Sgr012593 (gene) Monk fruit (Qingpiguo) v1

Overview


Name	Sgr012593
Type	gene
Organism	Siraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description	(-)-germacrene D synthase-like
Location	tig00153449: 95856 .. 99629 (+)
RNA-Seq Expression	Sgr012593
Synteny	Sgr012593

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACGATTCGAGGCTTTATAAAAGGAGAAGAAGCTGGGAAAGAACGATCATCAGAGAACAACAAGAATTTTTTAGAGGGAAAAGGAGAGTATTCTAAGACCAAGAAGAGGAATTTTCAAAGCTCGAGATCGATAAAACTGAGTTCGGAGACAAAACCCCAATCCTAGATGTGACAACCCTTTAACTTATCATTATATTAAAATTTGACATCTTACATTTGCTGTTAGTAATTTCTACAATAAAATTAAAAATATTTCTTTTTCTAAAATAAGTAATTGTAAATATAACATTTAAAAAAGATTCTTTAATCAAATTTTGGATTTATAAAATCAATATGTTTTTATTTTACGCAATCATTTTTTGTTTACGTCAGCGAAAGTATAGCTCGATGACTAAGAACATCTCTACTTCTTCTTCTAAGAGGTTATAAGTATAGATCCTCACTTTTATAGTTGTGATATAATATTTTCAACAAGAAAAAAAAAATTGTCTATTCAAAAAGATGATTTGAATGCAACGTTAAATTTTTTGGCCACCTCCGCCGTAGTACTCATCTCCGTCGGCACCTGAAGATTGGCTTCACTTATGAAGTCACAATTTCTCCAGGTCGATGGCATATAACCAAAATTAAAGAAAAATTTTGACTCAATCCAACGGTTGGGAGTCGCTTATCATTTTGAACTGAGATCAATGAAGCGTTGGATTATGTTTATAAGAATTACAATATCATTAGCCACGAAAGAGATGAAGACCTTCATGTCGTTGCTCTTAGGTTTCGCTTGCTTAGACAACAATGATATTCTATTTCATGTGGTAAACTCTCGCTTATTCATCATCAATTTTGTTATCTTTTTGGTTAAATTATAAACTTTGTTGTCTATATTTTTTGAGCATGGTATCTATTTGGTTTCTAAATTTTAAAAAGTTTTAAATTATGTCTTGAAACATAGAGATCTTCAACAAGTTCACCGATGACAAAGGGAATTTCAAGGAATCACTTGCCGAGGATGCACGAGGAACGTTAAGCTTGTACGAGGCATCTCATATATGGGTGCATGGAGAAGACATACTCGAACGAGCCCTAGAGTTCATGACGACGAGTTTAGAAGCAGCCATTGGAAGCCATGCAGTAGGCAGGTGTGGGTTTGCAGCCCAAGTGAGGTATGCATTGAAGTGGCCGCTCATGAAAGCCATGCCTAGGCTTGCAGCAAGACGTTACATATCTTTCTATGAAAAAGATCCATTGCATGATTCTTGCTTTCCTTCGCCAAGCTTGATTATAATTGCTTGCAAAAGCTTTACCACAAAGAACTTCATGAAATCTCAAGGTGAAGATCAATCTCTAGAGTTCATAAATACTAGTACTTCTTTAACCTTTTTTTTTTCTTTCTTTCAAACTACATGCATAGTTTGTGCCTTTAATTGTTAGTATGTTTTTTTTTAACATGACGTTATTGTATGCACTTGAAATCATTGTTTTACAAGCTCAGTGTTTTCGAAGGAGTTGAAAAACACCCAAATTCTTGAGAAGAAGTGAGAGAAATTTTAAAATAGACTGTTCTTTAGAAAAACAACTTTTTGCTTTTTAAAACAAAAATTGAGCGAGGACATGTTTGGATTATTGTTTATTTTTCAGTTGTTTTTCTCCTAGTTTATTTTTACGGATGACTTATCTTTTAGAATAATTAAAGTCGTATTTTCTTTCAAAATCATGAAGTTATAAGTAGTGATTCCAAATGCATGGTAAGCTTTTACTTTTAGAAGTCAACAAAAAAAAAAAAAATCTACTCTTTCCACTTCTTGAGAATGACTACCAAAAAAATTTTTGTTGGATAATCTGAAAACTAATTTCAAAATAATTTAATGTTCTTGTTTTTAGACTATTTATCTAAAAATTGTGATTGAATGACTTTGTAAAAGTTAATTTTATTCGACACGTTCAAATCACTATTCACAAATATGTGATTTTGGAATCAATAATAATTTCAATTTTTTAAAAGTTAAGTCACTTGTGAGAATACATTAAAAAATAAAGAAGAAGAAAACCCTTTTTTTGAAAAACAATAGCTATCCAAACATGTTTCTACAAAACCTTTATTCAAGCTAGAAAACCAAAAATTGTCTTGAAATTACTTACCAAACAATTATCTAAACATATTTTTACTTGAGTTTTAATTTAAGAATGTTGTCTTAAGGACTTTAAAATATTTATGTATACAAATTAGGTGGTGGAAAGACTTGAAGCTGGTGGAAAAGCTGTCTTTTGCAAGAGATAGATGTGTGGAATGTTATATATGGGTATTGGGAATTTACTTTGAGCCTAAGTACTCTCTTGGTAGAAGAATTACAACCAAAATTGTAGTTATTACTTCGGTTTTGGATGATATATACGATCTATATGGGACATTTGAAGAACTTCAACTCTTCACTCGTGCAATTGAAAGGTTTGGTTGACTCATTTCAAGTTCTGTTTGATAACGATTTTGTTTTTAAAAAACCAAACTTGATTCTTTTTAAATATAGGCCAAGTTTCTTTGCTTTGTTTTACATTTAGCTTTTTTTAAAAAAATTTAAAATTATTGAAAAATTTTGAAAATTGCTTTTTAGTTTTTAAATATAGCTAGGAAGGACTAAATACAAGTTGAAGAACCATAATTAAAATTTTTAAAGTTTGAGGACTAAATAGATATGATATTTAAAGTAGAGAGATTAAATTTAAAATTTAACTATAAAAAAATTATTAGTAATTTAATAGATACCACATTTTTTCAAAATGAAATCATTATCAAGTGGAACATAATTGCCTAATGCAAAGTAAGTGTCATGCAAATGACAAGTTATGTGTTATCTCTCTATTATTTTTTTTTTTCGACTTTTTAGGTGGGATGGTAACTGCATTGAGGAACTTCCAAAATAAATGAAAGTGTATTATGAAGCTCTAATGGACACTTATAGAGGAATTGAATTGTGCAAGATATTAGCAAAGATGACATCCCTTATGCCATTCATTATGCTAAAGAAGCTGTATATTCTCTCTTACCTTATATATTCTATAGTCTCATAATAAAAATAGTAGTTCTCTCTCTAACACATTTTAAGGTAGAATTTGACATATTCCACTAAGCATAAAGAGTAAAAAAAAATGGGTTTTTTTTAATCTATTAATAAGTGTCTTCAGAGCAAAAATTTAGGGAATTAAATGTGTTTGAGTCACTCTTTTTTAAAAGTTATTTTTCCCTTGGTTTGTTTTAGTTTATTATTATATGTATTGCTTACTGTCCAAAACCATTGGCTTATAAATAGTAATTTGAAGTACATAATAATTTGAACCTTTACAAACTACTCGTTCAAAATTCTTAAAACTAGTTTTGAAAACAAAACCAAAAAATAGCAACCTCCCAAACATATGAGCTTAGTTTATGGAACTCTTTGATCCACATTTCTCTCCCTGCTCTTAAATTTTTTTGTTTGATATATAGTTTCAATAAGAGAGAGGTCATGTCGCTTCAGCCGTTCAATGTTATATGACACAATATGGTGTATCAGAAAAAGAGGCTATACAAGAATTACAGAAGCAAGTTACAAATGCTTGGAAGGAAATGATCGAAGATTACGTGCAATCCATTGATGTTCCAAACACTATTCTTATGCTTATTGTAAATCTTTCTCGACCCTCAGATACCTTTTATAAAGATGAAGATGGATACACATATACAGATGGGGAAACCAAACTTACTGTCATATCTTTACTTGTTGATCCATTGCCAATTTAA

mRNA sequence

ATGACGATTCGAGGCTTTATAAAAGGAGAAGAAGCTGGGAAAGAACGATCATCAGAGAACAACAAGAATTTTTTAGAGGGAAAAGGAGAGTATTCTAAGACCAAGAAGAGGAATTTTCAAAGCTCGAGATCGATAAAACTGAAGATCTTCAACAAGTTCACCGATGACAAAGGGAATTTCAAGGAATCACTTGCCGAGGATGCACGAGGAACGTTAAGCTTGTACGAGGCATCTCATATATGGGTGCATGGAGAAGACATACTCGAACGAGCCCTAGAGTTCATGACGACGAGTTTAGAAGCAGCCATTGGAAGCCATGCAGTAGGCAGGTGTGGGTTTGCAGCCCAAGTGAGGTATGCATTGAAGTGGCCGCTCATGAAAGCCATGCCTAGGCTTGCAGCAAGACGTTACATATCTTTCTATGAAAAAGATCCATTGCATGATTCTTGCTTTCCTTCGCCAAGCTTGATTATAATTGCTTGCAAAAGCTTTACCACAAAGAACTTCATGAAATCTCAAGAAAAAGAGGCTATACAAGAATTACAGAAGCAAGTTACAAATGCTTGGAAGGAAATGATCGAAGATTACGTGCAATCCATTGATGTTCCAAACACTATTCTTATGCTTATTGTAAATCTTTCTCGACCCTCAGATACCTTTTATAAAGATGAAGATGGATACACATATACAGATGGGGAAACCAAACTTACTGTCATATCTTTACTTGTTGATCCATTGCCAATTTAA

Coding sequence (CDS)

ATGACGATTCGAGGCTTTATAAAAGGAGAAGAAGCTGGGAAAGAACGATCATCAGAGAACAACAAGAATTTTTTAGAGGGAAAAGGAGAGTATTCTAAGACCAAGAAGAGGAATTTTCAAAGCTCGAGATCGATAAAACTGAAGATCTTCAACAAGTTCACCGATGACAAAGGGAATTTCAAGGAATCACTTGCCGAGGATGCACGAGGAACGTTAAGCTTGTACGAGGCATCTCATATATGGGTGCATGGAGAAGACATACTCGAACGAGCCCTAGAGTTCATGACGACGAGTTTAGAAGCAGCCATTGGAAGCCATGCAGTAGGCAGGTGTGGGTTTGCAGCCCAAGTGAGGTATGCATTGAAGTGGCCGCTCATGAAAGCCATGCCTAGGCTTGCAGCAAGACGTTACATATCTTTCTATGAAAAAGATCCATTGCATGATTCTTGCTTTCCTTCGCCAAGCTTGATTATAATTGCTTGCAAAAGCTTTACCACAAAGAACTTCATGAAATCTCAAGAAAAAGAGGCTATACAAGAATTACAGAAGCAAGTTACAAATGCTTGGAAGGAAATGATCGAAGATTACGTGCAATCCATTGATGTTCCAAACACTATTCTTATGCTTATTGTAAATCTTTCTCGACCCTCAGATACCTTTTATAAAGATGAAGATGGATACACATATACAGATGGGGAAACCAAACTTACTGTCATATCTTTACTTGTTGATCCATTGCCAATTTAA

Protein sequence

MTIRGFIKGEEAGKERSSENNKNFLEGKGEYSKTKKRNFQSSRSIKLKIFNKFTDDKGNFKESLAEDARGTLSLYEASHIWVHGEDILERALEFMTTSLEAAIGSHAVGRCGFAAQVRYALKWPLMKAMPRLAARRYISFYEKDPLHDSCFPSPSLIIIACKSFTTKNFMKSQEKEAIQELQKQVTNAWKEMIEDYVQSIDVPNTILMLIVNLSRPSDTFYKDEDGYTYTDGETKLTVISLLVDPLPI

Homology

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BLAST of Sgr012593 vs. NCBI nr
Match: XP_022154020.1 ((+)-gamma-cadinene synthase-like [Momordica charantia])

HSP 1 Score: 201.1 bits (510), Expect = 1.2e-47
Identity = 141/371 (38.01%), Postives = 166/371 (44.74%), Query Frame = 0


Query: 40  QSSRSIKLKIFNKFTDDKGNFKESLAEDARGTLSLYEASHIWVHGEDILERALEFMTTSL 99
           Q   SI   IF+KFTDD+GNFKESLA+D RG LSLYEASH+ +HGED+LERAL F TT+L
Sbjct: 130 QQRYSISCDIFDKFTDDEGNFKESLAKDVRGMLSLYEASHVRIHGEDVLERALSFSTTNL 189

Query: 100 EAAI--GSHAVGRCGFAAQVRYALKWPLMKAMPRLAARRYISFYEKDPLHDSCFPSPSLI 159
           +AAI  GSH  G CG   QVR+ALKWP+ KAMPRLAAR YISFYE+DPLH      P+L+
Sbjct: 190 KAAISNGSHE-GSCGIGGQVRHALKWPVFKAMPRLAARSYISFYEQDPLH-----HPTLL 249

Query: 160 IIACKSFT---------------------------------------------------- 219
            +A   +                                                     
Sbjct: 250 YLAKLDYNILQKLYQKELHEISRWDDVNCIEGLPKYMKVYYEALMDTYRGIEHDISKDDI 309

Query: 220 ------TKNFMKSQ---------------------------------------------- 249
                  K  MK Q                                              
Sbjct: 310 SYALHYAKEAMKRQARNYFFEAKWYHDGYTPTIEEYLRVGRVTACYNLFSPISFLGMGDV 369

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BLAST of Sgr012593 vs. NCBI nr
Match: XP_022154019.1 ((-)-germacrene D synthase-like [Momordica charantia])

HSP 1 Score: 187.6 bits (475), Expect = 1.3e-43
Identity = 148/437 (33.87%), Postives = 173/437 (39.59%), Query Frame = 0


Query: 40  QSSRSIKLKIFNKFTDDKGNFKESLAEDARGTLSLYEASHIWVHGEDILERALEFMTTSL 99
           Q   SI  +IF+KFTDD+GNFKESLA+D RG LSLYEASH+ +HGED+LERAL F TT+L
Sbjct: 130 QQRYSISCEIFDKFTDDEGNFKESLAKDVRGMLSLYEASHVRMHGEDVLERALAFSTTNL 189

Query: 100 EAAI--GSHAVGRCGFAAQVRYALKWPLMKAMPRLAARRYISFYEKDPLH---------- 159
           +AAI  GSH  G  GF AQVR+ALKWP+ KAMPRLAAR YISFYE+DPLH          
Sbjct: 190 KAAISNGSHE-GSWGFGAQVRHALKWPVFKAMPRLAARNYISFYEQDPLHHPTLLSFAKL 249

Query: 160 ---------------------------------DSC------------------------ 219
                                            D C                        
Sbjct: 250 DYNTLQKLYQRELHEITRWWKDLKLVENLCFARDRCVECYTWALGTYFEPKYSLGRIMLS 309

Query: 220 ------------------FPSPSLIIIACKSFT--------------------------- 249
                             F    L I A   +                            
Sbjct: 310 KIVVITSVLDDIFDLHGTFEELQLFIHAIDRWDVNCIERLPKYMKVYYEAVMDTYRGIEQ 369

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BLAST of Sgr012593 vs. NCBI nr
Match: XP_022154012.1 ((-)-germacrene D synthase-like [Momordica charantia])

HSP 1 Score: 156.0 bits (393), Expect = 4.2e-34
Identity = 87/156 (55.77%), Postives = 110/156 (70.51%), Query Frame = 0


Query: 40  QSSRSIKLKIFNKFTDDKGNFKESLAEDARGTLSLYEASHIWVHGEDILERALEFMTTSL 99
           Q   SI  +IF+KFT+D+GNFKESLA + RG LSLYEASH+ +HGED+LERAL F T +L
Sbjct: 130 QQRYSISCEIFDKFTNDEGNFKESLANEVRGMLSLYEASHMRMHGEDVLERALTFSTINL 189

Query: 100 EAAI--GSHAVGRCGFAAQVRYALKWPLMKAMPRLAARRYISFYEKDPLHDSCFPSPSLI 159
           EAAI  GSH  G CGF AQVR+ALKWP+ KAMPRLAAR YISFYE+DPLH      P+L+
Sbjct: 190 EAAINNGSHE-GSCGFGAQVRHALKWPVFKAMPRLAARSYISFYEEDPLH-----HPALL 249

Query: 160 IIACKSFTTKNFMKSQEKEAIQELQKQ-VTNAWKEM 193
            +A   F   N ++   ++ + E+ +      WKE+
Sbjct: 250 SMAKLDF---NILQKLYQKELHEISRMCCLKWWKEL 276

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BLAST of Sgr012593 vs. NCBI nr
Match: XP_038905736.1 ((-)-germacrene D synthase-like [Benincasa hispida])

HSP 1 Score: 149.8 bits (377), Expect = 3.0e-32
Identity = 129/434 (29.72%), Postives = 155/434 (35.71%), Query Frame = 0


Query: 40  QSSRSIKLKIFNKFTDDKGNFKESLAEDARGTLSLYEASHIWVHGEDILERALEFMTTSL 99
           Q    I   IF+KF DD+GNFKESL +D RG LSLY+ASH+ +HGED+LERAL F T +L
Sbjct: 136 QQRYPISCDIFDKFIDDEGNFKESLVKDVRGMLSLYDASHMQMHGEDVLERALAFTTANL 195

Query: 100 EAAIGSHAVGRCGFAAQVRYALKWPLMKAMPRLAARRYISFYEKDPLHDS---------- 159
            A I +          + RYALKWPL KAMPRLAA+ YISFYEKDPLH+           
Sbjct: 196 RAIINN---SNASHGEEGRYALKWPLFKAMPRLAAKNYISFYEKDPLHNPTLLSFAKLDY 255

Query: 160 --------------------------------------------CFP---------SPSL 219
                                                       CF          +  +
Sbjct: 256 NCLQKLYQKELHEISRWWKDLKLVEKLCFARDRCVECYIWALGLCFEPKYSLARKITSKI 315

Query: 220 IIIA---------------CKSFT------------------------------------ 249
            +IA                + FT                                    
Sbjct: 316 AMIASILDDIYDAYGTFQELQQFTHAIQRWDVNCIEGLPKYMKVCYKALMDIYREIEQDI 375

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BLAST of Sgr012593 vs. NCBI nr
Match: XP_008448865.1 (PREDICTED: (-)-germacrene D synthase-like [Cucumis melo])

HSP 1 Score: 144.8 bits (364), Expect = 9.8e-31
Identity = 129/441 (29.25%), Postives = 161/441 (36.51%), Query Frame = 0


Query: 40  QSSRSIKLKIFNKFTDDKGNFKESLAEDARGTLSLYEASHIWVHGEDILERALEFMTTSL 99
           Q   SI   IFNKFTDD+GNFKE+L +D +G LSLY+ASH+ +HGE+ILERA  F TT+L
Sbjct: 133 QQRYSISCDIFNKFTDDEGNFKETLTKDVKGMLSLYQASHMRMHGEEILERAFAFTTTNL 192

Query: 100 EAAI----GSHAVGRCGFAAQVRYALKWPLMKAMPRLAARRYISFYEKDPLH-------- 159
            AAI    GSH         ++ YALKWP  KAMPRLA+R YIS YE+DPLH        
Sbjct: 193 RAAINNSNGSHE------DREISYALKWPFFKAMPRLASRNYISSYEEDPLHNPTLLCFA 252

Query: 160 ------------------------------------------------------------ 219
                                                                       
Sbjct: 253 KLDYNCLQKLYQKELHEISRWWKELKLMEKLSFARDRCVECYIWALGIYFEPKYSLGRTV 312

Query: 220 -----------------------------------DSC---------------------- 249
                                              D+C                      
Sbjct: 313 VTKVVVLTSIMDDIYDLHATFEELQLLTHAIERWDDTCIKGLPEYMKIYYEALLDTYKGI 372

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BLAST of Sgr012593 vs. ExPASy Swiss-Prot
Match: B2KSJ5 ((+)-gamma-cadinene synthase OS=Cucumis melo OX=3656 PE=1 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 2.3e-22
Identity = 55/109 (50.46%), Postives = 70/109 (64.22%), Query Frame = 0


Query: 40  QSSRSIKLKIFNKFTDDKGNFKESLAEDARGTLSLYEASHIWVHGEDILERALEFMTTSL 99
           Q    I   IF KF DD G FKESL ED RG LSLYEASH+  HGE +LE ALEF TT L
Sbjct: 136 QQGYRISCDIFLKFMDDNGKFKESLVEDERGLLSLYEASHMMGHGEALLEEALEFTTTHL 195

Query: 100 EAAIGSHAVGRCGFAAQVRYALKWPLMKAMPRLAARRYISFYEKDPLHD 149
           +  I  ++     FA++V  ALK P+ K++PR+ AR Y+  Y++ P H+
Sbjct: 196 QTYIHRYSNINPSFASEVSNALKLPIRKSVPRIKAREYLEIYQQHPSHN 244

HSP 2 Score: 69.7 bits (169), Expect = 5.2e-11
Identity = 35/74 (47.30%), Postives = 47/74 (63.51%), Query Frame = 0


Query: 174 EKEAIQELQKQVTNAWKEMIEDYVQSIDVPNTILMLIVNLSRPSDTFYKDEDGYTYTDGE 233
           E+EA  EL K+V +AWK+  E + +  +VP  +LM ++N SR  +  Y DEDGYT     
Sbjct: 497 EEEACIELHKEVVDAWKDTNEAFYRPFNVPVPVLMRVLNFSRVINLLYLDEDGYTNAKSG 556

Query: 234 TKLTVISLLVDPLP 248
           TK  + SLLVDPLP
Sbjct: 557 TKFLIKSLLVDPLP 570

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BLAST of Sgr012593 vs. ExPASy Swiss-Prot
Match: Q6Q3H2 (Valencene synthase OS=Vitis vinifera OX=29760 GN=ValCS PE=1 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 3.0e-22
Identity = 66/153 (43.14%), Postives = 88/153 (57.52%), Query Frame = 0


Query: 40  QSSRSIKLKIFNKFTDDKGNFKESLAEDARGTLSLYEASHIWVHGEDILERALEFMTTSL 99
           Q   +I   IFNKFTD++G FKE+L  D RG L LYEA+H+ VHGEDIL +AL F TT L
Sbjct: 126 QQGYTISCDIFNKFTDERGRFKEALISDVRGMLGLYEAAHLRVHGEDILAKALAFTTTHL 185

Query: 100 EAAIGSHAVGRCGFAAQVRYALKWPLMKAMPRLAARRYISFYEKDPLHDSCFPSPSLIII 159
           +A + S        A QV +AL  P+ K + RL AR YIS Y+ +  HD      +L+ +
Sbjct: 186 KAMVESLGY---HLAEQVAHALNRPIRKGLERLEARWYISVYQDEAFHDK-----TLLEL 245

Query: 160 ACKSFTTKNFMKSQEKEAIQELQKQVTNAWKEM 193
           A   F   N ++S  KE +  L +     WKE+
Sbjct: 246 AKLDF---NLVQSLHKEELSNLARW----WKEL 263

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BLAST of Sgr012593 vs. ExPASy Swiss-Prot
Match: Q6Q3H3 ((-)-germacrene D synthase OS=Vitis vinifera OX=29760 GN=VIT_19s0014g04930 PE=1 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 2.5e-21
Identity = 55/109 (50.46%), Postives = 70/109 (64.22%), Query Frame = 0


Query: 40  QSSRSIKLKIFNKFTDDKGNFKESLAEDARGTLSLYEASHIWVHGEDILERALEFMTTSL 99
           Q    +   +FN F D++GNFKESL+ D RG LSLYEA+H  VHGEDIL+ AL F TT L
Sbjct: 126 QQGYHVSCDLFNNFKDNEGNFKESLSSDVRGMLSLYEATHFRVHGEDILDEALAFTTTHL 185

Query: 100 EAAIGSHAVGRCGFAAQVRYALKWPLMKAMPRLAARRYISFYEKDPLHD 149
           ++A   H+      A QV +ALK P+ K +PRL AR Y S Y+ D  H+
Sbjct: 186 QSAT-KHSSN--PLAEQVVHALKQPIRKGLPRLEARHYFSVYQADDSHN 231

HSP 2 Score: 62.4 bits (150), Expect = 8.4e-09
Identity = 32/77 (41.56%), Postives = 47/77 (61.04%), Query Frame = 0


Query: 172 SQEKEAIQELQKQVTNAWKEMIEDYVQSIDVPNTILMLIVNLSRPSDTFYKDEDGYTYTD 231
           + E+E   E  + V  AWK++ E+ +    VP  ILM ++NL+R  D  YK+EDGYT+  
Sbjct: 481 ASEQETPNEFPQPVREAWKDINEECLIPTAVPMPILMRVLNLARVIDVIYKNEDGYTHFG 540

Query: 232 GETKLTVISLLVDPLPI 249
              K  V S+L+DP+PI
Sbjct: 541 AVLKDFVTSMLIDPVPI 557

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BLAST of Sgr012593 vs. ExPASy Swiss-Prot
Match: Q94JS8 ((E)-beta-farnesene synthase OS=Citrus junos OX=135197 PE=1 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 1.2e-20
Identity = 51/111 (45.95%), Postives = 71/111 (63.96%), Query Frame = 0


Query: 40  QSSRSIKLKIFNKFTDDKGNFKESLAEDARGTLSLYEASHIWVHGEDILERALEFMTTSL 99
           Q    I   +F+KF DD+G FKESL  D RG LSLYEA+++ + GEDIL+ A+ F TT L
Sbjct: 122 QQGVKISCDVFDKFKDDEGKFKESLINDIRGMLSLYEAAYLAIRGEDILDEAIVFTTTHL 181

Query: 100 EA--AIGSHAVGRCGFAAQVRYALKWPLMKAMPRLAARRYISFYEKDPLHD 149
           ++  +I  H+      A Q+R++L+ PL KA  RL AR ++  Y +D LHD
Sbjct: 182 KSVISISDHSHANSNLAEQIRHSLQIPLRKAAARLEARYFLDIYSRDDLHD 232

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BLAST of Sgr012593 vs. ExPASy Swiss-Prot
Match: K4L9M2 (Sesquiterpene synthase 1 OS=Thapsia garganica OX=79022 GN=STS1 PE=1 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 4.7e-20
Identity = 54/108 (50.00%), Postives = 67/108 (62.04%), Query Frame = 0


Query: 40  QSSRSIKLKIFNKFTDDKGNFKESLAEDARGTLSLYEASHIWVHGEDILERALEFMTTSL 99
           Q   +I   +F+KF D  G FKE L ED RG LSL+EA+H+ VHGE+ILE ALEF TT L
Sbjct: 128 QEGHNISSDVFSKFQDSDGKFKEELVEDVRGMLSLFEAAHLRVHGENILEDALEFTTTHL 187

Query: 100 EAAIGSHAVGRCGFAAQVRYALKWPLMKAMPRLAARRYISFYEKDPLH 148
            + + S+       A  VR ALK+PL K+  R+ AR YIS Y K   H
Sbjct: 188 NSYLNSNP--SSSLADLVRRALKYPLRKSFNRMVARHYISVYHKFDWH 233

HSP 2 Score: 52.0 bits (123), Expect = 1.1e-05
Identity = 27/83 (32.53%), Postives = 46/83 (55.42%), Query Frame = 0


Query: 169 FMKSQE---KEAIQELQKQVTNAWKEMIEDYVQSIDVPNTILMLIVNLSRPSDTFYKDED 228
           +MKS +   + A  ELQK +  AWK+M E  +     P  +L  ++NL+R  +  Y   D
Sbjct: 479 YMKSHDVTKETAYAELQKPIIKAWKDMNEGCLHPEAPPKPLLERVLNLARVLNFLYDGHD 538

Query: 229 GYTYTDGETKLTVISLLVDPLPI 249
           GYT++   TK  + +  ++P+P+
Sbjct: 539 GYTHSSTRTKDIITTAFINPIPL 561

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BLAST of Sgr012593 vs. ExPASy TrEMBL
Match: A0A6J1DIG3 ((+)-gamma-cadinene synthase-like OS=Momordica charantia OX=3673 GN=LOC111021378 PE=4 SV=1)

HSP 1 Score: 201.1 bits (510), Expect = 5.6e-48
Identity = 141/371 (38.01%), Postives = 166/371 (44.74%), Query Frame = 0


Query: 40  QSSRSIKLKIFNKFTDDKGNFKESLAEDARGTLSLYEASHIWVHGEDILERALEFMTTSL 99
           Q   SI   IF+KFTDD+GNFKESLA+D RG LSLYEASH+ +HGED+LERAL F TT+L
Sbjct: 130 QQRYSISCDIFDKFTDDEGNFKESLAKDVRGMLSLYEASHVRIHGEDVLERALSFSTTNL 189

Query: 100 EAAI--GSHAVGRCGFAAQVRYALKWPLMKAMPRLAARRYISFYEKDPLHDSCFPSPSLI 159
           +AAI  GSH  G CG   QVR+ALKWP+ KAMPRLAAR YISFYE+DPLH      P+L+
Sbjct: 190 KAAISNGSHE-GSCGIGGQVRHALKWPVFKAMPRLAARSYISFYEQDPLH-----HPTLL 249

Query: 160 IIACKSFT---------------------------------------------------- 219
            +A   +                                                     
Sbjct: 250 YLAKLDYNILQKLYQKELHEISRWDDVNCIEGLPKYMKVYYEALMDTYRGIEHDISKDDI 309

Query: 220 ------TKNFMKSQ---------------------------------------------- 249
                  K  MK Q                                              
Sbjct: 310 SYALHYAKEAMKRQARNYFFEAKWYHDGYTPTIEEYLRVGRVTACYNLFSPISFLGMGDV 369

[Close]

BLAST of Sgr012593 vs. ExPASy TrEMBL
Match: A0A6J1DKT6 ((-)-germacrene D synthase-like OS=Momordica charantia OX=3673 GN=LOC111021377 PE=4 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 6.4e-44
Identity = 148/437 (33.87%), Postives = 173/437 (39.59%), Query Frame = 0


Query: 40  QSSRSIKLKIFNKFTDDKGNFKESLAEDARGTLSLYEASHIWVHGEDILERALEFMTTSL 99
           Q   SI  +IF+KFTDD+GNFKESLA+D RG LSLYEASH+ +HGED+LERAL F TT+L
Sbjct: 130 QQRYSISCEIFDKFTDDEGNFKESLAKDVRGMLSLYEASHVRMHGEDVLERALAFSTTNL 189

Query: 100 EAAI--GSHAVGRCGFAAQVRYALKWPLMKAMPRLAARRYISFYEKDPLH---------- 159
           +AAI  GSH  G  GF AQVR+ALKWP+ KAMPRLAAR YISFYE+DPLH          
Sbjct: 190 KAAISNGSHE-GSWGFGAQVRHALKWPVFKAMPRLAARNYISFYEQDPLHHPTLLSFAKL 249

Query: 160 ---------------------------------DSC------------------------ 219
                                            D C                        
Sbjct: 250 DYNTLQKLYQRELHEITRWWKDLKLVENLCFARDRCVECYTWALGTYFEPKYSLGRIMLS 309

Query: 220 ------------------FPSPSLIIIACKSFT--------------------------- 249
                             F    L I A   +                            
Sbjct: 310 KIVVITSVLDDIFDLHGTFEELQLFIHAIDRWDVNCIERLPKYMKVYYEAVMDTYRGIEQ 369

[Close]

BLAST of Sgr012593 vs. ExPASy TrEMBL
Match: A0A6J1DKI4 ((-)-germacrene D synthase-like OS=Momordica charantia OX=3673 GN=LOC111021373 PE=4 SV=1)

HSP 1 Score: 156.0 bits (393), Expect = 2.1e-34
Identity = 87/156 (55.77%), Postives = 110/156 (70.51%), Query Frame = 0


Query: 40  QSSRSIKLKIFNKFTDDKGNFKESLAEDARGTLSLYEASHIWVHGEDILERALEFMTTSL 99
           Q   SI  +IF+KFT+D+GNFKESLA + RG LSLYEASH+ +HGED+LERAL F T +L
Sbjct: 130 QQRYSISCEIFDKFTNDEGNFKESLANEVRGMLSLYEASHMRMHGEDVLERALTFSTINL 189

Query: 100 EAAI--GSHAVGRCGFAAQVRYALKWPLMKAMPRLAARRYISFYEKDPLHDSCFPSPSLI 159
           EAAI  GSH  G CGF AQVR+ALKWP+ KAMPRLAAR YISFYE+DPLH      P+L+
Sbjct: 190 EAAINNGSHE-GSCGFGAQVRHALKWPVFKAMPRLAARSYISFYEEDPLH-----HPALL 249

Query: 160 IIACKSFTTKNFMKSQEKEAIQELQKQ-VTNAWKEM 193
            +A   F   N ++   ++ + E+ +      WKE+
Sbjct: 250 SMAKLDF---NILQKLYQKELHEISRMCCLKWWKEL 276

[Close]

BLAST of Sgr012593 vs. ExPASy TrEMBL
Match: A0A1S3BLL1 ((-)-germacrene D synthase-like OS=Cucumis melo OX=3656 GN=LOC103490898 PE=4 SV=1)

HSP 1 Score: 144.8 bits (364), Expect = 4.7e-31
Identity = 129/441 (29.25%), Postives = 161/441 (36.51%), Query Frame = 0


Query: 40  QSSRSIKLKIFNKFTDDKGNFKESLAEDARGTLSLYEASHIWVHGEDILERALEFMTTSL 99
           Q   SI   IFNKFTDD+GNFKE+L +D +G LSLY+ASH+ +HGE+ILERA  F TT+L
Sbjct: 133 QQRYSISCDIFNKFTDDEGNFKETLTKDVKGMLSLYQASHMRMHGEEILERAFAFTTTNL 192

Query: 100 EAAI----GSHAVGRCGFAAQVRYALKWPLMKAMPRLAARRYISFYEKDPLH-------- 159
            AAI    GSH         ++ YALKWP  KAMPRLA+R YIS YE+DPLH        
Sbjct: 193 RAAINNSNGSHE------DREISYALKWPFFKAMPRLASRNYISSYEEDPLHNPTLLCFA 252

Query: 160 ------------------------------------------------------------ 219
                                                                       
Sbjct: 253 KLDYNCLQKLYQKELHEISRWWKELKLMEKLSFARDRCVECYIWALGIYFEPKYSLGRTV 312

Query: 220 -----------------------------------DSC---------------------- 249
                                              D+C                      
Sbjct: 313 VTKVVVLTSIMDDIYDLHATFEELQLLTHAIERWDDTCIKGLPEYMKIYYEALLDTYKGI 372

[Close]

BLAST of Sgr012593 vs. ExPASy TrEMBL
Match: A0A6J1CQG9 ((-)-germacrene D synthase-like OS=Momordica charantia OX=3673 GN=LOC111013811 PE=4 SV=1)

HSP 1 Score: 141.7 bits (356), Expect = 4.0e-30
Identity = 127/434 (29.26%), Postives = 155/434 (35.71%), Query Frame = 0


Query: 40  QSSRSIKLKIFNKFTDDKGNFKESLAEDARGTLSLYEASHIWVHGEDILERALEFMTTSL 99
           Q   SI     NKFTD+  NF ES A DA+G LSLYEAS + +HGEDILER   F TTSL
Sbjct: 311 QRGFSISCDTLNKFTDENRNFNESFANDAQGLLSLYEASQMRMHGEDILERGFGFSTTSL 370

Query: 100 EAAIGSHAVGRCGFAAQVRYALKWPLMKAMPRLAARRYISFYEKDPLHDS---------- 159
           +AAI SH     GFA QVR+ALKWPLMKA+PR   R YISFYE DPLH            
Sbjct: 371 KAAIASHV--EDGFATQVRHALKWPLMKAIPRFGIRHYISFYETDPLHHPVLLSFAKLDF 430

Query: 160 --------------------------------------------CF-PSPSLI------I 219
                                                       CF P  SL       I
Sbjct: 431 GVLQRLYNKELREISRWWKDLKLVEKLSFARDRCVECYIWAMAICFEPKYSLARIITSKI 490

Query: 220 IACKS-----------------FT------------------------------------ 249
           I  +S                 FT                                    
Sbjct: 491 IMMQSILDDIYDAYGTFEELQLFTRAIERWNVKCIDELPDYMKVYYEALMDIYRGIEQDI 550

[Close]

BLAST of Sgr012593 vs. TAIR 10
Match: AT5G23960.2 (terpene synthase 21 )

HSP 1 Score: 83.2 bits (204), Expect = 3.3e-16
Identity = 64/217 (29.49%), Postives = 103/217 (47.47%), Query Frame = 0


Query: 49  IFNKFTDDKGNFKESLAEDARGTLSLYEASHIWVHGEDILERALEFMTTSLEAAIGSHAV 108
           +F KF D+ G FK  L  DA G LSLYEA+    HGEDI++ AL F  + LE  I S + 
Sbjct: 124 VFEKFKDENGKFKGHLVTDAYGMLSLYEAAQWGTHGEDIIDEALAFSRSHLE-EISSRSS 183

Query: 109 GRCGFAAQVRYALKWPLMKAMPRLAARRYISFYEKDPLHDSCFPSPSLIIIACKSFTTKN 168
                A +++ ALK P  K + R+  R+YIS+YE++   +SC   P+L+  A   F    
Sbjct: 184 PH--LAIRIKNALKHPYHKGISRIETRQYISYYEEE---ESC--DPTLLEFAKIDFNLLQ 243

Query: 169 FMKSQEKEAI------QELQKQVTNAWKEMIEDYVQSID------------VPNTILMLI 228
            +  +E   +       E + +VT     + E Y+ S+             +    L+L 
Sbjct: 244 ILHREELACVTRWHHEMEFKSKVTYTRHRITEAYLWSLGTYFEPQYSQARVITTMALILF 303

Query: 229 VNLSRPSDTFYKDEDGYTYTDGETKLTVISLLVDPLP 248
             L    D +   E+   +TD   + ++ +++  P+P
Sbjct: 304 TALDDMYDAYGTMEELELFTDAMDEYSICNII--PIP 330

[Close]

BLAST of Sgr012593 vs. TAIR 10
Match: AT3G14490.1 (Terpenoid cyclases/Protein prenyltransferases superfamily protein )

HSP 1 Score: 81.3 bits (199), Expect = 1.2e-15
Identity = 56/144 (38.89%), Postives = 72/144 (50.00%), Query Frame = 0


Query: 50  FNKFTDDKGNFKESLAEDARGTLSLYEASHIWVHGEDILERALEFMTTSLEAAIGSH-AV 109
           FN+F  + G FKESLAED RG L LYEA+H+    EDI++ AL F    LE+   +H A 
Sbjct: 176 FNRFKGEDGRFKESLAEDIRGMLQLYEAAHLGTPSEDIMDEALSFTRYRLESLTSNHTAT 235

Query: 110 GRCGFAAQVRYALKWPLMKAMPRLAARRYISFYEKDPLHDSCFPSPSLIIIACKSFTTKN 169
                +  ++ AL       +  L AR YISFYE++  HD               F   N
Sbjct: 236 ASPHLSKHIQNALYRARYHNLEILVAREYISFYEQEEDHDE----------TLLKFAKLN 295

Query: 170 FMKSQEKEAIQELQKQVTNAWKEM 193
           F   Q    IQEL K +T  WKE+
Sbjct: 296 FNYCQ-LHYIQEL-KDLTKWWKEL 307

[Close]

BLAST of Sgr012593 vs. TAIR 10
Match: AT5G23960.1 (terpene synthase 21 )

HSP 1 Score: 80.1 bits (196), Expect = 2.8e-15
Identity = 55/158 (34.81%), Postives = 82/158 (51.90%), Query Frame = 0


Query: 49  IFNKFTDDKGNFKESLAEDARGTLSLYEASHIWVHGEDILERALEFMTTSLEAAIGSHAV 108
           +F KF D+ G FK  L  DA G LSLYEA+    HGEDI++ AL F  + LE  I S + 
Sbjct: 124 VFEKFKDENGKFKGHLVTDAYGMLSLYEAAQWGTHGEDIIDEALAFSRSHLE-EISSRSS 183

Query: 109 GRCGFAAQVRYALKWPLMKAMPRLAARRYISFYEKDPLHDSCFPSPSLIIIACKSFTTKN 168
                A +++ ALK P  K + R+  R+YIS+YE++   +SC   P+L+  A   F    
Sbjct: 184 PH--LAIRIKNALKHPYHKGISRIETRQYISYYEEE---ESC--DPTLLEFAKIDFNLLQ 243

Query: 169 FMKSQEKEAI------QELQKQVTNAWKEMIEDYVQSI 201
            +  +E   +       E + +VT     + E Y+ S+
Sbjct: 244 ILHREELACVTRWHHEMEFKSKVTYTRHRITEAYLWSL 273

[Close]

BLAST of Sgr012593 vs. TAIR 10
Match: AT4G13280.1 (terpenoid synthase 12 )

HSP 1 Score: 73.9 bits (180), Expect = 2.0e-13
Identity = 42/102 (41.18%), Postives = 61/102 (59.80%), Query Frame = 0


Query: 49  IFNKFTDDKGNFKESLAEDARGTLSLYEASHIWVHGEDILERALEFMTTSLEAAIGSHAV 108
           +F +FT D G F++SL EDA+G LSLYEA+H+    + IL+ AL+F ++ L++ +   A 
Sbjct: 128 VFKRFTGDDGKFQQSLTEDAKGILSLYEAAHLGTTTDYILDEALKFTSSHLKSLL---AG 187

Query: 109 GRC--GFAAQVRYALKWPLMKAMPRLAARRYISFYEKDPLHD 149
           G C       +R  L  P    M  + AR YISFYE++  HD
Sbjct: 188 GTCRPHILRLIRNTLYLPQRWNMEAVIAREYISFYEQEEDHD 226

[Close]

BLAST of Sgr012593 vs. TAIR 10
Match: AT4G13300.1 (terpenoid synthase 13 )

HSP 1 Score: 73.6 bits (179), Expect = 2.6e-13
Identity = 41/100 (41.00%), Postives = 59/100 (59.00%), Query Frame = 0


Query: 49  IFNKFTDDKGNFKESLAEDARGTLSLYEASHIWVHGEDILERALEFMTTSLEAAIGSHAV 108
           +F +FT D G F+ SL EDA+G LSLYEA+H+    + IL+ ALEF ++ L++ +    +
Sbjct: 128 VFKRFTGDDGKFERSLTEDAKGILSLYEAAHLGTTTDYILDEALEFTSSHLKSLLVG-GM 187

Query: 109 GRCGFAAQVRYALKWPLMKAMPRLAARRYISFYEKDPLHD 149
            R      +R  L  P    M  + AR YISFYE++  HD
Sbjct: 188 CRPHILRLIRNTLYLPQRWNMEAVIAREYISFYEQEEDHD 226

The following BLAST results are available for this feature:

BLAST of Sgr012593 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022154020.1	1.2e-47	38.01	(+)-gamma-cadinene synthase-like [Momordica charantia]	[more]
XP_022154019.1	1.3e-43	33.87	(-)-germacrene D synthase-like [Momordica charantia]	[more]
XP_022154012.1	4.2e-34	55.77	(-)-germacrene D synthase-like [Momordica charantia]	[more]
XP_038905736.1	3.0e-32	29.72	(-)-germacrene D synthase-like [Benincasa hispida]	[more]
XP_008448865.1	9.8e-31	29.25	PREDICTED: (-)-germacrene D synthase-like [Cucumis melo]	[more]

BLAST of Sgr012593 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B2KSJ5	2.3e-22	50.46	(+)-gamma-cadinene synthase OS=Cucumis melo OX=3656 PE=1 SV=1	[more]
Q6Q3H2	3.0e-22	43.14	Valencene synthase OS=Vitis vinifera OX=29760 GN=ValCS PE=1 SV=1	[more]
Q6Q3H3	2.5e-21	50.46	(-)-germacrene D synthase OS=Vitis vinifera OX=29760 GN=VIT_19s0014g04930 PE=1 S...	[more]
Q94JS8	1.2e-20	45.95	(E)-beta-farnesene synthase OS=Citrus junos OX=135197 PE=1 SV=1	[more]
K4L9M2	4.7e-20	50.00	Sesquiterpene synthase 1 OS=Thapsia garganica OX=79022 GN=STS1 PE=1 SV=1	[more]

BLAST of Sgr012593 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DIG3	5.6e-48	38.01	(+)-gamma-cadinene synthase-like OS=Momordica charantia OX=3673 GN=LOC111021378 ...	[more]
A0A6J1DKT6	6.4e-44	33.87	(-)-germacrene D synthase-like OS=Momordica charantia OX=3673 GN=LOC111021377 PE...	[more]
A0A6J1DKI4	2.1e-34	55.77	(-)-germacrene D synthase-like OS=Momordica charantia OX=3673 GN=LOC111021373 PE...	[more]
A0A1S3BLL1	4.7e-31	29.25	(-)-germacrene D synthase-like OS=Cucumis melo OX=3656 GN=LOC103490898 PE=4 SV=1	[more]
A0A6J1CQG9	4.0e-30	29.26	(-)-germacrene D synthase-like OS=Momordica charantia OX=3673 GN=LOC111013811 PE...	[more]

BLAST of Sgr012593 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G23960.2	3.3e-16	29.49	terpene synthase 21	[more]
AT3G14490.1	1.2e-15	38.89	Terpenoid cyclases/Protein prenyltransferases superfamily protein	[more]
AT5G23960.1	2.8e-15	34.81	terpene synthase 21	[more]
AT4G13280.1	2.0e-13	41.18	terpenoid synthase 12	[more]
AT4G13300.1	2.6e-13	41.00	terpenoid synthase 13	[more]

InterPro

Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036965	Terpene synthase, N-terminal domain superfamily	GENE3D	1.50.10.130		coord: 36..163 e-value: 6.9E-30 score: 106.0
IPR001906	Terpene synthase, N-terminal domain	PFAM	PF01397	Terpene_synth	coord: 45..121 e-value: 1.7E-17 score: 63.8
IPR008949	Isoprenoid synthase domain superfamily	GENE3D	1.10.600.10	Farnesyl Diphosphate Synthase	coord: 164..248 e-value: 1.3E-16 score: 62.3
IPR008949	Isoprenoid synthase domain superfamily	SUPERFAMILY	48576	Terpenoid synthases	coord: 171..246
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 8..27
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..27
None	No IPR available	PANTHER	PTHR31225:SF111	TERPENE SYNTHASE 2-RELATED	coord: 45..149
None	No IPR available	PANTHER	PTHR31225	OS04G0344100 PROTEIN-RELATED	coord: 173..233 coord: 45..149
None	No IPR available	PANTHER	PTHR31225:SF111	TERPENE SYNTHASE 2-RELATED	coord: 173..233
IPR008930	Terpenoid cyclases/protein prenyltransferase alpha-alpha toroid	SUPERFAMILY	48239	Terpenoid cyclases/Protein prenyltransferases	coord: 45..145

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sgr012593.1	Sgr012593.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0010333	terpene synthase activity
molecular_function	GO:0016829	lyase activity