Sgr014308 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr014308 vs. NCBI nr
Match: XP_022141684.1 (NAD kinase 2, chloroplastic isoform X1 [Momordica charantia])

HSP 1 Score: 1859.7 bits (4816), Expect = 0.0e+00
Identity = 924/1016 (90.94%), Postives = 957/1016 (94.19%), Query Frame = 0

Query: 1    MVVCCFEWHLDAIAVGMNRSLPATVIHSYLSPLRPLLPSCNARFLGFQLQTWKRLRRRLK 60
            MVVCCFEW LDAIAVGMNRSLPATVIHSYLSP RPLLPSCNARFLGFQLQTWKRLRRRLK
Sbjct: 1    MVVCCFEWQLDAIAVGMNRSLPATVIHSYLSPFRPLLPSCNARFLGFQLQTWKRLRRRLK 60

Query: 61   FAVTAELSKSLSLNSGSEFQLPWIGPVPGDIAEVEAYCRIFRTAERLHSVLMDTLCNPLT 120
            FAVTAELSKS+SLNSGSEFQLPWIGPVPGDIAEVEAYCRIFRTAERLHSVLMDTLCNPLT
Sbjct: 61   FAVTAELSKSVSLNSGSEFQLPWIGPVPGDIAEVEAYCRIFRTAERLHSVLMDTLCNPLT 120

Query: 121  GECSVSYDVKPGKNPLIEDKIVSVLGCIVSLLNKGREDVLSGRSSAMNTFQVANLDAMED 180
            GECSVSYD+ PG+NP+IEDKIVSVLGC+VSLLNKGREDVLSGRS+AMN FQ ANLD  ED
Sbjct: 121  GECSVSYDLTPGENPVIEDKIVSVLGCLVSLLNKGREDVLSGRSAAMNCFQAANLDVTED 180

Query: 181  DLPPLAAFRSEMKRCCESLHVALENFLTPGDDRSLDVWRKLQRLKNVCYDSGFSRGEAYP 240
             LPPLAAFRSEMKRCCESLHVALENFLTPGDDRSLDVW KLQRLKNVCYDSGF+RGE YP
Sbjct: 181  SLPPLAAFRSEMKRCCESLHVALENFLTPGDDRSLDVWGKLQRLKNVCYDSGFTRGEDYP 240

Query: 241  CHTLFANWNPVYLHNFKEETLAKKSDVAFWSGGQVTEEGLKWLIERGFKTIVDLRAETVK 300
            CHTLFANWNPVYLHNFKEETL K SDVAFWSGGQVTEEGLKWLIERGFKTIVDLRAETVK
Sbjct: 241  CHTLFANWNPVYLHNFKEETLEKNSDVAFWSGGQVTEEGLKWLIERGFKTIVDLRAETVK 300

Query: 301  DDFHTALLQDAIGSAKVKVIKIPVEVRTAPSVDQVEKFASLVSDGSNRLIYLHSKEGVWR 360
            DDF+ A L DAI SAKVKVIKIPVEVRTAP+VDQVEKF+SLVSDGSNR IYLHSKEGVWR
Sbjct: 301  DDFYNAFLHDAICSAKVKVIKIPVEVRTAPTVDQVEKFSSLVSDGSNRPIYLHSKEGVWR 360

Query: 361  TSAMISRWRQYVTRSGSQFVSNQTIGGDIPLRDTSPKLAHNQNGAKESLEIFTIQETFPR 420
            TSAMISRWRQYVTRS SQ VSNQTI GDIPLRDTS KL HNQNGAKESLEI  I+ETF  
Sbjct: 361  TSAMISRWRQYVTRSESQLVSNQTIAGDIPLRDTSSKLEHNQNGAKESLEISKIEETFTC 420

Query: 421  EEDNQSLLVESAHHSSIDVIGKTNYAETDKGSQNVNGAYNRPIPSQDLSSLRAVDSGEEY 480
            E+DNQSLLVESA H SID+I K N A+T+  SQ VNGA++ PIP+QDL+S RAVDSGEEY
Sbjct: 421  EKDNQSLLVESARHGSIDLISKKNCAQTETESQTVNGAFHGPIPTQDLTSPRAVDSGEEY 480

Query: 481  LSQFSIETDPLKAQIPPCNIFSRKEMSNFFRTKKISPPNYFNHQMKRKEKWPLSIELPAS 540
             SQ  IETDPLKAQIPPCN+FS+KEMSNFFRTKKISP NYFNH +K++EKW LS ELPA 
Sbjct: 481  PSQSGIETDPLKAQIPPCNVFSKKEMSNFFRTKKISPQNYFNHHLKKREKWLLSTELPAR 540

Query: 541  RIQRFSVDDSNLKSGFVEAENFTGSASVKDSSSKTHYLSTTKMKYVSGDSHVSANPVLNG 600
            RIQRFSVDDSNLKSGFVEA NF GS SVKD+SS THYLSTTKMKYV+GDSHVSANPVLNG
Sbjct: 541  RIQRFSVDDSNLKSGFVEAGNFNGSPSVKDTSSNTHYLSTTKMKYVNGDSHVSANPVLNG 600

Query: 601  LGVDGRNPLTTEASTAVGGTFQSKSEKKDLKSNGQATSVSGNVDMESVEGNMCASATGVV 660
            L VDGRN +T EASTAVG  FQS+SEKK+LKSNGQA SVSGNVDMESVEGNMCASATGVV
Sbjct: 601  LEVDGRNSVTIEASTAVGSKFQSESEKKELKSNGQAISVSGNVDMESVEGNMCASATGVV 660

Query: 661  RVQSRKKAEMFLVRTDGFSCAREKVTESSLAFTHPSTQQQMLMWKSTPKTVLLLKKLGQE 720
            RVQSR+KAEMFLVRTDGFSCAREKVTESSLAFTHPSTQQQMLMWKSTPKTVLLLKKLGQE
Sbjct: 661  RVQSRRKAEMFLVRTDGFSCAREKVTESSLAFTHPSTQQQMLMWKSTPKTVLLLKKLGQE 720

Query: 721  LMEEAKEAASFLYHQEKMNVLVEPDVHDIFARIPGFGFVQTFYIQDTSDLHEKVDFVACL 780
            LMEEAKE ASFL+HQEKM+VLVEPDVHDIFARIPGFGFVQTFY QDTSDLHEKVD VACL
Sbjct: 721  LMEEAKEVASFLHHQEKMHVLVEPDVHDIFARIPGFGFVQTFYSQDTSDLHEKVDLVACL 780

Query: 781  GGDGVILHASNLFRGAVPPVVSFNLGSLGFLTSHTFESYRQDLRQVIHGNDSRDGVYITL 840
            GGDGVILHASNLFRGAVPPVVSFNLGSLGFLTSHTF+SYRQDLRQVIHGNDSRDGVYITL
Sbjct: 781  GGDGVILHASNLFRGAVPPVVSFNLGSLGFLTSHTFDSYRQDLRQVIHGNDSRDGVYITL 840

Query: 841  RMRLQCEIFRNGKAIPGKLFNILNEVVVDRGSNPYLSKIECYEHDRLITKVQGDGVIVAT 900
            RMRLQCEIFRNGKAIPGKLFNILNEVVVDRGSNPYLSKIECYEHDRLITKVQGDGVIVAT
Sbjct: 841  RMRLQCEIFRNGKAIPGKLFNILNEVVVDRGSNPYLSKIECYEHDRLITKVQGDGVIVAT 900

Query: 901  PTGSTAYSTAAGGSMVHPNVPCMLFTPICPHSLSFRPVILPDSARLELKIPEDTRSNAWV 960
            PTGSTAYSTAAGGSMVHPNVPCMLFTPICPHSLSFRPVILPDSARLELKIPED RSNAWV
Sbjct: 901  PTGSTAYSTAAGGSMVHPNVPCMLFTPICPHSLSFRPVILPDSARLELKIPEDARSNAWV 960

Query: 961  SFDGKRRQQLSRGDSVRISMSSHPLPTVNKSDQTGDWFRSLIRCLNWNERLDQKAL 1017
            SFDGKRRQQLSRGDSVRISMS HPLPTVNKSDQTGDWFRSLIRCLNWNERLDQKAL
Sbjct: 961  SFDGKRRQQLSRGDSVRISMSRHPLPTVNKSDQTGDWFRSLIRCLNWNERLDQKAL 1016

BLAST of Sgr014308 vs. NCBI nr
Match: XP_038884758.1 (NAD kinase 2, chloroplastic [Benincasa hispida])

HSP 1 Score: 1742.6 bits (4512), Expect = 0.0e+00
Identity = 877/1002 (87.52%), Postives = 922/1002 (92.02%), Query Frame = 0

Query: 17   MNRSLPATVIHSYLSPLRPLLPSC-NARFLGFQLQTWKRLRRRLKFAVTAELSKSLSLNS 76
            MNRSLPA+VIHSYLSP RPL PSC NARFLGFQ QTWKR+RRRL+FAVTAELSKS SL+S
Sbjct: 1    MNRSLPASVIHSYLSPFRPLFPSCNNARFLGFQFQTWKRIRRRLRFAVTAELSKSASLHS 60

Query: 77   GSEFQLPWIGPVPGDIAEVEAYCRIFRTAERLHSVLMDTLCNPLTGECSVSYDVKPGKNP 136
            GS+FQLPW+GPVPGDIAEVEAYCRIFRTAERLHSVLMDTLCNP TGECSVSYDV PG+NP
Sbjct: 61   GSDFQLPWMGPVPGDIAEVEAYCRIFRTAERLHSVLMDTLCNPFTGECSVSYDVSPGENP 120

Query: 137  LIEDKIVSVLGCIVSLLNKGREDVLSGRSSAMNTFQVANLDAMEDDLPPLAAFRSEMKRC 196
            +IEDKIVSVLGC+VSLLNKGREDVLSGRSSAMN+F+ AN+D MED+LPPLAAFRSEMKRC
Sbjct: 121  VIEDKIVSVLGCLVSLLNKGREDVLSGRSSAMNSFRGANVDGMEDNLPPLAAFRSEMKRC 180

Query: 197  CESLHVALENFLTPGDDRSLDVWRKLQRLKNVCYDSGFSRGEAYPCHTLFANWNPVYLHN 256
            CESLHVALENFL PGD+RSLDVWRKLQRLKNVCYDSGF+RGE YPCHTLFANWNPVYL N
Sbjct: 181  CESLHVALENFLAPGDERSLDVWRKLQRLKNVCYDSGFTRGEDYPCHTLFANWNPVYLQN 240

Query: 257  FKEETLAKKSDVAFWSGGQVTEEGLKWLIERGFKTIVDLRAETVKDDFHTALLQDAIGSA 316
            FK+ET AK SDVAFWSGGQVTEEGLKWLIERGFKTIVDLRAETVKD+F++A L DAIGSA
Sbjct: 241  FKDETSAKNSDVAFWSGGQVTEEGLKWLIERGFKTIVDLRAETVKDEFYSASLHDAIGSA 300

Query: 317  KVKVIKIPVEVRTAPSVDQVEKFASLVSDGSNRLIYLHSKEGVWRTSAMISRWRQYVTRS 376
            KVKVIKIPVEVRTAP+VDQVEKFASLVSDGSN LIYLHSKEGVWRTSAMISRWRQY TRS
Sbjct: 301  KVKVIKIPVEVRTAPTVDQVEKFASLVSDGSNGLIYLHSKEGVWRTSAMISRWRQYATRS 360

Query: 377  GSQFVSNQTI-GGDIPLRDTSPKLAHNQNGAKESLEIFTIQETFPREEDNQSLLVESAHH 436
            GSQ VSNQTI  GDIPLRDTS KLAHN NGAKESLEI  I ETFP EED Q LL+ESAH 
Sbjct: 361  GSQIVSNQTIVPGDIPLRDTSSKLAHNHNGAKESLEISIIGETFPSEEDGQFLLLESAHQ 420

Query: 437  SSIDVIGKTNYAETDKGSQNVNGAYNRPIPSQDLSSLRAVDSGEEYLSQFSIETDPLKAQ 496
            SSI+   + NYAETD+ SQNVNGAYN P P+QD++SLRAVD+G        I+ DPLKAQ
Sbjct: 421  SSIN---RKNYAETDEVSQNVNGAYNGPSPTQDMTSLRAVDNG-------GIKIDPLKAQ 480

Query: 497  IPPCNIFSRKEMSNFFRTKKISPPNYFNHQMKRKEKWPLSIELPASRIQRFSVDDSNLKS 556
            IPPCNIFSRKEMSNFFRTK ISP  Y + +MK KEK  +S E+ AS +QR SV+DS+LKS
Sbjct: 481  IPPCNIFSRKEMSNFFRTKNISPQYYLHRRMKTKEK--ISTEISASGVQRSSVNDSDLKS 540

Query: 557  GFVEAENFTGSASVKDSSSKTHYLSTTKMKYVSGDSHVSANPVLNGLGVDGRNPLTTEAS 616
            G VEA NF G+ SVKDSSSKT YLSTTKM YV+GDSHVSANPVL GLGVDGRNPLTT AS
Sbjct: 541  GIVEAGNFNGNPSVKDSSSKTQYLSTTKMNYVNGDSHVSANPVLKGLGVDGRNPLTTVAS 600

Query: 617  TAVGGTFQSKSEKKDLKSNGQATSVSGNVDMESVEGNMCASATGVVRVQSRKKAEMFLVR 676
            T VGG   SKSE   L+SNGQATSVS N+++ESVEGNMCASATGVVRVQSR+KAEMFLVR
Sbjct: 601  TVVGGIVPSKSETNGLRSNGQATSVSSNINVESVEGNMCASATGVVRVQSRRKAEMFLVR 660

Query: 677  TDGFSCAREKVTESSLAFTHPSTQQQMLMWKSTPKTVLLLKKLGQELMEEAKEAASFLYH 736
            TDGFSCAREKVTESSLAFTHPSTQQQMLMWKSTPKTVLLLKKLGQELMEEAKE A FLYH
Sbjct: 661  TDGFSCAREKVTESSLAFTHPSTQQQMLMWKSTPKTVLLLKKLGQELMEEAKEVALFLYH 720

Query: 737  QEKMNVLVEPDVHDIFARIPGFGFVQTFYIQDTSDLHEKVDFVACLGGDGVILHASNLFR 796
            QEKMNVLVEPD+HDIFARIPGFGFVQTFY QDTSDLHEKVDFVACLGGDGVILHASNLFR
Sbjct: 721  QEKMNVLVEPDIHDIFARIPGFGFVQTFYSQDTSDLHEKVDFVACLGGDGVILHASNLFR 780

Query: 797  GAVPPVVSFNLGSLGFLTSHTFESYRQDLRQVIHGNDSRDGVYITLRMRLQCEIFRNGKA 856
            GAVPPVVSFNLGSLGFLTSHTF+SYRQDLRQVIHGNDS DGVYITLRMRLQCEIFRNGKA
Sbjct: 781  GAVPPVVSFNLGSLGFLTSHTFDSYRQDLRQVIHGNDSLDGVYITLRMRLQCEIFRNGKA 840

Query: 857  IPGKLFNILNEVVVDRGSNPYLSKIECYEHDRLITKVQGDGVIVATPTGSTAYSTAAGGS 916
            IPGKLFNILNEVVVDRGSNPYLSKIECYEHDRLITKVQGDGVIVATPTGSTAYSTAAGGS
Sbjct: 841  IPGKLFNILNEVVVDRGSNPYLSKIECYEHDRLITKVQGDGVIVATPTGSTAYSTAAGGS 900

Query: 917  MVHPNVPCMLFTPICPHSLSFRPVILPDSARLELKIPEDTRSNAWVSFDGKRRQQLSRGD 976
            MVHPNVPCMLFTPICPHSLSFRPVILPDSARLELKIPED RSNAWVSFDGKRRQQLSRGD
Sbjct: 901  MVHPNVPCMLFTPICPHSLSFRPVILPDSARLELKIPEDARSNAWVSFDGKRRQQLSRGD 960

Query: 977  SVRISMSSHPLPTVNKSDQTGDWFRSLIRCLNWNERLDQKAL 1017
            SVRISMS HPLPTVNKSDQTGDWFRSLIRCLNWNERLDQKAL
Sbjct: 961  SVRISMSRHPLPTVNKSDQTGDWFRSLIRCLNWNERLDQKAL 990

BLAST of Sgr014308 vs. NCBI nr
Match: XP_023552928.1 (LOW QUALITY PROTEIN: NAD kinase 2, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1741.9 bits (4510), Expect = 0.0e+00
Identity = 873/1020 (85.59%), Postives = 930/1020 (91.18%), Query Frame = 0

Query: 1    MVVCCFEWHLDAIAVGMNRSLPATVIHS--YLSPLRPLLPSC-NARFLGFQLQTWKRLRR 60
            M++CCF WHLDAIA+GMNRSLPATVIHS  YLSP RPLLPS  NARFLGFQL TW+R+RR
Sbjct: 1    MLLCCFVWHLDAIALGMNRSLPATVIHSYPYLSPFRPLLPSSNNARFLGFQLHTWRRIRR 60

Query: 61   RLKFAVTAELSKSLSLNSGSEFQLPWIGPVPGDIAEVEAYCRIFRTAERLHSVLMDTLCN 120
            RLKFAVTAELSKS+S NS SEFQL W+GPVPGDIAEVEAYCRIFRTAERLHSVLMDTLCN
Sbjct: 61   RLKFAVTAELSKSVSFNSASEFQLSWMGPVPGDIAEVEAYCRIFRTAERLHSVLMDTLCN 120

Query: 121  PLTGECSVSYDVKPGKNPLIEDKIVSVLGCIVSLLNKGREDVLSGRSSAMNTFQVANLDA 180
            P TGECSVSYDV PG+NP+IEDKIVSVLGCIVSLLNKG+EDVLSGRSSAMN F+  NLDA
Sbjct: 121  PFTGECSVSYDVTPGENPVIEDKIVSVLGCIVSLLNKGKEDVLSGRSSAMNAFRGDNLDA 180

Query: 181  MEDDLPPLAAFRSEMKRCCESLHVALENFLTPGDDRSLDVWRKLQRLKNVCYDSGFSRGE 240
            MED+LPPLAAFRSEMKRCCESLHVALENFLTP DDRS DVWRKLQRLKNVCYDSGF RGE
Sbjct: 181  MEDNLPPLAAFRSEMKRCCESLHVALENFLTPDDDRSWDVWRKLQRLKNVCYDSGFPRGE 240

Query: 241  AYPCHTLFANWNPVYLHNFKEETLAKKSDVAFWSGGQVTEEGLKWLIERGFKTIVDLRAE 300
             YPCHTLFANWNPVYLHN KE+  AK SDV+FWSGGQ+TEEGLKWLIERGFKTIVDLRAE
Sbjct: 241  DYPCHTLFANWNPVYLHNSKEDVSAKNSDVSFWSGGQITEEGLKWLIERGFKTIVDLRAE 300

Query: 301  TVKDDFHTALLQDAIGSAKVKVIKIPVEVRTAPSVDQVEKFASLVSDGSNRLIYLHSKEG 360
            TVKD F++A L DAIGS KVKVI+IPVEVRTAP+ DQVEKFASLVSDGSN LIYLHSKEG
Sbjct: 301  TVKDIFYSASLHDAIGSDKVKVIRIPVEVRTAPTADQVEKFASLVSDGSNGLIYLHSKEG 360

Query: 361  VWRTSAMISRWRQYVTRSGSQFVSNQTI-GGDIPLRDTSPKLAHNQNGAKESLEIFTIQE 420
            VWRTSAMISRWRQY TRSGSQFVSNQTI   DIPL DTSPKL  N NGAKESLEI T+++
Sbjct: 361  VWRTSAMISRWRQYATRSGSQFVSNQTIVPADIPLGDTSPKLVQNPNGAKESLEISTVEK 420

Query: 421  TFPREEDNQSLLVESAHHSSIDVIGKTNYAETDKGSQNVNGAYNRPIPSQDLSSLRAVDS 480
            T   +E+++SLL+ESA H SI+   + N AE DK  QN NGA+N  IP+QDL+SLRA  +
Sbjct: 421  TSSCDENSRSLLLESADHRSIN---RKNNAEADKIIQNENGAFNGSIPTQDLTSLRADHN 480

Query: 481  GEEYLSQFSIETDPLKAQIPPCNIFSRKEMSNFFRTKKISPPNYFNHQMKRKEKWPLSIE 540
            GEE+LSQFS+ TDPLKAQIPPCNIFSRKEMSNFFRTKKISP NY + +MK  EK P S E
Sbjct: 481  GEEHLSQFSVATDPLKAQIPPCNIFSRKEMSNFFRTKKISPQNYLHRKMKTNEKRPPSTE 540

Query: 541  LPASRIQRFSVDDSNLKSGFVEAENFTGSASVKDSSSKTHYLSTTKMKYVSGDSHVSANP 600
            LP SR+Q+FSVD+S+LKSG VEA N  G  +VK++ S THYLSTT M+YV+GDSHVS+NP
Sbjct: 541  LPMSRVQKFSVDNSDLKSGLVEAGNTNGRPTVKETFSTTHYLSTTNMRYVNGDSHVSSNP 600

Query: 601  VLNGLGVDGRNPLTTEASTAVGGTFQSKSEKKDLKSNGQATSVSGNVDMESVEGNMCASA 660
            V N LGVDGRNPL TEASTAVG  FQSK+E   LKSNGQATSVS N +MESVEGNMCASA
Sbjct: 601  VHNELGVDGRNPLMTEASTAVGDKFQSKAETNSLKSNGQATSVSSNDEMESVEGNMCASA 660

Query: 661  TGVVRVQSRKKAEMFLVRTDGFSCAREKVTESSLAFTHPSTQQQMLMWKSTPKTVLLLKK 720
            TGVVRVQSR+KAEMFLVRTDGFSCAREKVTESSLAFTHPSTQQQMLMWKSTP+TVLLLKK
Sbjct: 661  TGVVRVQSRRKAEMFLVRTDGFSCAREKVTESSLAFTHPSTQQQMLMWKSTPRTVLLLKK 720

Query: 721  LGQELMEEAKEAASFLYHQEKMNVLVEPDVHDIFARIPGFGFVQTFYIQDTSDLHEKVDF 780
            LG+ELMEEAKE ASF+YHQEKMNVLVEPDVHDIFARIPGFGFVQTFY QDTSDLHEKVDF
Sbjct: 721  LGEELMEEAKEVASFMYHQEKMNVLVEPDVHDIFARIPGFGFVQTFYSQDTSDLHEKVDF 780

Query: 781  VACLGGDGVILHASNLFRGAVPPVVSFNLGSLGFLTSHTFESYRQDLRQVIHGNDSRDGV 840
            VACLGGDGVILHASN+FRGAVPPVVSFNLGSLGFLTSHTF+SYRQDLRQVIHGNDS DGV
Sbjct: 781  VACLGGDGVILHASNIFRGAVPPVVSFNLGSLGFLTSHTFDSYRQDLRQVIHGNDSLDGV 840

Query: 841  YITLRMRLQCEIFRNGKAIPGKLFNILNEVVVDRGSNPYLSKIECYEHDRLITKVQGDGV 900
            YITLRMRLQCEIFRNGKAIPGK+FNILNEVVVDRGSNPYLSKIECYEHDRLITKVQGDGV
Sbjct: 841  YITLRMRLQCEIFRNGKAIPGKVFNILNEVVVDRGSNPYLSKIECYEHDRLITKVQGDGV 900

Query: 901  IVATPTGSTAYSTAAGGSMVHPNVPCMLFTPICPHSLSFRPVILPDSARLELKIPEDTRS 960
            IVATPTGSTAYSTAAGGSMVHPNVPCMLFTPICPHSLSFRPVILPDSARLELKIPED RS
Sbjct: 901  IVATPTGSTAYSTAAGGSMVHPNVPCMLFTPICPHSLSFRPVILPDSARLELKIPEDARS 960

Query: 961  NAWVSFDGKRRQQLSRGDSVRISMSSHPLPTVNKSDQTGDWFRSLIRCLNWNERLDQKAL 1017
            NAWVSFDGKRRQQLSRGDSV+ISMS HPLPTVNKSDQTGDWFRSLIRCLNWNERLDQKAL
Sbjct: 961  NAWVSFDGKRRQQLSRGDSVKISMSRHPLPTVNKSDQTGDWFRSLIRCLNWNERLDQKAL 1017

BLAST of Sgr014308 vs. NCBI nr
Match: XP_022956006.1 (LOW QUALITY PROTEIN: NAD kinase 2, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 1741.9 bits (4510), Expect = 0.0e+00
Identity = 873/1020 (85.59%), Postives = 929/1020 (91.08%), Query Frame = 0

Query: 1    MVVCCFEWHLDAIAVGMNRSLPATVIHS--YLSPLRPLLPSC-NARFLGFQLQTWKRLRR 60
            M++CCF WHLDAIA+GMNRSLPATVIHS  YLSP RPLLPS  NARFLGFQL TWKR RR
Sbjct: 1    MLLCCFVWHLDAIALGMNRSLPATVIHSYPYLSPFRPLLPSSNNARFLGFQLHTWKRFRR 60

Query: 61   RLKFAVTAELSKSLSLNSGSEFQLPWIGPVPGDIAEVEAYCRIFRTAERLHSVLMDTLCN 120
            RLKFAVTAELSKS+S NS SEFQL W+GPVPGD+AEVEAYCRIFRTAERLHSVLMDTLCN
Sbjct: 61   RLKFAVTAELSKSVSFNSASEFQLSWMGPVPGDVAEVEAYCRIFRTAERLHSVLMDTLCN 120

Query: 121  PLTGECSVSYDVKPGKNPLIEDKIVSVLGCIVSLLNKGREDVLSGRSSAMNTFQVANLDA 180
            P TGECSVSYDV PG+NP+IEDKIVSVLGCIVSLLNKG+EDVLSGRSSAMN F+  NLDA
Sbjct: 121  PFTGECSVSYDVTPGENPVIEDKIVSVLGCIVSLLNKGKEDVLSGRSSAMNAFRGDNLDA 180

Query: 181  MEDDLPPLAAFRSEMKRCCESLHVALENFLTPGDDRSLDVWRKLQRLKNVCYDSGFSRGE 240
            MED+LPPLAAFRSEMKRCCESLHVALENFLTP DDRS DVWRKLQRLKNVCYDSGF RGE
Sbjct: 181  MEDNLPPLAAFRSEMKRCCESLHVALENFLTPDDDRSWDVWRKLQRLKNVCYDSGFPRGE 240

Query: 241  AYPCHTLFANWNPVYLHNFKEETLAKKSDVAFWSGGQVTEEGLKWLIERGFKTIVDLRAE 300
             YPCHTLFANWNPVYLHN KEE  AK SDV+FWSGGQ+TEEGLKWLIERGFKTIVDLRAE
Sbjct: 241  DYPCHTLFANWNPVYLHNSKEEVSAKNSDVSFWSGGQITEEGLKWLIERGFKTIVDLRAE 300

Query: 301  TVKDDFHTALLQDAIGSAKVKVIKIPVEVRTAPSVDQVEKFASLVSDGSNRLIYLHSKEG 360
            TVKD F++A L DAIGS KVKVI+IPVEVRTAP+ DQVEKFASLVSDGSN LIYLHSKEG
Sbjct: 301  TVKDIFYSASLHDAIGSDKVKVIRIPVEVRTAPTADQVEKFASLVSDGSNGLIYLHSKEG 360

Query: 361  VWRTSAMISRWRQYVTRSGSQFVSNQTI-GGDIPLRDTSPKLAHNQNGAKESLEIFTIQE 420
            +WRTSAMISRWRQY TRSGSQFVSNQTI   DIPL DTSPKL  N +GAKESLEI T+++
Sbjct: 361  LWRTSAMISRWRQYATRSGSQFVSNQTIVPADIPLGDTSPKLVQNPDGAKESLEISTVEK 420

Query: 421  TFPREEDNQSLLVESAHHSSIDVIGKTNYAETDKGSQNVNGAYNRPIPSQDLSSLRAVDS 480
            T   +E+++SLL+ESA H SI+   + N  E DK  QN NGA+N  IP+QDL+SLRA  +
Sbjct: 421  TSSCDENSRSLLLESADHCSIN---RKNNTEADKIIQNENGAFNGSIPTQDLTSLRADHN 480

Query: 481  GEEYLSQFSIETDPLKAQIPPCNIFSRKEMSNFFRTKKISPPNYFNHQMKRKEKWPLSIE 540
            GEE+LSQFS+ TDPLKAQIPPCNIFSRKEMSNFFRTKKISP NY + +MK  EK P S E
Sbjct: 481  GEEHLSQFSVATDPLKAQIPPCNIFSRKEMSNFFRTKKISPQNYLHRKMKTNEKRPPSTE 540

Query: 541  LPASRIQRFSVDDSNLKSGFVEAENFTGSASVKDSSSKTHYLSTTKMKYVSGDSHVSANP 600
            LP SR+Q+FSVD+S+LKSG VEA N  G  SVK++SS THYLSTT M+YV+GDSHVS+NP
Sbjct: 541  LPMSRVQKFSVDNSDLKSGLVEAGNTNGRPSVKETSSTTHYLSTTNMRYVNGDSHVSSNP 600

Query: 601  VLNGLGVDGRNPLTTEASTAVGGTFQSKSEKKDLKSNGQATSVSGNVDMESVEGNMCASA 660
            V N LGVDGRNPL TEASTAVG  FQSK+E   LKSNGQATSVS N +MESVEGNMCASA
Sbjct: 601  VHNELGVDGRNPLMTEASTAVGDKFQSKAETNSLKSNGQATSVSSNDEMESVEGNMCASA 660

Query: 661  TGVVRVQSRKKAEMFLVRTDGFSCAREKVTESSLAFTHPSTQQQMLMWKSTPKTVLLLKK 720
            TGVVRVQSR+KAEMFLVRTDGFSCAREKVTESSLAFTHPSTQQQMLMWKSTP+TVLLLKK
Sbjct: 661  TGVVRVQSRRKAEMFLVRTDGFSCAREKVTESSLAFTHPSTQQQMLMWKSTPRTVLLLKK 720

Query: 721  LGQELMEEAKEAASFLYHQEKMNVLVEPDVHDIFARIPGFGFVQTFYIQDTSDLHEKVDF 780
            LG+ELMEEAKE ASF+YHQEKMNVLVEPDVHDIFARIPGFGFVQTFY QDTSDLHEKVDF
Sbjct: 721  LGEELMEEAKEVASFMYHQEKMNVLVEPDVHDIFARIPGFGFVQTFYSQDTSDLHEKVDF 780

Query: 781  VACLGGDGVILHASNLFRGAVPPVVSFNLGSLGFLTSHTFESYRQDLRQVIHGNDSRDGV 840
            VACLGGDGVILHASN+FRGAVPPVVSFNLGSLGFLTSHTF+SYRQDLRQVIHGNDS DGV
Sbjct: 781  VACLGGDGVILHASNIFRGAVPPVVSFNLGSLGFLTSHTFDSYRQDLRQVIHGNDSLDGV 840

Query: 841  YITLRMRLQCEIFRNGKAIPGKLFNILNEVVVDRGSNPYLSKIECYEHDRLITKVQGDGV 900
            YITLRMRLQCEIFRNGKAIPGK+FNILNEVVVDRGSNPYLSKIECYEHDRLITKVQGDGV
Sbjct: 841  YITLRMRLQCEIFRNGKAIPGKVFNILNEVVVDRGSNPYLSKIECYEHDRLITKVQGDGV 900

Query: 901  IVATPTGSTAYSTAAGGSMVHPNVPCMLFTPICPHSLSFRPVILPDSARLELKIPEDTRS 960
            IVATPTGSTAYSTAAGGSMVHPNVPCMLFTPICPHSLSFRPVILPDSARLELKIPED RS
Sbjct: 901  IVATPTGSTAYSTAAGGSMVHPNVPCMLFTPICPHSLSFRPVILPDSARLELKIPEDARS 960

Query: 961  NAWVSFDGKRRQQLSRGDSVRISMSSHPLPTVNKSDQTGDWFRSLIRCLNWNERLDQKAL 1017
            NAWVSFDGKRRQQLSRGDSV+ISMS HPLPTVNKSDQTGDWFRSLIRCLNWNERLDQKAL
Sbjct: 961  NAWVSFDGKRRQQLSRGDSVKISMSRHPLPTVNKSDQTGDWFRSLIRCLNWNERLDQKAL 1017

BLAST of Sgr014308 vs. NCBI nr
Match: XP_022990760.1 (LOW QUALITY PROTEIN: NAD kinase 2, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 1741.5 bits (4509), Expect = 0.0e+00
Identity = 874/1020 (85.69%), Postives = 929/1020 (91.08%), Query Frame = 0

Query: 1    MVVCCFEWHLDAIAVGMNRSLPATVIHS--YLSPLRPLLPSC-NARFLGFQLQTWKRLRR 60
            M++CCF WHLDAIAVGMNRSLPATVIHS  YLSP R LLPS  NARFLGFQL TW+R RR
Sbjct: 1    MLLCCFVWHLDAIAVGMNRSLPATVIHSYPYLSPFRLLLPSSNNARFLGFQLHTWRRFRR 60

Query: 61   RLKFAVTAELSKSLSLNSGSEFQLPWIGPVPGDIAEVEAYCRIFRTAERLHSVLMDTLCN 120
            RLKFAVTAELSKS+S NS SEFQL W+GPVPGDIAEVEAYCRIFRTAERLHSVLMDTLCN
Sbjct: 61   RLKFAVTAELSKSVSFNSASEFQLSWMGPVPGDIAEVEAYCRIFRTAERLHSVLMDTLCN 120

Query: 121  PLTGECSVSYDVKPGKNPLIEDKIVSVLGCIVSLLNKGREDVLSGRSSAMNTFQVANLDA 180
            P TGECSVSYDV PG+NP+IEDKIVSVLGCIVSLLNKG+EDVLSGRSSAMN F+  NLDA
Sbjct: 121  PFTGECSVSYDVTPGENPVIEDKIVSVLGCIVSLLNKGKEDVLSGRSSAMNAFRGDNLDA 180

Query: 181  MEDDLPPLAAFRSEMKRCCESLHVALENFLTPGDDRSLDVWRKLQRLKNVCYDSGFSRGE 240
            MED+LPPLAAFRSEMKRCCESLHVALENFLTP DDRS DVWRKLQRLKNVCYDSGF RGE
Sbjct: 181  MEDNLPPLAAFRSEMKRCCESLHVALENFLTPDDDRSWDVWRKLQRLKNVCYDSGFPRGE 240

Query: 241  AYPCHTLFANWNPVYLHNFKEETLAKKSDVAFWSGGQVTEEGLKWLIERGFKTIVDLRAE 300
             YPCHTLFANWNPVYLHN KEE  AK SDV+FWSGGQ+TEEGLKWLIERGFKTIVDLRAE
Sbjct: 241  DYPCHTLFANWNPVYLHNSKEEVSAKNSDVSFWSGGQITEEGLKWLIERGFKTIVDLRAE 300

Query: 301  TVKDDFHTALLQDAIGSAKVKVIKIPVEVRTAPSVDQVEKFASLVSDGSNRLIYLHSKEG 360
            TVKD F++A L DAIGS KVKVI+IPVEVRTAP+ DQVEKFASLVSDGSN L+YLHSKEG
Sbjct: 301  TVKDIFYSASLHDAIGSDKVKVIRIPVEVRTAPTADQVEKFASLVSDGSNGLLYLHSKEG 360

Query: 361  VWRTSAMISRWRQYVTRSGSQFVSNQTI-GGDIPLRDTSPKLAHNQNGAKESLEIFTIQE 420
            VWRTSAMISRWRQY TRSGSQFVSNQTI   DIPL DTSPKL  N NGAKESLEI T+++
Sbjct: 361  VWRTSAMISRWRQYATRSGSQFVSNQTIVPADIPLGDTSPKLVQNPNGAKESLEISTVEK 420

Query: 421  TFPREEDNQSLLVESAHHSSIDVIGKTNYAETDKGSQNVNGAYNRPIPSQDLSSLRAVDS 480
            T   +E++QSLL+ESA H SI+   + N AE DK  QN NGA+N  IP+QDL+SLRA  +
Sbjct: 421  TSSCDENSQSLLLESADHRSIN---RKNNAEADKIFQNENGAFNGAIPTQDLTSLRADHN 480

Query: 481  GEEYLSQFSIETDPLKAQIPPCNIFSRKEMSNFFRTKKISPPNYFNHQMKRKEKWPLSIE 540
            GEE+LSQFS+ TDPLKAQIPPCNIFSRKEMSNFFRTKKISP NY + +MK  EK P S E
Sbjct: 481  GEEHLSQFSVATDPLKAQIPPCNIFSRKEMSNFFRTKKISPQNYLHRKMKTNEKRPPSTE 540

Query: 541  LPASRIQRFSVDDSNLKSGFVEAENFTGSASVKDSSSKTHYLSTTKMKYVSGDSHVSANP 600
            LP SR+Q+FSVD+S+LKSG VEA N  G  +VK++SS THYLSTT M+YV+GDSHVS+NP
Sbjct: 541  LPMSRVQKFSVDNSDLKSGLVEAGNTNGRPTVKETSSSTHYLSTTNMRYVNGDSHVSSNP 600

Query: 601  VLNGLGVDGRNPLTTEASTAVGGTFQSKSEKKDLKSNGQATSVSGNVDMESVEGNMCASA 660
            V N LGVDGRNPL TEASTAVG  FQSK+E   LKSNGQATSVS N +M+SVEGNMCASA
Sbjct: 601  VHNELGVDGRNPLMTEASTAVGDKFQSKAETNSLKSNGQATSVSSNDEMKSVEGNMCASA 660

Query: 661  TGVVRVQSRKKAEMFLVRTDGFSCAREKVTESSLAFTHPSTQQQMLMWKSTPKTVLLLKK 720
            TGVVRVQSR+KAEMFLVRTDGFSCAREKVTESSLAFTHPSTQQQMLMWKSTP+TVLLLKK
Sbjct: 661  TGVVRVQSRRKAEMFLVRTDGFSCAREKVTESSLAFTHPSTQQQMLMWKSTPRTVLLLKK 720

Query: 721  LGQELMEEAKEAASFLYHQEKMNVLVEPDVHDIFARIPGFGFVQTFYIQDTSDLHEKVDF 780
            LG+ELMEEAKE ASF+YHQEKMNVLVEPDVHDIFARIPGFGFVQTFY QDTSDLHEKVDF
Sbjct: 721  LGEELMEEAKEVASFMYHQEKMNVLVEPDVHDIFARIPGFGFVQTFYSQDTSDLHEKVDF 780

Query: 781  VACLGGDGVILHASNLFRGAVPPVVSFNLGSLGFLTSHTFESYRQDLRQVIHGNDSRDGV 840
            VACLGGDGVILHASN+FRGAVPPVVSFNLGSLGFLTSHTF+SYRQDLRQVIHGNDS DGV
Sbjct: 781  VACLGGDGVILHASNIFRGAVPPVVSFNLGSLGFLTSHTFDSYRQDLRQVIHGNDSLDGV 840

Query: 841  YITLRMRLQCEIFRNGKAIPGKLFNILNEVVVDRGSNPYLSKIECYEHDRLITKVQGDGV 900
            YITLRMRLQCEIFRNGKAIPGK+FNILNEVVVDRGSNPYLSKIECYEHDRLITKVQGDGV
Sbjct: 841  YITLRMRLQCEIFRNGKAIPGKVFNILNEVVVDRGSNPYLSKIECYEHDRLITKVQGDGV 900

Query: 901  IVATPTGSTAYSTAAGGSMVHPNVPCMLFTPICPHSLSFRPVILPDSARLELKIPEDTRS 960
            IVATPTGSTAYSTAAGGSMVHPNVPCMLFTPICPHSLSFRPVILPDSARLELKIPED RS
Sbjct: 901  IVATPTGSTAYSTAAGGSMVHPNVPCMLFTPICPHSLSFRPVILPDSARLELKIPEDARS 960

Query: 961  NAWVSFDGKRRQQLSRGDSVRISMSSHPLPTVNKSDQTGDWFRSLIRCLNWNERLDQKAL 1017
            NAWVSFDGKRRQQLSRGDSV+ISMS HPLPTVNKSDQTGDWFRSLIRCLNWNERLDQKAL
Sbjct: 961  NAWVSFDGKRRQQLSRGDSVKISMSRHPLPTVNKSDQTGDWFRSLIRCLNWNERLDQKAL 1017

BLAST of Sgr014308 vs. ExPASy Swiss-Prot
Match: Q9C5W3 (NAD kinase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NADK2 PE=1 SV=1)

HSP 1 Score: 1197.6 bits (3097), Expect = 0.0e+00
Identity = 636/1022 (62.23%), Postives = 748/1022 (73.19%), Query Frame = 0

Query: 17   MNRSLPATVIHSYLSPLRPLLPSCNARFLGFQLQTWKR---LRRRLKFAVTAELSKSLSL 76
            M+R  PAT I S L     L  S + R + F  +  +     +RRL+F + A+LS++ S 
Sbjct: 13   MSRLSPATGISSRLRFSIGL--SSDGRLIPFGFRFRRNDVPFKRRLRFVIRAQLSEAFSP 72

Query: 77   NSGSEFQ---------LPWIGPVPGDIAEVEAYCRIFRTAERLHSVLMDTLCNPLTGECS 136
            + G + Q         LPWIGPVPGDIAEVEAYCRIFR+AERLH  LM+TLCNP+TGEC 
Sbjct: 73   DLGLDSQAVKSRDTSNLPWIGPVPGDIAEVEAYCRIFRSAERLHGALMETLCNPVTGECR 132

Query: 137  VSYDVKPGKNPLIEDKIVSVLGCIVSLLNKGREDVLSGRSSAMNTFQVANLDAMEDDLPP 196
            V YD  P + PL+EDKIVSVLGCI+SLLNKGR+++LSGRSS+MN+F + ++   E+ LPP
Sbjct: 133  VPYDFSPEEKPLLEDKIVSVLGCILSLLNKGRKEILSGRSSSMNSFNLDDVGVAEESLPP 192

Query: 197  LAAFRSEMKRCCESLHVALENFLTPGDDRSLDVWRKLQRLKNVCYDSGFSRGEAYPCHTL 256
            LA FR EMKRCCESLH+ALEN+LTP D+RS  VWRKLQ+LKNVCYD+GF R + YPC TL
Sbjct: 193  LAVFRGEMKRCCESLHIALENYLTPDDERSGIVWRKLQKLKNVCYDAGFPRSDNYPCQTL 252

Query: 257  FANWNPVYLHNFKEETLAKKSDVAFWSGGQVTEEGLKWLIERGFKTIVDLRAETVKDDFH 316
            FANW+P+Y  N KE+  + +S++AFW GGQVT+EGLKWLIE GFKTIVDLRAE VKD F+
Sbjct: 253  FANWDPIYSSNTKEDIDSYESEIAFWRGGQVTQEGLKWLIENGFKTIVDLRAEIVKDTFY 312

Query: 317  TALLQDAIGSAKVKVIKIPVEVRTAPSVDQVEKFASLVSDGSNRLIYLHSKEGVWRTSAM 376
               L DAI   K+ V++IP++VR AP  +QVE FAS+VSD S R IY+HSKEGVWRTSAM
Sbjct: 313  QTALDDAISLGKITVVQIPIDVRMAPKAEQVELFASIVSDSSKRPIYVHSKEGVWRTSAM 372

Query: 377  ISRWRQYVTRSGSQFVSNQTIGGDIPLRDTSPKLAHNQNGAKESLEIFTIQETFPREEDN 436
            +SRW+QY+TR          I  +IP                       + E   R E +
Sbjct: 373  VSRWKQYMTR---------PITKEIP-----------------------VSEESKRREVS 432

Query: 437  QSLLVESAHHSSIDVIGKTNYAETDKGSQNVNGAYNRPIPSQDLSSLRAVDSGEEYLSQF 496
            ++ L  +A  S   V  +    +TDK S+ +N   +R   SQ   S R    G+   S+F
Sbjct: 433  ETKLGSNAVVSGKGVPDE----QTDKVSE-INEVDSRSASSQSKESGRF--EGDTSASEF 492

Query: 497  SIETDPLKAQIPPCNIFSRKEMSNFFRTKKISPPNYFNHQMKRKEKWPLSIELPASRIQR 556
            ++ +DPLK+Q+PP NIFSRKEMS F ++K I+P  Y  +  K     P            
Sbjct: 493  NMVSDPLKSQVPPGNIFSRKEMSKFLKSKSIAPAGYLTNPSKILGTVPTPQFSYTGVTNG 552

Query: 557  FSVDDSNLKSGFVEAENFTGSASVKDSSSKTHYLSTTKMKYVSGDSHVSANPVLNGLGVD 616
              + D +      E  N  G+     S S    L     K+ +G+ H S N   N    D
Sbjct: 553  NQIVDKDSIRRLAETGNSNGTLLPTSSQS----LDFGNGKFSNGNVHASDN--TNKSISD 612

Query: 617  GRN----------PLTTEASTAVGGTFQSKSEKKDLKSNGQATSVSGNVDMESVEGNMCA 676
             R           P +   S AVG    S  E +  ++N  ++S S + +  ++EGNMCA
Sbjct: 613  NRGNGFSAAPIAVPPSDNLSRAVGS--HSVRESQTQRNNSGSSSDSSDDEAGAIEGNMCA 672

Query: 677  SATGVVRVQSRKKAEMFLVRTDGFSCAREKVTESSLAFTHPSTQQQMLMWKSTPKTVLLL 736
            SATGVVRVQSRKKAEMFLVRTDG SC REKVTESSLAFTHPSTQQQML+WK+TPKTVLLL
Sbjct: 673  SATGVVRVQSRKKAEMFLVRTDGVSCTREKVTESSLAFTHPSTQQQMLLWKTTPKTVLLL 732

Query: 737  KKLGQELMEEAKEAASFLYHQEKMNVLVEPDVHDIFARIPGFGFVQTFYIQDTSDLHEKV 796
            KKLGQELMEEAKEAASFLYHQE MNVLVEP+VHD+FARIPGFGFVQTFYIQDTSDLHE+V
Sbjct: 733  KKLGQELMEEAKEAASFLYHQENMNVLVEPEVHDVFARIPGFGFVQTFYIQDTSDLHERV 792

Query: 797  DFVACLGGDGVILHASNLFRGAVPPVVSFNLGSLGFLTSHTFESYRQDLRQVIHGNDSRD 856
            DFVACLGGDGVILHASNLF+GAVPPVVSFNLGSLGFLTSH FE +RQDL++VIHGN++ D
Sbjct: 793  DFVACLGGDGVILHASNLFKGAVPPVVSFNLGSLGFLTSHPFEDFRQDLKRVIHGNNTLD 852

Query: 857  GVYITLRMRLQCEIFRNGKAIPGKLFNILNEVVVDRGSNPYLSKIECYEHDRLITKVQGD 916
            GVYITLRMRL+CEI+R GKA+PGK+F++LNE+VVDRGSNPYLSKIECYEHDRLITKVQGD
Sbjct: 853  GVYITLRMRLRCEIYRKGKAMPGKVFDVLNEIVVDRGSNPYLSKIECYEHDRLITKVQGD 912

Query: 917  GVIVATPTGSTAYSTAAGGSMVHPNVPCMLFTPICPHSLSFRPVILPDSARLELKIPEDT 976
            GVIVATPTGSTAYSTAAGGSMVHPNVPCMLFTPICPHSLSFRPVILPDSA+LELKIP+D 
Sbjct: 913  GVIVATPTGSTAYSTAAGGSMVHPNVPCMLFTPICPHSLSFRPVILPDSAKLELKIPDDA 972

Query: 977  RSNAWVSFDGKRRQQLSRGDSVRISMSSHPLPTVNKSDQTGDWFRSLIRCLNWNERLDQK 1017
            RSNAWVSFDGKRRQQLSRGDSVRI MS HPLPTVNKSDQTGDWFRSLIRCLNWNERLDQK
Sbjct: 973  RSNAWVSFDGKRRQQLSRGDSVRIYMSQHPLPTVNKSDQTGDWFRSLIRCLNWNERLDQK 985

BLAST of Sgr014308 vs. ExPASy Swiss-Prot
Match: Q53NI2 (Probable NAD kinase 2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os11g0191400 PE=2 SV=1)

HSP 1 Score: 1103.2 bits (2852), Expect = 0.0e+00
Identity = 566/950 (59.58%), Postives = 701/950 (73.79%), Query Frame = 0

Query: 80   QLPWIGPVPGDIAEVEAYCRIFRTAERLHSVLMDTLCNPLTGECSVSYDVKPGKNPLIED 139
            QL W+GPVPGDIAE+EAYCRIFR AE+LH+ +M  LC+P TGEC V YDV+    P++ED
Sbjct: 71   QLLWVGPVPGDIAEIEAYCRIFRAAEQLHTAVMSALCDPETGECPVRYDVQTEDLPVLED 130

Query: 140  KIVSVLGCIVSLLNKGREDVLSGRSSAMNTFQVANLDAMEDDLPPLAAFRSEMKRCCESL 199
            K+ +VLGC+++LLN+GR++VLSGRS   + FQ  + D+  D +PPLA FR ++KRCCES+
Sbjct: 131  KVAAVLGCMLALLNRGRKEVLSGRSGVASAFQ-GSEDSTMDKIPPLALFRGDLKRCCESM 190

Query: 200  HVALENFLTPGDDRSLDVWRKLQRLKNVCYDSGFSRGEAYPCHTLFANWNPVYLHNFKEE 259
             VAL ++L P + R LD+WRKLQRLKN CYD+GF R + +PC TLFANW PVY     ++
Sbjct: 191  QVALASYLVPSEARGLDIWRKLQRLKNACYDAGFPRADGHPCPTLFANWFPVYFSTVPDD 250

Query: 260  TLAKKSDVAFWSGGQVTEEGLKWLIERGFKTIVDLRAETVKDDFHTALLQDAIGSAKVKV 319
            +L+ + +VAFW GGQV+EEGL+WL+ +GFKTIVDLR E VKDD + + + +A+   K++V
Sbjct: 251  SLSDELEVAFWRGGQVSEEGLEWLLLKGFKTIVDLREEDVKDDLYLSAIHEAVSLGKIEV 310

Query: 320  IKIPVEVRTAPSVDQVEKFASLVSDGSNRLIYLHSKEGVWRTSAMISRWRQYVTRSGSQF 379
            + +PVE+ TAPS +QV++FA +VSD + + IYLHS+EG+ RTSAM+SRW+QYVTR+    
Sbjct: 311  VNLPVEIGTAPSAEQVQRFAEIVSDSAKKPIYLHSQEGISRTSAMVSRWKQYVTRAERLA 370

Query: 380  VSNQTIGGD--IPLRDTSPKLAHNQNGAKESLEIFT-IQETFPREEDNQSLLVESAHHSS 439
              N+++ G+      D + +L ++   + E  E  T ++     E +   + +E+A H+ 
Sbjct: 371  TQNRSLNGNGKHVRNDQTEQLTNSPGFSSEGSENGTPLESDRTMEGETCDIDIETARHN- 430

Query: 440  IDVIGKTNYAETDKGSQNVNGAYNRPIPSQDLSSLRAVDSGEEYLSQFSIETDPLKAQIP 499
               +  TN   +++ ++             +L   R      E  S F +E++PLKAQ P
Sbjct: 431  ---LEITNSLPSEQSTE-----------QGELHGTRT-----ELQSNFRLESNPLKAQFP 490

Query: 500  PCNIFSRKEMSNFFRTKKISPPNYFNHQMK------RKEKWPLSIELPAS---RIQRFSV 559
             C++FS+K M++FFR+KK+ P +  N + +       + K  LS E   +       F V
Sbjct: 491  SCDVFSKKGMTDFFRSKKVYPKSVLNPRRRSNSLLVSRRKQSLSAEQNGAIDYEAAEFKV 550

Query: 560  DDSNLKSGFVEAENFTGSASVKDSSSKTHYLSTTKMKYVSGDSHVSANP-VLNGLGVDGR 619
              S+  + F      + ++ + +     +  ST+        S V+ +P   +    +G 
Sbjct: 551  LKSSNGASFDNDYILSVASGITNGKPSNNGASTSVEDREMETSVVTVDPRTSDTSNSNGN 610

Query: 620  NPLTTEASTAVGGTFQSKSEKKDLKSNGQATSVSGNVDMESVEGNMCASATGVVRVQSRK 679
             PL ++ S    G+   + EK D                  V+GNMCASATGVVR+QSR+
Sbjct: 611  APLGSQKSAERNGSLYVEREKSD-----------------HVDGNMCASATGVVRLQSRR 670

Query: 680  KAEMFLVRTDGFSCAREKVTESSLAFTHPSTQQQMLMWKSTPKTVLLLKKLGQELMEEAK 739
            KAEMFLVRTDGFSC REKVTESSLAFTHPSTQQQMLMWKS PKTVLLLKKLG ELMEEAK
Sbjct: 671  KAEMFLVRTDGFSCTREKVTESSLAFTHPSTQQQMLMWKSPPKTVLLLKKLGDELMEEAK 730

Query: 740  EAASFLYHQEKMNVLVEPDVHDIFARIPGFGFVQTFYIQDTSDLHEKVDFVACLGGDGVI 799
            E ASFL+HQEKMNVLVEPDVHDIFARIPG+GFVQTFY QDTSDLHE+VDFVACLGGDGVI
Sbjct: 731  EVASFLHHQEKMNVLVEPDVHDIFARIPGYGFVQTFYTQDTSDLHERVDFVACLGGDGVI 790

Query: 800  LHASNLFRGAVPPVVSFNLGSLGFLTSHTFESYRQDLRQVIHGNDSRDGVYITLRMRLQC 859
            LHASNLFR +VPPVVSFNLGSLGFLTSH FE +RQDLR VIHGN++  GVYITLRMRL+C
Sbjct: 791  LHASNLFRTSVPPVVSFNLGSLGFLTSHNFEGFRQDLRAVIHGNNTL-GVYITLRMRLRC 850

Query: 860  EIFRNGKAIPGKLFNILNEVVVDRGSNPYLSKIECYEHDRLITKVQGDGVIVATPTGSTA 919
            EIFRNGKA+PGK+F++LNEVVVDRGSNPYLSKIECYEH+ LITKVQGDGVIVATPTGSTA
Sbjct: 851  EIFRNGKAMPGKVFDVLNEVVVDRGSNPYLSKIECYEHNHLITKVQGDGVIVATPTGSTA 910

Query: 920  YSTAAGGSMVHPNVPCMLFTPICPHSLSFRPVILPDSARLELKIPEDTRSNAWVSFDGKR 979
            YSTAAGGSMVHPNVPCMLFTPICPHSLSFRPVILPDSARLELKIP+D RSNAWVSFDGKR
Sbjct: 911  YSTAAGGSMVHPNVPCMLFTPICPHSLSFRPVILPDSARLELKIPDDARSNAWVSFDGKR 970

Query: 980  RQQLSRGDSVRISMSSHPLPTVNKSDQTGDWFRSLIRCLNWNERLDQKAL 1017
            RQQLSRGDSV+ISMS HPLPTVNKSDQTGDWFRSLIRCLNWNERLDQKAL
Sbjct: 971  RQQLSRGDSVQISMSQHPLPTVNKSDQTGDWFRSLIRCLNWNERLDQKAL 981

BLAST of Sgr014308 vs. ExPASy Swiss-Prot
Match: P58058 (NAD kinase OS=Mus musculus OX=10090 GN=Nadk PE=1 SV=2)

HSP 1 Score: 292.7 bits (748), Expect = 1.6e-77
Identity = 151/339 (44.54%), Postives = 215/339 (63.42%), Query Frame = 0

Query: 700  QMLMWKSTPKTVLLLKKL-GQELMEEAKEAASFLYHQEKMNVLVEPDVHDIFARI--PGF 759
            Q L W  +PK+VL++KK+    L++  KE   +L  +  M V VE  V +  A +    F
Sbjct: 96   QRLTWNKSPKSVLVIKKIRDASLLQPFKELCIYLMEENNMIVYVEKKVLEDPAIVSDENF 155

Query: 760  GFVQTFYI---QDTSDLHEKVDFVACLGGDGVILHASNLFRGAVPPVVSFNLGSLGFLTS 819
            G V+  +    +D  D+  ++DF+ CLGGDG +L+AS+LF+G+VPPV++F+LGSLGFLT 
Sbjct: 156  GPVKKKFCTFREDYDDISNQIDFIICLGGDGTLLYASSLFQGSVPPVMAFHLGSLGFLTP 215

Query: 820  HTFESYRQDLRQVIHGNDSRDGVYITLRMRLQCEIFR---------------NGKAIPG- 879
              FE+++  + QVI GN +     + LR RL+  + +               NG    G 
Sbjct: 216  FNFENFQSQVNQVIEGNAA-----VILRSRLKVRVVKEPRDKKTAIHNGLSENGLDTEGG 275

Query: 880  ---KLFNILNEVVVDRGSNPYLSKIECYEHDRLITKVQGDGVIVATPTGSTAYSTAAGGS 939
                 + +LNEVV+DRG + YLS ++ Y    LIT VQGDGVIV+TPTGSTAY+ AAG S
Sbjct: 276  KQAMQYQVLNEVVIDRGPSSYLSNVDVYLDGHLITTVQGDGVIVSTPTGSTAYAAAAGAS 335

Query: 940  MVHPNVPCMLFTPICPHSLSFRPVILPDSARLELKIPEDTRSNAWVSFDGKRRQQLSRGD 999
            MVHPNVP ++ TPICPHSLSFRP+++P    L++ +  + R+ AWVSFDG++RQ++  GD
Sbjct: 336  MVHPNVPAIMVTPICPHSLSFRPIVVPAGVELKIMLSPEARNTAWVSFDGRKRQEIRHGD 395

Query: 1000 SVRISMSSHPLPTVNKSDQTGDWFRSLIRCLNWNERLDQ 1014
            S+ I+ S +PLP++   D   DWF SL +CL+WN R  Q
Sbjct: 396  SISITTSCYPLPSICVCDPVSDWFESLAQCLHWNVRKKQ 429

BLAST of Sgr014308 vs. ExPASy Swiss-Prot
Match: O95544 (NAD kinase OS=Homo sapiens OX=9606 GN=NADK PE=1 SV=1)

HSP 1 Score: 290.8 bits (743), Expect = 5.9e-77
Identity = 151/344 (43.90%), Postives = 216/344 (62.79%), Query Frame = 0

Query: 700  QMLMWKSTPKTVLLLKKL-GQELMEEAKEAASFLYHQEKMNVLVEPDVHD--IFARIPGF 759
            Q L W  +PK+VL++KK+    L++  KE  + L  +E M V VE  V +    A    F
Sbjct: 96   QRLTWNKSPKSVLVIKKMRDASLLQPFKELCTHLM-EENMIVYVEKKVLEDPAIASDESF 155

Query: 760  GFVQTFYI---QDTSDLHEKVDFVACLGGDGVILHASNLFRGAVPPVVSFNLGSLGFLTS 819
            G V+  +    +D  D+  ++DF+ CLGGDG +L+AS+LF+G+VPPV++F+LGSLGFLT 
Sbjct: 156  GAVKKKFCTFREDYDDISNQIDFIICLGGDGTLLYASSLFQGSVPPVMAFHLGSLGFLTP 215

Query: 820  HTFESYRQDLRQVIHGNDSRDGVYITLRMRLQCEIFR---------------NGKAIPG- 879
             +FE+++  + QVI GN +     + LR RL+  + +               NG    G 
Sbjct: 216  FSFENFQSQVTQVIEGNAA-----VVLRSRLKVRVVKELRGKKTAVHNGLGENGSQAAGL 275

Query: 880  --------KLFNILNEVVVDRGSNPYLSKIECYEHDRLITKVQGDGVIVATPTGSTAYST 939
                      + +LNEVV+DRG + YLS ++ Y    LIT VQGDGVIV+TPTGSTAY+ 
Sbjct: 276  DMDVGKQAMQYQVLNEVVIDRGPSSYLSNVDVYLDGHLITTVQGDGVIVSTPTGSTAYAA 335

Query: 940  AAGGSMVHPNVPCMLFTPICPHSLSFRPVILPDSARLELKIPEDTRSNAWVSFDGKRRQQ 999
            AAG SM+HPNVP ++ TPICPHSLSFRP+++P    L++ +  + R+ AWVSFDG++RQ+
Sbjct: 336  AAGASMIHPNVPAIMITPICPHSLSFRPIVVPAGVELKIMLSPEARNTAWVSFDGRKRQE 395

Query: 1000 LSRGDSVRISMSSHPLPTVNKSDQTGDWFRSLIRCLNWNERLDQ 1014
            +  GDS+ I+ S +PLP++   D   DWF SL +CL+WN R  Q
Sbjct: 396  IRHGDSISITTSCYPLPSICVRDPVSDWFESLAQCLHWNVRKKQ 433

BLAST of Sgr014308 vs. ExPASy Swiss-Prot
Match: Q56YN3 (NAD(H) kinase 1 OS=Arabidopsis thaliana OX=3702 GN=NADK1 PE=1 SV=2)

HSP 1 Score: 277.3 bits (708), Expect = 6.8e-73
Identity = 150/327 (45.87%), Postives = 215/327 (65.75%), Query Frame = 0

Query: 696  STQQQMLMWKSTPKTVLLLKKLGQELMEE-AKEAASFLYHQEKMNVLVEPDV-HDIFARI 755
            S++Q  L W+S P+TVL++ K     +   + +   +L  Q+ +N+ VEP V  ++ +  
Sbjct: 201  SSKQISLTWESDPQTVLIITKPNSTSVRVLSVDMVRWLRTQKGLNIYVEPRVKEELLSES 260

Query: 756  PGFGFVQTFY-IQDTSDLHEKVDFVACLGGDGVILHASNLFRGAVPPVVSFNLGSLGFLT 815
              F FVQT+   ++ S LH KVD +  LGGDG +L A+++F+G VPP+V F++GSLGF+T
Sbjct: 261  SSFNFVQTWEDDKEISLLHTKVDLLITLGGDGTVLWAASMFKGPVPPIVPFSMGSLGFMT 320

Query: 816  SHTFESYRQDLRQVIHGNDSRDGVYITLRMRLQCEIFRNGKAI----PGKLFNILNEVVV 875
                E YR  L  ++ G      + ITLR RLQC I R+ KA     P +   +LNEV +
Sbjct: 321  PFHSEQYRDCLEAILKG-----PISITLRHRLQCHIIRD-KATHEYEPEETMLVLNEVTI 380

Query: 876  DRGSNPYLSKIECYEHDRLITKVQGDGVIVATPTGSTAYSTAAGGSMVHPNVPCMLFTPI 935
            DRG + YL+ +ECY  +  +T VQGDG+I++T +GSTAYS AAGGSMVHP VP +LFTPI
Sbjct: 381  DRGISSYLTNLECYCDNSFVTCVQGDGLILSTTSGSTAYSLAAGGSMVHPQVPGILFTPI 440

Query: 936  CPHSLSFRPVILPDSARLELKIPEDTRSNAWVSFDGKRRQQLSRGDSVRISMSSHPLPTV 995
            CPHSLSFRP+ILP+   + +++P ++RS+AWVSFDGK R+QL  GD++  SM+  P+ T 
Sbjct: 441  CPHSLSFRPLILPEHVTVRVQVPFNSRSSAWVSFDGKDRKQLEAGDALVCSMAPWPVSTA 500

Query: 996  NKSDQTGDWFRSLIRCLNWNERLDQKA 1016
             + + T D+ RS+   L+WN R  Q A
Sbjct: 501  CQVESTNDFLRSIHDGLHWNLRKTQSA 521

BLAST of Sgr014308 vs. ExPASy TrEMBL
Match: A0A6J1CJG2 (NAD kinase 2, chloroplastic isoform X1 OS=Momordica charantia OX=3673 GN=LOC111011988 PE=3 SV=1)

HSP 1 Score: 1859.7 bits (4816), Expect = 0.0e+00
Identity = 924/1016 (90.94%), Postives = 957/1016 (94.19%), Query Frame = 0

Query: 1    MVVCCFEWHLDAIAVGMNRSLPATVIHSYLSPLRPLLPSCNARFLGFQLQTWKRLRRRLK 60
            MVVCCFEW LDAIAVGMNRSLPATVIHSYLSP RPLLPSCNARFLGFQLQTWKRLRRRLK
Sbjct: 1    MVVCCFEWQLDAIAVGMNRSLPATVIHSYLSPFRPLLPSCNARFLGFQLQTWKRLRRRLK 60

Query: 61   FAVTAELSKSLSLNSGSEFQLPWIGPVPGDIAEVEAYCRIFRTAERLHSVLMDTLCNPLT 120
            FAVTAELSKS+SLNSGSEFQLPWIGPVPGDIAEVEAYCRIFRTAERLHSVLMDTLCNPLT
Sbjct: 61   FAVTAELSKSVSLNSGSEFQLPWIGPVPGDIAEVEAYCRIFRTAERLHSVLMDTLCNPLT 120

Query: 121  GECSVSYDVKPGKNPLIEDKIVSVLGCIVSLLNKGREDVLSGRSSAMNTFQVANLDAMED 180
            GECSVSYD+ PG+NP+IEDKIVSVLGC+VSLLNKGREDVLSGRS+AMN FQ ANLD  ED
Sbjct: 121  GECSVSYDLTPGENPVIEDKIVSVLGCLVSLLNKGREDVLSGRSAAMNCFQAANLDVTED 180

Query: 181  DLPPLAAFRSEMKRCCESLHVALENFLTPGDDRSLDVWRKLQRLKNVCYDSGFSRGEAYP 240
             LPPLAAFRSEMKRCCESLHVALENFLTPGDDRSLDVW KLQRLKNVCYDSGF+RGE YP
Sbjct: 181  SLPPLAAFRSEMKRCCESLHVALENFLTPGDDRSLDVWGKLQRLKNVCYDSGFTRGEDYP 240

Query: 241  CHTLFANWNPVYLHNFKEETLAKKSDVAFWSGGQVTEEGLKWLIERGFKTIVDLRAETVK 300
            CHTLFANWNPVYLHNFKEETL K SDVAFWSGGQVTEEGLKWLIERGFKTIVDLRAETVK
Sbjct: 241  CHTLFANWNPVYLHNFKEETLEKNSDVAFWSGGQVTEEGLKWLIERGFKTIVDLRAETVK 300

Query: 301  DDFHTALLQDAIGSAKVKVIKIPVEVRTAPSVDQVEKFASLVSDGSNRLIYLHSKEGVWR 360
            DDF+ A L DAI SAKVKVIKIPVEVRTAP+VDQVEKF+SLVSDGSNR IYLHSKEGVWR
Sbjct: 301  DDFYNAFLHDAICSAKVKVIKIPVEVRTAPTVDQVEKFSSLVSDGSNRPIYLHSKEGVWR 360

Query: 361  TSAMISRWRQYVTRSGSQFVSNQTIGGDIPLRDTSPKLAHNQNGAKESLEIFTIQETFPR 420
            TSAMISRWRQYVTRS SQ VSNQTI GDIPLRDTS KL HNQNGAKESLEI  I+ETF  
Sbjct: 361  TSAMISRWRQYVTRSESQLVSNQTIAGDIPLRDTSSKLEHNQNGAKESLEISKIEETFTC 420

Query: 421  EEDNQSLLVESAHHSSIDVIGKTNYAETDKGSQNVNGAYNRPIPSQDLSSLRAVDSGEEY 480
            E+DNQSLLVESA H SID+I K N A+T+  SQ VNGA++ PIP+QDL+S RAVDSGEEY
Sbjct: 421  EKDNQSLLVESARHGSIDLISKKNCAQTETESQTVNGAFHGPIPTQDLTSPRAVDSGEEY 480

Query: 481  LSQFSIETDPLKAQIPPCNIFSRKEMSNFFRTKKISPPNYFNHQMKRKEKWPLSIELPAS 540
             SQ  IETDPLKAQIPPCN+FS+KEMSNFFRTKKISP NYFNH +K++EKW LS ELPA 
Sbjct: 481  PSQSGIETDPLKAQIPPCNVFSKKEMSNFFRTKKISPQNYFNHHLKKREKWLLSTELPAR 540

Query: 541  RIQRFSVDDSNLKSGFVEAENFTGSASVKDSSSKTHYLSTTKMKYVSGDSHVSANPVLNG 600
            RIQRFSVDDSNLKSGFVEA NF GS SVKD+SS THYLSTTKMKYV+GDSHVSANPVLNG
Sbjct: 541  RIQRFSVDDSNLKSGFVEAGNFNGSPSVKDTSSNTHYLSTTKMKYVNGDSHVSANPVLNG 600

Query: 601  LGVDGRNPLTTEASTAVGGTFQSKSEKKDLKSNGQATSVSGNVDMESVEGNMCASATGVV 660
            L VDGRN +T EASTAVG  FQS+SEKK+LKSNGQA SVSGNVDMESVEGNMCASATGVV
Sbjct: 601  LEVDGRNSVTIEASTAVGSKFQSESEKKELKSNGQAISVSGNVDMESVEGNMCASATGVV 660

Query: 661  RVQSRKKAEMFLVRTDGFSCAREKVTESSLAFTHPSTQQQMLMWKSTPKTVLLLKKLGQE 720
            RVQSR+KAEMFLVRTDGFSCAREKVTESSLAFTHPSTQQQMLMWKSTPKTVLLLKKLGQE
Sbjct: 661  RVQSRRKAEMFLVRTDGFSCAREKVTESSLAFTHPSTQQQMLMWKSTPKTVLLLKKLGQE 720

Query: 721  LMEEAKEAASFLYHQEKMNVLVEPDVHDIFARIPGFGFVQTFYIQDTSDLHEKVDFVACL 780
            LMEEAKE ASFL+HQEKM+VLVEPDVHDIFARIPGFGFVQTFY QDTSDLHEKVD VACL
Sbjct: 721  LMEEAKEVASFLHHQEKMHVLVEPDVHDIFARIPGFGFVQTFYSQDTSDLHEKVDLVACL 780

Query: 781  GGDGVILHASNLFRGAVPPVVSFNLGSLGFLTSHTFESYRQDLRQVIHGNDSRDGVYITL 840
            GGDGVILHASNLFRGAVPPVVSFNLGSLGFLTSHTF+SYRQDLRQVIHGNDSRDGVYITL
Sbjct: 781  GGDGVILHASNLFRGAVPPVVSFNLGSLGFLTSHTFDSYRQDLRQVIHGNDSRDGVYITL 840

Query: 841  RMRLQCEIFRNGKAIPGKLFNILNEVVVDRGSNPYLSKIECYEHDRLITKVQGDGVIVAT 900
            RMRLQCEIFRNGKAIPGKLFNILNEVVVDRGSNPYLSKIECYEHDRLITKVQGDGVIVAT
Sbjct: 841  RMRLQCEIFRNGKAIPGKLFNILNEVVVDRGSNPYLSKIECYEHDRLITKVQGDGVIVAT 900

Query: 901  PTGSTAYSTAAGGSMVHPNVPCMLFTPICPHSLSFRPVILPDSARLELKIPEDTRSNAWV 960
            PTGSTAYSTAAGGSMVHPNVPCMLFTPICPHSLSFRPVILPDSARLELKIPED RSNAWV
Sbjct: 901  PTGSTAYSTAAGGSMVHPNVPCMLFTPICPHSLSFRPVILPDSARLELKIPEDARSNAWV 960

Query: 961  SFDGKRRQQLSRGDSVRISMSSHPLPTVNKSDQTGDWFRSLIRCLNWNERLDQKAL 1017
            SFDGKRRQQLSRGDSVRISMS HPLPTVNKSDQTGDWFRSLIRCLNWNERLDQKAL
Sbjct: 961  SFDGKRRQQLSRGDSVRISMSRHPLPTVNKSDQTGDWFRSLIRCLNWNERLDQKAL 1016

BLAST of Sgr014308 vs. ExPASy TrEMBL
Match: A0A6J1GVE5 (LOW QUALITY PROTEIN: NAD kinase 2, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111457831 PE=3 SV=1)

HSP 1 Score: 1741.9 bits (4510), Expect = 0.0e+00
Identity = 873/1020 (85.59%), Postives = 929/1020 (91.08%), Query Frame = 0

Query: 1    MVVCCFEWHLDAIAVGMNRSLPATVIHS--YLSPLRPLLPSC-NARFLGFQLQTWKRLRR 60
            M++CCF WHLDAIA+GMNRSLPATVIHS  YLSP RPLLPS  NARFLGFQL TWKR RR
Sbjct: 1    MLLCCFVWHLDAIALGMNRSLPATVIHSYPYLSPFRPLLPSSNNARFLGFQLHTWKRFRR 60

Query: 61   RLKFAVTAELSKSLSLNSGSEFQLPWIGPVPGDIAEVEAYCRIFRTAERLHSVLMDTLCN 120
            RLKFAVTAELSKS+S NS SEFQL W+GPVPGD+AEVEAYCRIFRTAERLHSVLMDTLCN
Sbjct: 61   RLKFAVTAELSKSVSFNSASEFQLSWMGPVPGDVAEVEAYCRIFRTAERLHSVLMDTLCN 120

Query: 121  PLTGECSVSYDVKPGKNPLIEDKIVSVLGCIVSLLNKGREDVLSGRSSAMNTFQVANLDA 180
            P TGECSVSYDV PG+NP+IEDKIVSVLGCIVSLLNKG+EDVLSGRSSAMN F+  NLDA
Sbjct: 121  PFTGECSVSYDVTPGENPVIEDKIVSVLGCIVSLLNKGKEDVLSGRSSAMNAFRGDNLDA 180

Query: 181  MEDDLPPLAAFRSEMKRCCESLHVALENFLTPGDDRSLDVWRKLQRLKNVCYDSGFSRGE 240
            MED+LPPLAAFRSEMKRCCESLHVALENFLTP DDRS DVWRKLQRLKNVCYDSGF RGE
Sbjct: 181  MEDNLPPLAAFRSEMKRCCESLHVALENFLTPDDDRSWDVWRKLQRLKNVCYDSGFPRGE 240

Query: 241  AYPCHTLFANWNPVYLHNFKEETLAKKSDVAFWSGGQVTEEGLKWLIERGFKTIVDLRAE 300
             YPCHTLFANWNPVYLHN KEE  AK SDV+FWSGGQ+TEEGLKWLIERGFKTIVDLRAE
Sbjct: 241  DYPCHTLFANWNPVYLHNSKEEVSAKNSDVSFWSGGQITEEGLKWLIERGFKTIVDLRAE 300

Query: 301  TVKDDFHTALLQDAIGSAKVKVIKIPVEVRTAPSVDQVEKFASLVSDGSNRLIYLHSKEG 360
            TVKD F++A L DAIGS KVKVI+IPVEVRTAP+ DQVEKFASLVSDGSN LIYLHSKEG
Sbjct: 301  TVKDIFYSASLHDAIGSDKVKVIRIPVEVRTAPTADQVEKFASLVSDGSNGLIYLHSKEG 360

Query: 361  VWRTSAMISRWRQYVTRSGSQFVSNQTI-GGDIPLRDTSPKLAHNQNGAKESLEIFTIQE 420
            +WRTSAMISRWRQY TRSGSQFVSNQTI   DIPL DTSPKL  N +GAKESLEI T+++
Sbjct: 361  LWRTSAMISRWRQYATRSGSQFVSNQTIVPADIPLGDTSPKLVQNPDGAKESLEISTVEK 420

Query: 421  TFPREEDNQSLLVESAHHSSIDVIGKTNYAETDKGSQNVNGAYNRPIPSQDLSSLRAVDS 480
            T   +E+++SLL+ESA H SI+   + N  E DK  QN NGA+N  IP+QDL+SLRA  +
Sbjct: 421  TSSCDENSRSLLLESADHCSIN---RKNNTEADKIIQNENGAFNGSIPTQDLTSLRADHN 480

Query: 481  GEEYLSQFSIETDPLKAQIPPCNIFSRKEMSNFFRTKKISPPNYFNHQMKRKEKWPLSIE 540
            GEE+LSQFS+ TDPLKAQIPPCNIFSRKEMSNFFRTKKISP NY + +MK  EK P S E
Sbjct: 481  GEEHLSQFSVATDPLKAQIPPCNIFSRKEMSNFFRTKKISPQNYLHRKMKTNEKRPPSTE 540

Query: 541  LPASRIQRFSVDDSNLKSGFVEAENFTGSASVKDSSSKTHYLSTTKMKYVSGDSHVSANP 600
            LP SR+Q+FSVD+S+LKSG VEA N  G  SVK++SS THYLSTT M+YV+GDSHVS+NP
Sbjct: 541  LPMSRVQKFSVDNSDLKSGLVEAGNTNGRPSVKETSSTTHYLSTTNMRYVNGDSHVSSNP 600

Query: 601  VLNGLGVDGRNPLTTEASTAVGGTFQSKSEKKDLKSNGQATSVSGNVDMESVEGNMCASA 660
            V N LGVDGRNPL TEASTAVG  FQSK+E   LKSNGQATSVS N +MESVEGNMCASA
Sbjct: 601  VHNELGVDGRNPLMTEASTAVGDKFQSKAETNSLKSNGQATSVSSNDEMESVEGNMCASA 660

Query: 661  TGVVRVQSRKKAEMFLVRTDGFSCAREKVTESSLAFTHPSTQQQMLMWKSTPKTVLLLKK 720
            TGVVRVQSR+KAEMFLVRTDGFSCAREKVTESSLAFTHPSTQQQMLMWKSTP+TVLLLKK
Sbjct: 661  TGVVRVQSRRKAEMFLVRTDGFSCAREKVTESSLAFTHPSTQQQMLMWKSTPRTVLLLKK 720

Query: 721  LGQELMEEAKEAASFLYHQEKMNVLVEPDVHDIFARIPGFGFVQTFYIQDTSDLHEKVDF 780
            LG+ELMEEAKE ASF+YHQEKMNVLVEPDVHDIFARIPGFGFVQTFY QDTSDLHEKVDF
Sbjct: 721  LGEELMEEAKEVASFMYHQEKMNVLVEPDVHDIFARIPGFGFVQTFYSQDTSDLHEKVDF 780

Query: 781  VACLGGDGVILHASNLFRGAVPPVVSFNLGSLGFLTSHTFESYRQDLRQVIHGNDSRDGV 840
            VACLGGDGVILHASN+FRGAVPPVVSFNLGSLGFLTSHTF+SYRQDLRQVIHGNDS DGV
Sbjct: 781  VACLGGDGVILHASNIFRGAVPPVVSFNLGSLGFLTSHTFDSYRQDLRQVIHGNDSLDGV 840

Query: 841  YITLRMRLQCEIFRNGKAIPGKLFNILNEVVVDRGSNPYLSKIECYEHDRLITKVQGDGV 900
            YITLRMRLQCEIFRNGKAIPGK+FNILNEVVVDRGSNPYLSKIECYEHDRLITKVQGDGV
Sbjct: 841  YITLRMRLQCEIFRNGKAIPGKVFNILNEVVVDRGSNPYLSKIECYEHDRLITKVQGDGV 900

Query: 901  IVATPTGSTAYSTAAGGSMVHPNVPCMLFTPICPHSLSFRPVILPDSARLELKIPEDTRS 960
            IVATPTGSTAYSTAAGGSMVHPNVPCMLFTPICPHSLSFRPVILPDSARLELKIPED RS
Sbjct: 901  IVATPTGSTAYSTAAGGSMVHPNVPCMLFTPICPHSLSFRPVILPDSARLELKIPEDARS 960

Query: 961  NAWVSFDGKRRQQLSRGDSVRISMSSHPLPTVNKSDQTGDWFRSLIRCLNWNERLDQKAL 1017
            NAWVSFDGKRRQQLSRGDSV+ISMS HPLPTVNKSDQTGDWFRSLIRCLNWNERLDQKAL
Sbjct: 961  NAWVSFDGKRRQQLSRGDSVKISMSRHPLPTVNKSDQTGDWFRSLIRCLNWNERLDQKAL 1017

BLAST of Sgr014308 vs. ExPASy TrEMBL
Match: A0A6J1JQZ5 (LOW QUALITY PROTEIN: NAD kinase 2, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111487544 PE=3 SV=1)

HSP 1 Score: 1741.5 bits (4509), Expect = 0.0e+00
Identity = 874/1020 (85.69%), Postives = 929/1020 (91.08%), Query Frame = 0

Query: 1    MVVCCFEWHLDAIAVGMNRSLPATVIHS--YLSPLRPLLPSC-NARFLGFQLQTWKRLRR 60
            M++CCF WHLDAIAVGMNRSLPATVIHS  YLSP R LLPS  NARFLGFQL TW+R RR
Sbjct: 1    MLLCCFVWHLDAIAVGMNRSLPATVIHSYPYLSPFRLLLPSSNNARFLGFQLHTWRRFRR 60

Query: 61   RLKFAVTAELSKSLSLNSGSEFQLPWIGPVPGDIAEVEAYCRIFRTAERLHSVLMDTLCN 120
            RLKFAVTAELSKS+S NS SEFQL W+GPVPGDIAEVEAYCRIFRTAERLHSVLMDTLCN
Sbjct: 61   RLKFAVTAELSKSVSFNSASEFQLSWMGPVPGDIAEVEAYCRIFRTAERLHSVLMDTLCN 120

Query: 121  PLTGECSVSYDVKPGKNPLIEDKIVSVLGCIVSLLNKGREDVLSGRSSAMNTFQVANLDA 180
            P TGECSVSYDV PG+NP+IEDKIVSVLGCIVSLLNKG+EDVLSGRSSAMN F+  NLDA
Sbjct: 121  PFTGECSVSYDVTPGENPVIEDKIVSVLGCIVSLLNKGKEDVLSGRSSAMNAFRGDNLDA 180

Query: 181  MEDDLPPLAAFRSEMKRCCESLHVALENFLTPGDDRSLDVWRKLQRLKNVCYDSGFSRGE 240
            MED+LPPLAAFRSEMKRCCESLHVALENFLTP DDRS DVWRKLQRLKNVCYDSGF RGE
Sbjct: 181  MEDNLPPLAAFRSEMKRCCESLHVALENFLTPDDDRSWDVWRKLQRLKNVCYDSGFPRGE 240

Query: 241  AYPCHTLFANWNPVYLHNFKEETLAKKSDVAFWSGGQVTEEGLKWLIERGFKTIVDLRAE 300
             YPCHTLFANWNPVYLHN KEE  AK SDV+FWSGGQ+TEEGLKWLIERGFKTIVDLRAE
Sbjct: 241  DYPCHTLFANWNPVYLHNSKEEVSAKNSDVSFWSGGQITEEGLKWLIERGFKTIVDLRAE 300

Query: 301  TVKDDFHTALLQDAIGSAKVKVIKIPVEVRTAPSVDQVEKFASLVSDGSNRLIYLHSKEG 360
            TVKD F++A L DAIGS KVKVI+IPVEVRTAP+ DQVEKFASLVSDGSN L+YLHSKEG
Sbjct: 301  TVKDIFYSASLHDAIGSDKVKVIRIPVEVRTAPTADQVEKFASLVSDGSNGLLYLHSKEG 360

Query: 361  VWRTSAMISRWRQYVTRSGSQFVSNQTI-GGDIPLRDTSPKLAHNQNGAKESLEIFTIQE 420
            VWRTSAMISRWRQY TRSGSQFVSNQTI   DIPL DTSPKL  N NGAKESLEI T+++
Sbjct: 361  VWRTSAMISRWRQYATRSGSQFVSNQTIVPADIPLGDTSPKLVQNPNGAKESLEISTVEK 420

Query: 421  TFPREEDNQSLLVESAHHSSIDVIGKTNYAETDKGSQNVNGAYNRPIPSQDLSSLRAVDS 480
            T   +E++QSLL+ESA H SI+   + N AE DK  QN NGA+N  IP+QDL+SLRA  +
Sbjct: 421  TSSCDENSQSLLLESADHRSIN---RKNNAEADKIFQNENGAFNGAIPTQDLTSLRADHN 480

Query: 481  GEEYLSQFSIETDPLKAQIPPCNIFSRKEMSNFFRTKKISPPNYFNHQMKRKEKWPLSIE 540
            GEE+LSQFS+ TDPLKAQIPPCNIFSRKEMSNFFRTKKISP NY + +MK  EK P S E
Sbjct: 481  GEEHLSQFSVATDPLKAQIPPCNIFSRKEMSNFFRTKKISPQNYLHRKMKTNEKRPPSTE 540

Query: 541  LPASRIQRFSVDDSNLKSGFVEAENFTGSASVKDSSSKTHYLSTTKMKYVSGDSHVSANP 600
            LP SR+Q+FSVD+S+LKSG VEA N  G  +VK++SS THYLSTT M+YV+GDSHVS+NP
Sbjct: 541  LPMSRVQKFSVDNSDLKSGLVEAGNTNGRPTVKETSSSTHYLSTTNMRYVNGDSHVSSNP 600

Query: 601  VLNGLGVDGRNPLTTEASTAVGGTFQSKSEKKDLKSNGQATSVSGNVDMESVEGNMCASA 660
            V N LGVDGRNPL TEASTAVG  FQSK+E   LKSNGQATSVS N +M+SVEGNMCASA
Sbjct: 601  VHNELGVDGRNPLMTEASTAVGDKFQSKAETNSLKSNGQATSVSSNDEMKSVEGNMCASA 660

Query: 661  TGVVRVQSRKKAEMFLVRTDGFSCAREKVTESSLAFTHPSTQQQMLMWKSTPKTVLLLKK 720
            TGVVRVQSR+KAEMFLVRTDGFSCAREKVTESSLAFTHPSTQQQMLMWKSTP+TVLLLKK
Sbjct: 661  TGVVRVQSRRKAEMFLVRTDGFSCAREKVTESSLAFTHPSTQQQMLMWKSTPRTVLLLKK 720

Query: 721  LGQELMEEAKEAASFLYHQEKMNVLVEPDVHDIFARIPGFGFVQTFYIQDTSDLHEKVDF 780
            LG+ELMEEAKE ASF+YHQEKMNVLVEPDVHDIFARIPGFGFVQTFY QDTSDLHEKVDF
Sbjct: 721  LGEELMEEAKEVASFMYHQEKMNVLVEPDVHDIFARIPGFGFVQTFYSQDTSDLHEKVDF 780

Query: 781  VACLGGDGVILHASNLFRGAVPPVVSFNLGSLGFLTSHTFESYRQDLRQVIHGNDSRDGV 840
            VACLGGDGVILHASN+FRGAVPPVVSFNLGSLGFLTSHTF+SYRQDLRQVIHGNDS DGV
Sbjct: 781  VACLGGDGVILHASNIFRGAVPPVVSFNLGSLGFLTSHTFDSYRQDLRQVIHGNDSLDGV 840

Query: 841  YITLRMRLQCEIFRNGKAIPGKLFNILNEVVVDRGSNPYLSKIECYEHDRLITKVQGDGV 900
            YITLRMRLQCEIFRNGKAIPGK+FNILNEVVVDRGSNPYLSKIECYEHDRLITKVQGDGV
Sbjct: 841  YITLRMRLQCEIFRNGKAIPGKVFNILNEVVVDRGSNPYLSKIECYEHDRLITKVQGDGV 900

Query: 901  IVATPTGSTAYSTAAGGSMVHPNVPCMLFTPICPHSLSFRPVILPDSARLELKIPEDTRS 960
            IVATPTGSTAYSTAAGGSMVHPNVPCMLFTPICPHSLSFRPVILPDSARLELKIPED RS
Sbjct: 901  IVATPTGSTAYSTAAGGSMVHPNVPCMLFTPICPHSLSFRPVILPDSARLELKIPEDARS 960

Query: 961  NAWVSFDGKRRQQLSRGDSVRISMSSHPLPTVNKSDQTGDWFRSLIRCLNWNERLDQKAL 1017
            NAWVSFDGKRRQQLSRGDSV+ISMS HPLPTVNKSDQTGDWFRSLIRCLNWNERLDQKAL
Sbjct: 961  NAWVSFDGKRRQQLSRGDSVKISMSRHPLPTVNKSDQTGDWFRSLIRCLNWNERLDQKAL 1017

BLAST of Sgr014308 vs. ExPASy TrEMBL
Match: A0A1S3CS14 (NAD kinase 2, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LOC103504095 PE=3 SV=1)

HSP 1 Score: 1695.6 bits (4390), Expect = 0.0e+00
Identity = 861/1019 (84.49%), Postives = 916/1019 (89.89%), Query Frame = 0

Query: 1    MVVCCFEWHLDAIAVGMNRSLPATVIHSYLSPLRPLLPSC-NARFLGFQLQTWKRLRRRL 60
            MV+CCF+WHLDAIAV MNRSLP ++IHSYLSP   L PS  NARFLGFQ  TW  +RRRL
Sbjct: 1    MVLCCFQWHLDAIAVAMNRSLPPSLIHSYLSPFPLLFPSSNNARFLGFQCHTWNPIRRRL 60

Query: 61   KFAVTAELSKSL-SLNSGSEFQLPWIGPVPGDIAEVEAYCRIFRTAERLHSVLMDTLCNP 120
             FAVTA++SKS  SL+S S+FQLPW+GPVPGDIAEVEAYCRIFRTAERLHSVLMDTLCNP
Sbjct: 61   NFAVTADISKSSPSLHSASDFQLPWVGPVPGDIAEVEAYCRIFRTAERLHSVLMDTLCNP 120

Query: 121  LTGECSVSYDVKPGKNPLIEDKIVSVLGCIVSLLNKGREDVLSGRSSAMNTFQVANLDAM 180
             TGECSVSYD+ PG+NPLIEDKIVSVLGC+VSL+NKGREDVLSGRSSAMN+F+ ANLDA 
Sbjct: 121  FTGECSVSYDISPGENPLIEDKIVSVLGCLVSLINKGREDVLSGRSSAMNSFRGANLDAT 180

Query: 181  EDDLPPLAAFRSEMKRCCESLHVALENFLTPGDDRSLDVWRKLQRLKNVCYDSGFSRGEA 240
            ED+LPPLAAFRSEMKRCCESLHVALENFL PGD+RSLDVWRKLQRLKNVCYDSGF+RGE 
Sbjct: 181  EDNLPPLAAFRSEMKRCCESLHVALENFLIPGDERSLDVWRKLQRLKNVCYDSGFTRGED 240

Query: 241  YPCHTLFANWNPVYLHNFKEETLAKKSDVAFWSGGQVTEEGLKWLIERGFKTIVDLRAET 300
            YPCHTLFANWNPVYLHNFK+ET AK SDVAFWSGGQVTEEGLKWLIERGFKTIVDLRAET
Sbjct: 241  YPCHTLFANWNPVYLHNFKDETSAKNSDVAFWSGGQVTEEGLKWLIERGFKTIVDLRAET 300

Query: 301  VKDDFHTALLQDAIGSAKVKVIKIPVEVRTAPSVDQVEKFASLVSDGSNRLIYLHSKEGV 360
            VKD+F++A L DAIGS KVKVIKIPVE RTAP++DQVEKFASLVSDGSN LIYLHSKEGV
Sbjct: 301  VKDEFYSASLHDAIGSEKVKVIKIPVEARTAPTMDQVEKFASLVSDGSNGLIYLHSKEGV 360

Query: 361  WRTSAMISRWRQYVTRSGSQFVSNQTIGGDIPLR-DTSPKLAHNQNGAKESLEIFTIQET 420
            WRTSAMISRWRQY TRSGSQ VSNQTI   +P+  D+S  LA NQNGAKESLEI    ET
Sbjct: 361  WRTSAMISRWRQYATRSGSQIVSNQTI---VPVDIDSSSNLALNQNGAKESLEISITGET 420

Query: 421  FPREEDNQSLLVESAHHSSIDVIGKTNYAETDKGSQNVNGAYNRPIPSQDLSSLRAVDSG 480
            FP  ED+QSL++ESAHHS   +I + N AETD+ SQNVNGAYN P P+QD++SLRAV +G
Sbjct: 421  FPCAEDSQSLVLESAHHS---LINRKNNAETDEVSQNVNGAYNGPSPTQDMTSLRAVVNG 480

Query: 481  EEYLSQFSIETDPLKAQIPPCNIFSRKEMSNFFRTKKISPPNYFNHQMKRKEKWPLSIEL 540
                    IE DPLKAQIPPCNIFSRKEMSNFF+TKKISP +Y + +MK KEK+  S E+
Sbjct: 481  -------GIEIDPLKAQIPPCNIFSRKEMSNFFKTKKISPQSYLHRRMKTKEKF--STEV 540

Query: 541  PASRIQRFSVDDSNLKSGFVEAENFTGSASVKDSSSKTHYLSTTKMKYVSGDSHVSANPV 600
            PASR+ R SV++S+ KSG VEA NF GS SVKDSSSKT Y+S  KM Y +GDSHVSANPV
Sbjct: 541  PASRVLRSSVNNSD-KSGIVEAGNFNGSPSVKDSSSKTQYVSAMKMNYGNGDSHVSANPV 600

Query: 601  LNGLGVDGRNPLTTEASTAVGGTFQSKSEKKDLKSNGQATSVSGNVDMESVEGNMCASAT 660
              GL VDGRNPLTT AS AVGG   SKSE  DLKSNGQATSVS N ++ESVEGNMCASAT
Sbjct: 601  FVGLEVDGRNPLTTVASAAVGGKVPSKSEINDLKSNGQATSVSSNGNVESVEGNMCASAT 660

Query: 661  GVVRVQSRKKAEMFLVRTDGFSCAREKVTESSLAFTHPSTQQQMLMWKSTPKTVLLLKKL 720
            GVVRVQSR+KAEMFLVRTDGFSCAREKVTESSLAFTHPSTQQQMLMWKSTPKTVLLLKKL
Sbjct: 661  GVVRVQSRRKAEMFLVRTDGFSCAREKVTESSLAFTHPSTQQQMLMWKSTPKTVLLLKKL 720

Query: 721  GQELMEEAKEAASFLYHQEKMNVLVEPDVHDIFARIPGFGFVQTFYIQDTSDLHEKVDFV 780
            GQELMEEAKE A FLYHQEKM VLVEPD+HDIFARIPGFGFVQTFY QDTSDLHEKVDFV
Sbjct: 721  GQELMEEAKEVALFLYHQEKMTVLVEPDIHDIFARIPGFGFVQTFYSQDTSDLHEKVDFV 780

Query: 781  ACLGGDGVILHASNLFRGAVPPVVSFNLGSLGFLTSHTFESYRQDLRQVIHGNDSRDGVY 840
            ACLGGDGVILHASNLFR AVPPVVSFNLGSLGFLTSHTF+SYRQDLRQVIHGNDS DGVY
Sbjct: 781  ACLGGDGVILHASNLFRSAVPPVVSFNLGSLGFLTSHTFDSYRQDLRQVIHGNDSLDGVY 840

Query: 841  ITLRMRLQCEIFRNGKAIPGKLFNILNEVVVDRGSNPYLSKIECYEHDRLITKVQGDGVI 900
            ITLRMRLQCEIFRNGKAIPGKLFNILNEVVVDRGSNPYLSKIECYEHDRLITKVQGDGVI
Sbjct: 841  ITLRMRLQCEIFRNGKAIPGKLFNILNEVVVDRGSNPYLSKIECYEHDRLITKVQGDGVI 900

Query: 901  VATPTGSTAYSTAAGGSMVHPNVPCMLFTPICPHSLSFRPVILPDSARLELKIPEDTRSN 960
            VATPTGSTAYSTAAGGSMVHPNVPCMLFTPICPHSLSFRPVILPDSARLELKIPED RSN
Sbjct: 901  VATPTGSTAYSTAAGGSMVHPNVPCMLFTPICPHSLSFRPVILPDSARLELKIPEDARSN 960

Query: 961  AWVSFDGKRRQQLSRGDSVRISMSSHPLPTVNKSDQTGDWFRSLIRCLNWNERLDQKAL 1017
            AWVSFDGKRRQQLSRGDSVRISMS HPLPTVNKSDQTGDWFRSLIRCLNWNERLDQKAL
Sbjct: 961  AWVSFDGKRRQQLSRGDSVRISMSRHPLPTVNKSDQTGDWFRSLIRCLNWNERLDQKAL 1003

BLAST of Sgr014308 vs. ExPASy TrEMBL
Match: A0A5A7UI58 (NAD kinase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold221G00930 PE=3 SV=1)

HSP 1 Score: 1682.5 bits (4356), Expect = 0.0e+00
Identity = 856/1012 (84.58%), Postives = 909/1012 (89.82%), Query Frame = 0

Query: 8    WHLDAIAVGMNRSLPATVIHSYLSPLRPLLPSC-NARFLGFQLQTWKRLRRRLKFAVTAE 67
            WHLDAIAV MNRSLP ++IHSYLSP   L PS  NARFLGFQ  TW  +RRRL FAVTA+
Sbjct: 4    WHLDAIAVAMNRSLPPSLIHSYLSPFPLLFPSSNNARFLGFQCHTWNPIRRRLNFAVTAD 63

Query: 68   LSKSL-SLNSGSEFQLPWIGPVPGDIAEVEAYCRIFRTAERLHSVLMDTLCNPLTGECSV 127
            +SKS  SL+S S+FQLPW+GPVPGDIAEVEAYCRIFRTAERLHSVLMDTLCNP TGECSV
Sbjct: 64   ISKSSPSLHSASDFQLPWVGPVPGDIAEVEAYCRIFRTAERLHSVLMDTLCNPFTGECSV 123

Query: 128  SYDVKPGKNPLIEDKIVSVLGCIVSLLNKGREDVLSGRSSAMNTFQVANLDAMEDDLPPL 187
            SYD+ PG+NPLIEDKIVSVLGC+VSL+NKGREDVLSGRSSAMN+F+ ANLDA ED+LPPL
Sbjct: 124  SYDISPGENPLIEDKIVSVLGCLVSLINKGREDVLSGRSSAMNSFRGANLDATEDNLPPL 183

Query: 188  AAFRSEMKRCCESLHVALENFLTPGDDRSLDVWRKLQRLKNVCYDSGFSRGEAYPCHTLF 247
            AAFRSEMKRCCESLHVALENFL PGD+RSLDVWRKLQRLKNVCYDSGF+RGE YPCHTLF
Sbjct: 184  AAFRSEMKRCCESLHVALENFLIPGDERSLDVWRKLQRLKNVCYDSGFTRGEDYPCHTLF 243

Query: 248  ANWNPVYLHNFKEETLAKKSDVAFWSGGQVTEEGLKWLIERGFKTIVDLRAETVKDDFHT 307
            ANWNPVYLHNFK+ET AK SDVAFWSGGQVTEEGLKWLIERGFKTIVDLRAETVKD+F++
Sbjct: 244  ANWNPVYLHNFKDETSAKNSDVAFWSGGQVTEEGLKWLIERGFKTIVDLRAETVKDEFYS 303

Query: 308  ALLQDAIGSAKVKVIKIPVEVRTAPSVDQVEKFASLVSDGSNRLIYLHSKEGVWRTSAMI 367
            A L DAIGS KVKVIKIPVE RTAP++DQVEKFASLVSDGSN LIYLHSKEGVWRTSAMI
Sbjct: 304  ASLHDAIGSEKVKVIKIPVEARTAPTMDQVEKFASLVSDGSNGLIYLHSKEGVWRTSAMI 363

Query: 368  SRWRQYVTRSGSQFVSNQTIGGDIPLR-DTSPKLAHNQNGAKESLEIFTIQETFPREEDN 427
            SRWRQY TRSGSQ VSNQTI   +P+  D+S  LA NQNGAKESLEI    ETFP  ED+
Sbjct: 364  SRWRQYATRSGSQIVSNQTI---VPVDIDSSSNLALNQNGAKESLEISITGETFPCAEDS 423

Query: 428  QSLLVESAHHSSIDVIGKTNYAETDKGSQNVNGAYNRPIPSQDLSSLRAVDSGEEYLSQF 487
            QSL++ESAHHS   +I + N AETD+ SQNVNGAYN P P+QD++SLRAV +G       
Sbjct: 424  QSLVLESAHHS---LINRKNNAETDEVSQNVNGAYNGPSPTQDMTSLRAVVNG------- 483

Query: 488  SIETDPLKAQIPPCNIFSRKEMSNFFRTKKISPPNYFNHQMKRKEKWPLSIELPASRIQR 547
             IE DPLKAQIPPCNIFSRKEMSNFF+TKKISP +Y + +MK KEK+  S E+PASR+ R
Sbjct: 484  GIEIDPLKAQIPPCNIFSRKEMSNFFKTKKISPQSYLHRRMKTKEKF--STEVPASRVLR 543

Query: 548  FSVDDSNLKSGFVEAENFTGSASVKDSSSKTHYLSTTKMKYVSGDSHVSANPVLNGLGVD 607
             SV++S+ KSG VEA NF GS SVKDSSSKT Y+S  KM Y +GDSHVSANPV  GL VD
Sbjct: 544  SSVNNSD-KSGIVEAGNFNGSPSVKDSSSKTQYVSAMKMNYGNGDSHVSANPVFVGLEVD 603

Query: 608  GRNPLTTEASTAVGGTFQSKSEKKDLKSNGQATSVSGNVDMESVEGNMCASATGVVRVQS 667
            GRNPLTT AS AVGG   SKSE  DLKSNGQATSVS N ++ESVEGNMCASATGVVRVQS
Sbjct: 604  GRNPLTTVASAAVGGKVPSKSEINDLKSNGQATSVSSNGNVESVEGNMCASATGVVRVQS 663

Query: 668  RKKAEMFLVRTDGFSCAREKVTESSLAFTHPSTQQQMLMWKSTPKTVLLLKKLGQELMEE 727
            R+KAEMFLVRTDGFSCAREKVTESSLAFTHPSTQQQMLMWKSTPKTVLLLKKLGQELMEE
Sbjct: 664  RRKAEMFLVRTDGFSCAREKVTESSLAFTHPSTQQQMLMWKSTPKTVLLLKKLGQELMEE 723

Query: 728  AKEAASFLYHQEKMNVLVEPDVHDIFARIPGFGFVQTFYIQDTSDLHEKVDFVACLGGDG 787
            AKE A FLYHQEKM VLVEPD+HDIFARIPGFGFVQTFY QDTSDLHEKVDFVACLGGDG
Sbjct: 724  AKEVALFLYHQEKMTVLVEPDIHDIFARIPGFGFVQTFYSQDTSDLHEKVDFVACLGGDG 783

Query: 788  VILHASNLFRGAVPPVVSFNLGSLGFLTSHTFESYRQDLRQVIHGNDSRDGVYITLRMRL 847
            VILHASNLFR AVPPVVSFNLGSLGFLTSHTF+SYRQDLRQVIHGNDS DGVYITLRMRL
Sbjct: 784  VILHASNLFRSAVPPVVSFNLGSLGFLTSHTFDSYRQDLRQVIHGNDSLDGVYITLRMRL 843

Query: 848  QCEIFRNGKAIPGKLFNILNEVVVDRGSNPYLSKIECYEHDRLITKVQGDGVIVATPTGS 907
            QCEIFRNGKAIPGKLFNILNEVVVDRGSNPYLSKIECYEHDRLITKVQGDGVIVATPTGS
Sbjct: 844  QCEIFRNGKAIPGKLFNILNEVVVDRGSNPYLSKIECYEHDRLITKVQGDGVIVATPTGS 903

Query: 908  TAYSTAAGGSMVHPNVPCMLFTPICPHSLSFRPVILPDSARLELKIPEDTRSNAWVSFDG 967
            TAYSTAAGGSMVHPNVPCMLFTPICPHSLSFRPVILPDSARLELKIPED RSNAWVSFDG
Sbjct: 904  TAYSTAAGGSMVHPNVPCMLFTPICPHSLSFRPVILPDSARLELKIPEDARSNAWVSFDG 963

Query: 968  KRRQQLSRGDSVRISMSSHPLPTVNKSDQTGDWFRSLIRCLNWNERLDQKAL 1017
            KRRQQLSRGDSVRISMS HPLPTVNKSDQTGDWFRSLIRCLNWNERLDQKAL
Sbjct: 964  KRRQQLSRGDSVRISMSRHPLPTVNKSDQTGDWFRSLIRCLNWNERLDQKAL 999

BLAST of Sgr014308 vs. TAIR 10
Match: AT1G21640.1 (NAD kinase 2 )

HSP 1 Score: 1197.6 bits (3097), Expect = 0.0e+00
Identity = 636/1022 (62.23%), Postives = 748/1022 (73.19%), Query Frame = 0

Query: 17   MNRSLPATVIHSYLSPLRPLLPSCNARFLGFQLQTWKR---LRRRLKFAVTAELSKSLSL 76
            M+R  PAT I S L     L  S + R + F  +  +     +RRL+F + A+LS++ S 
Sbjct: 13   MSRLSPATGISSRLRFSIGL--SSDGRLIPFGFRFRRNDVPFKRRLRFVIRAQLSEAFSP 72

Query: 77   NSGSEFQ---------LPWIGPVPGDIAEVEAYCRIFRTAERLHSVLMDTLCNPLTGECS 136
            + G + Q         LPWIGPVPGDIAEVEAYCRIFR+AERLH  LM+TLCNP+TGEC 
Sbjct: 73   DLGLDSQAVKSRDTSNLPWIGPVPGDIAEVEAYCRIFRSAERLHGALMETLCNPVTGECR 132

Query: 137  VSYDVKPGKNPLIEDKIVSVLGCIVSLLNKGREDVLSGRSSAMNTFQVANLDAMEDDLPP 196
            V YD  P + PL+EDKIVSVLGCI+SLLNKGR+++LSGRSS+MN+F + ++   E+ LPP
Sbjct: 133  VPYDFSPEEKPLLEDKIVSVLGCILSLLNKGRKEILSGRSSSMNSFNLDDVGVAEESLPP 192

Query: 197  LAAFRSEMKRCCESLHVALENFLTPGDDRSLDVWRKLQRLKNVCYDSGFSRGEAYPCHTL 256
            LA FR EMKRCCESLH+ALEN+LTP D+RS  VWRKLQ+LKNVCYD+GF R + YPC TL
Sbjct: 193  LAVFRGEMKRCCESLHIALENYLTPDDERSGIVWRKLQKLKNVCYDAGFPRSDNYPCQTL 252

Query: 257  FANWNPVYLHNFKEETLAKKSDVAFWSGGQVTEEGLKWLIERGFKTIVDLRAETVKDDFH 316
            FANW+P+Y  N KE+  + +S++AFW GGQVT+EGLKWLIE GFKTIVDLRAE VKD F+
Sbjct: 253  FANWDPIYSSNTKEDIDSYESEIAFWRGGQVTQEGLKWLIENGFKTIVDLRAEIVKDTFY 312

Query: 317  TALLQDAIGSAKVKVIKIPVEVRTAPSVDQVEKFASLVSDGSNRLIYLHSKEGVWRTSAM 376
               L DAI   K+ V++IP++VR AP  +QVE FAS+VSD S R IY+HSKEGVWRTSAM
Sbjct: 313  QTALDDAISLGKITVVQIPIDVRMAPKAEQVELFASIVSDSSKRPIYVHSKEGVWRTSAM 372

Query: 377  ISRWRQYVTRSGSQFVSNQTIGGDIPLRDTSPKLAHNQNGAKESLEIFTIQETFPREEDN 436
            +SRW+QY+TR          I  +IP                       + E   R E +
Sbjct: 373  VSRWKQYMTR---------PITKEIP-----------------------VSEESKRREVS 432

Query: 437  QSLLVESAHHSSIDVIGKTNYAETDKGSQNVNGAYNRPIPSQDLSSLRAVDSGEEYLSQF 496
            ++ L  +A  S   V  +    +TDK S+ +N   +R   SQ   S R    G+   S+F
Sbjct: 433  ETKLGSNAVVSGKGVPDE----QTDKVSE-INEVDSRSASSQSKESGRF--EGDTSASEF 492

Query: 497  SIETDPLKAQIPPCNIFSRKEMSNFFRTKKISPPNYFNHQMKRKEKWPLSIELPASRIQR 556
            ++ +DPLK+Q+PP NIFSRKEMS F ++K I+P  Y  +  K     P            
Sbjct: 493  NMVSDPLKSQVPPGNIFSRKEMSKFLKSKSIAPAGYLTNPSKILGTVPTPQFSYTGVTNG 552

Query: 557  FSVDDSNLKSGFVEAENFTGSASVKDSSSKTHYLSTTKMKYVSGDSHVSANPVLNGLGVD 616
              + D +      E  N  G+     S S    L     K+ +G+ H S N   N    D
Sbjct: 553  NQIVDKDSIRRLAETGNSNGTLLPTSSQS----LDFGNGKFSNGNVHASDN--TNKSISD 612

Query: 617  GRN----------PLTTEASTAVGGTFQSKSEKKDLKSNGQATSVSGNVDMESVEGNMCA 676
             R           P +   S AVG    S  E +  ++N  ++S S + +  ++EGNMCA
Sbjct: 613  NRGNGFSAAPIAVPPSDNLSRAVGS--HSVRESQTQRNNSGSSSDSSDDEAGAIEGNMCA 672

Query: 677  SATGVVRVQSRKKAEMFLVRTDGFSCAREKVTESSLAFTHPSTQQQMLMWKSTPKTVLLL 736
            SATGVVRVQSRKKAEMFLVRTDG SC REKVTESSLAFTHPSTQQQML+WK+TPKTVLLL
Sbjct: 673  SATGVVRVQSRKKAEMFLVRTDGVSCTREKVTESSLAFTHPSTQQQMLLWKTTPKTVLLL 732

Query: 737  KKLGQELMEEAKEAASFLYHQEKMNVLVEPDVHDIFARIPGFGFVQTFYIQDTSDLHEKV 796
            KKLGQELMEEAKEAASFLYHQE MNVLVEP+VHD+FARIPGFGFVQTFYIQDTSDLHE+V
Sbjct: 733  KKLGQELMEEAKEAASFLYHQENMNVLVEPEVHDVFARIPGFGFVQTFYIQDTSDLHERV 792

Query: 797  DFVACLGGDGVILHASNLFRGAVPPVVSFNLGSLGFLTSHTFESYRQDLRQVIHGNDSRD 856
            DFVACLGGDGVILHASNLF+GAVPPVVSFNLGSLGFLTSH FE +RQDL++VIHGN++ D
Sbjct: 793  DFVACLGGDGVILHASNLFKGAVPPVVSFNLGSLGFLTSHPFEDFRQDLKRVIHGNNTLD 852

Query: 857  GVYITLRMRLQCEIFRNGKAIPGKLFNILNEVVVDRGSNPYLSKIECYEHDRLITKVQGD 916
            GVYITLRMRL+CEI+R GKA+PGK+F++LNE+VVDRGSNPYLSKIECYEHDRLITKVQGD
Sbjct: 853  GVYITLRMRLRCEIYRKGKAMPGKVFDVLNEIVVDRGSNPYLSKIECYEHDRLITKVQGD 912

Query: 917  GVIVATPTGSTAYSTAAGGSMVHPNVPCMLFTPICPHSLSFRPVILPDSARLELKIPEDT 976
            GVIVATPTGSTAYSTAAGGSMVHPNVPCMLFTPICPHSLSFRPVILPDSA+LELKIP+D 
Sbjct: 913  GVIVATPTGSTAYSTAAGGSMVHPNVPCMLFTPICPHSLSFRPVILPDSAKLELKIPDDA 972

Query: 977  RSNAWVSFDGKRRQQLSRGDSVRISMSSHPLPTVNKSDQTGDWFRSLIRCLNWNERLDQK 1017
            RSNAWVSFDGKRRQQLSRGDSVRI MS HPLPTVNKSDQTGDWFRSLIRCLNWNERLDQK
Sbjct: 973  RSNAWVSFDGKRRQQLSRGDSVRIYMSQHPLPTVNKSDQTGDWFRSLIRCLNWNERLDQK 985

BLAST of Sgr014308 vs. TAIR 10
Match: AT1G21640.2 (NAD kinase 2 )

HSP 1 Score: 1187.9 bits (3072), Expect = 0.0e+00
Identity = 636/1036 (61.39%), Postives = 748/1036 (72.20%), Query Frame = 0

Query: 17   MNRSLPATVIHSYLSPLRPLLPSCNARFLGFQLQTWKR---LRRRLKFAVTAELSKSLSL 76
            M+R  PAT I S L     L  S + R + F  +  +     +RRL+F + A+LS++ S 
Sbjct: 13   MSRLSPATGISSRLRFSIGL--SSDGRLIPFGFRFRRNDVPFKRRLRFVIRAQLSEAFSP 72

Query: 77   NSGSEFQ---------LPWIGPVPGDIAEVEAYCRIFRTAERLHSVLMDTLCNPLTGECS 136
            + G + Q         LPWIGPVPGDIAEVEAYCRIFR+AERLH  LM+TLCNP+TGEC 
Sbjct: 73   DLGLDSQAVKSRDTSNLPWIGPVPGDIAEVEAYCRIFRSAERLHGALMETLCNPVTGECR 132

Query: 137  VSYDVKPGKNPLIEDKIVSVLGCIVSLLNKGREDVLSGRSSAMNTFQVANLDAMEDDLPP 196
            V YD  P + PL+EDKIVSVLGCI+SLLNKGR+++LSGRSS+MN+F + ++   E+ LPP
Sbjct: 133  VPYDFSPEEKPLLEDKIVSVLGCILSLLNKGRKEILSGRSSSMNSFNLDDVGVAEESLPP 192

Query: 197  LAAFRSEMKRCCESLHVALENFLTPGDDRSLDVWRKLQRLKNVCYDSGFSRGEAYPCHTL 256
            LA FR EMKRCCESLH+ALEN+LTP D+RS  VWRKLQ+LKNVCYD+GF R + YPC TL
Sbjct: 193  LAVFRGEMKRCCESLHIALENYLTPDDERSGIVWRKLQKLKNVCYDAGFPRSDNYPCQTL 252

Query: 257  FANWNPVYLHNFKEETLAKKSDVAFWSGGQVTEEGLKWLIERGFKTIVDLRAETVKDDFH 316
            FANW+P+Y  N KE+  + +S++AFW GGQVT+EGLKWLIE GFKTIVDLRAE VKD F+
Sbjct: 253  FANWDPIYSSNTKEDIDSYESEIAFWRGGQVTQEGLKWLIENGFKTIVDLRAEIVKDTFY 312

Query: 317  TALLQDAIGSAKVKVIKIPVEVRTAPSVDQVEKFASLVSDGSNRLIYLHSKEGVWRTSAM 376
               L DAI   K+ V++IP++VR AP  +QVE FAS+VSD S R IY+HSKEGVWRTSAM
Sbjct: 313  QTALDDAISLGKITVVQIPIDVRMAPKAEQVELFASIVSDSSKRPIYVHSKEGVWRTSAM 372

Query: 377  ISRWRQYVTRSGSQFVSNQTIGGDIPLRDTSPKLAHNQNGAKESLEIFTIQETFPREEDN 436
            +SRW+QY+TR          I  +IP                       + E   R E +
Sbjct: 373  VSRWKQYMTR---------PITKEIP-----------------------VSEESKRREVS 432

Query: 437  QSLLVESAHHSSIDVIGKTNYAETDKGSQNVNGAYNRPIPSQDLSSLRAVDSGEEYLSQF 496
            ++ L  +A  S   V  +    +TDK S+ +N   +R   SQ   S R    G+   S+F
Sbjct: 433  ETKLGSNAVVSGKGVPDE----QTDKVSE-INEVDSRSASSQSKESGRF--EGDTSASEF 492

Query: 497  SIETDPLKAQIPPCNIFSRKEMSNFFRTKKISPPNYFNHQMKRKEKWPLSIELPASRIQR 556
            ++ +DPLK+Q+PP NIFSRKEMS F ++K I+P  Y  +  K     P            
Sbjct: 493  NMVSDPLKSQVPPGNIFSRKEMSKFLKSKSIAPAGYLTNPSKILGTVPTPQFSYTGVTNG 552

Query: 557  FSVDDSNLKSGFVEAENFTGSASVKDSSSKTHYLSTTKMKYVSGDSHVSANPVLNGLGVD 616
              + D +      E  N  G+     S S    L     K+ +G+ H S N   N    D
Sbjct: 553  NQIVDKDSIRRLAETGNSNGTLLPTSSQS----LDFGNGKFSNGNVHASDN--TNKSISD 612

Query: 617  GRN----------PLTTEASTAVGGTFQSKSEKKDLKSNGQATSVSGNVDMESVEGNMCA 676
             R           P +   S AVG    S  E +  ++N  ++S S + +  ++EGNMCA
Sbjct: 613  NRGNGFSAAPIAVPPSDNLSRAVGS--HSVRESQTQRNNSGSSSDSSDDEAGAIEGNMCA 672

Query: 677  SATGVVRVQSRKKAEMFLVRTDGFSCAREKVTESSLAFTHPSTQQQMLMWKSTPKTVLLL 736
            SATGVVRVQSRKKAEMFLVRTDG SC REKVTESSLAFTHPSTQQQML+WK+TPKTVLLL
Sbjct: 673  SATGVVRVQSRKKAEMFLVRTDGVSCTREKVTESSLAFTHPSTQQQMLLWKTTPKTVLLL 732

Query: 737  KKLGQELMEEAKE--------------AASFLYHQEKMNVLVEPDVHDIFARIPGFGFVQ 796
            KKLGQELMEEAKE              AASFLYHQE MNVLVEP+VHD+FARIPGFGFVQ
Sbjct: 733  KKLGQELMEEAKEEVHEKLLVFQICFQAASFLYHQENMNVLVEPEVHDVFARIPGFGFVQ 792

Query: 797  TFYIQDTSDLHEKVDFVACLGGDGVILHASNLFRGAVPPVVSFNLGSLGFLTSHTFESYR 856
            TFYIQDTSDLHE+VDFVACLGGDGVILHASNLF+GAVPPVVSFNLGSLGFLTSH FE +R
Sbjct: 793  TFYIQDTSDLHERVDFVACLGGDGVILHASNLFKGAVPPVVSFNLGSLGFLTSHPFEDFR 852

Query: 857  QDLRQVIHGNDSRDGVYITLRMRLQCEIFRNGKAIPGKLFNILNEVVVDRGSNPYLSKIE 916
            QDL++VIHGN++ DGVYITLRMRL+CEI+R GKA+PGK+F++LNE+VVDRGSNPYLSKIE
Sbjct: 853  QDLKRVIHGNNTLDGVYITLRMRLRCEIYRKGKAMPGKVFDVLNEIVVDRGSNPYLSKIE 912

Query: 917  CYEHDRLITKVQGDGVIVATPTGSTAYSTAAGGSMVHPNVPCMLFTPICPHSLSFRPVIL 976
            CYEHDRLITKVQGDGVIVATPTGSTAYSTAAGGSMVHPNVPCMLFTPICPHSLSFRPVIL
Sbjct: 913  CYEHDRLITKVQGDGVIVATPTGSTAYSTAAGGSMVHPNVPCMLFTPICPHSLSFRPVIL 972

Query: 977  PDSARLELKIPEDTRSNAWVSFDGKRRQQLSRGDSVRISMSSHPLPTVNKSDQTGDWFRS 1017
            PDSA+LELKIP+D RSNAWVSFDGKRRQQLSRGDSVRI MS HPLPTVNKSDQTGDWFRS
Sbjct: 973  PDSAKLELKIPDDARSNAWVSFDGKRRQQLSRGDSVRIYMSQHPLPTVNKSDQTGDWFRS 999

BLAST of Sgr014308 vs. TAIR 10
Match: AT3G21070.2 (NAD kinase 1 )

HSP 1 Score: 277.3 bits (708), Expect = 4.8e-74
Identity = 150/327 (45.87%), Postives = 215/327 (65.75%), Query Frame = 0

Query: 696  STQQQMLMWKSTPKTVLLLKKLGQELMEE-AKEAASFLYHQEKMNVLVEPDV-HDIFARI 755
            S++Q  L W+S P+TVL++ K     +   + +   +L  Q+ +N+ VEP V  ++ +  
Sbjct: 201  SSKQISLTWESDPQTVLIITKPNSTSVRVLSVDMVRWLRTQKGLNIYVEPRVKEELLSES 260

Query: 756  PGFGFVQTFY-IQDTSDLHEKVDFVACLGGDGVILHASNLFRGAVPPVVSFNLGSLGFLT 815
              F FVQT+   ++ S LH KVD +  LGGDG +L A+++F+G VPP+V F++GSLGF+T
Sbjct: 261  SSFNFVQTWEDDKEISLLHTKVDLLITLGGDGTVLWAASMFKGPVPPIVPFSMGSLGFMT 320

Query: 816  SHTFESYRQDLRQVIHGNDSRDGVYITLRMRLQCEIFRNGKAI----PGKLFNILNEVVV 875
                E YR  L  ++ G      + ITLR RLQC I R+ KA     P +   +LNEV +
Sbjct: 321  PFHSEQYRDCLEAILKG-----PISITLRHRLQCHIIRD-KATHEYEPEETMLVLNEVTI 380

Query: 876  DRGSNPYLSKIECYEHDRLITKVQGDGVIVATPTGSTAYSTAAGGSMVHPNVPCMLFTPI 935
            DRG + YL+ +ECY  +  +T VQGDG+I++T +GSTAYS AAGGSMVHP VP +LFTPI
Sbjct: 381  DRGISSYLTNLECYCDNSFVTCVQGDGLILSTTSGSTAYSLAAGGSMVHPQVPGILFTPI 440

Query: 936  CPHSLSFRPVILPDSARLELKIPEDTRSNAWVSFDGKRRQQLSRGDSVRISMSSHPLPTV 995
            CPHSLSFRP+ILP+   + +++P ++RS+AWVSFDGK R+QL  GD++  SM+  P+ T 
Sbjct: 441  CPHSLSFRPLILPEHVTVRVQVPFNSRSSAWVSFDGKDRKQLEAGDALVCSMAPWPVSTA 500

Query: 996  NKSDQTGDWFRSLIRCLNWNERLDQKA 1016
             + + T D+ RS+   L+WN R  Q A
Sbjct: 501  CQVESTNDFLRSIHDGLHWNLRKTQSA 521

BLAST of Sgr014308 vs. TAIR 10
Match: AT3G21070.1 (NAD kinase 1 )

HSP 1 Score: 275.0 bits (702), Expect = 2.4e-73
Identity = 150/333 (45.05%), Postives = 215/333 (64.56%), Query Frame = 0

Query: 696  STQQQMLMWKSTPKTVLLLKKLGQELMEE-AKEAASFLYHQEKMNVLVEPDV-HDIFARI 755
            S++Q  L W+S P+TVL++ K     +   + +   +L  Q+ +N+ VEP V  ++ +  
Sbjct: 201  SSKQISLTWESDPQTVLIITKPNSTSVRVLSVDMVRWLRTQKGLNIYVEPRVKEELLSES 260

Query: 756  PGFGFVQTF-------YIQDTSDLHEKVDFVACLGGDGVILHASNLFRGAVPPVVSFNLG 815
              F FVQT+         ++ S LH KVD +  LGGDG +L A+++F+G VPP+V F++G
Sbjct: 261  SSFNFVQTWEDVMIYDADKEISLLHTKVDLLITLGGDGTVLWAASMFKGPVPPIVPFSMG 320

Query: 816  SLGFLTSHTFESYRQDLRQVIHGNDSRDGVYITLRMRLQCEIFRNGKAI----PGKLFNI 875
            SLGF+T    E YR  L  ++ G      + ITLR RLQC I R+ KA     P +   +
Sbjct: 321  SLGFMTPFHSEQYRDCLEAILKG-----PISITLRHRLQCHIIRD-KATHEYEPEETMLV 380

Query: 876  LNEVVVDRGSNPYLSKIECYEHDRLITKVQGDGVIVATPTGSTAYSTAAGGSMVHPNVPC 935
            LNEV +DRG + YL+ +ECY  +  +T VQGDG+I++T +GSTAYS AAGGSMVHP VP 
Sbjct: 381  LNEVTIDRGISSYLTNLECYCDNSFVTCVQGDGLILSTTSGSTAYSLAAGGSMVHPQVPG 440

Query: 936  MLFTPICPHSLSFRPVILPDSARLELKIPEDTRSNAWVSFDGKRRQQLSRGDSVRISMSS 995
            +LFTPICPHSLSFRP+ILP+   + +++P ++RS+AWVSFDGK R+QL  GD++  SM+ 
Sbjct: 441  ILFTPICPHSLSFRPLILPEHVTVRVQVPFNSRSSAWVSFDGKDRKQLEAGDALVCSMAP 500

Query: 996  HPLPTVNKSDQTGDWFRSLIRCLNWNERLDQKA 1016
             P+ T  + + T D+ RS+   L+WN R  Q A
Sbjct: 501  WPVSTACQVESTNDFLRSIHDGLHWNLRKTQSA 527