Sgr011755 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr011755 vs. NCBI nr
Match: KAG6572151.1 (putative pectinesterase/pectinesterase inhibitor 7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 157.5 bits (397), Expect = 1.4e-34
Identity = 89/186 (47.85%), Postives = 106/186 (56.99%), Query Frame = 0

Query: 2   ASPPFLVFFTLLFVSIVNLSMSVKADQNTTINNFAHAICNRIQYNELYKVCIPEIDSDPR 61
           +S  FL  FTLL +SI N SMS K ++  T + F H  CN+ Q  +LYK+CIPEIDSDPR
Sbjct: 308 SSLTFLGAFTLLLLSIFNPSMSAKVEE--TRSTFVHDTCNKFQIEQLYKLCIPEIDSDPR 367

Query: 62  EDLKSNLT-------------------------------------------DFERGIGDL 121
           EDLKSNLT                                           DFERG GDL
Sbjct: 368 EDLKSNLTGILIIFVNHSFSNFNDNIAFLQREIKSGKLNGETKVMYNGCLEDFERGNGDL 427

Query: 122 QESLQILLTKKGIDVEQLPATNVGNYVSGCVEDFEGVPVPPEWQSRYNTSWALLDLSLVM 145
           QE++ ILLT+ GID   LP  N+ NYVS CV+ +EG P+PPEWQSRYNTSW LL L  V 
Sbjct: 428 QETMHILLTQTGIDAYNLPTINIPNYVSECVDFYEGDPIPPEWQSRYNTSWNLLTLIGVT 487

BLAST of Sgr011755 vs. NCBI nr
Match: KAG6572152.1 (Expansin-like A2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 139.0 bits (349), Expect = 5.3e-29
Identity = 78/187 (41.71%), Postives = 102/187 (54.55%), Query Frame = 0

Query: 2   ASPPFL-VFFTLLFVSIVNLSMSVKADQNTTINNFAHAICNRIQYNELYKVCIPEIDSDP 61
           ++PP    F     +++  +   V     +T NNFAH  CNR Q+ +LYK+CIPE+DS+P
Sbjct: 121 STPPLCHPFINKELLAVPVIKFVVAEVNRSTNNNFAHDTCNRFQFEQLYKLCIPEVDSEP 180

Query: 62  REDLKSNLT-------------------------------------------DFERGIGD 121
           REDLKSNLT                                           +FERG  D
Sbjct: 181 REDLKSNLTGILIIFVNHTISNFKDNVMFLQREIKSGKLNGKTKEMYDTCLENFERGTTD 240

Query: 122 LQESLQILLTKKGIDVEQLPATNVGNYVSGCVEDFEGVPVPPEWQSRYNTSWALLDLSLV 145
           LQ+S+ +LLT+ G D+  LPA +V +YV+ CV+DFEGVP PPEWQSRYNTS  LL+L L 
Sbjct: 241 LQQSMHVLLTETGKDIYGLPALSVASYVTECVDDFEGVPKPPEWQSRYNTSNKLLELILG 300

BLAST of Sgr011755 vs. NCBI nr
Match: KAG6572380.1 (ATP-dependent zinc metalloprotease FTSH 2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 136.0 bits (341), Expect = 4.5e-28
Identity = 74/166 (44.58%), Postives = 92/166 (55.42%), Query Frame = 0

Query: 22  MSVKADQNTTIN-NFAHAICNRIQYNELYKVCIPEIDSDPREDLKSNLTD---------- 81
           M   A++N + N NF H  CN+ QY+ELYKVCIPEIDSDPREDLKSNLT           
Sbjct: 1   MFAMAEENRSSNCNFVHDTCNKFQYDELYKVCIPEIDSDPREDLKSNLTGFLIIFVNHTI 60

Query: 82  ---------------------------------FERGIGDLQESLQILLTKKGIDVEQLP 141
                                            F  G  +LQES+ ILLT+ G +V +LP
Sbjct: 61  SNFNDNIVFLQREIQSGKLNAETKLMYNTCLKAFGMGYSNLQESMHILLTQPGTNVYELP 120

Query: 142 ATNVGNYVSGCVEDFEGVPVPPEWQSRYNTSWALLDLSLVMTNLIT 144
             +V   +S CV+DF+G P+PPEWQSRYNTSW L+ L +  +NLIT
Sbjct: 121 TMDVDKRISTCVDDFDGEPIPPEWQSRYNTSWNLVALMVETSNLIT 166

BLAST of Sgr011755 vs. NCBI nr
Match: KAA0055284.1 (putative invertase inhibitor [Cucumis melo var. makuwa] >TYJ99207.1 putative invertase inhibitor [Cucumis melo var. makuwa])

HSP 1 Score: 135.6 bits (340), Expect = 5.9e-28
Identity = 77/176 (43.75%), Postives = 97/176 (55.11%), Query Frame = 0

Query: 12  LLFVSIVNLSMSVKADQNTTINNFAHAICNRIQYNELYKVCIPEIDSDPREDLKSNLT-- 71
           L+ + I++L M  KADQNTTIN+F    CNR   +EL  +C+ +I S+PR DLKSNLT  
Sbjct: 8   LVLLCILSLCMLTKADQNTTINDFVRHTCNRT--HELKNLCLSQISSEPRNDLKSNLTGL 67

Query: 72  -----------------------------------------DFERGIGDLQESLQILLTK 131
                                                    +F+ G  +L+E+++IL TK
Sbjct: 68  LMIFVNQSISNFKNDISFLEKEINSNKISRDTKDMLEDCLQNFQIGSVNLRETMEILQTK 127

Query: 132 KGIDVEQLPATNVGNYVSGCVEDFEGVPVPPEWQSRYNTSWALLDLSLVMTNLITC 145
            G D EQLP  NVGN    C +DFEG PVPPEWQSRYN S+ALL LS+ M NLITC
Sbjct: 128 TGYDAEQLPVGNVGNLAVECFDDFEGTPVPPEWQSRYNASFALLKLSMAMINLITC 181

BLAST of Sgr011755 vs. NCBI nr
Match: XP_023511468.1 (uncharacterized protein LOC111776286 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 125.9 bits (315), Expect = 4.7e-25
Identity = 69/161 (42.86%), Postives = 82/161 (50.93%), Query Frame = 0

Query: 27  DQNTTINNFAHAICNRIQYNELYKVCIPEIDSDPREDLKSNLT----------------- 86
           +  +T + F H  CNR QYNELYK+CIPEID DPREDLKSNLT                 
Sbjct: 4   EDKSTDDTFVHDTCNRFQYNELYKLCIPEIDLDPREDLKSNLTGFLTIFVNHTINNFNDN 63

Query: 87  --------------------------DFERGIGDLQESLQILLTKKGIDVEQLPATNVGN 145
                                      F  G   LQES+ ILLT+ G +V  LP  +V  
Sbjct: 64  LIFLQREIKSGKLDAETKLMYDKCSESFGMGYTKLQESMHILLTQPGTNVYDLPTMSVDK 123

BLAST of Sgr011755 vs. ExPASy TrEMBL
Match: A0A5D3BJB5 (Putative invertase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G004170 PE=4 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 2.9e-28
Identity = 77/176 (43.75%), Postives = 97/176 (55.11%), Query Frame = 0

Query: 12  LLFVSIVNLSMSVKADQNTTINNFAHAICNRIQYNELYKVCIPEIDSDPREDLKSNLT-- 71
           L+ + I++L M  KADQNTTIN+F    CNR   +EL  +C+ +I S+PR DLKSNLT  
Sbjct: 8   LVLLCILSLCMLTKADQNTTINDFVRHTCNRT--HELKNLCLSQISSEPRNDLKSNLTGL 67

Query: 72  -----------------------------------------DFERGIGDLQESLQILLTK 131
                                                    +F+ G  +L+E+++IL TK
Sbjct: 68  LMIFVNQSISNFKNDISFLEKEINSNKISRDTKDMLEDCLQNFQIGSVNLRETMEILQTK 127

Query: 132 KGIDVEQLPATNVGNYVSGCVEDFEGVPVPPEWQSRYNTSWALLDLSLVMTNLITC 145
            G D EQLP  NVGN    C +DFEG PVPPEWQSRYN S+ALL LS+ M NLITC
Sbjct: 128 TGYDAEQLPVGNVGNLAVECFDDFEGTPVPPEWQSRYNASFALLKLSMAMINLITC 181

BLAST of Sgr011755 vs. ExPASy TrEMBL
Match: A0A6J1C7W5 (uncharacterized protein LOC111008804 OS=Momordica charantia OX=3673 GN=LOC111008804 PE=4 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 3.6e-07
Identity = 47/164 (28.66%), Postives = 69/164 (42.07%), Query Frame = 0

Query: 24  VKADQNTTINNFAHAICNRIQYNELYKVCIPEIDSDPREDLKSNLT-------------- 83
           ++ ++ +  N+F +  CN+ +  E  KVCI  IDSDP +DLK NL+              
Sbjct: 2   MRVEKLSVANSFVNNTCNKFE-EEFIKVCIDVIDSDPHQDLKLNLSGLLAIFINHSIDNA 61

Query: 84  -----------------------------DFERGIGDLQESLQILLTKKGIDVEQLPATN 143
                                         +ERG+  L+ SL+ LL K   D   L  ++
Sbjct: 62  TDNHLYLMEQLKRSDLDKDIRYTFNVCVEQYERGLDILKSSLEALL-KNDKDDLGLILSD 121

Query: 144 VGNYVSGCVEDFEGVPVPPEWQSRYNTSWALLDLSLVMTNLITC 145
             + +  C +DFE  P PP W + Y     LL LS+   NLI C
Sbjct: 122 AFDKIVACEDDFESTPEPPSWLAHYLPLRKLLALSMGTANLIQC 163