Sgr015311 (gene) Monk fruit (Qingpiguo) v1

Overview


Name	Sgr015311
Type	gene
Organism	Siraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description	glycine-rich protein DC7.1-like
Location	tig00003412: 701657 .. 704039 (-)
RNA-Seq Expression	Sgr015311
Synteny	Sgr015311

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGTTCCAAAGCTTTCATCTTCCTGGGTCTTCTTTTCGCCGTCGTTGTCCTCCTCTCCTCGGAGGAGGTTACGGTTACAGCGAGAGACTTGAAAAGCGGTAAGTTTCATTTCCACAAACCTTCATTTCATGATACATTACAAATGTGCTTAAATTACTTTTACTATAGAGCTTAATTAGTGTTTGCGTTGATTTTGATTGTAAACGCAGTTGAAGAAGCCATGATGGAGACCAACGGAGTCGAGGACGACAAGCGTCGTTTCGGCAGAGGCCACCATGGGCACAGGCATGGCCATTGCCACCACGGTTGCTGCGGTCACCACCACGGCGGCTGTAGCAGGTGCTGCAGCTTTGCTGGTGAGGCTGTTGATGCAGAACCCCAAGCCAAGCCTAACAACTGAACACTGGTGCTTCACAAATAAAGGCTTTCTGAAGCTGACATGTTTGTAATTAAATGTGTTAAGTTATGAATAAATGCATAGGGATGGGATGCCATGTCCTCTTGCCCCCATGTAAACCTGCCCAAGTACAAGGGATTAGTTAAATCTCTTATGTTGAGATTAATTGTTTGTATTTGAAGCTTTATTATATAAGTTAATCACAGTGTCCTGTGAAGTGTTGTTATTAATGACTTTTGTATCAATCCCTACAATCTATGACAACTGCATGACACACACAGATCTCTCGTATAGTGTCAGAAGTGTTTGGTTCTTAAGTTACATGGGTTTAATTATTCTATGTCAAGGGTTTTGCTAATCTCATGGTAATATAAAATTTGGTCACATAAACTCGAGCATTCTAACTCTCGAACTATCTACTTTTTTTTATCAATCACAAACCTATAAACTAAATACAAATTTCAAGGCACAATCCAAATACAAACTATACAAATTTTGTTCTCTAATAATTAAGAGACTACAAGTTTATGGTAGGTTGAGAGAGATCTTTTTCGAGGTTTTAGAGAGAGAGATCTTATTCACACGGCTGGTTGAACATTGATCATGATACATTGGAAGAGAGAGAGTGGCATTTTTCTCTCTTGGTACAAGGTTCAGGATAAGATGAGAGGTTGAAGTGGCTTCGTCCCCTTTTCTTGGTTAAATTATGAATTTGGTCCTTTTTAGTTTGAACTTAATTTCAATATGGTCACTATGATTTTAAAAATGTCAATTTAGCCTCTATAATTTGGCCTCTATAGTTTTTAAAGTGTCATTTGGTCCATACAGTTTAGTTAAACTTCACATACCAATCCTAAACCGTCTTTTACCCACTTGAAAATCCTACCCTGTATTTTCTATTTTTTTATTCATCCTTAAACTTTAATACTTTAGCTAAATAGAATACATCAACCAATTGTCATGTAAATAAAAAAAATGTCACATATTCAATATGGTAACAATAAGAAAAATTTGTGAAATTTGACAAAATGATAAAGATCGAATTAAAAGTTTTAAAATTATAAAGGTCAAATTGAAACTAAGCCTAGACCACAGAGATCAAATTGGAATTTTTAAAACTATAGGGGCCAAATTTACACATATGTCTCAATCATTGAGGGCCAAATTTGTAATTTAACTCGTTTTCTTTCTTCCTTTTTTTTCTTTTGAAATTTTTTTATTGATACATTATTGACTTGTTAAGTCAACTGACAGAAAGACCATTTCAGAGAAAAATTGAAAGCCTACTAAAGGAGTGCCAAAATTATTTTTATATAATAAAGACATCACCTAAATACAAGATAAGAAATTATTCAATCTTATCTATCTTAAAGGTAAGATTGAAGAGAGATGATTCTTCATTTTTCTTTCTCATTTCATAATCATTCTCAATCAGTCCAACACCCAACTAACTTATGGACAGTCTTTAATTGCCACGTTTGAATTGTAGCCATCTCTCTTGCTCTTAATTCTGCCTATAAATTAAAAAGATTCAGAAGGGGTGCAAAATCTTCTCATTCCATCTCCATCAAACCAGCTTCCTACAGAAAACAAAAAGAGTTCTAAGGCTTTAGCTTTCCTCGGTCTTCTCTTCGCCAGTGTTGTCGTTTTCATCTCCTCAGAGGTGCCGGTGGGGGCAAGAGACCTTACACAGACTTCCATCGAGAACAAGAATGGTGAGTTGTTCCTCCTTGTATCTCATGAGCCCGACGCCGCCGGTGGTGCTTATTGGCTTGTTTAATCTGTACTTATTTAAGTTGCAGAGGCCACGGCGGAGACCATTGGCATGGTGGATGCCAAGCACGGCCAGGGAGGACACTGCGGTGGCTACCCTGGCCGCGATGGCTCCAGTGACTACCCTGGCTGCGGTAACTACAATAGATCATGCGTTGGATACCGCGGTTGCTACACTCGTCGATATCCACCGATATGTCTAGAAAAATAG

mRNA sequence

ATGAGTTCCAAAGCTTTCATCTTCCTGGGTCTTCTTTTCGCCGTCGTTGTCCTCCTCTCCTCGGAGGAGGTTACGGTTACAGCGAGAGACTTGAAAAGCGTTGAAGAAGCCATGATGGAGACCAACGGAGTCGAGGACGACAAGCGTCGTTTCGGCAGAGGCCACCATGGGCACAGGCATGGCCATTGCCACCACGGTTGCTGCGGTCACCACCACGGCGGCTGTAGCAGGTGCTGCAGCTTTGCTGAAGGGGTGCAAAATCTTCTCATTCCATCTCCATCAAACCAGCTTCCTACAGAAAACAAAAAGAGTTCTAAGGCTTTAGCTTTCCTCGGTCTTCTCTTCGCCAGTGTTGTCGTTTTCATCTCCTCAGAGGTGCCGGTGGGGGCAAGAGACCTTACACAGACTTCCATCGAGAACAAGAATGTTGCAGAGGCCACGGCGGAGACCATTGGCATGGTGGATGCCAAGCACGGCCAGGGAGGACACTGCGGTGGCTACCCTGGCCGCGATGGCTCCAGTGACTACCCTGGCTGCGGTAACTACAATAGATCATGCGTTGGATACCGCGGTTGCTACACTCGTCGATATCCACCGATATGTCTAGAAAAATAG

Coding sequence (CDS)

ATGAGTTCCAAAGCTTTCATCTTCCTGGGTCTTCTTTTCGCCGTCGTTGTCCTCCTCTCCTCGGAGGAGGTTACGGTTACAGCGAGAGACTTGAAAAGCGTTGAAGAAGCCATGATGGAGACCAACGGAGTCGAGGACGACAAGCGTCGTTTCGGCAGAGGCCACCATGGGCACAGGCATGGCCATTGCCACCACGGTTGCTGCGGTCACCACCACGGCGGCTGTAGCAGGTGCTGCAGCTTTGCTGAAGGGGTGCAAAATCTTCTCATTCCATCTCCATCAAACCAGCTTCCTACAGAAAACAAAAAGAGTTCTAAGGCTTTAGCTTTCCTCGGTCTTCTCTTCGCCAGTGTTGTCGTTTTCATCTCCTCAGAGGTGCCGGTGGGGGCAAGAGACCTTACACAGACTTCCATCGAGAACAAGAATGTTGCAGAGGCCACGGCGGAGACCATTGGCATGGTGGATGCCAAGCACGGCCAGGGAGGACACTGCGGTGGCTACCCTGGCCGCGATGGCTCCAGTGACTACCCTGGCTGCGGTAACTACAATAGATCATGCGTTGGATACCGCGGTTGCTACACTCGTCGATATCCACCGATATGTCTAGAAAAATAG

Protein sequence

MSSKAFIFLGLLFAVVVLLSSEEVTVTARDLKSVEEAMMETNGVEDDKRRFGRGHHGHRHGHCHHGCCGHHHGGCSRCCSFAEGVQNLLIPSPSNQLPTENKKSSKALAFLGLLFASVVVFISSEVPVGARDLTQTSIENKNVAEATAETIGMVDAKHGQGGHCGGYPGRDGSSDYPGCGNYNRSCVGYRGCYTRRYPPICLEK

Homology

[Close]

BLAST of Sgr015311 vs. NCBI nr
Match: XP_038888674.1 (glycine-rich protein-like [Benincasa hispida])

HSP 1 Score: 126.3 bits (316), Expect = 3.0e-25
Identity = 71/85 (83.53%), Postives = 72/85 (84.71%), Query Frame = 0


Query: 1  MSSKAFIFLGLLFAVVVLLSSEEVTVTARDLKSVEEAMMETN--GVEDDKRRFGRGHHGH 60
          MSSKAFIFLG LF VVVLLSSEEVT  A DLKSVE+AMMETN  G ED KRRF RGHHGH
Sbjct: 1  MSSKAFIFLGFLFVVVVLLSSEEVTAIADDLKSVEKAMMETNGGGFEDVKRRFRRGHHGH 60

Query: 61 RHGHCHHGCCGHHH-GGCSRCCSFA 83
          R  HCHHGCCGHHH GGCSRCCSFA
Sbjct: 61 R--HCHHGCCGHHHLGGCSRCCSFA 83

[Close]

BLAST of Sgr015311 vs. NCBI nr
Match: XP_022989495.1 (glycine-rich protein DC7.1-like [Cucurbita maxima])

HSP 1 Score: 100.1 bits (248), Expect = 2.3e-17
Identity = 61/97 (62.89%), Postives = 67/97 (69.07%), Query Frame = 0


Query: 1  MSSKAFIFLGLLFAVVVLLSSEEVTVTARDLKSVEEAMMETN--GVEDDKRRFGRGHHGH 60
          M+SKAFI LGLLFAVV+LLSSEE T TAR LKSVEEA+METN  G+EDDKRRFG GHHGH
Sbjct: 1  MNSKAFIILGLLFAVVILLSSEEATATARGLKSVEEAVMETNGDGIEDDKRRFGGGHHGH 60

Query: 61 RHGHCHHGCCGHHHGGCSRCCSFAEGVQNLLIPSPSN 96
          +H H       HHH  C   CS  E V       P+N
Sbjct: 61 KHHH------RHHHRHCRHGCSADEDVNEDPQAKPNN 91

[Close]

BLAST of Sgr015311 vs. NCBI nr
Match: KAG6589124.1 (hypothetical protein SDJN03_17689, partial [Cucurbita argyrosperma subsp. sororia] >KAG7022826.1 hypothetical protein SDJN02_16562 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 100.1 bits (248), Expect = 2.3e-17
Identity = 62/97 (63.92%), Postives = 68/97 (70.10%), Query Frame = 0


Query: 1  MSSKAFIFLGLLFAVVVLLSSEEVTVTARDLKSVEEAMMETN--GVEDDKRRFGRGHHGH 60
          M+SKAFI LGLLFAVV+LLSSEE + TAR LKSVEEA+METN  G+EDDKRRFG GHHGH
Sbjct: 1  MNSKAFIILGLLFAVVILLSSEEASATARGLKSVEEAVMETNGDGIEDDKRRFGGGHHGH 60

Query: 61 RHGHCHHGCCGHHHGGCSRCCSFAEGVQNLLIPSPSN 96
          RH H HH    HH   C   CS  E V       P+N
Sbjct: 61 RHHHRHHHRHRHH---CRHGCSADEDVNEDPQAKPNN 94

[Close]

BLAST of Sgr015311 vs. NCBI nr
Match: XP_023529828.1 (glycine-rich protein DC7.1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 98.6 bits (244), Expect = 6.6e-17
Identity = 61/97 (62.89%), Postives = 67/97 (69.07%), Query Frame = 0


Query: 1  MSSKAFIFLGLLFAVVVLLSSEEVTVTARDLKSVEEAMMETN--GVEDDKRRFGRGHHGH 60
          M+SKAFI LGLLFAVV+LLSSEE + TAR LKSVEE +METN  G+EDDKRRFG GHHGH
Sbjct: 1  MNSKAFIILGLLFAVVILLSSEEASATARGLKSVEEVVMETNGDGIEDDKRRFGGGHHGH 60

Query: 61 RHGHCHHGCCGHHHGGCSRCCSFAEGVQNLLIPSPSN 96
          RH H HH    HH   C   CS  E V       P+N
Sbjct: 61 RHHHRHHHRHRHH---CRHGCSADEDVNEDPQAKPNN 94

[Close]

BLAST of Sgr015311 vs. NCBI nr
Match: XP_022930749.1 (glycine-rich protein DC7.1-like [Cucurbita moschata])

HSP 1 Score: 98.2 bits (243), Expect = 8.6e-17
Identity = 60/97 (61.86%), Postives = 67/97 (69.07%), Query Frame = 0


Query: 1  MSSKAFIFLGLLFAVVVLLSSEEVTVTARDLKSVEEAMMETN--GVEDDKRRFGRGHHGH 60
          M+SKAFI LGLLF +V+LLSSEE + TAR LKSVEEA+METN  G+EDDKRRFG GHHGH
Sbjct: 1  MNSKAFIILGLLFTIVILLSSEEASATARGLKSVEEAVMETNGDGIEDDKRRFGGGHHGH 60

Query: 61 RHGHCHHGCCGHHHGGCSRCCSFAEGVQNLLIPSPSN 96
          RH H HH    HH   C   CS  E V       P+N
Sbjct: 61 RHHHRHHHRHRHH---CRHGCSADEDVNEDPQAKPNN 94

[Close]

BLAST of Sgr015311 vs. ExPASy Swiss-Prot
Match: P23137 (Glycine-rich protein OS=Nicotiana tabacum OX=4097 GN=GRP PE=2 SV=1)

HSP 1 Score: 45.8 bits (107), Expect = 6.7e-04
Identity = 41/112 (36.61%), Postives = 52/112 (46.43%), Query Frame = 0


Query: 1   MSSKAFIFLGLLFAVVVLLSSEEVTVTARDLKSVEEAMM--ETNGVEDDKR--------- 60
           M SKAF+FLGL  A   L+SSE   V A +L      M     NGV+ D R         
Sbjct: 1   MGSKAFLFLGLCLAFFFLISSE---VVAGELAETSNPMKLDGENGVDVDGRGGYNDVGGD 60

Query: 61  -----RFGRGHHGHRHGHCHHGCCGHHHGGCSRCCSFA-EGVQNLLIPSPSN 96
                  GRG  G++   C +GCC   + GC RCCS+A E +  +    P N
Sbjct: 61  GYYGGGRGRGGGGYKRRGCRYGCCRKGYNGCKRCCSYAGEAMDKVTEAQPHN 109

[Close]

BLAST of Sgr015311 vs. ExPASy TrEMBL
Match: A0A6J1JMI7 (glycine-rich protein DC7.1-like OS=Cucurbita maxima OX=3661 GN=LOC111486537 PE=4 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 1.1e-17
Identity = 61/97 (62.89%), Postives = 67/97 (69.07%), Query Frame = 0


Query: 1  MSSKAFIFLGLLFAVVVLLSSEEVTVTARDLKSVEEAMMETN--GVEDDKRRFGRGHHGH 60
          M+SKAFI LGLLFAVV+LLSSEE T TAR LKSVEEA+METN  G+EDDKRRFG GHHGH
Sbjct: 1  MNSKAFIILGLLFAVVILLSSEEATATARGLKSVEEAVMETNGDGIEDDKRRFGGGHHGH 60

Query: 61 RHGHCHHGCCGHHHGGCSRCCSFAEGVQNLLIPSPSN 96
          +H H       HHH  C   CS  E V       P+N
Sbjct: 61 KHHH------RHHHRHCRHGCSADEDVNEDPQAKPNN 91

[Close]

BLAST of Sgr015311 vs. ExPASy TrEMBL
Match: A0A6J1ESD5 (glycine-rich protein DC7.1-like OS=Cucurbita moschata OX=3662 GN=LOC111437131 PE=4 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 4.2e-17
Identity = 60/97 (61.86%), Postives = 67/97 (69.07%), Query Frame = 0


Query: 1  MSSKAFIFLGLLFAVVVLLSSEEVTVTARDLKSVEEAMMETN--GVEDDKRRFGRGHHGH 60
          M+SKAFI LGLLF +V+LLSSEE + TAR LKSVEEA+METN  G+EDDKRRFG GHHGH
Sbjct: 1  MNSKAFIILGLLFTIVILLSSEEASATARGLKSVEEAVMETNGDGIEDDKRRFGGGHHGH 60

Query: 61 RHGHCHHGCCGHHHGGCSRCCSFAEGVQNLLIPSPSN 96
          RH H HH    HH   C   CS  E V       P+N
Sbjct: 61 RHHHRHHHRHRHH---CRHGCSADEDVNEDPQAKPNN 94

[Close]

BLAST of Sgr015311 vs. ExPASy TrEMBL
Match: A0A6J1C3E0 (glycine-rich protein DC7.1-like OS=Momordica charantia OX=3673 GN=LOC111007997 PE=4 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 8.7e-15
Identity = 58/84 (69.05%), Postives = 60/84 (71.43%), Query Frame = 0


Query: 1  MSSKAFIFLGLLFAVVVLLSSEEVTVTARDLKSVEEAMMET-NGVEDDKRRFGRGHHGHR 60
          MSSK  IFLGLLFAVVVLLSSEEVT TARDLKSVEE M ET NG+EDDK    RGHHGH 
Sbjct: 1  MSSKTLIFLGLLFAVVVLLSSEEVTATARDLKSVEEVMTETNNGIEDDK----RGHHGHH 60

Query: 61 HGHCHHGCCGHHHGGCSRCCSFAE 84
          H H     C HHH      CSFA+
Sbjct: 61 HHHRRRRHCHHHH------CSFAD 74

[Close]

BLAST of Sgr015311 vs. ExPASy TrEMBL
Match: A0A6J1C3T3 (glycine-rich protein 3 short isoform-like OS=Momordica charantia OX=3673 GN=LOC111007996 PE=4 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 7.6e-11
Identity = 58/116 (50.00%), Postives = 66/116 (56.90%), Query Frame = 0


Query: 1   MSSKAFIFLGLLFAVVVLLSSEEVTVTARDLKSVEEAMMETNGVEDDK-RRFGRGH---- 60
           M+SKAFI LGLLFAVVVLLSS+   VTARDLK   E +MET+GVED K R + RGH    
Sbjct: 1   MNSKAFILLGLLFAVVVLLSSD---VTARDLKG--ETVMETDGVEDAKDRSYDRGHGHYG 60

Query: 61  ----------------HGHRHG-------------HCHHGCCGHHHGGCSRCCSFA 83
                           HG  +G             HCHHGCCG +HG C RCCS+A
Sbjct: 61  GGYDRGHSRYGGGYPGHGGHYGGGYPGRGRYGGGRHCHHGCCG-YHGSCGRCCSYA 110

[Close]

BLAST of Sgr015311 vs. ExPASy TrEMBL
Match: A0A6J1GKL6 (glycine-rich protein-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111455223 PE=4 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 1.0e-10
Identity = 60/121 (49.59%), Postives = 69/121 (57.02%), Query Frame = 0


Query: 85  VQNLLIPSPSNQLPTENKKSSKALAFLGLLFASVVVFISSEVPVGARDLTQTSIENKNVA 144
           +Q LLIPS    L    K SSKA  FLGLLFA +V+ ISSE  V ARDL +TS + +N  
Sbjct: 1   MQILLIPSSKFSLFLNTKMSSKAFIFLGLLFA-LVLLISSE--VAARDLAETSTKKEN-- 60

Query: 145 EATAETIGMVDAKHGQGGHCGGYPGR---------DGSSDYPGCGNYNRSC----VGYRG 193
           EATAET G+ DAK+  GG+ GGY GR          G   Y G G Y R C     GYR 
Sbjct: 61  EATAETNGVEDAKY--GGYDGGYGGRGGYGGRGGYGGRGGYGGGGGYGRGCRYGRCGYRC 114

[Close]

BLAST of Sgr015311 vs. TAIR 10
Match: AT2G05530.1 (Glycine-rich protein family )

HSP 1 Score: 41.6 bits (96), Expect = 8.9e-04
Identity = 21/37 (56.76%), Postives = 26/37 (70.27%), Query Frame = 0


Query: 51  FGRGHHGHRHGHCHHGCCGHHHGGCSRCCSFA-EGVQ 87
           +  G HG RHG+C +GCC   + GCSRCCS+A E VQ
Sbjct: 74  YNGGGHGGRHGYCRYGCCYRGYHGCSRCCSYAGEAVQ 110

The following BLAST results are available for this feature:

BLAST of Sgr015311 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038888674.1	3.0e-25	83.53	glycine-rich protein-like [Benincasa hispida]	[more]
XP_022989495.1	2.3e-17	62.89	glycine-rich protein DC7.1-like [Cucurbita maxima]	[more]
KAG6589124.1	2.3e-17	63.92	hypothetical protein SDJN03_17689, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023529828.1	6.6e-17	62.89	glycine-rich protein DC7.1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022930749.1	8.6e-17	61.86	glycine-rich protein DC7.1-like [Cucurbita moschata]	[more]

BLAST of Sgr015311 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P23137	6.7e-04	36.61	Glycine-rich protein OS=Nicotiana tabacum OX=4097 GN=GRP PE=2 SV=1	[more]

BLAST of Sgr015311 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JMI7	1.1e-17	62.89	glycine-rich protein DC7.1-like OS=Cucurbita maxima OX=3661 GN=LOC111486537 PE=4...	[more]
A0A6J1ESD5	4.2e-17	61.86	glycine-rich protein DC7.1-like OS=Cucurbita moschata OX=3662 GN=LOC111437131 PE...	[more]
A0A6J1C3E0	8.7e-15	69.05	glycine-rich protein DC7.1-like OS=Momordica charantia OX=3673 GN=LOC111007997 P...	[more]
A0A6J1C3T3	7.6e-11	50.00	glycine-rich protein 3 short isoform-like OS=Momordica charantia OX=3673 GN=LOC1...	[more]
A0A6J1GKL6	1.0e-10	49.59	glycine-rich protein-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC1114552...	[more]

BLAST of Sgr015311 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G05530.1	8.9e-04	56.76	Glycine-rich protein family	[more]

InterPro

Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR010800	Glycine rich protein	PFAM	PF07172	GRP	coord: 1..74 e-value: 1.9E-9 score: 38.1
IPR010800	Glycine rich protein	PANTHER	PTHR37389	FAMILY NOT NAMED	coord: 1..48
IPR010800	Glycine rich protein	PANTHER	PTHR37389	FAMILY NOT NAMED	coord: 104..191
None	No IPR available	PANTHER	PTHR37389:SF9	GLYCINE-RICH PROTEIN	coord: 1..48
None	No IPR available	PANTHER	PTHR37389:SF9	GLYCINE-RICH PROTEIN	coord: 104..191

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sgr015311.1	Sgr015311.1	mRNA