Sgr011886 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr011886
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Descriptioncytochrome P450 78A3-like
Locationtig00153113: 163579 .. 165368 (+)
RNA-Seq ExpressionSgr011886
SyntenySgr011886
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCGATGAGTAGTGAAATTGAAAGCCTCTGGGTTTTCGCGCTGGCTTCTAAATGCTCTGCTTTAACTAAAGAAAACATCCTCTGGTCTTTACTCTTCTTTTTCCTAATCTGGGTTTCTGTTTCCATTCTCCACTGGGCCCATCCGGGCGGCCCGGCTTGGGGCCGCTACTGGTGGCGCCGCCGCCGCAGCAATTCCACCGCCGCTGCTATTCCCGGCCCGAGAGGCCTCCCCCTCGTCGGCAGCATGGGCTTGATGGCCGACTTGGCCCACCACCGGATTGCCGCCGTGGCTGACTCCTTAAACGCCACCCGCCTCATGGCCTTTTCGCTCGGCGACACTCGCGTGATCGTCACATGCAACCCCGACGTCGCCAAAGAGATTCTCAACAGCTCCCTCTTCGCCGACCGCCCCGTTAAGGAGTCCGCTTACTCCTTGATGTTCAACCGCGCCATTGGGTTCGCCCCCTACGGCCTTACTGGCGGACCCTCCGCCGCATCGCTTCCCACCACCTCTTCTGCCCCAAGCAAATCAAGTCCTCCCAGTCCCAGCGCCGCCAAATCGCTTCCCAAATGGTCGCAATGTTCGCAAACCGCGATACCACACAGAGCCTCTGCGTTCGCGACTCTCTCAAGCGGGCTTCTCTCAACAACATGATGGGCTCTGTTTTCGGCCGAGTTTACGACCTCTCTGACTCGGCTAACAATGACGTCCAAGAACTCCAGAGCCTCGTCGACGAAGGCTACGACTTGCTGGGCCTCCTCAACTGGTCCGACCACCTCCCATGGCTCGCCGACTTCGACTCTCAGAAAATCCGGTTCAGATGCTCCCGACTCGTCCCCAAGGTGAACCACTTCGTCGGCCGGATCATCGCCGAACACCGCGCCAAATCCGACAACCAAGTCCTAGATTTCGTCGACGTTTTGCTCTCTCTCCAAGAAGCCGACAAACTCTCTGACTCCGATATGATCGCCGTTCTTTGGGTTAGTATTAAATTAATTCTTTTTAATTAATCAATTTCAAGATTAAAATTGCATATATTTTCTAATTTCAAATCAGAATATGTACACGTAGACTAGAAGTTTAATCGTTGGTGAAAGACATTCTTCACTCTTTTTTTTTTTTTTATAATATATGTAATTAATTAAATGATTTCTTTTTTTGTTGTTGTTTTTTTTCGTTTTTGGGGTGTAGGAAATGATTTTTCGTGGGACGGACACGGTGGCAGTTTTAATGAGTGGATACTGGCCAGGATGGTACTTCACAACGATATCCAAAGGAAAGTTCAAGAGGAGCTAGATAACGTGGTTGGGAGTACACGCGCCGTCGCGGAATCCGACATTCCGTCGCTGGTGTATCTAACGGCTGTGGTTAAGGAAGTTCTGAGGTTACATCCGCCGGGCCCACTCCTGTCGTGGGCCCGCCTAGCCATCACTGATACAATCATCGATGGGCATCACGTGCCCCGGGGGACCACCGCTATGGTTAACATGTGGTCGATAGCGCGGGACCCACAGGTCTGGTCGGACCCACTCGAATTTATGCCCCAGAGGTTTGTGTCCGACCCCGGTGACGTGGAGTTCTCGGTCATGGGTTCGGATCTCCGGCTGGCTCCGTTCGGGTCGGGCAGAAGGACCTGCCCCGGAAGGCCTTCGCCTGGACAACTGTCACCTTCTGGGTGGCCACGCTTTTACACGACTTCAAATGGTCGCCGTCCGATCAAAACGACGCCGTCGACTTGTCGAGGTCCTCAAGCTCTCCTGCGAAATGGCCAATCCCCTCACCGTTAA

mRNA sequence

ATGTCGATGAGTAGTGAAATTGAAAGCCTCTGGGTTTTCGCGCTGGCTTCTAAATGCTCTGCTTTAACTAAAGAAAACATCCTCTGGTCTTTACTCTTCTTTTTCCTAATCTGGGTTTCTGTTTCCATTCTCCACTGGGCCCATCCGGGCGGCCCGGCTTGGGGCCGCTACTGGTGGCGCCGCCGCCGCAGCAATTCCACCGCCGCTGCTATTCCCGGCCCGAGAGGCCTCCCCCTCGTCGGCAGCATGGGCTTGATGGCCGACTTGGCCCACCACCGGATTGCCGCCGTGGCTGACTCCTTAAACGCCACCCGCCTCATGGCCTTTTCGCTCGGCGACACTCGCGTGATCGTCACATGCAACCCCGACGTCGCCAAAGAGATTCTCAACAGCTCCCTCTTCGCCGACCGCCCCGTTAAGGAGTCCGCTTACTCCTTGATGTTCAACCGCGCCATTGGGTTCGCCCCCTACGGCCTTACTGGCGGACCCTCCGCCGCATCGCTTCCCACCACCTCTTCTGCCCCAAGCAAATCAAGTCCTCCCAGTCCCAGCGCCGCCAAATCGCTTCCCAAATGGTCGCAATTTTACGACCTCTCTGACTCGGCTAACAATGACGTCCAAGAACTCCAGAGCCTCGTCGACGAAGGCTACGACTTGCTGGGCCTCCTCAACTGGTCCGACCACCTCCCATGGCTCGCCGACTTCGACTCTCAGAAAATCCGGTTCAGATGCTCCCGACTCGTCCCCAAGGTGAACCACTTCGTCGGCCGGATCATCGCCGAACACCGCGCCAAATCCGACAACCAAGTCCTAGATTTCGTCGACGTTTTGCTCTCTCTCCAAGAAGCCGACAAACTCTCTGACTCCGATATGATCGCCGTTCTTTGGGTTAGAAATGATTTTTCGTGGGACGGACACGGTGGCAGTTTTAATGAGTGGATACTGGCCAGGATGGTACTTCACAACGATATCCAAAGGAAAGTTCAAGAGGAGCTAGATAACGTGGTTGGGAGTACACGCGCCGTCGCGGAATCCGACATTCCGTCGCTGGTGTATCTAACGGCTGTGGTTAAGGAAGTTCTGAGGTTACATCCGCCGGGCCCACTCCTGTCGTGGGCCCGCCTAGCCATCACTGATACAATCATCGATGGGCATCACGTGCCCCGGGGGACCACCGCTATGGTTAACATGTGGTCGATAGCGCGGGACCCACAGGTCTGGTCGGACCCACTCGAATTTATGCCCCAGAGGTTTGTGTCCGACCCCGGTGACGTGGAGTTCTCGGTCATGGGTTCGGATCTCCGGCTGGCTCCGTTCGGGTCGGGCAGAAGGACCTGCCCCGGAAGGCCTTCGCCTGGACAACTGTCACCTTCTGGGTGGCCACGCTTTTACACGACTTCAAATGGTCGCCGTCCGATCAAAACGACGCCGTCGACTTGTCGAGGTCCTCAAGCTCTCCTGCGAAATGGCCAATCCCCTCACCGTTAA

Coding sequence (CDS)

ATGTCGATGAGTAGTGAAATTGAAAGCCTCTGGGTTTTCGCGCTGGCTTCTAAATGCTCTGCTTTAACTAAAGAAAACATCCTCTGGTCTTTACTCTTCTTTTTCCTAATCTGGGTTTCTGTTTCCATTCTCCACTGGGCCCATCCGGGCGGCCCGGCTTGGGGCCGCTACTGGTGGCGCCGCCGCCGCAGCAATTCCACCGCCGCTGCTATTCCCGGCCCGAGAGGCCTCCCCCTCGTCGGCAGCATGGGCTTGATGGCCGACTTGGCCCACCACCGGATTGCCGCCGTGGCTGACTCCTTAAACGCCACCCGCCTCATGGCCTTTTCGCTCGGCGACACTCGCGTGATCGTCACATGCAACCCCGACGTCGCCAAAGAGATTCTCAACAGCTCCCTCTTCGCCGACCGCCCCGTTAAGGAGTCCGCTTACTCCTTGATGTTCAACCGCGCCATTGGGTTCGCCCCCTACGGCCTTACTGGCGGACCCTCCGCCGCATCGCTTCCCACCACCTCTTCTGCCCCAAGCAAATCAAGTCCTCCCAGTCCCAGCGCCGCCAAATCGCTTCCCAAATGGTCGCAATTTTACGACCTCTCTGACTCGGCTAACAATGACGTCCAAGAACTCCAGAGCCTCGTCGACGAAGGCTACGACTTGCTGGGCCTCCTCAACTGGTCCGACCACCTCCCATGGCTCGCCGACTTCGACTCTCAGAAAATCCGGTTCAGATGCTCCCGACTCGTCCCCAAGGTGAACCACTTCGTCGGCCGGATCATCGCCGAACACCGCGCCAAATCCGACAACCAAGTCCTAGATTTCGTCGACGTTTTGCTCTCTCTCCAAGAAGCCGACAAACTCTCTGACTCCGATATGATCGCCGTTCTTTGGGTTAGAAATGATTTTTCGTGGGACGGACACGGTGGCAGTTTTAATGAGTGGATACTGGCCAGGATGGTACTTCACAACGATATCCAAAGGAAAGTTCAAGAGGAGCTAGATAACGTGGTTGGGAGTACACGCGCCGTCGCGGAATCCGACATTCCGTCGCTGGTGTATCTAACGGCTGTGGTTAAGGAAGTTCTGAGGTTACATCCGCCGGGCCCACTCCTGTCGTGGGCCCGCCTAGCCATCACTGATACAATCATCGATGGGCATCACGTGCCCCGGGGGACCACCGCTATGGTTAACATGTGGTCGATAGCGCGGGACCCACAGGTCTGGTCGGACCCACTCGAATTTATGCCCCAGAGGTTTGTGTCCGACCCCGGTGACGTGGAGTTCTCGGTCATGGGTTCGGATCTCCGGCTGGCTCCGTTCGGGTCGGGCAGAAGGACCTGCCCCGGAAGGCCTTCGCCTGGACAACTGTCACCTTCTGGGTGGCCACGCTTTTACACGACTTCAAATGGTCGCCGTCCGATCAAAACGACGCCGTCGACTTGTCGAGGTCCTCAAGCTCTCCTGCGAAATGGCCAATCCCCTCACCGTTAA

Protein sequence

MSMSSEIESLWVFALASKCSALTKENILWSLLFFFLIWVSVSILHWAHPGGPAWGRYWWRRRRSNSTAAAIPGPRGLPLVGSMGLMADLAHHRIAAVADSLNATRLMAFSLGDTRVIVTCNPDVAKEILNSSLFADRPVKESAYSLMFNRAIGFAPYGLTGGPSAASLPTTSSAPSKSSPPSPSAAKSLPKWSQFYDLSDSANNDVQELQSLVDEGYDLLGLLNWSDHLPWLADFDSQKIRFRCSRLVPKVNHFVGRIIAEHRAKSDNQVLDFVDVLLSLQEADKLSDSDMIAVLWVRNDFSWDGHGGSFNEWILARMVLHNDIQRKVQEELDNVVGSTRAVAESDIPSLVYLTAVVKEVLRLHPPGPLLSWARLAITDTIIDGHHVPRGTTAMVNMWSIARDPQVWSDPLEFMPQRFVSDPGDVEFSVMGSDLRLAPFGSGRRTCPGRPSPGQLSPSGWPRFYTTSNGRRPIKTTPSTCRGPQALLRNGQSPHR
Homology
BLAST of Sgr011886 vs. NCBI nr
Match: XP_038875232.1 (cytochrome P450 78A3-like [Benincasa hispida])

HSP 1 Score: 602.1 bits (1551), Expect = 4.5e-168
Identity = 322/482 (66.80%), Postives = 370/482 (76.76%), Query Frame = 0

Query: 1   MSMSSEIESLWVFALASKCSALTKENILWSLLFFFLIWVSVSILHWAHPGGPAWGRYWWR 60
           MS+SSEIESLWVFALASKCSAL K+NIL+SLL   LIW  +SI +WAHPGGPAWGR   R
Sbjct: 1   MSLSSEIESLWVFALASKCSALPKQNILFSLLLLLLIWFLLSIFYWAHPGGPAWGRR-RR 60

Query: 61  RRRSNSTAAAIPGPRGLPLVGSMGLMADLAHHRIAAVADSLNATRLMAFSLGDTRVIVTC 120
           RR      + IPGPRGLP++GSM LM  L+H +IA++A SL ATRLMAFSL +TRVIVTC
Sbjct: 61  RRLHRHCPSTIPGPRGLPVIGSMRLMTGLSHQKIASMAKSLGATRLMAFSLANTRVIVTC 120

Query: 121 NPDVAKEILNSSLFADRPVKESAYSLMFNRAIGFAPYGLTGG------------------ 180
           +PDVAK+ILNSS+FADRP+KESAYSLMFNRAIGFAPYG+                     
Sbjct: 121 HPDVAKDILNSSVFADRPIKESAYSLMFNRAIGFAPYGIYWRTLRRIASHHLFCPKQIKS 180

Query: 181 -------------PSAASLPTTSSAPSKSSPPSPSAAKSLPK-WSQFYDLSDSANNDVQE 240
                           +S+ T+SS P + +  + S    +   + +FY+LSDS N +VQ 
Sbjct: 181 SESQRRQIASQMVKILSSIATSSSLPIRQTLKTASLNNIMGSVFGRFYNLSDS-NPEVQL 240

Query: 241 LQSLVDEGYDLLGLLNWSDHLPWLADFDSQKIRFRCSRLVPKVNHFVGRIIAEHRAKSDN 300
           LQ+LVDEGYDLLGLLNWSDHLP+LADFD Q IRFRCSRLVP+VN FV RII EHR KS+ 
Sbjct: 241 LQTLVDEGYDLLGLLNWSDHLPFLADFDPQNIRFRCSRLVPQVNRFVTRIINEHRQKSNP 300

Query: 301 QVLDFVDVLLSLQEADKLSDSDMIAVLWVRNDFSWDGHGGSFNEWILARMVLHNDIQRKV 360
           +++DFVDVLLSLQ+ D L+DSD+I+VLW    F          EWILARMV+H + Q KV
Sbjct: 301 EIMDFVDVLLSLQQNDNLADSDIISVLW-EMIFRGTDTVAVLIEWILARMVVHKEEQEKV 360

Query: 361 QEELDNVVGSTRAVAESDIPSLVYLTAVVKEVLRLHPPGPLLSWARLAITDTIIDGHHVP 420
           QEELDNVVG +RAV ESDIPSLVYLTAV+KEVLRLHPPGPLLSWARLAITDTI+DGHHVP
Sbjct: 361 QEELDNVVGKSRAVMESDIPSLVYLTAVIKEVLRLHPPGPLLSWARLAITDTIVDGHHVP 420

Query: 421 RGTTAMVNMWSIARDPQVWSDPLEFMPQRFVSDPG-DVEFSVMGSDLRLAPFGSGRRTCP 450
           RGTTAMVNMWSIARDPQ+WSDPLEF P RFVS  G DVEFS+MGSDLRLAPFGSGRRTCP
Sbjct: 421 RGTTAMVNMWSIARDPQIWSDPLEFKPDRFVSGGGADVEFSIMGSDLRLAPFGSGRRTCP 479

BLAST of Sgr011886 vs. NCBI nr
Match: XP_023006561.1 (cytochrome P450 78A3-like [Cucurbita maxima])

HSP 1 Score: 597.0 bits (1538), Expect = 1.4e-166
Identity = 322/481 (66.94%), Postives = 365/481 (75.88%), Query Frame = 0

Query: 1   MSMSSEIESLWVFALASKCSALTKENILWSLLFFFLIWVSVSILHWAHPGGPAWGRYWWR 60
           MS+S++IESLWVFALASKCS+LTK+ +L SLLF  LI   +SI HWAHPGGPAWG + + 
Sbjct: 1   MSLSTQIESLWVFALASKCSSLTKDTLLCSLLFLLLICFLLSIFHWAHPGGPAWGSHGYY 60

Query: 61  RRRSNSTAAAIPGPRGLPLVGSMGLMADLAHHRIAAVADSLNATRLMAFSLGDTRVIVTC 120
           RR    TAA IPGPRGLPL+GSM LM  LAH RIAA+A SL ATRLMAFSL  TRVIVT 
Sbjct: 61  RR----TAAVIPGPRGLPLIGSMTLMMGLAHQRIAAIAKSLGATRLMAFSLAYTRVIVTS 120

Query: 121 NPDVAKEILNSSLFADRPVKESAYSLMFNRAIGFAPYGL--------------------T 180
           +PDVAKEILNSS+FADRP+KESAYSLMFNRAIGFAPYGL                    +
Sbjct: 121 HPDVAKEILNSSVFADRPIKESAYSLMFNRAIGFAPYGLYWRTLRRIASHHLFSPKQIKS 180

Query: 181 GGPSAASLPTTSSAPSKSSPPSPSAAKSLPK-----------WSQFYDLSDSANNDVQEL 240
                  + +   A   S+  SP   +   K           + +FY L+DS NN+ ++L
Sbjct: 181 SESHRRHIASQMLAIFSSTTTSPHRVRDTLKRASLNSMMASVFGRFYTLTDS-NNEAEQL 240

Query: 241 QSLVDEGYDLLGLLNWSDHLPWLADFDSQKIRFRCSRLVPKVNHFVGRIIAEHRAKS-DN 300
           Q+LV EGYDLLGLLNWSDHLP+LADFD Q+IRFRCS+LVP+VN  VGRIIAEHR+ S  +
Sbjct: 241 QTLVAEGYDLLGLLNWSDHLPFLADFDPQRIRFRCSQLVPQVNRLVGRIIAEHRSNSTSD 300

Query: 301 QVLDFVDVLLSLQEADKLSDSDMIAVLWVRNDFSWDGHGGSFNEWILARMVLHNDIQRKV 360
           Q+ DFVDVLLSLQ++D LSDSD+IAVLW    F          EWILARMV+H ++Q KV
Sbjct: 301 QITDFVDVLLSLQQSDNLSDSDIIAVLW-EMIFRGTDTTAVLMEWILARMVVHTEVQEKV 360

Query: 361 QEELDNVVGSTRAVAESDIPSLVYLTAVVKEVLRLHPPGPLLSWARLAITDTIIDGHHVP 420
           Q ELD VVG +RAV ESDIPSLVYLTAVVKEVLRLHPPGPLLSWARLAITDT IDGHHVP
Sbjct: 361 QSELDTVVGRSRAVVESDIPSLVYLTAVVKEVLRLHPPGPLLSWARLAITDTTIDGHHVP 420

Query: 421 RGTTAMVNMWSIARDPQVWSDPLEFMPQRFVSDPGDVEFSVMGSDLRLAPFGSGRRTCPG 450
           +GTTAMVNMWSIARDPQVWSDPLEF P+RFVS   DVEFSVMGSDLRLAPFGSGRRTCPG
Sbjct: 421 KGTTAMVNMWSIARDPQVWSDPLEFKPERFVSGGADVEFSVMGSDLRLAPFGSGRRTCPG 475

BLAST of Sgr011886 vs. NCBI nr
Match: XP_022999908.1 (cytochrome P450 78A3-like [Cucurbita maxima])

HSP 1 Score: 592.0 bits (1525), Expect = 4.6e-165
Identity = 313/490 (63.88%), Postives = 365/490 (74.49%), Query Frame = 0

Query: 1   MSMSSEIESLWVFALASKCSALTKENILWSLLFFFLIWVSVSILHWAHPGGPAWGRYWWR 60
           MS+S++IESLWVFALASKCS LT+ NIL+S LF  LIW  VSI HWAHPGGPAWGR+ W 
Sbjct: 1   MSLSTDIESLWVFALASKCSPLTQPNILYSFLFLCLIWFLVSIFHWAHPGGPAWGRHPWC 60

Query: 61  RRRS--NSTAAAIPGPRGLPLVGSMGLMADLAHHRIAAVADSLNATRLMAFSLGDTRVIV 120
           RR S  ++T   IPGPRGLP++GSM LM  LAHHRIAA+A+SL ATRLMAFSL +TRVIV
Sbjct: 61  RRNSPLSTTTTTIPGPRGLPVIGSMTLMTGLAHHRIAAMAESLGATRLMAFSLANTRVIV 120

Query: 121 TCNPDVAKEILNSSLFADRPVKESAYSLMFNRAIGFAPYGL------------------- 180
           TCNPDVAKEILNSS+FADRP+KESAYSLMFNRA+GFAPYG+                   
Sbjct: 121 TCNPDVAKEILNSSVFADRPIKESAYSLMFNRAVGFAPYGVYWRTLRRIASQHLFCPKQI 180

Query: 181 ---------TGGPSAASLPTTSSAPSKSSPPSPSAAKSLPK----------WSQFYDLSD 240
                          A     ++  + ++        +L +          + Q Y+LSD
Sbjct: 181 KSSEFQRRQIASQMVAIFAANNNNNNNTTSSHHQVRDTLKRASLNHMMGSVFGQVYNLSD 240

Query: 241 SANNDVQELQSLVDEGYDLLGLLNWSDHLPWLADFDSQKIRFRCSRLVPKVNHFVGRIIA 300
           S NN +Q+L+SLVDEGYDLLGLLNWSDHLP+LAD D Q+IRFRCSRLVPKVN F+ RIIA
Sbjct: 241 S-NNQIQQLKSLVDEGYDLLGLLNWSDHLPFLADLDPQRIRFRCSRLVPKVNRFLNRIIA 300

Query: 301 EHRAKSDNQVLDFVDVLLSLQEADKLSDSDMIAVLWVRNDFSWDGHGGSFNEWILARMVL 360
           +HR++S  Q++DFVDVLLSLQ+ + LSDSD+IAVLW    F          EW LARMV+
Sbjct: 301 DHRSRSTQQIMDFVDVLLSLQQTENLSDSDIIAVLW-EMIFRGTDTVAVLIEWTLARMVV 360

Query: 361 HNDIQRKVQEELDNVVGSTRAVAESDIPSLVYLTAVVKEVLRLHPPGPLLSWARLAITDT 420
           H + Q KVQEELD VVG +RAV ESDIPSL YLTAVVKEVLRLHPPGPLLSWARLA +DT
Sbjct: 361 HKEEQEKVQEELDKVVGRSRAVVESDIPSLEYLTAVVKEVLRLHPPGPLLSWARLARSDT 420

Query: 421 IIDGHHVPRGTTAMVNMWSIARDPQVWSDPLEFMPQRFVSDPG-DVEFSVMGSDLRLAPF 450
           ++DGHHVPRGTTAMVNMWSIARDP+VW  P EF+PQRFV +    VEFS+MGSDLRLAPF
Sbjct: 421 MVDGHHVPRGTTAMVNMWSIARDPEVWWSPQEFIPQRFVGNKWCGVEFSIMGSDLRLAPF 480

BLAST of Sgr011886 vs. NCBI nr
Match: XP_022959466.1 (cytochrome P450 78A3 [Cucurbita moschata])

HSP 1 Score: 591.7 bits (1524), Expect = 6.0e-165
Identity = 323/482 (67.01%), Postives = 363/482 (75.31%), Query Frame = 0

Query: 1   MSMSSEIESLWVFALASKCSALTKENILWSLLFFFLIWVSVSILHWAHPGGPAWGRYWWR 60
           MS+S++I+SLWVFALASKCS+LTKE +L SLLF  LI   VSI HWAHPGGPAWG +   
Sbjct: 1   MSLSTQIDSLWVFALASKCSSLTKETLLCSLLFLLLICFLVSIFHWAHPGGPAWGSHGCY 60

Query: 61  RRRSNSTAAAIPGPRGLPLVGSMGLMADLAHHRIAAVADSLNATRLMAFSLGDTRVIVTC 120
           RR    TAA IPGPRGLPL+GSM LM  LAH RIAA+A SL ATRLMAFSL  TRVIVT 
Sbjct: 61  RR----TAAVIPGPRGLPLIGSMTLMMGLAHQRIAAIAKSLGATRLMAFSLAYTRVIVTS 120

Query: 121 NPDVAKEILNSSLFADRPVKESAYSLMFNRAIGFAPYGL--------------------- 180
           +PDVAKEILNSS+FADRP+KESAYSLMFNRAIGFAPYGL                     
Sbjct: 121 HPDVAKEILNSSVFADRPIKESAYSLMFNRAIGFAPYGLYWRTLRRIASHHLFSPKQIKS 180

Query: 181 ---------TGGPSAASLPTTSSAPSKSSPPSPSAAKSL--PKWSQFYDLSDSANNDVQE 240
                    +   +  S  TTSS          ++  S+    + +FY L+DS NN+ ++
Sbjct: 181 SESHRRHIASQMLTIFSSTTTSSTHRVRDTLKRASLNSMMASVFGRFYTLTDS-NNEAEQ 240

Query: 241 LQSLVDEGYDLLGLLNWSDHLPWLADFDSQKIRFRCSRLVPKVNHFVGRIIAEHRAKS-D 300
           LQ+LV EGYDLLGLLNWSDHLP+LADFD Q+IRFRCS+LVP+VN  VGRIIAEHR+ S  
Sbjct: 241 LQTLVAEGYDLLGLLNWSDHLPFLADFDPQRIRFRCSQLVPQVNQLVGRIIAEHRSNSTP 300

Query: 301 NQVLDFVDVLLSLQEADKLSDSDMIAVLWVRNDFSWDGHGGSFNEWILARMVLHNDIQRK 360
            Q+ DFVDVLLSLQ++D LSDSD+IAVLW    F          EWILARMV+H ++Q K
Sbjct: 301 QQITDFVDVLLSLQQSDNLSDSDIIAVLW-EMIFRGTDTTAVLMEWILARMVVHPEVQEK 360

Query: 361 VQEELDNVVGSTRAVAESDIPSLVYLTAVVKEVLRLHPPGPLLSWARLAITDTIIDGHHV 420
           VQ ELD VVG +R V ESDIPSLVYLTAVVKEVLRLHPPGPLLSWARLAITDT IDGHHV
Sbjct: 361 VQSELDTVVGRSRGVVESDIPSLVYLTAVVKEVLRLHPPGPLLSWARLAITDTTIDGHHV 420

Query: 421 PRGTTAMVNMWSIARDPQVWSDPLEFMPQRFVSDPGDVEFSVMGSDLRLAPFGSGRRTCP 450
           P+GTTAMVNMWSIARDPQVWSDPLEF P+RFVS   DVEFSVMGSDLRLAPFGSGRRTCP
Sbjct: 421 PKGTTAMVNMWSIARDPQVWSDPLEFKPERFVSGGADVEFSVMGSDLRLAPFGSGRRTCP 476

BLAST of Sgr011886 vs. NCBI nr
Match: KAG6575238.1 (Cytochrome P450 78A3, partial [Cucurbita argyrosperma subsp. sororia] >KAG7013805.1 Cytochrome P450 78A3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 590.5 bits (1521), Expect = 1.3e-164
Identity = 322/482 (66.80%), Postives = 363/482 (75.31%), Query Frame = 0

Query: 1   MSMSSEIESLWVFALASKCSALTKENILWSLLFFFLIWVSVSILHWAHPGGPAWGRYWWR 60
           MS+S++I+SLWVFALASKCS+LTK+ +L SLLF  LI   VSI HWAHPGGPAWG +   
Sbjct: 1   MSLSTQIDSLWVFALASKCSSLTKDTLLCSLLFLLLICFLVSIFHWAHPGGPAWGSHGCY 60

Query: 61  RRRSNSTAAAIPGPRGLPLVGSMGLMADLAHHRIAAVADSLNATRLMAFSLGDTRVIVTC 120
           RR    TAA IPGPRGLPL+GSM LM  LAH RIAA+A SL ATRLMAFSL  TRVIVT 
Sbjct: 61  RR----TAAVIPGPRGLPLIGSMTLMMGLAHQRIAAIAKSLGATRLMAFSLAYTRVIVTS 120

Query: 121 NPDVAKEILNSSLFADRPVKESAYSLMFNRAIGFAPYGL--------------------- 180
           +PDVAKEILNSS+FADRP+KESAYSLMFNRAIGFAPYGL                     
Sbjct: 121 HPDVAKEILNSSVFADRPIKESAYSLMFNRAIGFAPYGLYWRTLRRIASHHLFSPKQIKS 180

Query: 181 ---------TGGPSAASLPTTSSAPSKSSPPSPSAAKSL--PKWSQFYDLSDSANNDVQE 240
                    +   +  S  TTSS          ++  S+    + +FY L+DS NN+ ++
Sbjct: 181 SESHRRHIASQMLTIFSSTTTSSTHRVRDTLKRASLNSMMASVFGRFYTLTDS-NNEAEQ 240

Query: 241 LQSLVDEGYDLLGLLNWSDHLPWLADFDSQKIRFRCSRLVPKVNHFVGRIIAEHRAKS-D 300
           LQ+LV EGYDLLGLLNWSDHLP+LADFD Q+IRFRCS+LVP+VN  VGRIIAEHR+ S  
Sbjct: 241 LQTLVAEGYDLLGLLNWSDHLPFLADFDPQRIRFRCSQLVPQVNQLVGRIIAEHRSNSTP 300

Query: 301 NQVLDFVDVLLSLQEADKLSDSDMIAVLWVRNDFSWDGHGGSFNEWILARMVLHNDIQRK 360
            Q+ DFVDVLLSLQ++D LSDSD+IAVLW    F          EWILARMV+H ++Q K
Sbjct: 301 QQITDFVDVLLSLQQSDNLSDSDIIAVLW-EMIFRGTDTTAVLMEWILARMVVHPEVQEK 360

Query: 361 VQEELDNVVGSTRAVAESDIPSLVYLTAVVKEVLRLHPPGPLLSWARLAITDTIIDGHHV 420
           VQ ELD VVG +R V ESDIPSLVYLTAVVKEVLRLHPPGPLLSWARLAITDT IDGHHV
Sbjct: 361 VQSELDTVVGRSRGVVESDIPSLVYLTAVVKEVLRLHPPGPLLSWARLAITDTTIDGHHV 420

Query: 421 PRGTTAMVNMWSIARDPQVWSDPLEFMPQRFVSDPGDVEFSVMGSDLRLAPFGSGRRTCP 450
           P+GTTAMVNMWSIARDPQVWSDPLEF P+RFVS   DVEFSVMGSDLRLAPFGSGRRTCP
Sbjct: 421 PKGTTAMVNMWSIARDPQVWSDPLEFKPERFVSGGADVEFSVMGSDLRLAPFGSGRRTCP 476

BLAST of Sgr011886 vs. ExPASy Swiss-Prot
Match: O48927 (Cytochrome P450 78A3 OS=Glycine max OX=3847 GN=CYP78A3 PE=2 SV=1)

HSP 1 Score: 515.0 bits (1325), Expect = 9.4e-145
Identity = 280/479 (58.46%), Postives = 328/479 (68.48%), Query Frame = 0

Query: 3   MSSEI-ESLWVFALASKCSALTKENILWSLLFFFLIWVSVSILHWAHPGGPAWGRYWWRR 62
           M+S I ++LW+ AL SKC   T+EN+ W LL    +W++++  +W+HPGGPAWG+Y+   
Sbjct: 1   MTSHIDDNLWIIALTSKC---TQENLAWVLLIMGSLWLTMTFYYWSHPGGPAWGKYY--- 60

Query: 63  RRSNSTAAAIPGPRGLPLVGSMGLMADLAHHRIAAVADSLNATRLMAFSLGDTRVIVTCN 122
              +   + IPGP+G PL+GSMGLM  LAHHRIAA A +  A RLMAFSLGDTRVIVTC+
Sbjct: 61  -TYSPPLSIIPGPKGFPLIGSMGLMTSLAHHRIAAAAATCRAKRLMAFSLGDTRVIVTCH 120

Query: 123 PDVAKEILNSSLFADRPVKESAYSLMFNRAIGFAPYGLTGGP---------------SAA 182
           PDVAKEILNSS+FADRPVKESAYSLMFNRAIGFA YG+                    A+
Sbjct: 121 PDVAKEILNSSVFADRPVKESAYSLMFNRAIGFASYGVYWRSLRRIASNHLFCPRQIKAS 180

Query: 183 SLPTTSSAPSKSSPPSPSAAKSL----------------PKWSQFYDLSDSANNDVQELQ 242
            L  +  A       +    +SL                  + Q Y L D  N+ +++L 
Sbjct: 181 ELQRSQIAAQMVHILNNKRHRSLRVRQVLKKASLSNMMCSVFGQEYKLHD-PNSGMEDLG 240

Query: 243 SLVDEGYDLLGLLNWSDHLPWLADFDSQKIRFRCSRLVPKVNHFVGRIIAEHRAKSDNQV 302
            LVD+GYDLLGL NW+DHLP+LA FD+Q IRFRCS LVP VN FVG IIAEHRA      
Sbjct: 241 ILVDQGYDLLGLFNWADHLPFLAHFDAQNIRFRCSNLVPMVNRFVGTIIAEHRASKTETN 300

Query: 303 LDFVDVLLSLQEADKLSDSDMIAVLWVRNDFSWDGHGGSFNEWILARMVLHNDIQRKVQE 362
            DFVDVLLSL E D+LSDSDMIAVLW    F          EWILARM LH  +Q KVQE
Sbjct: 301 RDFVDVLLSLPEPDQLSDSDMIAVLW-EMIFRGTDTVAVLIEWILARMALHPHVQSKVQE 360

Query: 363 ELDNVVGSTRAVAESDIPSLVYLTAVVKEVLRLHPPGPLLSWARLAITDTIIDGHHVPRG 422
           ELD VVG  RAVAE D+  + YL AVVKEVLRLHPPGPLLSWARL+I DT IDG+HVP G
Sbjct: 361 ELDAVVGKARAVAEDDVAVMTYLPAVVKEVLRLHPPGPLLSWARLSINDTTIDGYHVPAG 420

Query: 423 TTAMVNMWSIARDPQVWSDPLEFMPQRFVSDPGDVEFSVMGSDLRLAPFGSGRRTCPGR 450
           TTAMVN W+I RDP VW DPLEFMP+RFV+  GD EFS++GSD RLAPFGSGRR CPG+
Sbjct: 421 TTAMVNTWAICRDPHVWKDPLEFMPERFVTAGGDAEFSILGSDPRLAPFGSGRRACPGK 470

BLAST of Sgr011886 vs. ExPASy Swiss-Prot
Match: Q9SLP1 (Cytochrome P450 78A9 OS=Arabidopsis thaliana OX=3702 GN=CYP78A9 PE=1 SV=1)

HSP 1 Score: 511.1 bits (1315), Expect = 1.4e-143
Identity = 282/471 (59.87%), Postives = 327/471 (69.43%), Query Frame = 0

Query: 12  VFALASKCSALTKENILWSLLFFFLIWVSVSILHWAHPGGPAWGRYWWRRRRSNSTAAAI 71
           + AL SKCS LT+ N+  SLL   L  +++S+  W+HPGGPAWG+Y+  RRR  +    I
Sbjct: 11  LLALLSKCSLLTQTNLALSLLVASLASLALSLFFWSHPGGPAWGKYFLHRRRQTT---VI 70

Query: 72  PGPRGLPLVGSMGLMAD-LAHHRIAAVADSLNATRLMAFSLGDTRVIVTCNPDVAKEILN 131
           PGPRGLP VGSM LM++ LAH  IAA A+   A RLMAFSLG+TRVIVTCNPDVAKEILN
Sbjct: 71  PGPRGLPFVGSMSLMSNTLAHRCIAATAEKFRAERLMAFSLGETRVIVTCNPDVAKEILN 130

Query: 132 SSLFADRPVKESAYSLMFNRAIGFAPYGL-------TGGPSAASLPTTSSAPSKSSPPSP 191
           S +FADRPVKESAYSLMFNRAIGFAPYG+              S      + ++ S  + 
Sbjct: 131 SPVFADRPVKESAYSLMFNRAIGFAPYGVYWRTLRKIASNHLFSPKQIKRSETQRSVIAN 190

Query: 192 SAAKSLPKWSQFYDL--------SDSANN-----------------DVQELQSLVDEGYD 251
              K L K S    L        + S NN                 +V EL+ LV+EGYD
Sbjct: 191 QIVKCLTKQSNTKGLCFARDLIKTASLNNMMCSVFGKEYELEEEHEEVSELRELVEEGYD 250

Query: 252 LLGLLNWSDHLPWLADFDSQKIRFRCSRLVPKVNHFVGRIIAEHRAKSDNQVLDFVDVLL 311
           LLG LNW+DHLPWL++FD Q+IR RCS LVPKVN FV RII++HR ++ +   DFVDVLL
Sbjct: 251 LLGTLNWTDHLPWLSEFDPQRIRSRCSNLVPKVNRFVNRIISDHREQTRDSPSDFVDVLL 310

Query: 312 SLQEADKLSDSDMIAVLWVRNDFSWDGHGGSFNEWILARMVLHNDIQRKVQEELDNVVGS 371
           SL   DKLSD D+IAVLW    F          EWILARMVLH DIQ  V  ELD +VG 
Sbjct: 311 SLDGPDKLSDPDIIAVLW-EMIFRGTDTVAVLIEWILARMVLHPDIQSTVHNELDQIVGR 370

Query: 372 TRAVAESDIPSLVYLTAVVKEVLRLHPPGPLLSWARLAITDTIIDGHHVPRGTTAMVNMW 431
           +RAV ESD+ SLVYLTAVVKEVLRLHPPGPLLSWARLAITDTIIDG  VP GTTAMVNMW
Sbjct: 371 SRAVEESDVVSLVYLTAVVKEVLRLHPPGPLLSWARLAITDTIIDGRRVPAGTTAMVNMW 430

Query: 432 SIARDPQVWSDPLEFMPQRFVSDPGDVEFSVMGSDLRLAPFGSGRRTCPGR 450
           +IA DP VW +PLEF P+RFV+  G+VEFSV+GSDLRLAPFGSGRR CPG+
Sbjct: 431 AIAHDPHVWENPLEFKPERFVAKEGEVEFSVLGSDLRLAPFGSGRRVCPGK 477

BLAST of Sgr011886 vs. ExPASy Swiss-Prot
Match: Q9ZNR0 (Cytochrome P450 78A6 OS=Arabidopsis thaliana OX=3702 GN=CYP78A6 PE=2 SV=1)

HSP 1 Score: 510.0 bits (1312), Expect = 3.0e-143
Identity = 278/478 (58.16%), Postives = 333/478 (69.67%), Query Frame = 0

Query: 3   MSSEIESLWVFALASKCSALTKENILWSLLFFFLIWVSVSILHWAHPGGPAWGRYWWRRR 62
           M++++ES  +FAL SKCS L++ N+ +SLL   +IW+++S+  W +PGGPAWG+Y + R 
Sbjct: 1   MATKLESSLIFALLSKCSVLSQTNLAFSLLAVTIIWLAISLFLWTYPGGPAWGKYLFGRL 60

Query: 63  RSNS--TAAAIPGPRGLPLVGSMGLMAD-LAHHRIAAVADSLNATRLMAFSLGDTRVIVT 122
            S S  T   IPGP+G PLVGSM LM+  LAH RIA  A+   A RLMAFSLG+TRVIVT
Sbjct: 61  ISGSYKTGNVIPGPKGFPLVGSMSLMSSTLAHRRIADAAEKFGAKRLMAFSLGETRVIVT 120

Query: 123 CNPDVAKEILNSSLFADRPVKESAYSLMFNRAIGFAPYG-------------LTGGPSAA 182
           CNPDVAKEILNS +FADRPVKESAYSLMFNRAIGFAP+G             L       
Sbjct: 121 CNPDVAKEILNSPVFADRPVKESAYSLMFNRAIGFAPHGVYWRTLRRIASNHLFSTKQIR 180

Query: 183 SLPTTSSAPS--------KSSPPSPSAAKSLPKWSQFYDLSDSA-------NNDVQELQS 242
              T     S        K S   P   + L K +   ++  S          +  EL+ 
Sbjct: 181 RAETQRRVISSQMVEFLEKQSSNEPCFVRELLKTASLNNMMCSVFGQEYELEKNHVELRE 240

Query: 243 LVDEGYDLLGLLNWSDHLPWLADFDSQKIRFRCSRLVPKVNHFVGRIIAEHRAKSDNQVL 302
           +V+EGYDLLG LNW+DHLPWL++FD Q++R RCS LVPKVN FV RII+EHR ++ +   
Sbjct: 241 MVEEGYDLLGTLNWTDHLPWLSEFDPQRLRSRCSTLVPKVNRFVSRIISEHRNQTGDLPR 300

Query: 303 DFVDVLLSLQEADKLSDSDMIAVLWVRNDFSWDGHGGSFNEWILARMVLHNDIQRKVQEE 362
           DFVDVLLSL  +DKLSD D+IAVLW    F          EWILARMVLH D+Q  VQ E
Sbjct: 301 DFVDVLLSLHGSDKLSDPDIIAVLW-EMIFRGTDTVAVLIEWILARMVLHPDMQSTVQNE 360

Query: 363 LDNVVGSTRAVAESDIPSLVYLTAVVKEVLRLHPPGPLLSWARLAITDTIIDGHHVPRGT 422
           LD VVG +RA+ ESD+ SL YLTAVVKEVLRLHPPGPLLSWARLAITDTI+DG  VP GT
Sbjct: 361 LDQVVGKSRALDESDLASLPYLTAVVKEVLRLHPPGPLLSWARLAITDTIVDGRLVPAGT 420

Query: 423 TAMVNMWSIARDPQVWSDPLEFMPQRFVSDPGDVEFSVMGSDLRLAPFGSGRRTCPGR 450
           TAMVNMW+++ DP VW DPLEF P+RFV+  G+VEFSV+GSDLRLAPFGSGRR CPG+
Sbjct: 421 TAMVNMWAVSHDPHVWVDPLEFKPERFVAKEGEVEFSVLGSDLRLAPFGSGRRICPGK 477

BLAST of Sgr011886 vs. ExPASy Swiss-Prot
Match: O65012 (Cytochrome P450 78A4 OS=Pinus radiata OX=3347 GN=CYP78A4 PE=2 SV=1)

HSP 1 Score: 345.1 bits (884), Expect = 1.3e-93
Identity = 207/461 (44.90%), Postives = 268/461 (58.13%), Query Frame = 0

Query: 29  WSLLFFFLIWVSVSIL-HWAHPGGPAWGRYWWRRRRSNSTAAAIPGPRGLPLVGS-MGLM 88
           W L    LI    ++L  W  PGG AW     R +R      AIPGP+G P++GS M + 
Sbjct: 42  WPLYIALLIVAVCAVLVSWLSPGGCAWA---GRHKRGR---VAIPGPKGWPIIGSLMDMS 101

Query: 89  ADLAHHRIAAVADSLNATRLMAFSLGDTRVIVTCNPDVAKEILNSSLFADRPVKESAYSL 148
             L H ++ ++A    A +LM+FSLG T  ++T +P+VA+E+L S  FA+RP+K+SA  L
Sbjct: 102 VGLPHRKLESLARLHGAKQLMSFSLGCTPAVITSDPEVARELLTSPHFANRPLKQSAQQL 161

Query: 149 MFNRAIGFAPYG----LTGGPSAASL--PTTSSAPSKSSPPSPSA----------AKSLP 208
           +F RAIGFAP G    L    ++A L  P   +A          A          +K + 
Sbjct: 162 LFGRAIGFAPNGGYWRLLRRIASAHLFAPRRIAAHEAGRQADVVAMLDDIQKEYHSKGVV 221

Query: 209 K-----------------WSQFYDLSDSANNDVQELQSLVDEGYDLLGLLNWSDHLPWLA 268
           +                 + + +D+S   N +V++L+ +VDEG+ LLG  NW+DHLPWL 
Sbjct: 222 RVRRHLQGAALNNIMGSVFGRRFDMSHE-NEEVKKLREMVDEGFQLLGAFNWADHLPWLR 281

Query: 269 DFDSQKIRFRCSRLVPKVNHFVGRIIAEHR----AKSDNQVLDFVDVLLSLQEADKLSDS 328
             D  +I  RC+RLVP+V  FV  II +HR     +      DFVDVLLSLQ  DKL + 
Sbjct: 282 PLDPLRIHARCARLVPRVTTFVSNIIEQHRREEQRRESGDQCDFVDVLLSLQGEDKLDEE 341

Query: 329 DMIAVLWVRNDFSWDGHGGSFNEWILARMVLHNDIQRKVQEELDNVVGSTRAVAESDIPS 388
           DMIAVLW    F          EW +A +VLH + Q+K Q ELD VVG  R+V +SDIP 
Sbjct: 342 DMIAVLW-EMIFRGTDTTALLTEWTMAELVLHPEAQKKAQAELDAVVGHDRSVKDSDIPK 401

Query: 389 LVYLTAVVKEVLRLHPPGPLLSWARLAITDTII-DGHHVPRGTTAMVNMWSIARDPQVWS 448
           L Y+ AVVKE LR+HPPGPLLSWARL+  D  + DG  VP GTTAMVNMWSI  DP +W 
Sbjct: 402 LPYIQAVVKEALRMHPPGPLLSWARLSTEDVNMGDGMCVPAGTTAMVNMWSITHDPNIWE 461

Query: 449 DPLEFMPQRFVSDPGDVEFSVMGSDLRLAPFGSGRRTCPGR 450
            P EF P+RFV   G  E  V G+DLRLAPFG+GRR CPG+
Sbjct: 462 SPYEFRPERFVVFEGGEEVDVRGNDLRLAPFGAGRRVCPGK 494

BLAST of Sgr011886 vs. ExPASy Swiss-Prot
Match: Q9FIB0 (Cytochrome P450 78A7 OS=Arabidopsis thaliana OX=3702 GN=CYP78A7 PE=2 SV=1)

HSP 1 Score: 335.9 bits (860), Expect = 7.8e-91
Identity = 209/489 (42.74%), Postives = 286/489 (58.49%), Query Frame = 0

Query: 1   MSMSSEIESLWVFALASKCSALTKENILWSLLFFFLIWVSVSILHWAHPGGPAWGRYWWR 60
           M+++S+  S W+ AL +   +    ++  +L + FL  V +SI+ WA  GG   G   W+
Sbjct: 4   MNLASKETSYWMIALPAGFGSQNLHDVS-TLGYLFLAVVFLSIVTWALAGG---GGVAWK 63

Query: 61  RRRSNSTAAAIPGPRGLPLVGSM-GLMADLAHHRIAAVADSLNATRLMAFSLGDTRVIVT 120
             R+     AIPGPRG+P+ GS+  L   LAH  +AA+A S   T +MAFSLG T VIV 
Sbjct: 64  NGRNRLGRVAIPGPRGIPVFGSLFTLSRGLAHRTLAAMAWSRANTEIMAFSLGSTPVIVA 123

Query: 121 CNPDVAKEILNSSLFADRPVKESAYSLMFNRAIGFAPYG-------------------LT 180
             P++A+EIL S  FADRPVK+SA SLMF+RAIGFAP G                   + 
Sbjct: 124 SEPNIAREILMSPHFADRPVKQSAKSLMFSRAIGFAPNGTYWRMLRRIASTHLFAPRRIL 183

Query: 181 GGPSAASLPTTSSAPSKSSPPSPSAAKSLPKWSQFYDLSD-------------SANNDVQ 240
              +   L       + S   + + +  L K  Q   L++             +   D+ 
Sbjct: 184 AHEAGRQLDCAEMVKAVSVEQNGAGSVVLRKHLQLAALNNIMGSVFGRRYDPLAQKEDLD 243

Query: 241 ELQSLVDEGYDLLGLLNWSDHLPWLADF-DSQKIRFRCSRLVPKVNHFVGRIIAEHRAKS 300
           EL S+V EG++LLG  NWSD+LPWL  F DS ++  RCS LVP++   V +II EHR  +
Sbjct: 244 ELTSMVREGFELLGAFNWSDYLPWLGYFYDSIRLNQRCSDLVPRIRTLVKKIIDEHRVSN 303

Query: 301 DNQ---VLDFVDVLLSLQEADKLSDSDMIAVLWVRNDFSWDGHGGSFNEWILARMVLHND 360
             +   + DFVDVLLSL   +KL + DMIAVLW    F          EW +A +VL+ +
Sbjct: 304 SEKKRDIGDFVDVLLSLDGDEKLQEDDMIAVLW-EMIFRGTDTTALLTEWTMAELVLNPN 363

Query: 361 IQRKVQEELDNVV--GSTRAVAESDIPSLVYLTAVVKEVLRLHPPGPLLSWARLAITDT- 420
           +Q K+++E+   V  G+   VA++D+  L YL AVVKE LRLHPPGPLLSWARL+ +D  
Sbjct: 364 VQTKLRDEILTAVGDGADGDVADADLAKLPYLNAVVKETLRLHPPGPLLSWARLSTSDVQ 423

Query: 421 IIDGHHVPRGTTAMVNMWSIARDPQVWSDPLEFMPQRFVSDPGDVEFSVMGSDLRLAPFG 450
           + +G  +P+GTTAMVNMW+I  D  VWSDPL+F P+RF    G+ +  + G DLRLAPFG
Sbjct: 424 LSNGMVIPKGTTAMVNMWAITHDQTVWSDPLKFDPERFT---GNADMDIRGGDLRLAPFG 483

BLAST of Sgr011886 vs. ExPASy TrEMBL
Match: A0A6J1KY33 (cytochrome P450 78A3-like OS=Cucurbita maxima OX=3661 GN=LOC111499249 PE=3 SV=1)

HSP 1 Score: 597.0 bits (1538), Expect = 7.0e-167
Identity = 322/481 (66.94%), Postives = 365/481 (75.88%), Query Frame = 0

Query: 1   MSMSSEIESLWVFALASKCSALTKENILWSLLFFFLIWVSVSILHWAHPGGPAWGRYWWR 60
           MS+S++IESLWVFALASKCS+LTK+ +L SLLF  LI   +SI HWAHPGGPAWG + + 
Sbjct: 1   MSLSTQIESLWVFALASKCSSLTKDTLLCSLLFLLLICFLLSIFHWAHPGGPAWGSHGYY 60

Query: 61  RRRSNSTAAAIPGPRGLPLVGSMGLMADLAHHRIAAVADSLNATRLMAFSLGDTRVIVTC 120
           RR    TAA IPGPRGLPL+GSM LM  LAH RIAA+A SL ATRLMAFSL  TRVIVT 
Sbjct: 61  RR----TAAVIPGPRGLPLIGSMTLMMGLAHQRIAAIAKSLGATRLMAFSLAYTRVIVTS 120

Query: 121 NPDVAKEILNSSLFADRPVKESAYSLMFNRAIGFAPYGL--------------------T 180
           +PDVAKEILNSS+FADRP+KESAYSLMFNRAIGFAPYGL                    +
Sbjct: 121 HPDVAKEILNSSVFADRPIKESAYSLMFNRAIGFAPYGLYWRTLRRIASHHLFSPKQIKS 180

Query: 181 GGPSAASLPTTSSAPSKSSPPSPSAAKSLPK-----------WSQFYDLSDSANNDVQEL 240
                  + +   A   S+  SP   +   K           + +FY L+DS NN+ ++L
Sbjct: 181 SESHRRHIASQMLAIFSSTTTSPHRVRDTLKRASLNSMMASVFGRFYTLTDS-NNEAEQL 240

Query: 241 QSLVDEGYDLLGLLNWSDHLPWLADFDSQKIRFRCSRLVPKVNHFVGRIIAEHRAKS-DN 300
           Q+LV EGYDLLGLLNWSDHLP+LADFD Q+IRFRCS+LVP+VN  VGRIIAEHR+ S  +
Sbjct: 241 QTLVAEGYDLLGLLNWSDHLPFLADFDPQRIRFRCSQLVPQVNRLVGRIIAEHRSNSTSD 300

Query: 301 QVLDFVDVLLSLQEADKLSDSDMIAVLWVRNDFSWDGHGGSFNEWILARMVLHNDIQRKV 360
           Q+ DFVDVLLSLQ++D LSDSD+IAVLW    F          EWILARMV+H ++Q KV
Sbjct: 301 QITDFVDVLLSLQQSDNLSDSDIIAVLW-EMIFRGTDTTAVLMEWILARMVVHTEVQEKV 360

Query: 361 QEELDNVVGSTRAVAESDIPSLVYLTAVVKEVLRLHPPGPLLSWARLAITDTIIDGHHVP 420
           Q ELD VVG +RAV ESDIPSLVYLTAVVKEVLRLHPPGPLLSWARLAITDT IDGHHVP
Sbjct: 361 QSELDTVVGRSRAVVESDIPSLVYLTAVVKEVLRLHPPGPLLSWARLAITDTTIDGHHVP 420

Query: 421 RGTTAMVNMWSIARDPQVWSDPLEFMPQRFVSDPGDVEFSVMGSDLRLAPFGSGRRTCPG 450
           +GTTAMVNMWSIARDPQVWSDPLEF P+RFVS   DVEFSVMGSDLRLAPFGSGRRTCPG
Sbjct: 421 KGTTAMVNMWSIARDPQVWSDPLEFKPERFVSGGADVEFSVMGSDLRLAPFGSGRRTCPG 475

BLAST of Sgr011886 vs. ExPASy TrEMBL
Match: A0A6J1KL15 (cytochrome P450 78A3-like OS=Cucurbita maxima OX=3661 GN=LOC111494245 PE=3 SV=1)

HSP 1 Score: 592.0 bits (1525), Expect = 2.2e-165
Identity = 313/490 (63.88%), Postives = 365/490 (74.49%), Query Frame = 0

Query: 1   MSMSSEIESLWVFALASKCSALTKENILWSLLFFFLIWVSVSILHWAHPGGPAWGRYWWR 60
           MS+S++IESLWVFALASKCS LT+ NIL+S LF  LIW  VSI HWAHPGGPAWGR+ W 
Sbjct: 1   MSLSTDIESLWVFALASKCSPLTQPNILYSFLFLCLIWFLVSIFHWAHPGGPAWGRHPWC 60

Query: 61  RRRS--NSTAAAIPGPRGLPLVGSMGLMADLAHHRIAAVADSLNATRLMAFSLGDTRVIV 120
           RR S  ++T   IPGPRGLP++GSM LM  LAHHRIAA+A+SL ATRLMAFSL +TRVIV
Sbjct: 61  RRNSPLSTTTTTIPGPRGLPVIGSMTLMTGLAHHRIAAMAESLGATRLMAFSLANTRVIV 120

Query: 121 TCNPDVAKEILNSSLFADRPVKESAYSLMFNRAIGFAPYGL------------------- 180
           TCNPDVAKEILNSS+FADRP+KESAYSLMFNRA+GFAPYG+                   
Sbjct: 121 TCNPDVAKEILNSSVFADRPIKESAYSLMFNRAVGFAPYGVYWRTLRRIASQHLFCPKQI 180

Query: 181 ---------TGGPSAASLPTTSSAPSKSSPPSPSAAKSLPK----------WSQFYDLSD 240
                          A     ++  + ++        +L +          + Q Y+LSD
Sbjct: 181 KSSEFQRRQIASQMVAIFAANNNNNNNTTSSHHQVRDTLKRASLNHMMGSVFGQVYNLSD 240

Query: 241 SANNDVQELQSLVDEGYDLLGLLNWSDHLPWLADFDSQKIRFRCSRLVPKVNHFVGRIIA 300
           S NN +Q+L+SLVDEGYDLLGLLNWSDHLP+LAD D Q+IRFRCSRLVPKVN F+ RIIA
Sbjct: 241 S-NNQIQQLKSLVDEGYDLLGLLNWSDHLPFLADLDPQRIRFRCSRLVPKVNRFLNRIIA 300

Query: 301 EHRAKSDNQVLDFVDVLLSLQEADKLSDSDMIAVLWVRNDFSWDGHGGSFNEWILARMVL 360
           +HR++S  Q++DFVDVLLSLQ+ + LSDSD+IAVLW    F          EW LARMV+
Sbjct: 301 DHRSRSTQQIMDFVDVLLSLQQTENLSDSDIIAVLW-EMIFRGTDTVAVLIEWTLARMVV 360

Query: 361 HNDIQRKVQEELDNVVGSTRAVAESDIPSLVYLTAVVKEVLRLHPPGPLLSWARLAITDT 420
           H + Q KVQEELD VVG +RAV ESDIPSL YLTAVVKEVLRLHPPGPLLSWARLA +DT
Sbjct: 361 HKEEQEKVQEELDKVVGRSRAVVESDIPSLEYLTAVVKEVLRLHPPGPLLSWARLARSDT 420

Query: 421 IIDGHHVPRGTTAMVNMWSIARDPQVWSDPLEFMPQRFVSDPG-DVEFSVMGSDLRLAPF 450
           ++DGHHVPRGTTAMVNMWSIARDP+VW  P EF+PQRFV +    VEFS+MGSDLRLAPF
Sbjct: 421 MVDGHHVPRGTTAMVNMWSIARDPEVWWSPQEFIPQRFVGNKWCGVEFSIMGSDLRLAPF 480

BLAST of Sgr011886 vs. ExPASy TrEMBL
Match: A0A6J1H4X9 (cytochrome P450 78A3 OS=Cucurbita moschata OX=3662 GN=LOC111460432 PE=3 SV=1)

HSP 1 Score: 591.7 bits (1524), Expect = 2.9e-165
Identity = 323/482 (67.01%), Postives = 363/482 (75.31%), Query Frame = 0

Query: 1   MSMSSEIESLWVFALASKCSALTKENILWSLLFFFLIWVSVSILHWAHPGGPAWGRYWWR 60
           MS+S++I+SLWVFALASKCS+LTKE +L SLLF  LI   VSI HWAHPGGPAWG +   
Sbjct: 1   MSLSTQIDSLWVFALASKCSSLTKETLLCSLLFLLLICFLVSIFHWAHPGGPAWGSHGCY 60

Query: 61  RRRSNSTAAAIPGPRGLPLVGSMGLMADLAHHRIAAVADSLNATRLMAFSLGDTRVIVTC 120
           RR    TAA IPGPRGLPL+GSM LM  LAH RIAA+A SL ATRLMAFSL  TRVIVT 
Sbjct: 61  RR----TAAVIPGPRGLPLIGSMTLMMGLAHQRIAAIAKSLGATRLMAFSLAYTRVIVTS 120

Query: 121 NPDVAKEILNSSLFADRPVKESAYSLMFNRAIGFAPYGL--------------------- 180
           +PDVAKEILNSS+FADRP+KESAYSLMFNRAIGFAPYGL                     
Sbjct: 121 HPDVAKEILNSSVFADRPIKESAYSLMFNRAIGFAPYGLYWRTLRRIASHHLFSPKQIKS 180

Query: 181 ---------TGGPSAASLPTTSSAPSKSSPPSPSAAKSL--PKWSQFYDLSDSANNDVQE 240
                    +   +  S  TTSS          ++  S+    + +FY L+DS NN+ ++
Sbjct: 181 SESHRRHIASQMLTIFSSTTTSSTHRVRDTLKRASLNSMMASVFGRFYTLTDS-NNEAEQ 240

Query: 241 LQSLVDEGYDLLGLLNWSDHLPWLADFDSQKIRFRCSRLVPKVNHFVGRIIAEHRAKS-D 300
           LQ+LV EGYDLLGLLNWSDHLP+LADFD Q+IRFRCS+LVP+VN  VGRIIAEHR+ S  
Sbjct: 241 LQTLVAEGYDLLGLLNWSDHLPFLADFDPQRIRFRCSQLVPQVNQLVGRIIAEHRSNSTP 300

Query: 301 NQVLDFVDVLLSLQEADKLSDSDMIAVLWVRNDFSWDGHGGSFNEWILARMVLHNDIQRK 360
            Q+ DFVDVLLSLQ++D LSDSD+IAVLW    F          EWILARMV+H ++Q K
Sbjct: 301 QQITDFVDVLLSLQQSDNLSDSDIIAVLW-EMIFRGTDTTAVLMEWILARMVVHPEVQEK 360

Query: 361 VQEELDNVVGSTRAVAESDIPSLVYLTAVVKEVLRLHPPGPLLSWARLAITDTIIDGHHV 420
           VQ ELD VVG +R V ESDIPSLVYLTAVVKEVLRLHPPGPLLSWARLAITDT IDGHHV
Sbjct: 361 VQSELDTVVGRSRGVVESDIPSLVYLTAVVKEVLRLHPPGPLLSWARLAITDTTIDGHHV 420

Query: 421 PRGTTAMVNMWSIARDPQVWSDPLEFMPQRFVSDPGDVEFSVMGSDLRLAPFGSGRRTCP 450
           P+GTTAMVNMWSIARDPQVWSDPLEF P+RFVS   DVEFSVMGSDLRLAPFGSGRRTCP
Sbjct: 421 PKGTTAMVNMWSIARDPQVWSDPLEFKPERFVSGGADVEFSVMGSDLRLAPFGSGRRTCP 476

BLAST of Sgr011886 vs. ExPASy TrEMBL
Match: A0A2N9IN33 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS54868 PE=3 SV=1)

HSP 1 Score: 587.8 bits (1514), Expect = 4.2e-164
Identity = 306/485 (63.09%), Postives = 361/485 (74.43%), Query Frame = 0

Query: 3   MSSEIESLWVFALASKCSALTKENILWSLLFFFLIWVSVSILHWAHPGGPAWGRYWWRRR 62
           M ++IESLWVFAL SKC A T+ NI W +L   L W+ +++++WAHPGGPAWGRY WR R
Sbjct: 1   MRTDIESLWVFALTSKCQAFTQANIAWPILVMCLAWLGMTLIYWAHPGGPAWGRYGWRTR 60

Query: 63  RSNSTAA--------AIPGPRGLPLVGSMGLMADLAHHRIAAVADSLNATRLMAFSLGDT 122
           +S++T           IPGPRGLPLVGSM LM  LAHHRIAA A +  A RLMAFSLGDT
Sbjct: 61  KSSTTTTTTSLANKPTIPGPRGLPLVGSMNLMVSLAHHRIAAAAKAFQAKRLMAFSLGDT 120

Query: 123 RVIVTCNPDVAKEILNSSLFADRPVKESAYSLMFNRAIGFAPYGLTGGPSAASLPTTSSA 182
           RVIVTCNPDVAKEILNSS+FADRPVKESAYSLMFNRAIGFAPYG+          T    
Sbjct: 121 RVIVTCNPDVAKEILNSSVFADRPVKESAYSLMFNRAIGFAPYGVYWRTLRKIAATHLFC 180

Query: 183 PS--KSSPPSPS------------------AAKSLPKWSQFYDLS----------DSANN 242
           P   K+S    S                  + + L K +   ++           DS+N 
Sbjct: 181 PKQIKASEAQRSEIASQMVAMMLGEQGKSVSVRQLLKRASLNNMMCSVFGCKYTVDSSNE 240

Query: 243 DVQELQSLVDEGYDLLGLLNWSDHLPWLADFDSQKIRFRCSRLVPKVNHFVGRIIAEHRA 302
           +V++L++LV EGY+LLGLLNWSDHLPWLA+FD+QKIRFRCS+LVPKVN FV RIIA+HRA
Sbjct: 241 EVEQLEALVGEGYELLGLLNWSDHLPWLAEFDAQKIRFRCSKLVPKVNRFVSRIIAQHRA 300

Query: 303 KSDNQVLDFVDVLLSLQEADKLSDSDMIAVLWVRNDFSWDGHGGSFNEWILARMVLHNDI 362
           +S  Q  DFVDVLLSLQ  + LS+SDMIAVLW    F          EWILARMVLH D+
Sbjct: 301 QSSEQSRDFVDVLLSLQGPESLSESDMIAVLW-EMIFRGTDTVAVLIEWILARMVLHLDV 360

Query: 363 QRKVQEELDNVVGSTRAVAESDIPSLVYLTAVVKEVLRLHPPGPLLSWARLAITDTIIDG 422
           Q KV EELD VVG +RAVAE+D+ SLVY+ AVVKEVLRLHPPGPLLSWARLA+T+TIIDG
Sbjct: 361 QSKVHEELDKVVGISRAVAEADVASLVYMAAVVKEVLRLHPPGPLLSWARLAVTNTIIDG 420

Query: 423 HHVPRGTTAMVNMWSIARDPQVWSDPLEFMPQRFVSDPGDVEFSVMGSDLRLAPFGSGRR 450
           +HVP+GTTAMVNMW+IARDP+VWSDPL+FMP+RFV+  G++EFS++GSDLRLAPFGSGRR
Sbjct: 421 YHVPQGTTAMVNMWAIARDPEVWSDPLKFMPERFVAKEGELEFSILGSDLRLAPFGSGRR 480

BLAST of Sgr011886 vs. ExPASy TrEMBL
Match: A0A6J1HNC6 (cytochrome P450 78A3-like OS=Cucurbita moschata OX=3662 GN=LOC111464500 PE=3 SV=1)

HSP 1 Score: 581.3 bits (1497), Expect = 4.0e-162
Identity = 312/485 (64.33%), Postives = 358/485 (73.81%), Query Frame = 0

Query: 1   MSMSSEIESLWVFALASKCSALTKENILWSLLFFFLIWVSVSILHWAHPGGPAWGRYWWR 60
           MS+S+EIESLWVFALASKCS LT+ NIL+  LF  LIW  VSI HWAHPGGPAWGR+   
Sbjct: 1   MSLSTEIESLWVFALASKCSPLTQPNILYFFLFLCLIWFLVSIFHWAHPGGPAWGRHPRC 60

Query: 61  RRRS--NSTAAAIPGPRGLPLVGSMGLMADLAHHRIAAVADSLNATRLMAFSLGDTRVIV 120
           RR S  ++T   IPGPRGLP++GSM LM  LAHHRIAA+A SL ATRLMAFSL +TRVIV
Sbjct: 61  RRNSPLSTTTTTIPGPRGLPVIGSMTLMTGLAHHRIAAMAKSLGATRLMAFSLANTRVIV 120

Query: 121 TCNPDVAKEILNSSLFADRPVKESAYSLMFNRAIGFAPYGL------------------- 180
           TCNPDVAKEILNSS+FADRP+KESAYSLMFNRAIGFAPY +                   
Sbjct: 121 TCNPDVAKEILNSSVFADRPIKESAYSLMFNRAIGFAPYDVYWRTLRRIASQHLFCPKQI 180

Query: 181 ---------TGGPSAASLPTTSSAPSKSSPPSPSAAKSL-----PKWSQFYDLSDSANND 240
                          A     ++  S+          SL       + Q Y+LSDS NN+
Sbjct: 181 KSSEFQRRQIASQMVAIFANNNNTSSRHQVRDTLKRASLNHMMGSVFGQAYNLSDS-NNE 240

Query: 241 VQELQSLVDEGYDLLGLLNWSDHLPWLADFDSQKIRFRCSRLVPKVNHFVGRIIAEHRAK 300
           +++L+SLVDEGYDLLGLLNWSDHLP+L D D Q+IRFRCSRLVPKVN F+ RIIA+HR+K
Sbjct: 241 IEQLKSLVDEGYDLLGLLNWSDHLPFLGDLDPQRIRFRCSRLVPKVNRFLNRIIADHRSK 300

Query: 301 SDNQVLDFVDVLLSLQEADKLSDSDMIAVLWVRNDFSWDGHGGSFNEWILARMVLHNDIQ 360
           S  Q++DFVDVLLSLQ+ + LSDSD+IAVLW    F          EW LARMV+H + Q
Sbjct: 301 STQQIMDFVDVLLSLQQTENLSDSDIIAVLW-EMIFRGTDTVAVLIEWTLARMVVHKEEQ 360

Query: 361 RKVQEELDNVVGSTRAVAESDIPSLVYLTAVVKEVLRLHPPGPLLSWARLAITDTIIDGH 420
            KVQEELD VVG +RAV ESDIPSL YLTAVVKEVLRLHPPGPLLSWARLA +DT++DGH
Sbjct: 361 EKVQEELDKVVGRSRAVVESDIPSLEYLTAVVKEVLRLHPPGPLLSWARLARSDTMVDGH 420

Query: 421 HVPRGTTAMVNMWSIARDPQVWSDPLEFMPQRFVSDP-GDVEFSVMGSDLRLAPFGSGRR 450
           HVPRGTTAMVNMWSIARDP+VW  P EFMP+RFV +    VEF +MGSDLRLAPFGSGRR
Sbjct: 421 HVPRGTTAMVNMWSIARDPEVWWSPQEFMPERFVGNKCCGVEFPIMGSDLRLAPFGSGRR 480

BLAST of Sgr011886 vs. TAIR 10
Match: AT3G61880.1 (cytochrome p450 78a9 )

HSP 1 Score: 511.1 bits (1315), Expect = 9.7e-145
Identity = 282/471 (59.87%), Postives = 327/471 (69.43%), Query Frame = 0

Query: 12  VFALASKCSALTKENILWSLLFFFLIWVSVSILHWAHPGGPAWGRYWWRRRRSNSTAAAI 71
           + AL SKCS LT+ N+  SLL   L  +++S+  W+HPGGPAWG+Y+  RRR  +    I
Sbjct: 11  LLALLSKCSLLTQTNLALSLLVASLASLALSLFFWSHPGGPAWGKYFLHRRRQTT---VI 70

Query: 72  PGPRGLPLVGSMGLMAD-LAHHRIAAVADSLNATRLMAFSLGDTRVIVTCNPDVAKEILN 131
           PGPRGLP VGSM LM++ LAH  IAA A+   A RLMAFSLG+TRVIVTCNPDVAKEILN
Sbjct: 71  PGPRGLPFVGSMSLMSNTLAHRCIAATAEKFRAERLMAFSLGETRVIVTCNPDVAKEILN 130

Query: 132 SSLFADRPVKESAYSLMFNRAIGFAPYGL-------TGGPSAASLPTTSSAPSKSSPPSP 191
           S +FADRPVKESAYSLMFNRAIGFAPYG+              S      + ++ S  + 
Sbjct: 131 SPVFADRPVKESAYSLMFNRAIGFAPYGVYWRTLRKIASNHLFSPKQIKRSETQRSVIAN 190

Query: 192 SAAKSLPKWSQFYDL--------SDSANN-----------------DVQELQSLVDEGYD 251
              K L K S    L        + S NN                 +V EL+ LV+EGYD
Sbjct: 191 QIVKCLTKQSNTKGLCFARDLIKTASLNNMMCSVFGKEYELEEEHEEVSELRELVEEGYD 250

Query: 252 LLGLLNWSDHLPWLADFDSQKIRFRCSRLVPKVNHFVGRIIAEHRAKSDNQVLDFVDVLL 311
           LLG LNW+DHLPWL++FD Q+IR RCS LVPKVN FV RII++HR ++ +   DFVDVLL
Sbjct: 251 LLGTLNWTDHLPWLSEFDPQRIRSRCSNLVPKVNRFVNRIISDHREQTRDSPSDFVDVLL 310

Query: 312 SLQEADKLSDSDMIAVLWVRNDFSWDGHGGSFNEWILARMVLHNDIQRKVQEELDNVVGS 371
           SL   DKLSD D+IAVLW    F          EWILARMVLH DIQ  V  ELD +VG 
Sbjct: 311 SLDGPDKLSDPDIIAVLW-EMIFRGTDTVAVLIEWILARMVLHPDIQSTVHNELDQIVGR 370

Query: 372 TRAVAESDIPSLVYLTAVVKEVLRLHPPGPLLSWARLAITDTIIDGHHVPRGTTAMVNMW 431
           +RAV ESD+ SLVYLTAVVKEVLRLHPPGPLLSWARLAITDTIIDG  VP GTTAMVNMW
Sbjct: 371 SRAVEESDVVSLVYLTAVVKEVLRLHPPGPLLSWARLAITDTIIDGRRVPAGTTAMVNMW 430

Query: 432 SIARDPQVWSDPLEFMPQRFVSDPGDVEFSVMGSDLRLAPFGSGRRTCPGR 450
           +IA DP VW +PLEF P+RFV+  G+VEFSV+GSDLRLAPFGSGRR CPG+
Sbjct: 431 AIAHDPHVWENPLEFKPERFVAKEGEVEFSVLGSDLRLAPFGSGRRVCPGK 477

BLAST of Sgr011886 vs. TAIR 10
Match: AT3G61880.2 (cytochrome p450 78a9 )

HSP 1 Score: 511.1 bits (1315), Expect = 9.7e-145
Identity = 282/471 (59.87%), Postives = 327/471 (69.43%), Query Frame = 0

Query: 12  VFALASKCSALTKENILWSLLFFFLIWVSVSILHWAHPGGPAWGRYWWRRRRSNSTAAAI 71
           + AL SKCS LT+ N+  SLL   L  +++S+  W+HPGGPAWG+Y+  RRR  +    I
Sbjct: 11  LLALLSKCSLLTQTNLALSLLVASLASLALSLFFWSHPGGPAWGKYFLHRRRQTT---VI 70

Query: 72  PGPRGLPLVGSMGLMAD-LAHHRIAAVADSLNATRLMAFSLGDTRVIVTCNPDVAKEILN 131
           PGPRGLP VGSM LM++ LAH  IAA A+   A RLMAFSLG+TRVIVTCNPDVAKEILN
Sbjct: 71  PGPRGLPFVGSMSLMSNTLAHRCIAATAEKFRAERLMAFSLGETRVIVTCNPDVAKEILN 130

Query: 132 SSLFADRPVKESAYSLMFNRAIGFAPYGL-------TGGPSAASLPTTSSAPSKSSPPSP 191
           S +FADRPVKESAYSLMFNRAIGFAPYG+              S      + ++ S  + 
Sbjct: 131 SPVFADRPVKESAYSLMFNRAIGFAPYGVYWRTLRKIASNHLFSPKQIKRSETQRSVIAN 190

Query: 192 SAAKSLPKWSQFYDL--------SDSANN-----------------DVQELQSLVDEGYD 251
              K L K S    L        + S NN                 +V EL+ LV+EGYD
Sbjct: 191 QIVKCLTKQSNTKGLCFARDLIKTASLNNMMCSVFGKEYELEEEHEEVSELRELVEEGYD 250

Query: 252 LLGLLNWSDHLPWLADFDSQKIRFRCSRLVPKVNHFVGRIIAEHRAKSDNQVLDFVDVLL 311
           LLG LNW+DHLPWL++FD Q+IR RCS LVPKVN FV RII++HR ++ +   DFVDVLL
Sbjct: 251 LLGTLNWTDHLPWLSEFDPQRIRSRCSNLVPKVNRFVNRIISDHREQTRDSPSDFVDVLL 310

Query: 312 SLQEADKLSDSDMIAVLWVRNDFSWDGHGGSFNEWILARMVLHNDIQRKVQEELDNVVGS 371
           SL   DKLSD D+IAVLW    F          EWILARMVLH DIQ  V  ELD +VG 
Sbjct: 311 SLDGPDKLSDPDIIAVLW-EMIFRGTDTVAVLIEWILARMVLHPDIQSTVHNELDQIVGR 370

Query: 372 TRAVAESDIPSLVYLTAVVKEVLRLHPPGPLLSWARLAITDTIIDGHHVPRGTTAMVNMW 431
           +RAV ESD+ SLVYLTAVVKEVLRLHPPGPLLSWARLAITDTIIDG  VP GTTAMVNMW
Sbjct: 371 SRAVEESDVVSLVYLTAVVKEVLRLHPPGPLLSWARLAITDTIIDGRRVPAGTTAMVNMW 430

Query: 432 SIARDPQVWSDPLEFMPQRFVSDPGDVEFSVMGSDLRLAPFGSGRRTCPGR 450
           +IA DP VW +PLEF P+RFV+  G+VEFSV+GSDLRLAPFGSGRR CPG+
Sbjct: 431 AIAHDPHVWENPLEFKPERFVAKEGEVEFSVLGSDLRLAPFGSGRRVCPGK 477

BLAST of Sgr011886 vs. TAIR 10
Match: AT2G46660.1 (cytochrome P450, family 78, subfamily A, polypeptide 6 )

HSP 1 Score: 510.0 bits (1312), Expect = 2.2e-144
Identity = 278/478 (58.16%), Postives = 333/478 (69.67%), Query Frame = 0

Query: 3   MSSEIESLWVFALASKCSALTKENILWSLLFFFLIWVSVSILHWAHPGGPAWGRYWWRRR 62
           M++++ES  +FAL SKCS L++ N+ +SLL   +IW+++S+  W +PGGPAWG+Y + R 
Sbjct: 1   MATKLESSLIFALLSKCSVLSQTNLAFSLLAVTIIWLAISLFLWTYPGGPAWGKYLFGRL 60

Query: 63  RSNS--TAAAIPGPRGLPLVGSMGLMAD-LAHHRIAAVADSLNATRLMAFSLGDTRVIVT 122
            S S  T   IPGP+G PLVGSM LM+  LAH RIA  A+   A RLMAFSLG+TRVIVT
Sbjct: 61  ISGSYKTGNVIPGPKGFPLVGSMSLMSSTLAHRRIADAAEKFGAKRLMAFSLGETRVIVT 120

Query: 123 CNPDVAKEILNSSLFADRPVKESAYSLMFNRAIGFAPYG-------------LTGGPSAA 182
           CNPDVAKEILNS +FADRPVKESAYSLMFNRAIGFAP+G             L       
Sbjct: 121 CNPDVAKEILNSPVFADRPVKESAYSLMFNRAIGFAPHGVYWRTLRRIASNHLFSTKQIR 180

Query: 183 SLPTTSSAPS--------KSSPPSPSAAKSLPKWSQFYDLSDSA-------NNDVQELQS 242
              T     S        K S   P   + L K +   ++  S          +  EL+ 
Sbjct: 181 RAETQRRVISSQMVEFLEKQSSNEPCFVRELLKTASLNNMMCSVFGQEYELEKNHVELRE 240

Query: 243 LVDEGYDLLGLLNWSDHLPWLADFDSQKIRFRCSRLVPKVNHFVGRIIAEHRAKSDNQVL 302
           +V+EGYDLLG LNW+DHLPWL++FD Q++R RCS LVPKVN FV RII+EHR ++ +   
Sbjct: 241 MVEEGYDLLGTLNWTDHLPWLSEFDPQRLRSRCSTLVPKVNRFVSRIISEHRNQTGDLPR 300

Query: 303 DFVDVLLSLQEADKLSDSDMIAVLWVRNDFSWDGHGGSFNEWILARMVLHNDIQRKVQEE 362
           DFVDVLLSL  +DKLSD D+IAVLW    F          EWILARMVLH D+Q  VQ E
Sbjct: 301 DFVDVLLSLHGSDKLSDPDIIAVLW-EMIFRGTDTVAVLIEWILARMVLHPDMQSTVQNE 360

Query: 363 LDNVVGSTRAVAESDIPSLVYLTAVVKEVLRLHPPGPLLSWARLAITDTIIDGHHVPRGT 422
           LD VVG +RA+ ESD+ SL YLTAVVKEVLRLHPPGPLLSWARLAITDTI+DG  VP GT
Sbjct: 361 LDQVVGKSRALDESDLASLPYLTAVVKEVLRLHPPGPLLSWARLAITDTIVDGRLVPAGT 420

Query: 423 TAMVNMWSIARDPQVWSDPLEFMPQRFVSDPGDVEFSVMGSDLRLAPFGSGRRTCPGR 450
           TAMVNMW+++ DP VW DPLEF P+RFV+  G+VEFSV+GSDLRLAPFGSGRR CPG+
Sbjct: 421 TAMVNMWAVSHDPHVWVDPLEFKPERFVAKEGEVEFSVLGSDLRLAPFGSGRRICPGK 477

BLAST of Sgr011886 vs. TAIR 10
Match: AT1G01190.1 (cytochrome P450, family 78, subfamily A, polypeptide 8 )

HSP 1 Score: 484.6 bits (1246), Expect = 9.7e-137
Identity = 262/484 (54.13%), Postives = 328/484 (67.77%), Query Frame = 0

Query: 3   MSSEIESLWVFALASKCSALTKENILWSLLFFFLIWVSVSILHWAHPGGPAWGRYWWRRR 62
           M +EIESLWVFALASK +   +++    L+   + W +++I+ W+ PGGPAWG+Y++ RR
Sbjct: 1   MRTEIESLWVFALASKFNIYMQQHFASLLVAIAITWFTITIVFWSTPGGPAWGKYFFTRR 60

Query: 63  -----RSNSTAAAIPGPRGLPLVGSMGLMAD-LAHHRIAAVADSLNATRLMAFSLGDTRV 122
                 +      IPGPRG PLVGSM L +  +AH RIA+VA+  NA RLMAFSLGDT+V
Sbjct: 61  FISLDYNRKYKNLIPGPRGFPLVGSMSLRSSHVAHQRIASVAEMSNAKRLMAFSLGDTKV 120

Query: 123 IVTCNPDVAKEILNSSLFADRPVKESAYSLMFNRAIGFAPYGL----------------- 182
           +VTC+P VAKEILNSS+FADRPV E+AY LMFNRA+GFAP G                  
Sbjct: 121 VVTCHPAVAKEILNSSVFADRPVDETAYGLMFNRAMGFAPNGTYWRTLRRLGSNHLFNPK 180

Query: 183 ---TGGPSAASLPTTSSAPSKSSPPSPSAAKSLPK-----------WSQFYDLSDSANND 242
                      + T        +P S  A + L K           + + Y+L  + N +
Sbjct: 181 QIKQSEDQRRVIATQMVNAFARNPKSACAVRDLLKTASLCNMMGLVFGREYELESNNNLE 240

Query: 243 VQELQSLVDEGYDLLGLLNWSDHLPWLADFDSQKIRFRCSRLVPKVNHFVGRIIAEHRAK 302
            + L+ LV+EGYDLLG LNW+DHLPWLA  D Q+IRFRCS+LVPKVN  + RII E RA 
Sbjct: 241 SECLKGLVEEGYDLLGTLNWTDHLPWLAGLDFQQIRFRCSQLVPKVNLLLSRIIHEQRAA 300

Query: 303 SDNQVLDFVDVLLSLQEADKLSDSDMIAVLWVRNDFSWDGHGGSFNEWILARMVLHNDIQ 362
           + N    F+D+LLSLQ ++KLS+SDM+AVLW    F          EW+LAR+V+H  +Q
Sbjct: 301 TGN----FLDMLLSLQGSEKLSESDMVAVLW-EMIFRGTDTVAVLVEWVLARIVMHPKVQ 360

Query: 363 RKVQEELDNVVGSTRAVAESDIPSLVYLTAVVKEVLRLHPPGPLLSWARLAITDTIIDGH 422
             V +ELD VVG +R V ESD+PSL YLTA++KEVLRLHPPGPLLSWARL+ITDT +DG+
Sbjct: 361 LTVHDELDRVVGRSRTVDESDLPSLTYLTAMIKEVLRLHPPGPLLSWARLSITDTSVDGY 420

Query: 423 HVPRGTTAMVNMWSIARDPQVWSDPLEFMPQRFVSDPGDVEFSVMGSDLRLAPFGSGRRT 450
           HVP GTTAMVNMW+IARDP VW DPLEF P+RFV+  G+ EFSV GSDLRLAPFGSG+R 
Sbjct: 421 HVPAGTTAMVNMWAIARDPHVWEDPLEFKPERFVAKEGEAEFSVFGSDLRLAPFGSGKRV 479

BLAST of Sgr011886 vs. TAIR 10
Match: AT1G74110.1 (cytochrome P450, family 78, subfamily A, polypeptide 10 )

HSP 1 Score: 351.3 bits (900), Expect = 1.3e-96
Identity = 214/485 (44.12%), Postives = 273/485 (56.29%), Query Frame = 0

Query: 13  FALASKCSALTKENILWSLLFFFLIWVSVSILHWAHPGGPAWGRYWWR--RRRSNSTAAA 72
           FA  S  S      +  S+  F  ++V      W  PGG AW  Y  R   R  + T  A
Sbjct: 9   FASRSGSSPFPSLELCLSIFLFISLFV-----FWLTPGGFAWALYKARFHTRPESKTGPA 68

Query: 73  IPGPRGLPLVGSMGLMAD--LAHHRIAAVADSLNATRLMAFSLGDTRVIVTCNPDVAKEI 132
           IPGP GLP+ G +    +  L H  +A +AD+  A  LMAFS+G TR ++T  P+ AKE+
Sbjct: 69  IPGPSGLPIFGLLLAFVNNALTHRILANIADTCKAKALMAFSVGSTRFVITSEPETAKEL 128

Query: 133 LNSSLFADRPVKESAYSLMFNRAIGFAPYG--------------------LTGGPSAASL 192
           LNSS FADRPVKESAY L+F+RA+GFAP+G                     + G S   +
Sbjct: 129 LNSSAFADRPVKESAYELLFDRAMGFAPFGDYWRELRRISSTHLFSPKRIFSSGESRRKI 188

Query: 193 PTTSSAPSKSSPPSPSAA--KSLPKWSQFYDLSDSA-------------NNDVQELQSLV 252
                   K++         K +  +    ++  S              + +  EL+ LV
Sbjct: 189 GQNMVGEIKNAMECYGEVHIKKILHFGSLNNVMSSVFGKTYNFNEGIVYSKESNELEHLV 248

Query: 253 DEGYDLLGLLNWSDHLPWLADFDSQKIRFRCSRLVPKVNHFVGRIIAEHRAK---SDNQV 312
            EGY+LLG+ NWSDH P +   D Q +R RC  LV +VN FVG+II +H++K    DN  
Sbjct: 249 SEGYELLGIFNWSDHFPGMRWLDLQGVRRRCRSLVGRVNVFVGKIINDHKSKRSLRDNPE 308

Query: 313 L-----DFVDVLLSLQEADKLSDSDMIAVLWVRNDFSWDGHGGSFNEWILARMVLHNDIQ 372
                 DFVDVLL +    KLSDSDMIAVLW    F          EWILARMVLH DIQ
Sbjct: 309 ESTYDDDFVDVLLGMHGNSKLSDSDMIAVLW-EMIFRGTDTVAILLEWILARMVLHPDIQ 368

Query: 373 RKVQEELDNVVG-STRAVAESDIPSLVYLTAVVKEVLRLHPPGPLLSWARLAITDTIIDG 432
            K Q E+D +VG S R V +SD+P L Y+ A+VKE LR+HPPGPLLSWARL+I DT I  
Sbjct: 369 AKAQAEIDCIVGDSGRQVTDSDLPKLPYVRAIVKETLRMHPPGPLLSWARLSIHDTQIGT 428

Query: 433 HHVPRGTTAMVNMWSIARDPQVWSDPLEFMPQRFVSDPGDVEFSVMGSDLRLAPFGSGRR 450
           H +P GTTAMVNMW+I  D +VW +  E+ P+RF+       F +MGSDLRLAPFG+GRR
Sbjct: 429 HFIPAGTTAMVNMWAITHDEKVWPEAHEYKPERFLGAQESNNFPIMGSDLRLAPFGAGRR 487

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038875232.14.5e-16866.80cytochrome P450 78A3-like [Benincasa hispida][more]
XP_023006561.11.4e-16666.94cytochrome P450 78A3-like [Cucurbita maxima][more]
XP_022999908.14.6e-16563.88cytochrome P450 78A3-like [Cucurbita maxima][more]
XP_022959466.16.0e-16567.01cytochrome P450 78A3 [Cucurbita moschata][more]
KAG6575238.11.3e-16466.80Cytochrome P450 78A3, partial [Cucurbita argyrosperma subsp. sororia] >KAG701380... [more]
Match NameE-valueIdentityDescription
O489279.4e-14558.46Cytochrome P450 78A3 OS=Glycine max OX=3847 GN=CYP78A3 PE=2 SV=1[more]
Q9SLP11.4e-14359.87Cytochrome P450 78A9 OS=Arabidopsis thaliana OX=3702 GN=CYP78A9 PE=1 SV=1[more]
Q9ZNR03.0e-14358.16Cytochrome P450 78A6 OS=Arabidopsis thaliana OX=3702 GN=CYP78A6 PE=2 SV=1[more]
O650121.3e-9344.90Cytochrome P450 78A4 OS=Pinus radiata OX=3347 GN=CYP78A4 PE=2 SV=1[more]
Q9FIB07.8e-9142.74Cytochrome P450 78A7 OS=Arabidopsis thaliana OX=3702 GN=CYP78A7 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1KY337.0e-16766.94cytochrome P450 78A3-like OS=Cucurbita maxima OX=3661 GN=LOC111499249 PE=3 SV=1[more]
A0A6J1KL152.2e-16563.88cytochrome P450 78A3-like OS=Cucurbita maxima OX=3661 GN=LOC111494245 PE=3 SV=1[more]
A0A6J1H4X92.9e-16567.01cytochrome P450 78A3 OS=Cucurbita moschata OX=3662 GN=LOC111460432 PE=3 SV=1[more]
A0A2N9IN334.2e-16463.09Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS54868 PE=3 SV=1[more]
A0A6J1HNC64.0e-16264.33cytochrome P450 78A3-like OS=Cucurbita moschata OX=3662 GN=LOC111464500 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
AT3G61880.19.7e-14559.87cytochrome p450 78a9 [more]
AT3G61880.29.7e-14559.87cytochrome p450 78a9 [more]
AT2G46660.12.2e-14458.16cytochrome P450, family 78, subfamily A, polypeptide 6 [more]
AT1G01190.19.7e-13754.13cytochrome P450, family 78, subfamily A, polypeptide 8 [more]
AT1G74110.11.3e-9644.12cytochrome P450, family 78, subfamily A, polypeptide 10 [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PRINTSPR00385P450coord: 320..333
score: 37.42
coord: 355..366
score: 48.01
coord: 437..446
score: 55.37
IPR001128Cytochrome P450PFAMPF00067p450coord: 205..449
e-value: 2.7E-48
score: 164.9
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 311..337
score: 35.48
coord: 436..446
score: 52.14
coord: 354..372
score: 35.04
coord: 396..420
score: 37.39
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 57..160
e-value: 1.1E-9
score: 39.3
coord: 194..451
e-value: 1.1E-57
score: 197.7
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 57..449
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 166..188
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 440..495
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 458..495
NoneNo IPR availablePANTHERPTHR47946CYTOCHROME P450 78A7-RELATEDcoord: 193..449
coord: 3..159
NoneNo IPR availablePANTHERPTHR47946:SF9CYTOCHROME P450 78A6coord: 193..449
coord: 3..159
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 439..448

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr011886.1Sgr011886.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016020 membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen