Sed0027668 (gene) Chayote v1

Overview


Name	Sed0027668
Type	gene
Organism	Sechium edule (Chayote v1)
Description	tRNA pseudouridine synthase
Location	LG06: 39509595 .. 39514238 (+)
RNA-Seq Expression	Sed0027668
Synteny	Sed0027668

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTACTTTTAAGCAGAGCGTTGGGCTTCGACCAAATTGAAGCCGAAGCCCACAGTTGAAGATGTCGACAATCAAAACGACCTACCACGGCGCACCGGCAGTGACCATGGACGACTGAACTTCAACTTTCCTCAATCTCTTCGTCTTTAACAACCCTTTATACTTGTTCCAAAAACACCTGCTACATTGCTTAACTTAATAACCACATTGGATTCTGCGTCCGGTATCCCATAATCCTGGCGGCCGGCGGCGGCGAAAATCAATGACGGAAAACCAGAAGCATAGGCTGGAATTCAGTTCAGACTCATCTCCGGAAAGGCCGTTCAAGATTGGAAAGACGGCGGACGAAGCAGAACCCATGAACCCTAATCCTGCTGGAATCCAACGCTACCTTGTCGCAGTCGAATATGTTGGAACTCGCTTTTTCGGATCTCAGAAGCAACCCAATTGCCGTACGGTGGTTGGCGCGCTTGAGGTACATCTTTGCATTTTAACCAATCTAACACTTTCAAGACCTCTTGTTTTTTCTTTCTTTCATGATCGGCGTTTTCAAGCCCATCCGCTTTTCTCGCCTATGGCCATAAAAAAGCTAAGGCCAGCTAGGCCCTCGTCTTTCCTTGCCATTTTACGCCTTACATCGCCCTTGCCTCAGGCCCATTACCTCAGAAAGAAATCTAACAACCATCCAGACACTAAATTAGTTTAATTGTTATTTCAATATATATAGAACTGGCCCCAACGGGAATTGTAGTGATTGAAGACTTGGTCTGTGGGGGTATATGCTCCCCTCAAAGTCCCGAATTCGAGACTCACATGTGACATTACTCCTTTGATGTCTTCGGTGCCTGGCCTATGGACGGGTGTCGTTACGCTTGTTTCAAAAAATATATAGAACTATACTTTTGGTTAACTTTAATTTTTTTATTCAAATAATAATTTAATATGTATGGTCCCCAAGAGGTGACACAATCATCGAAGGCTTGAGCTTGGAAGGTATGCTCCCCTCAAGGTCCGACTCACGTGTGATTTTACTTCTTTGATGTCTTCCGATGCCTGGCCTAGGGATGGACGTGGTTACTCTTGTTTTAAAAAAATAATTTAAAATGTATGGTATATCTTATAATAGCATTTTATTTGTGTGATTTAGGTATGAATTTGAAATTTATGAGAATAGAAATATGTTTTATTTAACAATTTATGTTCAATATATTATTTAGTTATCCATTTATTTGTTGCGCCTCTTTTCACTCGAGTCTTGCATTTTTATCGCCTCTTACTTTGAGGCGATCAGGAAGAGTATCGCCTTGCGTTGTGCCTTGCGCTGTGAAAACACTTGTTCACGAAATGCTGTAGTTCATTTCATTCTAAATGATTACCTTACCATTGTCAGGAAGCGTTTCAGAAATTCATCGGCCAATCAGTTCCAGTGTTTTGTTCTAGCAGAACTGTAAGTGTATCTTCTTCTGGATATTGGTTACTTTTTACCATGCCTAAATAAATTTTATTGCTGTTATTATTTTGTTCTTACTCATTGTATAATTATAGGTATAGGATGTTATTAAACCAGATGAGCATTGAAATTTACTTAAGTTGAAAGGTATTTGATTGGAACTTATCTTGGTATTATTCAAATAGGGAGGTTTCACAACAAATGCCTAACTCTTACACCAATATAATTCTATGGACTCGTCCTAAGGGTTGGCATAGTGGTTGAAGACTTGGACTTTGAAGGTATGCTCCCTTCAAAGTTCCAGGTTCGAGACTCACCTGTAACATTACTCCTTTGATGTCTCCCGATACCTGGCCTAGGGACTGGCGTGGTTACCCTTGTTTAAAAAAAAAGCTCTTACACCAATATAGTTCTATCTTCTTGACTCACTCTACCACTTGGTATTTATAGACTTACTAAGTAGCGAACTCTTAACAAACTCTAGTTATGTAATCTAACCTAATTTGGGATCTATCAGTATTTTGAAGACCTATCTCTCTCAAGGTTCTACTAACTTTTATCTTGTTGATTCTTCGGTCCTCTGGTAGTTTTCCTATTGGCTTACCGATGGTATTGTTGATTAAAGATATATGGACATTGACTTACGTTACCTTTTAAGATCTCAATCGGATTGTGAAAAAATCGATTGAATTATTGCAAGATTAGTTGTTTTTCTGGCTTAGTTGCAGCAGAGCAGACTGCAATGCATCTTGCACCATACGAGTGTCGTGTTGTTCTTAGTAGAGATCCATGATTGAACTGTTATTTTAACTACATTGATTTCTTACCATTTTTCTGAAGTTCTTTATCTACTCCAAATTTATGGACCAACCTTATTCTTATTTCTTATAAATTATGCTTTAGAATAAATCTTTGGTTTCTGGCATTTTCTGCTGAATTACAACCAAACCAAGTATATGCTTTTGTAACTATATCGCTTAGTCGTTATTGTTATTTTATTGTTTTGCTTTCTCTGAGCTTTCTGTATCATTGCCATTCGTATTTCTATGCAGTGTTTTACATTTCTTGCTAACATAAGTATATTAGGCAATCCACGACACATGTTATAACTTTCCTTCTTTGTTCAATCAAGGATGCTGGAGTTCATGCCTTATCAAATGCTTTTCATGTCGATGTTGAGCGCATAAGCAAAAGAAAGCCCGGAGAAGTGGTTTGTCCCTCTATAATAGAATATCTATATCCATCTCCCCTCTCATATGTACAATTGTGATGCATGCTTAACTAGATGAGGAACTTTCTCTGAAAACTTAGTTCATTCATTGACTTTTTTTTTTGAAACAGTCCACTCATTGACTTTAATGCCGAGATTAAACTCTCTTATGCACAAAAACATATGAATTTTAAGAAATGCTGCTCGACTTTGCGCAAAGATGCTAACTTTTTTTCAACTTTCATGCAGTTACCTCCTCATGAACCAGGAGTCGTTCGTAGGGCTGTGAATCATTTCTTACAGGTTTGGTTTTCCTCAGACCAGTCACTTTATTGAGCCATATTTTTGAAGGCTATGAATGAGCCTTTGTTTTTTATTATATAACCAAAAAATGATGAATGCCTGAAAGGTATCTGTGTTGGTTTTGATTTCAGAAACAAGGTGATGTTATGGTAGTCGATGTTCGAAGTGTTCCCAATGATTTTCATGCTAGATATAAAGCTCAAGAGCGTACGTGAGTACCATTGATCTGTGCATCAGTAGTTTATACCGTTGTCACATATTAGATAGTTTAGTAGAATTAGCAATGAAGGCTATGCCTTATGATGCGATATTTAAGATAAGTAGTTTTGAATGTGATAGGCATTCTAATACTTTTTTGTATCACTTGAAATATTTTCAGCTACTTTTATCGGTTAGTTTCTGGGGAAGACGTATTGTCAAGCTTTGTGAAGGATCGGGCATGGCACGTGCCAGAGACATTGAATATTGGGGCCATGAAGGTATGAAAAAATTTACAATGTGTATTGTTTATTAGTTATTGCATTCTGGAACTGATGCAACATTACTGGTATGTATGAATTCAGGAGGCGTGCAAAGTGCTTGTTGGGCATCATGACTTTAGCTCCTTCAGGGCAGCCGGTTGTCAGGTCGCTTAAGACACATTAATCTTGTTCTCTCTGGTTTTTATTCGATAGGCAGGTTGTTAAACATAGAAGGCAGGTGAAGGAATGAAATGGTGTATGGTGTTGTATTGTAAATAATATGTTTAGATGTGCAGTGCAGGCAAACTCGCCAATCCGAACTCTGGATGAACTGGACATTTGCGAAGTAGCTTCAACGCCTTATTTTCCAACATTAACCGAGCGAAACCAATGGCAAGGTAAAGACGAGACTTGCGAGGTTGGATTTGGGATGAGGAGAAGGCATCAATGTTTTGTGGTGACCGCACGAGCACGCTCTTTTCTGTATCATCAGGTATAATATAGTATATATAACGTGTCCCTGAAGGGGTGGCATAGTGGTTAAAAACTTAGACTTTGAATTATTCTCCCCTTAAGGTCCAAGGTTCGATACTCACTTGTGACATTAATTTGTAGACATCTCCGGTGTCTCCATTCCTTTGATGTCTCGTGGTGTCTAGCCTAGGGATAGACATGATTATTCTGGGTATAGTAGAGTCTGAGATCGATTGGTCATTTCTCAGGTGAGACTGATGGTTGGAGTCCTTAAAGCGGTTGGCACAGGAAATCTAACAGTAGGAGATGGTGGGTAGTTTTTGTAATGATTAGAAAGAACATAGATAGATGTTTTTAAGGTAGGCTAATAATATTAATGTTTGATAATATGGTGTTTGTTGTTCCTTGCAGTAGAAAGAATCTTAGAAGCTAAGAGTGTGAATTCAACAAGTGCAATGGCCCCTGCATGCGGACTCTACCTCGGACATGTCAAATATGATCTACCAAACTGAAACCGACTTGTTCTCTCAACGTCAATCCAATTTTATTTATAACCCATTGCCAAAATCAATAGTTAGGTTAAATTTGAACCCCCCATTCATTTCTTCTGTATTAATTTCTTCGCCTATGCTAATTGGAAATTATTATAGTAACGATATTTTGTGATTTTCTTTCTTCAGCCTGGGGTATGTATACGGCTGAGAAATTTTGTTACAAATTAATCTTTCTAAAACAAGTTGTTACGGAT

mRNA sequence

CTACTTTTAAGCAGAGCGTTGGGCTTCGACCAAATTGAAGCCGAAGCCCACAGTTGAAGATGTCGACAATCAAAACGACCTACCACGGCGCACCGGCAGTGACCATGGACGACTGAACTTCAACTTTCCTCAATCTCTTCGTCTTTAACAACCCTTTATACTTGTTCCAAAAACACCTGCTACATTGCTTAACTTAATAACCACATTGGATTCTGCGTCCGGTATCCCATAATCCTGGCGGCCGGCGGCGGCGAAAATCAATGACGGAAAACCAGAAGCATAGGCTGGAATTCAGTTCAGACTCATCTCCGGAAAGGCCGTTCAAGATTGGAAAGACGGCGGACGAAGCAGAACCCATGAACCCTAATCCTGCTGGAATCCAACGCTACCTTGTCGCAGTCGAATATGTTGGAACTCGCTTTTTCGGATCTCAGAAGCAACCCAATTGCCGTACGGTGGTTGGCGCGCTTGAGGAAGCGTTTCAGAAATTCATCGGCCAATCAGTTCCAGTGTTTTGTTCTAGCAGAACTGATGCTGGAGTTCATGCCTTATCAAATGCTTTTCATGTCGATGTTGAGCGCATAAGCAAAAGAAAGCCCGGAGAAGTGTTACCTCCTCATGAACCAGGAGTCGTTCGTAGGGCTGTGAATCATTTCTTACAGAAACAAGGTGATGTTATGGTAGTCGATGTTCGAAGTGTTCCCAATGATTTTCATGCTAGATATAAAGCTCAAGAGCGTACCTACTTTTATCGGTTAGTTTCTGGGGAAGACGTATTGTCAAGCTTTGTGAAGGATCGGGCATGGCACGTGCCAGAGACATTGAATATTGGGGCCATGAAGGAGGCGTGCAAAGTGCTTGTTGGGCATCATGACTTTAGCTCCTTCAGGGCAGCCGGTTGTCAGGCAAACTCGCCAATCCGAACTCTGGATGAACTGGACATTTGCGAAGTAGCTTCAACGCCTTATTTTCCAACATTAACCGAGCGAAACCAATGGCAAGGTAAAGACGAGACTTGCGAGGTTGGATTTGGGATGAGGAGAAGGCATCAATGTTTTGTGGTGACCGCACGAGCACGCTCTTTTCTGTATCATCAGGTGAGACTGATGGTTGGAGTCCTTAAAGCGGTTGGCACAGGAAATCTAACAGTAGGAGATGTAGAAAGAATCTTAGAAGCTAAGAGTGTGAATTCAACAAGTGCAATGGCCCCTGCATGCGGACTCTACCTCGGACATGTCAAATATGATCTACCAAACTGAAACCGACTTGTTCTCTCAACGTCAATCCAATTTTATTTATAACCCATTGCCAAAATCAATAGTTAGGTTAAATTTGAACCCCCCATTCATTTCTTCTGTATTAATTTCTTCGCCTATGCTAATTGGAAATTATTATAGTAACGATATTTTGTGATTTTCTTTCTTCAGCCTGGGGTATGTATACGGCTGAGAAATTTTGTTACAAATTAATCTTTCTAAAACAAGTTGTTACGGAT

Coding sequence (CDS)

ATGACGGAAAACCAGAAGCATAGGCTGGAATTCAGTTCAGACTCATCTCCGGAAAGGCCGTTCAAGATTGGAAAGACGGCGGACGAAGCAGAACCCATGAACCCTAATCCTGCTGGAATCCAACGCTACCTTGTCGCAGTCGAATATGTTGGAACTCGCTTTTTCGGATCTCAGAAGCAACCCAATTGCCGTACGGTGGTTGGCGCGCTTGAGGAAGCGTTTCAGAAATTCATCGGCCAATCAGTTCCAGTGTTTTGTTCTAGCAGAACTGATGCTGGAGTTCATGCCTTATCAAATGCTTTTCATGTCGATGTTGAGCGCATAAGCAAAAGAAAGCCCGGAGAAGTGTTACCTCCTCATGAACCAGGAGTCGTTCGTAGGGCTGTGAATCATTTCTTACAGAAACAAGGTGATGTTATGGTAGTCGATGTTCGAAGTGTTCCCAATGATTTTCATGCTAGATATAAAGCTCAAGAGCGTACCTACTTTTATCGGTTAGTTTCTGGGGAAGACGTATTGTCAAGCTTTGTGAAGGATCGGGCATGGCACGTGCCAGAGACATTGAATATTGGGGCCATGAAGGAGGCGTGCAAAGTGCTTGTTGGGCATCATGACTTTAGCTCCTTCAGGGCAGCCGGTTGTCAGGCAAACTCGCCAATCCGAACTCTGGATGAACTGGACATTTGCGAAGTAGCTTCAACGCCTTATTTTCCAACATTAACCGAGCGAAACCAATGGCAAGGTAAAGACGAGACTTGCGAGGTTGGATTTGGGATGAGGAGAAGGCATCAATGTTTTGTGGTGACCGCACGAGCACGCTCTTTTCTGTATCATCAGGTGAGACTGATGGTTGGAGTCCTTAAAGCGGTTGGCACAGGAAATCTAACAGTAGGAGATGTAGAAAGAATCTTAGAAGCTAAGAGTGTGAATTCAACAAGTGCAATGGCCCCTGCATGCGGACTCTACCTCGGACATGTCAAATATGATCTACCAAACTGA

Protein sequence

MTENQKHRLEFSSDSSPERPFKIGKTADEAEPMNPNPAGIQRYLVAVEYVGTRFFGSQKQPNCRTVVGALEEAFQKFIGQSVPVFCSSRTDAGVHALSNAFHVDVERISKRKPGEVLPPHEPGVVRRAVNHFLQKQGDVMVVDVRSVPNDFHARYKAQERTYFYRLVSGEDVLSSFVKDRAWHVPETLNIGAMKEACKVLVGHHDFSSFRAAGCQANSPIRTLDELDICEVASTPYFPTLTERNQWQGKDETCEVGFGMRRRHQCFVVTARARSFLYHQVRLMVGVLKAVGTGNLTVGDVERILEAKSVNSTSAMAPACGLYLGHVKYDLPN

Homology

[Close]

BLAST of Sed0027668 vs. NCBI nr
Match: XP_038877867.1 (tRNA pseudouridine synthase A isoform X2 [Benincasa hispida])

HSP 1 Score: 579.3 bits (1492), Expect = 2.1e-161
Identity = 292/348 (83.91%), Postives = 306/348 (87.93%), Query Frame = 0


Query: 1   MTENQKHRLEFSSDSSPERPFKIGKTAD--------EAEPMNPNPAGIQRYLVAVEYVGT 60
           MTENQKH LEFS DSSP+RP KIGKT D        EAE MN NP GIQRYLVAVEY+GT
Sbjct: 1   MTENQKHGLEFSIDSSPQRPLKIGKTTDEVLNRHEEEAETMNRNP-GIQRYLVAVEYIGT 60

Query: 61  RFFGSQKQPNCRTVVGALEEAFQKFIGQSVPVFCSSRTDAGVHALSNAFHVDVERISKRK 120
           RF GSQ+QPNCRTVVGALEEAFQKFIGQ V VFCSSRTDAGVHALSN  HVDVERISKRK
Sbjct: 61  RFSGSQQQPNCRTVVGALEEAFQKFIGQPVSVFCSSRTDAGVHALSNVCHVDVERISKRK 120

Query: 121 PGEVLPPHEPGVVRRAVNHFLQK-QGDVMVVDVRSVPNDFHARYKAQERTYFYRLVSGED 180
           PGEVLPPHEPGVV RAVNHFLQK +GDV VVDVRSVPNDFHARYKAQERTYFYRLVSG +
Sbjct: 121 PGEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPE 180

Query: 181 VLSSFVKDRAWHVPETLNIGAMKEACKVLVGHHDFSSFRAAGCQANSPIRTLDELDICEV 240
            LSSF KDRAWHV ETLNIGAM++ACKVLVGHHDFSSFRAAGCQANSPIRTLDELD+CEV
Sbjct: 181 ALSSFEKDRAWHVSETLNIGAMQDACKVLVGHHDFSSFRAAGCQANSPIRTLDELDVCEV 240

Query: 241 ASTPYFPTLTERNQWQGKD--------ETCEVGFGMRRRHQCFVVTARARSFLYHQVRLM 300
            STPYFPT  ER Q QGK+        ETCEVGFG+RRRH+CFVVTARARSFLYHQVRLM
Sbjct: 241 VSTPYFPTSMERKQRQGKEGDSEVPHSETCEVGFGIRRRHRCFVVTARARSFLYHQVRLM 300

Query: 301 VGVLKAVGTGNLTVGDVERILEAKSVNSTSAMAPACGLYLGHVKYDLP 332
           VGVLKAVGTG+LTVGDVERILEAK+VNS S MAPACGLYLGHV+YDLP
Sbjct: 301 VGVLKAVGTGDLTVGDVERILEAKNVNSASPMAPACGLYLGHVRYDLP 347

[Close]

BLAST of Sed0027668 vs. NCBI nr
Match: XP_008445970.1 (PREDICTED: tRNA pseudouridine synthase A isoform X1 [Cucumis melo] >KAA0034150.1 tRNA pseudouridine synthase A isoform X1 [Cucumis melo var. makuwa] >TYK15769.1 tRNA pseudouridine synthase A isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 574.7 bits (1480), Expect = 5.1e-160
Identity = 290/349 (83.09%), Postives = 305/349 (87.39%), Query Frame = 0


Query: 1   MTENQKHRLEFSSDSSPERPFKIGKTAD--------EAEPMNPNPAGIQRYLVAVEYVGT 60
           MTENQKH LE S DSS +RP KIGKT D        EAE MNP P GIQRYLVAVEY+GT
Sbjct: 1   MTENQKHGLELSIDSSSQRPLKIGKTTDQVFHGDEEEAETMNPKP-GIQRYLVAVEYIGT 60

Query: 61  RFFGSQKQPNCRTVVGALEEAFQKFIGQSVPVFCSSRTDAGVHALSNAFHVDVERISKRK 120
           RF GSQ+QPNCRTVVGALEEAFQKFIGQ V VFCSSRTDAGVHALSN  HVDVERISKRK
Sbjct: 61  RFSGSQQQPNCRTVVGALEEAFQKFIGQPVSVFCSSRTDAGVHALSNVCHVDVERISKRK 120

Query: 121 PGEVLPPHEPGVVRRAVNHFLQK-QGDVMVVDVRSVPNDFHARYKAQERTYFYRLVSGED 180
           PGEVLPPHEPGVV +AVNHFLQK +GDV VVDVRSVPNDFHARYKAQERTYFYRLVSG +
Sbjct: 121 PGEVLPPHEPGVVHKAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPE 180

Query: 181 VLSSFVKDRAWHVPETLNIGAMKEACKVLVGHHDFSSFRAAGCQANSPIRTLDELDICEV 240
            LSSF KDRAWHVPETLNIGAM+EACKVLVGHHDFSSFRAAGCQANSPIRTLDEL++CEV
Sbjct: 181 ALSSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPIRTLDELNVCEV 240

Query: 241 ASTPYFPTLTERNQWQGKD---------ETCEVGFGMRRRHQCFVVTARARSFLYHQVRL 300
           ASTPYFPT  ER Q QGK+         ETCEVGFG+RRRH+CFVVTARARSFLYHQVRL
Sbjct: 241 ASTPYFPTSMERKQRQGKEESENEEPHSETCEVGFGIRRRHRCFVVTARARSFLYHQVRL 300

Query: 301 MVGVLKAVGTGNLTVGDVERILEAKSVNSTSAMAPACGLYLGHVKYDLP 332
           MVGVLKAVGTG+LTVGDVERILEAK+V+S   MAPACGLYLGHVKYDLP
Sbjct: 301 MVGVLKAVGTGDLTVGDVERILEAKNVSSARPMAPACGLYLGHVKYDLP 348

[Close]

BLAST of Sed0027668 vs. NCBI nr
Match: XP_011655540.1 (uncharacterized protein LOC101221274 isoform X1 [Cucumis sativus] >KGN51629.1 hypothetical protein Csa_008571 [Cucumis sativus])

HSP 1 Score: 572.4 bits (1474), Expect = 2.5e-159
Identity = 288/349 (82.52%), Postives = 305/349 (87.39%), Query Frame = 0


Query: 1   MTENQKHRLEFSSDSSPERPFKIGKTAD--------EAEPMNPNPAGIQRYLVAVEYVGT 60
           MTENQKH LE S DSS +RP KIGKT D        EAE MNP P GIQRYLVAVEY+GT
Sbjct: 1   MTENQKHGLELSIDSSSQRPLKIGKTTDELFHGDEEEAETMNPKP-GIQRYLVAVEYIGT 60

Query: 61  RFFGSQKQPNCRTVVGALEEAFQKFIGQSVPVFCSSRTDAGVHALSNAFHVDVERISKRK 120
           RF+GSQ+QPNCRTVVGALEEAFQKFIGQ V VFCSSRTDAGVHALSN  HVDVERISKRK
Sbjct: 61  RFYGSQQQPNCRTVVGALEEAFQKFIGQPVSVFCSSRTDAGVHALSNVCHVDVERISKRK 120

Query: 121 PGEVLPPHEPGVVRRAVNHFLQK-QGDVMVVDVRSVPNDFHARYKAQERTYFYRLVSGED 180
           PGEVLPPHEPGVV +AVNHFLQK +GDV VVDVRSVPNDFHARYKAQERTYFYRLVSG +
Sbjct: 121 PGEVLPPHEPGVVHKAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPE 180

Query: 181 VLSSFVKDRAWHVPETLNIGAMKEACKVLVGHHDFSSFRAAGCQANSPIRTLDELDICEV 240
            LSSF KDRAWHVPETLNIGAM+EACKVLVGHHDFSSFRAAGCQANSPIRTLDEL++CEV
Sbjct: 181 ALSSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPIRTLDELNVCEV 240

Query: 241 ASTPYFPTLTERNQWQGKD---------ETCEVGFGMRRRHQCFVVTARARSFLYHQVRL 300
           ASTPYFPT  ER Q QGK+         ETCEVGFG+RRRH+CFVVTARARSFLYHQVRL
Sbjct: 241 ASTPYFPTSMERKQRQGKEESEIEVPCSETCEVGFGIRRRHRCFVVTARARSFLYHQVRL 300

Query: 301 MVGVLKAVGTGNLTVGDVERILEAKSVNSTSAMAPACGLYLGHVKYDLP 332
           MVGVLKAVG+G+LTVGDV RILEAK+V+S   MAPACGLYLGHVKYDLP
Sbjct: 301 MVGVLKAVGSGDLTVGDVGRILEAKNVSSARPMAPACGLYLGHVKYDLP 348

[Close]

BLAST of Sed0027668 vs. NCBI nr
Match: XP_023524389.1 (uncharacterized protein LOC111788293 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 572.0 bits (1473), Expect = 3.3e-159
Identity = 288/349 (82.52%), Postives = 306/349 (87.68%), Query Frame = 0


Query: 1   MTENQKHRLEFSSDSSPERPFKIGKTA--------DEAEPMNPNPAGIQRYLVAVEYVGT 60
           MTENQKHRLEFSSDSSP+RP KIGKT         +EAE MN NP GIQRYLVAVEY+GT
Sbjct: 1   MTENQKHRLEFSSDSSPQRPLKIGKTTNEDLPGDKEEAESMNRNP-GIQRYLVAVEYIGT 60

Query: 61  RFFGSQKQPNCRTVVGALEEAFQKFIGQSVPVFCSSRTDAGVHALSNAFHVDVERISKRK 120
           RF GSQ+QPNCRTVVG LEEAF+KFIGQSV VFCSSRTDAGVHALSN  HVDVERISKRK
Sbjct: 61  RFSGSQQQPNCRTVVGVLEEAFRKFIGQSVSVFCSSRTDAGVHALSNVCHVDVERISKRK 120

Query: 121 PGEVLPPHEPGVVRRAVNHFLQK-QGDVMVVDVRSVPNDFHARYKAQERTYFYRLVSGED 180
           PGEVLPPHEPGVVRRAVNHFLQK  GDV VVDVRSVPND+HARYKAQERTYFYRLVSG +
Sbjct: 121 PGEVLPPHEPGVVRRAVNHFLQKNDGDVTVVDVRSVPNDYHARYKAQERTYFYRLVSGPE 180

Query: 181 VLSSFVKDRAWHVPETLNIGAMKEACKVLVGHHDFSSFRAAGCQANSPIRTLDELDICEV 240
            LSSF KDR WHVPE LNIGAM+EACKVLVGHHDFSSFRAAGCQANSP+RTLDELD+CEV
Sbjct: 181 TLSSFDKDRVWHVPEILNIGAMQEACKVLVGHHDFSSFRAAGCQANSPMRTLDELDVCEV 240

Query: 241 ASTPYFPTLTERNQWQGKD---------ETCEVGFGMRRRHQCFVVTARARSFLYHQVRL 300
            STPYFP+  ER Q QGK+         E+ EVGFG+RRRH+CFVVTARARSFLYHQVRL
Sbjct: 241 VSTPYFPSSMERKQRQGKEECENEVLHSESYEVGFGIRRRHRCFVVTARARSFLYHQVRL 300

Query: 301 MVGVLKAVGTGNLTVGDVERILEAKSVNSTSAMAPACGLYLGHVKYDLP 332
           MVGVLKAVGTG+LTVGDVERILEAK+V+S S MAPACGLYLGHVKYDLP
Sbjct: 301 MVGVLKAVGTGDLTVGDVERILEAKNVSSASPMAPACGLYLGHVKYDLP 348

[Close]

BLAST of Sed0027668 vs. NCBI nr
Match: XP_038877866.1 (tRNA pseudouridine synthase A isoform X1 [Benincasa hispida])

HSP 1 Score: 570.5 bits (1469), Expect = 9.7e-159
Identity = 292/360 (81.11%), Postives = 306/360 (85.00%), Query Frame = 0


Query: 1   MTENQKHRLEFSSDSSPERPFKIGKTAD--------EAEPMNPNPAGIQRYLVAVEYVGT 60
           MTENQKH LEFS DSSP+RP KIGKT D        EAE MN NP GIQRYLVAVEY+GT
Sbjct: 1   MTENQKHGLEFSIDSSPQRPLKIGKTTDEVLNRHEEEAETMNRNP-GIQRYLVAVEYIGT 60

Query: 61  RFFGSQKQPNCRTVVGA------------LEEAFQKFIGQSVPVFCSSRTDAGVHALSNA 120
           RF GSQ+QPNCRTVVGA            LEEAFQKFIGQ V VFCSSRTDAGVHALSN 
Sbjct: 61  RFSGSQQQPNCRTVVGALEVAEDDVLKMSLEEAFQKFIGQPVSVFCSSRTDAGVHALSNV 120

Query: 121 FHVDVERISKRKPGEVLPPHEPGVVRRAVNHFLQK-QGDVMVVDVRSVPNDFHARYKAQE 180
            HVDVERISKRKPGEVLPPHEPGVV RAVNHFLQK +GDV VVDVRSVPNDFHARYKAQE
Sbjct: 121 CHVDVERISKRKPGEVLPPHEPGVVHRAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQE 180

Query: 181 RTYFYRLVSGEDVLSSFVKDRAWHVPETLNIGAMKEACKVLVGHHDFSSFRAAGCQANSP 240
           RTYFYRLVSG + LSSF KDRAWHV ETLNIGAM++ACKVLVGHHDFSSFRAAGCQANSP
Sbjct: 181 RTYFYRLVSGPEALSSFEKDRAWHVSETLNIGAMQDACKVLVGHHDFSSFRAAGCQANSP 240

Query: 241 IRTLDELDICEVASTPYFPTLTERNQWQGKD--------ETCEVGFGMRRRHQCFVVTAR 300
           IRTLDELD+CEV STPYFPT  ER Q QGK+        ETCEVGFG+RRRH+CFVVTAR
Sbjct: 241 IRTLDELDVCEVVSTPYFPTSMERKQRQGKEGDSEVPHSETCEVGFGIRRRHRCFVVTAR 300

Query: 301 ARSFLYHQVRLMVGVLKAVGTGNLTVGDVERILEAKSVNSTSAMAPACGLYLGHVKYDLP 332
           ARSFLYHQVRLMVGVLKAVGTG+LTVGDVERILEAK+VNS S MAPACGLYLGHV+YDLP
Sbjct: 301 ARSFLYHQVRLMVGVLKAVGTGDLTVGDVERILEAKNVNSASPMAPACGLYLGHVRYDLP 359

[Close]

BLAST of Sed0027668 vs. ExPASy Swiss-Prot
Match: A9ILJ7 (tRNA pseudouridine synthase A OS=Bartonella tribocorum (strain CIP 105476 / IBS 506) OX=382640 GN=truA PE=3 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 1.7e-44
Identity = 107/290 (36.90%), Postives = 156/290 (53.79%), Query Frame = 0


Query: 40  IQRYLVAVEYVGTRFFGSQKQPNCRTVVGALEEAFQKFIGQSVPVFCSSRTDAGVHALSN 99
           + R+ + +EY G+ + G Q+Q    T+ GALE+A  +F GQ + +  + RTDAGVHA   
Sbjct: 1   MSRFKLTLEYDGSNYAGWQRQAKLHTIQGALEQAIFRFSGQQLTITTAGRTDAGVHATGQ 60

Query: 100 AFHVDVERISKRKPGEVLPPHEPGVVRRAVNHFLQKQGD-VMVVDVRSVPNDFHARYKAQ 159
             HVD  +          PPH    VR A+N  L++Q + + +++V +VP+DF AR+ A 
Sbjct: 61  VAHVDFIK--------NWPPH---TVRDALNALLRQQSEAISILNVENVPDDFDARFSAI 120

Query: 160 ERTYFYRLVSGEDVLSSFVKDRAWHVPETLNIGAMKEACKVLVGHHDFSSFRAAGCQANS 219
           +R Y +++++     +   K R W +P+ LN  AM EA + LVG HDF++FR+A CQA S
Sbjct: 121 KRHYLFKILNRRSPPALNAK-RVWWIPKPLNAQAMHEAAQKLVGQHDFTTFRSAHCQAKS 180

Query: 220 PIRTLDELDICEVASTPYFPTLTERNQWQGKDETCEVGFGMRRRHQCFVVTARARSFLYH 279
           PIRTL+ LDI                Q +G++                 + A+ARSFL+H
Sbjct: 181 PIRTLERLDI----------------QKEGEE---------------IFIYAKARSFLHH 240

Query: 280 QVRLMVGVLKAVGTGNLTVGDVERILEAKSVNSTSAMAPACGLYLGHVKY 329
           Q+R   G L  VG G  T  D+E  L AK       +AP  GLYL  V+Y
Sbjct: 241 QIRSFAGSLMEVGIGRWTAQDLEAALHAKDRKRCGVVAPPSGLYLTKVEY 247

[Close]

BLAST of Sed0027668 vs. ExPASy Swiss-Prot
Match: B6IPI0 (tRNA pseudouridine synthase A OS=Rhodospirillum centenum (strain ATCC 51521 / SW) OX=414684 GN=truA PE=3 SV=1)

HSP 1 Score: 179.5 bits (454), Expect = 6.3e-44
Identity = 114/289 (39.45%), Postives = 150/289 (51.90%), Query Frame = 0


Query: 40  IQRYLVAVEYVGTRFFGSQKQPNCRTVVGALEEAFQKFIGQSVPVFCSSRTDAGVHALSN 99
           +QR+ + +EY G  F G Q+Q N  +V  ALEEA  +F  ++V VFC+ RTDAGVHAL+ 
Sbjct: 1   MQRWKLTLEYDGRPFVGWQRQDNGPSVQQALEEAIYRFTQETVTVFCAGRTDAGVHALAM 60

Query: 100 AFHVDVERISKRKPGEVLPPHEPGVVRRAVNHFLQKQGDVMVVDVRSVPNDFHARYKAQE 159
              VD+ R +               V+ A+N  L K   V V+ V  V  DFHAR+    
Sbjct: 61  VASVDLAREATADK-----------VQAALNFHL-KPHPVAVLRVEPVAPDFHARFSCLG 120

Query: 160 RTYFYRLVSGEDVLSSFVKDRAWHVPETLNIGAMKEACKVLVGHHDFSSFRAAGCQANSP 219
           R Y YR+++     ++    R WHV   L+  AM EA + LVG HDFSSFRA+ CQA SP
Sbjct: 121 RAYLYRILN-RRAPAALEAGRVWHVQGGLDAEAMHEAAQRLVGQHDFSSFRASLCQAKSP 180

Query: 220 IRTLDELDICEVASTPYFPTLTERNQWQGKDETCEVGFGMRRRHQCFVVTARARSFLYHQ 279
           ++TL +L +                          VG  +R       + ARARSFL+HQ
Sbjct: 181 VKTLSDLSV------------------------ARVGEEVR-------IVARARSFLHHQ 240

Query: 280 VRLMVGVLKAVGTGNLTVGDVERILEAKSVNSTSAMAPACGLYLGHVKY 329
           VR MVG LK VG G  T  DV R L A++ ++    APA GLY     Y
Sbjct: 241 VRNMVGTLKLVGEGRWTADDVSRALAARNRSAAGPTAPAAGLYFTEAWY 245

[Close]

BLAST of Sed0027668 vs. ExPASy Swiss-Prot
Match: A7IKI9 (tRNA pseudouridine synthase A OS=Xanthobacter autotrophicus (strain ATCC BAA-1158 / Py2) OX=78245 GN=truA PE=3 SV=1)

HSP 1 Score: 179.1 bits (453), Expect = 8.2e-44
Identity = 111/287 (38.68%), Postives = 151/287 (52.61%), Query Frame = 0


Query: 42  RYLVAVEYVGTRFFGSQKQPNCRTVVGALEEAFQKFIGQSVPVFCSSRTDAGVHALSNAF 101
           RY + +EY GT + G Q Q + +TV GAL +AF++F G+ V V  + RTDAGVHA     
Sbjct: 3   RYKLTIEYDGTPYAGWQIQADQQTVQGALAQAFKRFCGEDVHVAGAGRTDAGVHATGQVA 62

Query: 102 HVDVERISKRKPGEVLPPHEPGVVRRAVNHFLQKQGDVMVVDVRSVPNDFHARYKAQERT 161
           HVD+ R                 VR A+   L+    + V+    VP+ F AR+ A +R 
Sbjct: 63  HVDLSR-----------HWRSDTVRDAMTAQLRPH-PIAVLSAEEVPDSFDARFSATKRH 122

Query: 162 YFYRLVSGEDVLSSFVKDRAWHVPETLNIGAMKEACKVLVGHHDFSSFRAAGCQANSPIR 221
           Y YR+++    L +  +DRAW +P+ L+  AM EA + L+G HDFS+FRAA CQA SP++
Sbjct: 123 YLYRIINRRPDL-ALDRDRAWRIPQKLDAQAMHEAAQRLLGKHDFSTFRAAECQAASPLK 182

Query: 222 TLDELDICEVASTPYFPTLTERNQWQGKDETCEVGFGMRRRHQCFVVTARARSFLYHQVR 281
           TLD+LD+                               R   +  V+T+ ARSFL+HQVR
Sbjct: 183 TLDQLDV------------------------------SRSGDEIRVITS-ARSFLHHQVR 242

Query: 282 LMVGVLKAVGTGNLTVGDVERILEAKSVNSTSAMAPACGLYLGHVKY 329
            MVG L  VG G  +  D+E  L+A        MAPA GLYL  V Y
Sbjct: 243 SMVGSLMRVGEGRWSADDLEAALKAADRTRCGPMAPAAGLYLAAVSY 245

[Close]

BLAST of Sed0027668 vs. ExPASy Swiss-Prot
Match: Q6G547 (tRNA pseudouridine synthase A OS=Bartonella henselae (strain ATCC 49882 / DSM 28221 / Houston 1) OX=283166 GN=truA PE=3 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 1.1e-43
Identity = 106/288 (36.81%), Postives = 151/288 (52.43%), Query Frame = 0


Query: 42  RYLVAVEYVGTRFFGSQKQPNCRTVVGALEEAFQKFIGQSVPVFCSSRTDAGVHALSNAF 101
           R+ + +EY G+ + G Q+Q   RT+  ALE+A   F GQ + +  + RTDAGVHA     
Sbjct: 3   RFKLTLEYDGSNYAGWQRQAELRTIQSALEQALFHFSGQQLTITTAGRTDAGVHATGQVA 62

Query: 102 HVDVERISKRKPGEVLPPHEPGVVRRAVNHFLQKQGD-VMVVDVRSVPNDFHARYKAQER 161
           HVD E+  +              VR A+N  LQKQGD + ++ V++VP+ F AR+ A +R
Sbjct: 63  HVDFEKNWRTH-----------TVRDALNAHLQKQGDNIAILHVQNVPDSFDARFSAIKR 122

Query: 162 TYFYRLVSGEDVLSSFVKDRAWHVPETLNIGAMKEACKVLVGHHDFSSFRAAGCQANSPI 221
            Y +++++     +   K R W +P+ LN  AM EA + LVG HDF++FR+A CQA SPI
Sbjct: 123 HYLFKILNRRSPPALNTK-RVWWIPKPLNAQAMHEAAQKLVGKHDFTTFRSAHCQAKSPI 182

Query: 222 RTLDELDICEVASTPYFPTLTERNQWQGKDETCEVGFGMRRRHQCFVVTARARSFLYHQV 281
           RTL+ LD+                               +R  +   + A+ARSFL+HQ+
Sbjct: 183 RTLERLDV-------------------------------QREGEEIFLYAQARSFLHHQI 242

Query: 282 RLMVGVLKAVGTGNLTVGDVERILEAKSVNSTSAMAPACGLYLGHVKY 329
           R   G L  VG G  T  D+E  L AK       +AP  GLYL  V+Y
Sbjct: 243 RSFAGSLMEVGIGRWTTQDLEAALHAKDRTRCGMVAPPSGLYLTKVEY 247

[Close]

BLAST of Sed0027668 vs. ExPASy Swiss-Prot
Match: A7HPQ3 (tRNA pseudouridine synthase A OS=Parvibaculum lavamentivorans (strain DS-1 / DSM 13023 / NCIMB 13966) OX=402881 GN=truA PE=3 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 1.1e-43
Identity = 109/288 (37.85%), Postives = 151/288 (52.43%), Query Frame = 0


Query: 42  RYLVAVEYVGTRFFGSQKQPNCRTVVGALEEAFQKFIGQSVPVFCSSRTDAGVHALSNAF 101
           RY + +EY G+ F G Q Q N R+V   LEE  + F GQ + +F + RTDAGVHAL    
Sbjct: 3   RYKLTIEYDGSPFVGWQAQTNGRSVQQVLEEGIKGFCGQELKIFGAGRTDAGVHALGQVA 62

Query: 102 HVDVERISKRKPGEVLPPHEPGVVRRAVNHFLQKQGDVMVVDVRSVPNDFHARYKAQERT 161
           HVD E            P  P  +R AVN  ++    V +V+   VP+ F AR+ A +R 
Sbjct: 63  HVDFE-----------TPVAPDTLRDAVNAHMRPH-PVAIVEAEEVPDSFEARFSAVKRH 122

Query: 162 YFYRLVSGEDVLSSFVKDRAWHVPETLNIGAMKEACKVLVGHHDFSSFRAAGCQANSPIR 221
           Y YR+V+    L +  + +AW V +TL+  AM +A + LVG+HDF++FR+  CQA SP++
Sbjct: 123 YMYRIVNRRAPL-TLDRGQAWLVHKTLDADAMHDAAQALVGNHDFTTFRSVQCQAKSPVK 182

Query: 222 TLDELDICEVASTPYFPTLTERNQWQGKDETCEVGFGMRRRHQCFVVTARARSFLYHQVR 281
           T+DE+ +   A                 DE                +  RARSFL++QVR
Sbjct: 183 TVDEISVSRYA-----------------DE--------------IEIVCRARSFLHNQVR 242

Query: 282 LMVGVLKAVGTGNLTVGDVERILEAKSVNSTSAMAPACGLYLGHVKYD 330
             VG LK VG G+ T   VE+ L AK   +   +AP  GLYL  V Y+
Sbjct: 243 SFVGTLKMVGDGSWTRRKVEKALAAKDRAACGPVAPPDGLYLLQVDYE 246

[Close]

BLAST of Sed0027668 vs. ExPASy TrEMBL
Match: A0A5D3CZZ6 (tRNA pseudouridine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G002730 PE=3 SV=1)

HSP 1 Score: 574.7 bits (1480), Expect = 2.5e-160
Identity = 290/349 (83.09%), Postives = 305/349 (87.39%), Query Frame = 0


Query: 1   MTENQKHRLEFSSDSSPERPFKIGKTAD--------EAEPMNPNPAGIQRYLVAVEYVGT 60
           MTENQKH LE S DSS +RP KIGKT D        EAE MNP P GIQRYLVAVEY+GT
Sbjct: 1   MTENQKHGLELSIDSSSQRPLKIGKTTDQVFHGDEEEAETMNPKP-GIQRYLVAVEYIGT 60

Query: 61  RFFGSQKQPNCRTVVGALEEAFQKFIGQSVPVFCSSRTDAGVHALSNAFHVDVERISKRK 120
           RF GSQ+QPNCRTVVGALEEAFQKFIGQ V VFCSSRTDAGVHALSN  HVDVERISKRK
Sbjct: 61  RFSGSQQQPNCRTVVGALEEAFQKFIGQPVSVFCSSRTDAGVHALSNVCHVDVERISKRK 120

Query: 121 PGEVLPPHEPGVVRRAVNHFLQK-QGDVMVVDVRSVPNDFHARYKAQERTYFYRLVSGED 180
           PGEVLPPHEPGVV +AVNHFLQK +GDV VVDVRSVPNDFHARYKAQERTYFYRLVSG +
Sbjct: 121 PGEVLPPHEPGVVHKAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPE 180

Query: 181 VLSSFVKDRAWHVPETLNIGAMKEACKVLVGHHDFSSFRAAGCQANSPIRTLDELDICEV 240
            LSSF KDRAWHVPETLNIGAM+EACKVLVGHHDFSSFRAAGCQANSPIRTLDEL++CEV
Sbjct: 181 ALSSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPIRTLDELNVCEV 240

Query: 241 ASTPYFPTLTERNQWQGKD---------ETCEVGFGMRRRHQCFVVTARARSFLYHQVRL 300
           ASTPYFPT  ER Q QGK+         ETCEVGFG+RRRH+CFVVTARARSFLYHQVRL
Sbjct: 241 ASTPYFPTSMERKQRQGKEESENEEPHSETCEVGFGIRRRHRCFVVTARARSFLYHQVRL 300

Query: 301 MVGVLKAVGTGNLTVGDVERILEAKSVNSTSAMAPACGLYLGHVKYDLP 332
           MVGVLKAVGTG+LTVGDVERILEAK+V+S   MAPACGLYLGHVKYDLP
Sbjct: 301 MVGVLKAVGTGDLTVGDVERILEAKNVSSARPMAPACGLYLGHVKYDLP 348

[Close]

BLAST of Sed0027668 vs. ExPASy TrEMBL
Match: A0A1S3BDG9 (tRNA pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103488841 PE=3 SV=1)

HSP 1 Score: 574.7 bits (1480), Expect = 2.5e-160
Identity = 290/349 (83.09%), Postives = 305/349 (87.39%), Query Frame = 0


Query: 1   MTENQKHRLEFSSDSSPERPFKIGKTAD--------EAEPMNPNPAGIQRYLVAVEYVGT 60
           MTENQKH LE S DSS +RP KIGKT D        EAE MNP P GIQRYLVAVEY+GT
Sbjct: 1   MTENQKHGLELSIDSSSQRPLKIGKTTDQVFHGDEEEAETMNPKP-GIQRYLVAVEYIGT 60

Query: 61  RFFGSQKQPNCRTVVGALEEAFQKFIGQSVPVFCSSRTDAGVHALSNAFHVDVERISKRK 120
           RF GSQ+QPNCRTVVGALEEAFQKFIGQ V VFCSSRTDAGVHALSN  HVDVERISKRK
Sbjct: 61  RFSGSQQQPNCRTVVGALEEAFQKFIGQPVSVFCSSRTDAGVHALSNVCHVDVERISKRK 120

Query: 121 PGEVLPPHEPGVVRRAVNHFLQK-QGDVMVVDVRSVPNDFHARYKAQERTYFYRLVSGED 180
           PGEVLPPHEPGVV +AVNHFLQK +GDV VVDVRSVPNDFHARYKAQERTYFYRLVSG +
Sbjct: 121 PGEVLPPHEPGVVHKAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPE 180

Query: 181 VLSSFVKDRAWHVPETLNIGAMKEACKVLVGHHDFSSFRAAGCQANSPIRTLDELDICEV 240
            LSSF KDRAWHVPETLNIGAM+EACKVLVGHHDFSSFRAAGCQANSPIRTLDEL++CEV
Sbjct: 181 ALSSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPIRTLDELNVCEV 240

Query: 241 ASTPYFPTLTERNQWQGKD---------ETCEVGFGMRRRHQCFVVTARARSFLYHQVRL 300
           ASTPYFPT  ER Q QGK+         ETCEVGFG+RRRH+CFVVTARARSFLYHQVRL
Sbjct: 241 ASTPYFPTSMERKQRQGKEESENEEPHSETCEVGFGIRRRHRCFVVTARARSFLYHQVRL 300

Query: 301 MVGVLKAVGTGNLTVGDVERILEAKSVNSTSAMAPACGLYLGHVKYDLP 332
           MVGVLKAVGTG+LTVGDVERILEAK+V+S   MAPACGLYLGHVKYDLP
Sbjct: 301 MVGVLKAVGTGDLTVGDVERILEAKNVSSARPMAPACGLYLGHVKYDLP 348

[Close]

BLAST of Sed0027668 vs. ExPASy TrEMBL
Match: A0A0A0KRX0 (tRNA pseudouridine synthase OS=Cucumis sativus OX=3659 GN=Csa_5G586020 PE=3 SV=1)

HSP 1 Score: 572.4 bits (1474), Expect = 1.2e-159
Identity = 288/349 (82.52%), Postives = 305/349 (87.39%), Query Frame = 0


Query: 1   MTENQKHRLEFSSDSSPERPFKIGKTAD--------EAEPMNPNPAGIQRYLVAVEYVGT 60
           MTENQKH LE S DSS +RP KIGKT D        EAE MNP P GIQRYLVAVEY+GT
Sbjct: 1   MTENQKHGLELSIDSSSQRPLKIGKTTDELFHGDEEEAETMNPKP-GIQRYLVAVEYIGT 60

Query: 61  RFFGSQKQPNCRTVVGALEEAFQKFIGQSVPVFCSSRTDAGVHALSNAFHVDVERISKRK 120
           RF+GSQ+QPNCRTVVGALEEAFQKFIGQ V VFCSSRTDAGVHALSN  HVDVERISKRK
Sbjct: 61  RFYGSQQQPNCRTVVGALEEAFQKFIGQPVSVFCSSRTDAGVHALSNVCHVDVERISKRK 120

Query: 121 PGEVLPPHEPGVVRRAVNHFLQK-QGDVMVVDVRSVPNDFHARYKAQERTYFYRLVSGED 180
           PGEVLPPHEPGVV +AVNHFLQK +GDV VVDVRSVPNDFHARYKAQERTYFYRLVSG +
Sbjct: 121 PGEVLPPHEPGVVHKAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPE 180

Query: 181 VLSSFVKDRAWHVPETLNIGAMKEACKVLVGHHDFSSFRAAGCQANSPIRTLDELDICEV 240
            LSSF KDRAWHVPETLNIGAM+EACKVLVGHHDFSSFRAAGCQANSPIRTLDEL++CEV
Sbjct: 181 ALSSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPIRTLDELNVCEV 240

Query: 241 ASTPYFPTLTERNQWQGKD---------ETCEVGFGMRRRHQCFVVTARARSFLYHQVRL 300
           ASTPYFPT  ER Q QGK+         ETCEVGFG+RRRH+CFVVTARARSFLYHQVRL
Sbjct: 241 ASTPYFPTSMERKQRQGKEESEIEVPCSETCEVGFGIRRRHRCFVVTARARSFLYHQVRL 300

Query: 301 MVGVLKAVGTGNLTVGDVERILEAKSVNSTSAMAPACGLYLGHVKYDLP 332
           MVGVLKAVG+G+LTVGDV RILEAK+V+S   MAPACGLYLGHVKYDLP
Sbjct: 301 MVGVLKAVGSGDLTVGDVGRILEAKNVSSARPMAPACGLYLGHVKYDLP 348

[Close]

BLAST of Sed0027668 vs. ExPASy TrEMBL
Match: A0A6J1KFZ8 (tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111492882 PE=3 SV=1)

HSP 1 Score: 570.1 bits (1468), Expect = 6.1e-159
Identity = 287/349 (82.23%), Postives = 305/349 (87.39%), Query Frame = 0


Query: 1   MTENQKHRLEFSSDSSPERPFKIGKTA--------DEAEPMNPNPAGIQRYLVAVEYVGT 60
           MTENQKHRLEFSSDSSP+RP KIGKT         +EAE MN NP GIQRYLVAVEY+GT
Sbjct: 1   MTENQKHRLEFSSDSSPQRPLKIGKTTNEDLPGDKEEAESMNRNP-GIQRYLVAVEYIGT 60

Query: 61  RFFGSQKQPNCRTVVGALEEAFQKFIGQSVPVFCSSRTDAGVHALSNAFHVDVERISKRK 120
           RF GSQ+QPNCRTVVG LEEAF+KFIGQSV VFCSSRTDAGVHALSN  HVDVERISKRK
Sbjct: 61  RFSGSQQQPNCRTVVGVLEEAFRKFIGQSVSVFCSSRTDAGVHALSNVCHVDVERISKRK 120

Query: 121 PGEVLPPHEPGVVRRAVNHFLQK-QGDVMVVDVRSVPNDFHARYKAQERTYFYRLVSGED 180
           PGEVLPPHEPGVVRRAVNHFLQK  GDV VVDVRSVPND+HARYKAQERTYFYRLVSG +
Sbjct: 121 PGEVLPPHEPGVVRRAVNHFLQKNDGDVTVVDVRSVPNDYHARYKAQERTYFYRLVSGPE 180

Query: 181 VLSSFVKDRAWHVPETLNIGAMKEACKVLVGHHDFSSFRAAGCQANSPIRTLDELDICEV 240
            LSSF KDR WHVPE LNIGAM+EACKVLVGHHDFSSFRAAGCQANSP+RTLDELD+CEV
Sbjct: 181 TLSSFDKDRVWHVPEILNIGAMQEACKVLVGHHDFSSFRAAGCQANSPMRTLDELDVCEV 240

Query: 241 ASTPYFPTLTERNQWQGKD---------ETCEVGFGMRRRHQCFVVTARARSFLYHQVRL 300
            STPYFP+  ER Q QGK+         E+ EVGFG+RRRH+CFVVTARARSFLYHQVRL
Sbjct: 241 ISTPYFPSSMERKQRQGKEEGENEVLHSESYEVGFGIRRRHRCFVVTARARSFLYHQVRL 300

Query: 301 MVGVLKAVGTGNLTVGDVERILEAKSVNSTSAMAPACGLYLGHVKYDLP 332
           MVGVLK VGTG+LTVGDVERILEAK+V+S S MAPACGLYLGHVKYDLP
Sbjct: 301 MVGVLKVVGTGDLTVGDVERILEAKNVSSASPMAPACGLYLGHVKYDLP 348

[Close]

BLAST of Sed0027668 vs. ExPASy TrEMBL
Match: A0A6J1GY72 (tRNA pseudouridine synthase OS=Cucurbita moschata OX=3662 GN=LOC111458281 PE=3 SV=1)

HSP 1 Score: 569.7 bits (1467), Expect = 8.0e-159
Identity = 287/349 (82.23%), Postives = 305/349 (87.39%), Query Frame = 0


Query: 1   MTENQKHRLEFSSDSSPERPFKIGKTA--------DEAEPMNPNPAGIQRYLVAVEYVGT 60
           MTENQKHRLEFSSDSSP+RP KIGKT         +EAE MN NP GIQRYLVAVEY+GT
Sbjct: 1   MTENQKHRLEFSSDSSPQRPLKIGKTTNEDLLGDKEEAESMNRNP-GIQRYLVAVEYIGT 60

Query: 61  RFFGSQKQPNCRTVVGALEEAFQKFIGQSVPVFCSSRTDAGVHALSNAFHVDVERISKRK 120
           RF GSQ+QPNCRTVVG LEEAF+KFIGQSV VFCSSRTDAGVHALSN  HVDVERISKRK
Sbjct: 61  RFSGSQQQPNCRTVVGVLEEAFRKFIGQSVSVFCSSRTDAGVHALSNVCHVDVERISKRK 120

Query: 121 PGEVLPPHEPGVVRRAVNHFLQK-QGDVMVVDVRSVPNDFHARYKAQERTYFYRLVSGED 180
           PGEVLPPHEP VVRRAVNHFLQK  GDV VVDVRSVPND+HARYKAQERTYFYRLVSG +
Sbjct: 121 PGEVLPPHEPSVVRRAVNHFLQKNDGDVTVVDVRSVPNDYHARYKAQERTYFYRLVSGPE 180

Query: 181 VLSSFVKDRAWHVPETLNIGAMKEACKVLVGHHDFSSFRAAGCQANSPIRTLDELDICEV 240
            LSSF KDR WHVPE LNIGAM+EACKVLVGHHDFSSFRAAGCQANSP+RTLDELD+CEV
Sbjct: 181 TLSSFDKDRVWHVPEILNIGAMQEACKVLVGHHDFSSFRAAGCQANSPMRTLDELDVCEV 240

Query: 241 ASTPYFPTLTERNQWQGKD---------ETCEVGFGMRRRHQCFVVTARARSFLYHQVRL 300
            STPYFP+  ER Q QGK+         E+ EVGFG+RRRH+CFVVTARARSFLYHQVRL
Sbjct: 241 VSTPYFPSSMERKQRQGKEEGENGVLHSESYEVGFGIRRRHRCFVVTARARSFLYHQVRL 300

Query: 301 MVGVLKAVGTGNLTVGDVERILEAKSVNSTSAMAPACGLYLGHVKYDLP 332
           MVGVLKAVGTG+LTVGDVERILEAK+V+S S MAPACGLYLGHVKYDLP
Sbjct: 301 MVGVLKAVGTGDLTVGDVERILEAKNVSSASPMAPACGLYLGHVKYDLP 348

[Close]

BLAST of Sed0027668 vs. TAIR 10
Match: AT1G09800.1 (Pseudouridine synthase family protein )

HSP 1 Score: 440.3 bits (1131), Expect = 1.4e-123
Identity = 233/372 (62.63%), Postives = 270/372 (72.58%), Query Frame = 0


Query: 1   MTENQKHRLE-FSSDSSPERPFKIGKT---ADE----------AEPMNPNPAGIQRYLVA 60
           M++N KH L+  +S+ S ER  K+ +    +DE           E MNP    +QRYLVA
Sbjct: 1   MSDNLKHPLDCTASEESLERLSKVARIEGFSDEESEEQQGLADLESMNPVNPRVQRYLVA 60

Query: 61  VEYVGTRFFGSQKQPNCRTVVGALEEAFQKFIGQSVPVFCSSRTDAGVHALSNAFHVDVE 120
           +EY+GTRF GSQ+Q   RTVVG L+EAF KFIGQ V +FCSSRTDAGVHALSN  HVDVE
Sbjct: 61  IEYIGTRFSGSQQQAKDRTVVGVLQEAFHKFIGQPVKIFCSSRTDAGVHALSNVCHVDVE 120

Query: 121 RISKRKPGEVLPPHEPGVVRRAVNHFLQ-KQGDVMVVDVRSVPNDFHARYKAQERTYFYR 180
           RISKRKPGEVLPPHEPGVV+RAVNHFLQ K GDVMV+DVR VP+++HARYKAQERTYFYR
Sbjct: 121 RISKRKPGEVLPPHEPGVVQRAVNHFLQRKDGDVMVIDVRCVPSNYHARYKAQERTYFYR 180

Query: 181 LVSGEDVLSSFVKDRAWHVPETLNIGAMKEACKVLVGHHDFSSFRAAGCQANSPIRTLDE 240
           L+SG D LS   KDR WHVPE LN+  M+EAC+VLVG HDFSSFRAAGCQA SP+R+LDE
Sbjct: 181 LLSGSDPLSILEKDRCWHVPEELNLRFMQEACRVLVGSHDFSSFRAAGCQAKSPMRSLDE 240

Query: 241 LDICEVASTPYFPTLTERN------------QWQGKDETCEV--------------GFGM 300
           L+I EV STPYFP++TER               Q K ET  V               FG 
Sbjct: 241 LNITEVPSTPYFPSITERTLSNLNNGDPLACSNQPKSETAGVTTNVGEVEGSTEGDSFGR 300

Query: 301 RRRHQCFVVTARARSFLYHQVRLMVGVLKAVGTGNLTVGDVERILEAKSVNSTSAMAPAC 332
           RRRH+C+VVTAR+R FLYHQVRL+VG LK VGTG LTV DVERILEAK+V++   MAPA 
Sbjct: 301 RRRHRCYVVTARSRGFLYHQVRLIVGALKCVGTGELTVSDVERILEAKTVSAAKPMAPAS 360

[Close]

BLAST of Sed0027668 vs. TAIR 10
Match: AT1G34150.1 (Pseudouridine synthase family protein )

HSP 1 Score: 70.9 bits (172), Expect = 2.2e-12
Identity = 77/315 (24.44%), Postives = 124/315 (39.37%), Query Frame = 0


Query: 6   KHRLEFSSDSSPERPFKIGKTADEAEPMNPNPAGIQRYL-VAVEYVGTRFFG----SQKQ 65
           KH    S  SS  R  ++ K    + P +      +RY+ + + Y G RF+G    +Q +
Sbjct: 57  KHDRSLSDSSSLVRRRRVRKGDKNSIPSH---LISKRYVALKIMYFGKRFYGFSAEAQME 116

Query: 66  PNCRTVVGALEEAFQKFIGQSVPVFCS--SRTDAGVHALSNAFHVDVERISKRKPGEVLP 125
           P+  + V    E  +  +G       S   RTD GV +      + +    K  PG+   
Sbjct: 117 PSIESEVFKALERTRLLVGDKKDSCYSRCGRTDKGVSSTGQVIALFLRSRLKSPPGDSKA 176

Query: 126 PHEPGVVRRAVNHFLQKQGDVMVVDVRSV-----PNDFHARYKAQERTYFYRLVSGEDVL 185
                   R    +++     +  D+R +     P DFHAR+    R Y Y         
Sbjct: 177 QVNGRTGERPEYDYVRVLNRALPDDIRVIGWSPAPIDFHARFSCYAREYKYFF------- 236

Query: 186 SSFVKDRAWHVPETLNIGAMKEACKVLVGHHDFSSFRAAGCQANSPIRTLDELDI-CEVA 245
                   W   + LN+ AM  A K  +G HDF +F    C+       +D  ++ C   
Sbjct: 237 --------WR--QNLNLSAMDFAGKKFIGEHDFRNF----CK-------MDVANVHCYTR 296

Query: 246 STPYFPTLTERNQWQGKDETCEVGFGMRRRHQCFVVTARARSFLYHQVRLMVGVLKAVGT 305
              +F     +N  +G D+ C               T R  +FL+HQ+R MV VL  +G 
Sbjct: 297 RVTFFEVSPCQNSHEG-DQLC-------------TFTMRGSAFLWHQIRCMVAVLFMIGQ 326

Query: 306 GNLTVGDVERILEAK 308
           G  +V  ++ +L+ K
Sbjct: 357 GVESVDVIDTLLDTK 326

[Close]

BLAST of Sed0027668 vs. TAIR 10
Match: AT5G35400.1 (Pseudouridine synthase family protein )

HSP 1 Score: 49.3 bits (116), Expect = 7.0e-06
Identity = 36/135 (26.67%), Postives = 53/135 (39.26%), Query Frame = 0


Query: 43  YLVAVEYVGTRFFGSQKQPNCRTVVGALEEAFQKFIGQSVP-------------VFCSSR 102
           + + + Y G  F G QKQP+  TV G +E++   F+ +                V  + R
Sbjct: 95  FKIVLSYNGASFDGWQKQPDLHTVQGVVEKSLGGFVDERKAQLLKKKCKPLEGRVLVAGR 154

Query: 103 TDAGVHALSNAFHVDVERISKRKPGEVLPPHEPGVVRRAVNHFLQKQGDVMVVDVRSVPN 162
           TD GV AL+        R             EP  +  A+N      G + VV +  V  
Sbjct: 155 TDKGVSALNQVCSFYTWR----------KDIEPIAIEDAINK--DASGKLRVVSISKVSR 214

Query: 163 DFHARYKAQERTYFY 165
            FH  + A+ R Y Y
Sbjct: 215 SFHPNFSAKWRRYLY 217

[Close]

BLAST of Sed0027668 vs. TAIR 10
Match: AT5G35400.2 (Pseudouridine synthase family protein )

HSP 1 Score: 49.3 bits (116), Expect = 7.0e-06
Identity = 36/135 (26.67%), Postives = 53/135 (39.26%), Query Frame = 0


Query: 43  YLVAVEYVGTRFFGSQKQPNCRTVVGALEEAFQKFIGQSVP-------------VFCSSR 102
           + + + Y G  F G QKQP+  TV G +E++   F+ +                V  + R
Sbjct: 95  FKIVLSYNGASFDGWQKQPDLHTVQGVVEKSLGGFVDERKAQLLKKKCKPLEGRVLVAGR 154

Query: 103 TDAGVHALSNAFHVDVERISKRKPGEVLPPHEPGVVRRAVNHFLQKQGDVMVVDVRSVPN 162
           TD GV AL+        R             EP  +  A+N      G + VV +  V  
Sbjct: 155 TDKGVSALNQVCSFYTWR----------KDIEPIAIEDAINK--DASGKLRVVSISKVSR 214

Query: 163 DFHARYKAQERTYFY 165
            FH  + A+ R Y Y
Sbjct: 215 SFHPNFSAKWRRYLY 217

The following BLAST results are available for this feature:

BLAST of Sed0027668 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038877867.1	2.1e-161	83.91	tRNA pseudouridine synthase A isoform X2 [Benincasa hispida]	[more]
XP_008445970.1	5.1e-160	83.09	PREDICTED: tRNA pseudouridine synthase A isoform X1 [Cucumis melo] >KAA0034150.1...	[more]
XP_011655540.1	2.5e-159	82.52	uncharacterized protein LOC101221274 isoform X1 [Cucumis sativus] >KGN51629.1 hy...	[more]
XP_023524389.1	3.3e-159	82.52	uncharacterized protein LOC111788293 [Cucurbita pepo subsp. pepo]	[more]
XP_038877866.1	9.7e-159	81.11	tRNA pseudouridine synthase A isoform X1 [Benincasa hispida]	[more]

BLAST of Sed0027668 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A9ILJ7	1.7e-44	36.90	tRNA pseudouridine synthase A OS=Bartonella tribocorum (strain CIP 105476 / IBS ...	[more]
B6IPI0	6.3e-44	39.45	tRNA pseudouridine synthase A OS=Rhodospirillum centenum (strain ATCC 51521 / SW...	[more]
A7IKI9	8.2e-44	38.68	tRNA pseudouridine synthase A OS=Xanthobacter autotrophicus (strain ATCC BAA-115...	[more]
Q6G547	1.1e-43	36.81	tRNA pseudouridine synthase A OS=Bartonella henselae (strain ATCC 49882 / DSM 28...	[more]
A7HPQ3	1.1e-43	37.85	tRNA pseudouridine synthase A OS=Parvibaculum lavamentivorans (strain DS-1 / DSM...	[more]

BLAST of Sed0027668 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3CZZ6	2.5e-160	83.09	tRNA pseudouridine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf...	[more]
A0A1S3BDG9	2.5e-160	83.09	tRNA pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103488841 PE=3 SV=1	[more]
A0A0A0KRX0	1.2e-159	82.52	tRNA pseudouridine synthase OS=Cucumis sativus OX=3659 GN=Csa_5G586020 PE=3 SV=1	[more]
A0A6J1KFZ8	6.1e-159	82.23	tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111492882 PE=3 SV=...	[more]
A0A6J1GY72	8.0e-159	82.23	tRNA pseudouridine synthase OS=Cucurbita moschata OX=3662 GN=LOC111458281 PE=3 S...	[more]

BLAST of Sed0027668 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G09800.1	1.4e-123	62.63	Pseudouridine synthase family protein	[more]
AT1G34150.1	2.2e-12	24.44	Pseudouridine synthase family protein	[more]
AT5G35400.1	7.0e-06	26.67	Pseudouridine synthase family protein	[more]
AT5G35400.2	7.0e-06	26.67	Pseudouridine synthase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR020097	Pseudouridine synthase I, TruA, alpha/beta domain	PFAM	PF01416	PseudoU_synth_1	coord: 196..279 e-value: 1.4E-11 score: 44.8 coord: 46..155 e-value: 5.0E-8 score: 33.4
IPR001406	Pseudouridine synthase I, TruA	PIRSF	PIRSF001430	PSU-A	coord: 40..280 e-value: 3.1E-54 score: 182.0
IPR001406	Pseudouridine synthase I, TruA	PANTHER	PTHR11142	PSEUDOURIDYLATE SYNTHASE	coord: 29..279
IPR001406	Pseudouridine synthase I, TruA	CDD	cd02570	PseudoU_synth_EcTruA	coord: 47..279 e-value: 5.16096E-79 score: 237.372
IPR020094	Pseudouridine synthase TruA/RsuA/RluB/E/F, N-terminal	GENE3D	3.30.70.580		coord: 38..157 e-value: 5.9E-27 score: 96.0
IPR020095	Pseudouridine synthase I, TruA, C-terminal	GENE3D	3.30.70.660		coord: 158..280 e-value: 2.7E-21 score: 77.8
None	No IPR available	PIRSR	PIRSR001430-2	PIRSR001430-2	coord: 80..229 e-value: 4.6E-36 score: 122.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..36
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..26
None	No IPR available	PANTHER	PTHR11142:SF0	TRNA PSEUDOURIDINE SYNTHASE-LIKE 1	coord: 29..279
IPR020103	Pseudouridine synthase, catalytic domain superfamily	SUPERFAMILY	55120	Pseudouridine synthase	coord: 40..279

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sed0027668.1	Sed0027668.1	mRNA
Sed0027668.2	Sed0027668.2	mRNA
Sed0027668.3	Sed0027668.3	mRNA
Sed0027668.4	Sed0027668.4	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0031119	tRNA pseudouridine synthesis
biological_process	GO:0001522	pseudouridine synthesis
biological_process	GO:0009451	RNA modification
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0106029	tRNA pseudouridine synthase activity
molecular_function	GO:0009982	pseudouridine synthase activity