Sed0028199 (gene) Chayote v1

Overview
NameSed0028199
Typegene
OrganismSechium edule (Chayote v1)
DescriptionMechanosensitive ion channel protein
LocationLG01: 18343444 .. 18349838 (-)
RNA-Seq ExpressionSed0028199
SyntenySed0028199
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCTCTGATTCTTTGACGTCCGCCATTCCCACTCAAAACTGCTTTATTTTTCCGCCATTACTGGCCCCACACGAGCTTCAATCACCGTTCGAGTACATTTCTTCACCAATGAAACCCTCAATAAGCTACTCATTTTCCTAGCTTCATGGCTTCGAAGGAAGAAGTATTTCTCAAGATCGACAGCTTCGGAAATGGGGGCGACAAGAAAATCCAGGGTTTACCAGAGAAATCGCCACAAACCATGAGACGAAGAGGCAAAGAGTTTCCAGAGCCGGAAACTCGCCGGCCCGGCGGCGGCGAGGTTCTCCGGTGCAGTTCCTACGCTTCATTTCGTGGAAGCTCATGGAGCTCGCCGATTAGCAAGAGATCTCGGCTGATGGATCCGCCTGAGGAAGATTCTTCTCAAAAGCTTGAAACCGCCGTGAAAGATGAAGGCAACAACAACAACAACGACGACGACAACAACAATCAGGATGATACAGAAGATATGCCTGAAGAATACGATCAACTGAAGCTAAGCCCCTTCTTTATTTTTCAGTTGGTTCTTTCTGTTTTTGTTATTGTGGGTTTGATTTGCAATCGATGGGTTTCGATCTTGAAGAGGGAGACGATTTGGGGTCTTCCGTTGTGGAAATGGGAACTGACTGTTCTAGCTGTGATCTGTGGGCATTTGGTTTCGATTTTGGCCGTTCGATTGATTGTGAAAATCATCGAGATGAATTTTTTGTTGAGGAAACGTGTTCTGTATTTCGTTTATGGGTTAAGGAATTCTGTTCAAATTTGCTTGTGGGTGTCTTTGATTTTGCTTGTTTGGCATTTCATTTTCAACCACAAGGTTCAGATTAAGACTCAGAGTAAGATTTTACCTTATGTTACCAAGATTTTCATCTGTTTTCTCGTGGGTGCTTCGATTTGGCTTCTGAAAACCCTTGCCATTAAAATTCTGGCTTCGTTTTTTCATGTTAATCACTACTTTGAAAGGCTTAAGGAGGCTTTGTTTAGTCAGTTTGTGATTGTGAGTCTCTCTGGTTTGCCATTGTTCAAGCGGCCGAATACTAAAGATGTGATGATGGGAGACCAGAATGCTGATGGAAGAGAAGAAAGTCCCAAAAGTGGGAGAGTTATATGCAGTGGGAGACTTGTAGATTGCAATCGATCGAGACCGTTGAAGCCCGATTCGGTTAAAGAAGATGAGGAAATCCCAGTTGATCAGTTGAATAAAATGAACCAGAAGAATATATCTGCTTGGAATATGAGGAGAATGATGAATATTATAAGAAAGGGAGCTTTGTTAACTCTTGATGAGAAGATATTGTCAGAGACGAATGAAGAATCATTGTTGCAGATTAGGAGTGAACGGCAGGCGAAAGAGGCAGCTGAGAAGATTTTCCAACGAGTTGTTCAACGTGGGTCAGAGTGAGTTCTTTGTTTTGACTTCTTTTCATGAATATTCAAACCGTGTTTTAAAAGCGTTGAATTATGTTAAACCACCAATCAATCTAAAAGTCTAAGATGGATTGAGTCAAATTTAATTATATCAACCAACAAAAAGGCTCACTTAGATTCTCGCCTAGACTCAAGGCTCAAGTCTAGCGTCTTGCTTATGTCTTGACTGCCTCAGCTAAAAAGGCTCGTGGTATCTTGTGTTAATTGTAAAAAAAAGTTTTTCTAATCGTAAACGAACAAATCCTTAGTGTTTAGTGTTTTATTTTGTTCTTTATTAACTAAAAAAATAATATTTTCTAACCTTTATACGTATACTATAATATTCTCAATCCAATTATGTATAATCTTCTCAATCCTAAACCCTATATGTATATGTATATGTATATGTATATGTGTGTGTATATGTATATATATGAAAATTTACCATTAGTAACACATTCTACCTCTTGTATTATATTTATAGCATAATTTTAAAAATAATTCATCTATAACACATAATTTTCTATCACTAATAGAACTCAAAACATAATTTTCTATCAGTGGTAGAACTCAACCAGTAATAGAACATAATTTTCTATCAGTGATATAACTCAACCAGTAATAGAACATAATTTTCTATCGGTGATAGAACTCAACCAGTGATAAAATAAGTCTACCACTGATTGAATTCGATAAAACTCAATAGTAATAGAACTTAATCTTGATTTTTTTTGCTATAAATGAATCAGTTTTGATTTTGTGTTATAGTTGAAAATATTTTGCCTTTTTTGTTATACATGCAAAATCCCTTCCTTATGTCATCCCTTCCTTATGAATGTATCTCACAAAAAAAGACACCTGCGGTTTTTTGCACCTTGTGTCTAAGCTCCAAACTAGGCTATTGTGTCTCAGTGTCCTATAGGCTTTTAAAAATACTCCATTCAACTTCATTACATGATAGAGTGAGTAGTTTTGTTGCAGCTGTATTTATCTGGAAGATGTAATGCGATTTATGAACAAAGAGGAAGCTCTAATGACCCTGAGTTTATTTGGAACAACCGCTGAAACCCAATGCATTGATGAGTCCTCTTTTAGAGAGTGGATGGTAAGGCCCTGCCCGCCCCGTGTTGTTTTTCTGTGTGATGAAGATGATATGTTCGGAGGTTGAATTACCATTTGGTTCTTGGAATTTGATTTGGTTTCATGTTTTTAAAGTTCATTATTATTGTTATGTTTTAATTAAAGCTTATAAGTATTCCTATCAATGTCAAAGTTATGAAAGTCAAACTTGGTAAGTAGTTTTTGCATGTGACAGATATTCCTTCTCAAAATGTCCATTTCCCATGAGAAATAAAGTCTTCGAGAGTCAAACTTAAAACCTGACTCATTTCATTGACTTTCAACCTGAATACTACATAACCCCTCTCTCACTCCCATATCACACTAAACTCCCATGCCAACACATAGGAAACTTATCGACCACATGTAAGTGTACCTTGCCATGTCAAGATTAATGCTGAACAGGGCATAGCTCAACTAACGTAAGTTTGTGTCATCGACCTTGAAGTTAGAGGTTCAAGTTTCAATGTCATTTATTGTTGAACTCAATATGTGTACATATTTTGTAAGCCCTCAAACATGCTCGATGCTTCAACATCAACCCTTTAATGTTAGAGAAGAAATCACTTATATGTGTGTATACATATAGTATTCTTAACCCAAACATTAATGTAGACTTTTATGTTTTTCTTTGATGGGTCTCAGGTAAATGCATTTAAGCAGAGAAAAGTTCTTGTATTATCTCTCGGTGATGCAAATACAGCTGTTGATGATCTTCACAGCTTGCTGAACATCTTTGTAGCTATAATAATTTTGGTAATCTGGCTTATCATATTAGGAACTCCCGTTCGGCACTTTCTCATCTTCATAACTTCACAGCTTCTACTGCTGGTTTTTATTTTTGGTAACACATGTAAGACAGTCTTCGAAGCTATCATTTTCTTGTTTGTGATGCACCCATTTGACATTGGTGATCGCTGCGAAGTAGACGGTGTCCAGGTAAAATGACTATCCTGTCAAAGAATTTGTATCACTCTGATAGGGTTATTATTAAGCATAATTTAGGGGTATGAGTGCAATTAGTTTTTTTTTTAGTACAAGTTGGGGTAGGGAGACCTTAAACCCTACAAAGAGGGTACTACTACTCTCAATAAATGAGAGCCAAATACTGATTCTCGGAATCGAACCCGTGATCATGGTATTGAATTGAATATCCATCAAACGATGAAGCCATTAGGCCACCGTCCCTTCCCTTTCGGTATGAGCGCAATTAGTTGAGTGTTTGTTATCCTCTTTGGTTATAAATAGGGGTAAGGAGTAAGGGTAGAAATATGCAATTTTATGGTAAAGTCATTTAGGGCTTGAGAGGCTATTTTCAAGAGGGAGGTTCAAGTACCTATCAAATACTTGTTATCTTGTATTTTCTTTATTTTTTTGTTAATCGGATCCTATCACACTCTTACCAATGCATTCTTATCTTCTATGGTTTTGCAGATGATTGTTGAAGAGATGAAGATATTAACAACTGTTTTTCTGAGATATGATAACCAGAAGATCACTTACCCCAACAGTGTGCTGGCTACTAAAGCCATTGGCAATTACTACCGCAGTCCAGACATGGGAGAGGGAGTTGATTTCTATGTCCACATCTCAACTCCATGGGAGAAAGTTGCCCTTTTGAAAGAACGGATAACAAGGTATGCTTTCTAAGTAATCGTAAACAACAATCAAGAATAAAAAGAATAAGACAAAGAACACTCAAATTTACGTGGTGCACTAACTGTATTAGACAGATGAAGGAGGGGAGATCATTTTATTTAGAATCTCTCTTTTCATAAAACCTTAATACATCATTTGGCTCAAATTATTTAGTGCACCTATGTAAGAGAGTTTAGTCAGGGTACCTCTCATAAAAATATATTAATACATCATTTGGTCCAAATAACATAAATTCAATACGAAAGTATAGAATATAACTGACATATTCGTATTGAGTATTCGTAGTGGTAGCACAACATAATTAGTTCAAGTCAACTCAACAGTTTTCTGTTGAATAGAATGGCATTGCACATAAGCCTTTGAATCCATCGCTTGTGGATTTGACTCTCTGATTTGGACTTATCATAGAAATTGTCTGTATCTAAGCATCCATGAATGGTTCTAATCTTCTCTTAGTTGTGGTATACTGATATTGCCATGATAGTGATTTTGAAGGTTTATTAGATTCTACCACTACGATTTTTTTTTTTATTAGATGAGTTCCTAGCATTTCTTTTTCATATATATATTTATATAATGGAATTCTTTTCTTCTCCTTTTCGTGTTAAAATGTCCCCGTACTTCAATAGTTAGTTCATAGAAGAGGAATGAAAAAGGAAAAATATCAATTTGACATGAAAAGTACATTTCTAAATCCTTTTCAAGCCACCAAACACTTTTAAGTGTTTTTTCAAAAAGCACTTTTAAATGTTCAACTTTTTCCGCAACATATTACCCCTAAACATAATGTTTTTGCGATGATGGCAAGAAGCATTCTCATAAATAGCTTCGAAGTAAAGCCAAAGTGAGTTTCCGTTGTACCTGAGATGTACCCTCAACTAAAAGGTCATCAGTTCTATCTCCCACCACATATATTGAGATCAAGAAAAGTAAGTCATAAGAACTTATGTCCAAAGTAGACAACATCATATGAATATAGAGATCGGTGGAGGGTCGATGTCCTCGATGATTTAAAAACCATTCTATAGACGTAGAGAAATGGAATCGAGTTTGCAAAATGTGTAAGCGATTCGACGAATCAATCTGCAGGTACGTAGAGAGCATGAGCGAGCTATGGCATCCAAATCCACAGATTGTGATGAAGGAAATTGAAGATCTGGAGAAGGTGAAGATGTCAGTTTGTGTTACACACAAAATGAACCACCAAGACATGAAAGAGAGATGGAACAGGAGAGCTGTGCTGGTTGAAGAGATTGTCACAATTTTGGAGTCAACTTGACAAAGAACATTAACATCTACAAATGTTGTCGGGGGGGAAGACTGTTTCAAACTTGCCCTTTGTCGGAATTAAGCTCACTCTAAACTGATAAGGGCCATAAGCTGCGTTTTGTGCATATTGTTGTTTTTTAGGAAATGATTTTAAGGTATTTTGTACATTATTATTTGAACTTCAAATTTGAAGGTTAGAATGTATCATCATAGGAAATTTGAATAATGAACAGAATCTTTTAACTTTATTAGATTCCTGAACTGATTGAAGTATTGTCTGAGCTTTTTTTTTTTTTCTTTTGCAGTGGATTTTCGTTTTCGAGGTGGAAATTGGTACATGCAGAAACGAACAAATTCATGATATTTTTTGGGTCATACTTTTACCCTTATATAAAATTAATTTCACATTAGTCTTTTCATGATTAGTAGATTAAGAGTTGATTAATGTGTTTACTAATAACAATGTTTTTGAAATAATATTAGTTTTGTATAAATGCTTTGAAAATAATATTAGTTTTTGTATAAAGTACTAATAGAGGTTTTTTTGTTTTTTTTATAAGTATAAATTGGAGAGAGGGACTAACAAATTTTCAAATAATATAAAACTTAATTAATACCATTTTTATGATTGAATCTATGATCGTAGAGTTAAAATAATTTACTTACTACCAATCAAGATAAATAATACATTACCAATATATTCAGTACCATCAACACATTCAGTACTAATATATAGGTTATAAGTTAAGTAAAAAATTTGTATTACTTGTTAAGTGTGATGCACCAAAGTTTAAAAAAAAAAAGTTATTGAAAATAATTCAATTAAAAAACTCAAAGCAGGTAATAAGAGATAAAATATCATTTTAGTTCTTTCGCTTTTCATTTTGTTTCTTGTAGGTCTATGTTTAGAAAATATACATTTT

mRNA sequence

TTCTCTGATTCTTTGACGTCCGCCATTCCCACTCAAAACTGCTTTATTTTTCCGCCATTACTGGCCCCACACGAGCTTCAATCACCGTTCGAGTACATTTCTTCACCAATGAAACCCTCAATAAGCTACTCATTTTCCTAGCTTCATGGCTTCGAAGGAAGAAGTATTTCTCAAGATCGACAGCTTCGGAAATGGGGGCGACAAGAAAATCCAGGGTTTACCAGAGAAATCGCCACAAACCATGAGACGAAGAGGCAAAGAGTTTCCAGAGCCGGAAACTCGCCGGCCCGGCGGCGGCGAGGTTCTCCGGTGCAGTTCCTACGCTTCATTTCGTGGAAGCTCATGGAGCTCGCCGATTAGCAAGAGATCTCGGCTGATGGATCCGCCTGAGGAAGATTCTTCTCAAAAGCTTGAAACCGCCGTGAAAGATGAAGGCAACAACAACAACAACGACGACGACAACAACAATCAGGATGATACAGAAGATATGCCTGAAGAATACGATCAACTGAAGCTAAGCCCCTTCTTTATTTTTCAGTTGGTTCTTTCTGTTTTTGTTATTGTGGGTTTGATTTGCAATCGATGGGTTTCGATCTTGAAGAGGGAGACGATTTGGGGTCTTCCGTTGTGGAAATGGGAACTGACTGTTCTAGCTGTGATCTGTGGGCATTTGGTTTCGATTTTGGCCGTTCGATTGATTGTGAAAATCATCGAGATGAATTTTTTGTTGAGGAAACGTGTTCTGTATTTCGTTTATGGGTTAAGGAATTCTGTTCAAATTTGCTTGTGGGTGTCTTTGATTTTGCTTGTTTGGCATTTCATTTTCAACCACAAGGTTCAGATTAAGACTCAGAGTAAGATTTTACCTTATGTTACCAAGATTTTCATCTGTTTTCTCGTGGGTGCTTCGATTTGGCTTCTGAAAACCCTTGCCATTAAAATTCTGGCTTCGTTTTTTCATGTTAATCACTACTTTGAAAGGCTTAAGGAGGCTTTGTTTAGTCAGTTTGTGATTGTGAGTCTCTCTGGTTTGCCATTGTTCAAGCGGCCGAATACTAAAGATGTGATGATGGGAGACCAGAATGCTGATGGAAGAGAAGAAAGTCCCAAAAGTGGGAGAGTTATATGCAGTGGGAGACTTGTAGATTGCAATCGATCGAGACCGTTGAAGCCCGATTCGGTTAAAGAAGATGAGGAAATCCCAGTTGATCAGTTGAATAAAATGAACCAGAAGAATATATCTGCTTGGAATATGAGGAGAATGATGAATATTATAAGAAAGGGAGCTTTGTTAACTCTTGATGAGAAGATATTGTCAGAGACGAATGAAGAATCATTGTTGCAGATTAGGAGTGAACGGCAGGCGAAAGAGGCAGCTGAGAAGATTTTCCAACGAGTTGTTCAACGTGGGTCAGACTGTATTTATCTGGAAGATGTAATGCGATTTATGAACAAAGAGGAAGCTCTAATGACCCTGAGTTTATTTGGAACAACCGCTGAAACCCAATGCATTGATGAGTCCTCTTTTAGAGAGTGGATGGTAAATGCATTTAAGCAGAGAAAAGTTCTTGTATTATCTCTCGGTGATGCAAATACAGCTGTTGATGATCTTCACAGCTTGCTGAACATCTTTGTAGCTATAATAATTTTGGTAATCTGGCTTATCATATTAGGAACTCCCGTTCGGCACTTTCTCATCTTCATAACTTCACAGCTTCTACTGCTGGTTTTTATTTTTGGTAACACATGTAAGACAGTCTTCGAAGCTATCATTTTCTTGTTTGTGATGCACCCATTTGACATTGGTGATCGCTGCGAAGTAGACGGTGTCCAGATGATTGTTGAAGAGATGAAGATATTAACAACTGTTTTTCTGAGATATGATAACCAGAAGATCACTTACCCCAACAGTGTGCTGGCTACTAAAGCCATTGGCAATTACTACCGCAGTCCAGACATGGGAGAGGGAGTTGATTTCTATGTCCACATCTCAACTCCATGGGAGAAAGTTGCCCTTTTGAAAGAACGGATAACAAGGTACGTAGAGAGCATGAGCGAGCTATGGCATCCAAATCCACAGATTGTGATGAAGGAAATTGAAGATCTGGAGAAGGTGAAGATGTCAGTTTGTGTTACACACAAAATGAACCACCAAGACATGAAAGAGAGATGGAACAGGAGAGCTGTGCTGGTTGAAGAGATTGTCACAATTTTGGAGTCAACTTGACAAAGAACATTAACATCTACAAATGTTGTCGGGGGGGAAGACTGTTTCAAACTTGCCCTTTGTCGGAATTAAGCTCACTCTAAACTGATAAGGGCCATAAGCTGCGTTTTGTGCATATTGTTGTTTTTTAGGAAATGATTTTAAGTGGATTTTCGTTTTCGAGGTGGAAATTGGTACATGCAGAAACGAACAAATTCATGATATTTTTTGGGTCATACTTTTACCCTTATATAAAATTAATTTCACATTAGTCTTTTCATGATTAGTAGATTAAGAGTTGATTAATGTGTTTACTAATAACAATGTTTTTGAAATAATATTAGTTTTGTATAAATGCTTTGAAAATAATATTAGTTTTTGTATAAAGTACTAATAGAGGTTTTTTTGTTTTTTTTATAAGTATAAATTGGAGAGAGGGACTAACAAATTTTCAAATAATATAAAACTTAATTAATACCATTTTTATGATTGAATCTATGATCGTAGAGTTAAAATAATTTACTTACTACCAATCAAGATAAATAATACATTACCAATATATTCAGTACCATCAACACATTCAGTACTAATATATAGGTTATAAGTTAAGTAAAAAATTTGTATTACTTGTTAAGTGTGATGCACCAAAGTTTAAAAAAAAAAAGTTATTGAAAATAATTCAATTAAAAAACTCAAAGCAGGTAATAAGAGATAAAATATCATTTTAGTTCTTTCGCTTTTCATTTTGTTTCTTGTAGGTCTATGTTTAGAAAATATACATTTT

Coding sequence (CDS)

ATGGCTTCGAAGGAAGAAGTATTTCTCAAGATCGACAGCTTCGGAAATGGGGGCGACAAGAAAATCCAGGGTTTACCAGAGAAATCGCCACAAACCATGAGACGAAGAGGCAAAGAGTTTCCAGAGCCGGAAACTCGCCGGCCCGGCGGCGGCGAGGTTCTCCGGTGCAGTTCCTACGCTTCATTTCGTGGAAGCTCATGGAGCTCGCCGATTAGCAAGAGATCTCGGCTGATGGATCCGCCTGAGGAAGATTCTTCTCAAAAGCTTGAAACCGCCGTGAAAGATGAAGGCAACAACAACAACAACGACGACGACAACAACAATCAGGATGATACAGAAGATATGCCTGAAGAATACGATCAACTGAAGCTAAGCCCCTTCTTTATTTTTCAGTTGGTTCTTTCTGTTTTTGTTATTGTGGGTTTGATTTGCAATCGATGGGTTTCGATCTTGAAGAGGGAGACGATTTGGGGTCTTCCGTTGTGGAAATGGGAACTGACTGTTCTAGCTGTGATCTGTGGGCATTTGGTTTCGATTTTGGCCGTTCGATTGATTGTGAAAATCATCGAGATGAATTTTTTGTTGAGGAAACGTGTTCTGTATTTCGTTTATGGGTTAAGGAATTCTGTTCAAATTTGCTTGTGGGTGTCTTTGATTTTGCTTGTTTGGCATTTCATTTTCAACCACAAGGTTCAGATTAAGACTCAGAGTAAGATTTTACCTTATGTTACCAAGATTTTCATCTGTTTTCTCGTGGGTGCTTCGATTTGGCTTCTGAAAACCCTTGCCATTAAAATTCTGGCTTCGTTTTTTCATGTTAATCACTACTTTGAAAGGCTTAAGGAGGCTTTGTTTAGTCAGTTTGTGATTGTGAGTCTCTCTGGTTTGCCATTGTTCAAGCGGCCGAATACTAAAGATGTGATGATGGGAGACCAGAATGCTGATGGAAGAGAAGAAAGTCCCAAAAGTGGGAGAGTTATATGCAGTGGGAGACTTGTAGATTGCAATCGATCGAGACCGTTGAAGCCCGATTCGGTTAAAGAAGATGAGGAAATCCCAGTTGATCAGTTGAATAAAATGAACCAGAAGAATATATCTGCTTGGAATATGAGGAGAATGATGAATATTATAAGAAAGGGAGCTTTGTTAACTCTTGATGAGAAGATATTGTCAGAGACGAATGAAGAATCATTGTTGCAGATTAGGAGTGAACGGCAGGCGAAAGAGGCAGCTGAGAAGATTTTCCAACGAGTTGTTCAACGTGGGTCAGACTGTATTTATCTGGAAGATGTAATGCGATTTATGAACAAAGAGGAAGCTCTAATGACCCTGAGTTTATTTGGAACAACCGCTGAAACCCAATGCATTGATGAGTCCTCTTTTAGAGAGTGGATGGTAAATGCATTTAAGCAGAGAAAAGTTCTTGTATTATCTCTCGGTGATGCAAATACAGCTGTTGATGATCTTCACAGCTTGCTGAACATCTTTGTAGCTATAATAATTTTGGTAATCTGGCTTATCATATTAGGAACTCCCGTTCGGCACTTTCTCATCTTCATAACTTCACAGCTTCTACTGCTGGTTTTTATTTTTGGTAACACATGTAAGACAGTCTTCGAAGCTATCATTTTCTTGTTTGTGATGCACCCATTTGACATTGGTGATCGCTGCGAAGTAGACGGTGTCCAGATGATTGTTGAAGAGATGAAGATATTAACAACTGTTTTTCTGAGATATGATAACCAGAAGATCACTTACCCCAACAGTGTGCTGGCTACTAAAGCCATTGGCAATTACTACCGCAGTCCAGACATGGGAGAGGGAGTTGATTTCTATGTCCACATCTCAACTCCATGGGAGAAAGTTGCCCTTTTGAAAGAACGGATAACAAGGTACGTAGAGAGCATGAGCGAGCTATGGCATCCAAATCCACAGATTGTGATGAAGGAAATTGAAGATCTGGAGAAGGTGAAGATGTCAGTTTGTGTTACACACAAAATGAACCACCAAGACATGAAAGAGAGATGGAACAGGAGAGCTGTGCTGGTTGAAGAGATTGTCACAATTTTGGAGTCAACTTGA

Protein sequence

MASKEEVFLKIDSFGNGGDKKIQGLPEKSPQTMRRRGKEFPEPETRRPGGGEVLRCSSYASFRGSSWSSPISKRSRLMDPPEEDSSQKLETAVKDEGNNNNNDDDNNNQDDTEDMPEEYDQLKLSPFFIFQLVLSVFVIVGLICNRWVSILKRETIWGLPLWKWELTVLAVICGHLVSILAVRLIVKIIEMNFLLRKRVLYFVYGLRNSVQICLWVSLILLVWHFIFNHKVQIKTQSKILPYVTKIFICFLVGASIWLLKTLAIKILASFFHVNHYFERLKEALFSQFVIVSLSGLPLFKRPNTKDVMMGDQNADGREESPKSGRVICSGRLVDCNRSRPLKPDSVKEDEEIPVDQLNKMNQKNISAWNMRRMMNIIRKGALLTLDEKILSETNEESLLQIRSERQAKEAAEKIFQRVVQRGSDCIYLEDVMRFMNKEEALMTLSLFGTTAETQCIDESSFREWMVNAFKQRKVLVLSLGDANTAVDDLHSLLNIFVAIIILVIWLIILGTPVRHFLIFITSQLLLLVFIFGNTCKTVFEAIIFLFVMHPFDIGDRCEVDGVQMIVEEMKILTTVFLRYDNQKITYPNSVLATKAIGNYYRSPDMGEGVDFYVHISTPWEKVALLKERITRYVESMSELWHPNPQIVMKEIEDLEKVKMSVCVTHKMNHQDMKERWNRRAVLVEEIVTILEST
Homology
BLAST of Sed0028199 vs. NCBI nr
Match: XP_022936287.1 (mechanosensitive ion channel protein 8-like [Cucurbita moschata])

HSP 1 Score: 1109.7 bits (2869), Expect = 0.0e+00
Identity = 574/701 (81.88%), Postives = 634/701 (90.44%), Query Frame = 0

Query: 1   MASKEEVFLKIDSFGNGG----DKKIQGLPEKSPQTMRRRGKEFPEPETRRP----GGGE 60
           MA KE+V LK++SFGNG     +KK Q LPE+S QTMRRRGKEF EPE R+P     GGE
Sbjct: 1   MAPKEDVILKVNSFGNGDKELVEKKFQCLPERSLQTMRRRGKEFSEPENRQPSSGSSGGE 60

Query: 61  VLRCSSYASFRGSSWSSPISKRSRLMDPPEEDSSQKLETAVKDEGNNNNNDDDNNNQDDT 120
           VLRCSS  SF GSSWSSPISK+SRLMDPPE+DSSQKLETAVKDEGNN      NNNQD T
Sbjct: 61  VLRCSSNYSFSGSSWSSPISKKSRLMDPPEDDSSQKLETAVKDEGNN------NNNQDGT 120

Query: 121 EDMPEEYDQLKLSPFFIFQLVLSVFVIVGLICNRWVSILKRETIWGLPLWKWELTVLAVI 180
           EDMPEEY+QLKLSP   FQLV+ V +IV LICNRW+SILKRET WGLPLWKWELTVLAVI
Sbjct: 121 EDMPEEYNQLKLSPLLGFQLVVFVLIIVSLICNRWISILKRETFWGLPLWKWELTVLAVI 180

Query: 181 CGHLVSILAVRLIVKIIEMNFLLRKRVLYFVYGLRNSVQICLWVSLILLVWHFIFNHKVQ 240
           CGH+VS +AV L+VKIIEMNFLLRKRVLYFVYGLR +V+ CLW+SLILLVWHFIFN KVQ
Sbjct: 181 CGHMVSSMAVGLMVKIIEMNFLLRKRVLYFVYGLRKAVKNCLWMSLILLVWHFIFNDKVQ 240

Query: 241 IKTQSKILPYVTKIFICFLVGASIWLLKTLAIKILASFFHVNHYFERLKEALFSQFVIVS 300
            KTQSKILPYVTKI ICFLV ASIWLLKTL IKILASFFHVN+YFERLKEALFSQ+VIV+
Sbjct: 241 RKTQSKILPYVTKILICFLVAASIWLLKTLVIKILASFFHVNNYFERLKEALFSQYVIVT 300

Query: 301 LSGLPLFKRPNTKDVMMGDQNADGREESPKSGRVICSGRLVDCNRSRPLKPDSVKEDEEI 360
           LSGLPLF+RPN++D+M  +Q  DG+ ES KSGRV+CSGRLV+CN  R LK +S ++DEEI
Sbjct: 301 LSGLPLFERPNSEDMMKDEQIGDGKAESSKSGRVVCSGRLVNCNGWRQLKVESKRKDEEI 360

Query: 361 PVDQLNKMNQKNISAWNMRRMMNIIRKGALLTLDEKILSETNEESLLQIRSERQAKEAAE 420
           PVD+LNK+NQKNISAWNMRRMMNI+R GALLTLDEKILSET E+SLLQIR+ERQAKEAA 
Sbjct: 361 PVDELNKLNQKNISAWNMRRMMNIVRNGALLTLDEKILSETEEQSLLQIRNERQAKEAAG 420

Query: 421 KIFQRVVQRGSDCIYLEDVMRFMNKEEALMTLSLFGTTAETQCIDESSFREWMVNAFKQR 480
           +IF+RV QRGSDCIY+EDVMRFMNKEEAL+T+SLFGTTAETQCIDESSFREWMVNAFKQR
Sbjct: 421 RIFRRVAQRGSDCIYIEDVMRFMNKEEALVTMSLFGTTAETQCIDESSFREWMVNAFKQR 480

Query: 481 KVLVLSLGDANTAVDDLHSLLNIFVAIIILVIWLIILGTPVRHFLIFITSQLLLLVFIFG 540
           KVLVLSL DANTAVDDLH+LLN+FVA+ ILVIWLII GTPV HFLIFITSQLLLL FIFG
Sbjct: 481 KVLVLSLNDANTAVDDLHNLLNVFVALTILVIWLIIFGTPVLHFLIFITSQLLLLAFIFG 540

Query: 541 NTCKTVFEAIIFLFVMHPFDIGDRCEVDGVQMIVEEMKILTTVFLRYDNQKITYPNSVLA 600
           NTCKTVFEAIIFLFVMHPFD+GDRCEVDGV+M+VEEMKILTTVFLRYDNQ+I+YPNSVLA
Sbjct: 541 NTCKTVFEAIIFLFVMHPFDVGDRCEVDGVEMVVEEMKILTTVFLRYDNQRISYPNSVLA 600

Query: 601 TKAIGNYYRSPDMGEGVDFYVHISTPWEKVALLKERITRYVESMSELWHPNPQIVMKEIE 660
           TKAIGNYYRSPDMGEGVDFY+HISTPWEKVALLKERITRYVESM E WHP+PQIVMK++E
Sbjct: 601 TKAIGNYYRSPDMGEGVDFYIHISTPWEKVALLKERITRYVESMGEQWHPDPQIVMKDVE 660

Query: 661 DLEKVKMSVCVTHKMNHQDMKERWNRRAVLVEEIVTILEST 694
           DLEKV+MSV +THK+NHQ+MKERWNRRA LV+EIVTIL+ST
Sbjct: 661 DLEKVRMSVGLTHKLNHQNMKERWNRRAALVQEIVTILDST 695

BLAST of Sed0028199 vs. NCBI nr
Match: XP_022975440.1 (mechanosensitive ion channel protein 8-like [Cucurbita maxima])

HSP 1 Score: 1109.4 bits (2868), Expect = 0.0e+00
Identity = 573/701 (81.74%), Postives = 636/701 (90.73%), Query Frame = 0

Query: 1   MASKEEVFLKIDSFGNGG----DKKIQGLPEKSPQTMRRRGKEFPEPETRR----PGGGE 60
           MA KE+V LK++SFG+G     +KK Q LPE+S QTMRRRGKEF EPE R+      GGE
Sbjct: 1   MAPKEDVILKVNSFGHGDKELVEKKFQCLPERSLQTMRRRGKEFSEPENRQLSSGSSGGE 60

Query: 61  VLRCSSYASFRGSSWSSPISKRSRLMDPPEEDSSQKLETAVKDEGNNNNNDDDNNNQDDT 120
           VLRCSS +SF GSSWSSPISK+SRLMDPPE+DSSQKLETAVKDEGNN      NNNQD T
Sbjct: 61  VLRCSSNSSFPGSSWSSPISKKSRLMDPPEDDSSQKLETAVKDEGNN------NNNQDGT 120

Query: 121 EDMPEEYDQLKLSPFFIFQLVLSVFVIVGLICNRWVSILKRETIWGLPLWKWELTVLAVI 180
           EDMPEEY+QLKLSP   FQLV+ V +IV LICNRW+SILKRET WGLPLWKWELTVLAVI
Sbjct: 121 EDMPEEYNQLKLSPLLGFQLVVFVLIIVSLICNRWISILKRETFWGLPLWKWELTVLAVI 180

Query: 181 CGHLVSILAVRLIVKIIEMNFLLRKRVLYFVYGLRNSVQICLWVSLILLVWHFIFNHKVQ 240
           CGH+VS +AV L+VKIIEMNFLLRKRVLYFVYGLR +V+ CLW+SLILLVWHFIFN KVQ
Sbjct: 181 CGHMVSSMAVGLMVKIIEMNFLLRKRVLYFVYGLRKAVKNCLWMSLILLVWHFIFNDKVQ 240

Query: 241 IKTQSKILPYVTKIFICFLVGASIWLLKTLAIKILASFFHVNHYFERLKEALFSQFVIVS 300
            KTQSKILPYVTKI ICFLV A+IWLLKTL IKILASFFHVN+YFERLKEALFSQ+VIV+
Sbjct: 241 RKTQSKILPYVTKILICFLVAATIWLLKTLVIKILASFFHVNNYFERLKEALFSQYVIVT 300

Query: 301 LSGLPLFKRPNTKDVMMGDQNADGREESPKSGRVICSGRLVDCNRSRPLKPDSVKEDEEI 360
           LSGLPLF+RPN++D+M  +Q ADG+ ES KSGRV+CSGRLV+CN SR LK +S ++DEEI
Sbjct: 301 LSGLPLFERPNSEDMMKDEQIADGKAESSKSGRVVCSGRLVNCNGSRQLKVESKRKDEEI 360

Query: 361 PVDQLNKMNQKNISAWNMRRMMNIIRKGALLTLDEKILSETNEESLLQIRSERQAKEAAE 420
           PVD+LNK+NQKNISAWNMRRMMNI+R GALLTLDEKILSET E+SLLQIRSERQAKEAA 
Sbjct: 361 PVDELNKLNQKNISAWNMRRMMNIVRNGALLTLDEKILSETKEQSLLQIRSERQAKEAAG 420

Query: 421 KIFQRVVQRGSDCIYLEDVMRFMNKEEALMTLSLFGTTAETQCIDESSFREWMVNAFKQR 480
           +IF+RV QRGSDCIY+EDVMRFMNKEEAL+T+SLFGTTAETQCIDESSFREWMVNAFKQR
Sbjct: 421 RIFRRVAQRGSDCIYIEDVMRFMNKEEALVTMSLFGTTAETQCIDESSFREWMVNAFKQR 480

Query: 481 KVLVLSLGDANTAVDDLHSLLNIFVAIIILVIWLIILGTPVRHFLIFITSQLLLLVFIFG 540
           KVLVLSL DANTAVDDLH+LLN+FVA+ ILVIWLI+ GTPV HFLIFITSQLLLL FIFG
Sbjct: 481 KVLVLSLNDANTAVDDLHNLLNVFVALTILVIWLIVFGTPVLHFLIFITSQLLLLAFIFG 540

Query: 541 NTCKTVFEAIIFLFVMHPFDIGDRCEVDGVQMIVEEMKILTTVFLRYDNQKITYPNSVLA 600
           NTCKTVFEAIIFLFVMHPFD+GDRCEVDGV+M+VEEMKILTTVFLRYDNQ+I+YPNSVLA
Sbjct: 541 NTCKTVFEAIIFLFVMHPFDVGDRCEVDGVEMVVEEMKILTTVFLRYDNQRISYPNSVLA 600

Query: 601 TKAIGNYYRSPDMGEGVDFYVHISTPWEKVALLKERITRYVESMSELWHPNPQIVMKEIE 660
           TKAIGNYYRSPDMGEGVDFY+HISTPWEKVALLKERITRYVESM E WHP+PQIVMK++E
Sbjct: 601 TKAIGNYYRSPDMGEGVDFYIHISTPWEKVALLKERITRYVESMGEQWHPDPQIVMKDVE 660

Query: 661 DLEKVKMSVCVTHKMNHQDMKERWNRRAVLVEEIVTILEST 694
           DLEKV+MSV +THK+NHQ+MKERWNRRA LV+EIVTIL+ST
Sbjct: 661 DLEKVRMSVGLTHKLNHQNMKERWNRRAALVQEIVTILDST 695

BLAST of Sed0028199 vs. NCBI nr
Match: XP_023535846.1 (mechanosensitive ion channel protein 8-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023535847.1 mechanosensitive ion channel protein 8-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1104.0 bits (2854), Expect = 0.0e+00
Identity = 571/701 (81.46%), Postives = 633/701 (90.30%), Query Frame = 0

Query: 1   MASKEEVFLKIDSFGNGG----DKKIQGLPEKSPQTMRRRGKEFPEPETRRP----GGGE 60
           MA KE+V LK++SF NG     +KK Q LPE+S QTMRRRGKEF EP+ R+P     GGE
Sbjct: 1   MAPKEDVILKVNSFRNGDKELVEKKFQCLPERSLQTMRRRGKEFSEPDNRQPSSGSSGGE 60

Query: 61  VLRCSSYASFRGSSWSSPISKRSRLMDPPEEDSSQKLETAVKDEGNNNNNDDDNNNQDDT 120
           VLRCSS  SF GSSWSSPISK+SRLMDPPE+DSSQKLETAVKDEGNN      N+NQD T
Sbjct: 61  VLRCSSNYSFSGSSWSSPISKKSRLMDPPEDDSSQKLETAVKDEGNN------NDNQDGT 120

Query: 121 EDMPEEYDQLKLSPFFIFQLVLSVFVIVGLICNRWVSILKRETIWGLPLWKWELTVLAVI 180
           EDMPEEY+QLKLSP   FQLV+ V +IV LICNRW+SILKRET WGLPLWKWELTVLAVI
Sbjct: 121 EDMPEEYNQLKLSPLLGFQLVVFVLIIVSLICNRWISILKRETFWGLPLWKWELTVLAVI 180

Query: 181 CGHLVSILAVRLIVKIIEMNFLLRKRVLYFVYGLRNSVQICLWVSLILLVWHFIFNHKVQ 240
           CGH+VS +AV L+VKIIEMNFLLRKRVLYFVYGLR +V+ CLW SLILLVWHFIFN KVQ
Sbjct: 181 CGHMVSSMAVGLMVKIIEMNFLLRKRVLYFVYGLRKAVKNCLWTSLILLVWHFIFNDKVQ 240

Query: 241 IKTQSKILPYVTKIFICFLVGASIWLLKTLAIKILASFFHVNHYFERLKEALFSQFVIVS 300
            KTQSKILPYVTKI ICFLV ASIWLLKTL IKILASFFHVN+YFERLKEALFSQ+VIV+
Sbjct: 241 RKTQSKILPYVTKILICFLVAASIWLLKTLVIKILASFFHVNNYFERLKEALFSQYVIVT 300

Query: 301 LSGLPLFKRPNTKDVMMGDQNADGREESPKSGRVICSGRLVDCNRSRPLKPDSVKEDEEI 360
           LSGLPLF+RPN++D+M  +Q  DG+ ES KSGRV+CSGRLV+CN SR LK +S ++DEEI
Sbjct: 301 LSGLPLFERPNSEDMMKDEQIDDGKAESSKSGRVVCSGRLVNCNGSRQLKVESKRKDEEI 360

Query: 361 PVDQLNKMNQKNISAWNMRRMMNIIRKGALLTLDEKILSETNEESLLQIRSERQAKEAAE 420
           PVD+LNK+NQKNISAWNMRRMMNI+R GALLTLDEKILSET E+SLLQIR+ERQAKEAA 
Sbjct: 361 PVDELNKLNQKNISAWNMRRMMNIVRNGALLTLDEKILSETEEQSLLQIRNERQAKEAAG 420

Query: 421 KIFQRVVQRGSDCIYLEDVMRFMNKEEALMTLSLFGTTAETQCIDESSFREWMVNAFKQR 480
           +IF+RV QRGSDCIY+EDVMRFMNKEEAL+T+SLFGTTAETQCIDESSFREWMVNAFKQR
Sbjct: 421 RIFRRVAQRGSDCIYIEDVMRFMNKEEALVTMSLFGTTAETQCIDESSFREWMVNAFKQR 480

Query: 481 KVLVLSLGDANTAVDDLHSLLNIFVAIIILVIWLIILGTPVRHFLIFITSQLLLLVFIFG 540
           KVLVLSL DANTAVDDLH+LLN+FVA+ ILVIWLII GTPV HFLIFITSQLLLL FIFG
Sbjct: 481 KVLVLSLNDANTAVDDLHNLLNVFVALTILVIWLIIFGTPVLHFLIFITSQLLLLAFIFG 540

Query: 541 NTCKTVFEAIIFLFVMHPFDIGDRCEVDGVQMIVEEMKILTTVFLRYDNQKITYPNSVLA 600
           NTCKTVFEAIIFLFVMHPFD+GDRCEVDGV+M+VEEMKILTTVFLRYDNQ+I+YPNSVLA
Sbjct: 541 NTCKTVFEAIIFLFVMHPFDVGDRCEVDGVEMVVEEMKILTTVFLRYDNQRISYPNSVLA 600

Query: 601 TKAIGNYYRSPDMGEGVDFYVHISTPWEKVALLKERITRYVESMSELWHPNPQIVMKEIE 660
           TKAIGNYYRSPDMGEGVDFY+HISTPWEKVALLKERITRYVESM E WHP+PQIVMK++E
Sbjct: 601 TKAIGNYYRSPDMGEGVDFYIHISTPWEKVALLKERITRYVESMGEQWHPDPQIVMKDVE 660

Query: 661 DLEKVKMSVCVTHKMNHQDMKERWNRRAVLVEEIVTILEST 694
           DLEKV+MSV +TH++NHQ+MKERWNRRA LV+EIVTIL+ST
Sbjct: 661 DLEKVRMSVGLTHRLNHQNMKERWNRRAALVQEIVTILDST 695

BLAST of Sed0028199 vs. NCBI nr
Match: XP_022140870.1 (mechanosensitive ion channel protein 6-like isoform X1 [Momordica charantia])

HSP 1 Score: 1065.1 bits (2753), Expect = 2.6e-307
Identity = 557/698 (79.80%), Postives = 615/698 (88.11%), Query Frame = 0

Query: 1   MASKEEVFLKIDSFGNGGDKK-----IQGLPEKSPQTMRRRGKEFPEPETRRP----GGG 60
           MA +E+V LKIDSFGNG DK+      Q LPE+S QTMRRRGKE  + E RRP    GGG
Sbjct: 1   MAPREDVLLKIDSFGNGADKESEEKNFQCLPERSAQTMRRRGKESSDSENRRPSSASGGG 60

Query: 61  EVLRCSSYASFRGSSWSSPISKRSRLMDPPEEDSSQKLETAVKDEGNNNNNDDDNNNQDD 120
           EVLRCSS ASFRG SWSSPISK+SRLMDPPEEDSSQKLETAV +EG          NQD+
Sbjct: 61  EVLRCSSNASFRGCSWSSPISKKSRLMDPPEEDSSQKLETAVSEEG---------KNQDE 120

Query: 121 TEDMPEEYDQLKLSPFFIFQLVLSVFVIVGLICNRWVSILKRETIWGLPLWKWELTVLAV 180
           TEDMPEEY+QLKLSPF + Q V+ V VIVGLICNRWV ILKR++IW LPLWKWELTVLA+
Sbjct: 121 TEDMPEEYNQLKLSPFSVLQWVIFVIVIVGLICNRWVPILKRKSIWDLPLWKWELTVLAL 180

Query: 181 ICGHLVSILAVRLIVKIIEMNFLLRKRVLYFVYGLRNSVQICLWVSLILLVWHFIFNHKV 240
           ICG L+S  AV LIVKIIE NFLLRKRVLYFVYGLR +V+ CLW+SL+LLVWHFIFN KV
Sbjct: 181 ICGRLISTWAVGLIVKIIERNFLLRKRVLYFVYGLRRAVRNCLWLSLLLLVWHFIFNEKV 240

Query: 241 QIKTQSKILPYVTKIFICFLVGASIWLLKTLAIKILASFFHVNHYFERLKEALFSQFVIV 300
           Q  T+SKILPY+TKI ICFLVGASIWLLKTLA+KILASFFHVN+YFERLKEALFSQ+VIV
Sbjct: 241 QRTTRSKILPYITKILICFLVGASIWLLKTLAVKILASFFHVNNYFERLKEALFSQYVIV 300

Query: 301 SLSGLPLFKRPNTKDVMMGDQNADGREESPKSGRVICSGRLVDCNRSRPLKPDSVKEDEE 360
           +LSG PLF+R NT+D++  +QNADGREESPKSGR+ICSGRLV+C+ SR LKP+S ++DEE
Sbjct: 301 TLSGSPLFERSNTEDMVKDNQNADGREESPKSGRIICSGRLVNCDGSRQLKPESSRQDEE 360

Query: 361 IPVDQLNKMNQKNISAWNMRRMMNIIRKGALLTLDEKILSETNEESLLQIRSERQAKEAA 420
           IPVDQLNK+NQ NISAWNMRRMMNI+R GALLTLDEKILSET EE LLQIRSERQAKEAA
Sbjct: 361 IPVDQLNKLNQHNISAWNMRRMMNIVRNGALLTLDEKILSETKEEYLLQIRSERQAKEAA 420

Query: 421 EKIFQRVVQRGSDCIYLEDVMRFMNKEEALMTLSLFGTTAETQCIDESSFREWMVNAFKQ 480
             IF+RV +RGS+ I L+DVMRFMNKEEALMT+SLFG  AETQCIDE SFREWMVNAF Q
Sbjct: 421 GNIFRRVSRRGSEFINLDDVMRFMNKEEALMTMSLFGPDAETQCIDEFSFREWMVNAFNQ 480

Query: 481 RKVLVLSLGDANTAVDDLHSLLNIFVAIIILVIWLIILGTPVRHFLIFITSQLLLLVFIF 540
           RKVLVLSL DANTAVD LH+LLNIFVA+ ILVIWLIILGTP+ HFLIFI+SQLLLLVFIF
Sbjct: 481 RKVLVLSLNDANTAVDGLHNLLNIFVAVTILVIWLIILGTPILHFLIFISSQLLLLVFIF 540

Query: 541 GNTCKTVFEAIIFLFVMHPFDIGDRCEVDGVQMIVEEMKILTTVFLRYDNQKITYPNSVL 600
           GNTCKTVFEAIIFLFVMHPFD+GDRCEVDGVQM+VEEM+ILTTVFLRYDNQKITYPNSVL
Sbjct: 541 GNTCKTVFEAIIFLFVMHPFDVGDRCEVDGVQMVVEEMRILTTVFLRYDNQKITYPNSVL 600

Query: 601 ATKAIGNYYRSPDMGEGVDFYVHISTPWEKVALLKERITRYVESMSELWHPNPQIVMKEI 660
           ATKAIGNYYRSPDMGEGVDFYVHISTP +KV+L+KE+ITRY+ES SE WHP P + MK++
Sbjct: 601 ATKAIGNYYRSPDMGEGVDFYVHISTPSKKVSLIKEQITRYIESKSEEWHPAPVVTMKDV 660

Query: 661 EDLEKVKMSVCVTHKMNHQDMKERWNRRAVLVEEIVTI 690
           EDLEKVKMSVCVTHKMNHQDM ERWNRRA LVEEIV I
Sbjct: 661 EDLEKVKMSVCVTHKMNHQDMGERWNRRAALVEEIVKI 689

BLAST of Sed0028199 vs. NCBI nr
Match: XP_023535848.1 (mechanosensitive ion channel protein 8-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1031.6 bits (2666), Expect = 3.2e-297
Identity = 540/701 (77.03%), Postives = 602/701 (85.88%), Query Frame = 0

Query: 1   MASKEEVFLKIDSFGNGG----DKKIQGLPEKSPQTMRRRGKEFPEPETRRP----GGGE 60
           MA KE+V LK++SF NG     +KK Q LPE+S QTMRRRGKEF EP+ R+P     GGE
Sbjct: 1   MAPKEDVILKVNSFRNGDKELVEKKFQCLPERSLQTMRRRGKEFSEPDNRQPSSGSSGGE 60

Query: 61  VLRCSSYASFRGSSWSSPISKRSRLMDPPEEDSSQKLETAVKDEGNNNNNDDDNNNQDDT 120
           VLRCSS  SF GSSWSSPISK+SRLMDPPE+DSSQKLETAVKDEGNN      N+NQD T
Sbjct: 61  VLRCSSNYSFSGSSWSSPISKKSRLMDPPEDDSSQKLETAVKDEGNN------NDNQDGT 120

Query: 121 EDMPEEYDQLKLSPFFIFQLVLSVFVIVGLICNRWVSILKRETIWGLPLWKWELTVLAVI 180
           EDMPEEY+QLKLSP   FQLV+ V +IV LICNRW+SILKRET WGLPLWKWELTVLAVI
Sbjct: 121 EDMPEEYNQLKLSPLLGFQLVVFVLIIVSLICNRWISILKRETFWGLPLWKWELTVLAVI 180

Query: 181 CGHLVSILAVRLIVKIIEMNFLLRKRVLYFVYGLRNSVQICLWVSLILLVWHFIFNHKVQ 240
           CGH+VS +AV L+VKIIEMNFLLRKRVLYFVYGLR +V+ CLW SLILLVWHFIFN KVQ
Sbjct: 181 CGHMVSSMAVGLMVKIIEMNFLLRKRVLYFVYGLRKAVKNCLWTSLILLVWHFIFNDKVQ 240

Query: 241 IKTQSKILPYVTKIFICFLVGASIWLLKTLAIKILASFFHVNHYFERLKEALFSQFVIVS 300
            KTQSKILPYVTKI ICFLV ASIWLLKTL IKILASFFHVN+YFERLKEALFSQ+VIV+
Sbjct: 241 RKTQSKILPYVTKILICFLVAASIWLLKTLVIKILASFFHVNNYFERLKEALFSQYVIVT 300

Query: 301 LSGLPLFKRPNTKDVMMGDQNADGREESPKSGRVICSGRLVDCNRSRPLKPDSVKEDEEI 360
           LSGLPLF+RPN++D+M  +Q  DG+ ES KSGRV+CSGRLV+CN SR LK +S ++DEEI
Sbjct: 301 LSGLPLFERPNSEDMMKDEQIDDGKAESSKSGRVVCSGRLVNCNGSRQLKVESKRKDEEI 360

Query: 361 PVDQLNKMNQKNISAWNMRRMMNIIRKGALLTLDEKILSETNEESLLQIRSERQAKEAAE 420
           PVD+LNK+NQKNISAWNMRRMMNI+R GALLTLDEKILSET E+SLLQIR+ERQAKEAA 
Sbjct: 361 PVDELNKLNQKNISAWNMRRMMNIVRNGALLTLDEKILSETEEQSLLQIRNERQAKEAAG 420

Query: 421 KIFQRVVQRGSDCIYLEDVMRFMNKEEALMTLSLFGTTAETQCIDESSFREWMVNAFKQR 480
           +IF+RV QRGSDCIY+EDVMRFMNKEEAL+T+SLFGTTAETQCIDESSFREWMVNAFKQR
Sbjct: 421 RIFRRVAQRGSDCIYIEDVMRFMNKEEALVTMSLFGTTAETQCIDESSFREWMVNAFKQR 480

Query: 481 KVLVLSLGDANTAVDDLHSLLNIFVAIIILVIWLIILGTPVRHFLIFITSQLLLLVFIFG 540
           KVLVLSL DANTAVDDLH+LLN+FVA+ IL                              
Sbjct: 481 KVLVLSLNDANTAVDDLHNLLNVFVALTIL------------------------------ 540

Query: 541 NTCKTVFEAIIFLFVMHPFDIGDRCEVDGVQMIVEEMKILTTVFLRYDNQKITYPNSVLA 600
               TVFEAIIFLFVMHPFD+GDRCEVDGV+M+VEEMKILTTVFLRYDNQ+I+YPNSVLA
Sbjct: 541 ----TVFEAIIFLFVMHPFDVGDRCEVDGVEMVVEEMKILTTVFLRYDNQRISYPNSVLA 600

Query: 601 TKAIGNYYRSPDMGEGVDFYVHISTPWEKVALLKERITRYVESMSELWHPNPQIVMKEIE 660
           TKAIGNYYRSPDMGEGVDFY+HISTPWEKVALLKERITRYVESM E WHP+PQIVMK++E
Sbjct: 601 TKAIGNYYRSPDMGEGVDFYIHISTPWEKVALLKERITRYVESMGEQWHPDPQIVMKDVE 660

Query: 661 DLEKVKMSVCVTHKMNHQDMKERWNRRAVLVEEIVTILEST 694
           DLEKV+MSV +TH++NHQ+MKERWNRRA LV+EIVTIL+ST
Sbjct: 661 DLEKVRMSVGLTHRLNHQNMKERWNRRAALVQEIVTILDST 661

BLAST of Sed0028199 vs. ExPASy Swiss-Prot
Match: F4IME2 (Mechanosensitive ion channel protein 8 OS=Arabidopsis thaliana OX=3702 GN=MSL8 PE=2 SV=2)

HSP 1 Score: 587.8 bits (1514), Expect = 1.6e-166
Identity = 324/661 (49.02%), Postives = 457/661 (69.14%), Query Frame = 0

Query: 52  EVLRCSSYASF-RGSSWSSPISKRSRLMDPPEEDSSQKLETAVKDEGNNNNNDDDNNNQD 111
           EV+RC+S  SF R S   S +  RSRL DPP E+ +    +  +     +    D + +D
Sbjct: 222 EVVRCTSNMSFQRKSELISRVKTRSRLQDPPREEETP--YSGWRSGQLKSGLLADIDEED 281

Query: 112 D---TEDMPEEYDQLKLSPFFIFQLVLSVFVIVGLICNRWVSILKRETIWGLPLWKWELT 171
           D    ED+P+EY + KL    + Q +  V +I  L C+  +   K+  +W L LWKWE+ 
Sbjct: 282 DPLAEEDVPDEYKRGKLDAITLLQWLSLVAIIAALACSLSIQSWKKVRVWNLHLWKWEVF 341

Query: 172 VLAVICGHLVSILAVRLIVKIIEMNFLLRKRVLYFVYGLRNSVQICLWVSLILLVWHFIF 231
           +L +ICG LVS   +R++V  IE NFLLRKRVLYFVYG+R +VQ CLW+ L+LL WHF+F
Sbjct: 342 LLVLICGRLVSGWGIRIVVFFIERNFLLRKRVLYFVYGVRRAVQNCLWLGLVLLAWHFLF 401

Query: 232 NHKVQIKTQSKILPYVTKIFICFLVGASIWLLKTLAIKILASFFHVNHYFERLKEALFSQ 291
           + KVQ +T+S+ LPYVTKI +CFL+   +WL+KTL +K+LAS FHV+ YF+R++EALF+Q
Sbjct: 402 DKKVQRETRSRFLPYVTKILVCFLLSTILWLIKTLVVKVLASSFHVSTYFDRIQEALFNQ 461

Query: 292 FVIVSLSGLPLF-------KRPNTKDVMMGDQNADGREESP----------KSGRVICSG 351
           +VI +LSG P+        +    +D +   QNA G    P          KSGRV+   
Sbjct: 462 YVIETLSGPPMIEMSRIEEEEERAQDEIFKMQNA-GANLPPDLCAAAFPPGKSGRVMNP- 521

Query: 352 RLVDCNRSRPLKPDSVKEDEEIPVDQLNKMNQKNISAWNMRRMMNIIRKGALLTLDEKIL 411
                 +  P+ P S   D  I ++ L++MN KNISAWNM+R+M I+R  +L TLDE++L
Sbjct: 522 ------KLSPIIPKST-TDNGISMEHLHRMNHKNISAWNMKRLMKIVRNVSLTTLDEQML 581

Query: 412 SETNE-ESLLQIRSERQAKEAAEKIFQRVVQRGSDCIYLEDVMRFMNKEEALMTLSLFGT 471
             T E ES  QIRSE++AK AA KIF+ V QRG+  IYLED+MRF+ ++EA+ T+ LF  
Sbjct: 582 ESTYEDESTRQIRSEKEAKAAARKIFKNVEQRGAKYIYLEDLMRFLREDEAMKTMGLFEG 641

Query: 472 TAETQCIDESSFREWMVNAFKQRKVLVLSLGDANTAVDDLHSLLNIFVAIIILVIWLIIL 531
             E + I +S+ + W+VNAF++R+ L L+L D  TAV+ LH ++NI  AI+I+VIWL++L
Sbjct: 642 APENKRISKSALKNWLVNAFRERRALALTLNDTKTAVNKLHHMINIVTAIVIVVIWLVLL 701

Query: 532 GTPVRHFLIFITSQLLLLVFIFGNTCKTVFEAIIFLFVMHPFDIGDRCEVDGVQMIVEEM 591
                  L+F++SQ++LL FIFGNT KTVFE+IIFLF++HP+D+GDRCE+D VQ++VEEM
Sbjct: 702 EIASSKVLLFVSSQVVLLAFIFGNTVKTVFESIIFLFIVHPYDVGDRCEIDSVQLVVEEM 761

Query: 592 KILTTVFLRYDNQKITYPNSVLATKAIGNYYRSPDMGEGVDFYVHISTPWEKVALLKERI 651
            ILTTVFLRYDN KI YPNS+L  K+I NYYRSPDMG+ ++F VHI+TP EK++++K+RI
Sbjct: 762 NILTTVFLRYDNLKIMYPNSLLWQKSINNYYRSPDMGDAIEFCVHITTPLEKISVIKQRI 821

Query: 652 TRYVESMSELWHPNPQIVMKEIEDLEKVKMSVCVTHKMNHQDMKERWNRRAVLVEEIVTI 691
           + Y+++  E W+P  +I++K++EDL  V++++   H++NHQDM ERW RRAVLVEE++ I
Sbjct: 822 SNYIDNKPEYWYPQAKIIVKDLEDLHIVRLAIWPCHRINHQDMAERWTRRAVLVEEVIKI 871

BLAST of Sed0028199 vs. ExPASy Swiss-Prot
Match: Q9SYM1 (Mechanosensitive ion channel protein 6 OS=Arabidopsis thaliana OX=3702 GN=MSL6 PE=1 SV=1)

HSP 1 Score: 574.7 bits (1480), Expect = 1.4e-162
Identity = 324/702 (46.15%), Postives = 456/702 (64.96%), Query Frame = 0

Query: 28  KSPQTMRRR---GKEFPEPETRRPGGGEVLRCSSYAS--FRGSSWSSPISKRSRLMDPPE 87
           +SP+   +R   G+      T     GEV++CS   +   R SS    +  RSRL DPP 
Sbjct: 123 ESPRVSTKRDPVGRRDSRSNTNNNDDGEVVKCSGNNAPIQRSSSTLLKMRTRSRLSDPPT 182

Query: 88  ED--------------SSQKLETAVKDEGNNNNNDDDNNNQDDTEDMPEEYDQLKLSPFF 147
                            S ++++    +      +++ ++    ED+PEEY + KLS + 
Sbjct: 183 PQLPPQTADMKSGRIPKSGQMKSGFFGKSPKTQGEEEEDDPFAAEDLPEEYRKDKLSLWI 242

Query: 148 IFQLVLSVFVIVGLICNRWVSILKRETIWGLPLWKWELTVLAVICGHLVSILAVRLIVKI 207
           + + +  + +I G +C   +  L+++ +W L LWKWE  VL +ICG LVS   V+++V  
Sbjct: 243 VLEWLSLILIIAGFVCTLAIPSLRKKKLWELQLWKWESMVLVLICGRLVSSWIVKIVVFF 302

Query: 208 IEMNFLLRKRVLYFVYGLRNSVQICLWVSLILLVWHFIFNHKVQIKTQSKILPYVTKIFI 267
           IE NFLLRKRVLYFVYG+R +VQ CLW+ L+LL WHF+F+ KV     +K L  VTKIF+
Sbjct: 303 IERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLLAWHFLFDEKVAKAANTKALRVVTKIFV 362

Query: 268 CFLVGASIWLLKTLAIKILASFFHVNHYFERLKEALFSQFVIVSLSGLPL---------- 327
           C LVG  +WL+KTL +K+LAS FH++ YF+R++E+LF+Q+VI +LSG PL          
Sbjct: 363 CLLVGFLLWLVKTLLVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLIEIQKNEEEE 422

Query: 328 ---------FKRPNTKDVMMGDQNADGRE-ESPKSGRVICSGRLVDCNRSRPLKPDSVKE 387
                    F+ P   ++  G Q +  +  +SP    V+ +G                 E
Sbjct: 423 ERISVEVKKFQNPGGVEIQSGAQKSPMKTGKSPFLSHVLSNG------------GGGGGE 482

Query: 388 DEEIPVDQLNKMNQKNISAWNMRRMMNIIRKGALLTLDEKILSET-NEESLLQIRSERQA 447
           ++ I +D L+K+N KN+SAW M+R+MNIIR G+L TLDE++   + +++   QIRSE +A
Sbjct: 483 NKGITIDSLHKLNPKNVSAWKMKRLMNIIRNGSLTTLDEQLQDPSLDDDKGNQIRSEFEA 542

Query: 448 KEAAEKIFQRVVQRGSDCIYLEDVMRFMNKEEALMTLSLFGTTAETQCIDESSFREWMVN 507
           K AA KIF  V + GS  IY  D+MRF+  +EAL TLSLF   +ET  I +SS + W+VN
Sbjct: 543 KLAARKIFHNVAKPGSKFIYANDIMRFLPDDEALKTLSLFEGASETNRISKSSLKNWVVN 602

Query: 508 AFKQRKVLVLSLGDANTAVDDLHSLLNIFVAIIILVIWLIILGTPVRHFLIFITSQLLLL 567
           AF++R+ L L+L D  TAV+ LH ++NI V IIILVIWLIILG     FL+ ++SQ++++
Sbjct: 603 AFRERRALALTLNDTKTAVNRLHKMVNIVVGIIILVIWLIILGITSTKFLVVMSSQVVVV 662

Query: 568 VFIFGNTCKTVFEAIIFLFVMHPFDIGDRCEVDGVQMIVEEMKILTTVFLRYDNQKITYP 627
            FIFGN CK VFE+II+LFV+HPFD+GDRCE+DGVQM+VEEM ILTTVFLR+DNQK+ YP
Sbjct: 663 AFIFGNMCKIVFESIIYLFVIHPFDVGDRCEIDGVQMVVEEMNILTTVFLRFDNQKVVYP 722

Query: 628 NSVLATKAIGNYYRSPDMGEGVDFYVHISTPWEKVALLKERITRYVESMSELWHPNPQIV 687
           NS+L TK+IGNYYRSPDMG+G++F +HI+TP EK+ L+K+RIT Y+E   + W+P P IV
Sbjct: 723 NSLLWTKSIGNYYRSPDMGDGIEFSIHITTPAEKIILIKQRITSYIEGKKDHWYPAPMIV 782

Query: 688 MKEIEDLEKVKMSVCVTHKMNHQDMKERWNRRAVLVEEIVTI 690
            K++E L  V+++V  TH+MNHQDM E+W RR+ LVEEI  I
Sbjct: 783 FKDMESLNSVRIAVWPTHRMNHQDMGEKWARRSQLVEEIAKI 812

BLAST of Sed0028199 vs. ExPASy Swiss-Prot
Match: Q9LPG3 (Mechanosensitive ion channel protein 4 OS=Arabidopsis thaliana OX=3702 GN=MSL4 PE=3 SV=1)

HSP 1 Score: 562.4 bits (1448), Expect = 7.2e-159
Identity = 319/712 (44.80%), Postives = 449/712 (63.06%), Query Frame = 0

Query: 13  SFGNGGDKKIQGLPEKSPQTMRRRGKEFPEPETRRPGGGEVLRCSSYASFRGSSWSSPIS 72
           S+ NG    I+    +   +   +  +  E E  R  G EV++C+S  S   +     + 
Sbjct: 126 SYRNGTGDAIRRRQNRVTLSPSVKDGDSSEDEENRVDGSEVVKCTSNRSTMRTKTLMKMK 185

Query: 73  KRSRLMDPPEEDSSQKLETAVKDEGNNNNNDDDNNNQDDT------------------ED 132
            RSRLMDPP       +       GN N      N +  T                  ED
Sbjct: 186 TRSRLMDPPTPTYPDMVSGRTPRSGNLNPGFSGRNTKPGTPNQGGSKDLEEEEDPFSEED 245

Query: 133 MPEEYDQLKLSPFFIFQLVLSVFVIVGLICNRWVSILKRETIWGLPLWKWELTVLAVICG 192
           +PE   + K+  + I + +  + +I  LIC+  +  L+ +T+W L LWKWE+ VL +ICG
Sbjct: 246 LPEGLRKEKICVWVIIEWIFLILIIASLICSLVIPYLRGKTLWDLALWKWEVMVLVLICG 305

Query: 193 HLVSILAVRLIVKIIEMNFLLRKRVLYFVYGLRNSVQICLWVSLILLVWHFIFNHKVQIK 252
            LVS   V+L V  +E NFL RK+VLYFVYG+R  VQ CLW+ L+L+ WHF+F+ KV+ +
Sbjct: 306 RLVSSWIVKLFVYFVESNFLWRKKVLYFVYGIRKPVQNCLWLGLVLIAWHFLFDKKVERE 365

Query: 253 TQSKILPYVTKIFICFLVGASIWLLKTLAIKILASFFHVNHYFERLKEALFSQFVIVSLS 312
            +S +L YVTK+ IC LV   IWL+KTL +K+LAS FH++ YF+R++E+LF+Q+VI +LS
Sbjct: 366 MRSTVLKYVTKVLICLLVAVIIWLIKTLLVKVLASSFHMSTYFDRIQESLFTQYVIETLS 425

Query: 313 GLPLFKRPNTKDVMMGDQNA---DGREESPKSGRVICSGRLVDCNRSRPLK--------- 372
           G P  +    ++ +  D       GR+ SP   + + S   V     R  K         
Sbjct: 426 GPPRIEIHIEEEKVANDVKTFEIVGRKLSPLGPKAVSSPPQVTVGSGRLQKSPSRVGKSP 485

Query: 373 ----PDSVKE--DEEIPVDQLNKMNQKNISAWNMRRMMNIIRKGALLTLDEKILSETNEE 432
                 S KE  +E I +D L +MN KN+SAW M+++MN+I+KG L TLDE+I   T +E
Sbjct: 486 VLSRSGSKKEGGEEGIRIDHLQRMNTKNVSAWKMKKLMNVIKKGTLSTLDEQIQDTTTQE 545

Query: 433 --SLLQIRSERQAKEAAEKIFQRVVQRGSDCIYLEDVMRFMNKEEALMTLSLFGTTAETQ 492
                QIRSE +AK AA KIFQ V + GS  IY+ED MRF++++E+   + LF   +E  
Sbjct: 546 DDKATQIRSEFEAKLAARKIFQNVAEPGSRYIYMEDFMRFLSEDESERAMDLFEGASECH 605

Query: 493 CIDESSFREWMVNAFKQRKVLVLSLGDANTAVDDLHSLLNIFVAIIILVIWLIILGTPVR 552
            I +S  + W+VNAF++R+ L L+L D  TAV+ LH ++++ V+I+IL+IWL+ILG    
Sbjct: 606 KISKSCLKNWVVNAFRERRALALTLNDTKTAVNRLHRIVDVLVSIVILIIWLLILGIATT 665

Query: 553 HFLIFITSQLLLLVFIFGNTCKTVFEAIIFLFVMHPFDIGDRCEVDGVQMIVEEMKILTT 612
            FL+ I+SQLLL+VF+FGN+CKT+FEA+IF+FVMHPFD+GDRCE+DGVQMIVEEM ILTT
Sbjct: 666 KFLLVISSQLLLVVFVFGNSCKTIFEAVIFVFVMHPFDVGDRCEIDGVQMIVEEMNILTT 725

Query: 613 VFLRYDNQKITYPNSVLATKAIGNYYRSPDMGEGVDFYVHISTPWEKVALLKERITRYVE 672
           VFLR+DNQKI YPNS+L TK I NYYRSPDM + ++F+VHI+TP EK   L++RI  YV+
Sbjct: 726 VFLRFDNQKIVYPNSLLGTKPIANYYRSPDMQDAIEFFVHIATPPEKTTALRQRILSYVD 785

Query: 673 SMSELWHPNPQIVMKEIEDLEKVKMSVCVTHKMNHQDMKERWNRRAVLVEEI 687
           +  + WHP+P IV +++  L  VK+++  THKMNHQ+M ER+ RR  L+EEI
Sbjct: 786 NKKDHWHPSPMIVFRDMCGLNSVKIAMWPTHKMNHQNMGERYVRRGQLLEEI 837

BLAST of Sed0028199 vs. ExPASy Swiss-Prot
Match: Q9LH74 (Mechanosensitive ion channel protein 5 OS=Arabidopsis thaliana OX=3702 GN=MSL5 PE=2 SV=1)

HSP 1 Score: 561.6 bits (1446), Expect = 1.2e-158
Identity = 315/680 (46.32%), Postives = 438/680 (64.41%), Query Frame = 0

Query: 42  EPETRRPGGGEVLRCSSYASFRGSSWSSPISKRSRLMDPPEEDSSQKLETAVKD------ 101
           + E R     EVL+C S       +       +SRL DPP        +T +K       
Sbjct: 168 DEEGRNRDEAEVLKCGSKKPMLSRN-----KTKSRLQDPPTPTHPAIDKTEMKSGRRSGI 227

Query: 102 --------------EGNNNNNDDDNNNQDDTEDMPEEYDQLKLSPFFIFQLVLSVFVIVG 161
                          G N   +++  +    ED+PEE+ + KLS +   + +  V ++  
Sbjct: 228 FKSGFLGKSPKAGTPGRNGFEEEEEEDPFLDEDLPEEFKRDKLSFWVFLEWISLVLIVTS 287

Query: 162 LICNRWVSILKRETIWGLPLWKWELTVLAVICGHLVSILAVRLIVKIIEMNFLLRKRVLY 221
           L+C+  +  L+R+T W L LWKWE+TVL +ICG LVS   VR+IV ++E NF  RKRVLY
Sbjct: 288 LVCSLTIHNLQRKTWWKLDLWKWEVTVLVLICGRLVSSWIVRIIVFLVEKNFTWRKRVLY 347

Query: 222 FVYGLRNSVQICLWVSLILLVWHFIFNHKVQIKTQSKILPYVTKIFICFLVGASIWLLKT 281
           FVYG+R SVQ CLW+ L+LL WHF+F+ KV+ +T+S  L YVT++ +C LV   IWL+KT
Sbjct: 348 FVYGVRKSVQNCLWLGLVLLAWHFLFDKKVERETRSTALRYVTRVLVCLLVALIIWLVKT 407

Query: 282 LAIKILASFFHVNHYFERLKEALFSQFVIVSLSGLPLFKRPNTKDVMMGDQNADGREESP 341
           + +K+LAS FH++ YF+R++E+LF+Q+VI +LSG PL       ++   ++      E  
Sbjct: 408 ILVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLM------EIQRMEEEEQQVAEDV 467

Query: 342 KSGRVICSGRL-----------VDCNRSRPLKPDSVKEDEE---IPVDQLNKMNQKNISA 401
           KS   +   +L           +   +S  L     K  E+   I +DQL +MN KN+SA
Sbjct: 468 KSLEKLAGAKLPPALKATVKSFMKVGKSPGLNRIGSKRGEDGEGIRIDQLKRMNTKNVSA 527

Query: 402 WNMRRMMNIIRKGALLTLDEKILSETNE-ESLLQIRSERQAKEAAEKIFQRVVQRGSDCI 461
           WNM+R+MNII KGA+ TLD+ +   T E E    IRSE +AK AA KIF  V + GS  I
Sbjct: 528 WNMKRLMNIILKGAISTLDQNMQDTTQEDEDATHIRSEYEAKCAARKIFHNVTEPGSRYI 587

Query: 462 YLEDVMRFMNKEEALMTLSLFGTTAETQCIDESSFREWMVNAFKQRKVLVLSLGDANTAV 521
           YLED +RF+ +EEA   ++LF   +E+  I +S  + W+V AF++R+ L L+L D  TAV
Sbjct: 588 YLEDFLRFLCEEEAERAMALFEGASESDKISKSCLKNWVVKAFRERRALALTLNDTKTAV 647

Query: 522 DDLHSLLNIFVAIIILVIWLIILGTPVRHFLIFITSQLLLLVFIFGNTCKTVFEAIIFLF 581
           D LH ++N+ + III++IWL+ILG     FL+ ++SQLLL+ F+FGN+CKT+FEAIIFLF
Sbjct: 648 DRLHRIINVVIGIIIIIIWLLILGIATTRFLLVLSSQLLLVAFVFGNSCKTIFEAIIFLF 707

Query: 582 VMHPFDIGDRCEVDGVQMIVEEMKILTTVFLRYDNQKITYPNSVLATKAIGNYYRSPDMG 641
           VMHPFD+GDRCE+DGVQ++VEEM ILTTVFLRYDNQKI YPNSVL TK I NYYRSPDMG
Sbjct: 708 VMHPFDVGDRCEIDGVQLVVEEMNILTTVFLRYDNQKIIYPNSVLGTKPIANYYRSPDMG 767

Query: 642 EGVDFYVHISTPWEKVALLKERITRYVESMSELWHPNPQIVMKEIEDLEKVKMSVCVTHK 687
           + V+F VHI+TP EK+  +K+RI  YV++  + W+P P IV   ++DL  VK++V +TH+
Sbjct: 768 DAVEFCVHIATPPEKITAIKQRILSYVDNKKDYWYPAPMIVFLSMDDLNSVKIAVWLTHR 827

BLAST of Sed0028199 vs. ExPASy Swiss-Prot
Match: F4IME1 (Mechanosensitive ion channel protein 7 OS=Arabidopsis thaliana OX=3702 GN=MSL7 PE=2 SV=1)

HSP 1 Score: 545.8 bits (1405), Expect = 7.0e-154
Identity = 324/716 (45.25%), Postives = 467/716 (65.22%), Query Frame = 0

Query: 3   SKE-EVFLKIDSFG----NGGDKKIQGLPEKSPQTMRRRGKEFPEPETRRPGGGEVLRCS 62
           SKE  VF KI+S G    +G  +        S  TMR   ++  E E     G  V+RCS
Sbjct: 104 SKETRVFFKINSSGTNNMSGSVRSCTSSTSFSSATMRLNLEQQLEDE-----GEVVVRCS 163

Query: 63  SYASFRGSSWSSPISKRSRLMDPPEEDSSQKLE-TAVKDE---GNNNNNDDDNNNQDDT- 122
              S R +   S    RSRL+DPP+E+  Q        D+   G    + DD   +DD+ 
Sbjct: 164 ---SVRKTELVSRAKARSRLIDPPQEEEQQYSSWIGTSDQLRSGLLGRHSDDIEEEDDSS 223

Query: 123 --EDMPEEYDQLKLSPFFIFQLVLSVFVIVGLICNRWVSILKRETIWGLPLWKWELTVLA 182
             ED+P EY +LK+    + Q +  + ++V L+ +  +   +  T+W L LWKWE+ +L 
Sbjct: 224 AEEDVPVEYRKLKMDAITLLQWMSLIALVVALVLSLGLHTWRNATLWSLHLWKWEVVLLV 283

Query: 183 VICGHLVSILAVRLIVKIIEMNFLLRKRVLYFVYGLRNSVQICLWVSLILLVWHFIFNHK 242
           +ICG LVS   +R+IV  IE NFLLRKRVLYFVYG++ +VQ CLW+ L+LL WHF+F+ K
Sbjct: 284 LICGRLVSGCGIRIIVFFIERNFLLRKRVLYFVYGVKTAVQNCLWLGLVLLAWHFLFDKK 343

Query: 243 VQIKTQSKILPYVTKIFICFLVGASIWLLKTLAIKILASFFHVNHYFERLKEALFSQFVI 302
           V+ +TQS +L  ++KI +CFL+   +WL+KTL +K+LAS FHV+ YF+R++EALF  ++I
Sbjct: 344 VEKETQSDVLLLMSKILVCFLLSTVLWLIKTLVVKVLASSFHVSTYFDRIQEALFHHYLI 403

Query: 303 VSLSGLPLF-------KRPNTKDVMMGDQNADGREESPKSGRVIC--------SGRLVDC 362
            +LSG P+        +   T+D +   Q   G + SP+    +C        SG  ++ 
Sbjct: 404 ETLSGPPMLELSRIEEEEDRTQDEIYKMQKG-GADLSPE----LCSAAFPQEKSGSTMNM 463

Query: 363 NRSRPLKPDSVKEDEEIPVDQLNKMNQKNISAWNMRRMMNIIRKGALLTLDEKILSET-N 422
             S P+ P     D  I +D L+KMNQKN+SAWNM+R+M I+R  +L TLDE+ L  T  
Sbjct: 464 KFS-PIIP-KTGSDNGITMDDLHKMNQKNVSAWNMKRLMKIVRNVSLSTLDEQALQNTCE 523

Query: 423 EESLLQIRSERQAKEAAEKIFQRVVQRGSDCIYLEDVMRFMNKEEALMTLSLFGTTAETQ 482
           +ES  QIRSE++AK AA KIF+ V Q G+  IYLED+MRF+  +EA+ T+ LF     T+
Sbjct: 524 DESTRQIRSEKEAKAAARKIFKNVAQPGTKHIYLEDLMRFLRVDEAMKTMCLFEGALVTK 583

Query: 483 CIDESSFREWMVNAFKQRKVLVLSLGDANTAVDDLHSLLNIFVAIIILVIWLIILGTPVR 542
            I +S+ + W+VNAF++R+ L L+L D  TAV+ LH +++   AI+I+VIWLI+L     
Sbjct: 584 KITKSALKNWLVNAFRERRALALTLNDTKTAVNKLHHMISFLTAIVIIVIWLILLEIATS 643

Query: 543 HFLIFITSQLLLLVFIFGNTCKTVFEAIIFLFVMHPFDIGDRCEVDGVQMIVEEMKILTT 602
            +L+F+TSQ++LL F+FGN+ KTVFE+IIFLF++HP+D+GDR  +D V+M+VEEM ILTT
Sbjct: 644 KYLLFLTSQVVLLAFMFGNSLKTVFESIIFLFIIHPYDVGDRLLIDTVEMVVEEMNILTT 703

Query: 603 VFLRYDNQKITYPNSVLATKAIGNYYRSPDMGEGVDFYVHISTPWEKVALLKERITRYVE 662
           VFLR DN KI YPN +L  KAI NY RSPDMG+ V   VHI+TP EK+A +K+RI+ Y++
Sbjct: 704 VFLRADNLKIVYPNILLWQKAIHNYNRSPDMGDEVTCCVHITTPPEKIAAIKQRISSYID 763

Query: 663 SMSELWHPNPQIVMKEIEDLEKVKMSVCVTHKMNHQDMKERWNRRAVLVEEIVTIL 691
           S  E W+P   +++K++EDL  V++++ + HK+NHQ+M ER+ RRA+L+EE++ IL
Sbjct: 764 SKPEYWYPKADVIVKDVEDLNIVRIAIWLCHKINHQNMGERFTRRALLIEEVIKIL 804

BLAST of Sed0028199 vs. ExPASy TrEMBL
Match: A0A6J1F811 (Mechanosensitive ion channel protein OS=Cucurbita moschata OX=3662 GN=LOC111442956 PE=3 SV=1)

HSP 1 Score: 1109.7 bits (2869), Expect = 0.0e+00
Identity = 574/701 (81.88%), Postives = 634/701 (90.44%), Query Frame = 0

Query: 1   MASKEEVFLKIDSFGNGG----DKKIQGLPEKSPQTMRRRGKEFPEPETRRP----GGGE 60
           MA KE+V LK++SFGNG     +KK Q LPE+S QTMRRRGKEF EPE R+P     GGE
Sbjct: 1   MAPKEDVILKVNSFGNGDKELVEKKFQCLPERSLQTMRRRGKEFSEPENRQPSSGSSGGE 60

Query: 61  VLRCSSYASFRGSSWSSPISKRSRLMDPPEEDSSQKLETAVKDEGNNNNNDDDNNNQDDT 120
           VLRCSS  SF GSSWSSPISK+SRLMDPPE+DSSQKLETAVKDEGNN      NNNQD T
Sbjct: 61  VLRCSSNYSFSGSSWSSPISKKSRLMDPPEDDSSQKLETAVKDEGNN------NNNQDGT 120

Query: 121 EDMPEEYDQLKLSPFFIFQLVLSVFVIVGLICNRWVSILKRETIWGLPLWKWELTVLAVI 180
           EDMPEEY+QLKLSP   FQLV+ V +IV LICNRW+SILKRET WGLPLWKWELTVLAVI
Sbjct: 121 EDMPEEYNQLKLSPLLGFQLVVFVLIIVSLICNRWISILKRETFWGLPLWKWELTVLAVI 180

Query: 181 CGHLVSILAVRLIVKIIEMNFLLRKRVLYFVYGLRNSVQICLWVSLILLVWHFIFNHKVQ 240
           CGH+VS +AV L+VKIIEMNFLLRKRVLYFVYGLR +V+ CLW+SLILLVWHFIFN KVQ
Sbjct: 181 CGHMVSSMAVGLMVKIIEMNFLLRKRVLYFVYGLRKAVKNCLWMSLILLVWHFIFNDKVQ 240

Query: 241 IKTQSKILPYVTKIFICFLVGASIWLLKTLAIKILASFFHVNHYFERLKEALFSQFVIVS 300
            KTQSKILPYVTKI ICFLV ASIWLLKTL IKILASFFHVN+YFERLKEALFSQ+VIV+
Sbjct: 241 RKTQSKILPYVTKILICFLVAASIWLLKTLVIKILASFFHVNNYFERLKEALFSQYVIVT 300

Query: 301 LSGLPLFKRPNTKDVMMGDQNADGREESPKSGRVICSGRLVDCNRSRPLKPDSVKEDEEI 360
           LSGLPLF+RPN++D+M  +Q  DG+ ES KSGRV+CSGRLV+CN  R LK +S ++DEEI
Sbjct: 301 LSGLPLFERPNSEDMMKDEQIGDGKAESSKSGRVVCSGRLVNCNGWRQLKVESKRKDEEI 360

Query: 361 PVDQLNKMNQKNISAWNMRRMMNIIRKGALLTLDEKILSETNEESLLQIRSERQAKEAAE 420
           PVD+LNK+NQKNISAWNMRRMMNI+R GALLTLDEKILSET E+SLLQIR+ERQAKEAA 
Sbjct: 361 PVDELNKLNQKNISAWNMRRMMNIVRNGALLTLDEKILSETEEQSLLQIRNERQAKEAAG 420

Query: 421 KIFQRVVQRGSDCIYLEDVMRFMNKEEALMTLSLFGTTAETQCIDESSFREWMVNAFKQR 480
           +IF+RV QRGSDCIY+EDVMRFMNKEEAL+T+SLFGTTAETQCIDESSFREWMVNAFKQR
Sbjct: 421 RIFRRVAQRGSDCIYIEDVMRFMNKEEALVTMSLFGTTAETQCIDESSFREWMVNAFKQR 480

Query: 481 KVLVLSLGDANTAVDDLHSLLNIFVAIIILVIWLIILGTPVRHFLIFITSQLLLLVFIFG 540
           KVLVLSL DANTAVDDLH+LLN+FVA+ ILVIWLII GTPV HFLIFITSQLLLL FIFG
Sbjct: 481 KVLVLSLNDANTAVDDLHNLLNVFVALTILVIWLIIFGTPVLHFLIFITSQLLLLAFIFG 540

Query: 541 NTCKTVFEAIIFLFVMHPFDIGDRCEVDGVQMIVEEMKILTTVFLRYDNQKITYPNSVLA 600
           NTCKTVFEAIIFLFVMHPFD+GDRCEVDGV+M+VEEMKILTTVFLRYDNQ+I+YPNSVLA
Sbjct: 541 NTCKTVFEAIIFLFVMHPFDVGDRCEVDGVEMVVEEMKILTTVFLRYDNQRISYPNSVLA 600

Query: 601 TKAIGNYYRSPDMGEGVDFYVHISTPWEKVALLKERITRYVESMSELWHPNPQIVMKEIE 660
           TKAIGNYYRSPDMGEGVDFY+HISTPWEKVALLKERITRYVESM E WHP+PQIVMK++E
Sbjct: 601 TKAIGNYYRSPDMGEGVDFYIHISTPWEKVALLKERITRYVESMGEQWHPDPQIVMKDVE 660

Query: 661 DLEKVKMSVCVTHKMNHQDMKERWNRRAVLVEEIVTILEST 694
           DLEKV+MSV +THK+NHQ+MKERWNRRA LV+EIVTIL+ST
Sbjct: 661 DLEKVRMSVGLTHKLNHQNMKERWNRRAALVQEIVTILDST 695

BLAST of Sed0028199 vs. ExPASy TrEMBL
Match: A0A6J1IGQ2 (Mechanosensitive ion channel protein OS=Cucurbita maxima OX=3661 GN=LOC111474765 PE=3 SV=1)

HSP 1 Score: 1109.4 bits (2868), Expect = 0.0e+00
Identity = 573/701 (81.74%), Postives = 636/701 (90.73%), Query Frame = 0

Query: 1   MASKEEVFLKIDSFGNGG----DKKIQGLPEKSPQTMRRRGKEFPEPETRR----PGGGE 60
           MA KE+V LK++SFG+G     +KK Q LPE+S QTMRRRGKEF EPE R+      GGE
Sbjct: 1   MAPKEDVILKVNSFGHGDKELVEKKFQCLPERSLQTMRRRGKEFSEPENRQLSSGSSGGE 60

Query: 61  VLRCSSYASFRGSSWSSPISKRSRLMDPPEEDSSQKLETAVKDEGNNNNNDDDNNNQDDT 120
           VLRCSS +SF GSSWSSPISK+SRLMDPPE+DSSQKLETAVKDEGNN      NNNQD T
Sbjct: 61  VLRCSSNSSFPGSSWSSPISKKSRLMDPPEDDSSQKLETAVKDEGNN------NNNQDGT 120

Query: 121 EDMPEEYDQLKLSPFFIFQLVLSVFVIVGLICNRWVSILKRETIWGLPLWKWELTVLAVI 180
           EDMPEEY+QLKLSP   FQLV+ V +IV LICNRW+SILKRET WGLPLWKWELTVLAVI
Sbjct: 121 EDMPEEYNQLKLSPLLGFQLVVFVLIIVSLICNRWISILKRETFWGLPLWKWELTVLAVI 180

Query: 181 CGHLVSILAVRLIVKIIEMNFLLRKRVLYFVYGLRNSVQICLWVSLILLVWHFIFNHKVQ 240
           CGH+VS +AV L+VKIIEMNFLLRKRVLYFVYGLR +V+ CLW+SLILLVWHFIFN KVQ
Sbjct: 181 CGHMVSSMAVGLMVKIIEMNFLLRKRVLYFVYGLRKAVKNCLWMSLILLVWHFIFNDKVQ 240

Query: 241 IKTQSKILPYVTKIFICFLVGASIWLLKTLAIKILASFFHVNHYFERLKEALFSQFVIVS 300
            KTQSKILPYVTKI ICFLV A+IWLLKTL IKILASFFHVN+YFERLKEALFSQ+VIV+
Sbjct: 241 RKTQSKILPYVTKILICFLVAATIWLLKTLVIKILASFFHVNNYFERLKEALFSQYVIVT 300

Query: 301 LSGLPLFKRPNTKDVMMGDQNADGREESPKSGRVICSGRLVDCNRSRPLKPDSVKEDEEI 360
           LSGLPLF+RPN++D+M  +Q ADG+ ES KSGRV+CSGRLV+CN SR LK +S ++DEEI
Sbjct: 301 LSGLPLFERPNSEDMMKDEQIADGKAESSKSGRVVCSGRLVNCNGSRQLKVESKRKDEEI 360

Query: 361 PVDQLNKMNQKNISAWNMRRMMNIIRKGALLTLDEKILSETNEESLLQIRSERQAKEAAE 420
           PVD+LNK+NQKNISAWNMRRMMNI+R GALLTLDEKILSET E+SLLQIRSERQAKEAA 
Sbjct: 361 PVDELNKLNQKNISAWNMRRMMNIVRNGALLTLDEKILSETKEQSLLQIRSERQAKEAAG 420

Query: 421 KIFQRVVQRGSDCIYLEDVMRFMNKEEALMTLSLFGTTAETQCIDESSFREWMVNAFKQR 480
           +IF+RV QRGSDCIY+EDVMRFMNKEEAL+T+SLFGTTAETQCIDESSFREWMVNAFKQR
Sbjct: 421 RIFRRVAQRGSDCIYIEDVMRFMNKEEALVTMSLFGTTAETQCIDESSFREWMVNAFKQR 480

Query: 481 KVLVLSLGDANTAVDDLHSLLNIFVAIIILVIWLIILGTPVRHFLIFITSQLLLLVFIFG 540
           KVLVLSL DANTAVDDLH+LLN+FVA+ ILVIWLI+ GTPV HFLIFITSQLLLL FIFG
Sbjct: 481 KVLVLSLNDANTAVDDLHNLLNVFVALTILVIWLIVFGTPVLHFLIFITSQLLLLAFIFG 540

Query: 541 NTCKTVFEAIIFLFVMHPFDIGDRCEVDGVQMIVEEMKILTTVFLRYDNQKITYPNSVLA 600
           NTCKTVFEAIIFLFVMHPFD+GDRCEVDGV+M+VEEMKILTTVFLRYDNQ+I+YPNSVLA
Sbjct: 541 NTCKTVFEAIIFLFVMHPFDVGDRCEVDGVEMVVEEMKILTTVFLRYDNQRISYPNSVLA 600

Query: 601 TKAIGNYYRSPDMGEGVDFYVHISTPWEKVALLKERITRYVESMSELWHPNPQIVMKEIE 660
           TKAIGNYYRSPDMGEGVDFY+HISTPWEKVALLKERITRYVESM E WHP+PQIVMK++E
Sbjct: 601 TKAIGNYYRSPDMGEGVDFYIHISTPWEKVALLKERITRYVESMGEQWHPDPQIVMKDVE 660

Query: 661 DLEKVKMSVCVTHKMNHQDMKERWNRRAVLVEEIVTILEST 694
           DLEKV+MSV +THK+NHQ+MKERWNRRA LV+EIVTIL+ST
Sbjct: 661 DLEKVRMSVGLTHKLNHQNMKERWNRRAALVQEIVTILDST 695

BLAST of Sed0028199 vs. ExPASy TrEMBL
Match: A0A6J1CGD4 (Mechanosensitive ion channel protein OS=Momordica charantia OX=3673 GN=LOC111011429 PE=3 SV=1)

HSP 1 Score: 1065.1 bits (2753), Expect = 1.3e-307
Identity = 557/698 (79.80%), Postives = 615/698 (88.11%), Query Frame = 0

Query: 1   MASKEEVFLKIDSFGNGGDKK-----IQGLPEKSPQTMRRRGKEFPEPETRRP----GGG 60
           MA +E+V LKIDSFGNG DK+      Q LPE+S QTMRRRGKE  + E RRP    GGG
Sbjct: 1   MAPREDVLLKIDSFGNGADKESEEKNFQCLPERSAQTMRRRGKESSDSENRRPSSASGGG 60

Query: 61  EVLRCSSYASFRGSSWSSPISKRSRLMDPPEEDSSQKLETAVKDEGNNNNNDDDNNNQDD 120
           EVLRCSS ASFRG SWSSPISK+SRLMDPPEEDSSQKLETAV +EG          NQD+
Sbjct: 61  EVLRCSSNASFRGCSWSSPISKKSRLMDPPEEDSSQKLETAVSEEG---------KNQDE 120

Query: 121 TEDMPEEYDQLKLSPFFIFQLVLSVFVIVGLICNRWVSILKRETIWGLPLWKWELTVLAV 180
           TEDMPEEY+QLKLSPF + Q V+ V VIVGLICNRWV ILKR++IW LPLWKWELTVLA+
Sbjct: 121 TEDMPEEYNQLKLSPFSVLQWVIFVIVIVGLICNRWVPILKRKSIWDLPLWKWELTVLAL 180

Query: 181 ICGHLVSILAVRLIVKIIEMNFLLRKRVLYFVYGLRNSVQICLWVSLILLVWHFIFNHKV 240
           ICG L+S  AV LIVKIIE NFLLRKRVLYFVYGLR +V+ CLW+SL+LLVWHFIFN KV
Sbjct: 181 ICGRLISTWAVGLIVKIIERNFLLRKRVLYFVYGLRRAVRNCLWLSLLLLVWHFIFNEKV 240

Query: 241 QIKTQSKILPYVTKIFICFLVGASIWLLKTLAIKILASFFHVNHYFERLKEALFSQFVIV 300
           Q  T+SKILPY+TKI ICFLVGASIWLLKTLA+KILASFFHVN+YFERLKEALFSQ+VIV
Sbjct: 241 QRTTRSKILPYITKILICFLVGASIWLLKTLAVKILASFFHVNNYFERLKEALFSQYVIV 300

Query: 301 SLSGLPLFKRPNTKDVMMGDQNADGREESPKSGRVICSGRLVDCNRSRPLKPDSVKEDEE 360
           +LSG PLF+R NT+D++  +QNADGREESPKSGR+ICSGRLV+C+ SR LKP+S ++DEE
Sbjct: 301 TLSGSPLFERSNTEDMVKDNQNADGREESPKSGRIICSGRLVNCDGSRQLKPESSRQDEE 360

Query: 361 IPVDQLNKMNQKNISAWNMRRMMNIIRKGALLTLDEKILSETNEESLLQIRSERQAKEAA 420
           IPVDQLNK+NQ NISAWNMRRMMNI+R GALLTLDEKILSET EE LLQIRSERQAKEAA
Sbjct: 361 IPVDQLNKLNQHNISAWNMRRMMNIVRNGALLTLDEKILSETKEEYLLQIRSERQAKEAA 420

Query: 421 EKIFQRVVQRGSDCIYLEDVMRFMNKEEALMTLSLFGTTAETQCIDESSFREWMVNAFKQ 480
             IF+RV +RGS+ I L+DVMRFMNKEEALMT+SLFG  AETQCIDE SFREWMVNAF Q
Sbjct: 421 GNIFRRVSRRGSEFINLDDVMRFMNKEEALMTMSLFGPDAETQCIDEFSFREWMVNAFNQ 480

Query: 481 RKVLVLSLGDANTAVDDLHSLLNIFVAIIILVIWLIILGTPVRHFLIFITSQLLLLVFIF 540
           RKVLVLSL DANTAVD LH+LLNIFVA+ ILVIWLIILGTP+ HFLIFI+SQLLLLVFIF
Sbjct: 481 RKVLVLSLNDANTAVDGLHNLLNIFVAVTILVIWLIILGTPILHFLIFISSQLLLLVFIF 540

Query: 541 GNTCKTVFEAIIFLFVMHPFDIGDRCEVDGVQMIVEEMKILTTVFLRYDNQKITYPNSVL 600
           GNTCKTVFEAIIFLFVMHPFD+GDRCEVDGVQM+VEEM+ILTTVFLRYDNQKITYPNSVL
Sbjct: 541 GNTCKTVFEAIIFLFVMHPFDVGDRCEVDGVQMVVEEMRILTTVFLRYDNQKITYPNSVL 600

Query: 601 ATKAIGNYYRSPDMGEGVDFYVHISTPWEKVALLKERITRYVESMSELWHPNPQIVMKEI 660
           ATKAIGNYYRSPDMGEGVDFYVHISTP +KV+L+KE+ITRY+ES SE WHP P + MK++
Sbjct: 601 ATKAIGNYYRSPDMGEGVDFYVHISTPSKKVSLIKEQITRYIESKSEEWHPAPVVTMKDV 660

Query: 661 EDLEKVKMSVCVTHKMNHQDMKERWNRRAVLVEEIVTI 690
           EDLEKVKMSVCVTHKMNHQDM ERWNRRA LVEEIV I
Sbjct: 661 EDLEKVKMSVCVTHKMNHQDMGERWNRRAALVEEIVKI 689

BLAST of Sed0028199 vs. ExPASy TrEMBL
Match: A0A6J1CH06 (Mechanosensitive ion channel protein OS=Momordica charantia OX=3673 GN=LOC111011429 PE=3 SV=1)

HSP 1 Score: 991.5 bits (2562), Expect = 1.8e-285
Identity = 526/698 (75.36%), Postives = 582/698 (83.38%), Query Frame = 0

Query: 1   MASKEEVFLKIDSFGNGGDKK-----IQGLPEKSPQTMRRRGKEFPEPETRRP----GGG 60
           MA +E+V LKIDSFGNG DK+      Q LPE+S QTMRRRGKE  + E RRP    GGG
Sbjct: 1   MAPREDVLLKIDSFGNGADKESEEKNFQCLPERSAQTMRRRGKESSDSENRRPSSASGGG 60

Query: 61  EVLRCSSYASFRGSSWSSPISKRSRLMDPPEEDSSQKLETAVKDEGNNNNNDDDNNNQDD 120
           EVLRCSS ASFRG SWSSPISK+SRLMDPPEEDSSQKLETAV +EG          NQD+
Sbjct: 61  EVLRCSSNASFRGCSWSSPISKKSRLMDPPEEDSSQKLETAVSEEG---------KNQDE 120

Query: 121 TEDMPEEYDQLKLSPFFIFQLVLSVFVIVGLICNRWVSILKRETIWGLPLWKWELTVLAV 180
           TEDMPEEY+QLKLSPF + Q V+ V VIVGLICNRWV ILKR++IW LPLWKWELTVLA+
Sbjct: 121 TEDMPEEYNQLKLSPFSVLQWVIFVIVIVGLICNRWVPILKRKSIWDLPLWKWELTVLAL 180

Query: 181 ICGHLVSILAVRLIVKIIEMNFLLRKRVLYFVYGLRNSVQICLWVSLILLVWHFIFNHKV 240
           ICG L+S  AV LIVKIIE NFLLRKRVLYFVYGLR +V+ CLW+SL+LLVWHFIFN KV
Sbjct: 181 ICGRLISTWAVGLIVKIIERNFLLRKRVLYFVYGLRRAVRNCLWLSLLLLVWHFIFNEKV 240

Query: 241 QIKTQSKILPYVTKIFICFLVGASIWLLKTLAIKILASFFHVNHYFERLKEALFSQFVIV 300
           Q  T+SKILPY+TKI ICFLVGASIWLLKTLA+KILASFFHVN+YFERLKEALFSQ+VIV
Sbjct: 241 QRTTRSKILPYITKILICFLVGASIWLLKTLAVKILASFFHVNNYFERLKEALFSQYVIV 300

Query: 301 SLSGLPLFKRPNTKDVMMGDQNADGREESPKSGRVICSGRLVDCNRSRPLKPDSVKEDEE 360
           +LSG PLF+R NT+D++  +QNADGREESPKSGR+ICSGRLV+C+ SR LKP+S ++DEE
Sbjct: 301 TLSGSPLFERSNTEDMVKDNQNADGREESPKSGRIICSGRLVNCDGSRQLKPESSRQDEE 360

Query: 361 IPVDQLNKMNQKNISAWNMRRMMNIIRKGALLTLDEKILSETNEESLLQIRSERQAKEAA 420
           IPVDQLNK+NQ NISAWNMRRMMNI+R GALLTLDEKILSET EE LLQIRSERQAKEAA
Sbjct: 361 IPVDQLNKLNQHNISAWNMRRMMNIVRNGALLTLDEKILSETKEEYLLQIRSERQAKEAA 420

Query: 421 EKIFQRVVQRGSDCIYLEDVMRFMNKEEALMTLSLFGTTAETQCIDESSFREWMVNAFKQ 480
             IF+RV +RGS+ I L+DVMRFMNKEEALMT+SLFG  AETQCIDE SFREWMVNAF Q
Sbjct: 421 GNIFRRVSRRGSEFINLDDVMRFMNKEEALMTMSLFGPDAETQCIDEFSFREWMVNAFNQ 480

Query: 481 RKVLVLSLGDANTAVDDLHSLLNIFVAIIILVIWLIILGTPVRHFLIFITSQLLLLVFIF 540
           RKVLVLSL DANTAVD LH+LLNIFVA+ IL                             
Sbjct: 481 RKVLVLSLNDANTAVDGLHNLLNIFVAVTIL----------------------------- 540

Query: 541 GNTCKTVFEAIIFLFVMHPFDIGDRCEVDGVQMIVEEMKILTTVFLRYDNQKITYPNSVL 600
                TVFEAIIFLFVMHPFD+GDRCEVDGVQM+VEEM+ILTTVFLRYDNQKITYPNSVL
Sbjct: 541 -----TVFEAIIFLFVMHPFDVGDRCEVDGVQMVVEEMRILTTVFLRYDNQKITYPNSVL 600

Query: 601 ATKAIGNYYRSPDMGEGVDFYVHISTPWEKVALLKERITRYVESMSELWHPNPQIVMKEI 660
           ATKAIGNYYRSPDMGEGVDFYVHISTP +KV+L+KE+ITRY+ES SE WHP P + MK++
Sbjct: 601 ATKAIGNYYRSPDMGEGVDFYVHISTPSKKVSLIKEQITRYIESKSEEWHPAPVVTMKDV 655

Query: 661 EDLEKVKMSVCVTHKMNHQDMKERWNRRAVLVEEIVTI 690
           EDLEKVKMSVCVTHKMNHQDM ERWNRRA LVEEIV I
Sbjct: 661 EDLEKVKMSVCVTHKMNHQDMGERWNRRAALVEEIVKI 655

BLAST of Sed0028199 vs. ExPASy TrEMBL
Match: A0A5E4FCX0 (Mechanosensitive ion channel protein OS=Prunus dulcis OX=3755 GN=ALMOND_2B014830 PE=3 SV=1)

HSP 1 Score: 749.2 bits (1933), Expect = 1.5e-212
Identity = 414/724 (57.18%), Postives = 532/724 (73.48%), Query Frame = 0

Query: 1   MASKE-EVFLKIDSFGNGGDKK-------------IQGLPEKSPQTMRRRGKEFPEPET- 60
           MA +E +V +KIDS G    K+             + G PEK  ++ RRR KE P P+  
Sbjct: 1   MAQREGQVVVKIDSCGASCSKETTKINGEATPTTTVGGSPEKPTESFRRRSKELPLPDAP 60

Query: 61  RRPGGGEVLRCSSYASFRGSSWSSPISK-RSRLMDPPEEDSSQKLETAVKDEGNNNNNDD 120
              GG EVLRCSS ASF  +SW  P+SK +SRL+DPPEE S  K +           +DD
Sbjct: 61  SNSGGAEVLRCSSNASFSRNSWKPPMSKTKSRLLDPPEE-SCLKSDRVAGSGRALGKDDD 120

Query: 121 DNNNQDDTEDMPEEYDQLKLSPFFIFQLVLSVFVIVGLICNRWVSILKRETIWGLPLWKW 180
           D  +  D ED+PEEY ++K S   +FQ V  VFVI  L+CN W+ I+KR+T+W LPLWKW
Sbjct: 121 DALDDADIEDIPEEYKRIKFSALTLFQWVSLVFVIAALVCNPWIPIIKRQTLWDLPLWKW 180

Query: 181 ELTVLAVICGHLVSILAVRLIVKIIEMNFLLRKRVLYFVYGLRNSVQICLWVSLILLVWH 240
           EL VLA+ICG LVS   +R++V  +E NFLLRKRVLYFVYGLR SVQ CLW+ L+L+VWH
Sbjct: 181 ELLVLALICGRLVSGWGIRVVVFFVERNFLLRKRVLYFVYGLRKSVQNCLWLGLVLVVWH 240

Query: 241 FIFNHKVQIKTQSKILPYVTKIFICFLVGASIWLLKTLAIKILASFFHVNHYFERLKEAL 300
           FIF+ KV+ KTQS+ILPYVTK+ ICFLVG  IWLLKT+ +K+LA  FHVN +FER++EAL
Sbjct: 241 FIFDKKVEEKTQSRILPYVTKVLICFLVGTLIWLLKTILVKVLALSFHVNAFFERIQEAL 300

Query: 301 FSQFVIVSLSGLPLFKRPNTKD------VMMGDQNADG---RE------ESPKSGRVICS 360
           F+Q+VI +LSG PLF+R +T++       +   QNA     RE      +  +SGRVI S
Sbjct: 301 FNQYVIETLSGPPLFERQHTEEEEKVAAEIREFQNAGATMPRELRASLLQRARSGRVIGS 360

Query: 361 GRLVD---CNRSRPLKPDSVKEDEEIPVDQLNKMNQKNISAWNMRRMMNIIRKGALLTLD 420
           GR          R  +P S  +DEEIPVDQL+K+NQKN+SAWNMRRM+NIIR G+L TLD
Sbjct: 361 GRQNSPRVGKSPRVSRPTSGSQDEEIPVDQLHKLNQKNVSAWNMRRMVNIIRHGSLTTLD 420

Query: 421 EKIL-SETNEESLLQIRSERQAKEAAEKIFQRVVQRGSDCIYLEDVMRFMNKEEALMTLS 480
           E+IL S+  ++S L+I++E QAKEAA+KIF +V + G   I LED+M FM+K+EAL T+ 
Sbjct: 421 EQILNSDIEDDSSLKIKTECQAKEAAKKIFLKVAKPGYSYICLEDLMPFMHKDEALKTIH 480

Query: 481 LFGTTAETQCIDESSFREWMVNAFKQRKVLVLSLGDANTAVDDLHSLLNIFVAIIILVIW 540
           LFG   E+  I +S+ R+W+V AF++R+ L LSL D  TAVD+LH++LNI VA+II++IW
Sbjct: 481 LFGAANESDRISKSALRDWVVGAFRERRALALSLNDTKTAVDELHNILNIIVAVIIVIIW 540

Query: 541 LIILGTPVRHFLIFITSQLLLLVFIFGNTCKTVFEAIIFLFVMHPFDIGDRCEVDGVQMI 600
           LIILG PV HFL+ I+SQLLL+VFIFGNTCKTVFEAIIFLFVMHPFD+GDRCEV+GVQM+
Sbjct: 541 LIILGIPVTHFLLLISSQLLLVVFIFGNTCKTVFEAIIFLFVMHPFDVGDRCEVEGVQMV 600

Query: 601 VEEMKILTTVFLRYDNQKITYPNSVLATKAIGNYYRSPDMGEGVDFYVHISTPWEKVALL 660
           VEEM ILTTVFL++D+QKI YPNS+LATK I NY+RSPDMG+ VDF VHISTP EK+A++
Sbjct: 601 VEEMNILTTVFLKFDHQKIIYPNSILATKPIANYHRSPDMGDAVDFCVHISTPLEKLAIM 660

Query: 661 KERITRYVESMSELWHPNPQIVMKEIEDLEKVKMSVCVTHKMNHQDMKERWNRRAVLVEE 690
           KERI  Y+ES S+ W+  P ++M+++EDL K+K+SV  THKMNHQDM  RW RR++L+E 
Sbjct: 661 KERIQGYIESRSDHWYTAPMLIMRDVEDLNKLKISVWPTHKMNHQDMLGRWTRRSLLIEA 720

BLAST of Sed0028199 vs. TAIR 10
Match: AT1G78610.1 (mechanosensitive channel of small conductance-like 6 )

HSP 1 Score: 574.7 bits (1480), Expect = 1.0e-163
Identity = 324/702 (46.15%), Postives = 456/702 (64.96%), Query Frame = 0

Query: 28  KSPQTMRRR---GKEFPEPETRRPGGGEVLRCSSYAS--FRGSSWSSPISKRSRLMDPPE 87
           +SP+   +R   G+      T     GEV++CS   +   R SS    +  RSRL DPP 
Sbjct: 123 ESPRVSTKRDPVGRRDSRSNTNNNDDGEVVKCSGNNAPIQRSSSTLLKMRTRSRLSDPPT 182

Query: 88  ED--------------SSQKLETAVKDEGNNNNNDDDNNNQDDTEDMPEEYDQLKLSPFF 147
                            S ++++    +      +++ ++    ED+PEEY + KLS + 
Sbjct: 183 PQLPPQTADMKSGRIPKSGQMKSGFFGKSPKTQGEEEEDDPFAAEDLPEEYRKDKLSLWI 242

Query: 148 IFQLVLSVFVIVGLICNRWVSILKRETIWGLPLWKWELTVLAVICGHLVSILAVRLIVKI 207
           + + +  + +I G +C   +  L+++ +W L LWKWE  VL +ICG LVS   V+++V  
Sbjct: 243 VLEWLSLILIIAGFVCTLAIPSLRKKKLWELQLWKWESMVLVLICGRLVSSWIVKIVVFF 302

Query: 208 IEMNFLLRKRVLYFVYGLRNSVQICLWVSLILLVWHFIFNHKVQIKTQSKILPYVTKIFI 267
           IE NFLLRKRVLYFVYG+R +VQ CLW+ L+LL WHF+F+ KV     +K L  VTKIF+
Sbjct: 303 IERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLLAWHFLFDEKVAKAANTKALRVVTKIFV 362

Query: 268 CFLVGASIWLLKTLAIKILASFFHVNHYFERLKEALFSQFVIVSLSGLPL---------- 327
           C LVG  +WL+KTL +K+LAS FH++ YF+R++E+LF+Q+VI +LSG PL          
Sbjct: 363 CLLVGFLLWLVKTLLVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLIEIQKNEEEE 422

Query: 328 ---------FKRPNTKDVMMGDQNADGRE-ESPKSGRVICSGRLVDCNRSRPLKPDSVKE 387
                    F+ P   ++  G Q +  +  +SP    V+ +G                 E
Sbjct: 423 ERISVEVKKFQNPGGVEIQSGAQKSPMKTGKSPFLSHVLSNG------------GGGGGE 482

Query: 388 DEEIPVDQLNKMNQKNISAWNMRRMMNIIRKGALLTLDEKILSET-NEESLLQIRSERQA 447
           ++ I +D L+K+N KN+SAW M+R+MNIIR G+L TLDE++   + +++   QIRSE +A
Sbjct: 483 NKGITIDSLHKLNPKNVSAWKMKRLMNIIRNGSLTTLDEQLQDPSLDDDKGNQIRSEFEA 542

Query: 448 KEAAEKIFQRVVQRGSDCIYLEDVMRFMNKEEALMTLSLFGTTAETQCIDESSFREWMVN 507
           K AA KIF  V + GS  IY  D+MRF+  +EAL TLSLF   +ET  I +SS + W+VN
Sbjct: 543 KLAARKIFHNVAKPGSKFIYANDIMRFLPDDEALKTLSLFEGASETNRISKSSLKNWVVN 602

Query: 508 AFKQRKVLVLSLGDANTAVDDLHSLLNIFVAIIILVIWLIILGTPVRHFLIFITSQLLLL 567
           AF++R+ L L+L D  TAV+ LH ++NI V IIILVIWLIILG     FL+ ++SQ++++
Sbjct: 603 AFRERRALALTLNDTKTAVNRLHKMVNIVVGIIILVIWLIILGITSTKFLVVMSSQVVVV 662

Query: 568 VFIFGNTCKTVFEAIIFLFVMHPFDIGDRCEVDGVQMIVEEMKILTTVFLRYDNQKITYP 627
            FIFGN CK VFE+II+LFV+HPFD+GDRCE+DGVQM+VEEM ILTTVFLR+DNQK+ YP
Sbjct: 663 AFIFGNMCKIVFESIIYLFVIHPFDVGDRCEIDGVQMVVEEMNILTTVFLRFDNQKVVYP 722

Query: 628 NSVLATKAIGNYYRSPDMGEGVDFYVHISTPWEKVALLKERITRYVESMSELWHPNPQIV 687
           NS+L TK+IGNYYRSPDMG+G++F +HI+TP EK+ L+K+RIT Y+E   + W+P P IV
Sbjct: 723 NSLLWTKSIGNYYRSPDMGDGIEFSIHITTPAEKIILIKQRITSYIEGKKDHWYPAPMIV 782

Query: 688 MKEIEDLEKVKMSVCVTHKMNHQDMKERWNRRAVLVEEIVTI 690
            K++E L  V+++V  TH+MNHQDM E+W RR+ LVEEI  I
Sbjct: 783 FKDMESLNSVRIAVWPTHRMNHQDMGEKWARRSQLVEEIAKI 812

BLAST of Sed0028199 vs. TAIR 10
Match: AT1G53470.1 (mechanosensitive channel of small conductance-like 4 )

HSP 1 Score: 562.4 bits (1448), Expect = 5.1e-160
Identity = 319/712 (44.80%), Postives = 449/712 (63.06%), Query Frame = 0

Query: 13  SFGNGGDKKIQGLPEKSPQTMRRRGKEFPEPETRRPGGGEVLRCSSYASFRGSSWSSPIS 72
           S+ NG    I+    +   +   +  +  E E  R  G EV++C+S  S   +     + 
Sbjct: 126 SYRNGTGDAIRRRQNRVTLSPSVKDGDSSEDEENRVDGSEVVKCTSNRSTMRTKTLMKMK 185

Query: 73  KRSRLMDPPEEDSSQKLETAVKDEGNNNNNDDDNNNQDDT------------------ED 132
            RSRLMDPP       +       GN N      N +  T                  ED
Sbjct: 186 TRSRLMDPPTPTYPDMVSGRTPRSGNLNPGFSGRNTKPGTPNQGGSKDLEEEEDPFSEED 245

Query: 133 MPEEYDQLKLSPFFIFQLVLSVFVIVGLICNRWVSILKRETIWGLPLWKWELTVLAVICG 192
           +PE   + K+  + I + +  + +I  LIC+  +  L+ +T+W L LWKWE+ VL +ICG
Sbjct: 246 LPEGLRKEKICVWVIIEWIFLILIIASLICSLVIPYLRGKTLWDLALWKWEVMVLVLICG 305

Query: 193 HLVSILAVRLIVKIIEMNFLLRKRVLYFVYGLRNSVQICLWVSLILLVWHFIFNHKVQIK 252
            LVS   V+L V  +E NFL RK+VLYFVYG+R  VQ CLW+ L+L+ WHF+F+ KV+ +
Sbjct: 306 RLVSSWIVKLFVYFVESNFLWRKKVLYFVYGIRKPVQNCLWLGLVLIAWHFLFDKKVERE 365

Query: 253 TQSKILPYVTKIFICFLVGASIWLLKTLAIKILASFFHVNHYFERLKEALFSQFVIVSLS 312
            +S +L YVTK+ IC LV   IWL+KTL +K+LAS FH++ YF+R++E+LF+Q+VI +LS
Sbjct: 366 MRSTVLKYVTKVLICLLVAVIIWLIKTLLVKVLASSFHMSTYFDRIQESLFTQYVIETLS 425

Query: 313 GLPLFKRPNTKDVMMGDQNA---DGREESPKSGRVICSGRLVDCNRSRPLK--------- 372
           G P  +    ++ +  D       GR+ SP   + + S   V     R  K         
Sbjct: 426 GPPRIEIHIEEEKVANDVKTFEIVGRKLSPLGPKAVSSPPQVTVGSGRLQKSPSRVGKSP 485

Query: 373 ----PDSVKE--DEEIPVDQLNKMNQKNISAWNMRRMMNIIRKGALLTLDEKILSETNEE 432
                 S KE  +E I +D L +MN KN+SAW M+++MN+I+KG L TLDE+I   T +E
Sbjct: 486 VLSRSGSKKEGGEEGIRIDHLQRMNTKNVSAWKMKKLMNVIKKGTLSTLDEQIQDTTTQE 545

Query: 433 --SLLQIRSERQAKEAAEKIFQRVVQRGSDCIYLEDVMRFMNKEEALMTLSLFGTTAETQ 492
                QIRSE +AK AA KIFQ V + GS  IY+ED MRF++++E+   + LF   +E  
Sbjct: 546 DDKATQIRSEFEAKLAARKIFQNVAEPGSRYIYMEDFMRFLSEDESERAMDLFEGASECH 605

Query: 493 CIDESSFREWMVNAFKQRKVLVLSLGDANTAVDDLHSLLNIFVAIIILVIWLIILGTPVR 552
            I +S  + W+VNAF++R+ L L+L D  TAV+ LH ++++ V+I+IL+IWL+ILG    
Sbjct: 606 KISKSCLKNWVVNAFRERRALALTLNDTKTAVNRLHRIVDVLVSIVILIIWLLILGIATT 665

Query: 553 HFLIFITSQLLLLVFIFGNTCKTVFEAIIFLFVMHPFDIGDRCEVDGVQMIVEEMKILTT 612
            FL+ I+SQLLL+VF+FGN+CKT+FEA+IF+FVMHPFD+GDRCE+DGVQMIVEEM ILTT
Sbjct: 666 KFLLVISSQLLLVVFVFGNSCKTIFEAVIFVFVMHPFDVGDRCEIDGVQMIVEEMNILTT 725

Query: 613 VFLRYDNQKITYPNSVLATKAIGNYYRSPDMGEGVDFYVHISTPWEKVALLKERITRYVE 672
           VFLR+DNQKI YPNS+L TK I NYYRSPDM + ++F+VHI+TP EK   L++RI  YV+
Sbjct: 726 VFLRFDNQKIVYPNSLLGTKPIANYYRSPDMQDAIEFFVHIATPPEKTTALRQRILSYVD 785

Query: 673 SMSELWHPNPQIVMKEIEDLEKVKMSVCVTHKMNHQDMKERWNRRAVLVEEI 687
           +  + WHP+P IV +++  L  VK+++  THKMNHQ+M ER+ RR  L+EEI
Sbjct: 786 NKKDHWHPSPMIVFRDMCGLNSVKIAMWPTHKMNHQNMGERYVRRGQLLEEI 837

BLAST of Sed0028199 vs. TAIR 10
Match: AT3G14810.1 (mechanosensitive channel of small conductance-like 5 )

HSP 1 Score: 561.6 bits (1446), Expect = 8.7e-160
Identity = 315/680 (46.32%), Postives = 438/680 (64.41%), Query Frame = 0

Query: 42  EPETRRPGGGEVLRCSSYASFRGSSWSSPISKRSRLMDPPEEDSSQKLETAVKD------ 101
           + E R     EVL+C S       +       +SRL DPP        +T +K       
Sbjct: 168 DEEGRNRDEAEVLKCGSKKPMLSRN-----KTKSRLQDPPTPTHPAIDKTEMKSGRRSGI 227

Query: 102 --------------EGNNNNNDDDNNNQDDTEDMPEEYDQLKLSPFFIFQLVLSVFVIVG 161
                          G N   +++  +    ED+PEE+ + KLS +   + +  V ++  
Sbjct: 228 FKSGFLGKSPKAGTPGRNGFEEEEEEDPFLDEDLPEEFKRDKLSFWVFLEWISLVLIVTS 287

Query: 162 LICNRWVSILKRETIWGLPLWKWELTVLAVICGHLVSILAVRLIVKIIEMNFLLRKRVLY 221
           L+C+  +  L+R+T W L LWKWE+TVL +ICG LVS   VR+IV ++E NF  RKRVLY
Sbjct: 288 LVCSLTIHNLQRKTWWKLDLWKWEVTVLVLICGRLVSSWIVRIIVFLVEKNFTWRKRVLY 347

Query: 222 FVYGLRNSVQICLWVSLILLVWHFIFNHKVQIKTQSKILPYVTKIFICFLVGASIWLLKT 281
           FVYG+R SVQ CLW+ L+LL WHF+F+ KV+ +T+S  L YVT++ +C LV   IWL+KT
Sbjct: 348 FVYGVRKSVQNCLWLGLVLLAWHFLFDKKVERETRSTALRYVTRVLVCLLVALIIWLVKT 407

Query: 282 LAIKILASFFHVNHYFERLKEALFSQFVIVSLSGLPLFKRPNTKDVMMGDQNADGREESP 341
           + +K+LAS FH++ YF+R++E+LF+Q+VI +LSG PL       ++   ++      E  
Sbjct: 408 ILVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLM------EIQRMEEEEQQVAEDV 467

Query: 342 KSGRVICSGRL-----------VDCNRSRPLKPDSVKEDEE---IPVDQLNKMNQKNISA 401
           KS   +   +L           +   +S  L     K  E+   I +DQL +MN KN+SA
Sbjct: 468 KSLEKLAGAKLPPALKATVKSFMKVGKSPGLNRIGSKRGEDGEGIRIDQLKRMNTKNVSA 527

Query: 402 WNMRRMMNIIRKGALLTLDEKILSETNE-ESLLQIRSERQAKEAAEKIFQRVVQRGSDCI 461
           WNM+R+MNII KGA+ TLD+ +   T E E    IRSE +AK AA KIF  V + GS  I
Sbjct: 528 WNMKRLMNIILKGAISTLDQNMQDTTQEDEDATHIRSEYEAKCAARKIFHNVTEPGSRYI 587

Query: 462 YLEDVMRFMNKEEALMTLSLFGTTAETQCIDESSFREWMVNAFKQRKVLVLSLGDANTAV 521
           YLED +RF+ +EEA   ++LF   +E+  I +S  + W+V AF++R+ L L+L D  TAV
Sbjct: 588 YLEDFLRFLCEEEAERAMALFEGASESDKISKSCLKNWVVKAFRERRALALTLNDTKTAV 647

Query: 522 DDLHSLLNIFVAIIILVIWLIILGTPVRHFLIFITSQLLLLVFIFGNTCKTVFEAIIFLF 581
           D LH ++N+ + III++IWL+ILG     FL+ ++SQLLL+ F+FGN+CKT+FEAIIFLF
Sbjct: 648 DRLHRIINVVIGIIIIIIWLLILGIATTRFLLVLSSQLLLVAFVFGNSCKTIFEAIIFLF 707

Query: 582 VMHPFDIGDRCEVDGVQMIVEEMKILTTVFLRYDNQKITYPNSVLATKAIGNYYRSPDMG 641
           VMHPFD+GDRCE+DGVQ++VEEM ILTTVFLRYDNQKI YPNSVL TK I NYYRSPDMG
Sbjct: 708 VMHPFDVGDRCEIDGVQLVVEEMNILTTVFLRYDNQKIIYPNSVLGTKPIANYYRSPDMG 767

Query: 642 EGVDFYVHISTPWEKVALLKERITRYVESMSELWHPNPQIVMKEIEDLEKVKMSVCVTHK 687
           + V+F VHI+TP EK+  +K+RI  YV++  + W+P P IV   ++DL  VK++V +TH+
Sbjct: 768 DAVEFCVHIATPPEKITAIKQRILSYVDNKKDYWYPAPMIVFLSMDDLNSVKIAVWLTHR 827

BLAST of Sed0028199 vs. TAIR 10
Match: AT2G17000.1 (Mechanosensitive ion channel family protein )

HSP 1 Score: 545.8 bits (1405), Expect = 5.0e-155
Identity = 324/716 (45.25%), Postives = 467/716 (65.22%), Query Frame = 0

Query: 3   SKE-EVFLKIDSFG----NGGDKKIQGLPEKSPQTMRRRGKEFPEPETRRPGGGEVLRCS 62
           SKE  VF KI+S G    +G  +        S  TMR   ++  E E     G  V+RCS
Sbjct: 104 SKETRVFFKINSSGTNNMSGSVRSCTSSTSFSSATMRLNLEQQLEDE-----GEVVVRCS 163

Query: 63  SYASFRGSSWSSPISKRSRLMDPPEEDSSQKLE-TAVKDE---GNNNNNDDDNNNQDDT- 122
              S R +   S    RSRL+DPP+E+  Q        D+   G    + DD   +DD+ 
Sbjct: 164 ---SVRKTELVSRAKARSRLIDPPQEEEQQYSSWIGTSDQLRSGLLGRHSDDIEEEDDSS 223

Query: 123 --EDMPEEYDQLKLSPFFIFQLVLSVFVIVGLICNRWVSILKRETIWGLPLWKWELTVLA 182
             ED+P EY +LK+    + Q +  + ++V L+ +  +   +  T+W L LWKWE+ +L 
Sbjct: 224 AEEDVPVEYRKLKMDAITLLQWMSLIALVVALVLSLGLHTWRNATLWSLHLWKWEVVLLV 283

Query: 183 VICGHLVSILAVRLIVKIIEMNFLLRKRVLYFVYGLRNSVQICLWVSLILLVWHFIFNHK 242
           +ICG LVS   +R+IV  IE NFLLRKRVLYFVYG++ +VQ CLW+ L+LL WHF+F+ K
Sbjct: 284 LICGRLVSGCGIRIIVFFIERNFLLRKRVLYFVYGVKTAVQNCLWLGLVLLAWHFLFDKK 343

Query: 243 VQIKTQSKILPYVTKIFICFLVGASIWLLKTLAIKILASFFHVNHYFERLKEALFSQFVI 302
           V+ +TQS +L  ++KI +CFL+   +WL+KTL +K+LAS FHV+ YF+R++EALF  ++I
Sbjct: 344 VEKETQSDVLLLMSKILVCFLLSTVLWLIKTLVVKVLASSFHVSTYFDRIQEALFHHYLI 403

Query: 303 VSLSGLPLF-------KRPNTKDVMMGDQNADGREESPKSGRVIC--------SGRLVDC 362
            +LSG P+        +   T+D +   Q   G + SP+    +C        SG  ++ 
Sbjct: 404 ETLSGPPMLELSRIEEEEDRTQDEIYKMQKG-GADLSPE----LCSAAFPQEKSGSTMNM 463

Query: 363 NRSRPLKPDSVKEDEEIPVDQLNKMNQKNISAWNMRRMMNIIRKGALLTLDEKILSET-N 422
             S P+ P     D  I +D L+KMNQKN+SAWNM+R+M I+R  +L TLDE+ L  T  
Sbjct: 464 KFS-PIIP-KTGSDNGITMDDLHKMNQKNVSAWNMKRLMKIVRNVSLSTLDEQALQNTCE 523

Query: 423 EESLLQIRSERQAKEAAEKIFQRVVQRGSDCIYLEDVMRFMNKEEALMTLSLFGTTAETQ 482
           +ES  QIRSE++AK AA KIF+ V Q G+  IYLED+MRF+  +EA+ T+ LF     T+
Sbjct: 524 DESTRQIRSEKEAKAAARKIFKNVAQPGTKHIYLEDLMRFLRVDEAMKTMCLFEGALVTK 583

Query: 483 CIDESSFREWMVNAFKQRKVLVLSLGDANTAVDDLHSLLNIFVAIIILVIWLIILGTPVR 542
            I +S+ + W+VNAF++R+ L L+L D  TAV+ LH +++   AI+I+VIWLI+L     
Sbjct: 584 KITKSALKNWLVNAFRERRALALTLNDTKTAVNKLHHMISFLTAIVIIVIWLILLEIATS 643

Query: 543 HFLIFITSQLLLLVFIFGNTCKTVFEAIIFLFVMHPFDIGDRCEVDGVQMIVEEMKILTT 602
            +L+F+TSQ++LL F+FGN+ KTVFE+IIFLF++HP+D+GDR  +D V+M+VEEM ILTT
Sbjct: 644 KYLLFLTSQVVLLAFMFGNSLKTVFESIIFLFIIHPYDVGDRLLIDTVEMVVEEMNILTT 703

Query: 603 VFLRYDNQKITYPNSVLATKAIGNYYRSPDMGEGVDFYVHISTPWEKVALLKERITRYVE 662
           VFLR DN KI YPN +L  KAI NY RSPDMG+ V   VHI+TP EK+A +K+RI+ Y++
Sbjct: 704 VFLRADNLKIVYPNILLWQKAIHNYNRSPDMGDEVTCCVHITTPPEKIAAIKQRISSYID 763

Query: 663 SMSELWHPNPQIVMKEIEDLEKVKMSVCVTHKMNHQDMKERWNRRAVLVEEIVTIL 691
           S  E W+P   +++K++EDL  V++++ + HK+NHQ+M ER+ RRA+L+EE++ IL
Sbjct: 764 SKPEYWYPKADVIVKDVEDLNIVRIAIWLCHKINHQNMGERFTRRALLIEEVIKIL 804

BLAST of Sed0028199 vs. TAIR 10
Match: AT3G14810.2 (mechanosensitive channel of small conductance-like 5 )

HSP 1 Score: 501.9 bits (1291), Expect = 8.2e-142
Identity = 297/680 (43.68%), Postives = 411/680 (60.44%), Query Frame = 0

Query: 42  EPETRRPGGGEVLRCSSYASFRGSSWSSPISKRSRLMDPPEEDSSQKLETAVKD------ 101
           + E R     EVL+C S       +       +SRL DPP        +T +K       
Sbjct: 168 DEEGRNRDEAEVLKCGSKKPMLSRN-----KTKSRLQDPPTPTHPAIDKTEMKSGRRSGI 227

Query: 102 --------------EGNNNNNDDDNNNQDDTEDMPEEYDQLKLSPFFIFQLVLSVFVIVG 161
                          G N   +++  +    ED+PEE+ + KLS +   + +  V ++  
Sbjct: 228 FKSGFLGKSPKAGTPGRNGFEEEEEEDPFLDEDLPEEFKRDKLSFWVFLEWISLVLIVTS 287

Query: 162 LICNRWVSILKRETIWGLPLWKWELTVLAVICGHLVSILAVRLIVKIIEMNFLLRKRVLY 221
           L+C+  +  L+R+T W L LWKWE+TVL +ICG LVS   VR+IV ++E NF  RKRVLY
Sbjct: 288 LVCSLTIHNLQRKTWWKLDLWKWEVTVLVLICGRLVSSWIVRIIVFLVEKNFTWRKRVLY 347

Query: 222 FVYGLRNSVQICLWVSLILLVWHFIFNHKVQIKTQSKILPYVTKIFICFLVGASIWLLKT 281
           FVYG+R SVQ CLW+ L+LL WHF+F+ KV+ +T+S  L                     
Sbjct: 348 FVYGVRKSVQNCLWLGLVLLAWHFLFDKKVERETRSTAL--------------------- 407

Query: 282 LAIKILASFFHVNHYFERLKEALFSQFVIVSLSGLPLFKRPNTKDVMMGDQNADGREESP 341
                         YF+R++E+LF+Q+VI +LSG PL       ++   ++      E  
Sbjct: 408 ------------RTYFDRIQESLFTQYVIETLSGPPLM------EIQRMEEEEQQVAEDV 467

Query: 342 KSGRVICSGRL-----------VDCNRSRPLKPDSVKEDEE---IPVDQLNKMNQKNISA 401
           KS   +   +L           +   +S  L     K  E+   I +DQL +MN KN+SA
Sbjct: 468 KSLEKLAGAKLPPALKATVKSFMKVGKSPGLNRIGSKRGEDGEGIRIDQLKRMNTKNVSA 527

Query: 402 WNMRRMMNIIRKGALLTLDEKILSETNE-ESLLQIRSERQAKEAAEKIFQRVVQRGSDCI 461
           WNM+R+MNII KGA+ TLD+ +   T E E    IRSE +AK AA KIF  V + GS  I
Sbjct: 528 WNMKRLMNIILKGAISTLDQNMQDTTQEDEDATHIRSEYEAKCAARKIFHNVTEPGSRYI 587

Query: 462 YLEDVMRFMNKEEALMTLSLFGTTAETQCIDESSFREWMVNAFKQRKVLVLSLGDANTAV 521
           YLED +RF+ +EEA   ++LF   +E+  I +S  + W+  AF++R+ L L+L D  TAV
Sbjct: 588 YLEDFLRFLCEEEAERAMALFEGASESDKISKSCLKNWV--AFRERRALALTLNDTKTAV 647

Query: 522 DDLHSLLNIFVAIIILVIWLIILGTPVRHFLIFITSQLLLLVFIFGNTCKTVFEAIIFLF 581
           D LH ++N+ + III++IWL+ILG     FL+ ++SQLLL+ F+FGN+CKT+FEAIIFLF
Sbjct: 648 DRLHRIINVVIGIIIIIIWLLILGIATTRFLLVLSSQLLLVAFVFGNSCKTIFEAIIFLF 707

Query: 582 VMHPFDIGDRCEVDGVQMIVEEMKILTTVFLRYDNQKITYPNSVLATKAIGNYYRSPDMG 641
           VMHPFD+GDRCE+DGVQ++VEEM ILTTVFLRYDNQKI YPNSVL TK I NYYRSPDMG
Sbjct: 708 VMHPFDVGDRCEIDGVQLVVEEMNILTTVFLRYDNQKIIYPNSVLGTKPIANYYRSPDMG 767

Query: 642 EGVDFYVHISTPWEKVALLKERITRYVESMSELWHPNPQIVMKEIEDLEKVKMSVCVTHK 687
           + V+F VHI+TP EK+  +K+RI  YV++  + W+P P IV   ++DL  VK++V +TH+
Sbjct: 768 DAVEFCVHIATPPEKITAIKQRILSYVDNKKDYWYPAPMIVFLSMDDLNSVKIAVWLTHR 801

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022936287.10.0e+0081.88mechanosensitive ion channel protein 8-like [Cucurbita moschata][more]
XP_022975440.10.0e+0081.74mechanosensitive ion channel protein 8-like [Cucurbita maxima][more]
XP_023535846.10.0e+0081.46mechanosensitive ion channel protein 8-like isoform X1 [Cucurbita pepo subsp. pe... [more]
XP_022140870.12.6e-30779.80mechanosensitive ion channel protein 6-like isoform X1 [Momordica charantia][more]
XP_023535848.13.2e-29777.03mechanosensitive ion channel protein 8-like isoform X2 [Cucurbita pepo subsp. pe... [more]
Match NameE-valueIdentityDescription
F4IME21.6e-16649.02Mechanosensitive ion channel protein 8 OS=Arabidopsis thaliana OX=3702 GN=MSL8 P... [more]
Q9SYM11.4e-16246.15Mechanosensitive ion channel protein 6 OS=Arabidopsis thaliana OX=3702 GN=MSL6 P... [more]
Q9LPG37.2e-15944.80Mechanosensitive ion channel protein 4 OS=Arabidopsis thaliana OX=3702 GN=MSL4 P... [more]
Q9LH741.2e-15846.32Mechanosensitive ion channel protein 5 OS=Arabidopsis thaliana OX=3702 GN=MSL5 P... [more]
F4IME17.0e-15445.25Mechanosensitive ion channel protein 7 OS=Arabidopsis thaliana OX=3702 GN=MSL7 P... [more]
Match NameE-valueIdentityDescription
A0A6J1F8110.0e+0081.88Mechanosensitive ion channel protein OS=Cucurbita moschata OX=3662 GN=LOC1114429... [more]
A0A6J1IGQ20.0e+0081.74Mechanosensitive ion channel protein OS=Cucurbita maxima OX=3661 GN=LOC111474765... [more]
A0A6J1CGD41.3e-30779.80Mechanosensitive ion channel protein OS=Momordica charantia OX=3673 GN=LOC111011... [more]
A0A6J1CH061.8e-28575.36Mechanosensitive ion channel protein OS=Momordica charantia OX=3673 GN=LOC111011... [more]
A0A5E4FCX01.5e-21257.18Mechanosensitive ion channel protein OS=Prunus dulcis OX=3755 GN=ALMOND_2B014830... [more]
Match NameE-valueIdentityDescription
AT1G78610.11.0e-16346.15mechanosensitive channel of small conductance-like 6 [more]
AT1G53470.15.1e-16044.80mechanosensitive channel of small conductance-like 4 [more]
AT3G14810.18.7e-16046.32mechanosensitive channel of small conductance-like 5 [more]
AT2G17000.15.0e-15545.25Mechanosensitive ion channel family protein [more]
AT3G14810.28.2e-14243.68mechanosensitive channel of small conductance-like 5 [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR016688Mechanosensitive ion channel MscS-like, plants/fungiPIRSFPIRSF017209Memb_At2g17000coord: 21..693
e-value: 7.0E-198
score: 657.8
IPR016688Mechanosensitive ion channel MscS-like, plants/fungiPANTHERPTHR31618MECHANOSENSITIVE ION CHANNEL PROTEIN 5coord: 4..691
IPR006685Mechanosensitive ion channel MscSPFAMPF00924MS_channelcoord: 492..691
e-value: 1.5E-20
score: 73.6
IPR023408Mechanosensitive ion channel MscS domain superfamilyGENE3D2.30.30.60coord: 550..599
e-value: 3.8E-12
score: 48.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..116
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 31..49
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 76..101
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 59..73
NoneNo IPR availablePANTHERPTHR31618:SF8MECHANOSENSITIVE ION CHANNEL PROTEINcoord: 4..691
IPR010920LSM domain superfamilySUPERFAMILY50182Sm-like ribonucleoproteinscoord: 540..599

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0028199.1Sed0028199.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006820 anion transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0008381 mechanosensitive ion channel activity