Sgr015688 (gene) Monk fruit (Qingpiguo) v1

Overview


Name	Sgr015688
Type	gene
Organism	Siraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description	Methylenetetrahydrofolate reductase
Location	tig00004872: 402653 .. 408975 (-)
RNA-Seq Expression	Sgr015688
Synteny	Sgr015688

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GTAAAACACATCAGGGAGAAATATGGTGACTACTTCGGCATTACTGTAGCTGGCTATCCAGGTTTCTTTTATTTTCTATGTGGTTTCTTTTATTTTCTATGTTTTGATTGGAAGTCGCAGATCCACCTACTGATGCGTTCCTTCTGTAAACTAACGAAATAATAATGCTTACTTCCATTCTGTGTTCTTCTCAGAGGCACATCCGGACGTGATTGGAAGCAATGGCGTGGCGACGCCGGATGGCTACCAGAGCGATCTGGCTTATCTTAAGAGAAAGGTGACTCTCATCTTATAGTAATTTTAATTCCTCTTCCCCTGCCATTACATGGGCTACTCTTATATATGCTAATTTCTTCTTTTTACTTGCTGCCATTTTGTTTTTTCATTTTGATTCTTTCCAGTTCGATGCTGGGGCTGATCTGATTGTTACCCAACTATTCTATGACACTGACATTTTCCTCAAGTTTGTCAATGATTGTCGTCAAATTGGAATTACTTGTCCGATTGTACCTGGTATTATGCCCATCAATAATTACAAAGGTTTCTTACGCATGACTGGCTTTTGCAAAACGAAGGTACTAACCTCTCTCTACCTCAATTGATCATCTTATACTCAGATGTATCTGCTTTCTTGAATTTTCTGTATTATATCATTAAAAGGGGGGAAAAAAAGAAACATCATCTTGATCTCTATCTTGAAGCAAACACTACCTTGATGTCTTCATTCCTTTTGATAGTTGCGATTAGAAGGCTGCAGCCACTGCTTGCCATTTCACTCACCGTCGTTGGCACTACGAGTCATTTTCACGCAGTTGCTTTAAAATATTCACTGGAATTTCCTGCAATCTGTGGTTGCTTTTGAAAATTTAAGCTGCACTTCTCATGTAGTTGCTGCCAGTTCACCACCCACCACATGTGCTCCTGTATTTTAGAAATTCATACTTTTCACTGCAGATTATCTTTAATGGCCTTGATATAGTTATATCAATTGATATTGATGGGTCACCATCAAGGAACTTGTAACTTAAAACCATATTTGTGTGGAGAAAGCTGACTCCGTCTGGTTGTATGCTTTGATGAAATCCTCATTCTAGAAGAGGAAAAGGGAAAGATAGAGAGATATGTCATCATTAATATTCAATAAAGAATGTCAACTGCGACATACTTTCTTGAACTCCCTTCTCTTTTCTTAAATGAAGATGGGGAAGGATCCCAATTGTGTCATAGTCCTTATTCTTTGATGCGGACTGAAGGCCATGGGTTGGAGGATATATCTCTCCCTGCATTATCCCTAAAAATTTGCAATCACTGATCATCATATTTTCTTTTTTCTTCCCTAAAGAAAATTAATTTGTTCTATAATGTTAAATTGTTTCTCTTTGACCTTGATGTATGACTTTGGAAAACTTGCAGATTCCGGATGAGATCACTGCTGCCTTAGAGCCTATCAAGGACAACGAAGAAGCTGTGAAAGCTTATGGAATTCACCTTGGAACTGAGATGTGCAAGAAGATTTTGGCTCATGGAATCAAGACTCTACATCTTTATACATTGAACATGGAGAAATCAGCATTGGCTATACTAATGGTATATTCCAGTATTCCGTTCGCATTCTAGTTTTACTCACAAACGCACATTTGTATGTACTTCCAATTATATTTTGTATGTGTTATAATTTTGTCTATGGATGCTCTGGTACCATGGAAATTGCTGAAGCTTTTGTTAATGTAACCTCATATTCTTTACTTAATATTAGTGGGGGATGCGTTTCATTGAATTTGGCTTCCGTTCATATTATTGGTTCATGTGTTACTTGGACAACTGAGAATCTGCATTTTGAAGTAAACTTGTAAGGAGCTATAAAGTAATTTATGTAATGAAAACTTCTGAATGTAAATAGGTGATAAGTCTATTAAAAGTGAGTTTTGTAAACACAGTTACTATTACTAACCTATTCCTTGATGAGCAGTGGATAAACTTTTCTTATACTCACATGAACTTGATGCATATATTTTTCCGAAGATAAGATAAACAATACATAACATAGATAATTTTTTGTTGCTTCGATGCCCCTAGGTTTCTTTTTCTAATATAAAGGAATTTAGACTTTTTCTATTCTTTTATTTTTTTGTACAAAAATTATTTTTATTTTCTTAATTTTCATTTATGGAGGCTTGTAGCTTTTGGGATTAAATCCATGTCCAAGTGCACTTCTACTCATGTTTTATACTTCTGTCTCACTAAAAACACATGGCAGAATCTTGGTCTGATTGAAGAGAGCAAAGTTGCAAGATCTTTACCTTGGAGGCGCCCCACAAATGTTTTCCGTATCAAGGAAGATGTCCGCCCAATCTTTTGGTATCACATATTCTTATTGCAATATTTGGGATAAATTTGATTCAGGATCCATAAAGTTGTTTTTACAGTAGGATAAATTTGAGATAGAGCTGATCTAAGCAGTCGATGATTACAGGGCTAATCGTCCTAAAAGTTACATATCGAGGACCATAGGCTGGGAGCAGTACCCTCATGGTAGATGGAGTGATTCTAGTAATCCTTCATATGGGGCACTAACAGACTATCAGGTATGAATAATACAATAATTGCTCGTAAAATACATTCTGTGTGAATGGTATTTATTAATACATGATCTGTGTGAATGGTATTTGTTATATGGATTCTCTAGAGTATGAGATATAGATAGTGAGAGAGCTGTATGGAGGAAAATCCCTTTTAACCTCTGGAATAAATTCTTGCCGAGTGCCTTTTAGAACTCGAGCCTTGCATTTCCCCTTCAACCTCTATAGTCTATAAATATTGAACTCTGTGATCTGCATGAAATAGAGACTTAAAAGTTTTCTTTGGGAAATTTTTTCCCTCATGATGTAACATTTACTTTTGGCTTGATTAGTCTGACACCATAACATTATCAGAATACACTTCTGCTGAGTCAAATGAAATTTATTGTAGGAAAGTGTATTAATTGTGAAGAAAATCAGTACCAAATCCTTTTGGAAGGTGGTGGTACACTTTTGCCCCTAAATAATGTATTCACTTTTATTGTTTAAATGGGGAGCCGGTGAGATGAAATTTTCTTCTTCTCTCTGGTTTTTTTTTCCTTCTTTCAGGTTTCTTTAACACGTAAAAAAGCTGAAAAATATAAATTTTACTTTCAATTTTTATGTGATTATTGTTACAACACTTTTAAGATGGTTTTTATAACATAGAAATGTAATCCATGTTATGTTTGGTTTGTTTTTCGCTTAAGTTCATGCGGCCACGTGCACGTGACAAGAAGCTTCTCGAGGAATGGGCTCTCCCATTGAAAAGTGTAGAGGACGTTTATGAGGTACTGCTCCTATCCAAGAGTGTAAAGAAATTTGCTATCACTGATAGTGATTGACTAGATATGTTGGACTGTTCATATCAGAAATTTATGAAGTACTGCCTGGGAAAGTTGAGAAGCAGCCCGTGGTCTGAATTAGACGGACTTCAGGCAGAGACAAGGATAATAAGTGAACAGCTTGGGAAAATTAACATGAAAGGTTTCCTTTCAATCAACAGCCAACCAGCAGTAAACGGGGAAAGATCTGATTCTCCATCTGTAGGTAAATAGTAATTAATGCATTTATACAAGTAATTTTTTTGCCAGCATAGCTGAGTAATTGTTTGGAAAGTATGCGATTCTAAAACTGAAACTATATAATTGTTTCTCAGGTATTAAAGATATAATTGACGAAAAATAAAAGTAAAATGATATATTATGGGCTAAGTTATCAATTGCTAAATTTTATTTTATTATATTGTATGTTCTTTTTATATAATTATTAATATATTAAACCATGTAGGACCTTTTGATTGGCTAATAACTTCACAAGTCAACGTACAAGAAATAATTAATAGTTTAAATCCTATTTTAGTCCATACTTGTTCCGTCTAGTCCATTCAAATGTAATGTTTTAGTCCCTCAGTTTCCAATGTTCTGTAGAACCTAAATCTATTTGGAAACCAACATTTAAGTTATTAGTTATTACATGTCACTTCAAACACTTATCTATGTAGACACCTATTGATCAAGTAAAACAAAAAGAGGTGTCGATGTCAGGTTTTTATTAAAAAATTAATATCAAGGACTAGATTAGTTTTTTTATCCAAAGTTTAGAGACTCAAATAGAACGTTTGAAAGTTTACGCACTAAAATTGAATAAATATGAAAGCTTAGAGACTAAAATAAAATTTTAATCTTAATGGCTCAATAACACTAAGTTTTTCTTTTCCTTTTTTTTTTTTAATTTAAATTTTTATAATTAATTTTACAAGAAAATTACTATATGACCAAGTAAAACTTAATTTATATGTTAGAAAAGACACACAAAAAGAGAACACTTGGATTTCATTGAAAAATGAAGTATACAAATGAGGGGCAAAAGCCAAACGATTGCATAAAATAAAGACTTTCTAATTGTCTATAATGGAGGAAAAATGAGTAATTACAGAAAGATTTGGAGAATATAAGATACAATATATTCCAAAGTATGTAAATATTATAATTTTTTTTTTCTTTTAAGCCAAAAATAAAAGTATATTCTGAAGTATTTTTAATGTAATTCTACATATAAACTTTATTTTTTTTTATTAATCATGAAATTGTTTGTCTAATTTGTATATGTCAAATTTTTGTATAGTAAATAATGGATTATGTATAATGCATATGATGAGCTATTAATTATTTGGAAAAAAATGGTTTAAATCATGTGATGCATATTATATTTGGATTTCAAGCCATTTAGCACGTTTTGTTTGTGTTTTTCTTATTTGTTGATATCTGAAAAACTATAGATCAGAGAAAACACTGAACAATCTTGGGGACTTTAGCCTTTCAACCAAAATAATTTCATAATGAAGAAATGGTGCTGTCACCATGCATTGAGTGATCTAATAAAGAGTTGCATGAGAACCTCCCCTAGACTTCTAGTGACAATAATTTAGTATCTGGCATCGAGGGAGATAATTTAATATGCCCAAAGGGGGAGAAGCCCACTTCACTTCTTTCCATTCATATTCATCCTGAAAATCAGAAACCAGTATTGGATCTATCACTCAAACTCTATCAATGGTCATTATAGAGATAAACATGTAAACAGTATCCTACCAAAATCCAATTCTTTTGCCCTTGCCCACTTTAGACGTAGTTGGAATACGATATGGTGTGGAACACTTTGTTTCTTTAATTGATGATTAAGAATGTTTTTATGATGGGGTACAAACTTTAACCATAAATAAATAATAATCTAACAATTATGTGTATGTGTGGGATCTTAATTGCCTCCGTATAGGGAAAAGGGGTATCTTCCACTCTGTTAGGTAGGAATGTCTCCCATGGAATTTGCTGCAAAGAAAATTATATTCTTGCTGGAAACTTCTATATTTGGACCCTATTGGTAATTGTAACCCTTTTTTTATATATATATTAATTCCCATTTTTTTAGAAATGATCTAATTCAGTTCTGACTTTTGACATTTTTGGTAATGGCATCAGAATTCTTATTTAGTCATCTGACTAGTGTTGGGATGATTGATAATGATTTACAGGATGGGGTGGATCAGGAGGTTACGTTTATCAGAAGGCATACGTAGAGTTTTTCTGTTCCAGGGAAAAGTTGGATGCTCTTGTTGATAAGTGCAAGGCTCTTCCATCTCTAACCTACATGGCGGTGAATAAGGAAGGCGGTTGGGTATCCAACATCACTCAAAAGGATGTGAATGCTGTAACATGGGGAGTTTTCCCAGCCAAAGAGATCATCCAGCCAACTGTAGTAGATCCTGCCAGCTTTCTCGTGTGGAAAGATGAGGCATTTGAGATTTGGTCGAGGGGATGGGCTTCCTTGTATCCTGAGGGTGACCCATCCCGAAAATTGCTTGAAGAGGTAGGTGCACTTGTTATATCTTTTCAGTGCTTGATCAATGTATGTTTCTGGACAGTTATCAATTATTCTGTATTCGATATTGTTCCCGTGAATTTCCATTCTTATTTTCTGGATCATCTGGCAGTAGAGAAGTACGTGGAGTATTATTAAGTAGGCTTTGAAGAAAAACGTATGGTGTTATTGATAGGCTTAACTAGTAAAGATTAAATGGTGTTTTATCCAAGGTTAATCCTAATATTGCGGTAATTGAATTGGAAAAGTTTTTTTTTTAGTATTATTATTGATGACAAGGCTTTTTACCATTTGTTTCAGGTTCAGAACAGCTTTTACCTGGTGAGCTTGGTGGACAACGATTTCATCAATTCTAACCTCTTTGTTGCTTTTGATGATTACTAA

mRNA sequence

GTAAAACACATCAGGGAGAAATATGGTGACTACTTCGGCATTACTGTAGCTGGCTATCCAGAGGCACATCCGGACGTGATTGGAAGCAATGGCGTGGCGACGCCGGATGGCTACCAGAGCGATCTGGCTTATCTTAAGAGAAAGTTCGATGCTGGGGCTGATCTGATTGTTACCCAACTATTCTATGACACTGACATTTTCCTCAAGTTTGTCAATGATTGTCGTCAAATTGGAATTACTTGTCCGATTGTACCTGGTATTATGCCCATCAATAATTACAAAGGTTTCTTACGCATGACTGGCTTTTGCAAAACGAAGATTCCGGATGAGATCACTGCTGCCTTAGAGCCTATCAAGGACAACGAAGAAGCTGTGAAAGCTTATGGAATTCACCTTGGAACTGAGATGTGCAAGAAGATTTTGGCTCATGGAATCAAGACTCTACATCTTTATACATTGAACATGGAGAAATCAGCATTGGCTATACTAATGAATCTTGGTCTGATTGAAGAGAGCAAAGTTGCAAGATCTTTACCTTGGAGGCGCCCCACAAATGTTTTCCGTATCAAGGAAGATGTCCGCCCAATCTTTTGGGCTAATCGTCCTAAAAGTTACATATCGAGGACCATAGGCTGGGAGCAGTACCCTCATGGTAGATGGAGTGATTCTAGTAATCCTTCATATGGGGCACTAACAGACTATCAGTTCATGCGGCCACGTGCACGTGACAAGAAGCTTCTCGAGGAATGGGCTCTCCCATTGAAAAGTGTAGAGGACGTTTATGAGAAATTTATGAAGTACTGCCTGGGAAAGTTGAGAAGCAGCCCGTGGTCTGAATTAGACGGACTTCAGGCAGAGACAAGGATAATAAGTGAACAGCTTGGGAAAATTAACATGAAAGGTTTCCTTTCAATCAACAGCCAACCAGCAGTAAACGGGGAAAGATCTGATTCTCCATCTGTAGGATGGGGTGGATCAGGAGGTTACGTTTATCAGAAGGCATACGTAGAGTTTTTCTGTTCCAGGGAAAAGTTGGATGCTCTTGTTGATAAGTGCAAGGCTCTTCCATCTCTAACCTACATGGCGGTGAATAAGGAAGGCGGTTGGGTATCCAACATCACTCAAAAGGATGTGAATGCTGTAACATGGGGAGTTTTCCCAGCCAAAGAGATCATCCAGCCAACTGTAGTAGATCCTGCCAGCTTTCTCGTGTGGAAAGATGAGGCATTTGAGATTTGGTCGAGGGGATGGGCTTCCTTGTATCCTGAGGGTGACCCATCCCGAAAATTGCTTGAAGAGGTTCAGAACAGCTTTTACCTGGTGAGCTTGGTGGACAACGATTTCATCAATTCTAACCTCTTTGTTGCTTTTGATGATTACTAA

Coding sequence (CDS)

GTAAAACACATCAGGGAGAAATATGGTGACTACTTCGGCATTACTGTAGCTGGCTATCCAGAGGCACATCCGGACGTGATTGGAAGCAATGGCGTGGCGACGCCGGATGGCTACCAGAGCGATCTGGCTTATCTTAAGAGAAAGTTCGATGCTGGGGCTGATCTGATTGTTACCCAACTATTCTATGACACTGACATTTTCCTCAAGTTTGTCAATGATTGTCGTCAAATTGGAATTACTTGTCCGATTGTACCTGGTATTATGCCCATCAATAATTACAAAGGTTTCTTACGCATGACTGGCTTTTGCAAAACGAAGATTCCGGATGAGATCACTGCTGCCTTAGAGCCTATCAAGGACAACGAAGAAGCTGTGAAAGCTTATGGAATTCACCTTGGAACTGAGATGTGCAAGAAGATTTTGGCTCATGGAATCAAGACTCTACATCTTTATACATTGAACATGGAGAAATCAGCATTGGCTATACTAATGAATCTTGGTCTGATTGAAGAGAGCAAAGTTGCAAGATCTTTACCTTGGAGGCGCCCCACAAATGTTTTCCGTATCAAGGAAGATGTCCGCCCAATCTTTTGGGCTAATCGTCCTAAAAGTTACATATCGAGGACCATAGGCTGGGAGCAGTACCCTCATGGTAGATGGAGTGATTCTAGTAATCCTTCATATGGGGCACTAACAGACTATCAGTTCATGCGGCCACGTGCACGTGACAAGAAGCTTCTCGAGGAATGGGCTCTCCCATTGAAAAGTGTAGAGGACGTTTATGAGAAATTTATGAAGTACTGCCTGGGAAAGTTGAGAAGCAGCCCGTGGTCTGAATTAGACGGACTTCAGGCAGAGACAAGGATAATAAGTGAACAGCTTGGGAAAATTAACATGAAAGGTTTCCTTTCAATCAACAGCCAACCAGCAGTAAACGGGGAAAGATCTGATTCTCCATCTGTAGGATGGGGTGGATCAGGAGGTTACGTTTATCAGAAGGCATACGTAGAGTTTTTCTGTTCCAGGGAAAAGTTGGATGCTCTTGTTGATAAGTGCAAGGCTCTTCCATCTCTAACCTACATGGCGGTGAATAAGGAAGGCGGTTGGGTATCCAACATCACTCAAAAGGATGTGAATGCTGTAACATGGGGAGTTTTCCCAGCCAAAGAGATCATCCAGCCAACTGTAGTAGATCCTGCCAGCTTTCTCGTGTGGAAAGATGAGGCATTTGAGATTTGGTCGAGGGGATGGGCTTCCTTGTATCCTGAGGGTGACCCATCCCGAAAATTGCTTGAAGAGGTTCAGAACAGCTTTTACCTGGTGAGCTTGGTGGACAACGATTTCATCAATTCTAACCTCTTTGTTGCTTTTGATGATTACTAA

Protein sequence

VKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATPDGYQSDLAYLKRKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCKTKIPDEITAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHLYTLNMEKSALAILMNLGLIEESKVARSLPWRRPTNVFRIKEDVRPIFWANRPKSYISRTIGWEQYPHGRWSDSSNPSYGALTDYQFMRPRARDKKLLEEWALPLKSVEDVYEKFMKYCLGKLRSSPWSELDGLQAETRIISEQLGKINMKGFLSINSQPAVNGERSDSPSVGWGGSGGYVYQKAYVEFFCSREKLDALVDKCKALPSLTYMAVNKEGGWVSNITQKDVNAVTWGVFPAKEIIQPTVVDPASFLVWKDEAFEIWSRGWASLYPEGDPSRKLLEEVQNSFYLVSLVDNDFINSNLFVAFDDY

Homology

[Close]

BLAST of Sgr015688 vs. NCBI nr
Match: XP_038874836.1 (methylenetetrahydrofolate reductase 2-like [Benincasa hispida] >XP_038874837.1 methylenetetrahydrofolate reductase 2-like [Benincasa hispida])

HSP 1 Score: 920.2 bits (2377), Expect = 6.9e-264
Identity = 431/460 (93.70%), Postives = 454/460 (98.70%), Query Frame = 0


Query: 1   VKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATPDGYQSDLAYLKRKFDAGADLIVTQL 60
           VKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATP+GYQSDLAYLK+KFDAGADLIVTQL
Sbjct: 137 VKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATPEGYQSDLAYLKKKFDAGADLIVTQL 196

Query: 61  FYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCKTKIPDEITAALEPIKD 120
           FYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCKTKIPDEI AALEPIKD
Sbjct: 197 FYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCKTKIPDEIMAALEPIKD 256

Query: 121 NEEAVKAYGIHLGTEMCKKILAHGIKTLHLYTLNMEKSALAILMNLGLIEESKVARSLPW 180
           NEEAVKAYGIHLGTEMCKKILAHGIKTLH+YTLNMEKSALAILMNLGLIEESK++RSLPW
Sbjct: 257 NEEAVKAYGIHLGTEMCKKILAHGIKTLHIYTLNMEKSALAILMNLGLIEESKISRSLPW 316

Query: 181 RRPTNVFRIKEDVRPIFWANRPKSYISRTIGWEQYPHGRWSDSSNPSYGALTDYQFMRPR 240
           RRPTNVFR++EDVRPIFWANRP+SYISRTIGW+QYPHGRWSDSSNPSYGALTDYQFMRPR
Sbjct: 317 RRPTNVFRVREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSSNPSYGALTDYQFMRPR 376

Query: 241 ARDKKLLEEWALPLKSVEDVYEKFMKYCLGKLRSSPWSELDGLQAETRIISEQLGKINMK 300
           ARDKKLLEEWALPLKSVEDVYEKFMKYCLGKLRSSPWSEL+GLQAETRIISEQLGK+NMK
Sbjct: 377 ARDKKLLEEWALPLKSVEDVYEKFMKYCLGKLRSSPWSELEGLQAETRIISEQLGKLNMK 436

Query: 301 GFLSINSQPAVNGERSDSPSVGWGGSGGYVYQKAYVEFFCSREKLDALVDKCKALPSLTY 360
           GFLSINSQPAVNGERSDSPSVGWGG GGYVYQKAY+EFFCSREKLD +VDKC++LPSLTY
Sbjct: 437 GFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAYIEFFCSREKLDVVVDKCRSLPSLTY 496

Query: 361 MAVNKEGGWVSNITQKDVNAVTWGVFPAKEIIQPTVVDPASFLVWKDEAFEIWSRGWASL 420
           MAVNKEG WVSNITQKDVNAVTWGVFPAKEIIQPTVVDPASFLVWKDEAFEIWSRGWA+L
Sbjct: 497 MAVNKEGIWVSNITQKDVNAVTWGVFPAKEIIQPTVVDPASFLVWKDEAFEIWSRGWAAL 556

Query: 421 YPEGDPSRKLLEEVQNSFYLVSLVDNDFINSNLFVAFDDY 461
           YPEGDPSRKLLEE+Q+S+YLVSLVDND++NSNLF A +D+
Sbjct: 557 YPEGDPSRKLLEEIQSSYYLVSLVDNDYVNSNLFAALEDF 596

[Close]

BLAST of Sgr015688 vs. NCBI nr
Match: XP_022155988.1 (methylenetetrahydrofolate reductase 2-like [Momordica charantia])

HSP 1 Score: 912.1 bits (2356), Expect = 1.9e-261
Identity = 433/460 (94.13%), Postives = 449/460 (97.61%), Query Frame = 0


Query: 1   VKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATPDGYQSDLAYLKRKFDAGADLIVTQL 60
           VKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATP+GYQSDLAYLK+KFDAGADLIVTQL
Sbjct: 137 VKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATPEGYQSDLAYLKKKFDAGADLIVTQL 196

Query: 61  FYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCKTKIPDEITAALEPIKD 120
           FYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCKTKIPDEI AALEPIKD
Sbjct: 197 FYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCKTKIPDEIMAALEPIKD 256

Query: 121 NEEAVKAYGIHLGTEMCKKILAHGIKTLHLYTLNMEKSALAILMNLGLIEESKVARSLPW 180
           NEEAVKAYGIHLGTEMCKKILAHGIKTLH+YTLNMEKSALAILMNLGLIEESKVARSLPW
Sbjct: 257 NEEAVKAYGIHLGTEMCKKILAHGIKTLHVYTLNMEKSALAILMNLGLIEESKVARSLPW 316

Query: 181 RRPTNVFRIKEDVRPIFWANRPKSYISRTIGWEQYPHGRWSDSSNPSYGALTDYQFMRPR 240
           RRPTNVFRI+EDVRPIFWANRPKSYISRTIGW+QYPHGRWSDSSNPSYGALTDYQFMRPR
Sbjct: 317 RRPTNVFRIREDVRPIFWANRPKSYISRTIGWDQYPHGRWSDSSNPSYGALTDYQFMRPR 376

Query: 241 ARDKKLLEEWALPLKSVEDVYEKFMKYCLGKLRSSPWSELDGLQAETRIISEQLGKINMK 300
           ARDKKLLEEWALPLKS+EDVYEKFMKYCLGKLRSSPWSELDGLQAETRIISEQLGKINMK
Sbjct: 377 ARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELDGLQAETRIISEQLGKINMK 436

Query: 301 GFLSINSQPAVNGERSDSPSVGWGGSGGYVYQKAYVEFFCSREKLDALVDKCKALPSLTY 360
           GFLSINSQPAVNGERSDSPSVGWGG GGYVYQKAY+EFFCSRE LDALVDKC+ LP+LTY
Sbjct: 437 GFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAYLEFFCSRENLDALVDKCRVLPNLTY 496

Query: 361 MAVNKEGGWVSNITQKDVNAVTWGVFPAKEIIQPTVVDPASFLVWKDEAFEIWSRGWASL 420
           MAVNKEG WVSNITQKDVNAVTWGVFPAKEIIQPTVVDPASF VWKDEAFEIW RGWASL
Sbjct: 497 MAVNKEGIWVSNITQKDVNAVTWGVFPAKEIIQPTVVDPASFHVWKDEAFEIWLRGWASL 556

Query: 421 YPEGDPSRKLLEEVQNSFYLVSLVDNDFINSNLFVAFDDY 461
           YPEGD SRKLLEEVQ+S+YLVSLVDND+++SNLF   +D+
Sbjct: 557 YPEGDLSRKLLEEVQSSYYLVSLVDNDYVHSNLFAILEDF 596

[Close]

BLAST of Sgr015688 vs. NCBI nr
Match: XP_023550795.1 (methylenetetrahydrofolate reductase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 910.2 bits (2351), Expect = 7.1e-261
Identity = 429/460 (93.26%), Postives = 448/460 (97.39%), Query Frame = 0


Query: 1   VKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATPDGYQSDLAYLKRKFDAGADLIVTQL 60
           VKHIREKYGDYFGITVAGYPEAHPDVIGSNGVAT +GYQSDLAYLK+KFDAGADLIVTQL
Sbjct: 137 VKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAYLKKKFDAGADLIVTQL 196

Query: 61  FYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCKTKIPDEITAALEPIKD 120
           FYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMT FCKTKIPDE+ AALEPIKD
Sbjct: 197 FYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTSFCKTKIPDEVMAALEPIKD 256

Query: 121 NEEAVKAYGIHLGTEMCKKILAHGIKTLHLYTLNMEKSALAILMNLGLIEESKVARSLPW 180
           NEEAVKAYGIHLGTEMCKKILAHGIKTLH+YTLNMEKSALAILMNLGLIEESKV+RSLPW
Sbjct: 257 NEEAVKAYGIHLGTEMCKKILAHGIKTLHMYTLNMEKSALAILMNLGLIEESKVSRSLPW 316

Query: 181 RRPTNVFRIKEDVRPIFWANRPKSYISRTIGWEQYPHGRWSDSSNPSYGALTDYQFMRPR 240
           RRPTNVFRI+EDVRPIFWANRP+SYISRTIGWEQYPHGRWSDSSNPSYGALTDYQFMRPR
Sbjct: 317 RRPTNVFRIREDVRPIFWANRPRSYISRTIGWEQYPHGRWSDSSNPSYGALTDYQFMRPR 376

Query: 241 ARDKKLLEEWALPLKSVEDVYEKFMKYCLGKLRSSPWSELDGLQAETRIISEQLGKINMK 300
           ARDKKLLEEWAL LKS+EDVYEKFMKYCLGKLRSSPWSELDGLQAETRIISEQLGKINMK
Sbjct: 377 ARDKKLLEEWALSLKSLEDVYEKFMKYCLGKLRSSPWSELDGLQAETRIISEQLGKINMK 436

Query: 301 GFLSINSQPAVNGERSDSPSVGWGGSGGYVYQKAYVEFFCSREKLDALVDKCKALPSLTY 360
           GFLSINSQPAVNGERSDSPSVGWGGSGGYVYQKAY+EFFCSRE LDA+VDKC+ LPSLTY
Sbjct: 437 GFLSINSQPAVNGERSDSPSVGWGGSGGYVYQKAYIEFFCSRENLDAVVDKCRGLPSLTY 496

Query: 361 MAVNKEGGWVSNITQKDVNAVTWGVFPAKEIIQPTVVDPASFLVWKDEAFEIWSRGWASL 420
           MAVNKEG WVSNITQ DVNAVTWGVFPAKEI+QPTVVDPASFLVWKDEAFEIWSRGWASL
Sbjct: 497 MAVNKEGIWVSNITQNDVNAVTWGVFPAKEIVQPTVVDPASFLVWKDEAFEIWSRGWASL 556

Query: 421 YPEGDPSRKLLEEVQNSFYLVSLVDNDFINSNLFVAFDDY 461
           YPEGDPSRK LEE+Q+S+YLVSLVDND++NSNLF A + +
Sbjct: 557 YPEGDPSRKFLEEIQSSYYLVSLVDNDYVNSNLFAALEGF 596

[Close]

BLAST of Sgr015688 vs. NCBI nr
Match: XP_022928798.1 (methylenetetrahydrofolate reductase 2-like [Cucurbita moschata])

HSP 1 Score: 909.8 bits (2350), Expect = 9.3e-261
Identity = 427/460 (92.83%), Postives = 449/460 (97.61%), Query Frame = 0


Query: 1   VKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATPDGYQSDLAYLKRKFDAGADLIVTQL 60
           VKHIREKYGDYFGITVAGYPEAHPDVIGSNGVAT +GYQSDLAYLK+KFDAGADLIVTQL
Sbjct: 137 VKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATAEGYQSDLAYLKKKFDAGADLIVTQL 196

Query: 61  FYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCKTKIPDEITAALEPIKD 120
           FYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMT FCKTKIPDE+ AALEPIKD
Sbjct: 197 FYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTSFCKTKIPDEVMAALEPIKD 256

Query: 121 NEEAVKAYGIHLGTEMCKKILAHGIKTLHLYTLNMEKSALAILMNLGLIEESKVARSLPW 180
           NEEAVKAYGIHLGTEMCKKILAHGIKTLH+YTLNMEKSALAILMNLGLIEESKV+RSLPW
Sbjct: 257 NEEAVKAYGIHLGTEMCKKILAHGIKTLHMYTLNMEKSALAILMNLGLIEESKVSRSLPW 316

Query: 181 RRPTNVFRIKEDVRPIFWANRPKSYISRTIGWEQYPHGRWSDSSNPSYGALTDYQFMRPR 240
           RRPTNVFRI+EDVRPIFWANRP+SYISRTIGW+QYPHGRWSDSSNPSYGALTDYQFMRPR
Sbjct: 317 RRPTNVFRIREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSSNPSYGALTDYQFMRPR 376

Query: 241 ARDKKLLEEWALPLKSVEDVYEKFMKYCLGKLRSSPWSELDGLQAETRIISEQLGKINMK 300
           ARDKKLLEEWALPLKS+EDVYEKFMKYCLGKLRSSPWSELDGLQAETRIISEQLGKINMK
Sbjct: 377 ARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELDGLQAETRIISEQLGKINMK 436

Query: 301 GFLSINSQPAVNGERSDSPSVGWGGSGGYVYQKAYVEFFCSREKLDALVDKCKALPSLTY 360
           GFLSINSQPAVNGE+SDSPSVGWGGSGGYVYQKAY+EFFCSRE LDA+VDKC+ LPSLTY
Sbjct: 437 GFLSINSQPAVNGEKSDSPSVGWGGSGGYVYQKAYIEFFCSRENLDAVVDKCRGLPSLTY 496

Query: 361 MAVNKEGGWVSNITQKDVNAVTWGVFPAKEIIQPTVVDPASFLVWKDEAFEIWSRGWASL 420
           MAVNKEG WVSNITQ DVNAVTWGVFPAKEI+QPTVVDPASFLVWKDEAFEIWSRGWASL
Sbjct: 497 MAVNKEGIWVSNITQNDVNAVTWGVFPAKEIVQPTVVDPASFLVWKDEAFEIWSRGWASL 556

Query: 421 YPEGDPSRKLLEEVQNSFYLVSLVDNDFINSNLFVAFDDY 461
           YPEGDPSRK LEE+Q+S+YLVSLVDN+++NSNLF A + +
Sbjct: 557 YPEGDPSRKFLEEIQSSYYLVSLVDNNYVNSNLFAALEGF 596

[Close]

BLAST of Sgr015688 vs. NCBI nr
Match: XP_022970234.1 (probable methylenetetrahydrofolate reductase [Cucurbita maxima])

HSP 1 Score: 909.1 bits (2348), Expect = 1.6e-260
Identity = 428/460 (93.04%), Postives = 448/460 (97.39%), Query Frame = 0


Query: 1   VKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATPDGYQSDLAYLKRKFDAGADLIVTQL 60
           VKHIREKYGDYFGITVAGYPEAHPDVIGSNGVAT +GYQSDLAYLK+KFDAGADLIVTQL
Sbjct: 137 VKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAYLKKKFDAGADLIVTQL 196

Query: 61  FYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCKTKIPDEITAALEPIKD 120
           FYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMT FCKTKIPDE+ AALEPIKD
Sbjct: 197 FYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTSFCKTKIPDEVMAALEPIKD 256

Query: 121 NEEAVKAYGIHLGTEMCKKILAHGIKTLHLYTLNMEKSALAILMNLGLIEESKVARSLPW 180
           NEEAVKAYGIHLGTEMCKKILAHGIKTLH+YTLNMEKSALAILMNLGLIEESKV+RSLPW
Sbjct: 257 NEEAVKAYGIHLGTEMCKKILAHGIKTLHMYTLNMEKSALAILMNLGLIEESKVSRSLPW 316

Query: 181 RRPTNVFRIKEDVRPIFWANRPKSYISRTIGWEQYPHGRWSDSSNPSYGALTDYQFMRPR 240
           RRPTNVFRI+EDVRPIFWANRP+SYISRTIGW+QYPHGRWSDSSNPSYGALTDYQFMRPR
Sbjct: 317 RRPTNVFRIREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSSNPSYGALTDYQFMRPR 376

Query: 241 ARDKKLLEEWALPLKSVEDVYEKFMKYCLGKLRSSPWSELDGLQAETRIISEQLGKINMK 300
           ARDKKLLEEWALPLKS+EDVYEKFMKYCLGKLRSSPWSELDGLQAETRIISEQLGKINMK
Sbjct: 377 ARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELDGLQAETRIISEQLGKINMK 436

Query: 301 GFLSINSQPAVNGERSDSPSVGWGGSGGYVYQKAYVEFFCSREKLDALVDKCKALPSLTY 360
           GFLSINSQPAVNGERSDSPSVGWGGSGGYVYQKAY+EFFCSRE LDA+VDKC+ LPSLTY
Sbjct: 437 GFLSINSQPAVNGERSDSPSVGWGGSGGYVYQKAYIEFFCSRENLDAVVDKCRGLPSLTY 496

Query: 361 MAVNKEGGWVSNITQKDVNAVTWGVFPAKEIIQPTVVDPASFLVWKDEAFEIWSRGWASL 420
           MAVNKEG WVSNITQ DVNAVTWGVFPAKEI+QPTVVDPASFLVWKDEAFEIWSRGWASL
Sbjct: 497 MAVNKEGIWVSNITQNDVNAVTWGVFPAKEIVQPTVVDPASFLVWKDEAFEIWSRGWASL 556

Query: 421 YPEGDPSRKLLEEVQNSFYLVSLVDNDFINSNLFVAFDDY 461
           YPEGD SRK LEE+Q+S+YLVSLVDND++NSNLF A + +
Sbjct: 557 YPEGDLSRKFLEEIQSSYYLVSLVDNDYVNSNLFAALEGF 596

[Close]

BLAST of Sgr015688 vs. ExPASy Swiss-Prot
Match: O80585 (Methylenetetrahydrofolate reductase 2 OS=Arabidopsis thaliana OX=3702 GN=MTHFR2 PE=1 SV=2)

HSP 1 Score: 802.0 bits (2070), Expect = 3.6e-231
Identity = 373/459 (81.26%), Postives = 414/459 (90.20%), Query Frame = 0


Query: 1   VKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATPDGYQSDLAYLKRKFDAGADLIVTQL 60
           V HIR KYGDYFGITVAGYPEAHPDVIG NG+A+ + YQSDL YLK+K DAGADLIVTQL
Sbjct: 135 VNHIRSKYGDYFGITVAGYPEAHPDVIGENGLASNEAYQSDLEYLKKKIDAGADLIVTQL 194

Query: 61  FYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCKTKIPDEITAALEPIKD 120
           FYDTDIFLKFVNDCRQIGI+CPIVPGIMPINNY+GFLRMTGFCKTKIP E+ AALEPIKD
Sbjct: 195 FYDTDIFLKFVNDCRQIGISCPIVPGIMPINNYRGFLRMTGFCKTKIPVEVMAALEPIKD 254

Query: 121 NEEAVKAYGIHLGTEMCKKILAHGIKTLHLYTLNMEKSALAILMNLGLIEESKVARSLPW 180
           NEEAVKAYGIHLGTEMCKK+LAHG+K+LHLYTLNMEKSALAILMNLG+I+ESK++RSLPW
Sbjct: 255 NEEAVKAYGIHLGTEMCKKMLAHGVKSLHLYTLNMEKSALAILMNLGMIDESKISRSLPW 314

Query: 181 RRPTNVFRIKEDVRPIFWANRPKSYISRTIGWEQYPHGRWSDSSNPSYGALTDYQFMRPR 240
           RRP NVFR KEDVRPIFWANRPKSYISRT GWE +P GRW DS + SYGAL+D+QF RPR
Sbjct: 315 RRPANVFRTKEDVRPIFWANRPKSYISRTKGWEDFPQGRWGDSRSASYGALSDHQFSRPR 374

Query: 241 ARDKKLLEEWALPLKSVEDVYEKFMKYCLGKLRSSPWSELDGLQAETRIISEQLGKINMK 300
           ARDKKL +EW +PLKSVED+ EKF + CLG L+SSPWSELDGLQ ETRII+EQL K+N K
Sbjct: 375 ARDKKLQQEWVVPLKSVEDIQEKFKELCLGNLKSSPWSELDGLQPETRIINEQLIKVNSK 434

Query: 301 GFLSINSQPAVNGERSDSPSVGWGGSGGYVYQKAYVEFFCSREKLDALVDKCKALPSLTY 360
           GFL+INSQP+VN ERSDSP+VGWGG  GYVYQKAY+EFFCS+EKLDA+V+KCKALPS+TY
Sbjct: 435 GFLTINSQPSVNAERSDSPTVGWGGPVGYVYQKAYLEFFCSKEKLDAVVEKCKALPSITY 494

Query: 361 MAVNKEGGWVSNITQKDVNAVTWGVFPAKEIIQPTVVDPASFLVWKDEAFEIWSRGWASL 420
           MAVNK   WVSN  Q DVNAVTWGVFPAKEIIQPT+VDPASF VWKDEAFE WSR WA+L
Sbjct: 495 MAVNKGEQWVSNTAQADVNAVTWGVFPAKEIIQPTIVDPASFNVWKDEAFETWSRSWANL 554

Query: 421 YPEGDPSRKLLEEVQNSFYLVSLVDNDFINSNLFVAFDD 460
           YPE DPSR LLEEV+NS+YLVSLV+ND+IN ++F  F D
Sbjct: 555 YPEADPSRNLLEEVKNSYYLVSLVENDYINGDIFAVFAD 593

[Close]

BLAST of Sgr015688 vs. ExPASy Swiss-Prot
Match: Q75HE6 (Probable methylenetetrahydrofolate reductase OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0815200 PE=2 SV=1)

HSP 1 Score: 798.9 bits (2062), Expect = 3.0e-230
Identity = 375/459 (81.70%), Postives = 412/459 (89.76%), Query Frame = 0


Query: 1   VKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATPDGYQSDLAYLKRKFDAGADLIVTQL 60
           V+HIR KYGDYFGITVAGYPEAHPD I S   ATP+ Y +DLAYLK+K DAGADLI+TQL
Sbjct: 135 VQHIRAKYGDYFGITVAGYPEAHPDAIQSTEGATPEAYSNDLAYLKQKVDAGADLIITQL 194

Query: 61  FYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCKTKIPDEITAALEPIKD 120
           FYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCKTKIP EITAALEPIKD
Sbjct: 195 FYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCKTKIPAEITAALEPIKD 254

Query: 121 NEEAVKAYGIHLGTEMCKKILAHGIKTLHLYTLNMEKSALAILMNLGLIEESKVARSLPW 180
           NEEAVKAYGIHLGTEMCKKILA GIKTLHLYTLNMEKSAL ILMNLGLIEESK++RSLPW
Sbjct: 255 NEEAVKAYGIHLGTEMCKKILATGIKTLHLYTLNMEKSALGILMNLGLIEESKISRSLPW 314

Query: 181 RRPTNVFRIKEDVRPIFWANRPKSYISRTIGWEQYPHGRWSDSSNPSYGALTDYQFMRPR 240
           R PTNVFR+KEDVRPIFWANRPKSYISRT+GW+QYPHGRW DS NPSYGALTDYQF RPR
Sbjct: 315 RPPTNVFRVKEDVRPIFWANRPKSYISRTLGWDQYPHGRWGDSRNPSYGALTDYQFTRPR 374

Query: 241 ARDKKLLEEWALPLKSVEDVYEKFMKYCLGKLRSSPWSELDGLQAETRIISEQLGKINMK 300
            R KKL EEWA+P+KSVED+ E+FM +C GKL SSPWSELDGLQ ET+II +QL KIN K
Sbjct: 375 GRGKKLQEEWAVPVKSVEDINERFMNFCQGKLTSSPWSELDGLQPETKIIDDQLVKINQK 434

Query: 301 GFLSINSQPAVNGERSDSPSVGWGGSGGYVYQKAYVEFFCSREKLDALVDKCKALPSLTY 360
           GFL+INSQPAVNGERSDS SVGWGG GGYVYQKAY+EFFCS+EKLD L++K KA PSLTY
Sbjct: 435 GFLTINSQPAVNGERSDSTSVGWGGPGGYVYQKAYLEFFCSKEKLDQLIEKSKAFPSLTY 494

Query: 361 MAVNKEGGWVSNITQKDVNAVTWGVFPAKEIIQPTVVDPASFLVWKDEAFEIWSRGWASL 420
           +AVNK+G   SNI    VNAVTWGVFP KEI+QPTVVD ASF+VWKDEAFEIWS+GWA L
Sbjct: 495 IAVNKDGESFSNIPTNAVNAVTWGVFPGKEIVQPTVVDSASFMVWKDEAFEIWSKGWACL 554

Query: 421 YPEGDPSRKLLEEVQNSFYLVSLVDNDFINSNLFVAFDD 460
           +PEGD SR++L++VQ S++LVSLVDND+IN +LF AF +
Sbjct: 555 FPEGDSSREILDKVQKSYFLVSLVDNDYINGDLFAAFKE 593

[Close]

BLAST of Sgr015688 vs. ExPASy Swiss-Prot
Match: Q9SE60 (Methylenetetrahydrofolate reductase 1 OS=Arabidopsis thaliana OX=3702 GN=MTHFR1 PE=1 SV=1)

HSP 1 Score: 782.3 bits (2019), Expect = 2.9e-225
Identity = 358/457 (78.34%), Postives = 406/457 (88.84%), Query Frame = 0


Query: 1   VKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATPDGYQSDLAYLKRKFDAGADLIVTQL 60
           V HIR KYGDYFGITVAGYPEAHPDVI ++G+ATP+ YQSDLAYLK+K DAGADLIVTQL
Sbjct: 135 VNHIRSKYGDYFGITVAGYPEAHPDVIEADGLATPESYQSDLAYLKKKVDAGADLIVTQL 194

Query: 61  FYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCKTKIPDEITAALEPIKD 120
           FYDTDIFLKFVNDCRQIGI CPIVPGIMPI+NYKGFLRM GFCKTKIP E+TAALEPIKD
Sbjct: 195 FYDTDIFLKFVNDCRQIGINCPIVPGIMPISNYKGFLRMAGFCKTKIPAELTAALEPIKD 254

Query: 121 NEEAVKAYGIHLGTEMCKKILAHGIKTLHLYTLNMEKSALAILMNLGLIEESKVARSLPW 180
           N+EAVKAYGIH  TEMCKKILAHGI +LHLYTLN++KSA+ ILMNLGLI+ESK++RSLPW
Sbjct: 255 NDEAVKAYGIHFATEMCKKILAHGITSLHLYTLNVDKSAIGILMNLGLIDESKISRSLPW 314

Query: 181 RRPTNVFRIKEDVRPIFWANRPKSYISRTIGWEQYPHGRWSDSSNPSYGALTDYQFMRPR 240
           RRP NVFR KEDVRPIFWANRPKSYISRT GW  +PHGRW DS + +Y  L+DYQF RP+
Sbjct: 315 RRPANVFRTKEDVRPIFWANRPKSYISRTKGWNDFPHGRWGDSHSAAYSTLSDYQFARPK 374

Query: 241 ARDKKLLEEWALPLKSVEDVYEKFMKYCLGKLRSSPWSELDGLQAETRIISEQLGKINMK 300
            RDKKL +EW +PLKS+EDV EKF + C+G L+SSPWSELDGLQ ET+II+EQLGKIN  
Sbjct: 375 GRDKKLQQEWVVPLKSIEDVQEKFKELCIGNLKSSPWSELDGLQPETKIINEQLGKINSN 434

Query: 301 GFLSINSQPAVNGERSDSPSVGWGGSGGYVYQKAYVEFFCSREKLDALVDKCKALPSLTY 360
           GFL+INSQP+VN  +SDSP++GWGG GGYVYQKAY+EFFCS++KLD LV+K KA PS+TY
Sbjct: 435 GFLTINSQPSVNAAKSDSPAIGWGGPGGYVYQKAYLEFFCSKDKLDTLVEKSKAFPSITY 494

Query: 361 MAVNKEGGWVSNITQKDVNAVTWGVFPAKEIIQPTVVDPASFLVWKDEAFEIWSRGWASL 420
           MAVNK   WVSN  + DVNAVTWGVFPAKE+IQPT+VDPASF VWKDEAFEIWSR WA+L
Sbjct: 495 MAVNKSENWVSNTGESDVNAVTWGVFPAKEVIQPTIVDPASFKVWKDEAFEIWSRSWANL 554

Query: 421 YPEGDPSRKLLEEVQNSFYLVSLVDNDFINSNLFVAF 458
           YPE DPSRKLLEEV+NS+YLVSLVDN++IN ++F  F
Sbjct: 555 YPEDDPSRKLLEEVKNSYYLVSLVDNNYINGDIFSVF 591

[Close]

BLAST of Sgr015688 vs. ExPASy Swiss-Prot
Match: Q9SE94 (Methylenetetrahydrofolate reductase 1 OS=Zea mays OX=4577 PE=2 SV=1)

HSP 1 Score: 778.9 bits (2010), Expect = 3.3e-224
Identity = 363/457 (79.43%), Postives = 404/457 (88.40%), Query Frame = 0


Query: 1   VKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATPDGYQSDLAYLKRKFDAGADLIVTQL 60
           V+HIR KYGDYFGITVAGYPEAHPD I   G AT + Y +DLAYLKRK DAGADLIVTQL
Sbjct: 135 VQHIRAKYGDYFGITVAGYPEAHPDAIQGEGGATLEAYSNDLAYLKRKVDAGADLIVTQL 194

Query: 61  FYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCKTKIPDEITAALEPIKD 120
           FYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCKTKIP EITAAL+PIKD
Sbjct: 195 FYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCKTKIPSEITAALDPIKD 254

Query: 121 NEEAVKAYGIHLGTEMCKKILAHGIKTLHLYTLNMEKSALAILMNLGLIEESKVARSLPW 180
           NEEAV+ YGIHLGTEMCKKILA GIKTLHLYTLNM+KSA+ ILMNLGLIEESKV+R LPW
Sbjct: 255 NEEAVRQYGIHLGTEMCKKILATGIKTLHLYTLNMDKSAIGILMNLGLIEESKVSRPLPW 314

Query: 181 RRPTNVFRIKEDVRPIFWANRPKSYISRTIGWEQYPHGRWSDSSNPSYGALTDYQFMRPR 240
           R  TNVFR+KEDVRPIFWANRPKSY+ RT+GW+QYPHGRW DS NPSYGALTD+QF RPR
Sbjct: 315 RPATNVFRVKEDVRPIFWANRPKSYLKRTLGWDQYPHGRWGDSRNPSYGALTDHQFTRPR 374

Query: 241 ARDKKLLEEWALPLKSVEDVYEKFMKYCLGKLRSSPWSELDGLQAETRIISEQLGKINMK 300
            R KKL EEWA+PLKSVED+ E+F  +C GKL SSPWSELDGLQ ET+II +QL  IN K
Sbjct: 375 GRGKKLQEEWAVPLKSVEDISERFTNFCQGKLTSSPWSELDGLQPETKIIDDQLVNINQK 434

Query: 301 GFLSINSQPAVNGERSDSPSVGWGGSGGYVYQKAYVEFFCSREKLDALVDKCKALPSLTY 360
           GFL+INSQPAVNGE+SDSP+VGWGG GGYVYQKAY+EFFC++EKLD L++K KA PSLTY
Sbjct: 435 GFLTINSQPAVNGEKSDSPTVGWGGPGGYVYQKAYLEFFCAKEKLDQLIEKIKAFPSLTY 494

Query: 361 MAVNKEGGWVSNITQKDVNAVTWGVFPAKEIIQPTVVDPASFLVWKDEAFEIWSRGWASL 420
           +AVNK+G   SNI+   VNAVTWGVFP KEIIQPTVVD ASF+VWKDEAFEIW+RGW  +
Sbjct: 495 IAVNKDGETFSNISPNAVNAVTWGVFPGKEIIQPTVVDHASFMVWKDEAFEIWTRGWGCM 554

Query: 421 YPEGDPSRKLLEEVQNSFYLVSLVDNDFINSNLFVAF 458
           +PEGD SR+LLE+VQ ++YLVSLVDND++  +LF AF
Sbjct: 555 FPEGDSSRELLEKVQKTYYLVSLVDNDYVQGDLFAAF 591

[Close]

BLAST of Sgr015688 vs. ExPASy Swiss-Prot
Match: P42898 (Methylenetetrahydrofolate reductase OS=Homo sapiens OX=9606 GN=MTHFR PE=1 SV=3)

HSP 1 Score: 444.1 bits (1141), Expect = 1.9e-123
Identity = 214/459 (46.62%), Postives = 299/459 (65.14%), Query Frame = 0


Query: 1   VKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATPDGYQSDLAYLKRKFDAGADLIVTQL 60
           VKHIR ++GDYF I VAGYP+ HP+            +++DL +LK K  AGAD I+TQL
Sbjct: 179 VKHIRSEFGDYFDICVAGYPKGHPEA---------GSFEADLKHLKEKVSAGADFIITQL 238

Query: 61  FYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCKTKIPDEITAALEPIKD 120
           F++ D F +FV  C  +GITCPIVPGI PI  Y    ++    K ++P EI   +EPIKD
Sbjct: 239 FFEADTFFRFVKACTDMGITCPIVPGIFPIQGYHSLRQLVKLSKLEVPQEIKDVIEPIKD 298

Query: 121 NEEAVKAYGIHLGTEMCKKILAHG-IKTLHLYTLNMEKSALAILMNLGLIEESKVARSLP 180
           N+ A++ YGI L   +C+++LA G +  LH YTLN E +   +L  LG+  E    R LP
Sbjct: 299 NDAAIRNYGIELAVSLCQELLASGLVPGLHFYTLNREMATTEVLKRLGMWTEDP-RRPLP 358

Query: 181 WRRPTNVFRIKEDVRPIFWANRPKSYISRTIGWEQYPHGRWSDSSNPSYGALTDYQ--FM 240
           W    +  R +EDVRPIFWA+RPKSYI RT  W+++P+GRW +SS+P++G L DY   ++
Sbjct: 359 WALSAHPKRREEDVRPIFWASRPKSYIYRTQEWDEFPNGRWGNSSSPAFGELKDYYLFYL 418

Query: 241 RPRARDKKLLEEWALPLKSVEDVYEKFMKYCLG-------KLRSSPWSELDGLQAETRII 300
           + ++  ++LL+ W   L S E V+E F+ Y  G       K+   PW++ + L AET ++
Sbjct: 419 KSKSPKEELLKMWGEELTSEESVFEVFVLYLSGEPNRNGHKVTCLPWND-EPLAAETSLL 478

Query: 301 SEQLGKINMKGFLSINSQPAVNGERSDSPSVGWGGSGGYVYQKAYVEFFCSREKLDALVD 360
            E+L ++N +G L+INSQP +NG+ S  P VGWG SGGYV+QKAY+EFF SRE  +AL+ 
Sbjct: 479 KEELLRVNRQGILTINSQPNINGKPSSDPIVGWGPSGGYVFQKAYLEFFTSRETAEALLQ 538

Query: 361 KCKALP-SLTYMAVNKEGGWVSNITQKDVNAVTWGVFPAKEIIQPTVVDPASFLVWKDEA 420
             K     + Y  VN +G  ++N  +   NAVTWG+FP +EIIQPTVVDP SF+ WKDEA
Sbjct: 539 VLKKYELRVNYHLVNVKGENITNAPELQPNAVTWGIFPGREIIQPTVVDPVSFMFWKDEA 598

Query: 421 FEIWSRGWASLYPEGDPSRKLLEEVQNSFYLVSLVDNDF 449
           F +W   W  LY E  PSR +++ + ++++LV+LVDNDF
Sbjct: 599 FALWIERWGKLYEEESPSRTIIQYIHDNYFLVNLVDNDF 626

[Close]

BLAST of Sgr015688 vs. ExPASy TrEMBL
Match: A0A6J1DPD3 (Methylenetetrahydrofolate reductase OS=Momordica charantia OX=3673 GN=LOC111022964 PE=3 SV=1)

HSP 1 Score: 912.1 bits (2356), Expect = 9.1e-262
Identity = 433/460 (94.13%), Postives = 449/460 (97.61%), Query Frame = 0


Query: 1   VKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATPDGYQSDLAYLKRKFDAGADLIVTQL 60
           VKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATP+GYQSDLAYLK+KFDAGADLIVTQL
Sbjct: 137 VKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATPEGYQSDLAYLKKKFDAGADLIVTQL 196

Query: 61  FYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCKTKIPDEITAALEPIKD 120
           FYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCKTKIPDEI AALEPIKD
Sbjct: 197 FYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCKTKIPDEIMAALEPIKD 256

Query: 121 NEEAVKAYGIHLGTEMCKKILAHGIKTLHLYTLNMEKSALAILMNLGLIEESKVARSLPW 180
           NEEAVKAYGIHLGTEMCKKILAHGIKTLH+YTLNMEKSALAILMNLGLIEESKVARSLPW
Sbjct: 257 NEEAVKAYGIHLGTEMCKKILAHGIKTLHVYTLNMEKSALAILMNLGLIEESKVARSLPW 316

Query: 181 RRPTNVFRIKEDVRPIFWANRPKSYISRTIGWEQYPHGRWSDSSNPSYGALTDYQFMRPR 240
           RRPTNVFRI+EDVRPIFWANRPKSYISRTIGW+QYPHGRWSDSSNPSYGALTDYQFMRPR
Sbjct: 317 RRPTNVFRIREDVRPIFWANRPKSYISRTIGWDQYPHGRWSDSSNPSYGALTDYQFMRPR 376

Query: 241 ARDKKLLEEWALPLKSVEDVYEKFMKYCLGKLRSSPWSELDGLQAETRIISEQLGKINMK 300
           ARDKKLLEEWALPLKS+EDVYEKFMKYCLGKLRSSPWSELDGLQAETRIISEQLGKINMK
Sbjct: 377 ARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELDGLQAETRIISEQLGKINMK 436

Query: 301 GFLSINSQPAVNGERSDSPSVGWGGSGGYVYQKAYVEFFCSREKLDALVDKCKALPSLTY 360
           GFLSINSQPAVNGERSDSPSVGWGG GGYVYQKAY+EFFCSRE LDALVDKC+ LP+LTY
Sbjct: 437 GFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAYLEFFCSRENLDALVDKCRVLPNLTY 496

Query: 361 MAVNKEGGWVSNITQKDVNAVTWGVFPAKEIIQPTVVDPASFLVWKDEAFEIWSRGWASL 420
           MAVNKEG WVSNITQKDVNAVTWGVFPAKEIIQPTVVDPASF VWKDEAFEIW RGWASL
Sbjct: 497 MAVNKEGIWVSNITQKDVNAVTWGVFPAKEIIQPTVVDPASFHVWKDEAFEIWLRGWASL 556

Query: 421 YPEGDPSRKLLEEVQNSFYLVSLVDNDFINSNLFVAFDDY 461
           YPEGD SRKLLEEVQ+S+YLVSLVDND+++SNLF   +D+
Sbjct: 557 YPEGDLSRKLLEEVQSSYYLVSLVDNDYVHSNLFAILEDF 596

[Close]

BLAST of Sgr015688 vs. ExPASy TrEMBL
Match: A0A6J1EQ45 (Methylenetetrahydrofolate reductase OS=Cucurbita moschata OX=3662 GN=LOC111435611 PE=3 SV=1)

HSP 1 Score: 909.8 bits (2350), Expect = 4.5e-261
Identity = 427/460 (92.83%), Postives = 449/460 (97.61%), Query Frame = 0


Query: 1   VKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATPDGYQSDLAYLKRKFDAGADLIVTQL 60
           VKHIREKYGDYFGITVAGYPEAHPDVIGSNGVAT +GYQSDLAYLK+KFDAGADLIVTQL
Sbjct: 137 VKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATAEGYQSDLAYLKKKFDAGADLIVTQL 196

Query: 61  FYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCKTKIPDEITAALEPIKD 120
           FYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMT FCKTKIPDE+ AALEPIKD
Sbjct: 197 FYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTSFCKTKIPDEVMAALEPIKD 256

Query: 121 NEEAVKAYGIHLGTEMCKKILAHGIKTLHLYTLNMEKSALAILMNLGLIEESKVARSLPW 180
           NEEAVKAYGIHLGTEMCKKILAHGIKTLH+YTLNMEKSALAILMNLGLIEESKV+RSLPW
Sbjct: 257 NEEAVKAYGIHLGTEMCKKILAHGIKTLHMYTLNMEKSALAILMNLGLIEESKVSRSLPW 316

Query: 181 RRPTNVFRIKEDVRPIFWANRPKSYISRTIGWEQYPHGRWSDSSNPSYGALTDYQFMRPR 240
           RRPTNVFRI+EDVRPIFWANRP+SYISRTIGW+QYPHGRWSDSSNPSYGALTDYQFMRPR
Sbjct: 317 RRPTNVFRIREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSSNPSYGALTDYQFMRPR 376

Query: 241 ARDKKLLEEWALPLKSVEDVYEKFMKYCLGKLRSSPWSELDGLQAETRIISEQLGKINMK 300
           ARDKKLLEEWALPLKS+EDVYEKFMKYCLGKLRSSPWSELDGLQAETRIISEQLGKINMK
Sbjct: 377 ARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELDGLQAETRIISEQLGKINMK 436

Query: 301 GFLSINSQPAVNGERSDSPSVGWGGSGGYVYQKAYVEFFCSREKLDALVDKCKALPSLTY 360
           GFLSINSQPAVNGE+SDSPSVGWGGSGGYVYQKAY+EFFCSRE LDA+VDKC+ LPSLTY
Sbjct: 437 GFLSINSQPAVNGEKSDSPSVGWGGSGGYVYQKAYIEFFCSRENLDAVVDKCRGLPSLTY 496

Query: 361 MAVNKEGGWVSNITQKDVNAVTWGVFPAKEIIQPTVVDPASFLVWKDEAFEIWSRGWASL 420
           MAVNKEG WVSNITQ DVNAVTWGVFPAKEI+QPTVVDPASFLVWKDEAFEIWSRGWASL
Sbjct: 497 MAVNKEGIWVSNITQNDVNAVTWGVFPAKEIVQPTVVDPASFLVWKDEAFEIWSRGWASL 556

Query: 421 YPEGDPSRKLLEEVQNSFYLVSLVDNDFINSNLFVAFDDY 461
           YPEGDPSRK LEE+Q+S+YLVSLVDN+++NSNLF A + +
Sbjct: 557 YPEGDPSRKFLEEIQSSYYLVSLVDNNYVNSNLFAALEGF 596

[Close]

BLAST of Sgr015688 vs. ExPASy TrEMBL
Match: A0A6J1I4Y4 (Methylenetetrahydrofolate reductase OS=Cucurbita maxima OX=3661 GN=LOC111469179 PE=3 SV=1)

HSP 1 Score: 909.1 bits (2348), Expect = 7.7e-261
Identity = 428/460 (93.04%), Postives = 448/460 (97.39%), Query Frame = 0


Query: 1   VKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATPDGYQSDLAYLKRKFDAGADLIVTQL 60
           VKHIREKYGDYFGITVAGYPEAHPDVIGSNGVAT +GYQSDLAYLK+KFDAGADLIVTQL
Sbjct: 137 VKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAYLKKKFDAGADLIVTQL 196

Query: 61  FYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCKTKIPDEITAALEPIKD 120
           FYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMT FCKTKIPDE+ AALEPIKD
Sbjct: 197 FYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTSFCKTKIPDEVMAALEPIKD 256

Query: 121 NEEAVKAYGIHLGTEMCKKILAHGIKTLHLYTLNMEKSALAILMNLGLIEESKVARSLPW 180
           NEEAVKAYGIHLGTEMCKKILAHGIKTLH+YTLNMEKSALAILMNLGLIEESKV+RSLPW
Sbjct: 257 NEEAVKAYGIHLGTEMCKKILAHGIKTLHMYTLNMEKSALAILMNLGLIEESKVSRSLPW 316

Query: 181 RRPTNVFRIKEDVRPIFWANRPKSYISRTIGWEQYPHGRWSDSSNPSYGALTDYQFMRPR 240
           RRPTNVFRI+EDVRPIFWANRP+SYISRTIGW+QYPHGRWSDSSNPSYGALTDYQFMRPR
Sbjct: 317 RRPTNVFRIREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSSNPSYGALTDYQFMRPR 376

Query: 241 ARDKKLLEEWALPLKSVEDVYEKFMKYCLGKLRSSPWSELDGLQAETRIISEQLGKINMK 300
           ARDKKLLEEWALPLKS+EDVYEKFMKYCLGKLRSSPWSELDGLQAETRIISEQLGKINMK
Sbjct: 377 ARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELDGLQAETRIISEQLGKINMK 436

Query: 301 GFLSINSQPAVNGERSDSPSVGWGGSGGYVYQKAYVEFFCSREKLDALVDKCKALPSLTY 360
           GFLSINSQPAVNGERSDSPSVGWGGSGGYVYQKAY+EFFCSRE LDA+VDKC+ LPSLTY
Sbjct: 437 GFLSINSQPAVNGERSDSPSVGWGGSGGYVYQKAYIEFFCSRENLDAVVDKCRGLPSLTY 496

Query: 361 MAVNKEGGWVSNITQKDVNAVTWGVFPAKEIIQPTVVDPASFLVWKDEAFEIWSRGWASL 420
           MAVNKEG WVSNITQ DVNAVTWGVFPAKEI+QPTVVDPASFLVWKDEAFEIWSRGWASL
Sbjct: 497 MAVNKEGIWVSNITQNDVNAVTWGVFPAKEIVQPTVVDPASFLVWKDEAFEIWSRGWASL 556

Query: 421 YPEGDPSRKLLEEVQNSFYLVSLVDNDFINSNLFVAFDDY 461
           YPEGD SRK LEE+Q+S+YLVSLVDND++NSNLF A + +
Sbjct: 557 YPEGDLSRKFLEEIQSSYYLVSLVDNDYVNSNLFAALEGF 596

[Close]

BLAST of Sgr015688 vs. ExPASy TrEMBL
Match: A0A5A7V2U8 (Methylenetetrahydrofolate reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold767G00900 PE=3 SV=1)

HSP 1 Score: 908.7 bits (2347), Expect = 1.0e-260
Identity = 427/460 (92.83%), Postives = 451/460 (98.04%), Query Frame = 0


Query: 1   VKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATPDGYQSDLAYLKRKFDAGADLIVTQL 60
           VKHIREKYGDYFGITVAGYPEAHPDVIGSNGVAT +GYQSDLAYLK+KFDAGADLIVTQL
Sbjct: 137 VKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAYLKKKFDAGADLIVTQL 196

Query: 61  FYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCKTKIPDEITAALEPIKD 120
           FYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCKTKIPDEI AALEPIKD
Sbjct: 197 FYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCKTKIPDEIMAALEPIKD 256

Query: 121 NEEAVKAYGIHLGTEMCKKILAHGIKTLHLYTLNMEKSALAILMNLGLIEESKVARSLPW 180
           NEEAVKAYGIHLGTEMCKKILAHGIKTLH+YTLNMEKSALAILMNLGLIEESKV+RSLPW
Sbjct: 257 NEEAVKAYGIHLGTEMCKKILAHGIKTLHIYTLNMEKSALAILMNLGLIEESKVSRSLPW 316

Query: 181 RRPTNVFRIKEDVRPIFWANRPKSYISRTIGWEQYPHGRWSDSSNPSYGALTDYQFMRPR 240
           RRPTNVFR++EDVRPIFWANRP+SYISRTIGW+QYPHGRWSDS NPSYGALTDYQFMRPR
Sbjct: 317 RRPTNVFRVREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSRNPSYGALTDYQFMRPR 376

Query: 241 ARDKKLLEEWALPLKSVEDVYEKFMKYCLGKLRSSPWSELDGLQAETRIISEQLGKINMK 300
           ARDKKLLEEWALPLKS+EDVYEKFMKYCLGKLRSSPWSEL+GLQAETR+ISEQLGKINMK
Sbjct: 377 ARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQAETRMISEQLGKINMK 436

Query: 301 GFLSINSQPAVNGERSDSPSVGWGGSGGYVYQKAYVEFFCSREKLDALVDKCKALPSLTY 360
           GFLSINSQPAVNGERSDSPSVGWGG GGYVYQKAY+EFFCSREKLDA+VDKC+ALPSLTY
Sbjct: 437 GFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAYIEFFCSREKLDAIVDKCRALPSLTY 496

Query: 361 MAVNKEGGWVSNITQKDVNAVTWGVFPAKEIIQPTVVDPASFLVWKDEAFEIWSRGWASL 420
           MAVNKEG WVSN+TQKDVNAVTWGVFPAKEIIQPTVVDPASFLVWKDEAFEIWSRGWA+L
Sbjct: 497 MAVNKEGIWVSNVTQKDVNAVTWGVFPAKEIIQPTVVDPASFLVWKDEAFEIWSRGWAAL 556

Query: 421 YPEGDPSRKLLEEVQNSFYLVSLVDNDFINSNLFVAFDDY 461
           YPEGD SRKLL+E+Q+S+YLVSLVDND+INS+LF   +D+
Sbjct: 557 YPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILEDF 596

[Close]

BLAST of Sgr015688 vs. ExPASy TrEMBL
Match: A0A6J1JBG5 (Methylenetetrahydrofolate reductase OS=Cucurbita maxima OX=3661 GN=LOC111482941 PE=3 SV=1)

HSP 1 Score: 902.9 bits (2332), Expect = 5.5e-259
Identity = 422/460 (91.74%), Postives = 448/460 (97.39%), Query Frame = 0


Query: 1   VKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATPDGYQSDLAYLKRKFDAGADLIVTQL 60
           VKHIREKYGDYFGITVAGYPEAHPDVIGSNGVAT +GYQSDLAYLKRKFDAGADLIVTQL
Sbjct: 137 VKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATLEGYQSDLAYLKRKFDAGADLIVTQL 196

Query: 61  FYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCKTKIPDEITAALEPIKD 120
           FYDTDIFLKFVNDCRQIGITCPI+PGIMPINNYKGF+RMT FCKTK+PDEI AALEPIKD
Sbjct: 197 FYDTDIFLKFVNDCRQIGITCPIIPGIMPINNYKGFIRMTEFCKTKVPDEIMAALEPIKD 256

Query: 121 NEEAVKAYGIHLGTEMCKKILAHGIKTLHLYTLNMEKSALAILMNLGLIEESKVARSLPW 180
           NEEAVKAYGIHLGTEMCKKILAHGIKTLHLYTLNMEKSALAILM+LGLIEESK++RSLPW
Sbjct: 257 NEEAVKAYGIHLGTEMCKKILAHGIKTLHLYTLNMEKSALAILMSLGLIEESKISRSLPW 316

Query: 181 RRPTNVFRIKEDVRPIFWANRPKSYISRTIGWEQYPHGRWSDSSNPSYGALTDYQFMRPR 240
           RRPTN+FR+ EDVRPIFWANRPKSYISRTIGW+QYPHGRW+DS NPSYGALTDYQFMRPR
Sbjct: 317 RRPTNIFRVHEDVRPIFWANRPKSYISRTIGWDQYPHGRWTDSRNPSYGALTDYQFMRPR 376

Query: 241 ARDKKLLEEWALPLKSVEDVYEKFMKYCLGKLRSSPWSELDGLQAETRIISEQLGKINMK 300
           ARDKKLLEEWALPL S+EDVYEKFMKYCLGKLRSSPWSELDGLQAETRIISEQLGKINMK
Sbjct: 377 ARDKKLLEEWALPLNSIEDVYEKFMKYCLGKLRSSPWSELDGLQAETRIISEQLGKINMK 436

Query: 301 GFLSINSQPAVNGERSDSPSVGWGGSGGYVYQKAYVEFFCSREKLDALVDKCKALPSLTY 360
           G L+INSQP+VNGERSDSPSVGWGGSGGYVYQKAY+EFFCS+EKLDA+VDKC+ALPSLTY
Sbjct: 437 GLLTINSQPSVNGERSDSPSVGWGGSGGYVYQKAYIEFFCSKEKLDAVVDKCRALPSLTY 496

Query: 361 MAVNKEGGWVSNITQKDVNAVTWGVFPAKEIIQPTVVDPASFLVWKDEAFEIWSRGWASL 420
           MAVNKEG WVSNITQKDVNAVTWGVFPAKEIIQPTVVDPASF VWKDEAFE+WSRGWASL
Sbjct: 497 MAVNKEGVWVSNITQKDVNAVTWGVFPAKEIIQPTVVDPASFHVWKDEAFELWSRGWASL 556

Query: 421 YPEGDPSRKLLEEVQNSFYLVSLVDNDFINSNLFVAFDDY 461
           YPEGDPSRKLLEE+Q S+YLVSLVDND++NSN+F A +D+
Sbjct: 557 YPEGDPSRKLLEEIQISYYLVSLVDNDYVNSNIFAALEDF 596

[Close]

BLAST of Sgr015688 vs. TAIR 10
Match: AT2G44160.1 (methylenetetrahydrofolate reductase 2 )

HSP 1 Score: 802.0 bits (2070), Expect = 2.5e-232
Identity = 373/459 (81.26%), Postives = 414/459 (90.20%), Query Frame = 0


Query: 1   VKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATPDGYQSDLAYLKRKFDAGADLIVTQL 60
           V HIR KYGDYFGITVAGYPEAHPDVIG NG+A+ + YQSDL YLK+K DAGADLIVTQL
Sbjct: 135 VNHIRSKYGDYFGITVAGYPEAHPDVIGENGLASNEAYQSDLEYLKKKIDAGADLIVTQL 194

Query: 61  FYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCKTKIPDEITAALEPIKD 120
           FYDTDIFLKFVNDCRQIGI+CPIVPGIMPINNY+GFLRMTGFCKTKIP E+ AALEPIKD
Sbjct: 195 FYDTDIFLKFVNDCRQIGISCPIVPGIMPINNYRGFLRMTGFCKTKIPVEVMAALEPIKD 254

Query: 121 NEEAVKAYGIHLGTEMCKKILAHGIKTLHLYTLNMEKSALAILMNLGLIEESKVARSLPW 180
           NEEAVKAYGIHLGTEMCKK+LAHG+K+LHLYTLNMEKSALAILMNLG+I+ESK++RSLPW
Sbjct: 255 NEEAVKAYGIHLGTEMCKKMLAHGVKSLHLYTLNMEKSALAILMNLGMIDESKISRSLPW 314

Query: 181 RRPTNVFRIKEDVRPIFWANRPKSYISRTIGWEQYPHGRWSDSSNPSYGALTDYQFMRPR 240
           RRP NVFR KEDVRPIFWANRPKSYISRT GWE +P GRW DS + SYGAL+D+QF RPR
Sbjct: 315 RRPANVFRTKEDVRPIFWANRPKSYISRTKGWEDFPQGRWGDSRSASYGALSDHQFSRPR 374

Query: 241 ARDKKLLEEWALPLKSVEDVYEKFMKYCLGKLRSSPWSELDGLQAETRIISEQLGKINMK 300
           ARDKKL +EW +PLKSVED+ EKF + CLG L+SSPWSELDGLQ ETRII+EQL K+N K
Sbjct: 375 ARDKKLQQEWVVPLKSVEDIQEKFKELCLGNLKSSPWSELDGLQPETRIINEQLIKVNSK 434

Query: 301 GFLSINSQPAVNGERSDSPSVGWGGSGGYVYQKAYVEFFCSREKLDALVDKCKALPSLTY 360
           GFL+INSQP+VN ERSDSP+VGWGG  GYVYQKAY+EFFCS+EKLDA+V+KCKALPS+TY
Sbjct: 435 GFLTINSQPSVNAERSDSPTVGWGGPVGYVYQKAYLEFFCSKEKLDAVVEKCKALPSITY 494

Query: 361 MAVNKEGGWVSNITQKDVNAVTWGVFPAKEIIQPTVVDPASFLVWKDEAFEIWSRGWASL 420
           MAVNK   WVSN  Q DVNAVTWGVFPAKEIIQPT+VDPASF VWKDEAFE WSR WA+L
Sbjct: 495 MAVNKGEQWVSNTAQADVNAVTWGVFPAKEIIQPTIVDPASFNVWKDEAFETWSRSWANL 554

Query: 421 YPEGDPSRKLLEEVQNSFYLVSLVDNDFINSNLFVAFDD 460
           YPE DPSR LLEEV+NS+YLVSLV+ND+IN ++F  F D
Sbjct: 555 YPEADPSRNLLEEVKNSYYLVSLVENDYINGDIFAVFAD 593

[Close]

BLAST of Sgr015688 vs. TAIR 10
Match: AT3G59970.3 (methylenetetrahydrofolate reductase 1 )

HSP 1 Score: 782.3 bits (2019), Expect = 2.1e-226
Identity = 358/457 (78.34%), Postives = 406/457 (88.84%), Query Frame = 0


Query: 1   VKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATPDGYQSDLAYLKRKFDAGADLIVTQL 60
           V HIR KYGDYFGITVAGYPEAHPDVI ++G+ATP+ YQSDLAYLK+K DAGADLIVTQL
Sbjct: 135 VNHIRSKYGDYFGITVAGYPEAHPDVIEADGLATPESYQSDLAYLKKKVDAGADLIVTQL 194

Query: 61  FYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCKTKIPDEITAALEPIKD 120
           FYDTDIFLKFVNDCRQIGI CPIVPGIMPI+NYKGFLRM GFCKTKIP E+TAALEPIKD
Sbjct: 195 FYDTDIFLKFVNDCRQIGINCPIVPGIMPISNYKGFLRMAGFCKTKIPAELTAALEPIKD 254

Query: 121 NEEAVKAYGIHLGTEMCKKILAHGIKTLHLYTLNMEKSALAILMNLGLIEESKVARSLPW 180
           N+EAVKAYGIH  TEMCKKILAHGI +LHLYTLN++KSA+ ILMNLGLI+ESK++RSLPW
Sbjct: 255 NDEAVKAYGIHFATEMCKKILAHGITSLHLYTLNVDKSAIGILMNLGLIDESKISRSLPW 314

Query: 181 RRPTNVFRIKEDVRPIFWANRPKSYISRTIGWEQYPHGRWSDSSNPSYGALTDYQFMRPR 240
           RRP NVFR KEDVRPIFWANRPKSYISRT GW  +PHGRW DS + +Y  L+DYQF RP+
Sbjct: 315 RRPANVFRTKEDVRPIFWANRPKSYISRTKGWNDFPHGRWGDSHSAAYSTLSDYQFARPK 374

Query: 241 ARDKKLLEEWALPLKSVEDVYEKFMKYCLGKLRSSPWSELDGLQAETRIISEQLGKINMK 300
            RDKKL +EW +PLKS+EDV EKF + C+G L+SSPWSELDGLQ ET+II+EQLGKIN  
Sbjct: 375 GRDKKLQQEWVVPLKSIEDVQEKFKELCIGNLKSSPWSELDGLQPETKIINEQLGKINSN 434

Query: 301 GFLSINSQPAVNGERSDSPSVGWGGSGGYVYQKAYVEFFCSREKLDALVDKCKALPSLTY 360
           GFL+INSQP+VN  +SDSP++GWGG GGYVYQKAY+EFFCS++KLD LV+K KA PS+TY
Sbjct: 435 GFLTINSQPSVNAAKSDSPAIGWGGPGGYVYQKAYLEFFCSKDKLDTLVEKSKAFPSITY 494

Query: 361 MAVNKEGGWVSNITQKDVNAVTWGVFPAKEIIQPTVVDPASFLVWKDEAFEIWSRGWASL 420
           MAVNK   WVSN  + DVNAVTWGVFPAKE+IQPT+VDPASF VWKDEAFEIWSR WA+L
Sbjct: 495 MAVNKSENWVSNTGESDVNAVTWGVFPAKEVIQPTIVDPASFKVWKDEAFEIWSRSWANL 554

Query: 421 YPEGDPSRKLLEEVQNSFYLVSLVDNDFINSNLFVAF 458
           YPE DPSRKLLEEV+NS+YLVSLVDN++IN ++F  F
Sbjct: 555 YPEDDPSRKLLEEVKNSYYLVSLVDNNYINGDIFSVF 591

[Close]

BLAST of Sgr015688 vs. TAIR 10
Match: AT3G59970.2 (methylenetetrahydrofolate reductase 1 )

HSP 1 Score: 463.4 bits (1191), Expect = 2.1e-130
Identity = 212/263 (80.61%), Postives = 236/263 (89.73%), Query Frame = 0


Query: 1   VKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATPDGYQSDLAYLKRKFDAGADLIVTQL 60
           V HIR KYGDYFGITVAGYPEAHPDVI ++G+ATP+ YQSDLAYLK+K DAGADLIVTQL
Sbjct: 135 VNHIRSKYGDYFGITVAGYPEAHPDVIEADGLATPESYQSDLAYLKKKVDAGADLIVTQL 194

Query: 61  FYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCKTKIPDEITAALEPIKD 120
           FYDTDIFLKFVNDCRQIGI CPIVPGIMPI+NYKGFLRM GFCKTKIP E+TAALEPIKD
Sbjct: 195 FYDTDIFLKFVNDCRQIGINCPIVPGIMPISNYKGFLRMAGFCKTKIPAELTAALEPIKD 254

Query: 121 NEEAVKAYGIHLGTEMCKKILAHGIKTLHLYTLNMEKSALAILMNLGLIEESKVARSLPW 180
           N+EAVKAYGIH  TEMCKKILAHGI +LHLYTLN++KSA+ ILMNLGLI+ESK++RSLPW
Sbjct: 255 NDEAVKAYGIHFATEMCKKILAHGITSLHLYTLNVDKSAIGILMNLGLIDESKISRSLPW 314

Query: 181 RRPTNVFRIKEDVRPIFWANRPKSYISRTIGWEQYPHGRWSDSSNPSYGALTDYQFMRPR 240
           RRP NVFR KEDVRPIFWANRPKSYISRT GW  +PHGRW DS + +Y  L+DYQF RP+
Sbjct: 315 RRPANVFRTKEDVRPIFWANRPKSYISRTKGWNDFPHGRWGDSHSAAYSTLSDYQFARPK 374

Query: 241 ARDKKLLEEWALPLKSVEDVYEK 264
            RDKKL +EW +PLKS+EDV E+
Sbjct: 375 GRDKKLQQEWVVPLKSIEDVQER 397

[Close]

BLAST of Sgr015688 vs. TAIR 10
Match: AT3G59970.1 (methylenetetrahydrofolate reductase 1 )

HSP 1 Score: 462.6 bits (1189), Expect = 3.7e-130
Identity = 212/262 (80.92%), Postives = 235/262 (89.69%), Query Frame = 0


Query: 1   VKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATPDGYQSDLAYLKRKFDAGADLIVTQL 60
           V HIR KYGDYFGITVAGYPEAHPDVI ++G+ATP+ YQSDLAYLK+K DAGADLIVTQL
Sbjct: 135 VNHIRSKYGDYFGITVAGYPEAHPDVIEADGLATPESYQSDLAYLKKKVDAGADLIVTQL 194

Query: 61  FYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCKTKIPDEITAALEPIKD 120
           FYDTDIFLKFVNDCRQIGI CPIVPGIMPI+NYKGFLRM GFCKTKIP E+TAALEPIKD
Sbjct: 195 FYDTDIFLKFVNDCRQIGINCPIVPGIMPISNYKGFLRMAGFCKTKIPAELTAALEPIKD 254

Query: 121 NEEAVKAYGIHLGTEMCKKILAHGIKTLHLYTLNMEKSALAILMNLGLIEESKVARSLPW 180
           N+EAVKAYGIH  TEMCKKILAHGI +LHLYTLN++KSA+ ILMNLGLI+ESK++RSLPW
Sbjct: 255 NDEAVKAYGIHFATEMCKKILAHGITSLHLYTLNVDKSAIGILMNLGLIDESKISRSLPW 314

Query: 181 RRPTNVFRIKEDVRPIFWANRPKSYISRTIGWEQYPHGRWSDSSNPSYGALTDYQFMRPR 240
           RRP NVFR KEDVRPIFWANRPKSYISRT GW  +PHGRW DS + +Y  L+DYQF RP+
Sbjct: 315 RRPANVFRTKEDVRPIFWANRPKSYISRTKGWNDFPHGRWGDSHSAAYSTLSDYQFARPK 374

Query: 241 ARDKKLLEEWALPLKSVEDVYE 263
            RDKKL +EW +PLKS+EDV E
Sbjct: 375 GRDKKLQQEWVVPLKSIEDVQE 396

The following BLAST results are available for this feature:

BLAST of Sgr015688 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038874836.1	6.9e-264	93.70	methylenetetrahydrofolate reductase 2-like [Benincasa hispida] >XP_038874837.1 m...	[more]
XP_022155988.1	1.9e-261	94.13	methylenetetrahydrofolate reductase 2-like [Momordica charantia]	[more]
XP_023550795.1	7.1e-261	93.26	methylenetetrahydrofolate reductase 2-like [Cucurbita pepo subsp. pepo]	[more]
XP_022928798.1	9.3e-261	92.83	methylenetetrahydrofolate reductase 2-like [Cucurbita moschata]	[more]
XP_022970234.1	1.6e-260	93.04	probable methylenetetrahydrofolate reductase [Cucurbita maxima]	[more]

BLAST of Sgr015688 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O80585	3.6e-231	81.26	Methylenetetrahydrofolate reductase 2 OS=Arabidopsis thaliana OX=3702 GN=MTHFR2 ...	[more]
Q75HE6	3.0e-230	81.70	Probable methylenetetrahydrofolate reductase OS=Oryza sativa subsp. japonica OX=...	[more]
Q9SE60	2.9e-225	78.34	Methylenetetrahydrofolate reductase 1 OS=Arabidopsis thaliana OX=3702 GN=MTHFR1 ...	[more]
Q9SE94	3.3e-224	79.43	Methylenetetrahydrofolate reductase 1 OS=Zea mays OX=4577 PE=2 SV=1	[more]
P42898	1.9e-123	46.62	Methylenetetrahydrofolate reductase OS=Homo sapiens OX=9606 GN=MTHFR PE=1 SV=3	[more]

BLAST of Sgr015688 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DPD3	9.1e-262	94.13	Methylenetetrahydrofolate reductase OS=Momordica charantia OX=3673 GN=LOC1110229...	[more]
A0A6J1EQ45	4.5e-261	92.83	Methylenetetrahydrofolate reductase OS=Cucurbita moschata OX=3662 GN=LOC11143561...	[more]
A0A6J1I4Y4	7.7e-261	93.04	Methylenetetrahydrofolate reductase OS=Cucurbita maxima OX=3661 GN=LOC111469179 ...	[more]
A0A5A7V2U8	1.0e-260	92.83	Methylenetetrahydrofolate reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5...	[more]
A0A6J1JBG5	5.5e-259	91.74	Methylenetetrahydrofolate reductase OS=Cucurbita maxima OX=3661 GN=LOC111482941 ...	[more]

BLAST of Sgr015688 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G44160.1	2.5e-232	81.26	methylenetetrahydrofolate reductase 2	[more]
AT3G59970.3	2.1e-226	78.34	methylenetetrahydrofolate reductase 1	[more]
AT3G59970.2	2.1e-130	80.61	methylenetetrahydrofolate reductase 1	[more]
AT3G59970.1	3.7e-130	80.92	methylenetetrahydrofolate reductase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.20.20.220		coord: 1..170 e-value: 5.3E-63 score: 214.8
None	No IPR available	PANTHER	PTHR45754:SF4	METHYLENETETRAHYDROFOLATE REDUCTASE 1	coord: 1..457
None	No IPR available	PANTHER	PTHR45754	METHYLENETETRAHYDROFOLATE REDUCTASE	coord: 1..457
IPR003171	Methylenetetrahydrofolate reductase-like	PFAM	PF02219	MTHFR	coord: 1..167 e-value: 2.9E-66 score: 223.7
IPR003171	Methylenetetrahydrofolate reductase-like	CDD	cd00537	MTHFR	coord: 1..166 e-value: 8.02257E-65 score: 208.239
IPR029041	FAD-linked oxidoreductase-like	SUPERFAMILY	51730	FAD-linked oxidoreductase	coord: 1..168

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sgr015688.1	Sgr015688.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009086	methionine biosynthetic process
biological_process	GO:0035999	tetrahydrofolate interconversion
biological_process	GO:0006555	methionine metabolic process
cellular_component	GO:0005829	cytosol
molecular_function	GO:0071949	FAD binding
molecular_function	GO:0106312	methylenetetrahydrofolate reductase NADH activity
molecular_function	GO:0106313	methylenetetrahydrofolate reductase NADPH activity
molecular_function	GO:0004489	methylenetetrahydrofolate reductase (NAD(P)H) activity