Sgr011532 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr011532 vs. NCBI nr
Match: XP_022148217.1 (acetyl-coenzyme A synthetase, chloroplastic/glyoxysomal isoform X1 [Momordica charantia])

HSP 1 Score: 1492.6 bits (3863), Expect = 0.0e+00
Identity = 733/797 (91.97%), Postives = 754/797 (94.60%), Query Frame = 0

Query: 3   NTVATSS-YGRPFPASFNPTLIIFPSAAIPLPNNSNPKIHNRKQLNYNQRHGHVNTFHNY 62
           +TVATSS + RPFPA  NPT  +FPSA+    NNS  KIHN KQ NY +R   VNTFH+Y
Sbjct: 4   HTVATSSCFIRPFPA--NPT-FLFPSAS--SANNSVHKIHNWKQPNYKKR--QVNTFHSY 63

Query: 63  YYLNSCLPPSCCARSFICSSWTSASGFCCSLSRTSGEQDKVAMGFTDVTASDRLTHLDSS 122
           YY NSCL PS CARSF      S+SGFCCS SRTSGEQ +V MGFTD+TA DRLT +DSS
Sbjct: 64  YYFNSCLRPS-CARSF--GFTRSSSGFCCSFSRTSGEQAQVVMGFTDLTAPDRLTQVDSS 123

Query: 123 AARKIPRLNATVLGESLASEDDQLILPSDHFSSRAHVSSHHKYLEMYKRSIEDPAGFWSD 182
           AARKIPRLNATVLGESLASEDDQLILPSD FSSRAHVSSH KYLEMYKRSIEDPAGFWSD
Sbjct: 124 AARKIPRLNATVLGESLASEDDQLILPSDQFSSRAHVSSHQKYLEMYKRSIEDPAGFWSD 183

Query: 183 IASSEFFWKHTWDQQVYSENLDVRKGQIKIEWFKGGITNICYNCLDRNIEAGLGEKVALY 242
           IASSEFFWKH WDQ VYSENLDVRKG+IKIEWFKGGITNICYNCLDRNIEAGLG+KVALY
Sbjct: 184 IASSEFFWKHRWDQLVYSENLDVRKGEIKIEWFKGGITNICYNCLDRNIEAGLGDKVALY 243

Query: 243 WEGNEPCFDGTLTYTQLLHKVCQLANFLKDNGVRKGDAVVIYMPMLMELPIAMLACARIG 302
           WEGNEP FDGTLTYT+LLHKVCQLAN+LKDNGV+KGDAVVIYMPMLMELPIAMLACARIG
Sbjct: 244 WEGNEPGFDGTLTYTELLHKVCQLANYLKDNGVKKGDAVVIYMPMLMELPIAMLACARIG 303

Query: 303 AIHSVVFAGFSSESLAQRIIDCKPKIVITCNAVKRGSKVIHLKDIVDAALTESAQNEVSV 362
           AIHSVVFAGFSSESLAQRIIDCKPKIVITCNAVKRGSKVIHLKDIVDAALTESAQNEVSV
Sbjct: 304 AIHSVVFAGFSSESLAQRIIDCKPKIVITCNAVKRGSKVIHLKDIVDAALTESAQNEVSV 363

Query: 363 ATCLTYENESALKSESTKWKEGRDIWWQDVVPTYPTTCTVEWVDAEHPLFLLYTSGSTGK 422
           ATCLTYENESALK EST WKEGRDIWWQDVVPTYPTTCTVEWVDAE PLFLLYTSGSTGK
Sbjct: 364 ATCLTYENESALKRESTTWKEGRDIWWQDVVPTYPTTCTVEWVDAEFPLFLLYTSGSTGK 423

Query: 423 PKGVLHTTGGYMVYTATTFKYAFDYKPSDVYWCTADCGWITGHSYVTYGPLLNGASVVVY 482
           PKGVLHTTGGYMVYTATTFKYAFDYKP DVYWCTADCGWITGHSYVTYGPLLNGASVVVY
Sbjct: 424 PKGVLHTTGGYMVYTATTFKYAFDYKPCDVYWCTADCGWITGHSYVTYGPLLNGASVVVY 483

Query: 483 EGAPNYPDPGRCWDIVDKYRVTIFYTAPTLVRALMREGNEHVTRYSRKSLRVLGSVGEPI 542
           EGAPNYPDPGRCWDIVDK++VTIFYTAPTLVR+LMREGNEHVTRYSRKSLRVLGSVGEPI
Sbjct: 484 EGAPNYPDPGRCWDIVDKHKVTIFYTAPTLVRSLMREGNEHVTRYSRKSLRVLGSVGEPI 543

Query: 543 NPSAWRWFFNVVGESRCPISDTWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVLVD 602
           NPSAWRWFFNVVGESRCPISDTWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVLVD
Sbjct: 544 NPSAWRWFFNVVGESRCPISDTWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVLVD 603

Query: 603 EKGNEIEGECSGYLCLKSSWPAAFRTLYNDQERYETTYFKPFPGYYFTGDGCTRDRDGYY 662
           EKGNEIEGECSGYLCLKSSWPAAFRTLYNDQERYE TYFKPFPGYYFTGDGCTRD+DGYY
Sbjct: 604 EKGNEIEGECSGYLCLKSSWPAAFRTLYNDQERYEMTYFKPFPGYYFTGDGCTRDKDGYY 663

Query: 663 WLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGIEHEVKGQGIYAFVTLVDGVS 722
           WLTGRVDDVINVSGHRIGTAEVESALVSHP CAEAAVVGIEHEVKGQGIYAFVTLVDGVS
Sbjct: 664 WLTGRVDDVINVSGHRIGTAEVESALVSHPHCAEAAVVGIEHEVKGQGIYAFVTLVDGVS 723

Query: 723 YSEELRKSLILTVRKQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRKIASKQLNELGDTS 782
           YSEELRKSLILTVRKQIGAFAAPD+IHWAPGLPKTRSGKIMRRILRKIASKQL+ELGDTS
Sbjct: 724 YSEELRKSLILTVRKQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASKQLDELGDTS 783

Query: 783 TLADPGVVDQLIELADC 799
           TLADPGVVDQLIELADC
Sbjct: 784 TLADPGVVDQLIELADC 790

BLAST of Sgr011532 vs. NCBI nr
Match: XP_022148218.1 (acetyl-coenzyme A synthetase, chloroplastic/glyoxysomal isoform X2 [Momordica charantia])

HSP 1 Score: 1417.9 bits (3669), Expect = 0.0e+00
Identity = 677/706 (95.89%), Postives = 691/706 (97.88%), Query Frame = 0

Query: 93  SRTSGEQDKVAMGFTDVTASDRLTHLDSSAARKIPRLNATVLGESLASEDDQLILPSDHF 152
           SRTSGEQ +V MGFTD+TA DRLT +DSSAARKIPRLNATVLGESLASEDDQLILPSD F
Sbjct: 5   SRTSGEQAQVVMGFTDLTAPDRLTQVDSSAARKIPRLNATVLGESLASEDDQLILPSDQF 64

Query: 153 SSRAHVSSHHKYLEMYKRSIEDPAGFWSDIASSEFFWKHTWDQQVYSENLDVRKGQIKIE 212
           SSRAHVSSH KYLEMYKRSIEDPAGFWSDIASSEFFWKH WDQ VYSENLDVRKG+IKIE
Sbjct: 65  SSRAHVSSHQKYLEMYKRSIEDPAGFWSDIASSEFFWKHRWDQLVYSENLDVRKGEIKIE 124

Query: 213 WFKGGITNICYNCLDRNIEAGLGEKVALYWEGNEPCFDGTLTYTQLLHKVCQLANFLKDN 272
           WFKGGITNICYNCLDRNIEAGLG+KVALYWEGNEP FDGTLTYT+LLHKVCQLAN+LKDN
Sbjct: 125 WFKGGITNICYNCLDRNIEAGLGDKVALYWEGNEPGFDGTLTYTELLHKVCQLANYLKDN 184

Query: 273 GVRKGDAVVIYMPMLMELPIAMLACARIGAIHSVVFAGFSSESLAQRIIDCKPKIVITCN 332
           GV+KGDAVVIYMPMLMELPIAMLACARIGAIHSVVFAGFSSESLAQRIIDCKPKIVITCN
Sbjct: 185 GVKKGDAVVIYMPMLMELPIAMLACARIGAIHSVVFAGFSSESLAQRIIDCKPKIVITCN 244

Query: 333 AVKRGSKVIHLKDIVDAALTESAQNEVSVATCLTYENESALKSESTKWKEGRDIWWQDVV 392
           AVKRGSKVIHLKDIVDAALTESAQNEVSVATCLTYENESALK EST WKEGRDIWWQDVV
Sbjct: 245 AVKRGSKVIHLKDIVDAALTESAQNEVSVATCLTYENESALKRESTTWKEGRDIWWQDVV 304

Query: 393 PTYPTTCTVEWVDAEHPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPSDVY 452
           PTYPTTCTVEWVDAE PLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKP DVY
Sbjct: 305 PTYPTTCTVEWVDAEFPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPCDVY 364

Query: 453 WCTADCGWITGHSYVTYGPLLNGASVVVYEGAPNYPDPGRCWDIVDKYRVTIFYTAPTLV 512
           WCTADCGWITGHSYVTYGPLLNGASVVVYEGAPNYPDPGRCWDIVDK++VTIFYTAPTLV
Sbjct: 365 WCTADCGWITGHSYVTYGPLLNGASVVVYEGAPNYPDPGRCWDIVDKHKVTIFYTAPTLV 424

Query: 513 RALMREGNEHVTRYSRKSLRVLGSVGEPINPSAWRWFFNVVGESRCPISDTWWQTETGGF 572
           R+LMREGNEHVTRYSRKSLRVLGSVGEPINPSAWRWFFNVVGESRCPISDTWWQTETGGF
Sbjct: 425 RSLMREGNEHVTRYSRKSLRVLGSVGEPINPSAWRWFFNVVGESRCPISDTWWQTETGGF 484

Query: 573 MITPLPGAWPQKPGSATFPFFGVQPVLVDEKGNEIEGECSGYLCLKSSWPAAFRTLYNDQ 632
           MITPLPGAWPQKPGSATFPFFGVQPVLVDEKGNEIEGECSGYLCLKSSWPAAFRTLYNDQ
Sbjct: 485 MITPLPGAWPQKPGSATFPFFGVQPVLVDEKGNEIEGECSGYLCLKSSWPAAFRTLYNDQ 544

Query: 633 ERYETTYFKPFPGYYFTGDGCTRDRDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQ 692
           ERYE TYFKPFPGYYFTGDGCTRD+DGYYWLTGRVDDVINVSGHRIGTAEVESALVSHP 
Sbjct: 545 ERYEMTYFKPFPGYYFTGDGCTRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPH 604

Query: 693 CAEAAVVGIEHEVKGQGIYAFVTLVDGVSYSEELRKSLILTVRKQIGAFAAPDKIHWAPG 752
           CAEAAVVGIEHEVKGQGIYAFVTLVDGVSYSEELRKSLILTVRKQIGAFAAPD+IHWAPG
Sbjct: 605 CAEAAVVGIEHEVKGQGIYAFVTLVDGVSYSEELRKSLILTVRKQIGAFAAPDRIHWAPG 664

Query: 753 LPKTRSGKIMRRILRKIASKQLNELGDTSTLADPGVVDQLIELADC 799
           LPKTRSGKIMRRILRKIASKQL+ELGDTSTLADPGVVDQLIELADC
Sbjct: 665 LPKTRSGKIMRRILRKIASKQLDELGDTSTLADPGVVDQLIELADC 710

BLAST of Sgr011532 vs. NCBI nr
Match: XP_022148219.1 (acetyl-coenzyme A synthetase, chloroplastic/glyoxysomal isoform X3 [Momordica charantia])

HSP 1 Score: 1416.7 bits (3666), Expect = 0.0e+00
Identity = 676/705 (95.89%), Postives = 690/705 (97.87%), Query Frame = 0

Query: 94  RTSGEQDKVAMGFTDVTASDRLTHLDSSAARKIPRLNATVLGESLASEDDQLILPSDHFS 153
           RTSGEQ +V MGFTD+TA DRLT +DSSAARKIPRLNATVLGESLASEDDQLILPSD FS
Sbjct: 5   RTSGEQAQVVMGFTDLTAPDRLTQVDSSAARKIPRLNATVLGESLASEDDQLILPSDQFS 64

Query: 154 SRAHVSSHHKYLEMYKRSIEDPAGFWSDIASSEFFWKHTWDQQVYSENLDVRKGQIKIEW 213
           SRAHVSSH KYLEMYKRSIEDPAGFWSDIASSEFFWKH WDQ VYSENLDVRKG+IKIEW
Sbjct: 65  SRAHVSSHQKYLEMYKRSIEDPAGFWSDIASSEFFWKHRWDQLVYSENLDVRKGEIKIEW 124

Query: 214 FKGGITNICYNCLDRNIEAGLGEKVALYWEGNEPCFDGTLTYTQLLHKVCQLANFLKDNG 273
           FKGGITNICYNCLDRNIEAGLG+KVALYWEGNEP FDGTLTYT+LLHKVCQLAN+LKDNG
Sbjct: 125 FKGGITNICYNCLDRNIEAGLGDKVALYWEGNEPGFDGTLTYTELLHKVCQLANYLKDNG 184

Query: 274 VRKGDAVVIYMPMLMELPIAMLACARIGAIHSVVFAGFSSESLAQRIIDCKPKIVITCNA 333
           V+KGDAVVIYMPMLMELPIAMLACARIGAIHSVVFAGFSSESLAQRIIDCKPKIVITCNA
Sbjct: 185 VKKGDAVVIYMPMLMELPIAMLACARIGAIHSVVFAGFSSESLAQRIIDCKPKIVITCNA 244

Query: 334 VKRGSKVIHLKDIVDAALTESAQNEVSVATCLTYENESALKSESTKWKEGRDIWWQDVVP 393
           VKRGSKVIHLKDIVDAALTESAQNEVSVATCLTYENESALK EST WKEGRDIWWQDVVP
Sbjct: 245 VKRGSKVIHLKDIVDAALTESAQNEVSVATCLTYENESALKRESTTWKEGRDIWWQDVVP 304

Query: 394 TYPTTCTVEWVDAEHPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPSDVYW 453
           TYPTTCTVEWVDAE PLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKP DVYW
Sbjct: 305 TYPTTCTVEWVDAEFPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPCDVYW 364

Query: 454 CTADCGWITGHSYVTYGPLLNGASVVVYEGAPNYPDPGRCWDIVDKYRVTIFYTAPTLVR 513
           CTADCGWITGHSYVTYGPLLNGASVVVYEGAPNYPDPGRCWDIVDK++VTIFYTAPTLVR
Sbjct: 365 CTADCGWITGHSYVTYGPLLNGASVVVYEGAPNYPDPGRCWDIVDKHKVTIFYTAPTLVR 424

Query: 514 ALMREGNEHVTRYSRKSLRVLGSVGEPINPSAWRWFFNVVGESRCPISDTWWQTETGGFM 573
           +LMREGNEHVTRYSRKSLRVLGSVGEPINPSAWRWFFNVVGESRCPISDTWWQTETGGFM
Sbjct: 425 SLMREGNEHVTRYSRKSLRVLGSVGEPINPSAWRWFFNVVGESRCPISDTWWQTETGGFM 484

Query: 574 ITPLPGAWPQKPGSATFPFFGVQPVLVDEKGNEIEGECSGYLCLKSSWPAAFRTLYNDQE 633
           ITPLPGAWPQKPGSATFPFFGVQPVLVDEKGNEIEGECSGYLCLKSSWPAAFRTLYNDQE
Sbjct: 485 ITPLPGAWPQKPGSATFPFFGVQPVLVDEKGNEIEGECSGYLCLKSSWPAAFRTLYNDQE 544

Query: 634 RYETTYFKPFPGYYFTGDGCTRDRDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQC 693
           RYE TYFKPFPGYYFTGDGCTRD+DGYYWLTGRVDDVINVSGHRIGTAEVESALVSHP C
Sbjct: 545 RYEMTYFKPFPGYYFTGDGCTRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPHC 604

Query: 694 AEAAVVGIEHEVKGQGIYAFVTLVDGVSYSEELRKSLILTVRKQIGAFAAPDKIHWAPGL 753
           AEAAVVGIEHEVKGQGIYAFVTLVDGVSYSEELRKSLILTVRKQIGAFAAPD+IHWAPGL
Sbjct: 605 AEAAVVGIEHEVKGQGIYAFVTLVDGVSYSEELRKSLILTVRKQIGAFAAPDRIHWAPGL 664

Query: 754 PKTRSGKIMRRILRKIASKQLNELGDTSTLADPGVVDQLIELADC 799
           PKTRSGKIMRRILRKIASKQL+ELGDTSTLADPGVVDQLIELADC
Sbjct: 665 PKTRSGKIMRRILRKIASKQLDELGDTSTLADPGVVDQLIELADC 709

BLAST of Sgr011532 vs. NCBI nr
Match: XP_038899318.1 (acetyl-coenzyme A synthetase, chloroplastic/glyoxysomal isoform X1 [Benincasa hispida])

HSP 1 Score: 1407.1 bits (3641), Expect = 0.0e+00
Identity = 700/823 (85.05%), Postives = 739/823 (89.79%), Query Frame = 0

Query: 1   MLN---TVATSSYGRPFPASFNPTLIIFPSAAIPL-PNNSNPKIHNRKQLNYNQRHGHVN 60
           MLN    +++ +  RPFPAS  P  I+FPSAA  +  +N NP + N K+        H+N
Sbjct: 1   MLNPPIVISSCNAARPFPASSIPG-ILFPSAAAAIKSDNPNPNLRNCKE-------RHIN 60

Query: 61  TFHNYYYLN--------SCL--PPSCCARSFICSSWTSASGFCCSLSRT---------SG 120
           +FHNY Y N        SCL  PPS  A SF+ S   S SGF  SL+RT          G
Sbjct: 61  SFHNYLYFNITSTATSSSCLLRPPS--AGSFLSS---SLSGFSSSLNRTRGGDGDGDGDG 120

Query: 121 EQDK-VAM-GFTDVTASDRLTHLDSSAARKIPRLNATVLGESLASEDDQLILPSDHFSSR 180
            +DK ++M GFTDV A      LDSSAARKIPRLNA VLGESLA+EDDQLIL SD FSS 
Sbjct: 121 RRDKNLSMGGFTDVAA------LDSSAARKIPRLNAAVLGESLATEDDQLILSSDQFSSN 180

Query: 181 AHVSSHHKYLEMYKRSIEDPAGFWSDIASSEFFWKHTWDQQVYSENLDVRKGQIKIEWFK 240
           AH+SS+H+YLEMYKRSIEDPAGFWSDIASSEFFWK  WDQQVYSENLDVRKG+IKIEWFK
Sbjct: 181 AHISSYHQYLEMYKRSIEDPAGFWSDIASSEFFWKQKWDQQVYSENLDVRKGEIKIEWFK 240

Query: 241 GGITNICYNCLDRNIEAGLGEKVALYWEGNEPCFDGTLTYTQLLHKVCQLANFLKDNGVR 300
           GGITNICYNCLDRNIEAGLG+KVA+YWEGNEP FDGTLTY +LLHKVCQLANFLKDNGVR
Sbjct: 241 GGITNICYNCLDRNIEAGLGDKVAIYWEGNEPGFDGTLTYIELLHKVCQLANFLKDNGVR 300

Query: 301 KGDAVVIYMPMLMELPIAMLACARIGAIHSVVFAGFSSESLAQRIIDCKPKIVITCNAVK 360
           KGDAVVIYMPMLMELPIAMLACARIGA+HSVVFAGFSSESLAQRIIDCKPKIVITCNAVK
Sbjct: 301 KGDAVVIYMPMLMELPIAMLACARIGAVHSVVFAGFSSESLAQRIIDCKPKIVITCNAVK 360

Query: 361 RGSKVIHLKDIVDAALTESAQNEVSVATCLTYENESALKSESTKWKEGRDIWWQDVVPTY 420
           RGSKVI+LKDI+DAAL ESAQN VSVATCLTYEN+SALK ESTKWKEGRDIWWQDVVPTY
Sbjct: 361 RGSKVIYLKDIIDAALIESAQNAVSVATCLTYENKSALKRESTKWKEGRDIWWQDVVPTY 420

Query: 421 PTTCTVEWVDAEHPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPSDVYWCT 480
           PTTCTVEWVDAE+PLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPSDVYWCT
Sbjct: 421 PTTCTVEWVDAEYPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPSDVYWCT 480

Query: 481 ADCGWITGHSYVTYGPLLNGASVVVYEGAPNYPDPGRCWDIVDKYRVTIFYTAPTLVRAL 540
           ADCGWITGHSYVTYGPLLNGASV+V+EGAPNYPDPGRCWDIVDKY+VTIFYTAPTLVR+L
Sbjct: 481 ADCGWITGHSYVTYGPLLNGASVIVFEGAPNYPDPGRCWDIVDKYKVTIFYTAPTLVRSL 540

Query: 541 MREGNEHVTRYSRKSLRVLGSVGEPINPSAWRWFFNVVGESRCPISDTWWQTETGGFMIT 600
           MREGNEHVTRY RKSLRVLGSVGEPINPSAWRWF+NVVGESRCPISDTWWQTETGGFMIT
Sbjct: 541 MREGNEHVTRYLRKSLRVLGSVGEPINPSAWRWFYNVVGESRCPISDTWWQTETGGFMIT 600

Query: 601 PLPGAWPQKPGSATFPFFGVQPVLVDEKGNEIEGECSGYLCLKSSWPAAFRTLYNDQERY 660
           PLPGAW QKPGSATFPFFGVQPVLVDEKGNEIEGECSGYLCLKSSWP+AFRTLYNDQERY
Sbjct: 601 PLPGAWSQKPGSATFPFFGVQPVLVDEKGNEIEGECSGYLCLKSSWPSAFRTLYNDQERY 660

Query: 661 ETTYFKPFPGYYFTGDGCTRDRDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAE 720
           ETTYFKPFPGYYF+GDGCTRD+DGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAE
Sbjct: 661 ETTYFKPFPGYYFSGDGCTRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAE 720

Query: 721 AAVVGIEHEVKGQGIYAFVTLVDGVSYSEELRKSLILTVRKQIGAFAAPDKIHWAPGLPK 780
           AAVVG+EHEVKGQGIYAFVTLVDGVSYSEELRKSLI+TVRKQIGAFAAPDKIHWAPGLPK
Sbjct: 721 AAVVGVEHEVKGQGIYAFVTLVDGVSYSEELRKSLIITVRKQIGAFAAPDKIHWAPGLPK 780

Query: 781 TRSGKIMRRILRKIASKQLNELGDTSTLADPGVVDQLIELADC 799
           TRSGKIMRRILRKIASKQL ELGD STLADPGVVDQLIELADC
Sbjct: 781 TRSGKIMRRILRKIASKQLEELGDISTLADPGVVDQLIELADC 804

BLAST of Sgr011532 vs. NCBI nr
Match: XP_022148220.1 (acetyl-coenzyme A synthetase, chloroplastic/glyoxysomal isoform X4 [Momordica charantia] >XP_022148222.1 acetyl-coenzyme A synthetase, chloroplastic/glyoxysomal isoform X4 [Momordica charantia] >XP_022148223.1 acetyl-coenzyme A synthetase, chloroplastic/glyoxysomal isoform X4 [Momordica charantia])

HSP 1 Score: 1404.0 bits (3633), Expect = 0.0e+00
Identity = 669/695 (96.26%), Postives = 682/695 (98.13%), Query Frame = 0

Query: 104 MGFTDVTASDRLTHLDSSAARKIPRLNATVLGESLASEDDQLILPSDHFSSRAHVSSHHK 163
           MGFTD+TA DRLT +DSSAARKIPRLNATVLGESLASEDDQLILPSD FSSRAHVSSH K
Sbjct: 1   MGFTDLTAPDRLTQVDSSAARKIPRLNATVLGESLASEDDQLILPSDQFSSRAHVSSHQK 60

Query: 164 YLEMYKRSIEDPAGFWSDIASSEFFWKHTWDQQVYSENLDVRKGQIKIEWFKGGITNICY 223
           YLEMYKRSIEDPAGFWSDIASSEFFWKH WDQ VYSENLDVRKG+IKIEWFKGGITNICY
Sbjct: 61  YLEMYKRSIEDPAGFWSDIASSEFFWKHRWDQLVYSENLDVRKGEIKIEWFKGGITNICY 120

Query: 224 NCLDRNIEAGLGEKVALYWEGNEPCFDGTLTYTQLLHKVCQLANFLKDNGVRKGDAVVIY 283
           NCLDRNIEAGLG+KVALYWEGNEP FDGTLTYT+LLHKVCQLAN+LKDNGV+KGDAVVIY
Sbjct: 121 NCLDRNIEAGLGDKVALYWEGNEPGFDGTLTYTELLHKVCQLANYLKDNGVKKGDAVVIY 180

Query: 284 MPMLMELPIAMLACARIGAIHSVVFAGFSSESLAQRIIDCKPKIVITCNAVKRGSKVIHL 343
           MPMLMELPIAMLACARIGAIHSVVFAGFSSESLAQRIIDCKPKIVITCNAVKRGSKVIHL
Sbjct: 181 MPMLMELPIAMLACARIGAIHSVVFAGFSSESLAQRIIDCKPKIVITCNAVKRGSKVIHL 240

Query: 344 KDIVDAALTESAQNEVSVATCLTYENESALKSESTKWKEGRDIWWQDVVPTYPTTCTVEW 403
           KDIVDAALTESAQNEVSVATCLTYENESALK EST WKEGRDIWWQDVVPTYPTTCTVEW
Sbjct: 241 KDIVDAALTESAQNEVSVATCLTYENESALKRESTTWKEGRDIWWQDVVPTYPTTCTVEW 300

Query: 404 VDAEHPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPSDVYWCTADCGWITG 463
           VDAE PLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKP DVYWCTADCGWITG
Sbjct: 301 VDAEFPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPCDVYWCTADCGWITG 360

Query: 464 HSYVTYGPLLNGASVVVYEGAPNYPDPGRCWDIVDKYRVTIFYTAPTLVRALMREGNEHV 523
           HSYVTYGPLLNGASVVVYEGAPNYPDPGRCWDIVDK++VTIFYTAPTLVR+LMREGNEHV
Sbjct: 361 HSYVTYGPLLNGASVVVYEGAPNYPDPGRCWDIVDKHKVTIFYTAPTLVRSLMREGNEHV 420

Query: 524 TRYSRKSLRVLGSVGEPINPSAWRWFFNVVGESRCPISDTWWQTETGGFMITPLPGAWPQ 583
           TRYSRKSLRVLGSVGEPINPSAWRWFFNVVGESRCPISDTWWQTETGGFMITPLPGAWPQ
Sbjct: 421 TRYSRKSLRVLGSVGEPINPSAWRWFFNVVGESRCPISDTWWQTETGGFMITPLPGAWPQ 480

Query: 584 KPGSATFPFFGVQPVLVDEKGNEIEGECSGYLCLKSSWPAAFRTLYNDQERYETTYFKPF 643
           KPGSATFPFFGVQPVLVDEKGNEIEGECSGYLCLKSSWPAAFRTLYNDQERYE TYFKPF
Sbjct: 481 KPGSATFPFFGVQPVLVDEKGNEIEGECSGYLCLKSSWPAAFRTLYNDQERYEMTYFKPF 540

Query: 644 PGYYFTGDGCTRDRDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGIEH 703
           PGYYFTGDGCTRD+DGYYWLTGRVDDVINVSGHRIGTAEVESALVSHP CAEAAVVGIEH
Sbjct: 541 PGYYFTGDGCTRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPHCAEAAVVGIEH 600

Query: 704 EVKGQGIYAFVTLVDGVSYSEELRKSLILTVRKQIGAFAAPDKIHWAPGLPKTRSGKIMR 763
           EVKGQGIYAFVTLVDGVSYSEELRKSLILTVRKQIGAFAAPD+IHWAPGLPKTRSGKIMR
Sbjct: 601 EVKGQGIYAFVTLVDGVSYSEELRKSLILTVRKQIGAFAAPDRIHWAPGLPKTRSGKIMR 660

Query: 764 RILRKIASKQLNELGDTSTLADPGVVDQLIELADC 799
           RILRKIASKQL+ELGDTSTLADPGVVDQLIELADC
Sbjct: 661 RILRKIASKQLDELGDTSTLADPGVVDQLIELADC 695

BLAST of Sgr011532 vs. ExPASy Swiss-Prot
Match: B9DGD6 (Acetyl-coenzyme A synthetase, chloroplastic/glyoxysomal OS=Arabidopsis thaliana OX=3702 GN=ACS PE=1 SV=1)

HSP 1 Score: 1193.7 bits (3087), Expect = 0.0e+00
Identity = 556/692 (80.35%), Postives = 626/692 (90.46%), Query Frame = 0

Query: 111 ASDRLTHLDS-----SAARKIPRLNATVLGESLASEDDQLILPSDHFSSRAHVSSHHKYL 170
           +S+ L H++S     S A KI +LNA VLGESLASE++ L+ PS  FS +A VSS  +Y+
Sbjct: 52  SSNSLRHVESMSQLPSGAGKISQLNAVVLGESLASEENDLVFPSKEFSGQALVSSPQQYM 111

Query: 171 EMYKRSIEDPAGFWSDIASSEFFWKHTWDQQVYSENLDVRKGQIKIEWFKGGITNICYNC 230
           EM+KRS++DPA FWSDIA SEF+WK  W  QV+SENLDVRKG I IEWFKGGITNICYNC
Sbjct: 112 EMHKRSMDDPAAFWSDIA-SEFYWKQKWGDQVFSENLDVRKGPISIEWFKGGITNICYNC 171

Query: 231 LDRNIEAGLGEKVALYWEGNEPCFDGTLTYTQLLHKVCQLANFLKDNGVRKGDAVVIYMP 290
           LD+N+EAGLG+K A++WEGNE   D +LTY++LL +VCQLAN+LKDNGV+KGDAVVIY+P
Sbjct: 172 LDKNVEAGLGDKTAIHWEGNELGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLP 231

Query: 291 MLMELPIAMLACARIGAIHSVVFAGFSSESLAQRIIDCKPKIVITCNAVKRGSKVIHLKD 350
           MLMELPIAMLACARIGA+HSVVFAGFS++SLAQRI+DCKP +++TCNAVKRG K I+LK 
Sbjct: 232 MLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILTCNAVKRGPKTINLKA 291

Query: 351 IVDAALTESAQNEVSVATCLTYENESALKSESTKWKEGRDIWWQDVVPTYPTTCTVEWVD 410
           IVDAAL +S+++ VSV  CLTY+N  A   E+TKW+ GRD+WWQDV+  YPT+C VEWVD
Sbjct: 292 IVDAALDQSSKDGVSVGICLTYDNSLATTRENTKWQNGRDVWWQDVISQYPTSCEVEWVD 351

Query: 411 AEHPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPSDVYWCTADCGWITGHS 470
           AE PLFLLYTSGSTGKPKGVLHTTGGYM+YTATTFKYAFDYK +DVYWCTADCGWITGHS
Sbjct: 352 AEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKYAFDYKSTDVYWCTADCGWITGHS 411

Query: 471 YVTYGPLLNGASVVVYEGAPNYPDPGRCWDIVDKYRVTIFYTAPTLVRALMREGNEHVTR 530
           YVTYGP+LNGA+VVV+EGAPNYPDPGRCWDIVDKY+V+IFYTAPTLVR+LMR+ ++ VTR
Sbjct: 412 YVTYGPMLNGATVVVFEGAPNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTR 471

Query: 531 YSRKSLRVLGSVGEPINPSAWRWFFNVVGESRCPISDTWWQTETGGFMITPLPGAWPQKP 590
           +SRKSLRVLGSVGEPINPSAWRWFFNVVG+SRCPISDTWWQTETGGFMITPLPGAWPQKP
Sbjct: 472 HSRKSLRVLGSVGEPINPSAWRWFFNVVGDSRCPISDTWWQTETGGFMITPLPGAWPQKP 531

Query: 591 GSATFPFFGVQPVLVDEKGNEIEGECSGYLCLKSSWPAAFRTLYNDQERYETTYFKPFPG 650
           GSATFPFFGVQPV+VDEKGNEIEGECSGYLC+K SWP AFRTL+ D ERYETTYFKPF G
Sbjct: 532 GSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGSWPGAFRTLFGDHERYETTYFKPFAG 591

Query: 651 YYFTGDGCTRDRDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGIEHEV 710
           YYF+GDGC+RD+DGYYWLTGRVDDVINVSGHRIGTAEVESALV HPQCAEAAVVGIEHEV
Sbjct: 592 YYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEV 651

Query: 711 KGQGIYAFVTLVDGVSYSEELRKSLILTVRKQIGAFAAPDKIHWAPGLPKTRSGKIMRRI 770
           KGQGIYAFVTL++GV YSEELRKSL+L VR QIGAFAAPD+IHWAPGLPKTRSGKIMRRI
Sbjct: 652 KGQGIYAFVTLLEGVPYSEELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRI 711

Query: 771 LRKIASKQLNELGDTSTLADPGVVDQLIELAD 798
           LRKIAS+QL ELGDTSTLADP VVDQLI LAD
Sbjct: 712 LRKIASRQLEELGDTSTLADPSVVDQLIALAD 742

BLAST of Sgr011532 vs. ExPASy Swiss-Prot
Match: Q9NR19 (Acetyl-coenzyme A synthetase, cytoplasmic OS=Homo sapiens OX=9606 GN=ACSS2 PE=1 SV=1)

HSP 1 Score: 805.1 bits (2078), Expect = 7.3e-232
Identity = 385/664 (57.98%), Postives = 473/664 (71.23%), Query Frame = 0

Query: 148 PSDHFSSRAHVSSHHKYLEMYKRSIEDPAGFWSDIASSEFFWKHTWDQQVYSENLDVRKG 207
           P    S  AHV S  +Y E+++RS+E+P  FW DIA  EF+WK          N DV KG
Sbjct: 31  PPPEVSRSAHVPSLQRYRELHRRSVEEPREFWGDIA-KEFYWKTPCPGPFLRYNFDVTKG 90

Query: 208 QIKIEWFKGGITNICYNCLDRNI-EAGLGEKVALYWEGNEPCFDGTLTYTQLLHKVCQLA 267
           +I IEW KG  TNICYN LDRN+ E  LG+KVA YWEGNEP     +TY QLL +VCQ +
Sbjct: 91  KIFIEWMKGATTNICYNVLDRNVHEKKLGDKVAFYWEGNEPGETTQITYHQLLVQVCQFS 150

Query: 268 NFLKDNGVRKGDAVVIYMPMLMELPIAMLACARIGAIHSVVFAGFSSESLAQRIIDCKPK 327
           N L+  G++KGD V IYMPM+ EL +AMLACARIGA+HS+VFAGFSSESL +RI+D    
Sbjct: 151 NVLRKQGIQKGDRVAIYMPMIPELVVAMLACARIGALHSIVFAGFSSESLCERILDSSCS 210

Query: 328 IVITCNAVKRGSKVIHLKDIVDAALTESAQNEVSVATCLTYEN---------ESALKSES 387
           ++IT +A  RG K+++LK++ D AL +  +    V  C+  ++         +S  +S  
Sbjct: 211 LLITTDAFYRGEKLVNLKELADEALQKCQEKGFPVRCCIVVKHLGRAELGMGDSTSQSPP 270

Query: 388 TK---------WKEGRDIWWQDVVPTYPTTCTVEWVDAEHPLFLLYTSGSTGKPKGVLHT 447
            K         W +G D+WW +++      C  EW DAE PLF+LYTSGSTGKPKGV+HT
Sbjct: 271 IKRSCPDVQISWNQGIDLWWHELMQEAGDECEPEWCDAEDPLFILYTSGSTGKPKGVVHT 330

Query: 448 TGGYMVYTATTFKYAFDYKPSDVYWCTADCGWITGHSYVTYGPLLNGASVVVYEGAPNYP 507
            GGYM+Y ATTFKY FD+   DV+WCTAD GWITGHSYVTYGPL NGA+ V++EG P YP
Sbjct: 331 VGGYMLYVATTFKYVFDFHAEDVFWCTADIGWITGHSYVTYGPLANGATSVLFEGIPTYP 390

Query: 508 DPGRCWDIVDKYRVTIFYTAPTLVRALMREGNEHVTRYSRKSLRVLGSVGEPINPSAWRW 567
           D  R W IVDKY+VT FYTAPT +R LM+ G+E VT++SR SL+VLG+VGEPINP AW W
Sbjct: 391 DVNRLWSIVDKYKVTKFYTAPTAIRLLMKFGDEPVTKHSRASLQVLGTVGEPINPEAWLW 450

Query: 568 FFNVVGESRCPISDTWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVLVDEKGNEIE 627
           +  VVG  RCPI DT+WQTETGG M+TPLPGA P KPGSATFPFFGV P +++E G E+E
Sbjct: 451 YHRVVGAQRCPIVDTFWQTETGGHMLTPLPGATPMKPGSATFPFFGVAPAILNESGEELE 510

Query: 628 GECSGYLCLKSSWPAAFRTLYNDQERYETTYFKPFPGYYFTGDGCTRDRDGYYWLTGRVD 687
           GE  GYL  K  WP   RT+Y + ER+ETTYFK FPGYY TGDGC RD+DGYYW+TGR+D
Sbjct: 511 GEAEGYLVFKQPWPGIMRTVYGNHERFETTYFKKFPGYYVTGDGCQRDQDGYYWITGRID 570

Query: 688 DVINVSGHRIGTAEVESALVSHPQCAEAAVVGIEHEVKGQGIYAFVTLVDGVSYSEELRK 747
           D++NVSGH + TAEVESALV H   AEAAVVG  H VKG+ +Y FVTL DG ++S +L +
Sbjct: 571 DMLNVSGHLLSTAEVESALVEHEAVAEAAVVGHPHPVKGECLYCFVTLCDGHTFSPKLTE 630

Query: 748 SLILTVRKQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRKIASKQLNELGDTSTLADPGV 793
            L   +R++IG  A PD I  APGLPKTRSGKIMRR+LRKIA    ++LGD ST+ADP V
Sbjct: 631 ELKKQIREKIGPIATPDYIQNAPGLPKTRSGKIMRRVLRKIAQND-HDLGDMSTVADPSV 690

BLAST of Sgr011532 vs. ExPASy Swiss-Prot
Match: Q9QXG4 (Acetyl-coenzyme A synthetase, cytoplasmic OS=Mus musculus OX=10090 GN=Acss2 PE=1 SV=2)

HSP 1 Score: 802.4 bits (2071), Expect = 4.8e-231
Identity = 380/664 (57.23%), Postives = 474/664 (71.39%), Query Frame = 0

Query: 148 PSDHFSSRAHVSSHHKYLEMYKRSIEDPAGFWSDIASSEFFWKHTWDQQVYSENLDVRKG 207
           P       AHV S  +Y E+++RS+E+P  FW +IA  EF+WK          N DV KG
Sbjct: 31  PPPEVRRSAHVPSLQRYRELHRRSVEEPREFWGNIA-KEFYWKTACPGPFLQYNFDVTKG 90

Query: 208 QIKIEWFKGGITNICYNCLDRNI-EAGLGEKVALYWEGNEPCFDGTLTYTQLLHKVCQLA 267
           +I  EW KG  TNICYN LDRN+ E  LG+KVA YWEGNEP     +TY +LL +VCQ +
Sbjct: 91  KIFTEWMKGATTNICYNVLDRNVHEKKLGDKVAFYWEGNEPGETTKITYRELLVQVCQFS 150

Query: 268 NFLKDNGVRKGDAVVIYMPMLMELPIAMLACARIGAIHSVVFAGFSSESLAQRIIDCKPK 327
           N L+  G++KGD V IYMPM++EL +AMLACAR+GA+HS+VFAGFS+ESL +RI+D    
Sbjct: 151 NVLRKQGIQKGDRVAIYMPMILELVVAMLACARLGALHSIVFAGFSAESLCERILDSSCC 210

Query: 328 IVITCNAVKRGSKVIHLKDIVDAALTESAQNEVSVATCLTYE---------NESALKSES 387
           ++IT +A  RG K+++LK++ D +L +  +    V  C+  +         N+S  +S  
Sbjct: 211 LLITTDAFYRGEKLVNLKELADESLEKCREKGFPVRCCIVVKHLGRAELGMNDSPSQSPP 270

Query: 388 TK---------WKEGRDIWWQDVVPTYPTTCTVEWVDAEHPLFLLYTSGSTGKPKGVLHT 447
            K         W EG D+WW +++      C  EW DAE PLF+LYTSGSTGKPKGV+HT
Sbjct: 271 VKRPCPDVQICWNEGVDLWWHELMQQAGDECEPEWCDAEDPLFILYTSGSTGKPKGVVHT 330

Query: 448 TGGYMVYTATTFKYAFDYKPSDVYWCTADCGWITGHSYVTYGPLLNGASVVVYEGAPNYP 507
            GGYM+Y ATTFKY FD+ P DV+WCTAD GWITGHSYVTYGPL NGA+ V++EG P YP
Sbjct: 331 IGGYMLYVATTFKYVFDFHPEDVFWCTADIGWITGHSYVTYGPLANGATSVLFEGIPTYP 390

Query: 508 DPGRCWDIVDKYRVTIFYTAPTLVRALMREGNEHVTRYSRKSLRVLGSVGEPINPSAWRW 567
           D GR W IVDKY+VT FYTAPT +R LM+ G++ VT++SR SL+VLG+VGEPINP AW W
Sbjct: 391 DEGRLWSIVDKYKVTKFYTAPTAIRMLMKFGDDPVTKHSRASLQVLGTVGEPINPEAWLW 450

Query: 568 FFNVVGESRCPISDTWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVLVDEKGNEIE 627
           +  VVG  RCPI DT+WQTETGG M+TPLPGA P KPGSA+FPFFGV P +++E G E+E
Sbjct: 451 YHRVVGSQRCPIVDTFWQTETGGHMLTPLPGATPMKPGSASFPFFGVAPAILNESGEELE 510

Query: 628 GECSGYLCLKSSWPAAFRTLYNDQERYETTYFKPFPGYYFTGDGCTRDRDGYYWLTGRVD 687
           GE  GYL  K  WP   RT+Y +  R+ETTYFK FPGYY TGDGC RD+DGYYW+TGR+D
Sbjct: 511 GEAEGYLVFKQPWPGIMRTVYGNHTRFETTYFKKFPGYYVTGDGCRRDQDGYYWITGRID 570

Query: 688 DVINVSGHRIGTAEVESALVSHPQCAEAAVVGIEHEVKGQGIYAFVTLVDGVSYSEELRK 747
           D++NVSGH + TAEVESALV H   AEAAVVG  H VKG+ +Y FVTL DG ++S  L +
Sbjct: 571 DMLNVSGHLLSTAEVESALVEHEAVAEAAVVGHPHPVKGECLYCFVTLCDGHTFSPTLTE 630

Query: 748 SLILTVRKQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRKIASKQLNELGDTSTLADPGV 793
            L   +R++IG  A PD I  APGLPKTRSGKIMRR+LRKIA    ++LGDTST+ADP V
Sbjct: 631 ELKKQIREKIGPIATPDYIQNAPGLPKTRSGKIMRRVLRKIAQND-HDLGDTSTVADPSV 690

BLAST of Sgr011532 vs. ExPASy Swiss-Prot
Match: Q04P35 (Acetyl-coenzyme A synthetase OS=Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197) OX=355277 GN=acsA PE=3 SV=1)

HSP 1 Score: 781.6 bits (2017), Expect = 8.7e-225
Identity = 381/656 (58.08%), Postives = 465/656 (70.88%), Query Frame = 0

Query: 143 DQLILPSDHFSSRAHVSSHHKYLEMYKRSIEDPAGFWSDIASSEFFWKHTWDQQVYSENL 202
           ++++LPS  F   ++++    Y  +YK SIE+P  FW+  A +   W   W + +   N 
Sbjct: 4   ERIVLPSAEFKKNSNITL-KDYKSLYKESIENPNKFWAKEA-NRLTWFKKWTKVL---NH 63

Query: 203 DVRKGQIKIEWFKGGITNICYNCLDRNIEAGLGEKVALYWEGNEPCFDGTLTYTQLLHKV 262
           D +    K+EWFKGG  N+ YNCLDR+I   L  K AL WEG+ P     LTY  +  +V
Sbjct: 64  DFKNA--KVEWFKGGKLNVSYNCLDRHISTPLKNKAALIWEGDNPSESKVLTYYDVYREV 123

Query: 263 CQLANFLKDNGVRKGDAVVIYMPMLMELPIAMLACARIGAIHSVVFAGFSSESLAQRIID 322
            + AN LK  GV+KGD V++Y+PM+ EL I +LAC RIGAIHSVVF GFS E+L  RI D
Sbjct: 124 NRFANVLKKYGVKKGDRVLVYLPMIPELAITILACTRIGAIHSVVFGGFSPEALQSRIDD 183

Query: 323 CKPKIVITCNAVKRGSKVIHLKDIVDAALTESAQNEVSVATCLTYENESALKSESTKWKE 382
           CKPK+++T +   RG K I LK  VD AL +S ++  +V       NES L      WK+
Sbjct: 184 CKPKLIVTADGGFRGGKPIELKRNVDIALEKSKEDVKTVIVVRRTGNESGL-----VWKD 243

Query: 383 GRDIWWQDVV--PTYPTTCTVEWVDAEHPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTF 442
           GRD W+  ++  P     C  E +DAE PLF+LYTSGSTGKPKGVLHTTGGY++    TF
Sbjct: 244 GRDYWYHFLIGDPDLSPYCKPESMDAEDPLFILYTSGSTGKPKGVLHTTGGYLLGVNLTF 303

Query: 443 KYAFDYKPSDVYWCTADCGWITGHSYVTYGPLLNGASVVVYEGAPNYPDPGRCWDIVDKY 502
            Y FD KP D YWCTAD GW+TGHSY+ YGPL NGAS V++EG P+YPD GR WD++DKY
Sbjct: 304 HYVFDIKPEDTYWCTADIGWVTGHSYLVYGPLSNGASSVMFEGVPSYPDAGRFWDVIDKY 363

Query: 503 RVTIFYTAPTLVRALMREGNEHVTRYSRKSLRVLGSVGEPINPSAWRWFFNVVGESRCPI 562
            V IFYTAPT +RALMREG  HV + +  SLR+LGSVGEPINP AW W+F ++G+ +CPI
Sbjct: 364 GVNIFYTAPTAIRALMREGLTHVQKRNLSSLRLLGSVGEPINPEAWEWYFKIIGKEKCPI 423

Query: 563 SDTWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVLVDEKGNEI--EGECSGYLCLK 622
            DTWWQTETG  MIT LPGA PQKPGSAT PFFGVQPVLVD  G EI  +GE SG LC+K
Sbjct: 424 VDTWWQTETGSIMITALPGAIPQKPGSATLPFFGVQPVLVDNDGKEINDKGEVSGNLCIK 483

Query: 623 SSWPAAFRTLYNDQERYETTYFKPFPGYYFTGDGCTRDRDGYYWLTGRVDDVINVSGHRI 682
           S WP+  R +Y D +R+  TYF  F GYYFTGDG  RD+DGYYW+TGRVDDVINVSGHRI
Sbjct: 484 SPWPSMMRGVYGDSKRFFDTYFSQFKGYYFTGDGARRDKDGYYWITGRVDDVINVSGHRI 543

Query: 683 GTAEVESALVSHPQCAEAAVVGIEHEVKGQGIYAFVTLVDGVSYSEELRKSLILTVRKQI 742
           G+AEVESALV +   AEAAVVG  H++KGQGIYA+VT+ +G++ ++ L+K L+  V K I
Sbjct: 544 GSAEVESALVENRSVAEAAVVGFPHDIKGQGIYAYVTVKEGIATNDTLKKELVAIVEKVI 603

Query: 743 GAFAAPDKIHWAPGLPKTRSGKIMRRILRKIASKQLNELGDTSTLADPGVVDQLIE 795
           G  A PD IHWAP LPKTRSGKIMRRILRKIAS +   LGDTSTLADP VV +LIE
Sbjct: 604 GKIARPDVIHWAPSLPKTRSGKIMRRILRKIASGEFEGLGDTSTLADPSVVQKLIE 647

BLAST of Sgr011532 vs. ExPASy Swiss-Prot
Match: Q056J9 (Acetyl-coenzyme A synthetase OS=Leptospira borgpetersenii serovar Hardjo-bovis (strain L550) OX=355276 GN=acsA PE=3 SV=1)

HSP 1 Score: 781.6 bits (2017), Expect = 8.7e-225
Identity = 381/656 (58.08%), Postives = 465/656 (70.88%), Query Frame = 0

Query: 143 DQLILPSDHFSSRAHVSSHHKYLEMYKRSIEDPAGFWSDIASSEFFWKHTWDQQVYSENL 202
           ++++LPS  F   ++++    Y  +YK SIE+P  FW+  A +   W   W + +   N 
Sbjct: 4   ERIVLPSAEFKKNSNITL-KDYKSLYKESIENPNKFWAKEA-NRLTWFKKWTKVL---NH 63

Query: 203 DVRKGQIKIEWFKGGITNICYNCLDRNIEAGLGEKVALYWEGNEPCFDGTLTYTQLLHKV 262
           D +    K+EWFKGG  N+ YNCLDR+I   L  K AL WEG+ P     LTY  +  +V
Sbjct: 64  DFKNA--KVEWFKGGKLNVSYNCLDRHISTPLKNKAALIWEGDNPSESKVLTYYDVYREV 123

Query: 263 CQLANFLKDNGVRKGDAVVIYMPMLMELPIAMLACARIGAIHSVVFAGFSSESLAQRIID 322
            + AN LK  GV+KGD V++Y+PM+ EL I +LAC RIGAIHSVVF GFS E+L  RI D
Sbjct: 124 NRFANVLKKYGVKKGDRVLVYLPMIPELAITILACTRIGAIHSVVFGGFSPEALQSRIDD 183

Query: 323 CKPKIVITCNAVKRGSKVIHLKDIVDAALTESAQNEVSVATCLTYENESALKSESTKWKE 382
           CKPK+++T +   RG K I LK  VD AL +S ++  +V       NES L      WK+
Sbjct: 184 CKPKLIVTADGGFRGGKPIELKRNVDIALEKSKEDVKTVIVVRRTGNESGL-----VWKD 243

Query: 383 GRDIWWQDVV--PTYPTTCTVEWVDAEHPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTF 442
           GRD W+  ++  P     C  E +DAE PLF+LYTSGSTGKPKGVLHTTGGY++    TF
Sbjct: 244 GRDYWYHFLISDPDLSPYCKPESMDAEDPLFILYTSGSTGKPKGVLHTTGGYLLGVNLTF 303

Query: 443 KYAFDYKPSDVYWCTADCGWITGHSYVTYGPLLNGASVVVYEGAPNYPDPGRCWDIVDKY 502
            Y FD KP D YWCTAD GW+TGHSY+ YGPL NGAS V++EG P+YPD GR WD++DKY
Sbjct: 304 HYVFDIKPEDTYWCTADIGWVTGHSYLVYGPLSNGASSVMFEGVPSYPDAGRFWDVIDKY 363

Query: 503 RVTIFYTAPTLVRALMREGNEHVTRYSRKSLRVLGSVGEPINPSAWRWFFNVVGESRCPI 562
            V IFYTAPT +RALMREG  HV + +  SLR+LGSVGEPINP AW W+F ++G+ +CPI
Sbjct: 364 GVNIFYTAPTAIRALMREGLTHVQKRNLSSLRLLGSVGEPINPEAWEWYFKIIGKEKCPI 423

Query: 563 SDTWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVLVDEKGNEI--EGECSGYLCLK 622
            DTWWQTETG  MIT LPGA PQKPGSAT PFFGVQPVLVD  G EI  +GE SG LC+K
Sbjct: 424 VDTWWQTETGSIMITALPGAIPQKPGSATLPFFGVQPVLVDNDGKEINDKGEVSGNLCIK 483

Query: 623 SSWPAAFRTLYNDQERYETTYFKPFPGYYFTGDGCTRDRDGYYWLTGRVDDVINVSGHRI 682
           S WP+  R +Y D +R+  TYF  F GYYFTGDG  RD+DGYYW+TGRVDDVINVSGHRI
Sbjct: 484 SPWPSMMRGVYGDSKRFFDTYFSQFKGYYFTGDGARRDKDGYYWITGRVDDVINVSGHRI 543

Query: 683 GTAEVESALVSHPQCAEAAVVGIEHEVKGQGIYAFVTLVDGVSYSEELRKSLILTVRKQI 742
           G+AEVESALV +   AEAAVVG  H++KGQGIYA+VT+ +G++ ++ L+K L+  V K I
Sbjct: 544 GSAEVESALVENRSVAEAAVVGFPHDIKGQGIYAYVTVKEGIATNDTLKKELVAIVEKVI 603

Query: 743 GAFAAPDKIHWAPGLPKTRSGKIMRRILRKIASKQLNELGDTSTLADPGVVDQLIE 795
           G  A PD IHWAP LPKTRSGKIMRRILRKIAS +   LGDTSTLADP VV +LIE
Sbjct: 604 GKIARPDVIHWAPSLPKTRSGKIMRRILRKIASGEFEGLGDTSTLADPSVVQKLIE 647

BLAST of Sgr011532 vs. ExPASy TrEMBL
Match: A0A6J1D4G7 (Acetate--CoA ligase OS=Momordica charantia OX=3673 GN=LOC111016935 PE=3 SV=1)

HSP 1 Score: 1492.6 bits (3863), Expect = 0.0e+00
Identity = 733/797 (91.97%), Postives = 754/797 (94.60%), Query Frame = 0

Query: 3   NTVATSS-YGRPFPASFNPTLIIFPSAAIPLPNNSNPKIHNRKQLNYNQRHGHVNTFHNY 62
           +TVATSS + RPFPA  NPT  +FPSA+    NNS  KIHN KQ NY +R   VNTFH+Y
Sbjct: 4   HTVATSSCFIRPFPA--NPT-FLFPSAS--SANNSVHKIHNWKQPNYKKR--QVNTFHSY 63

Query: 63  YYLNSCLPPSCCARSFICSSWTSASGFCCSLSRTSGEQDKVAMGFTDVTASDRLTHLDSS 122
           YY NSCL PS CARSF      S+SGFCCS SRTSGEQ +V MGFTD+TA DRLT +DSS
Sbjct: 64  YYFNSCLRPS-CARSF--GFTRSSSGFCCSFSRTSGEQAQVVMGFTDLTAPDRLTQVDSS 123

Query: 123 AARKIPRLNATVLGESLASEDDQLILPSDHFSSRAHVSSHHKYLEMYKRSIEDPAGFWSD 182
           AARKIPRLNATVLGESLASEDDQLILPSD FSSRAHVSSH KYLEMYKRSIEDPAGFWSD
Sbjct: 124 AARKIPRLNATVLGESLASEDDQLILPSDQFSSRAHVSSHQKYLEMYKRSIEDPAGFWSD 183

Query: 183 IASSEFFWKHTWDQQVYSENLDVRKGQIKIEWFKGGITNICYNCLDRNIEAGLGEKVALY 242
           IASSEFFWKH WDQ VYSENLDVRKG+IKIEWFKGGITNICYNCLDRNIEAGLG+KVALY
Sbjct: 184 IASSEFFWKHRWDQLVYSENLDVRKGEIKIEWFKGGITNICYNCLDRNIEAGLGDKVALY 243

Query: 243 WEGNEPCFDGTLTYTQLLHKVCQLANFLKDNGVRKGDAVVIYMPMLMELPIAMLACARIG 302
           WEGNEP FDGTLTYT+LLHKVCQLAN+LKDNGV+KGDAVVIYMPMLMELPIAMLACARIG
Sbjct: 244 WEGNEPGFDGTLTYTELLHKVCQLANYLKDNGVKKGDAVVIYMPMLMELPIAMLACARIG 303

Query: 303 AIHSVVFAGFSSESLAQRIIDCKPKIVITCNAVKRGSKVIHLKDIVDAALTESAQNEVSV 362
           AIHSVVFAGFSSESLAQRIIDCKPKIVITCNAVKRGSKVIHLKDIVDAALTESAQNEVSV
Sbjct: 304 AIHSVVFAGFSSESLAQRIIDCKPKIVITCNAVKRGSKVIHLKDIVDAALTESAQNEVSV 363

Query: 363 ATCLTYENESALKSESTKWKEGRDIWWQDVVPTYPTTCTVEWVDAEHPLFLLYTSGSTGK 422
           ATCLTYENESALK EST WKEGRDIWWQDVVPTYPTTCTVEWVDAE PLFLLYTSGSTGK
Sbjct: 364 ATCLTYENESALKRESTTWKEGRDIWWQDVVPTYPTTCTVEWVDAEFPLFLLYTSGSTGK 423

Query: 423 PKGVLHTTGGYMVYTATTFKYAFDYKPSDVYWCTADCGWITGHSYVTYGPLLNGASVVVY 482
           PKGVLHTTGGYMVYTATTFKYAFDYKP DVYWCTADCGWITGHSYVTYGPLLNGASVVVY
Sbjct: 424 PKGVLHTTGGYMVYTATTFKYAFDYKPCDVYWCTADCGWITGHSYVTYGPLLNGASVVVY 483

Query: 483 EGAPNYPDPGRCWDIVDKYRVTIFYTAPTLVRALMREGNEHVTRYSRKSLRVLGSVGEPI 542
           EGAPNYPDPGRCWDIVDK++VTIFYTAPTLVR+LMREGNEHVTRYSRKSLRVLGSVGEPI
Sbjct: 484 EGAPNYPDPGRCWDIVDKHKVTIFYTAPTLVRSLMREGNEHVTRYSRKSLRVLGSVGEPI 543

Query: 543 NPSAWRWFFNVVGESRCPISDTWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVLVD 602
           NPSAWRWFFNVVGESRCPISDTWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVLVD
Sbjct: 544 NPSAWRWFFNVVGESRCPISDTWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVLVD 603

Query: 603 EKGNEIEGECSGYLCLKSSWPAAFRTLYNDQERYETTYFKPFPGYYFTGDGCTRDRDGYY 662
           EKGNEIEGECSGYLCLKSSWPAAFRTLYNDQERYE TYFKPFPGYYFTGDGCTRD+DGYY
Sbjct: 604 EKGNEIEGECSGYLCLKSSWPAAFRTLYNDQERYEMTYFKPFPGYYFTGDGCTRDKDGYY 663

Query: 663 WLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGIEHEVKGQGIYAFVTLVDGVS 722
           WLTGRVDDVINVSGHRIGTAEVESALVSHP CAEAAVVGIEHEVKGQGIYAFVTLVDGVS
Sbjct: 664 WLTGRVDDVINVSGHRIGTAEVESALVSHPHCAEAAVVGIEHEVKGQGIYAFVTLVDGVS 723

Query: 723 YSEELRKSLILTVRKQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRKIASKQLNELGDTS 782
           YSEELRKSLILTVRKQIGAFAAPD+IHWAPGLPKTRSGKIMRRILRKIASKQL+ELGDTS
Sbjct: 724 YSEELRKSLILTVRKQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASKQLDELGDTS 783

Query: 783 TLADPGVVDQLIELADC 799
           TLADPGVVDQLIELADC
Sbjct: 784 TLADPGVVDQLIELADC 790

BLAST of Sgr011532 vs. ExPASy TrEMBL
Match: A0A6J1D3H3 (Acetate--CoA ligase OS=Momordica charantia OX=3673 GN=LOC111016935 PE=3 SV=1)

HSP 1 Score: 1417.9 bits (3669), Expect = 0.0e+00
Identity = 677/706 (95.89%), Postives = 691/706 (97.88%), Query Frame = 0

Query: 93  SRTSGEQDKVAMGFTDVTASDRLTHLDSSAARKIPRLNATVLGESLASEDDQLILPSDHF 152
           SRTSGEQ +V MGFTD+TA DRLT +DSSAARKIPRLNATVLGESLASEDDQLILPSD F
Sbjct: 5   SRTSGEQAQVVMGFTDLTAPDRLTQVDSSAARKIPRLNATVLGESLASEDDQLILPSDQF 64

Query: 153 SSRAHVSSHHKYLEMYKRSIEDPAGFWSDIASSEFFWKHTWDQQVYSENLDVRKGQIKIE 212
           SSRAHVSSH KYLEMYKRSIEDPAGFWSDIASSEFFWKH WDQ VYSENLDVRKG+IKIE
Sbjct: 65  SSRAHVSSHQKYLEMYKRSIEDPAGFWSDIASSEFFWKHRWDQLVYSENLDVRKGEIKIE 124

Query: 213 WFKGGITNICYNCLDRNIEAGLGEKVALYWEGNEPCFDGTLTYTQLLHKVCQLANFLKDN 272
           WFKGGITNICYNCLDRNIEAGLG+KVALYWEGNEP FDGTLTYT+LLHKVCQLAN+LKDN
Sbjct: 125 WFKGGITNICYNCLDRNIEAGLGDKVALYWEGNEPGFDGTLTYTELLHKVCQLANYLKDN 184

Query: 273 GVRKGDAVVIYMPMLMELPIAMLACARIGAIHSVVFAGFSSESLAQRIIDCKPKIVITCN 332
           GV+KGDAVVIYMPMLMELPIAMLACARIGAIHSVVFAGFSSESLAQRIIDCKPKIVITCN
Sbjct: 185 GVKKGDAVVIYMPMLMELPIAMLACARIGAIHSVVFAGFSSESLAQRIIDCKPKIVITCN 244

Query: 333 AVKRGSKVIHLKDIVDAALTESAQNEVSVATCLTYENESALKSESTKWKEGRDIWWQDVV 392
           AVKRGSKVIHLKDIVDAALTESAQNEVSVATCLTYENESALK EST WKEGRDIWWQDVV
Sbjct: 245 AVKRGSKVIHLKDIVDAALTESAQNEVSVATCLTYENESALKRESTTWKEGRDIWWQDVV 304

Query: 393 PTYPTTCTVEWVDAEHPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPSDVY 452
           PTYPTTCTVEWVDAE PLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKP DVY
Sbjct: 305 PTYPTTCTVEWVDAEFPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPCDVY 364

Query: 453 WCTADCGWITGHSYVTYGPLLNGASVVVYEGAPNYPDPGRCWDIVDKYRVTIFYTAPTLV 512
           WCTADCGWITGHSYVTYGPLLNGASVVVYEGAPNYPDPGRCWDIVDK++VTIFYTAPTLV
Sbjct: 365 WCTADCGWITGHSYVTYGPLLNGASVVVYEGAPNYPDPGRCWDIVDKHKVTIFYTAPTLV 424

Query: 513 RALMREGNEHVTRYSRKSLRVLGSVGEPINPSAWRWFFNVVGESRCPISDTWWQTETGGF 572
           R+LMREGNEHVTRYSRKSLRVLGSVGEPINPSAWRWFFNVVGESRCPISDTWWQTETGGF
Sbjct: 425 RSLMREGNEHVTRYSRKSLRVLGSVGEPINPSAWRWFFNVVGESRCPISDTWWQTETGGF 484

Query: 573 MITPLPGAWPQKPGSATFPFFGVQPVLVDEKGNEIEGECSGYLCLKSSWPAAFRTLYNDQ 632
           MITPLPGAWPQKPGSATFPFFGVQPVLVDEKGNEIEGECSGYLCLKSSWPAAFRTLYNDQ
Sbjct: 485 MITPLPGAWPQKPGSATFPFFGVQPVLVDEKGNEIEGECSGYLCLKSSWPAAFRTLYNDQ 544

Query: 633 ERYETTYFKPFPGYYFTGDGCTRDRDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQ 692
           ERYE TYFKPFPGYYFTGDGCTRD+DGYYWLTGRVDDVINVSGHRIGTAEVESALVSHP 
Sbjct: 545 ERYEMTYFKPFPGYYFTGDGCTRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPH 604

Query: 693 CAEAAVVGIEHEVKGQGIYAFVTLVDGVSYSEELRKSLILTVRKQIGAFAAPDKIHWAPG 752
           CAEAAVVGIEHEVKGQGIYAFVTLVDGVSYSEELRKSLILTVRKQIGAFAAPD+IHWAPG
Sbjct: 605 CAEAAVVGIEHEVKGQGIYAFVTLVDGVSYSEELRKSLILTVRKQIGAFAAPDRIHWAPG 664

Query: 753 LPKTRSGKIMRRILRKIASKQLNELGDTSTLADPGVVDQLIELADC 799
           LPKTRSGKIMRRILRKIASKQL+ELGDTSTLADPGVVDQLIELADC
Sbjct: 665 LPKTRSGKIMRRILRKIASKQLDELGDTSTLADPGVVDQLIELADC 710

BLAST of Sgr011532 vs. ExPASy TrEMBL
Match: A0A6J1D3C3 (Acetate--CoA ligase OS=Momordica charantia OX=3673 GN=LOC111016935 PE=3 SV=1)

HSP 1 Score: 1416.7 bits (3666), Expect = 0.0e+00
Identity = 676/705 (95.89%), Postives = 690/705 (97.87%), Query Frame = 0

Query: 94  RTSGEQDKVAMGFTDVTASDRLTHLDSSAARKIPRLNATVLGESLASEDDQLILPSDHFS 153
           RTSGEQ +V MGFTD+TA DRLT +DSSAARKIPRLNATVLGESLASEDDQLILPSD FS
Sbjct: 5   RTSGEQAQVVMGFTDLTAPDRLTQVDSSAARKIPRLNATVLGESLASEDDQLILPSDQFS 64

Query: 154 SRAHVSSHHKYLEMYKRSIEDPAGFWSDIASSEFFWKHTWDQQVYSENLDVRKGQIKIEW 213
           SRAHVSSH KYLEMYKRSIEDPAGFWSDIASSEFFWKH WDQ VYSENLDVRKG+IKIEW
Sbjct: 65  SRAHVSSHQKYLEMYKRSIEDPAGFWSDIASSEFFWKHRWDQLVYSENLDVRKGEIKIEW 124

Query: 214 FKGGITNICYNCLDRNIEAGLGEKVALYWEGNEPCFDGTLTYTQLLHKVCQLANFLKDNG 273
           FKGGITNICYNCLDRNIEAGLG+KVALYWEGNEP FDGTLTYT+LLHKVCQLAN+LKDNG
Sbjct: 125 FKGGITNICYNCLDRNIEAGLGDKVALYWEGNEPGFDGTLTYTELLHKVCQLANYLKDNG 184

Query: 274 VRKGDAVVIYMPMLMELPIAMLACARIGAIHSVVFAGFSSESLAQRIIDCKPKIVITCNA 333
           V+KGDAVVIYMPMLMELPIAMLACARIGAIHSVVFAGFSSESLAQRIIDCKPKIVITCNA
Sbjct: 185 VKKGDAVVIYMPMLMELPIAMLACARIGAIHSVVFAGFSSESLAQRIIDCKPKIVITCNA 244

Query: 334 VKRGSKVIHLKDIVDAALTESAQNEVSVATCLTYENESALKSESTKWKEGRDIWWQDVVP 393
           VKRGSKVIHLKDIVDAALTESAQNEVSVATCLTYENESALK EST WKEGRDIWWQDVVP
Sbjct: 245 VKRGSKVIHLKDIVDAALTESAQNEVSVATCLTYENESALKRESTTWKEGRDIWWQDVVP 304

Query: 394 TYPTTCTVEWVDAEHPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPSDVYW 453
           TYPTTCTVEWVDAE PLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKP DVYW
Sbjct: 305 TYPTTCTVEWVDAEFPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPCDVYW 364

Query: 454 CTADCGWITGHSYVTYGPLLNGASVVVYEGAPNYPDPGRCWDIVDKYRVTIFYTAPTLVR 513
           CTADCGWITGHSYVTYGPLLNGASVVVYEGAPNYPDPGRCWDIVDK++VTIFYTAPTLVR
Sbjct: 365 CTADCGWITGHSYVTYGPLLNGASVVVYEGAPNYPDPGRCWDIVDKHKVTIFYTAPTLVR 424

Query: 514 ALMREGNEHVTRYSRKSLRVLGSVGEPINPSAWRWFFNVVGESRCPISDTWWQTETGGFM 573
           +LMREGNEHVTRYSRKSLRVLGSVGEPINPSAWRWFFNVVGESRCPISDTWWQTETGGFM
Sbjct: 425 SLMREGNEHVTRYSRKSLRVLGSVGEPINPSAWRWFFNVVGESRCPISDTWWQTETGGFM 484

Query: 574 ITPLPGAWPQKPGSATFPFFGVQPVLVDEKGNEIEGECSGYLCLKSSWPAAFRTLYNDQE 633
           ITPLPGAWPQKPGSATFPFFGVQPVLVDEKGNEIEGECSGYLCLKSSWPAAFRTLYNDQE
Sbjct: 485 ITPLPGAWPQKPGSATFPFFGVQPVLVDEKGNEIEGECSGYLCLKSSWPAAFRTLYNDQE 544

Query: 634 RYETTYFKPFPGYYFTGDGCTRDRDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQC 693
           RYE TYFKPFPGYYFTGDGCTRD+DGYYWLTGRVDDVINVSGHRIGTAEVESALVSHP C
Sbjct: 545 RYEMTYFKPFPGYYFTGDGCTRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPHC 604

Query: 694 AEAAVVGIEHEVKGQGIYAFVTLVDGVSYSEELRKSLILTVRKQIGAFAAPDKIHWAPGL 753
           AEAAVVGIEHEVKGQGIYAFVTLVDGVSYSEELRKSLILTVRKQIGAFAAPD+IHWAPGL
Sbjct: 605 AEAAVVGIEHEVKGQGIYAFVTLVDGVSYSEELRKSLILTVRKQIGAFAAPDRIHWAPGL 664

Query: 754 PKTRSGKIMRRILRKIASKQLNELGDTSTLADPGVVDQLIELADC 799
           PKTRSGKIMRRILRKIASKQL+ELGDTSTLADPGVVDQLIELADC
Sbjct: 665 PKTRSGKIMRRILRKIASKQLDELGDTSTLADPGVVDQLIELADC 709

BLAST of Sgr011532 vs. ExPASy TrEMBL
Match: A0A6J1D2B5 (Acetate--CoA ligase OS=Momordica charantia OX=3673 GN=LOC111016935 PE=3 SV=1)

HSP 1 Score: 1404.0 bits (3633), Expect = 0.0e+00
Identity = 669/695 (96.26%), Postives = 682/695 (98.13%), Query Frame = 0

Query: 104 MGFTDVTASDRLTHLDSSAARKIPRLNATVLGESLASEDDQLILPSDHFSSRAHVSSHHK 163
           MGFTD+TA DRLT +DSSAARKIPRLNATVLGESLASEDDQLILPSD FSSRAHVSSH K
Sbjct: 1   MGFTDLTAPDRLTQVDSSAARKIPRLNATVLGESLASEDDQLILPSDQFSSRAHVSSHQK 60

Query: 164 YLEMYKRSIEDPAGFWSDIASSEFFWKHTWDQQVYSENLDVRKGQIKIEWFKGGITNICY 223
           YLEMYKRSIEDPAGFWSDIASSEFFWKH WDQ VYSENLDVRKG+IKIEWFKGGITNICY
Sbjct: 61  YLEMYKRSIEDPAGFWSDIASSEFFWKHRWDQLVYSENLDVRKGEIKIEWFKGGITNICY 120

Query: 224 NCLDRNIEAGLGEKVALYWEGNEPCFDGTLTYTQLLHKVCQLANFLKDNGVRKGDAVVIY 283
           NCLDRNIEAGLG+KVALYWEGNEP FDGTLTYT+LLHKVCQLAN+LKDNGV+KGDAVVIY
Sbjct: 121 NCLDRNIEAGLGDKVALYWEGNEPGFDGTLTYTELLHKVCQLANYLKDNGVKKGDAVVIY 180

Query: 284 MPMLMELPIAMLACARIGAIHSVVFAGFSSESLAQRIIDCKPKIVITCNAVKRGSKVIHL 343
           MPMLMELPIAMLACARIGAIHSVVFAGFSSESLAQRIIDCKPKIVITCNAVKRGSKVIHL
Sbjct: 181 MPMLMELPIAMLACARIGAIHSVVFAGFSSESLAQRIIDCKPKIVITCNAVKRGSKVIHL 240

Query: 344 KDIVDAALTESAQNEVSVATCLTYENESALKSESTKWKEGRDIWWQDVVPTYPTTCTVEW 403
           KDIVDAALTESAQNEVSVATCLTYENESALK EST WKEGRDIWWQDVVPTYPTTCTVEW
Sbjct: 241 KDIVDAALTESAQNEVSVATCLTYENESALKRESTTWKEGRDIWWQDVVPTYPTTCTVEW 300

Query: 404 VDAEHPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPSDVYWCTADCGWITG 463
           VDAE PLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKP DVYWCTADCGWITG
Sbjct: 301 VDAEFPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPCDVYWCTADCGWITG 360

Query: 464 HSYVTYGPLLNGASVVVYEGAPNYPDPGRCWDIVDKYRVTIFYTAPTLVRALMREGNEHV 523
           HSYVTYGPLLNGASVVVYEGAPNYPDPGRCWDIVDK++VTIFYTAPTLVR+LMREGNEHV
Sbjct: 361 HSYVTYGPLLNGASVVVYEGAPNYPDPGRCWDIVDKHKVTIFYTAPTLVRSLMREGNEHV 420

Query: 524 TRYSRKSLRVLGSVGEPINPSAWRWFFNVVGESRCPISDTWWQTETGGFMITPLPGAWPQ 583
           TRYSRKSLRVLGSVGEPINPSAWRWFFNVVGESRCPISDTWWQTETGGFMITPLPGAWPQ
Sbjct: 421 TRYSRKSLRVLGSVGEPINPSAWRWFFNVVGESRCPISDTWWQTETGGFMITPLPGAWPQ 480

Query: 584 KPGSATFPFFGVQPVLVDEKGNEIEGECSGYLCLKSSWPAAFRTLYNDQERYETTYFKPF 643
           KPGSATFPFFGVQPVLVDEKGNEIEGECSGYLCLKSSWPAAFRTLYNDQERYE TYFKPF
Sbjct: 481 KPGSATFPFFGVQPVLVDEKGNEIEGECSGYLCLKSSWPAAFRTLYNDQERYEMTYFKPF 540

Query: 644 PGYYFTGDGCTRDRDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGIEH 703
           PGYYFTGDGCTRD+DGYYWLTGRVDDVINVSGHRIGTAEVESALVSHP CAEAAVVGIEH
Sbjct: 541 PGYYFTGDGCTRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPHCAEAAVVGIEH 600

Query: 704 EVKGQGIYAFVTLVDGVSYSEELRKSLILTVRKQIGAFAAPDKIHWAPGLPKTRSGKIMR 763
           EVKGQGIYAFVTLVDGVSYSEELRKSLILTVRKQIGAFAAPD+IHWAPGLPKTRSGKIMR
Sbjct: 601 EVKGQGIYAFVTLVDGVSYSEELRKSLILTVRKQIGAFAAPDRIHWAPGLPKTRSGKIMR 660

Query: 764 RILRKIASKQLNELGDTSTLADPGVVDQLIELADC 799
           RILRKIASKQL+ELGDTSTLADPGVVDQLIELADC
Sbjct: 661 RILRKIASKQLDELGDTSTLADPGVVDQLIELADC 695

BLAST of Sgr011532 vs. ExPASy TrEMBL
Match: A0A0A0L7C4 (Acetate--CoA ligase OS=Cucumis sativus OX=3659 GN=Csa_3G253500 PE=3 SV=1)

HSP 1 Score: 1395.2 bits (3610), Expect = 0.0e+00
Identity = 696/817 (85.19%), Postives = 728/817 (89.11%), Query Frame = 0

Query: 1   MLNTVATSSYG--RPFPASFNPTLIIFPSAAIPLPNN-SNPKIHNRKQLNYNQRHGHVNT 60
           MLN + TSS    RPFPAS NP  I+FPSAA  + ++  NP  HN K+        H+NT
Sbjct: 1   MLNLIVTSSCNAVRPFPASSNPG-ILFPSAAAAINSDYPNPYFHNCKE-------RHINT 60

Query: 61  FHNYYYLN--------SCLPPSCCARSFICSSW--TSASGFCCSLSRT-----SGEQDK- 120
           FHN+ Y N        S    SC  R     S+  +S S F  SL+R       G +DK 
Sbjct: 61  FHNHLYFNSTSSSFSSSTTTSSCLLRPPFAGSFLSSSLSTFSSSLTRIRGSDGDGRRDKN 120

Query: 121 VAM-GFTDVTASDRLTHLDSSAARKIPRLNATVLGESLASEDDQLILPSDHFSSRAHVSS 180
           ++M G TDV A      L+SSAARKIPRLNA VLGES A+EDDQLI PSD FSS AH+SS
Sbjct: 121 LSMGGLTDVAA------LESSAARKIPRLNAAVLGESPATEDDQLIFPSDLFSSNAHISS 180

Query: 181 HHKYLEMYKRSIEDPAGFWSDIASSEFFWKHTWDQQVYSENLDVRKGQIKIEWFKGGITN 240
           +H+YLEMYKRSIEDPAGFWSDIASSEFFWK  WDQQVYSENLDVRKG+IKIEWFKGGITN
Sbjct: 181 YHQYLEMYKRSIEDPAGFWSDIASSEFFWKQKWDQQVYSENLDVRKGEIKIEWFKGGITN 240

Query: 241 ICYNCLDRNIEAGLGEKVALYWEGNEPCFDGTLTYTQLLHKVCQLANFLKDNGVRKGDAV 300
           ICYNCLDRNIEAGLGEK+ALYWEGNEP FDGTLTY +LLHKVCQLANFLKD GVRKGDAV
Sbjct: 241 ICYNCLDRNIEAGLGEKIALYWEGNEPGFDGTLTYIELLHKVCQLANFLKDKGVRKGDAV 300

Query: 301 VIYMPMLMELPIAMLACARIGAIHSVVFAGFSSESLAQRIIDCKPKIVITCNAVKRGSKV 360
           VIYMPMLMELPIAMLACARIGA+HSVVFAGFSSESLAQRIIDCKPKIVITCNAVKRGSK 
Sbjct: 301 VIYMPMLMELPIAMLACARIGAVHSVVFAGFSSESLAQRIIDCKPKIVITCNAVKRGSKA 360

Query: 361 IHLKDIVDAALTESAQNEVSVATCLTYENESALKSESTKWKEGRDIWWQDVVPTYPTTCT 420
           IHLKDIVDAAL ESAQN VSVATCL+YENESAL  ESTKWKEGRDIWWQDVVPTYPTTC 
Sbjct: 361 IHLKDIVDAALIESAQNGVSVATCLSYENESALNRESTKWKEGRDIWWQDVVPTYPTTCP 420

Query: 421 VEWVDAEHPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPSDVYWCTADCGW 480
           VEWVDAE PLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPSDVYWCTADCGW
Sbjct: 421 VEWVDAEFPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPSDVYWCTADCGW 480

Query: 481 ITGHSYVTYGPLLNGASVVVYEGAPNYPDPGRCWDIVDKYRVTIFYTAPTLVRALMREGN 540
           ITGHSYVTYGPLLNGASVVV+EGAPNYPD GRCWDIVDKY+VTIFYTAPTLVR+LMREGN
Sbjct: 481 ITGHSYVTYGPLLNGASVVVFEGAPNYPDHGRCWDIVDKYKVTIFYTAPTLVRSLMREGN 540

Query: 541 EHVTRYSRKSLRVLGSVGEPINPSAWRWFFNVVGESRCPISDTWWQTETGGFMITPLPGA 600
           EHVTRYSRKSLRVLGSVGEPINPSAWRWFFNVVGESRCPISDTWWQTETGGFMITPLPGA
Sbjct: 541 EHVTRYSRKSLRVLGSVGEPINPSAWRWFFNVVGESRCPISDTWWQTETGGFMITPLPGA 600

Query: 601 WPQKPGSATFPFFGVQPVLVDEKGNEIEGECSGYLCLKSSWPAAFRTLYNDQERYETTYF 660
           WPQKPGSATFPFFGVQPVLVDEKGNEIEGECSGYLCLKSSWP+AFRTLYNDQERYE TYF
Sbjct: 601 WPQKPGSATFPFFGVQPVLVDEKGNEIEGECSGYLCLKSSWPSAFRTLYNDQERYEVTYF 660

Query: 661 KPFPGYYFTGDGCTRDRDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVG 720
           KPFPGYYFTGDGCTRD+DGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVG
Sbjct: 661 KPFPGYYFTGDGCTRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVG 720

Query: 721 IEHEVKGQGIYAFVTLVDGVSYSEELRKSLILTVRKQIGAFAAPDKIHWAPGLPKTRSGK 780
           +EHEVKGQGIYAFVTLVDGVSYSEELRKSLILTVRKQIGAFAAPDKIHWAPGLPKTRSGK
Sbjct: 721 VEHEVKGQGIYAFVTLVDGVSYSEELRKSLILTVRKQIGAFAAPDKIHWAPGLPKTRSGK 780

Query: 781 IMRRILRKIASKQLNELGDTSTLADPGVVDQLIELAD 798
           IMRRILRKIASKQL+ELGDTSTLADPGVV+QLIELAD
Sbjct: 781 IMRRILRKIASKQLDELGDTSTLADPGVVNQLIELAD 803

BLAST of Sgr011532 vs. TAIR 10
Match: AT5G36880.1 (acetyl-CoA synthetase )

HSP 1 Score: 1193.7 bits (3087), Expect = 0.0e+00
Identity = 556/692 (80.35%), Postives = 626/692 (90.46%), Query Frame = 0

Query: 111 ASDRLTHLDS-----SAARKIPRLNATVLGESLASEDDQLILPSDHFSSRAHVSSHHKYL 170
           +S+ L H++S     S A KI +LNA VLGESLASE++ L+ PS  FS +A VSS  +Y+
Sbjct: 2   SSNSLRHVESMSQLPSGAGKISQLNAVVLGESLASEENDLVFPSKEFSGQALVSSPQQYM 61

Query: 171 EMYKRSIEDPAGFWSDIASSEFFWKHTWDQQVYSENLDVRKGQIKIEWFKGGITNICYNC 230
           EM+KRS++DPA FWSDIA SEF+WK  W  QV+SENLDVRKG I IEWFKGGITNICYNC
Sbjct: 62  EMHKRSMDDPAAFWSDIA-SEFYWKQKWGDQVFSENLDVRKGPISIEWFKGGITNICYNC 121

Query: 231 LDRNIEAGLGEKVALYWEGNEPCFDGTLTYTQLLHKVCQLANFLKDNGVRKGDAVVIYMP 290
           LD+N+EAGLG+K A++WEGNE   D +LTY++LL +VCQLAN+LKDNGV+KGDAVVIY+P
Sbjct: 122 LDKNVEAGLGDKTAIHWEGNELGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLP 181

Query: 291 MLMELPIAMLACARIGAIHSVVFAGFSSESLAQRIIDCKPKIVITCNAVKRGSKVIHLKD 350
           MLMELPIAMLACARIGA+HSVVFAGFS++SLAQRI+DCKP +++TCNAVKRG K I+LK 
Sbjct: 182 MLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILTCNAVKRGPKTINLKA 241

Query: 351 IVDAALTESAQNEVSVATCLTYENESALKSESTKWKEGRDIWWQDVVPTYPTTCTVEWVD 410
           IVDAAL +S+++ VSV  CLTY+N  A   E+TKW+ GRD+WWQDV+  YPT+C VEWVD
Sbjct: 242 IVDAALDQSSKDGVSVGICLTYDNSLATTRENTKWQNGRDVWWQDVISQYPTSCEVEWVD 301

Query: 411 AEHPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPSDVYWCTADCGWITGHS 470
           AE PLFLLYTSGSTGKPKGVLHTTGGYM+YTATTFKYAFDYK +DVYWCTADCGWITGHS
Sbjct: 302 AEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKYAFDYKSTDVYWCTADCGWITGHS 361

Query: 471 YVTYGPLLNGASVVVYEGAPNYPDPGRCWDIVDKYRVTIFYTAPTLVRALMREGNEHVTR 530
           YVTYGP+LNGA+VVV+EGAPNYPDPGRCWDIVDKY+V+IFYTAPTLVR+LMR+ ++ VTR
Sbjct: 362 YVTYGPMLNGATVVVFEGAPNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTR 421

Query: 531 YSRKSLRVLGSVGEPINPSAWRWFFNVVGESRCPISDTWWQTETGGFMITPLPGAWPQKP 590
           +SRKSLRVLGSVGEPINPSAWRWFFNVVG+SRCPISDTWWQTETGGFMITPLPGAWPQKP
Sbjct: 422 HSRKSLRVLGSVGEPINPSAWRWFFNVVGDSRCPISDTWWQTETGGFMITPLPGAWPQKP 481

Query: 591 GSATFPFFGVQPVLVDEKGNEIEGECSGYLCLKSSWPAAFRTLYNDQERYETTYFKPFPG 650
           GSATFPFFGVQPV+VDEKGNEIEGECSGYLC+K SWP AFRTL+ D ERYETTYFKPF G
Sbjct: 482 GSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGSWPGAFRTLFGDHERYETTYFKPFAG 541

Query: 651 YYFTGDGCTRDRDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGIEHEV 710
           YYF+GDGC+RD+DGYYWLTGRVDDVINVSGHRIGTAEVESALV HPQCAEAAVVGIEHEV
Sbjct: 542 YYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEV 601

Query: 711 KGQGIYAFVTLVDGVSYSEELRKSLILTVRKQIGAFAAPDKIHWAPGLPKTRSGKIMRRI 770
           KGQGIYAFVTL++GV YSEELRKSL+L VR QIGAFAAPD+IHWAPGLPKTRSGKIMRRI
Sbjct: 602 KGQGIYAFVTLLEGVPYSEELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRI 661

Query: 771 LRKIASKQLNELGDTSTLADPGVVDQLIELAD 798
           LRKIAS+QL ELGDTSTLADP VVDQLI LAD
Sbjct: 662 LRKIASRQLEELGDTSTLADPSVVDQLIALAD 692

BLAST of Sgr011532 vs. TAIR 10
Match: AT5G36880.2 (acetyl-CoA synthetase )

HSP 1 Score: 1193.7 bits (3087), Expect = 0.0e+00
Identity = 556/692 (80.35%), Postives = 626/692 (90.46%), Query Frame = 0

Query: 111 ASDRLTHLDS-----SAARKIPRLNATVLGESLASEDDQLILPSDHFSSRAHVSSHHKYL 170
           +S+ L H++S     S A KI +LNA VLGESLASE++ L+ PS  FS +A VSS  +Y+
Sbjct: 52  SSNSLRHVESMSQLPSGAGKISQLNAVVLGESLASEENDLVFPSKEFSGQALVSSPQQYM 111

Query: 171 EMYKRSIEDPAGFWSDIASSEFFWKHTWDQQVYSENLDVRKGQIKIEWFKGGITNICYNC 230
           EM+KRS++DPA FWSDIA SEF+WK  W  QV+SENLDVRKG I IEWFKGGITNICYNC
Sbjct: 112 EMHKRSMDDPAAFWSDIA-SEFYWKQKWGDQVFSENLDVRKGPISIEWFKGGITNICYNC 171

Query: 231 LDRNIEAGLGEKVALYWEGNEPCFDGTLTYTQLLHKVCQLANFLKDNGVRKGDAVVIYMP 290
           LD+N+EAGLG+K A++WEGNE   D +LTY++LL +VCQLAN+LKDNGV+KGDAVVIY+P
Sbjct: 172 LDKNVEAGLGDKTAIHWEGNELGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLP 231

Query: 291 MLMELPIAMLACARIGAIHSVVFAGFSSESLAQRIIDCKPKIVITCNAVKRGSKVIHLKD 350
           MLMELPIAMLACARIGA+HSVVFAGFS++SLAQRI+DCKP +++TCNAVKRG K I+LK 
Sbjct: 232 MLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILTCNAVKRGPKTINLKA 291

Query: 351 IVDAALTESAQNEVSVATCLTYENESALKSESTKWKEGRDIWWQDVVPTYPTTCTVEWVD 410
           IVDAAL +S+++ VSV  CLTY+N  A   E+TKW+ GRD+WWQDV+  YPT+C VEWVD
Sbjct: 292 IVDAALDQSSKDGVSVGICLTYDNSLATTRENTKWQNGRDVWWQDVISQYPTSCEVEWVD 351

Query: 411 AEHPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPSDVYWCTADCGWITGHS 470
           AE PLFLLYTSGSTGKPKGVLHTTGGYM+YTATTFKYAFDYK +DVYWCTADCGWITGHS
Sbjct: 352 AEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKYAFDYKSTDVYWCTADCGWITGHS 411

Query: 471 YVTYGPLLNGASVVVYEGAPNYPDPGRCWDIVDKYRVTIFYTAPTLVRALMREGNEHVTR 530
           YVTYGP+LNGA+VVV+EGAPNYPDPGRCWDIVDKY+V+IFYTAPTLVR+LMR+ ++ VTR
Sbjct: 412 YVTYGPMLNGATVVVFEGAPNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTR 471

Query: 531 YSRKSLRVLGSVGEPINPSAWRWFFNVVGESRCPISDTWWQTETGGFMITPLPGAWPQKP 590
           +SRKSLRVLGSVGEPINPSAWRWFFNVVG+SRCPISDTWWQTETGGFMITPLPGAWPQKP
Sbjct: 472 HSRKSLRVLGSVGEPINPSAWRWFFNVVGDSRCPISDTWWQTETGGFMITPLPGAWPQKP 531

Query: 591 GSATFPFFGVQPVLVDEKGNEIEGECSGYLCLKSSWPAAFRTLYNDQERYETTYFKPFPG 650
           GSATFPFFGVQPV+VDEKGNEIEGECSGYLC+K SWP AFRTL+ D ERYETTYFKPF G
Sbjct: 532 GSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGSWPGAFRTLFGDHERYETTYFKPFAG 591

Query: 651 YYFTGDGCTRDRDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGIEHEV 710
           YYF+GDGC+RD+DGYYWLTGRVDDVINVSGHRIGTAEVESALV HPQCAEAAVVGIEHEV
Sbjct: 592 YYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHEV 651

Query: 711 KGQGIYAFVTLVDGVSYSEELRKSLILTVRKQIGAFAAPDKIHWAPGLPKTRSGKIMRRI 770
           KGQGIYAFVTL++GV YSEELRKSL+L VR QIGAFAAPD+IHWAPGLPKTRSGKIMRRI
Sbjct: 652 KGQGIYAFVTLLEGVPYSEELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRI 711

Query: 771 LRKIASKQLNELGDTSTLADPGVVDQLIELAD 798
           LRKIAS+QL ELGDTSTLADP VVDQLI LAD
Sbjct: 712 LRKIASRQLEELGDTSTLADPSVVDQLIALAD 742

BLAST of Sgr011532 vs. TAIR 10
Match: AT5G23050.1 (acyl-activating enzyme 17 )

HSP 1 Score: 160.6 bits (405), Expect = 5.2e-39
Identity = 156/594 (26.26%), Postives = 254/594 (42.76%), Query Frame = 0

Query: 212 EWFKGGITNICYNCLDRNIEAGLGEKVALYW--EGNEPCFDGTLTYTQLLHKVCQLANFL 271
           +W  G   N   NCL    +  L + + + W  EG++     T+T  +L  +V   A+ L
Sbjct: 161 KWLPGAYLNPARNCLTNGFKRRL-DDIVIRWRDEGSDDLPVNTMTLLELRSQVWLAAHAL 220

Query: 272 KDNGVRKGDAVVIYMPMLMELPIAMLACARIGAIHSVVFAGFSSESLAQRIIDCKPKIVI 331
              G+ +  A+ + MPM +E  I  LA    G +   +   FS   ++ R+   K K + 
Sbjct: 221 SALGLEEESAIAVDMPMNVESVIIYLAIVLAGHVVVSIADSFSPREISTRLKISKAKAIF 280

Query: 332 TCNAVKRGSKVIHL-KDIVDAALTESAQNEVSVATCLTYENESALKSESTKWKEGRDIWW 391
           T + + RG K I L + +VDA        E  +A  +     S       K +E +D+ W
Sbjct: 281 TQDVIIRGDKSIPLYRRVVDA--------EAPLAIVVPARGSSC----RMKLRE-KDLSW 340

Query: 392 QDVVPTYPTTCTVEWVDAEHP----LFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAF 451
            + +        VE+V AE P      +L++SG+TG+PK +  T    +   A  + +  
Sbjct: 341 NNFLGNARNLRGVEYVAAEKPAGAYTNILFSSGTTGEPKAIPWTNISPLKSAADAWCH-L 400

Query: 452 DYKPSDVYWCTADCGWITGHSYVTYGPLLNGASVVVYEGAPNYPDPGRCWDIVDKYRVTI 511
           D +  DV     + GW+ G  ++ Y  L+NGA + +Y G+P  P   +    V    V++
Sbjct: 401 DVQRGDVVAWPTNLGWMMG-PWLVYASLINGACMGLYNGSPLGPTFAK---FVQDAEVSV 460

Query: 512 FYTAPTLVRALMREGNEHVTRYSRKSLRVLGSVGEPINPSAWRWFFNVVGESRC-PISDT 571
               P++VR    + +   + Y    +R  GS GE  N   + W   ++G +   P+ + 
Sbjct: 461 LGVIPSIVRTW--QNSNSTSGYDWSRIRCFGSTGEASNIDEYLW---LMGRAHYKPVIEY 520

Query: 572 WWQTETGGFMITPLPGAWPQKPGSATF--PFFGVQPVLVDEKGNEIEGECSGY--LCLKS 631
              TE GG  I+   G+  Q    A F     G +  ++DE  N I    +G   L L  
Sbjct: 521 CGGTEIGGSFIS---GSLLQPQSLAAFSTAAMGCKLFILDEDSNPIPPYAAGVGELALCP 580

Query: 632 SWPAAFRTLYNDQERYETTYFKPFPGYYFT-----GDGCTRDRDGYYWLTGRVDDVINVS 691
               A  TL N    +   YF+  P +        GD   R   GYY   GR DD +N+ 
Sbjct: 581 HMFGASSTLLNG--NHFKVYFQGMPTFQGQILRRHGDLFERTSKGYYRAHGRADDTMNLG 640

Query: 692 GHRIGTAEVESALVS-HPQCAEAAVVGIEHEVKGQGIYAFVTLVDGVSYSEE----LRKS 751
           G ++G+ E+E    S      E A +G+     G        +     +S      L+KS
Sbjct: 641 GIKVGSIEIERVCNSVDDSVLETAAIGVPPPSGGPEQLVIAVVFKSPEFSNPDLNLLKKS 700

Query: 752 LILTVRKQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRKIASKQLNELGDTSTL 784
               ++K++        +   P LP+T + K+MRR+LR    +QL + G  S L
Sbjct: 701 FNSEIQKKLNPLFKVSSVVTLPSLPRTATNKVMRRVLR----QQLTQTGLNSKL 721

BLAST of Sgr011532 vs. TAIR 10
Match: AT5G38120.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 137.1 bits (344), Expect = 6.1e-32
Identity = 129/537 (24.02%), Postives = 234/537 (43.58%), Query Frame = 0

Query: 250 DGTLTYTQLLHKVCQLAN-FLKDNGVRKGDAVVIYMPMLMELPIAMLACARIGAIHSVVF 309
           D  ++++ L   V ++A+  L D G+R+GD V++  P  + +PI  L+   +GA+ +   
Sbjct: 62  DHRISFSDLWMAVDRVADCLLHDVGIRRGDVVLVLSPNTISIPIVCLSVMSLGAVLTTAN 121

Query: 310 AGFSSESLAQRIIDCKPKIVITCN--AVKRGSKVIHLKDIVDAALTESAQNEVSVATCLT 369
              ++  + ++I D  PK+  T    A K  S  I +       + E  ++ + V   L 
Sbjct: 122 PLNTASEILRQIADSNPKLAFTTPELAPKIASSGISI-------VLERVEDTLRVPRGLK 181

Query: 370 YENESALKSESTKWKEGRDIWWQDVVPTYPTTCTVEWVDAEHPLFLLYTSGSTGKPKGVL 429
                 L     K   G+ +  Q              V  +    LLY+SG+TG+ KGV 
Sbjct: 182 VVGN--LTEMMKKEPSGQAVRNQ--------------VHKDDTAMLLYSSGTTGRSKGVN 241

Query: 430 HTTGGYMVYTATTFKYAFDYKPSDVYWCTADCGWITGHSYVTYGPLLNGASVVVYEGAPN 489
            + G  + + A      F+ +P   + CT       G        L  G +VV+    P 
Sbjct: 242 SSHGNLIAHVARYIAEPFE-QPQQTFICTVPLFHTFGLLNFVLATLALGTTVVI---LPR 301

Query: 490 YPDPGRCWDIVDKYRVTIFYTAPTLVRALMREGNEHVTRYSRKSLRVLGSVGEPINPSAW 549
           + D G     V+KYR T     P ++  ++ + ++ + +Y    LR +   G P++    
Sbjct: 302 F-DLGEMMAAVEKYRATTLILVPPVLVTMINKADQIMKKYDVSFLRTVRCGGAPLSKEVT 361

Query: 550 RWFFNVVGESRCPISDTWWQTETGGFMITPLPGAW--------PQKPGSATFPFFGVQPV 609
           + F       + P  D +      G+ +T   GA          ++ G+      GV+  
Sbjct: 362 QGFM-----KKYPTVDVY-----QGYALTESNGAGASIESVEESRRYGAVGLLSCGVEAR 421

Query: 610 LVDEKGNEIEG-ECSGYLCLKSSWPAAFRTLYNDQERYETTYFKPFPGYYFTGDGCTRDR 669
           +VD    ++ G   +G L LK   P+  +  + ++E   T+      G+  TGD C  D 
Sbjct: 422 IVDPNTGQVMGLNQTGELWLKG--PSIAKGYFRNEEEIITS-----EGWLKTGDLCYIDN 481

Query: 670 DGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGIEHEVKGQGIYAFVTLV 729
           DG+ ++  R+ ++I   G+++  AE+E+ L++HP   +AAV+    +  GQ   A+V   
Sbjct: 482 DGFLFIVDRLKELIKYKGYQVPPAELEALLLNHPDILDAAVIPFPDKEAGQFPMAYVARK 541

Query: 730 DGVSYSEELRKSLILTVRKQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRKIASKQL 775
              +  E   K +I  + KQ+  +    K+ +   +PKT SGK +R+ L K A  ++
Sbjct: 542 PESNLCE---KKVIDFISKQVAPYKKIRKVAFIDSIPKTPSGKTLRKDLIKFAISKI 550

BLAST of Sgr011532 vs. TAIR 10
Match: AT1G20500.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 136.7 bits (343), Expect = 8.0e-32
Identity = 127/525 (24.19%), Postives = 229/525 (43.62%), Query Frame = 0

Query: 253 LTYTQLLHKVCQLANFL-KDNGVRKGDAVVIYMPMLMELPIAMLACARIGAIHSVVFAGF 312
           LT++ L   V ++A+ L  + G+R+GD V+I  P  + +P+  L+   +GA+ +      
Sbjct: 64  LTFSDLWRAVDRVADCLYHEVGIRRGDVVLILSPNSIFIPVVCLSVMSLGAVFTTANTLN 123

Query: 313 SSESLAQRIIDCKPKIVITCNAVKRGSKVIHLKDIVDAALTESAQNEVSVATCLTYENES 372
           +S  ++++I D  P +V T   +     V     + D  + +   + + V   L   +E 
Sbjct: 124 TSGEISKQIADSNPTLVFTTRQLAPKLPVAISVVLTDDEVYQELTSAIRVVGIL---SEM 183

Query: 373 ALKSESTKWKEGRDIWWQDVVPTYPTTCTVEWVDAEHPLFLLYTSGSTGKPKGVLHTTGG 432
             K  S +    R                   V+ +    +LY+SG+TG  KGV+     
Sbjct: 184 VKKEPSGQRVRDR-------------------VNQDDTAMMLYSSGTTGPSKGVI---SS 243

Query: 433 YMVYTATTFKYAFD-YKPSDVYWCTADCGWITGHSYVTYGPLLNGASVVVYEGAPNYPDP 492
           +   TA   ++  D  K  D++ CT       G      G +  G++VV+      +   
Sbjct: 244 HRNLTAHVARFISDNLKRDDIFICTVPMFHTYGLLTFAMGTVALGSTVVILRRFQLHD-- 303

Query: 493 GRCWDIVDKYRVTIFYTAPTLVRALMREGNEHVTRYSRKSLRVLGSVGEPINPSAWRWFF 552
               D V+K+R T    AP ++ A++ + +    +Y   SL+ +   G P++      F 
Sbjct: 304 --MMDAVEKHRATALALAPPVLVAMINDADLIKAKYDLSSLKTVRCGGAPLSKEVTEGFL 363

Query: 553 NVVGESRCPISDTWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVLVDEKGNEIEG- 612
                    I   +  TE+ G           ++ G+A      V+  +VD       G 
Sbjct: 364 EKY--PTVDILQGYALTESNGGGAFTNSAEESRRYGTAGTLTSDVEARIVDPNTGRFMGI 423

Query: 613 ECSGYLCLKSSWPAAFRTLYNDQERYETTYFKPFPGYYFTGDGCTRDRDGYYWLTGRVDD 672
             +G L LK   P+  +  + +QE    T      G+  TGD C  D DG+ ++  R+ +
Sbjct: 424 NQTGELWLKG--PSISKGYFKNQEATNETI--NLEGWLKTGDLCYIDEDGFLFVVDRLKE 483

Query: 673 VINVSGHRIGTAEVESALVSHPQCAEAAVVGIEHEVKGQGIYAFVTLVDGVSYSEELRKS 732
           +I   G+++  AE+E+ L++HP   +AAV+    +  GQ   A+V      + SE   K 
Sbjct: 484 LIKYKGYQVPPAELEALLITHPDILDAAVIPFPDKEAGQYPMAYVVRKHESNLSE---KQ 543

Query: 733 LILTVRKQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRKIASKQL 775
           +I  + KQ+  +    K+ +   +PKT SGK +R+ L K+A+ +L
Sbjct: 544 VIDFISKQVAPYKKIRKVSFINSIPKTASGKTLRKDLIKLATSKL 550