Homology
BLAST of Sgr012749 vs. NCBI nr
Match:
XP_022156371.1 (phospholipase D zeta 2-like isoform X2 [Momordica charantia])
HSP 1 Score: 1818.9 bits (4710), Expect = 0.0e+00
Identity = 908/1110 (81.80%), Postives = 950/1110 (85.59%), Query Frame = 0
Query: 1 MSTERLIPSGTARSE-AEPPRLVSASHSFRQCGEPAWIFEELPVASIVSVSRPDTGDISP 60
MSTERLIP+GTA SE AEPPRLV+ASHSFRQCGEPA +FEELPVASIVSVSRPDTGDISP
Sbjct: 1 MSTERLIPNGTAPSEAAEPPRLVAASHSFRQCGEPARVFEELPVASIVSVSRPDTGDISP 60
Query: 61 LLLSYTIQIQYKQFKWSLVKKASQVLYLHFALKRRAFIEELHEKQEQVKEWLQNLGIVDH 120
LLLSYTIQIQYKQFKW+LVKKASQV+YLHFALKRR FIEELHEKQEQVKEWLQNLGI DH
Sbjct: 61 LLLSYTIQIQYKQFKWNLVKKASQVIYLHFALKRRTFIEELHEKQEQVKEWLQNLGIFDH 120
Query: 121 TAVVHNDDESDVGAFPLHDEQTTRNRNVPSVAALPIIKPALGGQRSISDKAKLAMQGYLN 180
TAVVHNDDESDV A DEQTTRNRNVPSVAALPIIKPALGGQRSISDKAKLAMQGYLN
Sbjct: 121 TAVVHNDDESDVAA----DEQTTRNRNVPSVAALPIIKPALGGQRSISDKAKLAMQGYLN 180
Query: 181 HFLGNLDIVNSRE----------------------------------------------- 240
HFLGNLDIVNSRE
Sbjct: 181 HFLGNLDIVNSREVCKFLEVSKMSFVREYGPKLKEGYLMVKHLKQLTGSDSSIKCCACHW 240
Query: 241 ----------VWAVLKPGFLALVADPLDTKLLDIIVFDVLPTLEGKEGSQANLAYHVKDR 300
VWAVLKPGFLALVA PLDTKLLDI+VFDVLP+LE KE SQA LA H+K+R
Sbjct: 241 CSCCDYNWKKVWAVLKPGFLALVAHPLDTKLLDIVVFDVLPSLEEKEESQAYLASHIKER 300
Query: 301 NPLRYSFMVRCGNGNMKFRTTSAAKVREWVTSINDAGLGPQDGWCHPHRFGSFAPQRGLS 360
NPLRYSF VRCGNGNMKFRTTS+AKVREWV+SINDAG GPQDGWCHPHRFGSFAPQRGLS
Sbjct: 301 NPLRYSFKVRCGNGNMKFRTTSSAKVREWVSSINDAGFGPQDGWCHPHRFGSFAPQRGLS 360
Query: 361 DDESRAQWFIDGRAAFEAIACSIEAAKSEIFITGWWLCPELYMQRPFHYHSSSRLDALLE 420
DDES+AQWFIDG AAFEAIACSIEAAKSEIFITGWWLCPELYM+RPF YH SSRLDALLE
Sbjct: 361 DDESQAQWFIDGGAAFEAIACSIEAAKSEIFITGWWLCPELYMRRPFQYHYSSRLDALLE 420
Query: 421 TKAKEGVKIYILIYKEVPIALKINSMYSKKRLLSIHENIKVLRSPDHMSTGIYYWSHHEK 480
TKAKEGVKIYIL+YKEVPIALKINSMYSK+RLL+IHENIKVLRSPDHMSTGIYYWSHHEK
Sbjct: 421 TKAKEGVKIYILMYKEVPIALKINSMYSKRRLLNIHENIKVLRSPDHMSTGIYYWSHHEK 480
Query: 481 LVIIDHHICFIGGLDLCFGRYDTMEHKVSDFPPYTWPGKDYYNPRESEPNSWEDTMKDEL 540
LVI+DHHICFIGGLDLCFGRYDTMEHKVSDFPPYTWPGKDYYNPRESEPNSWEDTMKDEL
Sbjct: 481 LVIVDHHICFIGGLDLCFGRYDTMEHKVSDFPPYTWPGKDYYNPRESEPNSWEDTMKDEL 540
Query: 541 EREKYPRMPWHDVHCALWGSPCRDVARHFVQRWNHAKRNKAPNEEKIPLLMPQHHMVLPH 600
+REKYPRMPWHDVHCALWGSPCRD+ARHFVQRWNHAKRNKAPNEEKIPLLMPQHHMVLPH
Sbjct: 541 DREKYPRMPWHDVHCALWGSPCRDIARHFVQRWNHAKRNKAPNEEKIPLLMPQHHMVLPH 600
Query: 601 YMGRSTELSFENNNSEQDHQKQTTVDFFSSLSPQQDIPLLLPQEVGGLPDSNIETNSLSM 660
YMGRSTELSFEN +SEQDHQKQTTVDF+SSLSPQQDIPLLLPQE GGLPD NIET+S SM
Sbjct: 601 YMGRSTELSFENKDSEQDHQKQTTVDFYSSLSPQQDIPLLLPQEAGGLPDLNIETSSPSM 660
Query: 661 NHNFLNKPMEIQRSVMDSFRTYIVEPLTQYDAQTNGLLDEFGFLDEFDALDHPREATIDA 720
NH+FLNKP+EIQR +MDS LTQY A+T G LDEFGFLDEF L HPREA IDA
Sbjct: 661 NHHFLNKPVEIQRRLMDS--------LTQY-AETYGSLDEFGFLDEFGDLGHPREAAIDA 720
Query: 721 PAYMKTSDNWLETEHESNYVVAIDDVKEIGPLTASNCQIIRSVSQWSAGTSKPEASIHAA 780
PAYMKTSD+WLETE ESN+VVA++DVKEIGP+T SNCQ+IRSVSQWSAGTSKPEASIH A
Sbjct: 721 PAYMKTSDDWLETEPESNHVVAVNDVKEIGPITTSNCQVIRSVSQWSAGTSKPEASIHGA 780
Query: 781 YCFAIQEAKHFIYIE--------------------------------------------- 840
YCFAIQEAKHFIYIE
Sbjct: 781 YCFAIQEAKHFIYIENQFFISGLSGDETIQNRVLEALYQRILLADKEQQCFRVIVVLPLL 840
Query: 841 ----GGLDDNGAATVRALMHWQYRSISWEKTSILYHLNLLLGPKTQDYILFCGLRSYGRL 900
GG+DDNGAATVRALMHWQYR+ISWEKTSILY LNLLLGPKTQDYILFCGLRSYGRL
Sbjct: 841 PGFQGGVDDNGAATVRALMHWQYRTISWEKTSILYRLNLLLGPKTQDYILFCGLRSYGRL 900
Query: 901 FDGGPIATSQVYVHSKLMIIDDCITFIGSSNINDRSLLGSRDSEIGVIIEDKEFVDSSMN 960
FDGGPIATSQVYVHSKLMIIDDCITFIGSSNINDRSLLGSRDSEIGV IEDKEFVDSSMN
Sbjct: 901 FDGGPIATSQVYVHSKLMIIDDCITFIGSSNINDRSLLGSRDSEIGVFIEDKEFVDSSMN 960
Query: 961 GKPWKAGKFAHSLRCSLWCEHLGLQLGEISQISDPTAEATYKDLWVATAKENTAIYKDVF 1004
GKPWKAGKFAHSLRCSLWCEHLGL+L E+SQISDP AEATYKD+W+ATAKENTAIY+DVF
Sbjct: 961 GKPWKAGKFAHSLRCSLWCEHLGLRLEEVSQISDPIAEATYKDIWLATAKENTAIYEDVF 1020
BLAST of Sgr012749 vs. NCBI nr
Match:
XP_038889503.1 (phospholipase D zeta 2-like [Benincasa hispida])
HSP 1 Score: 1807.0 bits (4679), Expect = 0.0e+00
Identity = 886/1109 (79.89%), Postives = 944/1109 (85.12%), Query Frame = 0
Query: 1 MSTERLIPSGTARSEAEPPRLVSASHSFRQCGEPAWIFEELPVASIVSVSRPDTGDISPL 60
MSTERLI SGT SEAEPP+L SASHSFRQCGEPA +FEELP+ASIVSVSRPDTGDISP
Sbjct: 1 MSTERLISSGTTPSEAEPPKLASASHSFRQCGEPARVFEELPIASIVSVSRPDTGDISPW 60
Query: 61 LLSYTIQIQYKQFKWSLVKKASQVLYLHFALKRRAFIEELHEKQEQVKEWLQNLGIVDHT 120
LLSYTI+IQYKQFKW LVK+ASQVLYLHFALKRRAFIEELHEKQEQVKEWL NLGIVDHT
Sbjct: 61 LLSYTIEIQYKQFKWRLVKRASQVLYLHFALKRRAFIEELHEKQEQVKEWLHNLGIVDHT 120
Query: 121 AVVHNDDESDVGAFPLHDEQTTRNRNVPSVAALPIIKPALGGQRSISDKAKLAMQGYLNH 180
AVVH+DDESD GAF HDEQTTRNRNVPSVAALPIIKPALGGQRSISDKAKLAMQGYLNH
Sbjct: 121 AVVHHDDESDDGAFSFHDEQTTRNRNVPSVAALPIIKPALGGQRSISDKAKLAMQGYLNH 180
Query: 181 FLGNLDIVNSRE------------------------------------------------ 240
F GNLDIVNSRE
Sbjct: 181 FFGNLDIVNSREVCKFLEVSKMSFVREHGPKLKEGYLMVKHLKQLTGTDSSINCFACHWC 240
Query: 241 ---------VWAVLKPGFLALVADPLDTKLLDIIVFDVLPTLEGKEGSQANLAYHVKDRN 300
VWAVLKPGFLALVADP+DTKLLDII+FDV+PTLE KE SQ LAY VK+RN
Sbjct: 241 SCCMYNWKKVWAVLKPGFLALVADPMDTKLLDIIIFDVIPTLEEKEESQTCLAYQVKERN 300
Query: 301 PLRYSFMVRCGNGNMKFRTTSAAKVREWVTSINDAGLGPQDGWCHPHRFGSFAPQRGLSD 360
PL YSF VRCG+G+M+FRTTS KV+EWV+SINDAG GPQDGWCHPHRFGSFAPQRGLSD
Sbjct: 301 PLHYSFKVRCGDGDMRFRTTSTTKVKEWVSSINDAGFGPQDGWCHPHRFGSFAPQRGLSD 360
Query: 361 DESRAQWFIDGRAAFEAIACSIEAAKSEIFITGWWLCPELYMQRPFHYHSSSRLDALLET 420
DES+AQWFIDGRAAFEAIACSIEAAKSEIFITGWWLCPELYM+RPFH HSSSRLDALLET
Sbjct: 361 DESQAQWFIDGRAAFEAIACSIEAAKSEIFITGWWLCPELYMRRPFHNHSSSRLDALLET 420
Query: 421 KAKEGVKIYILIYKEVPIALKINSMYSKKRLLSIHENIKVLRSPDHMSTGIYYWSHHEKL 480
KAKEGVKIYIL+YKEVPIALKINSMYSKKRL++IHENIKVLRSPDHMSTGIYYWSHHEK+
Sbjct: 421 KAKEGVKIYILMYKEVPIALKINSMYSKKRLVNIHENIKVLRSPDHMSTGIYYWSHHEKI 480
Query: 481 VIIDHHICFIGGLDLCFGRYDTMEHKVSDFPPYTWPGKDYYNPRESEPNSWEDTMKDELE 540
VI+DHHICFIGGLDLCFGRYDTMEHKVSDFPPYTWPGKDYYNPRESEPNSWEDTMKDELE
Sbjct: 481 VIVDHHICFIGGLDLCFGRYDTMEHKVSDFPPYTWPGKDYYNPRESEPNSWEDTMKDELE 540
Query: 541 REKYPRMPWHDVHCALWGSPCRDVARHFVQRWNHAKRNKAPNEEKIPLLMPQHHMVLPHY 600
REK PRMPWHDVHCALWGS CRD+ARHFVQRWNHAKRNKAPNEEKIPLLMPQH MVLPHY
Sbjct: 541 REKCPRMPWHDVHCALWGSSCRDIARHFVQRWNHAKRNKAPNEEKIPLLMPQHDMVLPHY 600
Query: 601 MGRSTELSFENNNSEQDHQKQTTVDFFSSLSPQQDIPLLLPQEVGGLPDSNIETNSLSMN 660
MG+ TELSF+N + EQDHQKQTT D FSSLSPQ+DIPLL+PQE+GGLPDSN++TNSLSMN
Sbjct: 601 MGKRTELSFKNKDLEQDHQKQTTDDVFSSLSPQEDIPLLMPQEIGGLPDSNVQTNSLSMN 660
Query: 661 HNFLNKPMEIQRSVMDSFRTYIVEPLTQYDAQTNGLLDEFGFLDEFDALDHPREATIDAP 720
HNF N+P EI+ SV DSF+ Y VEPLTQ++ QTNG LDEFGFLDEF A H REATID P
Sbjct: 661 HNFSNQPKEIRSSVRDSFQAYNVEPLTQFE-QTNGSLDEFGFLDEFGAFGHLREATIDTP 720
Query: 721 AYMKTSDNWLETEHESNYVVAIDDVKEIGPLTASNCQIIRSVSQWSAGTSKPEASIHAAY 780
YMKTS++WLE+EH S++V AI++VKEIGPLT SNCQIIRS+SQWSAGTSKPEASIHAAY
Sbjct: 721 PYMKTSNDWLESEHGSDHVAAINEVKEIGPLTTSNCQIIRSISQWSAGTSKPEASIHAAY 780
Query: 781 CFAIQEAKHFIYIE---------------------------------------------- 840
C AIQ+AKHFIY+E
Sbjct: 781 CSAIQKAKHFIYVENQFFISGLSGDETIQNRVLDALYQRIWLAHIEKQCFRVIIVLPLLP 840
Query: 841 ---GGLDDNGAATVRALMHWQYRSISWEKTSILYHLNLLLGPKTQDYILFCGLRSYGRLF 900
GG+DDNGAATVRALMHWQYR+ISWEKTSILY LNLLLGPKTQDYILFCGLRSYGRLF
Sbjct: 841 GFQGGVDDNGAATVRALMHWQYRTISWEKTSILYRLNLLLGPKTQDYILFCGLRSYGRLF 900
Query: 901 DGGPIATSQVYVHSKLMIIDDCITFIGSSNINDRSLLGSRDSEIGVIIEDKEFVDSSMNG 960
DGGPIATSQVYVHSKLMIIDDCITFIGSSNINDRSLLGSRDSEIGVIIEDKEFVDSSMNG
Sbjct: 901 DGGPIATSQVYVHSKLMIIDDCITFIGSSNINDRSLLGSRDSEIGVIIEDKEFVDSSMNG 960
Query: 961 KPWKAGKFAHSLRCSLWCEHLGLQLGEISQISDPTAEATYKDLWVATAKENTAIYKDVFS 1004
KPWKAGKFAHSLRCSLWCEHLGL LGE+SQI DP EATYKDLW+ATA+ENT IY+DVFS
Sbjct: 961 KPWKAGKFAHSLRCSLWCEHLGLHLGEVSQILDPIVEATYKDLWLATAQENTTIYEDVFS 1020
BLAST of Sgr012749 vs. NCBI nr
Match:
XP_022156370.1 (phospholipase D zeta 2-like isoform X1 [Momordica charantia])
HSP 1 Score: 1806.2 bits (4677), Expect = 0.0e+00
Identity = 905/1120 (80.80%), Postives = 949/1120 (84.73%), Query Frame = 0
Query: 1 MSTERLIPSGTARSE-AEPPRLVSASHSFRQCGEPAWIFEELPVASIVSVSRPDTGDISP 60
MSTERLIP+GTA SE AEPPRLV+ASHSFRQCGEPA +FEELPVASIVSVSRPDTGDISP
Sbjct: 1 MSTERLIPNGTAPSEAAEPPRLVAASHSFRQCGEPARVFEELPVASIVSVSRPDTGDISP 60
Query: 61 LLLSYTIQIQYKQFKWSLVKKASQVLYLHFALKRRAFIEELHEKQEQVKEWLQNLGIVDH 120
LLLSYTIQIQYKQFKW+LVKKASQV+YLHFALKRR FIEELHEKQEQVKEWLQNLGI DH
Sbjct: 61 LLLSYTIQIQYKQFKWNLVKKASQVIYLHFALKRRTFIEELHEKQEQVKEWLQNLGIFDH 120
Query: 121 TAVVHNDDESDVGAFPLHDEQTTRNRNVPSVAALPIIKPALGGQRSISDKAKLAMQGYLN 180
TAVVHNDDESDV A DEQTTRNRNVPSVAALPIIKPALGGQRSISDKAKLAMQGYLN
Sbjct: 121 TAVVHNDDESDVAA----DEQTTRNRNVPSVAALPIIKPALGGQRSISDKAKLAMQGYLN 180
Query: 181 HFLGNLDIVNSRE----------------------------------------------- 240
HFLGNLDIVNSRE
Sbjct: 181 HFLGNLDIVNSREVCKFLEVSKMSFVREYGPKLKEGYLMVKHLKQLTGSDSSIKCCACHW 240
Query: 241 ----------VWAVLKPGFLALVADPLDTKLLDIIVFDVLPTLEGKEGSQANLAYHVKDR 300
VWAVLKPGFLALVA PLDTKLLDI+VFDVLP+LE KE SQA LA H+K+R
Sbjct: 241 CSCCDYNWKKVWAVLKPGFLALVAHPLDTKLLDIVVFDVLPSLEEKEESQAYLASHIKER 300
Query: 301 NPLRYSFMVRCGNGNMKFRTTSAAKVREWVTSINDAGLGPQDGWCHPHRFGSFAPQRGLS 360
NPLRYSF VRCGNGNMKFRTTS+AKVREWV+SINDAG GPQDGWCHPHRFGSFAPQRGLS
Sbjct: 301 NPLRYSFKVRCGNGNMKFRTTSSAKVREWVSSINDAGFGPQDGWCHPHRFGSFAPQRGLS 360
Query: 361 DDESRAQWFIDGRAAFEAIACSIEAAKSEIFITGWWLCPELYMQRPFHYHSSSRLDALLE 420
DDES+AQWFIDG AAFEAIACSIEAAKSEIFITGWWLCPELYM+RPF YH SSRLDALLE
Sbjct: 361 DDESQAQWFIDGGAAFEAIACSIEAAKSEIFITGWWLCPELYMRRPFQYHYSSRLDALLE 420
Query: 421 TKAKEGVKIYILIYKEVPIALKINSMYSKKRLLSIHENIKVLRSPDHMSTGIYYWSHHEK 480
TKAKEGVKIYIL+YKEVPIALKINSMYSK+RLL+IHENIKVLRSPDHMSTGIYYWSHHEK
Sbjct: 421 TKAKEGVKIYILMYKEVPIALKINSMYSKRRLLNIHENIKVLRSPDHMSTGIYYWSHHEK 480
Query: 481 LVIIDHHICFIGGLDLCFGRYDTMEHKVSDFPPYTWPGKDYYNPRESEPNSWEDTMKDEL 540
LVI+DHHICFIGGLDLCFGRYDTMEHKVSDFPPYTWPGKDYYNPRESEPNSWEDTMKDEL
Sbjct: 481 LVIVDHHICFIGGLDLCFGRYDTMEHKVSDFPPYTWPGKDYYNPRESEPNSWEDTMKDEL 540
Query: 541 EREKYPRMPWHDVHCALWGSPCRDVARHFVQRWNHAKRNKAPNEEKIPLLMPQHHMVLPH 600
+REKYPRMPWHDVHCALWGSPCRD+ARHFVQRWNHAKRNKAPNEEKIPLLMPQHHMVLPH
Sbjct: 541 DREKYPRMPWHDVHCALWGSPCRDIARHFVQRWNHAKRNKAPNEEKIPLLMPQHHMVLPH 600
Query: 601 YMGRSTELSFENNNSEQDHQKQTTVDFFSSLSPQQDIPLLLPQEVGGLPDSNIETNSLSM 660
YMGRSTELSFEN +SEQDHQKQTTVDF+SSLSPQQDIPLLLPQE GGLPD NIET+S SM
Sbjct: 601 YMGRSTELSFENKDSEQDHQKQTTVDFYSSLSPQQDIPLLLPQEAGGLPDLNIETSSPSM 660
Query: 661 NHNFLNKPMEIQRSVMDSFRTYIVEPLTQYDAQTNGLLDEFGFLDEFDALDHPREATIDA 720
NH+FLNKP+EIQR +MDS LTQY A+T G LDEFGFLDEF L HPREA IDA
Sbjct: 661 NHHFLNKPVEIQRRLMDS--------LTQY-AETYGSLDEFGFLDEFGDLGHPREAAIDA 720
Query: 721 PAYMKTSDNWLETEHESNYVVAIDDVKEIGPLTASNCQIIRSVSQWSAGTSKPEASIHAA 780
PAYMKTSD+WLETE ESN+VVA++DVKEIGP+T SNCQ+IRSVSQWSAGTSKPEASIH A
Sbjct: 721 PAYMKTSDDWLETEPESNHVVAVNDVKEIGPITTSNCQVIRSVSQWSAGTSKPEASIHGA 780
Query: 781 YCFAIQEAKHFIYIE--------------------------------------------- 840
YCFAIQEAKHFIYIE
Sbjct: 781 YCFAIQEAKHFIYIENQFFISGLSGDETIQNRVLEALYQRILLADKEQQCFRVIVVLPLL 840
Query: 841 ----GGLDDNGAATVRALMHWQYRSISWEKTSILYHLNLLLGPKTQDYILFCGLRSYGRL 900
GG+DDNGAATVRALMHWQYR+ISWEKTSILY LNLLLGPKTQDYILFCGLRSYGRL
Sbjct: 841 PGFQGGVDDNGAATVRALMHWQYRTISWEKTSILYRLNLLLGPKTQDYILFCGLRSYGRL 900
Query: 901 FDGGPIATSQVYVHSKLMIIDDCITFIGSSNINDRSLLGSRDSEIGVIIEDKEFVDSSMN 960
FDGGPIATSQVYVHSKLMIIDDCITFIGSSNINDRSLLGSRDSEIGV IEDKEFVDSSMN
Sbjct: 901 FDGGPIATSQVYVHSKLMIIDDCITFIGSSNINDRSLLGSRDSEIGVFIEDKEFVDSSMN 960
Query: 961 GKPWKAGKFAHSLRCSLWCEHLGLQLGEISQISDPTAEATYKDLWVATAKENTAIYKDVF 1014
GKPWKAGKFAHSLRCSLWCEHLGL+L E+SQISDP AEATYKD+W+ATAKENTAIY+DVF
Sbjct: 961 GKPWKAGKFAHSLRCSLWCEHLGLRLEEVSQISDPIAEATYKDIWLATAKENTAIYEDVF 1020
BLAST of Sgr012749 vs. NCBI nr
Match:
XP_022946834.1 (phospholipase D zeta 2 [Cucurbita moschata])
HSP 1 Score: 1782.3 bits (4615), Expect = 0.0e+00
Identity = 883/1109 (79.62%), Postives = 934/1109 (84.22%), Query Frame = 0
Query: 1 MSTERLIPSGTARSEAEPPRLVSASHSFRQCGEPAWIFEELPVASIVSVSRPDTGDISPL 60
MSTERLI SGT SEAE PRL S+SHSFRQCGEPA +FEELP+ASIVSVSRPDTGDISPL
Sbjct: 1 MSTERLISSGTTPSEAEAPRLASSSHSFRQCGEPARVFEELPIASIVSVSRPDTGDISPL 60
Query: 61 LLSYTIQIQYKQFKWSLVKKASQVLYLHFALKRRAFIEELHEKQEQVKEWLQNLGIVDHT 120
LLSYTI+IQYKQFKWSLVKKASQVLYLHFALKRRAFIEELHEKQEQVKEWL NLGIVDHT
Sbjct: 61 LLSYTIEIQYKQFKWSLVKKASQVLYLHFALKRRAFIEELHEKQEQVKEWLHNLGIVDHT 120
Query: 121 AVVHNDDESDVGAFPLHDEQTTRNRNVPSVAALPIIKPALGGQRSISDKAKLAMQGYLNH 180
A VH+DDESD G FPLHDEQTT+NRNVPSVAALPIIKPA+GGQRSISDKAKLAMQGYLNH
Sbjct: 121 AAVHHDDESDDGLFPLHDEQTTKNRNVPSVAALPIIKPAMGGQRSISDKAKLAMQGYLNH 180
Query: 181 FLGNLDIVNSRE------------------------------------------------ 240
F GNLDIVN+RE
Sbjct: 181 FFGNLDIVNTREVCKFLEVSKMSFVREHGPKLKEGYLMVKHLKQLTGSDSSIKCFACHWC 240
Query: 241 ---------VWAVLKPGFLALVADPLDTKLLDIIVFDVLPTLEGKEGSQANLAYHVKDRN 300
VWAVLKPGFLALVADP+DTKLLDIIVFDVL TLE KEGSQA LAYHVK+RN
Sbjct: 241 SCCVYNWKKVWAVLKPGFLALVADPMDTKLLDIIVFDVLQTLEEKEGSQACLAYHVKERN 300
Query: 301 PLRYSFMVRCGNGNMKFRTTSAAKVREWVTSINDAGLGPQDGWCHPHRFGSFAPQRGLSD 360
PLRYSF VR GNGN++FRTTS AKV++WV++INDAG G +DGWCHPHRFGSFAPQRGLSD
Sbjct: 301 PLRYSFKVRGGNGNLRFRTTSTAKVKKWVSAINDAGFGSKDGWCHPHRFGSFAPQRGLSD 360
Query: 361 DESRAQWFIDGRAAFEAIACSIEAAKSEIFITGWWLCPELYMQRPFHYHSSSRLDALLET 420
DES+AQWFIDGRAAFEAIACSIEAAKSEIFITGWWLCPELYM+RPFH HSSSRLDALLET
Sbjct: 361 DESQAQWFIDGRAAFEAIACSIEAAKSEIFITGWWLCPELYMRRPFHNHSSSRLDALLET 420
Query: 421 KAKEGVKIYILIYKEVPIALKINSMYSKKRLLSIHENIKVLRSPDHMSTGIYYWSHHEKL 480
KAKEGVKIYIL+YKEVPIALKINS YSKKRLL+IHENIKVLRSPDHMSTGIYYWSHHEKL
Sbjct: 421 KAKEGVKIYILMYKEVPIALKINSRYSKKRLLNIHENIKVLRSPDHMSTGIYYWSHHEKL 480
Query: 481 VIIDHHICFIGGLDLCFGRYDTMEHKVSDFPPYTWPGKDYYNPRESEPNSWEDTMKDELE 540
VI+DHHICFIGGLDLCFGRYDTMEHKVSDFPPYTWPGKDYYNPRESEPNSWEDTM DELE
Sbjct: 481 VIVDHHICFIGGLDLCFGRYDTMEHKVSDFPPYTWPGKDYYNPRESEPNSWEDTMSDELE 540
Query: 541 REKYPRMPWHDVHCALWGSPCRDVARHFVQRWNHAKRNKAPNEEKIPLLMPQHHMVLPHY 600
REK PRMPWHDVHCALWGSPCRD+ARHFVQRWNHAKR+KAPNEEKIPLLMPQH MVLPHY
Sbjct: 541 REKCPRMPWHDVHCALWGSPCRDIARHFVQRWNHAKRDKAPNEEKIPLLMPQHDMVLPHY 600
Query: 601 MGRSTELSFENNNSEQDHQKQTTVDFFSSLSPQQDIPLLLPQEVGGLPDSNIETNSLSMN 660
MGRSTELSF+N SEQDHQKQ T DFF S SPQ+DIPLL+PQEVGGLPDSN+E NS
Sbjct: 601 MGRSTELSFKNEGSEQDHQKQNTEDFFFSSSPQEDIPLLMPQEVGGLPDSNMERNSQ--- 660
Query: 661 HNFLNKPMEIQRSVMDSFRTYIVEPLTQYDAQTNGLLDEFGFLDEFDALDHPREATIDAP 720
+NK MEIQ S MDSF+ Y VE LTQY+ Q NGL DEFGFLDEF REAT D P
Sbjct: 661 ---MNKQMEIQSSAMDSFQAYNVESLTQYE-QANGLFDEFGFLDEFGDFGLSREATTDTP 720
Query: 721 AYMKTSDNWLETEHESNYVVAIDDVKEIGPLTASNCQIIRSVSQWSAGTSKPEASIHAAY 780
YMKTSD+WLETEHESN+VVA+++VKEIGPLT +NCQ+IRSVSQWSAGTSKPEASIHAAY
Sbjct: 721 PYMKTSDDWLETEHESNHVVAVNEVKEIGPLTTTNCQVIRSVSQWSAGTSKPEASIHAAY 780
Query: 781 CFAIQEAKHFIYIE---------------------------------------------- 840
C AIQEAKHFIYIE
Sbjct: 781 CSAIQEAKHFIYIENQFFISGLSGDETIHNRVLEALYQRIWLAHKEKQCFRVIIVLPLLP 840
Query: 841 ---GGLDDNGAATVRALMHWQYRSISWEKTSILYHLNLLLGPKTQDYILFCGLRSYGRLF 900
GG+DDNGAATVRALMHWQYR+ISWEKTSILY LNLLLG KTQDYILFCGLRSYGRLF
Sbjct: 841 GFQGGVDDNGAATVRALMHWQYRTISWEKTSILYRLNLLLGSKTQDYILFCGLRSYGRLF 900
Query: 901 DGGPIATSQVYVHSKLMIIDDCITFIGSSNINDRSLLGSRDSEIGVIIEDKEFVDSSMNG 960
DGGPIATSQVYVHSKLMIIDDCITFIGSSNINDRSLLGSRDSEIGVIIEDKEFVDS MNG
Sbjct: 901 DGGPIATSQVYVHSKLMIIDDCITFIGSSNINDRSLLGSRDSEIGVIIEDKEFVDSYMNG 960
Query: 961 KPWKAGKFAHSLRCSLWCEHLGLQLGEISQISDPTAEATYKDLWVATAKENTAIYKDVFS 1004
KPWKAGKFAHSLRCSLWCEHLGL LGE+SQI DP EATYKDLW+ATAKENTAIY+DVFS
Sbjct: 961 KPWKAGKFAHSLRCSLWCEHLGLHLGEVSQILDPIVEATYKDLWIATAKENTAIYEDVFS 1020
BLAST of Sgr012749 vs. NCBI nr
Match:
XP_022999585.1 (phospholipase D zeta 2 [Cucurbita maxima])
HSP 1 Score: 1778.8 bits (4606), Expect = 0.0e+00
Identity = 882/1109 (79.53%), Postives = 932/1109 (84.04%), Query Frame = 0
Query: 1 MSTERLIPSGTARSEAEPPRLVSASHSFRQCGEPAWIFEELPVASIVSVSRPDTGDISPL 60
MSTERLI SGT SEAEPPRL S+SHSFRQCGE A +FEELP+ASIVSVSRPDTGDISPL
Sbjct: 1 MSTERLISSGTTPSEAEPPRLASSSHSFRQCGESARVFEELPIASIVSVSRPDTGDISPL 60
Query: 61 LLSYTIQIQYKQFKWSLVKKASQVLYLHFALKRRAFIEELHEKQEQVKEWLQNLGIVDHT 120
LLSYTI+IQYKQF WSLVKKASQVLYLHFALKRRAFIEELHEKQEQVKEWL NLGIVDHT
Sbjct: 61 LLSYTIEIQYKQFIWSLVKKASQVLYLHFALKRRAFIEELHEKQEQVKEWLHNLGIVDHT 120
Query: 121 AVVHNDDESDVGAFPLHDEQTTRNRNVPSVAALPIIKPALGGQRSISDKAKLAMQGYLNH 180
A VH+DDESD G FPLHDEQTT+NRNVPSVAALPIIKPA+GGQRSISDKAKLAMQGYLNH
Sbjct: 121 AAVHHDDESDDGLFPLHDEQTTKNRNVPSVAALPIIKPAMGGQRSISDKAKLAMQGYLNH 180
Query: 181 FLGNLDIVNSRE------------------------------------------------ 240
F GNLDIVN+RE
Sbjct: 181 FFGNLDIVNTREVCKFLEVSKMSFVREHGPKLKEGYLMVKHLKQLTGSDSSIKCFACHWC 240
Query: 241 ---------VWAVLKPGFLALVADPLDTKLLDIIVFDVLPTLEGKEGSQANLAYHVKDRN 300
VWAVLKPGFLALVADP+DTKLLDIIVFDVLPTLE KEGSQA LAYHVK+RN
Sbjct: 241 SCCVYNWKKVWAVLKPGFLALVADPMDTKLLDIIVFDVLPTLEEKEGSQACLAYHVKERN 300
Query: 301 PLRYSFMVRCGNGNMKFRTTSAAKVREWVTSINDAGLGPQDGWCHPHRFGSFAPQRGLSD 360
PLRYSF VR GNGNM+FRTTS AKV++WV++INDAG G +DGWCHPHRFGSFAPQRGLSD
Sbjct: 301 PLRYSFKVRGGNGNMRFRTTSTAKVKKWVSAINDAGFGSKDGWCHPHRFGSFAPQRGLSD 360
Query: 361 DESRAQWFIDGRAAFEAIACSIEAAKSEIFITGWWLCPELYMQRPFHYHSSSRLDALLET 420
DES+AQWFIDGRAAFEAIACSIEAAKSEIFITGWWLCPELY++RPFH HSSSRLDALLET
Sbjct: 361 DESQAQWFIDGRAAFEAIACSIEAAKSEIFITGWWLCPELYLRRPFHNHSSSRLDALLET 420
Query: 421 KAKEGVKIYILIYKEVPIALKINSMYSKKRLLSIHENIKVLRSPDHMSTGIYYWSHHEKL 480
KAKEGVKIYIL+YKEVPIALKINS YSKKRLL+IHENIKVLRSPDHMSTGIYYWSHHEKL
Sbjct: 421 KAKEGVKIYILMYKEVPIALKINSRYSKKRLLNIHENIKVLRSPDHMSTGIYYWSHHEKL 480
Query: 481 VIIDHHICFIGGLDLCFGRYDTMEHKVSDFPPYTWPGKDYYNPRESEPNSWEDTMKDELE 540
VI+DHHICFIGGLDLCFGRYDTMEHKVSDFPPYTWPGKDYYNPRESEPNSWEDTM DELE
Sbjct: 481 VIVDHHICFIGGLDLCFGRYDTMEHKVSDFPPYTWPGKDYYNPRESEPNSWEDTMSDELE 540
Query: 541 REKYPRMPWHDVHCALWGSPCRDVARHFVQRWNHAKRNKAPNEEKIPLLMPQHHMVLPHY 600
REK PRMPWHDVHCALWGSPCRD+ARHFVQRWNHAKR+KAPNEEKIPLLMPQH MVLPHY
Sbjct: 541 REKCPRMPWHDVHCALWGSPCRDIARHFVQRWNHAKRDKAPNEEKIPLLMPQHDMVLPHY 600
Query: 601 MGRSTELSFENNNSEQDHQKQTTVDFFSSLSPQQDIPLLLPQEVGGLPDSNIETNSLSMN 660
MGRSTELSF+N SEQDHQKQ T DFF S SPQ+DIPLL+PQEVGGLP SN+E NS
Sbjct: 601 MGRSTELSFKNKGSEQDHQKQNTEDFFFSSSPQEDIPLLMPQEVGGLPVSNMERNSQ--- 660
Query: 661 HNFLNKPMEIQRSVMDSFRTYIVEPLTQYDAQTNGLLDEFGFLDEFDALDHPREATIDAP 720
+NK MEIQ S MDSF+ Y VE LTQY+ Q NGL DEFGFLDEF REAT D P
Sbjct: 661 ---MNKQMEIQSSAMDSFQAYNVESLTQYE-QANGLFDEFGFLDEFGDFGLSREATTDTP 720
Query: 721 AYMKTSDNWLETEHESNYVVAIDDVKEIGPLTASNCQIIRSVSQWSAGTSKPEASIHAAY 780
YMKTSD+WLETEHESN+VVA+++VKEIGPLT +NCQ+IRSVSQWSAGTSKPEASIHAAY
Sbjct: 721 TYMKTSDDWLETEHESNHVVAVNEVKEIGPLTTTNCQVIRSVSQWSAGTSKPEASIHAAY 780
Query: 781 CFAIQEAKHFIYIE---------------------------------------------- 840
C AIQEAKHFIYIE
Sbjct: 781 CSAIQEAKHFIYIENQFFISGLSGDETIHNRVLETLYQRIWLAHKEKQCFRVIIVLPLLP 840
Query: 841 ---GGLDDNGAATVRALMHWQYRSISWEKTSILYHLNLLLGPKTQDYILFCGLRSYGRLF 900
GG+DDNGAATVRALMHWQYR+ISWEKTSILY LNLLLG KTQDYILFCGLRSYGRLF
Sbjct: 841 GFQGGVDDNGAATVRALMHWQYRTISWEKTSILYRLNLLLGSKTQDYILFCGLRSYGRLF 900
Query: 901 DGGPIATSQVYVHSKLMIIDDCITFIGSSNINDRSLLGSRDSEIGVIIEDKEFVDSSMNG 960
DGGPIATSQVYVHSKLMIIDDCITFIGSSNINDRSLLGSRDSEIGVIIEDKEFVDS MNG
Sbjct: 901 DGGPIATSQVYVHSKLMIIDDCITFIGSSNINDRSLLGSRDSEIGVIIEDKEFVDSCMNG 960
Query: 961 KPWKAGKFAHSLRCSLWCEHLGLQLGEISQISDPTAEATYKDLWVATAKENTAIYKDVFS 1004
KPWKAGKFAHSLRCSLWCEHLGL LGE+SQI DP EATYKDLW+ATAKENTAIY+DVFS
Sbjct: 961 KPWKAGKFAHSLRCSLWCEHLGLHLGEVSQILDPIVEATYKDLWIATAKENTAIYEDVFS 1020
BLAST of Sgr012749 vs. ExPASy Swiss-Prot
Match:
Q9LRZ5 (Phospholipase D zeta 1 OS=Arabidopsis thaliana OX=3702 GN=PLDZETA1 PE=1 SV=1)
HSP 1 Score: 1215.7 bits (3144), Expect = 0.0e+00
Identity = 629/1097 (57.34%), Postives = 749/1097 (68.28%), Query Frame = 0
Query: 19 PRLVSASHSFRQC--GEPAWIFEELPVASIVSVSRPDTGDISPLLLSYTIQIQYKQFKWS 78
P +VS+ SF E IFEELP A IVSVSRPD GDISP+LLSYTI+ QYKQFKW
Sbjct: 25 PSMVSSLFSFAPAPTQETNRIFEELPKAVIVSVSRPDAGDISPVLLSYTIECQYKQFKWQ 84
Query: 79 LVKKASQVLYLHFALKRRAFIEELHEKQEQVKEWLQNLGIVDHTAVVHNDDESDVGAFPL 138
LVKKASQV YLHFALK+RAFIEE+HEKQEQVKEWLQNLGI DH VV ++D +V PL
Sbjct: 85 LVKKASQVFYLHFALKKRAFIEEIHEKQEQVKEWLQNLGIGDHPPVVQDEDADEV---PL 144
Query: 139 HDEQTTRNRNVPSVAALPIIKPALGGQRSISDKAKLAMQGYLNHFLGNLDIVNSRE---- 198
H +++ +NR+VPS AALP+I+P LG Q+SIS + K AMQ YLNHFLGNLDIVNSRE
Sbjct: 145 HQDESAKNRDVPSSAALPVIRP-LGRQQSISVRGKHAMQEYLNHFLGNLDIVNSREVCRF 204
Query: 199 ------------------------------------------------------VWAVLK 258
VW VLK
Sbjct: 205 LEVSMLSFSPEYGPKLKEDYIMVKHLPKFSKSDDDSNRCCGCCWFCCCNDNWQKVWGVLK 264
Query: 259 PGFLALVADPLDTKLLDIIVFDVLPTLEGKEGSQANLAYHVKDRNPLRYSFMVRCGNGNM 318
PGFLAL+ DP D KLLDIIVFDVLP G +G +LA +KD NPLR++F V GN ++
Sbjct: 265 PGFLALLEDPFDAKLLDIIVFDVLPVSNGNDGVDISLAVELKDHNPLRHAFKVTSGNRSI 324
Query: 319 KFRTTSAAKVREWVTSINDAGLGPQDGWCHPHRFGSFAPQRGLSDDESRAQWFIDGRAAF 378
+ R ++AKV++WV SINDA L P +GWCHPHRFGS+AP RGL+DD S+AQWF+DG AAF
Sbjct: 325 RIRAKNSAKVKDWVASINDAALRPPEGWCHPHRFGSYAPPRGLTDDGSQAQWFVDGGAAF 384
Query: 379 EAIACSIEAAKSEIFITGWWLCPELYMQRPFHYHSSSRLDALLETKAKEGVKIYILIYKE 438
AIA +IE AKSEIFI GWW+CPELY++RPF H+SSRLD LLE KAK+GV+IYILIYKE
Sbjct: 385 AAIAAAIENAKSEIFICGWWVCPELYLRRPFDPHTSSRLDNLLENKAKQGVQIYILIYKE 444
Query: 439 VPIALKINSMYSKKRLLSIHENIKVLRSPDHMSTGIYYWSHHEKLVIIDHHICFIGGLDL 498
V +ALKINS+YSK+RLL IHEN++VLR PDH S+G+Y WSHHEKLVI+D+ +CFIGGLDL
Sbjct: 445 VALALKINSVYSKRRLLGIHENVRVLRYPDHFSSGVYLWSHHEKLVIVDNQVCFIGGLDL 504
Query: 499 CFGRYDTMEHKVSDFPPYTWPGKDYYNPRESEPNSWEDTMKDELEREKYPRMPWHDVHCA 558
CFGRYDT EHKV D P TWPGKDYYNPRESEPN+WED +KDELER+K+PRMPWHDVHCA
Sbjct: 505 CFGRYDTFEHKVGDNPSVTWPGKDYYNPRESEPNTWEDALKDELERKKHPRMPWHDVHCA 564
Query: 559 LWGSPCRDVARHFVQRWNHAKRNKAPNEEKIPLLMPQHHMVLPHYMGRSTELSFENNNSE 618
LWG PCRDVARHFVQRWN+AKRNKAP E+ IPLLMPQHHMV+PHYMGR E E+ E
Sbjct: 565 LWGPPCRDVARHFVQRWNYAKRNKAPYEDSIPLLMPQHHMVIPHYMGRQEESDIESKKEE 624
Query: 619 QDHQKQTTVDFFSSLSPQQDIPLLLPQE---VGGLPDSNIETNSLSMNHNFLNKPMEIQR 678
+ D FSS S QDIPLLLP E G + E + + N F
Sbjct: 625 DSIRGIRRDDSFSSRSSLQDIPLLLPHEPVDQDGSSGGHKENGTNNRNGPF--------- 684
Query: 679 SVMDSFRTYIVEPLTQYDAQTNGLLDEFGFLDEFDALDHPREATIDAPAYMKTSDNWLET 738
SFR +EP+ +G GF+D+ + LD P + W ET
Sbjct: 685 ----SFRKSKIEPV-------DGDTPMRGFVDDRNGLDLP----VAKRGSNAIDSEWWET 744
Query: 739 EHESNYVVAIDDVKEIGPLTASNCQIIRSVSQWSAGTSKPEASIHAAYCFAIQEAKHFIY 798
+ V + D+ ++GP T+ CQIIRSVSQWSAGTS+ E SIH+AY I +A+HFIY
Sbjct: 745 QDHDYQVGSPDETGQVGPRTSCRCQIIRSVSQWSAGTSQVEESIHSAYRSLIDKAEHFIY 804
Query: 799 IE-------------------------------------------------GGLDDNGAA 858
IE GG+DD+GAA
Sbjct: 805 IENQFFISGLSGDDTVKNRVLEALYKRILRAHNEKKIFRVVVVIPLLPGFQGGIDDSGAA 864
Query: 859 TVRALMHWQYRSISWEKTSILYHLNLLLGPKTQDYILFCGLRSYGRLFDGGPIATSQVYV 918
+VRA+MHWQYR+I SIL +L +G K DYI F GLR+YG+L + GP+ATSQVYV
Sbjct: 865 SVRAIMHWQYRTIYRGHNSILTNLYNTIGVKAHDYISFYGLRAYGKLSEDGPVATSQVYV 924
Query: 919 HSKLMIIDDCITFIGSSNINDRSLLGSRDSEIGVIIEDKEFVDSSMNGKPWKAGKFAHSL 978
HSK+MI+DD IGS+NINDRSLLGSRDSEIGV+IED E VDS M GKPWKAGKF+ SL
Sbjct: 925 HSKIMIVDDRAALIGSANINDRSLLGSRDSEIGVLIEDTELVDSRMAGKPWKAGKFSSSL 984
Query: 979 RCSLWCEHLGLQLGEISQISDPTAEATYKDLWVATAKENTAIYKDVFSCIPNDNINSRSS 1004
R SLW EHLGL+ GEI QI DP +++TYK++W+ATAK NT IY+DVFSC+PND I+SR +
Sbjct: 985 RLSLWSEHLGLRTGEIDQIIDPVSDSTYKEIWMATAKTNTMIYQDVFSCVPNDLIHSRMA 1044
BLAST of Sgr012749 vs. ExPASy Swiss-Prot
Match:
Q9M9W8 (Phospholipase D zeta 2 OS=Arabidopsis thaliana OX=3702 GN=PLPZETA2 PE=1 SV=2)
HSP 1 Score: 1154.4 bits (2985), Expect = 0.0e+00
Identity = 627/1124 (55.78%), Postives = 734/1124 (65.30%), Query Frame = 0
Query: 1 MSTERLI------PSGTARSEAEPPRLVSASHSFRQCGEPAWIFEELPVASIVSVSRPDT 60
MST++L+ G R +AS S G + IF+ELP A+IVSVSRPDT
Sbjct: 1 MSTDKLLLPNGVKSDGVIRMTRADAAAAAASSSL---GGGSQIFDELPKAAIVSVSRPDT 60
Query: 61 GDISPLLLSYTIQIQYKQFKWSLVKKASQVLYLHFALKRRAFIEELHEKQEQVKEWLQNL 120
D SPLLLSYT+++QYKQFKW+L KKASQVLYLHFALK+R IEELH+KQEQV+EWL +L
Sbjct: 61 TDFSPLLLSYTLELQYKQFKWTLQKKASQVLYLHFALKKRLIIEELHDKQEQVREWLHSL 120
Query: 121 GIVD-HTAVVHNDDESDVGAFPLH-DEQTTRNRNVPSVAALPIIKPALGGQRSISDKAKL 180
GI D +VV +D+E D GA PLH E + +NRNVPS AALPII+P +G ++ D+ +
Sbjct: 121 GIFDMQGSVVQDDEEPDDGALPLHYTEDSIKNRNVPSRAALPIIRPTIGRSETVVDRGRT 180
Query: 181 AMQGYLNHFLGNLDIVNSRE---------------------------------------- 240
AMQGYL+ FLGNLDIVNS+E
Sbjct: 181 AMQGYLSLFLGNLDIVNSKEVCKFLEVSRLSFAREYGSKMKEGYVTVKHLRDVPGSDGVR 240
Query: 241 -----------------VWAVLKPGFLALVADPLDTKLLDIIVFDVLPTLEGKEGS-QAN 300
VWAVLKPGFLAL+ DP KLLDI+VFD L KE S Q
Sbjct: 241 CCLPTHCLGFFGTSWTKVWAVLKPGFLALLEDPFSGKLLDIMVFDTLGLQGTKESSEQPR 300
Query: 301 LAYHVKDRNPLRYSFMVRCGNGNMKFRTTSAAKVREWVTSINDAGLGPQDGWCH-PHRFG 360
LA VK+ NPLR+ F V G+ ++ RTTS+ KV+EWV ++++AG C+ PHRFG
Sbjct: 301 LAEQVKEHNPLRFGFKVTSGDRTVRLRTTSSRKVKEWVKAVDEAG-------CYSPHRFG 360
Query: 361 SFAPQRGLSDDESRAQWFIDGRAAFEAIACSIEAAKSEIFITGWWLCPELYMQRPFHYHS 420
SFAP RGL+ D S+AQWF+DG AFEAIA +I+ A SEIF+TGWWLCPELY++RPF H
Sbjct: 361 SFAPPRGLTSDGSQAQWFVDGHTAFEAIAFAIQNATSEIFMTGWWLCPELYLKRPFEDHP 420
Query: 421 SSRLDALLETKAKEGVKIYILIYKEVPIALKINSMYSKKRLLSIHENIKVLRSPDHMSTG 480
S RLDALLETKAK+GVKIYIL+YKEV IALKINS+YSKKRL +IH+N+KVLR PDH+S+G
Sbjct: 421 SLRLDALLETKAKQGVKIYILLYKEVQIALKINSLYSKKRLQNIHKNVKVLRYPDHLSSG 480
Query: 481 IYYWSHHEKLVIIDHHICFIGGLDLCFGRYDTMEHKVSDFPPYTWPGKDYYNPRESEPNS 540
IY WSHHEK+VI+D+ +CFIGGLDLCFGRYDT EHK+ D PPY WPGKDYYNPRESEPNS
Sbjct: 481 IYLWSHHEKIVIVDYQVCFIGGLDLCFGRYDTAEHKIGDCPPYIWPGKDYYNPRESEPNS 540
Query: 541 WEDTMKDELEREKYPRMPWHDVHCALWGSPCRDVARHFVQRWNHAKRNKAPNEEKIPLLM 600
WE+TMKDEL+R KYPRMPWHDVHCALWG PCRDVARHFVQRWNH+KRNKAPNE+ IPLLM
Sbjct: 541 WEETMKDELDRRKYPRMPWHDVHCALWGPPCRDVARHFVQRWNHSKRNKAPNEQTIPLLM 600
Query: 601 PQHHMVLPHYMGRSTELSFENNNSEQDHQKQTTV---DFFSSLSPQQDIPLLLPQEVGGL 660
P HHMVLPHY+G E+D K + D FSS SP Q+IPLLLPQE
Sbjct: 601 PHHHMVLPHYLGTREIDIIAAAKPEEDPDKPVVLARHDSFSSASPPQEIPLLLPQE---- 660
Query: 661 PDSNIETNSLSMNHNFLNKPMEIQRSVMDSFRTYIVEPLTQYDAQTNGLLDEFGFLDEFD 720
T D G
Sbjct: 661 ---------------------------------------TDADFAGRG------------ 720
Query: 721 ALDHPREATIDAPAYMKTSDNWLETEHESNYVVAIDD-VKEIGPLTASNCQIIRSVSQWS 780
+ +D+ A ET ES+ A++D +IG + CQIIRSVSQWS
Sbjct: 721 ------DLKLDSGARQDPG----ETSEESDLDEAVNDWWWQIGKQSDCRCQIIRSVSQWS 780
Query: 781 AGTSKPEASIHAAYCFAIQEAKHFIYIE-------------------------------- 840
AGTS+PE SIH AYC IQ A+HFIYIE
Sbjct: 781 AGTSQPEDSIHRAYCSLIQNAEHFIYIENQFFISGLEKEDTILNRVLEALYRRILKAHEE 840
Query: 841 -----------------GGLDDNGAATVRALMHWQYRSISWEKTSILYHLNLLLGPKTQD 900
GG+DD GAATVRALMHWQYR+IS E TSIL +LN LLGPKTQD
Sbjct: 841 NKCFRVVIVIPLLPGFQGGIDDFGAATVRALMHWQYRTISREGTSILDNLNALLGPKTQD 900
Query: 901 YILFCGLRSYGRLFDGGPIATSQVYVHSKLMIIDDCITFIGSSNINDRSLLGSRDSEIGV 960
YI F GLRSYGRLF+ GPIATSQ+YVHSKLMI+DD I IGSSNINDRSLLGSRDSEIGV
Sbjct: 901 YISFYGLRSYGRLFEDGPIATSQIYVHSKLMIVDDRIAVIGSSNINDRSLLGSRDSEIGV 960
Query: 961 IIEDKEFVDSSMNGKPWKAGKFAHSLRCSLWCEHLGLQLGEISQISDPTAEATYKDLWVA 1004
+IEDKEFV+SSMNG W AGKF++SLRCSLW EHLGL GEI +I DP +ATYKDLW+A
Sbjct: 961 VIEDKEFVESSMNGMKWMAGKFSYSLRCSLWSEHLGLHAGEIQKIEDPIKDATYKDLWMA 1020
BLAST of Sgr012749 vs. ExPASy Swiss-Prot
Match:
Q0V8L6 (Phospholipase D2 OS=Bos taurus OX=9913 GN=PLD2 PE=2 SV=1)
HSP 1 Score: 354.4 bits (908), Expect = 5.0e-96
Identity = 254/837 (30.35%), Postives = 390/837 (46.59%), Query Frame = 0
Query: 194 WAVLKPGFLALVADPLDTKLLDII-VFDVLPTLEGKEGSQANLAYHVKDRNPLRYSFMVR 253
W V+K FL + L+T + + +FD P + + G ++ A RY V
Sbjct: 237 WLVVKDSFLLYMC--LETGAISFVQLFD--PGFKVQVGKRSTEA---------RYGVRVD 296
Query: 254 CGNGNMKFRTTSAAKVREWVTSINDAGLGPQDGWCHPHRFGSFAPQRGLSDDESRAQWFI 313
+ ++ + +S + R W I + GP + HR S+AP R + A+WF+
Sbjct: 297 TSHRSLILKCSSYRQARWWAQEITELAQGPGRDFIQLHRHDSYAPPR----PGTLARWFV 356
Query: 314 DGRAAFEAIACSIEAAKSEIFITGWWLCPELYMQRPFHYHSSSRLDALLETKAKEGVKIY 373
+G F A+A +I A+ EIFIT WWL PE+Y++RP H RLD +L+ KA+EGV +
Sbjct: 357 NGAGYFAAVADAILRAQEEIFITDWWLSPEIYLKRPAH-SDDWRLDIMLKKKAEEGVHVS 416
Query: 374 ILIYKEVPIALKINSMYSKKRLLSIHENIKVLRSPDHMSTGIYYWSHHEKLVIIDHHICF 433
+L++KEV +AL INS YSKK L+ +H NIKV+R PD ++ W+HHEKL+++D + F
Sbjct: 417 VLLFKEVELALAINSGYSKKALMLLHPNIKVMRHPDQVT----LWAHHEKLLVVDQVVAF 476
Query: 434 IGGLDLCFGRYDTMEHKVSDF----------PPYT----------------WPGKDYYNP 493
+GGLDL +GR+D + ++++D PP + W GKDY N
Sbjct: 477 LGGLDLAYGRWDDLHYRLTDLGDSSESAAPQPPTSCSDLPATPDLTHNQLFWLGKDYSNL 536
Query: 494 RESEPNSWEDTMKDELEREKYPRMPWHDVHCALWGSPCRDVARHFVQRWNHAKRNKAPNE 553
+ + D ++RE PRMPW D+ + GSP RD+ARHF+QRWN K K +
Sbjct: 537 ITKDWVQLDRPFDDFIDRETMPRMPWRDIGVVVHGSPARDLARHFIQRWNFTKTTK--TK 596
Query: 554 EKIPLLMPQHHMVLPHYMGRSTELSFENNNSEQDHQKQTTVDFFSSLSPQQDIPLLLPQE 613
KIP + P+ + +ST S +P L
Sbjct: 597 YKIP--------IYPYLLPKST-------------------------STANQLPFTLS-- 656
Query: 614 VGGLPDSNIETNSLSMNHNFLNKPMEIQRSVMDSFRTYIVEPLTQYDAQTNGLLDEFGFL 673
GG + V+ L D + G L+
Sbjct: 657 -GG--------------------------------QCATVQVLRSVDRWSAGTLEN---- 716
Query: 674 DEFDALDHPREATIDAPAYMKTSDNWLETEHESNYVVAIDDVKEIGPLTASNCQIIRSVS 733
+ +L T ES + + I++ I +C R+V
Sbjct: 717 --------------------SILNAYLHTIRESQHFLYIENQFFI------SCSDGRTVL 776
Query: 734 QWSAGTSKPEASIHA---AYCFAIQEAKHFI-YIEGGLDDNGAATVRALMHWQYRSISWE 793
G + + A CF + + EG + G +++A++H+ YR++
Sbjct: 777 N-KVGDEIVDRILKAHKQGQCFRVYVLLPLLPGFEGDISTGGGNSIQAILHFTYRTLCRG 836
Query: 794 KTSILYHLNLLLGPKTQDYILFCGLRSYGRLFDGGPIATSQVYVHSKLMIIDDCITFIGS 853
+ SIL+ L +G + ++YI CGLR++G L GG + +Y+HSK++I DD IGS
Sbjct: 837 EYSILHRLKAAMGTEWRNYISVCGLRTHGEL--GGHPVSELIYIHSKMLIADDRTVIIGS 896
Query: 854 SNINDRSLLGSRDSEIGVIIEDKEFVDSSMNGKPWKAGKFAHSLRCSLWCEHLGLQLGEI 913
+NINDRSLLG RDSE+ V+IED E S MNG ++AG+FA SLR + LG
Sbjct: 897 ANINDRSLLGKRDSELAVLIEDTEMEPSLMNGVEYQAGRFALSLRKHCFSVILGAAARPH 924
Query: 914 SQISDPTAEATYKDLWVATAKENTAIYKDVFSCIPNDNINSRSSLRQHLADLRGKLDHNT 973
+ DP +A ++ LW TA+ N IY+ +F C+P++ S +LR++
Sbjct: 957 LDLRDPVCDAFFQ-LWQDTAESNANIYEQIFRCLPSNATRSLRALREY------------ 924
Query: 974 IDLGIAAEKIEIYENGEVKLIDPMER--LKCIRGHLVCFPLEFMRQEDLRPGFIESE 998
+ E + + P+ R L ++GHLV FPL+F+ E L P E
Sbjct: 1017 ----VVVEPL-------ATVSPPLARSELNQVQGHLVHFPLKFLEDEYLLPSLGSKE 924
BLAST of Sgr012749 vs. ExPASy Swiss-Prot
Match:
Q9Z280 (Phospholipase D1 OS=Mus musculus OX=10090 GN=Pld1 PE=1 SV=1)
HSP 1 Score: 352.4 bits (903), Expect = 1.9e-95
Identity = 291/1041 (27.95%), Postives = 469/1041 (45.05%), Query Frame = 0
Query: 44 ASIVSVSRPDTGDISPLLLSYTIQIQYKQFKWSLVKKASQVLYLH-FALKRRAFIE---- 103
A ++ V R + P + YTI++ + +F W + +K H LK +AFI
Sbjct: 83 AQVLEVERFTSTSRVPSINLYTIELTHGEFTWQVKRKFKHFQEFHRELLKYKAFIRIPIP 142
Query: 104 -ELHE-KQEQVKEWLQNLGIVDHTAVVHNDDESDVGAFPLHDEQTTRNRNVPSVAALPII 163
+ H +++ VKE + + + ++ +E G ++ T+ +P
Sbjct: 143 TKRHTFRRQNVKEEPREMPSLPRSSENAIQEEQFFGRRKQLEDYLTKILKMPMYRNYHAT 202
Query: 164 KPALGGQR--SISDKAKLAMQGYLNHFLGNLDI--VNS----------REVWAVLKPGFL 223
L + I D ++G + G I VN + W ++K FL
Sbjct: 203 TEFLDVSQLSFIHDLGPKGLEGMIMKRSGGHRIPGVNCCGHGRACYRWSKRWLIVKDSFL 262
Query: 224 ALVADPLDTKLLDIIVFDVLPTLEGKEGSQANLAYHVKDRNPLRYSFMVRCGNGNMKFRT 283
+ ++V G++ ++ + + L + +++C
Sbjct: 263 LYMKPDSGAIAFVLLVDKEFRVKVGRKETETKYGLRIDN---LSRTLILKC--------- 322
Query: 284 TSAAKVREWVTSINDAGLGPQDGWCHPHRFGSFAPQRGLSDDESRAQWFIDGRAAFEAIA 343
S R W +I + + HRFGS+A + + A+W+++ + FE IA
Sbjct: 323 NSYRHARWWGGAIEEFIRKHGADFLKDHRFGSYAALH----ENTLAKWYVNAKGYFEDIA 382
Query: 344 CSIEAAKSEIFITGWWLCPELYMQRPFHYHSSSRLDALLETKAKEGVKIYILIYKEVPIA 403
++E A EIFIT WWL PE++++RP + RLD +L+ KA++GV+I+I++YKEV +A
Sbjct: 383 NAMEEASEEIFITDWWLSPEIFLKRPVVEGNRWRLDCILKRKAQQGVRIFIMLYKEVELA 442
Query: 404 LKINSMYSKKRLLSIHENIKVLRSPDHMSTGIYYWSHHEKLVIIDHHICFIGGLDLCFGR 463
L INS YSK+ L+ +H NIKV+R PDH+S+ +Y W+HHEKLVIID + F+GG+DL +GR
Sbjct: 443 LGINSEYSKRTLMRLHPNIKVMRHPDHVSSSVYLWAHHEKLVIIDQSVAFVGGIDLAYGR 502
Query: 464 YDTMEHKVSDFPPY--TWPGKDYYNPRESEPNSWED-TMKDELEREKYPRMP--WHDVHC 523
+D EH+++D G + + S E ++KD+ E K + +
Sbjct: 503 WDDNEHRLTDVGSVKRVTSGLSLGSLTAASVESMESLSLKDKHEFHKKEPISKIVDETDM 562
Query: 524 ALWG-SPCRDVARHFVQRWNHAKRNKAPNEEKIPLLMP--------QHHMVLPHYMGRST 583
L G R ++ + R H R+ N + I + + H L H +
Sbjct: 563 KLKGIGKSRKFSKFSLYRQLH--RHHLHNADSISSIDSTSSYFSHCRSHQNLIHGLKPHL 622
Query: 584 ELSFENNNSEQDHQKQTTVDFFSSLSPQQDIPLLLPQEVGGLPDSNIETNSLSMNH--NF 643
+L ++ SEQ + +T D S S Q G+ + + ET NF
Sbjct: 623 KLFHPSSESEQGLTRHST-DTGSIRSVQT-----------GVGELHGETRFWHGKDYCNF 682
Query: 644 LNKP-MEIQRSVMDSFRTYIVEPLTQYD-------AQTNGLLDEFGFLDEFDALDHPREA 703
+ K +++ + D Y + +D + F F + P+
Sbjct: 683 VFKDWVQLDKPFADFIDRYSTPRMPWHDIGSVVHGKAARDVARHFIQRWNFTKIMKPKYR 742
Query: 704 TIDAPAYMKTSDNWLETEHESNYVVAIDDVKEIGPLTASNCQIIRSVSQWSAGTSKPEAS 763
++ P + S T HE Y ++ + Q++RS + WSAG E S
Sbjct: 743 SLSYPFLLPKSQ---ATAHELRY--------QVPGAVPAKVQLLRSAADWSAGIKHHEES 802
Query: 764 IHAAYCFAIQEAKHFIYI------------------------------------------ 823
IHAAY I+ +KH+IYI
Sbjct: 803 IHAAYIHVIENSKHYIYIENQFFISCADDKVVFNKVGDRIAQRILKAHREGQRYRVYIVI 862
Query: 824 ------EGGLDDNGAATVRALMHWQYRSISWEKTSILYHLNLLLGPKTQDYILFCGLRSY 883
EG + G ++A+MH+ YR++ ++SIL L LG K +YI FCGLR++
Sbjct: 863 PLLPGFEGDISTGGGNALQAIMHFNYRTMCRGESSILEQLKPELGNKWINYISFCGLRTH 922
Query: 884 GRLFDGGPIATSQVYVHSKLMIIDDCITFIGSSNINDRSLLGSRDSEIGVIIEDKEFVDS 943
L G + T +YVHSKL+I DD IGS+NINDRS+LG RDSE+ VI++D E V S
Sbjct: 923 AEL--EGNLVTELIYVHSKLLIADDNTVIIGSANINDRSMLGKRDSEMAVIVQDTETVPS 982
Query: 944 SMNGKPWKAGKFAHSLRCSLWCEHLGLQLGEISQISDPTAEATYKDLWVATAKENTAIYK 992
M+GK ++AG+FA LR + LG + DP ++ +K++WV+TA N IY
Sbjct: 983 VMDGKEYQAGRFARDLRLECFRLVLGYLSDPSEDLQDPVSDKFFKEIWVSTAARNATIYD 1042
BLAST of Sgr012749 vs. ExPASy Swiss-Prot
Match:
O14939 (Phospholipase D2 OS=Homo sapiens OX=9606 GN=PLD2 PE=1 SV=2)
HSP 1 Score: 352.1 bits (902), Expect = 2.5e-95
Identity = 253/831 (30.45%), Postives = 387/831 (46.57%), Query Frame = 0
Query: 194 WAVLKPGFLALVADPLDTKLLDII-VFDVLPTLEGKEGSQANLAYHVKDRNPLRYSFMVR 253
W V+K FL + L+T + + +FD P E + G ++ A H + S +++
Sbjct: 237 WLVVKDSFLLYMC--LETGAISFVQLFD--PGFEVQVGKRSTEARHGVRIDTSHRSLILK 296
Query: 254 CGNGNMKFRTTSAAKVREWVTSINDAGLGPQDGWCHPHRFGSFAPQRGLSDDESRAQWFI 313
C +S + R W I + GP + HR S+AP R + A+WF+
Sbjct: 297 C---------SSYRQARWWAQEITELAQGPGRDFLQLHRHDSYAPPR----PGTLARWFV 356
Query: 314 DGRAAFEAIACSIEAAKSEIFITGWWLCPELYMQRPFHYHSSSRLDALLETKAKEGVKIY 373
+G F A+A +I A+ EIFIT WWL PE+Y++RP H RLD +L+ KA+EGV++
Sbjct: 357 NGAGYFAAVADAILRAQEEIFITDWWLSPEVYLKRPAH-SDDWRLDIMLKRKAEEGVRVS 416
Query: 374 ILIYKEVPIALKINSMYSKKRLLSIHENIKVLRSPDHMSTGIYYWSHHEKLVIIDHHICF 433
IL++KEV +AL INS YSK+ L+ +H NIKV+R PD ++ W+HHEKL+++D + F
Sbjct: 417 ILLFKEVELALGINSGYSKRALMLLHPNIKVMRHPDQVT----LWAHHEKLLVVDQVVAF 476
Query: 434 IGGLDLCFGRYDTMEHKVSDF----------PP----------------YTWPGKDYYNP 493
+GGLDL +GR+D + ++++D PP + W GKDY N
Sbjct: 477 LGGLDLAYGRWDDLHYRLTDLGDSSESAASQPPTPRPDSPATPDLSHNQFFWLGKDYSNL 536
Query: 494 RESEPNSWEDTMKDELEREKYPRMPWHDVHCALWGSPCRDVARHFVQRWNHAKRNKAPNE 553
+ + +D ++RE PRMPW DV + G P RD+ARHF+QRWN K KA +
Sbjct: 537 ITKDWVQLDRPFEDFIDRETTPRMPWRDVGVVVHGLPARDLARHFIQRWNFTKTTKAKYK 596
Query: 554 EKIPLLMPQHHMVLPHYMGRSTELSFENNNSEQDHQKQTTVDFFSSLSPQQDIPLLLPQE 613
P + +LP S S +P LP
Sbjct: 597 ------TPTYPYLLP-----------------------------KSTSTANQLPFTLP-- 656
Query: 614 VGGLPDSNIETNSLSMNHNFLNKPMEIQRSVMDSFRTYIVEPLTQYDAQTNGLLDEFGFL 673
GG + V+ L D + G L+
Sbjct: 657 -GG--------------------------------QCTTVQVLRSVDRWSAGTLEN---- 716
Query: 674 DEFDALDHPREATIDAPAYMKTSDNWLETEHESNYVVAIDDVKEIGPLTASNCQIIRSVS 733
+ +L T ES + + I++ I +C R+V
Sbjct: 717 --------------------SILNAYLHTIRESQHFLYIENQFFI------SCSDGRTVL 776
Query: 734 QWSAGTSKPEASIHA---AYCFAIQEAKHFI-YIEGGLDDNGAATVRALMHWQYRSISWE 793
G + + A +C+ + + EG + G +++A++H+ YR++
Sbjct: 777 N-KVGDEIVDRILKAHKQGWCYRVYVLLPLLPGFEGDISTGGGNSIQAILHFTYRTLCRG 836
Query: 794 KTSILYHLNLLLGPKTQDYILFCGLRSYGRLFDGGPIATSQVYVHSKLMIIDDCITFIGS 853
+ SIL+ L +G +DYI CGLR++G L GG + +Y+HSK++I DD IGS
Sbjct: 837 EYSILHRLKAAMGTAWRDYISICGLRTHGEL--GGHPVSELIYIHSKVLIADDRTVIIGS 896
Query: 854 SNINDRSLLGSRDSEIGVIIEDKEFVDSSMNGKPWKAGKFAHSLRCSLWCEHLGLQLGEI 913
+NINDRSLLG RDSE+ V+IED E S MNG ++AG+FA SLR + LG
Sbjct: 897 ANINDRSLLGKRDSELAVLIEDTETEPSLMNGAEYQAGRFALSLRKHCFGVILGANTRPD 918
Query: 914 SQISDPTAEATYKDLWVATAKENTAIYKDVFSCIPNDNINSRSSLRQHLADLRGKLDHNT 973
+ DP + ++ LW A+ N IY+ +F C+P++ S +LR++
Sbjct: 957 LDLRDPICDDFFQ-LWQDMAESNANIYEQIFRCLPSNATRSLRTLREY------------ 918
Query: 974 IDLGIAAEKIEIYENGEVKLIDPMER--LKCIRGHLVCFPLEFMRQEDLRP 992
+A E + + P+ R L ++GHLV FPL+F+ E L P
Sbjct: 1017 ----VAVEPL-------ATVSPPLARSELTQVQGHLVHFPLKFLEDESLLP 918
BLAST of Sgr012749 vs. ExPASy TrEMBL
Match:
A0A6J1DUS1 (Phospholipase OS=Momordica charantia OX=3673 GN=LOC111023278 PE=3 SV=1)
HSP 1 Score: 1818.9 bits (4710), Expect = 0.0e+00
Identity = 908/1110 (81.80%), Postives = 950/1110 (85.59%), Query Frame = 0
Query: 1 MSTERLIPSGTARSE-AEPPRLVSASHSFRQCGEPAWIFEELPVASIVSVSRPDTGDISP 60
MSTERLIP+GTA SE AEPPRLV+ASHSFRQCGEPA +FEELPVASIVSVSRPDTGDISP
Sbjct: 1 MSTERLIPNGTAPSEAAEPPRLVAASHSFRQCGEPARVFEELPVASIVSVSRPDTGDISP 60
Query: 61 LLLSYTIQIQYKQFKWSLVKKASQVLYLHFALKRRAFIEELHEKQEQVKEWLQNLGIVDH 120
LLLSYTIQIQYKQFKW+LVKKASQV+YLHFALKRR FIEELHEKQEQVKEWLQNLGI DH
Sbjct: 61 LLLSYTIQIQYKQFKWNLVKKASQVIYLHFALKRRTFIEELHEKQEQVKEWLQNLGIFDH 120
Query: 121 TAVVHNDDESDVGAFPLHDEQTTRNRNVPSVAALPIIKPALGGQRSISDKAKLAMQGYLN 180
TAVVHNDDESDV A DEQTTRNRNVPSVAALPIIKPALGGQRSISDKAKLAMQGYLN
Sbjct: 121 TAVVHNDDESDVAA----DEQTTRNRNVPSVAALPIIKPALGGQRSISDKAKLAMQGYLN 180
Query: 181 HFLGNLDIVNSRE----------------------------------------------- 240
HFLGNLDIVNSRE
Sbjct: 181 HFLGNLDIVNSREVCKFLEVSKMSFVREYGPKLKEGYLMVKHLKQLTGSDSSIKCCACHW 240
Query: 241 ----------VWAVLKPGFLALVADPLDTKLLDIIVFDVLPTLEGKEGSQANLAYHVKDR 300
VWAVLKPGFLALVA PLDTKLLDI+VFDVLP+LE KE SQA LA H+K+R
Sbjct: 241 CSCCDYNWKKVWAVLKPGFLALVAHPLDTKLLDIVVFDVLPSLEEKEESQAYLASHIKER 300
Query: 301 NPLRYSFMVRCGNGNMKFRTTSAAKVREWVTSINDAGLGPQDGWCHPHRFGSFAPQRGLS 360
NPLRYSF VRCGNGNMKFRTTS+AKVREWV+SINDAG GPQDGWCHPHRFGSFAPQRGLS
Sbjct: 301 NPLRYSFKVRCGNGNMKFRTTSSAKVREWVSSINDAGFGPQDGWCHPHRFGSFAPQRGLS 360
Query: 361 DDESRAQWFIDGRAAFEAIACSIEAAKSEIFITGWWLCPELYMQRPFHYHSSSRLDALLE 420
DDES+AQWFIDG AAFEAIACSIEAAKSEIFITGWWLCPELYM+RPF YH SSRLDALLE
Sbjct: 361 DDESQAQWFIDGGAAFEAIACSIEAAKSEIFITGWWLCPELYMRRPFQYHYSSRLDALLE 420
Query: 421 TKAKEGVKIYILIYKEVPIALKINSMYSKKRLLSIHENIKVLRSPDHMSTGIYYWSHHEK 480
TKAKEGVKIYIL+YKEVPIALKINSMYSK+RLL+IHENIKVLRSPDHMSTGIYYWSHHEK
Sbjct: 421 TKAKEGVKIYILMYKEVPIALKINSMYSKRRLLNIHENIKVLRSPDHMSTGIYYWSHHEK 480
Query: 481 LVIIDHHICFIGGLDLCFGRYDTMEHKVSDFPPYTWPGKDYYNPRESEPNSWEDTMKDEL 540
LVI+DHHICFIGGLDLCFGRYDTMEHKVSDFPPYTWPGKDYYNPRESEPNSWEDTMKDEL
Sbjct: 481 LVIVDHHICFIGGLDLCFGRYDTMEHKVSDFPPYTWPGKDYYNPRESEPNSWEDTMKDEL 540
Query: 541 EREKYPRMPWHDVHCALWGSPCRDVARHFVQRWNHAKRNKAPNEEKIPLLMPQHHMVLPH 600
+REKYPRMPWHDVHCALWGSPCRD+ARHFVQRWNHAKRNKAPNEEKIPLLMPQHHMVLPH
Sbjct: 541 DREKYPRMPWHDVHCALWGSPCRDIARHFVQRWNHAKRNKAPNEEKIPLLMPQHHMVLPH 600
Query: 601 YMGRSTELSFENNNSEQDHQKQTTVDFFSSLSPQQDIPLLLPQEVGGLPDSNIETNSLSM 660
YMGRSTELSFEN +SEQDHQKQTTVDF+SSLSPQQDIPLLLPQE GGLPD NIET+S SM
Sbjct: 601 YMGRSTELSFENKDSEQDHQKQTTVDFYSSLSPQQDIPLLLPQEAGGLPDLNIETSSPSM 660
Query: 661 NHNFLNKPMEIQRSVMDSFRTYIVEPLTQYDAQTNGLLDEFGFLDEFDALDHPREATIDA 720
NH+FLNKP+EIQR +MDS LTQY A+T G LDEFGFLDEF L HPREA IDA
Sbjct: 661 NHHFLNKPVEIQRRLMDS--------LTQY-AETYGSLDEFGFLDEFGDLGHPREAAIDA 720
Query: 721 PAYMKTSDNWLETEHESNYVVAIDDVKEIGPLTASNCQIIRSVSQWSAGTSKPEASIHAA 780
PAYMKTSD+WLETE ESN+VVA++DVKEIGP+T SNCQ+IRSVSQWSAGTSKPEASIH A
Sbjct: 721 PAYMKTSDDWLETEPESNHVVAVNDVKEIGPITTSNCQVIRSVSQWSAGTSKPEASIHGA 780
Query: 781 YCFAIQEAKHFIYIE--------------------------------------------- 840
YCFAIQEAKHFIYIE
Sbjct: 781 YCFAIQEAKHFIYIENQFFISGLSGDETIQNRVLEALYQRILLADKEQQCFRVIVVLPLL 840
Query: 841 ----GGLDDNGAATVRALMHWQYRSISWEKTSILYHLNLLLGPKTQDYILFCGLRSYGRL 900
GG+DDNGAATVRALMHWQYR+ISWEKTSILY LNLLLGPKTQDYILFCGLRSYGRL
Sbjct: 841 PGFQGGVDDNGAATVRALMHWQYRTISWEKTSILYRLNLLLGPKTQDYILFCGLRSYGRL 900
Query: 901 FDGGPIATSQVYVHSKLMIIDDCITFIGSSNINDRSLLGSRDSEIGVIIEDKEFVDSSMN 960
FDGGPIATSQVYVHSKLMIIDDCITFIGSSNINDRSLLGSRDSEIGV IEDKEFVDSSMN
Sbjct: 901 FDGGPIATSQVYVHSKLMIIDDCITFIGSSNINDRSLLGSRDSEIGVFIEDKEFVDSSMN 960
Query: 961 GKPWKAGKFAHSLRCSLWCEHLGLQLGEISQISDPTAEATYKDLWVATAKENTAIYKDVF 1004
GKPWKAGKFAHSLRCSLWCEHLGL+L E+SQISDP AEATYKD+W+ATAKENTAIY+DVF
Sbjct: 961 GKPWKAGKFAHSLRCSLWCEHLGLRLEEVSQISDPIAEATYKDIWLATAKENTAIYEDVF 1020
BLAST of Sgr012749 vs. ExPASy TrEMBL
Match:
A0A6J1DQ37 (Phospholipase OS=Momordica charantia OX=3673 GN=LOC111023278 PE=3 SV=1)
HSP 1 Score: 1806.2 bits (4677), Expect = 0.0e+00
Identity = 905/1120 (80.80%), Postives = 949/1120 (84.73%), Query Frame = 0
Query: 1 MSTERLIPSGTARSE-AEPPRLVSASHSFRQCGEPAWIFEELPVASIVSVSRPDTGDISP 60
MSTERLIP+GTA SE AEPPRLV+ASHSFRQCGEPA +FEELPVASIVSVSRPDTGDISP
Sbjct: 1 MSTERLIPNGTAPSEAAEPPRLVAASHSFRQCGEPARVFEELPVASIVSVSRPDTGDISP 60
Query: 61 LLLSYTIQIQYKQFKWSLVKKASQVLYLHFALKRRAFIEELHEKQEQVKEWLQNLGIVDH 120
LLLSYTIQIQYKQFKW+LVKKASQV+YLHFALKRR FIEELHEKQEQVKEWLQNLGI DH
Sbjct: 61 LLLSYTIQIQYKQFKWNLVKKASQVIYLHFALKRRTFIEELHEKQEQVKEWLQNLGIFDH 120
Query: 121 TAVVHNDDESDVGAFPLHDEQTTRNRNVPSVAALPIIKPALGGQRSISDKAKLAMQGYLN 180
TAVVHNDDESDV A DEQTTRNRNVPSVAALPIIKPALGGQRSISDKAKLAMQGYLN
Sbjct: 121 TAVVHNDDESDVAA----DEQTTRNRNVPSVAALPIIKPALGGQRSISDKAKLAMQGYLN 180
Query: 181 HFLGNLDIVNSRE----------------------------------------------- 240
HFLGNLDIVNSRE
Sbjct: 181 HFLGNLDIVNSREVCKFLEVSKMSFVREYGPKLKEGYLMVKHLKQLTGSDSSIKCCACHW 240
Query: 241 ----------VWAVLKPGFLALVADPLDTKLLDIIVFDVLPTLEGKEGSQANLAYHVKDR 300
VWAVLKPGFLALVA PLDTKLLDI+VFDVLP+LE KE SQA LA H+K+R
Sbjct: 241 CSCCDYNWKKVWAVLKPGFLALVAHPLDTKLLDIVVFDVLPSLEEKEESQAYLASHIKER 300
Query: 301 NPLRYSFMVRCGNGNMKFRTTSAAKVREWVTSINDAGLGPQDGWCHPHRFGSFAPQRGLS 360
NPLRYSF VRCGNGNMKFRTTS+AKVREWV+SINDAG GPQDGWCHPHRFGSFAPQRGLS
Sbjct: 301 NPLRYSFKVRCGNGNMKFRTTSSAKVREWVSSINDAGFGPQDGWCHPHRFGSFAPQRGLS 360
Query: 361 DDESRAQWFIDGRAAFEAIACSIEAAKSEIFITGWWLCPELYMQRPFHYHSSSRLDALLE 420
DDES+AQWFIDG AAFEAIACSIEAAKSEIFITGWWLCPELYM+RPF YH SSRLDALLE
Sbjct: 361 DDESQAQWFIDGGAAFEAIACSIEAAKSEIFITGWWLCPELYMRRPFQYHYSSRLDALLE 420
Query: 421 TKAKEGVKIYILIYKEVPIALKINSMYSKKRLLSIHENIKVLRSPDHMSTGIYYWSHHEK 480
TKAKEGVKIYIL+YKEVPIALKINSMYSK+RLL+IHENIKVLRSPDHMSTGIYYWSHHEK
Sbjct: 421 TKAKEGVKIYILMYKEVPIALKINSMYSKRRLLNIHENIKVLRSPDHMSTGIYYWSHHEK 480
Query: 481 LVIIDHHICFIGGLDLCFGRYDTMEHKVSDFPPYTWPGKDYYNPRESEPNSWEDTMKDEL 540
LVI+DHHICFIGGLDLCFGRYDTMEHKVSDFPPYTWPGKDYYNPRESEPNSWEDTMKDEL
Sbjct: 481 LVIVDHHICFIGGLDLCFGRYDTMEHKVSDFPPYTWPGKDYYNPRESEPNSWEDTMKDEL 540
Query: 541 EREKYPRMPWHDVHCALWGSPCRDVARHFVQRWNHAKRNKAPNEEKIPLLMPQHHMVLPH 600
+REKYPRMPWHDVHCALWGSPCRD+ARHFVQRWNHAKRNKAPNEEKIPLLMPQHHMVLPH
Sbjct: 541 DREKYPRMPWHDVHCALWGSPCRDIARHFVQRWNHAKRNKAPNEEKIPLLMPQHHMVLPH 600
Query: 601 YMGRSTELSFENNNSEQDHQKQTTVDFFSSLSPQQDIPLLLPQEVGGLPDSNIETNSLSM 660
YMGRSTELSFEN +SEQDHQKQTTVDF+SSLSPQQDIPLLLPQE GGLPD NIET+S SM
Sbjct: 601 YMGRSTELSFENKDSEQDHQKQTTVDFYSSLSPQQDIPLLLPQEAGGLPDLNIETSSPSM 660
Query: 661 NHNFLNKPMEIQRSVMDSFRTYIVEPLTQYDAQTNGLLDEFGFLDEFDALDHPREATIDA 720
NH+FLNKP+EIQR +MDS LTQY A+T G LDEFGFLDEF L HPREA IDA
Sbjct: 661 NHHFLNKPVEIQRRLMDS--------LTQY-AETYGSLDEFGFLDEFGDLGHPREAAIDA 720
Query: 721 PAYMKTSDNWLETEHESNYVVAIDDVKEIGPLTASNCQIIRSVSQWSAGTSKPEASIHAA 780
PAYMKTSD+WLETE ESN+VVA++DVKEIGP+T SNCQ+IRSVSQWSAGTSKPEASIH A
Sbjct: 721 PAYMKTSDDWLETEPESNHVVAVNDVKEIGPITTSNCQVIRSVSQWSAGTSKPEASIHGA 780
Query: 781 YCFAIQEAKHFIYIE--------------------------------------------- 840
YCFAIQEAKHFIYIE
Sbjct: 781 YCFAIQEAKHFIYIENQFFISGLSGDETIQNRVLEALYQRILLADKEQQCFRVIVVLPLL 840
Query: 841 ----GGLDDNGAATVRALMHWQYRSISWEKTSILYHLNLLLGPKTQDYILFCGLRSYGRL 900
GG+DDNGAATVRALMHWQYR+ISWEKTSILY LNLLLGPKTQDYILFCGLRSYGRL
Sbjct: 841 PGFQGGVDDNGAATVRALMHWQYRTISWEKTSILYRLNLLLGPKTQDYILFCGLRSYGRL 900
Query: 901 FDGGPIATSQVYVHSKLMIIDDCITFIGSSNINDRSLLGSRDSEIGVIIEDKEFVDSSMN 960
FDGGPIATSQVYVHSKLMIIDDCITFIGSSNINDRSLLGSRDSEIGV IEDKEFVDSSMN
Sbjct: 901 FDGGPIATSQVYVHSKLMIIDDCITFIGSSNINDRSLLGSRDSEIGVFIEDKEFVDSSMN 960
Query: 961 GKPWKAGKFAHSLRCSLWCEHLGLQLGEISQISDPTAEATYKDLWVATAKENTAIYKDVF 1014
GKPWKAGKFAHSLRCSLWCEHLGL+L E+SQISDP AEATYKD+W+ATAKENTAIY+DVF
Sbjct: 961 GKPWKAGKFAHSLRCSLWCEHLGLRLEEVSQISDPIAEATYKDIWLATAKENTAIYEDVF 1020
BLAST of Sgr012749 vs. ExPASy TrEMBL
Match:
A0A6J1G515 (Phospholipase OS=Cucurbita moschata OX=3662 GN=LOC111450778 PE=3 SV=1)
HSP 1 Score: 1782.3 bits (4615), Expect = 0.0e+00
Identity = 883/1109 (79.62%), Postives = 934/1109 (84.22%), Query Frame = 0
Query: 1 MSTERLIPSGTARSEAEPPRLVSASHSFRQCGEPAWIFEELPVASIVSVSRPDTGDISPL 60
MSTERLI SGT SEAE PRL S+SHSFRQCGEPA +FEELP+ASIVSVSRPDTGDISPL
Sbjct: 1 MSTERLISSGTTPSEAEAPRLASSSHSFRQCGEPARVFEELPIASIVSVSRPDTGDISPL 60
Query: 61 LLSYTIQIQYKQFKWSLVKKASQVLYLHFALKRRAFIEELHEKQEQVKEWLQNLGIVDHT 120
LLSYTI+IQYKQFKWSLVKKASQVLYLHFALKRRAFIEELHEKQEQVKEWL NLGIVDHT
Sbjct: 61 LLSYTIEIQYKQFKWSLVKKASQVLYLHFALKRRAFIEELHEKQEQVKEWLHNLGIVDHT 120
Query: 121 AVVHNDDESDVGAFPLHDEQTTRNRNVPSVAALPIIKPALGGQRSISDKAKLAMQGYLNH 180
A VH+DDESD G FPLHDEQTT+NRNVPSVAALPIIKPA+GGQRSISDKAKLAMQGYLNH
Sbjct: 121 AAVHHDDESDDGLFPLHDEQTTKNRNVPSVAALPIIKPAMGGQRSISDKAKLAMQGYLNH 180
Query: 181 FLGNLDIVNSRE------------------------------------------------ 240
F GNLDIVN+RE
Sbjct: 181 FFGNLDIVNTREVCKFLEVSKMSFVREHGPKLKEGYLMVKHLKQLTGSDSSIKCFACHWC 240
Query: 241 ---------VWAVLKPGFLALVADPLDTKLLDIIVFDVLPTLEGKEGSQANLAYHVKDRN 300
VWAVLKPGFLALVADP+DTKLLDIIVFDVL TLE KEGSQA LAYHVK+RN
Sbjct: 241 SCCVYNWKKVWAVLKPGFLALVADPMDTKLLDIIVFDVLQTLEEKEGSQACLAYHVKERN 300
Query: 301 PLRYSFMVRCGNGNMKFRTTSAAKVREWVTSINDAGLGPQDGWCHPHRFGSFAPQRGLSD 360
PLRYSF VR GNGN++FRTTS AKV++WV++INDAG G +DGWCHPHRFGSFAPQRGLSD
Sbjct: 301 PLRYSFKVRGGNGNLRFRTTSTAKVKKWVSAINDAGFGSKDGWCHPHRFGSFAPQRGLSD 360
Query: 361 DESRAQWFIDGRAAFEAIACSIEAAKSEIFITGWWLCPELYMQRPFHYHSSSRLDALLET 420
DES+AQWFIDGRAAFEAIACSIEAAKSEIFITGWWLCPELYM+RPFH HSSSRLDALLET
Sbjct: 361 DESQAQWFIDGRAAFEAIACSIEAAKSEIFITGWWLCPELYMRRPFHNHSSSRLDALLET 420
Query: 421 KAKEGVKIYILIYKEVPIALKINSMYSKKRLLSIHENIKVLRSPDHMSTGIYYWSHHEKL 480
KAKEGVKIYIL+YKEVPIALKINS YSKKRLL+IHENIKVLRSPDHMSTGIYYWSHHEKL
Sbjct: 421 KAKEGVKIYILMYKEVPIALKINSRYSKKRLLNIHENIKVLRSPDHMSTGIYYWSHHEKL 480
Query: 481 VIIDHHICFIGGLDLCFGRYDTMEHKVSDFPPYTWPGKDYYNPRESEPNSWEDTMKDELE 540
VI+DHHICFIGGLDLCFGRYDTMEHKVSDFPPYTWPGKDYYNPRESEPNSWEDTM DELE
Sbjct: 481 VIVDHHICFIGGLDLCFGRYDTMEHKVSDFPPYTWPGKDYYNPRESEPNSWEDTMSDELE 540
Query: 541 REKYPRMPWHDVHCALWGSPCRDVARHFVQRWNHAKRNKAPNEEKIPLLMPQHHMVLPHY 600
REK PRMPWHDVHCALWGSPCRD+ARHFVQRWNHAKR+KAPNEEKIPLLMPQH MVLPHY
Sbjct: 541 REKCPRMPWHDVHCALWGSPCRDIARHFVQRWNHAKRDKAPNEEKIPLLMPQHDMVLPHY 600
Query: 601 MGRSTELSFENNNSEQDHQKQTTVDFFSSLSPQQDIPLLLPQEVGGLPDSNIETNSLSMN 660
MGRSTELSF+N SEQDHQKQ T DFF S SPQ+DIPLL+PQEVGGLPDSN+E NS
Sbjct: 601 MGRSTELSFKNEGSEQDHQKQNTEDFFFSSSPQEDIPLLMPQEVGGLPDSNMERNSQ--- 660
Query: 661 HNFLNKPMEIQRSVMDSFRTYIVEPLTQYDAQTNGLLDEFGFLDEFDALDHPREATIDAP 720
+NK MEIQ S MDSF+ Y VE LTQY+ Q NGL DEFGFLDEF REAT D P
Sbjct: 661 ---MNKQMEIQSSAMDSFQAYNVESLTQYE-QANGLFDEFGFLDEFGDFGLSREATTDTP 720
Query: 721 AYMKTSDNWLETEHESNYVVAIDDVKEIGPLTASNCQIIRSVSQWSAGTSKPEASIHAAY 780
YMKTSD+WLETEHESN+VVA+++VKEIGPLT +NCQ+IRSVSQWSAGTSKPEASIHAAY
Sbjct: 721 PYMKTSDDWLETEHESNHVVAVNEVKEIGPLTTTNCQVIRSVSQWSAGTSKPEASIHAAY 780
Query: 781 CFAIQEAKHFIYIE---------------------------------------------- 840
C AIQEAKHFIYIE
Sbjct: 781 CSAIQEAKHFIYIENQFFISGLSGDETIHNRVLEALYQRIWLAHKEKQCFRVIIVLPLLP 840
Query: 841 ---GGLDDNGAATVRALMHWQYRSISWEKTSILYHLNLLLGPKTQDYILFCGLRSYGRLF 900
GG+DDNGAATVRALMHWQYR+ISWEKTSILY LNLLLG KTQDYILFCGLRSYGRLF
Sbjct: 841 GFQGGVDDNGAATVRALMHWQYRTISWEKTSILYRLNLLLGSKTQDYILFCGLRSYGRLF 900
Query: 901 DGGPIATSQVYVHSKLMIIDDCITFIGSSNINDRSLLGSRDSEIGVIIEDKEFVDSSMNG 960
DGGPIATSQVYVHSKLMIIDDCITFIGSSNINDRSLLGSRDSEIGVIIEDKEFVDS MNG
Sbjct: 901 DGGPIATSQVYVHSKLMIIDDCITFIGSSNINDRSLLGSRDSEIGVIIEDKEFVDSYMNG 960
Query: 961 KPWKAGKFAHSLRCSLWCEHLGLQLGEISQISDPTAEATYKDLWVATAKENTAIYKDVFS 1004
KPWKAGKFAHSLRCSLWCEHLGL LGE+SQI DP EATYKDLW+ATAKENTAIY+DVFS
Sbjct: 961 KPWKAGKFAHSLRCSLWCEHLGLHLGEVSQILDPIVEATYKDLWIATAKENTAIYEDVFS 1020
BLAST of Sgr012749 vs. ExPASy TrEMBL
Match:
A0A6J1KFT8 (Phospholipase OS=Cucurbita maxima OX=3661 GN=LOC111493911 PE=3 SV=1)
HSP 1 Score: 1778.8 bits (4606), Expect = 0.0e+00
Identity = 882/1109 (79.53%), Postives = 932/1109 (84.04%), Query Frame = 0
Query: 1 MSTERLIPSGTARSEAEPPRLVSASHSFRQCGEPAWIFEELPVASIVSVSRPDTGDISPL 60
MSTERLI SGT SEAEPPRL S+SHSFRQCGE A +FEELP+ASIVSVSRPDTGDISPL
Sbjct: 1 MSTERLISSGTTPSEAEPPRLASSSHSFRQCGESARVFEELPIASIVSVSRPDTGDISPL 60
Query: 61 LLSYTIQIQYKQFKWSLVKKASQVLYLHFALKRRAFIEELHEKQEQVKEWLQNLGIVDHT 120
LLSYTI+IQYKQF WSLVKKASQVLYLHFALKRRAFIEELHEKQEQVKEWL NLGIVDHT
Sbjct: 61 LLSYTIEIQYKQFIWSLVKKASQVLYLHFALKRRAFIEELHEKQEQVKEWLHNLGIVDHT 120
Query: 121 AVVHNDDESDVGAFPLHDEQTTRNRNVPSVAALPIIKPALGGQRSISDKAKLAMQGYLNH 180
A VH+DDESD G FPLHDEQTT+NRNVPSVAALPIIKPA+GGQRSISDKAKLAMQGYLNH
Sbjct: 121 AAVHHDDESDDGLFPLHDEQTTKNRNVPSVAALPIIKPAMGGQRSISDKAKLAMQGYLNH 180
Query: 181 FLGNLDIVNSRE------------------------------------------------ 240
F GNLDIVN+RE
Sbjct: 181 FFGNLDIVNTREVCKFLEVSKMSFVREHGPKLKEGYLMVKHLKQLTGSDSSIKCFACHWC 240
Query: 241 ---------VWAVLKPGFLALVADPLDTKLLDIIVFDVLPTLEGKEGSQANLAYHVKDRN 300
VWAVLKPGFLALVADP+DTKLLDIIVFDVLPTLE KEGSQA LAYHVK+RN
Sbjct: 241 SCCVYNWKKVWAVLKPGFLALVADPMDTKLLDIIVFDVLPTLEEKEGSQACLAYHVKERN 300
Query: 301 PLRYSFMVRCGNGNMKFRTTSAAKVREWVTSINDAGLGPQDGWCHPHRFGSFAPQRGLSD 360
PLRYSF VR GNGNM+FRTTS AKV++WV++INDAG G +DGWCHPHRFGSFAPQRGLSD
Sbjct: 301 PLRYSFKVRGGNGNMRFRTTSTAKVKKWVSAINDAGFGSKDGWCHPHRFGSFAPQRGLSD 360
Query: 361 DESRAQWFIDGRAAFEAIACSIEAAKSEIFITGWWLCPELYMQRPFHYHSSSRLDALLET 420
DES+AQWFIDGRAAFEAIACSIEAAKSEIFITGWWLCPELY++RPFH HSSSRLDALLET
Sbjct: 361 DESQAQWFIDGRAAFEAIACSIEAAKSEIFITGWWLCPELYLRRPFHNHSSSRLDALLET 420
Query: 421 KAKEGVKIYILIYKEVPIALKINSMYSKKRLLSIHENIKVLRSPDHMSTGIYYWSHHEKL 480
KAKEGVKIYIL+YKEVPIALKINS YSKKRLL+IHENIKVLRSPDHMSTGIYYWSHHEKL
Sbjct: 421 KAKEGVKIYILMYKEVPIALKINSRYSKKRLLNIHENIKVLRSPDHMSTGIYYWSHHEKL 480
Query: 481 VIIDHHICFIGGLDLCFGRYDTMEHKVSDFPPYTWPGKDYYNPRESEPNSWEDTMKDELE 540
VI+DHHICFIGGLDLCFGRYDTMEHKVSDFPPYTWPGKDYYNPRESEPNSWEDTM DELE
Sbjct: 481 VIVDHHICFIGGLDLCFGRYDTMEHKVSDFPPYTWPGKDYYNPRESEPNSWEDTMSDELE 540
Query: 541 REKYPRMPWHDVHCALWGSPCRDVARHFVQRWNHAKRNKAPNEEKIPLLMPQHHMVLPHY 600
REK PRMPWHDVHCALWGSPCRD+ARHFVQRWNHAKR+KAPNEEKIPLLMPQH MVLPHY
Sbjct: 541 REKCPRMPWHDVHCALWGSPCRDIARHFVQRWNHAKRDKAPNEEKIPLLMPQHDMVLPHY 600
Query: 601 MGRSTELSFENNNSEQDHQKQTTVDFFSSLSPQQDIPLLLPQEVGGLPDSNIETNSLSMN 660
MGRSTELSF+N SEQDHQKQ T DFF S SPQ+DIPLL+PQEVGGLP SN+E NS
Sbjct: 601 MGRSTELSFKNKGSEQDHQKQNTEDFFFSSSPQEDIPLLMPQEVGGLPVSNMERNSQ--- 660
Query: 661 HNFLNKPMEIQRSVMDSFRTYIVEPLTQYDAQTNGLLDEFGFLDEFDALDHPREATIDAP 720
+NK MEIQ S MDSF+ Y VE LTQY+ Q NGL DEFGFLDEF REAT D P
Sbjct: 661 ---MNKQMEIQSSAMDSFQAYNVESLTQYE-QANGLFDEFGFLDEFGDFGLSREATTDTP 720
Query: 721 AYMKTSDNWLETEHESNYVVAIDDVKEIGPLTASNCQIIRSVSQWSAGTSKPEASIHAAY 780
YMKTSD+WLETEHESN+VVA+++VKEIGPLT +NCQ+IRSVSQWSAGTSKPEASIHAAY
Sbjct: 721 TYMKTSDDWLETEHESNHVVAVNEVKEIGPLTTTNCQVIRSVSQWSAGTSKPEASIHAAY 780
Query: 781 CFAIQEAKHFIYIE---------------------------------------------- 840
C AIQEAKHFIYIE
Sbjct: 781 CSAIQEAKHFIYIENQFFISGLSGDETIHNRVLETLYQRIWLAHKEKQCFRVIIVLPLLP 840
Query: 841 ---GGLDDNGAATVRALMHWQYRSISWEKTSILYHLNLLLGPKTQDYILFCGLRSYGRLF 900
GG+DDNGAATVRALMHWQYR+ISWEKTSILY LNLLLG KTQDYILFCGLRSYGRLF
Sbjct: 841 GFQGGVDDNGAATVRALMHWQYRTISWEKTSILYRLNLLLGSKTQDYILFCGLRSYGRLF 900
Query: 901 DGGPIATSQVYVHSKLMIIDDCITFIGSSNINDRSLLGSRDSEIGVIIEDKEFVDSSMNG 960
DGGPIATSQVYVHSKLMIIDDCITFIGSSNINDRSLLGSRDSEIGVIIEDKEFVDS MNG
Sbjct: 901 DGGPIATSQVYVHSKLMIIDDCITFIGSSNINDRSLLGSRDSEIGVIIEDKEFVDSCMNG 960
Query: 961 KPWKAGKFAHSLRCSLWCEHLGLQLGEISQISDPTAEATYKDLWVATAKENTAIYKDVFS 1004
KPWKAGKFAHSLRCSLWCEHLGL LGE+SQI DP EATYKDLW+ATAKENTAIY+DVFS
Sbjct: 961 KPWKAGKFAHSLRCSLWCEHLGLHLGEVSQILDPIVEATYKDLWIATAKENTAIYEDVFS 1020
BLAST of Sgr012749 vs. ExPASy TrEMBL
Match:
A0A5D3BB20 (Phospholipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G001390 PE=3 SV=1)
HSP 1 Score: 1761.9 bits (4562), Expect = 0.0e+00
Identity = 871/1067 (81.63%), Postives = 928/1067 (86.97%), Query Frame = 0
Query: 1 MSTERLIPSGTARSEAEPPRL-VSASHSFRQCGEPAWIFEELPVASIVSVSRPDTGDISP 60
MST+RLI SGT SE EPPRL SA HSFRQC E A +FEELP+ SIVSVSRPDTGDISP
Sbjct: 1 MSTKRLISSGTTPSETEPPRLFASALHSFRQCVESARVFEELPIVSIVSVSRPDTGDISP 60
Query: 61 LLLSYTIQIQYKQFKWSLVKKASQVLYLHFALKRRAFIEELHEKQEQVKEWLQNLGIVDH 120
LLLSYTI+IQYKQFKW LVKKASQVLYLHF+LKRRAFIEELHEKQEQVKEWL N+GIVDH
Sbjct: 61 LLLSYTIEIQYKQFKWRLVKKASQVLYLHFSLKRRAFIEELHEKQEQVKEWLHNMGIVDH 120
Query: 121 TAVVHNDDESDVGAFPLHDEQTTRNRNVPSVAALPIIKPALGGQRSISDKAKLAMQGYLN 180
AVVH+DDESD GAF LHDEQTTRNRNVPSVAALPIIKPALGGQRSISDKAKLAMQGYLN
Sbjct: 121 PAVVHHDDESDDGAFSLHDEQTTRNRNVPSVAALPIIKPALGGQRSISDKAKLAMQGYLN 180
Query: 181 HFLGNLDIVNSREVWAVLKPGFLALVADPLDTKLLDIIVFDVLPTLEGKEGSQANLAYHV 240
HF GNLDIVNSREVWAVLKPGFLALVADP+D+KLLDIIVFDVLPT+E +EGSQA LAYHV
Sbjct: 181 HFFGNLDIVNSREVWAVLKPGFLALVADPMDSKLLDIIVFDVLPTVE-EEGSQACLAYHV 240
Query: 241 KDRNPLRYSFMVRCGNGNMKFRTTSAAKVREWVTSINDAGLGPQDGWCHPHRFGSFAPQR 300
K+RNPL+ VR G+G+++FRTTS AKVREWV+SINDAG G +DGWCHPHRFGSFAPQR
Sbjct: 241 KERNPLQCFEKVRRGDGDIRFRTTSTAKVREWVSSINDAGFGAKDGWCHPHRFGSFAPQR 300
Query: 301 GLSDDESRAQWFIDGRAAFEAIACSIEAAKSEIFITGWWLCPELYMQRPFHYHSSSRLDA 360
GLSDDES+AQWFIDGRAAFEAIACSIEAAKSEIFITGWWLCPELY +RPFH HSSSRLDA
Sbjct: 301 GLSDDESQAQWFIDGRAAFEAIACSIEAAKSEIFITGWWLCPELYTRRPFHNHSSSRLDA 360
Query: 361 LLETKAKEGVKIYILIYKEVPIALKINSMYSKKRLLSIHENIKVLRSPDHMSTGIYYWSH 420
LLETKAKEGVKIYIL+YKEVPIALKINSMYSKKRLL+IHENIKVLRSPDHMSTGIYYWSH
Sbjct: 361 LLETKAKEGVKIYILMYKEVPIALKINSMYSKKRLLNIHENIKVLRSPDHMSTGIYYWSH 420
Query: 421 HEKLVIIDHHICFIGGLDLCFGRYDTMEHKVSDFPPYTWPGKDYYNP------------- 480
HEK+V++DH ICFIGGLDLCFGRYDTMEHKVSDFPPYTWPGKDYYNP
Sbjct: 421 HEKIVVVDHQICFIGGLDLCFGRYDTMEHKVSDFPPYTWPGKDYYNPRILVNIIDLPNLR 480
Query: 481 --RESEPNSWEDTMKDELEREKYPRMPWHDVHCALWGSPCRDVARHFVQRWNHAKRNKAP 540
RESEPNSWEDTMKDELEREK PRMPWHDVHCALWGSPCRDVARHFVQRWNHAKRNKAP
Sbjct: 481 VFRESEPNSWEDTMKDELEREKCPRMPWHDVHCALWGSPCRDVARHFVQRWNHAKRNKAP 540
Query: 541 NEEKIPLLMPQHHMVLPHYMGRSTELSFENNNSEQDHQKQTTVDFFSSLSPQQDIPLLLP 600
NEEKIPLLMPQH MVLPHYMG+ TELSF+N +SEQDHQKQTT D FSSLSPQ DIPLL+P
Sbjct: 541 NEEKIPLLMPQHDMVLPHYMGKRTELSFKNTDSEQDHQKQTTDDLFSSLSPQDDIPLLMP 600
Query: 601 QEVGGLPDSNIETNSLSMNHNFLNKPMEIQRSVMDSFRTYIVEPLTQYDAQTNGLLDEFG 660
QE GGLPDSN +TN+LSM HN N+PMEI +SV SF+ Y EPLTQ + QTNGLLD+FG
Sbjct: 601 QEAGGLPDSNEQTNNLSMQHNSFNQPMEIGKSVTGSFQAYNAEPLTQIE-QTNGLLDDFG 660
Query: 661 FLDEFDALDHPREATIDAPAYMKTSDNWLETEHESNYVVAIDDVKEIGPLTASNCQIIRS 720
FLDEF A H RE T D P YMK S++WLETE ESN+ VAI++V EIGPLT+SNCQ+IRS
Sbjct: 661 FLDEFGAFGHLREPTFDTPPYMKNSNDWLETECESNH-VAINEVSEIGPLTSSNCQVIRS 720
Query: 721 VSQWSAGTSKPEASIHAAYCFAIQEAKHFIYIE--------------------------- 780
VSQWSAGTSKPEASIHAAYC AIQEAKHFIYIE
Sbjct: 721 VSQWSAGTSKPEASIHAAYCAAIQEAKHFIYIENQFFISGLSGDETIQNRVLDALYQRIW 780
Query: 781 ----------------------GGLDDNGAATVRALMHWQYRSISWEKTSILYHLNLLLG 840
GG+DDNGAATVRALMHWQYR+ISWEKTSILY LNLLLG
Sbjct: 781 LAHKEKQCFRVIVVLPLLPGFQGGVDDNGAATVRALMHWQYRTISWEKTSILYRLNLLLG 840
Query: 841 PKTQDYILFCGLRSYGRLFDGGPIATSQVYVHSKLMIIDDCITFIGSSNINDRSLLGSRD 900
PKTQDYILFCGLRSYGRLFDGGPIATSQ+YVHSKLMIIDDCITFIGSSNINDRSLLGSRD
Sbjct: 841 PKTQDYILFCGLRSYGRLFDGGPIATSQIYVHSKLMIIDDCITFIGSSNINDRSLLGSRD 900
Query: 901 SEIGVIIEDKEFVDSSMNGKPWKAGKFAHSLRCSLWCEHLGLQLGEISQISDPTAEATYK 960
SEIGVIIEDKEFV SSMNGKPWKAGKFAHSLRCSLWCEHLGL LGE+SQI DP EATYK
Sbjct: 901 SEIGVIIEDKEFVVSSMNGKPWKAGKFAHSLRCSLWCEHLGLHLGEVSQILDPVVEATYK 960
Query: 961 DLWVATAKENTAIYKDVFSCIPNDNINSRSSLRQHLADLRGKLDHNTIDLGIAAEKIEIY 1003
DLW+ATAKENT IY++VFSCIPNDNINSRSSLRQ LADL+ KLDHNT+DLGIAAEKIE +
Sbjct: 961 DLWLATAKENTTIYEEVFSCIPNDNINSRSSLRQSLADLKVKLDHNTLDLGIAAEKIESH 1020
BLAST of Sgr012749 vs. TAIR 10
Match:
AT3G16785.1 (phospholipase D P1 )
HSP 1 Score: 1215.7 bits (3144), Expect = 0.0e+00
Identity = 629/1097 (57.34%), Postives = 749/1097 (68.28%), Query Frame = 0
Query: 19 PRLVSASHSFRQC--GEPAWIFEELPVASIVSVSRPDTGDISPLLLSYTIQIQYKQFKWS 78
P +VS+ SF E IFEELP A IVSVSRPD GDISP+LLSYTI+ QYKQFKW
Sbjct: 25 PSMVSSLFSFAPAPTQETNRIFEELPKAVIVSVSRPDAGDISPVLLSYTIECQYKQFKWQ 84
Query: 79 LVKKASQVLYLHFALKRRAFIEELHEKQEQVKEWLQNLGIVDHTAVVHNDDESDVGAFPL 138
LVKKASQV YLHFALK+RAFIEE+HEKQEQVKEWLQNLGI DH VV ++D +V PL
Sbjct: 85 LVKKASQVFYLHFALKKRAFIEEIHEKQEQVKEWLQNLGIGDHPPVVQDEDADEV---PL 144
Query: 139 HDEQTTRNRNVPSVAALPIIKPALGGQRSISDKAKLAMQGYLNHFLGNLDIVNSRE---- 198
H +++ +NR+VPS AALP+I+P LG Q+SIS + K AMQ YLNHFLGNLDIVNSRE
Sbjct: 145 HQDESAKNRDVPSSAALPVIRP-LGRQQSISVRGKHAMQEYLNHFLGNLDIVNSREVCRF 204
Query: 199 ------------------------------------------------------VWAVLK 258
VW VLK
Sbjct: 205 LEVSMLSFSPEYGPKLKEDYIMVKHLPKFSKSDDDSNRCCGCCWFCCCNDNWQKVWGVLK 264
Query: 259 PGFLALVADPLDTKLLDIIVFDVLPTLEGKEGSQANLAYHVKDRNPLRYSFMVRCGNGNM 318
PGFLAL+ DP D KLLDIIVFDVLP G +G +LA +KD NPLR++F V GN ++
Sbjct: 265 PGFLALLEDPFDAKLLDIIVFDVLPVSNGNDGVDISLAVELKDHNPLRHAFKVTSGNRSI 324
Query: 319 KFRTTSAAKVREWVTSINDAGLGPQDGWCHPHRFGSFAPQRGLSDDESRAQWFIDGRAAF 378
+ R ++AKV++WV SINDA L P +GWCHPHRFGS+AP RGL+DD S+AQWF+DG AAF
Sbjct: 325 RIRAKNSAKVKDWVASINDAALRPPEGWCHPHRFGSYAPPRGLTDDGSQAQWFVDGGAAF 384
Query: 379 EAIACSIEAAKSEIFITGWWLCPELYMQRPFHYHSSSRLDALLETKAKEGVKIYILIYKE 438
AIA +IE AKSEIFI GWW+CPELY++RPF H+SSRLD LLE KAK+GV+IYILIYKE
Sbjct: 385 AAIAAAIENAKSEIFICGWWVCPELYLRRPFDPHTSSRLDNLLENKAKQGVQIYILIYKE 444
Query: 439 VPIALKINSMYSKKRLLSIHENIKVLRSPDHMSTGIYYWSHHEKLVIIDHHICFIGGLDL 498
V +ALKINS+YSK+RLL IHEN++VLR PDH S+G+Y WSHHEKLVI+D+ +CFIGGLDL
Sbjct: 445 VALALKINSVYSKRRLLGIHENVRVLRYPDHFSSGVYLWSHHEKLVIVDNQVCFIGGLDL 504
Query: 499 CFGRYDTMEHKVSDFPPYTWPGKDYYNPRESEPNSWEDTMKDELEREKYPRMPWHDVHCA 558
CFGRYDT EHKV D P TWPGKDYYNPRESEPN+WED +KDELER+K+PRMPWHDVHCA
Sbjct: 505 CFGRYDTFEHKVGDNPSVTWPGKDYYNPRESEPNTWEDALKDELERKKHPRMPWHDVHCA 564
Query: 559 LWGSPCRDVARHFVQRWNHAKRNKAPNEEKIPLLMPQHHMVLPHYMGRSTELSFENNNSE 618
LWG PCRDVARHFVQRWN+AKRNKAP E+ IPLLMPQHHMV+PHYMGR E E+ E
Sbjct: 565 LWGPPCRDVARHFVQRWNYAKRNKAPYEDSIPLLMPQHHMVIPHYMGRQEESDIESKKEE 624
Query: 619 QDHQKQTTVDFFSSLSPQQDIPLLLPQE---VGGLPDSNIETNSLSMNHNFLNKPMEIQR 678
+ D FSS S QDIPLLLP E G + E + + N F
Sbjct: 625 DSIRGIRRDDSFSSRSSLQDIPLLLPHEPVDQDGSSGGHKENGTNNRNGPF--------- 684
Query: 679 SVMDSFRTYIVEPLTQYDAQTNGLLDEFGFLDEFDALDHPREATIDAPAYMKTSDNWLET 738
SFR +EP+ +G GF+D+ + LD P + W ET
Sbjct: 685 ----SFRKSKIEPV-------DGDTPMRGFVDDRNGLDLP----VAKRGSNAIDSEWWET 744
Query: 739 EHESNYVVAIDDVKEIGPLTASNCQIIRSVSQWSAGTSKPEASIHAAYCFAIQEAKHFIY 798
+ V + D+ ++GP T+ CQIIRSVSQWSAGTS+ E SIH+AY I +A+HFIY
Sbjct: 745 QDHDYQVGSPDETGQVGPRTSCRCQIIRSVSQWSAGTSQVEESIHSAYRSLIDKAEHFIY 804
Query: 799 IE-------------------------------------------------GGLDDNGAA 858
IE GG+DD+GAA
Sbjct: 805 IENQFFISGLSGDDTVKNRVLEALYKRILRAHNEKKIFRVVVVIPLLPGFQGGIDDSGAA 864
Query: 859 TVRALMHWQYRSISWEKTSILYHLNLLLGPKTQDYILFCGLRSYGRLFDGGPIATSQVYV 918
+VRA+MHWQYR+I SIL +L +G K DYI F GLR+YG+L + GP+ATSQVYV
Sbjct: 865 SVRAIMHWQYRTIYRGHNSILTNLYNTIGVKAHDYISFYGLRAYGKLSEDGPVATSQVYV 924
Query: 919 HSKLMIIDDCITFIGSSNINDRSLLGSRDSEIGVIIEDKEFVDSSMNGKPWKAGKFAHSL 978
HSK+MI+DD IGS+NINDRSLLGSRDSEIGV+IED E VDS M GKPWKAGKF+ SL
Sbjct: 925 HSKIMIVDDRAALIGSANINDRSLLGSRDSEIGVLIEDTELVDSRMAGKPWKAGKFSSSL 984
Query: 979 RCSLWCEHLGLQLGEISQISDPTAEATYKDLWVATAKENTAIYKDVFSCIPNDNINSRSS 1004
R SLW EHLGL+ GEI QI DP +++TYK++W+ATAK NT IY+DVFSC+PND I+SR +
Sbjct: 985 RLSLWSEHLGLRTGEIDQIIDPVSDSTYKEIWMATAKTNTMIYQDVFSCVPNDLIHSRMA 1044
BLAST of Sgr012749 vs. TAIR 10
Match:
AT3G05630.1 (phospholipase D P2 )
HSP 1 Score: 1154.4 bits (2985), Expect = 0.0e+00
Identity = 627/1124 (55.78%), Postives = 734/1124 (65.30%), Query Frame = 0
Query: 1 MSTERLI------PSGTARSEAEPPRLVSASHSFRQCGEPAWIFEELPVASIVSVSRPDT 60
MST++L+ G R +AS S G + IF+ELP A+IVSVSRPDT
Sbjct: 1 MSTDKLLLPNGVKSDGVIRMTRADAAAAAASSSL---GGGSQIFDELPKAAIVSVSRPDT 60
Query: 61 GDISPLLLSYTIQIQYKQFKWSLVKKASQVLYLHFALKRRAFIEELHEKQEQVKEWLQNL 120
D SPLLLSYT+++QYKQFKW+L KKASQVLYLHFALK+R IEELH+KQEQV+EWL +L
Sbjct: 61 TDFSPLLLSYTLELQYKQFKWTLQKKASQVLYLHFALKKRLIIEELHDKQEQVREWLHSL 120
Query: 121 GIVD-HTAVVHNDDESDVGAFPLH-DEQTTRNRNVPSVAALPIIKPALGGQRSISDKAKL 180
GI D +VV +D+E D GA PLH E + +NRNVPS AALPII+P +G ++ D+ +
Sbjct: 121 GIFDMQGSVVQDDEEPDDGALPLHYTEDSIKNRNVPSRAALPIIRPTIGRSETVVDRGRT 180
Query: 181 AMQGYLNHFLGNLDIVNSRE---------------------------------------- 240
AMQGYL+ FLGNLDIVNS+E
Sbjct: 181 AMQGYLSLFLGNLDIVNSKEVCKFLEVSRLSFAREYGSKMKEGYVTVKHLRDVPGSDGVR 240
Query: 241 -----------------VWAVLKPGFLALVADPLDTKLLDIIVFDVLPTLEGKEGS-QAN 300
VWAVLKPGFLAL+ DP KLLDI+VFD L KE S Q
Sbjct: 241 CCLPTHCLGFFGTSWTKVWAVLKPGFLALLEDPFSGKLLDIMVFDTLGLQGTKESSEQPR 300
Query: 301 LAYHVKDRNPLRYSFMVRCGNGNMKFRTTSAAKVREWVTSINDAGLGPQDGWCH-PHRFG 360
LA VK+ NPLR+ F V G+ ++ RTTS+ KV+EWV ++++AG C+ PHRFG
Sbjct: 301 LAEQVKEHNPLRFGFKVTSGDRTVRLRTTSSRKVKEWVKAVDEAG-------CYSPHRFG 360
Query: 361 SFAPQRGLSDDESRAQWFIDGRAAFEAIACSIEAAKSEIFITGWWLCPELYMQRPFHYHS 420
SFAP RGL+ D S+AQWF+DG AFEAIA +I+ A SEIF+TGWWLCPELY++RPF H
Sbjct: 361 SFAPPRGLTSDGSQAQWFVDGHTAFEAIAFAIQNATSEIFMTGWWLCPELYLKRPFEDHP 420
Query: 421 SSRLDALLETKAKEGVKIYILIYKEVPIALKINSMYSKKRLLSIHENIKVLRSPDHMSTG 480
S RLDALLETKAK+GVKIYIL+YKEV IALKINS+YSKKRL +IH+N+KVLR PDH+S+G
Sbjct: 421 SLRLDALLETKAKQGVKIYILLYKEVQIALKINSLYSKKRLQNIHKNVKVLRYPDHLSSG 480
Query: 481 IYYWSHHEKLVIIDHHICFIGGLDLCFGRYDTMEHKVSDFPPYTWPGKDYYNPRESEPNS 540
IY WSHHEK+VI+D+ +CFIGGLDLCFGRYDT EHK+ D PPY WPGKDYYNPRESEPNS
Sbjct: 481 IYLWSHHEKIVIVDYQVCFIGGLDLCFGRYDTAEHKIGDCPPYIWPGKDYYNPRESEPNS 540
Query: 541 WEDTMKDELEREKYPRMPWHDVHCALWGSPCRDVARHFVQRWNHAKRNKAPNEEKIPLLM 600
WE+TMKDEL+R KYPRMPWHDVHCALWG PCRDVARHFVQRWNH+KRNKAPNE+ IPLLM
Sbjct: 541 WEETMKDELDRRKYPRMPWHDVHCALWGPPCRDVARHFVQRWNHSKRNKAPNEQTIPLLM 600
Query: 601 PQHHMVLPHYMGRSTELSFENNNSEQDHQKQTTV---DFFSSLSPQQDIPLLLPQEVGGL 660
P HHMVLPHY+G E+D K + D FSS SP Q+IPLLLPQE
Sbjct: 601 PHHHMVLPHYLGTREIDIIAAAKPEEDPDKPVVLARHDSFSSASPPQEIPLLLPQE---- 660
Query: 661 PDSNIETNSLSMNHNFLNKPMEIQRSVMDSFRTYIVEPLTQYDAQTNGLLDEFGFLDEFD 720
T D G
Sbjct: 661 ---------------------------------------TDADFAGRG------------ 720
Query: 721 ALDHPREATIDAPAYMKTSDNWLETEHESNYVVAIDD-VKEIGPLTASNCQIIRSVSQWS 780
+ +D+ A ET ES+ A++D +IG + CQIIRSVSQWS
Sbjct: 721 ------DLKLDSGARQDPG----ETSEESDLDEAVNDWWWQIGKQSDCRCQIIRSVSQWS 780
Query: 781 AGTSKPEASIHAAYCFAIQEAKHFIYIE-------------------------------- 840
AGTS+PE SIH AYC IQ A+HFIYIE
Sbjct: 781 AGTSQPEDSIHRAYCSLIQNAEHFIYIENQFFISGLEKEDTILNRVLEALYRRILKAHEE 840
Query: 841 -----------------GGLDDNGAATVRALMHWQYRSISWEKTSILYHLNLLLGPKTQD 900
GG+DD GAATVRALMHWQYR+IS E TSIL +LN LLGPKTQD
Sbjct: 841 NKCFRVVIVIPLLPGFQGGIDDFGAATVRALMHWQYRTISREGTSILDNLNALLGPKTQD 900
Query: 901 YILFCGLRSYGRLFDGGPIATSQVYVHSKLMIIDDCITFIGSSNINDRSLLGSRDSEIGV 960
YI F GLRSYGRLF+ GPIATSQ+YVHSKLMI+DD I IGSSNINDRSLLGSRDSEIGV
Sbjct: 901 YISFYGLRSYGRLFEDGPIATSQIYVHSKLMIVDDRIAVIGSSNINDRSLLGSRDSEIGV 960
Query: 961 IIEDKEFVDSSMNGKPWKAGKFAHSLRCSLWCEHLGLQLGEISQISDPTAEATYKDLWVA 1004
+IEDKEFV+SSMNG W AGKF++SLRCSLW EHLGL GEI +I DP +ATYKDLW+A
Sbjct: 961 VIEDKEFVESSMNGMKWMAGKFSYSLRCSLWSEHLGLHAGEIQKIEDPIKDATYKDLWMA 1020
BLAST of Sgr012749 vs. TAIR 10
Match:
AT5G25370.1 (phospholipase D alpha 3 )
HSP 1 Score: 125.2 bits (313), Expect = 3.5e-28
Identity = 152/612 (24.84%), Postives = 252/612 (41.18%), Query Frame = 0
Query: 318 FEAIACSIEAAKSEIFITGWWLCPELYMQRP---FHYHSSSRLDALLETKAKEGVKIYIL 377
+E I +I AK I+I GW + ++ + R +L LL+ KA+E V + +L
Sbjct: 219 WEEIFDAIWEAKHLIYIAGWSVNTDVTLVRDPKRTRPGGDLKLGELLKKKAEENVTVLML 278
Query: 378 IYKEVPIALKINSMYSKKRLLSIHE--------NIKVL-----RSPDHMSTGIY------ 437
++ + + ++ + L+ H+ N KV R+PD+ + +
Sbjct: 279 VWDD----RTSHEVFKRDGLMMTHDQETYDYFKNTKVRCVLCPRNPDNGDSIVQGFEVAT 338
Query: 438 YWSHHEKLVIIDHH----------ICFIGGLDLCFGRYDTMEHKVSDFPPYTWPGKDYYN 497
++HH+K +++D + F+GG+DLC GRYDT+EH + + D++
Sbjct: 339 MFTHHQKTIVVDSEVDGSLTKRRIVSFLGGIDLCDGRYDTVEHPLFG-TLNSVHANDFHQ 398
Query: 498 PRESEPNSWEDTMKDELEREKYPRMPWHDVHCALWGSPCRDVARHFVQRW---NHAKRNK 557
P + ++ PR PWHD+HC L G DV +F QRW +R
Sbjct: 399 PNFDGASI----------KKGGPREPWHDIHCKLDGPAAWDVLYNFEQRWMKQGSGRRYL 458
Query: 558 APNEEKIPLLMPQHHMVLPHYMGRSTELSFENNNSEQDHQKQTTVDFFSSLSPQQDIPLL 617
+ + +P +V P D+++ TV F S+
Sbjct: 459 ISMAQLAEITVPPLPIVQP------------------DNEEGWTVQVFRSID-------- 518
Query: 618 LPQEVGGLPDSNIETNSLSMNHNFLNKPMEIQRSVMDSFRTYIVEPLTQYDAQTNGLLDE 677
V G P+ E S+ + K I+RS+ D++ N +
Sbjct: 519 -DGAVEGFPEDPREAASIGL---ISGKDNVIERSIQDAY--------------VNAIRRA 578
Query: 678 FGFLDEFDALDHPREATIDAPAYMKTSDNWLETEHESNYVVAIDDV-KEIGPLTASNCQI 737
F+ I+ ++ +S W + N + A+ + KEI + +I
Sbjct: 579 KNFI------------YIENQYFLGSSFGWNSRDINLNEINALQLIPKEI------SLKI 638
Query: 738 IRSVSQWSAGTSKPEASIHAAYCFAIQEAKHFIYIEGGLDDNGAATVRALMHWQYRSISW 797
+ SK EA + I ++ EG G+A+V+A++ WQ R++
Sbjct: 639 V----------SKIEAGERFSVYIVIP-----LWPEG---KPGSASVQAILDWQRRTMEM 698
Query: 798 EKTSILYHLNLL-LGPKTQDYILF--CGLRSYGRLFD----GGPIATS-----------Q 857
T I+ L L +DY+ F G R G++ + P A S
Sbjct: 699 MYTDIIIALRKKGLDANPRDYLTFFCLGNREKGKVGEYLPPEKPEANSDYARAQESRRFM 732
Query: 858 VYVHSKLMIIDDCITFIGSSNINDRSLLGSRDSEIGVIIEDKEFVDSSMNGKPWKAGKFA 876
+YVHSK+MI+DD IGS+NIN RS+ G RD+EI + + S+ N +P G+
Sbjct: 759 IYVHSKMMIVDDEYIIIGSANINQRSMDGGRDTEIAMGAYQPSHLLSTNNMRP--VGQI- 732
BLAST of Sgr012749 vs. TAIR 10
Match:
AT1G52570.1 (phospholipase D alpha 2 )
HSP 1 Score: 113.6 bits (283), Expect = 1.1e-24
Identity = 155/656 (23.63%), Postives = 253/656 (38.57%), Query Frame = 0
Query: 290 HRFGSFAPQRGLSDDESRAQWFIDGRAAFEAIACSIEAAKSEIFITGWWLCPELYMQRPF 349
H G+F P+ L+ ++ + +E I +I AK I+ITGW + E+ + R
Sbjct: 189 HIPGNFVPKIPLAGGKN-----YEPHRCWEDIFDAITNAKHLIYITGWSVYTEISLVRDS 248
Query: 350 HYHSSS---RLDALLETKAKEGVKIYILIYKEVPIALKINSMYSKKRLLSIHEN------ 409
+ LL+ KA EGVK+ +L++ + + K L++ H+
Sbjct: 249 RRPKQGGDVTVGELLKKKASEGVKVILLVWDDRTSV----DLLKKDGLMATHDEETENFF 308
Query: 410 -------IKVLRSPDHMSTGIY------YWSHHEKLVIIDHH-----------ICFIGGL 469
I R+PD + + ++HH+K+V++D + F+GGL
Sbjct: 309 RGTDVNCILCPRNPDDGGSIVQNLQISTMFTHHQKIVVVDSEMPSGGSRSRRIVSFVGGL 368
Query: 470 DLCFGRYDTMEHKVSDFPPYTWPGKDYYNPRESEPNSWEDTMKDELEREKYPRMPWHDVH 529
DLC GRYDT H + F D ++ +PN + PR PWHD+H
Sbjct: 369 DLCDGRYDTPFHSL--FRTLDTAHHDDFH----QPN-----FTGAAITKGGPREPWHDIH 428
Query: 530 CALWGSPCRDVARHFVQRWNHAKRNKAPNEEKIPLLMPQHHMVLPHYMGRSTELSFENNN 589
C L G DV +F QRW+
Sbjct: 429 CRLEGPIAWDVLYNFEQRWS---------------------------------------- 488
Query: 590 SEQDHQKQTTVDFFSSLSPQQDIPLLLPQEVGGLPDSNIETNSLSMNHNFLNKPMEIQRS 649
+Q D + DI ++ P V S +H+ N +++ RS
Sbjct: 489 ------RQGGKDILVKMRELGDI-IIPPSPV-----------LFSEDHDVWN--VQLFRS 548
Query: 650 VMDSFRTYIVEPLTQYDAQTNGLLDEFGFLDEFDALDHPREATIDAPAYMKTSDNWLETE 709
+ GF D EA +A + DN ++
Sbjct: 549 IDGGAAA--------------------GFPDS-------PEAAAEA-GLVSGKDNIIDRS 608
Query: 710 HESNYVVAIDDVKEIGPLTASNCQIIRSVSQWSAGTSKPEASIHAAYCF----------- 769
+ Y+ AI K+ + N + S WSA KPE I+A +
Sbjct: 609 IQDAYIHAIRRAKDF--IYIENQYFLGSSFAWSADGIKPE-EINALHLIPKELSLKIVSK 668
Query: 770 --AIQEAKHFIYI---EGGLDDNGAATVRALMHWQYRSISWEKTSILYHL--NLLLGPKT 829
A ++ K ++ + G+ ++G +V+A++ WQ R++ ++ L N L G
Sbjct: 669 IKAGEKFKVYVVVPMWPEGIPESG--SVQAILDWQKRTMEMMYKDVIKALRENGLEGEDP 726
Query: 830 QDYILFCGLRSYGRLFDGGPIATSQ-----------------VYVHSKLMIIDDCITFIG 878
+DY+ F L + DG + + +YVH+K+MI+DD IG
Sbjct: 729 RDYLTFFCLGNREVKKDGEYEPSEKPEPDTDYIRAQEARRFMIYVHTKMMIVDDEYIIIG 726
BLAST of Sgr012749 vs. TAIR 10
Match:
AT4G11830.1 (phospholipase D gamma 2 )
HSP 1 Score: 107.8 bits (268), Expect = 5.8e-23
Identity = 144/591 (24.37%), Postives = 245/591 (41.46%), Query Frame = 0
Query: 318 FEAIACSIEAAKSEIFITGWWLCPELYMQRPFHYHSSSRLDALLETKAKEGVKIYILIYK 377
+E +A +I A+ I+ITGW + + + R + + L LL+ K++EGV++ +L++
Sbjct: 216 WEDMADAIRRARRLIYITGWSVFHPVRLVRRNNDPTEGTLGELLKVKSQEGVRVLVLVWD 275
Query: 378 EVPIALK---------INSMYSKKRLLSIHENIKVLRSPDHMSTGIYY---------WSH 437
+ P ++ +N+ + R H +++VL P + G + ++H
Sbjct: 276 D-PTSMSFPGFSTKGLMNTSDEETRRFFKHSSVQVLLCPRYGGKGHSFIKKSEVETIYTH 335
Query: 438 HEKLVIIDHH--------ICFIGGLDLCFGRYDTMEHKVSDFPPYTWPGKDYYNPRESEP 497
H+K +I+D + F+GGLDLC GR+DT +H + T D++NP
Sbjct: 336 HQKTMIVDAEAAQNRRKIVAFVGGLDLCNGRFDTPKHSLFG-TLKTLHKDDFHNPNFV-- 395
Query: 498 NSWEDTMKDELEREKYPRMPWHDVHCALWGSPCRDVARHFVQRWNHAKR---NKAPNEEK 557
+ ED PR PWHD+H + G DV +F +RW +K K
Sbjct: 396 -TTEDV---------GPREPWHDLHSKIDGPAAYDVLANFEERWMASKPRGIGKGRTSFD 455
Query: 558 IPLLMPQHHMVLPHYMGRSTELSFENNNSEQDH-QKQTTVDFFSSLSPQQDIPLLLPQEV 617
LL +P MG S S +N+ E H Q ++D S +D P+E
Sbjct: 456 DSLLRINR---IPDIMGLSEASSANDNDPESWHVQVFRSIDSTSVKGFPKD-----PEEA 515
Query: 618 GGLPDSNIETNSLSMNHNFLNKPMEIQRSVMDSFRT--YIVEPLTQYDAQTNGLLDEFGF 677
G +L N L M I + + + R+ + + QY ++ D
Sbjct: 516 TG--------RNLLCGKNIL-IDMSIHAAYVKAIRSAQHFIYIENQYFLGSSFNWDSNKD 575
Query: 678 LDEFDALDHPREATIDAPAYMKTSDNWLETEHESNYVVAIDDVKEIGPLTASNCQIIRSV 737
L + + P E + ++ +N+ + Y+V + P A + I+ +
Sbjct: 576 LGANNLI--PMEIALKIANKIRARENF------AAYIVI-----PMWPEGAPTSKPIQRI 635
Query: 738 SQWSAGTSKPEASIHAAYCFAIQEAKHFIYIEGGLDDNGAATVRALMHWQYRSISWEKTS 797
W T + I +A + ++G L+ L + + + +
Sbjct: 636 LYWQHKTMQMMYQ-------TIYKALLEVGLDGQLEPQDFLNFFCLGNREVGTREVPDGT 695
Query: 798 ILYHLNLLLGPKTQDYILFCGLRSYGRLFDGGPIATSQVYVHSKLMIIDDCITFIGSSNI 857
+ + N P + L+S R F +YVHSK M++DD IGS+NI
Sbjct: 696 VNVY-NCPRKPPQPNAAQVQALKS--RRF--------MIYVHSKGMVVDDEFVLIGSANI 741
Query: 858 NDRSLLGSRDSEIGVIIEDKEFVDSSMNGKPWKAGKFAHSLRCSLWCEHLG 877
N RSL G+RD+EI + + +P + F + R SLW EHLG
Sbjct: 756 NQRSLEGTRDTEIAMGGYQPHHSWAKKGSRP-RGQIFGY--RMSLWAEHLG 741
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
Q9LRZ5 | 0.0e+00 | 57.34 | Phospholipase D zeta 1 OS=Arabidopsis thaliana OX=3702 GN=PLDZETA1 PE=1 SV=1 | [more] |
Q9M9W8 | 0.0e+00 | 55.78 | Phospholipase D zeta 2 OS=Arabidopsis thaliana OX=3702 GN=PLPZETA2 PE=1 SV=2 | [more] |
Q0V8L6 | 5.0e-96 | 30.35 | Phospholipase D2 OS=Bos taurus OX=9913 GN=PLD2 PE=2 SV=1 | [more] |
Q9Z280 | 1.9e-95 | 27.95 | Phospholipase D1 OS=Mus musculus OX=10090 GN=Pld1 PE=1 SV=1 | [more] |
O14939 | 2.5e-95 | 30.45 | Phospholipase D2 OS=Homo sapiens OX=9606 GN=PLD2 PE=1 SV=2 | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1DUS1 | 0.0e+00 | 81.80 | Phospholipase OS=Momordica charantia OX=3673 GN=LOC111023278 PE=3 SV=1 | [more] |
A0A6J1DQ37 | 0.0e+00 | 80.80 | Phospholipase OS=Momordica charantia OX=3673 GN=LOC111023278 PE=3 SV=1 | [more] |
A0A6J1G515 | 0.0e+00 | 79.62 | Phospholipase OS=Cucurbita moschata OX=3662 GN=LOC111450778 PE=3 SV=1 | [more] |
A0A6J1KFT8 | 0.0e+00 | 79.53 | Phospholipase OS=Cucurbita maxima OX=3661 GN=LOC111493911 PE=3 SV=1 | [more] |
A0A5D3BB20 | 0.0e+00 | 81.63 | Phospholipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G001390... | [more] |