Sgr014428 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr014428 vs. NCBI nr
Match: XP_022157299.1 (uncharacterized protein LOC111024031 [Momordica charantia])

HSP 1 Score: 1614.4 bits (4179), Expect = 0.0e+00
Identity = 829/956 (86.72%), Postives = 867/956 (90.69%), Query Frame = 0

Query: 44  LSKAVKPRASSFARTLSSSVLRLSLDLNHHTRRLRFPQIRLPRSAAKVVAKDGVAEMAVT 103
           LSK VKPRASSFA TLSSS LR S+D NHHT RLRFP+IR PR+AAKVVAK+GVAEMAVT
Sbjct: 13  LSKTVKPRASSFALTLSSSFLRSSVDSNHHTSRLRFPEIRFPRAAAKVVAKEGVAEMAVT 72

Query: 104 ELEREKQ----------SPSAERGISLNSARSVLDHIQGDDLQVSCYYIDSELNAYAISS 163
           ELERE +           PSAERGISLNSARSVLDHIQGDDL VSCYYID ELNAYAISS
Sbjct: 73  ELERENRGVLRVGLICGGPSAERGISLNSARSVLDHIQGDDLLVSCYYIDPELNAYAISS 132

Query: 164 AQVYSNTPADFDFKLKSLAHGFRSLTDFAEHLSASVDIVFPVIHGRFGEDGGIQELLERH 223
           AQVYSNTPADFDFKLKSLAHGF+SLTDFAEHLSASVDIVFPVIHGRFGEDGGIQELLERH
Sbjct: 133 AQVYSNTPADFDFKLKSLAHGFQSLTDFAEHLSASVDIVFPVIHGRFGEDGGIQELLERH 192

Query: 224 NIPFVGTGSTECSRAFDKYNASLELDRLGFITVPNFLVQAGSVNESELSQWFLRNQLDLS 283
           NIPFVGTGSTECS AFDKYNASLELD+LGFI VPNFL+QAGSV+ESELS+WFLRNQ+D S
Sbjct: 193 NIPFVGTGSTECSHAFDKYNASLELDKLGFIKVPNFLIQAGSVSESELSKWFLRNQIDPS 252

Query: 284 SGKVVVKPVRAGSSIGVTVAYGVVDSLKKANEIISKEIDDKVLVEVFLEGGSEFTAIVLD 343
            GKVVVKPVRAGSSIGVTVAYGV DSLKKA EIIS+EIDDKVLVEVFLEGGSEFTAIVLD
Sbjct: 253 FGKVVVKPVRAGSSIGVTVAYGVADSLKKAEEIISQEIDDKVLVEVFLEGGSEFTAIVLD 312

Query: 344 VGSGSVCHPVVLLPTEVELQFMVRLMWERRIQFSIIGGNIFPPSRYSDSVVPPILACLVA 403
           VGSGSVCHPVVLLPTEVELQF     W    +   I         Y    +P      V 
Sbjct: 313 VGSGSVCHPVVLLPTEVELQFH---GWADAGEKDAI-------FNYRRKYLP---TQQVT 372

Query: 404 YHTPPRFPVDVIESIREGASLLFKGLGLRDFARIDGWFLPNCCHESSYSTGNFGRTESGT 463
           YHTPPRF VDVIESIR+GASLLFKGLGLRDFARIDGWFLPN   ESS S G FGRTESGT
Sbjct: 373 YHTPPRFAVDVIESIRKGASLLFKGLGLRDFARIDGWFLPNSSRESSCSVGKFGRTESGT 432

Query: 464 VIYTDINLISGMEQTSFLFQQASKVGFSHSNVLRSIIYHACLRYPSLGSLDHMFGNWPRR 523
           ++YTDINLISGMEQTSFLFQQASKVGFSHSN+LRSIIYHACLRYP LGSLD M GN  RR
Sbjct: 433 IVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYPDLGSLDCMSGNLLRR 492

Query: 524 SNSSQRSQAYSSRESLRKVFVIFGGDTSERQVSLMSGTNVWLNLQVFDELEVTPCLLAPT 583
           SNS+QRSQAYSS ES+RKVFVIFGGDTSERQVSLMSGTNVWLNLQVFDELEVTPCLLAP+
Sbjct: 493 SNSAQRSQAYSSHESIRKVFVIFGGDTSERQVSLMSGTNVWLNLQVFDELEVTPCLLAPS 552

Query: 584 NEQTSSVDLDKNEVDVSSRTVWSLPYSLVLRHTTEEVLAACIEAIEPTRAALTSRMRGLV 643
           NEQTSS+DLDKN+VD +SRTVWSLPYSLVLRHTTEEVLAACIEA+EPTRAALTSR+R LV
Sbjct: 553 NEQTSSLDLDKNQVDETSRTVWSLPYSLVLRHTTEEVLAACIEAMEPTRAALTSRLRELV 612

Query: 644 VADLKEGLKKHSWFAGFDIKDELPVRFSLEQWIKQAKEVDATVFIAVHGGIGEDGSLQSL 703
           + DLKEGLK H WFAGFDIKDELPVR+SLEQWIKQAKE DATVFIAVHGGIGEDG+LQSL
Sbjct: 613 MTDLKEGLKNHRWFAGFDIKDELPVRYSLEQWIKQAKEADATVFIAVHGGIGEDGTLQSL 672

Query: 704 LEAKGVPYTGPGVVASSICMDKVATSLALNHLSDMGVLTIKKDVRRKDDLLDIPIINVWH 763
           LEAKGVPYTGPG  AS+ICMDKVATSLALNHLSDMGVLTIKKDVRRKDDLL IPI+NVWH
Sbjct: 673 LEAKGVPYTGPGAAASNICMDKVATSLALNHLSDMGVLTIKKDVRRKDDLLQIPILNVWH 732

Query: 764 DLTCKLQCQSLCVKPARDGCSTGVARLCCADDLAVYVKALKDCLARIPSNSFSKAHGMIE 823
           DLT KL CQSLCVKPARDGCSTGVARLCCADDLAVYVKAL+DCL RIPSNS SKAHGMIE
Sbjct: 733 DLTRKLHCQSLCVKPARDGCSTGVARLCCADDLAVYVKALEDCLVRIPSNSLSKAHGMIE 792

Query: 824 MPKPPPELLICEPFIETDEIIVSSKSTNETEERLLWKGQSRWVEITVGVVGTRGSMHSLS 883
           MPKPPPELLI EPFIETDEIIVSSKSTNET+E+LLW+GQSRWVEITVGVVGTRGSMHSLS
Sbjct: 793 MPKPPPELLIFEPFIETDEIIVSSKSTNETKEQLLWEGQSRWVEITVGVVGTRGSMHSLS 852

Query: 884 PSVTVKEHGDILSLEEKFQGGTGINLTPPPLSIISNEALEKCKQHIELISNTLQLEGFSR 943
           PSVTVKE GDILSLEEKFQGGTGINLTPPPL II NEALEKCK+HIELI+NTLQLEGFSR
Sbjct: 853 PSVTVKESGDILSLEEKFQGGTGINLTPPPLMIIRNEALEKCKRHIELIANTLQLEGFSR 912

Query: 944 IDAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQALAETPPVYPHQFFRRLLDLASER 990
           IDAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQALAETPPVYPHQFFRRLL+LASER
Sbjct: 913 IDAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQALAETPPVYPHQFFRRLLNLASER 955

BLAST of Sgr014428 vs. NCBI nr
Match: XP_022934689.1 (uncharacterized protein LOC111441803 [Cucurbita moschata] >XP_022934690.1 uncharacterized protein LOC111441803 [Cucurbita moschata] >XP_022934691.1 uncharacterized protein LOC111441803 [Cucurbita moschata] >XP_022934692.1 uncharacterized protein LOC111441803 [Cucurbita moschata] >KAG6580439.1 hypothetical protein SDJN03_20441, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1590.9 bits (4118), Expect = 0.0e+00
Identity = 821/960 (85.52%), Postives = 864/960 (90.00%), Query Frame = 0

Query: 44  LSKAVKPRASSFARTLSSSVLRLSLDLNHHTRRLRFPQIRLPRSAAKVVAKDGVAEMAVT 103
           L K +K RAS+F  TLSSS LRLSLDL++H RRL+ P+IR+ R+AAK+V KDGVAEMA T
Sbjct: 13  LCKDIKLRASNFTPTLSSSFLRLSLDLHNHARRLQSPKIRVSRAAAKMVVKDGVAEMATT 72

Query: 104 ELEREKQS--------------PSAERGISLNSARSVLDHIQGDDLQVSCYYIDSELNAY 163
           E+EREK S              PSAERGISLNSARSVLDHIQGDDLQVSCYYID ELNAY
Sbjct: 73  EIEREKHSVKCEALRVGLICGGPSAERGISLNSARSVLDHIQGDDLQVSCYYIDPELNAY 132

Query: 164 AISSAQVYSNTPADFDFKLKSLAHGFRSLTDFAEHLSASVDIVFPVIHGRFGEDGGIQEL 223
           AISSAQVYSNTPADFDFKLKSLA GFRSLTDFAEHLS SVDIVFPVIHGRFGEDGGIQEL
Sbjct: 133 AISSAQVYSNTPADFDFKLKSLARGFRSLTDFAEHLSTSVDIVFPVIHGRFGEDGGIQEL 192

Query: 224 LERHNIPFVGTGSTECSRAFDKYNASLELDRLGFITVPNFLVQAGSVNESELSQWFLRNQ 283
           LE HNIPFVGTGSTEC+ AFDKYNA+LELDRLGFITVPNFLVQAGSV+ES+LS+WFLRNQ
Sbjct: 193 LETHNIPFVGTGSTECAHAFDKYNAALELDRLGFITVPNFLVQAGSVSESDLSKWFLRNQ 252

Query: 284 LDLSSGKVVVKPVRAGSSIGVTVAYGVVDSLKKANEIISKEIDDKVLVEVFLEGGSEFTA 343
           LDLSSGKVVVKPVRAGSSIGV+VAYGVVDSLKKANEIISKEIDDKVLVEVFLE GSEFTA
Sbjct: 253 LDLSSGKVVVKPVRAGSSIGVSVAYGVVDSLKKANEIISKEIDDKVLVEVFLERGSEFTA 312

Query: 344 IVLDVGSGSVCHPVVLLPTEVELQFMVRLMWERRIQFSIIGGNIFPPSRYSDSVVPPILA 403
           IVLDVGSGSVCHPVVLLPTEVEL+      W    +   I         Y    +P    
Sbjct: 313 IVLDVGSGSVCHPVVLLPTEVELESHA---WADAGEKDAI-------FNYRRKYLP---T 372

Query: 404 CLVAYHTPPRFPVDVIESIREGASLLFKGLGLRDFARIDGWFLPNCCHESSYSTGNFGRT 463
             VAYHTPPRFP+DVIESIREGASLLFKGLGL DFARIDGWFLPN  H+SS STG FGRT
Sbjct: 373 QQVAYHTPPRFPIDVIESIREGASLLFKGLGLCDFARIDGWFLPNSSHDSSSSTGKFGRT 432

Query: 464 ESGTVIYTDINLISGMEQTSFLFQQASKVGFSHSNVLRSIIYHACLRYPSLGSLDHMFGN 523
            SGTV+YTDINLISGMEQTSFLFQQASKVGFSHSN+LRSIIYHACLRYPSLGSLD M GN
Sbjct: 433 ASGTVVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYPSLGSLDCMSGN 492

Query: 524 WPRRSNSSQRSQAYSSRESLRKVFVIFGGDTSERQVSLMSGTNVWLNLQVFDELEVTPCL 583
            PRRS SSQR+QAYSS ES +KVFVI GGDTSERQVSLMSGTNVWLNLQ FD+LEVTPCL
Sbjct: 493 MPRRSISSQRTQAYSSTESNQKVFVILGGDTSERQVSLMSGTNVWLNLQAFDDLEVTPCL 552

Query: 584 LAPTNEQTSSVDLDKNEVDVSSRTVWSLPYSLVLRHTTEEVLAACIEAIEPTRAALTSRM 643
           LAP+ EQTSSVDLDKNE D++SRTVWSLPYSLVLRHTTEEVLAACIEA EPTRAALTS +
Sbjct: 553 LAPSIEQTSSVDLDKNEADMTSRTVWSLPYSLVLRHTTEEVLAACIEAFEPTRAALTSHL 612

Query: 644 RGLVVADLKEGLKKHSWFAGFDIKDELPVRFSLEQWIKQAKEVDATVFIAVHGGIGEDGS 703
           R LVV DL+EGLKKHSWFAGFDIKDELPV+FSLEQWIK+AKEVDATVFIAVHGGIGE+G 
Sbjct: 613 RELVVTDLEEGLKKHSWFAGFDIKDELPVKFSLEQWIKKAKEVDATVFIAVHGGIGENGM 672

Query: 704 LQSLLEAKGVPYTGPGVVASSICMDKVATSLALNHLSDMGVLTIKKDVRRKDDLLDIPII 763
           LQSLLEAKGVPYTGPGV AS+ICMDKVATSLALNHLSDMGVLTIKKD RRKD+LL IP +
Sbjct: 673 LQSLLEAKGVPYTGPGVAASNICMDKVATSLALNHLSDMGVLTIKKDARRKDELLHIPTL 732

Query: 764 NVWHDLTCKLQCQSLCVKPARDGCSTGVARLCCADDLAVYVKALKDCLARIPSNSFSKAH 823
           N+WHDLTCKLQCQSLCVKPARDGCSTGVARLCCADDLAVYVKAL+DCL RIPSNS S+AH
Sbjct: 733 NLWHDLTCKLQCQSLCVKPARDGCSTGVARLCCADDLAVYVKALEDCLVRIPSNSLSRAH 792

Query: 824 GMIEMPKPPPELLICEPFIETDEIIVSSKSTNETEERLLWKGQSRWVEITVGVVGTRGSM 883
           GMIEMPKPPPELLI EPFIETDEIIVSSK TNE+ ERLLWKGQSRWVEITVGVVGTRGSM
Sbjct: 793 GMIEMPKPPPELLIFEPFIETDEIIVSSKYTNES-ERLLWKGQSRWVEITVGVVGTRGSM 852

Query: 884 HSLSPSVTVKEHGDILSLEEKFQGGTGINLTPPPLSIISNEALEKCKQHIELISNTLQLE 943
           HSLSPSVTVKE GDILSLEEKFQGGTGINLTPPPLSIISNE+LEKCKQHIELI+NTLQLE
Sbjct: 853 HSLSPSVTVKESGDILSLEEKFQGGTGINLTPPPLSIISNESLEKCKQHIELIANTLQLE 912

Query: 944 GFSRIDAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQALAETPPVYPHQFFRRLLDLASER 990
           GFSRIDAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQALAETPPVYPHQFFRRLLDLA ER
Sbjct: 913 GFSRIDAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQALAETPPVYPHQFFRRLLDLAWER 958

BLAST of Sgr014428 vs. NCBI nr
Match: XP_022982716.1 (uncharacterized protein LOC111481502 [Cucurbita maxima] >XP_022982717.1 uncharacterized protein LOC111481502 [Cucurbita maxima] >XP_022982718.1 uncharacterized protein LOC111481502 [Cucurbita maxima])

HSP 1 Score: 1588.5 bits (4112), Expect = 0.0e+00
Identity = 819/960 (85.31%), Postives = 863/960 (89.90%), Query Frame = 0

Query: 44  LSKAVKPRASSFARTLSSSVLRLSLDLNHHTRRLRFPQIRLPRSAAKVVAKDGVAEMAVT 103
           L K ++PRAS+F  TLSSS LRLSLDL++H RRL+ P+IR+ R+AAK+V KDGVAEMA T
Sbjct: 13  LCKDIEPRASNFTPTLSSSFLRLSLDLHNHARRLQSPKIRVSRAAAKMVVKDGVAEMAAT 72

Query: 104 ELEREKQS--------------PSAERGISLNSARSVLDHIQGDDLQVSCYYIDSELNAY 163
           E+EREK S              PSAERGISLNSARSVLDHIQGDDLQVSCYYID ELNAY
Sbjct: 73  EIEREKHSVKCEALRVGLICGGPSAERGISLNSARSVLDHIQGDDLQVSCYYIDPELNAY 132

Query: 164 AISSAQVYSNTPADFDFKLKSLAHGFRSLTDFAEHLSASVDIVFPVIHGRFGEDGGIQEL 223
           AISSAQVYSNTPADFDFKLKSLA GFRSLTDFAEHLS SVDIVFPVIHGRFGEDGGIQEL
Sbjct: 133 AISSAQVYSNTPADFDFKLKSLARGFRSLTDFAEHLSTSVDIVFPVIHGRFGEDGGIQEL 192

Query: 224 LERHNIPFVGTGSTECSRAFDKYNASLELDRLGFITVPNFLVQAGSVNESELSQWFLRNQ 283
           LE HNIPFVGTGSTEC+ AFDKYNASLELDRLGFITVPNFLVQAGSV+ES+LS+WFLRNQ
Sbjct: 193 LETHNIPFVGTGSTECAHAFDKYNASLELDRLGFITVPNFLVQAGSVSESDLSKWFLRNQ 252

Query: 284 LDLSSGKVVVKPVRAGSSIGVTVAYGVVDSLKKANEIISKEIDDKVLVEVFLEGGSEFTA 343
           LDLSSGKVVVKP RAGSSIGV+VAYGVVDSLKKANEIISKEIDDKVLVEVFLE GSEFTA
Sbjct: 253 LDLSSGKVVVKPARAGSSIGVSVAYGVVDSLKKANEIISKEIDDKVLVEVFLERGSEFTA 312

Query: 344 IVLDVGSGSVCHPVVLLPTEVELQFMVRLMWERRIQFSIIGGNIFPPSRYSDSVVPPILA 403
           IVLDVGSGSVCHPVVLLPTEVELQ      W    +   I         Y    +P    
Sbjct: 313 IVLDVGSGSVCHPVVLLPTEVELQ---SHGWADAGEKDAI-------FNYRRKYLP---T 372

Query: 404 CLVAYHTPPRFPVDVIESIREGASLLFKGLGLRDFARIDGWFLPNCCHESSYSTGNFGRT 463
             VAYHTPPRFP+DVIESIREGASLLFKGLGL DFARIDGWFLPN  H+SS STG FGRT
Sbjct: 373 QQVAYHTPPRFPIDVIESIREGASLLFKGLGLCDFARIDGWFLPNSSHDSSSSTGKFGRT 432

Query: 464 ESGTVIYTDINLISGMEQTSFLFQQASKVGFSHSNVLRSIIYHACLRYPSLGSLDHMFGN 523
            SGTV+YTDINLISGMEQTSFLFQQASKVGFSHSN+LRSIIYHACLRYPSLGSLD M GN
Sbjct: 433 ASGTVVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYPSLGSLDCMSGN 492

Query: 524 WPRRSNSSQRSQAYSSRESLRKVFVIFGGDTSERQVSLMSGTNVWLNLQVFDELEVTPCL 583
            PRRS SSQRSQAYSS ES +KVF+IFGGDTSERQVSLMSGTNVWLNLQ FD+LEVTPCL
Sbjct: 493 MPRRSISSQRSQAYSSTESNQKVFLIFGGDTSERQVSLMSGTNVWLNLQAFDDLEVTPCL 552

Query: 584 LAPTNEQTSSVDLDKNEVDVSSRTVWSLPYSLVLRHTTEEVLAACIEAIEPTRAALTSRM 643
           LAP+ EQTSSVDLDKNE D++S+TVWSLPYSLVLRHTTEEVLAACIEA EPTRAALTS +
Sbjct: 553 LAPSIEQTSSVDLDKNEADMTSKTVWSLPYSLVLRHTTEEVLAACIEAFEPTRAALTSHL 612

Query: 644 RGLVVADLKEGLKKHSWFAGFDIKDELPVRFSLEQWIKQAKEVDATVFIAVHGGIGEDGS 703
           R LVV DL+EGLKKHSWFAGFDIKDELPV+FSLEQWIK+AKEVDATVFIAVHGGIGE+G 
Sbjct: 613 RELVVTDLEEGLKKHSWFAGFDIKDELPVKFSLEQWIKKAKEVDATVFIAVHGGIGENGM 672

Query: 704 LQSLLEAKGVPYTGPGVVASSICMDKVATSLALNHLSDMGVLTIKKDVRRKDDLLDIPII 763
           LQSLLEAKGVPYTGPGV AS+ICMDKVATSLALNHLSDMGVLTIKKD RRKD+LL+IP +
Sbjct: 673 LQSLLEAKGVPYTGPGVAASNICMDKVATSLALNHLSDMGVLTIKKDARRKDELLNIPTL 732

Query: 764 NVWHDLTCKLQCQSLCVKPARDGCSTGVARLCCADDLAVYVKALKDCLARIPSNSFSKAH 823
           N+WHDLTCKLQCQSLCVKPARDGCSTGVARLCCADDLAVY KAL+ CL RIPSNS S+AH
Sbjct: 733 NLWHDLTCKLQCQSLCVKPARDGCSTGVARLCCADDLAVYAKALEYCLVRIPSNSLSRAH 792

Query: 824 GMIEMPKPPPELLICEPFIETDEIIVSSKSTNETEERLLWKGQSRWVEITVGVVGTRGSM 883
           GMIEMPKPPPELLI EPFIETDEIIVSSK TNE+ ERLLWKGQSRWVEITVGVVGTRGSM
Sbjct: 793 GMIEMPKPPPELLIFEPFIETDEIIVSSKYTNES-ERLLWKGQSRWVEITVGVVGTRGSM 852

Query: 884 HSLSPSVTVKEHGDILSLEEKFQGGTGINLTPPPLSIISNEALEKCKQHIELISNTLQLE 943
           HSLSPSVTVKE GDILSLEEKFQGGTGINLTPPPLSIISNE+LEKCKQHIELI+N LQLE
Sbjct: 853 HSLSPSVTVKESGDILSLEEKFQGGTGINLTPPPLSIISNESLEKCKQHIELIANALQLE 912

Query: 944 GFSRIDAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQALAETPPVYPHQFFRRLLDLASER 990
           GFSRIDAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQALAETPPVYPHQFFRRLLDLA ER
Sbjct: 913 GFSRIDAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQALAETPPVYPHQFFRRLLDLALER 958

BLAST of Sgr014428 vs. NCBI nr
Match: XP_023528547.1 (uncharacterized protein LOC111791436 [Cucurbita pepo subsp. pepo] >XP_023528548.1 uncharacterized protein LOC111791436 [Cucurbita pepo subsp. pepo] >XP_023528549.1 uncharacterized protein LOC111791436 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1588.2 bits (4111), Expect = 0.0e+00
Identity = 821/960 (85.52%), Postives = 862/960 (89.79%), Query Frame = 0

Query: 44  LSKAVKPRASSFARTLSSSVLRLSLDLNHHTRRLRFPQIRLPRSAAKVVAKDGVAEMAVT 103
           L K +K RAS+F  TLSSS L LSLDL++H  RL+ P+IR+ R+AAK+V KDGVAEMA T
Sbjct: 13  LCKDIKLRASNFTPTLSSSFLGLSLDLHNHAPRLQSPKIRVSRAAAKMVVKDGVAEMAAT 72

Query: 104 ELEREKQS--------------PSAERGISLNSARSVLDHIQGDDLQVSCYYIDSELNAY 163
           E+EREK S              PSAERGISLNSARSVLDHIQGDDLQVSCYYID ELNAY
Sbjct: 73  EIEREKHSVKCEALRVGLICGGPSAERGISLNSARSVLDHIQGDDLQVSCYYIDPELNAY 132

Query: 164 AISSAQVYSNTPADFDFKLKSLAHGFRSLTDFAEHLSASVDIVFPVIHGRFGEDGGIQEL 223
           AISSAQVYSNTPADFDFKLKSLA GFRSLTDFAEHLS SVDIVFPVIHGRFGEDGGIQEL
Sbjct: 133 AISSAQVYSNTPADFDFKLKSLARGFRSLTDFAEHLSTSVDIVFPVIHGRFGEDGGIQEL 192

Query: 224 LERHNIPFVGTGSTECSRAFDKYNASLELDRLGFITVPNFLVQAGSVNESELSQWFLRNQ 283
           LE HNIPFVGTGSTEC+ AFDKYNASLELDRLGFITVPNFLVQAGSV+ES+LS+WFLRNQ
Sbjct: 193 LETHNIPFVGTGSTECAHAFDKYNASLELDRLGFITVPNFLVQAGSVSESDLSKWFLRNQ 252

Query: 284 LDLSSGKVVVKPVRAGSSIGVTVAYGVVDSLKKANEIISKEIDDKVLVEVFLEGGSEFTA 343
           LDLSSGKVVVKPVRAGSSIGV+VAYGVVDSLKKANEIISKEIDDKVLVEVFLE GSEFTA
Sbjct: 253 LDLSSGKVVVKPVRAGSSIGVSVAYGVVDSLKKANEIISKEIDDKVLVEVFLERGSEFTA 312

Query: 344 IVLDVGSGSVCHPVVLLPTEVELQFMVRLMWERRIQFSIIGGNIFPPSRYSDSVVPPILA 403
           IVLDVGSGSVCHPVVLLPTEVELQ      W    +   I         Y    +P    
Sbjct: 313 IVLDVGSGSVCHPVVLLPTEVELQSHA---WADAGEKDAI-------FNYRRKYLP---T 372

Query: 404 CLVAYHTPPRFPVDVIESIREGASLLFKGLGLRDFARIDGWFLPNCCHESSYSTGNFGRT 463
             VAYHTPPRFP+DVIESIREGASLLFKGLGL DFARIDGWFLPN  H+SS STG FGRT
Sbjct: 373 QQVAYHTPPRFPIDVIESIREGASLLFKGLGLCDFARIDGWFLPNSSHDSSSSTGKFGRT 432

Query: 464 ESGTVIYTDINLISGMEQTSFLFQQASKVGFSHSNVLRSIIYHACLRYPSLGSLDHMFGN 523
            SGTV+YTDINLISGMEQTSFLFQQASKVGFSHSN+LRSIIYHACLRYPSLGSLD M GN
Sbjct: 433 ASGTVVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYPSLGSLDCMSGN 492

Query: 524 WPRRSNSSQRSQAYSSRESLRKVFVIFGGDTSERQVSLMSGTNVWLNLQVFDELEVTPCL 583
            PRRS SSQR+QAYSS ES +KVFVI GGDTSERQVSLMSGTNVWLNLQ FD+LEVTPCL
Sbjct: 493 MPRRSISSQRTQAYSSTESNQKVFVILGGDTSERQVSLMSGTNVWLNLQAFDDLEVTPCL 552

Query: 584 LAPTNEQTSSVDLDKNEVDVSSRTVWSLPYSLVLRHTTEEVLAACIEAIEPTRAALTSRM 643
           LAP+ EQTSSVDLDKNE D++SRTVWSLPYSLVLRHTTEEVLAACIEA EPTRAALTS +
Sbjct: 553 LAPSIEQTSSVDLDKNEADMTSRTVWSLPYSLVLRHTTEEVLAACIEAFEPTRAALTSHL 612

Query: 644 RGLVVADLKEGLKKHSWFAGFDIKDELPVRFSLEQWIKQAKEVDATVFIAVHGGIGEDGS 703
           R LVV DL+EGLKKHSWFAGFDIKDELPV+FSLEQWIK+AKEVDATVFIAVHGGIGE+G 
Sbjct: 613 RELVVTDLEEGLKKHSWFAGFDIKDELPVKFSLEQWIKKAKEVDATVFIAVHGGIGENGM 672

Query: 704 LQSLLEAKGVPYTGPGVVASSICMDKVATSLALNHLSDMGVLTIKKDVRRKDDLLDIPII 763
           LQSLLEAKGVPYTGPGV AS+ICMDKVATSLALNHLSDMGVLTIKKD RRKD+LL IP +
Sbjct: 673 LQSLLEAKGVPYTGPGVAASNICMDKVATSLALNHLSDMGVLTIKKDARRKDELLHIPTL 732

Query: 764 NVWHDLTCKLQCQSLCVKPARDGCSTGVARLCCADDLAVYVKALKDCLARIPSNSFSKAH 823
           N+WHDLTCKLQCQSLCVKPARDGCSTGVARLCCADDLAVYVKAL+DCL RIPSNS S+AH
Sbjct: 733 NLWHDLTCKLQCQSLCVKPARDGCSTGVARLCCADDLAVYVKALEDCLVRIPSNSLSRAH 792

Query: 824 GMIEMPKPPPELLICEPFIETDEIIVSSKSTNETEERLLWKGQSRWVEITVGVVGTRGSM 883
           GMIEMPKPPPELLI EPFIETDEIIVSSK TNE+ ERLLWKGQSRWVEITVGVVGTRGSM
Sbjct: 793 GMIEMPKPPPELLIFEPFIETDEIIVSSKYTNES-ERLLWKGQSRWVEITVGVVGTRGSM 852

Query: 884 HSLSPSVTVKEHGDILSLEEKFQGGTGINLTPPPLSIISNEALEKCKQHIELISNTLQLE 943
           HSLSPSVTVKE GDILSLEEKFQGGTGINLTPPPLSIISNE+LEKCKQHIELI+NTLQLE
Sbjct: 853 HSLSPSVTVKESGDILSLEEKFQGGTGINLTPPPLSIISNESLEKCKQHIELIANTLQLE 912

Query: 944 GFSRIDAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQALAETPPVYPHQFFRRLLDLASER 990
           GFSRIDAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQALAETPPVYPHQFFRRLLDLA ER
Sbjct: 913 GFSRIDAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQALAETPPVYPHQFFRRLLDLALER 958

BLAST of Sgr014428 vs. NCBI nr
Match: XP_038903726.1 (uncharacterized protein LOC120090245 isoform X1 [Benincasa hispida] >XP_038903727.1 uncharacterized protein LOC120090245 isoform X1 [Benincasa hispida] >XP_038903728.1 uncharacterized protein LOC120090245 isoform X1 [Benincasa hispida] >XP_038903729.1 uncharacterized protein LOC120090245 isoform X1 [Benincasa hispida])

HSP 1 Score: 1580.1 bits (4090), Expect = 0.0e+00
Identity = 818/962 (85.03%), Postives = 870/962 (90.44%), Query Frame = 0

Query: 45  SKAVKPRASSFARTLSSSVLRLSLDLNHHT--RRLRFPQIRLPRSAAKVVAKDGVAEMAV 104
           SKA+K RAS+FA TL SS LRLSLDLN++T  RRL+ P+I LPRSAAKV+AKDGVA+MAV
Sbjct: 14  SKALKLRASTFAPTLPSSFLRLSLDLNNYTRRRRLQSPKIHLPRSAAKVLAKDGVAQMAV 73

Query: 105 TELEREKQS--------------PSAERGISLNSARSVLDHIQGDDLQVSCYYIDSELNA 164
           T +ERE+QS              PSAERGISLNSARSVLDHIQGDDLQVSCYYIDSELNA
Sbjct: 74  TGMERERQSEKCGALRVGLICGGPSAERGISLNSARSVLDHIQGDDLQVSCYYIDSELNA 133

Query: 165 YAISSAQVYSNTPADFDFKLKSLAHGFRSLTDFAEHLSASVDIVFPVIHGRFGEDGGIQE 224
           YAISSAQVYSNTPADFDFKLKSLA GF SLT+FAEHLS SVDIVFPVIHGRFGEDGGIQE
Sbjct: 134 YAISSAQVYSNTPADFDFKLKSLARGFLSLTEFAEHLSTSVDIVFPVIHGRFGEDGGIQE 193

Query: 225 LLERHNIPFVGTGSTECSRAFDKYNASLELDRLGFITVPNFLVQAGSVNESELSQWFLRN 284
           LLE HNIPFVGTGST+CS AFDKYNASLELDRLGFITVPNFLVQAG V+ESELS+WFL N
Sbjct: 194 LLETHNIPFVGTGSTQCSHAFDKYNASLELDRLGFITVPNFLVQAGGVSESELSKWFLSN 253

Query: 285 QLDLSSGKVVVKPVRAGSSIGVTVAYGVVDSLKKANEIISKEIDDKVLVEVFLEGGSEFT 344
           QLDLSSGKVVVKP RAGSSIGV+VAYGVVDSLKKANEIIS EIDDKVLVEVFLEGGSEFT
Sbjct: 254 QLDLSSGKVVVKPARAGSSIGVSVAYGVVDSLKKANEIISMEIDDKVLVEVFLEGGSEFT 313

Query: 345 AIVLDVGSGSVCHPVVLLPTEVELQFM-VRLMWERRIQFSIIGGNIFPPSRYSDSVVPPI 404
           AIVLDVGSGSVCHPVVLLPTEVE+Q + +    E+   F+           Y    +P  
Sbjct: 314 AIVLDVGSGSVCHPVVLLPTEVEIQSLGLADAGEKDAIFN-----------YRRKYLP-- 373

Query: 405 LACLVAYHTPPRFPVDVIESIREGASLLFKGLGLRDFARIDGWFLPNCCHESSYSTGNFG 464
               VAYHTPPRFP+DVIE+IREGASLLFKGLGL DFARIDGW+LPN  HESS S G FG
Sbjct: 374 -TQQVAYHTPPRFPIDVIETIREGASLLFKGLGLCDFARIDGWYLPNFSHESSCSMGKFG 433

Query: 465 RTESGTVIYTDINLISGMEQTSFLFQQASKVGFSHSNVLRSIIYHACLRYPSLGSLDHMF 524
           RT+SGTV+YTDINLISGMEQTSFLFQQASKVGFSHSN+LRSIIYHACLRYPSLGSLD M 
Sbjct: 434 RTKSGTVVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYPSLGSLDCMS 493

Query: 525 GNWPRRSNSSQRSQAYSSRESLRKVFVIFGGDTSERQVSLMSGTNVWLNLQVFDELEVTP 584
           GN PRRS SSQRSQAYSS ES+RKVFVIFGGDTSERQVSLMSGTNVWLNLQ FDELEVTP
Sbjct: 494 GNTPRRSISSQRSQAYSSHESIRKVFVIFGGDTSERQVSLMSGTNVWLNLQAFDELEVTP 553

Query: 585 CLLAPTNEQTSSVDLDKNEVDVSSRTVWSLPYSLVLRHTTEEVLAACIEAIEPTRAALTS 644
           CLLAP+ EQTS VDLDKNE +++ +TVWSLPYSLVLRHTTEEVLAACIEAIEPTRAALTS
Sbjct: 554 CLLAPSIEQTSGVDLDKNEANLTFKTVWSLPYSLVLRHTTEEVLAACIEAIEPTRAALTS 613

Query: 645 RMRGLVVADLKEGLKKHSWFAGFDIKDELPVRFSLEQWIKQAKEVDATVFIAVHGGIGED 704
            +R LVV DL+EGLKKHSWF+GFDIKDELPVRFSLEQWIK+AKEVDATVFIAVHGGIGED
Sbjct: 614 HLRELVVTDLEEGLKKHSWFSGFDIKDELPVRFSLEQWIKKAKEVDATVFIAVHGGIGED 673

Query: 705 GSLQSLLEAKGVPYTGPGVVASSICMDKVATSLALNHLSDMGVLTIKKDVRRKDDLLDIP 764
           G+LQSLLEA+GVPYTGPGV AS+ICMDKV+TSLALNHLS+MGVLTIKKD RRK+ LL IP
Sbjct: 674 GTLQSLLEAQGVPYTGPGVAASNICMDKVSTSLALNHLSEMGVLTIKKDERRKEYLLHIP 733

Query: 765 IINVWHDLTCKLQCQSLCVKPARDGCSTGVARLCCADDLAVYVKALKDCLARIPSNSFSK 824
           I+NVWHDLTCKLQCQSLCVKPARDGCSTGVARLCCADDLAVYVKAL++CL RIPSNS SK
Sbjct: 734 ILNVWHDLTCKLQCQSLCVKPARDGCSTGVARLCCADDLAVYVKALENCLVRIPSNSLSK 793

Query: 825 AHGMIEMPKPPPELLICEPFIETDEIIVSSKSTNETEERLLWKGQSRWVEITVGVVGTRG 884
           AHGMIEMPKPPPELLI EPFIETDEIIVSSKST+E+ ERLLWKGQSRWVEITVGVVGTRG
Sbjct: 794 AHGMIEMPKPPPELLIFEPFIETDEIIVSSKSTDES-ERLLWKGQSRWVEITVGVVGTRG 853

Query: 885 SMHSLSPSVTVKEHGDILSLEEKFQGGTGINLTPPPLSIISNEALEKCKQHIELISNTLQ 944
           SMHSLSPSVTVKE GDILSLEEKFQGGTGINLTPPPLSIISNE+LEKCKQHIELI+N LQ
Sbjct: 854 SMHSLSPSVTVKESGDILSLEEKFQGGTGINLTPPPLSIISNESLEKCKQHIELIANALQ 913

Query: 945 LEGFSRIDAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQALAETPPVYPHQFFRRLLDLAS 990
           LEGFSRIDAFV+VDSG+VLVIEVNTVPGMTPSTVLIHQALAETPPVYPHQFFRRLLDLA 
Sbjct: 914 LEGFSRIDAFVDVDSGKVLVIEVNTVPGMTPSTVLIHQALAETPPVYPHQFFRRLLDLAL 960

BLAST of Sgr014428 vs. ExPASy Swiss-Prot
Match: Q0SNW3 (D-alanine--D-alanine ligase OS=Borrelia afzelii (strain PKo) OX=390236 GN=ddl PE=3 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 1.8e-21
Identity = 105/389 (26.99%), Postives = 169/389 (43.44%), Query Frame = 0

Query: 113 SAERGISLNSARSV-LDHIQGDDLQVSCYYIDSELNAYAISSAQVYSNTPADFD-FKLKS 172
           S E  IS  SA SV L  +  +   +   YID     + +  +      P D D   + S
Sbjct: 13  SFEHEISCKSAYSVYLALLDLNKYNIYSAYIDKCTGIWYLLDSVSDPPKPIDTDVLPIVS 72

Query: 173 LAHGFRSLTDFAEHLSASVDIVFPVIHGRFGEDGGIQELLERHNIPFVGTGSTECSRAFD 232
           L  GF     F+ + +  +D+VFPV+HGR GEDG IQ +L+  +IP VG G    + + +
Sbjct: 73  LLPGFGI---FSNNKNLEIDVVFPVVHGRTGEDGAIQGVLKVMDIPCVGAGIIGSAISSN 132

Query: 233 KYNASLELDRLGFITVP--NFLVQAGSVNESELSQWFLRNQLDLSSGKVVVKPVRAGSSI 292
           KY   L L       VP   F     S+++ E+     RN  ++    V+VKP   GSSI
Sbjct: 133 KYFCKLLLKSFNIPIVPFIGFRQYDYSLDKEEIK----RNVKEVLGYPVIVKPAVLGSSI 192

Query: 293 GVTVAYGVVDSLKKANEIISKEIDDKVLVEVFLEGGSEFTAIVLDVGSGSVCHPVVLLPT 352
           G+ VAY          E +  + D  +++E F+E      +I   +G+  +    +  P 
Sbjct: 193 GINVAYSENQIESCIEEAL--KYDLTIVIEKFIEAREIECSI---IGNEKM---KIFSPG 252

Query: 353 EVELQFMVRLMWERRIQFSIIGGNIFPPSRYSDSVVPPILACLVAYHTPPRFPVDVIESI 412
           EV +Q  +   ++   ++S+I GN                   + ++ P     + + SI
Sbjct: 253 EVVVQDFI--FYDYDAKYSVIPGN------------------SIIFNIPAHLETNQLLSI 312

Query: 413 REGASLLFKGLGLRDFARIDGWFLPNCCHESSYSTGNFGRTESGTVIYTDINLISGMEQT 472
           +E A L++K L LR  AR+D                 F   +SGT+   +IN I G    
Sbjct: 313 KEYAFLVYKNLELRGMARVD----------------FFVEKKSGTIYLNEINTIPGFTDI 350

Query: 473 SFLFQQASKVGFSHSNVLRSIIYHACLRY 498
           S   +  S  G    +++ ++I +A   Y
Sbjct: 373 SMFSKMCSHDGLQFKDLIDNLIDYAFQSY 350

BLAST of Sgr014428 vs. ExPASy Swiss-Prot
Match: O51218 (D-alanine--D-alanine ligase OS=Borrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680) OX=224326 GN=ddl PE=3 SV=2)

HSP 1 Score: 101.7 bits (252), Expect = 5.8e-20
Identity = 103/391 (26.34%), Postives = 170/391 (43.48%), Query Frame = 0

Query: 113 SAERGISLNSARSV-LDHIQGDDLQVSCYYIDSELNAYAISSAQVYSNTPADFD-FKLKS 172
           S E  IS  SA S+ L  +  +   +   YID     + +  +      P + D   + S
Sbjct: 13  SFEHEISCKSAYSIYLALLDLNKYNIYPVYIDKCTGVWYLLDSVSDPPKPINTDVLPIVS 72

Query: 173 LAHGFRSLTDFAEHLSASVDIVFPVIHGRFGEDGGIQELLERHNIPFVGTGSTECSRAFD 232
           L  GF     F+ + +  +D+VFPV+HGR GEDG IQ +L+  +IP VG G    + + +
Sbjct: 73  LLPGFGI---FSNNKNLEIDVVFPVVHGRTGEDGAIQGVLKVMDIPCVGAGIIGSAISSN 132

Query: 233 KYNASLELDRLGFITVP--NFLVQAGSVNESELSQWFLRNQLDLSSGKVVVKPVRAGSSI 292
           KY   L L       VP   F      +++ E+     RN  ++    V+VKP   GSSI
Sbjct: 133 KYFCKLLLKSFDIPLVPFIGFRQHDYFLDKEEIK----RNVKEVLGYPVIVKPAVLGSSI 192

Query: 293 GVTVAY--GVVDSLKKANEIISKEIDDKVLVEVFLEGGSEFTAIVLDVGSGSVCHPVVLL 352
           G+ VAY    ++S  K     + + D  +++E F+E      +I   +G+  +    +  
Sbjct: 193 GINVAYSENQIESFIKE----ALKYDLTIVIEKFIEAREIECSI---IGNEKM---KIFS 252

Query: 353 PTEVELQFMVRLMWERRIQFSIIGGNIFPPSRYSDSVVPPILACLVAYHTPPRFPVDVIE 412
           P EV +Q  +   ++   ++S+I GN                   + ++ P     + + 
Sbjct: 253 PGEVVVQDFI--FYDYDAKYSVIPGN------------------SIIFNIPAHLETNQLL 312

Query: 413 SIREGASLLFKGLGLRDFARIDGWFLPNCCHESSYSTGNFGRTESGTVIYTDINLISGME 472
           SI+E A L +K L LR  AR+D                 F   +SGT+   +IN I G  
Sbjct: 313 SIKEYAFLTYKNLELRGMARVD----------------FFVEKKSGTIYLNEINTIPGFT 350

Query: 473 QTSFLFQQASKVGFSHSNVLRSIIYHACLRY 498
             S   +  S  G    +++ ++I +A   Y
Sbjct: 373 DISMFAKMCSNDGLQFKDLVDNLIDYAFQSY 350

BLAST of Sgr014428 vs. ExPASy Swiss-Prot
Match: B7J1D2 (D-alanine--D-alanine ligase OS=Borrelia burgdorferi (strain ZS7) OX=445985 GN=ddl PE=3 SV=1)

HSP 1 Score: 100.5 bits (249), Expect = 1.3e-19
Identity = 102/391 (26.09%), Postives = 170/391 (43.48%), Query Frame = 0

Query: 113 SAERGISLNSARSV-LDHIQGDDLQVSCYYIDSELNAYAISSAQVYSNTPADFD-FKLKS 172
           S E  IS  SA S+ L  +  +   +   YID     + +  +      P + D   + S
Sbjct: 13  SFEHEISCKSAYSIYLALLDLNKYNIYPVYIDKCTGVWYLLDSVSDPPKPINTDVLPIVS 72

Query: 173 LAHGFRSLTDFAEHLSASVDIVFPVIHGRFGEDGGIQELLERHNIPFVGTGSTECSRAFD 232
           L  GF     F+ + +  +D+VFPV+HGR GEDG IQ +L+  +IP VG G    + + +
Sbjct: 73  LLPGFGI---FSNNKNLEIDVVFPVVHGRTGEDGAIQGVLKVMDIPCVGAGIIGSAISSN 132

Query: 233 KYNASLELDRLGFITVP--NFLVQAGSVNESELSQWFLRNQLDLSSGKVVVKPVRAGSSI 292
           KY   L L       VP   F      +++ E+     +N  ++    V+VKP   GSSI
Sbjct: 133 KYFCKLLLKSFDIPLVPFIGFRQHDYFLDKEEIK----KNVKEVLGYPVIVKPAVLGSSI 192

Query: 293 GVTVAY--GVVDSLKKANEIISKEIDDKVLVEVFLEGGSEFTAIVLDVGSGSVCHPVVLL 352
           G+ VAY    ++S  K     + + D  +++E F+E      +I   +G+  +    +  
Sbjct: 193 GINVAYSENQIESFIKE----ALKYDLTIVIEKFIEAREIECSI---IGNEKM---KIFS 252

Query: 353 PTEVELQFMVRLMWERRIQFSIIGGNIFPPSRYSDSVVPPILACLVAYHTPPRFPVDVIE 412
           P EV +Q  +   ++   ++S+I GN                   + ++ P     + + 
Sbjct: 253 PGEVVVQDFI--FYDYDAKYSVIPGN------------------SIIFNIPAHLETNQLL 312

Query: 413 SIREGASLLFKGLGLRDFARIDGWFLPNCCHESSYSTGNFGRTESGTVIYTDINLISGME 472
           SI+E A L +K L LR  AR+D                 F   +SGT+   +IN I G  
Sbjct: 313 SIKEYAFLAYKNLELRGMARVD----------------FFVEKKSGTIYLNEINTIPGFT 350

Query: 473 QTSFLFQQASKVGFSHSNVLRSIIYHACLRY 498
             S   +  S  G    +++ ++I +A   Y
Sbjct: 373 DISMFAKMCSNDGLQFKDLVDNLIDYAFQSY 350

BLAST of Sgr014428 vs. ExPASy Swiss-Prot
Match: Q662G4 (D-alanine--D-alanine ligase OS=Borrelia bavariensis (strain ATCC BAA-2496 / DSM 23469 / PBi) OX=290434 GN=ddl PE=3 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 3.8e-19
Identity = 98/388 (25.26%), Postives = 166/388 (42.78%), Query Frame = 0

Query: 113 SAERGISLNSARSV-LDHIQGDDLQVSCYYIDSELNAYAISSAQVYSNTPADFDFKLKSL 172
           S E  IS  SA S+ L  +  +   +   YID     + +  +   S+ P   D  +  +
Sbjct: 13  SFEHEISCKSAYSIYLALLDLNKYNIYPVYIDKCTGIWYLLDS--VSDPPKLIDIDVLPI 72

Query: 173 AHGFRSLTDFAEHLSASVDIVFPVIHGRFGEDGGIQELLERHNIPFVGTGSTECSRAFDK 232
                 +  F+ + +  +D+VFPV+HGR GEDG IQ +L+  +IP +G G    + + +K
Sbjct: 73  VSLLPGIGIFSNNKNLDIDVVFPVVHGRTGEDGAIQGVLKVMDIPCIGAGIIGSAISSNK 132

Query: 233 YNASLELDRLGFITVP--NFLVQAGSVNESELSQWFLRNQLDLSSGKVVVKPVRAGSSIG 292
           Y   L L       VP   F      +++ E+     RN  +     V+VKP   GSSIG
Sbjct: 133 YFCKLLLKSFNIPLVPFIGFRQYDYFLDKEEVK----RNVREALGYPVIVKPAVLGSSIG 192

Query: 293 VTVAYGVVDSLKKANEIISKEIDDKVLVEVFLEGGSEFTAIVLDVGSGSVCHPVVLLPTE 352
           + VAY          E +  + D  +L+E F+E      +I   +G+  +    +  P E
Sbjct: 193 INVAYSENQIEFFIEEAL--KYDLTILIEKFIEAREIECSI---IGNEKI---KIFSPGE 252

Query: 353 VELQFMVRLMWERRIQFSIIGGNIFPPSRYSDSVVPPILACLVAYHTPPRFPVDVIESIR 412
           V +Q  +   ++   ++S+I GN                   + ++ P +   + + SI+
Sbjct: 253 VIVQDFI--FYDYDAKYSVIPGN------------------SIIFNIPAQLETNQLLSIK 312

Query: 413 EGASLLFKGLGLRDFARIDGWFLPNCCHESSYSTGNFGRTESGTVIYTDINLISGMEQTS 472
           E A L++K L LR  AR+D                 F   +SG +   +IN I G    S
Sbjct: 313 EYAFLVYKNLELRGMARVD----------------FFVEKKSGKIYLNEINTIPGFTDIS 350

Query: 473 FLFQQASKVGFSHSNVLRSIIYHACLRY 498
              +  S  G    +++ ++I +A   Y
Sbjct: 373 MFAKMCSHDGLQFKDLVDNLINYAFQSY 350

BLAST of Sgr014428 vs. ExPASy Swiss-Prot
Match: B3EDF4 (D-alanine--D-alanine ligase OS=Chlorobium limicola (strain DSM 245 / NBRC 103803 / 6330) OX=290315 GN=ddl PE=3 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 2.1e-17
Identity = 85/314 (27.07%), Postives = 127/314 (40.45%), Query Frame = 0

Query: 676 VFIAVHGGIGEDGSLQSLLEAKGVPYTGPGVVASSICMDKVATSLALNHLSDMGVLTIKK 735
           VFIA+HG  GEDG +Q LL+  G+PYTG GV AS+I MDK  T L     +D GV T   
Sbjct: 103 VFIALHGSYGEDGRVQGLLDTMGIPYTGCGVSASAIAMDKALTKLC---AADAGVATAPS 162

Query: 736 DVRRKDDLLDIPIINVWHDLTCKLQCQSLCVKPARDGCSTGVARLCCADDLAVYVKALKD 795
                D  L  P     H+L        L VKPA  G S G+++                
Sbjct: 163 ITLDADSYLADP--EPVHELVDSTFGYPLFVKPASLGSSVGISK---------------- 222

Query: 796 CLARIPSNSFSKAHGMIEMPKPPPELLICEPFIETDEIIVSSKSTNETEERLLWKGQSRW 855
                           + +P   PE L               K     + ++L +     
Sbjct: 223 ----------------VHLPAALPEAL---------------KVACSYDRKILVEAAVSG 282

Query: 856 VEITVGVVGTRGSMHSLSPSVTVKEHGDILSLEEKFQGGTGINLTPPPLSIISNEALEKC 915
            EI V V+G    + S+     V+   D    ++K+   T     P  L    ++ L+  
Sbjct: 283 KEIEVAVLGNDRPVASVPGE--VEPGSDFYDFQDKYIHNTAKTFIPARL---PDKLLDSV 342

Query: 916 KQHIELISNTLQLEGFSRIDAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQALAETPPVYP 975
           +     +   L   G SR+D FVN ++G +++ E+NT+PG T   + ++  L E   +  
Sbjct: 343 RCSAITVYKALGCRGMSRVDFFVNEENGSIVLNEINTIPGFT--DISMYPKLFEASGIPF 357

Query: 976 HQFFRRLLDLASER 990
                +LL  A E+
Sbjct: 403 TALIDKLLHYAQEK 357

BLAST of Sgr014428 vs. ExPASy TrEMBL
Match: A0A6J1DW40 (uncharacterized protein LOC111024031 OS=Momordica charantia OX=3673 GN=LOC111024031 PE=4 SV=1)

HSP 1 Score: 1614.4 bits (4179), Expect = 0.0e+00
Identity = 829/956 (86.72%), Postives = 867/956 (90.69%), Query Frame = 0

Query: 44  LSKAVKPRASSFARTLSSSVLRLSLDLNHHTRRLRFPQIRLPRSAAKVVAKDGVAEMAVT 103
           LSK VKPRASSFA TLSSS LR S+D NHHT RLRFP+IR PR+AAKVVAK+GVAEMAVT
Sbjct: 13  LSKTVKPRASSFALTLSSSFLRSSVDSNHHTSRLRFPEIRFPRAAAKVVAKEGVAEMAVT 72

Query: 104 ELEREKQ----------SPSAERGISLNSARSVLDHIQGDDLQVSCYYIDSELNAYAISS 163
           ELERE +           PSAERGISLNSARSVLDHIQGDDL VSCYYID ELNAYAISS
Sbjct: 73  ELERENRGVLRVGLICGGPSAERGISLNSARSVLDHIQGDDLLVSCYYIDPELNAYAISS 132

Query: 164 AQVYSNTPADFDFKLKSLAHGFRSLTDFAEHLSASVDIVFPVIHGRFGEDGGIQELLERH 223
           AQVYSNTPADFDFKLKSLAHGF+SLTDFAEHLSASVDIVFPVIHGRFGEDGGIQELLERH
Sbjct: 133 AQVYSNTPADFDFKLKSLAHGFQSLTDFAEHLSASVDIVFPVIHGRFGEDGGIQELLERH 192

Query: 224 NIPFVGTGSTECSRAFDKYNASLELDRLGFITVPNFLVQAGSVNESELSQWFLRNQLDLS 283
           NIPFVGTGSTECS AFDKYNASLELD+LGFI VPNFL+QAGSV+ESELS+WFLRNQ+D S
Sbjct: 193 NIPFVGTGSTECSHAFDKYNASLELDKLGFIKVPNFLIQAGSVSESELSKWFLRNQIDPS 252

Query: 284 SGKVVVKPVRAGSSIGVTVAYGVVDSLKKANEIISKEIDDKVLVEVFLEGGSEFTAIVLD 343
            GKVVVKPVRAGSSIGVTVAYGV DSLKKA EIIS+EIDDKVLVEVFLEGGSEFTAIVLD
Sbjct: 253 FGKVVVKPVRAGSSIGVTVAYGVADSLKKAEEIISQEIDDKVLVEVFLEGGSEFTAIVLD 312

Query: 344 VGSGSVCHPVVLLPTEVELQFMVRLMWERRIQFSIIGGNIFPPSRYSDSVVPPILACLVA 403
           VGSGSVCHPVVLLPTEVELQF     W    +   I         Y    +P      V 
Sbjct: 313 VGSGSVCHPVVLLPTEVELQFH---GWADAGEKDAI-------FNYRRKYLP---TQQVT 372

Query: 404 YHTPPRFPVDVIESIREGASLLFKGLGLRDFARIDGWFLPNCCHESSYSTGNFGRTESGT 463
           YHTPPRF VDVIESIR+GASLLFKGLGLRDFARIDGWFLPN   ESS S G FGRTESGT
Sbjct: 373 YHTPPRFAVDVIESIRKGASLLFKGLGLRDFARIDGWFLPNSSRESSCSVGKFGRTESGT 432

Query: 464 VIYTDINLISGMEQTSFLFQQASKVGFSHSNVLRSIIYHACLRYPSLGSLDHMFGNWPRR 523
           ++YTDINLISGMEQTSFLFQQASKVGFSHSN+LRSIIYHACLRYP LGSLD M GN  RR
Sbjct: 433 IVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYPDLGSLDCMSGNLLRR 492

Query: 524 SNSSQRSQAYSSRESLRKVFVIFGGDTSERQVSLMSGTNVWLNLQVFDELEVTPCLLAPT 583
           SNS+QRSQAYSS ES+RKVFVIFGGDTSERQVSLMSGTNVWLNLQVFDELEVTPCLLAP+
Sbjct: 493 SNSAQRSQAYSSHESIRKVFVIFGGDTSERQVSLMSGTNVWLNLQVFDELEVTPCLLAPS 552

Query: 584 NEQTSSVDLDKNEVDVSSRTVWSLPYSLVLRHTTEEVLAACIEAIEPTRAALTSRMRGLV 643
           NEQTSS+DLDKN+VD +SRTVWSLPYSLVLRHTTEEVLAACIEA+EPTRAALTSR+R LV
Sbjct: 553 NEQTSSLDLDKNQVDETSRTVWSLPYSLVLRHTTEEVLAACIEAMEPTRAALTSRLRELV 612

Query: 644 VADLKEGLKKHSWFAGFDIKDELPVRFSLEQWIKQAKEVDATVFIAVHGGIGEDGSLQSL 703
           + DLKEGLK H WFAGFDIKDELPVR+SLEQWIKQAKE DATVFIAVHGGIGEDG+LQSL
Sbjct: 613 MTDLKEGLKNHRWFAGFDIKDELPVRYSLEQWIKQAKEADATVFIAVHGGIGEDGTLQSL 672

Query: 704 LEAKGVPYTGPGVVASSICMDKVATSLALNHLSDMGVLTIKKDVRRKDDLLDIPIINVWH 763
           LEAKGVPYTGPG  AS+ICMDKVATSLALNHLSDMGVLTIKKDVRRKDDLL IPI+NVWH
Sbjct: 673 LEAKGVPYTGPGAAASNICMDKVATSLALNHLSDMGVLTIKKDVRRKDDLLQIPILNVWH 732

Query: 764 DLTCKLQCQSLCVKPARDGCSTGVARLCCADDLAVYVKALKDCLARIPSNSFSKAHGMIE 823
           DLT KL CQSLCVKPARDGCSTGVARLCCADDLAVYVKAL+DCL RIPSNS SKAHGMIE
Sbjct: 733 DLTRKLHCQSLCVKPARDGCSTGVARLCCADDLAVYVKALEDCLVRIPSNSLSKAHGMIE 792

Query: 824 MPKPPPELLICEPFIETDEIIVSSKSTNETEERLLWKGQSRWVEITVGVVGTRGSMHSLS 883
           MPKPPPELLI EPFIETDEIIVSSKSTNET+E+LLW+GQSRWVEITVGVVGTRGSMHSLS
Sbjct: 793 MPKPPPELLIFEPFIETDEIIVSSKSTNETKEQLLWEGQSRWVEITVGVVGTRGSMHSLS 852

Query: 884 PSVTVKEHGDILSLEEKFQGGTGINLTPPPLSIISNEALEKCKQHIELISNTLQLEGFSR 943
           PSVTVKE GDILSLEEKFQGGTGINLTPPPL II NEALEKCK+HIELI+NTLQLEGFSR
Sbjct: 853 PSVTVKESGDILSLEEKFQGGTGINLTPPPLMIIRNEALEKCKRHIELIANTLQLEGFSR 912

Query: 944 IDAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQALAETPPVYPHQFFRRLLDLASER 990
           IDAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQALAETPPVYPHQFFRRLL+LASER
Sbjct: 913 IDAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQALAETPPVYPHQFFRRLLNLASER 955

BLAST of Sgr014428 vs. ExPASy TrEMBL
Match: A0A6J1F3B4 (uncharacterized protein LOC111441803 OS=Cucurbita moschata OX=3662 GN=LOC111441803 PE=4 SV=1)

HSP 1 Score: 1590.9 bits (4118), Expect = 0.0e+00
Identity = 821/960 (85.52%), Postives = 864/960 (90.00%), Query Frame = 0

Query: 44  LSKAVKPRASSFARTLSSSVLRLSLDLNHHTRRLRFPQIRLPRSAAKVVAKDGVAEMAVT 103
           L K +K RAS+F  TLSSS LRLSLDL++H RRL+ P+IR+ R+AAK+V KDGVAEMA T
Sbjct: 13  LCKDIKLRASNFTPTLSSSFLRLSLDLHNHARRLQSPKIRVSRAAAKMVVKDGVAEMATT 72

Query: 104 ELEREKQS--------------PSAERGISLNSARSVLDHIQGDDLQVSCYYIDSELNAY 163
           E+EREK S              PSAERGISLNSARSVLDHIQGDDLQVSCYYID ELNAY
Sbjct: 73  EIEREKHSVKCEALRVGLICGGPSAERGISLNSARSVLDHIQGDDLQVSCYYIDPELNAY 132

Query: 164 AISSAQVYSNTPADFDFKLKSLAHGFRSLTDFAEHLSASVDIVFPVIHGRFGEDGGIQEL 223
           AISSAQVYSNTPADFDFKLKSLA GFRSLTDFAEHLS SVDIVFPVIHGRFGEDGGIQEL
Sbjct: 133 AISSAQVYSNTPADFDFKLKSLARGFRSLTDFAEHLSTSVDIVFPVIHGRFGEDGGIQEL 192

Query: 224 LERHNIPFVGTGSTECSRAFDKYNASLELDRLGFITVPNFLVQAGSVNESELSQWFLRNQ 283
           LE HNIPFVGTGSTEC+ AFDKYNA+LELDRLGFITVPNFLVQAGSV+ES+LS+WFLRNQ
Sbjct: 193 LETHNIPFVGTGSTECAHAFDKYNAALELDRLGFITVPNFLVQAGSVSESDLSKWFLRNQ 252

Query: 284 LDLSSGKVVVKPVRAGSSIGVTVAYGVVDSLKKANEIISKEIDDKVLVEVFLEGGSEFTA 343
           LDLSSGKVVVKPVRAGSSIGV+VAYGVVDSLKKANEIISKEIDDKVLVEVFLE GSEFTA
Sbjct: 253 LDLSSGKVVVKPVRAGSSIGVSVAYGVVDSLKKANEIISKEIDDKVLVEVFLERGSEFTA 312

Query: 344 IVLDVGSGSVCHPVVLLPTEVELQFMVRLMWERRIQFSIIGGNIFPPSRYSDSVVPPILA 403
           IVLDVGSGSVCHPVVLLPTEVEL+      W    +   I         Y    +P    
Sbjct: 313 IVLDVGSGSVCHPVVLLPTEVELESHA---WADAGEKDAI-------FNYRRKYLP---T 372

Query: 404 CLVAYHTPPRFPVDVIESIREGASLLFKGLGLRDFARIDGWFLPNCCHESSYSTGNFGRT 463
             VAYHTPPRFP+DVIESIREGASLLFKGLGL DFARIDGWFLPN  H+SS STG FGRT
Sbjct: 373 QQVAYHTPPRFPIDVIESIREGASLLFKGLGLCDFARIDGWFLPNSSHDSSSSTGKFGRT 432

Query: 464 ESGTVIYTDINLISGMEQTSFLFQQASKVGFSHSNVLRSIIYHACLRYPSLGSLDHMFGN 523
            SGTV+YTDINLISGMEQTSFLFQQASKVGFSHSN+LRSIIYHACLRYPSLGSLD M GN
Sbjct: 433 ASGTVVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYPSLGSLDCMSGN 492

Query: 524 WPRRSNSSQRSQAYSSRESLRKVFVIFGGDTSERQVSLMSGTNVWLNLQVFDELEVTPCL 583
            PRRS SSQR+QAYSS ES +KVFVI GGDTSERQVSLMSGTNVWLNLQ FD+LEVTPCL
Sbjct: 493 MPRRSISSQRTQAYSSTESNQKVFVILGGDTSERQVSLMSGTNVWLNLQAFDDLEVTPCL 552

Query: 584 LAPTNEQTSSVDLDKNEVDVSSRTVWSLPYSLVLRHTTEEVLAACIEAIEPTRAALTSRM 643
           LAP+ EQTSSVDLDKNE D++SRTVWSLPYSLVLRHTTEEVLAACIEA EPTRAALTS +
Sbjct: 553 LAPSIEQTSSVDLDKNEADMTSRTVWSLPYSLVLRHTTEEVLAACIEAFEPTRAALTSHL 612

Query: 644 RGLVVADLKEGLKKHSWFAGFDIKDELPVRFSLEQWIKQAKEVDATVFIAVHGGIGEDGS 703
           R LVV DL+EGLKKHSWFAGFDIKDELPV+FSLEQWIK+AKEVDATVFIAVHGGIGE+G 
Sbjct: 613 RELVVTDLEEGLKKHSWFAGFDIKDELPVKFSLEQWIKKAKEVDATVFIAVHGGIGENGM 672

Query: 704 LQSLLEAKGVPYTGPGVVASSICMDKVATSLALNHLSDMGVLTIKKDVRRKDDLLDIPII 763
           LQSLLEAKGVPYTGPGV AS+ICMDKVATSLALNHLSDMGVLTIKKD RRKD+LL IP +
Sbjct: 673 LQSLLEAKGVPYTGPGVAASNICMDKVATSLALNHLSDMGVLTIKKDARRKDELLHIPTL 732

Query: 764 NVWHDLTCKLQCQSLCVKPARDGCSTGVARLCCADDLAVYVKALKDCLARIPSNSFSKAH 823
           N+WHDLTCKLQCQSLCVKPARDGCSTGVARLCCADDLAVYVKAL+DCL RIPSNS S+AH
Sbjct: 733 NLWHDLTCKLQCQSLCVKPARDGCSTGVARLCCADDLAVYVKALEDCLVRIPSNSLSRAH 792

Query: 824 GMIEMPKPPPELLICEPFIETDEIIVSSKSTNETEERLLWKGQSRWVEITVGVVGTRGSM 883
           GMIEMPKPPPELLI EPFIETDEIIVSSK TNE+ ERLLWKGQSRWVEITVGVVGTRGSM
Sbjct: 793 GMIEMPKPPPELLIFEPFIETDEIIVSSKYTNES-ERLLWKGQSRWVEITVGVVGTRGSM 852

Query: 884 HSLSPSVTVKEHGDILSLEEKFQGGTGINLTPPPLSIISNEALEKCKQHIELISNTLQLE 943
           HSLSPSVTVKE GDILSLEEKFQGGTGINLTPPPLSIISNE+LEKCKQHIELI+NTLQLE
Sbjct: 853 HSLSPSVTVKESGDILSLEEKFQGGTGINLTPPPLSIISNESLEKCKQHIELIANTLQLE 912

Query: 944 GFSRIDAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQALAETPPVYPHQFFRRLLDLASER 990
           GFSRIDAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQALAETPPVYPHQFFRRLLDLA ER
Sbjct: 913 GFSRIDAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQALAETPPVYPHQFFRRLLDLAWER 958

BLAST of Sgr014428 vs. ExPASy TrEMBL
Match: A0A6J1IXB9 (uncharacterized protein LOC111481502 OS=Cucurbita maxima OX=3661 GN=LOC111481502 PE=4 SV=1)

HSP 1 Score: 1588.5 bits (4112), Expect = 0.0e+00
Identity = 819/960 (85.31%), Postives = 863/960 (89.90%), Query Frame = 0

Query: 44  LSKAVKPRASSFARTLSSSVLRLSLDLNHHTRRLRFPQIRLPRSAAKVVAKDGVAEMAVT 103
           L K ++PRAS+F  TLSSS LRLSLDL++H RRL+ P+IR+ R+AAK+V KDGVAEMA T
Sbjct: 13  LCKDIEPRASNFTPTLSSSFLRLSLDLHNHARRLQSPKIRVSRAAAKMVVKDGVAEMAAT 72

Query: 104 ELEREKQS--------------PSAERGISLNSARSVLDHIQGDDLQVSCYYIDSELNAY 163
           E+EREK S              PSAERGISLNSARSVLDHIQGDDLQVSCYYID ELNAY
Sbjct: 73  EIEREKHSVKCEALRVGLICGGPSAERGISLNSARSVLDHIQGDDLQVSCYYIDPELNAY 132

Query: 164 AISSAQVYSNTPADFDFKLKSLAHGFRSLTDFAEHLSASVDIVFPVIHGRFGEDGGIQEL 223
           AISSAQVYSNTPADFDFKLKSLA GFRSLTDFAEHLS SVDIVFPVIHGRFGEDGGIQEL
Sbjct: 133 AISSAQVYSNTPADFDFKLKSLARGFRSLTDFAEHLSTSVDIVFPVIHGRFGEDGGIQEL 192

Query: 224 LERHNIPFVGTGSTECSRAFDKYNASLELDRLGFITVPNFLVQAGSVNESELSQWFLRNQ 283
           LE HNIPFVGTGSTEC+ AFDKYNASLELDRLGFITVPNFLVQAGSV+ES+LS+WFLRNQ
Sbjct: 193 LETHNIPFVGTGSTECAHAFDKYNASLELDRLGFITVPNFLVQAGSVSESDLSKWFLRNQ 252

Query: 284 LDLSSGKVVVKPVRAGSSIGVTVAYGVVDSLKKANEIISKEIDDKVLVEVFLEGGSEFTA 343
           LDLSSGKVVVKP RAGSSIGV+VAYGVVDSLKKANEIISKEIDDKVLVEVFLE GSEFTA
Sbjct: 253 LDLSSGKVVVKPARAGSSIGVSVAYGVVDSLKKANEIISKEIDDKVLVEVFLERGSEFTA 312

Query: 344 IVLDVGSGSVCHPVVLLPTEVELQFMVRLMWERRIQFSIIGGNIFPPSRYSDSVVPPILA 403
           IVLDVGSGSVCHPVVLLPTEVELQ      W    +   I         Y    +P    
Sbjct: 313 IVLDVGSGSVCHPVVLLPTEVELQ---SHGWADAGEKDAI-------FNYRRKYLP---T 372

Query: 404 CLVAYHTPPRFPVDVIESIREGASLLFKGLGLRDFARIDGWFLPNCCHESSYSTGNFGRT 463
             VAYHTPPRFP+DVIESIREGASLLFKGLGL DFARIDGWFLPN  H+SS STG FGRT
Sbjct: 373 QQVAYHTPPRFPIDVIESIREGASLLFKGLGLCDFARIDGWFLPNSSHDSSSSTGKFGRT 432

Query: 464 ESGTVIYTDINLISGMEQTSFLFQQASKVGFSHSNVLRSIIYHACLRYPSLGSLDHMFGN 523
            SGTV+YTDINLISGMEQTSFLFQQASKVGFSHSN+LRSIIYHACLRYPSLGSLD M GN
Sbjct: 433 ASGTVVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYPSLGSLDCMSGN 492

Query: 524 WPRRSNSSQRSQAYSSRESLRKVFVIFGGDTSERQVSLMSGTNVWLNLQVFDELEVTPCL 583
            PRRS SSQRSQAYSS ES +KVF+IFGGDTSERQVSLMSGTNVWLNLQ FD+LEVTPCL
Sbjct: 493 MPRRSISSQRSQAYSSTESNQKVFLIFGGDTSERQVSLMSGTNVWLNLQAFDDLEVTPCL 552

Query: 584 LAPTNEQTSSVDLDKNEVDVSSRTVWSLPYSLVLRHTTEEVLAACIEAIEPTRAALTSRM 643
           LAP+ EQTSSVDLDKNE D++S+TVWSLPYSLVLRHTTEEVLAACIEA EPTRAALTS +
Sbjct: 553 LAPSIEQTSSVDLDKNEADMTSKTVWSLPYSLVLRHTTEEVLAACIEAFEPTRAALTSHL 612

Query: 644 RGLVVADLKEGLKKHSWFAGFDIKDELPVRFSLEQWIKQAKEVDATVFIAVHGGIGEDGS 703
           R LVV DL+EGLKKHSWFAGFDIKDELPV+FSLEQWIK+AKEVDATVFIAVHGGIGE+G 
Sbjct: 613 RELVVTDLEEGLKKHSWFAGFDIKDELPVKFSLEQWIKKAKEVDATVFIAVHGGIGENGM 672

Query: 704 LQSLLEAKGVPYTGPGVVASSICMDKVATSLALNHLSDMGVLTIKKDVRRKDDLLDIPII 763
           LQSLLEAKGVPYTGPGV AS+ICMDKVATSLALNHLSDMGVLTIKKD RRKD+LL+IP +
Sbjct: 673 LQSLLEAKGVPYTGPGVAASNICMDKVATSLALNHLSDMGVLTIKKDARRKDELLNIPTL 732

Query: 764 NVWHDLTCKLQCQSLCVKPARDGCSTGVARLCCADDLAVYVKALKDCLARIPSNSFSKAH 823
           N+WHDLTCKLQCQSLCVKPARDGCSTGVARLCCADDLAVY KAL+ CL RIPSNS S+AH
Sbjct: 733 NLWHDLTCKLQCQSLCVKPARDGCSTGVARLCCADDLAVYAKALEYCLVRIPSNSLSRAH 792

Query: 824 GMIEMPKPPPELLICEPFIETDEIIVSSKSTNETEERLLWKGQSRWVEITVGVVGTRGSM 883
           GMIEMPKPPPELLI EPFIETDEIIVSSK TNE+ ERLLWKGQSRWVEITVGVVGTRGSM
Sbjct: 793 GMIEMPKPPPELLIFEPFIETDEIIVSSKYTNES-ERLLWKGQSRWVEITVGVVGTRGSM 852

Query: 884 HSLSPSVTVKEHGDILSLEEKFQGGTGINLTPPPLSIISNEALEKCKQHIELISNTLQLE 943
           HSLSPSVTVKE GDILSLEEKFQGGTGINLTPPPLSIISNE+LEKCKQHIELI+N LQLE
Sbjct: 853 HSLSPSVTVKESGDILSLEEKFQGGTGINLTPPPLSIISNESLEKCKQHIELIANALQLE 912

Query: 944 GFSRIDAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQALAETPPVYPHQFFRRLLDLASER 990
           GFSRIDAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQALAETPPVYPHQFFRRLLDLA ER
Sbjct: 913 GFSRIDAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQALAETPPVYPHQFFRRLLDLALER 958

BLAST of Sgr014428 vs. ExPASy TrEMBL
Match: A0A1S3B5H4 (uncharacterized protein LOC103486245 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103486245 PE=4 SV=1)

HSP 1 Score: 1560.4 bits (4039), Expect = 0.0e+00
Identity = 813/961 (84.60%), Postives = 858/961 (89.28%), Query Frame = 0

Query: 44  LSKAVKPRASSFARTLSSSVLRLSLDLNHHTRRLRFPQIRLPRSAAKVVA-KDGVAEMAV 103
           LS  +K   S+FA TLSSS LRLSL  N+HT  L  P+ +LPRSAAKV+A KD VAEMAV
Sbjct: 15  LSDVLKLTPSTFAPTLSSSFLRLSLQSNNHTPPLHSPKFQLPRSAAKVLANKDVVAEMAV 74

Query: 104 TELEREKQS--------------PSAERGISLNSARSVLDHIQGDDLQVSCYYIDSELNA 163
           T  EREKQS              PSAERGISLNSARSVLDHIQG DLQVSCYYIDSELNA
Sbjct: 75  TRTEREKQSEKCGALRVGLICGGPSAERGISLNSARSVLDHIQGHDLQVSCYYIDSELNA 134

Query: 164 YAISSAQVYSNTPADFDFKLKSLAHGFRSLTDFAEHLSASVDIVFPVIHGRFGEDGGIQE 223
           YAISSAQVYSNTPADFDFKLKSLA GFRSLTDFAEHLS SVDIVFPVIHGRFGEDGGIQE
Sbjct: 135 YAISSAQVYSNTPADFDFKLKSLARGFRSLTDFAEHLSTSVDIVFPVIHGRFGEDGGIQE 194

Query: 224 LLERHNIPFVGTGSTECSRAFDKYNASLELDRLGFITVPNFLVQAGSVNESELSQWFLRN 283
           LLE HNIPFVGTGSTE SRAFDKY+ASLELDRLGFITVPNFLVQAGSV+E+ELS+WF+ N
Sbjct: 195 LLETHNIPFVGTGSTESSRAFDKYDASLELDRLGFITVPNFLVQAGSVSETELSKWFVSN 254

Query: 284 QLDLSSGKVVVKPVRAGSSIGVTVAYGVVDSLKKANEIISKEIDDKVLVEVFLEGGSEFT 343
           QLDLSSGKVVVKP RAGSSIGV+VAYGVVDSLKKANEIIS+EIDDKVLVEVFLEGGSEFT
Sbjct: 255 QLDLSSGKVVVKPARAGSSIGVSVAYGVVDSLKKANEIISEEIDDKVLVEVFLEGGSEFT 314

Query: 344 AIVLDVGSGSVCHPVVLLPTEVELQFMVRLMWERRIQFSIIGGNIFPPSRYSDSVVPPIL 403
           AIVLDVGSGSVCHPVVLLPTEVE+Q        R +  +     IF    Y    +P   
Sbjct: 315 AIVLDVGSGSVCHPVVLLPTEVEIQ-------SRDVADAGEKDAIF---NYRRKYLP--- 374

Query: 404 ACLVAYHTPPRFPVDVIESIREGASLLFKGLGLRDFARIDGWFLPNCCHESSYSTGNFGR 463
              VAYHTPPRFP+DVIE+IREGASLLFKGLGL DFARIDGW+LP+  HESS STG FG+
Sbjct: 375 TQQVAYHTPPRFPIDVIETIREGASLLFKGLGLCDFARIDGWYLPSFSHESSCSTGKFGK 434

Query: 464 TESGTVIYTDINLISGMEQTSFLFQQASKVGFSHSNVLRSIIYHACLRYPSLGSLDHMFG 523
           TESGTV+YTDINLISGMEQTSFLFQQASKVGFSHSN+LRSIIYHACLRYPSLGSLD + G
Sbjct: 435 TESGTVVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYPSLGSLDCISG 494

Query: 524 NWPRRSNSSQRSQAYSSRESLRKVFVIFGGDTSERQVSLMSGTNVWLNLQVFDELEVTPC 583
           N PRRS SSQ SQAYS+ ES+RKVFVIFGGDTSERQVSLMSGTNVWLNLQ FDELEVTPC
Sbjct: 495 NVPRRSISSQCSQAYSTHESIRKVFVIFGGDTSERQVSLMSGTNVWLNLQAFDELEVTPC 554

Query: 584 LLAPTNEQTSSVDLDKNEVDVSSRTVWSLPYSLVLRHTTEEVLAACIEAIEPTRAALTSR 643
           LLAP+ EQ SSVDLDKNE D++SRTVWSLPYSLVLRHTTEEVLAACIEAIEPTRAALTS 
Sbjct: 555 LLAPSIEQASSVDLDKNEADLTSRTVWSLPYSLVLRHTTEEVLAACIEAIEPTRAALTSH 614

Query: 644 MRGLVVADLKEGLKKHSWFAGFDIKDELPVRFSLEQWIKQAKEVDATVFIAVHGGIGEDG 703
           +R LVV DL+EGLKKHSWFAGFDIKDELPVRFSLEQWI++AKEVDATVFIAVHGGIGEDG
Sbjct: 615 LRELVVNDLEEGLKKHSWFAGFDIKDELPVRFSLEQWIEKAKEVDATVFIAVHGGIGEDG 674

Query: 704 SLQSLLEAKGVPYTGPGVVASSICMDKVATSLALNHLSDMGVLTIKKDVRRKDDLLDIPI 763
           +LQSLLEAKGVPYTGPGV AS+ICMDKV+TSLALNHLSD GVLTIKKDVR+KDDLL  PI
Sbjct: 675 TLQSLLEAKGVPYTGPGVAASNICMDKVSTSLALNHLSDFGVLTIKKDVRKKDDLLRFPI 734

Query: 764 INVWHDLTCKLQCQSLCVKPARDGCSTGVARLCCADDLAVYVKALKDCLARIPSNSFSKA 823
           +NVW DLTCKLQCQSLCVKPARDGCSTGVARLCCADDLAVYVKAL+DCL RIPSNS SKA
Sbjct: 735 LNVWRDLTCKLQCQSLCVKPARDGCSTGVARLCCADDLAVYVKALEDCLVRIPSNSLSKA 794

Query: 824 HGMIEMPKPPPELLICEPFIETDEIIVSSKSTNETEERLLWKGQSRWVEITVGVVGTRGS 883
           HGMIEMPKPPPELLI EPFIETDEIIVSSK T +  ERLLWKGQSRWVEITVGVVGTRGS
Sbjct: 795 HGMIEMPKPPPELLIFEPFIETDEIIVSSK-TIDASERLLWKGQSRWVEITVGVVGTRGS 854

Query: 884 MHSLSPSVTVKEHGDILSLEEKFQGGTGINLTPPPLSIISNEALEKCKQHIELISNTLQL 943
           MHSLSPSVTVKE GDILSLEEKFQGGTGINLTPPPLSIISNE+L KCKQHIELI+N LQL
Sbjct: 855 MHSLSPSVTVKESGDILSLEEKFQGGTGINLTPPPLSIISNESLGKCKQHIELIANALQL 914

Query: 944 EGFSRIDAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQALAETPPVYPHQFFRRLLDLASE 990
           EGFSRIDAFV+VDSG+VLVIEVNTVPGMTPSTVLIHQALAETPPVYPHQFFRRLLDLA E
Sbjct: 915 EGFSRIDAFVDVDSGKVLVIEVNTVPGMTPSTVLIHQALAETPPVYPHQFFRRLLDLALE 961

BLAST of Sgr014428 vs. ExPASy TrEMBL
Match: A0A5A7TLK1 (D-alanine--D-alanine ligase family protein isoform 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold236G005710 PE=4 SV=1)

HSP 1 Score: 1559.3 bits (4036), Expect = 0.0e+00
Identity = 812/961 (84.50%), Postives = 857/961 (89.18%), Query Frame = 0

Query: 44  LSKAVKPRASSFARTLSSSVLRLSLDLNHHTRRLRFPQIRLPRSAAKVVA-KDGVAEMAV 103
           LS  +K   S+FA TLSSS LRLSL  N+HT  L  P+ +LPRSAAKV+A KD VAEMAV
Sbjct: 15  LSDVLKLTPSTFAPTLSSSFLRLSLQSNNHTPPLHSPKFQLPRSAAKVLANKDVVAEMAV 74

Query: 104 TELEREKQS--------------PSAERGISLNSARSVLDHIQGDDLQVSCYYIDSELNA 163
           T  EREKQS              PSAERGISLNSARSVLDHIQG DLQVSCYYIDSELNA
Sbjct: 75  TRTEREKQSEKCGALRVGLICGGPSAERGISLNSARSVLDHIQGHDLQVSCYYIDSELNA 134

Query: 164 YAISSAQVYSNTPADFDFKLKSLAHGFRSLTDFAEHLSASVDIVFPVIHGRFGEDGGIQE 223
           YAISSAQVYSNTPADFDFKLKSLA GFRSLTDFAEHLS SVDIVFPVIHGRFGEDGGIQE
Sbjct: 135 YAISSAQVYSNTPADFDFKLKSLARGFRSLTDFAEHLSTSVDIVFPVIHGRFGEDGGIQE 194

Query: 224 LLERHNIPFVGTGSTECSRAFDKYNASLELDRLGFITVPNFLVQAGSVNESELSQWFLRN 283
           LLE HNIPFVGTGSTE SRAFDKY+ASLELDRLGFITVPNFLVQAGSV+E+ELS+WF+ N
Sbjct: 195 LLETHNIPFVGTGSTESSRAFDKYDASLELDRLGFITVPNFLVQAGSVSETELSKWFVSN 254

Query: 284 QLDLSSGKVVVKPVRAGSSIGVTVAYGVVDSLKKANEIISKEIDDKVLVEVFLEGGSEFT 343
           QLDLSSGKVVVKP RAGSSIGV+VAYGVVDSLKKANEIIS+EIDDKVLVEVFLEGGSEFT
Sbjct: 255 QLDLSSGKVVVKPARAGSSIGVSVAYGVVDSLKKANEIISEEIDDKVLVEVFLEGGSEFT 314

Query: 344 AIVLDVGSGSVCHPVVLLPTEVELQFMVRLMWERRIQFSIIGGNIFPPSRYSDSVVPPIL 403
           AIVLDVGSGSVCHPVVLLPTEVE+Q        R +  +     IF    Y    +P   
Sbjct: 315 AIVLDVGSGSVCHPVVLLPTEVEIQ-------SRDVADAGEKDAIF---NYRRKYLP--- 374

Query: 404 ACLVAYHTPPRFPVDVIESIREGASLLFKGLGLRDFARIDGWFLPNCCHESSYSTGNFGR 463
              VAYHTPPRFP+DVIE+IREGASLLFKGLGL DFARIDGW+LP+  HESS STG FG+
Sbjct: 375 TQQVAYHTPPRFPIDVIETIREGASLLFKGLGLCDFARIDGWYLPSFSHESSCSTGKFGK 434

Query: 464 TESGTVIYTDINLISGMEQTSFLFQQASKVGFSHSNVLRSIIYHACLRYPSLGSLDHMFG 523
           TESGTV+YTDINLISGMEQTSFLFQQASKVGFSHSN+LRSIIYHACLRY SLGSLD + G
Sbjct: 435 TESGTVVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYSSLGSLDCISG 494

Query: 524 NWPRRSNSSQRSQAYSSRESLRKVFVIFGGDTSERQVSLMSGTNVWLNLQVFDELEVTPC 583
           N PRRS SSQRSQAYS+ ES+RKVFVIFGGDTSERQVSLMSGTNVWLNLQ FDELEVTPC
Sbjct: 495 NVPRRSISSQRSQAYSTHESIRKVFVIFGGDTSERQVSLMSGTNVWLNLQAFDELEVTPC 554

Query: 584 LLAPTNEQTSSVDLDKNEVDVSSRTVWSLPYSLVLRHTTEEVLAACIEAIEPTRAALTSR 643
           LLAP+ EQ SSVDLDKNE D++SRTVWSLPYSLVLRHTTEEVLAACIEAIEPTRAALTS 
Sbjct: 555 LLAPSIEQASSVDLDKNEADLTSRTVWSLPYSLVLRHTTEEVLAACIEAIEPTRAALTSH 614

Query: 644 MRGLVVADLKEGLKKHSWFAGFDIKDELPVRFSLEQWIKQAKEVDATVFIAVHGGIGEDG 703
           +R LVV DL+EGLKKHSWFAGFDIKDELPVRFSLEQWI++AKEVDATVFIAVHGGIGEDG
Sbjct: 615 LRELVVTDLEEGLKKHSWFAGFDIKDELPVRFSLEQWIEKAKEVDATVFIAVHGGIGEDG 674

Query: 704 SLQSLLEAKGVPYTGPGVVASSICMDKVATSLALNHLSDMGVLTIKKDVRRKDDLLDIPI 763
           +LQSLLEAKGVPYTGPGV AS+ICMDKV+TSLALNHLSD GVLT KKDVR+KDDLL  PI
Sbjct: 675 TLQSLLEAKGVPYTGPGVAASNICMDKVSTSLALNHLSDFGVLTTKKDVRKKDDLLRFPI 734

Query: 764 INVWHDLTCKLQCQSLCVKPARDGCSTGVARLCCADDLAVYVKALKDCLARIPSNSFSKA 823
           +NVW DLTCKLQCQSLCVKPARDGCSTGVARLCCADDLAVYVKAL+DCL RIPSNS SKA
Sbjct: 735 LNVWRDLTCKLQCQSLCVKPARDGCSTGVARLCCADDLAVYVKALEDCLVRIPSNSLSKA 794

Query: 824 HGMIEMPKPPPELLICEPFIETDEIIVSSKSTNETEERLLWKGQSRWVEITVGVVGTRGS 883
           HGMIEMPKPPPELLI EPFIETDEIIVSSK T +  ERLLWKGQSRWVEITVGVVGTRGS
Sbjct: 795 HGMIEMPKPPPELLIFEPFIETDEIIVSSK-TIDASERLLWKGQSRWVEITVGVVGTRGS 854

Query: 884 MHSLSPSVTVKEHGDILSLEEKFQGGTGINLTPPPLSIISNEALEKCKQHIELISNTLQL 943
           MHSLSPSVTVKE GDILSLEEKFQGGTGINLTPPPLSIISNE+L KCKQHIELI+N LQL
Sbjct: 855 MHSLSPSVTVKESGDILSLEEKFQGGTGINLTPPPLSIISNESLGKCKQHIELIANALQL 914

Query: 944 EGFSRIDAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQALAETPPVYPHQFFRRLLDLASE 990
           EGFSRIDAFV+VDSG+VLVIEVNTVPGMTPSTVLIHQALAETPPVYPHQFFRRLLDLA E
Sbjct: 915 EGFSRIDAFVDVDSGKVLVIEVNTVPGMTPSTVLIHQALAETPPVYPHQFFRRLLDLALE 961

BLAST of Sgr014428 vs. TAIR 10
Match: AT3G08840.2 (D-alanine--D-alanine ligase family )

HSP 1 Score: 1142.1 bits (2953), Expect = 0.0e+00
Identity = 591/885 (66.78%), Postives = 694/885 (78.42%), Query Frame = 0

Query: 112 PSAERGISLNSARSVLDHIQGDDLQVSCYYIDSELNAYAISSAQVYSNTPADFDFKLKSL 171
           PSAERGISLNSARSVLDHIQGD + VSCYYID +L A+AISSAQVYSNTP+DFDFKL+SL
Sbjct: 87  PSAERGISLNSARSVLDHIQGDGINVSCYYIDPDLKAFAISSAQVYSNTPSDFDFKLESL 146

Query: 172 AHGFRSLTDFAEHLSASVDIVFPVIHGRFGEDGGIQELLERHNIPFVGTGSTECSRAFDK 231
           A GF SL++ AEHL ++VDIVFPVIHGRFGEDGGIQELLE HNIPFVGTGS EC RAFDK
Sbjct: 147 AQGFSSLSELAEHLVSAVDIVFPVIHGRFGEDGGIQELLESHNIPFVGTGSRECFRAFDK 206

Query: 232 YNASLELDRLGFITVPNFLVQAGSVNESELSQWFLRNQLDLSSGKVVVKPVRAGSSIGVT 291
           Y ASLEL  LGF+TVPN+LVQ   V++SE++ WF  NQLDL  GKVVVKP +AGSSIGV 
Sbjct: 207 YEASLELKELGFMTVPNYLVQGTGVDKSEIALWFTDNQLDLEMGKVVVKPAKAGSSIGVK 266

Query: 292 VAYGVVDSLKKANEIISKEIDDKVLVEVFLEGGSEFTAIVLDVGSGSVCHPVVLLPTEVE 351
           VA+GV DS+KKA E+I + IDD+V+VEVF+E   EFTAIVLDVGSGSVCHPVVL+PTEV+
Sbjct: 267 VAFGVNDSIKKATELILEGIDDRVVVEVFIEDAYEFTAIVLDVGSGSVCHPVVLMPTEVQ 326

Query: 352 LQFMVRLMWERRIQFSIIGGNIFPPSR-----YSDSVVPPILACLVAYHTPPRFPVDVIE 411
           LQF                  I  P       Y    +P      V YHTPPRFP+ VI+
Sbjct: 327 LQF----------------HGIGDPKENAIFDYRRKYLP---TQQVTYHTPPRFPIHVIK 386

Query: 412 SIREGASLLFKGLGLRDFARIDGWFLPNCCHESSYSTGNFGRTESGTVIYTDINLISGME 471
           SIRE ASL+F+ LGLRDFARIDGW+L    + SS  +   G T+SG +I+TDINLISGME
Sbjct: 387 SIREEASLIFQKLGLRDFARIDGWYLAPNSNLSSPVSETLGGTKSGDIIFTDINLISGME 446

Query: 472 QTSFLFQQASKVGFSHSNVLRSIIYHACLRYPSLGSLDHMFGNWPRRSNSSQRSQAYSSR 531
           QTSFLFQQASKVGFSHSN+LR+I++ AC R+P L   ++ +      S   Q S      
Sbjct: 447 QTSFLFQQASKVGFSHSNILRTIVHRACSRFPHLDWYNYGY------SQLLQGSTTLEVS 506

Query: 532 ESLRKVFVIFGGDTSERQVSLMSGTNVWLNLQVFDELEVTPCLLAP--TNEQTSSVDLDK 591
           E  +KVFVIFGGDTSERQVS+MSGTNVW+NLQ + +L VTPCLL+P  +N   +S +LD 
Sbjct: 507 EDPQKVFVIFGGDTSERQVSVMSGTNVWVNLQRYVDLNVTPCLLSPSLSNSLGASSNLD- 566

Query: 592 NEVDVSSRTVWSLPYSLVLRHTTEEVLAACIEAIEPTRAALTSRMRGLVVADLKEGLKKH 651
                 +R VW LPYS+VLRHT EEVLAAC+EA+EP RA  TS ++  V+ DL +G K  
Sbjct: 567 ------NREVWVLPYSVVLRHTAEEVLAACLEAVEPVRALFTSLLQKQVMEDLMDGFKNQ 626

Query: 652 SWFAGFDIKDELPVRFSLEQWIKQAKEVDATVFIAVHGGIGEDGSLQSLLEAKGVPYTGP 711
           SWFAGFDI DELP ++SL++WIK AKE  ATVFIAVHGGIGEDG+LQ LLE +GV YTGP
Sbjct: 627 SWFAGFDITDELPRKYSLKEWIKHAKEAQATVFIAVHGGIGEDGTLQGLLEDEGVSYTGP 686

Query: 712 GVVASSICMDKVATSLALNHLSDMGVLTIKKDVRRKDDLLDIPIINVWHDLTCKLQCQSL 771
           GV+AS  CMDKV TS AL++LS+ G+ TI KDV+R +D++     NVW +L  KLQC +L
Sbjct: 687 GVLASRTCMDKVMTSQALSNLSEFGIHTISKDVKRTEDIMHETFPNVWDELIKKLQCLTL 746

Query: 772 CVKPARDGCSTGVARLCCADDLAVYVKALKDCLARIPSNSFSKAHGMIEMPKPPPELLIC 831
           CVKPA+DGCSTGVARLC ++DLAVYV+ALKDC+ RIP N+ SK HGMIEMP P PE LI 
Sbjct: 747 CVKPAKDGCSTGVARLCSSEDLAVYVQALKDCIPRIPPNTLSKTHGMIEMPNPTPEFLIF 806

Query: 832 EPFIETDEIIVSSKSTNETEERLLWKGQSRWVEITVGVVGTRGSMHSLSPSVTVKEHGDI 891
           EPF+ETDEIIVSSK+    +++L WKG+ RWVE+TVGV+G RGSMHSLSPS+TVKE GDI
Sbjct: 807 EPFVETDEIIVSSKA----KQQLSWKGRRRWVEMTVGVIGKRGSMHSLSPSLTVKESGDI 866

Query: 892 LSLEEKFQGGTGINLTPPPLSIISNEALEKCKQHIELISNTLQLEGFSRIDAFVNVDSGE 951
           LSLEEKFQGGTGINLTPPP +I+S EALE+CKQ IELI+ TL LEGFSRIDAFV+V++GE
Sbjct: 867 LSLEEKFQGGTGINLTPPPPTIMSKEALERCKQGIELIAETLGLEGFSRIDAFVHVETGE 926

Query: 952 VLVIEVNTVPGMTPSTVLIHQALAETPPVYPHQFFRRLLDLASER 990
           VLVIEVNTVPGMTPSTVLI QALAE PP+YP QFFR LL LA++R
Sbjct: 927 VLVIEVNTVPGMTPSTVLIQQALAEQPPMYPPQFFRTLLHLATQR 935

BLAST of Sgr014428 vs. TAIR 10
Match: AT3G08840.3 (D-alanine--D-alanine ligase family )

HSP 1 Score: 993.0 bits (2566), Expect = 2.0e-289
Identity = 514/788 (65.23%), Postives = 607/788 (77.03%), Query Frame = 0

Query: 112 PSAERGISLNSARSVLDHIQGDDLQVSCYYIDSELNAYAISSAQVYSNTPADFDFKLKSL 171
           PSAERGISLNSARSVLDHIQGD + VSCYYID +L A+AISSAQVYSNTP+DFDFKL+SL
Sbjct: 87  PSAERGISLNSARSVLDHIQGDGINVSCYYIDPDLKAFAISSAQVYSNTPSDFDFKLESL 146

Query: 172 AHGFRSLTDFAEHLSASVDIVFPVIHGRFGEDGGIQELLERHNIPFVGTGSTECSRAFDK 231
           A GF SL++ AEHL ++VDIVFPVIHGRFGEDGGIQELLE HNIPFVGTGS EC RAFDK
Sbjct: 147 AQGFSSLSELAEHLVSAVDIVFPVIHGRFGEDGGIQELLESHNIPFVGTGSRECFRAFDK 206

Query: 232 YNASLELDRLGFITVPNFLVQAGSVNESELSQWFLRNQLDLSSGKVVVKPVRAGSSIGVT 291
           Y ASLEL  LGF+TVPN+LVQ   V++SE++ WF  NQLDL  GKVVVKP +AGSSIGV 
Sbjct: 207 YEASLELKELGFMTVPNYLVQGTGVDKSEIALWFTDNQLDLEMGKVVVKPAKAGSSIGVK 266

Query: 292 VAYGVVDSLKKANEIISKEIDDKVLVEVFLEGGSEFTAIVLDVGSGSVCHPVVLLPTEVE 351
           VA+GV DS+KKA E+I + IDD+V+VEVF+E   EFTAIVLDVGSGSVCHPVVL+PTEV+
Sbjct: 267 VAFGVNDSIKKATELILEGIDDRVVVEVFIEDAYEFTAIVLDVGSGSVCHPVVLMPTEVQ 326

Query: 352 LQFMVRLMWERRIQFSIIGGNIFPPSR-----YSDSVVPPILACLVAYHTPPRFPVDVIE 411
           LQF                  I  P       Y    +P      V YHTPPRFP+ VI+
Sbjct: 327 LQF----------------HGIGDPKENAIFDYRRKYLP---TQQVTYHTPPRFPIHVIK 386

Query: 412 SIREGASLLFKGLGLRDFARIDGWFLPNCCHESSYSTGNFGRTESGTVIYTDINLISGME 471
           SIRE ASL+F+ LGLRDFARIDGW+L    + SS  +   G T+SG +I+TDINLISGME
Sbjct: 387 SIREEASLIFQKLGLRDFARIDGWYLAPNSNLSSPVSETLGGTKSGDIIFTDINLISGME 446

Query: 472 QTSFLFQQASKVGFSHSNVLRSIIYHACLRYPSLGSLDHMFGNWPRRSNSSQRSQAYSSR 531
           QTSFLFQQASKVGFSHSN+LR+I++ AC R+P L   ++ +      S   Q S      
Sbjct: 447 QTSFLFQQASKVGFSHSNILRTIVHRACSRFPHLDWYNYGY------SQLLQGSTTLEVS 506

Query: 532 ESLRKVFVIFGGDTSERQVSLMSGTNVWLNLQVFDELEVTPCLLAP--TNEQTSSVDLDK 591
           E  +KVFVIFGGDTSERQVS+MSGTNVW+NLQ + +L VTPCLL+P  +N   +S +LD 
Sbjct: 507 EDPQKVFVIFGGDTSERQVSVMSGTNVWVNLQRYVDLNVTPCLLSPSLSNSLGASSNLD- 566

Query: 592 NEVDVSSRTVWSLPYSLVLRHTTEEVLAACIEAIEPTRAALTSRMRGLVVADLKEGLKKH 651
                 +R VW LPYS+VLRHT EEVLAAC+EA+EP RA  TS ++  V+ DL +G K  
Sbjct: 567 ------NREVWVLPYSVVLRHTAEEVLAACLEAVEPVRALFTSLLQKQVMEDLMDGFKNQ 626

Query: 652 SWFAGFDIKDELPVRFSLEQWIKQAKEVDATVFIAVHGGIGEDGSLQSLLEAKGVPYTGP 711
           SWFAGFDI DELP ++SL++WIK AKE  ATVFIAVHGGIGEDG+LQ LLE +GV YTGP
Sbjct: 627 SWFAGFDITDELPRKYSLKEWIKHAKEAQATVFIAVHGGIGEDGTLQGLLEDEGVSYTGP 686

Query: 712 GVVASSICMDKVATSLALNHLSDMGVLTIKKDVRRKDDLLDIPIINVWHDLTCKLQCQSL 771
           GV+AS  CMDKV TS AL++LS+ G+ TI KDV+R +D++     NVW +L  KLQC +L
Sbjct: 687 GVLASRTCMDKVMTSQALSNLSEFGIHTISKDVKRTEDIMHETFPNVWDELIKKLQCLTL 746

Query: 772 CVKPARDGCSTGVARLCCADDLAVYVKALKDCLARIPSNSFSKAHGMIEMPKPPPELLIC 831
           CVKPA+DGCSTGVARLC ++DLAVYV+ALKDC+ RIP N+ SK HGMIEMP P PE LI 
Sbjct: 747 CVKPAKDGCSTGVARLCSSEDLAVYVQALKDCIPRIPPNTLSKTHGMIEMPNPTPEFLIF 806

Query: 832 EPFIETDEIIVSSKSTNETEERLLWKGQSRWVEITVGVVGTRGSMHSLSPSVTVKEHGDI 891
           EPF+ETDEIIVSSK+    +++L WKG+ RWVE+TVGV+G RGSMHSLSPS+TVKE GDI
Sbjct: 807 EPFVETDEIIVSSKA----KQQLSWKGRRRWVEMTVGVIGKRGSMHSLSPSLTVKESGDI 838

Query: 892 LSLEEKFQ 893
           LSLEEKFQ
Sbjct: 867 LSLEEKFQ 838

BLAST of Sgr014428 vs. TAIR 10
Match: AT3G08840.1 (D-alanine--D-alanine ligase family )

HSP 1 Score: 562.0 bits (1447), Expect = 1.1e-159
Identity = 296/452 (65.49%), Postives = 343/452 (75.88%), Query Frame = 0

Query: 112 PSAERGISLNSARSVLDHIQGDDLQVSCYYIDSELNAYAISSAQVYSNTPADFDFKLKSL 171
           PSAERGISLNSARSVLDHIQGD + VSCYYID +L A+AISSAQVYSNTP+DFDFKL+SL
Sbjct: 87  PSAERGISLNSARSVLDHIQGDGINVSCYYIDPDLKAFAISSAQVYSNTPSDFDFKLESL 146

Query: 172 AHGFRSLTDFAEHLSASVDIVFPVIHGRFGEDGGIQELLERHNIPFVGTGSTECSRAFDK 231
           A GF SL++ AEHL ++VDIVFPVIHGRFGEDGGIQELLE HNIPFVGTGS EC RAFDK
Sbjct: 147 AQGFSSLSELAEHLVSAVDIVFPVIHGRFGEDGGIQELLESHNIPFVGTGSRECFRAFDK 206

Query: 232 YNASLELDRLGFITVPNFLVQAGSVNESELSQWFLRNQLDLSSGKVVVKPVRAGSSIGVT 291
           Y ASLEL  LGF+TVPN+LVQ   V++SE++ WF  NQLDL  GKVVVKP +AGSSIGV 
Sbjct: 207 YEASLELKELGFMTVPNYLVQGTGVDKSEIALWFTDNQLDLEMGKVVVKPAKAGSSIGVK 266

Query: 292 VAYGVVDSLKKANEIISKEIDDKVLVEVFLEGGSEFTAIVLDVGSGSVCHPVVLLPTEVE 351
           VA+GV DS+KKA E+I + IDD+V+VEVF+E   EFTAIVLDVGSGSVCHPVVL+PTEV+
Sbjct: 267 VAFGVNDSIKKATELILEGIDDRVVVEVFIEDAYEFTAIVLDVGSGSVCHPVVLMPTEVQ 326

Query: 352 LQFMVRLMWERRIQFSIIGGNIFPPSR-----YSDSVVPPILACLVAYHTPPRFPVDVIE 411
           LQF                  I  P       Y    +P      V YHTPPRFP+ VI+
Sbjct: 327 LQF----------------HGIGDPKENAIFDYRRKYLP---TQQVTYHTPPRFPIHVIK 386

Query: 412 SIREGASLLFKGLGLRDFARIDGWFLPNCCHESSYSTGNFGRTESGTVIYTDINLISGME 471
           SIRE ASL+F+ LGLRDFARIDGW+L    + SS  +   G T+SG +I+TDINLISGME
Sbjct: 387 SIREEASLIFQKLGLRDFARIDGWYLAPNSNLSSPVSETLGGTKSGDIIFTDINLISGME 446

Query: 472 QTSFLFQQASKVGFSHSNVLRSIIYHACLRYPSLGSLDHMFGNWPRRSNSSQRSQAYSSR 531
           QTSFLFQQASKVGFSHSN+LR+I++ AC R+P L   ++ +      S   Q S      
Sbjct: 447 QTSFLFQQASKVGFSHSNILRTIVHRACSRFPHLDWYNYGY------SQLLQGSTTLEVS 506

Query: 532 ESLRKVFVIFGGDTSERQVSLMSGTNVWLNLQ 559
           E  +KVFVIFGGDTSERQVS+MSGTNVW+NLQ
Sbjct: 507 EDPQKVFVIFGGDTSERQVSVMSGTNVWVNLQ 513