Sgr012117 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr012117 vs. NCBI nr
Match: XP_022138215.1 (vinorine synthase-like isoform X2 [Momordica charantia])

HSP 1 Score: 317.8 bits (813), Expect = 9.9e-83
Identity = 170/280 (60.71%), Postives = 209/280 (74.64%), Query Frame = 0

Query: 1   MEVQIISREVIKPAIP-SDHLKTCQVSLLDMLSPQFYTPITFFYPKKEIGGHLNKEDATA 60
           ME++IISRE+IKP +P ++  KT +VS+LD+LSP  YTPITFFY       H    +AT+
Sbjct: 1   MELEIISREIIKPPMPLNNDFKTFKVSMLDILSPDSYTPITFFYSMNNNQQH--SHNATS 60

Query: 61  RLKLSLSETLVRFSMLAGRIKGDLIHCS-DEGVGALFVEATVTVTMADFLKTPDLQLLAQ 120
           RLKLSLS+ L RF M+AG+ KGD IHCS +E VGALFVEA V ++MA+FLK P+LQLLA 
Sbjct: 61  RLKLSLSQALARFPMVAGKFKGDSIHCSNEEDVGALFVEAEVKMSMAEFLKAPNLQLLAN 120

Query: 121 FLPVPLVPNEPLETFVQVAAQVNFFSCGRIAIGFSFFHKIIDGTTMSAFIKSWAATNEGT 180
           FLP+  +PN PLE  VQ+A QVN F CG IAIG SF HKIIDGTTMSAF++SWAA+ EG 
Sbjct: 121 FLPL-FIPNGPLEKAVQIAVQVNVFECGGIAIGASFLHKIIDGTTMSAFLRSWAASCEGK 180

Query: 181 LEKLECGNYSSASSLFPPREISTSYRLISQETPFAVPQGKSSTTRRFLFDAMAISNLKAK 240
           + KL CGN++  SSLFP REI   Y L S   PF+V QGKSST RRF+FDAMAI+NLK +
Sbjct: 181 MGKLVCGNFNWGSSLFPTREIWDGYNLFS---PFSVKQGKSSTARRFVFDAMAIANLKGR 240

Query: 241 TRSAHVSNPTSVEAVTCFIWKYVMEASRYRSFGESKSILN 279
            +S  V NPTSVE VTCFIWKY ++ASR  +  + K  ++
Sbjct: 241 AKSTSVPNPTSVEVVTCFIWKYAIKASRSCTSTDKKEFVD 274

BLAST of Sgr012117 vs. NCBI nr
Match: XP_022138214.1 (vinorine synthase-like isoform X1 [Momordica charantia])

HSP 1 Score: 317.0 bits (811), Expect = 1.7e-82
Identity = 169/268 (63.06%), Postives = 204/268 (76.12%), Query Frame = 0

Query: 1   MEVQIISREVIKPAIP-SDHLKTCQVSLLDMLSPQFYTPITFFYPKKEIGGHLNKEDATA 60
           ME++IISRE+IKP +P ++  KT +VS+LD+LSP  YTPITFFY       H    +AT+
Sbjct: 1   MELEIISREIIKPPMPLNNDFKTFKVSMLDILSPDSYTPITFFYSMNNNQQH--SHNATS 60

Query: 61  RLKLSLSETLVRFSMLAGRIKGDLIHCS-DEGVGALFVEATVTVTMADFLKTPDLQLLAQ 120
           RLKLSLS+ L RF M+AG+ KGD IHCS +E VGALFVEA V ++MA+FLK P+LQLLA 
Sbjct: 61  RLKLSLSQALARFPMVAGKFKGDSIHCSNEEDVGALFVEAEVKMSMAEFLKAPNLQLLAN 120

Query: 121 FLPVPLVPNEPLETFVQVAAQVNFFSCGRIAIGFSFFHKIIDGTTMSAFIKSWAATNEGT 180
           FLP+  +PN PLE  VQ+A QVN F CG IAIG SF HKIIDGTTMSAF++SWAA+ EG 
Sbjct: 121 FLPL-FIPNGPLEKAVQIAVQVNVFECGGIAIGASFLHKIIDGTTMSAFLRSWAASCEGK 180

Query: 181 LEKLECGNYSSASSLFPPREISTSYRLISQETPFAVPQGKSSTTRRFLFDAMAISNLKAK 240
           + KL CGN++  SSLFP REI   Y L S   PF+V QGKSST RRF+FDAMAI+NLK +
Sbjct: 181 MGKLVCGNFNWGSSLFPTREIWDGYNLFS---PFSVKQGKSSTARRFVFDAMAIANLKGR 240

Query: 241 TRSAHVSNPTSVEAVTCFIWKYVMEASR 267
            +S  V NPTSVE VTCFIWKY ++ASR
Sbjct: 241 AKSTSVPNPTSVEVVTCFIWKYAIKASR 262

BLAST of Sgr012117 vs. NCBI nr
Match: XP_022137834.1 (BAHD acyltransferase BIA1-like [Momordica charantia])

HSP 1 Score: 316.2 bits (809), Expect = 2.9e-82
Identity = 175/286 (61.19%), Postives = 205/286 (71.68%), Query Frame = 0

Query: 1   MEVQIISREVIKPA----IPSDHLKTCQVSLLDMLSPQFYTPITFFYPKKEIGGHLN--- 60
           MEV+IISRE+IKPA    +P D  KT QVSLLD +SP  Y PI FFY  KE   + +   
Sbjct: 1   MEVEIISREIIKPATLEEMPQDDPKTFQVSLLDKVSPHAYIPIIFFYSMKEETDNSHIFF 60

Query: 61  KEDATARLKLSLSETLVRFSMLAGRIKGDLIHCS--DEGVGALFVEATV-TVTMADFLKT 120
           KE+   RLK+SLS+TL  F +L G+IKG+LIHCS  D+ VGALFVEA V  +TM +FLK 
Sbjct: 61  KENTATRLKISLSKTLAHFPILTGKIKGNLIHCSNEDDEVGALFVEAKVQKITMGNFLKN 120

Query: 121 PDLQLLAQFLPVPLVPNEPLETFVQVAAQVNFFSCGRIAIGFSFFHKIIDGTTMSAFIKS 180
           P+LQ LAQFLP P  PNEPLE  +Q+ AQV+FFSCG +AIGFSFFHKIIDGTTM AFIKS
Sbjct: 121 PNLQFLAQFLPFPAAPNEPLERTIQIGAQVSFFSCGGLAIGFSFFHKIIDGTTMRAFIKS 180

Query: 181 WAATN---EGTLEKLECGNYS-SASSLFPPREISTSYRLISQETPFAVPQGKSSTTRRFL 240
            AA     EG  + L C  Y  S SSLFP REIS  Y L S +TPF V QGK+ST RRF+
Sbjct: 181 LAAAASNFEGISKNLVCEGYQYSTSSLFPAREISADYNLFSSKTPFVVAQGKNSTCRRFV 240

Query: 241 FDAMAISNLKAKTRSAHVSNPTSVEAVTCFIWKYVMEASRYRSFGE 273
           FD+MAISNLKA+ +S  V NPT+VE VTC IWKY M+ASR R   +
Sbjct: 241 FDSMAISNLKARAKSTRVLNPTTVEVVTCIIWKYAMKASRRRRLSD 286

BLAST of Sgr012117 vs. NCBI nr
Match: XP_022149427.1 (BAHD acyltransferase At5g47980-like isoform X1 [Momordica charantia])

HSP 1 Score: 312.8 bits (800), Expect = 3.2e-81
Identity = 171/284 (60.21%), Postives = 209/284 (73.59%), Query Frame = 0

Query: 1   MEVQIISREVIKPAI--PSDHLKTCQVSLLDM-LSPQFYTPITFFYPKKEIGGHLNKEDA 60
           MEV+IISRE+I+ A        KT  +SLLD+ +  Q YTPI  FY  K+   + N E+ 
Sbjct: 1   MEVEIISREIIRSAAFQRDQQQKTFPLSLLDIYIGGQTYTPIILFYSTKKNQSN-NNENT 60

Query: 61  TARLKLSLSETLVRFSMLAGRIKGDLIHCSDEGVGALFVEATVTVTMADFLKTPDLQLLA 120
            + LKLSLS+TL RF MLAGRIKG+ +       GALFVEA+V  +MADFLK P LQ LA
Sbjct: 61  ISSLKLSLSKTLARFPMLAGRIKGNFVDYCSYNNGALFVEASVKASMADFLKNPVLQFLA 120

Query: 121 QFLPVP-LVPNEPLETFVQVAAQVNFFSCGR-IAIGFSFFHKIIDGTTMSAFIKSWAATN 180
             +P P +V  EPLET VQVAAQVNFFSCG  IA+GFSF HK+IDG TMSAFIK+WAA+N
Sbjct: 121 NLIPFPDIVLKEPLETAVQVAAQVNFFSCGTGIALGFSFLHKMIDGATMSAFIKTWAASN 180

Query: 181 EGTLEKLECGNYSSASSLFPPREISTSYRLISQETPFAVPQGKSSTTRRFLFDAMAISNL 240
           EGTLEKL CG+Y ++SSLFPPREIS+ Y LIS ETPF V QGK S++RRF+FDAMA+SNL
Sbjct: 181 EGTLEKLACGSYEASSSLFPPREISSGYNLISSETPFLVAQGKQSSSRRFVFDAMAVSNL 240

Query: 241 KAKTRSAHVSNPTSVEAVTCFIWKYVMEASRYRSFGESKSILNH 280
           K + +S  V NPTSVEAVTCFIWKY M+A+      ++K ++ +
Sbjct: 241 KVRAKSQSVPNPTSVEAVTCFIWKYAMKATSTCKSLDNKEVVGN 283

BLAST of Sgr012117 vs. NCBI nr
Match: XP_022149406.1 (BAHD acyltransferase At5g47980-like isoform X2 [Momordica charantia])

HSP 1 Score: 303.5 bits (776), Expect = 1.9e-78
Identity = 165/274 (60.22%), Postives = 202/274 (73.72%), Query Frame = 0

Query: 1   MEVQIISREVIKPAI------PSDHLKTCQVSLLDMLSPQFYTPITFFYPKKEIGGHLNK 60
           MEV+IISRE+I PA          H KT Q+SLLD+ + + Y+P   FY  K+     N 
Sbjct: 1   MEVEIISREIIMPAAFQRNHHHYQHQKTFQLSLLDIYASETYSPFILFYNTKK-DQRSNN 60

Query: 61  EDATARLKLSLSETLVRFSMLAGRIKGDLIHCSDEGVGALFVEATVTVTMADFLKTPDLQ 120
           E+A + LKLSLS+TL RF MLAGRIKG+ +H      GALFVEA+V  +MADFL+TP+LQ
Sbjct: 61  ENAISSLKLSLSKTLARFPMLAGRIKGNFVHYCSSNNGALFVEASVKTSMADFLRTPNLQ 120

Query: 121 LLAQFLPVP-LVPNEPLETFVQVAAQVNFFSCGR-IAIGFSFFHKIIDGTTMSAFIKSWA 180
            LA  +P P LV  +PLET VQVAAQVNFFSCG  IA+GFSF HK+IDG TMSAFIK+WA
Sbjct: 121 FLADLIPFPDLVLKQPLETAVQVAAQVNFFSCGTGIALGFSFLHKMIDGATMSAFIKTWA 180

Query: 181 ATNEGTLEKLECGNYSSASSLFPPREISTSYR-LISQETPFAVPQGKSSTTRRFLFDAMA 240
           A+NEGTL+KL CG+Y ++SSLFP REIS++Y  L+  ET   V Q K  + RRF+FDAMA
Sbjct: 181 ASNEGTLDKLACGSYKASSSLFPAREISSNYNDLVFPETALLVAQPKHCSVRRFVFDAMA 240

Query: 241 ISNLKAKTRSAHVSNPTSVEAVTCFIWKYVMEAS 266
           +SNLK + +S  VSNPTSVEAVTCFIWKY M+A+
Sbjct: 241 VSNLKVRAKSQSVSNPTSVEAVTCFIWKYAMKAT 273

BLAST of Sgr012117 vs. ExPASy Swiss-Prot
Match: Q94FT4 (Salutaridinol 7-O-acetyltransferase OS=Papaver somniferum OX=3469 GN=SALAT PE=1 SV=1)

HSP 1 Score: 136.0 bits (341), Expect = 7.0e-31
Identity = 108/287 (37.63%), Postives = 149/287 (51.92%), Query Frame = 0

Query: 3   VQIISREVIKPAIPS-DHLKTCQVSLLDMLSP-QFYTPITFFYPKKEIG--GHLNKEDAT 62
           V++IS+E IKP  P+   LK   +SLLD   P  +Y PI  FYP       G  N  D  
Sbjct: 9   VEVISKETIKPTTPTPSQLKNFNLSLLDQCFPLYYYVPIILFYPATAANSTGSSNHHDDL 68

Query: 63  ARLKLSLSETLVRFSMLAGR-IKGDLIHCSDEGVGALFVEATVTVTMADFLKTPDLQLLA 122
             LK SLS+TLV F  +AGR I   L+ C D+G+   F +  +   M +F+  PD+  L+
Sbjct: 69  DLLKSSLSKTLVHFYPMAGRMIDNILVDCHDQGIN--FYKVKIRGKMCEFMSQPDVP-LS 128

Query: 123 QFLPVPLV-PNEPLETFVQVAAQVNFFSCGRIAIGFSFFHKIIDGTTMSAFIKSWAATNE 182
           Q LP  +V  + P E  V V  QVN F CG  AI  S  HKI D  TMS FI+SWA+T +
Sbjct: 129 QLLPSEVVSASVPKEALVIV--QVNMFDCGGTAICSSVSHKIADAATMSTFIRSWASTTK 188

Query: 183 ---------GTLEKLECGNYSSASSLFPPREISTSYRLISQETPFAV----PQGKSSTTR 242
                       ++    ++ SA SLFPP E  TS   +S E PF+      +   + ++
Sbjct: 189 TSRSGGSTAAVTDQKLIPSFDSA-SLFPPSERLTSPSGMS-EIPFSSTPEDTEDDKTVSK 248

Query: 243 RFLFDAMAISNLKAKTRSAHVSN-----PTSVEAVTCFIWKYVMEAS 266
           RF+FD   I++++ K +     N      T VE VT  IWK VM+++
Sbjct: 249 RFVFDFAKITSVREKLQVLMHDNYKSRRQTRVEVVTSLIWKSVMKST 288

BLAST of Sgr012117 vs. ExPASy Swiss-Prot
Match: Q9FI40 (BAHD acyltransferase At5g47980 OS=Arabidopsis thaliana OX=3702 GN=BAHD1 PE=2 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 8.6e-29
Identity = 87/270 (32.22%), Postives = 145/270 (53.70%), Query Frame = 0

Query: 1   MEVQIISREVIKPAIPSDHLKTCQVSLLDMLSPQFYTPITFFYPKKEIGGHLNKEDATAR 60
           + +++I REVIKP+ P+ H    Q+S++D    +   P+ FFY   ++    + +  + R
Sbjct: 3   LNLEVIQREVIKPSSPAPH-DRLQLSVIDFGIAEACVPMIFFYNLADL-AEKSPDIVSTR 62

Query: 61  LKLSLSETLVRFSMLAGRIKGDLIHCSDEGVGALFVEATVTVTMADFLKTPDLQLLAQFL 120
           L+ SLS+ L RF  LAG+ +G  I C+DE  GA+F EA   + +++FL+  D+  L   +
Sbjct: 63  LRSSLSQALSRFYPLAGKKEGVSISCNDE--GAVFTEARTNLLLSEFLRNIDINSLKILI 122

Query: 121 PVPLVPNEPLETFVQVAAQVNFFSCGR-IAIGFSFFHKIIDGTTMSAFIKSWAATNEG-T 180
           P  L P E L++   ++ Q  FF  G  +A+G    H I D  ++S F++ WAAT  G +
Sbjct: 123 PT-LAPGESLDSRPLLSVQATFFGSGSGLAVGICVSHCICDAASVSTFVRGWAATARGDS 182

Query: 181 LEKLECGNYSSASSLFPPREIS---TSYRLISQETPFAVPQGKSSTTRRFLFDAMAISNL 240
            ++L    ++   ++ PP +IS   + +  +S+         +   T RF+F++  I+ L
Sbjct: 183 NDELSTPQFAEV-AIHPPADISIHGSPFNALSEVR-------EKCVTNRFVFESDKITKL 242

Query: 241 KAKTRSAHVSNPTSVEAVTCFIWKYVMEAS 266
           K    S  V +PT VEAV   IW+    AS
Sbjct: 243 KIVAASKSVPSPTRVEAVMSLIWRCARNAS 259

BLAST of Sgr012117 vs. ExPASy Swiss-Prot
Match: O23393 (BAHD acyltransferase BIA1 OS=Arabidopsis thaliana OX=3702 GN=BIA1 PE=2 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 5.5e-28
Identity = 92/271 (33.95%), Postives = 136/271 (50.18%), Query Frame = 0

Query: 2   EVQIISREVIKPAIPSDHLKTCQVSLLDMLSPQFYTPITFFYPKKEIGGHLNKEDATARL 61
           ++++  +EVIKPA PS      Q+S+LD+  P  Y    FFY        +  E+    L
Sbjct: 4   KLEVTGKEVIKPASPSPR-DRLQLSILDLYCPGIYVSTIFFYDLITESSEVFSEN----L 63

Query: 62  KLSLSETLVRFSMLAGRIKGDLIHCSDEGVGALFVEATVTVTMADFLKTPDLQLLAQFLP 121
           KLSLSETL RF  LAGRI+G  I C+DE  GA+F EA   + + DFL+  +   L+ FLP
Sbjct: 64  KLSLSETLSRFYPLAGRIEGLSISCNDE--GAVFTEARTDLLLPDFLRNLNTDSLSGFLP 123

Query: 122 VPLVPNEPLETFVQVAAQVNFFSCGR-IAIGFSFFHKIIDGTTMSAFIKSWAAT-----N 181
             L   E    +  ++ +V FF  G  +A+  S  HKI D  ++  F+K WA T     +
Sbjct: 124 T-LAAGESPAAWPLLSVKVTFFGSGSGVAVSVSVSHKICDIASLVTFVKDWATTTAKGKS 183

Query: 182 EGTLEKLECGNYSSASSLFPPREISTSYRLISQETPFAVPQGKSSTTRRFLFDAMAISNL 241
             T+E  E       ++++PP       +  S ++   +        +RF+F+   I+ L
Sbjct: 184 NSTIEFAE-------TTIYPPPPSHMYEQFPSTDSDSNIT--SKYVLKRFVFEPSKIAEL 243

Query: 242 KAKTRSAHVSNPTSVEAVTCFIWKYVMEASR 267
           K K  S  V  PT VEA+   IW+    +SR
Sbjct: 244 KHKAASESVPVPTRVEAIMSLIWRCARNSSR 257

BLAST of Sgr012117 vs. ExPASy Swiss-Prot
Match: Q8GZU0 (Minovincinine 19-hydroxy-O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=MAT PE=1 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 2.8e-19
Identity = 87/277 (31.41%), Postives = 135/277 (48.74%), Query Frame = 0

Query: 1   MEVQIISREVIKPAIPS-DHLKTCQVSLLDMLSPQFYTPITFFYPKKEIGGHLNKEDATA 60
           +E + +S+ +IKP+ P+   L    +S  D      Y    FFY   +  GH   E +T 
Sbjct: 7   VETETLSKTLIKPSSPTPQSLSHYNLSYNDQNIYPEYIFAGFFYSNPD--GH---EISTI 66

Query: 61  R--LKLSLSETLVRFSMLAGR-IKGDLIHCSDEGVGALFVEATVTVTMADFLKTPDLQLL 120
           R  L+ SLS+TLV +   AG+ +K D IHC+D+G+   FV+  +   M D LK P+L+  
Sbjct: 67  REQLQNSLSKTLVSYYPFAGKVVKNDYIHCNDDGIE--FVDVRIHCRMNDILK-PELRSY 126

Query: 121 AQFLPVP---LVPNEPLETFVQVAAQVNFFSCGRIAIGFSFFHKIIDGTTMSAFIKSWAA 180
           A  L  P    V +E     V    Q++ F CG +A+ F   HK+ D  T+ +FIK WAA
Sbjct: 127 ASELIRPNRSTVGSEDSTALV----QLSHFDCGGVAVAFGISHKVADAATILSFIKDWAA 186

Query: 181 TNEGTLEKLECGNYSS----ASSLFPPREISTSYRLISQETPFAVPQGKSSTTRRFLFDA 240
           +   T +     + S+    + S+FP ++      +I  +     P   +   +RFLF  
Sbjct: 187 S---TCDLSSSHDVSTPVLVSDSIFPRQD-----NIICGQ----FPASPNCVRKRFLFSP 246

Query: 241 MAISNLKAKTRSAHVSNPTSVEAVTCFIWKYVMEASR 267
            AI  LK+K     +  PT VE +T F+ +    A +
Sbjct: 247 EAIERLKSKAIEFGIEKPTRVEVLTAFLCRCATVAGK 259

BLAST of Sgr012117 vs. ExPASy Swiss-Prot
Match: Q9ZTK5 (Deacetylvindoline O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=DAT PE=1 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 3.6e-19
Identity = 77/261 (29.50%), Postives = 124/261 (47.51%), Query Frame = 0

Query: 1   MEVQIISREVIKPAIPS-DHLKTCQVSLLDMLSPQFYTPITFFYPKKEIGGHLNKEDATA 60
           +E + +S+ +IKP+ P+   L    +S  D    Q    + FFY   +    +       
Sbjct: 8   VETETLSKTLIKPSSPTPQSLSRYNLSYNDQNIYQTCVSVGFFYENPD---GIEISTIRE 67

Query: 61  RLKLSLSETLVRFSMLAGR-IKGDLIHCSDEGVGALFVEATVTVTMADFLKTPDLQLLAQ 120
           +L+ SLS+TLV +   AG+ +K D IHC+D+G+   FVE  +   M D LK  +L+  A+
Sbjct: 68  QLQNSLSKTLVSYYPFAGKVVKNDYIHCNDDGIE--FVEVRIRCRMNDILKY-ELRSYAR 127

Query: 121 FLPVPLVPNEPLETFVQVAAQVNFFSCGRIAIGFSFFHKIIDGTTMSAFIKSWAATNEGT 180
            L +P       E    +  Q++ F CG +A+ F   HK+ DG T+++F+K WAA+    
Sbjct: 128 DLVLPKRVTVGSEDTTAI-VQLSHFDCGGLAVAFGISHKVADGGTIASFMKDWAAS---- 187

Query: 181 LEKLECGNYSSASSLFPPREISTSYRLISQETPFA--VPQGKSSTTRRFLFDAMAISNLK 240
                   Y S+S   P   + +      Q+       P  K+   + F+F   AI  LK
Sbjct: 188 ------ACYLSSSHHVPTPLLVSDSIFPRQDNIICEQFPTSKNCVEKTFIFPPEAIEKLK 247

Query: 241 AKTRSAHVSNPTSVEAVTCFI 258
           +K     +  PT VE +T F+
Sbjct: 248 SKAVEFGIEKPTRVEVLTAFL 251

BLAST of Sgr012117 vs. ExPASy TrEMBL
Match: A0A6J1C8U3 (vinorine synthase-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111009443 PE=3 SV=1)

HSP 1 Score: 317.8 bits (813), Expect = 4.8e-83
Identity = 170/280 (60.71%), Postives = 209/280 (74.64%), Query Frame = 0

Query: 1   MEVQIISREVIKPAIP-SDHLKTCQVSLLDMLSPQFYTPITFFYPKKEIGGHLNKEDATA 60
           ME++IISRE+IKP +P ++  KT +VS+LD+LSP  YTPITFFY       H    +AT+
Sbjct: 1   MELEIISREIIKPPMPLNNDFKTFKVSMLDILSPDSYTPITFFYSMNNNQQH--SHNATS 60

Query: 61  RLKLSLSETLVRFSMLAGRIKGDLIHCS-DEGVGALFVEATVTVTMADFLKTPDLQLLAQ 120
           RLKLSLS+ L RF M+AG+ KGD IHCS +E VGALFVEA V ++MA+FLK P+LQLLA 
Sbjct: 61  RLKLSLSQALARFPMVAGKFKGDSIHCSNEEDVGALFVEAEVKMSMAEFLKAPNLQLLAN 120

Query: 121 FLPVPLVPNEPLETFVQVAAQVNFFSCGRIAIGFSFFHKIIDGTTMSAFIKSWAATNEGT 180
           FLP+  +PN PLE  VQ+A QVN F CG IAIG SF HKIIDGTTMSAF++SWAA+ EG 
Sbjct: 121 FLPL-FIPNGPLEKAVQIAVQVNVFECGGIAIGASFLHKIIDGTTMSAFLRSWAASCEGK 180

Query: 181 LEKLECGNYSSASSLFPPREISTSYRLISQETPFAVPQGKSSTTRRFLFDAMAISNLKAK 240
           + KL CGN++  SSLFP REI   Y L S   PF+V QGKSST RRF+FDAMAI+NLK +
Sbjct: 181 MGKLVCGNFNWGSSLFPTREIWDGYNLFS---PFSVKQGKSSTARRFVFDAMAIANLKGR 240

Query: 241 TRSAHVSNPTSVEAVTCFIWKYVMEASRYRSFGESKSILN 279
            +S  V NPTSVE VTCFIWKY ++ASR  +  + K  ++
Sbjct: 241 AKSTSVPNPTSVEVVTCFIWKYAIKASRSCTSTDKKEFVD 274

BLAST of Sgr012117 vs. ExPASy TrEMBL
Match: A0A6J1C9H0 (vinorine synthase-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111009443 PE=3 SV=1)

HSP 1 Score: 317.0 bits (811), Expect = 8.2e-83
Identity = 169/268 (63.06%), Postives = 204/268 (76.12%), Query Frame = 0

Query: 1   MEVQIISREVIKPAIP-SDHLKTCQVSLLDMLSPQFYTPITFFYPKKEIGGHLNKEDATA 60
           ME++IISRE+IKP +P ++  KT +VS+LD+LSP  YTPITFFY       H    +AT+
Sbjct: 1   MELEIISREIIKPPMPLNNDFKTFKVSMLDILSPDSYTPITFFYSMNNNQQH--SHNATS 60

Query: 61  RLKLSLSETLVRFSMLAGRIKGDLIHCS-DEGVGALFVEATVTVTMADFLKTPDLQLLAQ 120
           RLKLSLS+ L RF M+AG+ KGD IHCS +E VGALFVEA V ++MA+FLK P+LQLLA 
Sbjct: 61  RLKLSLSQALARFPMVAGKFKGDSIHCSNEEDVGALFVEAEVKMSMAEFLKAPNLQLLAN 120

Query: 121 FLPVPLVPNEPLETFVQVAAQVNFFSCGRIAIGFSFFHKIIDGTTMSAFIKSWAATNEGT 180
           FLP+  +PN PLE  VQ+A QVN F CG IAIG SF HKIIDGTTMSAF++SWAA+ EG 
Sbjct: 121 FLPL-FIPNGPLEKAVQIAVQVNVFECGGIAIGASFLHKIIDGTTMSAFLRSWAASCEGK 180

Query: 181 LEKLECGNYSSASSLFPPREISTSYRLISQETPFAVPQGKSSTTRRFLFDAMAISNLKAK 240
           + KL CGN++  SSLFP REI   Y L S   PF+V QGKSST RRF+FDAMAI+NLK +
Sbjct: 181 MGKLVCGNFNWGSSLFPTREIWDGYNLFS---PFSVKQGKSSTARRFVFDAMAIANLKGR 240

Query: 241 TRSAHVSNPTSVEAVTCFIWKYVMEASR 267
            +S  V NPTSVE VTCFIWKY ++ASR
Sbjct: 241 AKSTSVPNPTSVEVVTCFIWKYAIKASR 262

BLAST of Sgr012117 vs. ExPASy TrEMBL
Match: A0A6J1C8E0 (BAHD acyltransferase BIA1-like OS=Momordica charantia OX=3673 GN=LOC111009175 PE=3 SV=1)

HSP 1 Score: 316.2 bits (809), Expect = 1.4e-82
Identity = 175/286 (61.19%), Postives = 205/286 (71.68%), Query Frame = 0

Query: 1   MEVQIISREVIKPA----IPSDHLKTCQVSLLDMLSPQFYTPITFFYPKKEIGGHLN--- 60
           MEV+IISRE+IKPA    +P D  KT QVSLLD +SP  Y PI FFY  KE   + +   
Sbjct: 1   MEVEIISREIIKPATLEEMPQDDPKTFQVSLLDKVSPHAYIPIIFFYSMKEETDNSHIFF 60

Query: 61  KEDATARLKLSLSETLVRFSMLAGRIKGDLIHCS--DEGVGALFVEATV-TVTMADFLKT 120
           KE+   RLK+SLS+TL  F +L G+IKG+LIHCS  D+ VGALFVEA V  +TM +FLK 
Sbjct: 61  KENTATRLKISLSKTLAHFPILTGKIKGNLIHCSNEDDEVGALFVEAKVQKITMGNFLKN 120

Query: 121 PDLQLLAQFLPVPLVPNEPLETFVQVAAQVNFFSCGRIAIGFSFFHKIIDGTTMSAFIKS 180
           P+LQ LAQFLP P  PNEPLE  +Q+ AQV+FFSCG +AIGFSFFHKIIDGTTM AFIKS
Sbjct: 121 PNLQFLAQFLPFPAAPNEPLERTIQIGAQVSFFSCGGLAIGFSFFHKIIDGTTMRAFIKS 180

Query: 181 WAATN---EGTLEKLECGNYS-SASSLFPPREISTSYRLISQETPFAVPQGKSSTTRRFL 240
            AA     EG  + L C  Y  S SSLFP REIS  Y L S +TPF V QGK+ST RRF+
Sbjct: 181 LAAAASNFEGISKNLVCEGYQYSTSSLFPAREISADYNLFSSKTPFVVAQGKNSTCRRFV 240

Query: 241 FDAMAISNLKAKTRSAHVSNPTSVEAVTCFIWKYVMEASRYRSFGE 273
           FD+MAISNLKA+ +S  V NPT+VE VTC IWKY M+ASR R   +
Sbjct: 241 FDSMAISNLKARAKSTRVLNPTTVEVVTCIIWKYAMKASRRRRLSD 286

BLAST of Sgr012117 vs. ExPASy TrEMBL
Match: A0A6J1D7X5 (BAHD acyltransferase At5g47980-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111017856 PE=3 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 1.5e-81
Identity = 171/284 (60.21%), Postives = 209/284 (73.59%), Query Frame = 0

Query: 1   MEVQIISREVIKPAI--PSDHLKTCQVSLLDM-LSPQFYTPITFFYPKKEIGGHLNKEDA 60
           MEV+IISRE+I+ A        KT  +SLLD+ +  Q YTPI  FY  K+   + N E+ 
Sbjct: 1   MEVEIISREIIRSAAFQRDQQQKTFPLSLLDIYIGGQTYTPIILFYSTKKNQSN-NNENT 60

Query: 61  TARLKLSLSETLVRFSMLAGRIKGDLIHCSDEGVGALFVEATVTVTMADFLKTPDLQLLA 120
            + LKLSLS+TL RF MLAGRIKG+ +       GALFVEA+V  +MADFLK P LQ LA
Sbjct: 61  ISSLKLSLSKTLARFPMLAGRIKGNFVDYCSYNNGALFVEASVKASMADFLKNPVLQFLA 120

Query: 121 QFLPVP-LVPNEPLETFVQVAAQVNFFSCGR-IAIGFSFFHKIIDGTTMSAFIKSWAATN 180
             +P P +V  EPLET VQVAAQVNFFSCG  IA+GFSF HK+IDG TMSAFIK+WAA+N
Sbjct: 121 NLIPFPDIVLKEPLETAVQVAAQVNFFSCGTGIALGFSFLHKMIDGATMSAFIKTWAASN 180

Query: 181 EGTLEKLECGNYSSASSLFPPREISTSYRLISQETPFAVPQGKSSTTRRFLFDAMAISNL 240
           EGTLEKL CG+Y ++SSLFPPREIS+ Y LIS ETPF V QGK S++RRF+FDAMA+SNL
Sbjct: 181 EGTLEKLACGSYEASSSLFPPREISSGYNLISSETPFLVAQGKQSSSRRFVFDAMAVSNL 240

Query: 241 KAKTRSAHVSNPTSVEAVTCFIWKYVMEASRYRSFGESKSILNH 280
           K + +S  V NPTSVEAVTCFIWKY M+A+      ++K ++ +
Sbjct: 241 KVRAKSQSVPNPTSVEAVTCFIWKYAMKATSTCKSLDNKEVVGN 283

BLAST of Sgr012117 vs. ExPASy TrEMBL
Match: A0A6J1D899 (BAHD acyltransferase At5g47980-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111017835 PE=3 SV=1)

HSP 1 Score: 303.5 bits (776), Expect = 9.4e-79
Identity = 165/274 (60.22%), Postives = 202/274 (73.72%), Query Frame = 0

Query: 1   MEVQIISREVIKPAI------PSDHLKTCQVSLLDMLSPQFYTPITFFYPKKEIGGHLNK 60
           MEV+IISRE+I PA          H KT Q+SLLD+ + + Y+P   FY  K+     N 
Sbjct: 1   MEVEIISREIIMPAAFQRNHHHYQHQKTFQLSLLDIYASETYSPFILFYNTKK-DQRSNN 60

Query: 61  EDATARLKLSLSETLVRFSMLAGRIKGDLIHCSDEGVGALFVEATVTVTMADFLKTPDLQ 120
           E+A + LKLSLS+TL RF MLAGRIKG+ +H      GALFVEA+V  +MADFL+TP+LQ
Sbjct: 61  ENAISSLKLSLSKTLARFPMLAGRIKGNFVHYCSSNNGALFVEASVKTSMADFLRTPNLQ 120

Query: 121 LLAQFLPVP-LVPNEPLETFVQVAAQVNFFSCGR-IAIGFSFFHKIIDGTTMSAFIKSWA 180
            LA  +P P LV  +PLET VQVAAQVNFFSCG  IA+GFSF HK+IDG TMSAFIK+WA
Sbjct: 121 FLADLIPFPDLVLKQPLETAVQVAAQVNFFSCGTGIALGFSFLHKMIDGATMSAFIKTWA 180

Query: 181 ATNEGTLEKLECGNYSSASSLFPPREISTSYR-LISQETPFAVPQGKSSTTRRFLFDAMA 240
           A+NEGTL+KL CG+Y ++SSLFP REIS++Y  L+  ET   V Q K  + RRF+FDAMA
Sbjct: 181 ASNEGTLDKLACGSYKASSSLFPAREISSNYNDLVFPETALLVAQPKHCSVRRFVFDAMA 240

Query: 241 ISNLKAKTRSAHVSNPTSVEAVTCFIWKYVMEAS 266
           +SNLK + +S  VSNPTSVEAVTCFIWKY M+A+
Sbjct: 241 VSNLKVRAKSQSVSNPTSVEAVTCFIWKYAMKAT 273

BLAST of Sgr012117 vs. TAIR 10
Match: AT4G15390.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 161.8 bits (408), Expect = 8.5e-40
Identity = 103/273 (37.73%), Postives = 160/273 (58.61%), Query Frame = 0

Query: 1   MEVQIISREVIKPAIPS-DHLKTCQVSLLDMLSPQFYTPITFFYPKKEIGGHLNKEDATA 60
           M+V+ IS+E+IKP+ P+ ++L+T Q+S+ D + P  YT    FY K ++   +++E  + 
Sbjct: 6   MKVETISKEIIKPSSPTPNNLQTLQLSIYDHILPPVYTVAFLFYTKNDL---ISQEHTSH 65

Query: 61  RLKLSLSETLVRFSMLAGRIKGDLIHCSDEGVGALFVEATV-TVTMADFLKTPDLQLLAQ 120
           +LK SLSETL +F  LAGRI G  + C+DE  GA+FV+A V    + +FLK PD   L Q
Sbjct: 66  KLKTSLSETLTKFYPLAGRITGVTVDCTDE--GAIFVDARVNNCPLTEFLKCPDFDALQQ 125

Query: 121 FLPVPLVPNE--PLETFVQVAAQVNFFSCGRIAIGFSFFHKIIDGTTMSAFIKSWAAT-- 180
            LP+ +V N      T+  +  +  +F CG +AIG    HKI D  ++S FI+SWAAT  
Sbjct: 126 LLPLDVVDNPYVAAATWPLLLVKATYFGCGGMAIGICITHKIADAASISTFIRSWAATAR 185

Query: 181 NEGTLEKLECGNYSSASSLFPPREISTSYRLISQETPFAVPQGKSSTTRRFLFDAMAISN 240
            E     +E   ++ A + +PP   + +++L + E        +SS T+RF+F+A  + +
Sbjct: 186 GENDAAAMESPVFAGA-NFYPP--ANEAFKLPADEQ----AGKRSSITKRFVFEASKVED 245

Query: 241 LKAKTRSAH-VSNPTSVEAVTCFIWKYVMEASR 267
           L+ K  S   V  PT VE+VT  IWK  + +S+
Sbjct: 246 LRTKAASEETVDQPTRVESVTALIWKCFVASSK 266

BLAST of Sgr012117 vs. TAIR 10
Match: AT3G26040.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 158.7 bits (400), Expect = 7.2e-39
Identity = 105/294 (35.71%), Postives = 164/294 (55.78%), Query Frame = 0

Query: 1   MEVQIISREVIKPAIPS-DHLKTCQVSLLDMLSPQFYTPITFFYPKKEIGGHLNKEDATA 60
           M V ++SR++IKP+ P+ +HLK  ++SLL+ L P  + P+ FFY        +   +   
Sbjct: 1   MRVDVVSRDIIKPSSPTPNHLKKFKLSLLEQLGPTIFGPMVFFY---SANNSIKPTEQLQ 60

Query: 61  RLKLSLSETLVRFSMLAGRIKGDL-IHCSDEGVGALFVEATVTVTMADFLKTPDLQLLAQ 120
            LK SLSETL  F  LAGR+KG++ I C+D   GA F+EA V   +++ L  P    L Q
Sbjct: 61  MLKKSLSETLTHFYPLAGRLKGNISIDCNDS--GADFLEARVNSPLSNLLLEPSSDSLQQ 120

Query: 121 FLPVPLVPNEPLETFVQ-VAAQVNFFSCGRIAIGFSFFHKIIDGTTMSAFIKSWAA-TNE 180
            +P  +   + +ET  + + AQ +FF CG ++IG    HK+ D T++  F+KSWAA ++ 
Sbjct: 121 LIPTSV---DSIETRTRLLLAQASFFECGSMSIGVCISHKLADATSIGLFMKSWAAISSR 180

Query: 181 GTLEKLECGNYSSASSLFPPREISTSYRLISQETPFAVPQGKSSTT--RRFLFDAMAISN 240
           G+++ +    + +   +FPP   S      +   P   P+   + T  +RF+FD+ +I  
Sbjct: 181 GSIKTIGAPVFDTV-KIFPPGNFSE-----TSPAPVVEPEIMMNQTLSKRFIFDSSSIQA 240

Query: 241 LKAKTRSAHVSNPTSVEAVTCFIWKYVMEASRYRSFGESKSILNHPNMLFSHIN 289
           L+AK  S  V+ PT VEAV+  IWK  M+A+R  S     SIL +   L S ++
Sbjct: 241 LQAKASSFEVNQPTRVEAVSALIWKSAMKATRTVSGTSKPSILANSVSLRSRVS 280

BLAST of Sgr012117 vs. TAIR 10
Match: AT3G30280.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 157.1 bits (396), Expect = 2.1e-38
Identity = 103/289 (35.64%), Postives = 163/289 (56.40%), Query Frame = 0

Query: 1   MEVQIISREVIKPA--IPSDHLKTCQVSLLDMLSPQFYTPITFFYPKKEIGGHLNKEDAT 60
           M+V+ I++E+IKP+   P+D L+T Q+S+ D + P  YT    FY K ++   ++ E ++
Sbjct: 4   MKVETIAKEIIKPSSTTPND-LQTLQLSIYDHILPPVYTVAFLFYTKDDL---ISPEQSS 63

Query: 61  ARLKLSLSETLVRFSMLAGRIKGDLIHCSDEGVGALFVEATV-TVTMADFLKTPDLQLLA 120
            +LK SL+ETL +F  LAGRIKG  I C+DE  GA+FV+A V    ++DFL++PD + L 
Sbjct: 64  HKLKTSLAETLTKFYPLAGRIKGVTIDCNDE--GAVFVDARVNNYPLSDFLRSPDFKTLQ 123

Query: 121 QFLPVPLV--PNEPLETFVQVAAQVNFFSCGRIAIGFSFFHKIIDGTTMSAFIKSWAATN 180
           Q LP+ ++  P E   T+  +  +  +F CG +AIG    HKI D T++S FI+SWAA  
Sbjct: 124 QLLPLDVIDDPYEAATTWPLLLVKATYFPCGGMAIGLCITHKIADATSISTFIQSWAAMA 183

Query: 181 EGTLEKLECGNYSSASSLFPPREISTSYRLISQETPFAVPQGKSSTTRRFLFDAMAISNL 240
            G    +      +A++ +PP   +  ++    E    +    S  T+RF+F A  +  L
Sbjct: 184 RGEAGDIVADPVFAAANFYPP--ANELFKFPVDENANKI----SQITKRFVFSASKLEEL 243

Query: 241 KAKTRSA-HVSNPTSVEAVTCFIWK-YVMEASRYRSFGESKSILNHPNM 283
           + K  S   V  P  VE+VT  +WK +V  AS   +  + K ++   N+
Sbjct: 244 RTKAASEDFVGRPKRVESVTALLWKAFVAAASSNTNACDMKVLIQPSNL 280

BLAST of Sgr012117 vs. TAIR 10
Match: AT1G24420.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 155.2 bits (391), Expect = 7.9e-38
Identity = 106/280 (37.86%), Postives = 154/280 (55.00%), Query Frame = 0

Query: 3   VQIISREVIKPAIPS-DHLKTCQVSLLDMLSPQFYTPITFFY---PKKEIGGHLNKEDAT 62
           V+I+SRE++KP+ P+ D  +   +SLLD+LS   YT    FY   P+  +G   + E+ +
Sbjct: 5   VEILSREIVKPSSPTPDDKRILNLSLLDILSSPMYTGALLFYAADPQNLLG--FSTEETS 64

Query: 63  ARLKLSLSETLVRFSMLAGRIKGDLIHCSDEGVGALFVEATVTVTMADFLKTPDLQLLAQ 122
            +LK SLS+TL  F  LAGRI G  + C+DE  GA+F+EA V   +++FLK P  + L  
Sbjct: 65  LKLKKSLSKTLPIFYPLAGRIIGSFVECNDE--GAVFIEARVDHLLSEFLKCPVPESLEL 124

Query: 123 FLPVPLVPNEPLETFVQVAAQVNFFSCGRIAIGFSFFHKIIDGTTMSAFIKSWAATNEGT 182
            +PV     E + T+  +  Q NFFSCG + I     HKI D T+++ FI+ WA ++ G 
Sbjct: 125 LIPVEAKSREAV-TWPVLLIQANFFSCGGLVITICVSHKITDATSLAMFIRGWAESSRGL 184

Query: 183 LEKLECGNYSSASSLFP-PREISTSYRLISQETPFAVPQGKSSTTRRFLFDAMAISNLKA 242
              +      +AS +FP P +   S  +  +E      +  S  T+RF+FDA  I  L+A
Sbjct: 185 --GITLIPSFTASEVFPKPLDELPSKPMDRKEE----VEEMSCVTKRFVFDASKIKKLRA 244

Query: 243 KTRSAHVSNPTSVEAVTCFIWKYVMEASRYRSFGESKSIL 278
           K     V NPT VEAVT   W+ V + SR  S     S+L
Sbjct: 245 KASRNLVKNPTRVEAVTALFWRCVTKVSRLSSLTPRTSVL 273

BLAST of Sgr012117 vs. TAIR 10
Match: AT5G47950.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 148.3 bits (373), Expect = 9.7e-36
Identity = 98/268 (36.57%), Postives = 152/268 (56.72%), Query Frame = 0

Query: 1   MEVQIISREVIKPA--IPSDHLKTCQVSLLDMLSPQFYTPITFFYPKKEIGGHLNKEDAT 60
           M+V+ I +E+IKP+   P+D L T Q+S++D+L P  Y     FY K ++   +++E  +
Sbjct: 4   MKVETIGKEIIKPSATTPTD-LSTLQLSIMDILMPPVYAVAFLFYTKDDL---ISQEQTS 63

Query: 61  ARLKLSLSETLVRFSMLAGRIKGDLIHCSDEGVGALFVEATV-TVTMADFLKTPDLQLLA 120
             LK SLSE L +F  LAGR+ G  I  +DE  GA+FVEA V    ++ FL++PD + L 
Sbjct: 64  HTLKTSLSEILTKFHPLAGRVNGVTIKSTDE--GAVFVEARVDNCDLSGFLRSPDTESLK 123

Query: 121 QFLPVPLVPNEPLETFVQVAAQVNFFSCGRIAIGFSFFHKIIDGTTMSAFIKSWAATNEG 180
           Q LP   V +EP  T+  +  +  +F CG +AIG    H++ D  ++S F+++WAAT  G
Sbjct: 124 QLLP---VDDEPAPTWPLLLVKATYFRCGGMAIGLCISHRLADAASLSIFLQAWAATARG 183

Query: 181 TLEKLECGNYSSASSLFPPREISTSYRLISQETPFAVPQGKSSTTRRFLFDAMAISNLKA 240
             + +   ++ S + L+P    +        + P  V   ++S T+RF+F A  I  L+ 
Sbjct: 184 ESDLVASPDFCS-TKLYPAANEAI-------KIPGEV-VNRTSVTKRFVFVASKIEELRN 243

Query: 241 KTRSAHVSNPTSVEAVTCFIWKYVMEAS 266
           K  S  V  PT V++VT  IWK V+ AS
Sbjct: 244 KVASDVVPRPTRVQSVTSLIWKCVVTAS 253