Sed0027781 (gene) Chayote v1

BLAST of Sed0027781 vs. NCBI nr
Match: KAG6594711.1 (U-box domain-containing protein 33, partial [Cucurbita argyrosperma subsp. sororia] >KAG7026677.1 U-box domain-containing protein 33, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 477.6 bits (1228), Expect = 7.2e-131
Identity = 250/289 (86.51%), Postives = 261/289 (90.31%), Query Frame = 0

Query: 1   MSTLPSPAEAGHFHPDEGLGSEFSNYRY-NSTSEIEEEGSAETFEIKRQMLSPA------ 60
           MS LPSPAEAG+FH D+G GSEFS YRY +STSEIEEE SA+TFEIKRQ +S A      
Sbjct: 1   MSALPSPAEAGNFHGDDGFGSEFSTYRYSSSTSEIEEEDSAQTFEIKRQTVSAAAAAGAG 60

Query: 61  MEISREDYSLGCSFSSDALKWEDCVYVGVGRNDSSFDALQWTLKNTITNSTTVVYLLHVF 120
           +E  REDYS GCSFSSDALKWEDCVYVGVG++DSS +ALQW LKN IT STTVVYLLHVF
Sbjct: 61  LESIREDYSRGCSFSSDALKWEDCVYVGVGKSDSSNEALQWALKNAITTSTTVVYLLHVF 120

Query: 121 PETRYIPSPLGKIPINQVSKQQVAIHAAQEESKRKDFLQNFIDSCSAVKVKADTVLIESD 180
           PETRYIPSPLGKIPINQVSKQQVAIH AQEESKRKDFLQNFIDSCS  KVKADTVLIESD
Sbjct: 121 PETRYIPSPLGKIPINQVSKQQVAIHVAQEESKRKDFLQNFIDSCSLAKVKADTVLIESD 180

Query: 181 IVARAILDVIPILNIRKLVLGVNKSRKLRSRGGSGIANEILQKAPAFCEVKVVCEGKETS 240
           +VARAILDVIPILNIRKLVLG NKSRKLRSRGGSGIANEILQKAP FCEVKVVCEGKETS
Sbjct: 181 MVARAILDVIPILNIRKLVLGANKSRKLRSRGGSGIANEILQKAPEFCEVKVVCEGKETS 240

Query: 241 QLGGSPSPLSSPRYQDDSFKDNDPNSTITVAEEQRNSSVSCMCFKTKFV 283
           QLGG PSP+SSPRYQDDS KDNDPNSTITVAEEQRNSSVSCMCFKTKFV
Sbjct: 241 QLGGLPSPISSPRYQDDSSKDNDPNSTITVAEEQRNSSVSCMCFKTKFV 289

BLAST of Sed0027781 vs. NCBI nr
Match: XP_023518700.1 (U-box domain-containing protein 35-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 475.3 bits (1222), Expect = 3.6e-130
Identity = 248/289 (85.81%), Postives = 261/289 (90.31%), Query Frame = 0

Query: 1   MSTLPSPAEAGHFHPDEGLGSEFSNYRY-NSTSEIEEEGSAETFEIKRQMLSPA------ 60
           MS LPSPAEAG+ H D+G GSEF  YRY +STSEIEEE SA+TFEIKRQ +S A      
Sbjct: 1   MSALPSPAEAGNIHGDDGFGSEFRTYRYSSSTSEIEEEDSAQTFEIKRQTVSAAAAAGAG 60

Query: 61  MEISREDYSLGCSFSSDALKWEDCVYVGVGRNDSSFDALQWTLKNTITNSTTVVYLLHVF 120
           +E  REDYS GCSFSSDALKWEDCVYVGVG++DSS +ALQWTLKN IT+STTVVYLLHVF
Sbjct: 61  LESIREDYSRGCSFSSDALKWEDCVYVGVGKSDSSNEALQWTLKNAITSSTTVVYLLHVF 120

Query: 121 PETRYIPSPLGKIPINQVSKQQVAIHAAQEESKRKDFLQNFIDSCSAVKVKADTVLIESD 180
           PETRYIPSPLGKIP+NQVSKQQVAIH AQEESKRKDFLQNFIDSCS  KVKADTVLIESD
Sbjct: 121 PETRYIPSPLGKIPVNQVSKQQVAIHVAQEESKRKDFLQNFIDSCSLAKVKADTVLIESD 180

Query: 181 IVARAILDVIPILNIRKLVLGVNKSRKLRSRGGSGIANEILQKAPAFCEVKVVCEGKETS 240
           +VARAILDVIPILNIRKLVLG NKSRKLRSRGGSGIANEILQKAP FCEVKVVCEGKETS
Sbjct: 181 MVARAILDVIPILNIRKLVLGANKSRKLRSRGGSGIANEILQKAPEFCEVKVVCEGKETS 240

Query: 241 QLGGSPSPLSSPRYQDDSFKDNDPNSTITVAEEQRNSSVSCMCFKTKFV 283
           QLGG PSP+SSPRYQDDS KDNDPNSTITVAEEQRNSSVSCMCFKTKFV
Sbjct: 241 QLGGLPSPISSPRYQDDSSKDNDPNSTITVAEEQRNSSVSCMCFKTKFV 289

BLAST of Sed0027781 vs. NCBI nr
Match: XP_022926787.1 (U-box domain-containing protein 35-like isoform X4 [Cucurbita moschata])

HSP 1 Score: 474.2 bits (1219), Expect = 8.0e-130
Identity = 248/289 (85.81%), Postives = 260/289 (89.97%), Query Frame = 0

Query: 1   MSTLPSPAEAGHFHPDEGLGSEFSNYRY-NSTSEIEEEGSAETFEIKRQMLSPA------ 60
           MS LPSPAEAG+FH D+G GSEFS YRY +STSEIEEE SA+TFEIKRQ +S A      
Sbjct: 1   MSALPSPAEAGNFHGDDGFGSEFSTYRYSSSTSEIEEEDSAQTFEIKRQTVSAAAAAGAG 60

Query: 61  MEISREDYSLGCSFSSDALKWEDCVYVGVGRNDSSFDALQWTLKNTITNSTTVVYLLHVF 120
           +E  REDYS GCSFSSDALKWEDCVYVGVG++DSS +ALQW LKN IT STTVVYLLHVF
Sbjct: 61  LESIREDYSRGCSFSSDALKWEDCVYVGVGKSDSSNEALQWALKNAITTSTTVVYLLHVF 120

Query: 121 PETRYIPSPLGKIPINQVSKQQVAIHAAQEESKRKDFLQNFIDSCSAVKVKADTVLIESD 180
           PETRYIPSPLGKIP+NQVSKQQVAIH AQEESKRKDFLQNFIDSCS  KVKADTVLIESD
Sbjct: 121 PETRYIPSPLGKIPVNQVSKQQVAIHVAQEESKRKDFLQNFIDSCSLAKVKADTVLIESD 180

Query: 181 IVARAILDVIPILNIRKLVLGVNKSRKLRSRGGSGIANEILQKAPAFCEVKVVCEGKETS 240
           +VARAILDVIPILNIRKLVLG NKSRKLRSRGGSGIANEILQKAP FCEVKVVCE KETS
Sbjct: 181 MVARAILDVIPILNIRKLVLGANKSRKLRSRGGSGIANEILQKAPEFCEVKVVCERKETS 240

Query: 241 QLGGSPSPLSSPRYQDDSFKDNDPNSTITVAEEQRNSSVSCMCFKTKFV 283
           QLGG PSP+SSPRYQDDS KDNDPNSTITVAEEQRNSSVSCMCFKTKFV
Sbjct: 241 QLGGLPSPISSPRYQDDSSKDNDPNSTITVAEEQRNSSVSCMCFKTKFV 289

BLAST of Sed0027781 vs. NCBI nr
Match: XP_023003371.1 (U-box domain-containing protein 35-like isoform X4 [Cucurbita maxima])

HSP 1 Score: 469.2 bits (1206), Expect = 2.6e-128
Identity = 246/290 (84.83%), Postives = 260/290 (89.66%), Query Frame = 0

Query: 1   MSTLPSPAEAGHFHPDEGLGSEFSNYRYN-STSEIEEEGSAETFEIKRQMLSPA------ 60
           MS LPSPA AG+F  ++G GSEF+ YRYN STSEIEEE SA+TFEIKR+ +S A      
Sbjct: 1   MSALPSPAGAGNFRGNDGFGSEFNTYRYNSSTSEIEEEDSAQTFEIKRKTVSAAAAAAGA 60

Query: 61  -MEISREDYSLGCSFSSDALKWEDCVYVGVGRNDSSFDALQWTLKNTITNSTTVVYLLHV 120
            +E  REDYS GCSFSSDALKWEDCVYVGVG++DSS +ALQW LKN IT+STTVVYLLHV
Sbjct: 61  GLESIREDYSRGCSFSSDALKWEDCVYVGVGKSDSSNEALQWALKNAITSSTTVVYLLHV 120

Query: 121 FPETRYIPSPLGKIPINQVSKQQVAIHAAQEESKRKDFLQNFIDSCSAVKVKADTVLIES 180
           FPETRYIPSPLGKIPINQVSKQQVAIH AQEESKRKDFLQNFIDSCS  KVKADTVLIES
Sbjct: 121 FPETRYIPSPLGKIPINQVSKQQVAIHVAQEESKRKDFLQNFIDSCSLAKVKADTVLIES 180

Query: 181 DIVARAILDVIPILNIRKLVLGVNKSRKLRSRGGSGIANEILQKAPAFCEVKVVCEGKET 240
           D+VARAILDVIPILNIRKLVLG NKSRKLRSRGGSGIANEILQKAP FCEVKVVCEGKET
Sbjct: 181 DMVARAILDVIPILNIRKLVLGANKSRKLRSRGGSGIANEILQKAPEFCEVKVVCEGKET 240

Query: 241 SQLGGSPSPLSSPRYQDDSFKDNDPNSTITVAEEQRNSSVSCMCFKTKFV 283
           SQLGG PSP+SSPRYQDDS KDNDPNSTITVAEEQRNSSVSCMCFKTKFV
Sbjct: 241 SQLGGLPSPISSPRYQDDSSKDNDPNSTITVAEEQRNSSVSCMCFKTKFV 290

BLAST of Sed0027781 vs. NCBI nr
Match: XP_022926785.1 (uncharacterized protein LOC111433795 isoform X2 [Cucurbita moschata])

HSP 1 Score: 460.7 bits (1184), Expect = 9.2e-126
Identity = 248/313 (79.23%), Postives = 260/313 (83.07%), Query Frame = 0

Query: 1   MSTLPSPAEAGHFHPDEGLGSEFSNYRY-NSTSEIEEEGSAETFEIKRQMLSPA------ 60
           MS LPSPAEAG+FH D+G GSEFS YRY +STSEIEEE SA+TFEIKRQ +S A      
Sbjct: 1   MSALPSPAEAGNFHGDDGFGSEFSTYRYSSSTSEIEEEDSAQTFEIKRQTVSAAAAAGAG 60

Query: 61  MEISREDYSLGCSFSSDALKWEDCVYVGVGRNDSSFDALQWTLKNTITNSTTVVYLLHVF 120
           +E  REDYS GCSFSSDALKWEDCVYVGVG++DSS +ALQW LKN IT STTVVYLLHVF
Sbjct: 61  LESIREDYSRGCSFSSDALKWEDCVYVGVGKSDSSNEALQWALKNAITTSTTVVYLLHVF 120

Query: 121 PETRYIPSPLGKIPINQVSKQQVAIHAAQEESKRKDFLQNFIDSCSAVK----------- 180
           PETRYIPSPLGKIP+NQVSKQQVAIH AQEESKRKDFLQNFIDSCS  K           
Sbjct: 121 PETRYIPSPLGKIPVNQVSKQQVAIHVAQEESKRKDFLQNFIDSCSLAKVHNRKACPLFA 180

Query: 181 -------------VKADTVLIESDIVARAILDVIPILNIRKLVLGVNKSRKLRSRGGSGI 240
                        VKADTVLIESD+VARAILDVIPILNIRKLVLG NKSRKLRSRGGSGI
Sbjct: 181 NLMNMCLIFLMEQVKADTVLIESDMVARAILDVIPILNIRKLVLGANKSRKLRSRGGSGI 240

Query: 241 ANEILQKAPAFCEVKVVCEGKETSQLGGSPSPLSSPRYQDDSFKDNDPNSTITVAEEQRN 283
           ANEILQKAP FCEVKVVCE KETSQLGG PSP+SSPRYQDDS KDNDPNSTITVAEEQRN
Sbjct: 241 ANEILQKAPEFCEVKVVCERKETSQLGGLPSPISSPRYQDDSSKDNDPNSTITVAEEQRN 300

BLAST of Sed0027781 vs. ExPASy Swiss-Prot
Match: Q8GUH1 (U-box domain-containing protein 33 OS=Arabidopsis thaliana OX=3702 GN=PUB33 PE=2 SV=2)

HSP 1 Score: 60.8 bits (146), Expect = 2.8e-08
Identity = 47/180 (26.11%), Postives = 85/180 (47.22%), Query Frame = 0

Query: 75  EDCVYVGVGRN-DSSFDALQWTLKNTITNSTTVVYLLHVFPETRYIPSPLGKIPINQVSK 134
           ++ ++V V ++   S   L W L+NT       + L+HV   ++ IP    K P+  V +
Sbjct: 44  DEKIFVAVDKHVAKSKSTLIWALQNT---GGKKICLIHVHQPSQMIPLMGAKFPVGAVKE 103

Query: 135 QQVAIHAAQEESKRKDFLQNFIDSCSAVKVKADTVLIESDIVARAILDVIPILNIRKLVL 194
           ++V +   +E  K    L +++  C    V+A+ + IE + +   I+ +I  L IRKLV+
Sbjct: 104 EEVRVFREKEREKVHMILDDYLRICQQRGVRAEKMFIEMESIENGIVQLISELGIRKLVM 163

Query: 195 GVNKSRKLRSRG---GSGIANEILQKAPAFCEVKVVCEG----KETSQLGGSPSPLSSPR 247
           G    R    R     S  A  + ++AP  C++   C+G       + +  + S  +SPR
Sbjct: 164 GAAADRHYSRRMTDLKSRKAIFVRREAPTLCQIWFTCKGYLIHTREATMDDTESEYASPR 220

BLAST of Sed0027781 vs. ExPASy Swiss-Prot
Match: Q9FKG6 (U-box domain-containing protein 52 OS=Arabidopsis thaliana OX=3702 GN=PUB52 PE=2 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 5.8e-06
Identity = 49/196 (25.00%), Postives = 86/196 (43.88%), Query Frame = 0

Query: 78  VYVGVGRNDSSFDALQWTLKNTITNSTTVVYLLHVFPETRYIPSPLG-KIPINQVSKQQV 137
           V V +     S   + W L+  I    T   LL+V P   YIP+P+G  + ++++ +  V
Sbjct: 24  VAVAINGKKKSKYVVFWALEKFIPEGFTDFKLLYVRPPVSYIPTPMGIAVAVSELREDVV 83

Query: 138 AIHAAQEESKRKDFLQNFIDSCSAVKVKADTVLIESDIVARAILDVIPILNIRKLVLGVN 197
           + +  + +    + L+ +       KV+ + +L++S   A AI + I    + KLV+G++
Sbjct: 84  SAYKQELDWSANEMLRPYKKMFERRKVQVEVLLLDSLEPAAAIAEEIAGTGVTKLVIGMS 143

Query: 198 K----SRKLRSRGGSGIANEILQKAPAFCEVKVVCEGK-------------------ETS 250
                SRK+       +++ I    P FC V V+ +GK                    +S
Sbjct: 144 LRGFFSRKI------DMSSLIATAVPRFCTVYVISKGKLASVRPSESDASGSIRFERSSS 203

BLAST of Sed0027781 vs. ExPASy Swiss-Prot
Match: Q683D5 (U-box domain-containing protein 37 OS=Arabidopsis thaliana OX=3702 GN=PUB37 PE=2 SV=1)

HSP 1 Score: 47.0 bits (110), Expect = 4.1e-04
Identity = 46/211 (21.80%), Postives = 88/211 (41.71%), Query Frame = 0

Query: 69  SDALKWEDCVYVGVGRNDSSFDALQWTLKNTITNSTTVVYLLHVFPETRYIPSPLGKIPI 128
           S  L  ++ +YV VG +  +   L W ++NT      +V          ++  PL +   
Sbjct: 7   SQQLLMDEKIYVAVGSDLGNKSTLVWAIQNTGGKEFCIV----------HVHQPLYR--- 66

Query: 129 NQVSKQQVAIHAAQEESKRKDFLQNFIDSCSAVKVKADTVLIESDIVARAILDVIPILNI 188
                        +E+ K +  L  ++  C  ++V A+ + I+ + V + I+ +I   N+
Sbjct: 67  -------------KEKEKTQKILDKYLQKCRQMQVCAEMIHIKMESVEKGIIQLISERNV 126

Query: 189 RKLVLGVNKSRKLRSRGGSGIANE---ILQKAPAFCEVKVVCEG----KETSQLGGSPSP 248
           +KLV+G     +   R    ++ +   I Q+APA C +   C+G    K  S +G +   
Sbjct: 127 KKLVMGAASDTRYSMRMADLLSTKAIYIRQEAPATCCIWFTCKGYLVYKRESIMGNTSLE 186

Query: 249 LSSPRYQDDSFKDND---PNSTITVAEEQRN 270
            +S     DS +      P+   T++    N
Sbjct: 187 YASTSSGQDSVRSRGSVIPSRQFTISRGNGN 191

BLAST of Sed0027781 vs. ExPASy Swiss-Prot
Match: Q9FGD7 (Putative U-box domain-containing protein 50 OS=Arabidopsis thaliana OX=3702 GN=PUB50 PE=3 SV=1)

HSP 1 Score: 45.8 bits (107), Expect = 9.2e-04
Identity = 39/159 (24.53%), Postives = 70/159 (44.03%), Query Frame = 0

Query: 78  VYVGVGRN-DSSFDALQWTLK--NTITNSTTVVYLLHVFPETRYIPSPLGKIPINQVSKQ 137
           VY+ VG +    +  + W LK  N I  S  +++L ++  +  ++ +P GK+P + VS++
Sbjct: 22  VYIAVGNDVQEGYKTIHWALKKWNNIPISIVLLHLCNISQD--FVYTPFGKLPASSVSEE 81

Query: 138 QVAIHAAQEESKRKDFLQNFIDSCSAVKVKADTVLIESDIVARAILDVIPILNIRKLVLG 197
           ++ +    E+ K    L  +I  C                       V P L++  ++ G
Sbjct: 82  KLQVLRKYEDQKIDKLLSKYITFCG---------------------KVCP-LSVNFVLFG 141

Query: 198 VNKSRKLRSRGGSGIANEILQKAPAFCEVKVVCEGKETS 234
            +KS    +  GS     + Q  P FCE  ++C GK  S
Sbjct: 142 KSKS----AISGSFY---VYQNKPEFCEFYIICGGKMVS 149

BLAST of Sed0027781 vs. ExPASy TrEMBL
Match: A0A6J1EFC8 (U-box domain-containing protein 35-like isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC111433795 PE=4 SV=1)

HSP 1 Score: 474.2 bits (1219), Expect = 3.9e-130
Identity = 248/289 (85.81%), Postives = 260/289 (89.97%), Query Frame = 0

Query: 1   MSTLPSPAEAGHFHPDEGLGSEFSNYRY-NSTSEIEEEGSAETFEIKRQMLSPA------ 60
           MS LPSPAEAG+FH D+G GSEFS YRY +STSEIEEE SA+TFEIKRQ +S A      
Sbjct: 1   MSALPSPAEAGNFHGDDGFGSEFSTYRYSSSTSEIEEEDSAQTFEIKRQTVSAAAAAGAG 60

Query: 61  MEISREDYSLGCSFSSDALKWEDCVYVGVGRNDSSFDALQWTLKNTITNSTTVVYLLHVF 120
           +E  REDYS GCSFSSDALKWEDCVYVGVG++DSS +ALQW LKN IT STTVVYLLHVF
Sbjct: 61  LESIREDYSRGCSFSSDALKWEDCVYVGVGKSDSSNEALQWALKNAITTSTTVVYLLHVF 120

Query: 121 PETRYIPSPLGKIPINQVSKQQVAIHAAQEESKRKDFLQNFIDSCSAVKVKADTVLIESD 180
           PETRYIPSPLGKIP+NQVSKQQVAIH AQEESKRKDFLQNFIDSCS  KVKADTVLIESD
Sbjct: 121 PETRYIPSPLGKIPVNQVSKQQVAIHVAQEESKRKDFLQNFIDSCSLAKVKADTVLIESD 180

Query: 181 IVARAILDVIPILNIRKLVLGVNKSRKLRSRGGSGIANEILQKAPAFCEVKVVCEGKETS 240
           +VARAILDVIPILNIRKLVLG NKSRKLRSRGGSGIANEILQKAP FCEVKVVCE KETS
Sbjct: 181 MVARAILDVIPILNIRKLVLGANKSRKLRSRGGSGIANEILQKAPEFCEVKVVCERKETS 240

Query: 241 QLGGSPSPLSSPRYQDDSFKDNDPNSTITVAEEQRNSSVSCMCFKTKFV 283
           QLGG PSP+SSPRYQDDS KDNDPNSTITVAEEQRNSSVSCMCFKTKFV
Sbjct: 241 QLGGLPSPISSPRYQDDSSKDNDPNSTITVAEEQRNSSVSCMCFKTKFV 289

BLAST of Sed0027781 vs. ExPASy TrEMBL
Match: A0A6J1KP30 (U-box domain-containing protein 35-like isoform X4 OS=Cucurbita maxima OX=3661 GN=LOC111496996 PE=4 SV=1)

HSP 1 Score: 469.2 bits (1206), Expect = 1.2e-128
Identity = 246/290 (84.83%), Postives = 260/290 (89.66%), Query Frame = 0

Query: 1   MSTLPSPAEAGHFHPDEGLGSEFSNYRYN-STSEIEEEGSAETFEIKRQMLSPA------ 60
           MS LPSPA AG+F  ++G GSEF+ YRYN STSEIEEE SA+TFEIKR+ +S A      
Sbjct: 1   MSALPSPAGAGNFRGNDGFGSEFNTYRYNSSTSEIEEEDSAQTFEIKRKTVSAAAAAAGA 60

Query: 61  -MEISREDYSLGCSFSSDALKWEDCVYVGVGRNDSSFDALQWTLKNTITNSTTVVYLLHV 120
            +E  REDYS GCSFSSDALKWEDCVYVGVG++DSS +ALQW LKN IT+STTVVYLLHV
Sbjct: 61  GLESIREDYSRGCSFSSDALKWEDCVYVGVGKSDSSNEALQWALKNAITSSTTVVYLLHV 120

Query: 121 FPETRYIPSPLGKIPINQVSKQQVAIHAAQEESKRKDFLQNFIDSCSAVKVKADTVLIES 180
           FPETRYIPSPLGKIPINQVSKQQVAIH AQEESKRKDFLQNFIDSCS  KVKADTVLIES
Sbjct: 121 FPETRYIPSPLGKIPINQVSKQQVAIHVAQEESKRKDFLQNFIDSCSLAKVKADTVLIES 180

Query: 181 DIVARAILDVIPILNIRKLVLGVNKSRKLRSRGGSGIANEILQKAPAFCEVKVVCEGKET 240
           D+VARAILDVIPILNIRKLVLG NKSRKLRSRGGSGIANEILQKAP FCEVKVVCEGKET
Sbjct: 181 DMVARAILDVIPILNIRKLVLGANKSRKLRSRGGSGIANEILQKAPEFCEVKVVCEGKET 240

Query: 241 SQLGGSPSPLSSPRYQDDSFKDNDPNSTITVAEEQRNSSVSCMCFKTKFV 283
           SQLGG PSP+SSPRYQDDS KDNDPNSTITVAEEQRNSSVSCMCFKTKFV
Sbjct: 241 SQLGGLPSPISSPRYQDDSSKDNDPNSTITVAEEQRNSSVSCMCFKTKFV 290

BLAST of Sed0027781 vs. ExPASy TrEMBL
Match: A0A6J1EM32 (uncharacterized protein LOC111433795 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111433795 PE=4 SV=1)

HSP 1 Score: 460.7 bits (1184), Expect = 4.4e-126
Identity = 248/313 (79.23%), Postives = 260/313 (83.07%), Query Frame = 0

Query: 1   MSTLPSPAEAGHFHPDEGLGSEFSNYRY-NSTSEIEEEGSAETFEIKRQMLSPA------ 60
           MS LPSPAEAG+FH D+G GSEFS YRY +STSEIEEE SA+TFEIKRQ +S A      
Sbjct: 1   MSALPSPAEAGNFHGDDGFGSEFSTYRYSSSTSEIEEEDSAQTFEIKRQTVSAAAAAGAG 60

Query: 61  MEISREDYSLGCSFSSDALKWEDCVYVGVGRNDSSFDALQWTLKNTITNSTTVVYLLHVF 120
           +E  REDYS GCSFSSDALKWEDCVYVGVG++DSS +ALQW LKN IT STTVVYLLHVF
Sbjct: 61  LESIREDYSRGCSFSSDALKWEDCVYVGVGKSDSSNEALQWALKNAITTSTTVVYLLHVF 120

Query: 121 PETRYIPSPLGKIPINQVSKQQVAIHAAQEESKRKDFLQNFIDSCSAVK----------- 180
           PETRYIPSPLGKIP+NQVSKQQVAIH AQEESKRKDFLQNFIDSCS  K           
Sbjct: 121 PETRYIPSPLGKIPVNQVSKQQVAIHVAQEESKRKDFLQNFIDSCSLAKVHNRKACPLFA 180

Query: 181 -------------VKADTVLIESDIVARAILDVIPILNIRKLVLGVNKSRKLRSRGGSGI 240
                        VKADTVLIESD+VARAILDVIPILNIRKLVLG NKSRKLRSRGGSGI
Sbjct: 181 NLMNMCLIFLMEQVKADTVLIESDMVARAILDVIPILNIRKLVLGANKSRKLRSRGGSGI 240

Query: 241 ANEILQKAPAFCEVKVVCEGKETSQLGGSPSPLSSPRYQDDSFKDNDPNSTITVAEEQRN 283
           ANEILQKAP FCEVKVVCE KETSQLGG PSP+SSPRYQDDS KDNDPNSTITVAEEQRN
Sbjct: 241 ANEILQKAPEFCEVKVVCERKETSQLGGLPSPISSPRYQDDSSKDNDPNSTITVAEEQRN 300

BLAST of Sed0027781 vs. ExPASy TrEMBL
Match: A0A6J1EFV0 (U-box domain-containing protein 35-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111433795 PE=4 SV=1)

HSP 1 Score: 459.5 bits (1181), Expect = 9.9e-126
Identity = 247/313 (78.91%), Postives = 260/313 (83.07%), Query Frame = 0

Query: 1   MSTLPSPAEAGHFHPDEGLGSEFSNYRY-NSTSEIEEEGSAETFEIKRQMLSPA------ 60
           MS LPSPAEAG+FH D+G GSEFS YRY +STSEIEEE SA+TFEIKRQ +S A      
Sbjct: 1   MSALPSPAEAGNFHGDDGFGSEFSTYRYSSSTSEIEEEDSAQTFEIKRQTVSAAAAAGAG 60

Query: 61  MEISREDYSLGCSFSSDALKWEDCVYVGVGRNDSSFDALQWTLKNTITNSTTVVYLLHVF 120
           +E  REDYS GCSFSSDALKWEDCVYVGVG++DSS +ALQW LKN IT STTVVYLLHVF
Sbjct: 61  LESIREDYSRGCSFSSDALKWEDCVYVGVGKSDSSNEALQWALKNAITTSTTVVYLLHVF 120

Query: 121 PETRYIPSP------------------------LGKIPINQVSKQQVAIHAAQEESKRKD 180
           PETRYIPSP                        +GKIP+NQVSKQQVAIH AQEESKRKD
Sbjct: 121 PETRYIPSPCNSLNSLFFDSFLSFNLCVVSVMTVGKIPVNQVSKQQVAIHVAQEESKRKD 180

Query: 181 FLQNFIDSCSAVKVKADTVLIESDIVARAILDVIPILNIRKLVLGVNKSRKLRSRGGSGI 240
           FLQNFIDSCS  KVKADTVLIESD+VARAILDVIPILNIRKLVLG NKSRKLRSRGGSGI
Sbjct: 181 FLQNFIDSCSLAKVKADTVLIESDMVARAILDVIPILNIRKLVLGANKSRKLRSRGGSGI 240

Query: 241 ANEILQKAPAFCEVKVVCEGKETSQLGGSPSPLSSPRYQDDSFKDNDPNSTITVAEEQRN 283
           ANEILQKAP FCEVKVVCE KETSQLGG PSP+SSPRYQDDS KDNDPNSTITVAEEQRN
Sbjct: 241 ANEILQKAPEFCEVKVVCERKETSQLGGLPSPISSPRYQDDSSKDNDPNSTITVAEEQRN 300

BLAST of Sed0027781 vs. ExPASy TrEMBL
Match: A0A6J1KRK4 (U-box domain-containing protein 35-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111496996 PE=4 SV=1)

HSP 1 Score: 455.7 bits (1171), Expect = 1.4e-124
Identity = 246/314 (78.34%), Postives = 260/314 (82.80%), Query Frame = 0

Query: 1   MSTLPSPAEAGHFHPDEGLGSEFSNYRYN-STSEIEEEGSAETFEIKRQMLSPA------ 60
           MS LPSPA AG+F  ++G GSEF+ YRYN STSEIEEE SA+TFEIKR+ +S A      
Sbjct: 1   MSALPSPAGAGNFRGNDGFGSEFNTYRYNSSTSEIEEEDSAQTFEIKRKTVSAAAAAAGA 60

Query: 61  -MEISREDYSLGCSFSSDALKWEDCVYVGVGRNDSSFDALQWTLKNTITNSTTVVYLLHV 120
            +E  REDYS GCSFSSDALKWEDCVYVGVG++DSS +ALQW LKN IT+STTVVYLLHV
Sbjct: 61  GLESIREDYSRGCSFSSDALKWEDCVYVGVGKSDSSNEALQWALKNAITSSTTVVYLLHV 120

Query: 121 FPETRYIPSPLGKIPINQVSKQQVAIHAAQEESKRKDFLQNFIDSCSAVK---------- 180
           FPETRYIPSPLGKIPINQVSKQQVAIH AQEESKRKDFLQNFIDSCS  K          
Sbjct: 121 FPETRYIPSPLGKIPINQVSKQQVAIHVAQEESKRKDFLQNFIDSCSLAKVHKRKACPLF 180

Query: 181 --------------VKADTVLIESDIVARAILDVIPILNIRKLVLGVNKSRKLRSRGGSG 240
                         VKADTVLIESD+VARAILDVIPILNIRKLVLG NKSRKLRSRGGSG
Sbjct: 181 ANLMNMCLIFLTEQVKADTVLIESDMVARAILDVIPILNIRKLVLGANKSRKLRSRGGSG 240

Query: 241 IANEILQKAPAFCEVKVVCEGKETSQLGGSPSPLSSPRYQDDSFKDNDPNSTITVAEEQR 283
           IANEILQKAP FCEVKVVCEGKETSQLGG PSP+SSPRYQDDS KDNDPNSTITVAEEQR
Sbjct: 241 IANEILQKAPEFCEVKVVCEGKETSQLGGLPSPISSPRYQDDSSKDNDPNSTITVAEEQR 300

BLAST of Sed0027781 vs. TAIR 10
Match: AT5G47740.1 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 169.5 bits (428), Expect = 3.9e-42
Identity = 106/255 (41.57%), Postives = 157/255 (61.57%), Query Frame = 0

Query: 31  TSEIEEEGSAETFEIKRQMLSPAMEISREDYSLGCSFSSDALKWEDCVYVGVGRNDSSFD 90
           ++EIEEE   E  + +R+     +E  RE    G S  + ++  ED VYVGVG+ DSS +
Sbjct: 5   SNEIEEEEEQE--DGRRR-----LESVRESQLEGRSSGTVSMNGEDNVYVGVGKGDSSME 64

Query: 91  ALQWTLKNTITNSTTVVYLLHVFPETRYIPSPLGKIPINQVSKQQVAIHAAQEESKRKDF 150
           AL+W + N +T+S+T+++L+HVFPETR+IP PLG+I   + S++QV    +QE  KR+  
Sbjct: 65  ALRWAIDNLMTSSSTLLFLIHVFPETRFIPYPLGRITRERASQEQVESFMSQEREKRRTL 124

Query: 151 LQNFIDSCSAVKVKADTVLIESDIVARAILDVIPILNIRKLVLGVNKS--RKLRSRGGSG 210
           L  F+ +CSA KVK +T+L+ESD VA+A+ D+I ILNIRKLVLG++KS  RK  +  G+ 
Sbjct: 125 LLKFLHACSASKVKVETILVESDSVAKAVQDLITILNIRKLVLGIDKSNARKASTMKGNS 184

Query: 211 IANEILQKAPA-FCEVKVVCEGKETS--QLGGSPSPLSSPRYQDDSFKDNDPNSTITVAE 270
           +   I++ + A  CEVKV+C+GKE +  Q     SP  SP  Q                +
Sbjct: 185 VPELIMRSSAADVCEVKVICQGKEINMEQTMMESSPAKSPIVQRQK-------------K 239

Query: 271 EQRNSSVSCMCFKTK 281
           +Q     +C+CF +K
Sbjct: 245 DQPVDPFACICFISK 239

BLAST of Sed0027781 vs. TAIR 10
Match: AT5G47740.2 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 164.5 bits (415), Expect = 1.3e-40
Identity = 106/257 (41.25%), Postives = 157/257 (61.09%), Query Frame = 0

Query: 31  TSEIEEEGSAETFEIKRQMLSPAMEISREDYSLGCSFSSDALKWEDCVYVGVGRNDSSFD 90
           ++EIEEE   E  + +R+     +E  RE    G S  + ++  ED VYVGVG+ DSS +
Sbjct: 5   SNEIEEEEEQE--DGRRR-----LESVRESQLEGRSSGTVSMNGEDNVYVGVGKGDSSME 64

Query: 91  ALQWTLKNTITNSTTVVYLLHVFPETRYIPSPLGKIPINQVSKQQVAIHAAQEESKRKDF 150
           AL+W + N +T+S+T+++L+HVFPETR+IP PLG+I   + S++QV    +QE  KR+  
Sbjct: 65  ALRWAIDNLMTSSSTLLFLIHVFPETRFIPYPLGRITRERASQEQVESFMSQEREKRRTL 124

Query: 151 LQNFIDSCSAVK--VKADTVLIESDIVARAILDVIPILNIRKLVLGVNKS--RKLRSRGG 210
           L  F+ +CSA K  VK +T+L+ESD VA+A+ D+I ILNIRKLVLG++KS  RK  +  G
Sbjct: 125 LLKFLHACSASKEQVKVETILVESDSVAKAVQDLITILNIRKLVLGIDKSNARKASTMKG 184

Query: 211 SGIANEILQKAPA-FCEVKVVCEGKETS--QLGGSPSPLSSPRYQDDSFKDNDPNSTITV 270
           + +   I++ + A  CEVKV+C+GKE +  Q     SP  SP  Q               
Sbjct: 185 NSVPELIMRSSAADVCEVKVICQGKEINMEQTMMESSPAKSPIVQRQK------------ 241

Query: 271 AEEQRNSSVSCMCFKTK 281
            ++Q     +C+CF +K
Sbjct: 245 -KDQPVDPFACICFISK 241

BLAST of Sed0027781 vs. TAIR 10
Match: AT5G65500.1 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 72.0 bits (175), Expect = 8.5e-13
Identity = 49/162 (30.25%), Postives = 82/162 (50.62%), Query Frame = 0

Query: 78  VYVGVGRN-DSSFDALQWTLK--NTITNSTTVVYLLHVFPETRYIPSPLGKIPINQVSKQ 137
           VY+ VG +    +  + W LK  N I  S  +++L ++  +  ++ +P GK+P + VS++
Sbjct: 22  VYIAVGNDVQEGYKTIHWALKKWNNIPISIVLLHLCNISQD--FVYTPFGKLPASSVSEE 81

Query: 138 QVAIHAAQEESKRKDFLQNFIDSCSAVKVKADTVLIESDIVARAILDVIPILNIRKLVLG 197
           ++ +    E+ K    L  +I  C  VK +   V  + D +   ILD+I  L I KLV+G
Sbjct: 82  KLQVLRKYEDQKIDKLLSKYITFCGKVKAELLKVEKQHDSIQVLILDLISKLRITKLVMG 141

Query: 198 VNKSRKLRS-RGGSGIANE--ILQKAPAFCEVKVVCEGKETS 234
           +   R   S +  S I+    + Q  P FCE  ++C GK  S
Sbjct: 142 ITFMRSSSSWKSKSAISGSFYVYQNKPEFCEFYIICGGKMVS 181

BLAST of Sed0027781 vs. TAIR 10
Match: AT2G45910.1 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 60.8 bits (146), Expect = 2.0e-09
Identity = 47/180 (26.11%), Postives = 85/180 (47.22%), Query Frame = 0

Query: 75  EDCVYVGVGRN-DSSFDALQWTLKNTITNSTTVVYLLHVFPETRYIPSPLGKIPINQVSK 134
           ++ ++V V ++   S   L W L+NT       + L+HV   ++ IP    K P+  V +
Sbjct: 44  DEKIFVAVDKHVAKSKSTLIWALQNT---GGKKICLIHVHQPSQMIPLMGAKFPVGAVKE 103

Query: 135 QQVAIHAAQEESKRKDFLQNFIDSCSAVKVKADTVLIESDIVARAILDVIPILNIRKLVL 194
           ++V +   +E  K    L +++  C    V+A+ + IE + +   I+ +I  L IRKLV+
Sbjct: 104 EEVRVFREKEREKVHMILDDYLRICQQRGVRAEKMFIEMESIENGIVQLISELGIRKLVM 163

Query: 195 GVNKSRKLRSRG---GSGIANEILQKAPAFCEVKVVCEG----KETSQLGGSPSPLSSPR 247
           G    R    R     S  A  + ++AP  C++   C+G       + +  + S  +SPR
Sbjct: 164 GAAADRHYSRRMTDLKSRKAIFVRREAPTLCQIWFTCKGYLIHTREATMDDTESEYASPR 220

BLAST of Sed0027781 vs. TAIR 10
Match: AT5G61550.1 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 53.1 bits (126), Expect = 4.1e-07
Identity = 49/196 (25.00%), Postives = 86/196 (43.88%), Query Frame = 0

Query: 78  VYVGVGRNDSSFDALQWTLKNTITNSTTVVYLLHVFPETRYIPSPLG-KIPINQVSKQQV 137
           V V +     S   + W L+  I    T   LL+V P   YIP+P+G  + ++++ +  V
Sbjct: 24  VAVAINGKKKSKYVVFWALEKFIPEGFTDFKLLYVRPPVSYIPTPMGIAVAVSELREDVV 83

Query: 138 AIHAAQEESKRKDFLQNFIDSCSAVKVKADTVLIESDIVARAILDVIPILNIRKLVLGVN 197
           + +  + +    + L+ +       KV+ + +L++S   A AI + I    + KLV+G++
Sbjct: 84  SAYKQELDWSANEMLRPYKKMFERRKVQVEVLLLDSLEPAAAIAEEIAGTGVTKLVIGMS 143

Query: 198 K----SRKLRSRGGSGIANEILQKAPAFCEVKVVCEGK-------------------ETS 250
                SRK+       +++ I    P FC V V+ +GK                    +S
Sbjct: 144 LRGFFSRKI------DMSSLIATAVPRFCTVYVISKGKLASVRPSESDASGSIRFERSSS 203