATIDLEKARVG 60
           MAHILLHGTLHVTIYEVD+LHSS  NIF KL ENVEEA+GFGRG+TKLYATIDLEKARVG
Sbjct: 1   MAHILLHGTLHVTIYEVDKLHSS-HNIFTKLRENVEEAIGFGRGLTKLYATIDLEKARVG 60

Query: 61  RTRILEKEHSNPKWYETFHIYCAHMASNVIFTVKDDNPIGATLIGRAYLSVQDIIHGEEV 120
           RTRIL+KEHSNPKWYETFHIYCAH ASN+IFTVKDDNPIGATLIGRAYLSVQ+I+ G+EV
Sbjct: 61  RTRILQKEHSNPKWYETFHIYCAHTASNIIFTVKDDNPIGATLIGRAYLSVQEIVSGQEV 120

Query: 121 DKWVPILDERKNPIRGEPKIHVKLQYFDVTKDRNWGRGIKTPKFPGVPYTYFSQRRGCKV 180
           D WVPILDE KNPIRG+PKIHVKLQYFDVTKDRNWGRGIK+PKFPGVPYTYFSQRRGCKV
Sbjct: 121 DTWVPILDESKNPIRGDPKIHVKLQYFDVTKDRNWGRGIKSPKFPGVPYTYFSQRRGCKV 180

Query: 181 TLYQDAHVPDNFIPRIPLAGGKFYEPHRCWEDVFDAITNAKHLIYITGWSVYTEISLVRD 240
           TLYQDAHVPDNF+PRIPLAGGKFYEPHRCWEDVFDAITNAKHLIYITGWSVYTEISLVRD
Sbjct: 181 TLYQDAHVPDNFVPRIPLAGGKFYEPHRCWEDVFDAITNAKHLIYITGWSVYTEISLVRD 240

Query: 241 TRRPKPGGDIILGELLKRKASEGVRVLLLVWDDKTSVPVLKKDGLMATHDEDTAKYFENT 300
           TRRPKPGGD++LGELLKRKASEGVRVLLLVWDDKTSVPVLK++GLMATHDEDTAKYFENT
Sbjct: 241 TRRPKPGGDVMLGELLKRKASEGVRVLLLVWDDKTSVPVLKEEGLMATHDEDTAKYFENT 300

Query: 301 DVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPSGDPNRRRIVSFVGGIDLC 360
           DVHCVLCPRNPDDGANVIQDIAVGTMFTHHQK VVVDS LP+GDPNRRRIVSFVGGIDLC
Sbjct: 301 DVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKTVVVDSGLPNGDPNRRRIVSFVGGIDLC 360

Query: 361 DGRYDTPFHSLFRTLDSAHHDDFHQPNFNGTSINKGGPREPWHDIHSRLEGPVAWDVMYN 420
           DGRYDTPFHSLFRTLDSAHHDDFHQPNF GTSINKGGPREPWHDIHSRLEGPVAWDV++N
Sbjct: 361 DGRYDTPFHSLFRTLDSAHHDDFHQPNFTGTSINKGGPREPWHDIHSRLEGPVAWDVLFN 420

Query: 421 FEQRWKKQGGKDLLVKLRDLDDIFITPSPVMFPDDFDSWNVQVFRSIDGGAAFGFPETPE 480
           FEQRW+KQGGKD+LVKLRDLDDIFITPSPV FPDDFDSWNVQVFRSIDGGAAFGFP++PE
Sbjct: 421 FEQRWRKQGGKDMLVKLRDLDDIFITPSPVTFPDDFDSWNVQVFRSIDGGAAFGFPDSPE 480

Query: 481 DAAKAGLISGKDNIIDRSIQDAYIHAIRRAKNFIYIENQYFLGSSFEWRGDDVKPEMINA 540
           +AAK+GLISGKDNIIDRSIQDAYIHAIRRAKNFIY+ENQYFLGSSFEWRG DVKPE INA
Sbjct: 481 EAAKSGLISGKDNIIDRSIQDAYIHAIRRAKNFIYVENQYFLGSSFEWRGSDVKPETINA 540

Query: 541 LHLIPKELSLKIVSKIEARERFTVYIVVPMWPEGLPEG 579
           LHLIPKELSLKIVSKIEARERF+VYIVVPMWPEGLPEG
Sbjct: 541 LHLIPKELSLKIVSKIEARERFSVYIVVPMWPEGLPEG 577

BLAST of Sgr015778 vs. ExPASy TrEMBL
Match: A0A6J1G9R5 (Phospholipase D OS=Cucurbita moschata OX=3662 GN=LOC111452246 PE=3 SV=1)

HSP 1 Score: 1134.8 bits (2934), Expect = 0.0e+00
Identity = 526/578 (91.00%), Postives = 558/578 (96.54%), Query Frame = 0

Query: 1   MAHILLHGTLHVTIYEVDRLHSSGRNIFKKLVENVEEAVGFGRGVTKLYATIDLEKARVG 60
           MAHILLHGTLHVTIYEVD+LHS  RN  K LVENVEEAVGFGRGVT+LYATIDLEKARVG
Sbjct: 1   MAHILLHGTLHVTIYEVDKLHSGARNFLKSLVENVEEAVGFGRGVTRLYATIDLEKARVG 60

Query: 61  RTRILEKEHSNPKWYETFHIYCAHMASNVIFTVKDDNPIGATLIGRAYLSVQDIIHGEEV 120
           RTR LEKEHSNPKWYETFHIYCAHMASN+IFTVKDDNPIGATLIGRAYL V++II GEEV
Sbjct: 61  RTRRLEKEHSNPKWYETFHIYCAHMASNIIFTVKDDNPIGATLIGRAYLPVKEIISGEEV 120

Query: 121 DKWVPILDERKNPIRGEPKIHVKLQYFDVTKDRNWGRGIKTPKFPGVPYTYFSQRRGCKV 180
           D+WVPILDERK PIRG+PKIHVKLQYFDVTKD+NWGRGIK+ KFPGVPYTYFSQR+GCKV
Sbjct: 121 DQWVPILDERKKPIRGDPKIHVKLQYFDVTKDQNWGRGIKSSKFPGVPYTYFSQRQGCKV 180

Query: 181 TLYQDAHVPDNFIPRIPLAGGKFYEPHRCWEDVFDAITNAKHLIYITGWSVYTEISLVRD 240
           TLYQDAHVPDNFIP+IPLAGGKFYEP+RCWED+FDAI NAKH+IYITGWSVYTEISLVRD
Sbjct: 181 TLYQDAHVPDNFIPKIPLAGGKFYEPNRCWEDIFDAIINAKHMIYITGWSVYTEISLVRD 240

Query: 241 TRRPKPGGDIILGELLKRKASEGVRVLLLVWDDKTSVPVLKKDGLMATHDEDTAKYFENT 300
            RRPKPGGD++LGELLK+KA+EGVRVLLLVW+D TSVP+LK+DGLMATHDEDTAK+F NT
Sbjct: 241 KRRPKPGGDMMLGELLKKKANEGVRVLLLVWNDTTSVPILKEDGLMATHDEDTAKFFANT 300

Query: 301 DVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPSGDPNRRRIVSFVGGIDLC 360
           DVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALP+GDPNRRRIVSFVGGIDLC
Sbjct: 301 DVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPNGDPNRRRIVSFVGGIDLC 360

Query: 361 DGRYDTPFHSLFRTLDSAHHDDFHQPNFNGTSINKGGPREPWHDIHSRLEGPVAWDVMYN 420
           DGRYDTPFHSLFRTLDSAHHDDFHQPNF GTSINKGGPREPWHDIHSRLEGPVAWDV+YN
Sbjct: 361 DGRYDTPFHSLFRTLDSAHHDDFHQPNFTGTSINKGGPREPWHDIHSRLEGPVAWDVLYN 420

Query: 421 FEQRWKKQGGKDLLVKLRDLDDIFITPSPVMFPDDFDSWNVQVFRSIDGGAAFGFPETPE 480
           FEQRW+KQGGKDLLVKLR+LD IFI PSP+MFPDDFDSWN QVFRSIDGGAAFGFP++PE
Sbjct: 421 FEQRWRKQGGKDLLVKLRELDAIFIPPSPIMFPDDFDSWNAQVFRSIDGGAAFGFPDSPE 480

Query: 481 DAAKAGLISGKDNIIDRSIQDAYIHAIRRAKNFIYIENQYFLGSSFEWRGDDVKPEMINA 540
           +AAKAGLISGKDNIIDRSIQDAYI+AIRRAKNFIYIENQYFLGSSFEWRG DVKPEMINA
Sbjct: 481 EAAKAGLISGKDNIIDRSIQDAYINAIRRAKNFIYIENQYFLGSSFEWRGTDVKPEMINA 540

Query: 541 LHLIPKELSLKIVSKIEARERFTVYIVVPMWPEGLPEG 579
           LHLIPKELSLKIVSKIEARERFTVY+VVPMWPEG PEG
Sbjct: 541 LHLIPKELSLKIVSKIEARERFTVYVVVPMWPEGFPEG 578

BLAST of Sgr015778 vs. ExPASy TrEMBL
Match: A0A6J1KB94 (Phospholipase D OS=Cucurbita maxima OX=3661 GN=LOC111493365 PE=3 SV=1)

HSP 1 Score: 1132.5 bits (2928), Expect = 0.0e+00
Identity = 526/578 (91.00%), Postives = 556/578 (96.19%), Query Frame = 0

Query: 1   MAHILLHGTLHVTIYEVDRLHSSGRNIFKKLVENVEEAVGFGRGVTKLYATIDLEKARVG 60
           MAHILLHGTLHVTIYEVD+LHS  RN  K LVENVEEAVGFGRGVT+LYATIDLEKARVG
Sbjct: 1   MAHILLHGTLHVTIYEVDKLHSGARNFLKSLVENVEEAVGFGRGVTRLYATIDLEKARVG 60

Query: 61  RTRILEKEHSNPKWYETFHIYCAHMASNVIFTVKDDNPIGATLIGRAYLSVQDIIHGEEV 120
           RTR LEKEHSNPKWYETFHIYCAHMASN+IFTVKDDNPIGATLIGRAYL V++II GEEV
Sbjct: 61  RTRRLEKEHSNPKWYETFHIYCAHMASNIIFTVKDDNPIGATLIGRAYLPVKEIISGEEV 120

Query: 121 DKWVPILDERKNPIRGEPKIHVKLQYFDVTKDRNWGRGIKTPKFPGVPYTYFSQRRGCKV 180
           D+WVPILDERK PIRGEPKIHVKLQYFDVTKD+NW RGIK+ KFPGVPYTYFSQR+GCKV
Sbjct: 121 DQWVPILDERKKPIRGEPKIHVKLQYFDVTKDQNWARGIKSSKFPGVPYTYFSQRQGCKV 180

Query: 181 TLYQDAHVPDNFIPRIPLAGGKFYEPHRCWEDVFDAITNAKHLIYITGWSVYTEISLVRD 240
           TLYQDAHVPDNFIP+IPLAGGKFYEP+RCWED+FDAI NAKH+IYITGWSVYTEISLVRD
Sbjct: 181 TLYQDAHVPDNFIPKIPLAGGKFYEPNRCWEDIFDAIINAKHMIYITGWSVYTEISLVRD 240

Query: 241 TRRPKPGGDIILGELLKRKASEGVRVLLLVWDDKTSVPVLKKDGLMATHDEDTAKYFENT 300
            RRPKPGGD++LGELLK+KASEGVRVLLLVW+D TSVP+LK+DGLMATHDEDTAK+F NT
Sbjct: 241 KRRPKPGGDVMLGELLKKKASEGVRVLLLVWNDTTSVPILKEDGLMATHDEDTAKFFANT 300

Query: 301 DVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPSGDPNRRRIVSFVGGIDLC 360
           DV CVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALP+GDPNRRRIVSFVGGIDLC
Sbjct: 301 DVQCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPNGDPNRRRIVSFVGGIDLC 360

Query: 361 DGRYDTPFHSLFRTLDSAHHDDFHQPNFNGTSINKGGPREPWHDIHSRLEGPVAWDVMYN 420
           DGRYDTPFHSLFRTLDSAHHDDFHQPNF GTSINKGGPREPWHDIHSRLEGPVAWDV+YN
Sbjct: 361 DGRYDTPFHSLFRTLDSAHHDDFHQPNFTGTSINKGGPREPWHDIHSRLEGPVAWDVLYN 420

Query: 421 FEQRWKKQGGKDLLVKLRDLDDIFITPSPVMFPDDFDSWNVQVFRSIDGGAAFGFPETPE 480
           FEQRW+KQGGKDLLVKLR+LD IFI PSPVMFPDDFDSWN QVFRSIDGGAAFGFP++PE
Sbjct: 421 FEQRWRKQGGKDLLVKLRELDAIFIPPSPVMFPDDFDSWNAQVFRSIDGGAAFGFPDSPE 480

Query: 481 DAAKAGLISGKDNIIDRSIQDAYIHAIRRAKNFIYIENQYFLGSSFEWRGDDVKPEMINA 540
           +AAKAGLISGKDNIIDRSIQDAYI+AIRRAKNFIYIENQYFLGSSFEWRG DVKPEMINA
Sbjct: 481 EAAKAGLISGKDNIIDRSIQDAYINAIRRAKNFIYIENQYFLGSSFEWRGTDVKPEMINA 540

Query: 541 LHLIPKELSLKIVSKIEARERFTVYIVVPMWPEGLPEG 579
           LHLIPKELSLKIVSKIEARERFTVY+V+PMWPEG PEG
Sbjct: 541 LHLIPKELSLKIVSKIEARERFTVYVVIPMWPEGFPEG 578

BLAST of Sgr015778 vs. ExPASy TrEMBL
Match: A0A5D3E0E7 (Phospholipase D OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G001730 PE=3 SV=1)

HSP 1 Score: 1129.8 bits (2921), Expect = 0.0e+00
Identity = 521/578 (90.14%), Postives = 558/578 (96.54%), Query Frame = 0

Query: 1   MAHILLHGTLHVTIYEVDRLHSSGRNIFKKLVENVEEAVGFGRGVTKLYATIDLEKARVG 60
           MAHILLHGTLHVTIYEVD+LHS GRN  K+LVENVEEAVGFGRG+T+LYATIDLEKARVG
Sbjct: 1   MAHILLHGTLHVTIYEVDKLHSGGRNFLKQLVENVEEAVGFGRGITRLYATIDLEKARVG 60

Query: 61  RTRILEKEHSNPKWYETFHIYCAHMASNVIFTVKDDNPIGATLIGRAYLSVQDIIHGEEV 120
           RTR LE+EHSNPKWYETFHIYCAHMASN+IFTVKDDNPIGATLIGRAYL V++II G+EV
Sbjct: 61  RTRRLEREHSNPKWYETFHIYCAHMASNIIFTVKDDNPIGATLIGRAYLPVREIIRGDEV 120

Query: 121 DKWVPILDERKNPIRGEPKIHVKLQYFDVTKDRNWGRGIKTPKFPGVPYTYFSQRRGCKV 180
           DKWVPILDE+K PIRGEPKIHVKLQ+FDVTKD+NWGRGIK+PKFPGVPYTYFSQRRGCKV
Sbjct: 121 DKWVPILDEQKKPIRGEPKIHVKLQFFDVTKDQNWGRGIKSPKFPGVPYTYFSQRRGCKV 180

Query: 181 TLYQDAHVPDNFIPRIPLAGGKFYEPHRCWEDVFDAITNAKHLIYITGWSVYTEISLVRD 240
           TLYQDAHVPDNFIP+IPLAGGKFYEPHRCWEDVFDAI NAKHLIYI+GWSVYTEISLVRD
Sbjct: 181 TLYQDAHVPDNFIPKIPLAGGKFYEPHRCWEDVFDAIMNAKHLIYISGWSVYTEISLVRD 240

Query: 241 TRRPKPGGDIILGELLKRKASEGVRVLLLVWDDKTSVPVLKKDGLMATHDEDTAKYFENT 300
            RRPKPGGDI+LGELLK+KA EGV VLLL+W+D TSVP  K++GLMATHDEDTAK+F NT
Sbjct: 241 KRRPKPGGDIMLGELLKKKAGEGVTVLLLIWNDTTSVPFFKEEGLMATHDEDTAKFFANT 300

Query: 301 DVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPSGDPNRRRIVSFVGGIDLC 360
           DVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALP+GDP++RRIVSFVGG+DLC
Sbjct: 301 DVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPNGDPSKRRIVSFVGGLDLC 360

Query: 361 DGRYDTPFHSLFRTLDSAHHDDFHQPNFNGTSINKGGPREPWHDIHSRLEGPVAWDVMYN 420
           DGRYDTPFHSLFRTLDSAHHDDFHQPNF GTSINKGGPREPWHDIHSRLEGPVAWDV++N
Sbjct: 361 DGRYDTPFHSLFRTLDSAHHDDFHQPNFAGTSINKGGPREPWHDIHSRLEGPVAWDVLHN 420

Query: 421 FEQRWKKQGGKDLLVKLRDLDDIFITPSPVMFPDDFDSWNVQVFRSIDGGAAFGFPETPE 480
           FEQRW+KQGGKDLLVKLRDLD IFITPSPV FPDDFDSWNVQVFRSIDGGAAFGFP++PE
Sbjct: 421 FEQRWRKQGGKDLLVKLRDLDQIFITPSPVTFPDDFDSWNVQVFRSIDGGAAFGFPDSPE 480

Query: 481 DAAKAGLISGKDNIIDRSIQDAYIHAIRRAKNFIYIENQYFLGSSFEWRGDDVKPEMINA 540
           +AAKAGL+SGKDN IDRSIQDAYI+AIRRAKNFIYIENQYFLGSSFEWRGDDVKPE+INA
Sbjct: 481 EAAKAGLVSGKDNTIDRSIQDAYINAIRRAKNFIYIENQYFLGSSFEWRGDDVKPEVINA 540

Query: 541 LHLIPKELSLKIVSKIEARERFTVYIVVPMWPEGLPEG 579
           LHLIPKELSLKI+SKIEARERFTVYI+VPMWPEG PEG
Sbjct: 541 LHLIPKELSLKIMSKIEARERFTVYILVPMWPEGFPEG 578

BLAST of Sgr015778 vs. ExPASy TrEMBL
Match: A7XQW1 (Phospholipase D OS=Cucumis melo var. inodorus OX=357961 GN=PLDa2 PE=2 SV=1)

HSP 1 Score: 1129.8 bits (2921), Expect = 0.0e+00
Identity = 521/578 (90.14%), Postives = 558/578 (96.54%), Query Frame = 0

Query: 1   MAHILLHGTLHVTIYEVDRLHSSGRNIFKKLVENVEEAVGFGRGVTKLYATIDLEKARVG 60
           MAHILLHGTLHVTIYEVD+LHS GRN  K+LVENVEEAVGFGRG+T+LYATIDLEKARVG
Sbjct: 1   MAHILLHGTLHVTIYEVDKLHSGGRNFLKQLVENVEEAVGFGRGITRLYATIDLEKARVG 60

Query: 61  RTRILEKEHSNPKWYETFHIYCAHMASNVIFTVKDDNPIGATLIGRAYLSVQDIIHGEEV 120
           RTR LE+EHSNPKWYETFHIYCAHMASN+IFTVKDDNPIGATLIGRAYL V++II G+EV
Sbjct: 61  RTRRLEREHSNPKWYETFHIYCAHMASNIIFTVKDDNPIGATLIGRAYLPVREIIRGDEV 120

Query: 121 DKWVPILDERKNPIRGEPKIHVKLQYFDVTKDRNWGRGIKTPKFPGVPYTYFSQRRGCKV 180
           DKWVPILDE+K PIRGEPKIHVKLQ+FDVTKD+NWGRGIK+PKFPGVPYTYFSQRRGCKV
Sbjct: 121 DKWVPILDEQKKPIRGEPKIHVKLQFFDVTKDQNWGRGIKSPKFPGVPYTYFSQRRGCKV 180

Query: 181 TLYQDAHVPDNFIPRIPLAGGKFYEPHRCWEDVFDAITNAKHLIYITGWSVYTEISLVRD 240
           TLYQDAHVPDNFIP+IPLAGGKFYEPHRCWEDVFDAI NAKHLIYI+GWSVYTEISLVRD
Sbjct: 181 TLYQDAHVPDNFIPKIPLAGGKFYEPHRCWEDVFDAIMNAKHLIYISGWSVYTEISLVRD 240

Query: 241 TRRPKPGGDIILGELLKRKASEGVRVLLLVWDDKTSVPVLKKDGLMATHDEDTAKYFENT 300
            RRPKPGGDI+LGELLK+KA EGV VLLL+W+D TSVP  K++GLMATHDEDTAK+F NT
Sbjct: 241 KRRPKPGGDIMLGELLKKKAGEGVTVLLLIWNDTTSVPFFKEEGLMATHDEDTAKFFANT 300

Query: 301 DVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPSGDPNRRRIVSFVGGIDLC 360
           DVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALP+GDP++RRIVSFVGG+DLC
Sbjct: 301 DVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPNGDPSKRRIVSFVGGLDLC 360

Query: 361 DGRYDTPFHSLFRTLDSAHHDDFHQPNFNGTSINKGGPREPWHDIHSRLEGPVAWDVMYN 420
           DGRYDTPFHSLFRTLDSAHHDDFHQPNF GTSINKGGPREPWHDIHSRLEGPVAWDV++N
Sbjct: 361 DGRYDTPFHSLFRTLDSAHHDDFHQPNFAGTSINKGGPREPWHDIHSRLEGPVAWDVLHN 420

Query: 421 FEQRWKKQGGKDLLVKLRDLDDIFITPSPVMFPDDFDSWNVQVFRSIDGGAAFGFPETPE 480
           FEQRW+KQGGKDLLVKLRDLD IFITPSPV FPDDFDSWNVQVFRSIDGGAAFGFP++PE
Sbjct: 421 FEQRWRKQGGKDLLVKLRDLDQIFITPSPVTFPDDFDSWNVQVFRSIDGGAAFGFPDSPE 480

Query: 481 DAAKAGLISGKDNIIDRSIQDAYIHAIRRAKNFIYIENQYFLGSSFEWRGDDVKPEMINA 540
           +AAKAGL+SGKDN IDRSIQDAYI+AIRRAKNFIYIENQYFLGSSFEWRGDDVKPE+INA
Sbjct: 481 EAAKAGLVSGKDNTIDRSIQDAYINAIRRAKNFIYIENQYFLGSSFEWRGDDVKPEVINA 540

Query: 541 LHLIPKELSLKIVSKIEARERFTVYIVVPMWPEGLPEG 579
           LHLIPKELSLKI+SKIEARERFTVYI+VPMWPEG PEG
Sbjct: 541 LHLIPKELSLKIMSKIEARERFTVYILVPMWPEGFPEG 578

BLAST of Sgr015778 vs. TAIR 10
Match: AT3G15730.1 (phospholipase D alpha 1 )

HSP 1 Score: 980.3 bits (2533), Expect = 6.6e-286
Identity = 449/580 (77.41%), Postives = 507/580 (87.41%), Query Frame = 0

Query: 1   MAHILLHGTLHVTIYEVDRLHSSG--RNIFKKLVENVEEAVGFGRGVTKLYATIDLEKAR 60
           MA  LLHGTLH TIYEVD LH  G  +    K++ NVEE +G G+G T+LYATIDL+KAR
Sbjct: 1   MAQHLLHGTLHATIYEVDALHGGGVRQGFLGKILANVEETIGVGKGETQLYATIDLQKAR 60

Query: 61  VGRTRILEKEHSNPKWYETFHIYCAHMASNVIFTVKDDNPIGATLIGRAYLSVQDIIHGE 120
           VGRTR ++ E  NPKWYE+FHIYCAH+AS++IFTVKDDNPIGATLIGRAY+ V  +I+GE
Sbjct: 61  VGRTRKIKNEPKNPKWYESFHIYCAHLASDIIFTVKDDNPIGATLIGRAYIPVDQVINGE 120

Query: 121 EVDKWVPILDERKNPIRGEPKIHVKLQYFDVTKDRNWGRGIKTPKFPGVPYTYFSQRRGC 180
           EVD+WV ILD  +NPI+G  KIHVKLQYF V +DRNW  GIK+ KFPGVPYT+FSQR+GC
Sbjct: 121 EVDQWVEILDNDRNPIQGGSKIHVKLQYFHVEEDRNWNMGIKSAKFPGVPYTFFSQRQGC 180

Query: 181 KVTLYQDAHVPDNFIPRIPLAGGKFYEPHRCWEDVFDAITNAKHLIYITGWSVYTEISLV 240
           KV+LYQDAH+PDNF+PRIPLAGGK YEP RCWED+FDAI+NAKHLIYITGWSVY EI+LV
Sbjct: 181 KVSLYQDAHIPDNFVPRIPLAGGKNYEPQRCWEDIFDAISNAKHLIYITGWSVYAEIALV 240

Query: 241 RDTRRPKPGGDIILGELLKRKASEGVRVLLLVWDDKTSVPVLKKDGLMATHDEDTAKYFE 300
           RD+RRPKPGGD+ +GELLK+KASEGVRVLLLVWDD+TSV VLKKDGLMATHDE+T  +F 
Sbjct: 241 RDSRRPKPGGDVTIGELLKKKASEGVRVLLLVWDDRTSVDVLKKDGLMATHDEETENFFR 300

Query: 301 NTDVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPS-GDPNRRRIVSFVGGI 360
            +DVHC+LCPRNPDDG +++Q + + TMFTHHQKIVVVDS +PS G    RRIVSFVGGI
Sbjct: 301 GSDVHCILCPRNPDDGGSIVQSLQISTMFTHHQKIVVVDSEMPSRGGSEMRRIVSFVGGI 360

Query: 361 DLCDGRYDTPFHSLFRTLDSAHHDDFHQPNFNGTSINKGGPREPWHDIHSRLEGPVAWDV 420
           DLCDGRYDTPFHSLFRTLD+ HHDDFHQPNF G +I KGGPREPWHDIHSRLEGP+AWDV
Sbjct: 361 DLCDGRYDTPFHSLFRTLDTVHHDDFHQPNFTGAAITKGGPREPWHDIHSRLEGPIAWDV 420

Query: 421 MYNFEQRWKKQGGKDLLVKLRDLDDIFITPSPVMFPDDFDSWNVQVFRSIDGGAAFGFPE 480
           MYNFEQRW KQGGKD+LVKLRDL DI ITPSPVMF +D D WNVQ+FRSIDGGAA GFPE
Sbjct: 421 MYNFEQRWSKQGGKDILVKLRDLSDIIITPSPVMFQEDHDVWNVQLFRSIDGGAAAGFPE 480

Query: 481 TPEDAAKAGLISGKDNIIDRSIQDAYIHAIRRAKNFIYIENQYFLGSSFEWRGDDVKPEM 540
           +PE AA+AGL+SGKDNIIDRSIQDAYIHAIRRAK+FIY+ENQYFLGSSF W  D + PE 
Sbjct: 481 SPEAAAEAGLVSGKDNIIDRSIQDAYIHAIRRAKDFIYVENQYFLGSSFAWAADGITPED 540

Query: 541 INALHLIPKELSLKIVSKIEARERFTVYIVVPMWPEGLPE 578
           INALHLIPKELSLKIVSKIE  E+F VY+VVPMWPEGLPE
Sbjct: 541 INALHLIPKELSLKIVSKIEKGEKFRVYVVVPMWPEGLPE 580

BLAST of Sgr015778 vs. TAIR 10
Match: AT1G52570.1 (phospholipase D alpha 2 )

HSP 1 Score: 973.8 bits (2516), Expect = 6.2e-284
Identity = 441/579 (76.17%), Postives = 504/579 (87.05%), Query Frame = 0

Query: 1   MAHILLHGTLHVTIYEVDRLHSSG--RNIFKKLVENVEEAVGFGRGVTKLYATIDLEKAR 60
           M   LLHG LH TIYEVD LH+ G        ++ NVEE +G G+G T+LYATIDLEKAR
Sbjct: 1   MEECLLHGRLHATIYEVDHLHAEGGRSGFLGSILANVEETIGVGKGETQLYATIDLEKAR 60

Query: 61  VGRTRILEKEHSNPKWYETFHIYCAHMASNVIFTVKDDNPIGATLIGRAYLSVQDIIHGE 120
           VGRTR + KE  NPKW+E+FHIYC HMA +VIFTVKD NPIGATLIGR Y+ V+DI+HGE
Sbjct: 61  VGRTRKITKEPKNPKWFESFHIYCGHMAKHVIFTVKDANPIGATLIGRGYIPVEDILHGE 120

Query: 121 EVDKWVPILDERKNPIRGEPKIHVKLQYFDVTKDRNWGRGIKTPKFPGVPYTYFSQRRGC 180
           EVD+WV ILD  KNPI G  KIHVKLQYF V KD+NW RGIK+ KFPGVPYT+FSQRRGC
Sbjct: 121 EVDRWVDILDNEKNPIAGGSKIHVKLQYFGVEKDKNWNRGIKSAKFPGVPYTFFSQRRGC 180

Query: 181 KVTLYQDAHVPDNFIPRIPLAGGKFYEPHRCWEDVFDAITNAKHLIYITGWSVYTEISLV 240
           KV+LYQDAH+P NF+P+IPLAGGK YEPHRCWED+FDAITNAKHLIYITGWSVYTEISLV
Sbjct: 181 KVSLYQDAHIPGNFVPKIPLAGGKNYEPHRCWEDIFDAITNAKHLIYITGWSVYTEISLV 240

Query: 241 RDTRRPKPGGDIILGELLKRKASEGVRVLLLVWDDKTSVPVLKKDGLMATHDEDTAKYFE 300
           RD+RRPK GGD+ +GELLK+KASEGV+V+LLVWDD+TSV +LKKDGLMATHDE+T  +F 
Sbjct: 241 RDSRRPKQGGDVTVGELLKKKASEGVKVILLVWDDRTSVDLLKKDGLMATHDEETENFFR 300

Query: 301 NTDVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPSGDPNRRRIVSFVGGID 360
            TDV+C+LCPRNPDDG +++Q++ + TMFTHHQKIVVVDS +PSG    RRIVSFVGG+D
Sbjct: 301 GTDVNCILCPRNPDDGGSIVQNLQISTMFTHHQKIVVVDSEMPSGGSRSRRIVSFVGGLD 360

Query: 361 LCDGRYDTPFHSLFRTLDSAHHDDFHQPNFNGTSINKGGPREPWHDIHSRLEGPVAWDVM 420
           LCDGRYDTPFHSLFRTLD+AHHDDFHQPNF G +I KGGPREPWHDIH RLEGP+AWDV+
Sbjct: 361 LCDGRYDTPFHSLFRTLDTAHHDDFHQPNFTGAAITKGGPREPWHDIHCRLEGPIAWDVL 420

Query: 421 YNFEQRWKKQGGKDLLVKLRDLDDIFITPSPVMFPDDFDSWNVQVFRSIDGGAAFGFPET 480
           YNFEQRW +QGGKD+LVK+R+L DI I PSPV+F +D D WNVQ+FRSIDGGAA GFP++
Sbjct: 421 YNFEQRWSRQGGKDILVKMRELGDIIIPPSPVLFSEDHDVWNVQLFRSIDGGAAAGFPDS 480

Query: 481 PEDAAKAGLISGKDNIIDRSIQDAYIHAIRRAKNFIYIENQYFLGSSFEWRGDDVKPEMI 540
           PE AA+AGL+SGKDNIIDRSIQDAYIHAIRRAK+FIYIENQYFLGSSF W  D +KPE I
Sbjct: 481 PEAAAEAGLVSGKDNIIDRSIQDAYIHAIRRAKDFIYIENQYFLGSSFAWSADGIKPEEI 540

Query: 541 NALHLIPKELSLKIVSKIEARERFTVYIVVPMWPEGLPE 578
           NALHLIPKELSLKIVSKI+A E+F VY+VVPMWPEG+PE
Sbjct: 541 NALHLIPKELSLKIVSKIKAGEKFKVYVVVPMWPEGIPE 579

BLAST of Sgr015778 vs. TAIR 10
Match: AT5G25370.1 (phospholipase D alpha 3 )

HSP 1 Score: 738.4 bits (1905), Expect = 4.4e-213
Identity = 349/592 (58.95%), Postives = 437/592 (73.82%), Query Frame = 0

Query: 4   ILLHGTLHVTIYEVDRLHSSGR-----------------NIFKKLVENVEEAVGFGRGVT 63
           +LLHGTL V IY +D+LH   R                 +  K+L ++     G      
Sbjct: 5   LLLHGTLEVKIYRIDKLHQRSRFNLCGKGNKEPTGKKTQSQIKRLTDSCTSLFG-----G 64

Query: 64  KLYATIDLEKARVGRTRILEKEHSNPKWYETFHIYCAHMASNVIFTVKDDNPIGATLIGR 123
            LYATIDL+++RV RT +      +PKW ++FH+Y AH  S +IFTVK+D P+ A+LIGR
Sbjct: 65  HLYATIDLDRSRVARTMM----RRHPKWLQSFHVYTAHSISKIIFTVKEDEPVSASLIGR 124

Query: 124 AYLSVQDIIHGEEVDKWVPILDERKNPIRGEPKIHVKLQYFDVTKDRNWGRGIKTPKFPG 183
           AYL V ++I G+ +D+W+ ILDE + PI+G  K+HV++++  VT+D NW +GI  P F G
Sbjct: 125 AYLPVTEVITGQPIDRWLDILDENRRPIQGGSKLHVRVKFTHVTQDVNWNKGIILPSFNG 184

Query: 184 VPYTYFSQRRGCKVTLYQDAHVPDNFIPRIPLAGGK-FYEPHRCWEDVFDAITNAKHLIY 243
           VP  YF+QR GCKVTLYQDAHV + + P + L GG+  Y+ HRCWE++FDAI  AKHLIY
Sbjct: 185 VPNAYFNQREGCKVTLYQDAHVLNEY-PDVTLTGGQVIYKHHRCWEEIFDAIWEAKHLIY 244

Query: 244 ITGWSVYTEISLVRDTRRPKPGGDIILGELLKRKASEGVRVLLLVWDDKTSVPVLKKDGL 303
           I GWSV T+++LVRD +R +PGGD+ LGELLK+KA E V VL+LVWDD+TS  V K+DGL
Sbjct: 245 IAGWSVNTDVTLVRDPKRTRPGGDLKLGELLKKKAEENVTVLMLVWDDRTSHEVFKRDGL 304

Query: 304 MATHDEDTAKYFENTDVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPSGDP 363
           M THD++T  YF+NT V CVLCPRNPD+G +++Q   V TMFTHHQK +VVDS +  G  
Sbjct: 305 MMTHDQETYDYFKNTKVRCVLCPRNPDNGDSIVQGFEVATMFTHHQKTIVVDSEV-DGSL 364

Query: 364 NRRRIVSFVGGIDLCDGRYDTPFHSLFRTLDSAHHDDFHQPNFNGTSINKGGPREPWHDI 423
            +RRIVSF+GGIDLCDGRYDT  H LF TL+S H +DFHQPNF+G SI KGGPREPWHDI
Sbjct: 365 TKRRIVSFLGGIDLCDGRYDTVEHPLFGTLNSVHANDFHQPNFDGASIKKGGPREPWHDI 424

Query: 424 HSRLEGPVAWDVMYNFEQRWKKQG-GKDLLVKLRDLDDIFITPSPVMFPDDFDSWNVQVF 483
           H +L+GP AWDV+YNFEQRW KQG G+  L+ +  L +I + P P++ PD+ + W VQVF
Sbjct: 425 HCKLDGPAAWDVLYNFEQRWMKQGSGRRYLISMAQLAEITVPPLPIVQPDNEEGWTVQVF 484

Query: 484 RSIDGGAAFGFPETPEDAAKAGLISGKDNIIDRSIQDAYIHAIRRAKNFIYIENQYFLGS 543
           RSID GA  GFPE P +AA  GLISGKDN+I+RSIQDAY++AIRRAKNFIYIENQYFLGS
Sbjct: 485 RSIDDGAVEGFPEDPREAASIGLISGKDNVIERSIQDAYVNAIRRAKNFIYIENQYFLGS 544

Query: 544 SFEWRGDDVKPEMINALHLIPKELSLKIVSKIEARERFTVYIVVPMWPEGLP 577
           SF W   D+    INAL LIPKE+SLKIVSKIEA ERF+VYIV+P+WPEG P
Sbjct: 545 SFGWNSRDINLNEINALQLIPKEISLKIVSKIEAGERFSVYIVIPLWPEGKP 585

BLAST of Sgr015778 vs. TAIR 10
Match: AT2G42010.1 (phospholipase D beta 1 )

HSP 1 Score: 520.8 bits (1340), Expect = 1.4e-147
Identity = 267/605 (44.13%), Postives = 379/605 (62.64%), Query Frame = 0

Query: 4   ILLHGTLHVTIY------EVDRLHSSGRNIFKKLVENVEEAVGFGRGVTKL----YATID 63
           +LLHG L + IY       +D  H +  ++F +L   +E     G+  +K+    Y ++ 
Sbjct: 269 LLLHGNLDIWIYHAKNLPNMDMFHKTLGDMFGRLPGKIE-----GQLTSKITSDPYVSVS 328

Query: 64  LEKARVGRTRILEKEHSNPKWYETFHIYCAHMASNVIFTVKDDNPIGATLIGRAYLSVQD 123
           +  A +GRT ++     NP W + F++  AH A+ V F VKD + +G+ LIG   + V+ 
Sbjct: 329 VAGAVIGRTYVMSNS-ENPVWMQHFYVPVAHHAAEVHFVVKDSDVVGSQLIGLVTIPVEQ 388

Query: 124 IIHGEEVDKWVPILDERKNPIRGEPKIHVKLQYFDVTKDRNWGRGIKT-PKFPGVPYTYF 183
           I  G +++   PIL+    P +    + + +QY  + K   +  G+   P + GVP TYF
Sbjct: 389 IYSGAKIEGTYPILNSNGKPCKPGANLSLSIQYTPMDKLSVYHHGVGAGPDYQGVPGTYF 448

Query: 184 SQRRGCKVTLYQDAHVPDNFIPRIPLAGGKFYEPHRCWEDVFDAITNAKHLIYITGWSVY 243
             R+G  V LYQDAHVP+  +P I L  G  YE  +CW D+FDAI  A+ LIYITGWSV+
Sbjct: 449 PLRKGGTVRLYQDAHVPEGMLPGIRLDNGMSYEHGKCWHDMFDAIRQARRLIYITGWSVW 508

Query: 244 TEISLVRDTRRPKPGGDIILGELLKRKASEGVRVLLLVWDDKTSVPVL--KKDGLMATHD 303
            ++ L+RD  +  P  +  LGELL+ K+ EGVRVLLL+WDD TS  +L  K DG+MATHD
Sbjct: 509 HKVKLIRD--KLGPASECTLGELLRSKSQEGVRVLLLIWDDPTSRSILGYKTDGVMATHD 568

Query: 304 EDTAKYFENTDVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPSGDPNRRRI 363
           E+T ++F+++ V  +LCPRN     + ++   VGT++THHQK V+VD+       NRR+I
Sbjct: 569 EETRRFFKHSSVQVLLCPRNAGKRHSWVKQREVGTIYTHHQKNVIVDA---DAGGNRRKI 628

Query: 364 VSFVGGIDLCDGRYDTPFHSLFRTLDSAHHDDFHQPNFNGTSINKGGPREPWHDIHSRLE 423
           ++FVGG+DLCDGRYDTP H LFRTL + H DDFH P F G     G PREPWHD+HS+++
Sbjct: 629 IAFVGGLDLCDGRYDTPQHPLFRTLQTIHKDDFHNPTFTGNL--SGCPREPWHDLHSKID 688

Query: 424 GPVAWDVMYNFEQRW----KKQGGK-------DLLVKLRDLDDIF-ITPSPVMFPDDFDS 483
           GP A+DV+ NFE+RW    K  G K       D L+++  + DI  ++ +P +  +D ++
Sbjct: 689 GPAAYDVLTNFEERWLKAAKPSGIKKFKTSYDDALLRIDRIPDILGVSDTPTVSENDPEA 748

Query: 484 WNVQVFRSIDGGAAFGFPETPEDAAKAGLISGKDNIIDRSIQDAYIHAIRRAKNFIYIEN 543
           W+VQ+FRSID  +  GFP+ P+DA    L+ GK+ +ID SI  AY+ AIR A++FIYIEN
Sbjct: 749 WHVQIFRSIDSNSVKGFPKDPKDATCKNLVCGKNVLIDMSIHTAYVKAIRAAQHFIYIEN 808

Query: 544 QYFLGSSFEWRGDDVKPEMINALHLIPKELSLKIVSKIEARERFTVYIVVPMWPEGLPEG 584
           QYF+GSS+ W       + I A +LIP E++LKI  KI A ERF  YIV+PMWPEG+P G
Sbjct: 809 QYFIGSSYNWNAH----KDIGANNLIPMEIALKIAEKIRANERFAAYIVIPMWPEGVPTG 856

BLAST of Sgr015778 vs. TAIR 10
Match: AT4G00240.1 (phospholipase D beta 2 )

HSP 1 Score: 500.7 bits (1288), Expect = 1.5e-141
Identity = 257/601 (42.76%), Postives = 365/601 (60.73%), Query Frame = 0

Query: 4   ILLHGTLHV------TIYEVDRLHSSGRNIFKKLVENVEEAVGFGRGVTKLYATIDLEKA 63
           +LLHG L +       +  +D  H +   +F  +   +E  +   +  +  Y +I +  A
Sbjct: 113 LLLHGNLDIWVSCANNLPNLDLFHKTLGVVFGGMTNMIEGQLS-KKITSDPYVSISVAGA 172

Query: 64  RVGRTRILEKEHSNPKWYETFHIYCAHMASNVIFTVKDDNPIGATLIGRAYLSVQDIIHG 123
            +GRT ++     NP W + F++  AH A+ V F VKD + +G+ LIG   + V+ I  G
Sbjct: 173 VIGRTYVISNS-ENPVWQQHFYVPVAHHAAEVHFVVKDSDAVGSQLIGIVTIPVEQIYSG 232

Query: 124 EEVDKWVPILDERKNPIRGEPKIHVKLQYFDVTKDRNWGRGIKT-PKFPGVPYTYFSQRR 183
             ++    I D    P +    + + +QY  + K   +  G+   P + GVP TYF  R 
Sbjct: 233 ARIEGTYSIRDSNGKPCKPGATLSLSIQYTSMNKLSVYHSGVGAGPYYQGVPGTYFPLRE 292

Query: 184 GCKVTLYQDAHVPDNFIPRIPLAGGKFYEPHRCWEDVFDAITNAKHLIYITGWSVYTEIS 243
           G  VTLYQDAHVP+  +P I L  G  YE  +CW D+F AI  A+ LIYITGWSV+  + 
Sbjct: 293 GGSVTLYQDAHVPEGMLPGIKLGNGMCYEHGKCWHDMFHAICQARRLIYITGWSVWHNVR 352

Query: 244 LVRDTRRPKPGGDIILGELLKRKASEGVRVLLLVWDDKTSVPVL--KKDGLMATHDEDTA 303
           LVRD  +  P  +  LGELL+ K+ EGVRVLLLVWDD TS  +L    DG+M THDE+T 
Sbjct: 353 LVRD--KEDPSSECRLGELLRSKSQEGVRVLLLVWDDPTSQNILGYMTDGVMGTHDEETR 412

Query: 304 KYFENTDVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPSGDPNRRRIVSFV 363
           ++F+++ V  +LCPRN     + ++   VGT++THHQK ++VD+       NRR+IV+FV
Sbjct: 413 RFFKDSSVQVLLCPRNAGKRHSWVKQREVGTIYTHHQKNLIVDA---DAGGNRRKIVAFV 472

Query: 364 GGIDLCDGRYDTPFHSLFRTLDSAHHDDFHQPNFNGTSINKGGPREPWHDIHSRLEGPVA 423
           GG+DLCDGRYDTP H LFRTL + H+ D+H P F G     G PREPWHD+HS+++GP A
Sbjct: 473 GGLDLCDGRYDTPQHPLFRTLQTDHNGDYHNPTFTGNV--SGCPREPWHDLHSKIDGPAA 532

Query: 424 WDVMYNFEQRWKKQGG-----------KDLLVKLRDLDDIF-ITPSPVMFPDDFDSWNVQ 483
           +DV+ NFE+RW K               D L+++  + DI  +  +P +  +D ++W+VQ
Sbjct: 533 YDVLTNFEERWLKAAKPHRINKLKTSYDDALLRIDRIPDILRVLDAPTVSANDPEAWHVQ 592

Query: 484 VFRSIDGGAAFGFPETPEDAAKAGLISGKDNIIDRSIQDAYIHAIRRAKNFIYIENQYFL 543
           +FRSID  +  GFP+ P+ A    L+ GK+ +ID SI  AY+ AIR A++FIYIENQYF+
Sbjct: 593 IFRSIDSNSVKGFPKDPKYATSKNLVCGKNVLIDMSIHTAYVKAIRAAQHFIYIENQYFI 652

Query: 544 GSSFEWRGDDVKPEMINALHLIPKELSLKIVSKIEARERFTVYIVVPMWPEGLPEGEQFR 584
           GSS++W       + I A +LIP E++LKI  KI A+ERF  YIV+PMWPEG+P G   +
Sbjct: 653 GSSYDWNAH----KDIGANNLIPMEIALKIADKIRAKERFAAYIVIPMWPEGVPTGAATQ 700

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038880369.10.0e+0093.43phospholipase D alpha 1 [Benincasa hispida] >XP_038880372.1 phospholipase D alph... [more]
XP_022153673.10.0e+0093.08phospholipase D alpha 1-like [Momordica charantia][more]
KAG7036505.10.0e+0091.18Phospholipase D alpha 1 [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6606795.10.0e+0091.18Phospholipase D alpha 1, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023523927.10.0e+0091.18phospholipase D alpha 1-like [Cucurbita pepo subsp. pepo] >XP_023523928.1 phosph... [more]
Match NameE-valueIdentityDescription
Q411422.0e-30381.49Phospholipase D alpha 1 OS=Ricinus communis OX=3988 GN=PLD1 PE=1 SV=1[more]
P863871.1e-29378.89Phospholipase D alpha 1 OS=Carica papaya OX=3649 GN=PLD1 PE=1 SV=1[more]
O048652.7e-29278.93Phospholipase D alpha 1 OS=Vigna unguiculata OX=3917 GN=PLD1 PE=2 SV=1[more]
P934005.5e-28576.64Phospholipase D alpha 1 OS=Nicotiana tabacum OX=4097 GN=PLD1 PE=2 SV=2[more]
Q388829.3e-28577.41Phospholipase D alpha 1 OS=Arabidopsis thaliana OX=3702 GN=PLDALPHA1 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1DI410.0e+0093.08Phospholipase D OS=Momordica charantia OX=3673 GN=LOC111021129 PE=3 SV=1[more]
A0A6J1G9R50.0e+0091.00Phospholipase D OS=Cucurbita moschata OX=3662 GN=LOC111452246 PE=3 SV=1[more]
A0A6J1KB940.0e+0091.00Phospholipase D OS=Cucurbita maxima OX=3661 GN=LOC111493365 PE=3 SV=1[more]
A0A5D3E0E70.0e+0090.14Phospholipase D OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G0017... [more]
A7XQW10.0e+0090.14Phospholipase D OS=Cucumis melo var. inodorus OX=357961 GN=PLDa2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT3G15730.16.6e-28677.41phospholipase D alpha 1 [more]
AT1G52570.16.2e-28476.17phospholipase D alpha 2 [more]
AT5G25370.14.4e-21358.95phospholipase D alpha 3 [more]
AT2G42010.11.4e-14744.13phospholipase D beta 1 [more]
AT4G00240.11.5e-14142.76phospholipase D beta 2 [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001736Phospholipase D/TransphosphatidylaseSMARTSM00155pld_4coord: 325..363
e-value: 0.0065
score: 25.7
IPR001736Phospholipase D/TransphosphatidylasePFAMPF00614PLDccoord: 325..363
e-value: 2.1E-10
score: 40.3
IPR001736Phospholipase D/TransphosphatidylasePROSITEPS50035PLDcoord: 325..363
score: 10.931199
IPR000008C2 domainSMARTSM00239C2_3ccoord: 9..123
e-value: 1.9E-12
score: 57.4
IPR000008C2 domainPFAMPF00168C2coord: 8..126
e-value: 1.2E-14
score: 54.4
IPR000008C2 domainPROSITEPS50004C2coord: 1..124
score: 14.036459
IPR035892C2 domain superfamilyGENE3D2.60.40.150C2 domaincoord: 6..155
e-value: 1.6E-15
score: 59.3
IPR035892C2 domain superfamilySUPERFAMILY49562C2 domain (Calcium/lipid-binding domain, CaLB)coord: 6..149
NoneNo IPR availableGENE3D3.30.870.10Endonuclease Chain Acoord: 201..439
e-value: 2.7E-23
score: 84.2
NoneNo IPR availableGENE3D3.30.870.10Endonuclease Chain Acoord: 478..581
e-value: 5.7E-6
score: 28.0
NoneNo IPR availablePANTHERPTHR18896:SF162PHOSPHOLIPASE Dcoord: 2..578
NoneNo IPR availableCDDcd04015C2_plant_PLDcoord: 2..147
e-value: 2.30123E-66
score: 211.394
NoneNo IPR availableSUPERFAMILY56024Phospholipase D/nucleasecoord: 484..576
NoneNo IPR availableSUPERFAMILY56024Phospholipase D/nucleasecoord: 150..435
IPR015679Phospholipase D familyPANTHERPTHR18896PHOSPHOLIPASE Dcoord: 2..578

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr015778.1Sgr015778.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046470 phosphatidylcholine metabolic process
biological_process GO:0009395 phospholipid catabolic process
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0005886 plasma membrane
cellular_component GO:0009536 plastid
cellular_component GO:0005773 vacuole
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0070290 N-acylphosphatidylethanolamine-specific phospholipase D activity
molecular_function GO:0004630 phospholipase D activity
molecular_function GO:0003824 catalytic activity