Sgr014685 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr014685
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
DescriptionIRK-interacting protein
Locationtig00000892: 856118 .. 858263 (-)
RNA-Seq ExpressionSgr014685
SyntenySgr014685
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTTCAGAAGGGCGGCGACCTCAAAAACCGCAACAACGATGTTAGCCGCCAAGAAATTCAGGCGGCCATTGCTAAAGCAGTGGAGCTCAGGGCTCTCCACGCCGCTCTTATGCAAGGAAGCAGCCCCTCTAACCTTCGATTCCCTTCGGCTTCTCCCGTTTCTCATGCTGCTTCTCAATTCTCTGCGCAAGATTATCCTGTTTTCACCCCTGTGAGTTCAAGTTCAACCCCGTTTGCGAAAGATTATCCTTCTCTTTTTTTGAAGTCGAGTACATTAGCTCGCTCTCGTGCTCTTTAATTTTGAACTTTCTTAATTTAAACTCCGTCTATGATTCTTAGATTATGATTTACTGATCTATTGGCATTTATGGTTAGCATTTAATGGCTTTTTCTCTTGAGCTGAGAACATTCTAGGATCACCGTCTTTCTTTTTCACATTCGTATCCGTCATTTTCTCTTAATTGTTTAATTGATGATATTAAGAATTTGGGTATGTTTTGCCTGTGTACGAAACGATTTAGACTAACAAATACATTTATGAGGGGTTAACTGTCAGTCAAAGGTAGAATCAATCTGTTTGCTATAAGTAATTAAAACCTGGGTTTCTGTAAAGTTGGTTGAATTTTGGAAGTCTCTAACAGAGTTATGAAGATGAACCGACGAACGGGTACCACCAGGTTGCGATGAGAATTGGAACTTTTTCAGAAAGCTGGGATGACTATGGATTAGGAGGAGGAAATGAAGATGAGGCAGTTCTATCAGATTATAGGAAGGAGATTTCAAAAGGCTTGGCTTCTGATTTGACCAACTTGGAACCCCATATTTGTCCGGCCGAGGATCACAAGTCTCTCACTGGATCTGCACTTCAAACATCTCCTGCAAACGATTATTTCAAATCGTCTAGGAGGAACAGTTTGGGGGACTTGAAGTCGGCTTCTTCGTGCAATCGATGCAGGCCTGCAACTATTACAAAGGAGAATGACAATACAGCAAAGAATAGCAGGCTTTCAAACACCGTAGTGCCATTGACAGATTCTCACTTTGCAACTCAATCACAACCAAAAAGTCGGGGAGTGATTTCGTGGTTGTTTCCGCGGTTAAAGAAGAAGAATAAGAATGAGAATTCCCCTAACAGGACAGAATCGGAGGATGTCTCTCAGATTTTTAAGGATTTTGGGATAGTGTCGATTGAAACCTTGAAGAAAGAATTGATTGAGGCAAATGAGACAAGAGATGCAGCACTGATGGAGGTTAGCGAGATGAAGTCGTCGTTAGGTGAGCTGAGGCAGAAGCTGGAGGCCTTAGAAACTTATTGTGAGGAACTGAAGAGAGCTTTGAAGCAAGCAACACAAGCAAGAGATCTCCAAACTCCTACCAATTTTCCAAAGAGAATAACATTGAGCGCGGGGACGAATGGAGAGAACCGAATGCCAGTCAGTGAGGAAGTGATGGTGGAAGGCTTCTTGCAGATAGTATCAGAAGCAAGACTATCAGTAAAACAATTCTGCAAGACACTTGTGAGCCAGATTGAAGAAACTGACAGTACCCTGATGGAGAATTTGAATTCACTTCTTCAACCTTACAAACTATCTCTAAACTCCAAGTACTCAAGAGCAGTACTGTACCATTTGGAAGCTATTATCAATCAGGCACTCTACCAAGATTTTGAAAATTGTGTGTTTCAGAAGAATGGCTCCCCCAAGCTGTTAGACCCTCTGCAGGATCGCCAAGCACAGTTCTCGTCGTTTGTGGCGCTGCGAAATCTCAGCTGGAACGAAGTGTTGAGAAAGGGAACGAAATACTACAGCGAAGAGTTCAGCAAGTTCTGTGATCAAAAGATGAGCTGCATAATCACAACTCTGAATTGGACCAGACCATGGCCTGAGCAACTGCTTCAAGCATTCTTCGTGGCGGCCAAATGCATTTGGCTGCTTCACTTGCTTGCCTTCTCGTTCGACCCGCCGCTTAAAATTCTGAGGGTGGAAGACAACAGAAGCTTTGATTCGAGTTACATGGAGGACATGTTTGCGGAGAGGCAGAGAAATGGTCCGAGCCGGGTGAAGATCATGGTGATGCCAGGGTTTTATGTACAAGACAGAATTTTAAGGTGTAAAGTTGTTTGCAGGTACAAGTCACCTTCTTAG

mRNA sequence

ATGTTTCAGAAGGGCGGCGACCTCAAAAACCGCAACAACGATGTTAGCCGCCAAGAAATTCAGGCGGCCATTGCTAAAGCAGTGGAGCTCAGGGCTCTCCACGCCGCTCTTATGCAAGGAAGCAGCCCCTCTAACCTTCGATTCCCTTCGGCTTCTCCCGTTTCTCATGCTGCTTCTCAATTCTCTGCGCAAGATTATCCTGTTTTCACCCCTAGTTATGAAGATGAACCGACGAACGGGTACCACCAGGTTGCGATGAGAATTGGAACTTTTTCAGAAAGCTGGGATGACTATGGATTAGGAGGAGGAAATGAAGATGAGGCAGTTCTATCAGATTATAGGAAGGAGATTTCAAAAGGCTTGGCTTCTGATTTGACCAACTTGGAACCCCATATTTGTCCGGCCGAGGATCACAAGTCTCTCACTGGATCTGCACTTCAAACATCTCCTGCAAACGATTATTTCAAATCGTCTAGGAGGAACAGTTTGGGGGACTTGAAGTCGGCTTCTTCGTGCAATCGATGCAGGCCTGCAACTATTACAAAGGAGAATGACAATACAGCAAAGAATAGCAGGCTTTCAAACACCGTAGTGCCATTGACAGATTCTCACTTTGCAACTCAATCACAACCAAAAAGTCGGGGAGTGATTTCGTGGTTGTTTCCGCGGTTAAAGAAGAAGAATAAGAATGAGAATTCCCCTAACAGGACAGAATCGGAGGATGTCTCTCAGATTTTTAAGGATTTTGGGATAGTGTCGATTGAAACCTTGAAGAAAGAATTGATTGAGGCAAATGAGACAAGAGATGCAGCACTGATGGAGGTTAGCGAGATGAAGTCGTCGTTAGGTGAGCTGAGGCAGAAGCTGGAGGCCTTAGAAACTTATTGTGAGGAACTGAAGAGAGCTTTGAAGCAAGCAACACAAGCAAGAGATCTCCAAACTCCTACCAATTTTCCAAAGAGAATAACATTGAGCGCGGGGACGAATGGAGAGAACCGAATGCCAGTCAGTGAGGAAGTGATGGTGGAAGGCTTCTTGCAGATAGTATCAGAAGCAAGACTATCAGTAAAACAATTCTGCAAGACACTTGTGAGCCAGATTGAAGAAACTGACAGTACCCTGATGGAGAATTTGAATTCACTTCTTCAACCTTACAAACTATCTCTAAACTCCAAGTACTCAAGAGCAGTACTGTACCATTTGGAAGCTATTATCAATCAGGCACTCTACCAAGATTTTGAAAATTGTGTGTTTCAGAAGAATGGCTCCCCCAAGCTGTTAGACCCTCTGCAGGATCGCCAAGCACAGTTCTCGTCGTTTGTGGCGCTGCGAAATCTCAGCTGGAACGAAGTGTTGAGAAAGGGAACGAAATACTACAGCGAAGAGTTCAGCAAGTTCTGTGATCAAAAGATGAGCTGCATAATCACAACTCTGAATTGGACCAGACCATGGCCTGAGCAACTGCTTCAAGCATTCTTCGTGGCGGCCAAATGCATTTGGCTGCTTCACTTGCTTGCCTTCTCGTTCGACCCGCCGCTTAAAATTCTGAGGGTGGAAGACAACAGAAGCTTTGATTCGAGTTACATGGAGGACATGTTTGCGGAGAGGCAGAGAAATGGTCCGAGCCGGGTGAAGATCATGGTGATGCCAGGGTTTTATGTACAAGACAGAATTTTAAGGTGTAAAGTTGTTTGCAGGTACAAGTCACCTTCTTAG

Coding sequence (CDS)

ATGTTTCAGAAGGGCGGCGACCTCAAAAACCGCAACAACGATGTTAGCCGCCAAGAAATTCAGGCGGCCATTGCTAAAGCAGTGGAGCTCAGGGCTCTCCACGCCGCTCTTATGCAAGGAAGCAGCCCCTCTAACCTTCGATTCCCTTCGGCTTCTCCCGTTTCTCATGCTGCTTCTCAATTCTCTGCGCAAGATTATCCTGTTTTCACCCCTAGTTATGAAGATGAACCGACGAACGGGTACCACCAGGTTGCGATGAGAATTGGAACTTTTTCAGAAAGCTGGGATGACTATGGATTAGGAGGAGGAAATGAAGATGAGGCAGTTCTATCAGATTATAGGAAGGAGATTTCAAAAGGCTTGGCTTCTGATTTGACCAACTTGGAACCCCATATTTGTCCGGCCGAGGATCACAAGTCTCTCACTGGATCTGCACTTCAAACATCTCCTGCAAACGATTATTTCAAATCGTCTAGGAGGAACAGTTTGGGGGACTTGAAGTCGGCTTCTTCGTGCAATCGATGCAGGCCTGCAACTATTACAAAGGAGAATGACAATACAGCAAAGAATAGCAGGCTTTCAAACACCGTAGTGCCATTGACAGATTCTCACTTTGCAACTCAATCACAACCAAAAAGTCGGGGAGTGATTTCGTGGTTGTTTCCGCGGTTAAAGAAGAAGAATAAGAATGAGAATTCCCCTAACAGGACAGAATCGGAGGATGTCTCTCAGATTTTTAAGGATTTTGGGATAGTGTCGATTGAAACCTTGAAGAAAGAATTGATTGAGGCAAATGAGACAAGAGATGCAGCACTGATGGAGGTTAGCGAGATGAAGTCGTCGTTAGGTGAGCTGAGGCAGAAGCTGGAGGCCTTAGAAACTTATTGTGAGGAACTGAAGAGAGCTTTGAAGCAAGCAACACAAGCAAGAGATCTCCAAACTCCTACCAATTTTCCAAAGAGAATAACATTGAGCGCGGGGACGAATGGAGAGAACCGAATGCCAGTCAGTGAGGAAGTGATGGTGGAAGGCTTCTTGCAGATAGTATCAGAAGCAAGACTATCAGTAAAACAATTCTGCAAGACACTTGTGAGCCAGATTGAAGAAACTGACAGTACCCTGATGGAGAATTTGAATTCACTTCTTCAACCTTACAAACTATCTCTAAACTCCAAGTACTCAAGAGCAGTACTGTACCATTTGGAAGCTATTATCAATCAGGCACTCTACCAAGATTTTGAAAATTGTGTGTTTCAGAAGAATGGCTCCCCCAAGCTGTTAGACCCTCTGCAGGATCGCCAAGCACAGTTCTCGTCGTTTGTGGCGCTGCGAAATCTCAGCTGGAACGAAGTGTTGAGAAAGGGAACGAAATACTACAGCGAAGAGTTCAGCAAGTTCTGTGATCAAAAGATGAGCTGCATAATCACAACTCTGAATTGGACCAGACCATGGCCTGAGCAACTGCTTCAAGCATTCTTCGTGGCGGCCAAATGCATTTGGCTGCTTCACTTGCTTGCCTTCTCGTTCGACCCGCCGCTTAAAATTCTGAGGGTGGAAGACAACAGAAGCTTTGATTCGAGTTACATGGAGGACATGTTTGCGGAGAGGCAGAGAAATGGTCCGAGCCGGGTGAAGATCATGGTGATGCCAGGGTTTTATGTACAAGACAGAATTTTAAGGTGTAAAGTTGTTTGCAGGTACAAGTCACCTTCTTAG

Protein sequence

MFQKGGDLKNRNNDVSRQEIQAAIAKAVELRALHAALMQGSSPSNLRFPSASPVSHAASQFSAQDYPVFTPSYEDEPTNGYHQVAMRIGTFSESWDDYGLGGGNEDEAVLSDYRKEISKGLASDLTNLEPHICPAEDHKSLTGSALQTSPANDYFKSSRRNSLGDLKSASSCNRCRPATITKENDNTAKNSRLSNTVVPLTDSHFATQSQPKSRGVISWLFPRLKKKNKNENSPNRTESEDVSQIFKDFGIVSIETLKKELIEANETRDAALMEVSEMKSSLGELRQKLEALETYCEELKRALKQATQARDLQTPTNFPKRITLSAGTNGENRMPVSEEVMVEGFLQIVSEARLSVKQFCKTLVSQIEETDSTLMENLNSLLQPYKLSLNSKYSRAVLYHLEAIINQALYQDFENCVFQKNGSPKLLDPLQDRQAQFSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIITTLNWTRPWPEQLLQAFFVAAKCIWLLHLLAFSFDPPLKILRVEDNRSFDSSYMEDMFAERQRNGPSRVKIMVMPGFYVQDRILRCKVVCRYKSPS
Homology
BLAST of Sgr014685 vs. NCBI nr
Match: XP_022148811.1 (IRK-interacting protein [Momordica charantia])

HSP 1 Score: 1028.9 bits (2659), Expect = 1.7e-296
Identity = 530/575 (92.17%), Postives = 542/575 (94.26%), Query Frame = 0

Query: 1   MFQKGGDLKNRNNDVSRQEIQAAIAKAVELRALHAALMQGSSPSNLRFPSASPVSHAASQ 60
           MFQ GG      N+VSRQEIQAAIAKAVELRALHAALMQGSSPSNLRFPSASPVSHAASQ
Sbjct: 6   MFQNGG------NEVSRQEIQAAIAKAVELRALHAALMQGSSPSNLRFPSASPVSHAASQ 65

Query: 61  FSAQDYPVFTPSYEDEPTNGYHQVAMRIGTFSESWDDYGL---GGGNEDEAVLSDYRKEI 120
           FSAQDYPVFTPSYEDEP+NGYHQV MRIGTFSESWD+YGL   GGGNEDE V SDY KE+
Sbjct: 66  FSAQDYPVFTPSYEDEPSNGYHQVPMRIGTFSESWDEYGLGGGGGGNEDETVFSDY-KEV 125

Query: 121 SKGLASDLTNLEPHICPAED-HKSLTGSALQTSPANDYFKSSRRNSLGDLKSASSCNRCR 180
           SKGLASD TN+E HICPAED HK LTGSALQTSPANDYFKSSRRNSLGD KS SSCNRCR
Sbjct: 126 SKGLASDFTNVESHICPAEDLHKPLTGSALQTSPANDYFKSSRRNSLGDSKSTSSCNRCR 185

Query: 181 PATITKENDNTAKNSRLSNTVVPLTDSHFATQSQPKSRGVISWLFPRLKKKNKNENSPNR 240
           PA ITKE+DNTAKNSRLSNT+VPLTDSH ATQSQPKSRGVISWLFPRLKKKNK ENSPNR
Sbjct: 186 PAVITKESDNTAKNSRLSNTIVPLTDSHLATQSQPKSRGVISWLFPRLKKKNKTENSPNR 245

Query: 241 TESEDVSQIFKDFGIVSIETLKKELIEANETRDAALMEVSEMKSSLGELRQKLEALETYC 300
           TESEDVSQIFKD GIVS+ETL+KELIEANE RDAALMEVSEMKSSLGELRQKLE LETYC
Sbjct: 246 TESEDVSQIFKDLGIVSMETLRKELIEANEMRDAALMEVSEMKSSLGELRQKLEGLETYC 305

Query: 301 EELKRALKQATQARDLQTPTNFPKRITLSAGTNGENRMPVSEEVMVEGFLQIVSEARLSV 360
           EELKRALKQAT ARDLQTPTN PKR T + GTNGENRMPVSEEVMVEGFLQIVSEARLSV
Sbjct: 306 EELKRALKQATHARDLQTPTNLPKRTTSNVGTNGENRMPVSEEVMVEGFLQIVSEARLSV 365

Query: 361 KQFCKTLVSQIEETDSTLMENLNSLLQPYKLSLNSKYSRAVLYHLEAIINQALYQDFENC 420
           KQFCKTLV QIEETDSTLMENLNSLLQPYKLSLNSKYSRAVLYHLEA INQALYQDFENC
Sbjct: 366 KQFCKTLVGQIEETDSTLMENLNSLLQPYKLSLNSKYSRAVLYHLEATINQALYQDFENC 425

Query: 421 VFQKNGSPKLLDPLQDRQAQFSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIIT 480
           VFQKNGSPKLLDPLQDRQAQFSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIIT
Sbjct: 426 VFQKNGSPKLLDPLQDRQAQFSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIIT 485

Query: 481 TLNWTRPWPEQLLQAFFVAAKCIWLLHLLAFSFDPPLKILRVEDNRSFDSSYMEDMFAER 540
           TLNWTRPWPEQLLQAFFVAAKCIWLLHLLAFSFDPPLKILRVE+NRSFDSSYMEDMFAER
Sbjct: 486 TLNWTRPWPEQLLQAFFVAAKCIWLLHLLAFSFDPPLKILRVEENRSFDSSYMEDMFAER 545

Query: 541 QRNGPSRVKIMVMPGFYVQDRILRCKVVCRYKSPS 572
           QRNGPSRVKIMVMPGFYVQDRILRCKVVCRYKSP+
Sbjct: 546 QRNGPSRVKIMVMPGFYVQDRILRCKVVCRYKSPT 573

BLAST of Sgr014685 vs. NCBI nr
Match: XP_023534028.1 (IRK-interacting protein [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1028.1 bits (2657), Expect = 2.9e-296
Identity = 523/572 (91.43%), Postives = 543/572 (94.93%), Query Frame = 0

Query: 1   MFQKGGDLKNRNNDVSRQEIQAAIAKAVELRALHAALMQGSSPSNLRFPSASPVSHAASQ 60
           MFQ GG       D+SRQEIQAAIAKAVELRALHAALMQGSSPSN+RFPSASPVS AASQ
Sbjct: 9   MFQNGG-------DISRQEIQAAIAKAVELRALHAALMQGSSPSNVRFPSASPVSQAASQ 68

Query: 61  FSAQDYPVFTPSYEDEPTNGYHQVAMRIGTFSESWDDYGL-GGGNEDEAVLSDYRKEISK 120
           FSAQDYPVFTPSYEDEPTNGYHQV MRIGTFSE+WD+YGL GGGNEDE VLSDYRKEISK
Sbjct: 69  FSAQDYPVFTPSYEDEPTNGYHQVPMRIGTFSETWDEYGLGGGGNEDETVLSDYRKEISK 128

Query: 121 GLASDLTNLEPHICPAEDHKSLTGSALQTSPANDYFKSSRRNSLGDLKSASSCNRCRPAT 180
           GL SD  N EPHICPAE+HK+LTGSA+QTSP NDYFKSSRRNSLGD KS SSCNRCRPAT
Sbjct: 129 GLGSDFANSEPHICPAEEHKALTGSAVQTSPVNDYFKSSRRNSLGDSKSISSCNRCRPAT 188

Query: 181 ITKENDNTAKNSRLSNTVVPLTDSHFATQSQPKSRGVISWLFPRLKKKNKNENSPNRTES 240
           ITKE+DN AKNSRLSNT+VPLTDSH ATQSQPKSRGVISWLFPRLKKKNKNENSPNRTES
Sbjct: 189 ITKESDNAAKNSRLSNTIVPLTDSHLATQSQPKSRGVISWLFPRLKKKNKNENSPNRTES 248

Query: 241 EDVSQIFKDFGIVSIETLKKELIEANETRDAALMEVSEMKSSLGELRQKLEALETYCEEL 300
           EDVSQIFKD GIVSIETLKKELIEANETRDAALMEV E+KSSLGELRQKLE LE+YCEEL
Sbjct: 249 EDVSQIFKDLGIVSIETLKKELIEANETRDAALMEVGEVKSSLGELRQKLEGLESYCEEL 308

Query: 301 KRALKQATQARDLQTPTNFPKRITLSAGTNGENRMPVSEEVMVEGFLQIVSEARLSVKQF 360
           KRALKQAT ARDLQT TN PK+ITL+AGTNGEN+MPVSEEVMVEGFLQIVSEARLSVKQF
Sbjct: 309 KRALKQATNARDLQTVTNLPKKITLNAGTNGENQMPVSEEVMVEGFLQIVSEARLSVKQF 368

Query: 361 CKTLVSQIEETDSTLMENLNSLLQPYKLSLNSKYSRAVLYHLEAIINQALYQDFENCVFQ 420
           CKTLVSQIEETDSTLMENLN+LLQPYKL+LNSKYSRAVLYHLEAIINQALYQDFENCVFQ
Sbjct: 369 CKTLVSQIEETDSTLMENLNALLQPYKLALNSKYSRAVLYHLEAIINQALYQDFENCVFQ 428

Query: 421 KNGSPKLLDPLQDRQAQFSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIITTLN 480
           KNGSPKLLDP QDR+A+FSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIITTLN
Sbjct: 429 KNGSPKLLDPRQDREAKFSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIITTLN 488

Query: 481 WTRPWPEQLLQAFFVAAKCIWLLHLLAFSFDPPLKILRVEDNRSFDSSYMEDMFAERQRN 540
           WTRPWPEQLLQAFFVAAKCIWLLHLLAFSFDPPLKILRVE+NRSFD SYMEDMFAERQRN
Sbjct: 489 WTRPWPEQLLQAFFVAAKCIWLLHLLAFSFDPPLKILRVEENRSFDPSYMEDMFAERQRN 548

Query: 541 GPSRVKIMVMPGFYVQDRILRCKVVCRYKSPS 572
           GPSRVKIMVMPGFYVQDRILRCKVVCRYKSP+
Sbjct: 549 GPSRVKIMVMPGFYVQDRILRCKVVCRYKSPA 573

BLAST of Sgr014685 vs. NCBI nr
Match: XP_022958158.1 (IRK-interacting protein [Cucurbita moschata])

HSP 1 Score: 1027.3 bits (2655), Expect = 5.0e-296
Identity = 523/572 (91.43%), Postives = 543/572 (94.93%), Query Frame = 0

Query: 1   MFQKGGDLKNRNNDVSRQEIQAAIAKAVELRALHAALMQGSSPSNLRFPSASPVSHAASQ 60
           MFQ GG       D+SRQEIQAAIAKAVELRALHAALMQGSSPSN+RFPSASPVS AASQ
Sbjct: 9   MFQNGG-------DISRQEIQAAIAKAVELRALHAALMQGSSPSNVRFPSASPVSQAASQ 68

Query: 61  FSAQDYPVFTPSYEDEPTNGYHQVAMRIGTFSESWDDYGL-GGGNEDEAVLSDYRKEISK 120
           FSAQDYPVFTPSYEDEPTNGYHQV MRIGTFSE+WD+YGL GGGNEDE VLSDYRKEISK
Sbjct: 69  FSAQDYPVFTPSYEDEPTNGYHQVPMRIGTFSETWDEYGLGGGGNEDETVLSDYRKEISK 128

Query: 121 GLASDLTNLEPHICPAEDHKSLTGSALQTSPANDYFKSSRRNSLGDLKSASSCNRCRPAT 180
           GL SD TN EPHICPAE+HK+LTGSA+QTSP NDYFKSSRRNSLGD KS SSCNRCRPAT
Sbjct: 129 GLGSDFTNSEPHICPAEEHKALTGSAVQTSPVNDYFKSSRRNSLGDSKSISSCNRCRPAT 188

Query: 181 ITKENDNTAKNSRLSNTVVPLTDSHFATQSQPKSRGVISWLFPRLKKKNKNENSPNRTES 240
           ITKE+DN AKNSRLSNT+VPLTDSH ATQSQPKSRGVISWLFPRLKKKNKNENSPNRTES
Sbjct: 189 ITKESDNAAKNSRLSNTIVPLTDSHLATQSQPKSRGVISWLFPRLKKKNKNENSPNRTES 248

Query: 241 EDVSQIFKDFGIVSIETLKKELIEANETRDAALMEVSEMKSSLGELRQKLEALETYCEEL 300
           EDVSQIFKD GIVSIETLKKELIEANETRDAALMEV E+KSSLGELRQKLE LE+YCEEL
Sbjct: 249 EDVSQIFKDLGIVSIETLKKELIEANETRDAALMEVGEVKSSLGELRQKLEGLESYCEEL 308

Query: 301 KRALKQATQARDLQTPTNFPKRITLSAGTNGENRMPVSEEVMVEGFLQIVSEARLSVKQF 360
           KRALKQAT ARDLQT T+ PK+I LSAGTNGEN+MPVSEEVMVEGFLQIVSEARLSVKQF
Sbjct: 309 KRALKQATNARDLQTVTDLPKKIALSAGTNGENQMPVSEEVMVEGFLQIVSEARLSVKQF 368

Query: 361 CKTLVSQIEETDSTLMENLNSLLQPYKLSLNSKYSRAVLYHLEAIINQALYQDFENCVFQ 420
           CKTLVSQIEETDSTLMENLN+LLQPYKL+LNSKYSRAVLYHLEAIINQALYQDFENCVFQ
Sbjct: 369 CKTLVSQIEETDSTLMENLNALLQPYKLALNSKYSRAVLYHLEAIINQALYQDFENCVFQ 428

Query: 421 KNGSPKLLDPLQDRQAQFSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIITTLN 480
           KNGSPKLLDP QDR+A+FSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIITTLN
Sbjct: 429 KNGSPKLLDPQQDREAKFSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIITTLN 488

Query: 481 WTRPWPEQLLQAFFVAAKCIWLLHLLAFSFDPPLKILRVEDNRSFDSSYMEDMFAERQRN 540
           WTRPWPEQLLQAFFVAAKCIWLLHLLAFSFDPPLKILRVE+NRSFD SYMEDMFAERQRN
Sbjct: 489 WTRPWPEQLLQAFFVAAKCIWLLHLLAFSFDPPLKILRVEENRSFDPSYMEDMFAERQRN 548

Query: 541 GPSRVKIMVMPGFYVQDRILRCKVVCRYKSPS 572
           GPSRVKIMVMPGFYVQDRILRCKVVCRYKSP+
Sbjct: 549 GPSRVKIMVMPGFYVQDRILRCKVVCRYKSPA 573

BLAST of Sgr014685 vs. NCBI nr
Match: KAG7035839.1 (IRK-interacting protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1026.5 bits (2653), Expect = 8.5e-296
Identity = 523/572 (91.43%), Postives = 542/572 (94.76%), Query Frame = 0

Query: 1   MFQKGGDLKNRNNDVSRQEIQAAIAKAVELRALHAALMQGSSPSNLRFPSASPVSHAASQ 60
           MFQ GG       D+SRQEIQAAIAKAVELRALHAALMQGSSPSN+RFPSASPVS AASQ
Sbjct: 9   MFQNGG-------DISRQEIQAAIAKAVELRALHAALMQGSSPSNVRFPSASPVSQAASQ 68

Query: 61  FSAQDYPVFTPSYEDEPTNGYHQVAMRIGTFSESWDDYGL-GGGNEDEAVLSDYRKEISK 120
           FSAQDYPVFTPSYEDEPTNGYHQV MRIGTFSE+WD+YGL GGGNEDE VLSDYRKEISK
Sbjct: 69  FSAQDYPVFTPSYEDEPTNGYHQVPMRIGTFSETWDEYGLGGGGNEDETVLSDYRKEISK 128

Query: 121 GLASDLTNLEPHICPAEDHKSLTGSALQTSPANDYFKSSRRNSLGDLKSASSCNRCRPAT 180
           GL SD  N EPHICPAE+HK+LTGSA+QTSP NDYFKSSRRNSLGD KS SSCNRCRPAT
Sbjct: 129 GLGSDFANSEPHICPAEEHKALTGSAVQTSPVNDYFKSSRRNSLGDSKSISSCNRCRPAT 188

Query: 181 ITKENDNTAKNSRLSNTVVPLTDSHFATQSQPKSRGVISWLFPRLKKKNKNENSPNRTES 240
           ITKE+DN AKNSRLSNT+VPLTDSH ATQSQPKSRGVISWLFPRLKKKNKNENSPNRTES
Sbjct: 189 ITKESDNAAKNSRLSNTIVPLTDSHLATQSQPKSRGVISWLFPRLKKKNKNENSPNRTES 248

Query: 241 EDVSQIFKDFGIVSIETLKKELIEANETRDAALMEVSEMKSSLGELRQKLEALETYCEEL 300
           EDVSQIFKD GIVSIETLKKELIEANETRDAALMEV E+KSSLGELRQKLE LE+YCEEL
Sbjct: 249 EDVSQIFKDLGIVSIETLKKELIEANETRDAALMEVGEVKSSLGELRQKLEGLESYCEEL 308

Query: 301 KRALKQATQARDLQTPTNFPKRITLSAGTNGENRMPVSEEVMVEGFLQIVSEARLSVKQF 360
           KRALKQAT ARDLQT TN PK+ITLSAGTNGEN+MPVSEEVMVEGFLQIVSEARLSVKQF
Sbjct: 309 KRALKQATNARDLQTVTNLPKKITLSAGTNGENQMPVSEEVMVEGFLQIVSEARLSVKQF 368

Query: 361 CKTLVSQIEETDSTLMENLNSLLQPYKLSLNSKYSRAVLYHLEAIINQALYQDFENCVFQ 420
           CKTLVSQIEETDSTLMENLN+LLQPYKL+LNSKYSRAVLYHLEAIINQALYQDFENCVFQ
Sbjct: 369 CKTLVSQIEETDSTLMENLNALLQPYKLALNSKYSRAVLYHLEAIINQALYQDFENCVFQ 428

Query: 421 KNGSPKLLDPLQDRQAQFSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIITTLN 480
           KNGSPKLLDP QDR+A+FSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIITTLN
Sbjct: 429 KNGSPKLLDPQQDREAKFSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIITTLN 488

Query: 481 WTRPWPEQLLQAFFVAAKCIWLLHLLAFSFDPPLKILRVEDNRSFDSSYMEDMFAERQRN 540
           WTRPWPEQLLQAFFVAAKCIWLLHLLAFSFDPPLKILRVE+NRSFD SYMEDMFAERQRN
Sbjct: 489 WTRPWPEQLLQAFFVAAKCIWLLHLLAFSFDPPLKILRVEENRSFDPSYMEDMFAERQRN 548

Query: 541 GPSRVKIMVMPGFYVQDRILRCKVVCRYKSPS 572
           GPSRVKIMVMPGFYVQ RILRCKVVCRYKSP+
Sbjct: 549 GPSRVKIMVMPGFYVQHRILRCKVVCRYKSPA 573

BLAST of Sgr014685 vs. NCBI nr
Match: XP_022995055.1 (IRK-interacting protein [Cucurbita maxima])

HSP 1 Score: 1023.1 bits (2644), Expect = 9.4e-295
Identity = 521/572 (91.08%), Postives = 542/572 (94.76%), Query Frame = 0

Query: 1   MFQKGGDLKNRNNDVSRQEIQAAIAKAVELRALHAALMQGSSPSNLRFPSASPVSHAASQ 60
           MFQ GG       D+SRQEIQAAIAKAVELRALHAALMQGSSPSN+RFPSASPVS AASQ
Sbjct: 9   MFQNGG-------DISRQEIQAAIAKAVELRALHAALMQGSSPSNVRFPSASPVSQAASQ 68

Query: 61  FSAQDYPVFTPSYEDEPTNGYHQVAMRIGTFSESWDDYGL-GGGNEDEAVLSDYRKEISK 120
           FSAQDYPVFTPSYEDEPT GYHQV MRIGTFSE+W++YGL GGGNEDE VLSDYRKEISK
Sbjct: 69  FSAQDYPVFTPSYEDEPTTGYHQVPMRIGTFSETWEEYGLGGGGNEDETVLSDYRKEISK 128

Query: 121 GLASDLTNLEPHICPAEDHKSLTGSALQTSPANDYFKSSRRNSLGDLKSASSCNRCRPAT 180
           GL SD TN EPHICPAE+HK+LTGSA+QTSP NDYFKSSRRNSLGD KS SSCNRCRPAT
Sbjct: 129 GLGSDFTNSEPHICPAEEHKALTGSAVQTSPVNDYFKSSRRNSLGDSKSISSCNRCRPAT 188

Query: 181 ITKENDNTAKNSRLSNTVVPLTDSHFATQSQPKSRGVISWLFPRLKKKNKNENSPNRTES 240
           ITKE+DN AKNSRLSNT+VPLTDSH ATQSQ KSRGVISWLFPRLKKKNKNENSPNRTES
Sbjct: 189 ITKESDNAAKNSRLSNTIVPLTDSHLATQSQSKSRGVISWLFPRLKKKNKNENSPNRTES 248

Query: 241 EDVSQIFKDFGIVSIETLKKELIEANETRDAALMEVSEMKSSLGELRQKLEALETYCEEL 300
           EDVSQIFKD GIVSIETLKKELIEANETRDAAL+EV E+KSSLGELRQKLE LE+YCEEL
Sbjct: 249 EDVSQIFKDLGIVSIETLKKELIEANETRDAALLEVGEVKSSLGELRQKLEGLESYCEEL 308

Query: 301 KRALKQATQARDLQTPTNFPKRITLSAGTNGENRMPVSEEVMVEGFLQIVSEARLSVKQF 360
           KRALKQAT ARDLQT TN PK+ITLSAGTNGEN+MPVSEEVMVEGFLQIVSEARLSVKQF
Sbjct: 309 KRALKQATNARDLQTVTNLPKKITLSAGTNGENQMPVSEEVMVEGFLQIVSEARLSVKQF 368

Query: 361 CKTLVSQIEETDSTLMENLNSLLQPYKLSLNSKYSRAVLYHLEAIINQALYQDFENCVFQ 420
           CKTLVSQIEETDSTLMENLN+LLQPYKL+LNSKYSRAVLYHLEAIINQALYQDFENCVFQ
Sbjct: 369 CKTLVSQIEETDSTLMENLNALLQPYKLALNSKYSRAVLYHLEAIINQALYQDFENCVFQ 428

Query: 421 KNGSPKLLDPLQDRQAQFSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIITTLN 480
           KNGSPKLLDP QDR+A+FSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIITTLN
Sbjct: 429 KNGSPKLLDPQQDREAKFSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIITTLN 488

Query: 481 WTRPWPEQLLQAFFVAAKCIWLLHLLAFSFDPPLKILRVEDNRSFDSSYMEDMFAERQRN 540
           WTRPWPEQLLQAFFVAAKCIWLLHLLAFSFDPPLKILRVE+NRSFD SYMEDMFAERQRN
Sbjct: 489 WTRPWPEQLLQAFFVAAKCIWLLHLLAFSFDPPLKILRVEENRSFDPSYMEDMFAERQRN 548

Query: 541 GPSRVKIMVMPGFYVQDRILRCKVVCRYKSPS 572
           GPSRVKIMVMPGFYVQDRILRCKVVCRYKSP+
Sbjct: 549 GPSRVKIMVMPGFYVQDRILRCKVVCRYKSPA 573

BLAST of Sgr014685 vs. ExPASy Swiss-Prot
Match: Q9LXU9 (IRK-interacting protein OS=Arabidopsis thaliana OX=3702 GN=IRKI PE=1 SV=1)

HSP 1 Score: 576.6 bits (1485), Expect = 3.0e-163
Identity = 332/571 (58.14%), Postives = 410/571 (71.80%), Query Frame = 0

Query: 18  QEIQAAIAKAVELRALHAALMQ-GSSPS---NLRFPS----ASPVSHAASQFSAQDYPVF 77
           +E + AIA  V+LRAL A+LMQ  SSPS   +LR PS    ASP S      SA DYPVF
Sbjct: 12  REKEEAIANGVKLRALQASLMQMKSSPSSNYSLRNPSSSSAASPASRPLPNLSAHDYPVF 71

Query: 78  TPSYEDEPTNGYHQVAMRIGTFSESWDDYGLGGGNEDEAVLSDYRKEISKGLASDLTNLE 137
           TPSYEDEP + +H   +   T SE+WD+ G+G  + D  +   Y+   S+      T + 
Sbjct: 72  TPSYEDEPVSAFHHKNL---TLSETWDEDGVGLVDGDTYLSDSYKTSTSR-----KTVMM 131

Query: 138 PHICPAEDHKSLTGSALQTSPANDYFKSSRRNSLGDLKSASSCNRCRPATITKENDNTAK 197
           PH   +  H      AL++ P + Y          D +S SSCN        K+     K
Sbjct: 132 PH-QDSHHHVYTMSDALRSPPLHFYTTGRSNCGSVDFRSVSSCN-----DYNKQKGFDTK 191

Query: 198 NSRLSNTVVPLTDSHFA-TQSQPKSRG--VISWLFPRLKKKNKNE---NSPNRTE-SEDV 257
           + + SN VVPLTDSH A   SQP++RG  V+SWLFP+LKKK K+    NSP+ TE SE+V
Sbjct: 192 SLKSSNLVVPLTDSHSAVVSSQPRNRGGRVMSWLFPKLKKKQKSNSIFNSPSITEKSEEV 251

Query: 258 SQIFKDFGIVSIETLKKELIEANETRDAALMEVSEMKSSLGELRQKLEALETYCEELKRA 317
           S++ KD G   +E LK+EL+EAN +RDAAL +VSEMKSSLGEL +KL+ LE+YC+ LK+A
Sbjct: 252 SEVLKDSG-SGVEKLKRELMEANRSRDAALTQVSEMKSSLGELSEKLQYLESYCDNLKKA 311

Query: 318 LKQATQARDLQTPTNFPKRITLSAGTNGENRMPVSEEVMVEGFLQIVSEARLSVKQFCKT 377
           L++AT+    +           S+G    + MPVSEEVMVEGFLQIVSEARLS+KQF KT
Sbjct: 312 LREATEVVSQENSGG------RSSGKK-NSEMPVSEEVMVEGFLQIVSEARLSIKQFLKT 371

Query: 378 LVSQIEETDSTLMENLNSLLQPYKLSLNSKYSRAVLYHLEAIINQALYQDFENCVFQKNG 437
           LVS+I+E DSTL+ N+N+LLQP+ LS  SKYS+ + YHLEAII+Q++YQDFENCVFQKNG
Sbjct: 372 LVSEIDEEDSTLIGNINTLLQPHNLSFTSKYSKIIQYHLEAIISQSVYQDFENCVFQKNG 431

Query: 438 SPKLLDPLQDRQAQFSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIITTLNWTR 497
            PKLLDP QDRQA FSSF +LRNLSWNEVL+KGTKYYS+EFS+FCD+KMS IITTLNWTR
Sbjct: 432 KPKLLDPEQDRQANFSSFASLRNLSWNEVLKKGTKYYSDEFSRFCDEKMSLIITTLNWTR 491

Query: 498 PWPEQLLQAFFVAAKCIWLLHLLAFSFDPPLKILRVEDNRSFDSSYMEDMFAERQRN--- 557
           PW EQ+LQAFFVAAKC+WLLHLLAFSF+P L ILRVE+NR F+SS+MEDM A+RQR+   
Sbjct: 492 PWSEQMLQAFFVAAKCVWLLHLLAFSFNPALGILRVEENREFESSFMEDMGADRQRSALS 551

Query: 558 -GPSRVKIMVMPGFYVQDRILRCKVVCRYKS 570
            GP+RVK+MVMPGFYV DR+LRCKV+CRYKS
Sbjct: 552 RGPARVKVMVMPGFYVLDRVLRCKVLCRYKS 560

BLAST of Sgr014685 vs. ExPASy Swiss-Prot
Match: F4KGE8 (Protein GRAVITROPIC IN THE LIGHT 1 OS=Arabidopsis thaliana OX=3702 GN=GIL1 PE=1 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 5.3e-06
Identity = 73/308 (23.70%), Postives = 127/308 (41.23%), Query Frame = 0

Query: 275 VSEMKSSLGELRQKLEALETYCEELKRALKQATQARDLQTPTNFPKRITLSAGTNGENRM 334
           V+  ++ + EL+++++  +T  E LK  +K A+ A       N  K+  L +        
Sbjct: 239 VAPYEAVVKELKKEVKVKDTEIENLKEKVKVASMANG-----NGGKKHRLLSSRKVNCTT 298

Query: 335 PVSEEVMVEGFLQIVSEARLSVKQFCKTLVSQIEETDSTLMENLNSL-----------LQ 394
            ++   + E F   + + + + K F   L+S +      +   + S+             
Sbjct: 299 QIAVSPVPELFEMTMIQVKEASKSFTGILLSLMRAAHWDIAAAVRSIEAASASSDGMSAS 358

Query: 395 PYKLSLNSKY-SRAVLYHLEAIINQALYQDFENCVFQKNGS-PKLLDPLQDRQAQFSSFV 454
            +  S+ S   ++   + LE+ I + ++Q F++  F  +GS   L++P Q R+  F+ F 
Sbjct: 359 SFASSVQSSVPNQHAKFALESYICRKIFQGFDHETFYMDGSLSSLINPDQYRRDCFAQFK 418

Query: 455 ALRNLSWNEVLR----------KGTKYYS-----EEFSKFCDQKMSCIITTLNWTRPWPE 514
            ++ +   E+L              KY S      E S F D +   ++   N  R    
Sbjct: 419 DMKAMDPMELLGILPTCHFGKFCSKKYLSIIHQKMEESLFGDSEQRELVVAGNHPR---S 478

Query: 515 QLLQAFFVAAKCIWLLHLLAFSFDPPLKILRVEDNRSFDSSYMEDM--FAERQRNGPSRV 553
           Q    F   AK +WLLHLLAFS DP            F S YME +  F++ +      V
Sbjct: 479 QFYGEFLGLAKAVWLLHLLAFSLDPSPSHFEANRGAEFHSQYMESVVRFSDGRVPAGQVV 538

BLAST of Sgr014685 vs. ExPASy TrEMBL
Match: A0A6J1D6H9 (IRK-interacting protein OS=Momordica charantia OX=3673 GN=LOC111017373 PE=4 SV=1)

HSP 1 Score: 1028.9 bits (2659), Expect = 8.3e-297
Identity = 530/575 (92.17%), Postives = 542/575 (94.26%), Query Frame = 0

Query: 1   MFQKGGDLKNRNNDVSRQEIQAAIAKAVELRALHAALMQGSSPSNLRFPSASPVSHAASQ 60
           MFQ GG      N+VSRQEIQAAIAKAVELRALHAALMQGSSPSNLRFPSASPVSHAASQ
Sbjct: 6   MFQNGG------NEVSRQEIQAAIAKAVELRALHAALMQGSSPSNLRFPSASPVSHAASQ 65

Query: 61  FSAQDYPVFTPSYEDEPTNGYHQVAMRIGTFSESWDDYGL---GGGNEDEAVLSDYRKEI 120
           FSAQDYPVFTPSYEDEP+NGYHQV MRIGTFSESWD+YGL   GGGNEDE V SDY KE+
Sbjct: 66  FSAQDYPVFTPSYEDEPSNGYHQVPMRIGTFSESWDEYGLGGGGGGNEDETVFSDY-KEV 125

Query: 121 SKGLASDLTNLEPHICPAED-HKSLTGSALQTSPANDYFKSSRRNSLGDLKSASSCNRCR 180
           SKGLASD TN+E HICPAED HK LTGSALQTSPANDYFKSSRRNSLGD KS SSCNRCR
Sbjct: 126 SKGLASDFTNVESHICPAEDLHKPLTGSALQTSPANDYFKSSRRNSLGDSKSTSSCNRCR 185

Query: 181 PATITKENDNTAKNSRLSNTVVPLTDSHFATQSQPKSRGVISWLFPRLKKKNKNENSPNR 240
           PA ITKE+DNTAKNSRLSNT+VPLTDSH ATQSQPKSRGVISWLFPRLKKKNK ENSPNR
Sbjct: 186 PAVITKESDNTAKNSRLSNTIVPLTDSHLATQSQPKSRGVISWLFPRLKKKNKTENSPNR 245

Query: 241 TESEDVSQIFKDFGIVSIETLKKELIEANETRDAALMEVSEMKSSLGELRQKLEALETYC 300
           TESEDVSQIFKD GIVS+ETL+KELIEANE RDAALMEVSEMKSSLGELRQKLE LETYC
Sbjct: 246 TESEDVSQIFKDLGIVSMETLRKELIEANEMRDAALMEVSEMKSSLGELRQKLEGLETYC 305

Query: 301 EELKRALKQATQARDLQTPTNFPKRITLSAGTNGENRMPVSEEVMVEGFLQIVSEARLSV 360
           EELKRALKQAT ARDLQTPTN PKR T + GTNGENRMPVSEEVMVEGFLQIVSEARLSV
Sbjct: 306 EELKRALKQATHARDLQTPTNLPKRTTSNVGTNGENRMPVSEEVMVEGFLQIVSEARLSV 365

Query: 361 KQFCKTLVSQIEETDSTLMENLNSLLQPYKLSLNSKYSRAVLYHLEAIINQALYQDFENC 420
           KQFCKTLV QIEETDSTLMENLNSLLQPYKLSLNSKYSRAVLYHLEA INQALYQDFENC
Sbjct: 366 KQFCKTLVGQIEETDSTLMENLNSLLQPYKLSLNSKYSRAVLYHLEATINQALYQDFENC 425

Query: 421 VFQKNGSPKLLDPLQDRQAQFSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIIT 480
           VFQKNGSPKLLDPLQDRQAQFSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIIT
Sbjct: 426 VFQKNGSPKLLDPLQDRQAQFSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIIT 485

Query: 481 TLNWTRPWPEQLLQAFFVAAKCIWLLHLLAFSFDPPLKILRVEDNRSFDSSYMEDMFAER 540
           TLNWTRPWPEQLLQAFFVAAKCIWLLHLLAFSFDPPLKILRVE+NRSFDSSYMEDMFAER
Sbjct: 486 TLNWTRPWPEQLLQAFFVAAKCIWLLHLLAFSFDPPLKILRVEENRSFDSSYMEDMFAER 545

Query: 541 QRNGPSRVKIMVMPGFYVQDRILRCKVVCRYKSPS 572
           QRNGPSRVKIMVMPGFYVQDRILRCKVVCRYKSP+
Sbjct: 546 QRNGPSRVKIMVMPGFYVQDRILRCKVVCRYKSPT 573

BLAST of Sgr014685 vs. ExPASy TrEMBL
Match: A0A6J1H2D1 (IRK-interacting protein OS=Cucurbita moschata OX=3662 GN=LOC111459468 PE=4 SV=1)

HSP 1 Score: 1027.3 bits (2655), Expect = 2.4e-296
Identity = 523/572 (91.43%), Postives = 543/572 (94.93%), Query Frame = 0

Query: 1   MFQKGGDLKNRNNDVSRQEIQAAIAKAVELRALHAALMQGSSPSNLRFPSASPVSHAASQ 60
           MFQ GG       D+SRQEIQAAIAKAVELRALHAALMQGSSPSN+RFPSASPVS AASQ
Sbjct: 9   MFQNGG-------DISRQEIQAAIAKAVELRALHAALMQGSSPSNVRFPSASPVSQAASQ 68

Query: 61  FSAQDYPVFTPSYEDEPTNGYHQVAMRIGTFSESWDDYGL-GGGNEDEAVLSDYRKEISK 120
           FSAQDYPVFTPSYEDEPTNGYHQV MRIGTFSE+WD+YGL GGGNEDE VLSDYRKEISK
Sbjct: 69  FSAQDYPVFTPSYEDEPTNGYHQVPMRIGTFSETWDEYGLGGGGNEDETVLSDYRKEISK 128

Query: 121 GLASDLTNLEPHICPAEDHKSLTGSALQTSPANDYFKSSRRNSLGDLKSASSCNRCRPAT 180
           GL SD TN EPHICPAE+HK+LTGSA+QTSP NDYFKSSRRNSLGD KS SSCNRCRPAT
Sbjct: 129 GLGSDFTNSEPHICPAEEHKALTGSAVQTSPVNDYFKSSRRNSLGDSKSISSCNRCRPAT 188

Query: 181 ITKENDNTAKNSRLSNTVVPLTDSHFATQSQPKSRGVISWLFPRLKKKNKNENSPNRTES 240
           ITKE+DN AKNSRLSNT+VPLTDSH ATQSQPKSRGVISWLFPRLKKKNKNENSPNRTES
Sbjct: 189 ITKESDNAAKNSRLSNTIVPLTDSHLATQSQPKSRGVISWLFPRLKKKNKNENSPNRTES 248

Query: 241 EDVSQIFKDFGIVSIETLKKELIEANETRDAALMEVSEMKSSLGELRQKLEALETYCEEL 300
           EDVSQIFKD GIVSIETLKKELIEANETRDAALMEV E+KSSLGELRQKLE LE+YCEEL
Sbjct: 249 EDVSQIFKDLGIVSIETLKKELIEANETRDAALMEVGEVKSSLGELRQKLEGLESYCEEL 308

Query: 301 KRALKQATQARDLQTPTNFPKRITLSAGTNGENRMPVSEEVMVEGFLQIVSEARLSVKQF 360
           KRALKQAT ARDLQT T+ PK+I LSAGTNGEN+MPVSEEVMVEGFLQIVSEARLSVKQF
Sbjct: 309 KRALKQATNARDLQTVTDLPKKIALSAGTNGENQMPVSEEVMVEGFLQIVSEARLSVKQF 368

Query: 361 CKTLVSQIEETDSTLMENLNSLLQPYKLSLNSKYSRAVLYHLEAIINQALYQDFENCVFQ 420
           CKTLVSQIEETDSTLMENLN+LLQPYKL+LNSKYSRAVLYHLEAIINQALYQDFENCVFQ
Sbjct: 369 CKTLVSQIEETDSTLMENLNALLQPYKLALNSKYSRAVLYHLEAIINQALYQDFENCVFQ 428

Query: 421 KNGSPKLLDPLQDRQAQFSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIITTLN 480
           KNGSPKLLDP QDR+A+FSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIITTLN
Sbjct: 429 KNGSPKLLDPQQDREAKFSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIITTLN 488

Query: 481 WTRPWPEQLLQAFFVAAKCIWLLHLLAFSFDPPLKILRVEDNRSFDSSYMEDMFAERQRN 540
           WTRPWPEQLLQAFFVAAKCIWLLHLLAFSFDPPLKILRVE+NRSFD SYMEDMFAERQRN
Sbjct: 489 WTRPWPEQLLQAFFVAAKCIWLLHLLAFSFDPPLKILRVEENRSFDPSYMEDMFAERQRN 548

Query: 541 GPSRVKIMVMPGFYVQDRILRCKVVCRYKSPS 572
           GPSRVKIMVMPGFYVQDRILRCKVVCRYKSP+
Sbjct: 549 GPSRVKIMVMPGFYVQDRILRCKVVCRYKSPA 573

BLAST of Sgr014685 vs. ExPASy TrEMBL
Match: A0A6J1K320 (IRK-interacting protein OS=Cucurbita maxima OX=3661 GN=LOC111490720 PE=4 SV=1)

HSP 1 Score: 1023.1 bits (2644), Expect = 4.5e-295
Identity = 521/572 (91.08%), Postives = 542/572 (94.76%), Query Frame = 0

Query: 1   MFQKGGDLKNRNNDVSRQEIQAAIAKAVELRALHAALMQGSSPSNLRFPSASPVSHAASQ 60
           MFQ GG       D+SRQEIQAAIAKAVELRALHAALMQGSSPSN+RFPSASPVS AASQ
Sbjct: 9   MFQNGG-------DISRQEIQAAIAKAVELRALHAALMQGSSPSNVRFPSASPVSQAASQ 68

Query: 61  FSAQDYPVFTPSYEDEPTNGYHQVAMRIGTFSESWDDYGL-GGGNEDEAVLSDYRKEISK 120
           FSAQDYPVFTPSYEDEPT GYHQV MRIGTFSE+W++YGL GGGNEDE VLSDYRKEISK
Sbjct: 69  FSAQDYPVFTPSYEDEPTTGYHQVPMRIGTFSETWEEYGLGGGGNEDETVLSDYRKEISK 128

Query: 121 GLASDLTNLEPHICPAEDHKSLTGSALQTSPANDYFKSSRRNSLGDLKSASSCNRCRPAT 180
           GL SD TN EPHICPAE+HK+LTGSA+QTSP NDYFKSSRRNSLGD KS SSCNRCRPAT
Sbjct: 129 GLGSDFTNSEPHICPAEEHKALTGSAVQTSPVNDYFKSSRRNSLGDSKSISSCNRCRPAT 188

Query: 181 ITKENDNTAKNSRLSNTVVPLTDSHFATQSQPKSRGVISWLFPRLKKKNKNENSPNRTES 240
           ITKE+DN AKNSRLSNT+VPLTDSH ATQSQ KSRGVISWLFPRLKKKNKNENSPNRTES
Sbjct: 189 ITKESDNAAKNSRLSNTIVPLTDSHLATQSQSKSRGVISWLFPRLKKKNKNENSPNRTES 248

Query: 241 EDVSQIFKDFGIVSIETLKKELIEANETRDAALMEVSEMKSSLGELRQKLEALETYCEEL 300
           EDVSQIFKD GIVSIETLKKELIEANETRDAAL+EV E+KSSLGELRQKLE LE+YCEEL
Sbjct: 249 EDVSQIFKDLGIVSIETLKKELIEANETRDAALLEVGEVKSSLGELRQKLEGLESYCEEL 308

Query: 301 KRALKQATQARDLQTPTNFPKRITLSAGTNGENRMPVSEEVMVEGFLQIVSEARLSVKQF 360
           KRALKQAT ARDLQT TN PK+ITLSAGTNGEN+MPVSEEVMVEGFLQIVSEARLSVKQF
Sbjct: 309 KRALKQATNARDLQTVTNLPKKITLSAGTNGENQMPVSEEVMVEGFLQIVSEARLSVKQF 368

Query: 361 CKTLVSQIEETDSTLMENLNSLLQPYKLSLNSKYSRAVLYHLEAIINQALYQDFENCVFQ 420
           CKTLVSQIEETDSTLMENLN+LLQPYKL+LNSKYSRAVLYHLEAIINQALYQDFENCVFQ
Sbjct: 369 CKTLVSQIEETDSTLMENLNALLQPYKLALNSKYSRAVLYHLEAIINQALYQDFENCVFQ 428

Query: 421 KNGSPKLLDPLQDRQAQFSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIITTLN 480
           KNGSPKLLDP QDR+A+FSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIITTLN
Sbjct: 429 KNGSPKLLDPQQDREAKFSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIITTLN 488

Query: 481 WTRPWPEQLLQAFFVAAKCIWLLHLLAFSFDPPLKILRVEDNRSFDSSYMEDMFAERQRN 540
           WTRPWPEQLLQAFFVAAKCIWLLHLLAFSFDPPLKILRVE+NRSFD SYMEDMFAERQRN
Sbjct: 489 WTRPWPEQLLQAFFVAAKCIWLLHLLAFSFDPPLKILRVEENRSFDPSYMEDMFAERQRN 548

Query: 541 GPSRVKIMVMPGFYVQDRILRCKVVCRYKSPS 572
           GPSRVKIMVMPGFYVQDRILRCKVVCRYKSP+
Sbjct: 549 GPSRVKIMVMPGFYVQDRILRCKVVCRYKSPA 573

BLAST of Sgr014685 vs. ExPASy TrEMBL
Match: A0A0A0KZA9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G290210 PE=4 SV=1)

HSP 1 Score: 986.9 bits (2550), Expect = 3.6e-284
Identity = 507/560 (90.54%), Postives = 523/560 (93.39%), Query Frame = 0

Query: 14  DVSRQEIQAAIAKAVELRALHAALMQGSSPSNLRFPSASPVSHAASQFSAQDYPVFTPSY 73
           DVSRQEIQAAIAKAVELRALHAALMQGSSPSNLRFPS SPV    SQFSAQDYPVFTPSY
Sbjct: 15  DVSRQEIQAAIAKAVELRALHAALMQGSSPSNLRFPSPSPV----SQFSAQDYPVFTPSY 74

Query: 74  EDEPTNGYHQVAMRIGTFSESWDDYGL----GGGNEDEAVLSDYRKEISKGLASDLTNLE 133
           EDEPTNGYHQV MRIGTFSESWD+YGL    GGGN+DE VLSDY+KEISKGL SD TNLE
Sbjct: 75  EDEPTNGYHQVPMRIGTFSESWDEYGLGGGGGGGNDDETVLSDYKKEISKGLVSDFTNLE 134

Query: 134 PHICPAEDHKSLTGSALQTSPANDYFKSSRRNSLGDLKSASSCNRCRPATITKENDNTAK 193
           PHICPAEDHK LTG + QTSPANDYFKSSRRNSLGD KS SSCNRCRPA ITKE DNTAK
Sbjct: 135 PHICPAEDHKPLTGFSFQTSPANDYFKSSRRNSLGDSKSVSSCNRCRPAIITKETDNTAK 194

Query: 194 NSRLSNTVVPLTDSHFATQSQPKSRGVISWLFPRLKKKNKNENSPNRTESEDVSQIFKDF 253
           NSRLSNT+VPLTDSH +TQSQPKSRGVIS LFPRLKKKNK ENSPNRTESEDVSQIFKD 
Sbjct: 195 NSRLSNTIVPLTDSHLSTQSQPKSRGVISRLFPRLKKKNKYENSPNRTESEDVSQIFKDL 254

Query: 254 GIVSIETLKKELIEANETRDAALMEVSEMKSSLGELRQKLEALETYCEELKRALKQATQA 313
           GIVSIETLKKELI ANETRDAALMEVSEMK+SLGELRQKLE LE YCEELKRALKQAT A
Sbjct: 255 GIVSIETLKKELINANETRDAALMEVSEMKTSLGELRQKLEGLENYCEELKRALKQATNA 314

Query: 314 RDLQTPTNFPKRITLSAGTNGENRMPVSEEVMVEGFLQIVSEARLSVKQFCKTLVSQIEE 373
           RDLQT TN  KRIT ++G N ENRMPVSEEVMVEGFLQIVSEARLSVKQFCKTLVSQIEE
Sbjct: 315 RDLQTATNLHKRITSTSGMNEENRMPVSEEVMVEGFLQIVSEARLSVKQFCKTLVSQIEE 374

Query: 374 TDSTLMENLNSLLQPYKLSLNSKYSRAVLYHLEAIINQALYQDFENCVFQKNGSPKLLDP 433
           TD+TLMENLN +LQPYKLSLNSKYSRAVLYHLEAIINQALYQDFENCVFQKNGSPKLLDP
Sbjct: 375 TDNTLMENLNLILQPYKLSLNSKYSRAVLYHLEAIINQALYQDFENCVFQKNGSPKLLDP 434

Query: 434 LQDRQAQFSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIITTLNWTRPWPEQLL 493
            QDRQAQFSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIITTLNWTRPWPEQLL
Sbjct: 435 HQDRQAQFSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIITTLNWTRPWPEQLL 494

Query: 494 QAFFVAAKCIWLLHLLAFSFDPPLKILRVEDNRSFDSSYMEDMFAERQRNGPSRVKIMVM 553
           QAFFVA KCIWLLHLLAFSFDPPLKILRVE+NRSFDSSYM+D+FAERQ+NGPSRVKIMVM
Sbjct: 495 QAFFVAGKCIWLLHLLAFSFDPPLKILRVEENRSFDSSYMDDVFAERQKNGPSRVKIMVM 554

Query: 554 PGFYVQDRILRCKVVCRYKS 570
           PGFYVQD+ILRCKVVCRYKS
Sbjct: 555 PGFYVQDKILRCKVVCRYKS 570

BLAST of Sgr014685 vs. ExPASy TrEMBL
Match: A0A5D3B8I3 (IRK-interacting protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold182G001300 PE=4 SV=1)

HSP 1 Score: 976.1 bits (2522), Expect = 6.4e-281
Identity = 504/562 (89.68%), Postives = 520/562 (92.53%), Query Frame = 0

Query: 14  DVSRQEIQAAIAKAVELRALHAALMQGSSPSNLRFPSASPVSHAASQFSAQDYPVFTPSY 73
           DVSRQEIQAAIAKAVELRALHAALMQGSSPSNLRFPSASP+    SQFSAQDYPVFTPSY
Sbjct: 15  DVSRQEIQAAIAKAVELRALHAALMQGSSPSNLRFPSASPI----SQFSAQDYPVFTPSY 74

Query: 74  EDEPTNGYHQVAMRIGTFSESWDDYGL------GGGNEDEAVLSDYRKEISKGLASDLTN 133
           EDEPTNGYHQV MRIGTFSESWD+YGL      GGGNEDE VLSDY+KEISK    D TN
Sbjct: 75  EDEPTNGYHQVPMRIGTFSESWDEYGLGGGGGGGGGNEDETVLSDYKKEISK----DFTN 134

Query: 134 LEPHICPAEDHKSLTGSALQTSPANDYFKSSRRNSLGDLKSASSCNRCRPATITKENDNT 193
           LEPHICPAEDHK LTG A Q+SP NDYFKSSRRNSLGD KS SSCNRCRPA ITKE DNT
Sbjct: 135 LEPHICPAEDHKHLTGHAFQSSPVNDYFKSSRRNSLGDSKSVSSCNRCRPAIITKETDNT 194

Query: 194 AKNSRLSNTVVPLTDSHFATQSQPKSRGVISWLFPRLKKKNKNENSPNRTESEDVSQIFK 253
           AKNSRLSNT+VPLTDSH +TQSQPKSRGVIS LFPRLKKKNK ENSPNRTESEDVSQIFK
Sbjct: 195 AKNSRLSNTIVPLTDSHLSTQSQPKSRGVISRLFPRLKKKNKYENSPNRTESEDVSQIFK 254

Query: 254 DFGIVSIETLKKELIEANETRDAALMEVSEMKSSLGELRQKLEALETYCEELKRALKQAT 313
           D GIVSIETLKKELI ANETRDAALMEVSEMK+SLGELRQKLE LE YCEELKRALKQAT
Sbjct: 255 DLGIVSIETLKKELINANETRDAALMEVSEMKTSLGELRQKLEGLENYCEELKRALKQAT 314

Query: 314 QARDLQTPTNFPKRITLSAGTNGENRMPVSEEVMVEGFLQIVSEARLSVKQFCKTLVSQI 373
            ARDLQT TN  KR T +AG N ENRMPVSEEVMVEGFLQIVSEARLSVKQFCKTLVSQI
Sbjct: 315 NARDLQTATNLHKRTTSTAGMNEENRMPVSEEVMVEGFLQIVSEARLSVKQFCKTLVSQI 374

Query: 374 EETDSTLMENLNSLLQPYKLSLNSKYSRAVLYHLEAIINQALYQDFENCVFQKNGSPKLL 433
           EETD+TLMENLNS+LQPYKLSLNSKYSRAVLYHLEAIINQALYQDFENCVFQKNGSPKLL
Sbjct: 375 EETDNTLMENLNSILQPYKLSLNSKYSRAVLYHLEAIINQALYQDFENCVFQKNGSPKLL 434

Query: 434 DPLQDRQAQFSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIITTLNWTRPWPEQ 493
           DP QDRQAQFSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIITTLNWTRPWPEQ
Sbjct: 435 DPHQDRQAQFSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIITTLNWTRPWPEQ 494

Query: 494 LLQAFFVAAKCIWLLHLLAFSFDPPLKILRVEDNRSFDSSYMEDMFAERQRNGPSRVKIM 553
           LLQAFFVA KCIWLLHLLAFSFDPPLKILRVE+NRSFDSSYM+D+FAERQ+NGPSRVKIM
Sbjct: 495 LLQAFFVAGKCIWLLHLLAFSFDPPLKILRVEENRSFDSSYMDDVFAERQKNGPSRVKIM 554

Query: 554 VMPGFYVQDRILRCKVVCRYKS 570
           VMPGFYVQ++ILRCKVVCRYKS
Sbjct: 555 VMPGFYVQEKILRCKVVCRYKS 568

BLAST of Sgr014685 vs. TAIR 10
Match: AT5G12900.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 21 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G12330.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 576.6 bits (1485), Expect = 2.2e-164
Identity = 332/571 (58.14%), Postives = 410/571 (71.80%), Query Frame = 0

Query: 18  QEIQAAIAKAVELRALHAALMQ-GSSPS---NLRFPS----ASPVSHAASQFSAQDYPVF 77
           +E + AIA  V+LRAL A+LMQ  SSPS   +LR PS    ASP S      SA DYPVF
Sbjct: 12  REKEEAIANGVKLRALQASLMQMKSSPSSNYSLRNPSSSSAASPASRPLPNLSAHDYPVF 71

Query: 78  TPSYEDEPTNGYHQVAMRIGTFSESWDDYGLGGGNEDEAVLSDYRKEISKGLASDLTNLE 137
           TPSYEDEP + +H   +   T SE+WD+ G+G  + D  +   Y+   S+      T + 
Sbjct: 72  TPSYEDEPVSAFHHKNL---TLSETWDEDGVGLVDGDTYLSDSYKTSTSR-----KTVMM 131

Query: 138 PHICPAEDHKSLTGSALQTSPANDYFKSSRRNSLGDLKSASSCNRCRPATITKENDNTAK 197
           PH   +  H      AL++ P + Y          D +S SSCN        K+     K
Sbjct: 132 PH-QDSHHHVYTMSDALRSPPLHFYTTGRSNCGSVDFRSVSSCN-----DYNKQKGFDTK 191

Query: 198 NSRLSNTVVPLTDSHFA-TQSQPKSRG--VISWLFPRLKKKNKNE---NSPNRTE-SEDV 257
           + + SN VVPLTDSH A   SQP++RG  V+SWLFP+LKKK K+    NSP+ TE SE+V
Sbjct: 192 SLKSSNLVVPLTDSHSAVVSSQPRNRGGRVMSWLFPKLKKKQKSNSIFNSPSITEKSEEV 251

Query: 258 SQIFKDFGIVSIETLKKELIEANETRDAALMEVSEMKSSLGELRQKLEALETYCEELKRA 317
           S++ KD G   +E LK+EL+EAN +RDAAL +VSEMKSSLGEL +KL+ LE+YC+ LK+A
Sbjct: 252 SEVLKDSG-SGVEKLKRELMEANRSRDAALTQVSEMKSSLGELSEKLQYLESYCDNLKKA 311

Query: 318 LKQATQARDLQTPTNFPKRITLSAGTNGENRMPVSEEVMVEGFLQIVSEARLSVKQFCKT 377
           L++AT+    +           S+G    + MPVSEEVMVEGFLQIVSEARLS+KQF KT
Sbjct: 312 LREATEVVSQENSGG------RSSGKK-NSEMPVSEEVMVEGFLQIVSEARLSIKQFLKT 371

Query: 378 LVSQIEETDSTLMENLNSLLQPYKLSLNSKYSRAVLYHLEAIINQALYQDFENCVFQKNG 437
           LVS+I+E DSTL+ N+N+LLQP+ LS  SKYS+ + YHLEAII+Q++YQDFENCVFQKNG
Sbjct: 372 LVSEIDEEDSTLIGNINTLLQPHNLSFTSKYSKIIQYHLEAIISQSVYQDFENCVFQKNG 431

Query: 438 SPKLLDPLQDRQAQFSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIITTLNWTR 497
            PKLLDP QDRQA FSSF +LRNLSWNEVL+KGTKYYS+EFS+FCD+KMS IITTLNWTR
Sbjct: 432 KPKLLDPEQDRQANFSSFASLRNLSWNEVLKKGTKYYSDEFSRFCDEKMSLIITTLNWTR 491

Query: 498 PWPEQLLQAFFVAAKCIWLLHLLAFSFDPPLKILRVEDNRSFDSSYMEDMFAERQRN--- 557
           PW EQ+LQAFFVAAKC+WLLHLLAFSF+P L ILRVE+NR F+SS+MEDM A+RQR+   
Sbjct: 492 PWSEQMLQAFFVAAKCVWLLHLLAFSFNPALGILRVEENREFESSFMEDMGADRQRSALS 551

Query: 558 -GPSRVKIMVMPGFYVQDRILRCKVVCRYKS 570
            GP+RVK+MVMPGFYV DR+LRCKV+CRYKS
Sbjct: 552 RGPARVKVMVMPGFYVLDRVLRCKVLCRYKS 560

BLAST of Sgr014685 vs. TAIR 10
Match: AT1G12330.1 (unknown protein; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 21 plant structures; EXPRESSED DURING: 12 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G12900.1); Has 249 Blast hits to 249 proteins in 27 species: Archae - 0; Bacteria - 0; Metazoa - 7; Fungi - 14; Plants - 217; Viruses - 0; Other Eukaryotes - 11 (source: NCBI BLink). )

HSP 1 Score: 248.4 bits (633), Expect = 1.3e-65
Identity = 149/406 (36.70%), Postives = 239/406 (58.87%), Query Frame = 0

Query: 169 ASSCNRCRPATITKENDNTAKNSRLSNTVVPLTDSHFATQSQPKSRGVISWLFPRLKKKN 228
           A SCN+CRP            + R   +VVPL +SH           +I  +F  L +++
Sbjct: 98  AVSCNKCRP----------HHSHRDKFSVVPL-ESHNNPSFISSPNLIIKSIFQSLTRRS 157

Query: 229 KNENS------PNRTESEDVSQIFKDFGIVSIETLKKELIEANETRDAALMEVSEMKSSL 288
              +S      P  + S       ++   +++  L  +LI+A + ++ A++E S +KSS+
Sbjct: 158 PKPSSATAALPPRSSSSSAADASREEQWRLAVAELSHKLIQATKKKEDAVIEASRLKSSM 217

Query: 289 GELRQKLEALETYCEELKRALKQATQARDLQTPTNFPKRITLSAGTNGENRMPVSEEVMV 348
            EL +KL  LE YC  LK  L + +            K+ ++    +G N      + ++
Sbjct: 218 AELEKKLNKLEIYCHNLKSGLDECSN-----------KKQSVPIRKDGFN------DRII 277

Query: 349 EGFLQIVSEARLSVKQFCKTLVSQIEETDSTLMENLNSLLQPYKLSLNS--KYSRAVLYH 408
           + FL  VSE+R S++   ++L SQ+      + E L+ LLQP+ + +NS  K  ++++++
Sbjct: 278 QQFLVSVSESRSSIRALSRSLASQLRTVGGKVYERLSLLLQPFDVKINSFAKNPKSLIFY 337

Query: 409 LEAIINQALYQDFENCVFQKNGSPKLLDPLQDRQAQFSSFVALRNLSWNEVLRKGTKYYS 468
           LEAI+++A ++DFE   FQKNGS ++L+P+   ++ ++SF  L  L+W+EVL +GTK++S
Sbjct: 338 LEAILSRAFFEDFEAPGFQKNGSTRILNPIDRCESNYASFNVLMELTWDEVLSRGTKHFS 397

Query: 469 EEFSKFCDQKMSCIITTLNWTRPWPEQLLQAFFVAAKCIWLLHLLAFSFDPPLKILRVED 528
           EEFS+FCD+KMS +++ L+W R WPE LLQAFF A+K +WL+HLLA S +P L+I RVE 
Sbjct: 398 EEFSRFCDRKMSDVVSMLSWNRAWPEPLLQAFFGASKSVWLVHLLANSVNPGLQIFRVEK 457

Query: 529 NRSFDSSYMEDMFAERQRNGPSRVKIMVMPGFYVQDRILRCKVVCR 567
           +  FD  YME+   ER +   S V+ MV PGFYV   +++CKVVC+
Sbjct: 458 DDRFDPIYMEETGGERFK---SLVRAMVQPGFYVYGSVVKCKVVCK 472

BLAST of Sgr014685 vs. TAIR 10
Match: AT2G45260.1 (Plant protein of unknown function (DUF641) )

HSP 1 Score: 75.5 bits (184), Expect = 1.6e-13
Identity = 83/335 (24.78%), Postives = 147/335 (43.88%), Query Frame = 0

Query: 258 KKELIEANETRDAALMEVSEMKSSL-------GELRQKLEALETYCEELKRALKQATQAR 317
           K   +   ++R AA  E+ E +S L        + + +++  ++   ++ + +++A Q R
Sbjct: 96  KPVCVSPQDSRLAA--EIQEQQSLLKTYEVMVKKFQSEIQNKDSEITQMLQKIEEANQKR 155

Query: 318 DLQTPTNFPKR-ITLSAGTNGENRMPVSEEVMVEGFLQIVSEARLSVKQFCKTLVSQIEE 377
            L+   N   R ++ + G+NG+  M    ++  E ++     A  +V  F K L++ ++ 
Sbjct: 156 -LKLEKNLKLRGMSTNEGSNGDGNMQF-PDLTTELYVSTYEAAAKAVHDFSKPLINMMKA 215

Query: 378 TDSTLMENLNSLLQPYKLSLNSKYSRAVLYHLEAIINQALYQDFENCVFQKNGSPKLLDP 437
               L    NS ++P  +     + +   Y  E+ I Q ++  F+   F  N     +  
Sbjct: 216 AGWDLDSAANS-IEPDVVYAKRPHKK---YAFESYICQRMFSGFQQKNFSVNSESAAVMA 275

Query: 438 LQDRQAQFSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCII-------------- 497
             D    F  F+AL+++   + L  GT   S  F  FC  K   ++              
Sbjct: 276 NDDTDTFFRQFLALKDMDPLDAL--GTNPDS-NFGIFCRSKYLLLVHPKMEASFFGNLDQ 335

Query: 498 --TTLNWTRPWPEQLLQAFFVAAKCIWLLHLLAFSFDPPLKILRVEDNRSFDSSYMEDMF 557
                    P      QAF   AK IW+LH LA+SFDP  KI +V+    F  SYME + 
Sbjct: 336 RDYVTGGGHP-RTAFYQAFLKLAKSIWILHRLAYSFDPAAKIFQVKKGSEFSDSYMESVV 395

Query: 558 A-----ERQRNGPSRVKIMVMPGFYVQDRILRCKV 564
                 E++ N   RV +MVMPGF++   +++ +V
Sbjct: 396 KNIVVDEKEEN--PRVGLMVMPGFWIGGSVIQSRV 416

BLAST of Sgr014685 vs. TAIR 10
Match: AT3G14870.1 (Plant protein of unknown function (DUF641) )

HSP 1 Score: 56.6 bits (135), Expect = 7.5e-08
Identity = 86/339 (25.37%), Postives = 133/339 (39.23%), Query Frame = 0

Query: 257 LKKELIEANETRDAALMEVSEMKSSLGE---LRQKLEALETYCEELKRALKQATQARDLQ 316
           LKK+L + N  R   L E+ E++S L     + +KLE      +     LK+  Q    Q
Sbjct: 140 LKKQL-DPNPDRTLVLAEIQELRSVLKTYEIMGKKLECQLKLKDSEIIFLKEKFQESMTQ 199

Query: 317 TPTNFPKRITLSAGTNGENRMPVSEEVMVEG-----FLQIVSEARLSVKQFCKTLVSQIE 376
                 KR+  S    G+   P+   + +       F+  +     S++ F K +V Q+ 
Sbjct: 200 NKL-MEKRLNQS----GQLCNPLDHNLHLSAVSSTHFVTYLHHTVKSIRGFVKLMVEQM- 259

Query: 377 ETDSTLMENLNSLLQPYKLSLNSKYSRAVLYHLEAIINQALYQDFENCVFQKNGSPKLLD 436
           +  +  ++    L+QP  L     +     + LE  + + + + F+   F    S K   
Sbjct: 260 KLAAWDIDMAAELIQPDVLYYKQDHK---CFALEHYVCKIMLEAFQLPYFSNESSKK--T 319

Query: 437 PLQDRQAQFSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCII------------- 496
             +D+   F  F  LR++   E L    K      +KFC  K   +I             
Sbjct: 320 SREDKAMFFERFTELRSMKPREYLASRPK---SRLAKFCRTKYLQLIHPKMEQAFFGHLH 379

Query: 497 -TTLNWTRPWPE-QLLQAFFVAAKCIWLLHLLAFSFDPPLKILRVEDNRSFDSSYM---- 556
                    +PE  L  AF   AK +WLLH LAFSFDP   I +V     F   YM    
Sbjct: 380 QRNQVTAGEFPETSLCTAFLEMAKRVWLLHCLAFSFDPEASIFQVSRGCRFSEVYMKSVS 439

Query: 557 -EDMFAERQRNGPSR----VKIMVMPGFYVQDRILRCKV 564
            E  F+  Q    S     V   V+PGF +    ++C+V
Sbjct: 440 EEAFFSPEQEESSSETEPGVAFTVVPGFRIGKTTIQCEV 463

BLAST of Sgr014685 vs. TAIR 10
Match: AT3G14870.2 (Plant protein of unknown function (DUF641) )

HSP 1 Score: 56.6 bits (135), Expect = 7.5e-08
Identity = 86/339 (25.37%), Postives = 133/339 (39.23%), Query Frame = 0

Query: 257 LKKELIEANETRDAALMEVSEMKSSLGE---LRQKLEALETYCEELKRALKQATQARDLQ 316
           LKK+L + N  R   L E+ E++S L     + +KLE      +     LK+  Q    Q
Sbjct: 136 LKKQL-DPNPDRTLVLAEIQELRSVLKTYEIMGKKLECQLKLKDSEIIFLKEKFQESMTQ 195

Query: 317 TPTNFPKRITLSAGTNGENRMPVSEEVMVEG-----FLQIVSEARLSVKQFCKTLVSQIE 376
                 KR+  S    G+   P+   + +       F+  +     S++ F K +V Q+ 
Sbjct: 196 NKL-MEKRLNQS----GQLCNPLDHNLHLSAVSSTHFVTYLHHTVKSIRGFVKLMVEQM- 255

Query: 377 ETDSTLMENLNSLLQPYKLSLNSKYSRAVLYHLEAIINQALYQDFENCVFQKNGSPKLLD 436
           +  +  ++    L+QP  L     +     + LE  + + + + F+   F    S K   
Sbjct: 256 KLAAWDIDMAAELIQPDVLYYKQDHK---CFALEHYVCKIMLEAFQLPYFSNESSKK--T 315

Query: 437 PLQDRQAQFSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCII------------- 496
             +D+   F  F  LR++   E L    K      +KFC  K   +I             
Sbjct: 316 SREDKAMFFERFTELRSMKPREYLASRPK---SRLAKFCRTKYLQLIHPKMEQAFFGHLH 375

Query: 497 -TTLNWTRPWPE-QLLQAFFVAAKCIWLLHLLAFSFDPPLKILRVEDNRSFDSSYM---- 556
                    +PE  L  AF   AK +WLLH LAFSFDP   I +V     F   YM    
Sbjct: 376 QRNQVTAGEFPETSLCTAFLEMAKRVWLLHCLAFSFDPEASIFQVSRGCRFSEVYMKSVS 435

Query: 557 -EDMFAERQRNGPSR----VKIMVMPGFYVQDRILRCKV 564
            E  F+  Q    S     V   V+PGF +    ++C+V
Sbjct: 436 EEAFFSPEQEESSSETEPGVAFTVVPGFRIGKTTIQCEV 459

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022148811.11.7e-29692.17IRK-interacting protein [Momordica charantia][more]
XP_023534028.12.9e-29691.43IRK-interacting protein [Cucurbita pepo subsp. pepo][more]
XP_022958158.15.0e-29691.43IRK-interacting protein [Cucurbita moschata][more]
KAG7035839.18.5e-29691.43IRK-interacting protein, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022995055.19.4e-29591.08IRK-interacting protein [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9LXU93.0e-16358.14IRK-interacting protein OS=Arabidopsis thaliana OX=3702 GN=IRKI PE=1 SV=1[more]
F4KGE85.3e-0623.70Protein GRAVITROPIC IN THE LIGHT 1 OS=Arabidopsis thaliana OX=3702 GN=GIL1 PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A6J1D6H98.3e-29792.17IRK-interacting protein OS=Momordica charantia OX=3673 GN=LOC111017373 PE=4 SV=1[more]
A0A6J1H2D12.4e-29691.43IRK-interacting protein OS=Cucurbita moschata OX=3662 GN=LOC111459468 PE=4 SV=1[more]
A0A6J1K3204.5e-29591.08IRK-interacting protein OS=Cucurbita maxima OX=3661 GN=LOC111490720 PE=4 SV=1[more]
A0A0A0KZA93.6e-28490.54Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G290210 PE=4 SV=1[more]
A0A5D3B8I36.4e-28189.68IRK-interacting protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
Match NameE-valueIdentityDescription
AT5G12900.12.2e-16458.14unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
AT1G12330.11.3e-6536.70unknown protein; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplas... [more]
AT2G45260.11.6e-1324.78Plant protein of unknown function (DUF641) [more]
AT3G14870.17.5e-0825.37Plant protein of unknown function (DUF641) [more]
AT3G14870.27.5e-0825.37Plant protein of unknown function (DUF641) [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 254..312
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 142..171
NoneNo IPR availablePANTHERPTHR31029:SF3IRK-INTERACTING PROTEINcoord: 10..569
IPR042316IRK-interacting protein-likePANTHERPTHR31029CYCLIN-DEPENDENT KINASE-LIKE PROTEINcoord: 10..569

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr014685.1Sgr014685.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006457 protein folding