Sgr012121 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr012121 vs. NCBI nr
Match: XP_022138215.1 (vinorine synthase-like isoform X2 [Momordica charantia])

HSP 1 Score: 310.8 bits (795), Expect = 1.2e-80
Identity = 171/262 (65.27%), Postives = 199/262 (75.95%), Query Frame = 0

Query: 3   NDDRKTFKVSLVDMLSPEVYIPITFFYSMNENNAHFNKEDVTARLKLSLSETLAHFPMLA 62
           N+D KTFKVS++D+LSP+ Y PITFFYSMN N  H    + T+RLKLSLS+ LA FPM+A
Sbjct: 18  NNDFKTFKVSMLDILSPDSYTPITFFYSMNNNQQH--SHNATSRLKLSLSQALARFPMVA 77

Query: 63  GRIKGDLVHCS-DEDVGALFVEATIKVAMADFLKTPNLQLLAQFLPCPLVLNEPLEIAVQ 122
           G+ KGD +HCS +EDVGALFVEA +K++MA+FLK PNLQLLA FLP   + N PLE AVQ
Sbjct: 78  GKFKGDSIHCSNEEDVGALFVEAEVKMSMAEFLKAPNLQLLANFLPL-FIPNGPLEKAVQ 137

Query: 123 GAAQVNFFTCGGIAIGLSFLHKIIDGTTKSAFIKSWAASCDETLEKLVCGSYSAASSLFP 182
            A QVN F CGGIAIG SFLHKIIDGTT SAF++SWAASC+  + KLVCG+++  SSLFP
Sbjct: 138 IAVQVNVFECGGIAIGASFLHKIIDGTTMSAFLRSWAASCEGKMGKLVCGNFNWGSSLFP 197

Query: 183 LREISLDYRLISPKTPFVVSQGKNSTTRRFVFDAMAISNLKSKAKSARVLNPTSVEVVTC 242
            REI   Y L S   PF V QGK+ST RRFVFDAMAI+NLK +AKS  V NPTSVEVVTC
Sbjct: 198 TREIWDGYNLFS---PFSVKQGKSSTARRFVFDAMAIANLKGRAKSTSVPNPTSVEVVTC 257

Query: 243 -----AVKASR-CRSLGKNVFV 258
                A+KASR C S  K  FV
Sbjct: 258 FIWKYAIKASRSCTSTDKKEFV 273

BLAST of Sgr012121 vs. NCBI nr
Match: XP_022137834.1 (BAHD acyltransferase BIA1-like [Momordica charantia])

HSP 1 Score: 309.3 bits (791), Expect = 3.4e-80
Identity = 174/266 (65.41%), Postives = 203/266 (76.32%), Query Frame = 0

Query: 1   MSNDDRKTFKVSLVDMLSPEVYIPITFFYSMNE--NNAH-FNKEDVTARLKLSLSETLAH 60
           M  DD KTF+VSL+D +SP  YIPI FFYSM E  +N+H F KE+   RLK+SLS+TLAH
Sbjct: 19  MPQDDPKTFQVSLLDKVSPHAYIPIIFFYSMKEETDNSHIFFKENTATRLKISLSKTLAH 78

Query: 61  FPMLAGRIKGDLVHCSDED--VGALFVEATI-KVAMADFLKTPNLQLLAQFLPCPLVLNE 120
           FP+L G+IKG+L+HCS+ED  VGALFVEA + K+ M +FLK PNLQ LAQFLP P   NE
Sbjct: 79  FPILTGKIKGNLIHCSNEDDEVGALFVEAKVQKITMGNFLKNPNLQFLAQFLPFPAAPNE 138

Query: 121 PLEIAVQGAAQVNFFTCGGIAIGLSFLHKIIDGTTKSAFIKSWAASCD--ETLEK-LVCG 180
           PLE  +Q  AQV+FF+CGG+AIG SF HKIIDGTT  AFIKS AA+    E + K LVC 
Sbjct: 139 PLERTIQIGAQVSFFSCGGLAIGFSFFHKIIDGTTMRAFIKSLAAAASNFEGISKNLVCE 198

Query: 181 SYS-AASSLFPLREISLDYRLISPKTPFVVSQGKNSTTRRFVFDAMAISNLKSKAKSARV 240
            Y  + SSLFP REIS DY L S KTPFVV+QGKNST RRFVFD+MAISNLK++AKS RV
Sbjct: 199 GYQYSTSSLFPAREISADYNLFSSKTPFVVAQGKNSTCRRFVFDSMAISNLKARAKSTRV 258

Query: 241 LNPTSVEVVTC-----AVKASRCRSL 252
           LNPT+VEVVTC     A+KASR R L
Sbjct: 259 LNPTTVEVVTCIIWKYAMKASRRRRL 284

BLAST of Sgr012121 vs. NCBI nr
Match: XP_022138214.1 (vinorine synthase-like isoform X1 [Momordica charantia])

HSP 1 Score: 306.6 bits (784), Expect = 2.2e-79
Identity = 166/251 (66.14%), Postives = 194/251 (77.29%), Query Frame = 0

Query: 3   NDDRKTFKVSLVDMLSPEVYIPITFFYSMNENNAHFNKEDVTARLKLSLSETLAHFPMLA 62
           N+D KTFKVS++D+LSP+ Y PITFFYSMN N  H    + T+RLKLSLS+ LA FPM+A
Sbjct: 18  NNDFKTFKVSMLDILSPDSYTPITFFYSMNNNQQH--SHNATSRLKLSLSQALARFPMVA 77

Query: 63  GRIKGDLVHCS-DEDVGALFVEATIKVAMADFLKTPNLQLLAQFLPCPLVLNEPLEIAVQ 122
           G+ KGD +HCS +EDVGALFVEA +K++MA+FLK PNLQLLA FLP   + N PLE AVQ
Sbjct: 78  GKFKGDSIHCSNEEDVGALFVEAEVKMSMAEFLKAPNLQLLANFLPL-FIPNGPLEKAVQ 137

Query: 123 GAAQVNFFTCGGIAIGLSFLHKIIDGTTKSAFIKSWAASCDETLEKLVCGSYSAASSLFP 182
            A QVN F CGGIAIG SFLHKIIDGTT SAF++SWAASC+  + KLVCG+++  SSLFP
Sbjct: 138 IAVQVNVFECGGIAIGASFLHKIIDGTTMSAFLRSWAASCEGKMGKLVCGNFNWGSSLFP 197

Query: 183 LREISLDYRLISPKTPFVVSQGKNSTTRRFVFDAMAISNLKSKAKSARVLNPTSVEVVTC 242
            REI   Y L S   PF V QGK+ST RRFVFDAMAI+NLK +AKS  V NPTSVEVVTC
Sbjct: 198 TREIWDGYNLFS---PFSVKQGKSSTARRFVFDAMAIANLKGRAKSTSVPNPTSVEVVTC 257

Query: 243 -----AVKASR 248
                A+KASR
Sbjct: 258 FIWKYAIKASR 262

BLAST of Sgr012121 vs. NCBI nr
Match: XP_038891571.1 (stemmadenine O-acetyltransferase-like [Benincasa hispida])

HSP 1 Score: 306.2 bits (783), Expect = 2.8e-79
Identity = 165/273 (60.44%), Postives = 193/273 (70.70%), Query Frame = 0

Query: 4   DDRKTFKVSLVDMLSPEVYIPITFFYSMNENNAHFNKEDVTARLKLSLSETLAHFPMLAG 63
           +D+KTFKVS +DMLSPE YIPITFFY MNE N HFNKE+ T RLKLSLS+ LA FP+LA 
Sbjct: 24  NDQKTFKVSFMDMLSPECYIPITFFYPMNETNCHFNKEETTCRLKLSLSKALAQFPILAA 83

Query: 64  RIKGDLVHCSDED-----------VGALFVEATIKVAMADFLKTPNLQLLAQFLPCPLVL 123
           RIKG+L+H  D D           +GALFV+A I++ MA+FLK+PNL LL +FLP P + 
Sbjct: 84  RIKGNLIHSYDGDEDEDEDEDDIIIGALFVDANIQITMAEFLKSPNLNLLPKFLPFPFLS 143

Query: 124 NEPLEIAVQGAAQVNFFTCGGIAIGLSFLHKIIDGTTKSAFIKSWAASCDE---TLEKLV 183
           N+PLE A+Q   QVNFF+CGGI IGLS LH +ID TT S FIK WAA  ++     EKL 
Sbjct: 144 NQPLERAMQIGVQVNFFSCGGIGIGLSLLHTLIDATTMSCFIKCWAAFSEDEGILFEKLG 203

Query: 184 C--GSYSAASSLFPLREISLDYRLISPKTPFVVSQGKNSTTRRFVFDAMAISNLKSKAKS 243
           C  G YS   SLFP REIS  +RLIS +TPFVV +GK STTRRF FD  AISNLK KAKS
Sbjct: 204 CGGGDYSVTCSLFPYREISDGFRLISSETPFVVEKGKKSTTRRFYFDGKAISNLKEKAKS 263

Query: 244 ARVLNPTSVEVVTCAV------KASRCRSLGKN 255
            R  NPT+VEV TC +       ASRC+SL  N
Sbjct: 264 TRAPNPTTVEVTTCFIWKYAMKAASRCKSLDVN 296

BLAST of Sgr012121 vs. NCBI nr
Match: XP_022149427.1 (BAHD acyltransferase At5g47980-like isoform X1 [Momordica charantia])

HSP 1 Score: 286.6 bits (732), Expect = 2.3e-73
Identity = 162/266 (60.90%), Postives = 197/266 (74.06%), Query Frame = 0

Query: 3   NDDRKTFKVSLVDM-LSPEVYIPITFFYSMNENNAHFNKEDVTARLKLSLSETLAHFPML 62
           +  +KTF +SL+D+ +  + Y PI  FYS  +N ++ N E+  + LKLSLS+TLA FPML
Sbjct: 19  DQQQKTFPLSLLDIYIGGQTYTPIILFYSTKKNQSN-NNENTISSLKLSLSKTLARFPML 78

Query: 63  AGRIKGDLVHCSDEDVGALFVEATIKVAMADFLKTPNLQLLAQFLPCP-LVLNEPLEIAV 122
           AGRIKG+ V     + GALFVEA++K +MADFLK P LQ LA  +P P +VL EPLE AV
Sbjct: 79  AGRIKGNFVDYCSYNNGALFVEASVKASMADFLKNPVLQFLANLIPFPDIVLKEPLETAV 138

Query: 123 QGAAQVNFFTCG-GIAIGLSFLHKIIDGTTKSAFIKSWAASCDETLEKLVCGSYSAASSL 182
           Q AAQVNFF+CG GIA+G SFLHK+IDG T SAFIK+WAAS + TLEKL CGSY A+SSL
Sbjct: 139 QVAAQVNFFSCGTGIALGFSFLHKMIDGATMSAFIKTWAASNEGTLEKLACGSYEASSSL 198

Query: 183 FPLREISLDYRLISPKTPFVVSQGKNSTTRRFVFDAMAISNLKSKAKSARVLNPTSVEVV 242
           FP REIS  Y LIS +TPF+V+QGK S++RRFVFDAMA+SNLK +AKS  V NPTSVE V
Sbjct: 199 FPPREISSGYNLISSETPFLVAQGKQSSSRRFVFDAMAVSNLKVRAKSQSVPNPTSVEAV 258

Query: 243 TC-----AVKA-SRCRSLGKNVFVFN 260
           TC     A+KA S C+SL     V N
Sbjct: 259 TCFIWKYAMKATSTCKSLDNKEVVGN 283

BLAST of Sgr012121 vs. ExPASy Swiss-Prot
Match: O23393 (BAHD acyltransferase BIA1 OS=Arabidopsis thaliana OX=3702 GN=BIA1 PE=2 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 2.2e-21
Identity = 84/261 (32.18%), Postives = 127/261 (48.66%), Query Frame = 0

Query: 2   SNDDRKTFKVSLVDMLSPEVYIPITFFYSMNENNAHFNKEDVTARLKLSLSETLAHFPML 61
           S   R   ++S++D+  P +Y+   FFY +   ++    E+    LKLSLSETL+ F  L
Sbjct: 17  SPSPRDRLQLSILDLYCPGIYVSTIFFYDLITESSEVFSEN----LKLSLSETLSRFYPL 76

Query: 62  AGRIKGDLVHCSDEDVGALFVEATIKVAMADFLKTPNLQLLAQFLPCPLVLNEPLEIAVQ 121
           AGRI+G  + C+DE  GA+F EA   + + DFL+  N   L+ FLP       P    + 
Sbjct: 77  AGRIEGLSISCNDE--GAVFTEARTDLLLPDFLRNLNTDSLSGFLPTLAAGESPAAWPLL 136

Query: 122 GAAQVNFFTCG-GIAIGLSFLHKIIDGTTKSAFIKSWAAS-----CDETLEKLVCGSYSA 181
            + +V FF  G G+A+ +S  HKI D  +   F+K WA +      + T+E        A
Sbjct: 137 -SVKVTFFGSGSGVAVSVSVSHKICDIASLVTFVKDWATTTAKGKSNSTIE-------FA 196

Query: 182 ASSLFPLREISLDYRLISPKTPFVVSQGKNSTTRRFVFDAMAISNLKSKAKSARVLNPTS 241
            ++++P     +  +   P T    +       +RFVF+   I+ LK KA S  V  PT 
Sbjct: 197 ETTIYPPPPSHMYEQF--PSTDSDSNITSKYVLKRFVFEPSKIAELKHKAASESVPVPTR 256

Query: 242 VEVV-----TCAVKASRCRSL 252
           VE +      CA  +SR   L
Sbjct: 257 VEAIMSLIWRCARNSSRSNLL 261

BLAST of Sgr012121 vs. ExPASy Swiss-Prot
Match: Q94FT4 (Salutaridinol 7-O-acetyltransferase OS=Papaver somniferum OX=3469 GN=SALAT PE=1 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 1.1e-20
Identity = 97/272 (35.66%), Postives = 133/272 (48.90%), Query Frame = 0

Query: 7   KTFKVSLVDMLSP-EVYIPITFFYSMNENNA--HFNKEDVTARLKLSLSETLAHFPMLAG 66
           K F +SL+D   P   Y+PI  FY     N+    N  D    LK SLS+TL HF  +AG
Sbjct: 28  KNFNLSLLDQCFPLYYYVPIILFYPATAANSTGSSNHHDDLDLLKSSLSKTLVHFYPMAG 87

Query: 67  R-IKGDLVHCSDEDVGALFVEATIKVAMADFLKTPNLQLLAQFLPCPLV-LNEPLEIAVQ 126
           R I   LV C D+  G  F +  I+  M +F+  P++  L+Q LP  +V  + P E  V 
Sbjct: 88  RMIDNILVDCHDQ--GINFYKVKIRGKMCEFMSQPDVP-LSQLLPSEVVSASVPKEALV- 147

Query: 127 GAAQVNFFTCGGIAIGLSFLHKIIDGTTKSAFIKSWAASCDE----------TLEKLVCG 186
              QVN F CGG AI  S  HKI D  T S FI+SWA++             T +KL+  
Sbjct: 148 -IVQVNMFDCGGTAICSSVSHKIADAATMSTFIRSWASTTKTSRSGGSTAAVTDQKLIPS 207

Query: 187 SYSAASSLFPLREISLDYRLISP----KTPFVV----SQGKNSTTRRFVFDAMAISNLKS 246
             SA  SLFP  E     RL SP    + PF      ++   + ++RFVFD   I++++ 
Sbjct: 208 FDSA--SLFPPSE-----RLTSPSGMSEIPFSSTPEDTEDDKTVSKRFVFDFAKITSVRE 267

Query: 247 KAK-----SARVLNPTSVEVVTCAVKASRCRS 251
           K +     + +    T VEVVT  +  S  +S
Sbjct: 268 KLQVLMHDNYKSRRQTRVEVVTSLIWKSVMKS 287

BLAST of Sgr012121 vs. ExPASy Swiss-Prot
Match: Q9ZTK5 (Deacetylvindoline O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=DAT PE=1 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 9.1e-20
Identity = 82/256 (32.03%), Postives = 125/256 (48.83%), Query Frame = 0

Query: 9   FKVSLVDMLSPEVYIPITFFYSMNENNAHFNKEDVTARLKLSLSETLAHFPMLAGR-IKG 68
           + +S  D    +  + + FFY   EN        +  +L+ SLS+TL  +   AG+ +K 
Sbjct: 31  YNLSYNDQNIYQTCVSVGFFY---ENPDGIEISTIREQLQNSLSKTLVSYYPFAGKVVKN 90

Query: 69  DLVHCSDEDVGALFVEATIKVAMADFLKTPNLQLLAQFLPCPLVLNEPLEIAVQGAAQVN 128
           D +HC+D+  G  FVE  I+  M D LK   L+  A+ L  P  +    E       Q++
Sbjct: 91  DYIHCNDD--GIEFVEVRIRCRMNDILKY-ELRSYARDLVLPKRVTVGSE-DTTAIVQLS 150

Query: 129 FFTCGGIAIGLSFLHKIIDGTTKSAFIKSWAAS-CDETLEKLVCGSYSAASSLFPLREIS 188
            F CGG+A+     HK+ DG T ++F+K WAAS C  +    V      + S+FP ++  
Sbjct: 151 HFDCGGLAVAFGISHKVADGGTIASFMKDWAASACYLSSSHHVPTPLLVSDSIFPRQD-- 210

Query: 189 LDYRLISPKTPFVVSQGKNSTTRRFVFDAMAISNLKSKAKSARVLNPTSVEVVTCAVKAS 248
               +I  + P      KN   + F+F   AI  LKSKA    +  PT VEV+T  +  S
Sbjct: 211 ---NIICEQFP----TSKNCVEKTFIFPPEAIEKLKSKAVEFGIEKPTRVEVLTAFL--S 268

Query: 249 RCRSL-GKNVFVFNDC 262
           RC ++ GK+    N+C
Sbjct: 271 RCATVAGKSAAKNNNC 268

BLAST of Sgr012121 vs. ExPASy Swiss-Prot
Match: A0A2K8FQU5 (Tabersonine-19-hydroxy-O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=TAT PE=1 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 7.7e-19
Identity = 81/237 (34.18%), Postives = 114/237 (48.10%), Query Frame = 0

Query: 7   KTFKVSLVDMLSPEVYIPITFFYSMNENNAHFNKEDVTARLKLSLSETLAHFPMLAGR-I 66
           + + +S +D         + FFY+ N ++      D+   LK SLS+TL  +   AGR I
Sbjct: 25  RRYNLSSIDQTIDSEVTSLAFFYTYNPSHESSKIGDL---LKNSLSKTLVSYYQFAGRLI 84

Query: 67  KGDLVHCSDEDVGALFVEATIKVAMADFLKTPNLQLLAQFLPCPLV-LNEPLEIAVQGAA 126
           + D + C+DE  G  FVE  I   M D LK          LP  ++ L+E   + V    
Sbjct: 85  ENDYIDCNDE--GVEFVEVRIHGRMNDILKRGKSFATDLVLPTRIIALHEDSLLIV---- 144

Query: 127 QVNFFTCGGIAIGLSFLHKIIDGTTKSAFIKSWAASCD-ETLEKLVCGSYSAASSLFPLR 186
           Q++ F CGGIAIG    HK+ DG +   F+K WA+S    T  K        A S+FP  
Sbjct: 145 QLSHFDCGGIAIGFGASHKVSDGVSNVMFMKDWASSTSLSTFHKPT--PLLTADSIFPPE 204

Query: 187 EISLDYRLISPKTPFVVSQGKNSTTRRFVFDAMAISNLKSKAKSARVLNPTSVEVVT 241
               D +L+S K+   +   +    +RFVF   AI  LKSKA    +  P+ VEVVT
Sbjct: 205 ----DNKLLSNKS---IVSFQQCLGKRFVFSTEAIEKLKSKAIEYGIQKPSRVEVVT 243

BLAST of Sgr012121 vs. ExPASy Swiss-Prot
Match: Q8GZU0 (Minovincinine 19-hydroxy-O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=MAT PE=1 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 1.0e-18
Identity = 82/243 (33.74%), Postives = 117/243 (48.15%), Query Frame = 0

Query: 22  YIPITFFYSMNENNAHFNKEDVTARLKLSLSETLAHFPMLAGR-IKGDLVHCSDEDVGAL 81
           YI   FFYS   N        +  +L+ SLS+TL  +   AG+ +K D +HC+D+  G  
Sbjct: 43  YIFAGFFYS---NPDGHEISTIREQLQNSLSKTLVSYYPFAGKVVKNDYIHCNDD--GIE 102

Query: 82  FVEATIKVAMADFLKTPNLQLLAQFLPCPLVLNEPLEIAVQGAAQVNFFTCGGIAIGLSF 141
           FV+  I   M D LK P L+  A  L  P       E +     Q++ F CGG+A+    
Sbjct: 103 FVDVRIHCRMNDILK-PELRSYASELIRPNRSTVGSEDST-ALVQLSHFDCGGVAVAFGI 162

Query: 142 LHKIIDGTTKSAFIKSWAAS-CDETLEKLVCGSYSAASSLFPLREISLDYRLISPKTPFV 201
            HK+ D  T  +FIK WAAS CD +    V      + S+FP ++      +I  + P  
Sbjct: 163 SHKVADAATILSFIKDWAASTCDLSSSHDVSTPVLVSDSIFPRQD-----NIICGQFP-- 222

Query: 202 VSQGKNSTTRRFVFDAMAISNLKSKAKSARVLNPTSVEVVTCAVKASRCRSL-GKNVFVF 261
                N   +RF+F   AI  LKSKA    +  PT VEV+T  +   RC ++ GK+    
Sbjct: 223 --ASPNCVRKRFLFSPEAIERLKSKAIEFGIEKPTRVEVLTAFL--CRCATVAGKSAAKN 267

BLAST of Sgr012121 vs. ExPASy TrEMBL
Match: A0A6J1C8U3 (vinorine synthase-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111009443 PE=3 SV=1)

HSP 1 Score: 310.8 bits (795), Expect = 5.6e-81
Identity = 171/262 (65.27%), Postives = 199/262 (75.95%), Query Frame = 0

Query: 3   NDDRKTFKVSLVDMLSPEVYIPITFFYSMNENNAHFNKEDVTARLKLSLSETLAHFPMLA 62
           N+D KTFKVS++D+LSP+ Y PITFFYSMN N  H    + T+RLKLSLS+ LA FPM+A
Sbjct: 18  NNDFKTFKVSMLDILSPDSYTPITFFYSMNNNQQH--SHNATSRLKLSLSQALARFPMVA 77

Query: 63  GRIKGDLVHCS-DEDVGALFVEATIKVAMADFLKTPNLQLLAQFLPCPLVLNEPLEIAVQ 122
           G+ KGD +HCS +EDVGALFVEA +K++MA+FLK PNLQLLA FLP   + N PLE AVQ
Sbjct: 78  GKFKGDSIHCSNEEDVGALFVEAEVKMSMAEFLKAPNLQLLANFLPL-FIPNGPLEKAVQ 137

Query: 123 GAAQVNFFTCGGIAIGLSFLHKIIDGTTKSAFIKSWAASCDETLEKLVCGSYSAASSLFP 182
            A QVN F CGGIAIG SFLHKIIDGTT SAF++SWAASC+  + KLVCG+++  SSLFP
Sbjct: 138 IAVQVNVFECGGIAIGASFLHKIIDGTTMSAFLRSWAASCEGKMGKLVCGNFNWGSSLFP 197

Query: 183 LREISLDYRLISPKTPFVVSQGKNSTTRRFVFDAMAISNLKSKAKSARVLNPTSVEVVTC 242
            REI   Y L S   PF V QGK+ST RRFVFDAMAI+NLK +AKS  V NPTSVEVVTC
Sbjct: 198 TREIWDGYNLFS---PFSVKQGKSSTARRFVFDAMAIANLKGRAKSTSVPNPTSVEVVTC 257

Query: 243 -----AVKASR-CRSLGKNVFV 258
                A+KASR C S  K  FV
Sbjct: 258 FIWKYAIKASRSCTSTDKKEFV 273

BLAST of Sgr012121 vs. ExPASy TrEMBL
Match: A0A6J1C8E0 (BAHD acyltransferase BIA1-like OS=Momordica charantia OX=3673 GN=LOC111009175 PE=3 SV=1)

HSP 1 Score: 309.3 bits (791), Expect = 1.6e-80
Identity = 174/266 (65.41%), Postives = 203/266 (76.32%), Query Frame = 0

Query: 1   MSNDDRKTFKVSLVDMLSPEVYIPITFFYSMNE--NNAH-FNKEDVTARLKLSLSETLAH 60
           M  DD KTF+VSL+D +SP  YIPI FFYSM E  +N+H F KE+   RLK+SLS+TLAH
Sbjct: 19  MPQDDPKTFQVSLLDKVSPHAYIPIIFFYSMKEETDNSHIFFKENTATRLKISLSKTLAH 78

Query: 61  FPMLAGRIKGDLVHCSDED--VGALFVEATI-KVAMADFLKTPNLQLLAQFLPCPLVLNE 120
           FP+L G+IKG+L+HCS+ED  VGALFVEA + K+ M +FLK PNLQ LAQFLP P   NE
Sbjct: 79  FPILTGKIKGNLIHCSNEDDEVGALFVEAKVQKITMGNFLKNPNLQFLAQFLPFPAAPNE 138

Query: 121 PLEIAVQGAAQVNFFTCGGIAIGLSFLHKIIDGTTKSAFIKSWAASCD--ETLEK-LVCG 180
           PLE  +Q  AQV+FF+CGG+AIG SF HKIIDGTT  AFIKS AA+    E + K LVC 
Sbjct: 139 PLERTIQIGAQVSFFSCGGLAIGFSFFHKIIDGTTMRAFIKSLAAAASNFEGISKNLVCE 198

Query: 181 SYS-AASSLFPLREISLDYRLISPKTPFVVSQGKNSTTRRFVFDAMAISNLKSKAKSARV 240
            Y  + SSLFP REIS DY L S KTPFVV+QGKNST RRFVFD+MAISNLK++AKS RV
Sbjct: 199 GYQYSTSSLFPAREISADYNLFSSKTPFVVAQGKNSTCRRFVFDSMAISNLKARAKSTRV 258

Query: 241 LNPTSVEVVTC-----AVKASRCRSL 252
           LNPT+VEVVTC     A+KASR R L
Sbjct: 259 LNPTTVEVVTCIIWKYAMKASRRRRL 284

BLAST of Sgr012121 vs. ExPASy TrEMBL
Match: A0A6J1C9H0 (vinorine synthase-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111009443 PE=3 SV=1)

HSP 1 Score: 306.6 bits (784), Expect = 1.1e-79
Identity = 166/251 (66.14%), Postives = 194/251 (77.29%), Query Frame = 0

Query: 3   NDDRKTFKVSLVDMLSPEVYIPITFFYSMNENNAHFNKEDVTARLKLSLSETLAHFPMLA 62
           N+D KTFKVS++D+LSP+ Y PITFFYSMN N  H    + T+RLKLSLS+ LA FPM+A
Sbjct: 18  NNDFKTFKVSMLDILSPDSYTPITFFYSMNNNQQH--SHNATSRLKLSLSQALARFPMVA 77

Query: 63  GRIKGDLVHCS-DEDVGALFVEATIKVAMADFLKTPNLQLLAQFLPCPLVLNEPLEIAVQ 122
           G+ KGD +HCS +EDVGALFVEA +K++MA+FLK PNLQLLA FLP   + N PLE AVQ
Sbjct: 78  GKFKGDSIHCSNEEDVGALFVEAEVKMSMAEFLKAPNLQLLANFLPL-FIPNGPLEKAVQ 137

Query: 123 GAAQVNFFTCGGIAIGLSFLHKIIDGTTKSAFIKSWAASCDETLEKLVCGSYSAASSLFP 182
            A QVN F CGGIAIG SFLHKIIDGTT SAF++SWAASC+  + KLVCG+++  SSLFP
Sbjct: 138 IAVQVNVFECGGIAIGASFLHKIIDGTTMSAFLRSWAASCEGKMGKLVCGNFNWGSSLFP 197

Query: 183 LREISLDYRLISPKTPFVVSQGKNSTTRRFVFDAMAISNLKSKAKSARVLNPTSVEVVTC 242
            REI   Y L S   PF V QGK+ST RRFVFDAMAI+NLK +AKS  V NPTSVEVVTC
Sbjct: 198 TREIWDGYNLFS---PFSVKQGKSSTARRFVFDAMAIANLKGRAKSTSVPNPTSVEVVTC 257

Query: 243 -----AVKASR 248
                A+KASR
Sbjct: 258 FIWKYAIKASR 262

BLAST of Sgr012121 vs. ExPASy TrEMBL
Match: A0A6J1D7X5 (BAHD acyltransferase At5g47980-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111017856 PE=3 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 1.1e-73
Identity = 162/266 (60.90%), Postives = 197/266 (74.06%), Query Frame = 0

Query: 3   NDDRKTFKVSLVDM-LSPEVYIPITFFYSMNENNAHFNKEDVTARLKLSLSETLAHFPML 62
           +  +KTF +SL+D+ +  + Y PI  FYS  +N ++ N E+  + LKLSLS+TLA FPML
Sbjct: 19  DQQQKTFPLSLLDIYIGGQTYTPIILFYSTKKNQSN-NNENTISSLKLSLSKTLARFPML 78

Query: 63  AGRIKGDLVHCSDEDVGALFVEATIKVAMADFLKTPNLQLLAQFLPCP-LVLNEPLEIAV 122
           AGRIKG+ V     + GALFVEA++K +MADFLK P LQ LA  +P P +VL EPLE AV
Sbjct: 79  AGRIKGNFVDYCSYNNGALFVEASVKASMADFLKNPVLQFLANLIPFPDIVLKEPLETAV 138

Query: 123 QGAAQVNFFTCG-GIAIGLSFLHKIIDGTTKSAFIKSWAASCDETLEKLVCGSYSAASSL 182
           Q AAQVNFF+CG GIA+G SFLHK+IDG T SAFIK+WAAS + TLEKL CGSY A+SSL
Sbjct: 139 QVAAQVNFFSCGTGIALGFSFLHKMIDGATMSAFIKTWAASNEGTLEKLACGSYEASSSL 198

Query: 183 FPLREISLDYRLISPKTPFVVSQGKNSTTRRFVFDAMAISNLKSKAKSARVLNPTSVEVV 242
           FP REIS  Y LIS +TPF+V+QGK S++RRFVFDAMA+SNLK +AKS  V NPTSVE V
Sbjct: 199 FPPREISSGYNLISSETPFLVAQGKQSSSRRFVFDAMAVSNLKVRAKSQSVPNPTSVEAV 258

Query: 243 TC-----AVKA-SRCRSLGKNVFVFN 260
           TC     A+KA S C+SL     V N
Sbjct: 259 TCFIWKYAMKATSTCKSLDNKEVVGN 283

BLAST of Sgr012121 vs. ExPASy TrEMBL
Match: A0A6J1D5L8 (BAHD acyltransferase At5g47980-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111017835 PE=3 SV=1)

HSP 1 Score: 280.8 bits (717), Expect = 6.2e-72
Identity = 156/261 (59.77%), Postives = 194/261 (74.33%), Query Frame = 0

Query: 6   RKTFKVSLVDMLSPEVYIPITFFYSMNENNAHFNKEDVTARLKLSLSETLAHFPMLAGRI 65
           +KTF++SL+D+ + E Y P   FY+  + +   N E+  + LKLSLS+TLA FPMLAGRI
Sbjct: 26  QKTFQLSLLDIYASETYSPFILFYN-TKKDQRSNNENAISSLKLSLSKTLARFPMLAGRI 85

Query: 66  KGDLVHCSDEDVGALFVEATIKVAMADFLKTPNLQLLAQFLPCP-LVLNEPLEIAVQGAA 125
           KG+ VH    + GALFVEA++K +MADFL+TPNLQ LA  +P P LVL +PLE AVQ AA
Sbjct: 86  KGNFVHYCSSNNGALFVEASVKTSMADFLRTPNLQFLADLIPFPDLVLKQPLETAVQVAA 145

Query: 126 QVNFFTCG-GIAIGLSFLHKIIDGTTKSAFIKSWAASCDETLEKLVCGSYSAASSLFPLR 185
           QVNFF+CG GIA+G SFLHK+IDG T SAFIK+WAAS + TL+KL CGSY A+SSLFP R
Sbjct: 146 QVNFFSCGTGIALGFSFLHKMIDGATMSAFIKTWAASNEGTLDKLACGSYKASSSLFPAR 205

Query: 186 EISLDYR-LISPKTPFVVSQGKNSTTRRFVFDAMAISNLKSKAKSARVLNPTSVEVVTC- 245
           EIS +Y  L+ P+T  +V+Q K+ + RRFVFDAMA+SNLK +AKS  V NPTSVE VTC 
Sbjct: 206 EISSNYNDLVFPETALLVAQPKHCSVRRFVFDAMAVSNLKVRAKSQSVSNPTSVEAVTCF 265

Query: 246 ----AVKA-SRCRSLGKNVFV 258
               A+KA S C+SL K   V
Sbjct: 266 IWKYAMKATSTCKSLDKKEVV 285

BLAST of Sgr012121 vs. TAIR 10
Match: AT1G24420.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 134.4 bits (337), Expect = 1.4e-31
Identity = 96/254 (37.80%), Postives = 135/254 (53.15%), Query Frame = 0

Query: 4   DDRKTFKVSLVDMLSPEVYIPITFFYSMN-ENNAHFNKEDVTARLKLSLSETLAHFPMLA 63
           DD++   +SL+D+LS  +Y     FY+ + +N   F+ E+ + +LK SLS+TL  F  LA
Sbjct: 21  DDKRILNLSLLDILSSPMYTGALLFYAADPQNLLGFSTEETSLKLKKSLSKTLPIFYPLA 80

Query: 64  GRIKGDLVHCSDEDVGALFVEATIKVAMADFLKTPNLQLLAQFLPCPLVLNEPLEIAVQG 123
           GRI G  V C+DE  GA+F+EA +   +++FLK P  + L   +P      E +   V  
Sbjct: 81  GRIIGSFVECNDE--GAVFIEARVDHLLSEFLKCPVPESLELLIPVEAKSREAVTWPVL- 140

Query: 124 AAQVNFFTCGGIAIGLSFLHKIIDGTTKSAFIKSWAASCDETLEKLVCGSYSAASSLFPL 183
             Q NFF+CGG+ I +   HKI D T+ + FI+ WA S    L   +  S++ AS +FP 
Sbjct: 141 LIQANFFSCGGLVITICVSHKITDATSLAMFIRGWAES-SRGLGITLIPSFT-ASEVFPK 200

Query: 184 REISLDYRLISPKTPFVVSQGKNSTTRRFVFDAMAISNLKSKAKSARVLNPTSVEVVT-- 243
               LD     P       +  +  T+RFVFDA  I  L++KA    V NPT VE VT  
Sbjct: 201 ---PLDELPSKPMDRKEEVEEMSCVTKRFVFDASKIKKLRAKASRNLVKNPTRVEAVTAL 260

Query: 244 ---CAVKASRCRSL 252
              C  K SR  SL
Sbjct: 261 FWRCVTKVSRLSSL 266

BLAST of Sgr012121 vs. TAIR 10
Match: AT3G30280.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 122.9 bits (307), Expect = 4.2e-28
Identity = 88/245 (35.92%), Postives = 130/245 (53.06%), Query Frame = 0

Query: 4   DDRKTFKVSLVDMLSPEVYIPITFFYSMNENNAHFNKEDVTARLKLSLSETLAHFPMLAG 63
           +D +T ++S+ D + P VY     FY+ ++     + E  + +LK SL+ETL  F  LAG
Sbjct: 22  NDLQTLQLSIYDHILPPVYTVAFLFYTKDD---LISPEQSSHKLKTSLAETLTKFYPLAG 81

Query: 64  RIKGDLVHCSDEDVGALFVEATI-KVAMADFLKTPNLQLLAQFLPCPLVLNEPLEIAVQG 123
           RIKG  + C+DE  GA+FV+A +    ++DFL++P+ + L Q LP   V+++P E A   
Sbjct: 82  RIKGVTIDCNDE--GAVFVDARVNNYPLSDFLRSPDFKTLQQLLPLD-VIDDPYEAATTW 141

Query: 124 ---AAQVNFFTCGGIAIGLSFLHKIIDGTTKSAFIKSWAASCDETLEKLVCGSYSAASSL 183
                +  +F CGG+AIGL   HKI D T+ S FI+SWAA        +V     AA++ 
Sbjct: 142 PLLLVKATYFPCGGMAIGLCITHKIADATSISTFIQSWAAMARGEAGDIVADPVFAAANF 201

Query: 184 FPLREISLDYRLISPKTPFVVSQGKN---STTRRFVFDAMAISNLKSKAKSAR-VLNPTS 241
           +P           +    F V +  N     T+RFVF A  +  L++KA S   V  P  
Sbjct: 202 YP---------PANELFKFPVDENANKISQITKRFVFSASKLEELRTKAASEDFVGRPKR 251

BLAST of Sgr012121 vs. TAIR 10
Match: AT4G15390.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 122.9 bits (307), Expect = 4.2e-28
Identity = 92/263 (34.98%), Postives = 137/263 (52.09%), Query Frame = 0

Query: 7   KTFKVSLVDMLSPEVYIPITFFYSMNENNAHFNKEDVTARLKLSLSETLAHFPMLAGRIK 66
           +T ++S+ D + P VY     FY+ N+     ++E  + +LK SLSETL  F  LAGRI 
Sbjct: 27  QTLQLSIYDHILPPVYTVAFLFYTKND---LISQEHTSHKLKTSLSETLTKFYPLAGRIT 86

Query: 67  GDLVHCSDEDVGALFVEATI-KVAMADFLKTPNLQLLAQFLPCPLVLNEPLEIAVQG--A 126
           G  V C+DE  GA+FV+A +    + +FLK P+   L Q LP  +V N  +  A      
Sbjct: 87  GVTVDCTDE--GAIFVDARVNNCPLTEFLKCPDFDALQQLLPLDVVDNPYVAAATWPLLL 146

Query: 127 AQVNFFTCGGIAIGLSFLHKIIDGTTKSAFIKSWAASC--DETLEKLVCGSYSAASSLFP 186
            +  +F CGG+AIG+   HKI D  + S FI+SWAA+   +     +    ++ A+   P
Sbjct: 147 VKATYFGCGGMAIGICITHKIADAASISTFIRSWAATARGENDAAAMESPVFAGANFYPP 206

Query: 187 LREISLDYRLISPKTPFVVSQGKNST-TRRFVFDAMAISNLKSKAKSARVLN-PTSVEVV 246
             E        + K P     GK S+ T+RFVF+A  + +L++KA S   ++ PT VE V
Sbjct: 207 ANE--------AFKLPADEQAGKRSSITKRFVFEASKVEDLRTKAASEETVDQPTRVESV 266

Query: 247 T-----CAVKASRCRSLGKNVFV 258
           T     C V +S+  +    V V
Sbjct: 267 TALIWKCFVASSKTTTCDHKVLV 276

BLAST of Sgr012121 vs. TAIR 10
Match: AT3G26040.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 121.3 bits (303), Expect = 1.2e-27
Identity = 84/248 (33.87%), Postives = 132/248 (53.23%), Query Frame = 0

Query: 7   KTFKVSLVDMLSPEVYIPITFFYSMNENNAHFNKEDVTARLKLSLSETLAHFPMLAGRIK 66
           K FK+SL++ L P ++ P+ FFYS N +       +    LK SLSETL HF  LAGR+K
Sbjct: 22  KKFKLSLLEQLGPTIFGPMVFFYSANNS---IKPTEQLQMLKKSLSETLTHFYPLAGRLK 81

Query: 67  GDL-VHCSDEDVGALFVEATIKVAMADFLKTPNLQLLAQFLPCPLVLNEPLEIAVQ-GAA 126
           G++ + C+D   GA F+EA +   +++ L  P+   L Q +P  +   + +E   +   A
Sbjct: 82  GNISIDCNDS--GADFLEARVNSPLSNLLLEPSSDSLQQLIPTSV---DSIETRTRLLLA 141

Query: 127 QVNFFTCGGIAIGLSFLHKIIDGTTKSAFIKSWAASCDETLEKLVCGSYSAASSLFPLRE 186
           Q +FF CG ++IG+   HK+ D T+   F+KSWAA       K +         +FP   
Sbjct: 142 QASFFECGSMSIGVCISHKLADATSIGLFMKSWAAISSRGSIKTIGAPVFDTVKIFPPGN 201

Query: 187 ISLDYRLISPKTPFVVSQGKNSTTRRFVFDAMAISNLKSKAKSARVLNPTSVEVVTC--- 246
            S        +   +++Q   + ++RF+FD+ +I  L++KA S  V  PT VE V+    
Sbjct: 202 FSETSPAPVVEPEIMMNQ---TLSKRFIFDSSSIQALQAKASSFEVNQPTRVEAVSALIW 258

Query: 247 --AVKASR 248
             A+KA+R
Sbjct: 262 KSAMKATR 258

BLAST of Sgr012121 vs. TAIR 10
Match: AT5G47950.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 112.8 bits (281), Expect = 4.3e-25
Identity = 85/248 (34.27%), Postives = 129/248 (52.02%), Query Frame = 0

Query: 5   DRKTFKVSLVDMLSPEVYIPITFFYSMNENNAHFNKEDVTARLKLSLSETLAHFPMLAGR 64
           D  T ++S++D+L P VY     FY+ ++     ++E  +  LK SLSE L  F  LAGR
Sbjct: 23  DLSTLQLSIMDILMPPVYAVAFLFYTKDD---LISQEQTSHTLKTSLSEILTKFHPLAGR 82

Query: 65  IKGDLVHCSDEDVGALFVEATI-KVAMADFLKTPNLQLLAQFLPCPLVLNEPLEIAVQGA 124
           + G  +  +DE  GA+FVEA +    ++ FL++P+ + L Q LP   V +EP        
Sbjct: 83  VNGVTIKSTDE--GAVFVEARVDNCDLSGFLRSPDTESLKQLLP---VDDEPAPTWPLLL 142

Query: 125 AQVNFFTCGGIAIGLSFLHKIIDGTTKSAFIKSWAASCDETLEKLVCGSYSAASSLFPLR 184
            +  +F CGG+AIGL   H++ D  + S F+++WAA+     + LV      ++ L+P  
Sbjct: 143 VKATYFRCGGMAIGLCISHRLADAASLSIFLQAWAATARGESD-LVASPDFCSTKLYPAA 202

Query: 185 EISLDYRLISPKTPFVVSQGKNSTTRRFVFDAMAISNLKSKAKSARVLNPTSVEVVT--- 244
             ++       K P  V   + S T+RFVF A  I  L++K  S  V  PT V+ VT   
Sbjct: 203 NEAI-------KIPGEV-VNRTSVTKRFVFVASKIEELRNKVASDVVPRPTRVQSVTSLI 253

Query: 245 --CAVKAS 247
             C V AS
Sbjct: 263 WKCVVTAS 253