Sed0028023 (gene) Chayote v1

Overview


Name	Sed0028023
Type	gene
Organism	Sechium edule (Chayote v1)
Description	Cupin_3 domain-containing protein
Location	LG09: 25102952 .. 25103923 (+)
RNA-Seq Expression	Sed0028023
Synteny	Sed0028023

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AGTCGCTATAAATTCTCTCACAAACTTCATCATTCTCCTCATTTTTGTTCCATCCAAAACCATAAGATTCCTTTCCTTTCATTGTCTTTTATTTCCCTTTCTCTTTCTGTCTCTCTTTCTCTCAATAAATACCTCCACATAAATCCCACCCTTCTCTTTTAATTTCCCCTCACCCTTTTTGTAACCTAAAGGAAAAGAAGAATATGGCCAATGAAGAGGGAAATAGCAACAATCCTAACTCAACCAACAACCTTAGAATCATTGTAGAAAGAAACCCTTCTCAAGCCAAGCTCTCTCAGCTCAACATCCACTGCTGGCCAAAGTAAGTATACCACGATTCCGTCTATCGATTTAAGCTTTTGAGTTCGGTTCCATAATAATTGCTTAACTTTTTTAGATGGGGTTGTTCCAAACACACTATAATCCATCCATTTAAACTTTTGGGTTGGGTATGCTGTCACTTATTAGTTTAAGCTTTTGAGCTCTCGATCGGGTGATAATTAGCTGCTTTTATTTACATGGGGTTGTTTGAAATAATCTATCCACTTAAACTTTTGTGTTCGATGGGATATGATTTAGAACACTCTAATCTATCGGTTTAAGCTTTCGTGTGTTAGATGGGGGTGTTCGGCGGGGAAATACCAACTGAAATTTGAGGCGGAAGAGACATGCTATTTGGTGAAGGGAAAAGTGAAGGCTTATGCAAAAGGATCAGCTGATTCTGAATACACTGAGTTTGGGGCTGGCGATTTGGTCACTATTCCTAAAGGACTTAGCTGCACTTGGGATGTTTCTGTTGCTGTTGATAAGTTCTACAAGTTTGAGTCTCAATCTTAAATCCAATCTTTTCTTTTTATTTTCTTATAATTAGTCCCTCTAATTTACCTTCTATTTCTTTCTTTCCCTTTTCTTTTTCTGTAAATATATTCTTCTTTTCCTCTATCTCTAAAATGGGAGAGAGAAGGGTTGGAG

mRNA sequence

AGTCGCTATAAATTCTCTCACAAACTTCATCATTCTCCTCATTTTTGTTCCATCCAAAACCATAAGATTCCTTTCCTTTCATTGTCTTTTATTTCCCTTTCTCTTTCTGTCTCTCTTTCTCTCAATAAATACCTCCACATAAATCCCACCCTTCTCTTTTAATTTCCCCTCACCCTTTTTGTAACCTAAAGGAAAAGAAGAATATGGCCAATGAAGAGGGAAATAGCAACAATCCTAACTCAACCAACAACCTTAGAATCATTGTAGAAAGAAACCCTTCTCAAGCCAAGCTCTCTCAGCTCAACATCCACTGCTGGCCAAAATGGGGGTGTTCGGCGGGGAAATACCAACTGAAATTTGAGGCGGAAGAGACATGCTATTTGGTGAAGGGAAAAGTGAAGGCTTATGCAAAAGGATCAGCTGATTCTGAATACACTGAGTTTGGGGCTGGCGATTTGGTCACTATTCCTAAAGGACTTAGCTGCACTTGGGATGTTTCTGTTGCTGTTGATAAGTTCTACAAGTTTGAGTCTCAATCTTAAATCCAATCTTTTCTTTTTATTTTCTTATAATTAGTCCCTCTAATTTACCTTCTATTTCTTTCTTTCCCTTTTCTTTTTCTGTAAATATATTCTTCTTTTCCTCTATCTCTAAAATGGGAGAGAGAAGGGTTGGAG

Coding sequence (CDS)

ATGGCCAATGAAGAGGGAAATAGCAACAATCCTAACTCAACCAACAACCTTAGAATCATTGTAGAAAGAAACCCTTCTCAAGCCAAGCTCTCTCAGCTCAACATCCACTGCTGGCCAAAATGGGGGTGTTCGGCGGGGAAATACCAACTGAAATTTGAGGCGGAAGAGACATGCTATTTGGTGAAGGGAAAAGTGAAGGCTTATGCAAAAGGATCAGCTGATTCTGAATACACTGAGTTTGGGGCTGGCGATTTGGTCACTATTCCTAAAGGACTTAGCTGCACTTGGGATGTTTCTGTTGCTGTTGATAAGTTCTACAAGTTTGAGTCTCAATCTTAA

Protein sequence

MANEEGNSNNPNSTNNLRIIVERNPSQAKLSQLNIHCWPKWGCSAGKYQLKFEAEETCYLVKGKVKAYAKGSADSEYTEFGAGDLVTIPKGLSCTWDVSVAVDKFYKFESQS

Homology

[Close]

BLAST of Sed0028023 vs. NCBI nr
Match: KAG7025258.1 (hypothetical protein SDJN02_11753 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 213.4 bits (542), Expect = 1.0e-51
Identity = 105/112 (93.75%), Postives = 107/112 (95.54%), Query Frame = 0


Query: 1   MANEEGNSNNPNSTNNLRIIVERNPSQAKLSQLNIHCWPKWGCSAGKYQLKFEAEETCYL 60
           MANEEGNSNNP STNNLRIIVERNPSQAKLS LNI  WPKWGCSAGKYQLKFEAEETCYL
Sbjct: 1   MANEEGNSNNP-STNNLRIIVERNPSQAKLSMLNIQSWPKWGCSAGKYQLKFEAEETCYL 60

Query: 61  VKGKVKAYAKGSADSEYTEFGAGDLVTIPKGLSCTWDVSVAVDKFYKFESQS 113
           VKGKVKAYAKGSA++EYTEFGAGDLVTIPKGLSCTWDVSVAVDKFYKFES S
Sbjct: 61  VKGKVKAYAKGSAETEYTEFGAGDLVTIPKGLSCTWDVSVAVDKFYKFESPS 111

[Close]

BLAST of Sed0028023 vs. NCBI nr
Match: KAG6592849.1 (hypothetical protein SDJN03_12325, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 213.0 bits (541), Expect = 1.3e-51
Identity = 104/112 (92.86%), Postives = 107/112 (95.54%), Query Frame = 0


Query: 1   MANEEGNSNNPNSTNNLRIIVERNPSQAKLSQLNIHCWPKWGCSAGKYQLKFEAEETCYL 60
           MANEEGNSNNP STNNLRII+ERNPSQAKLS LNI  WPKWGCSAGKYQLKFEAEETCYL
Sbjct: 1   MANEEGNSNNP-STNNLRIIIERNPSQAKLSMLNIQSWPKWGCSAGKYQLKFEAEETCYL 60

Query: 61  VKGKVKAYAKGSADSEYTEFGAGDLVTIPKGLSCTWDVSVAVDKFYKFESQS 113
           VKGKVKAYAKGSA++EYTEFGAGDLVTIPKGLSCTWDVSVAVDKFYKFES S
Sbjct: 61  VKGKVKAYAKGSAETEYTEFGAGDLVTIPKGLSCTWDVSVAVDKFYKFESPS 111

[Close]

BLAST of Sed0028023 vs. NCBI nr
Match: XP_022960185.1 (uncharacterized protein LOC111460998 [Cucurbita moschata] >XP_022960186.1 uncharacterized protein LOC111460998 [Cucurbita moschata] >XP_023513548.1 uncharacterized protein LOC111778119 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 212.6 bits (540), Expect = 1.7e-51
Identity = 105/112 (93.75%), Postives = 107/112 (95.54%), Query Frame = 0


Query: 1   MANEEGNSNNPNSTNNLRIIVERNPSQAKLSQLNIHCWPKWGCSAGKYQLKFEAEETCYL 60
           MANEEGNSNNP STNNLRIIVERNPSQAKLS LNI  WPKWGCSAGKYQLKFEAEETCYL
Sbjct: 1   MANEEGNSNNP-STNNLRIIVERNPSQAKLSLLNIQSWPKWGCSAGKYQLKFEAEETCYL 60

Query: 61  VKGKVKAYAKGSADSEYTEFGAGDLVTIPKGLSCTWDVSVAVDKFYKFESQS 113
           VKGKVKAYAKGSA++EYTEFGAGDLVTIPKGLSCTWDVSVAVDKFYKFES S
Sbjct: 61  VKGKVKAYAKGSAETEYTEFGAGDLVTIPKGLSCTWDVSVAVDKFYKFESPS 111

[Close]

BLAST of Sed0028023 vs. NCBI nr
Match: XP_023005099.1 (uncharacterized protein LOC111498188 [Cucurbita maxima] >XP_023005100.1 uncharacterized protein LOC111498188 [Cucurbita maxima] >XP_023005101.1 uncharacterized protein LOC111498188 [Cucurbita maxima])

HSP 1 Score: 211.5 bits (537), Expect = 3.8e-51
Identity = 104/112 (92.86%), Postives = 107/112 (95.54%), Query Frame = 0


Query: 1   MANEEGNSNNPNSTNNLRIIVERNPSQAKLSQLNIHCWPKWGCSAGKYQLKFEAEETCYL 60
           MANEEGNSNNP STNNLRIIVERNPS+AKLS LNI  WPKWGCSAGKYQLKFEAEETCYL
Sbjct: 1   MANEEGNSNNP-STNNLRIIVERNPSEAKLSLLNIQSWPKWGCSAGKYQLKFEAEETCYL 60

Query: 61  VKGKVKAYAKGSADSEYTEFGAGDLVTIPKGLSCTWDVSVAVDKFYKFESQS 113
           VKGKVKAYAKGSA++EYTEFGAGDLVTIPKGLSCTWDVSVAVDKFYKFES S
Sbjct: 61  VKGKVKAYAKGSAETEYTEFGAGDLVTIPKGLSCTWDVSVAVDKFYKFESPS 111

[Close]

BLAST of Sed0028023 vs. NCBI nr
Match: XP_038906875.1 (uncharacterized protein LOC120092760 [Benincasa hispida])

HSP 1 Score: 195.7 bits (496), Expect = 2.2e-46
Identity = 97/116 (83.62%), Postives = 102/116 (87.93%), Query Frame = 0


Query: 3   NEEGNSNNPNSTNNLRIIVERNPSQAKLSQLNIHCWPKWGCSAGKYQLKFEAEETCYLVK 62
           NEE  + NP++ NNL+IIVERNPSQAKLSQLNIH WPKWGCSAGKYQLKFEAEETCYLVK
Sbjct: 4   NEEEGNKNPSTNNNLKIIVERNPSQAKLSQLNIHGWPKWGCSAGKYQLKFEAEETCYLVK 63

Query: 63  GKVKAYAKG------SADSEYTEFGAGDLVTIPKGLSCTWDVSVAVDKFYKFESQS 113
           GKVKAY KG      S+  EYTEFGAGDLVTIPKGLSCTWDVSVAVDKFYKFESQS
Sbjct: 64  GKVKAYPKGIDSSSSSSCEEYTEFGAGDLVTIPKGLSCTWDVSVAVDKFYKFESQS 119

[Close]

BLAST of Sed0028023 vs. ExPASy TrEMBL
Match: A0A6J1H6X6 (uncharacterized protein LOC111460998 OS=Cucurbita moschata OX=3662 GN=LOC111460998 PE=4 SV=1)

HSP 1 Score: 212.6 bits (540), Expect = 8.4e-52
Identity = 105/112 (93.75%), Postives = 107/112 (95.54%), Query Frame = 0


Query: 1   MANEEGNSNNPNSTNNLRIIVERNPSQAKLSQLNIHCWPKWGCSAGKYQLKFEAEETCYL 60
           MANEEGNSNNP STNNLRIIVERNPSQAKLS LNI  WPKWGCSAGKYQLKFEAEETCYL
Sbjct: 1   MANEEGNSNNP-STNNLRIIVERNPSQAKLSLLNIQSWPKWGCSAGKYQLKFEAEETCYL 60

Query: 61  VKGKVKAYAKGSADSEYTEFGAGDLVTIPKGLSCTWDVSVAVDKFYKFESQS 113
           VKGKVKAYAKGSA++EYTEFGAGDLVTIPKGLSCTWDVSVAVDKFYKFES S
Sbjct: 61  VKGKVKAYAKGSAETEYTEFGAGDLVTIPKGLSCTWDVSVAVDKFYKFESPS 111

[Close]

BLAST of Sed0028023 vs. ExPASy TrEMBL
Match: A0A6J1KU03 (uncharacterized protein LOC111498188 OS=Cucurbita maxima OX=3661 GN=LOC111498188 PE=4 SV=1)

HSP 1 Score: 211.5 bits (537), Expect = 1.9e-51
Identity = 104/112 (92.86%), Postives = 107/112 (95.54%), Query Frame = 0


Query: 1   MANEEGNSNNPNSTNNLRIIVERNPSQAKLSQLNIHCWPKWGCSAGKYQLKFEAEETCYL 60
           MANEEGNSNNP STNNLRIIVERNPS+AKLS LNI  WPKWGCSAGKYQLKFEAEETCYL
Sbjct: 1   MANEEGNSNNP-STNNLRIIVERNPSEAKLSLLNIQSWPKWGCSAGKYQLKFEAEETCYL 60

Query: 61  VKGKVKAYAKGSADSEYTEFGAGDLVTIPKGLSCTWDVSVAVDKFYKFESQS 113
           VKGKVKAYAKGSA++EYTEFGAGDLVTIPKGLSCTWDVSVAVDKFYKFES S
Sbjct: 61  VKGKVKAYAKGSAETEYTEFGAGDLVTIPKGLSCTWDVSVAVDKFYKFESPS 111

[Close]

BLAST of Sed0028023 vs. ExPASy TrEMBL
Match: A0A1S3CBJ5 (uncharacterized protein LOC103498932 OS=Cucumis melo OX=3656 GN=LOC103498932 PE=4 SV=1)

HSP 1 Score: 192.6 bits (488), Expect = 8.9e-46
Identity = 99/125 (79.20%), Postives = 103/125 (82.40%), Query Frame = 0


Query: 1   MANEEG----NSNNPNSTNNLRIIVERNPSQAKLSQLNIHCWPKWGCSAGKYQLKFEAEE 60
           MANEEG    N NNP++ N+L+IIVERNPSQAKLSQLNIH WPKWGCSAGKYQLKFEAEE
Sbjct: 1   MANEEGGNNNNHNNPSTNNSLKIIVERNPSQAKLSQLNIHRWPKWGCSAGKYQLKFEAEE 60

Query: 61  TCYLVKGKVKAYAKG---------SADSEYTEFGAGDLVTIPKGLSCTWDVSVAVDKFYK 113
           TCYLVKGKVKAY KG         S   EY EFGAGDLV IPKGLSCTWDVSVAVDKFYK
Sbjct: 61  TCYLVKGKVKAYPKGIDSSSSSSSSCCEEYIEFGAGDLVIIPKGLSCTWDVSVAVDKFYK 120

[Close]

BLAST of Sed0028023 vs. ExPASy TrEMBL
Match: A0A2P5C0K2 (RmlC-like cupins superfamily protein OS=Trema orientale OX=63057 GN=TorRG33x02_301930 PE=4 SV=1)

HSP 1 Score: 182.6 bits (462), Expect = 9.3e-43
Identity = 84/105 (80.00%), Postives = 96/105 (91.43%), Query Frame = 0


Query: 8   SNNPNSTNNLRIIVERNPSQAKLSQLNIHCWPKWGCSAGKYQLKFEAEETCYLVKGKVKA 67
           SN+ +S  +LRIIVE+NPS+A+LS+LNI CWPKWGCS GKYQLKF+AEETCYL+KGKVKA
Sbjct: 11  SNSSSSGTSLRIIVEKNPSEARLSELNIKCWPKWGCSPGKYQLKFDAEETCYLLKGKVKA 70

Query: 68  YAKGSADSEYTEFGAGDLVTIPKGLSCTWDVSVAVDKFYKFESQS 113
           Y KGS+ SE+ EFGAGDLVTIPKGLSCTWDVSVAVDK+YKFES S
Sbjct: 71  YPKGSSSSEFVEFGAGDLVTIPKGLSCTWDVSVAVDKYYKFESSS 115

[Close]

BLAST of Sed0028023 vs. ExPASy TrEMBL
Match: A0A2P5AKT6 (RmlC-like cupins superfamily protein OS=Parasponia andersonii OX=3476 GN=PanWU01x14_322550 PE=4 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 4.6e-42
Identity = 84/111 (75.68%), Postives = 98/111 (88.29%), Query Frame = 0


Query: 2   ANEEGNSNNPNSTNNLRIIVERNPSQAKLSQLNIHCWPKWGCSAGKYQLKFEAEETCYLV 61
           ++   +S + +S  +LRIIVE+NPS+AKLS+LNI CWPKWGCS GKYQLKF+AEETCYL+
Sbjct: 6   SSSSSSSYSSSSGTSLRIIVEKNPSEAKLSELNIKCWPKWGCSPGKYQLKFDAEETCYLL 65

Query: 62  KGKVKAYAKGSADSEYTEFGAGDLVTIPKGLSCTWDVSVAVDKFYKFESQS 113
           KGKVKAY KGS+ SE+ EFGAGDLVTIPKGLSCTWDVSVAVDK+YKFES S
Sbjct: 66  KGKVKAYPKGSSSSEFVEFGAGDLVTIPKGLSCTWDVSVAVDKYYKFESSS 116

[Close]

BLAST of Sed0028023 vs. TAIR 10
Match: AT3G04300.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 138.3 bits (347), Expect = 3.9e-33
Identity = 62/93 (66.67%), Postives = 71/93 (76.34%), Query Frame = 0


Query: 17  LRIIVERNPSQAKLSQLNIHCWPKWGCSAGKYQLKFEAEETCYLVKGKVKAYAKGSADSE 76
           + I++E NPS  +LS L +  WPKW C  GKY L FE  ETCYLVKGKVK Y KGS  SE
Sbjct: 1   MNIVIENNPSSRRLSDLGVMSWPKWSCQPGKYALVFEERETCYLVKGKVKVYPKGS--SE 60

Query: 77  YTEFGAGDLVTIPKGLSCTWDVSVAVDKFYKFE 110
           + EFGAGDLVTIPKGLSCTWDVS+ +DK YKF+
Sbjct: 61  FVEFGAGDLVTIPKGLSCTWDVSLFIDKHYKFD 91

[Close]

BLAST of Sed0028023 vs. TAIR 10
Match: AT4G28703.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 135.2 bits (339), Expect = 3.3e-32
Identity = 65/102 (63.73%), Postives = 77/102 (75.49%), Query Frame = 0


Query: 16  NLRIIVERNPSQAKLSQLNIHCWPKWGCSAGKYQLKFEAEETCYLVKGKVKAYAK----G 75
           N RIIVE+NPSQA+L +L    WPKWGCS GKY LK+EAEE CY+++GKVK Y K     
Sbjct: 5   NPRIIVEQNPSQARLDELKFKSWPKWGCSPGKYHLKYEAEEICYILRGKVKVYPKPPPSS 64

Query: 76  SADSEY---TEFGAGDLVTIPKGLSCTWDVSVAVDKFYKFES 111
           S+D+E     EFGAGD+VT PKGLSCTWDVS++VDK Y F S
Sbjct: 65  SSDAEVEWCVEFGAGDIVTFPKGLSCTWDVSLSVDKHYIFLS 106

[Close]

BLAST of Sed0028023 vs. TAIR 10
Match: AT4G10300.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 119.0 bits (297), Expect = 2.4e-27
Identity = 59/117 (50.43%), Postives = 75/117 (64.10%), Query Frame = 0


Query: 2   ANEEGNSNNPN---------STNNLRIIVERNPSQAKLSQLNIHCWPKWGCSAGKYQLKF 61
           +N+  NS  P+         ST  L I +E+NP ++KL+QL +  WPKWGC   K+   +
Sbjct: 20  SNKPYNSRRPSSMAAAIRAESTEKLGITIEKNPPESKLTQLGVRSWPKWGCPPSKFPWTY 79

Query: 62  EAEETCYLVKGKVKAYAKGSADSEYTEFGAGDLVTIPKGLSCTWDVSVAVDKFYKFE 110
            A+ETCYL++GKVK Y  GS   E  E  AGD V  PKG+SCTWDVSVAVDK Y+FE
Sbjct: 80  SAKETCYLLQGKVKVYPNGS--DEGVEIEAGDFVVFPKGMSCTWDVSVAVDKHYQFE 134

The following BLAST results are available for this feature:

BLAST of Sed0028023 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7025258.1	1.0e-51	93.75	hypothetical protein SDJN02_11753 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6592849.1	1.3e-51	92.86	hypothetical protein SDJN03_12325, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022960185.1	1.7e-51	93.75	uncharacterized protein LOC111460998 [Cucurbita moschata] >XP_022960186.1 unchar...	[more]
XP_023005099.1	3.8e-51	92.86	uncharacterized protein LOC111498188 [Cucurbita maxima] >XP_023005100.1 uncharac...	[more]
XP_038906875.1	2.2e-46	83.62	uncharacterized protein LOC120092760 [Benincasa hispida]	[more]

BLAST of Sed0028023 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Sed0028023 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H6X6	8.4e-52	93.75	uncharacterized protein LOC111460998 OS=Cucurbita moschata OX=3662 GN=LOC1114609...	[more]
A0A6J1KU03	1.9e-51	92.86	uncharacterized protein LOC111498188 OS=Cucurbita maxima OX=3661 GN=LOC111498188...	[more]
A0A1S3CBJ5	8.9e-46	79.20	uncharacterized protein LOC103498932 OS=Cucumis melo OX=3656 GN=LOC103498932 PE=...	[more]
A0A2P5C0K2	9.3e-43	80.00	RmlC-like cupins superfamily protein OS=Trema orientale OX=63057 GN=TorRG33x02_3...	[more]
A0A2P5AKT6	4.6e-42	75.68	RmlC-like cupins superfamily protein OS=Parasponia andersonii OX=3476 GN=PanWU01...	[more]

BLAST of Sed0028023 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G04300.1	3.9e-33	66.67	RmlC-like cupins superfamily protein	[more]
AT4G28703.1	3.3e-32	63.73	RmlC-like cupins superfamily protein	[more]
AT4G10300.1	2.4e-27	50.43	RmlC-like cupins superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008579	(S)-ureidoglycine aminohydrolase, cupin-3 domain	PFAM	PF05899	Cupin_3	coord: 31..106 e-value: 1.2E-25 score: 89.0
IPR014710	RmlC-like jelly roll fold	GENE3D	2.60.120.10	Jelly Rolls	coord: 6..108 e-value: 8.1E-34 score: 117.3
None	No IPR available	PANTHER	PTHR33271:SF3	OS02G0620400 PROTEIN	coord: 1..111
None	No IPR available	PANTHER	PTHR33271	OS04G0445200 PROTEIN	coord: 1..111
None	No IPR available	CDD	cd02227	cupin_TM1112-like	coord: 38..108 e-value: 2.19171E-25 score: 88.7915
IPR011051	RmlC-like cupin domain superfamily	SUPERFAMILY	51182	RmlC-like cupins	coord: 29..106

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sed0028023.1	Sed0028023.1	mRNA