Sgr012141 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr012141
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
DescriptionRING-type E3 ubiquitin transferase
Locationtig00153234: 93545 .. 96023 (+)
RNA-Seq ExpressionSgr012141
SyntenySgr012141
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTCCTGGAAGCAGTGCTTGCAAAGAAGTTATTGATTTTCATCCTTCAGTTTCAGGTCATGACATAGCAATGTCTAAATCGGAGAGTTCATCTACATCTACTTCTACATCTACGAAAGTTCTACTTGGTTTGGGTGGGAAACTTGGAGTTTATTCAACCAATGGAGTGCAGGAATTGCTTGAGTGTCCTGTCTGCACAAATCTGATGTACCCTCCAATTTATCAGGTATGTTCTTTATATCCTTATTATCTTTTCTCTCTTATGACTTAGACTGTTTTTGTAAGCTTAAGCAAAGCCGGTGCGGGTCACATAAAGTTGCCTAGGCCTGTGTAATACTGGCGAAATGCTTTAGCAAATTACCTCCATTTGCAGAGCATCTTTCATATTATGCCCAGTGTGCTTTAGAGTCATTTCAGTGCTTGGGCCCAATAAATGAAAGATGCTGTTAATTTCTTAAGGTTGATTGGAGTATGCAGAATTTTATTAAGAATCTTCGTAAGTTGCTGAATTCATTATTATGCAGATTTTTTAGAATGTTGCCATAGAAACATGAAGGCTGGTGCATGCATATTAGAATGGTGTTTACCTTCCTATGAGCTTCTTATTTAAGCAATAAAAAATTTATTCTAAAGTTTGACAATGTAGGGCCAATTTGGTTCATTCCCCTTTTTTTACTTCTTGTTTCTCATTTTAGTTTGGAGCAACGTGTTCTTGTCCAACATGAACACTCAAACACTTGTTGAGCACGTATTGAACACTTGTTAAGCACAATAGACACGTGTCAAACACTAGTTGGACAAATTGAATATGAGTACGACATTTGTTAGATACGTGTCAAACATTAGTTTAGTACATAACTACGTGTTGAAGACTTGTGGATATATGACAACACATGAACTTAACTGTGATGCATCTTATTTTGTTGAGAAGATAAACTATGGATTTCTGCAGTTTTTAAGCCGCACTTTATGAATTTGAATGGCCTAATAGCTTGATTGTTGATTTGATCTTCACTGGAACTGGAAGGTTGATTTGGACTGTGAACTTTGGCAAATTTTCCCCCTTGCTGGTTCTTAACTTCTTGTCAATATTAAAGTTGCACTGAAAACTTAGAACACCTCTTTATTCTTAAATCTTTATGACATGGAGGAATGATATATTTTTCTGCTTCTGCTTTGTTTCGGACTTGCTCGACCAACTTTCGAACTTTAGTGCCCAAATGGCCACACATTGTGTTCAAACTGCAAGATCAGAGTTAGCAACATTTGCCCATCCTGTCGTCATGAACTCGGCAATATAAGGTGCTTGGCGTTGGAGAAGGTAGCTGAATCGCTTGAGATCCCCTGCAGATACCAGAGTTTGGGTTGTCAAGATATTTTTCCTTACTATAGTAAGCTTAAGCATGAGCAACACTGTCGATTCCGTCCTTACAGCTGCCCTTATGCTGGATCCGAGTGCTCGGTCACAGGCGACATCCCGTCCCTTGTCGCACATCTCAAAGATGATCACAAGGTTGATATGCATGATGGGTGTACCTTCAATCATCGCTATGTGAAATCAAATCCACACGAAGTTGAAAATGCCACATGGATGCTAACTGTATGTGTTCGCTCTATATAACATCAATTTATCTTCTCACTCCATTGATTTTATATAACATTAACTTTTTTGTTTGGGTAAATTGTACGAACCACACATGAACTATTGTGATGGTTACAATTACGCCACAAACTTTTAATTGTAAAAATGTCTCTCATCGTCTCTCTTACAAGGGTTATATCTTAGATCTTCTAACAAGGAACGATAGACTTTTTTAGAGAAAGGAATGTGTAAGAGAGAGAGAACATTTTTAGAGAGAGGAGTGTGTTAGGAGATTTTTTAGAGAGAGGAGTGTGGTAAAGAGAGAGTATTTTAAAGTGTGGCAGAGAGCGAAAGAGACGTTTTTTAAGAAAAGAAAATTGAAACTACTTTCTTATCTTTCATCAGACAAAAGTCTTAAATGGGGTAACGGTAAAATCTATTTTTCACTTAAAGGTTTAATTTTTATTGGTCCTGGTTTGTGCTCTTTTTTTCTTTCTAGATGAAGATGTGTGATAAACTTAGTGTTCTTCCATCTTTGAATATTGGAATGTTGTTTTATACCTTGCACTGGCAGGTTTTCAACTGTTTTGGAAGGCAGTTTTGCTTGCATTTCGAGGCGTTCCAGCTCGGAATGGCGCCTGTTTACATGGCGTTTTTACGGTTCATGGGCGACGATAACGAAGCAAAAAAGTTCAGCTACAGTTTGGAAGTGGGTGGCAACGGCCGTAAACTGATATGGCAAGGAATTCCGAGGGGCATCCGCGACAGTCACCGAAAAGTTCGCGACAGTCAAGACGGTCTCATCATTCAAAGAAACCTGGCACTGTATTTTTCGGGAGGGGAAAGGCAAGAGCTGAAGCTGAGAATCACAGGTCGCATATGGAAAGAAGAATGA

mRNA sequence

ATGGCTCCTGGAAGCAGTGCTTGCAAAGAAGTTATTGATTTTCATCCTTCAGTTTCAGGTCATGACATAGCAATGTCTAAATCGGAGAGTTCATCTACATCTACTTCTACATCTACGAAAGTTCTACTTGGTTTGGGTGGGAAACTTGGAGTTTATTCAACCAATGGAGTGCAGGAATTGCTTGAGTGTCCTGTCTGCACAAATCTGATGTACCCTCCAATTTATCAGTGCCCAAATGGCCACACATTGTGTTCAAACTGCAAGATCAGAGTTAGCAACATTTGCCCATCCTGTCGTCATGAACTCGGCAATATAAGGTGCTTGGCGTTGGAGAAGGTAGCTGAATCGCTTGAGATCCCCTGCAGATACCAGAGTTTGGGTTGTCAAGATATTTTTCCTTACTATAGTAAGCTTAAGCATGAGCAACACTGTCGATTCCGTCCTTACAGCTGCCCTTATGCTGGATCCGAGTGCTCGGTCACAGGCGACATCCCGTCCCTTGTCGCACATCTCAAAGATGATCACAAGGTTGATATGCATGATGGGTGTACCTTCAATCATCGCTATGTGAAATCAAATCCACACGAAGTTGAAAATGCCACATGGATGCTAACTGTTTTCAACTGTTTTGGAAGGCAGTTTTGCTTGCATTTCGAGGCGTTCCAGCTCGGAATGGCGCCTGTTTACATGGCGTTTTTACGGTTCATGGGCGACGATAACGAAGCAAAAAAGTTCAGCTACAGTTTGGAAGTGGGTGGCAACGGCCGTAAACTGATATGGCAAGGAATTCCGAGGGGCATCCGCGACAGTCACCGAAAAGTTCGCGACAGTCAAGACGGTCTCATCATTCAAAGAAACCTGGCACTGTATTTTTCGGGAGGGGAAAGGCAAGAGCTGAAGCTGAGAATCACAGGTCGCATATGGAAAGAAGAATGA

Coding sequence (CDS)

ATGGCTCCTGGAAGCAGTGCTTGCAAAGAAGTTATTGATTTTCATCCTTCAGTTTCAGGTCATGACATAGCAATGTCTAAATCGGAGAGTTCATCTACATCTACTTCTACATCTACGAAAGTTCTACTTGGTTTGGGTGGGAAACTTGGAGTTTATTCAACCAATGGAGTGCAGGAATTGCTTGAGTGTCCTGTCTGCACAAATCTGATGTACCCTCCAATTTATCAGTGCCCAAATGGCCACACATTGTGTTCAAACTGCAAGATCAGAGTTAGCAACATTTGCCCATCCTGTCGTCATGAACTCGGCAATATAAGGTGCTTGGCGTTGGAGAAGGTAGCTGAATCGCTTGAGATCCCCTGCAGATACCAGAGTTTGGGTTGTCAAGATATTTTTCCTTACTATAGTAAGCTTAAGCATGAGCAACACTGTCGATTCCGTCCTTACAGCTGCCCTTATGCTGGATCCGAGTGCTCGGTCACAGGCGACATCCCGTCCCTTGTCGCACATCTCAAAGATGATCACAAGGTTGATATGCATGATGGGTGTACCTTCAATCATCGCTATGTGAAATCAAATCCACACGAAGTTGAAAATGCCACATGGATGCTAACTGTTTTCAACTGTTTTGGAAGGCAGTTTTGCTTGCATTTCGAGGCGTTCCAGCTCGGAATGGCGCCTGTTTACATGGCGTTTTTACGGTTCATGGGCGACGATAACGAAGCAAAAAAGTTCAGCTACAGTTTGGAAGTGGGTGGCAACGGCCGTAAACTGATATGGCAAGGAATTCCGAGGGGCATCCGCGACAGTCACCGAAAAGTTCGCGACAGTCAAGACGGTCTCATCATTCAAAGAAACCTGGCACTGTATTTTTCGGGAGGGGAAAGGCAAGAGCTGAAGCTGAGAATCACAGGTCGCATATGGAAAGAAGAATGA

Protein sequence

MAPGSSACKEVIDFHPSVSGHDIAMSKSESSSTSTSTSTKVLLGLGGKLGVYSTNGVQELLECPVCTNLMYPPIYQCPNGHTLCSNCKIRVSNICPSCRHELGNIRCLALEKVAESLEIPCRYQSLGCQDIFPYYSKLKHEQHCRFRPYSCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDDNEAKKFSYSLEVGGNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQELKLRITGRIWKEE
Homology
BLAST of Sgr012141 vs. NCBI nr
Match: XP_022154920.1 (E3 ubiquitin-protein ligase SINAT2-like [Momordica charantia] >XP_022154921.1 E3 ubiquitin-protein ligase SINAT2-like [Momordica charantia])

HSP 1 Score: 625.9 bits (1613), Expect = 1.8e-175
Identity = 295/315 (93.65%), Postives = 302/315 (95.87%), Query Frame = 0

Query: 1   MAPGSSACKEVIDFHPSVSGHDIAMSKSE----SSSTSTSTSTKVLLGLGGKLGVYSTNG 60
           MAPG+SACKEV+D HPSVSGHDIA+SKSE    S+S S S STKVLLGLGGKLGVYSTNG
Sbjct: 1   MAPGTSACKEVVDSHPSVSGHDIAVSKSESNSNSNSNSNSASTKVLLGLGGKLGVYSTNG 60

Query: 61  VQELLECPVCTNLMYPPIYQCPNGHTLCSNCKIRVSNICPSCRHELGNIRCLALEKVAES 120
           VQELLECPVCTNLMYPPIYQCPNGHTLCSNCKIRVSN CP+CRHELGNIRCLALEKVAES
Sbjct: 61  VQELLECPVCTNLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAES 120

Query: 121 LEIPCRYQSLGCQDIFPYYSKLKHEQHCRFRPYSCPYAGSECSVTGDIPSLVAHLKDDHK 180
           LEIPCRY SLGC DIFPYYSKLKHEQHCRFRPY+CPYAGSECSV+GDIPSLV HLKDDHK
Sbjct: 121 LEIPCRYHSLGCLDIFPYYSKLKHEQHCRFRPYNCPYAGSECSVSGDIPSLVTHLKDDHK 180

Query: 181 VDMHDGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFM 240
           VDMHDGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFM
Sbjct: 181 VDMHDGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFM 240

Query: 241 GDDNEAKKFSYSLEVGGNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGER 300
           GDDNEAKKFSYSLEVGGNGRKLIW GIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGER
Sbjct: 241 GDDNEAKKFSYSLEVGGNGRKLIWHGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGER 300

Query: 301 QELKLRITGRIWKEE 312
           QELKLRITGRIWKEE
Sbjct: 301 QELKLRITGRIWKEE 315

BLAST of Sgr012141 vs. NCBI nr
Match: XP_022928730.1 (E3 ubiquitin-protein ligase SINAT2-like isoform X1 [Cucurbita moschata] >XP_022928731.1 E3 ubiquitin-protein ligase SINAT2-like isoform X1 [Cucurbita moschata] >XP_022928732.1 E3 ubiquitin-protein ligase SINAT2-like isoform X1 [Cucurbita moschata] >XP_022928733.1 E3 ubiquitin-protein ligase SINAT2-like isoform X1 [Cucurbita moschata] >XP_022928734.1 E3 ubiquitin-protein ligase SINAT2-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 620.9 bits (1600), Expect = 5.8e-174
Identity = 292/311 (93.89%), Postives = 300/311 (96.46%), Query Frame = 0

Query: 1   MAPGSSACKEVIDFHPSVSGHDIAMSKSESSSTSTSTSTKVLLGLGGKLGVYSTNGVQEL 60
           MAPGSS CKEV+D HPSVSGHDIAMSKSE++    STS+KVLLG  GKLGVYSTNGVQEL
Sbjct: 3   MAPGSSTCKEVVDSHPSVSGHDIAMSKSENN----STSSKVLLGSSGKLGVYSTNGVQEL 62

Query: 61  LECPVCTNLMYPPIYQCPNGHTLCSNCKIRVSNICPSCRHELGNIRCLALEKVAESLEIP 120
           LECPVCTNLMYPPIYQCPNGHTLCSNCKIRVSN CP+CRHELGNIRCLALEKVAESLEIP
Sbjct: 63  LECPVCTNLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIP 122

Query: 121 CRYQSLGCQDIFPYYSKLKHEQHCRFRPYSCPYAGSECSVTGDIPSLVAHLKDDHKVDMH 180
           CRYQ+LGCQDIFPYYSKLKHEQHCRFRPY+CPYAGSECSVTGDIPSLVAHLKDDHKVDMH
Sbjct: 123 CRYQNLGCQDIFPYYSKLKHEQHCRFRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMH 182

Query: 181 DGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDDN 240
           DGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLG APVYMAFLRFMG+DN
Sbjct: 183 DGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGTAPVYMAFLRFMGEDN 242

Query: 241 EAKKFSYSLEVGGNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQELK 300
           EAKKFSYSLEVG NGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQELK
Sbjct: 243 EAKKFSYSLEVGHNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQELK 302

Query: 301 LRITGRIWKEE 312
           LRITGRIWKEE
Sbjct: 303 LRITGRIWKEE 309

BLAST of Sgr012141 vs. NCBI nr
Match: XP_038881778.1 (E3 ubiquitin-protein ligase SINAT2-like [Benincasa hispida] >XP_038881779.1 E3 ubiquitin-protein ligase SINAT2-like [Benincasa hispida])

HSP 1 Score: 620.5 bits (1599), Expect = 7.6e-174
Identity = 289/311 (92.93%), Postives = 301/311 (96.78%), Query Frame = 0

Query: 1   MAPGSSACKEVIDFHPSVSGHDIAMSKSESSSTSTSTSTKVLLGLGGKLGVYSTNGVQEL 60
           MAPGSS CKEV+D HPSVSGHDIAMSK+E++    STS+K  LGL GK+GVYSTNGVQEL
Sbjct: 1   MAPGSSTCKEVVDSHPSVSGHDIAMSKTENN----STSSKAALGLSGKIGVYSTNGVQEL 60

Query: 61  LECPVCTNLMYPPIYQCPNGHTLCSNCKIRVSNICPSCRHELGNIRCLALEKVAESLEIP 120
           LECPVCTNLMYPPIYQCP+GHTLCSNCKIRVSN CP+CRHELGNIRCLALEKVAESLEIP
Sbjct: 61  LECPVCTNLMYPPIYQCPSGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIP 120

Query: 121 CRYQSLGCQDIFPYYSKLKHEQHCRFRPYSCPYAGSECSVTGDIPSLVAHLKDDHKVDMH 180
           CRYQ+LGCQDIFPYYSKLKHEQHCR+RPY+CPYAGSECSVTGDIPSLVAHLKDDHKVDMH
Sbjct: 121 CRYQNLGCQDIFPYYSKLKHEQHCRYRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMH 180

Query: 181 DGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDDN 240
           DGCTFNHRYVKSNPHEVENATWMLTVFNCFG+QFCLHFEAFQLGMAPVYMAFLRFMGDDN
Sbjct: 181 DGCTFNHRYVKSNPHEVENATWMLTVFNCFGKQFCLHFEAFQLGMAPVYMAFLRFMGDDN 240

Query: 241 EAKKFSYSLEVGGNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQELK 300
           EAKKFSYSLEVGGNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQELK
Sbjct: 241 EAKKFSYSLEVGGNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQELK 300

Query: 301 LRITGRIWKEE 312
           LRITGRIWKEE
Sbjct: 301 LRITGRIWKEE 307

BLAST of Sgr012141 vs. NCBI nr
Match: XP_022967856.1 (E3 ubiquitin-protein ligase SINAT2-like isoform X1 [Cucurbita maxima] >XP_023543585.1 E3 ubiquitin-protein ligase SINAT2-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023543586.1 E3 ubiquitin-protein ligase SINAT2-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023543587.1 E3 ubiquitin-protein ligase SINAT2-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 619.4 bits (1596), Expect = 1.7e-173
Identity = 291/311 (93.57%), Postives = 300/311 (96.46%), Query Frame = 0

Query: 1   MAPGSSACKEVIDFHPSVSGHDIAMSKSESSSTSTSTSTKVLLGLGGKLGVYSTNGVQEL 60
           MAPGSS CKEV+D HPSVSGHDIAMSKSE++    STS+KVLLG  GKLGVYSTNGVQEL
Sbjct: 3   MAPGSSTCKEVVDSHPSVSGHDIAMSKSENN----STSSKVLLGSSGKLGVYSTNGVQEL 62

Query: 61  LECPVCTNLMYPPIYQCPNGHTLCSNCKIRVSNICPSCRHELGNIRCLALEKVAESLEIP 120
           LECPVCTNLMYPPIYQCPNGHTLCSNCKIRVSN CP+CRHELGNIRCLALEKVAESLEIP
Sbjct: 63  LECPVCTNLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIP 122

Query: 121 CRYQSLGCQDIFPYYSKLKHEQHCRFRPYSCPYAGSECSVTGDIPSLVAHLKDDHKVDMH 180
           CRYQ+LGCQDIFPYYSKLKHEQHCRFRPY+CPYAGSECSVTGDIPSLVAHLKDDHKVDMH
Sbjct: 123 CRYQNLGCQDIFPYYSKLKHEQHCRFRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMH 182

Query: 181 DGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDDN 240
           DGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLG APVYMAFLRFMG+DN
Sbjct: 183 DGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGTAPVYMAFLRFMGEDN 242

Query: 241 EAKKFSYSLEVGGNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQELK 300
           EAKKFSYSLEVG NGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGER+ELK
Sbjct: 243 EAKKFSYSLEVGHNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERRELK 302

Query: 301 LRITGRIWKEE 312
           LRITGRIWKEE
Sbjct: 303 LRITGRIWKEE 309

BLAST of Sgr012141 vs. NCBI nr
Match: KAG6603259.1 (E3 ubiquitin-protein ligase SINAT2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7033559.1 E3 ubiquitin-protein ligase SINAT2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 617.1 bits (1590), Expect = 8.4e-173
Identity = 290/311 (93.25%), Postives = 299/311 (96.14%), Query Frame = 0

Query: 1   MAPGSSACKEVIDFHPSVSGHDIAMSKSESSSTSTSTSTKVLLGLGGKLGVYSTNGVQEL 60
           MAPGSS CKEV+D HPSVSGHDIAMSKSE++    STS+KVLLG  GKLGVYSTNGVQEL
Sbjct: 3   MAPGSSTCKEVVDSHPSVSGHDIAMSKSENN----STSSKVLLGSSGKLGVYSTNGVQEL 62

Query: 61  LECPVCTNLMYPPIYQCPNGHTLCSNCKIRVSNICPSCRHELGNIRCLALEKVAESLEIP 120
           LECPVC NLMYPPIYQCPNGHTLCSNCKIRVSN CP+CRHELGNIRCLALEKVAESLEIP
Sbjct: 63  LECPVCMNLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIP 122

Query: 121 CRYQSLGCQDIFPYYSKLKHEQHCRFRPYSCPYAGSECSVTGDIPSLVAHLKDDHKVDMH 180
           CRYQ+LGCQDIFPYYSKLKHEQHCRFRPY+CPYAGSECSVTGDIPSLVAHLKDDHKVDMH
Sbjct: 123 CRYQNLGCQDIFPYYSKLKHEQHCRFRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMH 182

Query: 181 DGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDDN 240
           DGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLG APVYMAFLRFMG+DN
Sbjct: 183 DGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGTAPVYMAFLRFMGEDN 242

Query: 241 EAKKFSYSLEVGGNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQELK 300
           EAKKFSYSLEVG NGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGER+ELK
Sbjct: 243 EAKKFSYSLEVGHNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERRELK 302

Query: 301 LRITGRIWKEE 312
           LRITGRIWKEE
Sbjct: 303 LRITGRIWKEE 309

BLAST of Sgr012141 vs. ExPASy Swiss-Prot
Match: Q9M2P4 (E3 ubiquitin-protein ligase SINAT2 OS=Arabidopsis thaliana OX=3702 GN=SINAT2 PE=1 SV=1)

HSP 1 Score: 545.4 bits (1404), Expect = 4.1e-154
Identity = 253/311 (81.35%), Postives = 277/311 (89.07%), Query Frame = 0

Query: 1   MAPGSSACKEVIDFHPSVSGHDIAMSKSESSSTSTSTSTKVLLGLGGKLGVYSTNGVQEL 60
           MAPG SA KEV++ + +   +++  +K E    + +  TK      GK G++S NGV EL
Sbjct: 1   MAPGGSALKEVMESNSTGMDYEVKTAKVE---VNNNKPTKPGSAGIGKYGIHSNNGVYEL 60

Query: 61  LECPVCTNLMYPPIYQCPNGHTLCSNCKIRVSNICPSCRHELGNIRCLALEKVAESLEIP 120
           LECPVCTNLMYPPI+QCPNGHTLCSNCK+RV N CP+CR+ELGNIRCLALEKVAESLE+P
Sbjct: 61  LECPVCTNLMYPPIHQCPNGHTLCSNCKLRVQNTCPTCRYELGNIRCLALEKVAESLEVP 120

Query: 121 CRYQSLGCQDIFPYYSKLKHEQHCRFRPYSCPYAGSECSVTGDIPSLVAHLKDDHKVDMH 180
           CRYQ+LGC DIFPYYSKLKHEQHCRFRPY+CPYAGSECSVTGDIP+LV HLKDDHKVDMH
Sbjct: 121 CRYQNLGCHDIFPYYSKLKHEQHCRFRPYTCPYAGSECSVTGDIPTLVVHLKDDHKVDMH 180

Query: 181 DGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDDN 240
           DGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGD+N
Sbjct: 181 DGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDEN 240

Query: 241 EAKKFSYSLEVGGNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQELK 300
           EAKKFSYSLEVG +GRKL WQGIPR IRDSHRKVRDSQDGLII RNLALYFSGG+RQELK
Sbjct: 241 EAKKFSYSLEVGAHGRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNLALYFSGGDRQELK 300

Query: 301 LRITGRIWKEE 312
           LR+TGRIWKEE
Sbjct: 301 LRVTGRIWKEE 308

BLAST of Sgr012141 vs. ExPASy Swiss-Prot
Match: P93748 (Putative E3 ubiquitin-protein ligase SINAT1 OS=Arabidopsis thaliana OX=3702 GN=SINAT1 PE=1 SV=1)

HSP 1 Score: 534.6 bits (1376), Expect = 7.2e-151
Identity = 248/312 (79.49%), Postives = 277/312 (88.78%), Query Frame = 0

Query: 1   MAPGSSACKEVIDFHPSVSGHDIAMSKSESSSTSTSTSTKVLLGLGGKLG-VYSTNGVQE 60
           MAPG SA KE ++ + +   +++ M+K E++S  T + +       G +G  +S+NGV E
Sbjct: 1   MAPGGSALKEALESNSTGVDYEVKMAKVEANSKPTKSGS-------GSIGKFHSSNGVYE 60

Query: 61  LLECPVCTNLMYPPIYQCPNGHTLCSNCKIRVSNICPSCRHELGNIRCLALEKVAESLEI 120
           LLECPVCTNLMYPPI+QCPNGHTLCS+CK+RV N CP+CR+ELGNIRCLALEKVAESLE+
Sbjct: 61  LLECPVCTNLMYPPIHQCPNGHTLCSSCKLRVQNTCPTCRYELGNIRCLALEKVAESLEV 120

Query: 121 PCRYQSLGCQDIFPYYSKLKHEQHCRFRPYSCPYAGSECSVTGDIPSLVAHLKDDHKVDM 180
           PCRYQ+LGCQDIFPYYSKLKHEQHCRFR YSCPYAGSECSVTGDIP+LV HLKDDHKVDM
Sbjct: 121 PCRYQNLGCQDIFPYYSKLKHEQHCRFRSYSCPYAGSECSVTGDIPTLVDHLKDDHKVDM 180

Query: 181 HDGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDD 240
           HDGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGD+
Sbjct: 181 HDGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDE 240

Query: 241 NEAKKFSYSLEVGGNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQEL 300
           NEAKKFSYSLEVG + RKL WQGIPR IRDSHRKVRDSQDGLII RNLALYFSG +++EL
Sbjct: 241 NEAKKFSYSLEVGAHSRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNLALYFSGSDKEEL 300

Query: 301 KLRITGRIWKEE 312
           KLR+TGRIWKEE
Sbjct: 301 KLRVTGRIWKEE 305

BLAST of Sgr012141 vs. ExPASy Swiss-Prot
Match: Q10L91 (E3 ubiquitin-protein ligase DIS1 OS=Oryza sativa subsp. japonica OX=39947 GN=DIS1 PE=1 SV=1)

HSP 1 Score: 473.0 bits (1216), Expect = 2.6e-132
Identity = 211/264 (79.92%), Postives = 236/264 (89.39%), Query Frame = 0

Query: 48  KLGVYSTNGVQELLECPVCTNLMYPPIYQCPNGHTLCSNCKIRVSNICPSCRHELGNIRC 107
           K  V  ++ V+ELLECPVC + MYPPI+QC NGHTLCS CK RV N CP+CRHELGNIRC
Sbjct: 38  KPNVVVSSSVRELLECPVCLSAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRC 97

Query: 108 LALEKVAESLEIPCRYQSLGCQDIFPYYSKLKHEQHCRFRPYSCPYAGSECSVTGDIPSL 167
           LALEKVA SLE+PC+YQ+ GC  I+PYY KLKHE  C++RPYSCPYAGSEC+V GDIP L
Sbjct: 98  LALEKVAASLELPCKYQNFGCVGIYPYYCKLKHESQCQYRPYSCPYAGSECTVAGDIPYL 157

Query: 168 VAHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAP 227
           V HLKDDHKVDMH+GCTFNHRYVKSNPHEVENATWMLTVF+CFG+ FCLHFEAFQLGMAP
Sbjct: 158 VNHLKDDHKVDMHNGCTFNHRYVKSNPHEVENATWMLTVFSCFGQYFCLHFEAFQLGMAP 217

Query: 228 VYMAFLRFMGDDNEAKKFSYSLEVGGNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNL 287
           VY+AFLRFMGDD EAK +SYSLEVGG GRK+IWQG+PR IRDSHRKVRDS DGLIIQRN+
Sbjct: 218 VYIAFLRFMGDDLEAKNYSYSLEVGGTGRKMIWQGVPRSIRDSHRKVRDSYDGLIIQRNM 277

Query: 288 ALYFSGGERQELKLRITGRIWKEE 312
           AL+FSGGER+ELKLR+TGRIWKE+
Sbjct: 278 ALFFSGGERKELKLRVTGRIWKEQ 301

BLAST of Sgr012141 vs. ExPASy Swiss-Prot
Match: Q84JL3 (E3 ubiquitin-protein ligase SINAT3 OS=Arabidopsis thaliana OX=3702 GN=SINAT3 PE=1 SV=1)

HSP 1 Score: 471.1 bits (1211), Expect = 9.8e-132
Identity = 216/288 (75.00%), Postives = 246/288 (85.42%), Query Frame = 0

Query: 24  AMSKSESSSTSTSTSTKVLLGLGGKLGVYSTNGVQELLECPVCTNLMYPPIYQCPNGHTL 83
           ++SK   ++ +T+       GL     + +T  V ELLECPVCTN MYPPI+QC NGHTL
Sbjct: 29  SVSKHHHTNNNTTNVNAAASGL-----LPTTTSVHELLECPVCTNSMYPPIHQCHNGHTL 88

Query: 84  CSNCKIRVSNICPSCRHELGNIRCLALEKVAESLEIPCRYQSLGCQDIFPYYSKLKHEQH 143
           CS CK RV N CP+CR ELG+IRCLALEKVAESLE+PC++ SLGC +IFPYYSKLKHE  
Sbjct: 89  CSTCKARVHNRCPTCRQELGDIRCLALEKVAESLELPCKHMSLGCPEIFPYYSKLKHETV 148

Query: 144 CRFRPYSCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWM 203
           C FRPYSCPYAGSECSVTGDIP LVAHL+DDHKVDMH GCTFNHRYVKSNP EVENATWM
Sbjct: 149 CNFRPYSCPYAGSECSVTGDIPFLVAHLRDDHKVDMHSGCTFNHRYVKSNPREVENATWM 208

Query: 204 LTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDDNEAKKFSYSLEVGGNGRKLIWQGI 263
           LTVF+CFG+ FCLHFEAFQLGMAPVYMAFLRFMGD+ EA+ ++YSLEVGG GRKLIW+G 
Sbjct: 209 LTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDETEARNYNYSLEVGGYGRKLIWEGT 268

Query: 264 PRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQELKLRITGRIWKEE 312
           PR +RDSHRKVRDS DGLIIQRN+AL+FSGG+R+ELKLR+TGRIWKE+
Sbjct: 269 PRSVRDSHRKVRDSHDGLIIQRNMALFFSGGDRKELKLRVTGRIWKEQ 311

BLAST of Sgr012141 vs. ExPASy Swiss-Prot
Match: Q9STN8 (E3 ubiquitin-protein ligase SINAT4 OS=Arabidopsis thaliana OX=3702 GN=SINAT4 PE=1 SV=1)

HSP 1 Score: 457.6 bits (1176), Expect = 1.1e-127
Identity = 208/299 (69.57%), Postives = 244/299 (81.61%), Query Frame = 0

Query: 13  DFHPSVSGHDIAMSKSESSSTSTSTSTKVLLGLGGKLGVYSTNGVQELLECPVCTNLMYP 72
           + H + +GH  +   S + +   + +  V+  + G         V ELLECPVCT  MYP
Sbjct: 15  EIHQNGNGHQ-SYQFSSTKTHGGAAAAAVVTNIVGPTATAPATSVYELLECPVCTYSMYP 74

Query: 73  PIYQCPNGHTLCSNCKIRVSNICPSCRHELGNIRCLALEKVAESLEIPCRYQSLGCQDIF 132
           PI+QC NGHTLCS CK+RV N CP+CR ELG+IRCLALEKVAESLE+PC++ +LGC +IF
Sbjct: 75  PIHQCHNGHTLCSTCKVRVHNRCPTCRQELGDIRCLALEKVAESLELPCKFYNLGCPEIF 134

Query: 133 PYYSKLKHEQHCRFRPYSCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGCTFNHRYVKS 192
           PYYSKLKHE  C FRPYSCPYAGSEC + GDIP LVAHL+DDHKVDMH G TFNHRYVKS
Sbjct: 135 PYYSKLKHESLCNFRPYSCPYAGSECGIVGDIPFLVAHLRDDHKVDMHAGSTFNHRYVKS 194

Query: 193 NPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDDNEAKKFSYSLEVG 252
           NP EVENATWMLTVF+CFG+ FCLHFEAFQLGM PVYMAFLRFMGD+ +A+ +SYSLEVG
Sbjct: 195 NPREVENATWMLTVFHCFGQYFCLHFEAFQLGMGPVYMAFLRFMGDEEDARSYSYSLEVG 254

Query: 253 GNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQELKLRITGRIWKEE 312
           G+GRKL W+G PR IRDSHRKVRDS DGLIIQRN+AL+FSGG+R+ELKLR+TG+IWKE+
Sbjct: 255 GSGRKLTWEGTPRSIRDSHRKVRDSNDGLIIQRNMALFFSGGDRKELKLRVTGKIWKEQ 312

BLAST of Sgr012141 vs. ExPASy TrEMBL
Match: A0A6J1DL03 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111022066 PE=3 SV=1)

HSP 1 Score: 625.9 bits (1613), Expect = 8.8e-176
Identity = 295/315 (93.65%), Postives = 302/315 (95.87%), Query Frame = 0

Query: 1   MAPGSSACKEVIDFHPSVSGHDIAMSKSE----SSSTSTSTSTKVLLGLGGKLGVYSTNG 60
           MAPG+SACKEV+D HPSVSGHDIA+SKSE    S+S S S STKVLLGLGGKLGVYSTNG
Sbjct: 1   MAPGTSACKEVVDSHPSVSGHDIAVSKSESNSNSNSNSNSASTKVLLGLGGKLGVYSTNG 60

Query: 61  VQELLECPVCTNLMYPPIYQCPNGHTLCSNCKIRVSNICPSCRHELGNIRCLALEKVAES 120
           VQELLECPVCTNLMYPPIYQCPNGHTLCSNCKIRVSN CP+CRHELGNIRCLALEKVAES
Sbjct: 61  VQELLECPVCTNLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAES 120

Query: 121 LEIPCRYQSLGCQDIFPYYSKLKHEQHCRFRPYSCPYAGSECSVTGDIPSLVAHLKDDHK 180
           LEIPCRY SLGC DIFPYYSKLKHEQHCRFRPY+CPYAGSECSV+GDIPSLV HLKDDHK
Sbjct: 121 LEIPCRYHSLGCLDIFPYYSKLKHEQHCRFRPYNCPYAGSECSVSGDIPSLVTHLKDDHK 180

Query: 181 VDMHDGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFM 240
           VDMHDGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFM
Sbjct: 181 VDMHDGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFM 240

Query: 241 GDDNEAKKFSYSLEVGGNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGER 300
           GDDNEAKKFSYSLEVGGNGRKLIW GIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGER
Sbjct: 241 GDDNEAKKFSYSLEVGGNGRKLIWHGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGER 300

Query: 301 QELKLRITGRIWKEE 312
           QELKLRITGRIWKEE
Sbjct: 301 QELKLRITGRIWKEE 315

BLAST of Sgr012141 vs. ExPASy TrEMBL
Match: A0A6J1ESE8 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111435553 PE=3 SV=1)

HSP 1 Score: 620.9 bits (1600), Expect = 2.8e-174
Identity = 292/311 (93.89%), Postives = 300/311 (96.46%), Query Frame = 0

Query: 1   MAPGSSACKEVIDFHPSVSGHDIAMSKSESSSTSTSTSTKVLLGLGGKLGVYSTNGVQEL 60
           MAPGSS CKEV+D HPSVSGHDIAMSKSE++    STS+KVLLG  GKLGVYSTNGVQEL
Sbjct: 3   MAPGSSTCKEVVDSHPSVSGHDIAMSKSENN----STSSKVLLGSSGKLGVYSTNGVQEL 62

Query: 61  LECPVCTNLMYPPIYQCPNGHTLCSNCKIRVSNICPSCRHELGNIRCLALEKVAESLEIP 120
           LECPVCTNLMYPPIYQCPNGHTLCSNCKIRVSN CP+CRHELGNIRCLALEKVAESLEIP
Sbjct: 63  LECPVCTNLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIP 122

Query: 121 CRYQSLGCQDIFPYYSKLKHEQHCRFRPYSCPYAGSECSVTGDIPSLVAHLKDDHKVDMH 180
           CRYQ+LGCQDIFPYYSKLKHEQHCRFRPY+CPYAGSECSVTGDIPSLVAHLKDDHKVDMH
Sbjct: 123 CRYQNLGCQDIFPYYSKLKHEQHCRFRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMH 182

Query: 181 DGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDDN 240
           DGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLG APVYMAFLRFMG+DN
Sbjct: 183 DGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGTAPVYMAFLRFMGEDN 242

Query: 241 EAKKFSYSLEVGGNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQELK 300
           EAKKFSYSLEVG NGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQELK
Sbjct: 243 EAKKFSYSLEVGHNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQELK 302

Query: 301 LRITGRIWKEE 312
           LRITGRIWKEE
Sbjct: 303 LRITGRIWKEE 309

BLAST of Sgr012141 vs. ExPASy TrEMBL
Match: A0A6J1HT85 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111467239 PE=3 SV=1)

HSP 1 Score: 619.4 bits (1596), Expect = 8.2e-174
Identity = 291/311 (93.57%), Postives = 300/311 (96.46%), Query Frame = 0

Query: 1   MAPGSSACKEVIDFHPSVSGHDIAMSKSESSSTSTSTSTKVLLGLGGKLGVYSTNGVQEL 60
           MAPGSS CKEV+D HPSVSGHDIAMSKSE++    STS+KVLLG  GKLGVYSTNGVQEL
Sbjct: 3   MAPGSSTCKEVVDSHPSVSGHDIAMSKSENN----STSSKVLLGSSGKLGVYSTNGVQEL 62

Query: 61  LECPVCTNLMYPPIYQCPNGHTLCSNCKIRVSNICPSCRHELGNIRCLALEKVAESLEIP 120
           LECPVCTNLMYPPIYQCPNGHTLCSNCKIRVSN CP+CRHELGNIRCLALEKVAESLEIP
Sbjct: 63  LECPVCTNLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIP 122

Query: 121 CRYQSLGCQDIFPYYSKLKHEQHCRFRPYSCPYAGSECSVTGDIPSLVAHLKDDHKVDMH 180
           CRYQ+LGCQDIFPYYSKLKHEQHCRFRPY+CPYAGSECSVTGDIPSLVAHLKDDHKVDMH
Sbjct: 123 CRYQNLGCQDIFPYYSKLKHEQHCRFRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMH 182

Query: 181 DGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDDN 240
           DGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLG APVYMAFLRFMG+DN
Sbjct: 183 DGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGTAPVYMAFLRFMGEDN 242

Query: 241 EAKKFSYSLEVGGNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQELK 300
           EAKKFSYSLEVG NGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGER+ELK
Sbjct: 243 EAKKFSYSLEVGHNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERRELK 302

Query: 301 LRITGRIWKEE 312
           LRITGRIWKEE
Sbjct: 303 LRITGRIWKEE 309

BLAST of Sgr012141 vs. ExPASy TrEMBL
Match: A0A6J1EBG4 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111432512 PE=3 SV=1)

HSP 1 Score: 612.5 bits (1578), Expect = 1.0e-171
Identity = 289/311 (92.93%), Postives = 299/311 (96.14%), Query Frame = 0

Query: 1   MAPGSSACKEVIDFHPSVSGHDIAMSKSESSSTSTSTSTKVLLGLGGKLGVYSTNGVQEL 60
           MAPGSS CK+V D HPS+SGHDIA+SKSE++ T++  S    LGLGGKLGVYSTNGVQEL
Sbjct: 1   MAPGSSTCKKV-DSHPSISGHDIAISKSENNPTTSKAS----LGLGGKLGVYSTNGVQEL 60

Query: 61  LECPVCTNLMYPPIYQCPNGHTLCSNCKIRVSNICPSCRHELGNIRCLALEKVAESLEIP 120
           LECPVCTNLMYPPIYQCPNGHTLCSNCKIRVSN CP+CRHELGNIRCLALEKVAESLEIP
Sbjct: 61  LECPVCTNLMYPPIYQCPNGHTLCSNCKIRVSNSCPTCRHELGNIRCLALEKVAESLEIP 120

Query: 121 CRYQSLGCQDIFPYYSKLKHEQHCRFRPYSCPYAGSECSVTGDIPSLVAHLKDDHKVDMH 180
           CRYQ+LGCQDIFPYYSKLKHEQHCRFRPY+CPYAGSECSVTGDI SLVAHLKDDHKVDMH
Sbjct: 121 CRYQNLGCQDIFPYYSKLKHEQHCRFRPYNCPYAGSECSVTGDIQSLVAHLKDDHKVDMH 180

Query: 181 DGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDDN 240
           DGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDDN
Sbjct: 181 DGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDDN 240

Query: 241 EAKKFSYSLEVGGNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQELK 300
           EAKKFSYSLEVGGNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQELK
Sbjct: 241 EAKKFSYSLEVGGNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQELK 300

Query: 301 LRITGRIWKEE 312
           LRITGRIWKEE
Sbjct: 301 LRITGRIWKEE 306

BLAST of Sgr012141 vs. ExPASy TrEMBL
Match: A0A6J1HS09 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111466063 PE=3 SV=1)

HSP 1 Score: 609.0 bits (1569), Expect = 1.1e-170
Identity = 289/311 (92.93%), Postives = 298/311 (95.82%), Query Frame = 0

Query: 1   MAPGSSACKEVIDFHPSVSGHDIAMSKSESSSTSTSTSTKVLLGLGGKLGVYSTNGVQEL 60
           MAPGSS CK+V D H SVSGHDIA+SKSE++ T++  S    LGLGGKLGVYSTNGVQEL
Sbjct: 1   MAPGSSTCKKV-DSHLSVSGHDIAISKSENNPTTSKAS----LGLGGKLGVYSTNGVQEL 60

Query: 61  LECPVCTNLMYPPIYQCPNGHTLCSNCKIRVSNICPSCRHELGNIRCLALEKVAESLEIP 120
           LECPVCTNLMYPPIYQCPNGHTLCSNCKIRVSN CP+CRHELGNIRCLALEKVAESLEIP
Sbjct: 61  LECPVCTNLMYPPIYQCPNGHTLCSNCKIRVSNSCPTCRHELGNIRCLALEKVAESLEIP 120

Query: 121 CRYQSLGCQDIFPYYSKLKHEQHCRFRPYSCPYAGSECSVTGDIPSLVAHLKDDHKVDMH 180
           CRYQ+LGCQDIFPYYSKLKHEQHCRFRPY+CPYAGSECSVTGDI SLVAHLKDDHKVDMH
Sbjct: 121 CRYQNLGCQDIFPYYSKLKHEQHCRFRPYNCPYAGSECSVTGDIQSLVAHLKDDHKVDMH 180

Query: 181 DGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDDN 240
           DGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDDN
Sbjct: 181 DGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDDN 240

Query: 241 EAKKFSYSLEVGGNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQELK 300
           EAKKFSYSLEVGGNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQELK
Sbjct: 241 EAKKFSYSLEVGGNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQELK 300

Query: 301 LRITGRIWKEE 312
           LRITGRIWKEE
Sbjct: 301 LRITGRIWKEE 306

BLAST of Sgr012141 vs. TAIR 10
Match: AT3G58040.1 (seven in absentia of Arabidopsis 2 )

HSP 1 Score: 545.4 bits (1404), Expect = 2.9e-155
Identity = 253/311 (81.35%), Postives = 277/311 (89.07%), Query Frame = 0

Query: 1   MAPGSSACKEVIDFHPSVSGHDIAMSKSESSSTSTSTSTKVLLGLGGKLGVYSTNGVQEL 60
           MAPG SA KEV++ + +   +++  +K E    + +  TK      GK G++S NGV EL
Sbjct: 1   MAPGGSALKEVMESNSTGMDYEVKTAKVE---VNNNKPTKPGSAGIGKYGIHSNNGVYEL 60

Query: 61  LECPVCTNLMYPPIYQCPNGHTLCSNCKIRVSNICPSCRHELGNIRCLALEKVAESLEIP 120
           LECPVCTNLMYPPI+QCPNGHTLCSNCK+RV N CP+CR+ELGNIRCLALEKVAESLE+P
Sbjct: 61  LECPVCTNLMYPPIHQCPNGHTLCSNCKLRVQNTCPTCRYELGNIRCLALEKVAESLEVP 120

Query: 121 CRYQSLGCQDIFPYYSKLKHEQHCRFRPYSCPYAGSECSVTGDIPSLVAHLKDDHKVDMH 180
           CRYQ+LGC DIFPYYSKLKHEQHCRFRPY+CPYAGSECSVTGDIP+LV HLKDDHKVDMH
Sbjct: 121 CRYQNLGCHDIFPYYSKLKHEQHCRFRPYTCPYAGSECSVTGDIPTLVVHLKDDHKVDMH 180

Query: 181 DGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDDN 240
           DGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGD+N
Sbjct: 181 DGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDEN 240

Query: 241 EAKKFSYSLEVGGNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQELK 300
           EAKKFSYSLEVG +GRKL WQGIPR IRDSHRKVRDSQDGLII RNLALYFSGG+RQELK
Sbjct: 241 EAKKFSYSLEVGAHGRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNLALYFSGGDRQELK 300

Query: 301 LRITGRIWKEE 312
           LR+TGRIWKEE
Sbjct: 301 LRVTGRIWKEE 308

BLAST of Sgr012141 vs. TAIR 10
Match: AT2G41980.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 534.6 bits (1376), Expect = 5.1e-152
Identity = 248/312 (79.49%), Postives = 277/312 (88.78%), Query Frame = 0

Query: 1   MAPGSSACKEVIDFHPSVSGHDIAMSKSESSSTSTSTSTKVLLGLGGKLG-VYSTNGVQE 60
           MAPG SA KE ++ + +   +++ M+K E++S  T + +       G +G  +S+NGV E
Sbjct: 1   MAPGGSALKEALESNSTGVDYEVKMAKVEANSKPTKSGS-------GSIGKFHSSNGVYE 60

Query: 61  LLECPVCTNLMYPPIYQCPNGHTLCSNCKIRVSNICPSCRHELGNIRCLALEKVAESLEI 120
           LLECPVCTNLMYPPI+QCPNGHTLCS+CK+RV N CP+CR+ELGNIRCLALEKVAESLE+
Sbjct: 61  LLECPVCTNLMYPPIHQCPNGHTLCSSCKLRVQNTCPTCRYELGNIRCLALEKVAESLEV 120

Query: 121 PCRYQSLGCQDIFPYYSKLKHEQHCRFRPYSCPYAGSECSVTGDIPSLVAHLKDDHKVDM 180
           PCRYQ+LGCQDIFPYYSKLKHEQHCRFR YSCPYAGSECSVTGDIP+LV HLKDDHKVDM
Sbjct: 121 PCRYQNLGCQDIFPYYSKLKHEQHCRFRSYSCPYAGSECSVTGDIPTLVDHLKDDHKVDM 180

Query: 181 HDGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDD 240
           HDGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGD+
Sbjct: 181 HDGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDE 240

Query: 241 NEAKKFSYSLEVGGNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQEL 300
           NEAKKFSYSLEVG + RKL WQGIPR IRDSHRKVRDSQDGLII RNLALYFSG +++EL
Sbjct: 241 NEAKKFSYSLEVGAHSRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNLALYFSGSDKEEL 300

Query: 301 KLRITGRIWKEE 312
           KLR+TGRIWKEE
Sbjct: 301 KLRVTGRIWKEE 305

BLAST of Sgr012141 vs. TAIR 10
Match: AT3G61790.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 471.1 bits (1211), Expect = 7.0e-133
Identity = 216/288 (75.00%), Postives = 246/288 (85.42%), Query Frame = 0

Query: 24  AMSKSESSSTSTSTSTKVLLGLGGKLGVYSTNGVQELLECPVCTNLMYPPIYQCPNGHTL 83
           ++SK   ++ +T+       GL     + +T  V ELLECPVCTN MYPPI+QC NGHTL
Sbjct: 29  SVSKHHHTNNNTTNVNAAASGL-----LPTTTSVHELLECPVCTNSMYPPIHQCHNGHTL 88

Query: 84  CSNCKIRVSNICPSCRHELGNIRCLALEKVAESLEIPCRYQSLGCQDIFPYYSKLKHEQH 143
           CS CK RV N CP+CR ELG+IRCLALEKVAESLE+PC++ SLGC +IFPYYSKLKHE  
Sbjct: 89  CSTCKARVHNRCPTCRQELGDIRCLALEKVAESLELPCKHMSLGCPEIFPYYSKLKHETV 148

Query: 144 CRFRPYSCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWM 203
           C FRPYSCPYAGSECSVTGDIP LVAHL+DDHKVDMH GCTFNHRYVKSNP EVENATWM
Sbjct: 149 CNFRPYSCPYAGSECSVTGDIPFLVAHLRDDHKVDMHSGCTFNHRYVKSNPREVENATWM 208

Query: 204 LTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDDNEAKKFSYSLEVGGNGRKLIWQGI 263
           LTVF+CFG+ FCLHFEAFQLGMAPVYMAFLRFMGD+ EA+ ++YSLEVGG GRKLIW+G 
Sbjct: 209 LTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDETEARNYNYSLEVGGYGRKLIWEGT 268

Query: 264 PRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQELKLRITGRIWKEE 312
           PR +RDSHRKVRDS DGLIIQRN+AL+FSGG+R+ELKLR+TGRIWKE+
Sbjct: 269 PRSVRDSHRKVRDSHDGLIIQRNMALFFSGGDRKELKLRVTGRIWKEQ 311

BLAST of Sgr012141 vs. TAIR 10
Match: AT4G27880.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 457.6 bits (1176), Expect = 8.0e-129
Identity = 208/299 (69.57%), Postives = 244/299 (81.61%), Query Frame = 0

Query: 13  DFHPSVSGHDIAMSKSESSSTSTSTSTKVLLGLGGKLGVYSTNGVQELLECPVCTNLMYP 72
           + H + +GH  +   S + +   + +  V+  + G         V ELLECPVCT  MYP
Sbjct: 15  EIHQNGNGHQ-SYQFSSTKTHGGAAAAAVVTNIVGPTATAPATSVYELLECPVCTYSMYP 74

Query: 73  PIYQCPNGHTLCSNCKIRVSNICPSCRHELGNIRCLALEKVAESLEIPCRYQSLGCQDIF 132
           PI+QC NGHTLCS CK+RV N CP+CR ELG+IRCLALEKVAESLE+PC++ +LGC +IF
Sbjct: 75  PIHQCHNGHTLCSTCKVRVHNRCPTCRQELGDIRCLALEKVAESLELPCKFYNLGCPEIF 134

Query: 133 PYYSKLKHEQHCRFRPYSCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGCTFNHRYVKS 192
           PYYSKLKHE  C FRPYSCPYAGSEC + GDIP LVAHL+DDHKVDMH G TFNHRYVKS
Sbjct: 135 PYYSKLKHESLCNFRPYSCPYAGSECGIVGDIPFLVAHLRDDHKVDMHAGSTFNHRYVKS 194

Query: 193 NPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDDNEAKKFSYSLEVG 252
           NP EVENATWMLTVF+CFG+ FCLHFEAFQLGM PVYMAFLRFMGD+ +A+ +SYSLEVG
Sbjct: 195 NPREVENATWMLTVFHCFGQYFCLHFEAFQLGMGPVYMAFLRFMGDEEDARSYSYSLEVG 254

Query: 253 GNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQELKLRITGRIWKEE 312
           G+GRKL W+G PR IRDSHRKVRDS DGLIIQRN+AL+FSGG+R+ELKLR+TG+IWKE+
Sbjct: 255 GSGRKLTWEGTPRSIRDSHRKVRDSNDGLIIQRNMALFFSGGDRKELKLRVTGKIWKEQ 312

BLAST of Sgr012141 vs. TAIR 10
Match: AT5G53360.1 (TRAF-like superfamily protein )

HSP 1 Score: 365.9 bits (938), Expect = 3.2e-101
Identity = 161/204 (78.92%), Postives = 184/204 (90.20%), Query Frame = 0

Query: 108 LALEKVAESLEIPCRYQSLGCQDIFPYYSKLKHEQHCRFRPYSCPYAGSECSVTGDIPSL 167
           +ALEKVAESLE+PC+Y +LGC  IFPYYSKLKHE  C FRPYSCPYAGSEC+  GDI  L
Sbjct: 15  IALEKVAESLELPCKYYNLGCLGIFPYYSKLKHESQCNFRPYSCPYAGSECAAVGDITFL 74

Query: 168 VAHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAP 227
           VAHL+DDHKVDMH GCTFNHRYVKSNP EVENATWMLTVF CFG+ FCLHFEAFQLGMAP
Sbjct: 75  VAHLRDDHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFQCFGQYFCLHFEAFQLGMAP 134

Query: 228 VYMAFLRFMGDDNEAKKFSYSLEVGGNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNL 287
           VYMAFLRFMGD+++A+ ++YSLEVGG+GRK  W+G PR +RDSHRKVRDS DGLIIQRN+
Sbjct: 135 VYMAFLRFMGDEDDARNYTYSLEVGGSGRKQTWEGTPRSVRDSHRKVRDSHDGLIIQRNM 194

Query: 288 ALYFSGGERQELKLRITGRIWKEE 312
           AL+FSGG+++ELKLR+TGRIWKE+
Sbjct: 195 ALFFSGGDKKELKLRVTGRIWKEQ 218

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022154920.11.8e-17593.65E3 ubiquitin-protein ligase SINAT2-like [Momordica charantia] >XP_022154921.1 E3... [more]
XP_022928730.15.8e-17493.89E3 ubiquitin-protein ligase SINAT2-like isoform X1 [Cucurbita moschata] >XP_0229... [more]
XP_038881778.17.6e-17492.93E3 ubiquitin-protein ligase SINAT2-like [Benincasa hispida] >XP_038881779.1 E3 u... [more]
XP_022967856.11.7e-17393.57E3 ubiquitin-protein ligase SINAT2-like isoform X1 [Cucurbita maxima] >XP_023543... [more]
KAG6603259.18.4e-17393.25E3 ubiquitin-protein ligase SINAT2, partial [Cucurbita argyrosperma subsp. soror... [more]
Match NameE-valueIdentityDescription
Q9M2P44.1e-15481.35E3 ubiquitin-protein ligase SINAT2 OS=Arabidopsis thaliana OX=3702 GN=SINAT2 PE=... [more]
P937487.2e-15179.49Putative E3 ubiquitin-protein ligase SINAT1 OS=Arabidopsis thaliana OX=3702 GN=S... [more]
Q10L912.6e-13279.92E3 ubiquitin-protein ligase DIS1 OS=Oryza sativa subsp. japonica OX=39947 GN=DIS... [more]
Q84JL39.8e-13275.00E3 ubiquitin-protein ligase SINAT3 OS=Arabidopsis thaliana OX=3702 GN=SINAT3 PE=... [more]
Q9STN81.1e-12769.57E3 ubiquitin-protein ligase SINAT4 OS=Arabidopsis thaliana OX=3702 GN=SINAT4 PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1DL038.8e-17693.65RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11102206... [more]
A0A6J1ESE82.8e-17493.89RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111435553... [more]
A0A6J1HT858.2e-17493.57RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111467239 P... [more]
A0A6J1EBG41.0e-17192.93RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111432512... [more]
A0A6J1HS091.1e-17092.93RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111466063 P... [more]
Match NameE-valueIdentityDescription
AT3G58040.12.9e-15581.35seven in absentia of Arabidopsis 2 [more]
AT2G41980.15.1e-15279.49Protein with RING/U-box and TRAF-like domains [more]
AT3G61790.17.0e-13375.00Protein with RING/U-box and TRAF-like domains [more]
AT4G27880.18.0e-12969.57Protein with RING/U-box and TRAF-like domains [more]
AT5G53360.13.2e-10178.92TRAF-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 116..170
e-value: 1.1E-18
score: 69.3
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 25..115
e-value: 5.7E-8
score: 34.4
IPR008974TRAF-likeGENE3D2.60.210.10Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2; Chain Acoord: 171..304
e-value: 8.8E-38
score: 131.0
IPR018121Seven-in-absentia protein, TRAF-like domainPFAMPF03145Sinacoord: 105..304
e-value: 8.9E-81
score: 270.6
NoneNo IPR availablePANTHERPTHR10315E3 UBIQUITIN PROTEIN LIGASE SIAHcoord: 1..310
NoneNo IPR availablePANTHERPTHR10315:SF123E3 UBIQUITIN-PROTEIN LIGASE SINAT2-LIKEcoord: 1..310
NoneNo IPR availableCDDcd03829Sinacoord: 177..305
e-value: 7.3838E-62
score: 190.676
NoneNo IPR availableCDDcd16571RING-HC_SIAHscoord: 61..99
e-value: 9.24729E-13
score: 59.6058
NoneNo IPR availableSUPERFAMILY49599TRAF domain-likecoord: 116..308
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 57..104
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 63..99
score: 9.391148
IPR013010Zinc finger, SIAH-typePROSITEPS51081ZF_SIAHcoord: 116..176
score: 14.552414

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr012141.1Sgr012141.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0007275 multicellular organism development
biological_process GO:0016567 protein ubiquitination
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0016874 ligase activity
molecular_function GO:0005515 protein binding
molecular_function GO:0061630 ubiquitin protein ligase activity
molecular_function GO:0008270 zinc ion binding