Sgr011474 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr011474 vs. NCBI nr
Match: KAG6593896.1 (Vacuole membrane protein KMS1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 704.9 bits (1818), Expect = 3.9e-199
Identity = 362/424 (85.38%), Postives = 384/424 (90.57%), Query Frame = 0

Query: 1   MGSGEEAASIASYASEVSISG----------------------KYSSL-----AKRSIAY 60
           MG+GEEAAS+A++ SEVSIS                       K+ +L      K SIAY
Sbjct: 1   MGTGEEAASLAAHDSEVSISELCRKHKQDLDNLTLVNQPFKTLKFFTLGVLQYGKHSIAY 60

Query: 61  ILAKGGWLLLLNVIIGAAGILILTIEGPHEKHVEEVVKYFDYGLWWVILGVASSIGLGSG 120
           ILAKGGWLLLLN+IIGAAGIL+LTIEGPHEKHVEE+VKYFDYGLWWVILGVASSIGLGSG
Sbjct: 61  ILAKGGWLLLLNLIIGAAGILVLTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG 120

Query: 121 LHTFVLYLGPHIGFFTIKAVQCGRVDLKSAPYDTIQLKRGPSWLEKDCSEFGPPIFTSQV 180
           LHTFVLYLGPHIGFFTIKAVQCGRVDLKSA YDTIQLKRGPSWLEKDCSEFGPP+FTSQV
Sbjct: 121 LHTFVLYLGPHIGFFTIKAVQCGRVDLKSAVYDTIQLKRGPSWLEKDCSEFGPPVFTSQV 180

Query: 181 PLSSILPMVQIEAILWGIGTALGELPPYFISRAARLSGGRSEAMEELDASSRETSGLIST 240
           PLSSILPMVQIEAILWGIGT+LGELPPYFISRAARLSGGRSEAMEELDASSRET GLIST
Sbjct: 181 PLSSILPMVQIEAILWGIGTSLGELPPYFISRAARLSGGRSEAMEELDASSRETDGLIST 240

Query: 241 YLNRVKRWFLSHAQHLNFLSILLLASVPNPLFDLAGIMCGQFGIPFWEFFLATLVGKAII 300
           YLNR+KRWFLSHAQHLNFL+ILLLAS+PNPLFDLAGIMCGQFGIPFWEFF ATLVGKAII
Sbjct: 241 YLNRIKRWFLSHAQHLNFLTILLLASIPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII 300

Query: 301 KTHIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSSILPELIAKLNSVKAKYLKAPSHA 360
           KTHIQTIFIIAVCNNQLLDWIENEL+WILSFVPGFSSILP L+AKLN+VK+KYLKAPS  
Sbjct: 301 KTHIQTIFIIAVCNNQLLDWIENELLWILSFVPGFSSILPGLVAKLNAVKSKYLKAPSQV 360

Query: 361 AANAKAKKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDKELAALTNKKVPAS 398
           AANAKAKKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQD+ELAA+T+ KV AS
Sbjct: 361 AANAKAKKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDEELAAMTD-KVSAS 420

BLAST of Sgr011474 vs. NCBI nr
Match: XP_023514621.1 (vacuole membrane protein KMS1-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 704.5 bits (1817), Expect = 5.1e-199
Identity = 361/424 (85.14%), Postives = 384/424 (90.57%), Query Frame = 0

Query: 1   MGSGEEAASIASYASEVSISG----------------------KYSSL-----AKRSIAY 60
           MG+GEEAAS+A++ SEVSIS                       K+ +L      K SIAY
Sbjct: 1   MGTGEEAASLAAHDSEVSISELCRKHKQDLDNLTLVNQPFKTLKFFTLGVLQYGKHSIAY 60

Query: 61  ILAKGGWLLLLNVIIGAAGILILTIEGPHEKHVEEVVKYFDYGLWWVILGVASSIGLGSG 120
           ILAKGGWLLLLN+IIGAAGIL+LTIEGPHEKHVEE+VKYFDYGLWWVILGVASSIGLGSG
Sbjct: 61  ILAKGGWLLLLNLIIGAAGILVLTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG 120

Query: 121 LHTFVLYLGPHIGFFTIKAVQCGRVDLKSAPYDTIQLKRGPSWLEKDCSEFGPPIFTSQV 180
           LHTFVLYLGPHIGFFTIKAVQCGRVDLKSA YDTIQLKRGPSWLEKDCSEFGPP+FTSQV
Sbjct: 121 LHTFVLYLGPHIGFFTIKAVQCGRVDLKSAVYDTIQLKRGPSWLEKDCSEFGPPVFTSQV 180

Query: 181 PLSSILPMVQIEAILWGIGTALGELPPYFISRAARLSGGRSEAMEELDASSRETSGLIST 240
           PLSSILPMVQIEAILWGIGT+LGELPPYFISRAARLSGGRSEAMEELDASSRET GLIST
Sbjct: 181 PLSSILPMVQIEAILWGIGTSLGELPPYFISRAARLSGGRSEAMEELDASSRETDGLIST 240

Query: 241 YLNRVKRWFLSHAQHLNFLSILLLASVPNPLFDLAGIMCGQFGIPFWEFFLATLVGKAII 300
           YLNR+KRWFLSHAQHLNFL+ILLLAS+PNPLFDLAGIMCGQFG+PFWEFF ATLVGKAII
Sbjct: 241 YLNRIKRWFLSHAQHLNFLTILLLASIPNPLFDLAGIMCGQFGVPFWEFFFATLVGKAII 300

Query: 301 KTHIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSSILPELIAKLNSVKAKYLKAPSHA 360
           KTHIQTIFIIAVCNNQLLDWIENEL+WILSFVPGFSSILP L+AKLN+VK+KYLKAPS  
Sbjct: 301 KTHIQTIFIIAVCNNQLLDWIENELLWILSFVPGFSSILPGLVAKLNAVKSKYLKAPSQV 360

Query: 361 AANAKAKKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDKELAALTNKKVPAS 398
           AANAKAKKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQD+ELAA+T+ KV AS
Sbjct: 361 AANAKAKKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDEELAAMTD-KVSAS 420

BLAST of Sgr011474 vs. NCBI nr
Match: KAG7026241.1 (Vacuole membrane protein KMS1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 703.0 bits (1813), Expect = 1.5e-198
Identity = 361/424 (85.14%), Postives = 383/424 (90.33%), Query Frame = 0

Query: 1   MGSGEEAASIASYASEVSISG----------------------KYSSL-----AKRSIAY 60
           MG+GEEAAS+A++ SEVSIS                       K+ +L      K SIAY
Sbjct: 1   MGTGEEAASLAAHDSEVSISELCRKHKQDLDNLTLVNQPFKTLKFFTLGVLQYGKHSIAY 60

Query: 61  ILAKGGWLLLLNVIIGAAGILILTIEGPHEKHVEEVVKYFDYGLWWVILGVASSIGLGSG 120
           ILAKGGWLLLLN+IIGAAGIL+LTIEGPHEKHVEE+VKYFDYGLWWVILGVASSIGLGSG
Sbjct: 61  ILAKGGWLLLLNLIIGAAGILVLTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG 120

Query: 121 LHTFVLYLGPHIGFFTIKAVQCGRVDLKSAPYDTIQLKRGPSWLEKDCSEFGPPIFTSQV 180
           LHTFVLYLGPHIGFFTIKAVQCGRVDLKSA YDTIQLKRGPSWLEKDCSEFGPP+FTSQV
Sbjct: 121 LHTFVLYLGPHIGFFTIKAVQCGRVDLKSAVYDTIQLKRGPSWLEKDCSEFGPPVFTSQV 180

Query: 181 PLSSILPMVQIEAILWGIGTALGELPPYFISRAARLSGGRSEAMEELDASSRETSGLIST 240
           PLSSILPMVQIEAILWGIGT+LGELPPYFISRAARLSGGRSEAMEELDASSRET GLIST
Sbjct: 181 PLSSILPMVQIEAILWGIGTSLGELPPYFISRAARLSGGRSEAMEELDASSRETDGLIST 240

Query: 241 YLNRVKRWFLSHAQHLNFLSILLLASVPNPLFDLAGIMCGQFGIPFWEFFLATLVGKAII 300
           YLNR+KRWFLSHAQHLNFL+ILLLAS+PNPLFDLAGIMCGQFGIPFWEFF ATLVGKAII
Sbjct: 241 YLNRIKRWFLSHAQHLNFLTILLLASIPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII 300

Query: 301 KTHIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSSILPELIAKLNSVKAKYLKAPSHA 360
           KTHIQTIFIIAVCNNQLLDWIENEL+WILSFVPGFSSILP L+AKLN+VK+KYLKAPS  
Sbjct: 301 KTHIQTIFIIAVCNNQLLDWIENELLWILSFVPGFSSILPGLVAKLNAVKSKYLKAPSQV 360

Query: 361 AANAKAKKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDKELAALTNKKVPAS 398
           AANAKA KWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQD+ELAA+T+ KV AS
Sbjct: 361 AANAKANKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDEELAAMTD-KVSAS 420

BLAST of Sgr011474 vs. NCBI nr
Match: XP_022930479.1 (vacuole membrane protein KMS1-like [Cucurbita moschata])

HSP 1 Score: 702.6 bits (1812), Expect = 1.9e-198
Identity = 361/424 (85.14%), Postives = 383/424 (90.33%), Query Frame = 0

Query: 1   MGSGEEAASIASYASEVSISG----------------------KYSSL-----AKRSIAY 60
           MG+GEEAAS+A++ SEVSIS                       K+ +L      K SIAY
Sbjct: 1   MGTGEEAASLAAHDSEVSISELCRKHKQDLDNLTLVNQPFKTLKFFTLGVLQYGKHSIAY 60

Query: 61  ILAKGGWLLLLNVIIGAAGILILTIEGPHEKHVEEVVKYFDYGLWWVILGVASSIGLGSG 120
           ILAKGGWLLLLN+IIGAAGIL+LTIEGPHEKHVEE+VKYFDYGLWWVILGVASSIGLGSG
Sbjct: 61  ILAKGGWLLLLNLIIGAAGILVLTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG 120

Query: 121 LHTFVLYLGPHIGFFTIKAVQCGRVDLKSAPYDTIQLKRGPSWLEKDCSEFGPPIFTSQV 180
           LHTFVLYLGPHIGFFTIKAVQCGRVDLKSA YDTIQLKRGPSWLEKDC EFGPP+FTSQV
Sbjct: 121 LHTFVLYLGPHIGFFTIKAVQCGRVDLKSAVYDTIQLKRGPSWLEKDCLEFGPPVFTSQV 180

Query: 181 PLSSILPMVQIEAILWGIGTALGELPPYFISRAARLSGGRSEAMEELDASSRETSGLIST 240
           PLSSILPMVQIEAILWGIGT+LGELPPYFISRAARLSGGRSEAMEELDASSRET GLIST
Sbjct: 181 PLSSILPMVQIEAILWGIGTSLGELPPYFISRAARLSGGRSEAMEELDASSRETDGLIST 240

Query: 241 YLNRVKRWFLSHAQHLNFLSILLLASVPNPLFDLAGIMCGQFGIPFWEFFLATLVGKAII 300
           YLNR+KRWFLSHAQHLNFL+ILLLAS+PNPLFDLAGIMCGQFGIPFWEFF ATLVGKAII
Sbjct: 241 YLNRIKRWFLSHAQHLNFLTILLLASIPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII 300

Query: 301 KTHIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSSILPELIAKLNSVKAKYLKAPSHA 360
           KTHIQTIFIIAVCNNQLLDWIENEL+WILSFVPGFSSILP L+AKLN+VK+KYLKAPS  
Sbjct: 301 KTHIQTIFIIAVCNNQLLDWIENELLWILSFVPGFSSILPGLVAKLNAVKSKYLKAPSQV 360

Query: 361 AANAKAKKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDKELAALTNKKVPAS 398
           AANAKAKKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQD+ELAA+T+ KV AS
Sbjct: 361 AANAKAKKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDEELAAMTD-KVSAS 420

BLAST of Sgr011474 vs. NCBI nr
Match: XP_023000445.1 (vacuole membrane protein KMS1-like [Cucurbita maxima])

HSP 1 Score: 699.5 bits (1804), Expect = 1.6e-197
Identity = 360/424 (84.91%), Postives = 383/424 (90.33%), Query Frame = 0

Query: 1   MGSGEEAASIASYASEVSISG----------------------KYSSL-----AKRSIAY 60
           MG+GEEAAS+A++ SEVSIS                       K+ +L      K SIAY
Sbjct: 1   MGTGEEAASLAAHDSEVSISELCRKHKQDLDNLTMVNQPFKTLKFFTLGVLQYGKHSIAY 60

Query: 61  ILAKGGWLLLLNVIIGAAGILILTIEGPHEKHVEEVVKYFDYGLWWVILGVASSIGLGSG 120
           ILAKGGWLLLLN+IIGAAGIL+LTIEGPHEKHVEE+VKYFDYGLWWVILGVASSIGLGSG
Sbjct: 61  ILAKGGWLLLLNLIIGAAGILVLTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG 120

Query: 121 LHTFVLYLGPHIGFFTIKAVQCGRVDLKSAPYDTIQLKRGPSWLEKDCSEFGPPIFTSQV 180
           LHTFVLYLGPHIGFFTIKAVQCGRVDLKSA YDTIQLKRGPSWLEKDCSEFGPP+FTSQV
Sbjct: 121 LHTFVLYLGPHIGFFTIKAVQCGRVDLKSAVYDTIQLKRGPSWLEKDCSEFGPPVFTSQV 180

Query: 181 PLSSILPMVQIEAILWGIGTALGELPPYFISRAARLSGGRSEAMEELDASSRETSGLIST 240
           PLSSILPMVQIEAILWGIGT+LGELPPYFISRAARLSG RSEAMEELDASSRET+GLIST
Sbjct: 181 PLSSILPMVQIEAILWGIGTSLGELPPYFISRAARLSGSRSEAMEELDASSRETNGLIST 240

Query: 241 YLNRVKRWFLSHAQHLNFLSILLLASVPNPLFDLAGIMCGQFGIPFWEFFLATLVGKAII 300
           YLNR+KRWFLSHAQHLNFL+ILLLAS+PNPLFDLAGIMCGQFGIPFWEFF ATLVGKAII
Sbjct: 241 YLNRIKRWFLSHAQHLNFLTILLLASIPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII 300

Query: 301 KTHIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSSILPELIAKLNSVKAKYLKAPSHA 360
           KTHIQTIFIIAVCNNQLL WIENEL+WILSFVPGFSSILP L+AKLN+VK+KYLKAPS  
Sbjct: 301 KTHIQTIFIIAVCNNQLLYWIENELLWILSFVPGFSSILPGLVAKLNAVKSKYLKAPSQV 360

Query: 361 AANAKAKKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDKELAALTNKKVPAS 398
           AANAKAKKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQD+ELAA+T+ KV AS
Sbjct: 361 AANAKAKKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDEELAAMTD-KVSAS 420

BLAST of Sgr011474 vs. ExPASy Swiss-Prot
Match: Q5XF36 (Vacuole membrane protein KMS1 OS=Arabidopsis thaliana OX=3702 GN=KMS1 PE=1 SV=1)

HSP 1 Score: 550.8 bits (1418), Expect = 1.2e-155
Identity = 262/364 (71.98%), Postives = 313/364 (85.99%), Query Frame = 0

Query: 28  KRSIAYILAKGGWLLLLNVIIGAAGILILTIEGPHEKHVEEVVKYFDYGLWWVILGVASS 87
           KRSI+Y+LA GGW +L+  ++  +G L++T++GPH KHVEEV++Y  YGLWW+ LGVASS
Sbjct: 51  KRSISYLLAHGGWFILITTLLVVSGGLLVTVDGPHGKHVEEVLEYVRYGLWWIALGVASS 110

Query: 88  IGLGSGLHTFVLYLGPHIGFFTIKAVQCGRVDLKSAPYDTIQLKRGPSWLEKDCSEFGPP 147
           IGLGSGLHTFVLYLGPHI  FT+KA  CGRVDLKSAPYDTIQLKR PSWL+K CSEFGPP
Sbjct: 111 IGLGSGLHTFVLYLGPHIALFTLKATLCGRVDLKSAPYDTIQLKRVPSWLDKSCSEFGPP 170

Query: 148 IFT----SQVPLSSILPMVQIEAILWGIGTALGELPPYFISRAARLSGGRSEAMEELDAS 207
           +      S+VPL+SILP VQ+EAILWGIGTALGELPPYFISRAA +SG   + MEELD S
Sbjct: 171 LMISAAGSRVPLTSILPQVQLEAILWGIGTALGELPPYFISRAASISGSTVDGMEELDGS 230

Query: 208 SRETSGLISTYLNRVKRWFLSHAQHLNFLSILLLASVPNPLFDLAGIMCGQFGIPFWEFF 267
           S E SG ++T+LNRVKRW L+H+QHLNF ++L+LASVPNPLFDLAGIMCGQFGIPFWEFF
Sbjct: 231 STEDSGFMATHLNRVKRWLLTHSQHLNFFTVLVLASVPNPLFDLAGIMCGQFGIPFWEFF 290

Query: 268 LATLVGKAIIKTHIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSSILPELIAKLNSVK 327
           LATL+GKAIIKTHIQTIFII VCNNQLLDW+ENELIWILS VPG +S+LP L AKL+++K
Sbjct: 291 LATLIGKAIIKTHIQTIFIICVCNNQLLDWMENELIWILSHVPGLASMLPGLTAKLHAMK 350

Query: 328 AKYLKAPSHAAANAKAKKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDKELA 387
            KY+ APS   ++ K KKWDFS +SIWN IVWLM++NF +KI+T+TAQR+LKK+Q+KE+A
Sbjct: 351 EKYIDAPSPVPSHIKVKKWDFSFASIWNGIVWLMLLNFFVKIVTATAQRHLKKKQEKEMA 410

BLAST of Sgr011474 vs. ExPASy Swiss-Prot
Match: F4I8Q7 (Vacuole membrane protein KMS2 OS=Arabidopsis thaliana OX=3702 GN=KMS2 PE=1 SV=1)

HSP 1 Score: 511.5 bits (1316), Expect = 8.4e-144
Identity = 250/361 (69.25%), Postives = 298/361 (82.55%), Query Frame = 0

Query: 28  KRSIAYILAKGGWLLLLNVIIGAAGILILTIEGPHEKHVEEVVKYFDYGLWWVILGVASS 87
           +R  +Y+LA  GWL+L   I  A   L++T++GPH KHVEE+ +Y  +GLWW+ LGVASS
Sbjct: 50  RRWSSYLLANVGWLILFCSIFVAFAALLVTLDGPHVKHVEELSEYTRFGLWWIFLGVASS 109

Query: 88  IGLGSGLHTFVLYLGPHIGFFTIKAVQCGRVDLKSAPYDTIQLKRGPSWLEKDCSEFGPP 147
           IGLGSGLHTFVLYLGPHI  FTIK +QCGRVDLKSA YDTIQLKR PSWL+K C EFG P
Sbjct: 110 IGLGSGLHTFVLYLGPHIALFTIKVMQCGRVDLKSAIYDTIQLKRSPSWLDKPCHEFGSP 169

Query: 148 IFTSQVPLSSILPMVQIEAILWGIGTALGELPPYFISRAARLSGGRSEAMEELDASSRET 207
           +F+S VPLSSILP VQIEAILWG+GTALGELPPYFISRAA LSGG+   M+EL+  S + 
Sbjct: 170 VFSSGVPLSSILPQVQIEAILWGLGTALGELPPYFISRAASLSGGK---MKELETCSGDD 229

Query: 208 SGLISTYLNRVKRWFLSHAQHLNFLSILLLASVPNPLFDLAGIMCGQFGIPFWEFFLATL 267
           +G I+  +N++K W LSH+Q+LNF +IL+LASVPNPLFDLAGIMCGQF  PFWEFFLATL
Sbjct: 230 NGFIAKRVNQIKSWLLSHSQYLNFFTILILASVPNPLFDLAGIMCGQFEKPFWEFFLATL 289

Query: 268 VGKAIIKTHIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSSILPELIAKLNSVKAKYL 327
           +GKAIIKTHIQT+FII VCNNQLLDW+ENELI+ILSFVPGF+S LPEL AKL  +K KYL
Sbjct: 290 IGKAIIKTHIQTVFIICVCNNQLLDWVENELIYILSFVPGFASALPELTAKLRLMKEKYL 349

Query: 328 KAPSHAAANAKAKKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDKELAALTN 387
            A    +++   KKWD S +S+WN +VWLM++NF  +I+TSTAQRYLKKQQ++EL ALTN
Sbjct: 350 IASPPVSSDINVKKWDLSFASVWNGVVWLMLLNFFGQIVTSTAQRYLKKQQEEELDALTN 407

Query: 388 K 389
           K
Sbjct: 410 K 407

BLAST of Sgr011474 vs. ExPASy Swiss-Prot
Match: Q6NYY9 (Vacuole membrane protein 1 OS=Danio rerio OX=7955 GN=vmp1 PE=2 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 7.3e-55
Identity = 129/343 (37.61%), Postives = 195/343 (56.85%), Query Frame = 0

Query: 41  LLLLNVIIGAAGILILTIEGPHEKHVEEVVKYFDYGLWWVILGVASSIGLGSGLHTFVLY 100
           ++ L V++ +   L  +IEG H+++V+ + K F +  +WV LG+ SS+GLG+GLHTF+LY
Sbjct: 78  VVFLTVVLFSLFSLAYSIEGAHQEYVQHLEKKFLWCAYWVGLGILSSVGLGTGLHTFLLY 137

Query: 101 LGPHIGFFTIKAVQCGRVDLKSAPYDTIQLKRGPSWLEKDCSEFGPPIFTSQVPLSSILP 160
           LGPHI   T+ A +CG V+    PY            +  C E         + L +I+ 
Sbjct: 138 LGPHIASVTLAAYECGSVNFPEPPYPA----------QIVCPE--DEALQESISLWTIMS 197

Query: 161 MVQIEAILWGIGTALGELPPYFISRAARLSGG--RSEAMEELDASSRETSGLISTYLNRV 220
            V++EA +WG GTA+GELPPYF++RAAR+SG     E  EE +    E S     + +R 
Sbjct: 198 KVRLEACMWGAGTAIGELPPYFMARAARMSGADPDDEDYEEFE-EMLEHSQSAQDFASRA 257

Query: 221 KRWFLSHAQHLNFLSILLLASVPNPLFDLAGIMCGQFGIPFWEFFLATLVGKAIIKTHIQ 280
           K    +  Q + F  IL  AS+PNPLFDLAGI CG F IPFW FF ATL+GKAIIK HIQ
Sbjct: 258 KLAVQNMVQKVGFFGILACASIPNPLFDLAGITCGHFLIPFWTFFGATLIGKAIIKMHIQ 317

Query: 281 TIFIIAVCNNQLLDWIENELIWILSFVPGFSSILPELIAKLNSVKAKYLKAPSHAAANAK 340
            +F+I   +  +++    +++ ++  +PG  + L +   +    +   L  P+   A A 
Sbjct: 318 KLFVIITFSKHIVE----QMVSLIGVIPGVGASLQKPFREYLEAQRTKLHNPAGDGA-AA 377

Query: 341 AKKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDKE 382
            + W   +S ++  +V +M+  F + I+ S AQ Y K+ Q K+
Sbjct: 378 GESW---LSWVFEKVVLVMVCYFILSIINSMAQSYAKRLQQKK 399

BLAST of Sgr011474 vs. ExPASy Swiss-Prot
Match: Q9XWU8 (Ectopic P granules protein 3 OS=Caenorhabditis elegans OX=6239 GN=epg-3 PE=2 SV=2)

HSP 1 Score: 210.7 bits (535), Expect = 3.0e-53
Identity = 138/364 (37.91%), Postives = 196/364 (53.85%), Query Frame = 0

Query: 34  ILAKGGWLLLLNVIIGAAGILILTIEGPHEKHVEEVVKYFDYGLWWVILGVASSIGLGSG 93
           ILA    LLL  + IG A +      G H++HV+ + K+  +  WWV+LGV SSIGLGSG
Sbjct: 83  ILAHKTVLLLTAISIGLA-VYGYHAPGAHQEHVQTIEKHILWWSWWVLLGVLSSIGLGSG 142

Query: 94  LHTFVLYLGPHIGFFTIKAVQCGRVDLKSAPY-DTIQLKRGPSWLEKDCSEFGPPIFTSQ 153
           LHTF++YLGPHI   T+ A +C  +D    PY ++IQ     S              +  
Sbjct: 143 LHTFLIYLGPHIAAVTMAAYECQSLDFPQPPYPESIQCPSTKS--------------SIA 202

Query: 154 VPLSSILPMVQIEAILWGIGTALGELPPYFISRAARLSGGR------SEAMEELDASSRE 213
           V    I+  V++E++LWG GTALGELPPYF++RAAR+SG         E +E ++A    
Sbjct: 203 VTFWQIVAKVRVESLLWGAGTALGELPPYFMARAARISGQEPDDEEYREFLELMNADKES 262

Query: 214 TSGLISTYLNRVKRWFLSHAQHLNFLSILLLASVPNPLFDLAGIMCGQFGIPFWEFFLAT 273
            +    + + R K W   +   L F  ILL AS+PNPLFDLAGI CG F +PFW FF AT
Sbjct: 263 DADQKLSIVERAKSWVEHNIHRLGFPGILLFASIPNPLFDLAGITCGHFLVPFWSFFGAT 322

Query: 274 LVGKAIIKTHIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSSILPELIAKLNSVKAKY 333
           L+GKA++K H+Q  F+I   +    D      + IL  +P     + + I+ L   + K 
Sbjct: 323 LIGKALVKMHVQMGFVILAFS----DHHAENFVKILEKIPAVGPYIRQPISDLLEKQRKA 382

Query: 334 L-KAP-SHAAANAKAKKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRY------LKKQQ 383
           L K P  H+  +     W  S+      +V  MI+ F + I+ S A+ Y       K++Q
Sbjct: 383 LHKTPGEHSEQSTSYLAWGLSL------MVTFMILFFFLSIVNSLAKDYHKRLWERKRRQ 421

BLAST of Sgr011474 vs. ExPASy Swiss-Prot
Match: Q99KU0 (Vacuole membrane protein 1 OS=Mus musculus OX=10090 GN=Vmp1 PE=1 SV=2)

HSP 1 Score: 209.1 bits (531), Expect = 8.9e-53
Identity = 127/337 (37.69%), Postives = 189/337 (56.08%), Query Frame = 0

Query: 46  VIIGAAGILILTIEGPHEKHVEEVVKYFDYGLWWVILGVASSIGLGSGLHTFVLYLGPHI 105
           +++ AA +    +EG H+++V+ + K F    +W+ LG+ SS+GLG+GLHTF+LYLGPHI
Sbjct: 83  LLLLAALVATYYVEGAHQQYVQRIEKQFLLYAYWIGLGILSSVGLGTGLHTFLLYLGPHI 142

Query: 106 GFFTIKAVQCGRVDLKSAPY-DTIQLKRGPSWLEKDCSEFGPPIFTSQVPLSSILPMVQI 165
              T+ A +C  V+    PY D I         E++ +E         + L SI+  V+I
Sbjct: 143 ASVTLAAYECNSVNFPEPPYPDQIICP------EEEGAE-------GAISLWSIISKVRI 202

Query: 166 EAILWGIGTALGELPPYFISRAARLSGGRSEAMEELDASSR-ETSGLISTYLNRVKRWFL 225
           EA +WGIGTA+GELPPYF++RAARLSG   +  E  +     E +     + +R K    
Sbjct: 203 EACMWGIGTAIGELPPYFMARAARLSGAEPDDEEYQEFEEMLEHAEAAQDFASRAKLAVQ 262

Query: 226 SHAQHLNFLSILLLASVPNPLFDLAGIMCGQFGIPFWEFFLATLVGKAIIKTHIQTIFII 285
              Q + F  IL  AS+PNPLFDLAGI CG F +PFW FF ATL+GKAIIK HIQ IF+I
Sbjct: 263 KLVQKVGFFGILACASIPNPLFDLAGITCGHFLVPFWTFFGATLIGKAIIKMHIQKIFVI 322

Query: 286 AVCNNQLLDWIENELIWILSFVPGFSSILPELIAKLNSVKAKYLKAPSHAAANAKAKKWD 345
              +  +++    +++  +  VPG    L +   +    + + L   S  A   + + W 
Sbjct: 323 VTFSKHIVE----QMVTFIGAVPGIGPSLQKPFQEYLEAQRQKLHHRSE-AGTPQGENW- 382

Query: 346 FSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDK 381
             +S ++  +V  M+  F + I+ S AQ Y K+ Q +
Sbjct: 383 --LSWMFEKLVVAMVCYFVLSIINSMAQNYAKRIQQR 398

BLAST of Sgr011474 vs. ExPASy TrEMBL
Match: A0A6J1ERI3 (vacuole membrane protein KMS1-like OS=Cucurbita moschata OX=3662 GN=LOC111436919 PE=4 SV=1)

HSP 1 Score: 702.6 bits (1812), Expect = 9.4e-199
Identity = 361/424 (85.14%), Postives = 383/424 (90.33%), Query Frame = 0

Query: 1   MGSGEEAASIASYASEVSISG----------------------KYSSL-----AKRSIAY 60
           MG+GEEAAS+A++ SEVSIS                       K+ +L      K SIAY
Sbjct: 1   MGTGEEAASLAAHDSEVSISELCRKHKQDLDNLTLVNQPFKTLKFFTLGVLQYGKHSIAY 60

Query: 61  ILAKGGWLLLLNVIIGAAGILILTIEGPHEKHVEEVVKYFDYGLWWVILGVASSIGLGSG 120
           ILAKGGWLLLLN+IIGAAGIL+LTIEGPHEKHVEE+VKYFDYGLWWVILGVASSIGLGSG
Sbjct: 61  ILAKGGWLLLLNLIIGAAGILVLTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG 120

Query: 121 LHTFVLYLGPHIGFFTIKAVQCGRVDLKSAPYDTIQLKRGPSWLEKDCSEFGPPIFTSQV 180
           LHTFVLYLGPHIGFFTIKAVQCGRVDLKSA YDTIQLKRGPSWLEKDC EFGPP+FTSQV
Sbjct: 121 LHTFVLYLGPHIGFFTIKAVQCGRVDLKSAVYDTIQLKRGPSWLEKDCLEFGPPVFTSQV 180

Query: 181 PLSSILPMVQIEAILWGIGTALGELPPYFISRAARLSGGRSEAMEELDASSRETSGLIST 240
           PLSSILPMVQIEAILWGIGT+LGELPPYFISRAARLSGGRSEAMEELDASSRET GLIST
Sbjct: 181 PLSSILPMVQIEAILWGIGTSLGELPPYFISRAARLSGGRSEAMEELDASSRETDGLIST 240

Query: 241 YLNRVKRWFLSHAQHLNFLSILLLASVPNPLFDLAGIMCGQFGIPFWEFFLATLVGKAII 300
           YLNR+KRWFLSHAQHLNFL+ILLLAS+PNPLFDLAGIMCGQFGIPFWEFF ATLVGKAII
Sbjct: 241 YLNRIKRWFLSHAQHLNFLTILLLASIPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII 300

Query: 301 KTHIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSSILPELIAKLNSVKAKYLKAPSHA 360
           KTHIQTIFIIAVCNNQLLDWIENEL+WILSFVPGFSSILP L+AKLN+VK+KYLKAPS  
Sbjct: 301 KTHIQTIFIIAVCNNQLLDWIENELLWILSFVPGFSSILPGLVAKLNAVKSKYLKAPSQV 360

Query: 361 AANAKAKKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDKELAALTNKKVPAS 398
           AANAKAKKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQD+ELAA+T+ KV AS
Sbjct: 361 AANAKAKKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDEELAAMTD-KVSAS 420

BLAST of Sgr011474 vs. ExPASy TrEMBL
Match: A0A6J1KIC8 (vacuole membrane protein KMS1-like OS=Cucurbita maxima OX=3661 GN=LOC111494711 PE=4 SV=1)

HSP 1 Score: 699.5 bits (1804), Expect = 8.0e-198
Identity = 360/424 (84.91%), Postives = 383/424 (90.33%), Query Frame = 0

Query: 1   MGSGEEAASIASYASEVSISG----------------------KYSSL-----AKRSIAY 60
           MG+GEEAAS+A++ SEVSIS                       K+ +L      K SIAY
Sbjct: 1   MGTGEEAASLAAHDSEVSISELCRKHKQDLDNLTMVNQPFKTLKFFTLGVLQYGKHSIAY 60

Query: 61  ILAKGGWLLLLNVIIGAAGILILTIEGPHEKHVEEVVKYFDYGLWWVILGVASSIGLGSG 120
           ILAKGGWLLLLN+IIGAAGIL+LTIEGPHEKHVEE+VKYFDYGLWWVILGVASSIGLGSG
Sbjct: 61  ILAKGGWLLLLNLIIGAAGILVLTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG 120

Query: 121 LHTFVLYLGPHIGFFTIKAVQCGRVDLKSAPYDTIQLKRGPSWLEKDCSEFGPPIFTSQV 180
           LHTFVLYLGPHIGFFTIKAVQCGRVDLKSA YDTIQLKRGPSWLEKDCSEFGPP+FTSQV
Sbjct: 121 LHTFVLYLGPHIGFFTIKAVQCGRVDLKSAVYDTIQLKRGPSWLEKDCSEFGPPVFTSQV 180

Query: 181 PLSSILPMVQIEAILWGIGTALGELPPYFISRAARLSGGRSEAMEELDASSRETSGLIST 240
           PLSSILPMVQIEAILWGIGT+LGELPPYFISRAARLSG RSEAMEELDASSRET+GLIST
Sbjct: 181 PLSSILPMVQIEAILWGIGTSLGELPPYFISRAARLSGSRSEAMEELDASSRETNGLIST 240

Query: 241 YLNRVKRWFLSHAQHLNFLSILLLASVPNPLFDLAGIMCGQFGIPFWEFFLATLVGKAII 300
           YLNR+KRWFLSHAQHLNFL+ILLLAS+PNPLFDLAGIMCGQFGIPFWEFF ATLVGKAII
Sbjct: 241 YLNRIKRWFLSHAQHLNFLTILLLASIPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII 300

Query: 301 KTHIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSSILPELIAKLNSVKAKYLKAPSHA 360
           KTHIQTIFIIAVCNNQLL WIENEL+WILSFVPGFSSILP L+AKLN+VK+KYLKAPS  
Sbjct: 301 KTHIQTIFIIAVCNNQLLYWIENELLWILSFVPGFSSILPGLVAKLNAVKSKYLKAPSQV 360

Query: 361 AANAKAKKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDKELAALTNKKVPAS 398
           AANAKAKKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQD+ELAA+T+ KV AS
Sbjct: 361 AANAKAKKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDEELAAMTD-KVSAS 420

BLAST of Sgr011474 vs. ExPASy TrEMBL
Match: A0A6J1C9E2 (vacuole membrane protein KMS1-like OS=Momordica charantia OX=3673 GN=LOC111009594 PE=4 SV=1)

HSP 1 Score: 698.4 bits (1801), Expect = 1.8e-197
Identity = 347/371 (93.53%), Postives = 360/371 (97.04%), Query Frame = 0

Query: 27  AKRSIAYILAKGGWLLLLNVIIGAAGILILTIEGPHEKHVEEVVKYFDYGLWWVILGVAS 86
           +K SIAYIL+KGGWLLLLNVIIGAAGILIL IEGPHEKHVEE+VKYFDYGLWWVILGVAS
Sbjct: 54  SKSSIAYILSKGGWLLLLNVIIGAAGILILNIEGPHEKHVEEIVKYFDYGLWWVILGVAS 113

Query: 87  SIGLGSGLHTFVLYLGPHIGFFTIKAVQCGRVDLKSAPYDTIQLKRGPSWLEKDCSEFGP 146
           SIGLGSGLHTFVLYLGPHIGFFTIKAVQCGRVDLKSAPYDTIQLKRGPSWLEKDCSEFGP
Sbjct: 114 SIGLGSGLHTFVLYLGPHIGFFTIKAVQCGRVDLKSAPYDTIQLKRGPSWLEKDCSEFGP 173

Query: 147 PIFTSQVPLSSILPMVQIEAILWGIGTALGELPPYFISRAARLSGGRSEAMEELDASSRE 206
           P+FTS+VPLSSILPMVQ+EAILWGIGTALGELPPYFISRAARLSGGRSEAMEELDASSRE
Sbjct: 174 PVFTSRVPLSSILPMVQLEAILWGIGTALGELPPYFISRAARLSGGRSEAMEELDASSRE 233

Query: 207 TSGLISTYLNRVKRWFLSHAQHLNFLSILLLASVPNPLFDLAGIMCGQFGIPFWEFFLAT 266
           T+G I  +LNR+KRWFLSHAQHLNF++ILLLASVPNPLFDLAGIMCGQFGIPFWEFF AT
Sbjct: 234 TNGFIPKHLNRIKRWFLSHAQHLNFVTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFAT 293

Query: 267 LVGKAIIKTHIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSSILPELIAKLNSVKAKY 326
           LVGKAIIKTHIQTIFIIAVCNNQLLDWIENELIW+LSFVPGFSSILP LIAKLNSVKAKY
Sbjct: 294 LVGKAIIKTHIQTIFIIAVCNNQLLDWIENELIWVLSFVPGFSSILPGLIAKLNSVKAKY 353

Query: 327 LKAPSHAAANAKAKKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDKELAALT 386
           LKAPSHAAAN K KKWDFSVSS WNTIVWLMIMNFSIKIMTSTAQRYLKKQQD+ELAALT
Sbjct: 354 LKAPSHAAANTKDKKWDFSVSSXWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDEELAALT 413

Query: 387 NKKVPASACTN 398
           N KVPASACTN
Sbjct: 414 N-KVPASACTN 423

BLAST of Sgr011474 vs. ExPASy TrEMBL
Match: A0A5A7V7Q6 (Vacuole membrane protein KMS1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold75860G00460 PE=4 SV=1)

HSP 1 Score: 694.5 bits (1791), Expect = 2.6e-196
Identity = 359/423 (84.87%), Postives = 379/423 (89.60%), Query Frame = 0

Query: 1   MGSGEEAASIASYASEVS---------------ISGKYSSL----------AKRSIAYIL 60
           MG+ EEAASIAS+ SEVS               ++  + +L          +KRSIAYIL
Sbjct: 1   MGTVEEAASIASHDSEVSKLRRQQKQELENLTLVNQPFKTLKFFILGVLQYSKRSIAYIL 60

Query: 61  AKGGWLLLLNVIIGAAGILILTIEGPHEKHVEEVVKYFDYGLWWVILGVASSIGLGSGLH 120
           AKGGWLLLLN+IIGAAGILILTIEGPHEKHVEE+VKYFDYGLWWVILGVASSIGLGSGLH
Sbjct: 61  AKGGWLLLLNLIIGAAGILILTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSGLH 120

Query: 121 TFVLYLGPHIGFFTIKAVQCGRVDLKSAPYDTIQLKRGPSWLEKDCSEFGPPIFTSQVPL 180
           TFVLYLGPHIGFFTIKAVQCGRVDLKSA YDTIQLKRGPSWLEKDCSEFGPP+FTS+VPL
Sbjct: 121 TFVLYLGPHIGFFTIKAVQCGRVDLKSATYDTIQLKRGPSWLEKDCSEFGPPLFTSRVPL 180

Query: 181 SSILPMVQIEAILWGIGTALGELPPYFISRAARLSGGRSEAMEELDASSRETSGLISTYL 240
           SSILPMVQ+EAILWGIGTALGELPPYFISRAARLSG RSEAMEELDASSRE++G I TYL
Sbjct: 181 SSILPMVQVEAILWGIGTALGELPPYFISRAARLSGARSEAMEELDASSRESNGFIPTYL 240

Query: 241 NRVKRWFLSHAQHLNFLSILLLASVPNPLFDLAGIMCGQFGIPFWEFFLATLVGKAIIKT 300
           NR+KRWFLSHAQHLNFL+ILLLASVPNPLFDLAGIMCGQFGIPFWEFF ATLVGKAIIKT
Sbjct: 241 NRMKRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAIIKT 300

Query: 301 HIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSSILPELIAKLNSVKAKYLKAPSHA-A 360
           HIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSS LP L+AKLN++KAKYLKAPSHA  
Sbjct: 301 HIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSSALPGLVAKLNAIKAKYLKAPSHAIT 360

Query: 361 ANAKAKKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDKELAALTNKKVPASA 398
            N K KKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDKELAALTN KVPAS 
Sbjct: 361 TNPKVKKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDKELAALTN-KVPAST 420

BLAST of Sgr011474 vs. ExPASy TrEMBL
Match: A0A1S3BMX8 (vacuole membrane protein KMS1 OS=Cucumis melo OX=3656 GN=LOC103491404 PE=4 SV=1)

HSP 1 Score: 694.5 bits (1791), Expect = 2.6e-196
Identity = 359/423 (84.87%), Postives = 379/423 (89.60%), Query Frame = 0

Query: 1   MGSGEEAASIASYASEVS---------------ISGKYSSL----------AKRSIAYIL 60
           MG+ EEAASIAS+ SEVS               ++  + +L          +KRSIAYIL
Sbjct: 1   MGTVEEAASIASHDSEVSKLRRQQKQELENLTLVNQPFKTLKFFILGVLQYSKRSIAYIL 60

Query: 61  AKGGWLLLLNVIIGAAGILILTIEGPHEKHVEEVVKYFDYGLWWVILGVASSIGLGSGLH 120
           AKGGWLLLLN+IIGAAGILILTIEGPHEKHVEE+VKYFDYGLWWVILGVASSIGLGSGLH
Sbjct: 61  AKGGWLLLLNLIIGAAGILILTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSGLH 120

Query: 121 TFVLYLGPHIGFFTIKAVQCGRVDLKSAPYDTIQLKRGPSWLEKDCSEFGPPIFTSQVPL 180
           TFVLYLGPHIGFFTIKAVQCGRVDLKSA YDTIQLKRGPSWLEKDCSEFGPP+FTS+VPL
Sbjct: 121 TFVLYLGPHIGFFTIKAVQCGRVDLKSATYDTIQLKRGPSWLEKDCSEFGPPLFTSRVPL 180

Query: 181 SSILPMVQIEAILWGIGTALGELPPYFISRAARLSGGRSEAMEELDASSRETSGLISTYL 240
           SSILPMVQ+EAILWGIGTALGELPPYFISRAARLSG RSEAMEELDASSRE++G I TYL
Sbjct: 181 SSILPMVQVEAILWGIGTALGELPPYFISRAARLSGARSEAMEELDASSRESNGFIPTYL 240

Query: 241 NRVKRWFLSHAQHLNFLSILLLASVPNPLFDLAGIMCGQFGIPFWEFFLATLVGKAIIKT 300
           NR+KRWFLSHAQHLNFL+ILLLASVPNPLFDLAGIMCGQFGIPFWEFF ATLVGKAIIKT
Sbjct: 241 NRMKRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAIIKT 300

Query: 301 HIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSSILPELIAKLNSVKAKYLKAPSHA-A 360
           HIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSS LP L+AKLN++KAKYLKAPSHA  
Sbjct: 301 HIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSSALPGLVAKLNAIKAKYLKAPSHAIT 360

Query: 361 ANAKAKKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDKELAALTNKKVPASA 398
            N K KKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDKELAALTN KVPAS 
Sbjct: 361 TNPKVKKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDKELAALTN-KVPAST 420

BLAST of Sgr011474 vs. TAIR 10
Match: AT4G14950.3 (SNARE associated Golgi protein family )

HSP 1 Score: 550.8 bits (1418), Expect = 8.8e-157
Identity = 262/364 (71.98%), Postives = 313/364 (85.99%), Query Frame = 0

Query: 28  KRSIAYILAKGGWLLLLNVIIGAAGILILTIEGPHEKHVEEVVKYFDYGLWWVILGVASS 87
           KRSI+Y+LA GGW +L+  ++  +G L++T++GPH KHVEEV++Y  YGLWW+ LGVASS
Sbjct: 41  KRSISYLLAHGGWFILITTLLVVSGGLLVTVDGPHGKHVEEVLEYVRYGLWWIALGVASS 100

Query: 88  IGLGSGLHTFVLYLGPHIGFFTIKAVQCGRVDLKSAPYDTIQLKRGPSWLEKDCSEFGPP 147
           IGLGSGLHTFVLYLGPHI  FT+KA  CGRVDLKSAPYDTIQLKR PSWL+K CSEFGPP
Sbjct: 101 IGLGSGLHTFVLYLGPHIALFTLKATLCGRVDLKSAPYDTIQLKRVPSWLDKSCSEFGPP 160

Query: 148 IFT----SQVPLSSILPMVQIEAILWGIGTALGELPPYFISRAARLSGGRSEAMEELDAS 207
           +      S+VPL+SILP VQ+EAILWGIGTALGELPPYFISRAA +SG   + MEELD S
Sbjct: 161 LMISAAGSRVPLTSILPQVQLEAILWGIGTALGELPPYFISRAASISGSTVDGMEELDGS 220

Query: 208 SRETSGLISTYLNRVKRWFLSHAQHLNFLSILLLASVPNPLFDLAGIMCGQFGIPFWEFF 267
           S E SG ++T+LNRVKRW L+H+QHLNF ++L+LASVPNPLFDLAGIMCGQFGIPFWEFF
Sbjct: 221 STEDSGFMATHLNRVKRWLLTHSQHLNFFTVLVLASVPNPLFDLAGIMCGQFGIPFWEFF 280

Query: 268 LATLVGKAIIKTHIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSSILPELIAKLNSVK 327
           LATL+GKAIIKTHIQTIFII VCNNQLLDW+ENELIWILS VPG +S+LP L AKL+++K
Sbjct: 281 LATLIGKAIIKTHIQTIFIICVCNNQLLDWMENELIWILSHVPGLASMLPGLTAKLHAMK 340

Query: 328 AKYLKAPSHAAANAKAKKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDKELA 387
            KY+ APS   ++ K KKWDFS +SIWN IVWLM++NF +KI+T+TAQR+LKK+Q+KE+A
Sbjct: 341 EKYIDAPSPVPSHIKVKKWDFSFASIWNGIVWLMLLNFFVKIVTATAQRHLKKKQEKEMA 400

BLAST of Sgr011474 vs. TAIR 10
Match: AT4G14950.1 (SNARE associated Golgi protein family )

HSP 1 Score: 550.8 bits (1418), Expect = 8.8e-157
Identity = 262/364 (71.98%), Postives = 313/364 (85.99%), Query Frame = 0

Query: 28  KRSIAYILAKGGWLLLLNVIIGAAGILILTIEGPHEKHVEEVVKYFDYGLWWVILGVASS 87
           KRSI+Y+LA GGW +L+  ++  +G L++T++GPH KHVEEV++Y  YGLWW+ LGVASS
Sbjct: 51  KRSISYLLAHGGWFILITTLLVVSGGLLVTVDGPHGKHVEEVLEYVRYGLWWIALGVASS 110

Query: 88  IGLGSGLHTFVLYLGPHIGFFTIKAVQCGRVDLKSAPYDTIQLKRGPSWLEKDCSEFGPP 147
           IGLGSGLHTFVLYLGPHI  FT+KA  CGRVDLKSAPYDTIQLKR PSWL+K CSEFGPP
Sbjct: 111 IGLGSGLHTFVLYLGPHIALFTLKATLCGRVDLKSAPYDTIQLKRVPSWLDKSCSEFGPP 170

Query: 148 IFT----SQVPLSSILPMVQIEAILWGIGTALGELPPYFISRAARLSGGRSEAMEELDAS 207
           +      S+VPL+SILP VQ+EAILWGIGTALGELPPYFISRAA +SG   + MEELD S
Sbjct: 171 LMISAAGSRVPLTSILPQVQLEAILWGIGTALGELPPYFISRAASISGSTVDGMEELDGS 230

Query: 208 SRETSGLISTYLNRVKRWFLSHAQHLNFLSILLLASVPNPLFDLAGIMCGQFGIPFWEFF 267
           S E SG ++T+LNRVKRW L+H+QHLNF ++L+LASVPNPLFDLAGIMCGQFGIPFWEFF
Sbjct: 231 STEDSGFMATHLNRVKRWLLTHSQHLNFFTVLVLASVPNPLFDLAGIMCGQFGIPFWEFF 290

Query: 268 LATLVGKAIIKTHIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSSILPELIAKLNSVK 327
           LATL+GKAIIKTHIQTIFII VCNNQLLDW+ENELIWILS VPG +S+LP L AKL+++K
Sbjct: 291 LATLIGKAIIKTHIQTIFIICVCNNQLLDWMENELIWILSHVPGLASMLPGLTAKLHAMK 350

Query: 328 AKYLKAPSHAAANAKAKKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDKELA 387
            KY+ APS   ++ K KKWDFS +SIWN IVWLM++NF +KI+T+TAQR+LKK+Q+KE+A
Sbjct: 351 EKYIDAPSPVPSHIKVKKWDFSFASIWNGIVWLMLLNFFVKIVTATAQRHLKKKQEKEMA 410

BLAST of Sgr011474 vs. TAIR 10
Match: AT1G05360.1 (BEST Arabidopsis thaliana protein match is: SNARE associated Golgi protein family (TAIR:AT4G14950.1); Has 332 Blast hits to 326 proteins in 115 species: Archae - 0; Bacteria - 11; Metazoa - 174; Fungi - 0; Plants - 74; Viruses - 0; Other Eukaryotes - 73 (source: NCBI BLink). )

HSP 1 Score: 511.5 bits (1316), Expect = 5.9e-145
Identity = 250/361 (69.25%), Postives = 298/361 (82.55%), Query Frame = 0

Query: 28  KRSIAYILAKGGWLLLLNVIIGAAGILILTIEGPHEKHVEEVVKYFDYGLWWVILGVASS 87
           +R  +Y+LA  GWL+L   I  A   L++T++GPH KHVEE+ +Y  +GLWW+ LGVASS
Sbjct: 50  RRWSSYLLANVGWLILFCSIFVAFAALLVTLDGPHVKHVEELSEYTRFGLWWIFLGVASS 109

Query: 88  IGLGSGLHTFVLYLGPHIGFFTIKAVQCGRVDLKSAPYDTIQLKRGPSWLEKDCSEFGPP 147
           IGLGSGLHTFVLYLGPHI  FTIK +QCGRVDLKSA YDTIQLKR PSWL+K C EFG P
Sbjct: 110 IGLGSGLHTFVLYLGPHIALFTIKVMQCGRVDLKSAIYDTIQLKRSPSWLDKPCHEFGSP 169

Query: 148 IFTSQVPLSSILPMVQIEAILWGIGTALGELPPYFISRAARLSGGRSEAMEELDASSRET 207
           +F+S VPLSSILP VQIEAILWG+GTALGELPPYFISRAA LSGG+   M+EL+  S + 
Sbjct: 170 VFSSGVPLSSILPQVQIEAILWGLGTALGELPPYFISRAASLSGGK---MKELETCSGDD 229

Query: 208 SGLISTYLNRVKRWFLSHAQHLNFLSILLLASVPNPLFDLAGIMCGQFGIPFWEFFLATL 267
           +G I+  +N++K W LSH+Q+LNF +IL+LASVPNPLFDLAGIMCGQF  PFWEFFLATL
Sbjct: 230 NGFIAKRVNQIKSWLLSHSQYLNFFTILILASVPNPLFDLAGIMCGQFEKPFWEFFLATL 289

Query: 268 VGKAIIKTHIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSSILPELIAKLNSVKAKYL 327
           +GKAIIKTHIQT+FII VCNNQLLDW+ENELI+ILSFVPGF+S LPEL AKL  +K KYL
Sbjct: 290 IGKAIIKTHIQTVFIICVCNNQLLDWVENELIYILSFVPGFASALPELTAKLRLMKEKYL 349

Query: 328 KAPSHAAANAKAKKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDKELAALTN 387
            A    +++   KKWD S +S+WN +VWLM++NF  +I+TSTAQRYLKKQQ++EL ALTN
Sbjct: 350 IASPPVSSDINVKKWDLSFASVWNGVVWLMLLNFFGQIVTSTAQRYLKKQQEEELDALTN 407

Query: 388 K 389
           K
Sbjct: 410 K 407

BLAST of Sgr011474 vs. TAIR 10
Match: AT4G14950.2 (SNARE associated Golgi protein family )

HSP 1 Score: 481.1 bits (1237), Expect = 8.6e-136
Identity = 229/315 (72.70%), Postives = 269/315 (85.40%), Query Frame = 0

Query: 28  KRSIAYILAKGGWLLLLNVIIGAAGILILTIEGPHEKHVEEVVKYFDYGLWWVILGVASS 87
           KRSI+Y+LA GGW +L+  ++  +G L++T++GPH KHVEEV++Y  YGLWW+ LGVASS
Sbjct: 41  KRSISYLLAHGGWFILITTLLVVSGGLLVTVDGPHGKHVEEVLEYVRYGLWWIALGVASS 100

Query: 88  IGLGSGLHTFVLYLGPHIGFFTIKAVQCGRVDLKSAPYDTIQLKRGPSWLEKDCSEFGPP 147
           IGLGSGLHTFVLYLGPHI  FT+KA  CGRVDLKSAPYDTIQLKR PSWL+K CSEFGPP
Sbjct: 101 IGLGSGLHTFVLYLGPHIALFTLKATLCGRVDLKSAPYDTIQLKRVPSWLDKSCSEFGPP 160

Query: 148 IFT----SQVPLSSILPMVQIEAILWGIGTALGELPPYFISRAARLSGGRSEAMEELDAS 207
           +      S+VPL+SILP VQ+EAILWGIGTALGELPPYFISRAA +SG   + MEELD S
Sbjct: 161 LMISAAGSRVPLTSILPQVQLEAILWGIGTALGELPPYFISRAASISGSTVDGMEELDGS 220

Query: 208 SRETSGLISTYLNRVKRWFLSHAQHLNFLSILLLASVPNPLFDLAGIMCGQFGIPFWEFF 267
           S E SG ++T+LNRVKRW L+H+QHLNF ++L+LASVPNPLFDLAGIMCGQFGIPFWEFF
Sbjct: 221 STEDSGFMATHLNRVKRWLLTHSQHLNFFTVLVLASVPNPLFDLAGIMCGQFGIPFWEFF 280

Query: 268 LATLVGKAIIKTHIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSSILPELIAKLNSVK 327
           LATL+GKAIIKTHIQTIFII VCNNQLLDW+ENELIWILS VPG +S+LP L AKL+++K
Sbjct: 281 LATLIGKAIIKTHIQTIFIICVCNNQLLDWMENELIWILSHVPGLASMLPGLTAKLHAMK 340

Query: 328 AKYLKAPSHAAANAK 339
            KY+ APS   ++ K
Sbjct: 341 EKYIDAPSPVPSHIK 355