Sed0027898 (gene) Chayote v1

BLAST of Sed0027898 vs. NCBI nr
Match: XP_038876500.1 (lectin-like [Benincasa hispida])

HSP 1 Score: 90.5 bits (223), Expect = 1.5e-14
Identity = 57/153 (37.25%), Postives = 81/153 (52.94%), Query Frame = 0

Query: 11  VFNARDLEIAWSEDPTRWKWVKVDVPRPPIQPQEAAELLGVNWLEVRKIVTVEKLVFSNA 70
           +  ARDL IAWSEDP  WKW   ++    +   E AEL+ V WL++R  +  EKL  S  
Sbjct: 158 IIPARDLAIAWSEDPRYWKWTFKEINGKKV---EVAELIYVWWLDIRGSIKAEKL--SPG 217

Query: 71  EDYYAAFEVMMKEDASGWEAPVTLTWKKLDSEQTPHIIYQDMHMLPTNKWVPLIVGGFKH 130
             Y   FE+++KE    W+ PV L  K   S+    +  + +     + W P+ VG  K 
Sbjct: 218 ITYEILFELLLKESRYDWKNPVNLKLKW--SDGLTIVTNESLENKQRDVWFPIKVGEVK- 277

Query: 131 ADIEQTGIEFSMSQHD-NFVKKGLVVRSLVITP 163
            D     + F++  HD N+VK+GLVVR+ VI P
Sbjct: 278 VDDGVGELTFTLYDHDGNYVKEGLVVRAAVIQP 302

BLAST of Sed0027898 vs. NCBI nr
Match: C0HJV2.1 (RecName: Full=Lectin; AltName: Full=Agglutinin; Short=LAA [Luffa acutangula])

HSP 1 Score: 90.5 bits (223), Expect = 1.5e-14
Identity = 64/155 (41.29%), Postives = 79/155 (50.97%), Query Frame = 0

Query: 14  ARDLEIAWSEDPTRWKWVKVDVPRPPIQPQ----EAAELLGVNWLEVRKIVTVEKLVFSN 73
           ARDL I WS+D   W+W        P Q      E AEL+ V WL +  +  +E  V S 
Sbjct: 66  ARDLLITWSQDKRYWRW-------NPFQEHGNTLEVAELIDVCWLNI--VGNIETSVLSP 125

Query: 74  AEDYYAAFEVMMKEDASGWEAPVTLTWKKLD-SEQTPHIIYQDMHMLPTNKWVPLIVGGF 133
              Y AAFEVM+   ASGW  PV +  K  D SEQ   +  QD    P   W  + VG F
Sbjct: 126 GISYEAAFEVMLTNSASGWRIPVDVKLKMPDGSEQESQVNLQDK---PRGVWFFISVGHF 185

Query: 134 KHADIEQTG-IEFSMSQHDNFVKKGLVVRSLVITP 163
           K +  E  G IEFS+ QH    K+GL+V+ LVI P
Sbjct: 186 KISVGETIGNIEFSIVQHQE-AKRGLLVKGLVIQP 207

BLAST of Sed0027898 vs. NCBI nr
Match: BAK79141.1 (phloem lectin-like protein [Benincasa hispida])

HSP 1 Score: 90.1 bits (222), Expect = 1.9e-14
Identity = 57/153 (37.25%), Postives = 81/153 (52.94%), Query Frame = 0

Query: 11  VFNARDLEIAWSEDPTRWKWVKVDVPRPPIQPQEAAELLGVNWLEVRKIVTVEKLVFSNA 70
           +  ARDL IAWSEDP  WKW   ++    +   E AEL+ V WL++R  +  EKL  S  
Sbjct: 158 IIPARDLAIAWSEDPRYWKWTFKEINGKKV---EVAELIYVWWLDIRGSIKAEKL--SPG 217

Query: 71  EDYYAAFEVMMKEDASGWEAPVTLTWKKLDSEQTPHIIYQDMHMLPTNKWVPLIVGGFKH 130
             Y   FE+++KE    W+ PV L  K   S+    +  + +     + W P+ VG  K 
Sbjct: 218 ITYEILFELLLKESRYDWKNPVNLKLKW--SDGLTIVTNESLENKQRDVWFPIKVGEVK- 277

Query: 131 ADIEQTGIEFSMSQHD-NFVKKGLVVRSLVITP 163
            D     + F++  HD N+VK+GLVVR+ VI P
Sbjct: 278 VDDGIGELTFTLYDHDGNYVKEGLVVRAAVIQP 302

BLAST of Sed0027898 vs. NCBI nr
Match: XP_011626594.1 (uncharacterized protein LOC105421323 [Amborella trichopoda])

HSP 1 Score: 88.2 bits (217), Expect = 7.2e-14
Identity = 57/151 (37.75%), Postives = 78/151 (51.66%), Query Frame = 0

Query: 14  ARDLEIAWSEDPTRWKWVKVDVPRPPIQPQEAAELLGVNWLEVRKIVTVEKLVFSNAEDY 73
           ARDL+I W ED   W+W++  +        E AELL V W EVR    ++   FS   DY
Sbjct: 97  ARDLKIVWGEDGRYWRWIR--LAETSHSKIEIAELLNVCWFEVRG--KIDASWFSPGIDY 156

Query: 74  YAAFEVMMKEDASGWEA-PVTLTWKKLDSEQTPHIIYQDMHMLPTNKWVPLIVGGFKHAD 133
             AF V + E+A GWEA  VTL+    +  +  H   Q++      KW+ + VG F H  
Sbjct: 157 IVAFIVKLGEEAFGWEAQDVTLSLTCANKMEKTHT--QNLEEKERGKWMEIHVGAFHHGV 216

Query: 134 IEQTGIEFSMSQ-HDNFVKKGLVVRSLVITP 163
            E+  IEFSM    D+  K GL ++ +VI P
Sbjct: 217 EEKGDIEFSMMHTQDSRWKSGLFIKGVVIRP 241

BLAST of Sed0027898 vs. NCBI nr
Match: XP_038877540.1 (lectin-like [Benincasa hispida])

HSP 1 Score: 87.4 bits (215), Expect = 1.2e-13
Identity = 50/148 (33.78%), Postives = 77/148 (52.03%), Query Frame = 0

Query: 14  ARDLEIAWSEDPTRWKWVKVDVPRPPIQPQEAAELLGVNWLEVRKIVTVEKLVFSNAEDY 73
           ARDL IAW +DP  WKW  +++    +   E AEL+ V WL +   + V+KL  S    Y
Sbjct: 14  ARDLTIAWIDDPQYWKWTSIEIDGKKV---EVAELIRVYWLNIGGSINVQKL--SPGITY 73

Query: 74  YAAFEVMMKEDASGWEAPVTLTWKKLDSEQTPHIIYQDMHMLPTNKWVPLIVGGFKHADI 133
              F+V++KE A  W+ PV L  K+ D      + ++ +     + W  + VG FK  D+
Sbjct: 74  EIVFDVLLKESAYDWKNPVNLELKQPDG--LTIVTHESLENQTRDTWFQIKVGEFKVDDV 133

Query: 134 EQTGIEFSMSQHDNFVKKGLVVRSLVIT 162
               + F++ +H  + K GLVVR   I+
Sbjct: 134 GGK-LAFTLYEHGQYWKSGLVVRGAEIS 153

BLAST of Sed0027898 vs. ExPASy Swiss-Prot
Match: C0HJV2 (Lectin OS=Luffa acutangula OX=56866 PE=1 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 1.9e-17
Identity = 64/155 (41.29%), Postives = 79/155 (50.97%), Query Frame = 0

Query: 14  ARDLEIAWSEDPTRWKWVKVDVPRPPIQPQ----EAAELLGVNWLEVRKIVTVEKLVFSN 73
           ARDL I WS+D   W+W        P Q      E AEL+ V WL +  +  +E  V S 
Sbjct: 66  ARDLLITWSQDKRYWRW-------NPFQEHGNTLEVAELIDVCWLNI--VGNIETSVLSP 125

Query: 74  AEDYYAAFEVMMKEDASGWEAPVTLTWKKLD-SEQTPHIIYQDMHMLPTNKWVPLIVGGF 133
              Y AAFEVM+   ASGW  PV +  K  D SEQ   +  QD    P   W  + VG F
Sbjct: 126 GISYEAAFEVMLTNSASGWRIPVDVKLKMPDGSEQESQVNLQDK---PRGVWFFISVGHF 185

Query: 134 KHADIEQTG-IEFSMSQHDNFVKKGLVVRSLVITP 163
           K +  E  G IEFS+ QH    K+GL+V+ LVI P
Sbjct: 186 KISVGETIGNIEFSIVQHQE-AKRGLLVKGLVIQP 207

BLAST of Sed0027898 vs. ExPASy Swiss-Prot
Match: Q9FHE8 (Protein PHLOEM PROTEIN 2-LIKE A6 OS=Arabidopsis thaliana OX=3702 GN=PP2A6 PE=2 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 5.4e-12
Identity = 52/164 (31.71%), Postives = 79/164 (48.17%), Query Frame = 0

Query: 3   ENFVITTTVFNARDLEIAWSEDPTRWKWVKVDVPRPPIQPQEAAELLGVNWLEVRKIVTV 62
           E   +  ++  ARDL+I  SE P +W W  ++   P     E A L  V WL++   +T 
Sbjct: 230 EGSSVVPSMVPARDLDITHSEKPQKWTWSTIN-EAPNSAEIEIATLNKVYWLKIVGTITT 289

Query: 63  EKLVFSNAEDYYAAFEVMMKEDASGWEAPVTLTWKKL--DSEQTPHIIYQDMHMLPTNKW 122
           E L  +    Y A F V ++ +ASGWE PV L  K +  D +       ++++      W
Sbjct: 290 ENL--TPGAKYEAVFVVKLENNASGWEQPVNLKLKVVQHDGDDDRVDRTENLNDYIGQNW 349

Query: 123 VPLIVGGF-KHADIEQTGIEFSMSQH-DNFVKKGLVVRSLVITP 163
           V ++ G F          I F+M Q+ D + KKGLVV+ + I P
Sbjct: 350 VDILAGVFVVPPKTTPATIIFTMYQYEDKYKKKGLVVKGVAIRP 390

BLAST of Sed0027898 vs. ExPASy Swiss-Prot
Match: Q9C8U9 (Uncharacterized protein PHLOEM PROTEIN 2-LIKE A4 OS=Arabidopsis thaliana OX=3702 GN=PP2A4 PE=4 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 5.6e-09
Identity = 42/150 (28.00%), Postives = 65/150 (43.33%), Query Frame = 0

Query: 14  ARDLEIAWSEDPTRWKWVKVDVPRPPIQPQEAAELLGVNWLEVRKIVTVEKLVFSNAEDY 73
           ARDL IAWS+    W W+ +       +  +AA L  V WL+V       +L       Y
Sbjct: 16  ARDLSIAWSDKDEYWSWLPLRYDISSEKLVDAAVLEAVCWLDVNGKFDTRELTLETT--Y 75

Query: 74  YAAFEVMMKEDASGWEAPVTLTWKKLDSEQTPHIIYQDMHMLPTNKWVPLIVGGFKHADI 133
              + V +++ ASGW  PV L     D ++ P      +      +W+ +  G F  +  
Sbjct: 76  EVVYVVKLEDTASGWNIPVNLKLTLPDGKKRPQERSMCLKEHIGKRWIDISAGEFVTSPD 135

Query: 134 EQTGIEFSMSQHDNFV-KKGLVVRSLVITP 163
               I FSM +  +   K+GL V+ + I P
Sbjct: 136 NAGEISFSMYETKSCCWKRGLFVKCVEIRP 163

BLAST of Sed0027898 vs. ExPASy Swiss-Prot
Match: Q9C5Q9 (Protein PHLOEM PROTEIN 2-LIKE A5 OS=Arabidopsis thaliana OX=3702 GN=PP2A5 PE=2 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 2.1e-08
Identity = 43/153 (28.10%), Postives = 64/153 (41.83%), Query Frame = 0

Query: 11  VFNARDLEIAWSEDPTRWKWVKVDVPRPPIQPQEAAELLGVNWLEVRKIVTVEKLVFSNA 70
           + +ARDL IAWSED   W W+ +          E A L   +WL+V        L     
Sbjct: 260 MIDARDLSIAWSEDSNHWTWLPLPNQNSNESVMEIAFLKSASWLDVAGKFDTRYL---TP 319

Query: 71  EDYYAAFEVMMKEDASGWEAPVTLTWKKLDSEQTPHIIYQDMHMLPTNKWVPLIVGGFKH 130
              Y    V+  E    WE  V L     ++ + P     DM    +++W+ + VG F  
Sbjct: 320 RTRYEVVFVVKLEYTFEWETLVKLKLDLPNTWEKPQEQSVDMFDYISDQWLDIPVGEFTT 379

Query: 131 ADIEQTGIEFSMSQHD-NFVKKGLVVRSLVITP 163
           +      I F+M +H+    K GL V+ + I P
Sbjct: 380 SKKNVGEISFAMYEHECQLWKSGLFVKGVTIRP 409

BLAST of Sed0027898 vs. ExPASy TrEMBL
Match: G1UJV7 (Phloem lectin-like protein OS=Benincasa hispida OX=102211 GN=bplp PE=2 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 9.2e-15
Identity = 57/153 (37.25%), Postives = 81/153 (52.94%), Query Frame = 0

Query: 11  VFNARDLEIAWSEDPTRWKWVKVDVPRPPIQPQEAAELLGVNWLEVRKIVTVEKLVFSNA 70
           +  ARDL IAWSEDP  WKW   ++    +   E AEL+ V WL++R  +  EKL  S  
Sbjct: 158 IIPARDLAIAWSEDPRYWKWTFKEINGKKV---EVAELIYVWWLDIRGSIKAEKL--SPG 217

Query: 71  EDYYAAFEVMMKEDASGWEAPVTLTWKKLDSEQTPHIIYQDMHMLPTNKWVPLIVGGFKH 130
             Y   FE+++KE    W+ PV L  K   S+    +  + +     + W P+ VG  K 
Sbjct: 218 ITYEILFELLLKESRYDWKNPVNLKLKW--SDGLTIVTNESLENKQRDVWFPIKVGEVK- 277

Query: 131 ADIEQTGIEFSMSQHD-NFVKKGLVVRSLVITP 163
            D     + F++  HD N+VK+GLVVR+ VI P
Sbjct: 278 VDDGIGELTFTLYDHDGNYVKEGLVVRAAVIQP 302

BLAST of Sed0027898 vs. ExPASy TrEMBL
Match: A0A6J1CN87 (lectin-like OS=Momordica charantia OX=3673 GN=LOC111012568 PE=4 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 6.0e-14
Identity = 54/148 (36.49%), Postives = 79/148 (53.38%), Query Frame = 0

Query: 15  RDLEIAWSEDPTRWKWVKVDVPRPPIQPQEAAELLGVNWLEVRKIVTVEKLVFSNAEDYY 74
           R L I WSE+   WKW  ++    PI   EA EL+ V WLE+   +   +L  S    Y 
Sbjct: 93  RALSITWSEESKYWKWKAMEESSNPI---EAIELVNVCWLEIHGKIKASEL--SPGAWYE 152

Query: 75  AAFEVMMKEDASGWEAPVTLTWKKLDSEQTPHIIYQDMHMLPTNKWVPLIVGGFKHADIE 134
           AAF VM+K+ A GW+ PV +  K+ D  +      +DM   P  +WV + +G F   D +
Sbjct: 153 AAFVVMIKDPAYGWDVPVNIRLKRPDGSKQER--KEDMEEKPRGRWVEIPIGDFTVQDHD 212

Query: 135 QTG-IEFSMSQHDNFV-KKGLVVRSLVI 161
             G IEFSM +++    KKG+ ++ +VI
Sbjct: 213 NGGEIEFSMYEYEGGQWKKGMFLKGVVI 233

BLAST of Sed0027898 vs. ExPASy TrEMBL
Match: A0A5H2XQI9 (Phloem protein 2-A1 OS=Prunus dulcis OX=3755 GN=Prudu_1438S001200 PE=4 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 1.1e-12
Identity = 54/152 (35.53%), Postives = 75/152 (49.34%), Query Frame = 0

Query: 14  ARDLEIAWSEDPTRWKWVKVDVPRPPIQPQEAAELLGVNWLEVR-KIVTVEKLVFSNAED 73
           A+ L+I WS D   W+W K           EAAELL V WLEVR K+ T +    S    
Sbjct: 64  AKALDITWSGDTRYWRWAK----ETSGDDFEAAELLNVCWLEVRGKLATTD---LSPGTL 123

Query: 74  YYAAFEVMMKEDASGWEAPVTLTWKKLDSEQTPHIIYQDMHMLPTNK--WVPLIVGGFKH 133
           Y   F V MK +A GW+APV   +   D   T +  ++ M +L  +K  W  +  G FK 
Sbjct: 124 YEVVFVVKMKTEADGWDAPVNFKFTPPDGAVTRNTTFKLMEILKDSKDEWKDIPFGEFK- 183

Query: 134 ADIEQTGIEFSMSQHDNFVKKGLVVRSLVITP 163
           A      IEFS+ ++    K GLV++ + + P
Sbjct: 184 APENPRNIEFSLYEYSRIWKSGLVIKGVAVRP 207

BLAST of Sed0027898 vs. ExPASy TrEMBL
Match: A0A1S3CQJ8 (protein PHLOEM PROTEIN 2-LIKE A1-like OS=Cucumis melo OX=3656 GN=LOC103503156 PE=4 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 1.5e-12
Identity = 53/149 (35.57%), Postives = 80/149 (53.69%), Query Frame = 0

Query: 15  RDLEIAWSEDPTRWKWVKVDVPRPPIQPQEAAELLGVNWLEVR-KIVTVEKLVFSNAEDY 74
           R L I W+E+   W+W  +      I   E  EL+ V WLE+  K+ T E    S    Y
Sbjct: 115 RALSITWAEENKYWRWRPLQDSSNTI---EVVELMNVCWLEIHGKMKTCE---LSPGICY 174

Query: 75  YAAFEVMMKEDASGWEAPVTLTWKKLDSEQTPHIIYQDMHMLPTNKWVPLIVGGFKHADI 134
            AAFEVM+K+ A GW+ PV +  KK D  +  H   +++   P  +W  + +G F   D 
Sbjct: 175 EAAFEVMIKDPAYGWDIPVNIRVKKPDGSKQEH--QENLEQRPRGRWFEIPIGNFIVRDH 234

Query: 135 EQTG-IEFSMSQHDNFV-KKGLVVRSLVI 161
           E+ G IEF M +++  + KKG+V++ +VI
Sbjct: 235 ERGGEIEFCMFEYEGGMWKKGMVLKGVVI 255

BLAST of Sed0027898 vs. ExPASy TrEMBL
Match: A0A7J6GE37 (Uncharacterized protein OS=Cannabis sativa OX=3483 GN=F8388_003167 PE=4 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 2.5e-12
Identity = 57/151 (37.75%), Postives = 71/151 (47.02%), Query Frame = 0

Query: 14  ARDLEIAWSEDPTRWKWVK-VDVPRPPIQPQEAAELLGVNWLEVRKIVTVEKLVFSNAED 73
           AR L I W ED   W W +  D     I   E A L  V WLEV     + KL  S    
Sbjct: 106 ARKLSITWGEDNRYWTWQQHKDTSEELI---EVARLKQVCWLEVNGKFEMRKL--SPVTL 165

Query: 74  YYAAFEVMMKEDASGWEAPVTLTWKKLDSEQTPHIIYQDMHMLPTNKWVPLIVGGFK--- 133
           Y  AF VMM + A+GW  PV ++    D     H   QDM  LPTN+W  +  G FK   
Sbjct: 166 YEVAFVVMMDDSANGWSFPVNISLSLPDGTNQQH--NQDMTKLPTNQWTHITAGKFKTSH 225

Query: 134 HADIEQTGIEFSMSQHDNFVKKGLVVRSLVI 161
           H D     I FSM  +D+  K GL ++ ++I
Sbjct: 226 HNDQFGEDIHFSMHNYDSPWKAGLSIKGVLI 249

BLAST of Sed0027898 vs. TAIR 10
Match: AT5G45080.1 (phloem protein 2-A6 )

HSP 1 Score: 72.4 bits (176), Expect = 3.8e-13
Identity = 52/164 (31.71%), Postives = 79/164 (48.17%), Query Frame = 0

Query: 3   ENFVITTTVFNARDLEIAWSEDPTRWKWVKVDVPRPPIQPQEAAELLGVNWLEVRKIVTV 62
           E   +  ++  ARDL+I  SE P +W W  ++   P     E A L  V WL++   +T 
Sbjct: 230 EGSSVVPSMVPARDLDITHSEKPQKWTWSTIN-EAPNSAEIEIATLNKVYWLKIVGTITT 289

Query: 63  EKLVFSNAEDYYAAFEVMMKEDASGWEAPVTLTWKKL--DSEQTPHIIYQDMHMLPTNKW 122
           E L  +    Y A F V ++ +ASGWE PV L  K +  D +       ++++      W
Sbjct: 290 ENL--TPGAKYEAVFVVKLENNASGWEQPVNLKLKVVQHDGDDDRVDRTENLNDYIGQNW 349

Query: 123 VPLIVGGF-KHADIEQTGIEFSMSQH-DNFVKKGLVVRSLVITP 163
           V ++ G F          I F+M Q+ D + KKGLVV+ + I P
Sbjct: 350 VDILAGVFVVPPKTTPATIIFTMYQYEDKYKKKGLVVKGVAIRP 390

BLAST of Sed0027898 vs. TAIR 10
Match: AT1G33920.1 (phloem protein 2-A4 )

HSP 1 Score: 62.4 bits (150), Expect = 4.0e-10
Identity = 42/150 (28.00%), Postives = 65/150 (43.33%), Query Frame = 0

Query: 14  ARDLEIAWSEDPTRWKWVKVDVPRPPIQPQEAAELLGVNWLEVRKIVTVEKLVFSNAEDY 73
           ARDL IAWS+    W W+ +       +  +AA L  V WL+V       +L       Y
Sbjct: 16  ARDLSIAWSDKDEYWSWLPLRYDISSEKLVDAAVLEAVCWLDVNGKFDTRELTLETT--Y 75

Query: 74  YAAFEVMMKEDASGWEAPVTLTWKKLDSEQTPHIIYQDMHMLPTNKWVPLIVGGFKHADI 133
              + V +++ ASGW  PV L     D ++ P      +      +W+ +  G F  +  
Sbjct: 76  EVVYVVKLEDTASGWNIPVNLKLTLPDGKKRPQERSMCLKEHIGKRWIDISAGEFVTSPD 135

Query: 134 EQTGIEFSMSQHDNFV-KKGLVVRSLVITP 163
               I FSM +  +   K+GL V+ + I P
Sbjct: 136 NAGEISFSMYETKSCCWKRGLFVKCVEIRP 163

BLAST of Sed0027898 vs. TAIR 10
Match: AT1G65390.1 (phloem protein 2 A5 )

HSP 1 Score: 60.5 bits (145), Expect = 1.5e-09
Identity = 43/153 (28.10%), Postives = 64/153 (41.83%), Query Frame = 0

Query: 11  VFNARDLEIAWSEDPTRWKWVKVDVPRPPIQPQEAAELLGVNWLEVRKIVTVEKLVFSNA 70
           + +ARDL IAWSED   W W+ +          E A L   +WL+V        L     
Sbjct: 260 MIDARDLSIAWSEDSNHWTWLPLPNQNSNESVMEIAFLKSASWLDVAGKFDTRYL---TP 319

Query: 71  EDYYAAFEVMMKEDASGWEAPVTLTWKKLDSEQTPHIIYQDMHMLPTNKWVPLIVGGFKH 130
              Y    V+  E    WE  V L     ++ + P     DM    +++W+ + VG F  
Sbjct: 320 RTRYEVVFVVKLEYTFEWETLVKLKLDLPNTWEKPQEQSVDMFDYISDQWLDIPVGEFTT 379

Query: 131 ADIEQTGIEFSMSQHD-NFVKKGLVVRSLVITP 163
           +      I F+M +H+    K GL V+ + I P
Sbjct: 380 SKKNVGEISFAMYEHECQLWKSGLFVKGVTIRP 409