Sgr007683 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr007683
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description2-oxoglutarate-dependent dioxygenase AOP3-like
Locationtig00005930: 14306 .. 16817 (-)
RNA-Seq ExpressionSgr007683
SyntenySgr007683
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGATTCATCGAAAATTTACAACTTCATTTTCATCCCCTCGAATGCCCATCATCTGTTCCATCGTCTGTCACTGTTCAAACAAACTTAAACTTATAAAAGACTCAAACGGGCAGAGATAAAGCTCACAGAACACTGCAGCAATAAGCTCTAAGCCATGGGTTTCAATCTTTAGCGAAGCCCCAGCTTCTTCAGCTTCCTGTTATCAACTTCTCCATTGAAAACCTTGGTCCTGAAAGCAGCAACTGGTTCGAAACATGCAGCCATGTGAGGGCTGCTCTTGAGGAATATGGGTGTTTTGTGGCTGAGTATAATCTCGCCCCACCAGAGCTTGACGACGCCATCTTTAAGGGTTTGGAAGAGCTGTTTGATTTGCCCACAGAAACTAAAATGAAAAACGTTTCTGATAAGCCTTACCATGGATTTTTGGACCATCGGGTCCCCTTTGTTCCTCCTCTCCATGAAAGCTGTGGCATTGAAAATGCAGCAACTTTTGAAGCTGTTGAGTCTTTCGTCAAGCTCATGTGGCCTTCTGGAAATGATCGTTTCTGGTATATATATATTTTTTCTACCTGAATTAATATTTTTCTCTTTGGTTTTTAAATATCATTCAATCCCATTGAATCAATGATCAAATCTAAGTTATGTTTGATAGAAGCTTTTGCATTTAGTTTTCTTTAAGAATCAGGTTGTTTACTCATTCATTGTTTTTACTTTCCTTCTAGTTTTTCAAAATACTTTTGAAATCTTAAGACTTGTTGGGAGATTTTTTTGTTGCGAGATTTTTTTAAGAATATATTGAAGATATTAAATTTAAGGACTCAAACAATACAAGCATGAAAGTTTATGGTCAAGACAAAATGTGTTTTCTTATTAATTACTGGTTTAAATTCTATTTTGGGATACTAAACTTAAAAAAAACAGAAAACAAAAAGAATTGACAAATCGGAAAGAGGTGGTAGTTCTTTTTCTTGTTCTTAAAAGGTGTTACCTAGTAAAATAAATCCTTTTGTTCATACCTGGGCTTCTCATGTACGAAATGAGTCACTTCGAATAAGTTTATTGCTCCGGTTTGGAATACAATTAATCCATGGAAGCAAGAAGAGAGAATTGCAAATTAACGTTATTGAATGGGCCTTAATTTTTCGTGGGCAACTTTTGAATTTGATAGAGGAAAAATAAAAATGGGTTAATGTTTCTATAAGGATTTCCCACCTGCCCTTTAGCAATATATTATTTTTCTTTTATTTCCCTTGTGGGGTGAAAAATTGGATTCTCTGTTGGACAGTGAAAATGTATTTTCATACTCAAAGATGGTATCAGAGATGGAGCAATTAGTAAAGAGGATGGTGTTTGAAAGCTATGGAGTGGAAAAATACTTTGATTCTCACATTGCTTCAACCACTTACCTCCTAAGAACCATGAAATACAGAATCCCTCAGATGGGTGAAGAAAACATTGGTGCAGATGCTCACACAGATAAGAGTTTCTTCACCATTCTTCATCAAAATGGAGTCAATGGCTTGCAAATCAAAACAAAAAATGGTGATAATTGGCTTGGAGTTCAATTCTCTCCAACTTCCTTCTTGGTCATGGCTGGAGATGCATGTTTGGTATACCTCTTTATTATTTTTTTTTACATAGATTTTTTTTTTGAGTCCAACAAGCGTAGGATAGGAGATCGAACCTTCAACTTCAAGAAAGGTAATTGGTGTCTTATCCATTCAGCTATCGAATTGGTTTTGGTTTTTTTATTACATAGACTTAATATTATTTTAGTATCTAAATTTTTATAGTTTAGTCCTTTAAAAGGTTGTGTTTTGATTTGGAAACTTTCATAAAATCCTTAAATTTTAATACCGTATTTAACATAATTAATATTAAGCCACATGTTTTTGAATTAGTTTTTAACCTAAAACTAAAAAAAGTGATGAAACGAATCTATTGTCTTTAATATTTTATCCACTTTCTTTCTTTGAACAAAAATTTGACCTTATATTTTCTCAATTTTATGTGTAAGTAGTGTATAATGTATCTATATAAATTGTCATATCTATGCCATAAAAACTATTAATTATACATAGATGTTAATCAATAACAAAATCGATCATGTCATTTTTTCGCTATTTCTTGACTCGAATTTTAACCATAAGACCTAAATTTAAAATTTATTATAACTTGAGAGGAACTAAATAGAAATTGTTAAATTTGAGGGATCAAATTAAAACGGAGAGTAAATTTTTTTTTTTTTTTTTGGTGGCATGGATAGGCATGGAGCAATGGCAGAATCCACTCAGCCACCCATCGAGTTATCATAGAGGGAAGCAAAGAGAGATACTCGACTGCCCTATTTTCATACCACAAAGGAATTATTGAGATTCCACATGAGTTGGTGGATGAGCAACATCCCTTGAGATTCAACCCATTTAACCATTATGGATTACTTGGTTATTTTTCCACAGATGAGTGTGACAAAAATCCTTCCACTGCCAAAGCTTATTGTGGAGTTTGA

mRNA sequence

ATGGGATTCATCGAAAATTTACAACTTCATTTTCATCCCCTCGAATGCCCATCATCTGTTCCATCGTCTGTCACTCCCCAGCTTCTTCAGCTTCCTGTTATCAACTTCTCCATTGAAAACCTTGGTCCTGAAAGCAGCAACTGGTTCGAAACATGCAGCCATGTGAGGGCTGCTCTTGAGGAATATGGGTGTTTTGTGGCTGAGTATAATCTCGCCCCACCAGAGCTTGACGACGCCATCTTTAAGGGTTTGGAAGAGCTGTTTGATTTGCCCACAGAAACTAAAATGAAAAACGTTTCTGATAAGCCTTACCATGGATTTTTGGACCATCGGGTCCCCTTTGTTCCTCCTCTCCATGAAAGCTGTGGCATTGAAAATGCAGCAACTTTTGAAGCTGTTGAGTCTTTCGTCAAGCTCATGTGGCCTTCTGGAAATGATCGTTTCTGTGAAAATGTATTTTCATACTCAAAGATGGTATCAGAGATGGAGCAATTAGTAAAGAGGATGGTGTTTGAAAGCTATGGAGTGGAAAAATACTTTGATTCTCACATTGCTTCAACCACTTACCTCCTAAGAACCATGAAATACAGAATCCCTCAGATGGGTGAAGAAAACATTGGTGCAGATGCTCACACAGATAAGAGTTTCTTCACCATTCTTCATCAAAATGGAGTCAATGGCTTGCAAATCAAAACAAAAAATGGTGATAATTGGCTTGGAGTTCAATTCTCTCCAACTTCCTTCTTGGTCATGGCTGGAGATGCATGTTTGGCATGGAGCAATGGCAGAATCCACTCAGCCACCCATCGAGTTATCATAGAGGGAAGCAAAGAGAGATACTCGACTGCCCTATTTTCATACCACAAAGGAATTATTGAGATTCCACATGAGTTGGTGGATGAGCAACATCCCTTGAGATTCAACCCATTTAACCATTATGGATTACTTGGTTATTTTTCCACAGATGAGTGTGACAAAAATCCTTCCACTGCCAAAGCTTATTGTGGAGTTTGA

Coding sequence (CDS)

ATGGGATTCATCGAAAATTTACAACTTCATTTTCATCCCCTCGAATGCCCATCATCTGTTCCATCGTCTGTCACTCCCCAGCTTCTTCAGCTTCCTGTTATCAACTTCTCCATTGAAAACCTTGGTCCTGAAAGCAGCAACTGGTTCGAAACATGCAGCCATGTGAGGGCTGCTCTTGAGGAATATGGGTGTTTTGTGGCTGAGTATAATCTCGCCCCACCAGAGCTTGACGACGCCATCTTTAAGGGTTTGGAAGAGCTGTTTGATTTGCCCACAGAAACTAAAATGAAAAACGTTTCTGATAAGCCTTACCATGGATTTTTGGACCATCGGGTCCCCTTTGTTCCTCCTCTCCATGAAAGCTGTGGCATTGAAAATGCAGCAACTTTTGAAGCTGTTGAGTCTTTCGTCAAGCTCATGTGGCCTTCTGGAAATGATCGTTTCTGTGAAAATGTATTTTCATACTCAAAGATGGTATCAGAGATGGAGCAATTAGTAAAGAGGATGGTGTTTGAAAGCTATGGAGTGGAAAAATACTTTGATTCTCACATTGCTTCAACCACTTACCTCCTAAGAACCATGAAATACAGAATCCCTCAGATGGGTGAAGAAAACATTGGTGCAGATGCTCACACAGATAAGAGTTTCTTCACCATTCTTCATCAAAATGGAGTCAATGGCTTGCAAATCAAAACAAAAAATGGTGATAATTGGCTTGGAGTTCAATTCTCTCCAACTTCCTTCTTGGTCATGGCTGGAGATGCATGTTTGGCATGGAGCAATGGCAGAATCCACTCAGCCACCCATCGAGTTATCATAGAGGGAAGCAAAGAGAGATACTCGACTGCCCTATTTTCATACCACAAAGGAATTATTGAGATTCCACATGAGTTGGTGGATGAGCAACATCCCTTGAGATTCAACCCATTTAACCATTATGGATTACTTGGTTATTTTTCCACAGATGAGTGTGACAAAAATCCTTCCACTGCCAAAGCTTATTGTGGAGTTTGA

Protein sequence

MGFIENLQLHFHPLECPSSVPSSVTPQLLQLPVINFSIENLGPESSNWFETCSHVRAALEEYGCFVAEYNLAPPELDDAIFKGLEELFDLPTETKMKNVSDKPYHGFLDHRVPFVPPLHESCGIENAATFEAVESFVKLMWPSGNDRFCENVFSYSKMVSEMEQLVKRMVFESYGVEKYFDSHIASTTYLLRTMKYRIPQMGEENIGADAHTDKSFFTILHQNGVNGLQIKTKNGDNWLGVQFSPTSFLVMAGDACLAWSNGRIHSATHRVIIEGSKERYSTALFSYHKGIIEIPHELVDEQHPLRFNPFNHYGLLGYFSTDECDKNPSTAKAYCGV
Homology
BLAST of Sgr007683 vs. NCBI nr
Match: XP_022153580.1 (2-oxoglutarate-dependent dioxygenase AOP3-like [Momordica charantia])

HSP 1 Score: 551.2 bits (1419), Expect = 6.2e-153
Identity = 257/308 (83.44%), Postives = 283/308 (91.88%), Query Frame = 0

Query: 30  QLPVINFSIENLGPESSNWFETCSHVRAALEEYGCFVAEYNLAPPELDDAIFKGLEELFD 89
           +LPVIN SIEN+ PES+NW E CSH+RAALEEYGCF+ E+  A PELDD IFKGLEELFD
Sbjct: 11  KLPVINLSIENVSPESTNWLEICSHLRAALEEYGCFLGEFGSATPELDDEIFKGLEELFD 70

Query: 90  LPTETKMKNVSDKPYHGFLDHRVPFVPPLHESCGIENAATFEAVESFVKLMWPSGNDRFC 149
           LP ETKMKNVSDKPYHGFLDHR PFV PLH+S GIEN+ +FEAV+SFV LMWPSGN RFC
Sbjct: 71  LPLETKMKNVSDKPYHGFLDHRTPFVLPLHQSFGIENSESFEAVQSFVNLMWPSGNRRFC 130

Query: 150 ENVFSYSKMVSEMEQLVKRMVFESYGVEKYFDSHIASTTYLLRTMKYRIPQMGEENIGAD 209
           ENVFSY+KMVSEME+LVK MVFESYGV+KY+DSHIASTTYLLRTMKYRIP+MGEENIGAD
Sbjct: 131 ENVFSYTKMVSEMEKLVKVMVFESYGVKKYYDSHIASTTYLLRTMKYRIPEMGEENIGAD 190

Query: 210 AHTDKSFFTILHQNGVNGLQIKTKNGDNWLGVQFSPTSFLVMAGDACLAWSNGRIHSATH 269
           AHTDKSFFTILHQN VNGL+IKTK+G +WLGV+F+P SFLVMAGDACLAWSNGRIHSATH
Sbjct: 191 AHTDKSFFTILHQNEVNGLEIKTKDG-HWLGVEFTPNSFLVMAGDACLAWSNGRIHSATH 250

Query: 270 RVIIEGSKERYSTALFSYHKGIIEIPHELVDEQHPLRFNPFNHYGLLGYFSTDECDKNPS 329
           RV+IEG+KERYSTALFSYHKGIIEIP+ELVDE+HPLRF+PFNHYGLL YFST +  K PS
Sbjct: 251 RVMIEGTKERYSTALFSYHKGIIEIPYELVDEKHPLRFHPFNHYGLLAYFSTHDSHKMPS 310

Query: 330 TAKAYCGV 338
           TAKAYCGV
Sbjct: 311 TAKAYCGV 317

BLAST of Sgr007683 vs. NCBI nr
Match: XP_038878200.1 (2-oxoglutarate-dependent dioxygenase AOP3-like [Benincasa hispida])

HSP 1 Score: 550.8 bits (1418), Expect = 8.0e-153
Identity = 260/312 (83.33%), Postives = 282/312 (90.38%), Query Frame = 0

Query: 30  QLPVINFSIEN---LGPESSNWFETCSHVRAALEEYGCFVAEYNLAPPELDDAIFKGLEE 89
           ++PVI FSIEN   L P+S+ W ETCS VRAALEEYGCF+AEY  A  +L D IFKGL+E
Sbjct: 10  EVPVIKFSIENDEDLHPQSTKWLETCSQVRAALEEYGCFLAEYESASSKLYDEIFKGLKE 69

Query: 90  LFDLPTETKMKNVSDKPYHGFLDHRVPFVPPLHESCGIENAATFEAVESFVKLMWPSGND 149
           LFDLP ETKMKNVSDKPYHGFLDH  PFV PLH+S GIENA +F+AVESF+KL+WPSGN 
Sbjct: 70  LFDLPLETKMKNVSDKPYHGFLDHTAPFVLPLHQSLGIENATSFQAVESFLKLLWPSGNH 129

Query: 150 RFCENVFSYSKMVSEMEQLVKRMVFESYGVEKYFDSHIASTTYLLRTMKYRIPQMGEENI 209
            FCEN+FSYS+M+SEMEQLVK+MVFESYGV KY+DSHIASTTYLLRTMKYRIPQMGEENI
Sbjct: 130 HFCENLFSYSEMISEMEQLVKKMVFESYGVGKYYDSHIASTTYLLRTMKYRIPQMGEENI 189

Query: 210 GADAHTDKSFFTILHQ-NGVNGLQIKTKNGDNWLGVQFSPTSFLVMAGDACLAWSNGRIH 269
           GA+AHTDKSFFTILHQ NGVNGL+IK KNGD+WL +QFSP SFLVMAGDACLAWSNGRIH
Sbjct: 190 GAEAHTDKSFFTILHQINGVNGLEIKPKNGDHWLRLQFSPNSFLVMAGDACLAWSNGRIH 249

Query: 270 SATHRVIIEGSKERYSTALFSYHKGIIEIPHELVDEQHPLRFNPFNHYGLLGYFSTDECD 329
           SATHRVIIEGSKERYSTALFSYHKGIIEIP ELVDEQHPLRFNPFNHYGLLGYFSTDEC+
Sbjct: 250 SATHRVIIEGSKERYSTALFSYHKGIIEIPQELVDEQHPLRFNPFNHYGLLGYFSTDECE 309

Query: 330 KNPSTAKAYCGV 338
           K PSTA AYCGV
Sbjct: 310 KMPSTANAYCGV 321

BLAST of Sgr007683 vs. NCBI nr
Match: XP_023525673.1 (2-oxoglutarate-dependent dioxygenase AOP3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 549.3 bits (1414), Expect = 2.3e-152
Identity = 258/319 (80.88%), Postives = 286/319 (89.66%), Query Frame = 0

Query: 22  SSVTPQLLQLPVINFSIENLGPESSNWFETCSHVRAALEEYGCFVAEYN---LAPPELDD 81
           SS  P   ++PVI+FSIENL P+S+ W ETCSHVRAALEEYGCF+AEY        ELDD
Sbjct: 5   SSTNP---EVPVISFSIENLRPKSTKWVETCSHVRAALEEYGCFLAEYENDVEQEVELDD 64

Query: 82  AIFKGLEELFDLPTETKMKNVSDKPYHGFLDHRVPFVPPLHESCGIENAATFEAVESFVK 141
           AIF GLE+LFDLP ETKMKNVSDKPYHGFLDHR PFV PLHES GIENAA+FE VESFVK
Sbjct: 65  AIFNGLEKLFDLPLETKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVK 124

Query: 142 LMWPSGNDRFCENVFSYSKMVSEMEQLVKRMVFESYGVEKYFDSHIASTTYLLRTMKYRI 201
           LMWPSGN  FCE++F+Y+KMVSEME+LVKRMVFESYGVEKY+DSHIASTTYLLR MKYRI
Sbjct: 125 LMWPSGNHHFCEDLFAYTKMVSEMEKLVKRMVFESYGVEKYYDSHIASTTYLLRGMKYRI 184

Query: 202 PQMGEENIGADAHTDKSFFTILHQNGVNGLQIKTKNGDNWLGVQFSPTSFLVMAGDACLA 261
           P+MGE+NIGA+AHTDKSFFTILHQNGVNGL+IK K  D+W+G+QF+P SFLVMAGDACLA
Sbjct: 185 PEMGEQNIGAEAHTDKSFFTILHQNGVNGLEIKAKKDDHWIGLQFNPNSFLVMAGDACLA 244

Query: 262 WSNGRIHSATHRVIIEGSKERYSTALFSYHKGIIEIPHELVDEQHPLRFNPFNHYGLLGY 321
           WSNGRIHSATHRVIIEGSKERYSTALFSYHKG+IEIP+ELVDEQ PL+FNPFNHYGLLGY
Sbjct: 245 WSNGRIHSATHRVIIEGSKERYSTALFSYHKGVIEIPNELVDEQFPLKFNPFNHYGLLGY 304

Query: 322 FSTDECDKNPSTAKAYCGV 338
           FS ++C+K PSTA AYCGV
Sbjct: 305 FSKEDCEKTPSTAGAYCGV 320

BLAST of Sgr007683 vs. NCBI nr
Match: XP_022948698.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita moschata])

HSP 1 Score: 547.7 bits (1410), Expect = 6.8e-152
Identity = 254/311 (81.67%), Postives = 282/311 (90.68%), Query Frame = 0

Query: 30  QLPVINFSIENLGPESSNWFETCSHVRAALEEYGCFVAEYN---LAPPELDDAIFKGLEE 89
           ++PVINFSIENLGP+S+ W ETC+HVRAALEEYGCF+AEY        ELDDAIF GLE+
Sbjct: 10  EVPVINFSIENLGPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELDDAIFNGLEK 69

Query: 90  LFDLPTETKMKNVSDKPYHGFLDHRVPFVPPLHESCGIENAATFEAVESFVKLMWPSGND 149
           LFDLP +TKMKNVSDKPYHGFLDHR PFV PLHES GIENAA+FE VESFVKLMWPSGN 
Sbjct: 70  LFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVKLMWPSGNH 129

Query: 150 RFCENVFSYSKMVSEMEQLVKRMVFESYGVEKYFDSHIASTTYLLRTMKYRIPQMGEENI 209
            FC+++F+Y+KMVSEME+LVKRMVFESYGVEKY+DSHIASTTYLLR MKYRIP+MGE+NI
Sbjct: 130 HFCQDLFAYTKMVSEMEKLVKRMVFESYGVEKYYDSHIASTTYLLRGMKYRIPEMGEQNI 189

Query: 210 GADAHTDKSFFTILHQNGVNGLQIKTKNGDNWLGVQFSPTSFLVMAGDACLAWSNGRIHS 269
           GA+AHTDKSFFTILHQNGVNGL+IK K  D+W+G+QF+  SFLVMAGDACLAWSNGRIHS
Sbjct: 190 GAEAHTDKSFFTILHQNGVNGLEIKAKKDDHWIGLQFNSNSFLVMAGDACLAWSNGRIHS 249

Query: 270 ATHRVIIEGSKERYSTALFSYHKGIIEIPHELVDEQHPLRFNPFNHYGLLGYFSTDECDK 329
           ATHRVIIEGSKERYSTALFSYHKG+IEIP ELVDEQ PL+FNPFNHYGLLGYFS +EC+K
Sbjct: 250 ATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLGYFSKEECEK 309

Query: 330 NPSTAKAYCGV 338
            PSTA AYCGV
Sbjct: 310 TPSTAGAYCGV 320

BLAST of Sgr007683 vs. NCBI nr
Match: XP_022997882.1 (2-oxoglutarate-dependent dioxygenase AOP3-like [Cucurbita maxima])

HSP 1 Score: 546.6 bits (1407), Expect = 1.5e-151
Identity = 254/308 (82.47%), Postives = 280/308 (90.91%), Query Frame = 0

Query: 30  QLPVINFSIENLGPESSNWFETCSHVRAALEEYGCFVAEYNLAPPELDDAIFKGLEELFD 89
           ++P INFSIENL P+S+ W ETCSHVRAALEEYGCF+AE      ELDDAIF GLE+LFD
Sbjct: 10  EVPAINFSIENLRPKSTKWVETCSHVRAALEEYGCFLAECE-NNVELDDAIFNGLEKLFD 69

Query: 90  LPTETKMKNVSDKPYHGFLDHRVPFVPPLHESCGIENAATFEAVESFVKLMWPSGNDRFC 149
           LP ETKMKNVSDKPYHGFLDHR PFV PLHES GIENAA+FE VESFVKLMWPSGN  FC
Sbjct: 70  LPLETKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVKLMWPSGNHHFC 129

Query: 150 ENVFSYSKMVSEMEQLVKRMVFESYGVEKYFDSHIASTTYLLRTMKYRIPQMGEENIGAD 209
           E++F+Y+KMVSEME+LVKRMVFESYGVEKY+DSHIASTTYLLR MKYRIP+MGE+NIGA+
Sbjct: 130 EDLFAYTKMVSEMEKLVKRMVFESYGVEKYYDSHIASTTYLLRGMKYRIPEMGEQNIGAE 189

Query: 210 AHTDKSFFTILHQNGVNGLQIKTKNGDNWLGVQFSPTSFLVMAGDACLAWSNGRIHSATH 269
           AHTDKSFFTILHQNGVNGL+IK K  D+W+G+QF+P SFLVMAGDACLAWSNGRIHSATH
Sbjct: 190 AHTDKSFFTILHQNGVNGLEIKAKKDDHWIGLQFNPNSFLVMAGDACLAWSNGRIHSATH 249

Query: 270 RVIIEGSKERYSTALFSYHKGIIEIPHELVDEQHPLRFNPFNHYGLLGYFSTDECDKNPS 329
           RV+IEGSKERYSTALFSYHKG+IEIP ELVDEQ PL+FNPFNHYGLLGYFS +EC+K PS
Sbjct: 250 RVLIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLGYFSKEECEKTPS 309

Query: 330 TAKAYCGV 338
           TA AYCGV
Sbjct: 310 TAGAYCGV 316

BLAST of Sgr007683 vs. ExPASy Swiss-Prot
Match: Q9ZTA3 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 273.9 bits (699), Expect = 2.5e-72
Identity = 142/312 (45.51%), Postives = 196/312 (62.82%), Query Frame = 0

Query: 20  VPSSVTPQLLQLPVINFSIENLGPESSNWFETCSHVRAALEEYGCFVAEYNLAPPELDDA 79
           VP SV+    QLPVI+FS +NL P SS W E  + V  ALE+YGCF A ++    EL+ +
Sbjct: 6   VPPSVS---FQLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRS 65

Query: 80  IFKGLEELFDLPTETKMKNVSDKPYHGFLDHRVPFVPPLHESCGIENAATFEAVESFVKL 139
           +F+ +E+LF+LP  TK +NVS KP+HG+L H       L+ES GI +A   E V  F + 
Sbjct: 66  VFEAMEDLFELPIPTKQRNVSSKPFHGYLCHN------LYESLGINDANVLEKVNDFTQQ 125

Query: 140 MWPS-GNDRFCENVFSYSKMVSEMEQLVKRMVFESYGVEKYFDSHIASTTYLLRTMKYRI 199
           +WP  GN    E +  +S+ + E++ +V+RM+ ES+G+E Y D H+ ST YL R MKY  
Sbjct: 126 LWPDHGNKSISETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTS 185

Query: 200 PQMGEEN------IGADAHTDKSFFTILHQNGVNGLQIKTKNGDNWLGVQFSPTSFLVMA 259
           P   +++      +G  +HTDK+  TILHQ  V+GL++KTK+ D W+ V+ S  S LVM 
Sbjct: 186 PPDDDDDDDEETKLGLRSHTDKNIITILHQYQVDGLEVKTKD-DKWIKVKPSQDSVLVMV 245

Query: 260 GDACLAWSNGRIHSATHRVIIEGSKERYSTALFSYHKG--IIEIPHELVDEQHPLRFNPF 319
           GD+  A  NGR+HS  HRVI+ G K RYST LFS  K   II+ P ELVD++HP  F PF
Sbjct: 246 GDSLCALLNGRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPF 305

Query: 320 NHYGLLGYFSTD 323
            +   L +F T+
Sbjct: 306 EYTDFLHFFQTE 307

BLAST of Sgr007683 vs. ExPASy Swiss-Prot
Match: Q945B6 (Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=3702 GN=AOP1.2 PE=2 SV=1)

HSP 1 Score: 273.1 bits (697), Expect = 4.2e-72
Identity = 142/311 (45.66%), Postives = 197/311 (63.34%), Query Frame = 0

Query: 20  VPSSVTPQLLQLPVINFSIENLGPESSNWFETCSHVRAALEEYGCFVAEYNLAPPELDDA 79
           VP SV+    QLPVI+FS +NL P SS W E  + V  ALE+YGCF A ++    EL+ +
Sbjct: 6   VPPSVS---FQLPVIDFSDQNLKPGSSKWDEVKADVLKALEDYGCFEAFFDKLSVELNRS 65

Query: 80  IFKGLEELFDLPTETKMKNVSDKPYHGFLDHRVPFVPPLHESCGIENAATFEAVESFVKL 139
           +F+ +E+LF+LP  TK +NVS KP+HG+L H       L+ES GI++A   E V  F + 
Sbjct: 66  VFEAMEDLFELPIPTKQRNVSSKPFHGYLCHN------LYESLGIDDANVLEKVNDFTQQ 125

Query: 140 MWPS-GNDRFCENVFSYSKMVSEMEQLVKRMVFESYGVEKYFDSHIASTTYLLRTMKYRI 199
           +WP  GN    E +  +S+ + E++ +V+RM+ ES+G+E Y D H+ ST YL R MKY  
Sbjct: 126 LWPDHGNKSISETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTS 185

Query: 200 PQMGEEN-----IGADAHTDKSFFTILHQNGVNGLQIKTKNGDNWLGVQFSPTSFLVMAG 259
           P   +++     +G  +HTDK+  TILHQ  V+GL++KTK+ D W+ V+ S  S LVM G
Sbjct: 186 PPDDDDDDEETKLGLRSHTDKNIITILHQYQVDGLEVKTKD-DKWIKVKPSQDSVLVMVG 245

Query: 260 DACLAWSNGRIHSATHRVIIEGSKERYSTALFSYHKG--IIEIPHELVDEQHPLRFNPFN 319
           D+  A  NGR+HS  HRVI+ G K RYST LFS  K   II+ P ELVD++HP  F PF 
Sbjct: 246 DSLCALLNGRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFE 305

Query: 320 HYGLLGYFSTD 323
           +   L +F T+
Sbjct: 306 YTDFLHFFQTE 306

BLAST of Sgr007683 vs. ExPASy Swiss-Prot
Match: Q944Z5 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 270.8 bits (691), Expect = 2.1e-71
Identity = 138/300 (46.00%), Postives = 191/300 (63.67%), Query Frame = 0

Query: 30  QLPVINFSIENLGPESSNWFETCSHVRAALEEYGCFVAEYNLAPPELDDAIFKGLEELFD 89
           QLPVI+FS +NL P SS W E  + V  ALE+YGCF A ++    EL+ ++F+ +E+LF+
Sbjct: 6   QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLFE 65

Query: 90  LPTETKMKNVSDKPYHGFLDHRVPFVPPLHESCGIENAATFEAVESFVKLMWPS-GNDRF 149
           LP  TK +NVS K +HG+L H       L+ES GI +A   E V  F + +WP  GN   
Sbjct: 66  LPIPTKQRNVSSKLFHGYLCHN------LYESLGINDANVLEKVNDFTQQLWPDHGNKSI 125

Query: 150 CENVFSYSKMVSEMEQLVKRMVFESYGVEKYFDSHIASTTYLLRTMKYRIPQMGEEN--- 209
            E +  +S+ + E++ +V+RM+ ES+G+EKY D H+ ST YL R MKY  P   +++   
Sbjct: 126 SETIHLFSEQLVELDLMVRRMIMESFGIEKYIDEHLNSTYYLTRLMKYTSPPDDDDDEET 185

Query: 210 -IGADAHTDKSFFTILHQNGVNGLQIKTKNGDNWLGVQFSPTSFLVMAGDACLAWSNGRI 269
            +G  +HTDK+  TILHQ  V+GL++KTK+ D W+ V+ S  S LVM GD+  A  NGR+
Sbjct: 186 KLGLRSHTDKNIITILHQYQVDGLEVKTKD-DKWIKVKPSQDSVLVMVGDSLCALLNGRL 245

Query: 270 HSATHRVIIEGSKERYSTALFSYHKG--IIEIPHELVDEQHPLRFNPFNHYGLLGYFSTD 323
           HS  HRVI+ G K RYST LFS  K   II+ P ELVD++HP  F PF +   L +F T+
Sbjct: 246 HSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFFQTE 298

BLAST of Sgr007683 vs. ExPASy Swiss-Prot
Match: A0A0N9HQ36 (Deoxypodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=2-ODD PE=1 SV=1)

HSP 1 Score: 269.6 bits (688), Expect = 4.7e-71
Identity = 140/308 (45.45%), Postives = 186/308 (60.39%), Query Frame = 0

Query: 29  LQLPVINFSIENLGPESSNWFETCSHVRAALEEYGCFVAEYNLAPPELDDAIFKGLEELF 88
           L+LPVI+ S++NL P +++W    + VR ALEEYGCF A  +   PEL  A+     EL 
Sbjct: 7   LRLPVIDLSMKNLKPGTTSWNSVRTQVREALEEYGCFEAVIDAVSPELQKAVCNKGHELL 66

Query: 89  DLPTETKMKNVSDKPYHGFLDHRVPFVPPLHESCGIENAATFEAVESFVKLMWPSGNDRF 148
           +LP ETKM N +   Y GF       +P L+E  G+      E VE F  LMWP GN  F
Sbjct: 67  NLPLETKMLNGNKPEYDGFTS-----IPNLNEGMGVGRITDLEKVERFTNLMWPEGNKDF 126

Query: 149 CENVFSYSKMVSEMEQLVKRMVFESYGVEKYFDSHIASTTYLLRTMKYRIPQMGEENIGA 208
           CE V+SY K ++E++ ++K MVFES+G+EK+FDS   ST YLL  M+Y+ P     +   
Sbjct: 127 CETVYSYGKRMAEVDHILKMMVFESFGMEKHFDSFCESTNYLLHFMRYQQPGKDGRSPAL 186

Query: 209 DAHTDKSFFTILHQNGVNGLQIKTKNGDNWLGVQFSPT--SFLVMAGDACLAWSNGRIHS 268
             H DKS  TI++QN V GL+ +TK+G+ W+     PT  + +V+ GD  +AWSNGR+HS
Sbjct: 187 SLHKDKSILTIVNQNDVKGLEFETKDGE-WI----LPTADNHIVLLGDCFMAWSNGRLHS 246

Query: 269 ATHRVIIEGSKERYSTALFSYHKGIIEIPHELVDEQHPLRFNPFNHYGLLGYFSTDECDK 328
             HRV +  ++ R ST+ FS+ K IIE P ELVDE+HPL FNPF    LL Y  T E  K
Sbjct: 247 PLHRVTLVANQARLSTSSFSFPKDIIETPAELVDEEHPLLFNPFEITELLAYCFTKEGAK 304

Query: 329 NPSTAKAY 335
                K Y
Sbjct: 307 AVCDLKQY 304

BLAST of Sgr007683 vs. ExPASy Swiss-Prot
Match: Q945B5 (2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana OX=3702 GN=AOP2 PE=2 SV=1)

HSP 1 Score: 226.1 bits (575), Expect = 6.0e-58
Identity = 138/430 (32.09%), Postives = 198/430 (46.05%), Query Frame = 0

Query: 29  LQLPVINFSIENLGPESSNWFETCSHVRAALEEYGCFVAEYNLAPPELDDAIFKGLEELF 88
           LQLP+IN + + L P SS W E  S VR ALE++GCF A Y+    EL ++I K +EELF
Sbjct: 6   LQLPLINLADKTLEPGSSKWAEVRSDVRKALEDFGCFEASYDKVSLELQESIMKTMEELF 65

Query: 89  DLPTETKMKNVSDKPYHGFLDHRVPFVPPLHESCGIENAATFEAVESFVKLMWP--SGND 148
            LP ETK +NV  KPY G+L+H       L ES GI NA   E V  F + +WP   GN+
Sbjct: 66  ALPVETKQRNVCPKPYVGYLNHN-----NLSESLGISNANILENVNEFTQQLWPDGDGNE 125

Query: 149 RFCENVFSYSKMVSEMEQLVKRMVFESYGVEKYFDSHIASTTYLLRTMKYRIPQMGEEN- 208
              + +  +++ + E++ +V+RMV ES+G+EKY D H+ ST Y +R MKY  P  G+ N 
Sbjct: 126 NISKTIQLFAEKLVEIDVMVRRMVMESFGIEKYIDDHLKSTEYRMRLMKYIAPPEGDANT 185

Query: 209 ------------------------------------------------------------ 268
                                                                       
Sbjct: 186 TVDDYADLLAKLNIDGVEPNVGVKVNADISDDVNANPSVNAGVGANVNADTGVNDNLNVD 245

Query: 269 -------------------------------------------------------IGADA 328
                                                                  +G   
Sbjct: 246 AEANGDANIAVGGGVNANTDLGVGVNVNSNVAVNAKTGATSGDDVEANDDNEEKKLGLPC 305

Query: 329 HTDKSFFTILHQNGVNGLQIKTKNGDNWLGVQFSPTSFLVMAGDACLAWSNGRIHSATHR 338
           HTDK+ FT+L Q+ + GL++KTK+ + W+ V+ SP +F+V+AGD+  A  NGRI +  HR
Sbjct: 306 HTDKNLFTVLFQHEIEGLEVKTKD-EKWIRVKPSPNTFIVIAGDSLCALMNGRIRAPYHR 365

BLAST of Sgr007683 vs. ExPASy TrEMBL
Match: A0A6J1DH70 (2-oxoglutarate-dependent dioxygenase AOP3-like OS=Momordica charantia OX=3673 GN=LOC111021051 PE=3 SV=1)

HSP 1 Score: 551.2 bits (1419), Expect = 3.0e-153
Identity = 257/308 (83.44%), Postives = 283/308 (91.88%), Query Frame = 0

Query: 30  QLPVINFSIENLGPESSNWFETCSHVRAALEEYGCFVAEYNLAPPELDDAIFKGLEELFD 89
           +LPVIN SIEN+ PES+NW E CSH+RAALEEYGCF+ E+  A PELDD IFKGLEELFD
Sbjct: 11  KLPVINLSIENVSPESTNWLEICSHLRAALEEYGCFLGEFGSATPELDDEIFKGLEELFD 70

Query: 90  LPTETKMKNVSDKPYHGFLDHRVPFVPPLHESCGIENAATFEAVESFVKLMWPSGNDRFC 149
           LP ETKMKNVSDKPYHGFLDHR PFV PLH+S GIEN+ +FEAV+SFV LMWPSGN RFC
Sbjct: 71  LPLETKMKNVSDKPYHGFLDHRTPFVLPLHQSFGIENSESFEAVQSFVNLMWPSGNRRFC 130

Query: 150 ENVFSYSKMVSEMEQLVKRMVFESYGVEKYFDSHIASTTYLLRTMKYRIPQMGEENIGAD 209
           ENVFSY+KMVSEME+LVK MVFESYGV+KY+DSHIASTTYLLRTMKYRIP+MGEENIGAD
Sbjct: 131 ENVFSYTKMVSEMEKLVKVMVFESYGVKKYYDSHIASTTYLLRTMKYRIPEMGEENIGAD 190

Query: 210 AHTDKSFFTILHQNGVNGLQIKTKNGDNWLGVQFSPTSFLVMAGDACLAWSNGRIHSATH 269
           AHTDKSFFTILHQN VNGL+IKTK+G +WLGV+F+P SFLVMAGDACLAWSNGRIHSATH
Sbjct: 191 AHTDKSFFTILHQNEVNGLEIKTKDG-HWLGVEFTPNSFLVMAGDACLAWSNGRIHSATH 250

Query: 270 RVIIEGSKERYSTALFSYHKGIIEIPHELVDEQHPLRFNPFNHYGLLGYFSTDECDKNPS 329
           RV+IEG+KERYSTALFSYHKGIIEIP+ELVDE+HPLRF+PFNHYGLL YFST +  K PS
Sbjct: 251 RVMIEGTKERYSTALFSYHKGIIEIPYELVDEKHPLRFHPFNHYGLLAYFSTHDSHKMPS 310

Query: 330 TAKAYCGV 338
           TAKAYCGV
Sbjct: 311 TAKAYCGV 317

BLAST of Sgr007683 vs. ExPASy TrEMBL
Match: A0A6J1GA17 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662 GN=LOC111452291 PE=3 SV=1)

HSP 1 Score: 547.7 bits (1410), Expect = 3.3e-152
Identity = 254/311 (81.67%), Postives = 282/311 (90.68%), Query Frame = 0

Query: 30  QLPVINFSIENLGPESSNWFETCSHVRAALEEYGCFVAEYN---LAPPELDDAIFKGLEE 89
           ++PVINFSIENLGP+S+ W ETC+HVRAALEEYGCF+AEY        ELDDAIF GLE+
Sbjct: 10  EVPVINFSIENLGPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELDDAIFNGLEK 69

Query: 90  LFDLPTETKMKNVSDKPYHGFLDHRVPFVPPLHESCGIENAATFEAVESFVKLMWPSGND 149
           LFDLP +TKMKNVSDKPYHGFLDHR PFV PLHES GIENAA+FE VESFVKLMWPSGN 
Sbjct: 70  LFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVKLMWPSGNH 129

Query: 150 RFCENVFSYSKMVSEMEQLVKRMVFESYGVEKYFDSHIASTTYLLRTMKYRIPQMGEENI 209
            FC+++F+Y+KMVSEME+LVKRMVFESYGVEKY+DSHIASTTYLLR MKYRIP+MGE+NI
Sbjct: 130 HFCQDLFAYTKMVSEMEKLVKRMVFESYGVEKYYDSHIASTTYLLRGMKYRIPEMGEQNI 189

Query: 210 GADAHTDKSFFTILHQNGVNGLQIKTKNGDNWLGVQFSPTSFLVMAGDACLAWSNGRIHS 269
           GA+AHTDKSFFTILHQNGVNGL+IK K  D+W+G+QF+  SFLVMAGDACLAWSNGRIHS
Sbjct: 190 GAEAHTDKSFFTILHQNGVNGLEIKAKKDDHWIGLQFNSNSFLVMAGDACLAWSNGRIHS 249

Query: 270 ATHRVIIEGSKERYSTALFSYHKGIIEIPHELVDEQHPLRFNPFNHYGLLGYFSTDECDK 329
           ATHRVIIEGSKERYSTALFSYHKG+IEIP ELVDEQ PL+FNPFNHYGLLGYFS +EC+K
Sbjct: 250 ATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLGYFSKEECEK 309

Query: 330 NPSTAKAYCGV 338
            PSTA AYCGV
Sbjct: 310 TPSTAGAYCGV 320

BLAST of Sgr007683 vs. ExPASy TrEMBL
Match: A0A6J1K6A3 (2-oxoglutarate-dependent dioxygenase AOP3-like OS=Cucurbita maxima OX=3661 GN=LOC111492707 PE=3 SV=1)

HSP 1 Score: 546.6 bits (1407), Expect = 7.3e-152
Identity = 254/308 (82.47%), Postives = 280/308 (90.91%), Query Frame = 0

Query: 30  QLPVINFSIENLGPESSNWFETCSHVRAALEEYGCFVAEYNLAPPELDDAIFKGLEELFD 89
           ++P INFSIENL P+S+ W ETCSHVRAALEEYGCF+AE      ELDDAIF GLE+LFD
Sbjct: 10  EVPAINFSIENLRPKSTKWVETCSHVRAALEEYGCFLAECE-NNVELDDAIFNGLEKLFD 69

Query: 90  LPTETKMKNVSDKPYHGFLDHRVPFVPPLHESCGIENAATFEAVESFVKLMWPSGNDRFC 149
           LP ETKMKNVSDKPYHGFLDHR PFV PLHES GIENAA+FE VESFVKLMWPSGN  FC
Sbjct: 70  LPLETKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVKLMWPSGNHHFC 129

Query: 150 ENVFSYSKMVSEMEQLVKRMVFESYGVEKYFDSHIASTTYLLRTMKYRIPQMGEENIGAD 209
           E++F+Y+KMVSEME+LVKRMVFESYGVEKY+DSHIASTTYLLR MKYRIP+MGE+NIGA+
Sbjct: 130 EDLFAYTKMVSEMEKLVKRMVFESYGVEKYYDSHIASTTYLLRGMKYRIPEMGEQNIGAE 189

Query: 210 AHTDKSFFTILHQNGVNGLQIKTKNGDNWLGVQFSPTSFLVMAGDACLAWSNGRIHSATH 269
           AHTDKSFFTILHQNGVNGL+IK K  D+W+G+QF+P SFLVMAGDACLAWSNGRIHSATH
Sbjct: 190 AHTDKSFFTILHQNGVNGLEIKAKKDDHWIGLQFNPNSFLVMAGDACLAWSNGRIHSATH 249

Query: 270 RVIIEGSKERYSTALFSYHKGIIEIPHELVDEQHPLRFNPFNHYGLLGYFSTDECDKNPS 329
           RV+IEGSKERYSTALFSYHKG+IEIP ELVDEQ PL+FNPFNHYGLLGYFS +EC+K PS
Sbjct: 250 RVLIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLGYFSKEECEKTPS 309

Query: 330 TAKAYCGV 338
           TA AYCGV
Sbjct: 310 TAGAYCGV 316

BLAST of Sgr007683 vs. ExPASy TrEMBL
Match: A0A1S4DWR1 (2-oxoglutarate-dependent dioxygenase AOP3-like OS=Cucumis melo OX=3656 GN=LOC103490084 PE=3 SV=1)

HSP 1 Score: 439.9 bits (1130), Expect = 9.7e-120
Identity = 205/243 (84.36%), Postives = 222/243 (91.36%), Query Frame = 0

Query: 96  MKNVSDKPYHGFLDHRVPFVPPLHESCGIENAATFEAVESFVKLMWPSGNDRFCENVFSY 155
           MKNVSDKPYHGFLD R PFV PLH+S GIENA+++EAV+SFV L+WPSGN  FCEN+F+Y
Sbjct: 1   MKNVSDKPYHGFLDSRAPFVLPLHQSLGIENASSYEAVQSFVNLLWPSGNHHFCENLFAY 60

Query: 156 SKMVSEMEQLVKRMVFESYGVEKYFDSHIASTTYLLRTMKYRIPQMGEENIGADAHTDKS 215
           SK ++EMEQLVKRMVFESYGV KY DSHIASTTYLLRTMKYRIP+MGEENIGA+AHTDKS
Sbjct: 61  SKKITEMEQLVKRMVFESYGVGKYTDSHIASTTYLLRTMKYRIPKMGEENIGAEAHTDKS 120

Query: 216 FFTILHQ-NGVNGLQIKTKNGDNWLGVQFSPTSFLVMAGDACLAWSNGRIHSATHRVIIE 275
           FFTILHQ +GVNGLQIK KN D WLGV FSP  FLVMAGDACLAWSNGRIHSATHRVIIE
Sbjct: 121 FFTILHQFDGVNGLQIKPKNYDQWLGVHFSPNYFLVMAGDACLAWSNGRIHSATHRVIIE 180

Query: 276 GSKERYSTALFSYHKGIIEIPHELVDEQHPLRFNPFNHYGLLGYFSTDECDKNPSTAKAY 335
           G+KERYSTALFSYHKGIIEIP ELVD++HPLRF PFNHYGLLGYFSTD+CDK PSTA AY
Sbjct: 181 GNKERYSTALFSYHKGIIEIPQELVDDKHPLRFKPFNHYGLLGYFSTDDCDKMPSTANAY 240

Query: 336 CGV 338
           CGV
Sbjct: 241 CGV 243

BLAST of Sgr007683 vs. ExPASy TrEMBL
Match: A0A6P3YXF4 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Ziziphus jujuba OX=326968 GN=LOC107406140 PE=3 SV=1)

HSP 1 Score: 418.7 bits (1075), Expect = 2.3e-113
Identity = 196/313 (62.62%), Postives = 238/313 (76.04%), Query Frame = 0

Query: 28  LLQLPVINFSIENLGPESSNWFETCSHVRAALEEYGCFVAEYNLAPPELDDAIFKGLEEL 87
           L +LP I+FS ENL P +S W  T    R ALE+YGCFVA Y+   P+L D+IF+ L+EL
Sbjct: 6   LAKLPAIDFSKENLKPGTSCWSSTAIDARQALEQYGCFVAIYDKMSPQLHDSIFRELKEL 65

Query: 88  FDLPTETKMKNVSDKPYHGFL---DHRVPFVPPLHESCGIENAATFEAVESFVKLMWPSG 147
           FDLPTE KMKN+SDKPY G+        P VP LHES GIEN  T EAV+SF  LMWP+G
Sbjct: 66  FDLPTEIKMKNISDKPYFGYAAGNSATTPHVPSLHESLGIENTTTLEAVQSFTNLMWPAG 125

Query: 148 NDRFCENVFSYSKMVSEMEQLVKRMVFESYGVEKYFDSHIASTTYLLRTMKYRIPQMGEE 207
           N  FCE V SY+K+V+E+EQ+VKRMVFESYG EKY+DSH+ STTYLLR +KYR P++ E 
Sbjct: 126 NHSFCETVLSYTKLVAELEQMVKRMVFESYGAEKYYDSHVGSTTYLLRQIKYRAPEIDES 185

Query: 208 NIGADAHTDKSFFTILHQNGVNGLQIKTKNGDNWLGVQFSPTSFLVMAGDACLAWSNGRI 267
           ++G + HTDKSF TILHQN V G ++KTK+G  W+G + SP+SFLV+AGDA LAWSN RI
Sbjct: 186 DVGCEIHTDKSFITILHQNEVEGFELKTKDG-YWIGFEPSPSSFLVLAGDAFLAWSNDRI 245

Query: 268 HSATHRVIIEGSKERYSTALFSYHKGIIEIPHELVDEQHPLRFNPFNHYGLLGYFSTDEC 327
           HSA HRV+++GS+ER+S  +FSYH GIIEIP E+VDEQHPL+F  F HYGLL YF T   
Sbjct: 246 HSAAHRVVMKGSRERFSVGVFSYHNGIIEIPVEVVDEQHPLKFKAFEHYGLLNYFYTAPG 305

Query: 328 DKNPSTAKAYCGV 338
            K  STAKAYCG+
Sbjct: 306 PKTDSTAKAYCGL 317

BLAST of Sgr007683 vs. TAIR 10
Match: AT1G52800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 323.2 bits (827), Expect = 2.5e-88
Identity = 147/309 (47.57%), Postives = 210/309 (67.96%), Query Frame = 0

Query: 30  QLPVINF-SIENLGPESSNWFETCSHVRAALEEYGCFVAEYNLAPPELDDAIFKGLEELF 89
           ++P+++  S + L P +S W         ALEEYGCF+A Y+    +LDD+IF   EELF
Sbjct: 9   KVPILDLTSQQELKPNTSTWRSVSREACEALEEYGCFLAVYDGVTQQLDDSIFAAAEELF 68

Query: 90  DLPTETKMKNVSDKPYHGFLDHRVPFVPPLHESCGIENAATFEAVESFVKLMWPSGNDRF 149
           DLPTETK KNV++KPYHG++  ++P + PLHE  G++     E  + F  LMWP GNDRF
Sbjct: 69  DLPTETKKKNVNEKPYHGYVG-QMPVI-PLHEGLGVDYVTNKEIAQRFTHLMWPQGNDRF 128

Query: 150 CENVFSYSKMVSEMEQLVKRMVFESYGVEKYFDSHIASTTYLLRTMKYRIPQMGEENIGA 209
           C  V ++S  V+E+++LV RM+FE+YGVEK+++SH+ S TYLL+ +KY  P         
Sbjct: 129 CNTVHTFSNAVAELDRLVVRMIFENYGVEKHYESHVGSKTYLLKFLKYLAPPESISMPAF 188

Query: 210 DAHTDKSFFTILHQNGVNGLQIKTKNGDNWLGVQFSPTSFLVMAGDACLAWSNGRIHSAT 269
             HTDK+F +ILHQN VNGL++K+K+G+ W+ +Q  P S++VMAGD  + WSN RI S  
Sbjct: 189 PQHTDKTFLSILHQNDVNGLEVKSKDGE-WISLQLPPKSYVVMAGDISMGWSNDRIRSCE 248

Query: 270 HRVIIEGSKERYSTALFSYHKGIIEIPHELVDEQHPLRFNPFNHYGLLGYFSTDECDKNP 329
           HRV +EG K RY+  LFS+   ++ IP ELVD++HPL + PF++  L+ +++T E  +  
Sbjct: 249 HRVTMEGDKTRYTLGLFSFLTDLVSIPEELVDDKHPLMYKPFDNIALINFYTTKEGREAN 308

Query: 330 STAKAYCGV 338
           ST KAYCG+
Sbjct: 309 STLKAYCGI 314

BLAST of Sgr007683 vs. TAIR 10
Match: AT1G52790.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 318.9 bits (816), Expect = 4.8e-87
Identity = 147/308 (47.73%), Postives = 212/308 (68.83%), Query Frame = 0

Query: 30  QLPVINFSIENLGPESSNWFETCSHVRAALEEYGCFVAEY-NLAPPELDDAIFKGLEELF 89
           ++P ++FS E+L P +  W  T  ++R ALEEYGCF+ +  +  P +L D +F  L +LF
Sbjct: 4   KIPTLDFSREDLKPGTKYWESTRENIRQALEEYGCFIIDLKDKTPLDLLDRVFGSLVDLF 63

Query: 90  DLPTETKMKNVSDKPYHGFLDHRVPFVPPLHESCGIENAATFEAVESFVKLMWPSGNDRF 149
           DLPT+TKMKN  DKP +G++  ++P + PLHES GI+NA + EA  SF  LMWP GN+ F
Sbjct: 64  DLPTQTKMKNKYDKPLNGYVG-QIPAL-PLHESLGIDNATSLEATRSFTGLMWPQGNEHF 123

Query: 150 CENVFSYSKMVSEMEQLVKRMVFESYGVEKYFDSHIASTTYLLRTMKYRIPQMGEENIGA 209
            E ++ Y++  +E++Q+V RMVF+SY VEKY+D +I STTYLLR +K R P      +G 
Sbjct: 124 SECLYKYAEFAAELDQMVTRMVFQSYNVEKYYDPYIESTTYLLRVLKNRAPNNENPTLGF 183

Query: 210 DAHTDKSFFTILHQNGVNGLQIKTKNGDNWLGVQFSPTSFLVMAGDACLAWSNGRIHSAT 269
             HTDKSF TILHQ+ VNGL+++T+ G+       SP+ F+V+AGDA +AWSN R+ S  
Sbjct: 184 VTHTDKSFTTILHQDQVNGLEMETREGERININLSSPSLFMVVAGDALMAWSNDRVWSPR 243

Query: 270 HRVIIEGSKERYSTALFSYHKGIIEIPHELVDEQHPLRFNPFNHYGLLGYFSTDECDKNP 329
           H+V++ G  +RYS  +F+++ G +++P EL+D QHPL + PF+H GLL ++ TD   K+ 
Sbjct: 244 HQVLVSGETDRYSLGMFAFNNGTLQVPEELIDHQHPLMYKPFDHLGLLRFYRTDTGYKSE 303

Query: 330 STAKAYCG 337
              KAYCG
Sbjct: 304 CPIKAYCG 309

BLAST of Sgr007683 vs. TAIR 10
Match: AT1G52820.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 297.0 bits (759), Expect = 2.0e-80
Identity = 147/318 (46.23%), Postives = 212/318 (66.67%), Query Frame = 0

Query: 23  SVTPQL-LQLPVINFSIENLGPESSNWFETCSHVRAALEEYGCFVAEYNLAPPELDDAIF 82
           S TP L L+LPVI+FS +NL P    W  T + V+ AL++YG F A ++  P EL  ++F
Sbjct: 3   SETPLLPLRLPVIDFSNKNLKPGEPEWDLTRADVQKALQDYGYFEASFDRIPFELRKSVF 62

Query: 83  KGLEELFDLPTETKMKNVSDKPYHGFLDHRVPFVPPLHESCGIENAATFEAVESFVKLMW 142
             LEELFDLP +TK++NVS KP+HG++  + P V PL+ES GI+++   E V++F + +W
Sbjct: 63  GALEELFDLPLQTKLRNVSKKPFHGYVG-QYPMV-PLYESMGIDDSDIAEKVDAFTEKLW 122

Query: 143 PSGNDRFCENVFSYSKMVSEMEQLVKRMVFESYGVEKYFDSHIASTTYLLRTMKYRIPQM 202
           P GN  F   + S+SK +SE++  ++RM+ ES+G++KY D H+ ST YLLR MKY+ P  
Sbjct: 123 PQGNISFSTTIQSFSKKLSELDITIRRMIMESFGLDKYIDEHLHSTNYLLRVMKYKGPDT 182

Query: 203 GEENIGADAHTDKSFFTILHQNGVNGLQIKTKNGDNWLGVQFSPTSFLVMAGDACLAWSN 262
            E  +G +AHTDK+  TIL+QN V GL+++TK+  NW+ V+ +  SF VM GD+  A  N
Sbjct: 183 EETKVGLNAHTDKNIVTILYQNHVEGLEVQTKD-KNWIKVKPTQDSFTVMIGDSLYALLN 242

Query: 263 GRIHSATHRVIIEGSKERYSTALFSYHKG--IIEIPHELVDEQHPLRFNPFNHYGLLGYF 322
           GR+HS  HRV++ G++ RYS  LFS  K   I+  P ELVDE+HP  F PF+H   L ++
Sbjct: 243 GRLHSPYHRVMMTGTETRYSLGLFSIPKAGHIVSSPDELVDEEHPRLFKPFDHVEFLQFY 302

Query: 323 STDECDKNPSTAKAYCGV 338
            T+   ++ S  K YCG+
Sbjct: 303 YTEAGQRSQSALKTYCGI 317

BLAST of Sgr007683 vs. TAIR 10
Match: AT1G15540.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 289.3 bits (739), Expect = 4.1e-78
Identity = 145/313 (46.33%), Postives = 195/313 (62.30%), Query Frame = 0

Query: 30  QLPVINFSIENLGPESSNWFETCSHVRAALEEYGCFVAEYNLAPPELDDAIFKGLEELFD 89
           +LP+IN S +NL   +  W  T   VR A+E +G FVAEYN  P  L  +I +  +EL D
Sbjct: 8   ELPIINLSDKNLKQNTELWSFTRDSVREAMEHHGWFVAEYNNFPTGLHQSILEAAKELLD 67

Query: 90  LPTETKMKNVSDKPYHGFLDHRVPFVPPLHESCGIENAATFEAVESFVKLMWP---SGND 149
           LP E K+KN + K  HG++   +    P+HE  GI+     +    F +LMWP     ND
Sbjct: 68  LPVEIKLKNENHKAGHGYIT-MMSDGQPVHEGLGIDQVNDVQQCRGFSRLMWPDDHDDND 127

Query: 150 RFCENVFSYSKMVSEMEQLVKRMVFESYGVEKYFDSHIASTTYLLRTMKYRIPQMGEENI 209
           RFCE V +Y+KM +E+EQLV RM+FESY VEKY + +I  T YLLR +KYR    GE N 
Sbjct: 128 RFCETVHAYAKMQAELEQLVIRMLFESYNVEKYTEKYIGGTRYLLRLLKYRRLPNGEPNR 187

Query: 210 GADAHTDKSFFTILHQNGVNGLQIKTKNGDNWLGVQFSPTSFLVMAGDACLAWSNGRIHS 269
              +HTDKSF +ILHQN + GL +K++  D W     SPT F+V+AGDA +AWSN RI +
Sbjct: 188 KFISHTDKSFISILHQNHITGLMLKSEKEDVWYHFTPSPTRFVVIAGDAIMAWSNDRIKA 247

Query: 270 ATHRVIIEGSKERYSTALFSYHKGIIEIPHELVDEQHPLRFNPFNHYGLLGYFSTDE--C 329
             H+V +E  + RYS   FS+ +G+I  P E+VD+ HPL +NPF+H GLL Y+ T E   
Sbjct: 248 CYHKVEMESVEMRYSLGFFSFQEGMISTPEEMVDKDHPLAYNPFHHDGLLEYYETLEVHL 307

Query: 330 DKNPSTAKAYCGV 338
             + +  KAYCG+
Sbjct: 308 KAHRTMTKAYCGI 319

BLAST of Sgr007683 vs. TAIR 10
Match: AT1G80320.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 277.7 bits (709), Expect = 1.2e-74
Identity = 139/317 (43.85%), Postives = 197/317 (62.15%), Query Frame = 0

Query: 31  LPVINFSIENLGPESSNWFETCSHVRAALEEYGCFVAEYNLAPPELDDAIFKGLEELFDL 90
           LP+++FS + L   +S+W  T   VR A+E  G FVAE++    +L D +  G++E++ L
Sbjct: 9   LPILDFSSDKLVRGTSHWITTRDDVRRAMEGQGWFVAEFSGVSSDLRDNLLAGMKEMYYL 68

Query: 91  PTETKMKNVSDKPYHGFL-----DHRVPFVPPLHESCGIENAATFEAVESFVKLMWPSGN 150
           P + K+KN + K  HG++     D+R+      HES GI+ A   +A + F KL+WP GN
Sbjct: 69  PDQIKIKNENHKASHGYMSMVVDDYRI------HESLGIDYATELQACKDFSKLLWPQGN 128

Query: 151 DRFCENVFSYSKMVSEMEQLVKRMVFESYGVE--KYFDSHIASTTYLLRTMKYRIPQMGE 210
           D FC+    Y+  ++E++Q V RM++ESYG++  K+  SH  ST YLLR + YR  Q GE
Sbjct: 129 DPFCQTTHMYAMTMAELDQTVMRMLYESYGMDEKKHSVSHSESTRYLLRMLSYRRQQNGE 188

Query: 211 ENIGADAHTDKSFFTILHQNGVNGLQIKTKNGDNWLGVQFSPTSFLVMAGDACLAWSNGR 270
            N G  +HTDKSF +ILHQN V GLQ+KT  G  W+G   SPT F+V++G    AWSN R
Sbjct: 189 ANTGFVSHTDKSFMSILHQNHVGGLQLKTMTG-QWVGFNPSPTRFVVLSGMGLTAWSNDR 248

Query: 271 IHSATHRVIIEGSKERYSTALFSYHKGIIEIPHELVDEQHPLRFNPFNHYGLLGYFSTDE 330
           I +  H+V++   + RYS   FS+HKG I  P ELVD+QHPLR+NPF H GLL ++ +  
Sbjct: 249 IKACYHKVVMSADEIRYSLGFFSFHKGTIRTPEELVDDQHPLRYNPFEHDGLLRFYESYI 308

Query: 331 CDKNPST---AKAYCGV 338
                S+    + YCGV
Sbjct: 309 NSLKKSSEDLLQIYCGV 318

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022153580.16.2e-15383.442-oxoglutarate-dependent dioxygenase AOP3-like [Momordica charantia][more]
XP_038878200.18.0e-15383.332-oxoglutarate-dependent dioxygenase AOP3-like [Benincasa hispida][more]
XP_023525673.12.3e-15280.882-oxoglutarate-dependent dioxygenase AOP3-like [Cucurbita pepo subsp. pepo][more]
XP_022948698.16.8e-15281.67probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita moschata][more]
XP_022997882.11.5e-15182.472-oxoglutarate-dependent dioxygenase AOP3-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9ZTA32.5e-7245.51Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=37... [more]
Q945B64.2e-7245.66Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=... [more]
Q944Z52.1e-7146.00Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis tha... [more]
A0A0N9HQ364.7e-7145.45Deoxypodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=2-ODD PE=... [more]
Q945B56.0e-5832.092-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana OX=3702 GN=AOP... [more]
Match NameE-valueIdentityDescription
A0A6J1DH703.0e-15383.442-oxoglutarate-dependent dioxygenase AOP3-like OS=Momordica charantia OX=3673 GN... [more]
A0A6J1GA173.3e-15281.67probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662... [more]
A0A6J1K6A37.3e-15282.472-oxoglutarate-dependent dioxygenase AOP3-like OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A1S4DWR19.7e-12084.362-oxoglutarate-dependent dioxygenase AOP3-like OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A6P3YXF42.3e-11362.62probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Ziziphus jujuba OX=326968 ... [more]
Match NameE-valueIdentityDescription
AT1G52800.12.5e-8847.572-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G52790.14.8e-8747.732-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G52820.12.0e-8046.232-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G15540.14.1e-7846.332-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G80320.11.2e-7443.852-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 4..316
e-value: 4.4E-55
score: 189.5
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 203..285
e-value: 1.1E-17
score: 64.3
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 31..107
e-value: 2.0E-9
score: 38.2
NoneNo IPR availablePANTHERPTHR47990:SF172-OXOGLUTARATE-DEPENDENT DIOXYGENASE AOP3-LIKEcoord: 28..336
NoneNo IPR availablePANTHERPTHR479902-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATEDcoord: 28..336
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 22..319
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 186..288
score: 9.731292

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr007683.1Sgr007683.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0051213 dioxygenase activity
molecular_function GO:0046872 metal ion binding