Sgr015674 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr015674 vs. NCBI nr
Match: KAG7025832.1 (hypothetical protein SDJN02_12330, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 233.4 bits (594), Expect = 1.5e-57
Identity = 128/187 (68.45%), Postives = 143/187 (76.47%), Query Frame = 0

Query: 1   MENNLPVIAKK----VLNLQSHVPPPLRRRHRLLAAARRSHEIGHLPFDVGADEYEFSCS 60
           MENNLP++AKK      +  +  PPP         ++      G LPF +GADEYEFSCS
Sbjct: 1   MENNLPIMAKKPSATSCSTTTTTPPPPPPP----PSSSPPTSAGQLPFPIGADEYEFSCS 60

Query: 61  NSPAFPGLHFPGFHVGKRRRNQHHNSFFACAQAPETLDDDATAVNAVKAVMEMLNNHGGA 120
           NSPA     FP FHVGKRRRNQ+HNSFFACA AP+TLDDDA AVNAV AV+E+LNNH GA
Sbjct: 61  NSPA-----FPTFHVGKRRRNQNHNSFFACAHAPDTLDDDAAAVNAVNAVVEILNNHTGA 120

Query: 121 SSS-SVQASPTLPGFGRTP-RVRQLRITDSPFPLQDANADPQVDKAADEFISRFYKELRL 180
           SS+  + ASP LPGFG TP RVRQLRITDSPFPLQDANADP VDKAADEFISRFYKELRL
Sbjct: 121 SSTPPIPASPALPGFGPTPRRVRQLRITDSPFPLQDANADPLVDKAADEFISRFYKELRL 178

Query: 181 QKTADDD 182
           QKTAD++
Sbjct: 181 QKTADEN 178

BLAST of Sgr015674 vs. NCBI nr
Match: XP_023000392.1 (uncharacterized protein LOC111494650 [Cucurbita maxima])

HSP 1 Score: 231.1 bits (588), Expect = 7.6e-57
Identity = 137/218 (62.84%), Postives = 148/218 (67.89%), Query Frame = 0

Query: 1   MENNLPVIAKKVLNLQSHVPPPLRR------------------------------RHRLL 60
           MENNLP++AKKV NL       LR+                               H   
Sbjct: 1   MENNLPIMAKKVWNLVRVAYFLLRKGISKSKLMLDLNLMTKRGKLAGKAISNLMFHHHYH 60

Query: 61  AAARRSH----EIGHLPFDVGADEYEFSCSNSPAFPGLHFPGFHVGKRRRNQHHNSFFAC 120
           AAA  S       G LPF +GADEYEFSCSNSPA     FP FHVGKRRRNQ+HNSFFAC
Sbjct: 61  AAASPSSSPPTSAGQLPFPIGADEYEFSCSNSPA-----FPAFHVGKRRRNQNHNSFFAC 120

Query: 121 AQAPETLDDD-ATAVNAVKAVMEMLNNHGGASSS-SVQASPTLPGFGRTP-RVRQLRITD 180
           A AP+TLDDD A AVNAV AV+E+LNNH GASS+  V ASP LPGFGRTP RVRQLRITD
Sbjct: 121 AHAPDTLDDDAAAAVNAVNAVVEILNNHSGASSTPPVPASPALPGFGRTPRRVRQLRITD 180

Query: 181 SPFPLQDANADPQVDKAADEFISRFYKELRLQKTADDD 182
           SPFPLQDANADP VDKAADEFISRFYKELRLQKTAD++
Sbjct: 181 SPFPLQDANADPLVDKAADEFISRFYKELRLQKTADEN 213

BLAST of Sgr015674 vs. NCBI nr
Match: XP_023514516.1 (uncharacterized protein LOC111778773 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 226.5 bits (576), Expect = 1.9e-55
Identity = 124/178 (69.66%), Postives = 135/178 (75.84%), Query Frame = 0

Query: 10  KKVLNLQSHVPPPLRRRHRLLAAARRSH----EIGHLPFDVGADEYEFSCSNSPAFPGLH 69
           K + NL  H        H   AA+  S       G LPF +GADEYEFSCSNSPA     
Sbjct: 41  KAISNLMFH-----HHYHAAAAASPSSSSPPTSTGQLPFPIGADEYEFSCSNSPA----- 100

Query: 70  FPGFHVGKRRRNQHHNSFFACAQAPETLDDDATAVNAVKAVMEMLNNHGGASSS-SVQAS 129
           FP FHVGKRRRNQ+HN FFACA AP+TLDDDA AVNAV AV+E+LNNH GASS+  + AS
Sbjct: 101 FPAFHVGKRRRNQNHNPFFACAHAPDTLDDDAAAVNAVNAVVEILNNHTGASSTPPIPAS 160

Query: 130 PTLPGFGRTP-RVRQLRITDSPFPLQDANADPQVDKAADEFISRFYKELRLQKTADDD 182
           P LPGFGRTP RVRQLRITDSPFPLQDANADP VDKAADEFISRFYKELRLQKTAD++
Sbjct: 161 PALPGFGRTPRRVRQLRITDSPFPLQDANADPLVDKAADEFISRFYKELRLQKTADEN 208

BLAST of Sgr015674 vs. NCBI nr
Match: XP_022964083.1 (uncharacterized protein LOC111464220 [Cucurbita moschata])

HSP 1 Score: 226.5 bits (576), Expect = 1.9e-55
Identity = 126/178 (70.79%), Postives = 135/178 (75.84%), Query Frame = 0

Query: 10  KKVLNLQSHVPPPLRRRHRLLAAARRSH----EIGHLPFDVGADEYEFSCSNSPAFPGLH 69
           K + NL  H        H   AAA  S       G LPF VGADEYEFSCSNSPA     
Sbjct: 41  KAISNLMFH-------HHYHAAAASPSSSPPTSAGQLPFPVGADEYEFSCSNSPA----- 100

Query: 70  FPGFHVGKRRRNQHHNSFFACAQAPETLDDDATAVNAVKAVMEMLNNHGGASSS-SVQAS 129
           FP FHVGKRRRNQ+HNSFFACA AP+TLDDDA AVNAV AV+E+LNNH GASS+  + AS
Sbjct: 101 FPAFHVGKRRRNQNHNSFFACAHAPDTLDDDAAAVNAVNAVVEILNNHTGASSTPPIPAS 160

Query: 130 PTLPGFGRTP-RVRQLRITDSPFPLQDANADPQVDKAADEFISRFYKELRLQKTADDD 182
           P LPGFG TP RVRQLRITDSPFPLQDANADP VDKAADEFISRFYKELRLQKTAD++
Sbjct: 161 PALPGFGPTPRRVRQLRITDSPFPLQDANADPLVDKAADEFISRFYKELRLQKTADEN 206

BLAST of Sgr015674 vs. NCBI nr
Match: KAG6593486.1 (hypothetical protein SDJN03_12962, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 225.7 bits (574), Expect = 3.2e-55
Identity = 125/179 (69.83%), Postives = 135/179 (75.42%), Query Frame = 0

Query: 10  KKVLNLQSHVPPPLRRRHRLLAAARRSH-----EIGHLPFDVGADEYEFSCSNSPAFPGL 69
           K + NL  H        H   AAA  S        G LPF +GADEYEFSCSNSPA    
Sbjct: 16  KAISNLMFH-----HHYHAAAAAASPSSSSPPTSAGQLPFPIGADEYEFSCSNSPA---- 75

Query: 70  HFPGFHVGKRRRNQHHNSFFACAQAPETLDDDATAVNAVKAVMEMLNNHGGASSS-SVQA 129
            FP FHVGKRRRNQ+HNSFFACA AP+TLDDDA AVNAV AV+E+LNNH GASS+  + A
Sbjct: 76  -FPTFHVGKRRRNQNHNSFFACAHAPDTLDDDAAAVNAVNAVVEILNNHTGASSTPPIPA 135

Query: 130 SPTLPGFGRTP-RVRQLRITDSPFPLQDANADPQVDKAADEFISRFYKELRLQKTADDD 182
           SP LPGFG TP RVRQLRITDSPFPLQDANADP VDKAADEFISRFYKELRLQKTAD++
Sbjct: 136 SPALPGFGPTPRRVRQLRITDSPFPLQDANADPLVDKAADEFISRFYKELRLQKTADEN 184

BLAST of Sgr015674 vs. ExPASy TrEMBL
Match: A0A6J1KDI8 (uncharacterized protein LOC111494650 OS=Cucurbita maxima OX=3661 GN=LOC111494650 PE=4 SV=1)

HSP 1 Score: 231.1 bits (588), Expect = 3.7e-57
Identity = 137/218 (62.84%), Postives = 148/218 (67.89%), Query Frame = 0

Query: 1   MENNLPVIAKKVLNLQSHVPPPLRR------------------------------RHRLL 60
           MENNLP++AKKV NL       LR+                               H   
Sbjct: 1   MENNLPIMAKKVWNLVRVAYFLLRKGISKSKLMLDLNLMTKRGKLAGKAISNLMFHHHYH 60

Query: 61  AAARRSH----EIGHLPFDVGADEYEFSCSNSPAFPGLHFPGFHVGKRRRNQHHNSFFAC 120
           AAA  S       G LPF +GADEYEFSCSNSPA     FP FHVGKRRRNQ+HNSFFAC
Sbjct: 61  AAASPSSSPPTSAGQLPFPIGADEYEFSCSNSPA-----FPAFHVGKRRRNQNHNSFFAC 120

Query: 121 AQAPETLDDD-ATAVNAVKAVMEMLNNHGGASSS-SVQASPTLPGFGRTP-RVRQLRITD 180
           A AP+TLDDD A AVNAV AV+E+LNNH GASS+  V ASP LPGFGRTP RVRQLRITD
Sbjct: 121 AHAPDTLDDDAAAAVNAVNAVVEILNNHSGASSTPPVPASPALPGFGRTPRRVRQLRITD 180

Query: 181 SPFPLQDANADPQVDKAADEFISRFYKELRLQKTADDD 182
           SPFPLQDANADP VDKAADEFISRFYKELRLQKTAD++
Sbjct: 181 SPFPLQDANADPLVDKAADEFISRFYKELRLQKTADEN 213

BLAST of Sgr015674 vs. ExPASy TrEMBL
Match: A0A6J1HJS6 (uncharacterized protein LOC111464220 OS=Cucurbita moschata OX=3662 GN=LOC111464220 PE=4 SV=1)

HSP 1 Score: 226.5 bits (576), Expect = 9.0e-56
Identity = 126/178 (70.79%), Postives = 135/178 (75.84%), Query Frame = 0

Query: 10  KKVLNLQSHVPPPLRRRHRLLAAARRSH----EIGHLPFDVGADEYEFSCSNSPAFPGLH 69
           K + NL  H        H   AAA  S       G LPF VGADEYEFSCSNSPA     
Sbjct: 41  KAISNLMFH-------HHYHAAAASPSSSPPTSAGQLPFPVGADEYEFSCSNSPA----- 100

Query: 70  FPGFHVGKRRRNQHHNSFFACAQAPETLDDDATAVNAVKAVMEMLNNHGGASSS-SVQAS 129
           FP FHVGKRRRNQ+HNSFFACA AP+TLDDDA AVNAV AV+E+LNNH GASS+  + AS
Sbjct: 101 FPAFHVGKRRRNQNHNSFFACAHAPDTLDDDAAAVNAVNAVVEILNNHTGASSTPPIPAS 160

Query: 130 PTLPGFGRTP-RVRQLRITDSPFPLQDANADPQVDKAADEFISRFYKELRLQKTADDD 182
           P LPGFG TP RVRQLRITDSPFPLQDANADP VDKAADEFISRFYKELRLQKTAD++
Sbjct: 161 PALPGFGPTPRRVRQLRITDSPFPLQDANADPLVDKAADEFISRFYKELRLQKTADEN 206

BLAST of Sgr015674 vs. ExPASy TrEMBL
Match: A0A6J1GPL9 (uncharacterized protein LOC111456334 OS=Cucurbita moschata OX=3662 GN=LOC111456334 PE=4 SV=1)

HSP 1 Score: 185.7 bits (470), Expect = 1.8e-43
Identity = 111/212 (52.36%), Postives = 127/212 (59.91%), Query Frame = 0

Query: 1   MENNLPVIAKKVLNLQSHVPPPLRR------------------------------RHRLL 60
           MENNLPVI+K++  L       LR+                               H   
Sbjct: 1   MENNLPVISKRLWKLVRVAYFLLRKGISKSKLILDLNLMMKRGKIAGKAITNLMFHHHYH 60

Query: 61  AAARRSHEIGHLPFDVGADEYEFSCSNSPAFPGLHFPGFHVGKRRRNQHH-NSFFACAQA 120
             A  S     LP  VG D+YEF+CS+SPAFP LHFP FH  KRRRNQ+H +SFFACA A
Sbjct: 61  GGATPSSSADQLPIAVGPDDYEFNCSDSPAFPSLHFPAFH--KRRRNQNHTSSFFACAHA 120

Query: 121 PETLDDDATAVNAVKAVMEMLNNHGGASSSSVQASPTLPGFGRTPRVRQLRITDSPFPLQ 180
           P TLDDDA AVNAVKA +E+ N H GASS S+ +            VRQLRITDSPFPL 
Sbjct: 121 PPTLDDDAAAVNAVKAAVEIFNYHSGASSPSLVSG----------SVRQLRITDSPFPLH 180

Query: 181 DANADPQVDKAADEFISRFYKELRLQKTADDD 182
           DANAD  VDKAADE+ISRFYKELRLQ TAD++
Sbjct: 181 DANADSSVDKAADEYISRFYKELRLQPTADEN 200

BLAST of Sgr015674 vs. ExPASy TrEMBL
Match: A0A6J1JMV1 (uncharacterized protein LOC111488328 OS=Cucurbita maxima OX=3661 GN=LOC111488328 PE=4 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 7.4e-42
Identity = 108/211 (51.18%), Postives = 124/211 (58.77%), Query Frame = 0

Query: 1   MENNLPVIAKKVLNLQSHVPPPLRR------------------------------RHRLL 60
           MENNLPVI+K++  L       LR+                               H   
Sbjct: 1   MENNLPVISKRLWKLVRVAYFLLRKGISKSKLILDLNLMMKRGKIAGKAIANLMFHHHYH 60

Query: 61  AAARRSHEIGHLPFDVGADEYEFSCSNSPAFPGLHFPGFHVGKRRRNQHHNSFFACAQAP 120
             A  S     LP  VG D+YEF+CS+SPAFP LHFP FH  KRRRNQ+H SFFACA AP
Sbjct: 61  GGATPSSSADQLPVAVGPDDYEFNCSDSPAFPSLHFPAFH--KRRRNQNHTSFFACAHAP 120

Query: 121 ETLDDDATAVNAVKAVMEMLNNHGGASSSSVQASPTLPGFGRTPRVRQLRITDSPFPLQD 180
           +TLDDDA    AVKA +E+ N H GASS S+ +            VRQLRITDSPFPL D
Sbjct: 121 QTLDDDAA---AVKAAVEIFNYHSGASSPSLVSG----------SVRQLRITDSPFPLHD 180

Query: 181 ANADPQVDKAADEFISRFYKELRLQKTADDD 182
           ANAD  VDKAADE+ISRFYKELRLQ TAD++
Sbjct: 181 ANADSSVDKAADEYISRFYKELRLQPTADEN 196

BLAST of Sgr015674 vs. ExPASy TrEMBL
Match: A0A1S4E3M3 (actin-related protein 8-like OS=Cucumis melo OX=3656 GN=LOC103500787 PE=4 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 1.9e-37
Identity = 102/208 (49.04%), Postives = 129/208 (62.02%), Query Frame = 0

Query: 1   MENNLPVIAKKVLNL-------------QSHVP----PPLRRRHRLLA------------ 60
           ME+N+PVIAKKV NL             +S V       + +R ++              
Sbjct: 1   MESNVPVIAKKVWNLVRVAYFLIRKGISKSKVNMLDLNLMMKRGKIAGKAISNLMFQHHY 60

Query: 61  AARRSHEIGH-LPFDVGADEYEFSCSNSPAFPGLHFPGFHVGKRRR-NQHHNSFFACAQA 120
           A      + H LPFDV  D+YEFSCSN+P++        + GKRRR NQ++NSFFACA A
Sbjct: 61  AQHHQQNVAHQLPFDVAPDDYEFSCSNTPSY-------HYFGKRRRNNQNNNSFFACAHA 120

Query: 121 PETLDDDATAVNAVKAVMEMLNNHGGASSSSVQASPTLPGFGRTPRVRQLRITDSPFPLQ 178
           P+TLDDD   +NA+KAV+++LNN+   ++++   SP +P       VRQLRITDSPFPLQ
Sbjct: 121 PQTLDDDLPTLNALKAVVDILNNNNNNNNNNPPFSPPIP-------VRQLRITDSPFPLQ 180

BLAST of Sgr015674 vs. TAIR 10
Match: AT1G52140.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G16330.1); Has 114 Blast hits to 114 proteins in 15 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 114; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 114.4 bits (285), Expect = 9.6e-26
Identity = 67/135 (49.63%), Postives = 84/135 (62.22%), Query Frame = 0

Query: 50  EYEFSCSNSPAFPGLHFPGFHVGKRRRNQHHNSFFACAQAPETLDDDATAVNAVKAVMEM 109
           EYEFSCSN+P +    FP  ++   R+ + HN+ F C Q P+TLDDD   V A +AV+E+
Sbjct: 75  EYEFSCSNTPNY---SFPFSNMAFMRK-KSHNNLFTCGQTPQTLDDD---VAAARAVLEL 134

Query: 110 LNNHG-----GASSSSVQASPTLPGFGRTPRVRQLRITDSPFPLQDANAD---PQVDKAA 169
           LN  G       +  +V  SP  PGFG+TP VR LR+TDSPFPL   N D     VDKAA
Sbjct: 135 LNGVGEKGNVTPADLTVALSPYFPGFGQTPLVRPLRVTDSPFPLTPENGDVANGHVDKAA 194

Query: 170 DEFISRFYKELRLQK 177
           D+FI +FYK L  QK
Sbjct: 195 DDFIKKFYKNLNQQK 202

BLAST of Sgr015674 vs. TAIR 10
Match: AT3G16330.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G52140.1); Has 109 Blast hits to 109 proteins in 16 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 109; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 114.0 bits (284), Expect = 1.3e-25
Identity = 71/141 (50.35%), Postives = 94/141 (66.67%), Query Frame = 0

Query: 49  DEYEFSCSNSPAFPGLHFPGFHVGKRRRNQHHNSFFACAQAPETLDDDATAVNAVKAVME 108
           +EYEFSCS++P +    FP F++   ++  HHNS F+C QAP TLDDD T+V+  +AV+E
Sbjct: 64  NEYEFSCSDTPNYT---FP-FNMAAFKKKSHHNSLFSCGQAPPTLDDD-TSVS--RAVLE 123

Query: 109 MLNNHG------GASSSSVQA----SPTLPGFGR-TPRVRQLRITDSPFPLQDAN--ADP 168
           +LN+ G         + S++A    SP LPGFGR T  VR LR+TDSPFPL++    A+ 
Sbjct: 124 LLNSGGDHDQGSNTPAFSIEALTALSPYLPGFGRSTSSVRPLRVTDSPFPLREEGDVANG 183

Query: 169 QVDKAADEFISRFYKELRLQK 177
            VDKAADEFI +FYK L  QK
Sbjct: 184 HVDKAADEFIKKFYKNLYQQK 197

BLAST of Sgr015674 vs. TAIR 10
Match: AT4G29110.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: response to chitin; LOCATED IN: vacuole; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G52140.1); Has 109 Blast hits to 109 proteins in 16 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 109; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 68.2 bits (165), Expect = 7.9e-12
Identity = 48/114 (42.11%), Postives = 64/114 (56.14%), Query Frame = 0

Query: 67  PGFHVGKRRRNQHHNSFFACAQAPETLDDDATAVN-AVKAVMEML--NNHGGASSSSVQA 126
           P   + KR+R  H              D++  AV  AVK V E+L  N+    ++ S + 
Sbjct: 86  PFAFISKRKRRVHGG-----------YDNEEDAVEAAVKKVFELLGENDKKTVATESARE 145

Query: 127 SPTLPGFGRTPRVRQLRITDSPFPLQD-ANADPQVDKAADEFISRFYKELRLQK 177
           SP +     +P VRQLR+TDSPFPL D  + D  VDKAA+EFI +FYK L+LQK
Sbjct: 146 SPLI----MSPAVRQLRVTDSPFPLDDGGDHDHVVDKAAEEFIKKFYKNLKLQK 184