Sgr012799 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr012799 vs. NCBI nr
Match: XP_038895570.1 (berberine bridge enzyme-like 8 [Benincasa hispida])

HSP 1 Score: 411.0 bits (1055), Expect = 7.3e-111
Identity = 191/244 (78.28%), Postives = 216/244 (88.52%), Query Frame = 0

Query: 3   GGNISSQGGKTNPTSLFLSLFLGKANELLAILKTTFPELGFEKKDCIETSWIESALIMGN 62
           GGN + Q GKTNPT+LF SL+LGK +EL++IL  TFPELG  KKDCIETSW+ESALIMG 
Sbjct: 292 GGNFTRQEGKTNPTALFFSLYLGKVDELVSILNKTFPELGLAKKDCIETSWVESALIMGT 351

Query: 63  RFQRGKTLEELLSRTPLTNGSTKIKSDYIKEPIPEAAIQGIWERLKSQDIEMPQLLFVPY 122
             Q GK+LE+LLS+  LTNG+TKIKSDYIK+PIP AA++GIW+RLK +DIEMPQLLF PY
Sbjct: 352 GLQPGKSLEDLLSKASLTNGNTKIKSDYIKQPIPLAAVEGIWQRLKLEDIEMPQLLFAPY 411

Query: 123 GGRMSQISESETPFSHRAGYLYKIGYVLEWEEGSVDAENRHLSWIRELYAYMAPFVSKSP 182
           GGRMSQISESE+PFSHRAGYLYKIGYV  WE+   D E RHLSWIRELY+YM PFVSKSP
Sbjct: 412 GGRMSQISESESPFSHRAGYLYKIGYVTYWEQQDNDVEKRHLSWIRELYSYMTPFVSKSP 471

Query: 183 RAAYVNYRDLDIGSNNKYGKTSYKRASVWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSI 242
           RAAY+NYRDLDIGSNNKYGKTSYK+AS+WGLKYFGNNFNRLV VKT+VDP++FFRHEQSI
Sbjct: 472 RAAYINYRDLDIGSNNKYGKTSYKQASIWGLKYFGNNFNRLVDVKTRVDPHNFFRHEQSI 531

Query: 243 PTLS 247
           PT S
Sbjct: 532 PTFS 535

BLAST of Sgr012799 vs. NCBI nr
Match: XP_022142587.1 (tetrahydrocannabinolic acid synthase-like [Momordica charantia])

HSP 1 Score: 401.0 bits (1029), Expect = 7.6e-108
Identity = 192/245 (78.37%), Postives = 210/245 (85.71%), Query Frame = 0

Query: 2   IGGNISSQ-GGKTNPTSLFLSLFLGKANELLAILKTTFPELGFEKKDCIETSWIESALIM 61
           +GG I+SQ GGKTNP + F SLFLGKA+ELL+IL T FPEL   KKDCIETSWIES ++M
Sbjct: 295 LGGKITSQEGGKTNPVATFFSLFLGKADELLSILNTKFPELDLAKKDCIETSWIESTVLM 354

Query: 62  GNRFQRGKTLEELLSRTPLTNGSTKIKSDYIKEPIPEAAIQGIWERLKSQDIEMPQLLFV 121
           G  F    TLE LLSRTPLTN S+KIKSDY+KEP+ EA +QGIW+RL SQDIE   ++FV
Sbjct: 355 GVGFPTELTLEALLSRTPLTNTSSKIKSDYVKEPMSEAVVQGIWKRLDSQDIEGGNIIFV 414

Query: 122 PYGGRMSQISESETPFSHRAGYLYKIGYVLEWEEGSVDAENRHLSWIRELYAYMAPFVSK 181
           PYGGRMSQISESETPFSHRAGYLYKIGY+  WEE  +DAE RHL WIRELY YM PFVSK
Sbjct: 415 PYGGRMSQISESETPFSHRAGYLYKIGYIAGWEEQGIDAEKRHLRWIRELYDYMTPFVSK 474

Query: 182 SPRAAYVNYRDLDIGSNNKYGKTSYKRASVWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQ 241
           SPRAAY NYRDLDIGSNNK+GKTSYK+ASVWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQ
Sbjct: 475 SPRAAYANYRDLDIGSNNKFGKTSYKQASVWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQ 534

Query: 242 SIPTL 246
           SIP L
Sbjct: 535 SIPAL 539

BLAST of Sgr012799 vs. NCBI nr
Match: XP_022152516.1 (berberine bridge enzyme-like 18 [Momordica charantia])

HSP 1 Score: 400.6 bits (1028), Expect = 9.9e-108
Identity = 190/248 (76.61%), Postives = 217/248 (87.50%), Query Frame = 0

Query: 1   MIGGNISSQGG-KTNPTSLFLSLFLGKANELLAILKTTFPELGFEKKDCIETSWIESALI 60
           ++GGN ++QGG KTNPT+ F SLFLGKA+E  AIL TTFPELG  K++C+E+SWIESAL+
Sbjct: 295 LMGGNFTAQGGDKTNPTASFFSLFLGKADEFFAILNTTFPELGLAKENCLESSWIESALL 354

Query: 61  MGNRFQRGKTLEELLSRTPLTNGSTKIKSDYIKEPIPEAAIQGIWERLKSQDIEMPQLLF 120
               F+ G +LE LL+RTPLTNGS KIKSDY+KEPI EA I+GIWERLKS+D+   QLLF
Sbjct: 355 SAG-FKIGDSLEALLNRTPLTNGSIKIKSDYVKEPISEATIEGIWERLKSRDVGARQLLF 414

Query: 121 VPYGGRMSQISESETPFSHRAGYLYKIGYVLEWEEGSVDAENRHLSWIRELYAYMAPFVS 180
           VPYGGRMSQISESE PFSHRAG LYKIGY++ W++ +VD E RHL WIRELY+YM PFVS
Sbjct: 415 VPYGGRMSQISESEIPFSHRAGNLYKIGYIVAWQKQNVDVEKRHLKWIRELYSYMTPFVS 474

Query: 181 KSPRAAYVNYRDLDIGSNNKYGKTSYKRASVWGLKYFGNNFNRLVHVKTKVDPYDFFRHE 240
           KSPR+AYVNYRDLDIGSNNKYGKTS+KRASVWG KYFGNNFNRLVHVKTKVDPYDFFRHE
Sbjct: 475 KSPRSAYVNYRDLDIGSNNKYGKTSFKRASVWGWKYFGNNFNRLVHVKTKVDPYDFFRHE 534

Query: 241 QSIPTLSP 248
           QSIPT+SP
Sbjct: 535 QSIPTISP 541

BLAST of Sgr012799 vs. NCBI nr
Match: XP_022142425.1 (berberine bridge enzyme-like 18 [Momordica charantia])

HSP 1 Score: 398.7 bits (1023), Expect = 3.8e-107
Identity = 192/245 (78.37%), Postives = 209/245 (85.31%), Query Frame = 0

Query: 2   IGGNISSQ-GGKTNPTSLFLSLFLGKANELLAILKTTFPELGFEKKDCIETSWIESALIM 61
           +GGN++SQ GGKTNP + F SLFLG+A+ELLAIL T FPEL   KKDCIETSWIES ++M
Sbjct: 296 LGGNVTSQGGGKTNPVATFFSLFLGQADELLAILNTKFPELDLAKKDCIETSWIESTVLM 355

Query: 62  GNRFQRGKTLEELLSRTPLTNGSTKIKSDYIKEPIPEAAIQGIWERLKSQDIEMPQLLFV 121
           G  FQ   TLE LLSRTPLTN STKIKSDY+KEPI EA IQGI ERL +QDIE   L+FV
Sbjct: 356 GIGFQTKVTLEALLSRTPLTNISTKIKSDYVKEPISEATIQGIGERLNAQDIESGNLIFV 415

Query: 122 PYGGRMSQISESETPFSHRAGYLYKIGYVLEWEEGSVDAENRHLSWIRELYAYMAPFVSK 181
           PYGGRMSQI ESETPFSHRAGYLYKIGY+  W + S+D E RHLSWIRELY+YMAPFVSK
Sbjct: 416 PYGGRMSQIFESETPFSHRAGYLYKIGYITSWLDQSIDTEKRHLSWIRELYSYMAPFVSK 475

Query: 182 SPRAAYVNYRDLDIGSNNKYGKTSYKRASVWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQ 241
           SPRA Y NYRDLDIGSN +YGKTSYK+AS WG KYFGNNFNRLVHVKTKVDPYDFFRHEQ
Sbjct: 476 SPRAVYANYRDLDIGSNKRYGKTSYKQASTWGFKYFGNNFNRLVHVKTKVDPYDFFRHEQ 535

Query: 242 SIPTL 246
           SIPTL
Sbjct: 536 SIPTL 540

BLAST of Sgr012799 vs. NCBI nr
Match: XP_022994835.1 (berberine bridge enzyme-like 18 [Cucurbita maxima])

HSP 1 Score: 398.3 bits (1022), Expect = 4.9e-107
Identity = 184/247 (74.49%), Postives = 211/247 (85.43%), Query Frame = 0

Query: 1   MIGGNISSQGGKTNPTSLFLSLFLGKANELLAILKTTFPELGFEKKDCIETSWIESALIM 60
           + GGN + QGGK NP +LF SL+LGKA+ELL IL TTFPELG  KKDC+ETSW+E+ALIM
Sbjct: 297 LAGGNFTRQGGKINPLALFFSLYLGKADELLTILNTTFPELGLSKKDCMETSWVETALIM 356

Query: 61  GNRFQRGKTLEELLSRTPLTNGSTKIKSDYIKEPIPEAAIQGIWERLKSQDIEMPQLLFV 120
           G   Q G  LE+LLS+ PL NGSTKIKSDYIKEPIP AAI+GIWERL+ +DIEMPQ +FV
Sbjct: 357 GTGLQLGSKLEDLLSKAPLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFV 416

Query: 121 PYGGRMSQISESETPFSHRAGYLYKIGYVLEWEEGSVDAENRHLSWIRELYAYMAPFVSK 180
           PYGG+M QI E ETPFSHR  YLYKIGY++ W E  ++AE RH+ WIRELY+YM PFVSK
Sbjct: 417 PYGGKMGQIPEFETPFSHRGEYLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSK 476

Query: 181 SPRAAYVNYRDLDIGSNNKYGKTSYKRASVWGLKYFG-NNFNRLVHVKTKVDPYDFFRHE 240
           SPRAAY+NYRDLDIGSNNKYG+TSY++AS+WG KYFG  NF+RLVHVKTKVDPYDFFRHE
Sbjct: 477 SPRAAYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHE 536

Query: 241 QSIPTLS 247
           QSIPTLS
Sbjct: 537 QSIPTLS 543

BLAST of Sgr012799 vs. ExPASy Swiss-Prot
Match: Q9FKU8 (Berberine bridge enzyme-like 26 OS=Arabidopsis thaliana OX=3702 GN=At5g44400 PE=2 SV=1)

HSP 1 Score: 246.1 bits (627), Expect = 4.1e-64
Identity = 118/241 (48.96%), Postives = 161/241 (66.80%), Query Frame = 0

Query: 5   NISSQGGKTNPTSLFLSLFLGKANELLAILKTTFPELGFEKKDCIETSWIESALIMGNRF 64
           N++  GG    T+ + +LFLG    L+ ++K +FPELG   KDCIE SW+ES   +    
Sbjct: 299 NVAGTGGNKTVTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYISGFP 358

Query: 65  QRGKTLEELLSRTPLTNGSTKIKSDYIKEPIPEAAIQGIWERLKSQDIEMPQLLFVPYGG 124
               T   L  ++P    S K KSD++K PIPE+ +QGI+++L  +DI  P +++ PYGG
Sbjct: 359 THTPTNVLLQGKSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDI--PLMIWNPYGG 418

Query: 125 RMSQISESETPFSHRAGYLYKIGYVLEWEEGSVDAENRHLSWIRELYAYMAPFVSKSPRA 184
            M++I ES+ PF HR G L+K+ YV  W + S    +RH++WIR+LY+YM P+VS +PR 
Sbjct: 419 MMAKIPESQIPFPHRKGVLFKVQYVTSWLD-SDKRPSRHINWIRDLYSYMTPYVSSNPRE 478

Query: 185 AYVNYRDLDIGSNNKYGKTSYKRASVWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPT 244
           AYVNYRDLD+G N K  KT  K+A VWG  YF NNFNRL+ +K KVDP +FFRHEQSIP 
Sbjct: 479 AYVNYRDLDLGRNTKDVKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQSIPP 536

Query: 245 L 246
           +
Sbjct: 539 M 536

BLAST of Sgr012799 vs. ExPASy Swiss-Prot
Match: Q9FI21 (Berberine bridge enzyme-like 28 OS=Arabidopsis thaliana OX=3702 GN=At5g44440 PE=1 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 6.1e-60
Identity = 112/233 (48.07%), Postives = 159/233 (68.24%), Query Frame = 0

Query: 17  SLFLSLFLGKANELLAILKTTFPELGFEKKDCIETSWIESALIMGNRFQRGKTLEELLSR 76
           +LF  L++G  N LLA+++  FPELG EK+ C E SWIES L   + F +G++L  L +R
Sbjct: 300 ALFTGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWFAD-FPKGESLGVLTNR 359

Query: 77  TPLTNGSTKIKSDYIKEPIPEAAIQGIWERLKSQDIEMPQLLFVPYGGRMSQISESETPF 136
              T+ S K K D+++EPIPEAAIQ IW RL++ +  + +++  P+GG+MS+++E ETPF
Sbjct: 360 -ERTSLSFKGKDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPFGGKMSEMAEYETPF 419

Query: 137 SHRAGYLYKIGYVLEW---EEGSVDAENRHLSWIRELYAYMAPFVSKSPRAAYVNYRDLD 196
            HR G LY+I YV  W   E+ +    +++L W+  +Y +M P+VSKSPR AYVN++D+D
Sbjct: 420 PHRGGNLYEIQYVAYWREEEDKNKTETDKYLKWVDSVYEFMTPYVSKSPRGAYVNFKDMD 479

Query: 197 IGSNNKYGKTSYKRASVWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPTLS 247
           +G      KT Y+    WG+KYF NNF RLV VKT+VDP DFF  EQSIP ++
Sbjct: 480 LGMYLGKKKTKYEEGKSWGVKYFKNNFERLVRVKTRVDPTDFFCDEQSIPLVN 530

BLAST of Sgr012799 vs. ExPASy Swiss-Prot
Match: O64743 (Berberine bridge enzyme-like 15 OS=Arabidopsis thaliana OX=3702 GN=MEE23 PE=1 SV=1)

HSP 1 Score: 229.9 bits (585), Expect = 3.0e-59
Identity = 117/244 (47.95%), Postives = 157/244 (64.34%), Query Frame = 0

Query: 6   ISSQGGKTNPTSLFLSL-----FLGKANELLAILKTTFPELGFEKKDCIETSWIESALIM 65
           IS     T P +  +S+     FLG +N LL +++ +FPELG  KKDC E SWI+S + +
Sbjct: 295 ISPASKTTKPGNRTISMSYQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYI 354

Query: 66  GNRFQRGKTLEELLSRTPLTNGSTKIKSDYIKEPIPEAAIQGIWERLKSQDIEMPQLLFV 125
              F      E LL+   L     K KSD++KEPIP   ++G+WER   +D   P  ++ 
Sbjct: 355 AG-FPNSAAPEALLAGKSLFKNHFKAKSDFVKEPIPVEGLEGLWERFLEED--SPLTIWN 414

Query: 126 PYGGRMSQISESETPFSHRAGYLYKIGYVLEWEEGSVDAENRHLSWIRELYAYMAPFVSK 185
           PYGG MS+ISESE PF HR G L+KI ++  W++G V +E RH+ WIRE+Y+YM  +VSK
Sbjct: 415 PYGGMMSRISESEIPFPHRNGTLFKIQWLSTWQDGKV-SEERHMKWIREMYSYMEQYVSK 474

Query: 186 SPRAAYVNYRDLDIGSNNKYGKTSYKRASVWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQ 245
           +PR AYVNYRDLD+G+N   G+T    A  WG KY+  NF RLV +K + DP +FFRHEQ
Sbjct: 475 NPRQAYVNYRDLDLGTNE--GETD---AREWGAKYYKGNFERLVKIKGEFDPDNFFRHEQ 529

BLAST of Sgr012799 vs. ExPASy Swiss-Prot
Match: Q93ZA3 (Berberine bridge enzyme-like 13 OS=Arabidopsis thaliana OX=3702 GN=At1g30760 PE=1 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 6.8e-59
Identity = 110/222 (49.55%), Postives = 149/222 (67.12%), Query Frame = 0

Query: 23  FLGKANELLAILKTTFPELGFEKKDCIETSWIESALIMGNRFQRGKTLEELLSRTPLTNG 82
           FLG AN LL +++ +FP+LG  KKDC+ETSWI+S + +   F      E LL    L   
Sbjct: 319 FLGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMYIAG-FPSTAPSEALLDGKSLFKN 378

Query: 83  STKIKSDYIKEPIPEAAIQGIWERLKSQDIEMPQLLFVPYGGRMSQISESETPFSHRAGY 142
             K KSDY++EPIP   ++G+WE+L  +D   P  ++ PYGG M++I E+ETPF HR+G 
Sbjct: 379 YFKAKSDYVEEPIPVEGLEGLWEKLLEED--SPLTIWNPYGGMMAKIPETETPFPHRSGT 438

Query: 143 LYKIGYVLEWEEGSVDAENRHLSWIRELYAYMAPFVSKSPRAAYVNYRDLDIGSNNKYGK 202
           L+KI ++  W++G   +E +H+ W+RE+Y+YM  +VSKSPR+AYVNYRDLD+G N K   
Sbjct: 439 LFKIQWLTLWQDGKT-SEAKHMGWMREMYSYMEQYVSKSPRSAYVNYRDLDLGMNGKGSD 498

Query: 203 TSYKRASVWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPT 245
                A  WG +YF  NF RLV +K K DP +FFRHEQSIPT
Sbjct: 499 -----AREWGNRYFKGNFERLVEIKAKFDPENFFRHEQSIPT 531

BLAST of Sgr012799 vs. ExPASy Swiss-Prot
Match: A6P6V9 (Cannabidiolic acid synthase OS=Cannabis sativa OX=3483 GN=CBDAS PE=1 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 8.8e-59
Identity = 117/247 (47.37%), Postives = 158/247 (63.97%), Query Frame = 0

Query: 5   NISSQGGKTNPTSL---FLSLFLGKANELLAILKTTFPELGFEKKDCIETSWIESALIMG 64
           NI+   GK N T++   F S+FLG  + L+ ++  +FPELG +K DC + SWI++ +   
Sbjct: 296 NITDNQGK-NKTAIHTYFSSVFLGGVDSLVDLMNKSFPELGIKKTDCRQLSWIDTIIFYS 355

Query: 65  ---NRFQRGKTLEELLSRTPLTNGSTKIKSDYIKEPIPEAAIQGIWERLKSQDIEMPQLL 124
              N        E LL R+   NG+ KIK DY+K+PIPE+    I E+L  +DI      
Sbjct: 356 GVVNYDTDNFNKEILLDRSAGQNGAFKIKLDYVKKPIPESVFVQILEKLYEEDIGAGMYA 415

Query: 125 FVPYGGRMSQISESETPFSHRAGYLYKIGYVLEWEEGSVDAENRHLSWIRELYAYMAPFV 184
             PYGG M +ISES  PF HRAG LY++ Y+  WE+   +   +HL+WIR +Y +M P+V
Sbjct: 416 LYPYGGIMDEISESAIPFPHRAGILYELWYICSWEK--QEDNEKHLNWIRNIYNFMTPYV 475

Query: 185 SKSPRAAYVNYRDLDIGSNNKYGKTSYKRASVWGLKYFGNNFNRLVHVKTKVDPYDFFRH 244
           SK+PR AY+NYRDLDIG N+     +Y +A +WG KYFG NF+RLV VKT VDP +FFR+
Sbjct: 476 SKNPRLAYLNYRDLDIGINDPKNPNNYTQARIWGEKYFGKNFDRLVKVKTLVDPNNFFRN 535

Query: 245 EQSIPTL 246
           EQSIP L
Sbjct: 536 EQSIPPL 539

BLAST of Sgr012799 vs. ExPASy TrEMBL
Match: A0A6J1CN68 (tetrahydrocannabinolic acid synthase-like OS=Momordica charantia OX=3673 GN=LOC111012666 PE=3 SV=1)

HSP 1 Score: 401.0 bits (1029), Expect = 3.7e-108
Identity = 192/245 (78.37%), Postives = 210/245 (85.71%), Query Frame = 0

Query: 2   IGGNISSQ-GGKTNPTSLFLSLFLGKANELLAILKTTFPELGFEKKDCIETSWIESALIM 61
           +GG I+SQ GGKTNP + F SLFLGKA+ELL+IL T FPEL   KKDCIETSWIES ++M
Sbjct: 295 LGGKITSQEGGKTNPVATFFSLFLGKADELLSILNTKFPELDLAKKDCIETSWIESTVLM 354

Query: 62  GNRFQRGKTLEELLSRTPLTNGSTKIKSDYIKEPIPEAAIQGIWERLKSQDIEMPQLLFV 121
           G  F    TLE LLSRTPLTN S+KIKSDY+KEP+ EA +QGIW+RL SQDIE   ++FV
Sbjct: 355 GVGFPTELTLEALLSRTPLTNTSSKIKSDYVKEPMSEAVVQGIWKRLDSQDIEGGNIIFV 414

Query: 122 PYGGRMSQISESETPFSHRAGYLYKIGYVLEWEEGSVDAENRHLSWIRELYAYMAPFVSK 181
           PYGGRMSQISESETPFSHRAGYLYKIGY+  WEE  +DAE RHL WIRELY YM PFVSK
Sbjct: 415 PYGGRMSQISESETPFSHRAGYLYKIGYIAGWEEQGIDAEKRHLRWIRELYDYMTPFVSK 474

Query: 182 SPRAAYVNYRDLDIGSNNKYGKTSYKRASVWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQ 241
           SPRAAY NYRDLDIGSNNK+GKTSYK+ASVWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQ
Sbjct: 475 SPRAAYANYRDLDIGSNNKFGKTSYKQASVWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQ 534

Query: 242 SIPTL 246
           SIP L
Sbjct: 535 SIPAL 539

BLAST of Sgr012799 vs. ExPASy TrEMBL
Match: A0A6J1DHZ2 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020223 PE=3 SV=1)

HSP 1 Score: 400.6 bits (1028), Expect = 4.8e-108
Identity = 190/248 (76.61%), Postives = 217/248 (87.50%), Query Frame = 0

Query: 1   MIGGNISSQGG-KTNPTSLFLSLFLGKANELLAILKTTFPELGFEKKDCIETSWIESALI 60
           ++GGN ++QGG KTNPT+ F SLFLGKA+E  AIL TTFPELG  K++C+E+SWIESAL+
Sbjct: 295 LMGGNFTAQGGDKTNPTASFFSLFLGKADEFFAILNTTFPELGLAKENCLESSWIESALL 354

Query: 61  MGNRFQRGKTLEELLSRTPLTNGSTKIKSDYIKEPIPEAAIQGIWERLKSQDIEMPQLLF 120
               F+ G +LE LL+RTPLTNGS KIKSDY+KEPI EA I+GIWERLKS+D+   QLLF
Sbjct: 355 SAG-FKIGDSLEALLNRTPLTNGSIKIKSDYVKEPISEATIEGIWERLKSRDVGARQLLF 414

Query: 121 VPYGGRMSQISESETPFSHRAGYLYKIGYVLEWEEGSVDAENRHLSWIRELYAYMAPFVS 180
           VPYGGRMSQISESE PFSHRAG LYKIGY++ W++ +VD E RHL WIRELY+YM PFVS
Sbjct: 415 VPYGGRMSQISESEIPFSHRAGNLYKIGYIVAWQKQNVDVEKRHLKWIRELYSYMTPFVS 474

Query: 181 KSPRAAYVNYRDLDIGSNNKYGKTSYKRASVWGLKYFGNNFNRLVHVKTKVDPYDFFRHE 240
           KSPR+AYVNYRDLDIGSNNKYGKTS+KRASVWG KYFGNNFNRLVHVKTKVDPYDFFRHE
Sbjct: 475 KSPRSAYVNYRDLDIGSNNKYGKTSFKRASVWGWKYFGNNFNRLVHVKTKVDPYDFFRHE 534

Query: 241 QSIPTLSP 248
           QSIPT+SP
Sbjct: 535 QSIPTISP 541

BLAST of Sgr012799 vs. ExPASy TrEMBL
Match: A0A6J1CKW8 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111012562 PE=3 SV=1)

HSP 1 Score: 398.7 bits (1023), Expect = 1.8e-107
Identity = 192/245 (78.37%), Postives = 209/245 (85.31%), Query Frame = 0

Query: 2   IGGNISSQ-GGKTNPTSLFLSLFLGKANELLAILKTTFPELGFEKKDCIETSWIESALIM 61
           +GGN++SQ GGKTNP + F SLFLG+A+ELLAIL T FPEL   KKDCIETSWIES ++M
Sbjct: 296 LGGNVTSQGGGKTNPVATFFSLFLGQADELLAILNTKFPELDLAKKDCIETSWIESTVLM 355

Query: 62  GNRFQRGKTLEELLSRTPLTNGSTKIKSDYIKEPIPEAAIQGIWERLKSQDIEMPQLLFV 121
           G  FQ   TLE LLSRTPLTN STKIKSDY+KEPI EA IQGI ERL +QDIE   L+FV
Sbjct: 356 GIGFQTKVTLEALLSRTPLTNISTKIKSDYVKEPISEATIQGIGERLNAQDIESGNLIFV 415

Query: 122 PYGGRMSQISESETPFSHRAGYLYKIGYVLEWEEGSVDAENRHLSWIRELYAYMAPFVSK 181
           PYGGRMSQI ESETPFSHRAGYLYKIGY+  W + S+D E RHLSWIRELY+YMAPFVSK
Sbjct: 416 PYGGRMSQIFESETPFSHRAGYLYKIGYITSWLDQSIDTEKRHLSWIRELYSYMAPFVSK 475

Query: 182 SPRAAYVNYRDLDIGSNNKYGKTSYKRASVWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQ 241
           SPRA Y NYRDLDIGSN +YGKTSYK+AS WG KYFGNNFNRLVHVKTKVDPYDFFRHEQ
Sbjct: 476 SPRAVYANYRDLDIGSNKRYGKTSYKQASTWGFKYFGNNFNRLVHVKTKVDPYDFFRHEQ 535

Query: 242 SIPTL 246
           SIPTL
Sbjct: 536 SIPTL 540

BLAST of Sgr012799 vs. ExPASy TrEMBL
Match: A0A6J1K2F5 (berberine bridge enzyme-like 18 OS=Cucurbita maxima OX=3661 GN=LOC111490442 PE=3 SV=1)

HSP 1 Score: 398.3 bits (1022), Expect = 2.4e-107
Identity = 184/247 (74.49%), Postives = 211/247 (85.43%), Query Frame = 0

Query: 1   MIGGNISSQGGKTNPTSLFLSLFLGKANELLAILKTTFPELGFEKKDCIETSWIESALIM 60
           + GGN + QGGK NP +LF SL+LGKA+ELL IL TTFPELG  KKDC+ETSW+E+ALIM
Sbjct: 297 LAGGNFTRQGGKINPLALFFSLYLGKADELLTILNTTFPELGLSKKDCMETSWVETALIM 356

Query: 61  GNRFQRGKTLEELLSRTPLTNGSTKIKSDYIKEPIPEAAIQGIWERLKSQDIEMPQLLFV 120
           G   Q G  LE+LLS+ PL NGSTKIKSDYIKEPIP AAI+GIWERL+ +DIEMPQ +FV
Sbjct: 357 GTGLQLGSKLEDLLSKAPLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFV 416

Query: 121 PYGGRMSQISESETPFSHRAGYLYKIGYVLEWEEGSVDAENRHLSWIRELYAYMAPFVSK 180
           PYGG+M QI E ETPFSHR  YLYKIGY++ W E  ++AE RH+ WIRELY+YM PFVSK
Sbjct: 417 PYGGKMGQIPEFETPFSHRGEYLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSK 476

Query: 181 SPRAAYVNYRDLDIGSNNKYGKTSYKRASVWGLKYFG-NNFNRLVHVKTKVDPYDFFRHE 240
           SPRAAY+NYRDLDIGSNNKYG+TSY++AS+WG KYFG  NF+RLVHVKTKVDPYDFFRHE
Sbjct: 477 SPRAAYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHE 536

Query: 241 QSIPTLS 247
           QSIPTLS
Sbjct: 537 QSIPTLS 543

BLAST of Sgr012799 vs. ExPASy TrEMBL
Match: A0A6J1GRE1 (berberine bridge enzyme-like 18 OS=Cucurbita moschata OX=3662 GN=LOC111456783 PE=3 SV=1)

HSP 1 Score: 393.7 bits (1010), Expect = 5.9e-106
Identity = 183/247 (74.09%), Postives = 209/247 (84.62%), Query Frame = 0

Query: 1   MIGGNISSQGGKTNPTSLFLSLFLGKANELLAILKTTFPELGFEKKDCIETSWIESALIM 60
           + GGN + QGGK NP +LF SL+LGKANELL IL TTFPELG  KKDC+ETSW+E+ALIM
Sbjct: 297 LAGGNFTRQGGKINPLALFFSLYLGKANELLTILNTTFPELGLSKKDCMETSWVETALIM 356

Query: 61  GNRFQRGKTLEELLSRTPLTNGSTKIKSDYIKEPIPEAAIQGIWERLKSQDIEMPQLLFV 120
           G   Q G  LE+LLS+  L NGSTKIKSDYIKEPIP AAI+GIWERL+ +DIEMPQ +FV
Sbjct: 357 GTGLQLGSKLEDLLSKATLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFV 416

Query: 121 PYGGRMSQISESETPFSHRAGYLYKIGYVLEWEEGSVDAENRHLSWIRELYAYMAPFVSK 180
           PYGG+M QI E ETPFSHR   LYKIGY++ W E  ++AE RH+ WIRELY+YM PFVSK
Sbjct: 417 PYGGKMGQIPEFETPFSHRGENLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSK 476

Query: 181 SPRAAYVNYRDLDIGSNNKYGKTSYKRASVWGLKYFG-NNFNRLVHVKTKVDPYDFFRHE 240
           SPRAAY+NYRDLDIGSNNKYG+TSY++AS+WG KYFG  NF+RLVHVKTKVDPYDFFRHE
Sbjct: 477 SPRAAYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHE 536

Query: 241 QSIPTLS 247
           QSIPTLS
Sbjct: 537 QSIPTLS 543

BLAST of Sgr012799 vs. TAIR 10
Match: AT5G44400.1 (FAD-binding Berberine family protein )

HSP 1 Score: 246.1 bits (627), Expect = 2.9e-65
Identity = 118/241 (48.96%), Postives = 161/241 (66.80%), Query Frame = 0

Query: 5   NISSQGGKTNPTSLFLSLFLGKANELLAILKTTFPELGFEKKDCIETSWIESALIMGNRF 64
           N++  GG    T+ + +LFLG    L+ ++K +FPELG   KDCIE SW+ES   +    
Sbjct: 299 NVAGTGGNKTVTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYISGFP 358

Query: 65  QRGKTLEELLSRTPLTNGSTKIKSDYIKEPIPEAAIQGIWERLKSQDIEMPQLLFVPYGG 124
               T   L  ++P    S K KSD++K PIPE+ +QGI+++L  +DI  P +++ PYGG
Sbjct: 359 THTPTNVLLQGKSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDI--PLMIWNPYGG 418

Query: 125 RMSQISESETPFSHRAGYLYKIGYVLEWEEGSVDAENRHLSWIRELYAYMAPFVSKSPRA 184
            M++I ES+ PF HR G L+K+ YV  W + S    +RH++WIR+LY+YM P+VS +PR 
Sbjct: 419 MMAKIPESQIPFPHRKGVLFKVQYVTSWLD-SDKRPSRHINWIRDLYSYMTPYVSSNPRE 478

Query: 185 AYVNYRDLDIGSNNKYGKTSYKRASVWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPT 244
           AYVNYRDLD+G N K  KT  K+A VWG  YF NNFNRL+ +K KVDP +FFRHEQSIP 
Sbjct: 479 AYVNYRDLDLGRNTKDVKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQSIPP 536

Query: 245 L 246
           +
Sbjct: 539 M 536

BLAST of Sgr012799 vs. TAIR 10
Match: AT5G44440.1 (FAD-binding Berberine family protein )

HSP 1 Score: 232.3 bits (591), Expect = 4.3e-61
Identity = 112/233 (48.07%), Postives = 159/233 (68.24%), Query Frame = 0

Query: 17  SLFLSLFLGKANELLAILKTTFPELGFEKKDCIETSWIESALIMGNRFQRGKTLEELLSR 76
           +LF  L++G  N LLA+++  FPELG EK+ C E SWIES L   + F +G++L  L +R
Sbjct: 300 ALFTGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWFAD-FPKGESLGVLTNR 359

Query: 77  TPLTNGSTKIKSDYIKEPIPEAAIQGIWERLKSQDIEMPQLLFVPYGGRMSQISESETPF 136
              T+ S K K D+++EPIPEAAIQ IW RL++ +  + +++  P+GG+MS+++E ETPF
Sbjct: 360 -ERTSLSFKGKDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPFGGKMSEMAEYETPF 419

Query: 137 SHRAGYLYKIGYVLEW---EEGSVDAENRHLSWIRELYAYMAPFVSKSPRAAYVNYRDLD 196
            HR G LY+I YV  W   E+ +    +++L W+  +Y +M P+VSKSPR AYVN++D+D
Sbjct: 420 PHRGGNLYEIQYVAYWREEEDKNKTETDKYLKWVDSVYEFMTPYVSKSPRGAYVNFKDMD 479

Query: 197 IGSNNKYGKTSYKRASVWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPTLS 247
           +G      KT Y+    WG+KYF NNF RLV VKT+VDP DFF  EQSIP ++
Sbjct: 480 LGMYLGKKKTKYEEGKSWGVKYFKNNFERLVRVKTRVDPTDFFCDEQSIPLVN 530

BLAST of Sgr012799 vs. TAIR 10
Match: AT2G34790.1 (FAD-binding Berberine family protein )

HSP 1 Score: 229.9 bits (585), Expect = 2.2e-60
Identity = 117/244 (47.95%), Postives = 157/244 (64.34%), Query Frame = 0

Query: 6   ISSQGGKTNPTSLFLSL-----FLGKANELLAILKTTFPELGFEKKDCIETSWIESALIM 65
           IS     T P +  +S+     FLG +N LL +++ +FPELG  KKDC E SWI+S + +
Sbjct: 295 ISPASKTTKPGNRTISMSYQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYI 354

Query: 66  GNRFQRGKTLEELLSRTPLTNGSTKIKSDYIKEPIPEAAIQGIWERLKSQDIEMPQLLFV 125
              F      E LL+   L     K KSD++KEPIP   ++G+WER   +D   P  ++ 
Sbjct: 355 AG-FPNSAAPEALLAGKSLFKNHFKAKSDFVKEPIPVEGLEGLWERFLEED--SPLTIWN 414

Query: 126 PYGGRMSQISESETPFSHRAGYLYKIGYVLEWEEGSVDAENRHLSWIRELYAYMAPFVSK 185
           PYGG MS+ISESE PF HR G L+KI ++  W++G V +E RH+ WIRE+Y+YM  +VSK
Sbjct: 415 PYGGMMSRISESEIPFPHRNGTLFKIQWLSTWQDGKV-SEERHMKWIREMYSYMEQYVSK 474

Query: 186 SPRAAYVNYRDLDIGSNNKYGKTSYKRASVWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQ 245
           +PR AYVNYRDLD+G+N   G+T    A  WG KY+  NF RLV +K + DP +FFRHEQ
Sbjct: 475 NPRQAYVNYRDLDLGTNE--GETD---AREWGAKYYKGNFERLVKIKGEFDPDNFFRHEQ 529

BLAST of Sgr012799 vs. TAIR 10
Match: AT1G30760.1 (FAD-binding Berberine family protein )

HSP 1 Score: 228.8 bits (582), Expect = 4.8e-60
Identity = 110/222 (49.55%), Postives = 149/222 (67.12%), Query Frame = 0

Query: 23  FLGKANELLAILKTTFPELGFEKKDCIETSWIESALIMGNRFQRGKTLEELLSRTPLTNG 82
           FLG AN LL +++ +FP+LG  KKDC+ETSWI+S + +   F      E LL    L   
Sbjct: 319 FLGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMYIAG-FPSTAPSEALLDGKSLFKN 378

Query: 83  STKIKSDYIKEPIPEAAIQGIWERLKSQDIEMPQLLFVPYGGRMSQISESETPFSHRAGY 142
             K KSDY++EPIP   ++G+WE+L  +D   P  ++ PYGG M++I E+ETPF HR+G 
Sbjct: 379 YFKAKSDYVEEPIPVEGLEGLWEKLLEED--SPLTIWNPYGGMMAKIPETETPFPHRSGT 438

Query: 143 LYKIGYVLEWEEGSVDAENRHLSWIRELYAYMAPFVSKSPRAAYVNYRDLDIGSNNKYGK 202
           L+KI ++  W++G   +E +H+ W+RE+Y+YM  +VSKSPR+AYVNYRDLD+G N K   
Sbjct: 439 LFKIQWLTLWQDGKT-SEAKHMGWMREMYSYMEQYVSKSPRSAYVNYRDLDLGMNGKGSD 498

Query: 203 TSYKRASVWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPT 245
                A  WG +YF  NF RLV +K K DP +FFRHEQSIPT
Sbjct: 499 -----AREWGNRYFKGNFERLVEIKAKFDPENFFRHEQSIPT 531

BLAST of Sgr012799 vs. TAIR 10
Match: AT5G44380.1 (FAD-binding Berberine family protein )

HSP 1 Score: 227.3 bits (578), Expect = 1.4e-59
Identity = 115/236 (48.73%), Postives = 149/236 (63.14%), Query Frame = 0

Query: 11  GKTNPTSLFLSLFLGKANELLAILKTTFPELGFEKKDCIETSWIESALIMGNRFQRGKTL 70
           G    T  +L  FLG+   LL +++  FPELG  +KDC E SWIE+AL  G  F  G  +
Sbjct: 308 GNKTVTMTYLGQFLGEKGTLLKVMEKAFPELGLTQKDCTEMSWIEAALFHGG-FPTGSPI 367

Query: 71  EELLS-RTPLTNGSTKIKSDYIKEPIPEAAIQGIWERLKSQDIEMPQLLFVPYGGRMSQI 130
           E LL  ++PL     K  SD++KEPIP   ++GI++RL   +     L + PYGG MS+I
Sbjct: 368 EILLQLKSPLGKDYFKATSDFVKEPIPVIGLKGIFKRLIEGNTTF--LNWTPYGGMMSKI 427

Query: 131 SESETPFSHRAGYLYKIGYVLEWEEGSVDAENRHLSWIRELYAYMAPFVSKSPRAAYVNY 190
            ES  PF HR G L+KI Y   W E      +R ++WI+E+Y YMAP+VS +PR AYVNY
Sbjct: 428 PESAIPFPHRNGTLFKILYYANWLEND-KTSSRKINWIKEIYNYMAPYVSSNPRQAYVNY 487

Query: 191 RDLDIGSNNKYGKTSYKRASVWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPTL 246
           RDLD G N    K ++  A +WG KYF  NF+RLV +KTKVDP +FFRHEQSIP +
Sbjct: 488 RDLDFGQNKNNAKVNFIEAKIWGPKYFKGNFDRLVKIKTKVDPENFFRHEQSIPPM 539