Sed0027695 (gene) Chayote v1

Overview


Name	Sed0027695
Type	gene
Organism	Sechium edule (Chayote v1)
Description	Nitrilase/cyanide hydratase
Location	LG11: 27774633 .. 27780270 (+)
RNA-Seq Expression	Sed0027695
Synteny	Sed0027695

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CAATACTGACGGTACTGTCTCTGTCTTTTCCTTCCCTTGAGTGCGCACAACCAAACATCCCGACCGTGAATATCAACTCGACTATGGCCCAGAAAAGGCAATATACTTTTCTAGCAACTTCCACCAATTCGCTCTATTAATACATCATTTTTTTTCATTTAAAAAAAGAAATCCGAATCCACAGCAGCCAGCCATTGATCGCACAGTAACCACATTCCAAGCTCAAGCTCAAGCTCAGGGAGATTGAGAATCACCTACCATGGCCGCGAACGATTTACAGCTGATACCGGAGGTGGATATGGGCTCCGATTCGTCTGTTCCTACCACTGTTCGAGCCACCGTCGTCCAGGCCTCCACCATCTTCTACGACACTCCGGCTACTCTAGGTTTTCCTTTCTTTTCCATCGCTTCCACTTTTGATTTCATTCGATTTGATTTGCTTGTTATGGAATACTACTTCTCTCCCTGTTTCATTCATTCATTCTTCCAGATTGCTTTTGCTGTGTTCTTAGAAGCTTATTAGTTCTTTGGTTCGGCTTTTCTTTTTTTCTTTTTATTATAATTTTTTGTATCTTTTATGTGCGATGAATGATTGAATTAGTTTAAGCTACTACATCCTCCAATTGTTTCCAGGTTTTTTTTAAATTGATTCTCTTGTCTGTGATCACTATGAATTTGGTAATGTCGCCTAATGTAAGCCTTCTGATTGTTCTGTTCCTCTGAGTTTCGGTTAATTTGAGAAATAGAAGAGAATGCGTACACATGAATGATGGCGGCTTAGAAATTTAGTGATATTTTTCTATGTGGAAGATCTTTGAGTTTTTCAACTCCTAAAGCCATGGGGATTATCACTAGTAGATGAAAAATGTTTTAGCATATTTATGAGCTACCTGTTAGGATCCCACCAAATTGATTTTGAGATGAATATGAATGTCATGTATATTTAATATAGTATCAGAGCTTATTTAGCTTAAGTGGGTATTCAGCAAAATAAAGTTTGGATCCGATTCAAGATTCGTGAAATCCAAAAGACCCACCATCTTGAGGGACAAGACATGTTAAGATATCACTATTGTCACACGTTGGAAAAGTAGAGAGTTCTCCAGATAGATGAACTACTTGTCTTATCGCTAATTGGTTTTTCAGACGGATGTCCACATTATCTAATATTAGCTACCGGTCTATGACCATGCTGGTGTTTACTGTTTTCTATTCTTTTGGTTTTGGTTGGTAAAACAAAATCTTATTCTGATTTACATGAACAGATAAGGCTGAGAAGTTGTTGGCTGAAGCGGCTGGATTTGGTTCACAACTAGTTGTATTTCCAGAAGCCTTTGTTGGGGGTTATCCCCGTGGATCAAATTTTGGTGTTTCCATTGGGAATAGAACTGCAAAGGGGAAAGAGGAGTTTCGTAAATATCATGCAGCAGCAATTGATGTACCTGGTTAGTCCTTGTTTCCAGATCAACTTTTGTTGTTTGACTTTATAGTTAATTATGCACATGTAAATGGAAGAGTCTTAGTTACTGTGGGTAAATTCAGGTTCAATGGACGTTGTTAGGTTCTTACCTTAATTGAATAATATTAGTACTCATTCTAGGAGGCTGACTTTGTTTCTCCATCAGTCTCTACCAAACTTTATTATCTAGTCATATGCTAATGCTAATATTCAAGTCTGAAATCATAATTTACATCTTCTGCTCTTATCATCCTTCAATTTTTCAATTGAATTTAGTTCATATTAGGACAGTACAATAAGGGGGAGTTGGTGATGTTAGTCTTCTTAATCTGAAACTCAACGAGTCAGTTTTCTCATAATTTATTGTCAAACCATTCTTATATATTTGTAAGTCACATTAAAAAAAGAAAATGTCGGAAGTGCATTAAGTGTAGTTCTACTAGAGTAATAAATAATATTTTGACATCTATAAATGGTGATATATGCAGGCCCTGAAGTTGATCGATTGGCAGCAATGGCTGGAAAGTATAAGGTACATTTGGTAATGGGGGTGATAGAGAGAGATGGCTACACACTGTACTGTACTGTTCTATTTTTTGATCCTCAAGGCCATTACTTGGGTAAGCACAGGAAAGTGATGCCAACTGCATTGGAGCGGATAATCTGGGGCTTTGGTGATGGATCAACAATTCCTGTTTTTGAGACTACAATTGGAAAGATAGGTGCTGCCATTTGTTGGGAAAACAGAATGCCACTCCTAAGAACAGCTATGTATGCCAAAGGTTAGCTTTTCTTTGCAGGGAATGATCAAATTTTTTACCTTCAACCGCTTATTTGTAATTTCAGACATCAATGCAATTGTTTCTTATCCGAAAAATAATAAATAAACCCGTGGAGTTTACAAATTCGAAGTTGATCACTAGAAACTGACTTATATTTATGTTCATGTATGTAGGCATTGAAATATATTGTGCACCAACTGCTGATTCCCGGGATACATGGCAAGCATCTATGACCCACATTGCTTTGGAGGGTGGATGTTTTGTTTTGTCTGCGAACCAGTTCTGCCGAAGAAAAGACTACCCACCTCCACCTGAATATGTTTTTAGTGGCACAGAAGAGGAACTCAGCCCAGATTCTGTTGTTTGTGCTGGGGGTAGTGTCATCATATCACCGTCAGGGACAATCCTAGCTGGACCGAATTATGATGGGGAGGCACTTATTTCAGCAGATCTAGGTATTTCATCACGTCCTTCTGCTTATAAACATCTGAGGTATAGTTATGTACTCCCGGTAATTATCATGTTGAGAAGAAACAAATAAACGCCCACTGTGGGGTTCGAACCTAGGACCATAAGGTTAGAGTCGTTTTATATGAATCGTGTTTAGTGATTGACGATTCATTTAAAAGTGTTTGATCTTTAGAAAACCTTCTATTAGATTTAAGCGTTATTCTTTTTCAAGAAAGTCATAGGGCTTAGTCTCCTAGGGGCTGTTTGTTTGTCATATTTAAAGAGGTTTTAGTTGGAGGATTTATTAACTCCTCCTCGAAAACTTACATCAAAACGAGTTTTTACTAACTCCTCCTTTTTACTTTTAAAAATAACTCTTATTTCTAACTATTCATTTCTAATCATTCATTTATAACTACTCAATCATTTCTAATCACTCATTTTTAATCATTCATTTCAAATCAATCAAAATAACTTATTTTTAACCACAATTTTTTGAACTACTTATTTTAAATTTACATCCAAACAAGCTTTTGACTCTAATTCCTCTACACAAAAACCACATTCAAACAAGTTTTTGACTACAACTCTCAATTTTTAACTACTCTAAATAACTCTTCAATATAACTCCTAAAGATAAATACTCACTAAATATGGTAAACAAACACCCCCCTAGATGATCAAATGGTTCTCATAGTCAATGAGTCTGAAACCTTCATTCCGATCTCTTATTCTATGTTTTATCTCCTTTTAGTTCCTTTATAGAGTGAATGGCTCGGCTCTCCTCGTACAATTTGGTTTTTCTGTATTCAATTTCAAATTAAGCGAGGAGGTTAGGATAAATAAACTGAGGTGGTTTTAATTAATCTAACTTTTGAGGCGTTACCAATTCGAGACATCTGGATTATTTATGACCTTAAAAGGCAATTTTCAGTGGGAGTATTTTGCAATCGTTGGACAAAGTATTCTTGAATAAAGTTAGCCTCATCAACTATTTTCTTTCAAACAATTTTTTTTTGTCCTCCTATCTTCCTTCTTGAGTTCAACGATAGTTGGACATGGGAGATTCGAGTTCATGACATTTTGATTCGTGGGCATGCGCTTAAGCTCTTCTTGTTGGCATCCTCTTATCTTTTTTTATTCTTAAAACTTGAGGATATGGGAGGGATAGAATGATGTAAGAAGAATAAGGTCGATTGGGGTCATTTCTTGATTAATCCTCTTTTAGTATTTTTTTTTTTTTTATACTTTTGGATTTTAGTTAGCTCTCTAATTCCCTATAAATAGGGAGTCCTCTCCCATGTATTGGACAATTATCATTCTAAGAATTATTCTAGAGTTTTTTCTGTGAGAATTATCTCCTTTTTATCATTTTAGACTAAGACTTCTACCCTCCTAAGTAAGTCACTAATACCAGCCACTAATTTTCTTTTAACGAGATTAGTCTTCAAGCCCAAACATTTTCTGAATAGAATAAAAGATTGAAGACAATAAAATCTCCCAAGCAAAAAGGACAGTATTATCTGTAAACGAAGGTTGGAGAGATGAACCAAGTACCAATAAATTTTACTTTTTATGGAATTTTAGGATTAGCCACCTCGATAATAATAGTTACCATTTTTAAATAAGCTTTTTCAATGTGCTATTCTCCCAAGGCTATAGACTTTTAATTAAGAACCTATAATAAGTTCTATCTCAGAATGGAGTAAAATATTTTGAAAATACTTCTGCTACCAAAAACCCACTTTTGACTAGTTAAAATGCCTAGAGTAAAAGACACTTGAATTTTAATTTATTTTCCTTAAACTTGGTCTTTGTTATCACATGTCTTGTTTATGAATGTTGAAATACGTTGTTTGAAACAATAGTAAACCAATGTTCTTTGGCACGGTGCAGTAGGAATTACTATTGATCCCTTCAAACTCCTAACAAGTTAAACTTTTTGGCCATCGTGAGTATAGTTCAACAGTAATTAACACGCTTTCCTTCTTATTACCAAAAATTGACACGTTTTGCTTCTTCGAGGTTAGAGGTTCAAAGTCCCACTCCATCTTTGTTATACTTGAAACAAAAGGAAACTCGACTTTTGTTTAGCATATTTTTTATTGCCTCCTTGAAATTGATTTGACTATTTTAAAAGGAACACTAATTTTTAGAATGAATAATAAACAGATCTCGGAGATATAGCGCGGGCAAAATTTGATTTTGATGTGGTTGGTCATTATGCAAGGCCTGAAGTGCTTAGCTTGGTTGTGCGGGACCATCCTACACCACCAGCTACTTTCACATCTACATCTTCAAAAACGGAAGGTTTTTGTAAAAATTAGTATGCAAACTGCTTCAATTTAATTCCATCAAAAGTTGTAGCTTGTATGTGTTCTCTGTTTTACAGTTTCATCTGGGTTCGGTGTTGTATTGCTATTATATATCAAGACACTTTAATATGTTGTAATTTCTTTTATTATTTTACTTTCTATTTCCTTTACTTTTCTGCCATAAGGGGTTGGTAGTTTTTATTGCTCACGTCTCTCTCTAAAAATTCTCTCAAGAAAGCATTACTCTGAAAAACCCCTTCTTTTGGCCACCCACAATAGCACGGCATTACGCTCTTTCCATTCAGTTTCTTCCTCCACCACGTTCAAGTATGCACGAAGTCTCTCATCCAACGAGAGATTTCACGACACGCGCTCATTTCTCGTCGGCAACTACAATCGTGTGCATTATCCCAAGCACCCACACAAAGCGAAAACTCTTGGCAACAATGAGTGGGTCATCACTAGTGCTCGAATCTACTGCTAGCGACTGACTCGCAAAATGTTCAACTTTCTAAGAGCTACAGTTTTGTTTTATTTTCCATGTTATCTTATTGTAATAAAAGCTCAGAACCTGGTGCTCCTCTTTGTAACAATTTCATTTTATCAATAAAGAGGTTTTTCTCCGACTAAAA

mRNA sequence

CAATACTGACGGTACTGTCTCTGTCTTTTCCTTCCCTTGAGTGCGCACAACCAAACATCCCGACCGTGAATATCAACTCGACTATGGCCCAGAAAAGGCAATATACTTTTCTAGCAACTTCCACCAATTCGCTCTATTAATACATCATTTTTTTTCATTTAAAAAAAGAAATCCGAATCCACAGCAGCCAGCCATTGATCGCACAGTAACCACATTCCAAGCTCAAGCTCAAGCTCAGGGAGATTGAGAATCACCTACCATGGCCGCGAACGATTTACAGCTGATACCGGAGGTGGATATGGGCTCCGATTCGTCTGTTCCTACCACTGTTCGAGCCACCGTCGTCCAGGCCTCCACCATCTTCTACGACACTCCGGCTACTCTAGATAAGGCTGAGAAGTTGTTGGCTGAAGCGGCTGGATTTGGTTCACAACTAGTTGTATTTCCAGAAGCCTTTGTTGGGGGTTATCCCCGTGGATCAAATTTTGGTGTTTCCATTGGGAATAGAACTGCAAAGGGGAAAGAGGAGTTTCGTAAATATCATGCAGCAGCAATTGATGTACCTGGCCCTGAAGTTGATCGATTGGCAGCAATGGCTGGAAAGTATAAGGTACATTTGGTAATGGGGGTGATAGAGAGAGATGGCTACACACTGTACTGTACTGTTCTATTTTTTGATCCTCAAGGCCATTACTTGGGTAAGCACAGGAAAGTGATGCCAACTGCATTGGAGCGGATAATCTGGGGCTTTGGTGATGGATCAACAATTCCTGTTTTTGAGACTACAATTGGAAAGATAGGTGCTGCCATTTGTTGGGAAAACAGAATGCCACTCCTAAGAACAGCTATGTATGCCAAAGGCATTGAAATATATTGTGCACCAACTGCTGATTCCCGGGATACATGGCAAGCATCTATGACCCACATTGCTTTGGAGGGTGGATGTTTTGTTTTGTCTGCGAACCAGTTCTGCCGAAGAAAAGACTACCCACCTCCACCTGAATATGTTTTTAGTGGCACAGAAGAGGAACTCAGCCCAGATTCTGTTGTTTGTGCTGGGGGTAGTGTCATCATATCACCGTCAGGGACAATCCTAGCTGGACCGAATTATGATGGGGAGGCACTTATTTCAGCAGATCTAGATCTCGGAGATATAGCGCGGGCAAAATTTGATTTTGATGTGGTTGGTCATTATGCAAGGCCTGAAGTGCTTAGCTTGGTTGTGCGGGACCATCCTACACCACCAGCTACTTTCACATCTACATCTTCAAAAACGGAAGGTTTTTGTAAAAATTAGTATGCAAACTGCTTCAATTTAATTCCATCAAAAGTTGTAGCTTGTATGTGTTCTCTGTTTTACAGTTTCATCTGGGTTCGGTGTTGTATTGCTATTATATATCAAGACACTTTAATATGTTGTAATTTCTTTTATTATTTTACTTTCTATTTCCTTTACTTTTCTGCCATAAGGGGTTGGTAGTTTTTATTGCTCACGTCTCTCTCTAAAAATTCTCTCAAGAAAGCATTACTCTGAAAAACCCCTTCTTTTGGCCACCCACAATAGCACGGCATTACGCTCTTTCCATTCAGTTTCTTCCTCCACCACGTTCAAGTATGCACGAAGTCTCTCATCCAACGAGAGATTTCACGACACGCGCTCATTTCTCGTCGGCAACTACAATCGTGTGCATTATCCCAAGCACCCACACAAAGCGAAAACTCTTGGCAACAATGAGTGGGTCATCACTAGTGCTCGAATCTACTGCTAGCGACTGACTCGCAAAATGTTCAACTTTCTAAGAGCTACAGTTTTGTTTTATTTTCCATGTTATCTTATTGTAATAAAAGCTCAGAACCTGGTGCTCCTCTTTGTAACAATTTCATTTTATCAATAAAGAGGTTTTTCTCCGACTAAAA

Coding sequence (CDS)

ATGGCCGCGAACGATTTACAGCTGATACCGGAGGTGGATATGGGCTCCGATTCGTCTGTTCCTACCACTGTTCGAGCCACCGTCGTCCAGGCCTCCACCATCTTCTACGACACTCCGGCTACTCTAGATAAGGCTGAGAAGTTGTTGGCTGAAGCGGCTGGATTTGGTTCACAACTAGTTGTATTTCCAGAAGCCTTTGTTGGGGGTTATCCCCGTGGATCAAATTTTGGTGTTTCCATTGGGAATAGAACTGCAAAGGGGAAAGAGGAGTTTCGTAAATATCATGCAGCAGCAATTGATGTACCTGGCCCTGAAGTTGATCGATTGGCAGCAATGGCTGGAAAGTATAAGGTACATTTGGTAATGGGGGTGATAGAGAGAGATGGCTACACACTGTACTGTACTGTTCTATTTTTTGATCCTCAAGGCCATTACTTGGGTAAGCACAGGAAAGTGATGCCAACTGCATTGGAGCGGATAATCTGGGGCTTTGGTGATGGATCAACAATTCCTGTTTTTGAGACTACAATTGGAAAGATAGGTGCTGCCATTTGTTGGGAAAACAGAATGCCACTCCTAAGAACAGCTATGTATGCCAAAGGCATTGAAATATATTGTGCACCAACTGCTGATTCCCGGGATACATGGCAAGCATCTATGACCCACATTGCTTTGGAGGGTGGATGTTTTGTTTTGTCTGCGAACCAGTTCTGCCGAAGAAAAGACTACCCACCTCCACCTGAATATGTTTTTAGTGGCACAGAAGAGGAACTCAGCCCAGATTCTGTTGTTTGTGCTGGGGGTAGTGTCATCATATCACCGTCAGGGACAATCCTAGCTGGACCGAATTATGATGGGGAGGCACTTATTTCAGCAGATCTAGATCTCGGAGATATAGCGCGGGCAAAATTTGATTTTGATGTGGTTGGTCATTATGCAAGGCCTGAAGTGCTTAGCTTGGTTGTGCGGGACCATCCTACACCACCAGCTACTTTCACATCTACATCTTCAAAAACGGAAGGTTTTTGTAAAAATTAG

Protein sequence

MAANDLQLIPEVDMGSDSSVPTTVRATVVQASTIFYDTPATLDKAEKLLAEAAGFGSQLVVFPEAFVGGYPRGSNFGVSIGNRTAKGKEEFRKYHAAAIDVPGPEVDRLAAMAGKYKVHLVMGVIERDGYTLYCTVLFFDPQGHYLGKHRKVMPTALERIIWGFGDGSTIPVFETTIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRKDYPPPPEYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLDLGDIARAKFDFDVVGHYARPEVLSLVVRDHPTPPATFTSTSSKTEGFCKN

Homology

[Close]

BLAST of Sed0027695 vs. NCBI nr
Match: XP_022927562.1 (bifunctional nitrilase/nitrile hydratase NIT4A [Cucurbita moschata])

HSP 1 Score: 664.5 bits (1713), Expect = 5.1e-187
Identity = 326/344 (94.77%), Postives = 332/344 (96.51%), Query Frame = 0


Query: 2   AANDLQLIPEVDMGSDSSVPTTVRATVVQASTIFYDTPATLDKAEKLLAEAAGFGSQLVV 61
           AA D QLI EVDMGSD SVP TVRATVVQASTIFYDTPATLDKAE+LLAEAAGFGSQLVV
Sbjct: 8   AAADTQLIAEVDMGSDVSVP-TVRATVVQASTIFYDTPATLDKAERLLAEAAGFGSQLVV 67

Query: 62  FPEAFVGGYPRGSNFGVSIGNRTAKGKEEFRKYHAAAIDVPGPEVDRLAAMAGKYKVHLV 121
           FPEAFVGGYPRGSNFGVSIGNRT KGKEEFRKYHAAAIDVPGPEVDRLAAMAGKY+VHLV
Sbjct: 68  FPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGKYRVHLV 127

Query: 122 MGVIERDGYTLYCTVLFFDPQGHYLGKHRKVMPTALERIIWGFGDGSTIPVFETTIGKIG 181
           MGVIERDGYTLYCTVLFFDPQG YLGKHRKVMPTALERIIWGFGDGSTIPVFET+IGKIG
Sbjct: 128 MGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFETSIGKIG 187

Query: 182 AAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRK 241
           AAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRK
Sbjct: 188 AAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRK 247

Query: 242 DYPPPPEYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLDLGDIAR 301
           DYPPPP+YVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLDLGDIAR
Sbjct: 248 DYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLDLGDIAR 307

Query: 302 AKFDFDVVGHYARPEVLSLVVRDHPTPPATFTSTSSKTEGFCKN 346
           AKFDFDVVGHYARPEVLSLVVRDHPT P TFTS ++KTE  CKN
Sbjct: 308 AKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSATAKTEDSCKN 350

[Close]

BLAST of Sed0027695 vs. NCBI nr
Match: XP_023520715.1 (bifunctional nitrilase/nitrile hydratase NIT4A [Cucurbita pepo subsp. pepo])

HSP 1 Score: 664.1 bits (1712), Expect = 6.7e-187
Identity = 326/343 (95.04%), Postives = 331/343 (96.50%), Query Frame = 0


Query: 2   AANDLQLIPEVDMGSDSSVPTTVRATVVQASTIFYDTPATLDKAEKLLAEAAGFGSQLVV 61
           AA D QLI EVDMGSD SVP TVRATVVQASTIFYDTPATLDKAE+LLAEAAGFGSQLVV
Sbjct: 8   AATDTQLIAEVDMGSDVSVP-TVRATVVQASTIFYDTPATLDKAERLLAEAAGFGSQLVV 67

Query: 62  FPEAFVGGYPRGSNFGVSIGNRTAKGKEEFRKYHAAAIDVPGPEVDRLAAMAGKYKVHLV 121
           FPEAFVGGYPRGSNFGVSIGNRT KGKEEFRKYHAAAIDVPGPEVDRLAAMAGKY+VHLV
Sbjct: 68  FPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGKYRVHLV 127

Query: 122 MGVIERDGYTLYCTVLFFDPQGHYLGKHRKVMPTALERIIWGFGDGSTIPVFETTIGKIG 181
           MGVIERDGYTLYCTVLFFDPQG YLGKHRKVMPTALERIIWGFGDGSTIPVFET+IGKIG
Sbjct: 128 MGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFETSIGKIG 187

Query: 182 AAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRK 241
           AAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRK
Sbjct: 188 AAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRK 247

Query: 242 DYPPPPEYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLDLGDIAR 301
           DYPPPP+YVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLDLGDIAR
Sbjct: 248 DYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLDLGDIAR 307

Query: 302 AKFDFDVVGHYARPEVLSLVVRDHPTPPATFTSTSSKTEGFCK 345
           AKFDFDVVGHYARPEVLSLVVRDHPT P TFTS S+KTE  CK
Sbjct: 308 AKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSASTKTEDSCK 349

[Close]

BLAST of Sed0027695 vs. NCBI nr
Match: XP_022973146.1 (bifunctional nitrilase/nitrile hydratase NIT4A [Cucurbita maxima])

HSP 1 Score: 664.1 bits (1712), Expect = 6.7e-187
Identity = 326/342 (95.32%), Postives = 330/342 (96.49%), Query Frame = 0


Query: 2   AANDLQLIPEVDMGSDSSVPTTVRATVVQASTIFYDTPATLDKAEKLLAEAAGFGSQLVV 61
           AAND QLI EVDMGSD SVP TVRATVVQASTIFYDTPATLDKAE+LLAEAAGFGSQLVV
Sbjct: 8   AANDTQLIAEVDMGSDVSVP-TVRATVVQASTIFYDTPATLDKAERLLAEAAGFGSQLVV 67

Query: 62  FPEAFVGGYPRGSNFGVSIGNRTAKGKEEFRKYHAAAIDVPGPEVDRLAAMAGKYKVHLV 121
           FPEAFVGGYPRGSNFGVSIGNRT KGKEEFRKYHAAAIDVPGPEVDRLAAMAGKY+VHLV
Sbjct: 68  FPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGKYRVHLV 127

Query: 122 MGVIERDGYTLYCTVLFFDPQGHYLGKHRKVMPTALERIIWGFGDGSTIPVFETTIGKIG 181
           MGVIERDGYTLYCTVLFFDPQG YLGKHRKVMPTALERIIWGFGDGSTIPVFET+IGKIG
Sbjct: 128 MGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFETSIGKIG 187

Query: 182 AAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRK 241
           AAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRK
Sbjct: 188 AAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRK 247

Query: 242 DYPPPPEYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLDLGDIAR 301
           DYPPPP+YVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLDLGDIAR
Sbjct: 248 DYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLDLGDIAR 307

Query: 302 AKFDFDVVGHYARPEVLSLVVRDHPTPPATFTSTSSKTEGFC 344
           AKFDFDVVGHYARPEVLSLVVRDHPT P TFTS S KTE  C
Sbjct: 308 AKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSASKKTEDSC 348

[Close]

BLAST of Sed0027695 vs. NCBI nr
Match: KAG6583706.1 (Bifunctional nitrilase/nitrile hydratase NIT4A, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019353.1 Bifunctional nitrilase/nitrile hydratase NIT4A [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 661.8 bits (1706), Expect = 3.3e-186
Identity = 324/343 (94.46%), Postives = 331/343 (96.50%), Query Frame = 0


Query: 2   AANDLQLIPEVDMGSDSSVPTTVRATVVQASTIFYDTPATLDKAEKLLAEAAGFGSQLVV 61
           AA D QLI EVDMGSD SVP TVRATVVQASTIFYDTPATLDKAE+LLAEAAGFGSQLV+
Sbjct: 8   AATDTQLIAEVDMGSDVSVP-TVRATVVQASTIFYDTPATLDKAERLLAEAAGFGSQLVL 67

Query: 62  FPEAFVGGYPRGSNFGVSIGNRTAKGKEEFRKYHAAAIDVPGPEVDRLAAMAGKYKVHLV 121
           FPEAFVGGYPRGSNFGVSIGNRT KGKEEFRKYHAAAIDVPGPEVDRLAAMAGKY+VHLV
Sbjct: 68  FPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGKYRVHLV 127

Query: 122 MGVIERDGYTLYCTVLFFDPQGHYLGKHRKVMPTALERIIWGFGDGSTIPVFETTIGKIG 181
           MGVIERDGYTLYCTVLFFDPQG YLGKHRKVMPTALERIIWGFGDGSTIPVFET+IGKIG
Sbjct: 128 MGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFETSIGKIG 187

Query: 182 AAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRK 241
           AAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRK
Sbjct: 188 AAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRK 247

Query: 242 DYPPPPEYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLDLGDIAR 301
           DYPPPP+YVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLDLGDIAR
Sbjct: 248 DYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLDLGDIAR 307

Query: 302 AKFDFDVVGHYARPEVLSLVVRDHPTPPATFTSTSSKTEGFCK 345
           AKFDFDVVGHYARPEVLSLVVRDHPT P TFTS ++KTE  CK
Sbjct: 308 AKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSATTKTEDSCK 349

[Close]

BLAST of Sed0027695 vs. NCBI nr
Match: XP_004139500.1 (bifunctional nitrilase/nitrile hydratase NIT4A [Cucumis sativus] >KGN64959.1 hypothetical protein Csa_022889 [Cucumis sativus])

HSP 1 Score: 654.8 bits (1688), Expect = 4.0e-184
Identity = 321/343 (93.59%), Postives = 328/343 (95.63%), Query Frame = 0


Query: 2   AANDLQLIPEVDMGSDSSVPTTVRATVVQASTIFYDTPATLDKAEKLLAEAAGFGSQLVV 61
           A  D QLI EVDMGSDSSV   VRATVVQASTIFYDTPATLDKAE+LLAEAAGFGSQLVV
Sbjct: 8   APTDSQLIAEVDMGSDSSV-MNVRATVVQASTIFYDTPATLDKAERLLAEAAGFGSQLVV 67

Query: 62  FPEAFVGGYPRGSNFGVSIGNRTAKGKEEFRKYHAAAIDVPGPEVDRLAAMAGKYKVHLV 121
           FPEAFVGGYPRGSNFGVSIGNRT KGKEEFRKYHA+AIDVPGPEVDRLAAMAGKY+VHLV
Sbjct: 68  FPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHASAIDVPGPEVDRLAAMAGKYRVHLV 127

Query: 122 MGVIERDGYTLYCTVLFFDPQGHYLGKHRKVMPTALERIIWGFGDGSTIPVFETTIGKIG 181
           MGVIERDGYTLYCTVLFFDPQG YLGKHRKVMPTALERIIWGFGDGSTIPVFET+IGKIG
Sbjct: 128 MGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFETSIGKIG 187

Query: 182 AAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRK 241
           AAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRK
Sbjct: 188 AAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRK 247

Query: 242 DYPPPPEYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLDLGDIAR 301
           DYPPPPEYVFSGTEEEL+PDSVVCAGGS IISPSGTILAGPNYDGEALISADLDLG+IAR
Sbjct: 248 DYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLDLGEIAR 307

Query: 302 AKFDFDVVGHYARPEVLSLVVRDHPTPPATFTSTSSKTEGFCK 345
           AKFDFDVVGHYARPEVLSLVVRDHPT P TFTSTS+K E  CK
Sbjct: 308 AKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKVEDSCK 349

[Close]

BLAST of Sed0027695 vs. ExPASy Swiss-Prot
Match: Q42965 (Bifunctional nitrilase/nitrile hydratase NIT4A OS=Nicotiana tabacum OX=4097 GN=NIT4A PE=2 SV=1)

HSP 1 Score: 617.5 bits (1591), Expect = 9.4e-176
Identity = 298/339 (87.91%), Postives = 318/339 (93.81%), Query Frame = 0


Query: 2   AANDLQLIPEVDMGSDSSVPTTVRATVVQASTIFYDTPATLDKAEKLLAEAAGFGSQLVV 61
           A N+  L  EVDMG +SS P TVRATVVQASTIFYDTPATL KAE+LLAEAA +G+QLVV
Sbjct: 8   AVNEGPLFAEVDMGDNSSTP-TVRATVVQASTIFYDTPATLVKAERLLAEAASYGAQLVV 67

Query: 62  FPEAFVGGYPRGSNFGVSIGNRTAKGKEEFRKYHAAAIDVPGPEVDRLAAMAGKYKVHLV 121
           FPEAF+GGYPRGS FGVSIGNRTAKGKEEFRKYHA+AIDVPGPEVDRLAAMAGKYKV+LV
Sbjct: 68  FPEAFIGGYPRGSTFGVSIGNRTAKGKEEFRKYHASAIDVPGPEVDRLAAMAGKYKVYLV 127

Query: 122 MGVIERDGYTLYCTVLFFDPQGHYLGKHRKVMPTALERIIWGFGDGSTIPVFETTIGKIG 181
           MGVIERDGYTLYCTVLFFD QGH+LGKHRK+MPTALERIIWGFGDGSTIPV++T +GKIG
Sbjct: 128 MGVIERDGYTLYCTVLFFDSQGHFLGKHRKIMPTALERIIWGFGDGSTIPVYDTPLGKIG 187

Query: 182 AAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRK 241
           AAICWENRMPLLRTAMYAKGIEIYCAPTADSRD WQASMTHIALEGGCFVLSANQFCRRK
Sbjct: 188 AAICWENRMPLLRTAMYAKGIEIYCAPTADSRDVWQASMTHIALEGGCFVLSANQFCRRK 247

Query: 242 DYPPPPEYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLDLGDIAR 301
           DYPPPPEYVFSGTEE+L+PDS+VCAGGSVIISPSG +LAGPNY GEALISADLDLG+IAR
Sbjct: 248 DYPPPPEYVFSGTEEDLTPDSIVCAGGSVIISPSGAVLAGPNYVGEALISADLDLGEIAR 307

Query: 302 AKFDFDVVGHYARPEVLSLVVRDHPTPPATFTSTSSKTE 341
           AKFDFDVVGHYARPEVLSL+VRDH   P +FTSTSSK E
Sbjct: 308 AKFDFDVVGHYARPEVLSLIVRDHAVSPVSFTSTSSKAE 345

[Close]

BLAST of Sed0027695 vs. ExPASy Swiss-Prot
Match: Q42966 (Bifunctional nitrilase/nitrile hydratase NIT4B OS=Nicotiana tabacum OX=4097 GN=NIT4B PE=2 SV=1)

HSP 1 Score: 615.5 bits (1586), Expect = 3.6e-175
Identity = 297/337 (88.13%), Postives = 318/337 (94.36%), Query Frame = 0


Query: 4   NDLQLIPEVDMGSDSSVPTTVRATVVQASTIFYDTPATLDKAEKLLAEAAGFGSQLVVFP 63
           N+  +  EVDMG +SS P TVRATVVQASTIFYDTPATLDKAE+LLAEAA +G+QLVVFP
Sbjct: 10  NEGPMFAEVDMGDNSSTP-TVRATVVQASTIFYDTPATLDKAERLLAEAASYGAQLVVFP 69

Query: 64  EAFVGGYPRGSNFGVSIGNRTAKGKEEFRKYHAAAIDVPGPEVDRLAAMAGKYKVHLVMG 123
           EAF+GGYPRGS FGVSIGNRTAKGKEEFRKYHA+AIDVPGPEVDRLAAMAGKYKV+LVMG
Sbjct: 70  EAFIGGYPRGSTFGVSIGNRTAKGKEEFRKYHASAIDVPGPEVDRLAAMAGKYKVYLVMG 129

Query: 124 VIERDGYTLYCTVLFFDPQGHYLGKHRKVMPTALERIIWGFGDGSTIPVFETTIGKIGAA 183
           VIERDGYTLYCTVLFFD QGHYLGKHRK+MPTALERIIWGFGDGSTIPV++T +GKIGAA
Sbjct: 130 VIERDGYTLYCTVLFFDSQGHYLGKHRKIMPTALERIIWGFGDGSTIPVYDTPLGKIGAA 189

Query: 184 ICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRKDY 243
           ICWENRMPLLRTAMYAKGIEIYCAPTADSRD WQASMTHIALEGGCFVLSANQFCRRKDY
Sbjct: 190 ICWENRMPLLRTAMYAKGIEIYCAPTADSRDVWQASMTHIALEGGCFVLSANQFCRRKDY 249

Query: 244 PPPPEYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLDLGDIARAK 303
           PPPPEYVFSGT E+L+PDS+VCAGGSVIISPSG +LAGPNY+GEALISADLDLG+IARAK
Sbjct: 250 PPPPEYVFSGT-EDLTPDSIVCAGGSVIISPSGAVLAGPNYEGEALISADLDLGEIARAK 309

Query: 304 FDFDVVGHYARPEVLSLVVRDHPTPPATFTSTSSKTE 341
           FDFDVVGHYARPEVLSL+VRDH   P +FTSTSSK E
Sbjct: 310 FDFDVVGHYARPEVLSLIVRDHAVSPVSFTSTSSKAE 344

[Close]

BLAST of Sed0027695 vs. ExPASy Swiss-Prot
Match: Q5QGZ8 (Bifunctional nitrilase/nitrile hydratase NIT4A OS=Lupinus angustifolius OX=3871 GN=NIT4A PE=1 SV=1)

HSP 1 Score: 608.6 bits (1568), Expect = 4.4e-173
Identity = 292/337 (86.65%), Postives = 316/337 (93.77%), Query Frame = 0


Query: 4   NDLQLIPEVDMGSDSSVPTTVRATVVQASTIFYDTPATLDKAEKLLAEAAGFGSQLVVFP 63
           ND  L  EV+M SD + P TVRATVVQASTIFYDTPATLDKAE+LLAEAA +G+Q+VVFP
Sbjct: 10  NDGPLFAEVNMSSDFNAP-TVRATVVQASTIFYDTPATLDKAERLLAEAASYGAQIVVFP 69

Query: 64  EAFVGGYPRGSNFGVSIGNRTAKGKEEFRKYHAAAIDVPGPEVDRLAAMAGKYKVHLVMG 123
           EAF+GGYPRGSNFGVSIGNRTAKGKE+FRKYH+AAIDVPGPEVDRLAA+AGKYKV+LVMG
Sbjct: 70  EAFIGGYPRGSNFGVSIGNRTAKGKEDFRKYHSAAIDVPGPEVDRLAALAGKYKVYLVMG 129

Query: 124 VIERDGYTLYCTVLFFDPQGHYLGKHRKVMPTALERIIWGFGDGSTIPVFETTIGKIGAA 183
           VIERDGYTLYCTVLFF  QG YLGKHRK+MPTALERIIWGFGDGSTIPVFET IGKIGAA
Sbjct: 130 VIERDGYTLYCTVLFFGAQGRYLGKHRKLMPTALERIIWGFGDGSTIPVFETPIGKIGAA 189

Query: 184 ICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRKDY 243
           ICWEN+MPLLRTAMYAKG+EIYCAPTADSR+ WQASMTHIALEGGCFVLSANQFCRR+DY
Sbjct: 190 ICWENKMPLLRTAMYAKGVEIYCAPTADSREVWQASMTHIALEGGCFVLSANQFCRRRDY 249

Query: 244 PPPPEYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLDLGDIARAK 303
           PPPPEYVF GTEE L+PDSVVCAGGSVIISPSG +LAGP+Y+GEALISADLDLG+IARAK
Sbjct: 250 PPPPEYVFEGTEENLTPDSVVCAGGSVIISPSGAVLAGPSYEGEALISADLDLGEIARAK 309

Query: 304 FDFDVVGHYARPEVLSLVVRDHPTPPATFTSTSSKTE 341
           FDFDVVGHY+RPEVLSLVV+DHPT P TFTS S+K E
Sbjct: 310 FDFDVVGHYSRPEVLSLVVKDHPTNPVTFTSASTKIE 345

[Close]

BLAST of Sed0027695 vs. ExPASy Swiss-Prot
Match: Q3LRV4 (Bifunctional nitrilase/nitrile hydratase NIT4B OS=Lupinus angustifolius OX=3871 GN=NIT4B PE=1 SV=1)

HSP 1 Score: 606.3 bits (1562), Expect = 2.2e-172
Identity = 293/337 (86.94%), Postives = 314/337 (93.18%), Query Frame = 0


Query: 4   NDLQLIPEVDMGSDSSVPTTVRATVVQASTIFYDTPATLDKAEKLLAEAAGFGSQLVVFP 63
           ND  L  EVDM S     TTVRATVVQASTIFYDTPATLDKAE+LL +AA +G+Q+VVFP
Sbjct: 11  NDEPLFAEVDMAS-YFTSTTVRATVVQASTIFYDTPATLDKAERLLVQAASYGAQIVVFP 70

Query: 64  EAFVGGYPRGSNFGVSIGNRTAKGKEEFRKYHAAAIDVPGPEVDRLAAMAGKYKVHLVMG 123
           EAF+GGYPRGSNFGVSIGNRTAKGKEEFRKYH+AAIDVPGPEVDRL+AMAGKYKV+LVMG
Sbjct: 71  EAFIGGYPRGSNFGVSIGNRTAKGKEEFRKYHSAAIDVPGPEVDRLSAMAGKYKVYLVMG 130

Query: 124 VIERDGYTLYCTVLFFDPQGHYLGKHRKVMPTALERIIWGFGDGSTIPVFETTIGKIGAA 183
           VIERDGYTLYCTVLFFD QG YLGKHRKVMPTALERIIWGFGDGSTIPVF+T IGKIGAA
Sbjct: 131 VIERDGYTLYCTVLFFDSQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFQTPIGKIGAA 190

Query: 184 ICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRKDY 243
           ICWEN+MPLLRTAMYAKG+EIYCAPTADSRD WQAS THIALEGGCFVLSANQFCRRKDY
Sbjct: 191 ICWENKMPLLRTAMYAKGVEIYCAPTADSRDLWQASTTHIALEGGCFVLSANQFCRRKDY 250

Query: 244 PPPPEYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLDLGDIARAK 303
           PPPPEYVFSGTEE+L+PDSVV AGGSVIISPSG +LAGPNY+GEALISADLDLG+IARAK
Sbjct: 251 PPPPEYVFSGTEEDLTPDSVVSAGGSVIISPSGAVLAGPNYEGEALISADLDLGEIARAK 310

Query: 304 FDFDVVGHYARPEVLSLVVRDHPTPPATFTSTSSKTE 341
           FDFDVVGHY+R EVLSL+V+DHPT P TFTSTS+K E
Sbjct: 311 FDFDVVGHYSRSEVLSLIVKDHPTNPVTFTSTSTKIE 346

[Close]

BLAST of Sed0027695 vs. ExPASy Swiss-Prot
Match: Q6H849 (Bifunctional nitrilase/nitrile hydratase NIT4 OS=Oryza sativa subsp. japonica OX=39947 GN=NIT4 PE=2 SV=1)

HSP 1 Score: 562.8 bits (1449), Expect = 2.7e-159
Identity = 275/337 (81.60%), Postives = 298/337 (88.43%), Query Frame = 0


Query: 8   LIPEVDM-GSDSSVPTTVRATVVQASTIFYDTPATLDKAEKLLAEAAGFGSQLVVFPEAF 67
           +I EV+M G   S   TVRATVVQAST+FYDTPATLDKAE+L+ EAAG+GSQLVVFPEAF
Sbjct: 14  VIAEVEMNGGADSGAATVRATVVQASTVFYDTPATLDKAERLIEEAAGYGSQLVVFPEAF 73

Query: 68  VGGYPRGSNFG----VSIGNRTAKGKEEFRKYHAAAIDVPGPEVDRLAAMAGKYKVHLVM 127
           VGGYPRGS FG    +SIGN   KGKEEFRKYHAAAI+VPGPEV RLAAMAGKYKV LVM
Sbjct: 74  VGGYPRGSTFGFGANISIGNPKDKGKEEFRKYHAAAIEVPGPEVTRLAAMAGKYKVFLVM 133

Query: 128 GVIERDGYTLYCTVLFFDPQGHYLGKHRKVMPTALERIIWGFGDGSTIPVFETTIGKIGA 187
           GVIER+GYTLYC+VLFFDP G YLGKHRK+MPTALERIIWGFGDGSTIPV++T +GKIGA
Sbjct: 134 GVIEREGYTLYCSVLFFDPLGRYLGKHRKLMPTALERIIWGFGDGSTIPVYDTPLGKIGA 193

Query: 188 AICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRKD 247
            ICWEN+MPLLRTA+Y KGIEIYCAPTADSR  WQASMTHIALEGGCFVLSANQFCRRKD
Sbjct: 194 LICWENKMPLLRTALYGKGIEIYCAPTADSRQVWQASMTHIALEGGCFVLSANQFCRRKD 253

Query: 248 YPPPPEYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLDLGDIARA 307
           YPPPPEYVFSG  EE SPD+VVC GGSVIISPSG +LAGPNY+GEALI+ADLDLG+I RA
Sbjct: 254 YPPPPEYVFSGLGEEPSPDTVVCPGGSVIISPSGEVLAGPNYEGEALITADLDLGEIVRA 313

Query: 308 KFDFDVVGHYARPEVLSLVVRDHPTPPATFTSTSSKT 340
           KFDFDVVGHYARPEVLSLVV D P  P +FTS + KT
Sbjct: 314 KFDFDVVGHYARPEVLSLVVNDQPHLPVSFTSAAEKT 350

[Close]

BLAST of Sed0027695 vs. ExPASy TrEMBL
Match: A0A6J1EHI6 (bifunctional nitrilase/nitrile hydratase NIT4A OS=Cucurbita moschata OX=3662 GN=LOC111434356 PE=3 SV=1)

HSP 1 Score: 664.5 bits (1713), Expect = 2.5e-187
Identity = 326/344 (94.77%), Postives = 332/344 (96.51%), Query Frame = 0


Query: 2   AANDLQLIPEVDMGSDSSVPTTVRATVVQASTIFYDTPATLDKAEKLLAEAAGFGSQLVV 61
           AA D QLI EVDMGSD SVP TVRATVVQASTIFYDTPATLDKAE+LLAEAAGFGSQLVV
Sbjct: 8   AAADTQLIAEVDMGSDVSVP-TVRATVVQASTIFYDTPATLDKAERLLAEAAGFGSQLVV 67

Query: 62  FPEAFVGGYPRGSNFGVSIGNRTAKGKEEFRKYHAAAIDVPGPEVDRLAAMAGKYKVHLV 121
           FPEAFVGGYPRGSNFGVSIGNRT KGKEEFRKYHAAAIDVPGPEVDRLAAMAGKY+VHLV
Sbjct: 68  FPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGKYRVHLV 127

Query: 122 MGVIERDGYTLYCTVLFFDPQGHYLGKHRKVMPTALERIIWGFGDGSTIPVFETTIGKIG 181
           MGVIERDGYTLYCTVLFFDPQG YLGKHRKVMPTALERIIWGFGDGSTIPVFET+IGKIG
Sbjct: 128 MGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFETSIGKIG 187

Query: 182 AAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRK 241
           AAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRK
Sbjct: 188 AAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRK 247

Query: 242 DYPPPPEYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLDLGDIAR 301
           DYPPPP+YVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLDLGDIAR
Sbjct: 248 DYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLDLGDIAR 307

Query: 302 AKFDFDVVGHYARPEVLSLVVRDHPTPPATFTSTSSKTEGFCKN 346
           AKFDFDVVGHYARPEVLSLVVRDHPT P TFTS ++KTE  CKN
Sbjct: 308 AKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSATAKTEDSCKN 350

[Close]

BLAST of Sed0027695 vs. ExPASy TrEMBL
Match: A0A6J1I7W0 (bifunctional nitrilase/nitrile hydratase NIT4A OS=Cucurbita maxima OX=3661 GN=LOC111471660 PE=3 SV=1)

HSP 1 Score: 664.1 bits (1712), Expect = 3.2e-187
Identity = 326/342 (95.32%), Postives = 330/342 (96.49%), Query Frame = 0


Query: 2   AANDLQLIPEVDMGSDSSVPTTVRATVVQASTIFYDTPATLDKAEKLLAEAAGFGSQLVV 61
           AAND QLI EVDMGSD SVP TVRATVVQASTIFYDTPATLDKAE+LLAEAAGFGSQLVV
Sbjct: 8   AANDTQLIAEVDMGSDVSVP-TVRATVVQASTIFYDTPATLDKAERLLAEAAGFGSQLVV 67

Query: 62  FPEAFVGGYPRGSNFGVSIGNRTAKGKEEFRKYHAAAIDVPGPEVDRLAAMAGKYKVHLV 121
           FPEAFVGGYPRGSNFGVSIGNRT KGKEEFRKYHAAAIDVPGPEVDRLAAMAGKY+VHLV
Sbjct: 68  FPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGKYRVHLV 127

Query: 122 MGVIERDGYTLYCTVLFFDPQGHYLGKHRKVMPTALERIIWGFGDGSTIPVFETTIGKIG 181
           MGVIERDGYTLYCTVLFFDPQG YLGKHRKVMPTALERIIWGFGDGSTIPVFET+IGKIG
Sbjct: 128 MGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFETSIGKIG 187

Query: 182 AAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRK 241
           AAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRK
Sbjct: 188 AAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRK 247

Query: 242 DYPPPPEYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLDLGDIAR 301
           DYPPPP+YVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLDLGDIAR
Sbjct: 248 DYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLDLGDIAR 307

Query: 302 AKFDFDVVGHYARPEVLSLVVRDHPTPPATFTSTSSKTEGFC 344
           AKFDFDVVGHYARPEVLSLVVRDHPT P TFTS S KTE  C
Sbjct: 308 AKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSASKKTEDSC 348

[Close]

BLAST of Sed0027695 vs. ExPASy TrEMBL
Match: A0A0A0LVI0 (CN hydrolase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G168360 PE=3 SV=1)

HSP 1 Score: 654.8 bits (1688), Expect = 2.0e-184
Identity = 321/343 (93.59%), Postives = 328/343 (95.63%), Query Frame = 0


Query: 2   AANDLQLIPEVDMGSDSSVPTTVRATVVQASTIFYDTPATLDKAEKLLAEAAGFGSQLVV 61
           A  D QLI EVDMGSDSSV   VRATVVQASTIFYDTPATLDKAE+LLAEAAGFGSQLVV
Sbjct: 8   APTDSQLIAEVDMGSDSSV-MNVRATVVQASTIFYDTPATLDKAERLLAEAAGFGSQLVV 67

Query: 62  FPEAFVGGYPRGSNFGVSIGNRTAKGKEEFRKYHAAAIDVPGPEVDRLAAMAGKYKVHLV 121
           FPEAFVGGYPRGSNFGVSIGNRT KGKEEFRKYHA+AIDVPGPEVDRLAAMAGKY+VHLV
Sbjct: 68  FPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHASAIDVPGPEVDRLAAMAGKYRVHLV 127

Query: 122 MGVIERDGYTLYCTVLFFDPQGHYLGKHRKVMPTALERIIWGFGDGSTIPVFETTIGKIG 181
           MGVIERDGYTLYCTVLFFDPQG YLGKHRKVMPTALERIIWGFGDGSTIPVFET+IGKIG
Sbjct: 128 MGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFETSIGKIG 187

Query: 182 AAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRK 241
           AAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRK
Sbjct: 188 AAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRK 247

Query: 242 DYPPPPEYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLDLGDIAR 301
           DYPPPPEYVFSGTEEEL+PDSVVCAGGS IISPSGTILAGPNYDGEALISADLDLG+IAR
Sbjct: 248 DYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLDLGEIAR 307

Query: 302 AKFDFDVVGHYARPEVLSLVVRDHPTPPATFTSTSSKTEGFCK 345
           AKFDFDVVGHYARPEVLSLVVRDHPT P TFTSTS+K E  CK
Sbjct: 308 AKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKVEDSCK 349

[Close]

BLAST of Sed0027695 vs. ExPASy TrEMBL
Match: A0A5A7SY83 (Bifunctional nitrilase/nitrile hydratase NIT4A OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G002030 PE=3 SV=1)

HSP 1 Score: 653.7 bits (1685), Expect = 4.4e-184
Identity = 321/343 (93.59%), Postives = 328/343 (95.63%), Query Frame = 0


Query: 2   AANDLQLIPEVDMGSDSSVPTTVRATVVQASTIFYDTPATLDKAEKLLAEAAGFGSQLVV 61
           A  D QLI EVDMG+DSSV  TVRATVVQASTIFYDTPATLDKAE+LLAEAAGFGSQLVV
Sbjct: 8   APTDPQLIAEVDMGTDSSV-MTVRATVVQASTIFYDTPATLDKAERLLAEAAGFGSQLVV 67

Query: 62  FPEAFVGGYPRGSNFGVSIGNRTAKGKEEFRKYHAAAIDVPGPEVDRLAAMAGKYKVHLV 121
           FPEAFVGGYPRGSNFGVSIGNRT KGKEEFRKYHAAAIDVPGPEVDRLAAMAGKY+VHLV
Sbjct: 68  FPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGKYRVHLV 127

Query: 122 MGVIERDGYTLYCTVLFFDPQGHYLGKHRKVMPTALERIIWGFGDGSTIPVFETTIGKIG 181
           MGVIERDGYTLYCTVLFFDPQG YLGKHRKVMPTALERIIWGFGDGSTIPVFET+IGKIG
Sbjct: 128 MGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFETSIGKIG 187

Query: 182 AAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRK 241
           AAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRK
Sbjct: 188 AAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRK 247

Query: 242 DYPPPPEYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLDLGDIAR 301
           DYPPPPEYVFSGTEEEL+PDSVVCAGGS IISPSGTILAGPNYDGEALISADLDLG+IAR
Sbjct: 248 DYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLDLGEIAR 307

Query: 302 AKFDFDVVGHYARPEVLSLVVRDHPTPPATFTSTSSKTEGFCK 345
           AKFDFDVVGHYARPEVLSLVVRDHP  P TFTSTS+K E  CK
Sbjct: 308 AKFDFDVVGHYARPEVLSLVVRDHPAIPVTFTSTSTKAEDSCK 349

[Close]

BLAST of Sed0027695 vs. ExPASy TrEMBL
Match: A0A1S3CLB4 (bifunctional nitrilase/nitrile hydratase NIT4A OS=Cucumis melo OX=3656 GN=LOC103501743 PE=3 SV=1)

HSP 1 Score: 653.7 bits (1685), Expect = 4.4e-184
Identity = 321/343 (93.59%), Postives = 328/343 (95.63%), Query Frame = 0


Query: 2   AANDLQLIPEVDMGSDSSVPTTVRATVVQASTIFYDTPATLDKAEKLLAEAAGFGSQLVV 61
           A  D QLI EVDMG+DSSV  TVRATVVQASTIFYDTPATLDKAE+LLAEAAGFGSQLVV
Sbjct: 8   APTDPQLIAEVDMGTDSSV-MTVRATVVQASTIFYDTPATLDKAERLLAEAAGFGSQLVV 67

Query: 62  FPEAFVGGYPRGSNFGVSIGNRTAKGKEEFRKYHAAAIDVPGPEVDRLAAMAGKYKVHLV 121
           FPEAFVGGYPRGSNFGVSIGNRT KGKEEFRKYHAAAIDVPGPEVDRLAAMAGKY+VHLV
Sbjct: 68  FPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGKYRVHLV 127

Query: 122 MGVIERDGYTLYCTVLFFDPQGHYLGKHRKVMPTALERIIWGFGDGSTIPVFETTIGKIG 181
           MGVIERDGYTLYCTVLFFDPQG YLGKHRKVMPTALERIIWGFGDGSTIPVFET+IGKIG
Sbjct: 128 MGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFETSIGKIG 187

Query: 182 AAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRK 241
           AAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRK
Sbjct: 188 AAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRK 247

Query: 242 DYPPPPEYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLDLGDIAR 301
           DYPPPPEYVFSGTEEEL+PDSVVCAGGS IISPSGTILAGPNYDGEALISADLDLG+IAR
Sbjct: 248 DYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLDLGEIAR 307

Query: 302 AKFDFDVVGHYARPEVLSLVVRDHPTPPATFTSTSSKTEGFCK 345
           AKFDFDVVGHYARPEVLSLVVRDHP  P TFTSTS+K E  CK
Sbjct: 308 AKFDFDVVGHYARPEVLSLVVRDHPAIPVTFTSTSTKAEDSCK 349

[Close]

BLAST of Sed0027695 vs. TAIR 10
Match: AT5G22300.1 (nitrilase 4 )

HSP 1 Score: 552.4 bits (1422), Expect = 2.6e-157
Identity = 265/332 (79.82%), Postives = 298/332 (89.76%), Query Frame = 0


Query: 4   NDLQLIPEVDMGSDSSVPTTVRATVVQASTIFYDTPATLDKAEKLLAEAAGFGSQLVVFP 63
           N  Q+ PE+DM +  S  + VRATVVQAST+FYDTPATLDKAE+LL+EAA  GSQLVVFP
Sbjct: 17  NGHQIFPEIDMSAGDS-SSIVRATVVQASTVFYDTPATLDKAERLLSEAAENGSQLVVFP 76

Query: 64  EAFVGGYPRGSNFGVSIGNRTAKGKEEFRKYHAAAIDVPGPEVDRLAAMAGKYKVHLVMG 123
           EAF+GGYPRGS F ++IG+RTAKG+++FRKYHA+AIDVPGPEV+RLA MA KYKV+LVMG
Sbjct: 77  EAFIGGYPRGSTFELAIGSRTAKGRDDFRKYHASAIDVPGPEVERLALMAKKYKVYLVMG 136

Query: 124 VIERDGYTLYCTVLFFDPQGHYLGKHRKVMPTALERIIWGFGDGSTIPVFETTIGKIGAA 183
           VIER+GYTLYCTVLFFD QG +LGKHRK+MPTALER IWGFGDGSTIPVF+T IGKIGAA
Sbjct: 137 VIEREGYTLYCTVLFFDSQGLFLGKHRKLMPTALERCIWGFGDGSTIPVFDTPIGKIGAA 196

Query: 184 ICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRKDY 243
           ICWENRMP LRTAMYAKGIEIYCAPTADSR+TW ASMTHIALEGGCFVLSANQFCRRKDY
Sbjct: 197 ICWENRMPSLRTAMYAKGIEIYCAPTADSRETWLASMTHIALEGGCFVLSANQFCRRKDY 256

Query: 244 PPPPEYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLDLGDIARAK 303
           P PPEY+FSG+EE L+PDSVVCAGGS IISP G +LAGPNY GEALI+ADLDLGDIARAK
Sbjct: 257 PSPPEYMFSGSEESLTPDSVVCAGGSSIISPLGIVLAGPNYRGEALITADLDLGDIARAK 316

Query: 304 FDFDVVGHYARPEVLSLVVRDHPTPPATFTST 336
           FDFDVVGHY+RPEV SL +R+HP    +F ++
Sbjct: 317 FDFDVVGHYSRPEVFSLNIREHPRKAVSFKTS 347

[Close]

BLAST of Sed0027695 vs. TAIR 10
Match: AT3G44300.1 (nitrilase 2 )

HSP 1 Score: 467.6 bits (1202), Expect = 8.5e-132
Identity = 216/319 (67.71%), Postives = 259/319 (81.19%), Query Frame = 0


Query: 22  TTVRATVVQASTIFYDTPATLDKAEKLLAEAAGFGSQLVVFPEAFVGGYPRGSNFGVSIG 81
           T VRAT+VQAST++ DTPATL+KA K + EAA  GS+LVVFPEAF+GGYPRG  FG+ +G
Sbjct: 16  TIVRATIVQASTVYNDTPATLEKANKFIVEAASKGSELVVFPEAFIGGYPRGFRFGLGVG 75

Query: 82  NRTAKGKEEFRKYHAAAIDVPGPEVDRLAAMAGKYKVHLVMGVIERDGYTLYCTVLFFDP 141
               +G++EFRKYHA+AI VPGPEV++LA +AGK  V+LVMG IE+DGYTLYCT LFF P
Sbjct: 76  VHNEEGRDEFRKYHASAIKVPGPEVEKLAELAGKNNVYLVMGAIEKDGYTLYCTALFFSP 135

Query: 142 QGHYLGKHRKVMPTALERIIWGFGDGSTIPVFETTIGKIGAAICWENRMPLLRTAMYAKG 201
           QG +LGKHRK+MPT+LER IWG GDGSTIPV++T IGK+GAAICWENRMPL RTA+YAKG
Sbjct: 136 QGQFLGKHRKLMPTSLERCIWGQGDGSTIPVYDTPIGKLGAAICWENRMPLYRTALYAKG 195

Query: 202 IEIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRKDYPPPPEYVFSGTEEELSPD 261
           IE+YCAPTAD    WQ+SM HIA+EGGCFVLSA QFC RKD+P  P+Y+F+   ++  PD
Sbjct: 196 IELYCAPTADGSKEWQSSMLHIAIEGGCFVLSACQFCLRKDFPDHPDYLFTDWYDDKEPD 255

Query: 262 SVVCAGGSVIISPSGTILAGPNYDGEALISADLDLGDIARAKFDFDVVGHYARPEVLSLV 321
           S+V  GGSVIISP G +LAGPN++ E LI+ADLDLGD+ARAK  FD VGHY+RP+VL L 
Sbjct: 256 SIVSQGGSVIISPLGQVLAGPNFESEGLITADLDLGDVARAKLYFDSVGHYSRPDVLHLT 315

Query: 322 VRDHPTPPATFTSTSSKTE 341
           V +HP  P TF S   K E
Sbjct: 316 VNEHPKKPVTFISKVEKAE 334

[Close]

BLAST of Sed0027695 vs. TAIR 10
Match: AT3G44320.1 (nitrilase 3 )

HSP 1 Score: 464.2 bits (1193), Expect = 9.4e-131
Identity = 216/327 (66.06%), Postives = 263/327 (80.43%), Query Frame = 0


Query: 14  MGSDSSVPTTVRATVVQASTIFYDTPATLDKAEKLLAEAAGFGSQLVVFPEAFVGGYPRG 73
           +G D S  +TVR T+VQ+ST++ DTPATLDKAEK + EAA  G++LV+FPEAF+GGYPRG
Sbjct: 17  IGVDPS--STVRVTIVQSSTVYNDTPATLDKAEKFIVEAASKGAKLVLFPEAFIGGYPRG 76

Query: 74  SNFGVSIGNRTAKGKEEFRKYHAAAIDVPGPEVDRLAAMAGKYKVHLVMGVIERDGYTLY 133
             FG+++G    +G++EFR YHA+AI VPGPEV+RLA +AGK  VHLVMG IE+DGYTLY
Sbjct: 77  FRFGLAVGVHNEEGRDEFRNYHASAIKVPGPEVERLAELAGKNNVHLVMGAIEKDGYTLY 136

Query: 134 CTVLFFDPQGHYLGKHRKVMPTALERIIWGFGDGSTIPVFETTIGKIGAAICWENRMPLL 193
           CT LFF PQG +LGKHRKVMPT+LER IWG GDGSTIPV++T IGKIGAAICWENRMPL 
Sbjct: 137 CTALFFSPQGQFLGKHRKVMPTSLERCIWGQGDGSTIPVYDTPIGKIGAAICWENRMPLY 196

Query: 194 RTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRKDYPPPPEYVFSG 253
           RTA+YAKGIEIYCAPTAD    WQASM HIA+EGGCFVLSA+QFC+R+++P  P+Y+F+ 
Sbjct: 197 RTALYAKGIEIYCAPTADYSLEWQASMIHIAVEGGCFVLSAHQFCKRREFPEHPDYLFND 256

Query: 254 TEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLDLGDIARAKFDFDVVGHYA 313
             +    D  V  GGSVIISP G +LAGPNY+ E L++ADLDLGDIARAK  FDVVGHY+
Sbjct: 257 IVDTKEHDPTVSGGGSVIISPLGKVLAGPNYESEGLVTADLDLGDIARAKLYFDVVGHYS 316

Query: 314 RPEVLSLVVRDHPTPPATFTSTSSKTE 341
           +P++ +L V +HP  P TF +   K E
Sbjct: 317 KPDIFNLTVNEHPKKPVTFMTKVEKAE 341

[Close]

BLAST of Sed0027695 vs. TAIR 10
Match: AT3G44310.1 (nitrilase 1 )

HSP 1 Score: 451.8 bits (1161), Expect = 4.9e-127
Identity = 208/319 (65.20%), Postives = 257/319 (80.56%), Query Frame = 0


Query: 22  TTVRATVVQASTIFYDTPATLDKAEKLLAEAAGFGSQLVVFPEAFVGGYPRGSNFGVSIG 81
           TTVR T+VQ+ST++ DTPAT+DKAEK + EAA  G++LV+FPE F+GGYPRG  FG+++G
Sbjct: 23  TTVRVTIVQSSTVYNDTPATIDKAEKYIVEAASKGAELVLFPEGFIGGYPRGFRFGLAVG 82

Query: 82  NRTAKGKEEFRKYHAAAIDVPGPEVDRLAAMAGKYKVHLVMGVIERDGYTLYCTVLFFDP 141
               +G++EFRKYHA+AI VPGPEV RLA +A K  V+LVMG IE++GYTLYCTVLFF P
Sbjct: 83  VHNEEGRDEFRKYHASAIHVPGPEVARLADVARKNHVYLVMGAIEKEGYTLYCTVLFFSP 142

Query: 142 QGHYLGKHRKVMPTALERIIWGFGDGSTIPVFETTIGKIGAAICWENRMPLLRTAMYAKG 201
           QG +LGKHRK+MPT+LER IWG GDGSTIPV++T IGK+GAAICWENRMPL RTA+YAKG
Sbjct: 143 QGQFLGKHRKLMPTSLERCIWGQGDGSTIPVYDTPIGKLGAAICWENRMPLYRTALYAKG 202

Query: 202 IEIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRKDYPPPPEYVFSGTEEELSPD 261
           IE+YCAPTAD    WQ+SM HIA+EGGCFVLSA QFC+RK +P  P+Y+F+   ++   D
Sbjct: 203 IELYCAPTADGSKEWQSSMLHIAIEGGCFVLSACQFCQRKHFPDHPDYLFTDWYDDKEHD 262

Query: 262 SVVCAGGSVIISPSGTILAGPNYDGEALISADLDLGDIARAKFDFDVVGHYARPEVLSLV 321
           S+V  GGSVIISP G +LAGPN++ E L++AD+DLGDIARAK  FD VGHY+RP+VL L 
Sbjct: 263 SIVSQGGSVIISPLGQVLAGPNFESEGLVTADIDLGDIARAKLYFDSVGHYSRPDVLHLT 322

Query: 322 VRDHPTPPATFTSTSSKTE 341
           V +HP    TF +   K E
Sbjct: 323 VNEHPRKSVTFVTKVEKAE 341

[Close]

BLAST of Sed0027695 vs. TAIR 10
Match: AT3G44310.3 (nitrilase 1 )

HSP 1 Score: 451.8 bits (1161), Expect = 4.9e-127
Identity = 208/319 (65.20%), Postives = 257/319 (80.56%), Query Frame = 0


Query: 22  TTVRATVVQASTIFYDTPATLDKAEKLLAEAAGFGSQLVVFPEAFVGGYPRGSNFGVSIG 81
           TTVR T+VQ+ST++ DTPAT+DKAEK + EAA  G++LV+FPE F+GGYPRG  FG+++G
Sbjct: 23  TTVRVTIVQSSTVYNDTPATIDKAEKYIVEAASKGAELVLFPEGFIGGYPRGFRFGLAVG 82

Query: 82  NRTAKGKEEFRKYHAAAIDVPGPEVDRLAAMAGKYKVHLVMGVIERDGYTLYCTVLFFDP 141
               +G++EFRKYHA+AI VPGPEV RLA +A K  V+LVMG IE++GYTLYCTVLFF P
Sbjct: 83  VHNEEGRDEFRKYHASAIHVPGPEVARLADVARKNHVYLVMGAIEKEGYTLYCTVLFFSP 142

Query: 142 QGHYLGKHRKVMPTALERIIWGFGDGSTIPVFETTIGKIGAAICWENRMPLLRTAMYAKG 201
           QG +LGKHRK+MPT+LER IWG GDGSTIPV++T IGK+GAAICWENRMPL RTA+YAKG
Sbjct: 143 QGQFLGKHRKLMPTSLERCIWGQGDGSTIPVYDTPIGKLGAAICWENRMPLYRTALYAKG 202

Query: 202 IEIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRKDYPPPPEYVFSGTEEELSPD 261
           IE+YCAPTAD    WQ+SM HIA+EGGCFVLSA QFC+RK +P  P+Y+F+   ++   D
Sbjct: 203 IELYCAPTADGSKEWQSSMLHIAIEGGCFVLSACQFCQRKHFPDHPDYLFTDWYDDKEHD 262

Query: 262 SVVCAGGSVIISPSGTILAGPNYDGEALISADLDLGDIARAKFDFDVVGHYARPEVLSLV 321
           S+V  GGSVIISP G +LAGPN++ E L++AD+DLGDIARAK  FD VGHY+RP+VL L 
Sbjct: 263 SIVSQGGSVIISPLGQVLAGPNFESEGLVTADIDLGDIARAKLYFDSVGHYSRPDVLHLT 322

Query: 322 VRDHPTPPATFTSTSSKTE 341
           V +HP    TF +   K E
Sbjct: 323 VNEHPRKSVTFVTKVEKAE 341

The following BLAST results are available for this feature:

BLAST of Sed0027695 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022927562.1	5.1e-187	94.77	bifunctional nitrilase/nitrile hydratase NIT4A [Cucurbita moschata]	[more]
XP_023520715.1	6.7e-187	95.04	bifunctional nitrilase/nitrile hydratase NIT4A [Cucurbita pepo subsp. pepo]	[more]
XP_022973146.1	6.7e-187	95.32	bifunctional nitrilase/nitrile hydratase NIT4A [Cucurbita maxima]	[more]
KAG6583706.1	3.3e-186	94.46	Bifunctional nitrilase/nitrile hydratase NIT4A, partial [Cucurbita argyrosperma ...	[more]
XP_004139500.1	4.0e-184	93.59	bifunctional nitrilase/nitrile hydratase NIT4A [Cucumis sativus] >KGN64959.1 hyp...	[more]

BLAST of Sed0027695 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q42965	9.4e-176	87.91	Bifunctional nitrilase/nitrile hydratase NIT4A OS=Nicotiana tabacum OX=4097 GN=N...	[more]
Q42966	3.6e-175	88.13	Bifunctional nitrilase/nitrile hydratase NIT4B OS=Nicotiana tabacum OX=4097 GN=N...	[more]
Q5QGZ8	4.4e-173	86.65	Bifunctional nitrilase/nitrile hydratase NIT4A OS=Lupinus angustifolius OX=3871 ...	[more]
Q3LRV4	2.2e-172	86.94	Bifunctional nitrilase/nitrile hydratase NIT4B OS=Lupinus angustifolius OX=3871 ...	[more]
Q6H849	2.7e-159	81.60	Bifunctional nitrilase/nitrile hydratase NIT4 OS=Oryza sativa subsp. japonica OX...	[more]

BLAST of Sed0027695 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EHI6	2.5e-187	94.77	bifunctional nitrilase/nitrile hydratase NIT4A OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A6J1I7W0	3.2e-187	95.32	bifunctional nitrilase/nitrile hydratase NIT4A OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A0A0LVI0	2.0e-184	93.59	CN hydrolase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G16836...	[more]
A0A5A7SY83	4.4e-184	93.59	Bifunctional nitrilase/nitrile hydratase NIT4A OS=Cucumis melo var. makuwa OX=11...	[more]
A0A1S3CLB4	4.4e-184	93.59	bifunctional nitrilase/nitrile hydratase NIT4A OS=Cucumis melo OX=3656 GN=LOC103...	[more]

BLAST of Sed0027695 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G22300.1	2.6e-157	79.82	nitrilase 4	[more]
AT3G44300.1	8.5e-132	67.71	nitrilase 2	[more]
AT3G44320.1	9.4e-131	66.06	nitrilase 3	[more]
AT3G44310.1	4.9e-127	65.20	nitrilase 1	[more]
AT3G44310.3	4.9e-127	65.20	nitrilase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003010	Carbon-nitrogen hydrolase	PFAM	PF00795	CN_hydrolase	coord: 25..303 e-value: 1.1E-69 score: 234.7
IPR003010	Carbon-nitrogen hydrolase	PROSITE	PS50263	CN_HYDROLASE	coord: 24..296 score: 58.292095
IPR036526	Carbon-nitrogen hydrolase superfamily	GENE3D	3.60.110.10		coord: 16..308 e-value: 4.2E-86 score: 290.4
IPR036526	Carbon-nitrogen hydrolase superfamily	SUPERFAMILY	56317	Carbon-nitrogen hydrolase	coord: 22..322
IPR044149	Nitrilase/Cyanide hydratase	PANTHER	PTHR46044	NITRILASE	coord: 7..339
IPR044149	Nitrilase/Cyanide hydratase	CDD	cd07564	nitrilases_CHs	coord: 24..324 e-value: 2.33785E-162 score: 453.483
None	No IPR available	PANTHER	PTHR46044:SF1	NITRILASE	coord: 7..339
IPR000132	Nitrilase/cyanide hydratase, conserved site	PROSITE	PS00921	NITRIL_CHT_2	coord: 181..194
IPR000132	Nitrilase/cyanide hydratase, conserved site	PROSITE	PS00920	NITRIL_CHT_1	coord: 56..71

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sed0027695.1	Sed0027695.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051410	detoxification of nitrogen compound
biological_process	GO:0006807	nitrogen compound metabolic process
molecular_function	GO:0047427	cyanoalanine nitrilase activity
molecular_function	GO:0018822	nitrile hydratase activity
molecular_function	GO:0003824	catalytic activity