Sgr014327 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr014327
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Descriptionwall-associated receptor kinase 2-like
Locationtig00000289: 517728 .. 520201 (+)
RNA-Seq ExpressionSgr014327
SyntenySgr014327
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGACGTCCAACGGAGAAGCTTGTTCGACTCATGACGTTGTTGTCAACGGCTTTAGTAGCGACGGCGGCGACGGGGTCATCGGACTGCGAACGACGGTGTGGGAATTTGGAAATTCCATATCCGTTCGGGATGAAGGAAGGGTGTTATCTGAATAAAAATTTCTCCATTACCTGCAACAAAACTCATTATGATCCTCCAAAGGCATTTCTAGGAGACGGCAACATTGATTTAACTAACATATCCATCATCCACGGCGAGCTCCGCATCTTGCAGTACGTAGCCCGAGATTGCTACACACAAAATGGTTCCAATATCTTCAACAATCCCACTATCAATATATCCGAGTTCACCATTTCCCACACTAAAAACAAGTTCACGGTCATCGGCTGCGATACTTACGCTTTTATTTCCGGCCAACTGGAGGGGGATTCCTACAGAAGTGGGTGCATGTCGTTATGTGGAAACAGTACTACCACTATAAGAGATGGGTCCTGCTCTGGCAATGGGTGCTGTCAGTTGGAAATTCCCAAAGGCCTAAAAGATTTGAACTTGAAGGTGAATAGCTTTGAAAATCACACTCACGTACTCAACTTCAATCCTTGTGGGTATGCTTTTGTAATCCAACAAGATAACTTCACTTTCTCCTCCACATATATTCGAAATTTTACACAAGAGGAAGTTCCGATGGTGCTTGATTGGGCCATCCCTACAGATACTTGCTCAACAGCTGACGACAAAACCAATTGCATATGTGGACCAAACAGCAAAAAGATTGACTTTCTCAATGATGGATCTGAATATCGTTGCCAGTGCTTGGATGGTTTCGAGGGGAATCCATATCTCCCTCAAGGTTGTCAAGGTAAAATTCGTAATGCTACTTTTACATGGTTAACTCACATTAATTTGGCATTTAAAAAAAAAATCAAAATGTTAATTTTATTGTTAAATTTGGTGCATTTTCAGATATAGATGAATGCAAGAATGGAAGCCATGAATGTAAATTCATAGACAAGTGTGTGAACACACCAGGAAACTATACCTGCAATTGTCCTAAGAACTATAAAGGAGATGGCAAACGTGGAGGAGAAGGTTGCACCCCAAACTCCAAGTCTTTCATTCAGATCATCATCGGTAAGTTTTTATATTTTAATACCAAATTTGTATTATATTATAATTTGAAAGGTTTTTTTTTTCTTTTCTTTAATATTCGTATGTTATTACATGCTTTTGATAACAATATTACTAAAAAGAAACAGAAATATTAATGTATACTACTGAAATAAAATAATGCAGGAATTGGTGTGGGGTTCATAGTTTTAGTGATTGCTAGTACATGGTTATACTTGATTTACAAAAAGTGGAAGTTTATCCAACGGAAAGAGAAGTTTTTTGAGAAAAATGGAGGCTTCATGCTTCAACATCATCTTTCTCAGCGGCAATCACCTGCCGACATGCTCAGAATTTTCACCCAAGAAGAGTTGGAGAAGGCTACAAACAAGTACGATGAAAGCGCAGTGGTTGGAAGAGGTGGCTTCGGTACGGTTTATAAAGGAGTCTTAGACGACGGTCTCACAGTCGCAATCAAGAAATCAAAAATGGTGGACCAATCCCAAACTGCCCAATTTATTAACGAAGTCATCGTTCTCTCCCAAATCAACCATCGGAACGTGGTCAAGCTCTTGGGTTGCTGTTTGGAGACACAAGTTCCATTGCTGGTCTACGAGTTCATCACCAACGGCACACTCTTCGACCACATCCATGACAAAATCAAGCATGTTTCTCTTTCATGGGAAGCTCGCTTGAAAATAGCTTCGGAAACTGCGGGCGTCCTTTCATATTTGCATTCTTCAGCTTCTACTCCAATCATCCACAGAGATATCAAGTCGACTAATATACTGTTAGACGATAATTGCACTGCAAAAGTCTCTGATTTCGGAGCTTCGAAGCTGGTTCCACTGGATCATACTCAGATATCCACGATGGTGCAAGGGACTCTTGGATATTTGGACCCAGAATATTTGTTGACGAGTGAGTTGACGGAGAAAAGCGATGTGTACAGCTTTGGAATTGTGCTTCTGGAGCTTATAACTGGAAAGAAAGCGGTGTGTTTTGCTGGGCCCGAAGCAGAGAGAAATTTGGCCATGTATGTCCTCTGTGCAATGAAAGAAGATCGTTTGGAAGAAGTTGTTGAGAAGGAAATGGCAACGGAGGGGAACTTTGAGCAGATAAAAGAAGTGGCCAAGGTAGCAAAAGAGTGTGTGAGAGTAAAAGGGGAGGAGCGACCCAGCATGAAGGAGGTAGCTATGGAGTTGGAGGGGCTGCGACTGAAGCAGGTTGAGCATTTATGGGCTAAGAACAGTTTGTTGGGTGGAGCTTTAGGTACAAGCCAACTTGTCGTCAGTGGGGGTATGAATGCTATGGACAATAGCATAAAGGATCAGATTTTATCACACATCCCCCATGGAAGATGA

mRNA sequence

ATGGGACGTCCAACGGAGAAGCTTGTTCGACTCATGACGTTGTTGTCAACGGCTTTAGTAGCGACGGCGGCGACGGGGTCATCGGACTGCGAACGACGGTGTGGGAATTTGGAAATTCCATATCCGTTCGGGATGAAGGAAGGGTGTTATCTGAATAAAAATTTCTCCATTACCTGCAACAAAACTCATTATGATCCTCCAAAGGCATTTCTAGGAGACGGCAACATTGATTTAACTAACATATCCATCATCCACGGCGAGCTCCGCATCTTGCAGTACGTAGCCCGAGATTGCTACACACAAAATGGTTCCAATATCTTCAACAATCCCACTATCAATATATCCGAGTTCACCATTTCCCACACTAAAAACAAGTTCACGGTCATCGGCTGCGATACTTACGCTTTTATTTCCGGCCAACTGGAGGGGGATTCCTACAGAAGTGGGTGCATGTCGTTATGTGGAAACAGTACTACCACTATAAGAGATGGGTCCTGCTCTGGCAATGGGTGCTGTCAGTTGGAAATTCCCAAAGGCCTAAAAGATTTGAACTTGAAGGTGAATAGCTTTGAAAATCACACTCACGTACTCAACTTCAATCCTTGTGGGTATGCTTTTGTAATCCAACAAGATAACTTCACTTTCTCCTCCACATATATTCGAAATTTTACACAAGAGGAAGTTCCGATGGTGCTTGATTGGGCCATCCCTACAGATACTTGCTCAACAGCTGACGACAAAACCAATTGCATATGTGGACCAAACAGCAAAAAGATTGACTTTCTCAATGATGGATCTGAATATCGTTGCCAGTGCTTGGATGGTTTCGAGGGGAATCCATATCTCCCTCAAGGTTGTCAAGATATAGATGAATGCAAGAATGGAAGCCATGAATGTAAATTCATAGACAAGTGTGTGAACACACCAGGAAACTATACCTGCAATTGTCCTAAGAACTATAAAGGAGATGGCAAACGTGGAGGAGAAGGTTGCACCCCAAACTCCAAGTCTTTCATTCAGATCATCATCGGAATTGGTGTGGGGTTCATAGTTTTAGTGATTGCTAGTACATGGTTATACTTGATTTACAAAAAGTGGAAGTTTATCCAACGGAAAGAGAAGTTTTTTGAGAAAAATGGAGGCTTCATGCTTCAACATCATCTTTCTCAGCGGCAATCACCTGCCGACATGCTCAGAATTTTCACCCAAGAAGAGTTGGAGAAGGCTACAAACAAGTACGATGAAAGCGCAGTGGTTGGAAGAGGTGGCTTCGGTACGGTTTATAAAGGAGTCTTAGACGACGGTCTCACAGTCGCAATCAAGAAATCAAAAATGGTGGACCAATCCCAAACTGCCCAATTTATTAACGAAGTCATCGTTCTCTCCCAAATCAACCATCGGAACGTGGTCAAGCTCTTGGGTTGCTGTTTGGAGACACAAGTTCCATTGCTGGTCTACGAGTTCATCACCAACGGCACACTCTTCGACCACATCCATGACAAAATCAAGCATGTTTCTCTTTCATGGGAAGCTCGCTTGAAAATAGCTTCGGAAACTGCGGGCGTCCTTTCATATTTGCATTCTTCAGCTTCTACTCCAATCATCCACAGAGATATCAAGTCGACTAATATACTGTTAGACGATAATTGCACTGCAAAAGTCTCTGATTTCGGAGCTTCGAAGCTGGTTCCACTGGATCATACTCAGATATCCACGATGGTGCAAGGGACTCTTGGATATTTGGACCCAGAATATTTGTTGACGAGTGAGTTGACGGAGAAAAGCGATGTGTACAGCTTTGGAATTGTGCTTCTGGAGCTTATAACTGGAAAGAAAGCGGTGTGTTTTGCTGGGCCCGAAGCAGAGAGAAATTTGGCCATGTATGTCCTCTGTGCAATGAAAGAAGATCGTTTGGAAGAAGTTGTTGAGAAGGAAATGGCAACGGAGGGGAACTTTGAGCAGATAAAAGAAGTGGCCAAGGTAGCAAAAGAGTGTGTGAGAGTAAAAGGGGAGGAGCGACCCAGCATGAAGGAGGTAGCTATGGAGTTGGAGGGGCTGCGACTGAAGCAGGTTGAGCATTTATGGGCTAAGAACAGTTTGTTGGGTGGAGCTTTAGGTACAAGCCAACTTGTCGTCAGTGGGGGTATGAATGCTATGGACAATAGCATAAAGGATCAGATTTTATCACACATCCCCCATGGAAGATGA

Coding sequence (CDS)

ATGGGACGTCCAACGGAGAAGCTTGTTCGACTCATGACGTTGTTGTCAACGGCTTTAGTAGCGACGGCGGCGACGGGGTCATCGGACTGCGAACGACGGTGTGGGAATTTGGAAATTCCATATCCGTTCGGGATGAAGGAAGGGTGTTATCTGAATAAAAATTTCTCCATTACCTGCAACAAAACTCATTATGATCCTCCAAAGGCATTTCTAGGAGACGGCAACATTGATTTAACTAACATATCCATCATCCACGGCGAGCTCCGCATCTTGCAGTACGTAGCCCGAGATTGCTACACACAAAATGGTTCCAATATCTTCAACAATCCCACTATCAATATATCCGAGTTCACCATTTCCCACACTAAAAACAAGTTCACGGTCATCGGCTGCGATACTTACGCTTTTATTTCCGGCCAACTGGAGGGGGATTCCTACAGAAGTGGGTGCATGTCGTTATGTGGAAACAGTACTACCACTATAAGAGATGGGTCCTGCTCTGGCAATGGGTGCTGTCAGTTGGAAATTCCCAAAGGCCTAAAAGATTTGAACTTGAAGGTGAATAGCTTTGAAAATCACACTCACGTACTCAACTTCAATCCTTGTGGGTATGCTTTTGTAATCCAACAAGATAACTTCACTTTCTCCTCCACATATATTCGAAATTTTACACAAGAGGAAGTTCCGATGGTGCTTGATTGGGCCATCCCTACAGATACTTGCTCAACAGCTGACGACAAAACCAATTGCATATGTGGACCAAACAGCAAAAAGATTGACTTTCTCAATGATGGATCTGAATATCGTTGCCAGTGCTTGGATGGTTTCGAGGGGAATCCATATCTCCCTCAAGGTTGTCAAGATATAGATGAATGCAAGAATGGAAGCCATGAATGTAAATTCATAGACAAGTGTGTGAACACACCAGGAAACTATACCTGCAATTGTCCTAAGAACTATAAAGGAGATGGCAAACGTGGAGGAGAAGGTTGCACCCCAAACTCCAAGTCTTTCATTCAGATCATCATCGGAATTGGTGTGGGGTTCATAGTTTTAGTGATTGCTAGTACATGGTTATACTTGATTTACAAAAAGTGGAAGTTTATCCAACGGAAAGAGAAGTTTTTTGAGAAAAATGGAGGCTTCATGCTTCAACATCATCTTTCTCAGCGGCAATCACCTGCCGACATGCTCAGAATTTTCACCCAAGAAGAGTTGGAGAAGGCTACAAACAAGTACGATGAAAGCGCAGTGGTTGGAAGAGGTGGCTTCGGTACGGTTTATAAAGGAGTCTTAGACGACGGTCTCACAGTCGCAATCAAGAAATCAAAAATGGTGGACCAATCCCAAACTGCCCAATTTATTAACGAAGTCATCGTTCTCTCCCAAATCAACCATCGGAACGTGGTCAAGCTCTTGGGTTGCTGTTTGGAGACACAAGTTCCATTGCTGGTCTACGAGTTCATCACCAACGGCACACTCTTCGACCACATCCATGACAAAATCAAGCATGTTTCTCTTTCATGGGAAGCTCGCTTGAAAATAGCTTCGGAAACTGCGGGCGTCCTTTCATATTTGCATTCTTCAGCTTCTACTCCAATCATCCACAGAGATATCAAGTCGACTAATATACTGTTAGACGATAATTGCACTGCAAAAGTCTCTGATTTCGGAGCTTCGAAGCTGGTTCCACTGGATCATACTCAGATATCCACGATGGTGCAAGGGACTCTTGGATATTTGGACCCAGAATATTTGTTGACGAGTGAGTTGACGGAGAAAAGCGATGTGTACAGCTTTGGAATTGTGCTTCTGGAGCTTATAACTGGAAAGAAAGCGGTGTGTTTTGCTGGGCCCGAAGCAGAGAGAAATTTGGCCATGTATGTCCTCTGTGCAATGAAAGAAGATCGTTTGGAAGAAGTTGTTGAGAAGGAAATGGCAACGGAGGGGAACTTTGAGCAGATAAAAGAAGTGGCCAAGGTAGCAAAAGAGTGTGTGAGAGTAAAAGGGGAGGAGCGACCCAGCATGAAGGAGGTAGCTATGGAGTTGGAGGGGCTGCGACTGAAGCAGGTTGAGCATTTATGGGCTAAGAACAGTTTGTTGGGTGGAGCTTTAGGTACAAGCCAACTTGTCGTCAGTGGGGGTATGAATGCTATGGACAATAGCATAAAGGATCAGATTTTATCACACATCCCCCATGGAAGATGA

Protein sequence

MGRPTEKLVRLMTLLSTALVATAATGSSDCERRCGNLEIPYPFGMKEGCYLNKNFSITCNKTHYDPPKAFLGDGNIDLTNISIIHGELRILQYVARDCYTQNGSNIFNNPTINISEFTISHTKNKFTVIGCDTYAFISGQLEGDSYRSGCMSLCGNSTTTIRDGSCSGNGCCQLEIPKGLKDLNLKVNSFENHTHVLNFNPCGYAFVIQQDNFTFSSTYIRNFTQEEVPMVLDWAIPTDTCSTADDKTNCICGPNSKKIDFLNDGSEYRCQCLDGFEGNPYLPQGCQDIDECKNGSHECKFIDKCVNTPGNYTCNCPKNYKGDGKRGGEGCTPNSKSFIQIIIGIGVGFIVLVIASTWLYLIYKKWKFIQRKEKFFEKNGGFMLQHHLSQRQSPADMLRIFTQEELEKATNKYDESAVVGRGGFGTVYKGVLDDGLTVAIKKSKMVDQSQTAQFINEVIVLSQINHRNVVKLLGCCLETQVPLLVYEFITNGTLFDHIHDKIKHVSLSWEARLKIASETAGVLSYLHSSASTPIIHRDIKSTNILLDDNCTAKVSDFGASKLVPLDHTQISTMVQGTLGYLDPEYLLTSELTEKSDVYSFGIVLLELITGKKAVCFAGPEAERNLAMYVLCAMKEDRLEEVVEKEMATEGNFEQIKEVAKVAKECVRVKGEERPSMKEVAMELEGLRLKQVEHLWAKNSLLGGALGTSQLVVSGGMNAMDNSIKDQILSHIPHGR
Homology
BLAST of Sgr014327 vs. NCBI nr
Match: XP_038885912.1 (wall-associated receptor kinase 2-like [Benincasa hispida])

HSP 1 Score: 1065.8 bits (2755), Expect = 1.6e-307
Identity = 534/727 (73.45%), Postives = 611/727 (84.04%), Query Frame = 0

Query: 1   MGRPTEKLVRLMTLLSTALVAT---AATGSSDCERRCGNLEIPYPFGMKEGCYLNKNFSI 60
           M   TE L++L+ LLS  L A    AA G + C+ RCG+L+IPYPFG+K+GCYL+++FSI
Sbjct: 1   MRSATENLIKLIILLSATLAAAAPEAAKGLTGCDERCGDLKIPYPFGIKKGCYLDEDFSI 60

Query: 61  TCNKTHYDPPKAFLGDGNIDLTNISIIHGELRILQYVARDCYTQNGSNIFNNPTINISEF 120
           TCNK+H DPPKAFL D N D+TNISII G+L ILQ+VA+DCYT++G +    PT+ + + 
Sbjct: 61  TCNKSH-DPPKAFLKDSNTDITNISIIQGQLHILQFVAKDCYTKDGPSEAYTPTLELYDL 120

Query: 121 TISHTKNKFTVIGCDTYAFISGQLEGDSYRSGCMSLCGNSTTTIRDGSCSGNGCCQLEIP 180
           TIS+T NKF VIGCDTYAFISG++EG SY+SGCM+LCGNST TI+DGSCSGNGCCQLEIP
Sbjct: 121 TISNTDNKFIVIGCDTYAFISGEIEGQSYKSGCMALCGNSTKTIKDGSCSGNGCCQLEIP 180

Query: 181 KGLKDLNLKVNSFENHTHVLNFNPCGYAFVIQQDNFTFSSTYIRNFTQEEVPMVLDWAIP 240
           KGLK L L V SF NH+ VL FN CGYAFV+QQD FTFS  YI NFTQEEVP+VLDWAIP
Sbjct: 181 KGLKYLELDVRSFNNHSEVLEFNSCGYAFVVQQDKFTFSEKYIHNFTQEEVPLVLDWAIP 240

Query: 241 TDT-CSTADDKTNC-ICGPNSKKIDFLNDGSEYRCQCLDGFEGNPYLPQGCQDIDECKNG 300
            +T C  A +KTNC ICG N+K I FL+DGS YRCQCL+GFEGNPYLPQGCQDIDECKNG
Sbjct: 241 ANTSCLKAGNKTNCSICGTNTKTISFLDDGSNYRCQCLEGFEGNPYLPQGCQDIDECKNG 300

Query: 301 SHECKFIDKCVNTPGNYTCNCPKNYKGDGKRGGEGCTPNSKSFIQIIIGIGVGFIVLVIA 360
           SH+C + D CVNT GNYTC CPKN++GDG+ GGEGCTPNS+S I IIIGI VGF VLVI 
Sbjct: 301 SHKCTYKDLCVNTQGNYTCYCPKNHEGDGRYGGEGCTPNSRSSIHIIIGICVGFAVLVIG 360

Query: 361 STWLYLIYKKWKFIQRKEKFFEKNGGFMLQHHLSQRQSPADMLRIFTQEELEKATNKYDE 420
           S WLYL YKKWKFIQ+KEKFF+KNGG MLQ HLS+ QSP DML+IFTQE+LEKATNKYD+
Sbjct: 361 SVWLYLGYKKWKFIQQKEKFFKKNGGLMLQKHLSEWQSP-DMLKIFTQEKLEKATNKYDD 420

Query: 421 SAVVGRGGFGTVYKGVLDDGLTVAIKKSKMVDQSQTAQFINEVIVLSQINHRNVVKLLGC 480
           SA++G+GGFGTVYKGVLDDG TVAIKKSK+V+QSQT QFINEVIVLSQINHRNVVKL+GC
Sbjct: 421 SAMIGKGGFGTVYKGVLDDGSTVAIKKSKLVNQSQTNQFINEVIVLSQINHRNVVKLIGC 480

Query: 481 CLETQVPLLVYEFITNGTLFDHIHDKIKHVSLSWEARLKIASETAGVLSYLHSSASTPII 540
           CLET+VPLLVYEF+ N TLF+HIH +     L WEARLKIASE A VLSYLHSS STPII
Sbjct: 481 CLETKVPLLVYEFVNNRTLFEHIHRRTNQAYLPWEARLKIASEIASVLSYLHSSTSTPII 540

Query: 541 HRDIKSTNILLDDNCTAKVSDFGASKLVPLDHTQISTMVQGTLGYLDPEYLLTSELTEKS 600
           HRDIKS NILLD N TAKVSDFG SKLVPLD TQISTMVQGT+GYLDPEY LTSELTEKS
Sbjct: 541 HRDIKSNNILLDQNYTAKVSDFGMSKLVPLDGTQISTMVQGTIGYLDPEYYLTSELTEKS 600

Query: 601 DVYSFGIVLLELITGKKAVCFAGPEAERNLAMYVLCAMKEDRLEEVVEKEMATEGNFEQI 660
           DVYSFGIVL+ELITGKKAVCF GPE ER+LAMYVL AM+EDR+EEVVEK M TE NFEQI
Sbjct: 601 DVYSFGIVLMELITGKKAVCFDGPEEERSLAMYVLYAMEEDRVEEVVEKGMPTEKNFEQI 660

Query: 661 KEVAKVAKECVRVKGEERPSMKEVAMELEGLR-LKQVE-HLWAKNSLLGGALGTSQLVVS 720
           K+VA++ +ECVRVK EERPSMKEVAM+LE LR ++QVE + W +++LL G   +SQLV  
Sbjct: 661 KKVAELGRECVRVKKEERPSMKEVAMKLEVLRQMQQVEFYSWGRDNLLDGVSNSSQLVTI 720

BLAST of Sgr014327 vs. NCBI nr
Match: XP_038886240.1 (wall-associated receptor kinase 2-like [Benincasa hispida])

HSP 1 Score: 1065.1 bits (2753), Expect = 2.8e-307
Identity = 532/745 (71.41%), Postives = 613/745 (82.28%), Query Frame = 0

Query: 1   MGRPTEKLVRLMTLL--STALVATAATGSSDCERRCGNLEIPYPFGMKEGCYLNKNFSIT 60
           M  P EKL+ L  L+   +  +ATAA  S DCER+CGNLEIPYPFGMK+GCYLN+NF +T
Sbjct: 1   MEHPEEKLLFLFMLIMFPSVALATAAAASQDCERQCGNLEIPYPFGMKKGCYLNENFLVT 60

Query: 61  CNKTHYDPPKAFLGDGNIDLTNISIIHGELRILQYVARDCYTQNGSNIFN--NPTINISE 120
           CNKTHY PPKAFL   NI++T+ISI+  EL IL YVARDCYT++G    +   P + +  
Sbjct: 61  CNKTHYHPPKAFLRGSNIEVTHISILPSELHILNYVARDCYTKDGRPDISRARPFLVVDM 120

Query: 121 FTISHTKNKFTVIGCDTYAFISGQLEGDSYRSGCMSLCGNSTTTIRDGSCSGNGCCQLEI 180
           F+IS+TKNK T++GCDTY ++ G+++G+ Y SGCM+LCGNS+ TI+DGSCSG+GCCQLEI
Sbjct: 121 FSISNTKNKLTIVGCDTYGYLHGEIQGEIYSSGCMALCGNSSRTIKDGSCSGSGCCQLEI 180

Query: 181 PKGLKDLNLKVNSFENHTHVLNFNPCGYAFVIQQDNFTFSSTYIRNFTQEEVPMVLDWAI 240
           PKGLK++ L V SF NHT V + NPCGYAFVIQQ+ FTFS  YI+NFT+ +VP+VLDW I
Sbjct: 181 PKGLKEIQLDVRSFNNHTRVHSLNPCGYAFVIQQNRFTFSKNYIKNFTESKVPLVLDWGI 240

Query: 241 PTDTCSTADDKTNCICGPNSKKIDFLN-DGSEYRCQCLDGFEGNPYLPQGCQDIDECKNG 300
             DTC   + K  C+CGPNS+K   L+ DGSEY CQCLDGF GNPYL QGCQDIDEC++G
Sbjct: 241 KNDTCQPPNHKDKCLCGPNSQKNSSLSLDGSEYYCQCLDGFHGNPYLSQGCQDIDECQDG 300

Query: 301 SHECKFIDKCVNTPGNYTCNCPKNYKGDGKRGGEGCTPNSKSFIQIIIGIGVGFIVLVIA 360
           SHECKF  +CVNTPGNYTCNCPKNYKGDG+RGGEGCT N+  FI IIIGI VG +VL I+
Sbjct: 301 SHECKFKHQCVNTPGNYTCNCPKNYKGDGRRGGEGCTQNTTFFILIIIGIVVGLLVLGIS 360

Query: 361 STWLYLIYKKWKFIQRKEKFFEKNGGFMLQHHLSQRQSPADMLRIFTQEELEKATNKYDE 420
           S WLYL YKKW+FIQ+K KFF KNGG +LQ H+SQ QS +DMLRIFT+EELEKATN +DE
Sbjct: 361 SMWLYLAYKKWRFIQQKAKFFNKNGGLVLQEHISQWQSSSDMLRIFTREELEKATNNFDE 420

Query: 421 SAVVGRGGFGTVYKGVLDDGLTVAIKKSKMVDQSQTAQFINEVIVLSQINHRNVVKLLGC 480
           S VVG+GG+GTVYKGVL DG  +AIKKSK+VDQSQT QFINEVI+LSQINHRNVVKLLGC
Sbjct: 421 SVVVGKGGYGTVYKGVLMDGSIIAIKKSKLVDQSQTKQFINEVIILSQINHRNVVKLLGC 480

Query: 481 CLETQVPLLVYEFITNGTLFDHIHDKIKHVSLSWEARLKIASETAGVLSYLHSSASTPII 540
           CLET+VPLLVYEFITNGTLF+HIH K  H  LSW+ RLKIAS+TAGVLSYLHSSASTPII
Sbjct: 481 CLETRVPLLVYEFITNGTLFEHIHRKNNHSFLSWKTRLKIASQTAGVLSYLHSSASTPII 540

Query: 541 HRDIKSTNILLDDNCTAKVSDFGASKLVPLDHTQISTMVQGTLGYLDPEYLLTSELTEKS 600
           HRDIKSTNILLD N TAKVSDFGASKLVPLD TQISTMVQGTLGYLDPEYLLTSELTEKS
Sbjct: 541 HRDIKSTNILLDHNYTAKVSDFGASKLVPLDQTQISTMVQGTLGYLDPEYLLTSELTEKS 600

Query: 601 DVYSFGIVLLELITGKKAVCFAGPEAERNLAMYVLCAMKEDRLEEVVEKEM--ATEGNFE 660
           DVYSFGIVLLELITGKKAV F GPE ERNLAMYVLCA+KEDR+EE+VE E+    EG F 
Sbjct: 601 DVYSFGIVLLELITGKKAVTFTGPELERNLAMYVLCAIKEDRVEEIVENEIMETEEGVFW 660

Query: 661 QIKEVAKVAKECVRVKGEERPSMKEVAMELEGLRLKQVEHLWAKNSLLGGALGTSQLVV- 720
           QIKEV K+AKEC+RVKGEERP+MKEVAMEL+ LR+ QVEHL    +L   A  ++ + V 
Sbjct: 661 QIKEVTKLAKECLRVKGEERPTMKEVAMELDRLRVMQVEHL----NLSDEASDSTSINVG 720

Query: 721 --SGGMNAMDNSIKDQILSHIPHGR 736
             +   NAMD+SIK QILS IP GR
Sbjct: 721 CDNMNNNAMDHSIKAQILSRIPLGR 741

BLAST of Sgr014327 vs. NCBI nr
Match: XP_022989770.1 (wall-associated receptor kinase 2-like [Cucurbita maxima])

HSP 1 Score: 1059.7 bits (2739), Expect = 1.2e-305
Identity = 526/742 (70.89%), Postives = 611/742 (82.35%), Query Frame = 0

Query: 1   MGRPTEKLVRLM----TLLSTALVATAATGSSDCERRCGNLEIPYPFGMKEGCYLNKNFS 60
           MGR  +  +RLM     +LS ++ A A+     C+ RCG+L+IPYPFG +EGCYLNKNF 
Sbjct: 1   MGRQIKTHIRLMIINILILSPSVYAVASQALLGCDDRCGDLQIPYPFGTREGCYLNKNFL 60

Query: 61  ITCNKTHYDPPKAFLGDGNIDLTNISIIHGELRILQYVARDCYTQNGS--NIFNNPTINI 120
           ITCN TH+DPP+ FL  GNI +TNIS I GEL+IL + A+DCY +N S      + T+N+
Sbjct: 61  ITCNTTHFDPPRPFLRTGNIQVTNIS-ISGELQILHFGAKDCYPKNNSLDARRTSATLNL 120

Query: 121 SEFTISHTKNKFTVIGCDTYAFISGQLEGDSYRSGCMSLCGNSTTTIRDGSCSGNGCCQL 180
           S FT+S TKNKFTVIGCDTYAF+SGQ+EG SYR+ C++LC N  TT+RDG+CSGNGCCQL
Sbjct: 121 STFTVSSTKNKFTVIGCDTYAFLSGQIEGQSYRTACVALCDN-ITTVRDGACSGNGCCQL 180

Query: 181 EIPKGLKDLNLKVNSFENHTHVLNFNPCGYAFVIQQDNFTFSSTYIRNFTQEEVPMVLDW 240
           +IP GLK L  +V SF+NHT V +FNPCGYAFV ++D F FS+ YIRNF Q +VP+VLDW
Sbjct: 181 DIPSGLKGLRYRVRSFDNHTDVFSFNPCGYAFVTEEDKFHFSAAYIRNFPQRQVPVVLDW 240

Query: 241 AIPTDTCSTADDKTNCICGPNSKKIDFLNDGSEYRCQCLDGFEGNPYLPQGCQDIDECKN 300
            I   TCSTA++K+NCICGPNS  ++ L DGSEYRC CLDGFEGNPYLP+GCQDIDEC++
Sbjct: 241 GISNTTCSTANNKSNCICGPNSMMVNVLPDGSEYRCGCLDGFEGNPYLPRGCQDIDECRD 300

Query: 301 GS-HECKFIDKCVNTPGNYTCNCPKNYKGDGKRGGEGCTPNSKSFIQIIIGIGVGFIVLV 360
              ++CKF  +CVNT GNYTCNCP+ +KGDG+RGGEGCT +SKSF+Q+IIG+ VGF VLV
Sbjct: 301 ERLNDCKF--ECVNTKGNYTCNCPEGFKGDGRRGGEGCTRSSKSFVQVIIGVSVGFTVLV 360

Query: 361 IASTWLYLIYKKWKFIQRKEKFFEKNGGFMLQHHLSQRQSPADMLRIFTQEELEKATNKY 420
           I STWLYL Y+KWK I+ KEKFFE+NGG MLQ HLSQ +S  D + IFTQEEL+KATNKY
Sbjct: 361 IGSTWLYLGYRKWKLIKLKEKFFEENGGLMLQRHLSQWKSSTDTVTIFTQEELDKATNKY 420

Query: 421 DESAVVGRGGFGTVYKGVLDDGLTVAIKKSKMVDQSQTAQFINEVIVLSQINHRNVVKLL 480
           DESAV+G+GG+GTVYKG+L DG  VAIKKSK+VDQSQT+QFINEVIVLSQINHRNVVKLL
Sbjct: 421 DESAVIGKGGYGTVYKGILPDGSVVAIKKSKLVDQSQTSQFINEVIVLSQINHRNVVKLL 480

Query: 481 GCCLETQVPLLVYEFITNGTLFDHIHDKIKHVSLSWEARLKIASETAGVLSYLHSSASTP 540
           GCCLETQVPLLVYEF+TNGTLFDHIHD  KHV LSW+ARL+IA ETAGV+SYLHSSASTP
Sbjct: 481 GCCLETQVPLLVYEFVTNGTLFDHIHDTTKHVPLSWKARLRIALETAGVISYLHSSASTP 540

Query: 541 IIHRDIKSTNILLDDNCTAKVSDFGASKLVPLDHTQISTMVQGTLGYLDPEYLLTSELTE 600
           IIHRDIK+TNILLDDN  AKVSDFGASKLVPLD TQ+STMVQGTLGYLDPEYLLTSELTE
Sbjct: 541 IIHRDIKTTNILLDDNYNAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTE 600

Query: 601 KSDVYSFGIVLLELITGKKAVCFAGPEAERNLAMYVLCAMKEDRLEEVVEKEMATEGNFE 660
           KSDVYSFGIVLLELITGKKAV F GPEAERNLAMYVLCAMKEDRL EVVEK MA EG FE
Sbjct: 601 KSDVYSFGIVLLELITGKKAVSFEGPEAERNLAMYVLCAMKEDRLGEVVEKGMAREGEFE 660

Query: 661 QIKEVAKVAKECVRVKGEERPSMKEVAMELEGLRLKQVEHLWAKNSLLGGALGTSQLVVS 720
           QIK+V KVA++C+R+ GEERPSMKEV MELEGLR+   EH W     L     ++  VVS
Sbjct: 661 QIKDVGKVARKCLRISGEERPSMKEVVMELEGLRV-MAEHEWVDEENLVADGASNIFVVS 720

Query: 721 GGMNAMDNSIKDQILSHIPHGR 736
           G  N +D+S+K Q+L  I  GR
Sbjct: 721 GSTNVVDDSMKVQVLPLIHDGR 737

BLAST of Sgr014327 vs. NCBI nr
Match: XP_023512416.1 (wall-associated receptor kinase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1058.9 bits (2737), Expect = 2.0e-305
Identity = 531/742 (71.56%), Postives = 612/742 (82.48%), Query Frame = 0

Query: 1   MGRPTEKLVRLM----TLLSTALVATAATGSSDCERRCGNLEIPYPFGMKEGCYLNKNFS 60
           M R  E L+RLM     +LS+ + A+ A     C  RCG+L+IPYPFG +EGCYLNKNF 
Sbjct: 1   MERQIETLIRLMIVNILILSSPVYASQAL--LGCPDRCGDLQIPYPFGTREGCYLNKNFL 60

Query: 61  ITCNKTHYDPPKAFLGDGNIDLTNISIIHGELRILQYVARDCYTQNGS--NIFNNPTINI 120
           ITCN TH+DPP+ FL  GNI +TNIS I GEL+IL + A+DCY +N S      + T+N+
Sbjct: 61  ITCNTTHFDPPRPFLRTGNIQVTNIS-ISGELQILHFGAKDCYPKNNSVDARRTSATLNL 120

Query: 121 SEFTISHTKNKFTVIGCDTYAFISGQLEGDSYRSGCMSLCGNSTTTIRDGSCSGNGCCQL 180
           S FT+S TKNKFTVIGCDTYAF+SGQ+EG SYR+ C++LC N  TT+RDG+CSGNGCCQL
Sbjct: 121 STFTVSSTKNKFTVIGCDTYAFLSGQIEGQSYRTACVALCDN-ITTVRDGACSGNGCCQL 180

Query: 181 EIPKGLKDLNLKVNSFENHTHVLNFNPCGYAFVIQQDNFTFSSTYIRNFTQEEVPMVLDW 240
           +IP GLK L  +V SF+NHT VL+FNPCGYAFV ++D F FS+ YIR+F Q +VP+VLDW
Sbjct: 181 DIPSGLKGLRYRVRSFDNHTDVLSFNPCGYAFVTEEDKFHFSAAYIRDFPQRQVPVVLDW 240

Query: 241 AIPTDTCSTADDKTNCICGPNSKKIDFLNDGSEYRCQCLDGFEGNPYLPQGCQDIDECKN 300
            I   TCSTA+ K+NC+CGPNS  ++ L DGSEYRC CLDGFEGNPYLP+GCQDIDEC++
Sbjct: 241 GISNTTCSTANKKSNCVCGPNSMMVNPLPDGSEYRCGCLDGFEGNPYLPRGCQDIDECRD 300

Query: 301 GS-HECKFIDKCVNTPGNYTCNCPKNYKGDGKRGGEGCTPNSKSFIQIIIGIGVGFIVLV 360
              ++CKF  +CVNT GNYTCNCP+ +KGDG+RGGEGCT +SKSF+Q+IIG+ VGF VLV
Sbjct: 301 ERLNDCKF--ECVNTEGNYTCNCPEGFKGDGRRGGEGCTRSSKSFVQVIIGVSVGFTVLV 360

Query: 361 IASTWLYLIYKKWKFIQRKEKFFEKNGGFMLQHHLSQRQSPADMLRIFTQEELEKATNKY 420
           I STWLYL Y+KWK I+ KEKFFE+NGG MLQ HLSQ +S  DM+ IFTQEEL+KATNKY
Sbjct: 361 IGSTWLYLGYRKWKLIKLKEKFFEENGGLMLQRHLSQWKSSTDMVTIFTQEELDKATNKY 420

Query: 421 DESAVVGRGGFGTVYKGVLDDGLTVAIKKSKMVDQSQTAQFINEVIVLSQINHRNVVKLL 480
           DESAV+G+GG+GTVYKG+L DG  VAIKKSK+VDQSQT+QFINEVIVLSQINHRNVVKLL
Sbjct: 421 DESAVIGKGGYGTVYKGILPDGSVVAIKKSKLVDQSQTSQFINEVIVLSQINHRNVVKLL 480

Query: 481 GCCLETQVPLLVYEFITNGTLFDHIHDKIKHVSLSWEARLKIASETAGVLSYLHSSASTP 540
           GCCLETQVPLLVYEF+TNGTLFDHIHD  KHV LSWEARL+IASETAGV+SYLHSSASTP
Sbjct: 481 GCCLETQVPLLVYEFVTNGTLFDHIHDTTKHVPLSWEARLRIASETAGVISYLHSSASTP 540

Query: 541 IIHRDIKSTNILLDDNCTAKVSDFGASKLVPLDHTQISTMVQGTLGYLDPEYLLTSELTE 600
           IIHRDIK+TNILLDDN  AKVSDFGASKLV LD TQ+STMVQGTLGYLDPEYLLTSELTE
Sbjct: 541 IIHRDIKTTNILLDDNYNAKVSDFGASKLVTLDQTQLSTMVQGTLGYLDPEYLLTSELTE 600

Query: 601 KSDVYSFGIVLLELITGKKAVCFAGPEAERNLAMYVLCAMKEDRLEEVVEKEMATEGNFE 660
           KSDVYSFGIVLLELITGKKAV F GPEAERNLAMYVLCAMKEDRL EVVEK MA EG F 
Sbjct: 601 KSDVYSFGIVLLELITGKKAVSFEGPEAERNLAMYVLCAMKEDRLGEVVEKGMAREGEFG 660

Query: 661 QIKEVAKVAKECVRVKGEERPSMKEVAMELEGLRLKQVEHLWAKNSLLGGALGTSQLVVS 720
           QIKEV KVA++C+R+ GEERPSMKEVAMELEGLR+  VEH W     L     ++  VVS
Sbjct: 661 QIKEVGKVARKCLRISGEERPSMKEVAMELEGLRV-MVEHEWVNEENLVADGASNMFVVS 720

Query: 721 GGMNAMDNSIKDQILSHIPHGR 736
           G  N +D+S+K QIL  I  GR
Sbjct: 721 GSTNVVDDSMKVQILPLIHDGR 735

BLAST of Sgr014327 vs. NCBI nr
Match: XP_022960687.1 (wall-associated receptor kinase 3-like [Cucurbita moschata])

HSP 1 Score: 1050.8 bits (2716), Expect = 5.4e-303
Identity = 527/741 (71.12%), Postives = 612/741 (82.59%), Query Frame = 0

Query: 3    RPTEKLVRLM----TLLSTALVATAATGSSDCERRCGNLEIPYPFGMKEGCYLNKNFSIT 62
            R  E L+RLM     +LS+ + A+ A     C  RCG+L+IPYPFG +EGCYLNKNF IT
Sbjct: 710  RQIETLIRLMIVNILILSSPVYASQAL--LGCPDRCGDLQIPYPFGTREGCYLNKNFLIT 769

Query: 63   CNKTHYDPPKAFLGDGNIDLTNISIIHGELRILQYVARDCYTQNGS--NIFNNPTINISE 122
            CN TH++PP+ FL  GNI +TNIS I GEL+IL + A+DCY +N S      + T+N+S 
Sbjct: 770  CNTTHFNPPRPFLRTGNIQVTNIS-ISGELQILHFGAKDCYPKNNSLDARRTSATLNLST 829

Query: 123  FTISHTKNKFTVIGCDTYAFISGQLEGDSYRSGCMSLCGNSTTTIRDGSCSGNGCCQLEI 182
            FT+S TKNKFTVIGCDTYAF+SGQ+EG SYR+ C++LC N  TT+RDG+CSGNGCCQL+I
Sbjct: 830  FTVSSTKNKFTVIGCDTYAFLSGQIEGQSYRTACVALCDN-ITTVRDGACSGNGCCQLDI 889

Query: 183  PKGLKDLNLKVNSFENHTHVLNFNPCGYAFVIQQDNFTFSSTYIRNFTQEEVPMVLDWAI 242
            P GLK L  +V SF+NHT VL+FNPCGYAFV ++D F FS+ YIR+F Q +VP+VLDW I
Sbjct: 890  PSGLKGLRYRVRSFDNHTDVLSFNPCGYAFVTEEDKFHFSAAYIRDFPQRQVPVVLDWGI 949

Query: 243  PTDTCSTADDKTNCICGPNSKKIDFLNDGSEYRCQCLDGFEGNPYLPQGCQDIDECKNGS 302
               TCSTA++K+NCICGPNS  ++ L+DGSEYRC+CLDGFEGNPYLP+GCQDIDEC++  
Sbjct: 950  SNTTCSTANNKSNCICGPNSMMVNPLHDGSEYRCRCLDGFEGNPYLPRGCQDIDECRDER 1009

Query: 303  -HECKFIDKCVNTPGNYTCNCPKNYKGDGKRGGEGCTPNSKSFIQIIIGIGVGFIVLVIA 362
             ++CKF  +CVNT GNYTCNCP+ +KGDG+RGGEGCT +SKSF+Q+IIG+ VGF VLVI 
Sbjct: 1010 LNDCKF--ECVNTKGNYTCNCPEGFKGDGRRGGEGCTRSSKSFVQVIIGVSVGFTVLVIG 1069

Query: 363  STWLYLIYKKWKFIQRKEKFFEKNGGFMLQHHLSQRQSPADMLRIFTQEELEKATNKYDE 422
            STWLYL Y+KWK I+ KEKFFE+NGG MLQ HLSQ +S  D + IFTQEEL+KATNKYDE
Sbjct: 1070 STWLYLGYRKWKLIKLKEKFFEENGGLMLQRHLSQWKSSTDTVTIFTQEELDKATNKYDE 1129

Query: 423  SAVVGRGGFGTVYKGVLDDGLTVAIKKSKMVDQSQTAQFINEVIVLSQINHRNVVKLLGC 482
            SAV+G+GG+GTVYKG L DG  VAIKKSK+VDQSQT+QFINEVIVLSQINHRNVVKLLGC
Sbjct: 1130 SAVIGKGGYGTVYKGFLPDGSVVAIKKSKLVDQSQTSQFINEVIVLSQINHRNVVKLLGC 1189

Query: 483  CLETQVPLLVYEFITNGTLFDHIHDKIKHVSLSWEARLKIASETAGVLSYLHSSASTPII 542
            CLETQVPLLVYEF+TNGTLFDHIHD  KHV LSWEARL+IASETAGV+SYLHSSASTPII
Sbjct: 1190 CLETQVPLLVYEFVTNGTLFDHIHDTTKHVPLSWEARLRIASETAGVISYLHSSASTPII 1249

Query: 543  HRDIKSTNILLDDNCTAKVSDFGASKLVPLDHTQISTMVQGTLGYLDPEYLLTSELTEKS 602
            HRDIK+TNILLDDN  AKVSDFGASKLVPLD TQ+STMVQGTLGYLDPEYLLTSELTEKS
Sbjct: 1250 HRDIKTTNILLDDNYNAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKS 1309

Query: 603  DVYSFGIVLLELITGKKAVCFAGPEAERNLAMYVLCAMKEDRLEEVVEKE-MATEGNFEQ 662
            DVYSFGIVLLELITGKKAV F GPEAERNLAMYVLCAMKEDRL +VVEK  MA E  FEQ
Sbjct: 1310 DVYSFGIVLLELITGKKAVSFEGPEAERNLAMYVLCAMKEDRLGDVVEKGIMAREREFEQ 1369

Query: 663  IKEVAKVAKECVRVKGEERPSMKEVAMELEGLRLKQVEHLWAKNSLLGGALGTSQLVVSG 722
            IKEV KVA++C+R+ GEERPSMKEVAMELEGLR+  VEH W     +     +   VVSG
Sbjct: 1370 IKEVGKVARKCLRISGEERPSMKEVAMELEGLRV-MVEHQWVNEENMVADGASHMFVVSG 1429

Query: 723  GMNAMDNSIKDQILSHIPHGR 736
              N +D+S+K Q+L  I  GR
Sbjct: 1430 STNVVDDSMKVQVLPLIHDGR 1443

BLAST of Sgr014327 vs. ExPASy Swiss-Prot
Match: Q9LMP1 (Wall-associated receptor kinase 2 OS=Arabidopsis thaliana OX=3702 GN=WAK2 PE=1 SV=1)

HSP 1 Score: 623.2 bits (1606), Expect = 3.6e-177
Identity = 326/680 (47.94%), Postives = 452/680 (66.47%), Query Frame = 0

Query: 29  DCERRCGNLEIPYPFGMKEGCYL--NKNFSITCNKTHYDPPKAFLGDGNIDLTNISIIHG 88
           +C+ RCGN+ + YPFG   GCY   +++F++TCN    +  K F   GN+ + N+S + G
Sbjct: 28  ECQTRCGNVAVEYPFGTSPGCYYPGDESFNLTCN----EQEKLFF--GNMPVINMS-LSG 87

Query: 89  ELRILQYVARDCYTQNGSNI-FNNPTINISEFTISHTKNKFTVIGCDTYAFISGQLEG-D 148
           +LR+    +R CY   G    +      +  FT+S   N+FTV+GC++YAF+  +  G +
Sbjct: 88  QLRVRLVRSRVCYDSQGKQTDYIAQRTTLGNFTLSEL-NRFTVVGCNSYAFL--RTSGVE 147

Query: 149 SYRSGCMSLCGNSTTTIRDGSCSGNGCCQLEIPKGLKDLNLKVNSFENHTHVLNFNPCGY 208
            Y +GC+S+C ++TT  ++GSCSG GCCQ+ +P+G   + +K +SF NH  V  FNPC Y
Sbjct: 148 KYSTGCISICDSATT--KNGSCSGEGCCQIPVPRGYSFVRVKPHSFHNHPTVHLFNPCTY 207

Query: 209 AFVIQQDNFTFSS----TYIRNFTQEEVPMVLDWAIPTDTCSTADDKTNCICGPNSKKID 268
           AF+++   F F +      +RN T    P+VLDW+I   TC   + +   +CG NS   D
Sbjct: 208 AFLVEDGMFDFHALEDLNNLRNVT--TFPVVLDWSIGDKTCKQVEYRG--VCGGNSTCFD 267

Query: 269 FLNDGSEYRCQCLDGFEGNPYLPQGCQDIDECKNGSHECKFIDKCVNTPGNYTCNCPKNY 328
               G+ Y C+CL+GFEGNPYLP GCQDI+EC +  H C     C NT G++ CNCP  Y
Sbjct: 268 -STGGTGYNCKCLEGFEGNPYLPNGCQDINECISSRHNCSEHSTCENTKGSFNCNCPSGY 327

Query: 329 KGDGKRGGEGCT----PNSKSFIQIIIGIGVGFIVLVIASTWLYLIYKKWKFIQRKEKFF 388
           + D       CT    P    + QI +G  +GF V+++  + L    K  K  + ++KFF
Sbjct: 328 RKDSL---NSCTRKVRPEYFRWTQIFLGTTIGFSVIMLGISCLQQKIKHRKNTELRQKFF 387

Query: 389 EKNGGFMLQHHLSQRQSPADMLRIFTQEELEKATNKYDESAVVGRGGFGTVYKGVLDDGL 448
           E+NGG ML   +S        ++IFT++ +++ATN Y ES ++G+GG GTVYKG+L D  
Sbjct: 388 EQNGGGMLIQRVSGAGPSNVDVKIFTEKGMKEATNGYHESRILGQGGQGTVYKGILPDNS 447

Query: 449 TVAIKKSKMVDQSQTAQFINEVIVLSQINHRNVVKLLGCCLETQVPLLVYEFITNGTLFD 508
            VAIKK+++ ++SQ  QFINEV+VLSQINHRNVVK+LGCCLET+VPLLVYEFI +GTLFD
Sbjct: 448 IVAIKKARLGNRSQVEQFINEVLVLSQINHRNVVKVLGCCLETEVPLLVYEFINSGTLFD 507

Query: 509 HIHDKIKHVSLSWEARLKIASETAGVLSYLHSSASTPIIHRDIKSTNILLDDNCTAKVSD 568
           H+H  +   SL+WE RL+IA+E AG L+YLHSSAS PIIHRDIK+ NILLD N TAKV+D
Sbjct: 508 HLHGSLYDSSLTWEHRLRIATEVAGSLAYLHSSASIPIIHRDIKTANILLDKNLTAKVAD 567

Query: 569 FGASKLVPLDHTQISTMVQGTLGYLDPEYLLTSELTEKSDVYSFGIVLLELITGKKAVCF 628
           FGAS+L+P+D  Q++T+VQGTLGYLDPEY  T  L EKSDVYSFG+VL+EL++G+KA+CF
Sbjct: 568 FGASRLIPMDKEQLTTIVQGTLGYLDPEYYNTGLLNEKSDVYSFGVVLMELLSGQKALCF 627

Query: 629 AGPEAERNLAMYVLCAMKEDRLEEVVEKEMATEGNFEQIKEVAKVAKECVRVKGEERPSM 688
             P   +NL      A K +R  E+++ ++  E N  +I+E A++A EC R+ GEERP M
Sbjct: 628 ERPHCPKNLVSCFASATKNNRFHEIIDGQVMNEDNQREIQEAARIAAECTRLMGEERPRM 687

Query: 689 KEVAMELEGLRLKQVEHLWA 697
           KEVA ELE LR+K  ++ W+
Sbjct: 688 KEVAAELEALRVKTTKYKWS 687

BLAST of Sgr014327 vs. ExPASy Swiss-Prot
Match: Q9LMN7 (Wall-associated receptor kinase 5 OS=Arabidopsis thaliana OX=3702 GN=WAK5 PE=2 SV=1)

HSP 1 Score: 596.3 bits (1536), Expect = 4.8e-169
Identity = 319/682 (46.77%), Postives = 441/682 (64.66%), Query Frame = 0

Query: 29  DCERRCGNLEIPYPFGMKEGCYL--NKNFSITCNKTHYDPPKAFLGDGNIDLTNISIIHG 88
           DC+ RCG++ I YPFG+  GCY   + +F+ITC +   D P       NI++ N +   G
Sbjct: 28  DCQTRCGDVPIDYPFGISTGCYYPGDDSFNITCEE---DKPNVL---SNIEVLNFN-HSG 87

Query: 89  ELRILQYVARDCYTQNGSNIFNNPTINISEFTISHTKNKFTVIGCDTYAFISGQLEGDSY 148
           +LR L   +  CY Q  +N F +    +   + S   NKFT++GC+ +A +S      +Y
Sbjct: 88  QLRGLIPRSTVCYDQQTNNDFESLWFRLDNLSFS-PNNKFTLVGCNAWALLS-TFGIQNY 147

Query: 149 RSGCMSLCGNSTTTIRDGSCSGNGCCQLE--IPKGLKDLNLKVNSFENHTHVLNFNPCGY 208
            +GCMSLC   T    +  C+G GCC+ E  IP     +  + + FEN T V +FNPC Y
Sbjct: 148 STGCMSLC--DTPPPPNSKCNGVGCCRTEVSIPLDSHRIETQPSRFENMTSVEHFNPCSY 207

Query: 209 AFVIQQDNFTFSSTY----IRNFTQEEVPMVLDWAIPTDTCSTADDKTNCICGPNSKKID 268
           AF ++   F FSS      +RN T+   P++LDW+I   TC     +   ICG NS   D
Sbjct: 208 AFFVEDGMFNFSSLEDLKDLRNVTR--FPVLLDWSIGNQTCEQVVGRN--ICGGNSTCFD 267

Query: 269 FLNDGSEYRCQCLDGFEGNPYLPQGCQDIDECKNGSHECKFIDKCVNTPGNYTCNCPKNY 328
               G  Y C+CL GF+GNPYL  GCQDI+EC    H C     C NT G++ C CP   
Sbjct: 268 STR-GKGYNCKCLQGFDGNPYLSDGCQDINECTTRIHNCSDTSTCENTLGSFHCQCPSG- 327

Query: 329 KGDGKRGGEGC------TPNSKSFIQIIIGIGVGFIVLVIASTWLYLIYKKWKFIQRKEK 388
             D       C       P    +  +++G  +GF+++++  +++    +  K  + +++
Sbjct: 328 -SDLNTTTMSCIDTPKEEPKYLGWTTVLLGTTIGFLIILLTISYIQQKMRHRKNTELRQQ 387

Query: 389 FFEKNGGFMLQHHLSQRQSPADMLRIFTQEELEKATNKYDESAVVGRGGFGTVYKGVLDD 448
           FFE+NGG ML   LS        ++IFT+E +++AT+ Y+ES ++G+GG GTVYKG+L D
Sbjct: 388 FFEQNGGGMLIQRLSGAGPSNVDVKIFTEEGMKEATDGYNESRILGQGGQGTVYKGILQD 447

Query: 449 GLTVAIKKSKMVDQSQTAQFINEVIVLSQINHRNVVKLLGCCLETQVPLLVYEFITNGTL 508
              VAIKK+++ D+SQ  QFINEV+VLSQINHRNVVKLLGCCLET+VPLLVYEFI++GTL
Sbjct: 448 NSIVAIKKARLGDRSQVEQFINEVLVLSQINHRNVVKLLGCCLETEVPLLVYEFISSGTL 507

Query: 509 FDHIHDKIKHVSLSWEARLKIASETAGVLSYLHSSASTPIIHRDIKSTNILLDDNCTAKV 568
           FDH+H  +   SL+WE RL+IA E AG L+YLHS AS PIIHRD+K+ NILLD+N TAKV
Sbjct: 508 FDHLHGSMFDSSLTWEHRLRIAIEVAGTLAYLHSYASIPIIHRDVKTANILLDENLTAKV 567

Query: 569 SDFGASKLVPLDHTQISTMVQGTLGYLDPEYLLTSELTEKSDVYSFGIVLLELITGKKAV 628
           +DFGAS+L+P+D  Q++TMVQGTLGYLDPEY  T  L EKSDVYSFG+VL+EL++G+KA+
Sbjct: 568 ADFGASRLIPMDQEQLTTMVQGTLGYLDPEYYNTGLLNEKSDVYSFGVVLMELLSGEKAL 627

Query: 629 CFAGPEAERNLAMYVLCAMKEDRLEEVVEKEMATEGNFEQIKEVAKVAKECVRVKGEERP 688
           CF  P++ ++L  Y + AMKE+RL E+++ ++  E N  +I+E A++A EC R+ GEERP
Sbjct: 628 CFERPQSSKHLVSYFVSAMKENRLHEIIDGQVMNEYNQREIQESARIAVECTRIMGEERP 687

Query: 689 SMKEVAMELEGLRLKQVEHLWA 697
           SMKEVA ELE LR+K  +H W+
Sbjct: 688 SMKEVAAELEALRVKTTKHQWS 691

BLAST of Sgr014327 vs. ExPASy Swiss-Prot
Match: Q39191 (Wall-associated receptor kinase 1 OS=Arabidopsis thaliana OX=3702 GN=WAK1 PE=1 SV=2)

HSP 1 Score: 584.3 bits (1505), Expect = 1.9e-165
Identity = 322/741 (43.45%), Postives = 457/741 (61.67%), Query Frame = 0

Query: 13  TLLSTALVATAATGSSDCERRCGNLEIPYPFGMKEGCYL--NKNFSITCNKTHYDPPKAF 72
           +L  T LV        +C+ +CGN+ I YPFG+  GCY   N++FSITC +   D P   
Sbjct: 15  SLACTQLVKGQHQPGENCQNKCGNITIEYPFGISSGCYYPGNESFSITCKE---DRPHVL 74

Query: 73  LGDGNIDLTNISIIHGELRILQYVARDCYTQNGSNIFNNPTINISEFTISHTKNKFTVIG 132
               +I++ N +   G+L++L   +  CY + G     + +  +   ++S   NK T +G
Sbjct: 75  ---SDIEVANFN-HSGQLQVLLNRSSTCYDEQGKKTEEDSSFTLENLSLS-ANNKLTAVG 134

Query: 133 CDTYAFISGQLEGDSYRSGCMSLCGNSTTTIRDGSCSGNGCCQLEIPKGLKDLNLKVNS- 192
           C+  + +       +Y + C+SLC +      DG C+G GCC++++   L     +  S 
Sbjct: 135 CNALSLLD-TFGMQNYSTACLSLCDSPPEA--DGECNGRGCCRVDVSAPLDSYTFETTSG 194

Query: 193 -FENHTHVLNFNPCGYAFVIQQDNFTFSST----YIRNFTQEEVPMVLDWAIPTDTCSTA 252
             ++ T   +F+PC YAF+++ D F FSST     +RN  +   P++LDW++   TC   
Sbjct: 195 RIKHMTSFHDFSPCTYAFLVEDDKFNFSSTEDLLNLRNVMR--FPVLLDWSVGNQTCEQV 254

Query: 253 DDKTNCICGPNSKKIDFLNDGSEYRCQCLDGFEGNPYLPQGCQDIDECKNGS----HECK 312
              +  ICG NS  +D     + Y C+C +GF+GNPYL  GCQD++EC   S    H C 
Sbjct: 255 G--STSICGGNSTCLD-STPRNGYICRCNEGFDGNPYLSAGCQDVNECTTSSTIHRHNCS 314

Query: 313 FIDKCVNTPGNYTCNCPKNYKGDGKRGGEGCTPNSKSFIQIIIGIGVGFIVLVIASTWLY 372
               C N  G + C C   Y+ D       C     ++  I++   +GF+V+++    + 
Sbjct: 315 DPKTCRNKVGGFYCKCQSGYRLDTTT--MSCKRKEFAWTTILLVTTIGFLVILLGVACIQ 374

Query: 373 LIYKKWKFIQRKEKFFEKNGGFMLQHHLSQRQSPADMLRIFTQEELEKATNKYDESAVVG 432
              K  K  + +E+FFE+NGG ML   LS        ++IFT++ ++KATN Y ES ++G
Sbjct: 375 QRMKHLKDTKLREQFFEQNGGGMLTQRLSGAGPSNVDVKIFTEDGMKKATNGYAESRILG 434

Query: 433 RGGFGTVYKGVLDDGLTVAIKKSKMVDQSQTAQFINEVIVLSQINHRNVVKLLGCCLETQ 492
           +GG GTVYKG+L D   VAIKK+++ D SQ  QFINEV+VLSQINHRNVVKLLGCCLET+
Sbjct: 435 QGGQGTVYKGILPDNSIVAIKKARLGDSSQVEQFINEVLVLSQINHRNVVKLLGCCLETE 494

Query: 493 VPLLVYEFITNGTLFDHIHDKIKHVSLSWEARLKIASETAGVLSYLHSSASTPIIHRDIK 552
           VPLLVYEFITNGTLFDH+H  +   SL+WE RLKIA E AG L+YLHSSAS PIIHRDIK
Sbjct: 495 VPLLVYEFITNGTLFDHLHGSMIDSSLTWEHRLKIAIEVAGTLAYLHSSASIPIIHRDIK 554

Query: 553 STNILLDDNCTAKVSDFGASKLVPLDHTQISTMVQGTLGYLDPEYLLTSELTEKSDVYSF 612
           + NILLD N TAKV+DFGAS+L+P+D  ++ TMVQGTLGYLDPEY  T  L EKSDVYSF
Sbjct: 555 TANILLDVNLTAKVADFGASRLIPMDKEELETMVQGTLGYLDPEYYNTGLLNEKSDVYSF 614

Query: 613 GIVLLELITGKKAVCFAGPEAERNLAMYVLCAMKEDRLEEVVEKEMATEGNFEQIKEVAK 672
           G+VL+EL++G+KA+CF  P++ ++L  Y   A KE+RL+E++  E+  E N ++I+E A+
Sbjct: 615 GVVLMELLSGQKALCFKRPQSSKHLVSYFATATKENRLDEIIGGEVMNEDNLKEIQEAAR 674

Query: 673 VAKECVRVKGEERPSMKEVAMELEGLRLKQVEHLWA------KNSLLGGALGTSQLVVSG 732
           +A EC R+ GEERP MKEVA +LE LR+++ +H W+         L+GG + ++Q   S 
Sbjct: 675 IAAECTRLMGEERPRMKEVAAKLEALRVEKTKHKWSDQYPEENEHLIGGHILSAQGETSS 734

Query: 733 GMNAMDNSIKDQILSHIPHGR 736
            +    +SIK+  +  I  GR
Sbjct: 735 SIGY--DSIKNVAILDIETGR 735

BLAST of Sgr014327 vs. ExPASy Swiss-Prot
Match: Q9LMN8 (Wall-associated receptor kinase 3 OS=Arabidopsis thaliana OX=3702 GN=WAK3 PE=2 SV=2)

HSP 1 Score: 581.6 bits (1498), Expect = 1.2e-164
Identity = 334/755 (44.24%), Postives = 463/755 (61.32%), Query Frame = 0

Query: 8   LVRLMTLLSTALVATAATGSSDCERRCGNLEIPYPFGMKEGCYL--NKNFSITCNKTHYD 67
           LV +  L  T LV        DC+ +CGN+ I YPFG+  GCY   + NF++TC      
Sbjct: 9   LVVIFFLAYTQLVKGQHQPREDCKLKCGNVTIEYPFGISTGCYYPGDDNFNLTC-----V 68

Query: 68  PPKAFLGDGNIDLTNISIIHGELRILQYVARDCYTQNGSNIFNNPTINI---SEFTISHT 127
             +  L  G I +TNIS   G + +L     +CY Q   N  N   +     S F++S +
Sbjct: 69  VEEKLLLFGIIQVTNIS-HSGHVSVLFERFSECYEQ--KNETNGTALGYQLGSSFSLS-S 128

Query: 128 KNKFTVIGCDTYAFISGQLEGDSYRSGCMSLCGNSTTTIRDGSCSGNGCCQLE------- 187
            NKFT++GC+  + +S      +Y +GC+SLC +      +G C+G GCC  E       
Sbjct: 129 NNKFTLVGCNALSLLS-TFGKQNYSTGCLSLCNSQPEA--NGRCNGVGCCTTEDFSVPFD 188

Query: 188 ---IPKGLKDLNLKVNSFEN--HTHVLNFNPCGYAFVIQQDNFTFSST----YIRNFTQE 247
                 G   L  +VN+  +  +T V  FNPC YAF+++   F F S+     +RN T+ 
Sbjct: 189 SDTFQFGSVRLRNQVNNSLDLFNTSVYQFNPCTYAFLVEDGKFNFDSSKDLKNLRNVTR- 248

Query: 248 EVPMVLDWAIPTDTCSTADDKTNCICGPNSKKIDFLNDGSEYRCQCLDGFEGNPYLPQGC 307
             P+ LDW+I   TC  A   +  ICG NS   +       Y C+C +G++GNPY  +GC
Sbjct: 249 -FPVALDWSIGNQTCEQAG--STRICGKNSSCYNSTTRNG-YICKCNEGYDGNPYRSEGC 308

Query: 308 QDIDECKNGSHECKFIDKCVNTPGNYTCNCPKNYKGDGKRGGEGCTPNSKSFIQIIIGIG 367
           +DIDEC + +H C     C N  G + C CP  Y          CT       +I + I 
Sbjct: 309 KDIDECISDTHNCSDPKTCRNRDGGFDCKCPSGY---DLNSSMSCTRPEYKRTRIFLVII 368

Query: 368 VGFIVLVIASTWLYLIYKKWKFIQRKEKFFEKNGGFMLQHHLSQRQSPADMLRIFTQEEL 427
           +G +VL++A+  +    K+ K+ + + +FFE+NGG ML   LS         +IFT+E +
Sbjct: 369 IGVLVLLLAAICIQHATKQRKYTKLRRQFFEQNGGGMLIQRLSGAGLSNIDFKIFTEEGM 428

Query: 428 EKATNKYDESAVVGRGGFGTVYKGVLDDGLTVAIKKSKMVDQSQTAQFINEVIVLSQINH 487
           ++ATN YDES ++G+GG GTVYKG+L D   VAIKK+++ D  Q  QFI+EV+VLSQINH
Sbjct: 429 KEATNGYDESRILGQGGQGTVYKGILPDNTIVAIKKARLADSRQVDQFIHEVLVLSQINH 488

Query: 488 RNVVKLLGCCLETQVPLLVYEFITNGTLFDHIHDKIKHVSLSWEARLKIASETAGVLSYL 547
           RNVVK+LGCCLET+VPLLVYEFITNGTLFDH+H  I   SL+WE RL+IA E AG L+YL
Sbjct: 489 RNVVKILGCCLETEVPLLVYEFITNGTLFDHLHGSIFDSSLTWEHRLRIAIEVAGTLAYL 548

Query: 548 HSSASTPIIHRDIKSTNILLDDNCTAKVSDFGASKLVPLDHTQISTMVQGTLGYLDPEYL 607
           HSSAS PIIHRDIK+ NILLD+N TAKV+DFGASKL+P+D  Q++TMVQGTLGYLDPEY 
Sbjct: 549 HSSASIPIIHRDIKTANILLDENLTAKVADFGASKLIPMDKEQLTTMVQGTLGYLDPEYY 608

Query: 608 LTSELTEKSDVYSFGIVLLELITGKKAVCFAGPEAERNLAMYVLCAMKEDRLEEVVEKEM 667
            T  L EKSDVYSFG+VL+EL++G+KA+CF  P+A ++L  Y + A +E+RL E+++ ++
Sbjct: 609 TTGLLNEKSDVYSFGVVLMELLSGQKALCFERPQASKHLVSYFVSATEENRLHEIIDDQV 668

Query: 668 ATEGNFEQIKEVAKVAKECVRVKGEERPSMKEVAMELEGLRLKQVEHLWA------KNSL 727
             E N ++I+E A++A EC R+ GEERP MKEVA +LE LR+++ +H W+         L
Sbjct: 669 LNEDNLKEIQEAARIAAECTRLMGEERPRMKEVAAKLEALRVEKTKHKWSDQYPEENEHL 728

Query: 728 LGGALGTSQLVVSGGMNAMDNSIKDQILSHIPHGR 736
           +GG + ++Q   S  +    +SIK+  +  I  GR
Sbjct: 729 IGGHILSAQGETSSSIGY--DSIKNVAILDIETGR 741

BLAST of Sgr014327 vs. ExPASy Swiss-Prot
Match: Q9LMN6 (Wall-associated receptor kinase 4 OS=Arabidopsis thaliana OX=3702 GN=WAK4 PE=2 SV=1)

HSP 1 Score: 547.7 bits (1410), Expect = 1.9e-154
Identity = 299/689 (43.40%), Postives = 433/689 (62.84%), Query Frame = 0

Query: 30  CERRCGNLEIPYPFGMKEGCYL--NKNFSITCNKTHYDPPKAFLGDGNIDLTNISIIHGE 89
           C  +CGN+ + YPFG   GC+   + +F+++C   +      + G   +++++ S    +
Sbjct: 28  CPEKCGNVTLEYPFGFSPGCWRAEDPSFNLSCVNENL----FYKGLEVVEISHSS----Q 87

Query: 90  LRILQYVARDCYTQNGSNIFNNPTI---NISEFTISHTKNKFTVIGCDTYAFISGQLEGD 149
           LR+L   +  CY   G   F   T    N+   T+S   N  T +GC++YAF+S    G 
Sbjct: 88  LRVLYPASYICYNSKGK--FAKGTYYWSNLGNLTLS-GNNTITALGCNSYAFVSS--NGT 147

Query: 150 SYRS-GCMSLCGNSTTTIRDGSCSGNGCCQLEIPKGLKDLNLKVNSFENHTHV--LNFNP 209
              S GC+S C ++ +   +G C+G GCCQ  +P G   L ++   F+N T V  ++   
Sbjct: 148 RRNSVGCISAC-DALSHEANGECNGEGCCQNPVPAGNNWLIVRSYRFDNDTSVQPISEGQ 207

Query: 210 CGYAFVIQQDNFTFSSTYIRNFTQEE---VPMVLDWAIPTDTCSTADDK---TNCICGPN 269
           C YAF+++   F ++++   ++ Q      P+VLDW+I  +TC    +K    N IC  +
Sbjct: 208 CIYAFLVENGKFKYNASDKYSYLQNRNVGFPVVLDWSIRGETCGQVGEKKCGVNGICSNS 267

Query: 270 SKKIDFLNDGSEYRCQCLDGFEGNPYLPQGCQDIDECKNGS----HECKFIDKCVNTPGN 329
           +        G  Y C+C  GF+GNPYL  GCQDI+EC   +    H C     C N  G+
Sbjct: 268 A-------SGIGYTCKCKGGFQGNPYLQNGCQDINECTTANPIHKHNCSGDSTCENKLGH 327

Query: 330 YTCNCPKNYKGDGKRGGEGCTPNSK----SFIQIIIGIGVGFIVLVIASTWLYLIYKKWK 389
           + CNC   Y+ +       C P        +  I++G  +GF+V+++A + +    K  K
Sbjct: 328 FRCNCRSRYELNTTT--NTCKPKGNPEYVEWTTIVLGTTIGFLVILLAISCIEHKMKNTK 387

Query: 390 FIQRKEKFFEKNGGFMLQHHLSQRQSPADMLRIFTQEELEKATNKYDESAVVGRGGFGTV 449
             + +++FFE+NGG ML   LS        ++IFT+E +++AT+ YDE+ ++G+GG GTV
Sbjct: 388 DTELRQQFFEQNGGGMLMQRLSGAGPSNVDVKIFTEEGMKEATDGYDENRILGQGGQGTV 447

Query: 450 YKGVLDDGLTVAIKKSKMVDQSQTAQFINEVIVLSQINHRNVVKLLGCCLETQVPLLVYE 509
           YKG+L D   VAIKK+++ D SQ  QFINEV+VLSQINHRNVVKLLGCCLET+VPLLVYE
Sbjct: 448 YKGILPDNSIVAIKKARLGDNSQVEQFINEVLVLSQINHRNVVKLLGCCLETEVPLLVYE 507

Query: 510 FITNGTLFDHIHDKIKHVSLSWEARLKIASETAGVLSYLHSSASTPIIHRDIKSTNILLD 569
           FI++GTLFDH+H  +   SL+WE RL++A E AG L+YLHSSAS PIIHRDIK+ NILLD
Sbjct: 508 FISSGTLFDHLHGSMFDSSLTWEHRLRMAVEIAGTLAYLHSSASIPIIHRDIKTANILLD 567

Query: 570 DNCTAKVSDFGASKLVPLDHTQISTMVQGTLGYLDPEYLLTSELTEKSDVYSFGIVLLEL 629
           +N TAKV+DFGAS+L+P+D   ++TMVQGTLGYLDPEY  T  L EKSDVYSFG+VL+EL
Sbjct: 568 ENLTAKVADFGASRLIPMDKEDLATMVQGTLGYLDPEYYNTGLLNEKSDVYSFGVVLMEL 627

Query: 630 ITGKKAVCFAGPEAERNLAMYVLCAMKEDRLEEVVEKEMATEGNFEQIKEVAKVAKECVR 689
           ++G+KA+CF  P+  +++  Y   A KE+RL E+++ ++  E N  +I++ A++A EC R
Sbjct: 628 LSGQKALCFERPQTSKHIVSYFASATKENRLHEIIDGQVMNENNQREIQKAARIAVECTR 687

Query: 690 VKGEERPSMKEVAMELEGLRLKQVEHLWA 697
           + GEERP MKEVA ELE LR+ + +H W+
Sbjct: 688 LTGEERPGMKEVAAELEALRVTKTKHKWS 693

BLAST of Sgr014327 vs. ExPASy TrEMBL
Match: A0A6J1JNA5 (wall-associated receptor kinase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111486858 PE=4 SV=1)

HSP 1 Score: 1059.7 bits (2739), Expect = 5.6e-306
Identity = 526/742 (70.89%), Postives = 611/742 (82.35%), Query Frame = 0

Query: 1   MGRPTEKLVRLM----TLLSTALVATAATGSSDCERRCGNLEIPYPFGMKEGCYLNKNFS 60
           MGR  +  +RLM     +LS ++ A A+     C+ RCG+L+IPYPFG +EGCYLNKNF 
Sbjct: 1   MGRQIKTHIRLMIINILILSPSVYAVASQALLGCDDRCGDLQIPYPFGTREGCYLNKNFL 60

Query: 61  ITCNKTHYDPPKAFLGDGNIDLTNISIIHGELRILQYVARDCYTQNGS--NIFNNPTINI 120
           ITCN TH+DPP+ FL  GNI +TNIS I GEL+IL + A+DCY +N S      + T+N+
Sbjct: 61  ITCNTTHFDPPRPFLRTGNIQVTNIS-ISGELQILHFGAKDCYPKNNSLDARRTSATLNL 120

Query: 121 SEFTISHTKNKFTVIGCDTYAFISGQLEGDSYRSGCMSLCGNSTTTIRDGSCSGNGCCQL 180
           S FT+S TKNKFTVIGCDTYAF+SGQ+EG SYR+ C++LC N  TT+RDG+CSGNGCCQL
Sbjct: 121 STFTVSSTKNKFTVIGCDTYAFLSGQIEGQSYRTACVALCDN-ITTVRDGACSGNGCCQL 180

Query: 181 EIPKGLKDLNLKVNSFENHTHVLNFNPCGYAFVIQQDNFTFSSTYIRNFTQEEVPMVLDW 240
           +IP GLK L  +V SF+NHT V +FNPCGYAFV ++D F FS+ YIRNF Q +VP+VLDW
Sbjct: 181 DIPSGLKGLRYRVRSFDNHTDVFSFNPCGYAFVTEEDKFHFSAAYIRNFPQRQVPVVLDW 240

Query: 241 AIPTDTCSTADDKTNCICGPNSKKIDFLNDGSEYRCQCLDGFEGNPYLPQGCQDIDECKN 300
            I   TCSTA++K+NCICGPNS  ++ L DGSEYRC CLDGFEGNPYLP+GCQDIDEC++
Sbjct: 241 GISNTTCSTANNKSNCICGPNSMMVNVLPDGSEYRCGCLDGFEGNPYLPRGCQDIDECRD 300

Query: 301 GS-HECKFIDKCVNTPGNYTCNCPKNYKGDGKRGGEGCTPNSKSFIQIIIGIGVGFIVLV 360
              ++CKF  +CVNT GNYTCNCP+ +KGDG+RGGEGCT +SKSF+Q+IIG+ VGF VLV
Sbjct: 301 ERLNDCKF--ECVNTKGNYTCNCPEGFKGDGRRGGEGCTRSSKSFVQVIIGVSVGFTVLV 360

Query: 361 IASTWLYLIYKKWKFIQRKEKFFEKNGGFMLQHHLSQRQSPADMLRIFTQEELEKATNKY 420
           I STWLYL Y+KWK I+ KEKFFE+NGG MLQ HLSQ +S  D + IFTQEEL+KATNKY
Sbjct: 361 IGSTWLYLGYRKWKLIKLKEKFFEENGGLMLQRHLSQWKSSTDTVTIFTQEELDKATNKY 420

Query: 421 DESAVVGRGGFGTVYKGVLDDGLTVAIKKSKMVDQSQTAQFINEVIVLSQINHRNVVKLL 480
           DESAV+G+GG+GTVYKG+L DG  VAIKKSK+VDQSQT+QFINEVIVLSQINHRNVVKLL
Sbjct: 421 DESAVIGKGGYGTVYKGILPDGSVVAIKKSKLVDQSQTSQFINEVIVLSQINHRNVVKLL 480

Query: 481 GCCLETQVPLLVYEFITNGTLFDHIHDKIKHVSLSWEARLKIASETAGVLSYLHSSASTP 540
           GCCLETQVPLLVYEF+TNGTLFDHIHD  KHV LSW+ARL+IA ETAGV+SYLHSSASTP
Sbjct: 481 GCCLETQVPLLVYEFVTNGTLFDHIHDTTKHVPLSWKARLRIALETAGVISYLHSSASTP 540

Query: 541 IIHRDIKSTNILLDDNCTAKVSDFGASKLVPLDHTQISTMVQGTLGYLDPEYLLTSELTE 600
           IIHRDIK+TNILLDDN  AKVSDFGASKLVPLD TQ+STMVQGTLGYLDPEYLLTSELTE
Sbjct: 541 IIHRDIKTTNILLDDNYNAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTE 600

Query: 601 KSDVYSFGIVLLELITGKKAVCFAGPEAERNLAMYVLCAMKEDRLEEVVEKEMATEGNFE 660
           KSDVYSFGIVLLELITGKKAV F GPEAERNLAMYVLCAMKEDRL EVVEK MA EG FE
Sbjct: 601 KSDVYSFGIVLLELITGKKAVSFEGPEAERNLAMYVLCAMKEDRLGEVVEKGMAREGEFE 660

Query: 661 QIKEVAKVAKECVRVKGEERPSMKEVAMELEGLRLKQVEHLWAKNSLLGGALGTSQLVVS 720
           QIK+V KVA++C+R+ GEERPSMKEV MELEGLR+   EH W     L     ++  VVS
Sbjct: 661 QIKDVGKVARKCLRISGEERPSMKEVVMELEGLRV-MAEHEWVDEENLVADGASNIFVVS 720

Query: 721 GGMNAMDNSIKDQILSHIPHGR 736
           G  N +D+S+K Q+L  I  GR
Sbjct: 721 GSTNVVDDSMKVQVLPLIHDGR 737

BLAST of Sgr014327 vs. ExPASy TrEMBL
Match: A0A6J1H843 (wall-associated receptor kinase 3-like OS=Cucurbita moschata OX=3662 GN=LOC111461403 PE=4 SV=1)

HSP 1 Score: 1050.8 bits (2716), Expect = 2.6e-303
Identity = 527/741 (71.12%), Postives = 612/741 (82.59%), Query Frame = 0

Query: 3    RPTEKLVRLM----TLLSTALVATAATGSSDCERRCGNLEIPYPFGMKEGCYLNKNFSIT 62
            R  E L+RLM     +LS+ + A+ A     C  RCG+L+IPYPFG +EGCYLNKNF IT
Sbjct: 710  RQIETLIRLMIVNILILSSPVYASQAL--LGCPDRCGDLQIPYPFGTREGCYLNKNFLIT 769

Query: 63   CNKTHYDPPKAFLGDGNIDLTNISIIHGELRILQYVARDCYTQNGS--NIFNNPTINISE 122
            CN TH++PP+ FL  GNI +TNIS I GEL+IL + A+DCY +N S      + T+N+S 
Sbjct: 770  CNTTHFNPPRPFLRTGNIQVTNIS-ISGELQILHFGAKDCYPKNNSLDARRTSATLNLST 829

Query: 123  FTISHTKNKFTVIGCDTYAFISGQLEGDSYRSGCMSLCGNSTTTIRDGSCSGNGCCQLEI 182
            FT+S TKNKFTVIGCDTYAF+SGQ+EG SYR+ C++LC N  TT+RDG+CSGNGCCQL+I
Sbjct: 830  FTVSSTKNKFTVIGCDTYAFLSGQIEGQSYRTACVALCDN-ITTVRDGACSGNGCCQLDI 889

Query: 183  PKGLKDLNLKVNSFENHTHVLNFNPCGYAFVIQQDNFTFSSTYIRNFTQEEVPMVLDWAI 242
            P GLK L  +V SF+NHT VL+FNPCGYAFV ++D F FS+ YIR+F Q +VP+VLDW I
Sbjct: 890  PSGLKGLRYRVRSFDNHTDVLSFNPCGYAFVTEEDKFHFSAAYIRDFPQRQVPVVLDWGI 949

Query: 243  PTDTCSTADDKTNCICGPNSKKIDFLNDGSEYRCQCLDGFEGNPYLPQGCQDIDECKNGS 302
               TCSTA++K+NCICGPNS  ++ L+DGSEYRC+CLDGFEGNPYLP+GCQDIDEC++  
Sbjct: 950  SNTTCSTANNKSNCICGPNSMMVNPLHDGSEYRCRCLDGFEGNPYLPRGCQDIDECRDER 1009

Query: 303  -HECKFIDKCVNTPGNYTCNCPKNYKGDGKRGGEGCTPNSKSFIQIIIGIGVGFIVLVIA 362
             ++CKF  +CVNT GNYTCNCP+ +KGDG+RGGEGCT +SKSF+Q+IIG+ VGF VLVI 
Sbjct: 1010 LNDCKF--ECVNTKGNYTCNCPEGFKGDGRRGGEGCTRSSKSFVQVIIGVSVGFTVLVIG 1069

Query: 363  STWLYLIYKKWKFIQRKEKFFEKNGGFMLQHHLSQRQSPADMLRIFTQEELEKATNKYDE 422
            STWLYL Y+KWK I+ KEKFFE+NGG MLQ HLSQ +S  D + IFTQEEL+KATNKYDE
Sbjct: 1070 STWLYLGYRKWKLIKLKEKFFEENGGLMLQRHLSQWKSSTDTVTIFTQEELDKATNKYDE 1129

Query: 423  SAVVGRGGFGTVYKGVLDDGLTVAIKKSKMVDQSQTAQFINEVIVLSQINHRNVVKLLGC 482
            SAV+G+GG+GTVYKG L DG  VAIKKSK+VDQSQT+QFINEVIVLSQINHRNVVKLLGC
Sbjct: 1130 SAVIGKGGYGTVYKGFLPDGSVVAIKKSKLVDQSQTSQFINEVIVLSQINHRNVVKLLGC 1189

Query: 483  CLETQVPLLVYEFITNGTLFDHIHDKIKHVSLSWEARLKIASETAGVLSYLHSSASTPII 542
            CLETQVPLLVYEF+TNGTLFDHIHD  KHV LSWEARL+IASETAGV+SYLHSSASTPII
Sbjct: 1190 CLETQVPLLVYEFVTNGTLFDHIHDTTKHVPLSWEARLRIASETAGVISYLHSSASTPII 1249

Query: 543  HRDIKSTNILLDDNCTAKVSDFGASKLVPLDHTQISTMVQGTLGYLDPEYLLTSELTEKS 602
            HRDIK+TNILLDDN  AKVSDFGASKLVPLD TQ+STMVQGTLGYLDPEYLLTSELTEKS
Sbjct: 1250 HRDIKTTNILLDDNYNAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKS 1309

Query: 603  DVYSFGIVLLELITGKKAVCFAGPEAERNLAMYVLCAMKEDRLEEVVEKE-MATEGNFEQ 662
            DVYSFGIVLLELITGKKAV F GPEAERNLAMYVLCAMKEDRL +VVEK  MA E  FEQ
Sbjct: 1310 DVYSFGIVLLELITGKKAVSFEGPEAERNLAMYVLCAMKEDRLGDVVEKGIMAREREFEQ 1369

Query: 663  IKEVAKVAKECVRVKGEERPSMKEVAMELEGLRLKQVEHLWAKNSLLGGALGTSQLVVSG 722
            IKEV KVA++C+R+ GEERPSMKEVAMELEGLR+  VEH W     +     +   VVSG
Sbjct: 1370 IKEVGKVARKCLRISGEERPSMKEVAMELEGLRV-MVEHQWVNEENMVADGASHMFVVSG 1429

Query: 723  GMNAMDNSIKDQILSHIPHGR 736
              N +D+S+K Q+L  I  GR
Sbjct: 1430 STNVVDDSMKVQVLPLIHDGR 1443

BLAST of Sgr014327 vs. ExPASy TrEMBL
Match: A0A6J1CJM0 (putative wall-associated receptor kinase-like 16 OS=Momordica charantia OX=3673 GN=LOC111011910 PE=4 SV=1)

HSP 1 Score: 1041.6 bits (2692), Expect = 1.6e-300
Identity = 529/752 (70.35%), Postives = 616/752 (81.91%), Query Frame = 0

Query: 6   EKLVRLMTL-----LSTALVATAATGSSD----CERRCGNLEIPYPFGMKEGCYLNKNFS 65
           E L+RL+       + +A  ATAA G+S     CE  CG+++IPYPFGMKEGCYLN+ F 
Sbjct: 2   ETLMRLIITAVQIGILSAAAATAAAGASQALPGCEEWCGDVQIPYPFGMKEGCYLNEKFF 61

Query: 66  ITCNKTHYDPPKAFLGDGNIDLTNISIIHGELRILQYVARDCYTQNGSNIFNNPTINISE 125
           I+CNKTH D PKAFL  GN+++TNIS I GEL IL + ARDCY  N     +   +N +E
Sbjct: 62  ISCNKTHNDSPKAFLQGGNLNVTNIS-ISGELNILHFWARDCYPPNTLPDLSTAFLN-TE 121

Query: 126 FTISHTKNKFTVIGCDTYAFISGQLEGDS-YRSGCMSLCGNSTTTIRDGSCSGNGCCQLE 185
           F +S  KNKFTVIGCDT++FISG + G   Y+S C++LC +  TT++DG+CSGNGCCQLE
Sbjct: 122 FAVSSAKNKFTVIGCDTFSFISGLVGGTQFYKSACVALC-DDITTVKDGACSGNGCCQLE 181

Query: 186 IPKGLKDLNLKVNSFENHTHVLNFNPCGYAFVIQQDNFTFSSTYIRNFTQEEVPMVLDWA 245
           IPKGL +LN  V+SF+NHT+VL+FNPCGYAFVI++D F FSS YIR+F  E VP+VLDWA
Sbjct: 182 IPKGLNNLNYTVSSFDNHTNVLSFNPCGYAFVIEEDKFNFSSKYIRHFPLERVPLVLDWA 241

Query: 246 IPTDTCSTADDKTNCICGPNSKKIDFLNDGSEYRCQCLDGFEGNPYLPQGCQDIDECKNG 305
           I  +TC   ++ TNCICGP+S+K++F+ DGSEYRCQC DGFEGNPYLP+GCQD+DECK+G
Sbjct: 242 ISNNTC---ENTTNCICGPSSRKVNFVEDGSEYRCQCSDGFEGNPYLPRGCQDVDECKDG 301

Query: 306 SHECKFIDKCVNTPGNYTCNCPKNYKGDGKRGGEGCTPNSKSFIQIIIGIGVGFIVLVIA 365
            H+CKF  +CVNT GNYTCNCP+ ++GDG+R GEGCT NSKSF+QII+G+ VGF VL+I 
Sbjct: 302 RHDCKF--ECVNTEGNYTCNCPEGFRGDGRREGEGCTRNSKSFVQIIVGVTVGFTVLLIG 361

Query: 366 STWLYLIYKKWKFIQRKEKFFEKNGGFMLQHHLSQRQSPADMLRIFTQEELEKATNKYDE 425
            TW YL Y+KWKF++ KE+FFEKNGG MLQ HLSQ Q+ ADM+RIFTQEELEKATNKYDE
Sbjct: 362 LTWFYLGYRKWKFVKLKEEFFEKNGGLMLQQHLSQWQTSADMVRIFTQEELEKATNKYDE 421

Query: 426 SAVVGRGGFGTVYKGVLDDGLTVAIKKSKMVDQSQTAQFINEVIVLSQINHRNVVKLLGC 485
           SAVVG+GG+GTVYKGVL+DGL VAIKKSK+VDQSQT+QFINEV+VLSQINHRNVVKLLGC
Sbjct: 422 SAVVGKGGYGTVYKGVLEDGLVVAIKKSKLVDQSQTSQFINEVMVLSQINHRNVVKLLGC 481

Query: 486 CLETQVPLLVYEFITNGTLFDHIHDKIKH-VSLSWEARLKIASETAGVLSYLHSSASTPI 545
           CLETQVPLLVYEFITNGTL+DHIHDK  H  SL WEARL+IASETAGV+SYLHSSASTPI
Sbjct: 482 CLETQVPLLVYEFITNGTLYDHIHDKANHDYSLPWEARLRIASETAGVISYLHSSASTPI 541

Query: 546 IHRDIKSTNILLDDNCTAKVSDFGASKLVPLDHTQISTMVQGTLGYLDPEYLLTSELTEK 605
           IHRDIK+TNILLD N TAKVSDFGASKLVPLD TQ+STMVQGTLGYLDPEYLLTSELTEK
Sbjct: 542 IHRDIKTTNILLDQNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEK 601

Query: 606 SDVYSFGIVLLELITGKKAVCFAGPEAERNLAMYVLCAMKEDRLEEVVEKEMATEGNFEQ 665
           SDVYSFGIVL+ELITGKKAV F GPEAERNLAMYVLCAMKEDRLEEVVEK MA EG  EQ
Sbjct: 602 SDVYSFGIVLMELITGKKAVSFEGPEAERNLAMYVLCAMKEDRLEEVVEKGMAREGKLEQ 661

Query: 666 IKEVAKVAKECVRVKGEERPSMKEVAMELEGLRLKQVEHLWAKNS-----------LLGG 725
           IKEVAKVAKEC+RV+GEERPSMKEVAMELEGLR+  VE+ W  ++           LL  
Sbjct: 662 IKEVAKVAKECLRVRGEERPSMKEVAMELEGLRV-LVENKWVNDNNLINAEEMVAYLLED 721

Query: 726 ALGTSQLVVSGGMNAMDNSIKDQILSHIPHGR 736
             G S+  +    N +D+S+K QIL  I  GR
Sbjct: 722 NNGASESTLLSMNNVVDDSMKVQILPRIHDGR 744

BLAST of Sgr014327 vs. ExPASy TrEMBL
Match: A0A5D3DKI3 (Wall-associated receptor kinase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1607G001160 PE=4 SV=1)

HSP 1 Score: 1040.4 bits (2689), Expect = 3.5e-300
Identity = 526/742 (70.89%), Postives = 610/742 (82.21%), Query Frame = 0

Query: 6   EKLVRLMTLLSTALVATAATGS--SDCERRCGNLEIPYPFGMKEGCYLNKNFSITCNKTH 65
           E L++L+ LLS  L A        + C+  CGNL IPYPFG+K+ CYL++ FSITCNKT 
Sbjct: 6   EDLMKLIILLSGTLAAAVEVAKVLTGCDEHCGNLRIPYPFGIKKRCYLDQAFSITCNKT- 65

Query: 66  YDPPKAFLGDGNIDLTNISIIHGELRILQYVARDCYTQNGSNIFN-NPTINISEFTISHT 125
           Y PPK FL D NI++TNISII G+L I Q+VARDCYT+NG +  N  P + +++F IS+T
Sbjct: 66  YHPPKPFLQDSNIEITNISIIQGQLHIKQFVARDCYTKNGPSESNTRPFLLLNKFRISNT 125

Query: 126 KNKFTVIGCDTYAFISGQLEGDSYRSGCMSLCGNSTT-TIRDGSCSGNGCCQLEIPKGLK 185
            NKF VIGCDTYA+I G++EG+SY+SGCM+LCGN+ T  I+DGSCSGNGCCQL+IPKGLK
Sbjct: 126 DNKFIVIGCDTYAYIYGEIEGESYKSGCMALCGNNNTKIIKDGSCSGNGCCQLQIPKGLK 185

Query: 186 DLNLKVNSFENHTHVLNFNPCGYAFVIQQDNFTFSSTYIRNFTQEEVPMVLDWAIPTDT- 245
            L L+V SF+NH+ VL FNPCGYAFVIQQD FTFS  YI NFTQEEVP+VLDW IPT+T 
Sbjct: 186 ILELEVRSFDNHSEVLKFNPCGYAFVIQQDKFTFSKKYIYNFTQEEVPLVLDWGIPTNTS 245

Query: 246 CSTADDKTNC-ICGPNSKKIDFLNDGSEYRCQCLDGFEGNPYLPQGCQDIDECKNGSHEC 305
           CS  ++K NC ICG N+++I FL+DGSEYRCQCL+GFEGNPYLPQGCQDIDECKNGSH+C
Sbjct: 246 CSKGENKGNCSICGLNAERIRFLDDGSEYRCQCLEGFEGNPYLPQGCQDIDECKNGSHKC 305

Query: 306 KFIDKCVNTPGNYTCNCPKNYKGDGKRGGEGCTPNSKSFIQIIIGIGVGFIVLVIASTWL 365
           K+ D C NTPGNYTC+CPKN+KGDGK GGEGCTPN  S I IIIG  VG  VLVI   WL
Sbjct: 306 KYKDLCFNTPGNYTCHCPKNHKGDGKYGGEGCTPNFMSSIHIIIGTSVGLAVLVIGGIWL 365

Query: 366 YLIYKKWKFIQRKEKFFEKNGGFMLQHHLSQRQSPADMLRIFTQEELEKATNKYDESAVV 425
           YL Y++WKFIQ+KEKFF++NGG MLQ HLSQ QSP DML+IFT+EELEKATNKYDESAVV
Sbjct: 366 YLGYRRWKFIQQKEKFFKRNGGLMLQQHLSQWQSP-DMLKIFTREELEKATNKYDESAVV 425

Query: 426 GRGGFGTVYKGVLDDGLTVAIKKSKMVDQSQTAQFINEVIVLSQINHRNVVKLLGCCLET 485
           G+GG+GTVYKGVLDDG TVAIKKSK+V+QSQT QFINEVI+LSQINHRNVVKL+GCCLET
Sbjct: 426 GKGGYGTVYKGVLDDGSTVAIKKSKLVNQSQTNQFINEVIILSQINHRNVVKLIGCCLET 485

Query: 486 QVPLLVYEFITNGTLFDHIHDKIKHVSLSWEARLKIASETAGVLSYLHSSASTPIIHRDI 545
           +VPLLVYEFITNGTL +HIH K   V L W  RLKIASE A VLSYLH SASTPIIHRDI
Sbjct: 486 KVPLLVYEFITNGTLSEHIHSKTNQVRLPWATRLKIASEIASVLSYLHYSASTPIIHRDI 545

Query: 546 KSTNILLDDNCTAKVSDFGASKLVPLDHTQISTMVQGTLGYLDPEYLLTSELTEKSDVYS 605
           KS NILLD N TAKVSDFG SKLVPLD TQISTMVQGT+GYLDPEY LTSELTEKSDVYS
Sbjct: 546 KSNNILLDQNYTAKVSDFGTSKLVPLDRTQISTMVQGTIGYLDPEYFLTSELTEKSDVYS 605

Query: 606 FGIVLLELITGKKAVCFAGPEAERNLAMYVLCAMKEDRLEEVVEKEMATEGNFEQIKEVA 665
           FGIVLLELITGKKAVCF GPE ER+LAMYVLCAM+EDR+EEV+EK MATE NFE+IK+V 
Sbjct: 606 FGIVLLELITGKKAVCFDGPEEERSLAMYVLCAMEEDRVEEVMEKGMATEENFEEIKKVV 665

Query: 666 KVAKECVRVKGEERPSMKEVAMELEGLRLKQVEHL----WA--KNSLLGGALGTSQLVVS 725
           ++ ++C+RVK +ERPSMKEVAMELEG  L Q++H+    W+   N LL GA  T+Q+V +
Sbjct: 666 ELGRKCLRVKRDERPSMKEVAMELEG--LTQIQHIEFRSWSGRNNFLLDGASNTTQVVTT 725

Query: 726 GGMNAMDNSIKDQILSHIPHGR 736
                 D S+K + L+ +  GR
Sbjct: 726 D----TDESMKAEDLTFVNDGR 739

BLAST of Sgr014327 vs. ExPASy TrEMBL
Match: A0A1S3B3R7 (wall-associated receptor kinase 2-like OS=Cucumis melo OX=3656 GN=LOC103485671 PE=4 SV=1)

HSP 1 Score: 1040.4 bits (2689), Expect = 3.5e-300
Identity = 526/742 (70.89%), Postives = 610/742 (82.21%), Query Frame = 0

Query: 6   EKLVRLMTLLSTALVATAATGS--SDCERRCGNLEIPYPFGMKEGCYLNKNFSITCNKTH 65
           E L++L+ LLS  L A        + C+  CGNL IPYPFG+K+ CYL++ FSITCNKT 
Sbjct: 6   EDLMKLIILLSGTLAAAVEVAKVLTGCDEHCGNLRIPYPFGIKKRCYLDQAFSITCNKT- 65

Query: 66  YDPPKAFLGDGNIDLTNISIIHGELRILQYVARDCYTQNGSNIFN-NPTINISEFTISHT 125
           Y PPK FL D NI++TNISII G+L I Q+VARDCYT+NG +  N  P + +++F IS+T
Sbjct: 66  YHPPKPFLQDSNIEITNISIIQGQLHIKQFVARDCYTKNGPSESNTRPFLLLNKFRISNT 125

Query: 126 KNKFTVIGCDTYAFISGQLEGDSYRSGCMSLCGNSTT-TIRDGSCSGNGCCQLEIPKGLK 185
            NKF VIGCDTYA+I G++EG+SY+SGCM+LCGN+ T  I+DGSCSGNGCCQL+IPKGLK
Sbjct: 126 DNKFIVIGCDTYAYIYGEIEGESYKSGCMALCGNNNTKIIKDGSCSGNGCCQLQIPKGLK 185

Query: 186 DLNLKVNSFENHTHVLNFNPCGYAFVIQQDNFTFSSTYIRNFTQEEVPMVLDWAIPTDT- 245
            L L+V SF+NH+ VL FNPCGYAFVIQQD FTFS  YI NFTQEEVP+VLDW IPT+T 
Sbjct: 186 ILELEVRSFDNHSEVLKFNPCGYAFVIQQDKFTFSKKYIYNFTQEEVPLVLDWGIPTNTS 245

Query: 246 CSTADDKTNC-ICGPNSKKIDFLNDGSEYRCQCLDGFEGNPYLPQGCQDIDECKNGSHEC 305
           CS  ++K NC ICG N+++I FL+DGSEYRCQCL+GFEGNPYLPQGCQDIDECKNGSH+C
Sbjct: 246 CSKGENKGNCSICGLNAERIRFLDDGSEYRCQCLEGFEGNPYLPQGCQDIDECKNGSHKC 305

Query: 306 KFIDKCVNTPGNYTCNCPKNYKGDGKRGGEGCTPNSKSFIQIIIGIGVGFIVLVIASTWL 365
           K+ D C NTPGNYTC+CPKN+KGDGK GGEGCTPN  S I IIIG  VG  VLVI   WL
Sbjct: 306 KYKDLCFNTPGNYTCHCPKNHKGDGKYGGEGCTPNFMSSIHIIIGTSVGLAVLVIGGIWL 365

Query: 366 YLIYKKWKFIQRKEKFFEKNGGFMLQHHLSQRQSPADMLRIFTQEELEKATNKYDESAVV 425
           YL Y++WKFIQ+KEKFF++NGG MLQ HLSQ QSP DML+IFT+EELEKATNKYDESAVV
Sbjct: 366 YLGYRRWKFIQQKEKFFKRNGGLMLQQHLSQWQSP-DMLKIFTREELEKATNKYDESAVV 425

Query: 426 GRGGFGTVYKGVLDDGLTVAIKKSKMVDQSQTAQFINEVIVLSQINHRNVVKLLGCCLET 485
           G+GG+GTVYKGVLDDG TVAIKKSK+V+QSQT QFINEVI+LSQINHRNVVKL+GCCLET
Sbjct: 426 GKGGYGTVYKGVLDDGSTVAIKKSKLVNQSQTNQFINEVIILSQINHRNVVKLIGCCLET 485

Query: 486 QVPLLVYEFITNGTLFDHIHDKIKHVSLSWEARLKIASETAGVLSYLHSSASTPIIHRDI 545
           +VPLLVYEFITNGTL +HIH K   V L W  RLKIASE A VLSYLH SASTPIIHRDI
Sbjct: 486 KVPLLVYEFITNGTLSEHIHSKTNQVRLPWATRLKIASEIASVLSYLHYSASTPIIHRDI 545

Query: 546 KSTNILLDDNCTAKVSDFGASKLVPLDHTQISTMVQGTLGYLDPEYLLTSELTEKSDVYS 605
           KS NILLD N TAKVSDFG SKLVPLD TQISTMVQGT+GYLDPEY LTSELTEKSDVYS
Sbjct: 546 KSNNILLDQNYTAKVSDFGTSKLVPLDRTQISTMVQGTIGYLDPEYFLTSELTEKSDVYS 605

Query: 606 FGIVLLELITGKKAVCFAGPEAERNLAMYVLCAMKEDRLEEVVEKEMATEGNFEQIKEVA 665
           FGIVLLELITGKKAVCF GPE ER+LAMYVLCAM+EDR+EEV+EK MATE NFE+IK+V 
Sbjct: 606 FGIVLLELITGKKAVCFDGPEEERSLAMYVLCAMEEDRVEEVMEKGMATEENFEEIKKVV 665

Query: 666 KVAKECVRVKGEERPSMKEVAMELEGLRLKQVEHL----WA--KNSLLGGALGTSQLVVS 725
           ++ ++C+RVK +ERPSMKEVAMELEG  L Q++H+    W+   N LL GA  T+Q+V +
Sbjct: 666 ELGRKCLRVKRDERPSMKEVAMELEG--LTQIQHIEFRSWSGRNNFLLDGASNTTQVVTT 725

Query: 726 GGMNAMDNSIKDQILSHIPHGR 736
                 D S+K + L+ +  GR
Sbjct: 726 D----TDESMKAEDLTFVNDGR 739

BLAST of Sgr014327 vs. TAIR 10
Match: AT1G21270.1 (wall-associated kinase 2 )

HSP 1 Score: 623.2 bits (1606), Expect = 2.6e-178
Identity = 326/680 (47.94%), Postives = 452/680 (66.47%), Query Frame = 0

Query: 29  DCERRCGNLEIPYPFGMKEGCYL--NKNFSITCNKTHYDPPKAFLGDGNIDLTNISIIHG 88
           +C+ RCGN+ + YPFG   GCY   +++F++TCN    +  K F   GN+ + N+S + G
Sbjct: 28  ECQTRCGNVAVEYPFGTSPGCYYPGDESFNLTCN----EQEKLFF--GNMPVINMS-LSG 87

Query: 89  ELRILQYVARDCYTQNGSNI-FNNPTINISEFTISHTKNKFTVIGCDTYAFISGQLEG-D 148
           +LR+    +R CY   G    +      +  FT+S   N+FTV+GC++YAF+  +  G +
Sbjct: 88  QLRVRLVRSRVCYDSQGKQTDYIAQRTTLGNFTLSEL-NRFTVVGCNSYAFL--RTSGVE 147

Query: 149 SYRSGCMSLCGNSTTTIRDGSCSGNGCCQLEIPKGLKDLNLKVNSFENHTHVLNFNPCGY 208
            Y +GC+S+C ++TT  ++GSCSG GCCQ+ +P+G   + +K +SF NH  V  FNPC Y
Sbjct: 148 KYSTGCISICDSATT--KNGSCSGEGCCQIPVPRGYSFVRVKPHSFHNHPTVHLFNPCTY 207

Query: 209 AFVIQQDNFTFSS----TYIRNFTQEEVPMVLDWAIPTDTCSTADDKTNCICGPNSKKID 268
           AF+++   F F +      +RN T    P+VLDW+I   TC   + +   +CG NS   D
Sbjct: 208 AFLVEDGMFDFHALEDLNNLRNVT--TFPVVLDWSIGDKTCKQVEYRG--VCGGNSTCFD 267

Query: 269 FLNDGSEYRCQCLDGFEGNPYLPQGCQDIDECKNGSHECKFIDKCVNTPGNYTCNCPKNY 328
               G+ Y C+CL+GFEGNPYLP GCQDI+EC +  H C     C NT G++ CNCP  Y
Sbjct: 268 -STGGTGYNCKCLEGFEGNPYLPNGCQDINECISSRHNCSEHSTCENTKGSFNCNCPSGY 327

Query: 329 KGDGKRGGEGCT----PNSKSFIQIIIGIGVGFIVLVIASTWLYLIYKKWKFIQRKEKFF 388
           + D       CT    P    + QI +G  +GF V+++  + L    K  K  + ++KFF
Sbjct: 328 RKDSL---NSCTRKVRPEYFRWTQIFLGTTIGFSVIMLGISCLQQKIKHRKNTELRQKFF 387

Query: 389 EKNGGFMLQHHLSQRQSPADMLRIFTQEELEKATNKYDESAVVGRGGFGTVYKGVLDDGL 448
           E+NGG ML   +S        ++IFT++ +++ATN Y ES ++G+GG GTVYKG+L D  
Sbjct: 388 EQNGGGMLIQRVSGAGPSNVDVKIFTEKGMKEATNGYHESRILGQGGQGTVYKGILPDNS 447

Query: 449 TVAIKKSKMVDQSQTAQFINEVIVLSQINHRNVVKLLGCCLETQVPLLVYEFITNGTLFD 508
            VAIKK+++ ++SQ  QFINEV+VLSQINHRNVVK+LGCCLET+VPLLVYEFI +GTLFD
Sbjct: 448 IVAIKKARLGNRSQVEQFINEVLVLSQINHRNVVKVLGCCLETEVPLLVYEFINSGTLFD 507

Query: 509 HIHDKIKHVSLSWEARLKIASETAGVLSYLHSSASTPIIHRDIKSTNILLDDNCTAKVSD 568
           H+H  +   SL+WE RL+IA+E AG L+YLHSSAS PIIHRDIK+ NILLD N TAKV+D
Sbjct: 508 HLHGSLYDSSLTWEHRLRIATEVAGSLAYLHSSASIPIIHRDIKTANILLDKNLTAKVAD 567

Query: 569 FGASKLVPLDHTQISTMVQGTLGYLDPEYLLTSELTEKSDVYSFGIVLLELITGKKAVCF 628
           FGAS+L+P+D  Q++T+VQGTLGYLDPEY  T  L EKSDVYSFG+VL+EL++G+KA+CF
Sbjct: 568 FGASRLIPMDKEQLTTIVQGTLGYLDPEYYNTGLLNEKSDVYSFGVVLMELLSGQKALCF 627

Query: 629 AGPEAERNLAMYVLCAMKEDRLEEVVEKEMATEGNFEQIKEVAKVAKECVRVKGEERPSM 688
             P   +NL      A K +R  E+++ ++  E N  +I+E A++A EC R+ GEERP M
Sbjct: 628 ERPHCPKNLVSCFASATKNNRFHEIIDGQVMNEDNQREIQEAARIAAECTRLMGEERPRM 687

Query: 689 KEVAMELEGLRLKQVEHLWA 697
           KEVA ELE LR+K  ++ W+
Sbjct: 688 KEVAAELEALRVKTTKYKWS 687

BLAST of Sgr014327 vs. TAIR 10
Match: AT1G21230.1 (wall associated kinase 5 )

HSP 1 Score: 596.3 bits (1536), Expect = 3.4e-170
Identity = 319/682 (46.77%), Postives = 441/682 (64.66%), Query Frame = 0

Query: 29  DCERRCGNLEIPYPFGMKEGCYL--NKNFSITCNKTHYDPPKAFLGDGNIDLTNISIIHG 88
           DC+ RCG++ I YPFG+  GCY   + +F+ITC +   D P       NI++ N +   G
Sbjct: 28  DCQTRCGDVPIDYPFGISTGCYYPGDDSFNITCEE---DKPNVL---SNIEVLNFN-HSG 87

Query: 89  ELRILQYVARDCYTQNGSNIFNNPTINISEFTISHTKNKFTVIGCDTYAFISGQLEGDSY 148
           +LR L   +  CY Q  +N F +    +   + S   NKFT++GC+ +A +S      +Y
Sbjct: 88  QLRGLIPRSTVCYDQQTNNDFESLWFRLDNLSFS-PNNKFTLVGCNAWALLS-TFGIQNY 147

Query: 149 RSGCMSLCGNSTTTIRDGSCSGNGCCQLE--IPKGLKDLNLKVNSFENHTHVLNFNPCGY 208
            +GCMSLC   T    +  C+G GCC+ E  IP     +  + + FEN T V +FNPC Y
Sbjct: 148 STGCMSLC--DTPPPPNSKCNGVGCCRTEVSIPLDSHRIETQPSRFENMTSVEHFNPCSY 207

Query: 209 AFVIQQDNFTFSSTY----IRNFTQEEVPMVLDWAIPTDTCSTADDKTNCICGPNSKKID 268
           AF ++   F FSS      +RN T+   P++LDW+I   TC     +   ICG NS   D
Sbjct: 208 AFFVEDGMFNFSSLEDLKDLRNVTR--FPVLLDWSIGNQTCEQVVGRN--ICGGNSTCFD 267

Query: 269 FLNDGSEYRCQCLDGFEGNPYLPQGCQDIDECKNGSHECKFIDKCVNTPGNYTCNCPKNY 328
               G  Y C+CL GF+GNPYL  GCQDI+EC    H C     C NT G++ C CP   
Sbjct: 268 STR-GKGYNCKCLQGFDGNPYLSDGCQDINECTTRIHNCSDTSTCENTLGSFHCQCPSG- 327

Query: 329 KGDGKRGGEGC------TPNSKSFIQIIIGIGVGFIVLVIASTWLYLIYKKWKFIQRKEK 388
             D       C       P    +  +++G  +GF+++++  +++    +  K  + +++
Sbjct: 328 -SDLNTTTMSCIDTPKEEPKYLGWTTVLLGTTIGFLIILLTISYIQQKMRHRKNTELRQQ 387

Query: 389 FFEKNGGFMLQHHLSQRQSPADMLRIFTQEELEKATNKYDESAVVGRGGFGTVYKGVLDD 448
           FFE+NGG ML   LS        ++IFT+E +++AT+ Y+ES ++G+GG GTVYKG+L D
Sbjct: 388 FFEQNGGGMLIQRLSGAGPSNVDVKIFTEEGMKEATDGYNESRILGQGGQGTVYKGILQD 447

Query: 449 GLTVAIKKSKMVDQSQTAQFINEVIVLSQINHRNVVKLLGCCLETQVPLLVYEFITNGTL 508
              VAIKK+++ D+SQ  QFINEV+VLSQINHRNVVKLLGCCLET+VPLLVYEFI++GTL
Sbjct: 448 NSIVAIKKARLGDRSQVEQFINEVLVLSQINHRNVVKLLGCCLETEVPLLVYEFISSGTL 507

Query: 509 FDHIHDKIKHVSLSWEARLKIASETAGVLSYLHSSASTPIIHRDIKSTNILLDDNCTAKV 568
           FDH+H  +   SL+WE RL+IA E AG L+YLHS AS PIIHRD+K+ NILLD+N TAKV
Sbjct: 508 FDHLHGSMFDSSLTWEHRLRIAIEVAGTLAYLHSYASIPIIHRDVKTANILLDENLTAKV 567

Query: 569 SDFGASKLVPLDHTQISTMVQGTLGYLDPEYLLTSELTEKSDVYSFGIVLLELITGKKAV 628
           +DFGAS+L+P+D  Q++TMVQGTLGYLDPEY  T  L EKSDVYSFG+VL+EL++G+KA+
Sbjct: 568 ADFGASRLIPMDQEQLTTMVQGTLGYLDPEYYNTGLLNEKSDVYSFGVVLMELLSGEKAL 627

Query: 629 CFAGPEAERNLAMYVLCAMKEDRLEEVVEKEMATEGNFEQIKEVAKVAKECVRVKGEERP 688
           CF  P++ ++L  Y + AMKE+RL E+++ ++  E N  +I+E A++A EC R+ GEERP
Sbjct: 628 CFERPQSSKHLVSYFVSAMKENRLHEIIDGQVMNEYNQREIQESARIAVECTRIMGEERP 687

Query: 689 SMKEVAMELEGLRLKQVEHLWA 697
           SMKEVA ELE LR+K  +H W+
Sbjct: 688 SMKEVAAELEALRVKTTKHQWS 691

BLAST of Sgr014327 vs. TAIR 10
Match: AT1G21250.1 (cell wall-associated kinase )

HSP 1 Score: 584.3 bits (1505), Expect = 1.3e-166
Identity = 322/741 (43.45%), Postives = 457/741 (61.67%), Query Frame = 0

Query: 13  TLLSTALVATAATGSSDCERRCGNLEIPYPFGMKEGCYL--NKNFSITCNKTHYDPPKAF 72
           +L  T LV        +C+ +CGN+ I YPFG+  GCY   N++FSITC +   D P   
Sbjct: 15  SLACTQLVKGQHQPGENCQNKCGNITIEYPFGISSGCYYPGNESFSITCKE---DRPHVL 74

Query: 73  LGDGNIDLTNISIIHGELRILQYVARDCYTQNGSNIFNNPTINISEFTISHTKNKFTVIG 132
               +I++ N +   G+L++L   +  CY + G     + +  +   ++S   NK T +G
Sbjct: 75  ---SDIEVANFN-HSGQLQVLLNRSSTCYDEQGKKTEEDSSFTLENLSLS-ANNKLTAVG 134

Query: 133 CDTYAFISGQLEGDSYRSGCMSLCGNSTTTIRDGSCSGNGCCQLEIPKGLKDLNLKVNS- 192
           C+  + +       +Y + C+SLC +      DG C+G GCC++++   L     +  S 
Sbjct: 135 CNALSLLD-TFGMQNYSTACLSLCDSPPEA--DGECNGRGCCRVDVSAPLDSYTFETTSG 194

Query: 193 -FENHTHVLNFNPCGYAFVIQQDNFTFSST----YIRNFTQEEVPMVLDWAIPTDTCSTA 252
             ++ T   +F+PC YAF+++ D F FSST     +RN  +   P++LDW++   TC   
Sbjct: 195 RIKHMTSFHDFSPCTYAFLVEDDKFNFSSTEDLLNLRNVMR--FPVLLDWSVGNQTCEQV 254

Query: 253 DDKTNCICGPNSKKIDFLNDGSEYRCQCLDGFEGNPYLPQGCQDIDECKNGS----HECK 312
              +  ICG NS  +D     + Y C+C +GF+GNPYL  GCQD++EC   S    H C 
Sbjct: 255 G--STSICGGNSTCLD-STPRNGYICRCNEGFDGNPYLSAGCQDVNECTTSSTIHRHNCS 314

Query: 313 FIDKCVNTPGNYTCNCPKNYKGDGKRGGEGCTPNSKSFIQIIIGIGVGFIVLVIASTWLY 372
               C N  G + C C   Y+ D       C     ++  I++   +GF+V+++    + 
Sbjct: 315 DPKTCRNKVGGFYCKCQSGYRLDTTT--MSCKRKEFAWTTILLVTTIGFLVILLGVACIQ 374

Query: 373 LIYKKWKFIQRKEKFFEKNGGFMLQHHLSQRQSPADMLRIFTQEELEKATNKYDESAVVG 432
              K  K  + +E+FFE+NGG ML   LS        ++IFT++ ++KATN Y ES ++G
Sbjct: 375 QRMKHLKDTKLREQFFEQNGGGMLTQRLSGAGPSNVDVKIFTEDGMKKATNGYAESRILG 434

Query: 433 RGGFGTVYKGVLDDGLTVAIKKSKMVDQSQTAQFINEVIVLSQINHRNVVKLLGCCLETQ 492
           +GG GTVYKG+L D   VAIKK+++ D SQ  QFINEV+VLSQINHRNVVKLLGCCLET+
Sbjct: 435 QGGQGTVYKGILPDNSIVAIKKARLGDSSQVEQFINEVLVLSQINHRNVVKLLGCCLETE 494

Query: 493 VPLLVYEFITNGTLFDHIHDKIKHVSLSWEARLKIASETAGVLSYLHSSASTPIIHRDIK 552
           VPLLVYEFITNGTLFDH+H  +   SL+WE RLKIA E AG L+YLHSSAS PIIHRDIK
Sbjct: 495 VPLLVYEFITNGTLFDHLHGSMIDSSLTWEHRLKIAIEVAGTLAYLHSSASIPIIHRDIK 554

Query: 553 STNILLDDNCTAKVSDFGASKLVPLDHTQISTMVQGTLGYLDPEYLLTSELTEKSDVYSF 612
           + NILLD N TAKV+DFGAS+L+P+D  ++ TMVQGTLGYLDPEY  T  L EKSDVYSF
Sbjct: 555 TANILLDVNLTAKVADFGASRLIPMDKEELETMVQGTLGYLDPEYYNTGLLNEKSDVYSF 614

Query: 613 GIVLLELITGKKAVCFAGPEAERNLAMYVLCAMKEDRLEEVVEKEMATEGNFEQIKEVAK 672
           G+VL+EL++G+KA+CF  P++ ++L  Y   A KE+RL+E++  E+  E N ++I+E A+
Sbjct: 615 GVVLMELLSGQKALCFKRPQSSKHLVSYFATATKENRLDEIIGGEVMNEDNLKEIQEAAR 674

Query: 673 VAKECVRVKGEERPSMKEVAMELEGLRLKQVEHLWA------KNSLLGGALGTSQLVVSG 732
           +A EC R+ GEERP MKEVA +LE LR+++ +H W+         L+GG + ++Q   S 
Sbjct: 675 IAAECTRLMGEERPRMKEVAAKLEALRVEKTKHKWSDQYPEENEHLIGGHILSAQGETSS 734

Query: 733 GMNAMDNSIKDQILSHIPHGR 736
            +    +SIK+  +  I  GR
Sbjct: 735 SIGY--DSIKNVAILDIETGR 735

BLAST of Sgr014327 vs. TAIR 10
Match: AT1G21240.1 (wall associated kinase 3 )

HSP 1 Score: 581.6 bits (1498), Expect = 8.6e-166
Identity = 334/755 (44.24%), Postives = 463/755 (61.32%), Query Frame = 0

Query: 8   LVRLMTLLSTALVATAATGSSDCERRCGNLEIPYPFGMKEGCYL--NKNFSITCNKTHYD 67
           LV +  L  T LV        DC+ +CGN+ I YPFG+  GCY   + NF++TC      
Sbjct: 9   LVVIFFLAYTQLVKGQHQPREDCKLKCGNVTIEYPFGISTGCYYPGDDNFNLTC-----V 68

Query: 68  PPKAFLGDGNIDLTNISIIHGELRILQYVARDCYTQNGSNIFNNPTINI---SEFTISHT 127
             +  L  G I +TNIS   G + +L     +CY Q   N  N   +     S F++S +
Sbjct: 69  VEEKLLLFGIIQVTNIS-HSGHVSVLFERFSECYEQ--KNETNGTALGYQLGSSFSLS-S 128

Query: 128 KNKFTVIGCDTYAFISGQLEGDSYRSGCMSLCGNSTTTIRDGSCSGNGCCQLE------- 187
            NKFT++GC+  + +S      +Y +GC+SLC +      +G C+G GCC  E       
Sbjct: 129 NNKFTLVGCNALSLLS-TFGKQNYSTGCLSLCNSQPEA--NGRCNGVGCCTTEDFSVPFD 188

Query: 188 ---IPKGLKDLNLKVNSFEN--HTHVLNFNPCGYAFVIQQDNFTFSST----YIRNFTQE 247
                 G   L  +VN+  +  +T V  FNPC YAF+++   F F S+     +RN T+ 
Sbjct: 189 SDTFQFGSVRLRNQVNNSLDLFNTSVYQFNPCTYAFLVEDGKFNFDSSKDLKNLRNVTR- 248

Query: 248 EVPMVLDWAIPTDTCSTADDKTNCICGPNSKKIDFLNDGSEYRCQCLDGFEGNPYLPQGC 307
             P+ LDW+I   TC  A   +  ICG NS   +       Y C+C +G++GNPY  +GC
Sbjct: 249 -FPVALDWSIGNQTCEQAG--STRICGKNSSCYNSTTRNG-YICKCNEGYDGNPYRSEGC 308

Query: 308 QDIDECKNGSHECKFIDKCVNTPGNYTCNCPKNYKGDGKRGGEGCTPNSKSFIQIIIGIG 367
           +DIDEC + +H C     C N  G + C CP  Y          CT       +I + I 
Sbjct: 309 KDIDECISDTHNCSDPKTCRNRDGGFDCKCPSGY---DLNSSMSCTRPEYKRTRIFLVII 368

Query: 368 VGFIVLVIASTWLYLIYKKWKFIQRKEKFFEKNGGFMLQHHLSQRQSPADMLRIFTQEEL 427
           +G +VL++A+  +    K+ K+ + + +FFE+NGG ML   LS         +IFT+E +
Sbjct: 369 IGVLVLLLAAICIQHATKQRKYTKLRRQFFEQNGGGMLIQRLSGAGLSNIDFKIFTEEGM 428

Query: 428 EKATNKYDESAVVGRGGFGTVYKGVLDDGLTVAIKKSKMVDQSQTAQFINEVIVLSQINH 487
           ++ATN YDES ++G+GG GTVYKG+L D   VAIKK+++ D  Q  QFI+EV+VLSQINH
Sbjct: 429 KEATNGYDESRILGQGGQGTVYKGILPDNTIVAIKKARLADSRQVDQFIHEVLVLSQINH 488

Query: 488 RNVVKLLGCCLETQVPLLVYEFITNGTLFDHIHDKIKHVSLSWEARLKIASETAGVLSYL 547
           RNVVK+LGCCLET+VPLLVYEFITNGTLFDH+H  I   SL+WE RL+IA E AG L+YL
Sbjct: 489 RNVVKILGCCLETEVPLLVYEFITNGTLFDHLHGSIFDSSLTWEHRLRIAIEVAGTLAYL 548

Query: 548 HSSASTPIIHRDIKSTNILLDDNCTAKVSDFGASKLVPLDHTQISTMVQGTLGYLDPEYL 607
           HSSAS PIIHRDIK+ NILLD+N TAKV+DFGASKL+P+D  Q++TMVQGTLGYLDPEY 
Sbjct: 549 HSSASIPIIHRDIKTANILLDENLTAKVADFGASKLIPMDKEQLTTMVQGTLGYLDPEYY 608

Query: 608 LTSELTEKSDVYSFGIVLLELITGKKAVCFAGPEAERNLAMYVLCAMKEDRLEEVVEKEM 667
            T  L EKSDVYSFG+VL+EL++G+KA+CF  P+A ++L  Y + A +E+RL E+++ ++
Sbjct: 609 TTGLLNEKSDVYSFGVVLMELLSGQKALCFERPQASKHLVSYFVSATEENRLHEIIDDQV 668

Query: 668 ATEGNFEQIKEVAKVAKECVRVKGEERPSMKEVAMELEGLRLKQVEHLWA------KNSL 727
             E N ++I+E A++A EC R+ GEERP MKEVA +LE LR+++ +H W+         L
Sbjct: 669 LNEDNLKEIQEAARIAAECTRLMGEERPRMKEVAAKLEALRVEKTKHKWSDQYPEENEHL 728

Query: 728 LGGALGTSQLVVSGGMNAMDNSIKDQILSHIPHGR 736
           +GG + ++Q   S  +    +SIK+  +  I  GR
Sbjct: 729 IGGHILSAQGETSSSIGY--DSIKNVAILDIETGR 741

BLAST of Sgr014327 vs. TAIR 10
Match: AT1G21210.1 (wall associated kinase 4 )

HSP 1 Score: 547.7 bits (1410), Expect = 1.4e-155
Identity = 299/689 (43.40%), Postives = 433/689 (62.84%), Query Frame = 0

Query: 30  CERRCGNLEIPYPFGMKEGCYL--NKNFSITCNKTHYDPPKAFLGDGNIDLTNISIIHGE 89
           C  +CGN+ + YPFG   GC+   + +F+++C   +      + G   +++++ S    +
Sbjct: 28  CPEKCGNVTLEYPFGFSPGCWRAEDPSFNLSCVNENL----FYKGLEVVEISHSS----Q 87

Query: 90  LRILQYVARDCYTQNGSNIFNNPTI---NISEFTISHTKNKFTVIGCDTYAFISGQLEGD 149
           LR+L   +  CY   G   F   T    N+   T+S   N  T +GC++YAF+S    G 
Sbjct: 88  LRVLYPASYICYNSKGK--FAKGTYYWSNLGNLTLS-GNNTITALGCNSYAFVSS--NGT 147

Query: 150 SYRS-GCMSLCGNSTTTIRDGSCSGNGCCQLEIPKGLKDLNLKVNSFENHTHV--LNFNP 209
              S GC+S C ++ +   +G C+G GCCQ  +P G   L ++   F+N T V  ++   
Sbjct: 148 RRNSVGCISAC-DALSHEANGECNGEGCCQNPVPAGNNWLIVRSYRFDNDTSVQPISEGQ 207

Query: 210 CGYAFVIQQDNFTFSSTYIRNFTQEE---VPMVLDWAIPTDTCSTADDK---TNCICGPN 269
           C YAF+++   F ++++   ++ Q      P+VLDW+I  +TC    +K    N IC  +
Sbjct: 208 CIYAFLVENGKFKYNASDKYSYLQNRNVGFPVVLDWSIRGETCGQVGEKKCGVNGICSNS 267

Query: 270 SKKIDFLNDGSEYRCQCLDGFEGNPYLPQGCQDIDECKNGS----HECKFIDKCVNTPGN 329
           +        G  Y C+C  GF+GNPYL  GCQDI+EC   +    H C     C N  G+
Sbjct: 268 A-------SGIGYTCKCKGGFQGNPYLQNGCQDINECTTANPIHKHNCSGDSTCENKLGH 327

Query: 330 YTCNCPKNYKGDGKRGGEGCTPNSK----SFIQIIIGIGVGFIVLVIASTWLYLIYKKWK 389
           + CNC   Y+ +       C P        +  I++G  +GF+V+++A + +    K  K
Sbjct: 328 FRCNCRSRYELNTTT--NTCKPKGNPEYVEWTTIVLGTTIGFLVILLAISCIEHKMKNTK 387

Query: 390 FIQRKEKFFEKNGGFMLQHHLSQRQSPADMLRIFTQEELEKATNKYDESAVVGRGGFGTV 449
             + +++FFE+NGG ML   LS        ++IFT+E +++AT+ YDE+ ++G+GG GTV
Sbjct: 388 DTELRQQFFEQNGGGMLMQRLSGAGPSNVDVKIFTEEGMKEATDGYDENRILGQGGQGTV 447

Query: 450 YKGVLDDGLTVAIKKSKMVDQSQTAQFINEVIVLSQINHRNVVKLLGCCLETQVPLLVYE 509
           YKG+L D   VAIKK+++ D SQ  QFINEV+VLSQINHRNVVKLLGCCLET+VPLLVYE
Sbjct: 448 YKGILPDNSIVAIKKARLGDNSQVEQFINEVLVLSQINHRNVVKLLGCCLETEVPLLVYE 507

Query: 510 FITNGTLFDHIHDKIKHVSLSWEARLKIASETAGVLSYLHSSASTPIIHRDIKSTNILLD 569
           FI++GTLFDH+H  +   SL+WE RL++A E AG L+YLHSSAS PIIHRDIK+ NILLD
Sbjct: 508 FISSGTLFDHLHGSMFDSSLTWEHRLRMAVEIAGTLAYLHSSASIPIIHRDIKTANILLD 567

Query: 570 DNCTAKVSDFGASKLVPLDHTQISTMVQGTLGYLDPEYLLTSELTEKSDVYSFGIVLLEL 629
           +N TAKV+DFGAS+L+P+D   ++TMVQGTLGYLDPEY  T  L EKSDVYSFG+VL+EL
Sbjct: 568 ENLTAKVADFGASRLIPMDKEDLATMVQGTLGYLDPEYYNTGLLNEKSDVYSFGVVLMEL 627

Query: 630 ITGKKAVCFAGPEAERNLAMYVLCAMKEDRLEEVVEKEMATEGNFEQIKEVAKVAKECVR 689
           ++G+KA+CF  P+  +++  Y   A KE+RL E+++ ++  E N  +I++ A++A EC R
Sbjct: 628 LSGQKALCFERPQTSKHIVSYFASATKENRLHEIIDGQVMNENNQREIQKAARIAVECTR 687

Query: 690 VKGEERPSMKEVAMELEGLRLKQVEHLWA 697
           + GEERP MKEVA ELE LR+ + +H W+
Sbjct: 688 LTGEERPGMKEVAAELEALRVTKTKHKWS 693

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038885912.11.6e-30773.45wall-associated receptor kinase 2-like [Benincasa hispida][more]
XP_038886240.12.8e-30771.41wall-associated receptor kinase 2-like [Benincasa hispida][more]
XP_022989770.11.2e-30570.89wall-associated receptor kinase 2-like [Cucurbita maxima][more]
XP_023512416.12.0e-30571.56wall-associated receptor kinase 2-like [Cucurbita pepo subsp. pepo][more]
XP_022960687.15.4e-30371.12wall-associated receptor kinase 3-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9LMP13.6e-17747.94Wall-associated receptor kinase 2 OS=Arabidopsis thaliana OX=3702 GN=WAK2 PE=1 S... [more]
Q9LMN74.8e-16946.77Wall-associated receptor kinase 5 OS=Arabidopsis thaliana OX=3702 GN=WAK5 PE=2 S... [more]
Q391911.9e-16543.45Wall-associated receptor kinase 1 OS=Arabidopsis thaliana OX=3702 GN=WAK1 PE=1 S... [more]
Q9LMN81.2e-16444.24Wall-associated receptor kinase 3 OS=Arabidopsis thaliana OX=3702 GN=WAK3 PE=2 S... [more]
Q9LMN61.9e-15443.40Wall-associated receptor kinase 4 OS=Arabidopsis thaliana OX=3702 GN=WAK4 PE=2 S... [more]
Match NameE-valueIdentityDescription
A0A6J1JNA55.6e-30670.89wall-associated receptor kinase 2-like OS=Cucurbita maxima OX=3661 GN=LOC1114868... [more]
A0A6J1H8432.6e-30371.12wall-associated receptor kinase 3-like OS=Cucurbita moschata OX=3662 GN=LOC11146... [more]
A0A6J1CJM01.6e-30070.35putative wall-associated receptor kinase-like 16 OS=Momordica charantia OX=3673 ... [more]
A0A5D3DKI33.5e-30070.89Wall-associated receptor kinase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A1S3B3R73.5e-30070.89wall-associated receptor kinase 2-like OS=Cucumis melo OX=3656 GN=LOC103485671 P... [more]
Match NameE-valueIdentityDescription
AT1G21270.12.6e-17847.94wall-associated kinase 2 [more]
AT1G21230.13.4e-17046.77wall associated kinase 5 [more]
AT1G21250.11.3e-16643.45cell wall-associated kinase [more]
AT1G21240.18.6e-16644.24wall associated kinase 3 [more]
AT1G21210.11.4e-15543.40wall associated kinase 4 [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001881EGF-like calcium-binding domainSMARTSM00179egfca_6coord: 288..332
e-value: 6.9E-4
score: 27.3
IPR001881EGF-like calcium-binding domainPFAMPF07645EGF_CAcoord: 288..324
e-value: 1.3E-7
score: 31.7
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 413..691
e-value: 2.3E-31
score: 120.2
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 413..696
score: 38.887592
IPR001245Serine-threonine/tyrosine-protein kinase, catalytic domainPFAMPF07714PK_Tyr_Ser-Thrcoord: 418..683
e-value: 6.3E-48
score: 163.3
IPR025287Wall-associated receptor kinase, galacturonan-binding domainPFAMPF13947GUB_WAK_bindcoord: 30..90
e-value: 7.2E-9
score: 36.0
NoneNo IPR availableGENE3D2.10.25.10Laminincoord: 237..331
e-value: 7.9E-13
score: 50.6
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 489..699
e-value: 1.9E-58
score: 199.2
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 384..488
e-value: 2.4E-31
score: 109.9
NoneNo IPR availablePANTHERPTHR27005WALL-ASSOCIATED RECEPTOR KINASE-LIKE 21coord: 15..700
NoneNo IPR availablePANTHERPTHR27005:SF341WALL-ASSOCIATED RECEPTOR KINASE 2-LIKE ISOFORM X2coord: 15..700
NoneNo IPR availableCDDcd14066STKc_IRAKcoord: 419..686
e-value: 1.39447E-98
score: 303.425
NoneNo IPR availableSUPERFAMILY57196EGF/Laminincoord: 287..323
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 534..546
IPR018097EGF-like calcium-binding, conserved sitePROSITEPS01187EGF_CAcoord: 288..314
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 419..442
IPR000152EGF-type aspartate/asparagine hydroxylation sitePROSITEPS00010ASX_HYDROXYLcoord: 305..316
IPR000742EGF-like domainPROSITEPS50026EGF_3coord: 288..323
score: 11.000376
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 392..683

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr014327.1Sgr014327.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0007166 cell surface receptor signaling pathway
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0030247 polysaccharide binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity