Sgr005287 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr005287 vs. NCBI nr
Match: XP_022138649.1 (1-aminocyclopropane-1-carboxylate oxidase homolog 1-like [Momordica charantia])

HSP 1 Score: 622.9 bits (1605), Expect = 1.8e-174
Identity = 301/354 (85.03%), Postives = 319/354 (90.11%), Query Frame = 0

Query: 5   GTESFNSRRTELESFDNSKAGVKGLLDAGITKIPRIFHCDKQDVNASAESELSIPIIDLQ 64
           GTES +SRR ELESFDNSKAG KGL DAGITKIP IFHC KQD+NA+AESELSIPIIDL 
Sbjct: 7   GTESLSSRRAELESFDNSKAGAKGLSDAGITKIPPIFHCQKQDLNAAAESELSIPIIDLH 66

Query: 65  GGGEQVVDQIGSACRKWGFFQVVNHGIPVEVLDKMIDGIRGFHEQDLELKKQFYSRDRSK 124
           GG E V+DQIGSACRKWGFFQVVNHGIPVEVLD MIDGIRGFHEQDLE KKQFYSRDR K
Sbjct: 67  GGEEVVMDQIGSACRKWGFFQVVNHGIPVEVLDGMIDGIRGFHEQDLEWKKQFYSRDRGK 126

Query: 125 KVTHFSNFDLFQTKAANWRDTLSLSMAPNHPLPEEIPEICREIVMEYTKEVKKLGLTLLE 184
           KV +FSNFDLFQ+KAANW+DTLSLSMAP HP P EIPEICREIV+EYTKEVKKLG  LLE
Sbjct: 127 KVAYFSNFDLFQSKAANWKDTLSLSMAPIHPDPHEIPEICREIVIEYTKEVKKLGWILLE 186

Query: 185 LISKALGLQARHLKELGCADGLLMFGHYYPACPEPELTVGTSNHTDVDFITILLQDQIGG 244
           LISKALGL+  HLK++GCADG+LM  HYYP CPEP LT+GT+NHTDV FITILLQDQIGG
Sbjct: 187 LISKALGLEGSHLKDIGCADGVLMLSHYYPPCPEPHLTIGTANHTDVAFITILLQDQIGG 246

Query: 245 LQVLHQNRWFDVPPLHGALVINLGDFLQIISNDRMVSVSHRVVAKKLGPRISVASLFRPY 304
           LQVLH NRW  VPPLHGALVINLGDFLQI+SND MVSVSHRV AKK+GPR+SVAS FRPY
Sbjct: 247 LQVLHHNRWLHVPPLHGALVINLGDFLQIVSNDAMVSVSHRVAAKKIGPRVSVASQFRPY 306

Query: 305 IGEDVGNSNVYEPIKELLSEDNPPIYKQVDPKEYIKLHYSKGLDGSSKLELFKL 359
           +GEDVGNS VYEPIKELLSEDNPP YKQVDPKEYIKLH SKG DGSSKLELFKL
Sbjct: 307 VGEDVGNSKVYEPIKELLSEDNPPKYKQVDPKEYIKLHASKGFDGSSKLELFKL 360

BLAST of Sgr005287 vs. NCBI nr
Match: XP_022930401.1 (1-aminocyclopropane-1-carboxylate oxidase homolog 1-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 581.3 bits (1497), Expect = 5.9e-162
Identity = 279/360 (77.50%), Postives = 322/360 (89.44%), Query Frame = 0

Query: 1   MAANGTESFNSRRTELESFDNSKAGVKGLLDAGITKIPRIFHC--DKQDVNASAESELSI 60
           MAA  TES NSRR++LE+F+N+K+GVKGLLDAGITKIP IFHC        A+ ESE SI
Sbjct: 52  MAAIKTESLNSRRSQLEAFENTKSGVKGLLDAGITKIPSIFHCHVKPHTKTAATESESSI 111

Query: 61  PIIDLQGGGEQVVDQIGSACRKWGFFQVVNHGIPVEVLDKMIDGIRGFHEQDLELKKQFY 120
           PIIDL G  E+VVD+I +ACRKWGFFQVVNHGIPVEVL+++I+G+RGFHEQD ELKKQFY
Sbjct: 112 PIIDLHGSVEEVVDEIRNACRKWGFFQVVNHGIPVEVLEQVINGVRGFHEQDPELKKQFY 171

Query: 121 SRDRSKKVTHFSNFDLFQTKAANWRDTLSLSMAPNHPLPEEIPEICREIVMEYTKEVKKL 180
           SRDR+KKV++FSNFDLFQ+KAANWRDTLS SMAP+ PLPEEIP+ICREIVMEYT++VKKL
Sbjct: 172 SRDRTKKVSYFSNFDLFQSKAANWRDTLSFSMAPSLPLPEEIPQICREIVMEYTEQVKKL 231

Query: 181 GLTLLELISKALGLQARHLKELGCADGLLMFGHYYPACPEPELTVGTSNHTDVDFITILL 240
           GLTL ELISKAL L   HLK++GCA+GLLM GHYYPACPEP+LT+GT+NHTDV FITILL
Sbjct: 232 GLTLFELISKALELGDTHLKDMGCAEGLLMLGHYYPACPEPDLTLGTTNHTDVAFITILL 291

Query: 241 QDQIGGLQVLHQNRWFDVPPLHGALVINLGDFLQIISNDRMVSVSHRVVAKKLGPRISVA 300
           QDQIGGLQV H N+WFDVPPLHGALVIN GDFLQIISND+MVSVSHRVVAK++GPR S+A
Sbjct: 292 QDQIGGLQVFHDNQWFDVPPLHGALVINAGDFLQIISNDKMVSVSHRVVAKEIGPRTSIA 351

Query: 301 SLFRPYIGEDVGNSNVYEPIKELLSEDNPPIYKQVDPKEYIKLHYSKGLDGSSKLELFKL 359
           S FRPY+G+DV NS +YEPIKELLSEDNPPIY+QV+PKE+I+LH+S GLD  SKL+LFKL
Sbjct: 352 SQFRPYMGKDVKNSKLYEPIKELLSEDNPPIYRQVNPKEFIELHFSNGLDECSKLDLFKL 411

BLAST of Sgr005287 vs. NCBI nr
Match: XP_022930403.1 (1-aminocyclopropane-1-carboxylate oxidase homolog 1-like isoform X4 [Cucurbita moschata])

HSP 1 Score: 576.6 bits (1485), Expect = 1.5e-160
Identity = 279/361 (77.29%), Postives = 322/361 (89.20%), Query Frame = 0

Query: 1   MAANGTESFNSRRTELESFDNSKAGVKGLLDAGITKIPRIFHC--DKQDVNASAESELSI 60
           MAA  TES NSRR++LE+F+N+K+GVKGLLDAGITKIP IFHC        A+ ESE SI
Sbjct: 1   MAAIKTESLNSRRSQLEAFENTKSGVKGLLDAGITKIPSIFHCHVKPHTKTAATESESSI 60

Query: 61  PIIDLQGGGEQVVDQIGSACRKWGFFQVVNHGIPVEVLDKMIDGIRGFHEQDLELKKQFY 120
           PIIDL G  E+VVD+I +ACRKWGFFQVVNHGIPVEVL+++I+G+RGFHEQD ELKKQFY
Sbjct: 61  PIIDLHGSVEEVVDEIRNACRKWGFFQVVNHGIPVEVLEQVINGVRGFHEQDPELKKQFY 120

Query: 121 SRDRSKKVTHFSNFDLFQTKAANWRDTLSLSMAPNHPLPEEIPEICREIVMEYTKEVKKL 180
           SRDR+KKV++FSNFDLFQ+KAANWRDTLS SMAP+ PLPEEIP+ICREIVMEYT++VKKL
Sbjct: 121 SRDRTKKVSYFSNFDLFQSKAANWRDTLSFSMAPSLPLPEEIPQICREIVMEYTEQVKKL 180

Query: 181 GLTLLELISKALGLQARHLKELGCADGLLMFGHYYPACPEPELTVGTSNHTDVDFITILL 240
           GLTL ELISKAL L   HLK++GCA+GLLM GHYYPACPEP+LT+GT+NHTDV FITILL
Sbjct: 181 GLTLFELISKALELGDTHLKDMGCAEGLLMLGHYYPACPEPDLTLGTTNHTDVAFITILL 240

Query: 241 QDQIGGLQVLHQNRWFDVPPLHGALVINLGDFL-QIISNDRMVSVSHRVVAKKLGPRISV 300
           QDQIGGLQV H N+WFDVPPLHGALVIN GDFL QIISND+MVSVSHRVVAK++GPR S+
Sbjct: 241 QDQIGGLQVFHDNQWFDVPPLHGALVINAGDFLQQIISNDKMVSVSHRVVAKEIGPRTSI 300

Query: 301 ASLFRPYIGEDVGNSNVYEPIKELLSEDNPPIYKQVDPKEYIKLHYSKGLDGSSKLELFK 359
           AS FRPY+G+DV NS +YEPIKELLSEDNPPIY+QV+PKE+I+LH+S GLD  SKL+LFK
Sbjct: 301 ASQFRPYMGKDVKNSKLYEPIKELLSEDNPPIYRQVNPKEFIELHFSNGLDECSKLDLFK 360

BLAST of Sgr005287 vs. NCBI nr
Match: XP_022930400.1 (1-aminocyclopropane-1-carboxylate oxidase homolog 1-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 576.6 bits (1485), Expect = 1.5e-160
Identity = 279/361 (77.29%), Postives = 322/361 (89.20%), Query Frame = 0

Query: 1   MAANGTESFNSRRTELESFDNSKAGVKGLLDAGITKIPRIFHC--DKQDVNASAESELSI 60
           MAA  TES NSRR++LE+F+N+K+GVKGLLDAGITKIP IFHC        A+ ESE SI
Sbjct: 52  MAAIKTESLNSRRSQLEAFENTKSGVKGLLDAGITKIPSIFHCHVKPHTKTAATESESSI 111

Query: 61  PIIDLQGGGEQVVDQIGSACRKWGFFQVVNHGIPVEVLDKMIDGIRGFHEQDLELKKQFY 120
           PIIDL G  E+VVD+I +ACRKWGFFQVVNHGIPVEVL+++I+G+RGFHEQD ELKKQFY
Sbjct: 112 PIIDLHGSVEEVVDEIRNACRKWGFFQVVNHGIPVEVLEQVINGVRGFHEQDPELKKQFY 171

Query: 121 SRDRSKKVTHFSNFDLFQTKAANWRDTLSLSMAPNHPLPEEIPEICREIVMEYTKEVKKL 180
           SRDR+KKV++FSNFDLFQ+KAANWRDTLS SMAP+ PLPEEIP+ICREIVMEYT++VKKL
Sbjct: 172 SRDRTKKVSYFSNFDLFQSKAANWRDTLSFSMAPSLPLPEEIPQICREIVMEYTEQVKKL 231

Query: 181 GLTLLELISKALGLQARHLKELGCADGLLMFGHYYPACPEPELTVGTSNHTDVDFITILL 240
           GLTL ELISKAL L   HLK++GCA+GLLM GHYYPACPEP+LT+GT+NHTDV FITILL
Sbjct: 232 GLTLFELISKALELGDTHLKDMGCAEGLLMLGHYYPACPEPDLTLGTTNHTDVAFITILL 291

Query: 241 QDQIGGLQVLHQNRWFDVPPLHGALVINLGDFL-QIISNDRMVSVSHRVVAKKLGPRISV 300
           QDQIGGLQV H N+WFDVPPLHGALVIN GDFL QIISND+MVSVSHRVVAK++GPR S+
Sbjct: 292 QDQIGGLQVFHDNQWFDVPPLHGALVINAGDFLQQIISNDKMVSVSHRVVAKEIGPRTSI 351

Query: 301 ASLFRPYIGEDVGNSNVYEPIKELLSEDNPPIYKQVDPKEYIKLHYSKGLDGSSKLELFK 359
           AS FRPY+G+DV NS +YEPIKELLSEDNPPIY+QV+PKE+I+LH+S GLD  SKL+LFK
Sbjct: 352 ASQFRPYMGKDVKNSKLYEPIKELLSEDNPPIYRQVNPKEFIELHFSNGLDECSKLDLFK 411

BLAST of Sgr005287 vs. NCBI nr
Match: XP_022930402.1 (1-aminocyclopropane-1-carboxylate oxidase homolog 1-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 576.6 bits (1485), Expect = 1.5e-160
Identity = 279/361 (77.29%), Postives = 322/361 (89.20%), Query Frame = 0

Query: 1   MAANGTESFNSRRTELESFDNSKAGVKGLLDAGITKIPRIFHC--DKQDVNASAESELSI 60
           MAA  TES NSRR++LE+F+N+K+GVKGLLDAGITKIP IFHC        A+ ESE SI
Sbjct: 21  MAAIKTESLNSRRSQLEAFENTKSGVKGLLDAGITKIPSIFHCHVKPHTKTAATESESSI 80

Query: 61  PIIDLQGGGEQVVDQIGSACRKWGFFQVVNHGIPVEVLDKMIDGIRGFHEQDLELKKQFY 120
           PIIDL G  E+VVD+I +ACRKWGFFQVVNHGIPVEVL+++I+G+RGFHEQD ELKKQFY
Sbjct: 81  PIIDLHGSVEEVVDEIRNACRKWGFFQVVNHGIPVEVLEQVINGVRGFHEQDPELKKQFY 140

Query: 121 SRDRSKKVTHFSNFDLFQTKAANWRDTLSLSMAPNHPLPEEIPEICREIVMEYTKEVKKL 180
           SRDR+KKV++FSNFDLFQ+KAANWRDTLS SMAP+ PLPEEIP+ICREIVMEYT++VKKL
Sbjct: 141 SRDRTKKVSYFSNFDLFQSKAANWRDTLSFSMAPSLPLPEEIPQICREIVMEYTEQVKKL 200

Query: 181 GLTLLELISKALGLQARHLKELGCADGLLMFGHYYPACPEPELTVGTSNHTDVDFITILL 240
           GLTL ELISKAL L   HLK++GCA+GLLM GHYYPACPEP+LT+GT+NHTDV FITILL
Sbjct: 201 GLTLFELISKALELGDTHLKDMGCAEGLLMLGHYYPACPEPDLTLGTTNHTDVAFITILL 260

Query: 241 QDQIGGLQVLHQNRWFDVPPLHGALVINLGDFL-QIISNDRMVSVSHRVVAKKLGPRISV 300
           QDQIGGLQV H N+WFDVPPLHGALVIN GDFL QIISND+MVSVSHRVVAK++GPR S+
Sbjct: 261 QDQIGGLQVFHDNQWFDVPPLHGALVINAGDFLQQIISNDKMVSVSHRVVAKEIGPRTSI 320

Query: 301 ASLFRPYIGEDVGNSNVYEPIKELLSEDNPPIYKQVDPKEYIKLHYSKGLDGSSKLELFK 359
           AS FRPY+G+DV NS +YEPIKELLSEDNPPIY+QV+PKE+I+LH+S GLD  SKL+LFK
Sbjct: 321 ASQFRPYMGKDVKNSKLYEPIKELLSEDNPPIYRQVNPKEFIELHFSNGLDECSKLDLFK 380

BLAST of Sgr005287 vs. ExPASy Swiss-Prot
Match: Q84MB3 (1-aminocyclopropane-1-carboxylate oxidase homolog 1 OS=Arabidopsis thaliana OX=3702 GN=At1g06620 PE=2 SV=1)

HSP 1 Score: 412.9 bits (1060), Expect = 3.6e-114
Identity = 198/356 (55.62%), Postives = 255/356 (71.63%), Query Frame = 0

Query: 12  RRTELESFDNSKAGVKGLLDAGITKIPRIFHCDKQDVNA---SAESELSIPIIDLQGGG- 71
           R T L++FD +K GVKGL+DAGIT+IP IF      + +    + S+ SIP IDL+GGG 
Sbjct: 13  RSTLLKAFDETKTGVKGLIDAGITEIPSIFRAPPATLTSPKPPSSSDFSIPTIDLKGGGT 72

Query: 72  -----EQVVDQIGSACRKWGFFQVVNHGIPVEVLDKMIDGIRGFHEQDLELKKQFYSRDR 131
                  +V++IG A  KWGFFQV+NHGIP++VL+KMIDGIR FHEQD E+KK FYSRD 
Sbjct: 73  DSITRRSLVEKIGDAAEKWGFFQVINHGIPMDVLEKMIDGIREFHEQDTEVKKGFYSRDP 132

Query: 132 SKKVTHFSNFDLFQTKAANWRDTLSLSMAPNHPLPEEIPEICREIVMEYTKEVKKLGLTL 191
           + K+ + SNFDLF + AANWRDTL    AP+ P PE++P  C E+++EY+KEV KLG  L
Sbjct: 133 ASKMVYSSNFDLFSSPAANWRDTLGCYTAPDPPRPEDLPATCGEMMIEYSKEVMKLGKLL 192

Query: 192 LELISKALGLQARHLKELGCADGLLMFGHYYPACPEPELTVGTSNHTDVDFITILLQDQI 251
            EL+S+ALGL   HLK++ C + LL+ GHYYP CP+P+LT+G + H+D  F+TILLQD I
Sbjct: 193 FELLSEALGLNTNHLKDMDCTNSLLLLGHYYPPCPQPDLTLGLTKHSDNSFLTILLQDHI 252

Query: 252 GGLQVLHQNRWFDVPPLHGALVINLGDFLQIISNDRMVSVSHRVVAKKLGPRISVASLFR 311
           GGLQVLH   W DVPP+ GALV+N+GD LQ+I+ND+ +SV HRV+A   GPRISVA  F 
Sbjct: 253 GGLQVLHDQYWVDVPPVPGALVVNVGDLLQLITNDKFISVEHRVLANVAGPRISVACFFS 312

Query: 312 PYIGEDVGNSNVYEPIKELLSEDNPPIYKQVDPKEYIKLHYSKGLDGSSKLELFKL 359
            Y+   + N  VY PIKE+LSE+NPP Y+     EY K + SKG DG+S L   K+
Sbjct: 313 SYL---MANPRVYGPIKEILSEENPPNYRDTTITEYAKFYRSKGFDGTSGLLYLKI 365

BLAST of Sgr005287 vs. ExPASy Swiss-Prot
Match: O04847 (Deacetoxyvindoline 4-hydroxylase OS=Catharanthus roseus OX=4058 GN=D4H PE=1 SV=2)

HSP 1 Score: 390.2 bits (1001), Expect = 2.5e-107
Identity = 188/356 (52.81%), Postives = 260/356 (73.03%), Query Frame = 0

Query: 15  ELESFDNSKAGVKGLLDAGITKIPRIFHCDKQDVN----ASAESELSIPIIDLQGGG--- 74
           EL++FD +KAGVKG++D GITKIPRIF    ++++       +S++ IP+I+L G     
Sbjct: 44  ELKAFDETKAGVKGIVDTGITKIPRIFIDQPKNLDRISVCRGKSDIKIPVINLNGLSSNS 103

Query: 75  ---EQVVDQIGSACRKWGFFQVVNHGIPVEVLDKMIDGIRGFHEQDLELKKQFYSRDR-S 134
               ++V++IG A  K+GFFQ+VNHGIP +V+DKM+DG+R FHEQD ++K+Q+YSRDR +
Sbjct: 104 EIRREIVEKIGEASEKYGFFQIVNHGIPQDVMDKMVDGVRKFHEQDDQIKRQYYSRDRFN 163

Query: 135 KKVTHFSNFDLFQTKAANWRDTLSLSMAPNHPLPEEIPEICREIVMEYTKEVKKLGLTLL 194
           K   + SN+ L    A NWRDT+   M  N P P+E P++CR+I+M+Y+  V+ LGL L 
Sbjct: 164 KNFLYSSNYVLIPGIACNWRDTMECIMNSNQPDPQEFPDVCRDILMKYSNYVRNLGLILF 223

Query: 195 ELISKALGLQARHLKELGCADGLLMFGHYYPACPEPELTVGTSNHTDVDFITILLQDQIG 254
           EL+S+ALGL+  HL+E+ CA+GL++ GHYYPACP+PELT GTS H+D  F+TIL+QDQIG
Sbjct: 224 ELLSEALGLKPNHLEEMDCAEGLILLGHYYPACPQPELTFGTSKHSDSGFLTILMQDQIG 283

Query: 255 GLQVLHQNRWFDVPPLHGALVINLGDFLQIISNDRMVSVSHRVVAKKLGPRISVASLFRP 314
           GLQ+L +N+W DVP + GALVIN+ D LQ+I+ND+  SV HRV+A K+GPRISVA  F  
Sbjct: 284 GLQILLENQWIDVPFIPGALVINIADLLQLITNDKFKSVEHRVLANKVGPRISVAVAFGI 343

Query: 315 YIGEDVGNS-NVYEPIKELLSEDNPPIYKQVDPKEYIKLHYSKGLDGSSKLELFKL 359
                 G S  +Y PIKEL+SE+NPPIYK+V  K++I + ++K  D SS L  F+L
Sbjct: 344 KTQTQEGVSPRLYGPIKELISEENPPIYKEVTVKDFITIRFAKRFDDSSSLSPFRL 399

BLAST of Sgr005287 vs. ExPASy Swiss-Prot
Match: Q9LTH7 (1-aminocyclopropane-1-carboxylate oxidase homolog 12 OS=Arabidopsis thaliana OX=3702 GN=At5g59540 PE=2 SV=1)

HSP 1 Score: 373.2 bits (957), Expect = 3.2e-102
Identity = 182/357 (50.98%), Postives = 253/357 (70.87%), Query Frame = 0

Query: 15  ELESFDNSKAGVKGLLDAGITKIPRIFHCDKQDV-----NASAESELSIPIIDL------ 74
           E ++FD +K GVKGL+DA IT++PRIFH  +QD+      +++ S+L IPIID       
Sbjct: 14  ERKAFDETKQGVKGLVDAKITEVPRIFH-HRQDILTNKKPSASVSDLEIPIIDFASVHAD 73

Query: 75  QGGGEQVVDQIGSACRKWGFFQVVNHGIPVEVLDKMIDGIRGFHEQDLELKKQFYSRDR- 134
               E +V+++  A   WGFFQV+NH IP+ VL+++ DG+R FHE+D E+KK F+SRD  
Sbjct: 74  TASREAIVEKVKYAVENWGFFQVINHSIPLNVLEEIKDGVRRFHEEDPEVKKSFFSRDAG 133

Query: 135 SKKVTHFSNFDLFQTK-AANWRDTLSLSMAPNHPLPEEIPEICREIVMEYTKEVKKLGLT 194
           +KK  + SNFDL+ +  + NWRD+ S  +AP+ P PEEIPE CR+ + EY+K V   G  
Sbjct: 134 NKKFVYNSNFDLYSSSPSVNWRDSFSCYIAPDPPAPEEIPETCRDAMFEYSKHVLSFGGL 193

Query: 195 LLELISKALGLQARHLKELGCADGLLMFGHYYPACPEPELTVGTSNHTDVDFITILLQDQ 254
           L EL+S+ALGL+++ L+ + C   LLM  HYYP CP+P+LT+G + H+D  F+T+LLQD 
Sbjct: 194 LFELLSEALGLKSQTLESMDCVKTLLMICHYYPPCPQPDLTLGITKHSDNSFLTLLLQDN 253

Query: 255 IGGLQVLHQNRWFDVPPLHGALVINLGDFLQIISNDRMVSVSHRVVAKKLGPRISVASLF 314
           IGGLQ+LHQ+ W DV P+HGALV+N+GDFLQ+I+ND+ VSV HRV+A + GPRISVAS F
Sbjct: 254 IGGLQILHQDSWVDVSPIHGALVVNIGDFLQLITNDKFVSVEHRVLANRQGPRISVASFF 313

Query: 315 RPYIGEDVGNSNVYEPIKELLSEDNPPIYKQVDPKEYIKLHYSKGLDGSSKLELFKL 359
              +     NS VY P+KEL+SE+NPP Y+ +  KEY K+ + KGLDG+S L   ++
Sbjct: 314 SSSMRP---NSRVYGPMKELVSEENPPKYRDITIKEYSKIFFEKGLDGTSHLSNIRI 366

BLAST of Sgr005287 vs. ExPASy Swiss-Prot
Match: Q8H1S4 (1-aminocyclopropane-1-carboxylate oxidase homolog 3 OS=Arabidopsis thaliana OX=3702 GN=At1g06650 PE=2 SV=1)

HSP 1 Score: 372.9 bits (956), Expect = 4.2e-102
Identity = 181/361 (50.14%), Postives = 252/361 (69.81%), Query Frame = 0

Query: 12  RRTELESFDNSKAGVKGLLDAGITKIPRIFH--CDKQDVNASAESEL----SIPIIDLQG 71
           R +EL++FD +K GVKGL+D+G++++PRIFH    K        S+L    +IP IDL G
Sbjct: 12  RASELKAFDETKTGVKGLVDSGVSQVPRIFHHPTVKLSTPKPLPSDLLHLKTIPTIDLGG 71

Query: 72  GGEQ-------VVDQIGSACRKWGFFQVVNHGIPVEVLDKMIDGIRGFHEQDLELKKQFY 131
              Q        +++I  A  KWGFFQV+NHG+ +E+L+KM  G+R FHEQ  E++K+FY
Sbjct: 72  RDFQDAIKRNNAIEEIKEAAAKWGFFQVINHGVSLELLEKMKKGVRDFHEQSQEVRKEFY 131

Query: 132 SRDRSKKVTHFSNFDLFQTKAANWRDTLSLSMAPNHPLPEEIPEICREIVMEYTKEVKKL 191
           SRD S++  + SNFDLF + AANWRDT S +MAP+ P P+++PEICR+I+MEY+K+V  L
Sbjct: 132 SRDFSRRFLYLSNFDLFSSPAANWRDTFSCTMAPDTPKPQDLPEICRDIMMEYSKQVMNL 191

Query: 192 GLTLLELISKALGLQARHLKELGCADGLLMFGHYYPACPEPELTVGTSNHTDVDFITILL 251
           G  L EL+S+ALGL+  HL ++ C+ GLLM  HYYP CPEP+LT+GTS H+D  F+T+LL
Sbjct: 192 GKFLFELLSEALGLEPNHLNDMDCSKGLLMLSHYYPPCPEPDLTLGTSQHSDNSFLTVLL 251

Query: 252 QDQIGGLQVLHQNRWFDVPPLHGALVINLGDFLQIISNDRMVSVSHRVVAKK-LGPRISV 311
            DQI GLQV  +  WFDVP + GAL+IN+GD LQ+I+ND+ +S+ HRV+A +    R+SV
Sbjct: 252 PDQIEGLQVRREGHWFDVPHVSGALIINIGDLLQLITNDKFISLEHRVLANRATRARVSV 311

Query: 312 ASLFRPYIGEDVGNSNVYEPIKELLSEDNPPIYKQVDPKEYIKLHYSKGLDGSSKLELFK 359
           A  F   +     N  +Y PI+EL+SE+NPP Y++   K+Y     +KGLDG+S L  FK
Sbjct: 312 ACFFTTGVRP---NPRMYGPIRELVSEENPPKYRETTIKDYATYFNAKGLDGTSALLHFK 369

BLAST of Sgr005287 vs. ExPASy Swiss-Prot
Match: Q9LTH8 (1-aminocyclopropane-1-carboxylate oxidase homolog 11 OS=Arabidopsis thaliana OX=3702 GN=At5g59530 PE=2 SV=1)

HSP 1 Score: 368.6 bits (945), Expect = 7.9e-101
Identity = 187/369 (50.68%), Postives = 254/369 (68.83%), Query Frame = 0

Query: 1   MAANGTESFNSRRTELESFDNSKAGVKGLLDAGITKIPRIFHCDKQDV--NASAESELSI 60
           MA N  E    R  E ++FDN+K GVKGL+DA IT+IPRIFH  +  +     + S+L I
Sbjct: 1   MAKNSVEF--DRYIERKAFDNTKEGVKGLIDAKITEIPRIFHVPQDTLPDKKRSVSDLEI 60

Query: 61  PIIDLQG------GGEQVVDQIGSACRKWGFFQVVNHGIPVEVLDKMIDGIRGFH-EQDL 120
           P ID           E +V+++  A   WGFFQV+NHG+P+ VL+++ DG+R FH E+D 
Sbjct: 61  PTIDFASVNVDTPSREAIVEKVKYAVENWGFFQVINHGVPLNVLEEIKDGVRRFHEEEDP 120

Query: 121 ELKKQFYSRDRSK-KVTHFSNFDLFQTK-AANWRDTLSLSMAPNHPLPEEIPEICREIVM 180
           E+KK +YS D +K K  + SNFDL+ +  +  WRD++S  MAP+ P PEE+PE CR+ ++
Sbjct: 121 EVKKSYYSLDFTKNKFAYSSNFDLYSSSPSLTWRDSISCYMAPDPPTPEELPETCRDAMI 180

Query: 181 EYTKEVKKLGLTLLELISKALGLQARHLKELGCADGLLMFGHYYPACPEPELTVGTSNHT 240
           EY+K V  LG  L EL+S+ALGL++  LK + C   LLM  HYYP CP+P+LT+G S H+
Sbjct: 181 EYSKHVLSLGDLLFELLSEALGLKSEILKSMDCLKSLLMICHYYPPCPQPDLTLGISKHS 240

Query: 241 DVDFITILLQDQIGGLQVLHQNRWFDVPPLHGALVINLGDFLQIISNDRMVSVSHRVVAK 300
           D  F+T+LLQD IGGLQ+LHQ+ W DV PL GALV+N+GDFLQ+I+ND+ +SV HRV+A 
Sbjct: 241 DNSFLTVLLQDNIGGLQILHQDSWVDVSPLPGALVVNVGDFLQLITNDKFISVEHRVLAN 300

Query: 301 KLGPRISVASLFRPYIGEDVGNSNVYEPIKELLSEDNPPIYKQVDPKEYIKLHYSKGLDG 359
             GPRISVAS F   I E   NS VY P+KEL+SE+NPP Y+    +EY + ++ KGLDG
Sbjct: 301 TRGPRISVASFFSSSIRE---NSTVYGPMKELVSEENPPKYRDTTLREYSEGYFKKGLDG 360

BLAST of Sgr005287 vs. ExPASy TrEMBL
Match: A0A6J1CAP5 (1-aminocyclopropane-1-carboxylate oxidase homolog 1-like OS=Momordica charantia OX=3673 GN=LOC111009755 PE=3 SV=1)

HSP 1 Score: 622.9 bits (1605), Expect = 8.6e-175
Identity = 301/354 (85.03%), Postives = 319/354 (90.11%), Query Frame = 0

Query: 5   GTESFNSRRTELESFDNSKAGVKGLLDAGITKIPRIFHCDKQDVNASAESELSIPIIDLQ 64
           GTES +SRR ELESFDNSKAG KGL DAGITKIP IFHC KQD+NA+AESELSIPIIDL 
Sbjct: 7   GTESLSSRRAELESFDNSKAGAKGLSDAGITKIPPIFHCQKQDLNAAAESELSIPIIDLH 66

Query: 65  GGGEQVVDQIGSACRKWGFFQVVNHGIPVEVLDKMIDGIRGFHEQDLELKKQFYSRDRSK 124
           GG E V+DQIGSACRKWGFFQVVNHGIPVEVLD MIDGIRGFHEQDLE KKQFYSRDR K
Sbjct: 67  GGEEVVMDQIGSACRKWGFFQVVNHGIPVEVLDGMIDGIRGFHEQDLEWKKQFYSRDRGK 126

Query: 125 KVTHFSNFDLFQTKAANWRDTLSLSMAPNHPLPEEIPEICREIVMEYTKEVKKLGLTLLE 184
           KV +FSNFDLFQ+KAANW+DTLSLSMAP HP P EIPEICREIV+EYTKEVKKLG  LLE
Sbjct: 127 KVAYFSNFDLFQSKAANWKDTLSLSMAPIHPDPHEIPEICREIVIEYTKEVKKLGWILLE 186

Query: 185 LISKALGLQARHLKELGCADGLLMFGHYYPACPEPELTVGTSNHTDVDFITILLQDQIGG 244
           LISKALGL+  HLK++GCADG+LM  HYYP CPEP LT+GT+NHTDV FITILLQDQIGG
Sbjct: 187 LISKALGLEGSHLKDIGCADGVLMLSHYYPPCPEPHLTIGTANHTDVAFITILLQDQIGG 246

Query: 245 LQVLHQNRWFDVPPLHGALVINLGDFLQIISNDRMVSVSHRVVAKKLGPRISVASLFRPY 304
           LQVLH NRW  VPPLHGALVINLGDFLQI+SND MVSVSHRV AKK+GPR+SVAS FRPY
Sbjct: 247 LQVLHHNRWLHVPPLHGALVINLGDFLQIVSNDAMVSVSHRVAAKKIGPRVSVASQFRPY 306

Query: 305 IGEDVGNSNVYEPIKELLSEDNPPIYKQVDPKEYIKLHYSKGLDGSSKLELFKL 359
           +GEDVGNS VYEPIKELLSEDNPP YKQVDPKEYIKLH SKG DGSSKLELFKL
Sbjct: 307 VGEDVGNSKVYEPIKELLSEDNPPKYKQVDPKEYIKLHASKGFDGSSKLELFKL 360

BLAST of Sgr005287 vs. ExPASy TrEMBL
Match: A0A6J1EQU3 (1-aminocyclopropane-1-carboxylate oxidase homolog 1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111436859 PE=3 SV=1)

HSP 1 Score: 581.3 bits (1497), Expect = 2.9e-162
Identity = 279/360 (77.50%), Postives = 322/360 (89.44%), Query Frame = 0

Query: 1   MAANGTESFNSRRTELESFDNSKAGVKGLLDAGITKIPRIFHC--DKQDVNASAESELSI 60
           MAA  TES NSRR++LE+F+N+K+GVKGLLDAGITKIP IFHC        A+ ESE SI
Sbjct: 52  MAAIKTESLNSRRSQLEAFENTKSGVKGLLDAGITKIPSIFHCHVKPHTKTAATESESSI 111

Query: 61  PIIDLQGGGEQVVDQIGSACRKWGFFQVVNHGIPVEVLDKMIDGIRGFHEQDLELKKQFY 120
           PIIDL G  E+VVD+I +ACRKWGFFQVVNHGIPVEVL+++I+G+RGFHEQD ELKKQFY
Sbjct: 112 PIIDLHGSVEEVVDEIRNACRKWGFFQVVNHGIPVEVLEQVINGVRGFHEQDPELKKQFY 171

Query: 121 SRDRSKKVTHFSNFDLFQTKAANWRDTLSLSMAPNHPLPEEIPEICREIVMEYTKEVKKL 180
           SRDR+KKV++FSNFDLFQ+KAANWRDTLS SMAP+ PLPEEIP+ICREIVMEYT++VKKL
Sbjct: 172 SRDRTKKVSYFSNFDLFQSKAANWRDTLSFSMAPSLPLPEEIPQICREIVMEYTEQVKKL 231

Query: 181 GLTLLELISKALGLQARHLKELGCADGLLMFGHYYPACPEPELTVGTSNHTDVDFITILL 240
           GLTL ELISKAL L   HLK++GCA+GLLM GHYYPACPEP+LT+GT+NHTDV FITILL
Sbjct: 232 GLTLFELISKALELGDTHLKDMGCAEGLLMLGHYYPACPEPDLTLGTTNHTDVAFITILL 291

Query: 241 QDQIGGLQVLHQNRWFDVPPLHGALVINLGDFLQIISNDRMVSVSHRVVAKKLGPRISVA 300
           QDQIGGLQV H N+WFDVPPLHGALVIN GDFLQIISND+MVSVSHRVVAK++GPR S+A
Sbjct: 292 QDQIGGLQVFHDNQWFDVPPLHGALVINAGDFLQIISNDKMVSVSHRVVAKEIGPRTSIA 351

Query: 301 SLFRPYIGEDVGNSNVYEPIKELLSEDNPPIYKQVDPKEYIKLHYSKGLDGSSKLELFKL 359
           S FRPY+G+DV NS +YEPIKELLSEDNPPIY+QV+PKE+I+LH+S GLD  SKL+LFKL
Sbjct: 352 SQFRPYMGKDVKNSKLYEPIKELLSEDNPPIYRQVNPKEFIELHFSNGLDECSKLDLFKL 411

BLAST of Sgr005287 vs. ExPASy TrEMBL
Match: A0A6J1EQD8 (1-aminocyclopropane-1-carboxylate oxidase homolog 1-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111436859 PE=3 SV=1)

HSP 1 Score: 576.6 bits (1485), Expect = 7.0e-161
Identity = 279/361 (77.29%), Postives = 322/361 (89.20%), Query Frame = 0

Query: 1   MAANGTESFNSRRTELESFDNSKAGVKGLLDAGITKIPRIFHC--DKQDVNASAESELSI 60
           MAA  TES NSRR++LE+F+N+K+GVKGLLDAGITKIP IFHC        A+ ESE SI
Sbjct: 21  MAAIKTESLNSRRSQLEAFENTKSGVKGLLDAGITKIPSIFHCHVKPHTKTAATESESSI 80

Query: 61  PIIDLQGGGEQVVDQIGSACRKWGFFQVVNHGIPVEVLDKMIDGIRGFHEQDLELKKQFY 120
           PIIDL G  E+VVD+I +ACRKWGFFQVVNHGIPVEVL+++I+G+RGFHEQD ELKKQFY
Sbjct: 81  PIIDLHGSVEEVVDEIRNACRKWGFFQVVNHGIPVEVLEQVINGVRGFHEQDPELKKQFY 140

Query: 121 SRDRSKKVTHFSNFDLFQTKAANWRDTLSLSMAPNHPLPEEIPEICREIVMEYTKEVKKL 180
           SRDR+KKV++FSNFDLFQ+KAANWRDTLS SMAP+ PLPEEIP+ICREIVMEYT++VKKL
Sbjct: 141 SRDRTKKVSYFSNFDLFQSKAANWRDTLSFSMAPSLPLPEEIPQICREIVMEYTEQVKKL 200

Query: 181 GLTLLELISKALGLQARHLKELGCADGLLMFGHYYPACPEPELTVGTSNHTDVDFITILL 240
           GLTL ELISKAL L   HLK++GCA+GLLM GHYYPACPEP+LT+GT+NHTDV FITILL
Sbjct: 201 GLTLFELISKALELGDTHLKDMGCAEGLLMLGHYYPACPEPDLTLGTTNHTDVAFITILL 260

Query: 241 QDQIGGLQVLHQNRWFDVPPLHGALVINLGDFL-QIISNDRMVSVSHRVVAKKLGPRISV 300
           QDQIGGLQV H N+WFDVPPLHGALVIN GDFL QIISND+MVSVSHRVVAK++GPR S+
Sbjct: 261 QDQIGGLQVFHDNQWFDVPPLHGALVINAGDFLQQIISNDKMVSVSHRVVAKEIGPRTSI 320

Query: 301 ASLFRPYIGEDVGNSNVYEPIKELLSEDNPPIYKQVDPKEYIKLHYSKGLDGSSKLELFK 359
           AS FRPY+G+DV NS +YEPIKELLSEDNPPIY+QV+PKE+I+LH+S GLD  SKL+LFK
Sbjct: 321 ASQFRPYMGKDVKNSKLYEPIKELLSEDNPPIYRQVNPKEFIELHFSNGLDECSKLDLFK 380

BLAST of Sgr005287 vs. ExPASy TrEMBL
Match: A0A6J1EV24 (1-aminocyclopropane-1-carboxylate oxidase homolog 1-like isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC111436859 PE=3 SV=1)

HSP 1 Score: 576.6 bits (1485), Expect = 7.0e-161
Identity = 279/361 (77.29%), Postives = 322/361 (89.20%), Query Frame = 0

Query: 1   MAANGTESFNSRRTELESFDNSKAGVKGLLDAGITKIPRIFHC--DKQDVNASAESELSI 60
           MAA  TES NSRR++LE+F+N+K+GVKGLLDAGITKIP IFHC        A+ ESE SI
Sbjct: 1   MAAIKTESLNSRRSQLEAFENTKSGVKGLLDAGITKIPSIFHCHVKPHTKTAATESESSI 60

Query: 61  PIIDLQGGGEQVVDQIGSACRKWGFFQVVNHGIPVEVLDKMIDGIRGFHEQDLELKKQFY 120
           PIIDL G  E+VVD+I +ACRKWGFFQVVNHGIPVEVL+++I+G+RGFHEQD ELKKQFY
Sbjct: 61  PIIDLHGSVEEVVDEIRNACRKWGFFQVVNHGIPVEVLEQVINGVRGFHEQDPELKKQFY 120

Query: 121 SRDRSKKVTHFSNFDLFQTKAANWRDTLSLSMAPNHPLPEEIPEICREIVMEYTKEVKKL 180
           SRDR+KKV++FSNFDLFQ+KAANWRDTLS SMAP+ PLPEEIP+ICREIVMEYT++VKKL
Sbjct: 121 SRDRTKKVSYFSNFDLFQSKAANWRDTLSFSMAPSLPLPEEIPQICREIVMEYTEQVKKL 180

Query: 181 GLTLLELISKALGLQARHLKELGCADGLLMFGHYYPACPEPELTVGTSNHTDVDFITILL 240
           GLTL ELISKAL L   HLK++GCA+GLLM GHYYPACPEP+LT+GT+NHTDV FITILL
Sbjct: 181 GLTLFELISKALELGDTHLKDMGCAEGLLMLGHYYPACPEPDLTLGTTNHTDVAFITILL 240

Query: 241 QDQIGGLQVLHQNRWFDVPPLHGALVINLGDFL-QIISNDRMVSVSHRVVAKKLGPRISV 300
           QDQIGGLQV H N+WFDVPPLHGALVIN GDFL QIISND+MVSVSHRVVAK++GPR S+
Sbjct: 241 QDQIGGLQVFHDNQWFDVPPLHGALVINAGDFLQQIISNDKMVSVSHRVVAKEIGPRTSI 300

Query: 301 ASLFRPYIGEDVGNSNVYEPIKELLSEDNPPIYKQVDPKEYIKLHYSKGLDGSSKLELFK 359
           AS FRPY+G+DV NS +YEPIKELLSEDNPPIY+QV+PKE+I+LH+S GLD  SKL+LFK
Sbjct: 301 ASQFRPYMGKDVKNSKLYEPIKELLSEDNPPIYRQVNPKEFIELHFSNGLDECSKLDLFK 360

BLAST of Sgr005287 vs. ExPASy TrEMBL
Match: A0A6J1EWT9 (1-aminocyclopropane-1-carboxylate oxidase homolog 1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111436859 PE=3 SV=1)

HSP 1 Score: 576.6 bits (1485), Expect = 7.0e-161
Identity = 279/361 (77.29%), Postives = 322/361 (89.20%), Query Frame = 0

Query: 1   MAANGTESFNSRRTELESFDNSKAGVKGLLDAGITKIPRIFHC--DKQDVNASAESELSI 60
           MAA  TES NSRR++LE+F+N+K+GVKGLLDAGITKIP IFHC        A+ ESE SI
Sbjct: 52  MAAIKTESLNSRRSQLEAFENTKSGVKGLLDAGITKIPSIFHCHVKPHTKTAATESESSI 111

Query: 61  PIIDLQGGGEQVVDQIGSACRKWGFFQVVNHGIPVEVLDKMIDGIRGFHEQDLELKKQFY 120
           PIIDL G  E+VVD+I +ACRKWGFFQVVNHGIPVEVL+++I+G+RGFHEQD ELKKQFY
Sbjct: 112 PIIDLHGSVEEVVDEIRNACRKWGFFQVVNHGIPVEVLEQVINGVRGFHEQDPELKKQFY 171

Query: 121 SRDRSKKVTHFSNFDLFQTKAANWRDTLSLSMAPNHPLPEEIPEICREIVMEYTKEVKKL 180
           SRDR+KKV++FSNFDLFQ+KAANWRDTLS SMAP+ PLPEEIP+ICREIVMEYT++VKKL
Sbjct: 172 SRDRTKKVSYFSNFDLFQSKAANWRDTLSFSMAPSLPLPEEIPQICREIVMEYTEQVKKL 231

Query: 181 GLTLLELISKALGLQARHLKELGCADGLLMFGHYYPACPEPELTVGTSNHTDVDFITILL 240
           GLTL ELISKAL L   HLK++GCA+GLLM GHYYPACPEP+LT+GT+NHTDV FITILL
Sbjct: 232 GLTLFELISKALELGDTHLKDMGCAEGLLMLGHYYPACPEPDLTLGTTNHTDVAFITILL 291

Query: 241 QDQIGGLQVLHQNRWFDVPPLHGALVINLGDFL-QIISNDRMVSVSHRVVAKKLGPRISV 300
           QDQIGGLQV H N+WFDVPPLHGALVIN GDFL QIISND+MVSVSHRVVAK++GPR S+
Sbjct: 292 QDQIGGLQVFHDNQWFDVPPLHGALVINAGDFLQQIISNDKMVSVSHRVVAKEIGPRTSI 351

Query: 301 ASLFRPYIGEDVGNSNVYEPIKELLSEDNPPIYKQVDPKEYIKLHYSKGLDGSSKLELFK 359
           AS FRPY+G+DV NS +YEPIKELLSEDNPPIY+QV+PKE+I+LH+S GLD  SKL+LFK
Sbjct: 352 ASQFRPYMGKDVKNSKLYEPIKELLSEDNPPIYRQVNPKEFIELHFSNGLDECSKLDLFK 411

BLAST of Sgr005287 vs. TAIR 10
Match: AT1G06620.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 412.9 bits (1060), Expect = 2.6e-115
Identity = 198/356 (55.62%), Postives = 255/356 (71.63%), Query Frame = 0

Query: 12  RRTELESFDNSKAGVKGLLDAGITKIPRIFHCDKQDVNA---SAESELSIPIIDLQGGG- 71
           R T L++FD +K GVKGL+DAGIT+IP IF      + +    + S+ SIP IDL+GGG 
Sbjct: 13  RSTLLKAFDETKTGVKGLIDAGITEIPSIFRAPPATLTSPKPPSSSDFSIPTIDLKGGGT 72

Query: 72  -----EQVVDQIGSACRKWGFFQVVNHGIPVEVLDKMIDGIRGFHEQDLELKKQFYSRDR 131
                  +V++IG A  KWGFFQV+NHGIP++VL+KMIDGIR FHEQD E+KK FYSRD 
Sbjct: 73  DSITRRSLVEKIGDAAEKWGFFQVINHGIPMDVLEKMIDGIREFHEQDTEVKKGFYSRDP 132

Query: 132 SKKVTHFSNFDLFQTKAANWRDTLSLSMAPNHPLPEEIPEICREIVMEYTKEVKKLGLTL 191
           + K+ + SNFDLF + AANWRDTL    AP+ P PE++P  C E+++EY+KEV KLG  L
Sbjct: 133 ASKMVYSSNFDLFSSPAANWRDTLGCYTAPDPPRPEDLPATCGEMMIEYSKEVMKLGKLL 192

Query: 192 LELISKALGLQARHLKELGCADGLLMFGHYYPACPEPELTVGTSNHTDVDFITILLQDQI 251
            EL+S+ALGL   HLK++ C + LL+ GHYYP CP+P+LT+G + H+D  F+TILLQD I
Sbjct: 193 FELLSEALGLNTNHLKDMDCTNSLLLLGHYYPPCPQPDLTLGLTKHSDNSFLTILLQDHI 252

Query: 252 GGLQVLHQNRWFDVPPLHGALVINLGDFLQIISNDRMVSVSHRVVAKKLGPRISVASLFR 311
           GGLQVLH   W DVPP+ GALV+N+GD LQ+I+ND+ +SV HRV+A   GPRISVA  F 
Sbjct: 253 GGLQVLHDQYWVDVPPVPGALVVNVGDLLQLITNDKFISVEHRVLANVAGPRISVACFFS 312

Query: 312 PYIGEDVGNSNVYEPIKELLSEDNPPIYKQVDPKEYIKLHYSKGLDGSSKLELFKL 359
            Y+   + N  VY PIKE+LSE+NPP Y+     EY K + SKG DG+S L   K+
Sbjct: 313 SYL---MANPRVYGPIKEILSEENPPNYRDTTITEYAKFYRSKGFDGTSGLLYLKI 365

BLAST of Sgr005287 vs. TAIR 10
Match: AT2G30830.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 385.6 bits (989), Expect = 4.4e-107
Identity = 194/358 (54.19%), Postives = 252/358 (70.39%), Query Frame = 0

Query: 12  RRTELESFDNSKAGVKGLLDAGITKIPRIFHCDKQDV---NASAESELSIPIIDLQGG-- 71
           R  E+++FD  K GVKGL+DAGITK+PRIFH   QDV   N    S L IP ID+ GG  
Sbjct: 7   RAGEVKAFDQMKIGVKGLVDAGITKVPRIFH--HQDVAVTNPKPSSTLEIPTIDVGGGVF 66

Query: 72  -----GEQVVDQIGSACRKWGFFQVVNHGIPVEVLDKMIDGIRGFHEQDLELKKQFYSRD 131
                 + V+ ++ +A  K+GFFQV+NHGIP+EV++ M DGIRGFHEQD E+KK FYSRD
Sbjct: 67  ESTVTRKSVIAKVRAAVEKFGFFQVINHGIPLEVMESMKDGIRGFHEQDSEVKKTFYSRD 126

Query: 132 RSKKVTHFSNFDLFQTKAANWRDTLSLSMAPNHPLPEEIPEICREIVMEYTKEVKKLGLT 191
            +KKV + +NFDL+ ++AANWRDTL++ MAP+ P   ++P ICREI++EY+K + KLG  
Sbjct: 127 ITKKVKYNTNFDLYSSQAANWRDTLTMVMAPDVPQAGDLPVICREIMLEYSKRMMKLGEL 186

Query: 192 LLELISKALGLQARHLKELGCADGLLMFGHYYPACPEPELTVGTSNHTDVDFITILLQDQ 251
           + EL+S+ALGL+  HLKEL CA  L +  HYYP CPEP+ T G S+HTD+ FITILLQD 
Sbjct: 187 IFELLSEALGLKPNHLKELNCAKSLSLLSHYYPPCPEPDRTFGISSHTDISFITILLQDH 246

Query: 252 IGGLQVLHQNRWFDVPPLHGALVINLGDFLQIISNDRMVSVSHRVVAKK-LGPRISVASL 311
           IGGLQVLH   W DVPP   AL++NLGD LQ+I+ND+ VSV HRV+A +   PRIS AS 
Sbjct: 247 IGGLQVLHDGYWIDVPPNPEALIVNLGDLLQLITNDKFVSVEHRVLANRGEEPRISSASF 306

Query: 312 FRPYIGEDVGNSNVYEPIKELLSEDNPPIYKQVDPKEYIKLHYSKGLDGSSKLELFKL 359
           F       + N  VY P+KELLS+ NPP Y+     E  + + ++GLDG+S L  F++
Sbjct: 307 FM----HTIPNEQVYGPMKELLSKQNPPKYRNTTTTEMARHYLARGLDGTSPLLHFRI 358

BLAST of Sgr005287 vs. TAIR 10
Match: AT5G59540.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 373.2 bits (957), Expect = 2.3e-103
Identity = 182/357 (50.98%), Postives = 253/357 (70.87%), Query Frame = 0

Query: 15  ELESFDNSKAGVKGLLDAGITKIPRIFHCDKQDV-----NASAESELSIPIIDL------ 74
           E ++FD +K GVKGL+DA IT++PRIFH  +QD+      +++ S+L IPIID       
Sbjct: 14  ERKAFDETKQGVKGLVDAKITEVPRIFH-HRQDILTNKKPSASVSDLEIPIIDFASVHAD 73

Query: 75  QGGGEQVVDQIGSACRKWGFFQVVNHGIPVEVLDKMIDGIRGFHEQDLELKKQFYSRDR- 134
               E +V+++  A   WGFFQV+NH IP+ VL+++ DG+R FHE+D E+KK F+SRD  
Sbjct: 74  TASREAIVEKVKYAVENWGFFQVINHSIPLNVLEEIKDGVRRFHEEDPEVKKSFFSRDAG 133

Query: 135 SKKVTHFSNFDLFQTK-AANWRDTLSLSMAPNHPLPEEIPEICREIVMEYTKEVKKLGLT 194
           +KK  + SNFDL+ +  + NWRD+ S  +AP+ P PEEIPE CR+ + EY+K V   G  
Sbjct: 134 NKKFVYNSNFDLYSSSPSVNWRDSFSCYIAPDPPAPEEIPETCRDAMFEYSKHVLSFGGL 193

Query: 195 LLELISKALGLQARHLKELGCADGLLMFGHYYPACPEPELTVGTSNHTDVDFITILLQDQ 254
           L EL+S+ALGL+++ L+ + C   LLM  HYYP CP+P+LT+G + H+D  F+T+LLQD 
Sbjct: 194 LFELLSEALGLKSQTLESMDCVKTLLMICHYYPPCPQPDLTLGITKHSDNSFLTLLLQDN 253

Query: 255 IGGLQVLHQNRWFDVPPLHGALVINLGDFLQIISNDRMVSVSHRVVAKKLGPRISVASLF 314
           IGGLQ+LHQ+ W DV P+HGALV+N+GDFLQ+I+ND+ VSV HRV+A + GPRISVAS F
Sbjct: 254 IGGLQILHQDSWVDVSPIHGALVVNIGDFLQLITNDKFVSVEHRVLANRQGPRISVASFF 313

Query: 315 RPYIGEDVGNSNVYEPIKELLSEDNPPIYKQVDPKEYIKLHYSKGLDGSSKLELFKL 359
              +     NS VY P+KEL+SE+NPP Y+ +  KEY K+ + KGLDG+S L   ++
Sbjct: 314 SSSMRP---NSRVYGPMKELVSEENPPKYRDITIKEYSKIFFEKGLDGTSHLSNIRI 366

BLAST of Sgr005287 vs. TAIR 10
Match: AT1G06650.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 372.9 bits (956), Expect = 3.0e-103
Identity = 181/361 (50.14%), Postives = 252/361 (69.81%), Query Frame = 0

Query: 12  RRTELESFDNSKAGVKGLLDAGITKIPRIFH--CDKQDVNASAESEL----SIPIIDLQG 71
           R +EL++FD +K GVKGL+D+G++++PRIFH    K        S+L    +IP IDL G
Sbjct: 12  RASELKAFDETKTGVKGLVDSGVSQVPRIFHHPTVKLSTPKPLPSDLLHLKTIPTIDLGG 71

Query: 72  GGEQ-------VVDQIGSACRKWGFFQVVNHGIPVEVLDKMIDGIRGFHEQDLELKKQFY 131
              Q        +++I  A  KWGFFQV+NHG+ +E+L+KM  G+R FHEQ  E++K+FY
Sbjct: 72  RDFQDAIKRNNAIEEIKEAAAKWGFFQVINHGVSLELLEKMKKGVRDFHEQSQEVRKEFY 131

Query: 132 SRDRSKKVTHFSNFDLFQTKAANWRDTLSLSMAPNHPLPEEIPEICREIVMEYTKEVKKL 191
           SRD S++  + SNFDLF + AANWRDT S +MAP+ P P+++PEICR+I+MEY+K+V  L
Sbjct: 132 SRDFSRRFLYLSNFDLFSSPAANWRDTFSCTMAPDTPKPQDLPEICRDIMMEYSKQVMNL 191

Query: 192 GLTLLELISKALGLQARHLKELGCADGLLMFGHYYPACPEPELTVGTSNHTDVDFITILL 251
           G  L EL+S+ALGL+  HL ++ C+ GLLM  HYYP CPEP+LT+GTS H+D  F+T+LL
Sbjct: 192 GKFLFELLSEALGLEPNHLNDMDCSKGLLMLSHYYPPCPEPDLTLGTSQHSDNSFLTVLL 251

Query: 252 QDQIGGLQVLHQNRWFDVPPLHGALVINLGDFLQIISNDRMVSVSHRVVAKK-LGPRISV 311
            DQI GLQV  +  WFDVP + GAL+IN+GD LQ+I+ND+ +S+ HRV+A +    R+SV
Sbjct: 252 PDQIEGLQVRREGHWFDVPHVSGALIINIGDLLQLITNDKFISLEHRVLANRATRARVSV 311

Query: 312 ASLFRPYIGEDVGNSNVYEPIKELLSEDNPPIYKQVDPKEYIKLHYSKGLDGSSKLELFK 359
           A  F   +     N  +Y PI+EL+SE+NPP Y++   K+Y     +KGLDG+S L  FK
Sbjct: 312 ACFFTTGVRP---NPRMYGPIRELVSEENPPKYRETTIKDYATYFNAKGLDGTSALLHFK 369

BLAST of Sgr005287 vs. TAIR 10
Match: AT5G59530.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 368.6 bits (945), Expect = 5.6e-102
Identity = 187/369 (50.68%), Postives = 254/369 (68.83%), Query Frame = 0

Query: 1   MAANGTESFNSRRTELESFDNSKAGVKGLLDAGITKIPRIFHCDKQDV--NASAESELSI 60
           MA N  E    R  E ++FDN+K GVKGL+DA IT+IPRIFH  +  +     + S+L I
Sbjct: 1   MAKNSVEF--DRYIERKAFDNTKEGVKGLIDAKITEIPRIFHVPQDTLPDKKRSVSDLEI 60

Query: 61  PIIDLQG------GGEQVVDQIGSACRKWGFFQVVNHGIPVEVLDKMIDGIRGFH-EQDL 120
           P ID           E +V+++  A   WGFFQV+NHG+P+ VL+++ DG+R FH E+D 
Sbjct: 61  PTIDFASVNVDTPSREAIVEKVKYAVENWGFFQVINHGVPLNVLEEIKDGVRRFHEEEDP 120

Query: 121 ELKKQFYSRDRSK-KVTHFSNFDLFQTK-AANWRDTLSLSMAPNHPLPEEIPEICREIVM 180
           E+KK +YS D +K K  + SNFDL+ +  +  WRD++S  MAP+ P PEE+PE CR+ ++
Sbjct: 121 EVKKSYYSLDFTKNKFAYSSNFDLYSSSPSLTWRDSISCYMAPDPPTPEELPETCRDAMI 180

Query: 181 EYTKEVKKLGLTLLELISKALGLQARHLKELGCADGLLMFGHYYPACPEPELTVGTSNHT 240
           EY+K V  LG  L EL+S+ALGL++  LK + C   LLM  HYYP CP+P+LT+G S H+
Sbjct: 181 EYSKHVLSLGDLLFELLSEALGLKSEILKSMDCLKSLLMICHYYPPCPQPDLTLGISKHS 240

Query: 241 DVDFITILLQDQIGGLQVLHQNRWFDVPPLHGALVINLGDFLQIISNDRMVSVSHRVVAK 300
           D  F+T+LLQD IGGLQ+LHQ+ W DV PL GALV+N+GDFLQ+I+ND+ +SV HRV+A 
Sbjct: 241 DNSFLTVLLQDNIGGLQILHQDSWVDVSPLPGALVVNVGDFLQLITNDKFISVEHRVLAN 300

Query: 301 KLGPRISVASLFRPYIGEDVGNSNVYEPIKELLSEDNPPIYKQVDPKEYIKLHYSKGLDG 359
             GPRISVAS F   I E   NS VY P+KEL+SE+NPP Y+    +EY + ++ KGLDG
Sbjct: 301 TRGPRISVASFFSSSIRE---NSTVYGPMKELVSEENPPKYRDTTLREYSEGYFKKGLDG 360