Sgr012991 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr012991 vs. NCBI nr
Match: XP_022155020.1 (probable carboxylesterase 17 [Momordica charantia])

HSP 1 Score: 584.3 bits (1505), Expect = 7.7e-163
Identity = 280/336 (83.33%), Postives = 300/336 (89.29%), Query Frame = 0

Query: 1   MATISFDPRFKLQMPIPHLQRGAIAEEIEGLIRVYADGHVERSPIIPNVSCSTIASDKGV 60
           MA IS DPR KLQMP  HLQ  + AEEIEGLIRVYADGHVER PIIPNV CST AS+KGV
Sbjct: 6   MAAISIDPRLKLQMPKSHLQCASTAEEIEGLIRVYADGHVERPPIIPNVPCSTTASNKGV 65

Query: 61  TAKDVLIDNFTNTWARIYVARSLQNLPLLVYFHGGGFCVGSATWSCYHEFLLDLASKAKC 120
           +AKDVLIDNFTNTWARIY+A S + LPLLVYFHGGGFCVGSA WSCYHEFLLDLASKAKC
Sbjct: 66  SAKDVLIDNFTNTWARIYIANSQRKLPLLVYFHGGGFCVGSAAWSCYHEFLLDLASKAKC 125

Query: 121 VIISVNYRLSPENRLPAAYEDGFNALMWLKQQAISDPIEHKWWLGNCNASNLFLAGDSAG 180
            I+S+NYRLSPENRLPAAYEDGFNALMWLKQQAIS+P EHKWWLGNCN SNLFLAGDSAG
Sbjct: 126 AILSINYRLSPENRLPAAYEDGFNALMWLKQQAISEPNEHKWWLGNCNPSNLFLAGDSAG 185

Query: 181 ANIAYNITARLGLNEPTILNPLNLKGIILIQPFFGGEARTKSEKLATQPSTSALTLPASD 240
           ANIAYNI  R GLNEP  L PL LKG+ILIQPFFGGE+RTKSEKLA QPSTSALTL  +D
Sbjct: 186 ANIAYNIATRPGLNEPATLKPLTLKGVILIQPFFGGESRTKSEKLAAQPSTSALTLATAD 245

Query: 241 TYWRLSLPPGANRDHPWSNPLVNGVAKLRDTKLPPTLVCISELDILLDRNLELCAAMAAA 300
           TYWRLSLPPGA+RDHPWSNPLVNG  KLRDTKLPP++VCISELDIL+DRNLE CAAMAAA
Sbjct: 246 TYWRLSLPPGASRDHPWSNPLVNGGTKLRDTKLPPSMVCISELDILVDRNLEFCAAMAAA 305

Query: 301 GKKMEKVISKEVGHSFQILNKDQLSILRTQEMMFSV 337
           GKK+E+VI K VGH FQILNKDQ+S +RTQEMM S+
Sbjct: 306 GKKVERVIFKGVGHGFQILNKDQISAIRTQEMMVSI 341

BLAST of Sgr012991 vs. NCBI nr
Match: XP_038904086.1 (probable carboxylesterase 17 [Benincasa hispida])

HSP 1 Score: 582.8 bits (1501), Expect = 2.2e-162
Identity = 284/337 (84.27%), Postives = 303/337 (89.91%), Query Frame = 0

Query: 1   MATISFDPRFKLQMPIPHLQRGAIAEEIEGLIRVYADGHVERSPIIPNVSCSTIASDKGV 60
           MATIS DPR KLQMP  H+Q  A  EEIEGLIRVYADGHVER PIIPNVSC+T ASDKGV
Sbjct: 17  MATISIDPRLKLQMPKLHIQNEATVEEIEGLIRVYADGHVERFPIIPNVSCNTTASDKGV 76

Query: 61  TAKDVLIDNFTNTWARIYVARSLQNLPLLVYFHGGGFCVGSATWSCYHEFLLDLASKAKC 120
           TAKDVLIDNFTNTWARIYVA S +NLPLLVYFHGGGFCVGSA WSCYHEFLLDLASKAKC
Sbjct: 77  TAKDVLIDNFTNTWARIYVANSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLDLASKAKC 136

Query: 121 VIISVNYRLSPENRLPAAYEDGFNALMWLKQQAISDPIEHKWWLGNCNASNLFLAGDSAG 180
           VI+SVNYRLSPENRLPAAYEDGFN LMWLKQ AI++PIEHKWWLGNCN S LFLAGDSAG
Sbjct: 137 VILSVNYRLSPENRLPAAYEDGFNTLMWLKQLAITNPIEHKWWLGNCNQSKLFLAGDSAG 196

Query: 181 ANIAYNITARLGLNEPTILNPLNLKGIILIQPFFGGEARTKSEKLATQPST-SALTLPAS 240
           ANIAYNI AR+GLNEPTIL PLNLKGIILIQPFFGGE+RTKSEKLATQPS+ SALTL A+
Sbjct: 197 ANIAYNIAARVGLNEPTILKPLNLKGIILIQPFFGGESRTKSEKLATQPSSASALTLDAA 256

Query: 241 DTYWRLSLPPGANRDHPWSNPLVNGVAKLRDTKLPPTLVCISELDILLDRNLELCAAMAA 300
           DTYWRLSLPPGANRDH WSNPLVNG  KLR+TK+PP +VCISELDIL+DRNLE CAAM A
Sbjct: 257 DTYWRLSLPPGANRDHTWSNPLVNGGVKLRETKVPPLMVCISELDILVDRNLEFCAAMTA 316

Query: 301 AGKKMEKVISKEVGHSFQILNKDQLSILRTQEMMFSV 337
           AGKK+E+VI K VGHSFQILNKDQ+S  R +EMM  +
Sbjct: 317 AGKKVERVILKGVGHSFQILNKDQISSARRREMMVCI 353

BLAST of Sgr012991 vs. NCBI nr
Match: XP_022998605.1 (probable carboxylesterase 17 [Cucurbita maxima])

HSP 1 Score: 564.7 bits (1454), Expect = 6.3e-157
Identity = 275/337 (81.60%), Postives = 297/337 (88.13%), Query Frame = 0

Query: 1   MATISFDPRFKLQMPIPHLQRGAIAEEIEGLIRVYADGHVERSPIIPNVSCSTIASDKGV 60
           MA +  DPR KLQM  PHLQ GA +EEIEGLIRVYADGHVER PIIPNV+CST AS+KGV
Sbjct: 1   MAAMYIDPRLKLQMAKPHLQNGATSEEIEGLIRVYADGHVERPPIIPNVACSTTASNKGV 60

Query: 61  TAKDVLIDNFTNTWARIYVARSLQNLPLLVYFHGGGFCVGSATWSCYHEFLLDLASKAKC 120
           TAKDVLID FTNTWARIY+  S +NLPLLVYFHGGGFCVGSA WSCYHEFLL+LASKAKC
Sbjct: 61  TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC 120

Query: 121 VIISVNYRLSPENRLPAAYEDGFNALMWLKQQAISDPIEHKWWLGNCNASNLFLAGDSAG 180
           VI+S+NYRLSPENRLPAAYEDGFNA+MWLKQQAI++PIEHKWWLGNCN S LFLAGDSAG
Sbjct: 121 VILSINYRLSPENRLPAAYEDGFNAVMWLKQQAITNPIEHKWWLGNCNPSKLFLAGDSAG 180

Query: 181 ANIAYNITARLGLNEPTILNPLNLKGIILIQPFFGGEARTKSEKLATQP-STSALTLPAS 240
           ANIAYN   RLG NE T+L P  LKGIILIQPFFGGE+RTKSEKLA  P STSALTL AS
Sbjct: 181 ANIAYNTATRLGHNESTLLKPPTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAS 240

Query: 241 DTYWRLSLPPGANRDHPWSNPLVNGVAKLRDTKLPPTLVCISELDILLDRNLELCAAMAA 300
           DTYWRLSLPP ANRDHPWSNPLVNG AKL++ KLPP +VCISELDILLDRNLE CAAMAA
Sbjct: 241 DTYWRLSLPPTANRDHPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAAMAA 300

Query: 301 AGKKMEKVISKEVGHSFQILNKDQLSILRTQEMMFSV 337
           AGKK+E+VI K VGH FQILNKDQ+S  RTQE+M S+
Sbjct: 301 AGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSI 337

BLAST of Sgr012991 vs. NCBI nr
Match: XP_023523365.1 (probable carboxylesterase 17 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 563.1 bits (1450), Expect = 1.8e-156
Identity = 273/337 (81.01%), Postives = 295/337 (87.54%), Query Frame = 0

Query: 1   MATISFDPRFKLQMPIPHLQRGAIAEEIEGLIRVYADGHVERSPIIPNVSCSTIASDKGV 60
           MA +  DPR KLQM  PHLQ G  +EEIEGLIRVYADGHVER PIIPNV+CST ASDKGV
Sbjct: 6   MAAMYVDPRLKLQMAKPHLQNGGTSEEIEGLIRVYADGHVERPPIIPNVACSTTASDKGV 65

Query: 61  TAKDVLIDNFTNTWARIYVARSLQNLPLLVYFHGGGFCVGSATWSCYHEFLLDLASKAKC 120
           TAKDVLID FTNTWARIY+  S +NLPLLVYFHGGGFCVGSA WSCYHEFLL+LASKAKC
Sbjct: 66  TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC 125

Query: 121 VIISVNYRLSPENRLPAAYEDGFNALMWLKQQAISDPIEHKWWLGNCNASNLFLAGDSAG 180
           VI+S+NYRLSPENRLPAAY+DGFNALMWLKQQAI++PIEHKWW GNCN S LFLAGDSAG
Sbjct: 126 VILSINYRLSPENRLPAAYDDGFNALMWLKQQAITNPIEHKWWSGNCNPSKLFLAGDSAG 185

Query: 181 ANIAYNITARLGLNEPTILNPLNLKGIILIQPFFGGEARTKSEKLATQP-STSALTLPAS 240
           ANI YN   RLG NE T+L PL LKGIILIQPFFGGE+RTKSEKLA  P STSALTL AS
Sbjct: 186 ANIVYNTATRLGHNESTLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAS 245

Query: 241 DTYWRLSLPPGANRDHPWSNPLVNGVAKLRDTKLPPTLVCISELDILLDRNLELCAAMAA 300
           DTYWRLSLPP ANRDHPWSNPLVNG AKL++TKLPP +VCISELDILLDRNLE CAAMA 
Sbjct: 246 DTYWRLSLPPTANRDHPWSNPLVNGGAKLKETKLPPLMVCISELDILLDRNLEFCAAMAG 305

Query: 301 AGKKMEKVISKEVGHSFQILNKDQLSILRTQEMMFSV 337
           AGKK+++VI K VGH FQILNKDQ+S  RTQE+M S+
Sbjct: 306 AGKKVDRVILKGVGHGFQILNKDQISTQRTQELMGSI 342

BLAST of Sgr012991 vs. NCBI nr
Match: XP_022948283.1 (probable carboxylesterase 17 [Cucurbita moschata])

HSP 1 Score: 563.1 bits (1450), Expect = 1.8e-156
Identity = 272/337 (80.71%), Postives = 296/337 (87.83%), Query Frame = 0

Query: 1   MATISFDPRFKLQMPIPHLQRGAIAEEIEGLIRVYADGHVERSPIIPNVSCSTIASDKGV 60
           MA +  DPR KLQM  PHLQ G  +EEIEGLIRVYADGHVER PIIPNV+CST ASDKGV
Sbjct: 6   MAAMYIDPRLKLQMAKPHLQNGGTSEEIEGLIRVYADGHVERPPIIPNVACSTTASDKGV 65

Query: 61  TAKDVLIDNFTNTWARIYVARSLQNLPLLVYFHGGGFCVGSATWSCYHEFLLDLASKAKC 120
           TAKDVLID FTNTWARIY+  S +NLPLLVYFHGGGFCVGSA WSCYHEFLL+LASKAKC
Sbjct: 66  TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC 125

Query: 121 VIISVNYRLSPENRLPAAYEDGFNALMWLKQQAISDPIEHKWWLGNCNASNLFLAGDSAG 180
           VI+S+NYRLSPENRLPAAY+DGFNALMWLKQQAI++PIEHKWWLGNCN S LFLAGDSAG
Sbjct: 126 VILSINYRLSPENRLPAAYDDGFNALMWLKQQAITNPIEHKWWLGNCNPSKLFLAGDSAG 185

Query: 181 ANIAYNITARLGLNEPTILNPLNLKGIILIQPFFGGEARTKSEKLATQP-STSALTLPAS 240
           ANI+YN   R+G NE T+L PL LKGIILIQPFFGGE+RTKSEKLA  P STSALTL A+
Sbjct: 186 ANISYNTATRIGHNESTLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAA 245

Query: 241 DTYWRLSLPPGANRDHPWSNPLVNGVAKLRDTKLPPTLVCISELDILLDRNLELCAAMAA 300
           DTYWRLSLPP ANRDHPWSNPLVNG AKL++ KLPP +VCISELDILLDRNLE CAAMA 
Sbjct: 246 DTYWRLSLPPTANRDHPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAAMAG 305

Query: 301 AGKKMEKVISKEVGHSFQILNKDQLSILRTQEMMFSV 337
           AGKK+E+VI K VGH FQILNKDQ+S  RTQE+M S+
Sbjct: 306 AGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSI 342

BLAST of Sgr012991 vs. ExPASy Swiss-Prot
Match: Q9LFR7 (Probable carboxylesterase 17 OS=Arabidopsis thaliana OX=3702 GN=CXE17 PE=1 SV=1)

HSP 1 Score: 344.0 bits (881), Expect = 2.3e-93
Identity = 182/342 (53.22%), Postives = 228/342 (66.67%), Query Frame = 0

Query: 1   MATISFDPRFK----LQMPIPHLQRGAIAEEIEGLIRVYADGHVERSPIIPNVSCSTIAS 60
           MATISF    +     +    H + G + EEIEGLI+V+ DG VER PI+P VS  TI  
Sbjct: 1   MATISFSHNHQSSDNRRGGSHHHRHGPVVEEIEGLIKVFNDGCVERPPIVPIVS-PTIHP 60

Query: 61  DKGVTAKDVLIDNFTNTWARIYV-----ARSLQNLPLLVYFHGGGFCVGSATWSCYHEFL 120
               TA D+ + N  +TW R+Y+     A     LPLLVYFHGGGFCVGSA WSCYH+FL
Sbjct: 61  SSKATAFDIKLSN--DTWTRVYIPDAAAASPSVTLPLLVYFHGGGFCVGSAAWSCYHDFL 120

Query: 121 LDLASKAKCVIISVNYRLSPENRLPAAYEDGFNALMWLKQQAISDPIEHKWWLGNCNASN 180
             LA KA+CVI+SVNYRL+PE+RLPAAY+DG N + WL +Q IS    +  WL  CN SN
Sbjct: 121 TSLAVKARCVIVSVNYRLAPEHRLPAAYDDGVNVVSWLVKQQISTGGGYPSWLSKCNLSN 180

Query: 181 LFLAGDSAGANIAYNITARLGLNEPTILNPLNLKGIILIQPFFGGEARTKSEKLATQPST 240
           +FLAGDSAGANIAY +  R+ +      N L+LKGIILI PFFGGE+RT SEK      +
Sbjct: 181 VFLAGDSAGANIAYQVAVRI-MASGKYANTLHLKGIILIHPFFGGESRTSSEKQQHHTKS 240

Query: 241 SALTLPASDTYWRLSLPPGANRDHPWSNPLVNGVAKLRDTKLPPTLVCISELDILLDRNL 300
           SALTL ASD YWRL+LP GA+RDHPW NPL++        KLP T+V ++E DIL +RNL
Sbjct: 241 SALTLSASDAYWRLALPRGASRDHPWCNPLMSSAG----AKLPTTMVFMAEFDILKERNL 300

Query: 301 ELCAAMAAAGKKMEKVISKEVGHSFQILNKDQLSILRTQEMM 334
           E+C  M + GK++E ++   VGH+F IL+   +S  R  +MM
Sbjct: 301 EMCKVMRSHGKRVEGIVHGGVGHAFHILDNSSVSRDRIHDMM 334

BLAST of Sgr012991 vs. ExPASy Swiss-Prot
Match: Q9SX25 (Probable carboxylesterase 6 OS=Arabidopsis thaliana OX=3702 GN=CXE6 PE=2 SV=1)

HSP 1 Score: 306.6 bits (784), Expect = 4.1e-82
Identity = 155/320 (48.44%), Postives = 216/320 (67.50%), Query Frame = 0

Query: 22  GAIAEEIEGLIRVYADGHVERSPIIPNVSCSTIASDKGVTAKDVLIDNFTNTWARIYV-- 81
           G + +E+EGLI+VY DGHVERS ++P V  S +  + GVT  DV+ID  TN WAR+YV  
Sbjct: 21  GPVVDEVEGLIKVYKDGHVERSQLLPCVDPS-LPLELGVTCSDVVIDKLTNVWARLYVPM 80

Query: 82  ---ARSLQNLPLLVYFHGGGFCVGSATWSCYHEFLLDLASKAKCVIISVNYRLSPENRLP 141
                S+  LPL+VYFHGGGFCVGSA+W CYHEFL  L+++++C+++SVNYRL+PEN LP
Sbjct: 81  TTTKSSVSKLPLIVYFHGGGFCVGSASWLCYHEFLARLSARSRCLVMSVNYRLAPENPLP 140

Query: 142 AAYEDGFNALMWLKQQAISDPIEHKWWLGNCNASNLFLAGDSAGANIAYNITARLGLNEP 201
           AAYEDG NA++WL  +A +D +    W   C+   +FLAGDSAG NIA  + ARL   E 
Sbjct: 141 AAYEDGVNAILWL-NKARNDNL----WAKQCDFGRIFLAGDSAGGNIAQQVAARLASPED 200

Query: 202 TILNPLNLKGIILIQPFFGGEARTKSEKLATQPSTSALTLPASDTYWRLSLPPGANRDHP 261
                L ++G ILIQPF+ GE RT+SE+      T+ LTL +SD +WR+SLP GANR+HP
Sbjct: 201 L---ALKIEGTILIQPFYSGEERTESERRVGNDKTAVLTLASSDAWWRMSLPRGANREHP 260

Query: 262 WSNPLVNGVAKLRDTKLPPTLVCISELDILLDRNLELCAAMAAAGKKMEKVISKEVGHSF 321
           +  P+      ++ + +  TLVC++E+D+L+D N+E+C         +++V+ K VGH+F
Sbjct: 261 YCKPV---KMIIKSSTVTRTLVCVAEMDLLMDSNMEMCDGNEDV---IKRVLHKGVGHAF 320

Query: 322 QILNKDQLSILRTQEMMFSV 337
            IL K QL+   T EM+  +
Sbjct: 321 HILGKSQLAHTTTLEMLCQI 325

BLAST of Sgr012991 vs. ExPASy Swiss-Prot
Match: Q9FG13 (Probable carboxylesterase 15 OS=Arabidopsis thaliana OX=3702 GN=CXE15 PE=2 SV=1)

HSP 1 Score: 195.7 bits (496), Expect = 1.0e-48
Identity = 113/299 (37.79%), Postives = 168/299 (56.19%), Query Frame = 0

Query: 24  IAEEIEGLIRVYADGHVERSPIIPNVSCS-TIASDKGVTAKDVLIDNFTNTWARIYVARS 83
           +AE+  GL+++ ++G V RS  I  ++      +++ V  KD +     N   R+Y   S
Sbjct: 10  VAEDCMGLLQLLSNGTVLRSESIDLITQQIPFKNNQTVLFKDSIYHKPNNLHLRLYKPIS 69

Query: 84  LQN---LPLLVYFHGGGFCVGSATWSCYHEFLLDLASKAKCVIISVNYRLSPENRLPAAY 143
             N   LP++V+FHGGGFC GS +W  +H F L LAS    +++S +YRL+PE+RLPAA+
Sbjct: 70  ASNRTALPVVVFFHGGGFCFGSRSWPHFHNFCLTLASSLNALVVSPDYRLAPEHRLPAAF 129

Query: 144 EDGFNALMWLKQQAISDPIEHKWWLG-NCNASNLFLAGDSAGANIAYNITARLGLNEPTI 203
           ED    L WL  QA+SD + H +  G + +   +F+ GDS+G NIA+ +  R G +    
Sbjct: 130 EDAEAVLTWLWDQAVSDGVNHWFEDGTDVDFDRVFVVGDSSGGNIAHQLAVRFG-SGSIE 189

Query: 204 LNPLNLKGIILIQPFFGGEARTKSEKLATQPSTSALTLPASDTYWRLSLPPGANRDHPWS 263
           L P+ ++G +L+ PFFGGE RT SE     PS + L+L   D +WRLSLP GA RDH  +
Sbjct: 190 LTPVRVRGYVLMGPFFGGEERTNSE---NGPSEALLSLDLLDKFWRLSLPNGATRDHHMA 249

Query: 264 NPLVNGVAKLRDTKLPPTLVCISELDILLDRNLELCAAM-AAAGKKMEKVISKEVGHSF 317
           NP       L    L P LV +   ++L DR  E    +    GK+++ +  +   H F
Sbjct: 250 NPFGPTSPTLESISLEPMLVIVGGSELLRDRAKEYAYKLKKMGGKRVDYIEFENKEHGF 304

BLAST of Sgr012991 vs. ExPASy Swiss-Prot
Match: Q9LVB8 (Probable carboxylesterase 120 OS=Arabidopsis thaliana OX=3702 GN=CXE20 PE=2 SV=1)

HSP 1 Score: 166.8 bits (421), Expect = 5.0e-40
Identity = 92/246 (37.40%), Postives = 140/246 (56.91%), Query Frame = 0

Query: 37  DGHVERSPIIPNVSCSTIASDKG----VTAKDVLIDNFTNTWARIYVARSL--------Q 96
           DG + R   + N  C+    D        +KD+ ++   +TW R+Y+  S         Q
Sbjct: 21  DGSITRD--LSNFPCTAATPDPSPLNPAVSKDLPVNQLKSTWLRLYLPSSAVNEGNVSSQ 80

Query: 97  NLPLLVYFHGGGFCVGSATWSCYHEFLLDLASKAKCVIISVNYRLSPENRLPAAYEDGFN 156
            LP++VY+HGGGF + S     +H+F  ++A     +++S +YRL+PE+RLPAAY+DG  
Sbjct: 81  KLPIVVYYHGGGFILCSVDMQLFHDFCSEVARDLNAIVVSPSYRLAPEHRLPAAYDDGVE 140

Query: 157 ALMWLKQQAISDPIEHKWWLGNCNASNLFLAGDSAGANIAYNITARLGLNEPTILNPLNL 216
           AL W+K        + +W   + + SN+FL G SAG N+AYN+  R  ++  + L+PL +
Sbjct: 141 ALDWIKTS------DDEWIKSHADFSNVFLMGTSAGGNLAYNVGLR-SVDSVSDLSPLQI 200

Query: 217 KGIILIQPFFGGEARTKSE-KLATQPSTSALTLPASDTYWRLSLPPGANRDHPWSNPLV- 269
           +G+IL  PFFGGE R++SE +L        +    +D  W LSLP G +RDH +SNP V 
Sbjct: 201 RGLILHHPFFGGEERSESEIRLMNDQVCPPI---VTDVMWDLSLPVGVDRDHEYSNPTVG 254

BLAST of Sgr012991 vs. ExPASy Swiss-Prot
Match: O64640 (Probable carboxylesterase 8 OS=Arabidopsis thaliana OX=3702 GN=CXE8 PE=2 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 2.5e-39
Identity = 101/294 (34.35%), Postives = 158/294 (53.74%), Query Frame = 0

Query: 62  AKDVLIDNFTNTWARIYVARSL---QNLPLLVYFHGGGFCVGSATWSCYHEFLLDLASKA 121
           +KD+ ++   NT+ RI+  R++     LP+LVYFHGGGF + SA  + +HE    +A + 
Sbjct: 39  SKDIPLNQTNNTFIRIFKPRNIPPESKLPILVYFHGGGFILYSAASAPFHESCTKMADRL 98

Query: 122 KCVIISVNYRLSPENRLPAAYEDGFNALMWLKQQAISDPIE----HKWWLGNCNASNLFL 181
           + +I+SV YRL+PE+RLPAAYED   A++WL+ QA   PI       W     + S  ++
Sbjct: 99  QTIILSVEYRLAPEHRLPAAYEDAVEAILWLRDQA-RGPINGGDCDTWLKDGVDFSKCYV 158

Query: 182 AGDSAGANIAYNITARLGLNEPTILNPLNLKGIILIQPFFGG------EARTKSEKLATQ 241
            G S+G NI YN+  R+     T L+P+ ++G+I+ Q FFGG      E+R K +K+   
Sbjct: 159 MGSSSGGNIVYNVALRV---VDTDLSPVKIQGLIMNQAFFGGVEPSDSESRLKDDKIC-- 218

Query: 242 PSTSALTLPASDTYWRLSLPPGANRDHPWSNPLVNGVAKLRDT--KLPPTLVCISELDIL 301
                  LPA+   W L LP G +RDH +SNP+ +   + +D   + P TL+     D L
Sbjct: 219 ------PLPATHLLWSLCLPDGVDRDHVYSNPIKSSGPQEKDKMGRFPSTLINGYGGDPL 278

Query: 302 LDRNLELCAAMAAAGKKMEKVISKEVGHSFQILNKDQLSIL-RTQEMMFSVASS 340
           +DR   +   +   G  +E    K+  H+ ++ + ++   L  T E      SS
Sbjct: 279 VDRQRHVAEMLKGRGVHVETRFDKDGFHACELFDGNKAKALYETVEAFMKSCSS 320

BLAST of Sgr012991 vs. ExPASy TrEMBL
Match: A0A6J1DL90 (probable carboxylesterase 17 OS=Momordica charantia OX=3673 GN=LOC111022166 PE=4 SV=1)

HSP 1 Score: 584.3 bits (1505), Expect = 3.7e-163
Identity = 280/336 (83.33%), Postives = 300/336 (89.29%), Query Frame = 0

Query: 1   MATISFDPRFKLQMPIPHLQRGAIAEEIEGLIRVYADGHVERSPIIPNVSCSTIASDKGV 60
           MA IS DPR KLQMP  HLQ  + AEEIEGLIRVYADGHVER PIIPNV CST AS+KGV
Sbjct: 6   MAAISIDPRLKLQMPKSHLQCASTAEEIEGLIRVYADGHVERPPIIPNVPCSTTASNKGV 65

Query: 61  TAKDVLIDNFTNTWARIYVARSLQNLPLLVYFHGGGFCVGSATWSCYHEFLLDLASKAKC 120
           +AKDVLIDNFTNTWARIY+A S + LPLLVYFHGGGFCVGSA WSCYHEFLLDLASKAKC
Sbjct: 66  SAKDVLIDNFTNTWARIYIANSQRKLPLLVYFHGGGFCVGSAAWSCYHEFLLDLASKAKC 125

Query: 121 VIISVNYRLSPENRLPAAYEDGFNALMWLKQQAISDPIEHKWWLGNCNASNLFLAGDSAG 180
            I+S+NYRLSPENRLPAAYEDGFNALMWLKQQAIS+P EHKWWLGNCN SNLFLAGDSAG
Sbjct: 126 AILSINYRLSPENRLPAAYEDGFNALMWLKQQAISEPNEHKWWLGNCNPSNLFLAGDSAG 185

Query: 181 ANIAYNITARLGLNEPTILNPLNLKGIILIQPFFGGEARTKSEKLATQPSTSALTLPASD 240
           ANIAYNI  R GLNEP  L PL LKG+ILIQPFFGGE+RTKSEKLA QPSTSALTL  +D
Sbjct: 186 ANIAYNIATRPGLNEPATLKPLTLKGVILIQPFFGGESRTKSEKLAAQPSTSALTLATAD 245

Query: 241 TYWRLSLPPGANRDHPWSNPLVNGVAKLRDTKLPPTLVCISELDILLDRNLELCAAMAAA 300
           TYWRLSLPPGA+RDHPWSNPLVNG  KLRDTKLPP++VCISELDIL+DRNLE CAAMAAA
Sbjct: 246 TYWRLSLPPGASRDHPWSNPLVNGGTKLRDTKLPPSMVCISELDILVDRNLEFCAAMAAA 305

Query: 301 GKKMEKVISKEVGHSFQILNKDQLSILRTQEMMFSV 337
           GKK+E+VI K VGH FQILNKDQ+S +RTQEMM S+
Sbjct: 306 GKKVERVIFKGVGHGFQILNKDQISAIRTQEMMVSI 341

BLAST of Sgr012991 vs. ExPASy TrEMBL
Match: A0A6J1KCZ3 (probable carboxylesterase 17 OS=Cucurbita maxima OX=3661 GN=LOC111493192 PE=4 SV=1)

HSP 1 Score: 564.7 bits (1454), Expect = 3.1e-157
Identity = 275/337 (81.60%), Postives = 297/337 (88.13%), Query Frame = 0

Query: 1   MATISFDPRFKLQMPIPHLQRGAIAEEIEGLIRVYADGHVERSPIIPNVSCSTIASDKGV 60
           MA +  DPR KLQM  PHLQ GA +EEIEGLIRVYADGHVER PIIPNV+CST AS+KGV
Sbjct: 1   MAAMYIDPRLKLQMAKPHLQNGATSEEIEGLIRVYADGHVERPPIIPNVACSTTASNKGV 60

Query: 61  TAKDVLIDNFTNTWARIYVARSLQNLPLLVYFHGGGFCVGSATWSCYHEFLLDLASKAKC 120
           TAKDVLID FTNTWARIY+  S +NLPLLVYFHGGGFCVGSA WSCYHEFLL+LASKAKC
Sbjct: 61  TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC 120

Query: 121 VIISVNYRLSPENRLPAAYEDGFNALMWLKQQAISDPIEHKWWLGNCNASNLFLAGDSAG 180
           VI+S+NYRLSPENRLPAAYEDGFNA+MWLKQQAI++PIEHKWWLGNCN S LFLAGDSAG
Sbjct: 121 VILSINYRLSPENRLPAAYEDGFNAVMWLKQQAITNPIEHKWWLGNCNPSKLFLAGDSAG 180

Query: 181 ANIAYNITARLGLNEPTILNPLNLKGIILIQPFFGGEARTKSEKLATQP-STSALTLPAS 240
           ANIAYN   RLG NE T+L P  LKGIILIQPFFGGE+RTKSEKLA  P STSALTL AS
Sbjct: 181 ANIAYNTATRLGHNESTLLKPPTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAS 240

Query: 241 DTYWRLSLPPGANRDHPWSNPLVNGVAKLRDTKLPPTLVCISELDILLDRNLELCAAMAA 300
           DTYWRLSLPP ANRDHPWSNPLVNG AKL++ KLPP +VCISELDILLDRNLE CAAMAA
Sbjct: 241 DTYWRLSLPPTANRDHPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAAMAA 300

Query: 301 AGKKMEKVISKEVGHSFQILNKDQLSILRTQEMMFSV 337
           AGKK+E+VI K VGH FQILNKDQ+S  RTQE+M S+
Sbjct: 301 AGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSI 337

BLAST of Sgr012991 vs. ExPASy TrEMBL
Match: A0A6J1G8X8 (probable carboxylesterase 17 OS=Cucurbita moschata OX=3662 GN=LOC111452001 PE=4 SV=1)

HSP 1 Score: 563.1 bits (1450), Expect = 8.9e-157
Identity = 272/337 (80.71%), Postives = 296/337 (87.83%), Query Frame = 0

Query: 1   MATISFDPRFKLQMPIPHLQRGAIAEEIEGLIRVYADGHVERSPIIPNVSCSTIASDKGV 60
           MA +  DPR KLQM  PHLQ G  +EEIEGLIRVYADGHVER PIIPNV+CST ASDKGV
Sbjct: 6   MAAMYIDPRLKLQMAKPHLQNGGTSEEIEGLIRVYADGHVERPPIIPNVACSTTASDKGV 65

Query: 61  TAKDVLIDNFTNTWARIYVARSLQNLPLLVYFHGGGFCVGSATWSCYHEFLLDLASKAKC 120
           TAKDVLID FTNTWARIY+  S +NLPLLVYFHGGGFCVGSA WSCYHEFLL+LASKAKC
Sbjct: 66  TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC 125

Query: 121 VIISVNYRLSPENRLPAAYEDGFNALMWLKQQAISDPIEHKWWLGNCNASNLFLAGDSAG 180
           VI+S+NYRLSPENRLPAAY+DGFNALMWLKQQAI++PIEHKWWLGNCN S LFLAGDSAG
Sbjct: 126 VILSINYRLSPENRLPAAYDDGFNALMWLKQQAITNPIEHKWWLGNCNPSKLFLAGDSAG 185

Query: 181 ANIAYNITARLGLNEPTILNPLNLKGIILIQPFFGGEARTKSEKLATQP-STSALTLPAS 240
           ANI+YN   R+G NE T+L PL LKGIILIQPFFGGE+RTKSEKLA  P STSALTL A+
Sbjct: 186 ANISYNTATRIGHNESTLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAA 245

Query: 241 DTYWRLSLPPGANRDHPWSNPLVNGVAKLRDTKLPPTLVCISELDILLDRNLELCAAMAA 300
           DTYWRLSLPP ANRDHPWSNPLVNG AKL++ KLPP +VCISELDILLDRNLE CAAMA 
Sbjct: 246 DTYWRLSLPPTANRDHPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAAMAG 305

Query: 301 AGKKMEKVISKEVGHSFQILNKDQLSILRTQEMMFSV 337
           AGKK+E+VI K VGH FQILNKDQ+S  RTQE+M S+
Sbjct: 306 AGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSI 342

BLAST of Sgr012991 vs. ExPASy TrEMBL
Match: A0A2N9EQ59 (Abhydrolase_3 domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS4850 PE=4 SV=1)

HSP 1 Score: 459.5 bits (1181), Expect = 1.4e-125
Identity = 226/336 (67.26%), Postives = 261/336 (77.68%), Query Frame = 0

Query: 1   MATISFDPRFKLQMPIPHLQRGAIAEEIEGLIRVYADGHVERSPIIPNVSCSTIASDKGV 60
           MA IS DPRFKLQ+   H Q G + EEIEGLI+V  DGHVER PI+P V C T+A ++GV
Sbjct: 1   MAAISLDPRFKLQVGKNHHQHGGVVEEIEGLIKVCMDGHVERPPIVPIVPC-TMALERGV 60

Query: 61  TAKDVLIDNFTNTWARIYVARSLQNLPLLVYFHGGGFCVGSATWSCYHEFLLDLASKAKC 120
           TA DV+ID  T+ WARIYV      LPLLVYFHGGGFCVGSA WSCY+EFL +LASKA C
Sbjct: 61  TANDVVIDKCTSLWARIYVPSCSGKLPLLVYFHGGGFCVGSAAWSCYNEFLSNLASKANC 120

Query: 121 VIISVNYRLSPENRLPAAYEDGFNALMWLKQQAISDPIEHKWWLGNCNASNLFLAGDSAG 180
           VIISVNYRL+PENRLPAAYEDGFN LMW+KQQA++   EHKWWL +CN +++FL GDS+G
Sbjct: 121 VIISVNYRLAPENRLPAAYEDGFNTLMWVKQQALNGSNEHKWWLRHCNLTSMFLGGDSSG 180

Query: 181 ANIAYNITARL---GLNEPTILNPLNLKGIILIQPFFGGEARTKSEKLATQPSTSALTLP 240
           ANIAYN+  RL   G +E TI+ PL LKG ILIQPFFGGEART SEK   QP+ S LTL 
Sbjct: 181 ANIAYNVATRLGSSGTSESTIIRPLCLKGTILIQPFFGGEARTWSEKYTNQPANSGLTLL 240

Query: 241 ASDTYWRLSLPPGANRDHPWSNPLVNGVAKLRDTKLPPTLVCISELDILLDRNLELCAAM 300
            SDTYWRLSLP G NRD+PW NPL NG+AKLR+ +LP  +VC+SE DIL DRNLE C A+
Sbjct: 241 VSDTYWRLSLPIGTNRDNPWCNPLANGMAKLREFRLPSIMVCVSETDILKDRNLEFCTAL 300

Query: 301 AAAGKKMEKVISKEVGHSFQILNKDQLSILRTQEMM 334
             AGK++E VI K VGH+FQIL+  QLS  RT EMM
Sbjct: 301 TNAGKRVETVIYKGVGHAFQILHNSQLSQPRTHEMM 335

BLAST of Sgr012991 vs. ExPASy TrEMBL
Match: A0A6P5SNC8 (probable carboxylesterase 17 OS=Prunus avium OX=42229 GN=LOC110758250 PE=4 SV=1)

HSP 1 Score: 459.1 bits (1180), Expect = 1.8e-125
Identity = 222/334 (66.47%), Postives = 264/334 (79.04%), Query Frame = 0

Query: 1   MATISFDPRFKLQ-MPIPHLQRGAIAEEIEGLIRVYADGHVERSPIIPNVSCSTIASDKG 60
           M+TIS DPR  LQ +  P  Q G + EEIEGL+RV+ DG++ER PIIP+V  ST+A   G
Sbjct: 6   MSTISLDPRLNLQAIKNPPNQHGVVLEEIEGLLRVHKDGNIERPPIIPSVP-STLALPHG 65

Query: 61  VTAKDVLIDNFTNTWARIYVARSLQNLPLLVYFHGGGFCVGSATWSCYHEFLLDLASKAK 120
           VTAKDV+ID FTN WARIYV     NLP+LVYFHGGGFCVGSA WSCYH FL +LA KA 
Sbjct: 66  VTAKDVVIDKFTNLWARIYVPSHPGNLPVLVYFHGGGFCVGSAAWSCYHNFLANLACKAS 125

Query: 121 CVIISVNYRLSPENRLPAAYEDGFNALMWLKQQAISDPIEHKWWLGNCNASNLFLAGDSA 180
           CVIISVNYRL+PENRLPAAY+DGF  LMW+KQQA+S+P E +WWL  C+ S+LF+ GDSA
Sbjct: 126 CVIISVNYRLAPENRLPAAYDDGFKTLMWVKQQALSEPSEQRWWLSRCSLSSLFIVGDSA 185

Query: 181 GANIAYNITARLGLNEPTILNPLNLKGIILIQPFFGGEARTKSEKLATQPSTSALTLPAS 240
           GANIAYN+T +L   +P+ L PL+LKG ILIQPFFGGEART SEK ATQP TSALTL  S
Sbjct: 186 GANIAYNVTTQLVPCDPSSLRPLSLKGTILIQPFFGGEARTWSEKYATQPPTSALTLSNS 245

Query: 241 DTYWRLSLPPGANRDHPWSNPLVNGVAKLRDTKLPPTLVCISELDILLDRNLELCAAMAA 300
           D YWRL+LP GAN+DHPW NPL NGV KLRD +LP  +VCISELDIL DRNLE+C A+++
Sbjct: 246 DVYWRLALPFGANKDHPWCNPLANGVTKLRDLRLPAIMVCISELDILKDRNLEVCNALSS 305

Query: 301 AGKKMEKVISKEVGHSFQILNKDQLSILRTQEMM 334
            GK++E  + K VGH+FQ++   QLS  RTQE++
Sbjct: 306 LGKRVETTMYKGVGHAFQVMQNSQLSYSRTQELI 338

BLAST of Sgr012991 vs. TAIR 10
Match: AT5G16080.1 (carboxyesterase 17 )

HSP 1 Score: 344.0 bits (881), Expect = 1.6e-94
Identity = 182/342 (53.22%), Postives = 228/342 (66.67%), Query Frame = 0

Query: 1   MATISFDPRFK----LQMPIPHLQRGAIAEEIEGLIRVYADGHVERSPIIPNVSCSTIAS 60
           MATISF    +     +    H + G + EEIEGLI+V+ DG VER PI+P VS  TI  
Sbjct: 1   MATISFSHNHQSSDNRRGGSHHHRHGPVVEEIEGLIKVFNDGCVERPPIVPIVS-PTIHP 60

Query: 61  DKGVTAKDVLIDNFTNTWARIYV-----ARSLQNLPLLVYFHGGGFCVGSATWSCYHEFL 120
               TA D+ + N  +TW R+Y+     A     LPLLVYFHGGGFCVGSA WSCYH+FL
Sbjct: 61  SSKATAFDIKLSN--DTWTRVYIPDAAAASPSVTLPLLVYFHGGGFCVGSAAWSCYHDFL 120

Query: 121 LDLASKAKCVIISVNYRLSPENRLPAAYEDGFNALMWLKQQAISDPIEHKWWLGNCNASN 180
             LA KA+CVI+SVNYRL+PE+RLPAAY+DG N + WL +Q IS    +  WL  CN SN
Sbjct: 121 TSLAVKARCVIVSVNYRLAPEHRLPAAYDDGVNVVSWLVKQQISTGGGYPSWLSKCNLSN 180

Query: 181 LFLAGDSAGANIAYNITARLGLNEPTILNPLNLKGIILIQPFFGGEARTKSEKLATQPST 240
           +FLAGDSAGANIAY +  R+ +      N L+LKGIILI PFFGGE+RT SEK      +
Sbjct: 181 VFLAGDSAGANIAYQVAVRI-MASGKYANTLHLKGIILIHPFFGGESRTSSEKQQHHTKS 240

Query: 241 SALTLPASDTYWRLSLPPGANRDHPWSNPLVNGVAKLRDTKLPPTLVCISELDILLDRNL 300
           SALTL ASD YWRL+LP GA+RDHPW NPL++        KLP T+V ++E DIL +RNL
Sbjct: 241 SALTLSASDAYWRLALPRGASRDHPWCNPLMSSAG----AKLPTTMVFMAEFDILKERNL 300

Query: 301 ELCAAMAAAGKKMEKVISKEVGHSFQILNKDQLSILRTQEMM 334
           E+C  M + GK++E ++   VGH+F IL+   +S  R  +MM
Sbjct: 301 EMCKVMRSHGKRVEGIVHGGVGHAFHILDNSSVSRDRIHDMM 334

BLAST of Sgr012991 vs. TAIR 10
Match: AT1G68620.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 306.6 bits (784), Expect = 2.9e-83
Identity = 155/320 (48.44%), Postives = 216/320 (67.50%), Query Frame = 0

Query: 22  GAIAEEIEGLIRVYADGHVERSPIIPNVSCSTIASDKGVTAKDVLIDNFTNTWARIYV-- 81
           G + +E+EGLI+VY DGHVERS ++P V  S +  + GVT  DV+ID  TN WAR+YV  
Sbjct: 21  GPVVDEVEGLIKVYKDGHVERSQLLPCVDPS-LPLELGVTCSDVVIDKLTNVWARLYVPM 80

Query: 82  ---ARSLQNLPLLVYFHGGGFCVGSATWSCYHEFLLDLASKAKCVIISVNYRLSPENRLP 141
                S+  LPL+VYFHGGGFCVGSA+W CYHEFL  L+++++C+++SVNYRL+PEN LP
Sbjct: 81  TTTKSSVSKLPLIVYFHGGGFCVGSASWLCYHEFLARLSARSRCLVMSVNYRLAPENPLP 140

Query: 142 AAYEDGFNALMWLKQQAISDPIEHKWWLGNCNASNLFLAGDSAGANIAYNITARLGLNEP 201
           AAYEDG NA++WL  +A +D +    W   C+   +FLAGDSAG NIA  + ARL   E 
Sbjct: 141 AAYEDGVNAILWL-NKARNDNL----WAKQCDFGRIFLAGDSAGGNIAQQVAARLASPED 200

Query: 202 TILNPLNLKGIILIQPFFGGEARTKSEKLATQPSTSALTLPASDTYWRLSLPPGANRDHP 261
                L ++G ILIQPF+ GE RT+SE+      T+ LTL +SD +WR+SLP GANR+HP
Sbjct: 201 L---ALKIEGTILIQPFYSGEERTESERRVGNDKTAVLTLASSDAWWRMSLPRGANREHP 260

Query: 262 WSNPLVNGVAKLRDTKLPPTLVCISELDILLDRNLELCAAMAAAGKKMEKVISKEVGHSF 321
           +  P+      ++ + +  TLVC++E+D+L+D N+E+C         +++V+ K VGH+F
Sbjct: 261 YCKPV---KMIIKSSTVTRTLVCVAEMDLLMDSNMEMCDGNEDV---IKRVLHKGVGHAF 320

Query: 322 QILNKDQLSILRTQEMMFSV 337
            IL K QL+   T EM+  +
Sbjct: 321 HILGKSQLAHTTTLEMLCQI 325

BLAST of Sgr012991 vs. TAIR 10
Match: AT5G06570.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 195.7 bits (496), Expect = 7.2e-50
Identity = 113/299 (37.79%), Postives = 168/299 (56.19%), Query Frame = 0

Query: 24  IAEEIEGLIRVYADGHVERSPIIPNVSCS-TIASDKGVTAKDVLIDNFTNTWARIYVARS 83
           +AE+  GL+++ ++G V RS  I  ++      +++ V  KD +     N   R+Y   S
Sbjct: 10  VAEDCMGLLQLLSNGTVLRSESIDLITQQIPFKNNQTVLFKDSIYHKPNNLHLRLYKPIS 69

Query: 84  LQN---LPLLVYFHGGGFCVGSATWSCYHEFLLDLASKAKCVIISVNYRLSPENRLPAAY 143
             N   LP++V+FHGGGFC GS +W  +H F L LAS    +++S +YRL+PE+RLPAA+
Sbjct: 70  ASNRTALPVVVFFHGGGFCFGSRSWPHFHNFCLTLASSLNALVVSPDYRLAPEHRLPAAF 129

Query: 144 EDGFNALMWLKQQAISDPIEHKWWLG-NCNASNLFLAGDSAGANIAYNITARLGLNEPTI 203
           ED    L WL  QA+SD + H +  G + +   +F+ GDS+G NIA+ +  R G +    
Sbjct: 130 EDAEAVLTWLWDQAVSDGVNHWFEDGTDVDFDRVFVVGDSSGGNIAHQLAVRFG-SGSIE 189

Query: 204 LNPLNLKGIILIQPFFGGEARTKSEKLATQPSTSALTLPASDTYWRLSLPPGANRDHPWS 263
           L P+ ++G +L+ PFFGGE RT SE     PS + L+L   D +WRLSLP GA RDH  +
Sbjct: 190 LTPVRVRGYVLMGPFFGGEERTNSE---NGPSEALLSLDLLDKFWRLSLPNGATRDHHMA 249

Query: 264 NPLVNGVAKLRDTKLPPTLVCISELDILLDRNLELCAAM-AAAGKKMEKVISKEVGHSF 317
           NP       L    L P LV +   ++L DR  E    +    GK+++ +  +   H F
Sbjct: 250 NPFGPTSPTLESISLEPMLVIVGGSELLRDRAKEYAYKLKKMGGKRVDYIEFENKEHGF 304

BLAST of Sgr012991 vs. TAIR 10
Match: AT5G06570.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 195.7 bits (496), Expect = 7.2e-50
Identity = 113/299 (37.79%), Postives = 168/299 (56.19%), Query Frame = 0

Query: 24  IAEEIEGLIRVYADGHVERSPIIPNVSCS-TIASDKGVTAKDVLIDNFTNTWARIYVARS 83
           +AE+  GL+++ ++G V RS  I  ++      +++ V  KD +     N   R+Y   S
Sbjct: 10  VAEDCMGLLQLLSNGTVLRSESIDLITQQIPFKNNQTVLFKDSIYHKPNNLHLRLYKPIS 69

Query: 84  LQN---LPLLVYFHGGGFCVGSATWSCYHEFLLDLASKAKCVIISVNYRLSPENRLPAAY 143
             N   LP++V+FHGGGFC GS +W  +H F L LAS    +++S +YRL+PE+RLPAA+
Sbjct: 70  ASNRTALPVVVFFHGGGFCFGSRSWPHFHNFCLTLASSLNALVVSPDYRLAPEHRLPAAF 129

Query: 144 EDGFNALMWLKQQAISDPIEHKWWLG-NCNASNLFLAGDSAGANIAYNITARLGLNEPTI 203
           ED    L WL  QA+SD + H +  G + +   +F+ GDS+G NIA+ +  R G +    
Sbjct: 130 EDAEAVLTWLWDQAVSDGVNHWFEDGTDVDFDRVFVVGDSSGGNIAHQLAVRFG-SGSIE 189

Query: 204 LNPLNLKGIILIQPFFGGEARTKSEKLATQPSTSALTLPASDTYWRLSLPPGANRDHPWS 263
           L P+ ++G +L+ PFFGGE RT SE     PS + L+L   D +WRLSLP GA RDH  +
Sbjct: 190 LTPVRVRGYVLMGPFFGGEERTNSE---NGPSEALLSLDLLDKFWRLSLPNGATRDHHMA 249

Query: 264 NPLVNGVAKLRDTKLPPTLVCISELDILLDRNLELCAAM-AAAGKKMEKVISKEVGHSF 317
           NP       L    L P LV +   ++L DR  E    +    GK+++ +  +   H F
Sbjct: 250 NPFGPTSPTLESISLEPMLVIVGGSELLRDRAKEYAYKLKKMGGKRVDYIEFENKEHGF 304

BLAST of Sgr012991 vs. TAIR 10
Match: AT5G62180.1 (carboxyesterase 20 )

HSP 1 Score: 166.8 bits (421), Expect = 3.6e-41
Identity = 92/246 (37.40%), Postives = 140/246 (56.91%), Query Frame = 0

Query: 37  DGHVERSPIIPNVSCSTIASDKG----VTAKDVLIDNFTNTWARIYVARSL--------Q 96
           DG + R   + N  C+    D        +KD+ ++   +TW R+Y+  S         Q
Sbjct: 21  DGSITRD--LSNFPCTAATPDPSPLNPAVSKDLPVNQLKSTWLRLYLPSSAVNEGNVSSQ 80

Query: 97  NLPLLVYFHGGGFCVGSATWSCYHEFLLDLASKAKCVIISVNYRLSPENRLPAAYEDGFN 156
            LP++VY+HGGGF + S     +H+F  ++A     +++S +YRL+PE+RLPAAY+DG  
Sbjct: 81  KLPIVVYYHGGGFILCSVDMQLFHDFCSEVARDLNAIVVSPSYRLAPEHRLPAAYDDGVE 140

Query: 157 ALMWLKQQAISDPIEHKWWLGNCNASNLFLAGDSAGANIAYNITARLGLNEPTILNPLNL 216
           AL W+K        + +W   + + SN+FL G SAG N+AYN+  R  ++  + L+PL +
Sbjct: 141 ALDWIKTS------DDEWIKSHADFSNVFLMGTSAGGNLAYNVGLR-SVDSVSDLSPLQI 200

Query: 217 KGIILIQPFFGGEARTKSE-KLATQPSTSALTLPASDTYWRLSLPPGANRDHPWSNPLV- 269
           +G+IL  PFFGGE R++SE +L        +    +D  W LSLP G +RDH +SNP V 
Sbjct: 201 RGLILHHPFFGGEERSESEIRLMNDQVCPPI---VTDVMWDLSLPVGVDRDHEYSNPTVG 254