Sgr011741 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr011741
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
DescriptionL-ascorbate oxidase
Locationtig00153049: 87309 .. 92546 (+)
RNA-Seq ExpressionSgr011741
SyntenySgr011741
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATATTGCTGCAGAGCCATTTCGGAGAGGTTTTCGAGTCCTTTTGGTTATATTCTTCCTTTTGGTTTCTGGGTTTGGTTTGTCTGAAGGTTCCAAAACCAGACACTTCCAATGGGATGTAGAGTACATGTTCTGGTCTCCAGATTGCGTAGAGAATGTGGTTATGGGGATCAACGGCCAGTTTCCCGGTCCGACGATCAGAGCCAATGTCGGAGACACCGTCGTCGTCGATGTCACGAACAAGCTTTTCACCGAAGGCGTCGTCATTCACTGGCATGGAATCAGGCAGATCGGAACTCCGTGGTCTGATGGCACAGCTTCGATCTCGCAGTGTGCCATCAACCCGGGAGAAACCTTTGTTTATAGATTCAAAGTTGATAAGGTGAGAACCATTTTTTTTTTTCATTTAAAGAACTTAATTCTCTCACTCGAAAACTTTCAAATGTATGTTTTGATTGATGAAGTTTGATGATATAAACATAAACATTGGTGGTGTGGGTGTGGGTGTGGACTTTTGTTATTATTAATGTTGTATTGTTGTGGTTTGGATGGGATAATTTGCAGGCAGGAACGTACTTCTATCACGGGCATTATGGGATGCAAAGATCAGCAGGGTTGTATGGATCTTTGATAGTGGACGTGGCAGAAGGAGAGAAAGAGCCATTTCATTACGACCAAGAGATCAACTTGTTGCTTAGTGATTGGTGGCATCAAAGTGTTCATGAACAAGAGGTGGGCCTCAGCTCCAGTCCAACTCGATGGATCGGCAACCCTCAGGTATTATTTATGTATACATCCAATTTCATAGACAATGAATATTTTATGTAGGCACTCTTTAACTTTAGCTTTTGAAATGAATAATAATGATTGCAGAGTTTGTTGATAAATGGAAGAGGTCAGTTCAAGTGCTCAATTGCAGCTAAGTACAATATTAGCTTGAACCAATGCAAATTGAAGGGAGGTGAACAATGCGCACCGTTTATCCTCCAAGTCCACCCCAATAAGACTTATAGGCTCAGAATTGCAAGTACCACTTCCTTGGCTTTCCTCAACGTCGCCATTGGGGTACTCTCTCTCTCTCTCTCTCTTTCTCATTTCCATTGCTTCTATATTAACTCAATTTGTATTAATTTTACTTTTAAATTATTTATATATTTTTTTTAAGTTTTAAGTTTTTAACCCACTTTTTTTTGTTTTTTTAATTGTCATTCATAGAATCACATCATGACGGTGGTAGAAGCCGACGGAAACTATGTCCAACCATTTAAGACGACCGACATCGACATTTATTCCGGCGAGAGCTACTCCGTCCTCATAACCACCAATCAAGACCCATCCAACAACTACTGGGTTTCCGCCGGCGTCAGAGGGCGAAAACCGGGGACCCCGCCGGCACTCACCCTCCTCAACTACCTCCCCAACTCCGCCTCCAAGTTGCCTGCTTCTCCGCCGCCGGTCACTCCCCGGTGGGACGACTTCGACCGCAGCAAGAACTTCTCCAAACAAATCTTCGCCGCCGTCGGCTCTGCGCAACCGCCAAGAATTTCAGCCGCCGGATCTTCCTTCTCAACACCCAGAACCAGATCAATGGTGTACAAAAGTGGGCTATGAACAACGTCTCCTTGGCGCTGCCGACGACGCCGTATCTCGGCGCCATCAAGTTCGGCTTAAACCAGTCTTTCGACCAAAGCAATCCGCCGGAGACTTTCCCACAGGATTACGACATCAACCGCCCTCCACCCAATCCCGCCACCACCATCGGAAACGCGATTTACCGCCTGGATCTGGGATCCACGGTGGACTTCATACTTCAGAACGCAAACACTTTGACCAAAAATTTGAGCGAGATCCACCCCTGGCATTTGCATGGCCACGACTTCTGGGTGTTGGGCTACGGAGAAGGAAAGTTCTCCGCCGCTGCCGACGAGAAGAAGCTCAACTTGAAGAACCCACCTCTGAAGAACACGGTGGTGATCTTCCCTTACGGATGGACGGCGGTGAGGTTCGTGGCGGACAACCCCGGCGTTTGGATCTTCCATTGCCATATCGAACCTCACCTGCATATGGGGATGGGTGTTATCTTCGCAGAAGGCGTCGATAAAGTCGGTGAGATTCCCAGGAGTGCTCTCTCGTGTGGGCTCACCGGACAAATGTCAATGGCGGACAAACCCTAAGAACTCTCTCACAGTAATATTTCGCCTGCAATAATGTGGTGCTTTCTGGGTCGTCTTCAACGATTGGGAAGTTCTGCAATCATTTTAACGGGCGTTTGGGTTTATGAATCTAATTTTATGCAGTAGTAATGTATGGTTGCCCTTTTATCATTTGTGTGCAATTTTCTTATTTTCTTTAGGTTAAATTATAAGTTTCGTACCCAACATTGATAAGACTTAGCATGAAGTGTCACGAGGAAGAATAAAATAAAGTTGGATTGTCACGAGGAAGTATGCCAATTTGCCCTTATATTATCTTGTTAGCATTTTTCTTTTTTCTAGTCATCATTGGTTACCTTAATCATTATATGTCACATTAATATTTAAAATATTAAAGACAAGGGCTTATGAACCGGTTAAGACATTATTTACCTTTTTAAAGGTCTAAGTTGGAATCTCCACCCCTTGTTTATTGAACTTAAAAAAAAAAAAAAAAAAGAGAGAGACAAGGGCTTATTAGAATCAAAATTGAAAATTCACATTTTTGGTCTTTGAGGTTTAGATTCAGTTTTTCTATTTGGTTCTTAGATTTTAAAAGCAACATTTTAGACTCTAAGGTGTATGGAAAGGTGACTGAACCCCACATCATTCACACTATAAATTTTTCTTCATAAATAATTGAAAGTTGTTAAGGATACAATAGATTAAGCATTTTAATAGAAACTATAGTTTTATTTTTTTATTTTTATTATTATTATTTTGAGTTCAACAAGAGGTGGAAGAGTGGGAATTCAAACCTTTGAGCTTTAAGGAGGTTATTGGGTGTCTTAACCAATTAAACTATGTTTGGTATAACAACTACAGTTTTATTAACAAATCAAAAAATTTGAAATAAGATTAAAACTTTGAAGACATTAAGGATAAACCTATATTGTGCATTTTAATAGAAATTTCAAGTTTTTAAATCAATAAAAAAAAAGTATGGATGGAATTGAAACTTTTACGTAAACCTAGATTAAGCATTTTAAAACAAATTCAAATTCTTTTCAATTAATCAAAAAGATTAAAAATGAAATTGACACCTTGAAAAGGTAAAGATTAGACTTGAATTAAGCATTTAATAAAATTGTATTTTCATTTTTTGAGTTCAAATTGCATTTTTTTTTTTAACAGATAAAAGATTAGAGATAAAATTGTAACATTTAAAATAAGCATTTAATAAAATTTCATTTTTTTAACAATAAAAAAGTTTATAGGTAGAATTAAAAAATTTAAATCAAACATATATTTTAATAGAAATTGAAGTTTTTTTAAACAAACCAAAAAACGATAGAATTGGAAGGATAAACCATTGATTAACGACTTCATCTATAAACTAATCAGAAAAATTATAATAATCTTATATTCAAATTTTTATAAACAATCTATATACCATTTCCTTCGAATTAGAATTTCATTGGAACTAAGAATTGTTGTATAATTAAAAAAATAATAGCGTAAAACATAATTCCTTTTGAGATAGAGTTTTTTTTTTTTTAGAAGAAGGTAGAGTTTTATTTAAAACTTAAGTAAAATGTTTATTTGATTGCAACTAAAATTATTTTAAAAACAAAAATGTACATACCACGAATTAGAATTCCATTATATTGTAACAAGAATTGTTTATAAAATTAGAATTTACATATAATAAAAATTTTAATTTGCTTTAATCGATAAAAAGATTATAAGTAGAATTTAAATTTGAAAATCAAACATTTGAATAAAGATTAGATTTATTTTTTAAAACAAATTAAAAAAAATGATAGAATGGAAGGATAACAGTAGATTAAGAACTTTATCTCTAATCAGAAAAATTATAATAATCTTATATTCAATTTTTAAAAAAAAAAAAAACAAAATCCATACAGCATTCGGAATTAGAATTTCATTGGAACTAAGAATTGTTATAAATTAAAAAAATAAATAGCGTAAAATAAGAATTCCTTTTTCCATTTTGAGAAGAAGAGAAGAGTTTTGCTCCATAAATAGAGATGAGGTGCATAAGCAAAAATACACCGATCGAGCTTTTTCCAAGTATCATATTTCGTAAGTTTTTCATTTTGCTTTTTTCCTAAACCCTAAGTTCCACATTGAATTTTTTTTATTTTTTATTTTTTCAGCTAATTCTCGACTGTGTTTAAGAAATCCTAAGTTTCTTCATTGGAGTACTACTGCGTCATCATCGTCGTCTGAACTGAAGGCCGCCGGCATGGCGTTTCCTTCCGGCGGCGTTGTGGGTTTTAAGTTCATGCCAACCGACGAAGAACTCGTAGCTCATTATCTTTACAGCAAAGTTCACGGGCTTGCCTACTACGAGGATGCCGTCAAAGATTTCGACCTCTACGGCAACGAGGAGCCATGGGTTGTTTTCGACATGTTCGGAGGTGTGGATGGGGAAGATATGTATTTCTTCACCCAGCTAAAGAGGAAGTGGCCTTCCAAACGAGGCTCTTCTCGGGTCAGCCGCCGGGTCGGTACCAATGGTGGAACGTGGAGCGCTGAGAATTCCGAAACCACAATTTTCTCTTCCCAGACCAATGGTCCCATCGGCTTCTGCAAACGCCTCCGCTACGAGAATGGTGGGTTGGAACACCATGGCGAGTGGATCATGCATGAGTATAGTTTGGATAAAGCTTACTGGCCCAACAATGGCGGCGGCGGCGGCGGCGGTGGCTGTGAGTATGTTTTGTGCCGGATTAGAAAGAACGACCGACCGATCAAGAAAGCGTTGAAGAGGAGATTCAGTGAGTTGGAGCTGCAACAATTACCAGCTTCATGTCGGCCTCCATCTCCATTTGCTGCTCACTTGGAGCAACAATTGTGGCATTAGAAGAGTCGGAACCAAATTACAATGAGATTATTGTTGAAGAAGAAGCCCCCGTGGCTGATTTTGAAAATGTGGAAGGATGCCACCCGCAAATGGACGAAAGTGATCCATTTGGCTTTGGTTTAGGCTCTACATCGTATCTTCAGATGCTTCGTGACACAGACGTATATTTTGCTGATGAAGAAAATGTGGACGCAAAGGCTACAAATTTTGAATA

mRNA sequence

ATGGATATTGCTGCAGAGCCATTTCGGAGAGGTTTTCGAGTCCTTTTGGTTATATTCTTCCTTTTGGTTTCTGGGTTTGGTTTGTCTGAAGGTTCCAAAACCAGACACTTCCAATGGGATGTAGAGTACATGTTCTGGTCTCCAGATTGCGTAGAGAATGTGGTTATGGGGATCAACGGCCAGTTTCCCGGTCCGACGATCAGAGCCAATGTCGGAGACACCGTCGTCGTCGATGTCACGAACAAGCTTTTCACCGAAGGCGTCGTCATTCACTGGCATGGAATCAGGCAGATCGGAACTCCGTGGTCTGATGGCACAGCTTCGATCTCGCAGTGTGCCATCAACCCGGGAGAAACCTTTGTTTATAGATTCAAAGTTGATAAGGCAGGAACGTACTTCTATCACGGGCATTATGGGATGCAAAGATCAGCAGGGTTGTATGGATCTTTGATAGTGGACGTGGCAGAAGGAGAGAAAGAGCCATTTCATTACGACCAAGAGATCAACTTGTTGCTTAGTGATTGGTGGCATCAAAGTGTTCATGAACAAGAGGTGGGCCTCAGCTCCAGTCCAACTCGATGGATCGGCAACCCTCAGAGTTTGTTGATAAATGGAAGAGGTCAGTTCAAGTGCTCAATTGCAGCTAAGTACAATATTAGCTTGAACCAATGCAAATTGAAGGGAGACGACCGACATCGACATTTATTCCGGCGAGAGCTACTCCGTCCTCATAACCACCAATCAAGACCCATCCAACAACTACTGGGTTTCCGCCGGCGTCAGAGGGCGAAAACCGGGGACCCCGCCGGCACTCACCCTCCTCAACTACCTCCCCAACTCCGCCTCCAAGTTGCCTGCTTCTCCGCCGCCGGTCACTCCCCGGTGGGACGACTTCGACCGCAGCAAGAACTTCTCCAAACAAATCTTCGCCGCCGTCGGCTCTGCGCAACCGCCAAGAATTTCAGCCGCCGGATCTTCCTTCTCAACACCCAGAACCAGATCAATGGTGTACAAAAGTGGGCTATGAACAACGTCTCCTTGGCGCTGCCGACGACGCCGTATCTCGGCGCCATCAAGTTCGGCTTAAACCAGTCTTTCGACCAAAGCAATCCGCCGGAGACTTTCCCACAGGATTACGACATCAACCGCCCTCCACCCAATCCCGCCACCACCATCGGAAACGCGATTTACCGCCTGGATCTGGGATCCACGGTGGACTTCATACTTCAGAACGCAAACACTTTGACCAAAAATTTGAGCGAGATCCACCCCTGGCATTTGCATGGCCACGACTTCTGGGTGTTGGGCTACGGAGAAGGAAAGTTCTCCGCCGCTGCCGACGAGAAGAAGCTCAACTTGAAGAACCCACCTCTGAAGAACACGGTGGTGATCTTCCCTTACGGATGGACGGCGGTGAGGTTCGTGGCGGACAACCCCGGCGTTTGGATCTTCCATTGCCATATCGAACCTCACCTGCATATGGGGATGGGTGTTATCTTCGCAGAAGGCGTCGATAAAGTCGGTGAGATTCCCAGGAGTGCTCTCTCGTGTGGGCTCACCGGACAAATAAATCCTAAGTTTCTTCATTGGAGTACTACTGCGTCATCATCGTCGTCTGAACTGAAGGCCGCCGGCATGGCGTTTCCTTCCGGCGGCGTTGTGGGTTTTAAGTTCATGCCAACCGACGAAGAACTCGTAGCTCATTATCTTTACAGCAAAGTTCACGGGCTTGCCTACTACGAGGATGCCGTCAAAGATTTCGACCTCTACGGCAACGAGGAGCCATGGGTTGTTTTCGACATGTTCGGAGGTGTGGATGGGGAAGATATGTATTTCTTCACCCAGCTAAAGAGGAAGTGGCCTTCCAAACGAGGCTCTTCTCGGGTCAGCCGCCGGGTCGGTACCAATGGTGGAACGTGGAGCGCTGAGAATTCCGAAACCACAATTTTCTCTTCCCAGACCAATGGTCCCATCGGCTTCTGCAAACGCCTCCGCTACGAGAATGGTGGGTTGGAACACCATGGCGAGTGGATCATGCATGAGTATAGTTTGGATAAAGCTTACTGGCCCAACAATGGCGGCGGCGGCGGCGGCGGTGGCTGTGAGTATGTTTTGTGCCGGATTAGAAAGAACGACCGACCGATCAAGAAAGCGTTGAAGAGGAGATTCAAAGAGTCGGAACCAAATTACAATGAGATTATTGTTGAAGAAGAAGCCCCCGTGGCTGATTTTGAAAATGTGGAAGGATGCCACCCGCAAATGGACGAAAGTGATCCATTTGGCTTTGGTTTAGGCTCTACATCGTATCTTCAGATGCTTCGTGACACAGACGTATATTTTGCTGATGAAGAAAATGTGGACGCAAAGGCTACAAATTTTGAATA

Coding sequence (CDS)

ATGGATATTGCTGCAGAGCCATTTCGGAGAGGTTTTCGAGTCCTTTTGGTTATATTCTTCCTTTTGGTTTCTGGGTTTGGTTTGTCTGAAGGTTCCAAAACCAGACACTTCCAATGGGATGTAGAGTACATGTTCTGGTCTCCAGATTGCGTAGAGAATGTGGTTATGGGGATCAACGGCCAGTTTCCCGGTCCGACGATCAGAGCCAATGTCGGAGACACCGTCGTCGTCGATGTCACGAACAAGCTTTTCACCGAAGGCGTCGTCATTCACTGGCATGGAATCAGGCAGATCGGAACTCCGTGGTCTGATGGCACAGCTTCGATCTCGCAGTGTGCCATCAACCCGGGAGAAACCTTTGTTTATAGATTCAAAGTTGATAAGGCAGGAACGTACTTCTATCACGGGCATTATGGGATGCAAAGATCAGCAGGGTTGTATGGATCTTTGATAGTGGACGTGGCAGAAGGAGAGAAAGAGCCATTTCATTACGACCAAGAGATCAACTTGTTGCTTAGTGATTGGTGGCATCAAAGTGTTCATGAACAAGAGGTGGGCCTCAGCTCCAGTCCAACTCGATGGATCGGCAACCCTCAGAGTTTGTTGATAAATGGAAGAGGTCAGTTCAAGTGCTCAATTGCAGCTAAGTACAATATTAGCTTGAACCAATGCAAATTGAAGGGAGACGACCGACATCGACATTTATTCCGGCGAGAGCTACTCCGTCCTCATAACCACCAATCAAGACCCATCCAACAACTACTGGGTTTCCGCCGGCGTCAGAGGGCGAAAACCGGGGACCCCGCCGGCACTCACCCTCCTCAACTACCTCCCCAACTCCGCCTCCAAGTTGCCTGCTTCTCCGCCGCCGGTCACTCCCCGGTGGGACGACTTCGACCGCAGCAAGAACTTCTCCAAACAAATCTTCGCCGCCGTCGGCTCTGCGCAACCGCCAAGAATTTCAGCCGCCGGATCTTCCTTCTCAACACCCAGAACCAGATCAATGGTGTACAAAAGTGGGCTATGAACAACGTCTCCTTGGCGCTGCCGACGACGCCGTATCTCGGCGCCATCAAGTTCGGCTTAAACCAGTCTTTCGACCAAAGCAATCCGCCGGAGACTTTCCCACAGGATTACGACATCAACCGCCCTCCACCCAATCCCGCCACCACCATCGGAAACGCGATTTACCGCCTGGATCTGGGATCCACGGTGGACTTCATACTTCAGAACGCAAACACTTTGACCAAAAATTTGAGCGAGATCCACCCCTGGCATTTGCATGGCCACGACTTCTGGGTGTTGGGCTACGGAGAAGGAAAGTTCTCCGCCGCTGCCGACGAGAAGAAGCTCAACTTGAAGAACCCACCTCTGAAGAACACGGTGGTGATCTTCCCTTACGGATGGACGGCGGTGAGGTTCGTGGCGGACAACCCCGGCGTTTGGATCTTCCATTGCCATATCGAACCTCACCTGCATATGGGGATGGGTGTTATCTTCGCAGAAGGCGTCGATAAAGTCGGTGAGATTCCCAGGAGTGCTCTCTCGTGTGGGCTCACCGGACAAATAAATCCTAAGTTTCTTCATTGGAGTACTACTGCGTCATCATCGTCGTCTGAACTGAAGGCCGCCGGCATGGCGTTTCCTTCCGGCGGCGTTGTGGGTTTTAAGTTCATGCCAACCGACGAAGAACTCGTAGCTCATTATCTTTACAGCAAAGTTCACGGGCTTGCCTACTACGAGGATGCCGTCAAAGATTTCGACCTCTACGGCAACGAGGAGCCATGGGTTGTTTTCGACATGTTCGGAGGTGTGGATGGGGAAGATATGTATTTCTTCACCCAGCTAAAGAGGAAGTGGCCTTCCAAACGAGGCTCTTCTCGGGTCAGCCGCCGGGTCGGTACCAATGGTGGAACGTGGAGCGCTGAGAATTCCGAAACCACAATTTTCTCTTCCCAGACCAATGGTCCCATCGGCTTCTGCAAACGCCTCCGCTACGAGAATGGTGGGTTGGAACACCATGGCGAGTGGATCATGCATGAGTATAGTTTGGATAAAGCTTACTGGCCCAACAATGGCGGCGGCGGCGGCGGCGGTGGCTGTGAGTATGTTTTGTGCCGGATTAGAAAGAACGACCGACCGATCAAGAAAGCGTTGAAGAGGAGATTCAAAGAGTCGGAACCAAATTACAATGAGATTATTGTTGAAGAAGAAGCCCCCGTGGCTGATTTTGAAAATGTGGAAGGATGCCACCCGCAAATGGACGAAAGTGATCCATTTGGCTTTGGTTTAGGCTCTACATCGTATCTTCAGATGCTTCGTGACACAGACGTATATTTTGCTGATGAAGAAAATGTGGACGCAAAGGCTACAAATTTTGAATA

Protein sequence

MDIAAEPFRRGFRVLLVIFFLLVSGFGLSEGSKTRHFQWDVEYMFWSPDCVENVVMGINGQFPGPTIRANVGDTVVVDVTNKLFTEGVVIHWHGIRQIGTPWSDGTASISQCAINPGETFVYRFKVDKAGTYFYHGHYGMQRSAGLYGSLIVDVAEGEKEPFHYDQEINLLLSDWWHQSVHEQEVGLSSSPTRWIGNPQSLLINGRGQFKCSIAAKYNISLNQCKLKGDDRHRHLFRRELLRPHNHQSRPIQQLLGFRRRQRAKTGDPAGTHPPQLPPQLRLQVACFSAAGHSPVGRLRPQQELLQTNLRRRRLCATAKNFSRRIFLLNTQNQINGVQKWAMNNVSLALPTTPYLGAIKFGLNQSFDQSNPPETFPQDYDINRPPPNPATTIGNAIYRLDLGSTVDFILQNANTLTKNLSEIHPWHLHGHDFWVLGYGEGKFSAAADEKKLNLKNPPLKNTVVIFPYGWTAVRFVADNPGVWIFHCHIEPHLHMGMGVIFAEGVDKVGEIPRSALSCGLTGQINPKFLHWSTTASSSSSELKAAGMAFPSGGVVGFKFMPTDEELVAHYLYSKVHGLAYYEDAVKDFDLYGNEEPWVVFDMFGGVDGEDMYFFTQLKRKWPSKRGSSRVSRRVGTNGGTWSAENSETTIFSSQTNGPIGFCKRLRYENGGLEHHGEWIMHEYSLDKAYWPNNGGGGGGGGCEYVLCRIRKNDRPIKKALKRRFKESEPNYNEIIVEEEAPVADFENVEGCHPQMDESDPFGFGLGSTSYLQMLRDTDVYFADEENVDAKATNFEX
Homology
BLAST of Sgr011741 vs. NCBI nr
Match: XP_038878978.1 (L-ascorbate oxidase [Benincasa hispida])

HSP 1 Score: 682.2 bits (1759), Expect = 5.5e-192
Identity = 347/564 (61.52%), Postives = 396/564 (70.21%), Query Frame = 0

Query: 17  VIFFLL--VSGFG--LSEGS--KTRHFQWDVEYMFWSPDCVENVVMGINGQFPGPTIRAN 76
           +IFF+L  + GFG  LSE    K RH++W+VEYMFWSPDCVEN+VMGI+GQFPGPTIRAN
Sbjct: 15  LIFFILSIIFGFGITLSEAGFPKIRHYKWEVEYMFWSPDCVENIVMGIDGQFPGPTIRAN 74

Query: 77  VGDTVVVDVTNKLFTEGVVIHWHGIRQIGTPWSDGTASISQCAINPGETFVYRFKVDKAG 136
           VGDTVVV++TNKL TEGVVIHWHGI Q GTPW+DGTASISQCAINPGETF YRF VDKAG
Sbjct: 75  VGDTVVVELTNKLHTEGVVIHWHGIMQRGTPWADGTASISQCAINPGETFTYRFVVDKAG 134

Query: 137 TYFYHGHYGMQRSAGLYGSLIVDVAEGEKEPFHYDQEINLLLSDWWHQSVHEQEVGLSSS 196
           TYFYHGH GMQRSAGLYGSLIVD  EG+ EPFHYD+EINLLLSDWWHQSVH+QEVGLSS 
Sbjct: 135 TYFYHGHLGMQRSAGLYGSLIVDPEEGKNEPFHYDEEINLLLSDWWHQSVHKQEVGLSSK 194

Query: 197 PTRWIGNPQSLLINGRGQFKCSIAAKYNISLNQCKLKGDDRHR----HLFRRELLRPHNH 256
           P RWIG PQ++LINGRGQF CSIAAKYN +L QC+LKG ++      H+   +  R    
Sbjct: 195 PIRWIGEPQTILINGRGQFDCSIAAKYNNNLKQCELKGGEQCAPFILHVLPNKTYRIRIA 254

Query: 257 QSRPIQQLLGFRRRQRAKTGDPAGTHPPQLPPQLRLQVACFSAAGHSP-VGRLRPQQELL 316
            +  +  L       R    +  G +   + P L   +  +S   +S  +   +P  E  
Sbjct: 255 STTALASLNFAIGNHRLLVVEADGNY---IQPFLTSDIDIYSGESYSVLITTDQPSSENY 314

Query: 317 QTNLR-RRRLCAT---------------------------------AKNF---------- 376
             ++  R RL  T                                 +KNF          
Sbjct: 315 WVSIGVRARLPKTPPGLTILNYLPNSVSKLPISPPPETPDWEDFDRSKNFTFRIFAAMGS 374

Query: 377 -------SRRIFLLNTQNQINGVQKWAMNNVSLALPTTPYLGAIKFGLNQSFDQSNPPET 436
                  +RRIFLLNTQN++NG  KWA+NNVSLALP TPYL A+KF LN +FDQ+ PPET
Sbjct: 375 PKPPMRYNRRIFLLNTQNRMNGFVKWAINNVSLALPPTPYLAAMKFKLNTAFDQNPPPET 434

Query: 437 FPQDYDINRPPPNPATTIGNAIYRLDLGSTVDFILQNANTLTKNLSEIHPWHLHGHDFWV 496
           FP DYDIN PP N  T  GN +YR ++G  VD ILQNAN L  N SEIHPWHLHGHDFWV
Sbjct: 435 FPIDYDINNPPQNANTITGNGVYRFNMGEIVDVILQNANMLNDNTSEIHPWHLHGHDFWV 494

Query: 497 LGYGEGKFSAAADEKKLNLKNPPLKNTVVIFPYGWTAVRFVADNPGVWIFHCHIEPHLHM 519
           LGYGEGKFSAA DEKKLNLKNPPL+NTVVIFPYGWTA+RFVADNPGVW FHCHIEPHLHM
Sbjct: 495 LGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHM 554

BLAST of Sgr011741 vs. NCBI nr
Match: XP_038880052.1 (L-ascorbate oxidase-like [Benincasa hispida])

HSP 1 Score: 682.2 bits (1759), Expect = 5.5e-192
Identity = 348/567 (61.38%), Postives = 402/567 (70.90%), Query Frame = 0

Query: 14  VLLVIFFLLVSGFG--LSEG-SKTRHFQWDVEYMFWSPDCVENVVMGINGQFPGPTIRAN 73
           +++ + F ++SGFG  LSE  + TR+F+W+VEYMFWSPDCVEN+VMGINGQFPGPTIRAN
Sbjct: 15  LVIFLIFSMISGFGITLSEAVNNTRYFKWEVEYMFWSPDCVENLVMGINGQFPGPTIRAN 74

Query: 74  VGDTVVVDVTNKLFTEGVVIHWHGIRQIGTPWSDGTASISQCAINPGETFVYRFKVDKAG 133
           VGDT++V++ NKLFTEGVVIHWHGI Q  TPW+DGTASISQCAINPGETF+Y+FK DK G
Sbjct: 75  VGDTIIVELVNKLFTEGVVIHWHGILQRETPWADGTASISQCAINPGETFIYQFKADKPG 134

Query: 134 TYFYHGHYGMQRSAGLYGSLIVDVAEGEKEPFHYDQEINLLLSDWWHQSVHEQEVGLSSS 193
           TYFYHGH GMQRSAGLYGSLI+D  EG+KEPFHYD+EINLLLSDWWH+S H+QEVGLSSS
Sbjct: 135 TYFYHGHLGMQRSAGLYGSLIIDPEEGKKEPFHYDEEINLLLSDWWHKSAHKQEVGLSSS 194

Query: 194 PTRWIGNPQSLLINGRGQFKCSIAAKYNISLNQCKLKGDD---------RHRHLFRREL- 253
           P RWIG P+++LINGRGQ  CSIAAKYN +L QCKLKG +         + +  +R  + 
Sbjct: 195 PMRWIGEPETILINGRGQISCSIAAKYNKNLEQCKLKGGEQCAPFILQVKPKKTYRIRIA 254

Query: 254 ---------LRPHNHQ---------------SRPIQQLLGFRRRQRAKTG-DPAGTH--- 313
                    L   NHQ               +  I    G        T  DPA  +   
Sbjct: 255 STTSLSALNLAIGNHQLLVVEADGNYVEPFVTSDIDIYSGESYSVLITTNQDPAQNYWVS 314

Query: 314 ------PPQLPPQLRLQVACFSAAGHSPVG--RLRPQQE--LLQTNLRRRRLCATAK--- 373
                  P+ PP L L     ++    PV      P  E      N   R L A      
Sbjct: 315 VGVRGRLPKTPPALTLLNYLPNSVAKLPVSPPPETPNWEDYARSKNFTYRILAAMGSPNP 374

Query: 374 --NFSRRIFLLNTQNQIN-GVQKWAMNNVSLALPTTPYLGAIKFGLNQSFDQSNPPETFP 433
              F+RRIFLLNTQN++N G  KWA+NNVSL+LP+TPYLGAIK GLN +F+Q+ PPETF 
Sbjct: 375 PIKFNRRIFLLNTQNRMNGGFTKWAINNVSLSLPSTPYLGAIKIGLNNTFNQNPPPETFS 434

Query: 434 QDYDINRPPPNPATTIGNAIYRLDLGSTVDFILQNANTLTKNLSEIHPWHLHGHDFWVLG 493
           +DYDI  PPPNP T   N +Y  +LG TVD ILQNANTL    SEIHPWHLHGHDFWVLG
Sbjct: 435 EDYDIFNPPPNPNTITDNGVYMFNLGETVDVILQNANTLNDKHSEIHPWHLHGHDFWVLG 494

Query: 494 YGEGKFSAAADEKKLNLKNPPLKNTVVIFPYGWTAVRFVADNPGVWIFHCHIEPHLHMGM 524
           YGEGKFSAA DEKKLNLKNPPL+NTVVIFPYGWTA+RFVADNPGVW FHCHIEPHLHMGM
Sbjct: 495 YGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMGM 554

BLAST of Sgr011741 vs. NCBI nr
Match: CBY84386.1 (ascorbate oxidase [Cucumis sativus])

HSP 1 Score: 676.0 bits (1743), Expect = 3.9e-190
Identity = 343/580 (59.14%), Postives = 394/580 (67.93%), Query Frame = 0

Query: 3   IAAEP-FRRGFRVLLVIFFLLVSGFGLSEGS--KTRHFQWDVEYMFWSPDCVENVVMGIN 62
           +A +P F + F   LV+  +   G  LSE    K +H++WDVEYMFWSPDCVEN+VMGIN
Sbjct: 4   VADKPFFPKPFLSFLVLSIIFGFGITLSEAGFPKIKHYKWDVEYMFWSPDCVENIVMGIN 63

Query: 63  GQFPGPTIRANVGDTVVVDVTNKLFTEGVVIHWHGIRQIGTPWSDGTASISQCAINPGET 122
           G+FPGPTIRAN GD VVV++TNKL TEGVVIHWHGI Q GTPW+DGTASISQCAINPGET
Sbjct: 64  GEFPGPTIRANAGDIVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGET 123

Query: 123 FVYRFKVDKAGTYFYHGHYGMQRSAGLYGSLIVDVAEGEKEPFHYDQEINLLLSDWWHQS 182
           F YRF VDKAGTYFYHGH GMQRSAGLYGSLIVD  EG  EPFHYD+EINLLLSDWWHQS
Sbjct: 124 FTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPPEGRSEPFHYDEEINLLLSDWWHQS 183

Query: 183 VHEQEVGLSSSPTRWIGNPQSLLINGRGQFKCSIAAKYNISLNQCKLKGDDRHR----HL 242
           VH+QEVGLSS P RWIG PQS+LING+GQF CSIAAKYN  L QC+L G ++      H+
Sbjct: 184 VHKQEVGLSSKPMRWIGEPQSILINGKGQFDCSIAAKYNQGLKQCELSGKEKCAPFILHV 243

Query: 243 FRRELLRPH---------------NHQSRPIQQ-----------------------LLGF 302
             ++  R                 NH+   ++                        L+  
Sbjct: 244 QPKKTYRIRIASTTALASLNFAIGNHELLVVEADGNYVQPFVTSDIDIYSGESYSVLITT 303

Query: 303 RRRQRAKTGDPAGTHP--PQLPPQLRLQVACFSAAGHSPVGRLRPQQEL-------LQTN 362
            +          G     P+ PP L L     ++A   P+    P  E           N
Sbjct: 304 DQNPLENYWVSIGVRARLPKTPPGLTLLNYLPNSASKLPIS---PPPETPHWEDFDRSKN 363

Query: 363 LRRRRLCATAK-----NFSRRIFLLNTQNQINGVQKWAMNNVSLALPTTPYLGAIKFGLN 422
              R   A         ++RR+FLLNTQN+ING  KWA+NNVSLALP TPYL A+K  LN
Sbjct: 364 FTFRIFAAMGSPKPPVRYNRRLFLLNTQNRINGFMKWAINNVSLALPPTPYLAAMKMRLN 423

Query: 423 QSFDQSNPPETFPQDYDINRPPPNPATTIGNAIYRLDLGSTVDFILQNANTLTKNLSEIH 482
            +F+QS PPETFP +YDIN PPPNP TT GN +Y+ ++G TVD ILQNAN L  N+SEIH
Sbjct: 424 TAFNQSPPPETFPLNYDINNPPPNPETTTGNGVYKFNMGETVDVILQNANMLNPNMSEIH 483

Query: 483 PWHLHGHDFWVLGYGEGKFSAAADEKKLNLKNPPLKNTVVIFPYGWTAVRFVADNPGVWI 524
           PWHLHGHDFWVLGYGEGKF A  DEKKLNLKNPPL+NTVVIFPYGWTA+RFVADNPGVW 
Sbjct: 484 PWHLHGHDFWVLGYGEGKFYAPEDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWA 543

BLAST of Sgr011741 vs. NCBI nr
Match: XP_004149015.1 (L-ascorbate oxidase [Cucumis sativus] >P14133.1 RecName: Full=L-ascorbate oxidase; Short=ASO; Short=Ascorbase; Flags: Precursor [Cucumis sativus] >AAA33119.1 ascorbate oxidase precursor (EC 1.10.3.3) [Cucumis sativus] >KGN66418.1 hypothetical protein Csa_007350 [Cucumis sativus])

HSP 1 Score: 674.9 bits (1740), Expect = 8.7e-190
Identity = 342/580 (58.97%), Postives = 394/580 (67.93%), Query Frame = 0

Query: 3   IAAEP-FRRGFRVLLVIFFLLVSGFGLSEGS--KTRHFQWDVEYMFWSPDCVENVVMGIN 62
           +A +P F + F   LV+  +   G  LSE    K +H++WDVEYMFWSPDCVEN+VMGIN
Sbjct: 4   VADKPFFPKPFLSFLVLSIIFGFGITLSEAGFPKIKHYKWDVEYMFWSPDCVENIVMGIN 63

Query: 63  GQFPGPTIRANVGDTVVVDVTNKLFTEGVVIHWHGIRQIGTPWSDGTASISQCAINPGET 122
           G+FPGPTIRAN GD VVV++TNKL TEGVVIHWHGI Q GTPW+DGTASISQCAINPGET
Sbjct: 64  GEFPGPTIRANAGDIVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGET 123

Query: 123 FVYRFKVDKAGTYFYHGHYGMQRSAGLYGSLIVDVAEGEKEPFHYDQEINLLLSDWWHQS 182
           F YRF VDKAGTYFYHGH GMQRSAGLYGSLIVD  EG  EPFHYD+EINLLLSDWWHQS
Sbjct: 124 FTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPPEGRSEPFHYDEEINLLLSDWWHQS 183

Query: 183 VHEQEVGLSSSPTRWIGNPQSLLINGRGQFKCSIAAKYNISLNQCKLKGDDRHR----HL 242
           VH+QEVGLSS P RWIG PQS+LING+GQF CSIAAKYN  L QC+L G ++      H+
Sbjct: 184 VHKQEVGLSSKPMRWIGEPQSILINGKGQFDCSIAAKYNQGLKQCELSGKEKCAPFILHV 243

Query: 243 FRRELLRPH---------------NHQSRPIQQ-----------------------LLGF 302
             ++  R                 NH+   ++                        L+  
Sbjct: 244 QPKKTYRIRIASTTALASLNFAIGNHELLVVEADGNYVQPFVTSDIDIYSGESYSVLITT 303

Query: 303 RRRQRAKTGDPAGTHP--PQLPPQLRLQVACFSAAGHSPVGRLRPQQEL-------LQTN 362
            +          G     P+ PP L L     ++A   P+    P  E           N
Sbjct: 304 DQNPLENYWVSIGVRARLPKTPPGLTLLNYLPNSASKLPIS---PPPETPHWEDFDRSKN 363

Query: 363 LRRRRLCATAK-----NFSRRIFLLNTQNQINGVQKWAMNNVSLALPTTPYLGAIKFGLN 422
              R   A         ++RR+FLLNTQN+ING  KWA+NNVSLALP TPYL A+K  LN
Sbjct: 364 FTFRIFAAMGSPKPPVRYNRRLFLLNTQNRINGFMKWAINNVSLALPPTPYLAAMKMRLN 423

Query: 423 QSFDQSNPPETFPQDYDINRPPPNPATTIGNAIYRLDLGSTVDFILQNANTLTKNLSEIH 482
            +F+Q+ PPETFP +YDIN PPPNP TT GN +Y+ ++G TVD ILQNAN L  N+SEIH
Sbjct: 424 TAFNQNPPPETFPLNYDINNPPPNPETTTGNGVYKFNMGETVDVILQNANMLNPNMSEIH 483

Query: 483 PWHLHGHDFWVLGYGEGKFSAAADEKKLNLKNPPLKNTVVIFPYGWTAVRFVADNPGVWI 524
           PWHLHGHDFWVLGYGEGKF A  DEKKLNLKNPPL+NTVVIFPYGWTA+RFVADNPGVW 
Sbjct: 484 PWHLHGHDFWVLGYGEGKFYAPEDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWA 543

BLAST of Sgr011741 vs. NCBI nr
Match: NP_001284441.1 (L-ascorbate oxidase [Cucumis melo] >AAF35911.2 ascorbate oxidase AO4 [Cucumis melo])

HSP 1 Score: 674.1 bits (1738), Expect = 1.5e-189
Identity = 344/580 (59.31%), Postives = 397/580 (68.45%), Query Frame = 0

Query: 3   IAAEP-FRRGFRVLLVIFFLLVSGFGLSEGS--KTRHFQWDVEYMFWSPDCVENVVMGIN 62
           +A +P F + F V  V+  +   G  LSE    K RH++W+VEYMFWSPDCVEN+VMGIN
Sbjct: 4   VADKPFFPKPFLVFFVLSIIFGFGITLSEAGFPKIRHYKWEVEYMFWSPDCVENIVMGIN 63

Query: 63  GQFPGPTIRANVGDTVVVDVTNKLFTEGVVIHWHGIRQIGTPWSDGTASISQCAINPGET 122
           GQFPGPTIRAN GD VVV++TNKL TEGVVIHWHGI Q GTPW+DGTASISQCAINPGET
Sbjct: 64  GQFPGPTIRANAGDMVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGET 123

Query: 123 FVYRFKVDKAGTYFYHGHYGMQRSAGLYGSLIVDVAEGEKEPFHYDQEINLLLSDWWHQS 182
           F YRF VDKAGTYFYHGH GMQRSAGLYGSLIVD  EG  EPFHYD+EINLLLSDWWHQS
Sbjct: 124 FTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPQEGTSEPFHYDEEINLLLSDWWHQS 183

Query: 183 VHEQEVGLSSSPTRWIGNPQSLLINGRGQFKCSIAAKYNISLNQCKLKGDDRHR----HL 242
           VH+QEVGLSS P RWIG PQS+LINGRGQF CSIAAKY   L QC+L G ++      H+
Sbjct: 184 VHKQEVGLSSKPMRWIGEPQSILINGRGQFDCSIAAKYKNGLKQCELSGKEQCAPFILHV 243

Query: 243 FRRELLRPHNHQSRPIQQL---LGFRRR----------QRAKTGD--------------- 302
             +++ R     +  +  L   +G  +           Q   T D               
Sbjct: 244 HPKKIYRIRIASTTALASLNFAIGNHKLLVVEADGNYVQPFSTSDIDIYSGESYSVLITT 303

Query: 303 ---PAGTH---------PPQLPPQLRLQVACFSAAGHSPVGRLRPQQEL-------LQTN 362
              P+  +          P+ PP L +     ++    P+    P  E           N
Sbjct: 304 DQNPSENYWVSIGVRARLPKTPPGLTVLNYLPNSVSKLPIS---PPPETPDWEDFDRSKN 363

Query: 363 LRRRRLCATAK-----NFSRRIFLLNTQNQINGVQKWAMNNVSLALPTTPYLGAIKFGLN 422
              R   A         ++RR+FLLNTQN+ING  KWA+NNVSLALP TPYL A+KF L+
Sbjct: 364 FTFRIFAAMGSPKPPVRYNRRLFLLNTQNRINGFMKWAINNVSLALPPTPYLAAMKFRLS 423

Query: 423 QSFDQSNPPETFPQDYDINRPPPNPATTIGNAIYRLDLGSTVDFILQNANTLTKNLSEIH 482
            +F+Q+ PPETFP DYDIN PP NP T  GN +Y+ ++G TVD ILQNAN L  N SEIH
Sbjct: 424 TAFNQNPPPETFPIDYDINNPPRNPETATGNGVYKFNMGETVDVILQNANMLNANTSEIH 483

Query: 483 PWHLHGHDFWVLGYGEGKFSAAADEKKLNLKNPPLKNTVVIFPYGWTAVRFVADNPGVWI 524
           PWHLHGHDFWVLGYGEGKFSAA D +KLNLKNPPL+NTVVIFPYGWTA+RFVADNPGVW 
Sbjct: 484 PWHLHGHDFWVLGYGEGKFSAAEDGRKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWA 543

BLAST of Sgr011741 vs. ExPASy Swiss-Prot
Match: P14133 (L-ascorbate oxidase OS=Cucumis sativus OX=3659 PE=1 SV=1)

HSP 1 Score: 674.9 bits (1740), Expect = 1.1e-192
Identity = 342/580 (58.97%), Postives = 394/580 (67.93%), Query Frame = 0

Query: 3   IAAEP-FRRGFRVLLVIFFLLVSGFGLSEGS--KTRHFQWDVEYMFWSPDCVENVVMGIN 62
           +A +P F + F   LV+  +   G  LSE    K +H++WDVEYMFWSPDCVEN+VMGIN
Sbjct: 4   VADKPFFPKPFLSFLVLSIIFGFGITLSEAGFPKIKHYKWDVEYMFWSPDCVENIVMGIN 63

Query: 63  GQFPGPTIRANVGDTVVVDVTNKLFTEGVVIHWHGIRQIGTPWSDGTASISQCAINPGET 122
           G+FPGPTIRAN GD VVV++TNKL TEGVVIHWHGI Q GTPW+DGTASISQCAINPGET
Sbjct: 64  GEFPGPTIRANAGDIVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGET 123

Query: 123 FVYRFKVDKAGTYFYHGHYGMQRSAGLYGSLIVDVAEGEKEPFHYDQEINLLLSDWWHQS 182
           F YRF VDKAGTYFYHGH GMQRSAGLYGSLIVD  EG  EPFHYD+EINLLLSDWWHQS
Sbjct: 124 FTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPPEGRSEPFHYDEEINLLLSDWWHQS 183

Query: 183 VHEQEVGLSSSPTRWIGNPQSLLINGRGQFKCSIAAKYNISLNQCKLKGDDRHR----HL 242
           VH+QEVGLSS P RWIG PQS+LING+GQF CSIAAKYN  L QC+L G ++      H+
Sbjct: 184 VHKQEVGLSSKPMRWIGEPQSILINGKGQFDCSIAAKYNQGLKQCELSGKEKCAPFILHV 243

Query: 243 FRRELLRPH---------------NHQSRPIQQ-----------------------LLGF 302
             ++  R                 NH+   ++                        L+  
Sbjct: 244 QPKKTYRIRIASTTALASLNFAIGNHELLVVEADGNYVQPFVTSDIDIYSGESYSVLITT 303

Query: 303 RRRQRAKTGDPAGTHP--PQLPPQLRLQVACFSAAGHSPVGRLRPQQEL-------LQTN 362
            +          G     P+ PP L L     ++A   P+    P  E           N
Sbjct: 304 DQNPLENYWVSIGVRARLPKTPPGLTLLNYLPNSASKLPIS---PPPETPHWEDFDRSKN 363

Query: 363 LRRRRLCATAK-----NFSRRIFLLNTQNQINGVQKWAMNNVSLALPTTPYLGAIKFGLN 422
              R   A         ++RR+FLLNTQN+ING  KWA+NNVSLALP TPYL A+K  LN
Sbjct: 364 FTFRIFAAMGSPKPPVRYNRRLFLLNTQNRINGFMKWAINNVSLALPPTPYLAAMKMRLN 423

Query: 423 QSFDQSNPPETFPQDYDINRPPPNPATTIGNAIYRLDLGSTVDFILQNANTLTKNLSEIH 482
            +F+Q+ PPETFP +YDIN PPPNP TT GN +Y+ ++G TVD ILQNAN L  N+SEIH
Sbjct: 424 TAFNQNPPPETFPLNYDINNPPPNPETTTGNGVYKFNMGETVDVILQNANMLNPNMSEIH 483

Query: 483 PWHLHGHDFWVLGYGEGKFSAAADEKKLNLKNPPLKNTVVIFPYGWTAVRFVADNPGVWI 524
           PWHLHGHDFWVLGYGEGKF A  DEKKLNLKNPPL+NTVVIFPYGWTA+RFVADNPGVW 
Sbjct: 484 PWHLHGHDFWVLGYGEGKFYAPEDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWA 543

BLAST of Sgr011741 vs. ExPASy Swiss-Prot
Match: P24792 (L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=AAO PE=1 SV=2)

HSP 1 Score: 651.4 bits (1679), Expect = 1.4e-185
Identity = 329/565 (58.23%), Postives = 384/565 (67.96%), Query Frame = 0

Query: 15  LLVIFFLLVSGFGL-SEGSKTRHFQWDVEYMFWSPDCVENVVMGINGQFPGPTIRANVGD 74
           L++ FF L+  FG+ SEGS+ RH++W+VEYMFW+PDC EN+VMGINGQFPGPTIRAN GD
Sbjct: 13  LILFFFGLILAFGISSEGSQIRHYKWEVEYMFWAPDCNENIVMGINGQFPGPTIRANAGD 72

Query: 75  TVVVDVTNKLFTEGVVIHWHGIRQIGTPWSDGTASISQCAINPGETFVYRFKVDKAGTYF 134
           TVVV++ NKL TEGVVIHWHGI Q GTPW+DGTASISQCAINPGETF Y F VD  GT+F
Sbjct: 73  TVVVELINKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETFFYNFTVDNPGTFF 132

Query: 135 YHGHYGMQRSAGLYGSLIVDVAEGEKEPFHYDQEINLLLSDWWHQSVHEQEVGLSSSPTR 194
           YHGH GMQRSAGLYGSLIVD  +G+KEPFHYD EINLLLSDWWHQS+H+QEVGLSS P R
Sbjct: 133 YHGHLGMQRSAGLYGSLIVDPPQGKKEPFHYDGEINLLLSDWWHQSIHKQEVGLSSKPIR 192

Query: 195 WIGNPQSLLINGRGQFKCSIAAKYNISLNQCKLKGDDRHR----HLFRRELLRPH----- 254
           WIG PQ++L+NGRGQF CSIAAKY+ +L  CKLKG +       H+  ++  R       
Sbjct: 193 WIGEPQTILLNGRGQFDCSIAAKYDSNLEPCKLKGSEPCAPYIFHVMPKKTYRIRIASTT 252

Query: 255 ----------NHQSRPIQQ-----------------------LLGFRRRQRAKTGDPAGT 314
                     NH    ++                        L+   +          GT
Sbjct: 253 ALAALNFAIGNHPLLVVEADGNYVQPFYTSDIDIYSGESYSVLITTDQNPSENYWVSVGT 312

Query: 315 --HPPQLPPQLRLQVACFSAAGHSPVGRLRPQQEL-------LQTNLRRRRLCATAK--- 374
               P  PP L L     ++    P     P  E           N   R   A      
Sbjct: 313 RGRHPNTPPGLTLLNYLPNSVSKLPTS---PPPETPAWDDFDRSKNFTYRITAAMGSPKP 372

Query: 375 --NFSRRIFLLNTQNQINGVQKWAMNNVSLALPTTPYLGAIKFGLNQSFDQSNPPETFPQ 434
               +RRIFLLNTQN ING  KWA+N+VSLALP TPYLGA+KF L  +FDQ+ PPE FP+
Sbjct: 373 PVKSNRRIFLLNTQNVINGYVKWAINDVSLALPPTPYLGAMKFNLLHAFDQNPPPEVFPE 432

Query: 435 DYDINRPPPNPATTIGNAIYRLDLGSTVDFILQNANTLTKNLSEIHPWHLHGHDFWVLGY 494
           DYDI+ PP N  T IGN +Y+  +G  VD ILQNAN + +NLSEIHPWHLHGHDFWVLGY
Sbjct: 433 DYDIDTPPTNEKTKIGNGVYQFKIGEIVDVILQNANMMKENLSEIHPWHLHGHDFWVLGY 492

Query: 495 GEGKFSAAADEKKLNLKNPPLKNTVVIFPYGWTAVRFVADNPGVWIFHCHIEPHLHMGMG 523
           G+GKF+ A +E  LNLKNPPL+NTVVIFPYGWTA+RFVADNPGVW FHCHIEPHLHMGMG
Sbjct: 493 GDGKFT-AEEESSLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMGMG 552

BLAST of Sgr011741 vs. ExPASy Swiss-Prot
Match: P37064 (L-ascorbate oxidase OS=Cucurbita pepo var. melopepo OX=3665 PE=1 SV=1)

HSP 1 Score: 641.0 bits (1652), Expect = 1.8e-182
Identity = 322/551 (58.44%), Postives = 379/551 (68.78%), Query Frame = 0

Query: 32  SKTRHFQWDVEYMFWSPDCVENVVMGINGQFPGPTIRANVGDTVVVDVTNKLFTEGVVIH 91
           S+ RH++W+VEYMFW+P+C EN+VMGINGQFPGPTIRAN GD+VVV++TNKL TEGVVIH
Sbjct: 1   SQIRHYKWEVEYMFWAPNCNENIVMGINGQFPGPTIRANAGDSVVVELTNKLHTEGVVIH 60

Query: 92  WHGIRQIGTPWSDGTASISQCAINPGETFVYRFKVDKAGTYFYHGHYGMQRSAGLYGSLI 151
           WHGI Q GTPW+DGTASISQCAINPGETF Y F VD  GT+FYHGH GMQRSAGLYGSLI
Sbjct: 61  WHGILQRGTPWADGTASISQCAINPGETFFYNFTVDNPGTFFYHGHLGMQRSAGLYGSLI 120

Query: 152 VDVAEGEKEPFHYDQEINLLLSDWWHQSVHEQEVGLSSSPTRWIGNPQSLLINGRGQFKC 211
           VD  +G+KEPFHYD EINLLLSDWWHQS+H+QEVGLSS P RWIG PQ++L+NGRGQF C
Sbjct: 121 VDPPQGKKEPFHYDGEINLLLSDWWHQSIHKQEVGLSSKPIRWIGEPQTILLNGRGQFDC 180

Query: 212 SIAAKYNISLNQCKLKGDDRHR----HLFRRELLRPH---------------NHQSRPIQ 271
           SIAAKY+ +L  CKLKG +       H+  ++  R                 NHQ   ++
Sbjct: 181 SIAAKYDSNLEPCKLKGSESCAPYIFHVSPKKTYRIRIASTTALAALNFAIGNHQLLVVE 240

Query: 272 Q-----------------------LLGFRRRQRAKTGDPAGTHP--PQLPPQLRL---QV 331
                                   L+   +          GT    P  PP L L     
Sbjct: 241 ADGNYVQPFYTSDIDIYSGESYSVLITTDQNPSENYWVSVGTRARHPNTPPGLTLLNYLP 300

Query: 332 ACFSAAGHSPVGRLRPQQELLQTNLRRRRLCATAKN------FSRRIFLLNTQNQINGVQ 391
              S    SP  +     +  ++     R+ A   +      F+RRIFLLNTQN ING  
Sbjct: 301 NSVSKLPTSPPPQTPAWDDFDRSKNFTYRITAAMGSPKPPVKFNRRIFLLNTQNVINGYV 360

Query: 392 KWAMNNVSLALPTTPYLGAIKFGLNQSFDQSNPPETFPQDYDINRPPPNPATTIGNAIYR 451
           KWA+N+VSLALP TPYLGA+K+ L  +FDQ+ PPE FP+DYDI+ PP N  T IGN +Y+
Sbjct: 361 KWAINDVSLALPPTPYLGAMKYNLLHAFDQNPPPEVFPEDYDIDTPPTNEKTRIGNGVYQ 420

Query: 452 LDLGSTVDFILQNANTLTKNLSEIHPWHLHGHDFWVLGYGEGKFSAAADEKKLNLKNPPL 511
             +G  VD ILQNAN + +NLSE HPWHLHGHDFWVLGYG+GKFS A +E  LNLKNPPL
Sbjct: 421 FKIGEVVDVILQNANMMKENLSETHPWHLHGHDFWVLGYGDGKFS-AEEESSLNLKNPPL 480

Query: 512 KNTVVIFPYGWTAVRFVADNPGVWIFHCHIEPHLHMGMGVIFAEGVDKVGEIPRSALSCG 527
           +NTVVIFPYGWTA+RFVADNPGVW FHCHIEPHLHMGMGV+FAEGV+KVG IP  AL+CG
Sbjct: 481 RNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGVEKVGRIPTKALACG 540

BLAST of Sgr011741 vs. ExPASy Swiss-Prot
Match: Q40588 (L-ascorbate oxidase OS=Nicotiana tabacum OX=4097 GN=AAO PE=2 SV=1)

HSP 1 Score: 618.6 bits (1594), Expect = 9.7e-176
Identity = 319/570 (55.96%), Postives = 379/570 (66.49%), Query Frame = 0

Query: 12  FRVLLVIFFLLVSGFGLSEGSKTRHFQWDVEYMFWSPDCVENVVMGINGQFPGPTIRANV 71
           F  LL +  L +S       +KTRHF+WDVEY+ WSPD  E+VVMGINGQFPGPTIRA  
Sbjct: 8   FFFLLPLILLELSSSRSVMAAKTRHFKWDVEYIHWSPDGEESVVMGINGQFPGPTIRAKA 67

Query: 72  GDTVVVDVTNKLFTEGVVIHWHGIRQIGTPWSDGTASISQCAINPGETFVYRFKVDKAGT 131
           GDTV V +TNKL TEGVVIHWHGIRQIGTPW+DGTA+ISQCAINPGETF+YRFKVDKAGT
Sbjct: 68  GDTVAVHLTNKLHTEGVVIHWHGIRQIGTPWADGTAAISQCAINPGETFLYRFKVDKAGT 127

Query: 132 YFYHGHYGMQRSAGLYGSLIVDVAEGEKEPFHYDQEINLLLSDWWHQSVHEQEVGLSSSP 191
           YFYHGHYGMQRSAGLYGSLIV+V EGEKEPFHYD E NLLLSDWWH+  HEQEV LSS+P
Sbjct: 128 YFYHGHYGMQRSAGLYGSLIVEVGEGEKEPFHYDGEFNLLLSDWWHKGSHEQEVDLSSNP 187

Query: 192 TRWIGNPQSLLINGRGQFKCSIAAKYN-ISLNQCKLKGDDRHRHLFRRELLRPHNHQSRP 251
            RWIG PQ+LL+NGRGQ+ CS+AA+++   L QCKL+G +++     R  +RP+      
Sbjct: 188 LRWIGEPQTLLLNGRGQYNCSLAARFSKPPLPQCKLRGGEQYAPQILR--VRPNKIYRLR 247

Query: 252 IQQLLGFRRRQRAKTG-------------------------------------DPAGTH- 311
           +           A  G                                     DP   + 
Sbjct: 248 VASTTALGSLSLAIGGHKMVVVEADGNYVQPFSVQDMDIYSGESYSVLFKTDQDPTKNYW 307

Query: 312 --------PPQLPPQLRLQVACFSAAGHSPVGRLRPQQELLQTNLRRRR--------LCA 371
                    P+ P  L L     ++A   P   L P    L  +    +        L  
Sbjct: 308 ISINVRGREPKTPQGLTLLNYLPNSASKFPT--LPPPIAPLWNDYNHSKSFSNKIFALMG 367

Query: 372 TAK---NFSRRIFLLNTQNQINGVQKWAMNNVSLALPTTPYLGAIKFGLNQSFDQSNPPE 431
           + K      RRI LLNTQN+I+G  KWA+NNVSL LPT  YLG+I++G+N +FD   PP+
Sbjct: 368 SPKPPPQNHRRIILLNTQNKIDGYTKWAINNVSLVLPTQLYLGSIRYGIN-AFDTKPPPD 427

Query: 432 TFPQDYDINRPPPNPATTIGNAIYRLDLGSTVDFILQNANTLTKNLSEIHPWHLHGHDFW 491
            FP+DYD+ +  PN  +T GN +Y L   +T+D ILQNAN L K++SEIHPWHLHGHDFW
Sbjct: 428 NFPKDYDVLKQAPNSNSTYGNGVYMLKFNTTIDIILQNANALAKDVSEIHPWHLHGHDFW 487

Query: 492 VLGYGEGKFSAAADEKKLNLKNPPLKNTVVIFPYGWTAVRFVADNPGVWIFHCHIEPHLH 524
           VLGYGEGKFS   D KK NLKNPPL+NT VIFP+GWTA+RFV DNPGVW FHCHIEPHLH
Sbjct: 488 VLGYGEGKFS-EKDVKKFNLKNPPLRNTAVIFPFGWTALRFVTDNPGVWAFHCHIEPHLH 547

BLAST of Sgr011741 vs. ExPASy Swiss-Prot
Match: M4DUF2 (L-ascorbate oxidase OS=Brassica rapa subsp. pekinensis OX=51351 GN=AO PE=2 SV=1)

HSP 1 Score: 563.1 bits (1450), Expect = 4.8e-159
Identity = 283/550 (51.45%), Postives = 343/550 (62.36%), Query Frame = 0

Query: 35  RHFQWDVEYMFWSPDCVENVVMGINGQFPGPTIRANVGDTVVVDVTNKLFTEGVVIHWHG 94
           R + W+VEY F  PDC E +VM +NGQFPGPTI A  GDT+VV +TNKL TEG+VIHWHG
Sbjct: 21  REYAWEVEYKFGWPDCKEGMVMAVNGQFPGPTIHALAGDTIVVHLTNKLATEGLVIHWHG 80

Query: 95  IRQIGTPWSDGTASISQCAINPGETFVYRFKVDKAGTYFYHGHYGMQRSAGLYGSLIVDV 154
           IRQ+G+PW+DG A ++QCAI+PGETF Y F VDK GT+FYHGHYGMQRSAGLYGSLI+DV
Sbjct: 81  IRQLGSPWADGAAGVTQCAISPGETFTYNFTVDKPGTHFYHGHYGMQRSAGLYGSLIIDV 140

Query: 155 AEGEKEPFHYDQEINLLLSDWWHQSVHEQEVGLSSSPTRWIGNPQSLLINGRGQFKCSIA 214
           A+G+KEP  YD E NLLLSDWWH+ V  QE+GLSS P RWIG  QS+LINGRGQF CS+A
Sbjct: 141 AKGKKEPLRYDGEFNLLLSDWWHEDVLSQEIGLSSRPMRWIGEAQSILINGRGQFNCSLA 200

Query: 215 AKY-NISLNQCKLKGDD----RHRHLFRRELLRPHNHQSRPIQQLLGFRRRQRAKTGDPA 274
           A++ + SL  C  K  D    +  H+   +  R     S  +  L    +  +    +  
Sbjct: 201 AQFSSTSLPTCTFKEGDQCAPQRLHVEPNKTYRIRLASSTALASLNFAVQGHKLVVVEAD 260

Query: 275 GTH----------------------------------------PPQLPPQLRLQVACFSA 334
           G +                                         P  PP L +     + 
Sbjct: 261 GNYITPFTTDDIDIYSGETYSVLLTTDQDPSQNYYITAGVRGRKPNTPPALTVLNYVTAP 320

Query: 335 AGHSPVGRLRPQQELLQTNLRRRRLCATAKNFSRRIF-----------------LLNTQN 394
           +   P     P  E  + N   R     +KNFS++IF                 LLNTQN
Sbjct: 321 SSQLPTS---PPPETPRWNDFDR-----SKNFSKKIFAAMGSPSPPETFDERLILLNTQN 380

Query: 395 QINGVQKWAMNNVSLALPTTPYLGAIKFGLNQSFDQSNPPETFPQDYDINRPPPNPATTI 454
            I G  KWA+NNVSLA+P TPYLG++K+ L   F++S+PP+ +P DYDI  PP N     
Sbjct: 381 LIEGFTKWAINNVSLAVPGTPYLGSVKYNLRTGFNRSSPPKDYPVDYDIMTPPRNRNAKQ 440

Query: 455 GNAIYRLDLGSTVDFILQNANTLTKNLSEIHPWHLHGHDFWVLGYGEGKFSAAADEKKLN 514
           GN         TVD ILQNAN L  N SEIHPWHLHGHDFWVLGYGEGKF    DEK  N
Sbjct: 441 GNVSCVFPFNVTVDVILQNANGLNANASEIHPWHLHGHDFWVLGYGEGKFKPGVDEKTYN 500

Query: 515 LKNPPLKNTVVIFPYGWTAVRFVADNPGVWIFHCHIEPHLHMGMGVIFAEGVDKVGEIPR 523
           LKNPPL+NTV ++PYGWTA+RFV DNPGVW FHCHIEPHLHMGMGV+FAEG++++G++P 
Sbjct: 501 LKNPPLRNTVALYPYGWTALRFVTDNPGVWFFHCHIEPHLHMGMGVVFAEGLNRIGKVPD 560

BLAST of Sgr011741 vs. ExPASy TrEMBL
Match: E7BBM8 (L-ascorbate oxidase OS=Cucumis sativus OX=3659 GN=aso PE=2 SV=1)

HSP 1 Score: 676.0 bits (1743), Expect = 1.9e-190
Identity = 343/580 (59.14%), Postives = 394/580 (67.93%), Query Frame = 0

Query: 3   IAAEP-FRRGFRVLLVIFFLLVSGFGLSEGS--KTRHFQWDVEYMFWSPDCVENVVMGIN 62
           +A +P F + F   LV+  +   G  LSE    K +H++WDVEYMFWSPDCVEN+VMGIN
Sbjct: 4   VADKPFFPKPFLSFLVLSIIFGFGITLSEAGFPKIKHYKWDVEYMFWSPDCVENIVMGIN 63

Query: 63  GQFPGPTIRANVGDTVVVDVTNKLFTEGVVIHWHGIRQIGTPWSDGTASISQCAINPGET 122
           G+FPGPTIRAN GD VVV++TNKL TEGVVIHWHGI Q GTPW+DGTASISQCAINPGET
Sbjct: 64  GEFPGPTIRANAGDIVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGET 123

Query: 123 FVYRFKVDKAGTYFYHGHYGMQRSAGLYGSLIVDVAEGEKEPFHYDQEINLLLSDWWHQS 182
           F YRF VDKAGTYFYHGH GMQRSAGLYGSLIVD  EG  EPFHYD+EINLLLSDWWHQS
Sbjct: 124 FTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPPEGRSEPFHYDEEINLLLSDWWHQS 183

Query: 183 VHEQEVGLSSSPTRWIGNPQSLLINGRGQFKCSIAAKYNISLNQCKLKGDDRHR----HL 242
           VH+QEVGLSS P RWIG PQS+LING+GQF CSIAAKYN  L QC+L G ++      H+
Sbjct: 184 VHKQEVGLSSKPMRWIGEPQSILINGKGQFDCSIAAKYNQGLKQCELSGKEKCAPFILHV 243

Query: 243 FRRELLRPH---------------NHQSRPIQQ-----------------------LLGF 302
             ++  R                 NH+   ++                        L+  
Sbjct: 244 QPKKTYRIRIASTTALASLNFAIGNHELLVVEADGNYVQPFVTSDIDIYSGESYSVLITT 303

Query: 303 RRRQRAKTGDPAGTHP--PQLPPQLRLQVACFSAAGHSPVGRLRPQQEL-------LQTN 362
            +          G     P+ PP L L     ++A   P+    P  E           N
Sbjct: 304 DQNPLENYWVSIGVRARLPKTPPGLTLLNYLPNSASKLPIS---PPPETPHWEDFDRSKN 363

Query: 363 LRRRRLCATAK-----NFSRRIFLLNTQNQINGVQKWAMNNVSLALPTTPYLGAIKFGLN 422
              R   A         ++RR+FLLNTQN+ING  KWA+NNVSLALP TPYL A+K  LN
Sbjct: 364 FTFRIFAAMGSPKPPVRYNRRLFLLNTQNRINGFMKWAINNVSLALPPTPYLAAMKMRLN 423

Query: 423 QSFDQSNPPETFPQDYDINRPPPNPATTIGNAIYRLDLGSTVDFILQNANTLTKNLSEIH 482
            +F+QS PPETFP +YDIN PPPNP TT GN +Y+ ++G TVD ILQNAN L  N+SEIH
Sbjct: 424 TAFNQSPPPETFPLNYDINNPPPNPETTTGNGVYKFNMGETVDVILQNANMLNPNMSEIH 483

Query: 483 PWHLHGHDFWVLGYGEGKFSAAADEKKLNLKNPPLKNTVVIFPYGWTAVRFVADNPGVWI 524
           PWHLHGHDFWVLGYGEGKF A  DEKKLNLKNPPL+NTVVIFPYGWTA+RFVADNPGVW 
Sbjct: 484 PWHLHGHDFWVLGYGEGKFYAPEDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWA 543

BLAST of Sgr011741 vs. ExPASy TrEMBL
Match: A0A0A0LXB9 (L-ascorbate oxidase OS=Cucumis sativus OX=3659 GN=Csa_1G604040 PE=3 SV=1)

HSP 1 Score: 674.9 bits (1740), Expect = 4.2e-190
Identity = 342/580 (58.97%), Postives = 394/580 (67.93%), Query Frame = 0

Query: 3   IAAEP-FRRGFRVLLVIFFLLVSGFGLSEGS--KTRHFQWDVEYMFWSPDCVENVVMGIN 62
           +A +P F + F   LV+  +   G  LSE    K +H++WDVEYMFWSPDCVEN+VMGIN
Sbjct: 4   VADKPFFPKPFLSFLVLSIIFGFGITLSEAGFPKIKHYKWDVEYMFWSPDCVENIVMGIN 63

Query: 63  GQFPGPTIRANVGDTVVVDVTNKLFTEGVVIHWHGIRQIGTPWSDGTASISQCAINPGET 122
           G+FPGPTIRAN GD VVV++TNKL TEGVVIHWHGI Q GTPW+DGTASISQCAINPGET
Sbjct: 64  GEFPGPTIRANAGDIVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGET 123

Query: 123 FVYRFKVDKAGTYFYHGHYGMQRSAGLYGSLIVDVAEGEKEPFHYDQEINLLLSDWWHQS 182
           F YRF VDKAGTYFYHGH GMQRSAGLYGSLIVD  EG  EPFHYD+EINLLLSDWWHQS
Sbjct: 124 FTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPPEGRSEPFHYDEEINLLLSDWWHQS 183

Query: 183 VHEQEVGLSSSPTRWIGNPQSLLINGRGQFKCSIAAKYNISLNQCKLKGDDRHR----HL 242
           VH+QEVGLSS P RWIG PQS+LING+GQF CSIAAKYN  L QC+L G ++      H+
Sbjct: 184 VHKQEVGLSSKPMRWIGEPQSILINGKGQFDCSIAAKYNQGLKQCELSGKEKCAPFILHV 243

Query: 243 FRRELLRPH---------------NHQSRPIQQ-----------------------LLGF 302
             ++  R                 NH+   ++                        L+  
Sbjct: 244 QPKKTYRIRIASTTALASLNFAIGNHELLVVEADGNYVQPFVTSDIDIYSGESYSVLITT 303

Query: 303 RRRQRAKTGDPAGTHP--PQLPPQLRLQVACFSAAGHSPVGRLRPQQEL-------LQTN 362
            +          G     P+ PP L L     ++A   P+    P  E           N
Sbjct: 304 DQNPLENYWVSIGVRARLPKTPPGLTLLNYLPNSASKLPIS---PPPETPHWEDFDRSKN 363

Query: 363 LRRRRLCATAK-----NFSRRIFLLNTQNQINGVQKWAMNNVSLALPTTPYLGAIKFGLN 422
              R   A         ++RR+FLLNTQN+ING  KWA+NNVSLALP TPYL A+K  LN
Sbjct: 364 FTFRIFAAMGSPKPPVRYNRRLFLLNTQNRINGFMKWAINNVSLALPPTPYLAAMKMRLN 423

Query: 423 QSFDQSNPPETFPQDYDINRPPPNPATTIGNAIYRLDLGSTVDFILQNANTLTKNLSEIH 482
            +F+Q+ PPETFP +YDIN PPPNP TT GN +Y+ ++G TVD ILQNAN L  N+SEIH
Sbjct: 424 TAFNQNPPPETFPLNYDINNPPPNPETTTGNGVYKFNMGETVDVILQNANMLNPNMSEIH 483

Query: 483 PWHLHGHDFWVLGYGEGKFSAAADEKKLNLKNPPLKNTVVIFPYGWTAVRFVADNPGVWI 524
           PWHLHGHDFWVLGYGEGKF A  DEKKLNLKNPPL+NTVVIFPYGWTA+RFVADNPGVW 
Sbjct: 484 PWHLHGHDFWVLGYGEGKFYAPEDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWA 543

BLAST of Sgr011741 vs. ExPASy TrEMBL
Match: Q9M5B4 (L-ascorbate oxidase OS=Cucumis melo OX=3656 GN=LOC103492479 PE=2 SV=1)

HSP 1 Score: 674.1 bits (1738), Expect = 7.2e-190
Identity = 344/580 (59.31%), Postives = 397/580 (68.45%), Query Frame = 0

Query: 3   IAAEP-FRRGFRVLLVIFFLLVSGFGLSEGS--KTRHFQWDVEYMFWSPDCVENVVMGIN 62
           +A +P F + F V  V+  +   G  LSE    K RH++W+VEYMFWSPDCVEN+VMGIN
Sbjct: 4   VADKPFFPKPFLVFFVLSIIFGFGITLSEAGFPKIRHYKWEVEYMFWSPDCVENIVMGIN 63

Query: 63  GQFPGPTIRANVGDTVVVDVTNKLFTEGVVIHWHGIRQIGTPWSDGTASISQCAINPGET 122
           GQFPGPTIRAN GD VVV++TNKL TEGVVIHWHGI Q GTPW+DGTASISQCAINPGET
Sbjct: 64  GQFPGPTIRANAGDMVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGET 123

Query: 123 FVYRFKVDKAGTYFYHGHYGMQRSAGLYGSLIVDVAEGEKEPFHYDQEINLLLSDWWHQS 182
           F YRF VDKAGTYFYHGH GMQRSAGLYGSLIVD  EG  EPFHYD+EINLLLSDWWHQS
Sbjct: 124 FTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPQEGTSEPFHYDEEINLLLSDWWHQS 183

Query: 183 VHEQEVGLSSSPTRWIGNPQSLLINGRGQFKCSIAAKYNISLNQCKLKGDDRHR----HL 242
           VH+QEVGLSS P RWIG PQS+LINGRGQF CSIAAKY   L QC+L G ++      H+
Sbjct: 184 VHKQEVGLSSKPMRWIGEPQSILINGRGQFDCSIAAKYKNGLKQCELSGKEQCAPFILHV 243

Query: 243 FRRELLRPHNHQSRPIQQL---LGFRRR----------QRAKTGD--------------- 302
             +++ R     +  +  L   +G  +           Q   T D               
Sbjct: 244 HPKKIYRIRIASTTALASLNFAIGNHKLLVVEADGNYVQPFSTSDIDIYSGESYSVLITT 303

Query: 303 ---PAGTH---------PPQLPPQLRLQVACFSAAGHSPVGRLRPQQEL-------LQTN 362
              P+  +          P+ PP L +     ++    P+    P  E           N
Sbjct: 304 DQNPSENYWVSIGVRARLPKTPPGLTVLNYLPNSVSKLPIS---PPPETPDWEDFDRSKN 363

Query: 363 LRRRRLCATAK-----NFSRRIFLLNTQNQINGVQKWAMNNVSLALPTTPYLGAIKFGLN 422
              R   A         ++RR+FLLNTQN+ING  KWA+NNVSLALP TPYL A+KF L+
Sbjct: 364 FTFRIFAAMGSPKPPVRYNRRLFLLNTQNRINGFMKWAINNVSLALPPTPYLAAMKFRLS 423

Query: 423 QSFDQSNPPETFPQDYDINRPPPNPATTIGNAIYRLDLGSTVDFILQNANTLTKNLSEIH 482
            +F+Q+ PPETFP DYDIN PP NP T  GN +Y+ ++G TVD ILQNAN L  N SEIH
Sbjct: 424 TAFNQNPPPETFPIDYDINNPPRNPETATGNGVYKFNMGETVDVILQNANMLNANTSEIH 483

Query: 483 PWHLHGHDFWVLGYGEGKFSAAADEKKLNLKNPPLKNTVVIFPYGWTAVRFVADNPGVWI 524
           PWHLHGHDFWVLGYGEGKFSAA D +KLNLKNPPL+NTVVIFPYGWTA+RFVADNPGVW 
Sbjct: 484 PWHLHGHDFWVLGYGEGKFSAAEDGRKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWA 543

BLAST of Sgr011741 vs. ExPASy TrEMBL
Match: A0A5A7UMY3 (L-ascorbate oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold181G00010 PE=3 SV=1)

HSP 1 Score: 672.9 bits (1735), Expect = 1.6e-189
Identity = 344/580 (59.31%), Postives = 396/580 (68.28%), Query Frame = 0

Query: 3   IAAEP-FRRGFRVLLVIFFLLVSGFGLSEGS--KTRHFQWDVEYMFWSPDCVENVVMGIN 62
           +A +P F + F V  V+  +   G  LSE    K RH++W+VEYMFWSPDCVEN+VMGIN
Sbjct: 4   VADKPFFPKPFLVFFVLSIIFGFGITLSEAGFPKIRHYKWEVEYMFWSPDCVENIVMGIN 63

Query: 63  GQFPGPTIRANVGDTVVVDVTNKLFTEGVVIHWHGIRQIGTPWSDGTASISQCAINPGET 122
           GQFPGPTIRAN GD VVV++TNKL TEGVVIHWHGI Q GTPW+DGTASISQCAINPGET
Sbjct: 64  GQFPGPTIRANAGDMVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGET 123

Query: 123 FVYRFKVDKAGTYFYHGHYGMQRSAGLYGSLIVDVAEGEKEPFHYDQEINLLLSDWWHQS 182
           F YRF VDKAGTYFYHGH GMQRSAGLYGSLIVD  EG  EPFHYD+EINLLLSDWWHQS
Sbjct: 124 FTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPQEGTSEPFHYDEEINLLLSDWWHQS 183

Query: 183 VHEQEVGLSSSPTRWIGNPQSLLINGRGQFKCSIAAKYNISLNQCKLKGDDRHR----HL 242
           VH+QEVGLSS P RWIG PQS+LINGRGQF CSIAAKY   L QC+L G ++      H+
Sbjct: 184 VHKQEVGLSSKPMRWIGEPQSILINGRGQFDCSIAAKYKNGLKQCELSGKEQCAPFILHV 243

Query: 243 FRRELLRPHNHQSRPIQQL---LGFRRR----------QRAKTGD--------------- 302
             ++  R     +  +  L   +G  +           Q   T D               
Sbjct: 244 HPKKTYRIRIASTTALASLNFAIGNHKLLVVEADGNYVQPFSTSDIDIYSGESYSVLITT 303

Query: 303 ---PAGTH---------PPQLPPQLRLQVACFSAAGHSPVGRLRPQQEL-------LQTN 362
              P+  +          P+ PP L +     ++    P+    P  E           N
Sbjct: 304 DQNPSENYWVSIGVRARLPKTPPGLTVLNYLPNSVSKLPIS---PPPETPDWEDFDRSKN 363

Query: 363 LRRRRLCATAK-----NFSRRIFLLNTQNQINGVQKWAMNNVSLALPTTPYLGAIKFGLN 422
              R   A         ++RR+FLLNTQN+ING  KWA+NNVSLALP TPYL A+KF L+
Sbjct: 364 FTFRIFAAMGSPKPPVRYNRRLFLLNTQNRINGFMKWAINNVSLALPPTPYLAAMKFRLS 423

Query: 423 QSFDQSNPPETFPQDYDINRPPPNPATTIGNAIYRLDLGSTVDFILQNANTLTKNLSEIH 482
            +F+Q+ PPETFP DYDIN PP NP T  GN +Y+ ++G TVD ILQNAN L  N SEIH
Sbjct: 424 TAFNQNPPPETFPIDYDINNPPRNPETATGNGVYKFNMGETVDVILQNANMLNANTSEIH 483

Query: 483 PWHLHGHDFWVLGYGEGKFSAAADEKKLNLKNPPLKNTVVIFPYGWTAVRFVADNPGVWI 524
           PWHLHGHDFWVLGYGEGKFSAA D +KLNLKNPPL+NTVVIFPYGWTA+RFVADNPGVW 
Sbjct: 484 PWHLHGHDFWVLGYGEGKFSAAEDGRKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWA 543

BLAST of Sgr011741 vs. ExPASy TrEMBL
Match: A0A6J1H925 (L-ascorbate oxidase OS=Cucurbita moschata OX=3662 GN=LOC111461608 PE=3 SV=1)

HSP 1 Score: 667.5 bits (1721), Expect = 6.7e-188
Identity = 340/585 (58.12%), Postives = 401/585 (68.55%), Query Frame = 0

Query: 3   IAAEPFRRGFRVLLVIFFLLVSGFGLSEGSKTRHFQWDVEYMFWSPDCVENVVMGINGQF 62
           +A  PF      LL++F   ++        K +H++W+VEYMFWSPDCVEN+VMGINGQF
Sbjct: 4   VAGHPF------LLILFLFSIAITPSIALPKIKHYKWEVEYMFWSPDCVENIVMGINGQF 63

Query: 63  PGPTIRANVGDTVVVDVTNKLFTEGVVIHWHGIRQIGTPWSDGTASISQCAINPGETFVY 122
           PGPTIRANVGDTVVV++TNKL TEGVVIHWHGI Q G+PW+DGTASISQCAINPGETF Y
Sbjct: 64  PGPTIRANVGDTVVVELTNKLHTEGVVIHWHGILQRGSPWADGTASISQCAINPGETFTY 123

Query: 123 RFKVDKAGTYFYHGHYGMQRSAGLYGSLIVDVAEGEKEPFHYDQEINLLLSDWWHQSVHE 182
           RF VDKAGT+FYHGH GMQRSAGLYGSLIVD  EG+ EPFHYD+EINLLLSDWWHQSVH+
Sbjct: 124 RFVVDKAGTFFYHGHLGMQRSAGLYGSLIVDPEEGKNEPFHYDEEINLLLSDWWHQSVHK 183

Query: 183 QEVGLSSSPTRWIGNPQSLLINGRGQFKCSIAAKYNISLNQCKLKGDDR------HRHLF 242
           QEVGLSS+P RWIG PQ++LINGRGQF CSIAAKY+     C LKG ++      H H  
Sbjct: 184 QEVGLSSNPMRWIGEPQTILINGRGQFNCSIAAKYHSGSKPCALKGGEQCAPFIMHVHPN 243

Query: 243 RRELLRPH-------------NHQSRPIQQLLGFRRRQRAKTGD---------------- 302
           +   +R               NHQ   ++    + +  R    D                
Sbjct: 244 KTVRIRISSTTALASLNFAIGNHQLLVVEADGNYVQPFRTSDIDIYSGESYSVLITTDQN 303

Query: 303 PAGTH---------PPQLPPQLRLQVACFSAAGHSPVGRLRPQQELLQTNLRRR-----R 362
           P+  +          P  PP L L     ++    P+  L P +     +  R      R
Sbjct: 304 PSENYWVSIGVRARRPNTPPGLTLLNYLPNSPAKLPI--LPPPETPDWEDFDRSKNFTFR 363

Query: 363 LCATAKN------FSRRIFLLNTQNQINGVQKWAMNNVSLALPTTPYLGAIKFGLNQSFD 422
           + +T  +      F RRIFLLNTQN++NG  KWA+NNVSLALP TPYLGA+K+ L  +F+
Sbjct: 364 IFSTMSSPKPPTRFDRRIFLLNTQNRLNGFTKWAINNVSLALPPTPYLGAMKYRLKTAFN 423

Query: 423 QSNPPETFPQDYDINRPPPNPATTIGNAIYRLDLGSTVDFILQNANTLTKNLSEIHPWHL 482
           Q+ PPE FP +Y I+ PPPNP TTIGN +YR  LG TVD ILQNAN L  N SEIHPWHL
Sbjct: 424 QNPPPENFPSNYSIDIPPPNPNTTIGNGVYRFKLGETVDVILQNANMLNPNTSEIHPWHL 483

Query: 483 HGHDFWVLGYGEGKFSAAADEKKLNLKNPPLKNTVVIFPYGWTAVRFVADNPGVWIFHCH 527
           HGHDFWVLGYG+GKFS A   +KLNLKNPPL+NTVVIFP GWTA+RFVADNPGVW FHCH
Sbjct: 484 HGHDFWVLGYGDGKFSTAVHGRKLNLKNPPLRNTVVIFPNGWTAIRFVADNPGVWAFHCH 543

BLAST of Sgr011741 vs. TAIR 10
Match: AT5G21105.1 (Plant L-ascorbate oxidase )

HSP 1 Score: 570.9 bits (1470), Expect = 1.7e-162
Identity = 279/560 (49.82%), Postives = 354/560 (63.21%), Query Frame = 0

Query: 21  LLVSGFGLSEGSKTRHFQWDVEYMFWSPDCVENVVMGINGQFPGPTIRANVGDTVVVDVT 80
           L+V+    +  +  R + W+VEY +WSPDC E  VM +NG+FPGPTI+A  GDT+VV++T
Sbjct: 24  LVVAVLTHTASAAVREYHWEVEYKYWSPDCKEGAVMTVNGEFPGPTIKAFAGDTIVVNLT 83

Query: 81  NKLFTEGVVIHWHGIRQIGTPWSDGTASISQCAINPGETFVYRFKVDKAGTYFYHGHYGM 140
           NKL TEG+VIHWHGIRQ G+PW+DG A ++QCAINPGETF Y F V+K GT+FYHGHYGM
Sbjct: 84  NKLTTEGLVIHWHGIRQFGSPWADGAAGVTQCAINPGETFTYNFTVEKPGTHFYHGHYGM 143

Query: 141 QRSAGLYGSLIVDVAEGEKEPFHYDQEINLLLSDWWHQSVHEQEVGLSSSPTRWIGNPQS 200
           QRSAGLYGSLIVDVA+G+ E   YD E NLLLSDWWH+++  QE+GLSS P RWIG  QS
Sbjct: 144 QRSAGLYGSLIVDVAKGKSERLRYDGEFNLLLSDWWHEAIPSQELGLSSKPMRWIGEAQS 203

Query: 201 LLINGRGQFKCSIAAKY--NISLNQCKLKGDDRHR----HLFRRELLRPHNHQSRPIQQL 260
           +LINGRGQF CS+AA++  N SL  C  K  D+      H+   +  R     +  +  L
Sbjct: 204 ILINGRGQFNCSLAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTYRIRLSSTTALASL 263

Query: 261 LGFRRRQRAKTGDPAGTHPPQLPPQLRLQVACFSAAGHS---PVGRLRPQQELLQTNLRR 320
               +  +    +  G +   + P     +  +S   +S      +   Q   +   +R 
Sbjct: 264 NLAVQGHKLVVVEADGNY---ITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYISVGVRG 323

Query: 321 RRLCAT--------------------------------AKNFSRRIF------------- 380
           R+   T                                +KNFS++IF             
Sbjct: 324 RKPNTTQALTILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIFSAMGSPSPPKKYR 383

Query: 381 ----LLNTQNQINGVQKWAMNNVSLALPTTPYLGAIKFGLNQSFDQSNPPETFPQDYDIN 440
               LLNTQN I+G  KWA+NNVSL  P TPYLG++K+ L   F++ +PP ++  DYDI 
Sbjct: 384 KRLILLNTQNLIDGYTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRMDYDIM 443

Query: 441 RPPPNPATTIGNAIYRLDLGSTVDFILQNANTLTKNLSEIHPWHLHGHDFWVLGYGEGKF 500
            PPP P TT GN IY      TVD I+QNAN L   +SEIHPWHLHGHDFWVLGYG+GKF
Sbjct: 444 NPPPFPNTTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLGYGDGKF 503

Query: 501 SAAADEKKLNLKNPPLKNTVVIFPYGWTAVRFVADNPGVWIFHCHIEPHLHMGMGVIFAE 523
               DEK  NLKNPPL+NT +++PYGWTA+RFV DNPGVW FHCHIEPHLHMGMGV+FAE
Sbjct: 504 KPGIDEKTYNLKNPPLRNTAILYPYGWTAIRFVTDNPGVWFFHCHIEPHLHMGMGVVFAE 563

BLAST of Sgr011741 vs. TAIR 10
Match: AT5G21100.1 (Plant L-ascorbate oxidase )

HSP 1 Score: 535.8 bits (1379), Expect = 5.9e-152
Identity = 279/546 (51.10%), Postives = 331/546 (60.62%), Query Frame = 0

Query: 39  WDVEYMFWSPDCVENVVMGINGQFPGPTIRANVGDTVVVDVTNKLFTEGVVIHWHGIRQI 98
           W+VEY +W PDC E +VM INGQFPGPTI A  GDTV++ V NKL TEGVVIHWHGIRQ 
Sbjct: 28  WEVEYKYWWPDCKEGIVMAINGQFPGPTIDAVAGDTVIIHVVNKLSTEGVVIHWHGIRQK 87

Query: 99  GTPWSDGTASISQCAINPGETFVYRFKVDKAGTYFYHGHYGMQRSAGLYGSLIVDVAEGE 158
           GTPW+DG A ++QC INPGETF Y+F VDKAGT+FYHGHYGMQRS+GLYG LIV      
Sbjct: 88  GTPWADGAAGVTQCPINPGETFTYKFIVDKAGTHFYHGHYGMQRSSGLYGMLIV---RSP 147

Query: 159 KEPFHYDQEINLLLSDWWHQSVHEQEVGLSSSPTRWIGNPQSLLINGRGQFKCSIAAKYN 218
           KE   YD E NLLLSDWWHQS+H QE+ LSS P RWIG PQSLLINGRGQF CS AA +N
Sbjct: 148 KERLIYDGEFNLLLSDWWHQSIHAQELALSSRPMRWIGEPQSLLINGRGQFNCSQAAYFN 207

Query: 219 I--SLNQCKLKGDDR---------HRHLFRREL----------LRPHNHQSRPIQQ---- 278
                + C  K +D+            ++R  +          L    HQ   ++     
Sbjct: 208 KGGEKDVCTFKENDQCAPQTLRVEPNRVYRLRIASTTALASLNLAVQGHQLVVVEADGNY 267

Query: 279 -------------------LLGFRRRQRAKTGDPAGTHPPQLPPQLRLQVACFSAAGHSP 338
                              LL        K     G    +      L V  +  A  S 
Sbjct: 268 VAPFTVNDIDVYSGETYSVLLKTNALPSKKYWISVGVRGREPKTPQALTVINYVDATESR 327

Query: 339 VGRLRPQQELLQTNLRRRRLCATAKNFSRRIF-----------------LLNTQNQINGV 398
                P   +     R       +K+FS++IF                 LLNTQN     
Sbjct: 328 PSHPPPVTPIWNDTDR-------SKSFSKKIFAAKGYPKPPEKSHDQLILLNTQNLYEDY 387

Query: 399 QKWAMNNVSLALPTTPYLGAIKFGLNQSFDQSNPPETFPQD-YDINRPPPNPATTIGNAI 458
            KW++NNVSL++P TPYLG+I++GL  ++D  +P +    D YDI +PPPNP TT G+ I
Sbjct: 388 TKWSINNVSLSVPVTPYLGSIRYGLKSAYDLKSPAKKLIMDNYDIMKPPPNPNTTKGSGI 447

Query: 459 YRLDLGSTVDFILQNANTLTKNLSEIHPWHLHGHDFWVLGYGEGKFSAAADEKKLNLKNP 518
           Y    G  VD ILQNAN L   +SEIHPWH+HGHDFWVLGYGEGKF    DEK  NLKNP
Sbjct: 448 YNFAFGIVVDVILQNANVLKGVISEIHPWHIHGHDFWVLGYGEGKFKPGIDEKTFNLKNP 507

Query: 519 PLKNTVVIFPYGWTAVRFVADNPGVWIFHCHIEPHLHMGMGVIFAEGVDKVG--EIPRSA 521
           PL+NTVV++P+GWTA+RFV DNPGVW FHCHIEPHLHMGMGV+F EGVD++G  EIP  A
Sbjct: 508 PLRNTVVLYPFGWTAIRFVTDNPGVWFFHCHIEPHLHMGMGVVFVEGVDRIGKMEIPDEA 563

BLAST of Sgr011741 vs. TAIR 10
Match: AT5G21105.3 (Plant L-ascorbate oxidase )

HSP 1 Score: 496.9 bits (1278), Expect = 3.0e-140
Identity = 249/518 (48.07%), Postives = 317/518 (61.20%), Query Frame = 0

Query: 21  LLVSGFGLSEGSKTRHFQWDVEYMFWSPDCVENVVMGINGQFPGPTIRANVGDTVVVDVT 80
           L+V+    +  +  R + W+VEY +WSPDC E  VM +NG+FPGPTI+A  GDT+VV++T
Sbjct: 24  LVVAVLTHTASAAVREYHWEVEYKYWSPDCKEGAVMTVNGEFPGPTIKAFAGDTIVVNLT 83

Query: 81  NKLFTEGVVIHWHGIRQIGTPWSDGTASISQCAINPGETFVYRFKVDKAGTYFYHGHYGM 140
           NKL TEG+VIHWHGIRQ G+PW+DG A ++QCAINPGETF Y F V+K GT+FYHGHYGM
Sbjct: 84  NKLTTEGLVIHWHGIRQFGSPWADGAAGVTQCAINPGETFTYNFTVEKPGTHFYHGHYGM 143

Query: 141 QRSAGLYGSLIVDVAEGEKEPFHYDQEINLLLSDWWHQSVHEQEVGLSSSPTRWIGNPQS 200
           QRSAGLYGSLIVDVA+G+ E   YD E NLLLSDWWH+++  QE+GLSS P RWIG  QS
Sbjct: 144 QRSAGLYGSLIVDVAKGKSERLRYDGEFNLLLSDWWHEAIPSQELGLSSKPMRWIGEAQS 203

Query: 201 LLINGRGQFKCSIAAKY--NISLNQCKLKGDDRHR----HLFRRELLRPHNHQSRPIQQL 260
           +LINGRGQF CS+AA++  N SL  C  K  D+      H+   +  R     +  +  L
Sbjct: 204 ILINGRGQFNCSLAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTYRIRLSSTTALASL 263

Query: 261 LGFRRRQRAKTGDPAGTHPPQLPPQLRLQVACFSAAGHS---PVGRLRPQQELLQTNLRR 320
               +  +    +  G +   + P     +  +S   +S      +   Q   +   +R 
Sbjct: 264 NLAVQGHKLVVVEADGNY---ITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYISVGVRG 323

Query: 321 RRLCAT--------------------------------AKNFSRRIF------------- 380
           R+   T                                +KNFS++IF             
Sbjct: 324 RKPNTTQALTILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIFSAMGSPSPPKKYR 383

Query: 381 ----LLNTQNQINGVQKWAMNNVSLALPTTPYLGAIKFGLNQSFDQSNPPETFPQDYDIN 440
               LLNTQN I+G  KWA+NNVSL  P TPYLG++K+ L   F++ +PP ++  DYDI 
Sbjct: 384 KRLILLNTQNLIDGYTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRMDYDIM 443

Query: 441 RPPPNPATTIGNAIYRLDLGSTVDFILQNANTLTKNLSEIHPWHLHGHDFWVLGYGEGKF 481
            PPP P TT GN IY      TVD I+QNAN L   +SEIHPWHLHGHDFWVLGYG+GKF
Sbjct: 444 NPPPFPNTTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLGYGDGKF 503

BLAST of Sgr011741 vs. TAIR 10
Match: AT4G39830.1 (Cupredoxin superfamily protein )

HSP 1 Score: 458.4 bits (1178), Expect = 1.2e-128
Identity = 247/573 (43.11%), Postives = 326/573 (56.89%), Query Frame = 0

Query: 12  FRVLLVIFFLLVSGFGLSEGSKTRHFQWDVEYMFWSPDCVENVVMGINGQFPGPTIRANV 71
           F ++++ F  L     L +G K R F+W+V+Y F SPDC E +V+ ING+FPGPTI+A  
Sbjct: 14  FNLMVLCFIALFFSSVLCQG-KIRRFKWEVKYEFKSPDCFEKLVITINGKFPGPTIKAQQ 73

Query: 72  GDTVVVDVTNKLFTEGVVIHWHGIRQIGTPWSDGTASISQCAINPGETFVYRFKVDKAGT 131
           GDT+VV++ N   TE V +HWHGIRQIGTPW DG   ++QC I PGE F+Y+F VD+ GT
Sbjct: 74  GDTIVVELKNSFMTENVAVHWHGIRQIGTPWFDGVEGVTQCPILPGEVFIYQFVVDRPGT 133

Query: 132 YFYHGHYGMQRSAGLYGSLIVDVAEGEKEPFHYDQEINLLLSDWWHQSVHEQEVGLSSSP 191
           Y YH HYGMQR +GL G + V     E EPF YD + N LL+DW+H+S+ E+  GL+S P
Sbjct: 134 YMYHSHYGMQRESGLIGMIQVSPPATEPEPFTYDYDRNFLLTDWYHKSMSEKATGLASIP 193

Query: 192 TRWIGNPQSLLINGRGQFKCS--IAAKYNISLNQCKLKGDDRHRHL--------FRREL- 251
            +W+G PQSL+I GRG+F CS  +    ++    C +   D  R +        +R  + 
Sbjct: 194 FKWVGEPQSLMIQGRGRFNCSNNLTTPPSLVSGVCNVSNADCSRFILTVIPGKTYRLRIG 253

Query: 252 -----------LRPHN--------HQSRP--IQQLLGF---------------RRR---- 311
                      +  HN        H   P  ++ L  +               RR     
Sbjct: 254 SLTALSALSFQIEGHNLTVVEADGHYVEPFTVKNLFVYSGETYSVLLKADQNPRRNYWIT 313

Query: 312 ----QRAKTGDPA-------GTHPPQLPPQLRLQVACFSAAGHSPVGRLRPQQELLQTNL 371
                R  T  PA         HP + PP    + +      +    RL    + L    
Sbjct: 314 SSIVSRPATTPPATAVLNYYPNHPRRRPP--TSESSNIVPEWNDTRSRL---AQSLAIKA 373

Query: 372 RRRRLCATAKNFSRRIFLLNTQNQINGVQKWAMNNVSLALPTTPYLGAIKFGLNQSFD-Q 431
           RR  + A  +N  + I LLNTQN++NG ++W++NNVS   P TPYL A+K  L  +FD +
Sbjct: 374 RRGFIHALPENSDKVIVLLNTQNEVNGYRRWSVNNVSYHHPKTPYLIALKQNLTNAFDWR 433

Query: 432 SNPPETF-PQDYDINRPPPNPATTIGNAIYRLDLGSTVDFILQNANTLTKNLSEIHPWHL 491
              PE +  ++YDI   P N   T  + IYRL   STVD ILQNANT+  N SE HPWHL
Sbjct: 434 FTAPENYDSRNYDIFAKPLNANATTSDGIYRLRFNSTVDVILQNANTMNANNSETHPWHL 493

Query: 492 HGHDFWVLGYGEGKFSAAADEKKLNLKNPPLKNTVVIFPYGWTAVRFVADNPGVWIFHCH 521
           HGHDFWVLGYGEGKF+ + D K+ N  +P  KNTV + P+GWTA+RF ADNPGVW FHCH
Sbjct: 494 HGHDFWVLGYGEGKFNESEDPKRYNRVDPIKKNTVAVQPFGWTALRFRADNPGVWSFHCH 553

BLAST of Sgr011741 vs. TAIR 10
Match: AT5G21105.2 (Plant L-ascorbate oxidase )

HSP 1 Score: 303.9 bits (777), Expect = 3.8e-82
Identity = 129/204 (63.24%), Postives = 157/204 (76.96%), Query Frame = 0

Query: 319 KNFSRRIFLLNTQNQINGVQKWAMNNVSLALPTTPYLGAIKFGLNQSFDQSNPPETFPQD 378
           K + +R+ LLNTQN I+G  KWA+NNVSL  P TPYLG++K+ L   F++ +PP ++  D
Sbjct: 186 KKYRKRLILLNTQNLIDGYTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRMD 245

Query: 379 YDINRPPPNPATTIGNAIYRLDLGSTVDFILQNANTLTKNLSEIHPWHLHGHDFWVLGYG 438
           YDI  PPP P TT GN IY      TVD I+QNAN L   +SEIHPWHLHGHDFWVLGYG
Sbjct: 246 YDIMNPPPFPNTTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLGYG 305

Query: 439 EGKFSAAADEKKLNLKNPPLKNTVVIFPYGWTAVRFVADNPGVWIFHCHIEPHLHMGMGV 498
           +GKF    DEK  NLKNPPL+NT +++PYGWTA+RFV DNPGVW FHCHIEPHLHMGMGV
Sbjct: 306 DGKFKPGIDEKTYNLKNPPLRNTAILYPYGWTAIRFVTDNPGVWFFHCHIEPHLHMGMGV 365

Query: 499 IFAEGVDKVGEIPRSALSCGLTGQ 523
           +FAEG++++G++P  AL CGLT Q
Sbjct: 366 VFAEGLNRIGKVPDEALGCGLTKQ 389

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038878978.15.5e-19261.52L-ascorbate oxidase [Benincasa hispida][more]
XP_038880052.15.5e-19261.38L-ascorbate oxidase-like [Benincasa hispida][more]
CBY84386.13.9e-19059.14ascorbate oxidase [Cucumis sativus][more]
XP_004149015.18.7e-19058.97L-ascorbate oxidase [Cucumis sativus] >P14133.1 RecName: Full=L-ascorbate oxidas... [more]
NP_001284441.11.5e-18959.31L-ascorbate oxidase [Cucumis melo] >AAF35911.2 ascorbate oxidase AO4 [Cucumis me... [more]
Match NameE-valueIdentityDescription
P141331.1e-19258.97L-ascorbate oxidase OS=Cucumis sativus OX=3659 PE=1 SV=1[more]
P247921.4e-18558.23L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=AAO PE=1 SV=2[more]
P370641.8e-18258.44L-ascorbate oxidase OS=Cucurbita pepo var. melopepo OX=3665 PE=1 SV=1[more]
Q405889.7e-17655.96L-ascorbate oxidase OS=Nicotiana tabacum OX=4097 GN=AAO PE=2 SV=1[more]
M4DUF24.8e-15951.45L-ascorbate oxidase OS=Brassica rapa subsp. pekinensis OX=51351 GN=AO PE=2 SV=1[more]
Match NameE-valueIdentityDescription
E7BBM81.9e-19059.14L-ascorbate oxidase OS=Cucumis sativus OX=3659 GN=aso PE=2 SV=1[more]
A0A0A0LXB94.2e-19058.97L-ascorbate oxidase OS=Cucumis sativus OX=3659 GN=Csa_1G604040 PE=3 SV=1[more]
Q9M5B47.2e-19059.31L-ascorbate oxidase OS=Cucumis melo OX=3656 GN=LOC103492479 PE=2 SV=1[more]
A0A5A7UMY31.6e-18959.31L-ascorbate oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold181G... [more]
A0A6J1H9256.7e-18858.12L-ascorbate oxidase OS=Cucurbita moschata OX=3662 GN=LOC111461608 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G21105.11.7e-16249.82Plant L-ascorbate oxidase [more]
AT5G21100.15.9e-15251.10Plant L-ascorbate oxidase [more]
AT5G21105.33.0e-14048.07Plant L-ascorbate oxidase [more]
AT4G39830.11.2e-12843.11Cupredoxin superfamily protein [more]
AT5G21105.23.8e-8263.24Plant L-ascorbate oxidase [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003441NAC domainPFAMPF02365NAMcoord: 554..684
e-value: 9.7E-22
score: 78.2
IPR003441NAC domainPROSITEPS51005NACcoord: 552..711
score: 30.327198
IPR011706Multicopper oxidase, C-terminalPFAMPF07731Cu-oxidase_2coord: 390..504
e-value: 6.5E-36
score: 123.1
IPR008972CupredoxinGENE3D2.60.40.420coord: 27..153
e-value: 2.3E-48
score: 165.9
IPR008972CupredoxinGENE3D2.60.40.420coord: 162..271
e-value: 6.1E-18
score: 67.6
IPR008972CupredoxinGENE3D2.60.40.420coord: 316..524
e-value: 6.6E-75
score: 253.4
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 319..517
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 31..178
IPR001117Multicopper oxidase, type 1PFAMPF00394Cu-oxidasecoord: 169..233
e-value: 2.5E-9
score: 37.4
IPR036093NAC domain superfamilyGENE3D2.170.150.80NAC domaincoord: 561..715
e-value: 2.6E-27
score: 97.5
IPR036093NAC domain superfamilySUPERFAMILY101941NAC domaincoord: 551..710
IPR011707Multicopper oxidase, N-termianlPFAMPF07732Cu-oxidase_3coord: 41..154
e-value: 8.1E-42
score: 142.0
NoneNo IPR availablePANTHERPTHR11709:SF310L-ASCORBATE OXIDASEcoord: 320..522
NoneNo IPR availablePANTHERPTHR11709:SF310L-ASCORBATE OXIDASEcoord: 17..223
IPR045087Multicopper oxidasePANTHERPTHR11709MULTI-COPPER OXIDASEcoord: 320..522
IPR045087Multicopper oxidasePANTHERPTHR11709MULTI-COPPER OXIDASEcoord: 17..223
IPR002355Multicopper oxidase, copper-binding sitePROSITEPS00080MULTICOPPER_OXIDASE2coord: 485..496
IPR033138Multicopper oxidases, conserved sitePROSITEPS00079MULTICOPPER_OXIDASE1coord: 480..500
IPR034267Ascorbate oxidase, third cupredoxin domainCDDcd13893CuRO_3_AAOcoord: 321..511
e-value: 2.37615E-84
score: 263.126
IPR034259Ascorbate oxidase, first cupredoxin domainCDDcd13845CuRO_1_AAOcoord: 35..154
e-value: 1.27846E-74
score: 236.188

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr011741.1Sgr011741.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
molecular_function GO:0005507 copper ion binding
molecular_function GO:0003677 DNA binding
molecular_function GO:0016491 oxidoreductase activity