Sed0027618 (gene) Chayote v1

BLAST of Sed0027618 vs. NCBI nr
Match: XP_038895748.1 (uncharacterized protein LOC120083912 [Benincasa hispida])

HSP 1 Score: 806.2 bits (2081), Expect = 1.8e-229
Identity = 410/565 (72.57%), Postives = 480/565 (84.96%), Query Frame = 0

Query: 7   SQVWGFIKQVCAGRWFSVFASLLMMMGTGTTYIYGTYSKVIKTQFDYTQTQINTLGFAKD 66
           S+ W F KQV AGRWFSVFA L+MM+G G+TYIYGTYSKVIKTQFDY+QTQ+N LGFAKD
Sbjct: 120 SKAWSFTKQVVAGRWFSVFAGLMMMLGNGSTYIYGTYSKVIKTQFDYSQTQVNILGFAKD 179

Query: 67  LGSNLGIFAGLLAEVAPSSVLFFISAFLNFSCFFMIWLSITHRIARPKFWQIFLYICVGT 126
           LGSN+GIFAGLL EVAP+ VLF I +FLNF+ FFMIWL+ITHRI +PKFWQ+F  +C GT
Sbjct: 180 LGSNVGIFAGLLGEVAPAWVLFLIGSFLNFTGFFMIWLAITHRIPQPKFWQMFCLVCFGT 239

Query: 127 NSSNFANTAILVNGVRNFPDRRGVILGLLKGFVGIGGAVFTQLYLAFYGNDDPSNQVLLF 186
           NSSNFANTAI+V+ V NFPDRRG+ILGLLKGFVGIGGAV+TQ+YL  YG+DDP+N VLLF
Sbjct: 240 NSSNFANTAIMVSSVGNFPDRRGIILGLLKGFVGIGGAVYTQIYLGIYGHDDPANLVLLF 299

Query: 187 SWLPSVLILILFSSIRPINSRKHPEELKVFYHLLYISILLALFILFLTIAQKQTSFSHGG 246
           +WLPS + L+LF+SIRPI  RKHPEELKVFYHLLY+SI++ALFILFLT+AQK+  FS  G
Sbjct: 300 AWLPSTVTLLLFTSIRPIRIRKHPEELKVFYHLLYVSIIIALFILFLTLAQKEVVFSRSG 359

Query: 247 YVSGACVIIATLCLPLLIAFREEFFLYKLNQNPHPNSSSSVVVSIIPEQQQAASKEITTL 306
           Y SGA VI+A L LPLLIA REE+ LYKLN+    +  +SV +S I EQ+Q      T +
Sbjct: 360 YGSGATVIVALLFLPLLIACREEYLLYKLNR----DDDASVTLS-INEQKQPPPVTTTEI 419

Query: 307 PTNS-----NKPARGDDFTILQAMFSKDMTLICLATFSGCGSSLAAIDNLGQIGESLHYP 366
             +      NKP RG+DF+ILQA+FS DM LI LATF+G GSSLAAIDNLGQ+GESL YP
Sbjct: 420 SQSCCSGIWNKPERGEDFSILQAIFSIDMALIYLATFAGSGSSLAAIDNLGQVGESLGYP 479

Query: 367 PQAISIFVSWVSIFSFFGRVCSGFISETLMVKYKLPRPLIFSFAFLITCIGQLCIAFPSS 426
           P+AISIFVSWVSIF+FFGRV SGF+SET+M+KYKLPRP++F+ AFLITCIGQL IA+P+ 
Sbjct: 480 PRAISIFVSWVSIFNFFGRVFSGFVSETMMIKYKLPRPVMFAVAFLITCIGQLFIAYPTP 539

Query: 427 GSVYAASLIIGFGFGSQVPLLFAIISELFGLKHYSTLFNWGQLIVPLGSYILNVEVVGKL 486
           GSVY AS+IIGFGFG QVPLLFAIISELFGLKHYSTLFN+GQL+VPLGSY+LNV+V G+L
Sbjct: 540 GSVYLASMIIGFGFGFQVPLLFAIISELFGLKHYSTLFNFGQLVVPLGSYLLNVDVTGRL 599

Query: 487 YDIEASKEHLRAGGIVGKGLTCNGAHCFSGSFTILASFTLFGALSMLVLAYRTREFYRGD 546
           YD EA +E  +   I GKG+TC GAHCFSGSFTILA  TLFGA++MLVLAYRTR+FYRGD
Sbjct: 600 YDKEALREGKK---ITGKGITCTGAHCFSGSFTILAGATLFGAMTMLVLAYRTRQFYRGD 659

Query: 547 VYNKYKEDLWIQQSEMEFYHLEKKK 567
           VY KY+ED+WI Q+EMEFYHL+KKK
Sbjct: 660 VYKKYREDIWIPQTEMEFYHLDKKK 676

BLAST of Sed0027618 vs. NCBI nr
Match: XP_022970240.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima])

HSP 1 Score: 795.8 bits (2054), Expect = 2.4e-226
Identity = 407/574 (70.91%), Postives = 473/574 (82.40%), Query Frame = 0

Query: 1   MGWEEGSQVWGFIKQVCAGRWFSVFASLLMMMGTGTTYIYGTYSKVIKTQFDYTQTQINT 60
           MG + GS+ WGFIKQV  GRWFSVFA L+MM+G GTTY++ TYSKVIKTQFDY+QTQINT
Sbjct: 1   MGNKGGSEAWGFIKQVVTGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT 60

Query: 61  LGFAKDLGSNLGIFAGLLAEVAPSSVLFFISAFLNFSCFFMIWLSITHRIARPKFWQIFL 120
           LGFAKDLGSN+GI AGLLAEV P+ VLF + AF NF+ FF+IWLSIT RIA+PKFWQ+F+
Sbjct: 61  LGFAKDLGSNVGIIAGLLAEVTPTWVLFIVGAFQNFTGFFLIWLSITARIAKPKFWQMFI 120

Query: 121 YICVGTNSSNFANTAILVNGVRNFPDRRGVILGLLKGFVGIGGAVFTQLYLAFYGNDDPS 180
            +C GTNSSN+ANTAI+V  VRNFPDRRG+ILGLLKG+VGIGGA+ +QLYLA YG++DPS
Sbjct: 121 LVCFGTNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIMSQLYLAIYGHNDPS 180

Query: 181 NQVLLFSWLPSVLILILFSSIRPINSRKHPEELKVFYHLLYISILLALFILFLTIAQKQT 240
           N VLLF+WLPS+LILILF SIRPI  RKHP ELKVFY LLY+SILLA+FILFLTIAQKQ 
Sbjct: 181 NLVLLFAWLPSLLILILFPSIRPIRIRKHPNELKVFYQLLYVSILLAIFILFLTIAQKQV 240

Query: 241 SFSHGGYVSGACVIIATLCLPLLIAFREEFFLYKLN-QNPHPNSSSSVVVSIIPEQQQAA 300
            FS  GYV GA  +++ LC+P+LIA REE  LYKLN Q   P+ + S+V    P    +A
Sbjct: 241 VFSQAGYVGGASFVVSLLCVPILIACREELLLYKLNKQTAGPSVTVSMVHQNPPTLSASA 300

Query: 301 SKEITTLPTNS-----NKPARGDDFTILQAMFSKDMTLICLATFSGCGSSLAAIDNLGQI 360
             EI  +  +      NKP RG+DFTILQA+FSKDM LICLATFSGCGSSLAAIDNLGQ+
Sbjct: 301 DSEIPEILPSCCENIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNLGQV 360

Query: 361 GESLHYPPQAISIFVSWVSIFSFFGRVCSGFISETLMVKYKLPRPLIFSFAFLITCIGQL 420
           GESL YP +AI I VSWVSIF+FFGRV SGFISETLM KYKLPRPL FSFAFLITC+GQL
Sbjct: 361 GESLGYPQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFSFAFLITCVGQL 420

Query: 421 CIAFPSSGSVYAASLIIGFGFGSQVPLLFAIISELFGLKHYSTLFNWGQLIVPLGSYILN 480
            IA+P  GSVY A++IIGFGFG+Q P+LFA+ISE+FGL+HYSTLFN GQL VPLGSYILN
Sbjct: 421 FIAYPFPGSVYFAAIIIGFGFGAQNPMLFAVISEMFGLRHYSTLFNCGQLTVPLGSYILN 480

Query: 481 VEVVGKLYDIEASKEHLRAGGIVGKGLTCNGAHCFSGSFTILASFTLFGALSMLVLAYRT 540
           V+VVGKLYD+EA  E  +   I GKGLTC GAHCF GSFTILA+ TLFGA+ MLVLAYRT
Sbjct: 481 VDVVGKLYDMEALHEGKQ---IAGKGLTCTGAHCFGGSFTILAAATLFGAVVMLVLAYRT 540

Query: 541 REFYRGDVYNKYKEDLWIQQSEMEFYHLEKKKKV 569
           REFY+ DVY  + E++WI Q++MEFY L+ KK +
Sbjct: 541 REFYKRDVYKNFNEEIWIPQTQMEFYRLDNKKSI 571

BLAST of Sed0027618 vs. NCBI nr
Match: XP_022970365.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima] >XP_022970399.1 protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima])

HSP 1 Score: 794.7 bits (2051), Expect = 5.4e-226
Identity = 407/574 (70.91%), Postives = 472/574 (82.23%), Query Frame = 0

Query: 1   MGWEEGSQVWGFIKQVCAGRWFSVFASLLMMMGTGTTYIYGTYSKVIKTQFDYTQTQINT 60
           MG + GS+ WGFIKQV AGRWFSVFA L+MM+G GTTY++ TYSKVIKTQFDY+QTQINT
Sbjct: 1   MGNKGGSEAWGFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT 60

Query: 61  LGFAKDLGSNLGIFAGLLAEVAPSSVLFFISAFLNFSCFFMIWLSITHRIARPKFWQIFL 120
           LGFAKDLGSN+GI AGLLAEV P+ VLF I AF NF  FF+IWLSIT RIA+PKFWQ+F+
Sbjct: 61  LGFAKDLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFIGFFLIWLSITARIAKPKFWQMFI 120

Query: 121 YICVGTNSSNFANTAILVNGVRNFPDRRGVILGLLKGFVGIGGAVFTQLYLAFYGNDDPS 180
            +C GTNSSN+ANTAI+V  VRNFPDRRG+ILGLLKG+VGIGGA+ +Q YLA YG++DPS
Sbjct: 121 LVCFGTNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIMSQFYLAIYGHNDPS 180

Query: 181 NQVLLFSWLPSVLILILFSSIRPINSRKHPEELKVFYHLLYISILLALFILFLTIAQKQT 240
           N VLLF+WLPS+LILILF SIRPI  RKHP ELKVFY LLY+SILLA+FILFLTIAQKQ 
Sbjct: 181 NLVLLFAWLPSLLILILFPSIRPIRIRKHPNELKVFYQLLYVSILLAIFILFLTIAQKQV 240

Query: 241 SFSHGGYVSGACVIIATLCLPLLIAFREEFFLYKLN-QNPHPNSSSSVVVSIIPEQQQAA 300
            FS  GYV GA  +++ LC+P+LIA REE  LYKLN Q   P+ + S+V    P    +A
Sbjct: 241 VFSQAGYVGGASFVVSLLCVPILIACREELLLYKLNKQTAGPSVTVSMVHQNPPTLPASA 300

Query: 301 SKEITTLPTNS-----NKPARGDDFTILQAMFSKDMTLICLATFSGCGSSLAAIDNLGQI 360
             EI  +  +      NKP RG+DFTILQA+FSKDM LICLATFSGCGSSLAAIDNLGQ+
Sbjct: 301 DSEIPEISPSCCENIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNLGQV 360

Query: 361 GESLHYPPQAISIFVSWVSIFSFFGRVCSGFISETLMVKYKLPRPLIFSFAFLITCIGQL 420
           GESL YP +AI I VSWVSIF+FFGRV SGFISETLM KYKLPRPL FSFAFLITC+GQL
Sbjct: 361 GESLGYPQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFSFAFLITCVGQL 420

Query: 421 CIAFPSSGSVYAASLIIGFGFGSQVPLLFAIISELFGLKHYSTLFNWGQLIVPLGSYILN 480
            IA+P  GSVY A++IIGFGFG+Q P+LFA+ISE+FGL+ YSTLFN GQL VPLGSYILN
Sbjct: 421 FIAYPFPGSVYLAAIIIGFGFGAQNPMLFAVISEMFGLRRYSTLFNCGQLAVPLGSYILN 480

Query: 481 VEVVGKLYDIEASKEHLRAGGIVGKGLTCNGAHCFSGSFTILASFTLFGALSMLVLAYRT 540
           V+VVGKLYD+EA +E  +   I GKGLTC GAHCF GSFTILA+ TLFGA+ MLVLAYRT
Sbjct: 481 VDVVGKLYDMEALREGKK---IAGKGLTCTGAHCFGGSFTILAAATLFGAVVMLVLAYRT 540

Query: 541 REFYRGDVYNKYKEDLWIQQSEMEFYHLEKKKKV 569
           REFY+ DVY  + E++WI Q++MEFY L+ KK +
Sbjct: 541 REFYKRDVYKNFNEEIWIPQTQMEFYRLDSKKSI 571

BLAST of Sed0027618 vs. NCBI nr
Match: XP_023520297.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 793.1 bits (2047), Expect = 1.6e-225
Identity = 404/574 (70.38%), Postives = 471/574 (82.06%), Query Frame = 0

Query: 1   MGWEEGSQVWGFIKQVCAGRWFSVFASLLMMMGTGTTYIYGTYSKVIKTQFDYTQTQINT 60
           MG + GS+ W FIKQV AGRWFSVFA L+MM+G GTTY++ TYSKVIKTQFDY+QTQINT
Sbjct: 1   MGNKGGSEAWAFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT 60

Query: 61  LGFAKDLGSNLGIFAGLLAEVAPSSVLFFISAFLNFSCFFMIWLSITHRIARPKFWQIFL 120
           LGFAKDLGSN+GI AGLLAEV P+ VLF I AF NF+ FF+IW+SIT RIA+PKFWQ+F+
Sbjct: 61  LGFAKDLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFTGFFLIWVSITARIAKPKFWQMFI 120

Query: 121 YICVGTNSSNFANTAILVNGVRNFPDRRGVILGLLKGFVGIGGAVFTQLYLAFYGNDDPS 180
            +C  TNSSN+ANTAI+V  VRNFPDRRG+ILGLLKG+VGIGGA+ +QLYLA YG++DPS
Sbjct: 121 LVCFSTNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIISQLYLAIYGHNDPS 180

Query: 181 NQVLLFSWLPSVLILILFSSIRPINSRKHPEELKVFYHLLYISILLALFILFLTIAQKQT 240
           N V LF+WLPS+LILILF SIRPI  RKHP+ELKVFY LLY+SILLA+FILFLTIAQKQ 
Sbjct: 181 NLVFLFAWLPSLLILILFPSIRPIRIRKHPDELKVFYQLLYVSILLAIFILFLTIAQKQV 240

Query: 241 SFSHGGYVSGACVIIATLCLPLLIAFREEFFLYKLN-QNPHPNSSSSVVVSIIPEQQQAA 300
            FS  GYV GA  ++  LC+P+LIA REE  LYKLN Q   P+   S++    P    +A
Sbjct: 241 VFSQAGYVGGASFVVCLLCVPILIACREELLLYKLNKQTAGPSIPVSMLHQNPPTLATSA 300

Query: 301 SKEITTLPTNS-----NKPARGDDFTILQAMFSKDMTLICLATFSGCGSSLAAIDNLGQI 360
           S E+  +  +      NKP RG+DFTILQA+FSKDM LICLATFSGCGSSLAAIDNLGQ+
Sbjct: 301 SSEVPEVSPSCCQNIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNLGQV 360

Query: 361 GESLHYPPQAISIFVSWVSIFSFFGRVCSGFISETLMVKYKLPRPLIFSFAFLITCIGQL 420
           GESL YP +AI I VSWVSIF+FFGRV SGFISETLM KYKLPRPL F+FAFLITCIGQL
Sbjct: 361 GESLGYPQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFAFAFLITCIGQL 420

Query: 421 CIAFPSSGSVYAASLIIGFGFGSQVPLLFAIISELFGLKHYSTLFNWGQLIVPLGSYILN 480
            IA+P  GSVY A++IIGFGFG+Q P+LFA+ISE+FGLK YSTLFN GQL  PLGSYILN
Sbjct: 421 FIAYPFPGSVYLAAIIIGFGFGAQNPMLFAVISEMFGLKRYSTLFNCGQLAAPLGSYILN 480

Query: 481 VEVVGKLYDIEASKEHLRAGGIVGKGLTCNGAHCFSGSFTILASFTLFGALSMLVLAYRT 540
           V+VVGKLYD+EA +EH +   I G+GLTC GAHCF GSFTILA+ TLFGAL M VLAYRT
Sbjct: 481 VDVVGKLYDMEALREHKQ---IAGRGLTCTGAHCFGGSFTILAAATLFGALVMFVLAYRT 540

Query: 541 REFYRGDVYNKYKEDLWIQQSEMEFYHLEKKKKV 569
           REFY+ DVY  + E++WI Q++MEFYHL+ KK +
Sbjct: 541 REFYKRDVYKNFNEEIWIPQTQMEFYHLDNKKSI 571

BLAST of Sed0027618 vs. NCBI nr
Match: KAG6583526.1 (Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia] >KAG6583533.1 Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia] >KAG6583538.1 Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia] >KAG6583544.1 Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 788.5 bits (2035), Expect = 3.9e-224
Identity = 402/574 (70.03%), Postives = 470/574 (81.88%), Query Frame = 0

Query: 1   MGWEEGSQVWGFIKQVCAGRWFSVFASLLMMMGTGTTYIYGTYSKVIKTQFDYTQTQINT 60
           MG + GS+ W FIKQV AGRWFSVFA L+MM+G GTTY++ TYSKVIKTQFDY+QTQINT
Sbjct: 1   MGNKGGSEAWAFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT 60

Query: 61  LGFAKDLGSNLGIFAGLLAEVAPSSVLFFISAFLNFSCFFMIWLSITHRIARPKFWQIFL 120
           LGFAKDLGSN+GI AGLLAEV P+ VLF I AF NF+ FF+IWLSIT RIA+PKFWQ+F+
Sbjct: 61  LGFAKDLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFTGFFLIWLSITARIAKPKFWQMFI 120

Query: 121 YICVGTNSSNFANTAILVNGVRNFPDRRGVILGLLKGFVGIGGAVFTQLYLAFYGNDDPS 180
            +C+GTNSSN+ANTAI+V  VRNFPDRRG+ILGLLKG+VGIGGA+ +QLYLA YG++DPS
Sbjct: 121 LVCLGTNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIISQLYLAIYGHNDPS 180

Query: 181 NQVLLFSWLPSVLILILFSSIRPINSRKHPEELKVFYHLLYISILLALFILFLTIAQKQT 240
           N VLLF+WLPS+LILILF SIRPI  RKHP+ELKVFY LLY+SILLA+FILFLTIAQKQ 
Sbjct: 181 NLVLLFAWLPSLLILILFPSIRPIRIRKHPDELKVFYQLLYVSILLAIFILFLTIAQKQV 240

Query: 241 SFSHGGYVSGACVIIATLCLPLLIAFREEFFLYKLN-QNPHPNSSSSVVVSIIPEQQQAA 300
            FS  GYV GA  ++  LC+P+LIA REE  LYKLN Q   P+ + S++    P    +A
Sbjct: 241 VFSQAGYVGGASFVVCLLCVPILIACREELLLYKLNKQTAAPSVALSMLHQNPPTLPTSA 300

Query: 301 SKEITTL-----PTNSNKPARGDDFTILQAMFSKDMTLICLATFSGCGSSLAAIDNLGQI 360
             EI  +     P   NKP RG+DFTILQA+FSKDM LICLATFSGCGSSLAAIDNLGQ+
Sbjct: 301 DSEIPEISPSCCPNIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNLGQV 360

Query: 361 GESLHYPPQAISIFVSWVSIFSFFGRVCSGFISETLMVKYKLPRPLIFSFAFLITCIGQL 420
           GESL Y  +AI I VSWVSIF+FFGRV SGFISETLM KYKLPRPL F+FAFLITC+GQL
Sbjct: 361 GESLGYSQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFAFAFLITCVGQL 420

Query: 421 CIAFPSSGSVYAASLIIGFGFGSQVPLLFAIISELFGLKHYSTLFNWGQLIVPLGSYILN 480
            IA+P  GS+Y A++IIGFGFG+Q P+LFA+ISE+FGLK YSTLFN GQL  P GSYILN
Sbjct: 421 FIAYPFPGSLYLAAIIIGFGFGAQNPMLFAVISEMFGLKRYSTLFNCGQLAAPFGSYILN 480

Query: 481 VEVVGKLYDIEASKEHLRAGGIVGKGLTCNGAHCFSGSFTILASFTLFGALSMLVLAYRT 540
           V+VVGKLYD+EA +E  +   I GKGLTC GAHCF GSFT+LA+ TLFGA+ M VLAYRT
Sbjct: 481 VDVVGKLYDMEALRERKQ---IAGKGLTCTGAHCFGGSFTVLAAATLFGAVVMFVLAYRT 540

Query: 541 REFYRGDVYNKYKEDLWIQQSEMEFYHLEKKKKV 569
           REFY+ DVY  + E++WI Q+EMEFY L+ KK +
Sbjct: 541 REFYKRDVYKNFNEEIWIPQTEMEFYRLDNKKNI 571

BLAST of Sed0027618 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 2.0e-34
Identity = 146/540 (27.04%), Postives = 247/540 (45.74%), Query Frame = 0

Query: 20  RWFSVFASLLMMMGTGTTYIYGTYSKVIKTQFDYTQTQINTLGFAKDLGSNLGIFAGLLA 79
           +W  + A++ +   TGT + +  YS  +K+    +Q ++N L  A DLG   G  +G+  
Sbjct: 43  KWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSGIAL 102

Query: 80  EVAPSSVLFFISAFLNFSCFFMIWLSITHRIARPKFWQIFLYICVGTNSSNFANTAILVN 139
              P SV+ F +A + F  + + WL IT+ I  P +  +FL   +   S  + NTA  + 
Sbjct: 103 GYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLP-YSLVFLCCLLAGLSICWFNTACFIL 162

Query: 140 GVRNFPDRRGVILGLLKGFVGIGGAVFTQLYLAFYGNDDPSNQVLLFSWLPSVLILILFS 199
            +R+FP+ R + L L   F GI  A+++  + A   N   SN  LL + L  V +++ F+
Sbjct: 163 CIRHFPNNRALALSLTVSFNGISAALYSLAFNAI--NPSSSNLYLLLNSL--VPLVVSFA 222

Query: 200 SIRPI------------NSRKHPEELKVFYHLLYISILLALFILF-LTIAQKQTSFSHGG 259
           ++ P+            +SR+H        H+  I  +LA+   F L ++   TS +   
Sbjct: 223 ALYPVLTKPSLDTTPDYDSRRHDS------HVFTILNVLAVITSFHLLLSSSSTSSARLN 282

Query: 260 YVSGACVIIATLCLPLLIAFREEFFLYKLNQNPHPNSSSSVVVSIIPEQQQAASKEITT- 319
           ++    +++  LC PLL+ +  ++FL  +N   +  SS  V+++I   + Q  S    T 
Sbjct: 283 FIGAVVLLVFPLCAPLLV-YARDYFLPVINARLNHESSGYVMLNIDELKNQKTSVSSKTG 342

Query: 320 -----LPTNSNKPARGDDFTILQAMFSKDMTLICLATFSGCGSSLAAIDNLGQIGESLHY 379
                     N    GD+ +    +   +  L  +A F G    L   +NLGQI +SL  
Sbjct: 343 YEHMGTAKEGNTVRLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSL-- 402

Query: 380 PPQAISIFVSWVSIFSFFGRVCSGFISETLMVKYKLPRPLIFSFAFLITCIGQLCIAFPS 439
             Q  +  V+  S FSFFGR+ S    + +  +++L R   F+ A L T I    +A  S
Sbjct: 403 -GQNSTTLVTIYSSFSFFGRLLSA-APDFMHKRFRLTRTGWFAIALLPTPIAFFLLAVSS 462

Query: 440 S--GSVYAASLIIGFGFGSQVPLLFAIISELFGLKHYSTLFNWGQLIVPLGSYILNVEVV 499
           S   ++  A+ +IG   G       +I S+LFG        N     +P+GS +L   + 
Sbjct: 463 SQQTALQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGS-LLYGYIA 522

Query: 500 GKLYDIEASKEHLRAGGIVGKGLTCNGAHCFSGSFTILASFTLFGALSMLVLAYRTREFY 539
             +Y+  AS +      IV   + C G  C+  +F      ++ G +S L L  RT+  Y
Sbjct: 523 ASIYEANASPD---ITPIVSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTKPVY 562

BLAST of Sed0027618 vs. ExPASy TrEMBL
Match: A0A6J1I2B3 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111469248 PE=4 SV=1)

HSP 1 Score: 795.8 bits (2054), Expect = 1.2e-226
Identity = 407/574 (70.91%), Postives = 473/574 (82.40%), Query Frame = 0

Query: 1   MGWEEGSQVWGFIKQVCAGRWFSVFASLLMMMGTGTTYIYGTYSKVIKTQFDYTQTQINT 60
           MG + GS+ WGFIKQV  GRWFSVFA L+MM+G GTTY++ TYSKVIKTQFDY+QTQINT
Sbjct: 1   MGNKGGSEAWGFIKQVVTGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT 60

Query: 61  LGFAKDLGSNLGIFAGLLAEVAPSSVLFFISAFLNFSCFFMIWLSITHRIARPKFWQIFL 120
           LGFAKDLGSN+GI AGLLAEV P+ VLF + AF NF+ FF+IWLSIT RIA+PKFWQ+F+
Sbjct: 61  LGFAKDLGSNVGIIAGLLAEVTPTWVLFIVGAFQNFTGFFLIWLSITARIAKPKFWQMFI 120

Query: 121 YICVGTNSSNFANTAILVNGVRNFPDRRGVILGLLKGFVGIGGAVFTQLYLAFYGNDDPS 180
            +C GTNSSN+ANTAI+V  VRNFPDRRG+ILGLLKG+VGIGGA+ +QLYLA YG++DPS
Sbjct: 121 LVCFGTNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIMSQLYLAIYGHNDPS 180

Query: 181 NQVLLFSWLPSVLILILFSSIRPINSRKHPEELKVFYHLLYISILLALFILFLTIAQKQT 240
           N VLLF+WLPS+LILILF SIRPI  RKHP ELKVFY LLY+SILLA+FILFLTIAQKQ 
Sbjct: 181 NLVLLFAWLPSLLILILFPSIRPIRIRKHPNELKVFYQLLYVSILLAIFILFLTIAQKQV 240

Query: 241 SFSHGGYVSGACVIIATLCLPLLIAFREEFFLYKLN-QNPHPNSSSSVVVSIIPEQQQAA 300
            FS  GYV GA  +++ LC+P+LIA REE  LYKLN Q   P+ + S+V    P    +A
Sbjct: 241 VFSQAGYVGGASFVVSLLCVPILIACREELLLYKLNKQTAGPSVTVSMVHQNPPTLSASA 300

Query: 301 SKEITTLPTNS-----NKPARGDDFTILQAMFSKDMTLICLATFSGCGSSLAAIDNLGQI 360
             EI  +  +      NKP RG+DFTILQA+FSKDM LICLATFSGCGSSLAAIDNLGQ+
Sbjct: 301 DSEIPEILPSCCENIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNLGQV 360

Query: 361 GESLHYPPQAISIFVSWVSIFSFFGRVCSGFISETLMVKYKLPRPLIFSFAFLITCIGQL 420
           GESL YP +AI I VSWVSIF+FFGRV SGFISETLM KYKLPRPL FSFAFLITC+GQL
Sbjct: 361 GESLGYPQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFSFAFLITCVGQL 420

Query: 421 CIAFPSSGSVYAASLIIGFGFGSQVPLLFAIISELFGLKHYSTLFNWGQLIVPLGSYILN 480
            IA+P  GSVY A++IIGFGFG+Q P+LFA+ISE+FGL+HYSTLFN GQL VPLGSYILN
Sbjct: 421 FIAYPFPGSVYFAAIIIGFGFGAQNPMLFAVISEMFGLRHYSTLFNCGQLTVPLGSYILN 480

Query: 481 VEVVGKLYDIEASKEHLRAGGIVGKGLTCNGAHCFSGSFTILASFTLFGALSMLVLAYRT 540
           V+VVGKLYD+EA  E  +   I GKGLTC GAHCF GSFTILA+ TLFGA+ MLVLAYRT
Sbjct: 481 VDVVGKLYDMEALHEGKQ---IAGKGLTCTGAHCFGGSFTILAAATLFGAVVMLVLAYRT 540

Query: 541 REFYRGDVYNKYKEDLWIQQSEMEFYHLEKKKKV 569
           REFY+ DVY  + E++WI Q++MEFY L+ KK +
Sbjct: 541 REFYKRDVYKNFNEEIWIPQTQMEFYRLDNKKSI 571

BLAST of Sed0027618 vs. ExPASy TrEMBL
Match: A0A6J1I5D7 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111469398 PE=4 SV=1)

HSP 1 Score: 794.7 bits (2051), Expect = 2.6e-226
Identity = 407/574 (70.91%), Postives = 472/574 (82.23%), Query Frame = 0

Query: 1   MGWEEGSQVWGFIKQVCAGRWFSVFASLLMMMGTGTTYIYGTYSKVIKTQFDYTQTQINT 60
           MG + GS+ WGFIKQV AGRWFSVFA L+MM+G GTTY++ TYSKVIKTQFDY+QTQINT
Sbjct: 1   MGNKGGSEAWGFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT 60

Query: 61  LGFAKDLGSNLGIFAGLLAEVAPSSVLFFISAFLNFSCFFMIWLSITHRIARPKFWQIFL 120
           LGFAKDLGSN+GI AGLLAEV P+ VLF I AF NF  FF+IWLSIT RIA+PKFWQ+F+
Sbjct: 61  LGFAKDLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFIGFFLIWLSITARIAKPKFWQMFI 120

Query: 121 YICVGTNSSNFANTAILVNGVRNFPDRRGVILGLLKGFVGIGGAVFTQLYLAFYGNDDPS 180
            +C GTNSSN+ANTAI+V  VRNFPDRRG+ILGLLKG+VGIGGA+ +Q YLA YG++DPS
Sbjct: 121 LVCFGTNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIMSQFYLAIYGHNDPS 180

Query: 181 NQVLLFSWLPSVLILILFSSIRPINSRKHPEELKVFYHLLYISILLALFILFLTIAQKQT 240
           N VLLF+WLPS+LILILF SIRPI  RKHP ELKVFY LLY+SILLA+FILFLTIAQKQ 
Sbjct: 181 NLVLLFAWLPSLLILILFPSIRPIRIRKHPNELKVFYQLLYVSILLAIFILFLTIAQKQV 240

Query: 241 SFSHGGYVSGACVIIATLCLPLLIAFREEFFLYKLN-QNPHPNSSSSVVVSIIPEQQQAA 300
            FS  GYV GA  +++ LC+P+LIA REE  LYKLN Q   P+ + S+V    P    +A
Sbjct: 241 VFSQAGYVGGASFVVSLLCVPILIACREELLLYKLNKQTAGPSVTVSMVHQNPPTLPASA 300

Query: 301 SKEITTLPTNS-----NKPARGDDFTILQAMFSKDMTLICLATFSGCGSSLAAIDNLGQI 360
             EI  +  +      NKP RG+DFTILQA+FSKDM LICLATFSGCGSSLAAIDNLGQ+
Sbjct: 301 DSEIPEISPSCCENIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNLGQV 360

Query: 361 GESLHYPPQAISIFVSWVSIFSFFGRVCSGFISETLMVKYKLPRPLIFSFAFLITCIGQL 420
           GESL YP +AI I VSWVSIF+FFGRV SGFISETLM KYKLPRPL FSFAFLITC+GQL
Sbjct: 361 GESLGYPQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFSFAFLITCVGQL 420

Query: 421 CIAFPSSGSVYAASLIIGFGFGSQVPLLFAIISELFGLKHYSTLFNWGQLIVPLGSYILN 480
            IA+P  GSVY A++IIGFGFG+Q P+LFA+ISE+FGL+ YSTLFN GQL VPLGSYILN
Sbjct: 421 FIAYPFPGSVYLAAIIIGFGFGAQNPMLFAVISEMFGLRRYSTLFNCGQLAVPLGSYILN 480

Query: 481 VEVVGKLYDIEASKEHLRAGGIVGKGLTCNGAHCFSGSFTILASFTLFGALSMLVLAYRT 540
           V+VVGKLYD+EA +E  +   I GKGLTC GAHCF GSFTILA+ TLFGA+ MLVLAYRT
Sbjct: 481 VDVVGKLYDMEALREGKK---IAGKGLTCTGAHCFGGSFTILAAATLFGAVVMLVLAYRT 540

Query: 541 REFYRGDVYNKYKEDLWIQQSEMEFYHLEKKKKV 569
           REFY+ DVY  + E++WI Q++MEFY L+ KK +
Sbjct: 541 REFYKRDVYKNFNEEIWIPQTQMEFYRLDSKKSI 571

BLAST of Sed0027618 vs. ExPASy TrEMBL
Match: A0A6J1HLT1 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111464780 PE=4 SV=1)

HSP 1 Score: 786.6 bits (2030), Expect = 7.1e-224
Identity = 401/574 (69.86%), Postives = 469/574 (81.71%), Query Frame = 0

Query: 1   MGWEEGSQVWGFIKQVCAGRWFSVFASLLMMMGTGTTYIYGTYSKVIKTQFDYTQTQINT 60
           MG + GS+ W FIKQV AGRWFSVFA L+MM+G GTTY++ TYSKVIKTQFDY+QTQINT
Sbjct: 1   MGNKGGSEAWAFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT 60

Query: 61  LGFAKDLGSNLGIFAGLLAEVAPSSVLFFISAFLNFSCFFMIWLSITHRIARPKFWQIFL 120
           LGFAKDLGSN+GI AGLLAEV P+ VLF I AF NF+ FF+IWLSIT RIA+PKFWQ+F+
Sbjct: 61  LGFAKDLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFTGFFLIWLSITARIAKPKFWQMFI 120

Query: 121 YICVGTNSSNFANTAILVNGVRNFPDRRGVILGLLKGFVGIGGAVFTQLYLAFYGNDDPS 180
            +C GTNSSN+ANTAI+V  VRNFPDRRG+ILGLLKG+VGIGGA+ +QLYLA YG++DPS
Sbjct: 121 LVCFGTNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIISQLYLAIYGHNDPS 180

Query: 181 NQVLLFSWLPSVLILILFSSIRPINSRKHPEELKVFYHLLYISILLALFILFLTIAQKQT 240
           N VLLF+WLPS+LILILF SIRPI  RKHP+ELKVFY LLY+SILLA+FILFLTIAQKQ 
Sbjct: 181 NLVLLFAWLPSLLILILFPSIRPIRIRKHPDELKVFYQLLYVSILLAIFILFLTIAQKQV 240

Query: 241 SFSHGGYVSGACVIIATLCLPLLIAFREEFFLYKLN-QNPHPNSSSSVVVSIIPEQQQAA 300
            FS  GYV GA  ++  LC+P+LIA REE  LYKLN +   P+ + S++    P    +A
Sbjct: 241 VFSQAGYVGGASFVVCLLCVPILIACREELLLYKLNKETAAPSVALSMLHQNPPTLPTSA 300

Query: 301 SKEITTL-----PTNSNKPARGDDFTILQAMFSKDMTLICLATFSGCGSSLAAIDNLGQI 360
             EI  +     P   NKP RG+DFTILQA+FSKDM LICLATFSGCGSSLAAIDNLGQ+
Sbjct: 301 DSEIPEISPSCCPNIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNLGQV 360

Query: 361 GESLHYPPQAISIFVSWVSIFSFFGRVCSGFISETLMVKYKLPRPLIFSFAFLITCIGQL 420
           GESL Y  +AI I VSWVSIF+FFGRV SGFISETLM KYKLPRPL F+FAFLITC+GQL
Sbjct: 361 GESLGYSQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFAFAFLITCVGQL 420

Query: 421 CIAFPSSGSVYAASLIIGFGFGSQVPLLFAIISELFGLKHYSTLFNWGQLIVPLGSYILN 480
            IA+P  GS+Y A++IIGFGFG+Q P+LFA+ISE+FGLK YSTLFN GQL  P GSYILN
Sbjct: 421 FIAYPFPGSLYLAAIIIGFGFGAQNPMLFAVISEMFGLKRYSTLFNCGQLAAPFGSYILN 480

Query: 481 VEVVGKLYDIEASKEHLRAGGIVGKGLTCNGAHCFSGSFTILASFTLFGALSMLVLAYRT 540
           V+VVGKLYD+EA +E  +   I GKGLTC GAHCF GSFT+LA+ TLFGA+ M VLAYRT
Sbjct: 481 VDVVGKLYDMEALRERKQ---IAGKGLTCTGAHCFGGSFTVLAAATLFGAVVMFVLAYRT 540

Query: 541 REFYRGDVYNKYKEDLWIQQSEMEFYHLEKKKKV 569
           REFY+ DVY  + E++WI Q+EMEFY L+ KK +
Sbjct: 541 REFYKRDVYKNFNEEIWIPQTEMEFYRLDNKKNI 571

BLAST of Sed0027618 vs. ExPASy TrEMBL
Match: A0A6J1HP70 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111464775 PE=4 SV=1)

HSP 1 Score: 785.4 bits (2027), Expect = 1.6e-223
Identity = 401/574 (69.86%), Postives = 468/574 (81.53%), Query Frame = 0

Query: 1   MGWEEGSQVWGFIKQVCAGRWFSVFASLLMMMGTGTTYIYGTYSKVIKTQFDYTQTQINT 60
           MG + GS+ W FIKQV AGRWFSVFA L+MM+G GTTY++ TYSKVIKTQFDY+QTQINT
Sbjct: 1   MGNKGGSEAWAFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT 60

Query: 61  LGFAKDLGSNLGIFAGLLAEVAPSSVLFFISAFLNFSCFFMIWLSITHRIARPKFWQIFL 120
           LGFAKDLGSN+GI AGLLAEV P+ VLF I AF NF+ FF+IWLSIT RIA+PKFWQ+F+
Sbjct: 61  LGFAKDLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFTGFFLIWLSITARIAKPKFWQMFI 120

Query: 121 YICVGTNSSNFANTAILVNGVRNFPDRRGVILGLLKGFVGIGGAVFTQLYLAFYGNDDPS 180
            +C  TNSSN+ANTAI+V  VRNFPDRRG+ILGLLKG+VGIGGA+ +QLYLA YG++DPS
Sbjct: 121 LVCFATNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIISQLYLAIYGHNDPS 180

Query: 181 NQVLLFSWLPSVLILILFSSIRPINSRKHPEELKVFYHLLYISILLALFILFLTIAQKQT 240
           N VLLF+WLPS+LILILF SIRPI  RKHP+ELKVFY LLY+SILLA+FILFLTIAQKQ 
Sbjct: 181 NLVLLFAWLPSLLILILFPSIRPIRIRKHPDELKVFYQLLYVSILLAIFILFLTIAQKQV 240

Query: 241 SFSHGGYVSGACVIIATLCLPLLIAFREEFFLYKLN-QNPHPNSSSSVVVSIIPEQQQAA 300
            FS  GYV GA  ++  LC+P+LIA REE  LYKLN Q   P+ + S++    P    +A
Sbjct: 241 VFSQAGYVGGASFVVCLLCVPILIACREELLLYKLNKQTAAPSVALSMLHQNPPTLPTSA 300

Query: 301 SKEITTL-----PTNSNKPARGDDFTILQAMFSKDMTLICLATFSGCGSSLAAIDNLGQI 360
             EI  +     P   NKP RG+DFTILQA+FSKDM LICLATFSGCGSSLAAIDNLGQ+
Sbjct: 301 DSEIPEISPSCCPNIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNLGQV 360

Query: 361 GESLHYPPQAISIFVSWVSIFSFFGRVCSGFISETLMVKYKLPRPLIFSFAFLITCIGQL 420
           GESL Y  +AI I VSWVSIF+FFGRV SGFISETLM KYKLPRPL F+FAFLITC+GQL
Sbjct: 361 GESLGYSQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFAFAFLITCVGQL 420

Query: 421 CIAFPSSGSVYAASLIIGFGFGSQVPLLFAIISELFGLKHYSTLFNWGQLIVPLGSYILN 480
            IA+P  GS+Y A++IIGFGFG+Q P+LFA+ISE+FGLK YSTLFN GQL  P GSYILN
Sbjct: 421 FIAYPFPGSLYLAAIIIGFGFGAQNPMLFAVISEMFGLKRYSTLFNCGQLAAPFGSYILN 480

Query: 481 VEVVGKLYDIEASKEHLRAGGIVGKGLTCNGAHCFSGSFTILASFTLFGALSMLVLAYRT 540
           V+VVGKLYD+EA +E  +   I GKGLTC GAHCF GSFT+LA+ TLFGA+ M VLAYRT
Sbjct: 481 VDVVGKLYDMEALRERKQ---IAGKGLTCTGAHCFGGSFTVLAAATLFGAVVMFVLAYRT 540

Query: 541 REFYRGDVYNKYKEDLWIQQSEMEFYHLEKKKKV 569
           REFY+ DVY  + E++WI Q+EMEFY L+ KK +
Sbjct: 541 REFYKRDVYKNFNEEIWIPQTEMEFYRLDNKKNI 571

BLAST of Sed0027618 vs. ExPASy TrEMBL
Match: A0A6J1HLS1 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111464770 PE=4 SV=1)

HSP 1 Score: 785.0 bits (2026), Expect = 2.1e-223
Identity = 400/574 (69.69%), Postives = 469/574 (81.71%), Query Frame = 0

Query: 1   MGWEEGSQVWGFIKQVCAGRWFSVFASLLMMMGTGTTYIYGTYSKVIKTQFDYTQTQINT 60
           MG + GS+ W FIKQV AGRWFSVFA L+MM+G GTTY++ TYSKVIKTQFDY+QTQINT
Sbjct: 1   MGNKGGSEAWAFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT 60

Query: 61  LGFAKDLGSNLGIFAGLLAEVAPSSVLFFISAFLNFSCFFMIWLSITHRIARPKFWQIFL 120
           LGFAKDLGSN+GI AGLLAEV P+ VLF I AF NF+ FF+IWLSIT RIA+PKFWQ+F+
Sbjct: 61  LGFAKDLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFTGFFLIWLSITARIAKPKFWQMFI 120

Query: 121 YICVGTNSSNFANTAILVNGVRNFPDRRGVILGLLKGFVGIGGAVFTQLYLAFYGNDDPS 180
            +C GTNSSN+ANTAI+V  VRNFPDRRG+ILGLLKG+VGIGGA+ +QLYLA YG++DPS
Sbjct: 121 LVCFGTNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIISQLYLAIYGHNDPS 180

Query: 181 NQVLLFSWLPSVLILILFSSIRPINSRKHPEELKVFYHLLYISILLALFILFLTIAQKQT 240
           N VLLF+WLPS+LILILF SIRPI  RKHP+ELKVFY LLY+SILLA+FILFLTIAQKQ 
Sbjct: 181 NLVLLFAWLPSLLILILFPSIRPIRIRKHPDELKVFYQLLYVSILLAIFILFLTIAQKQV 240

Query: 241 SFSHGGYVSGACVIIATLCLPLLIAFREEFFLYKLN-QNPHPNSSSSVVVSIIPEQQQAA 300
            FS  GYV GA  ++  LC+P+LIA REE  LYKLN +   P+ + S++    P    +A
Sbjct: 241 VFSQAGYVGGASFVVCLLCVPILIACREELLLYKLNKETAAPSVALSMLHQNPPTLPTSA 300

Query: 301 SKEITTL-----PTNSNKPARGDDFTILQAMFSKDMTLICLATFSGCGSSLAAIDNLGQI 360
             EI  +     P   NKP RG+DFTILQA+FSKDM LICLATFSGCGSSLAAIDNLGQ+
Sbjct: 301 DSEIPEISPSCCPNIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNLGQV 360

Query: 361 GESLHYPPQAISIFVSWVSIFSFFGRVCSGFISETLMVKYKLPRPLIFSFAFLITCIGQL 420
           GESL Y  +AI I VSWVSIF+FFGRV SGFISETLM KYKLPRPL F+FAFLITC+GQL
Sbjct: 361 GESLGYSQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFAFAFLITCVGQL 420

Query: 421 CIAFPSSGSVYAASLIIGFGFGSQVPLLFAIISELFGLKHYSTLFNWGQLIVPLGSYILN 480
            IA+P  GS+Y A++IIGFGFG+Q P+LFA+ISE+FGLK YSTLFN GQL  P GSYILN
Sbjct: 421 FIAYPFPGSLYLAAIIIGFGFGAQNPMLFAVISEMFGLKRYSTLFNCGQLAAPFGSYILN 480

Query: 481 VEVVGKLYDIEASKEHLRAGGIVGKGLTCNGAHCFSGSFTILASFTLFGALSMLVLAYRT 540
           V+VVGKLYD+EA +E  +   I GKGLTC GAHCF GSFT+LA+ TLFGA+ M VLAY+T
Sbjct: 481 VDVVGKLYDMEALRERKQ---IAGKGLTCTGAHCFGGSFTVLAAATLFGAVVMFVLAYQT 540

Query: 541 REFYRGDVYNKYKEDLWIQQSEMEFYHLEKKKKV 569
           REFY+ DVY  + E++WI Q+EMEFY L+ KK +
Sbjct: 541 REFYKRDVYKNFNEEIWIPQTEMEFYRLDNKKNI 571

BLAST of Sed0027618 vs. TAIR 10
Match: AT2G28120.1 (Major facilitator superfamily protein )

HSP 1 Score: 528.5 bits (1360), Expect = 6.7e-150
Identity = 286/543 (52.67%), Postives = 372/543 (68.51%), Query Frame = 0

Query: 19  GRWFSVFASLLMMMGTGTTYIYGTYSKVIKTQFDYTQTQINTLGFAKDLGSNLGIFAGLL 78
           GRWF VFAS L+M   G TY++GTYSK IK+   Y QT +N LGF KDLG+N+G+ +GL+
Sbjct: 16  GRWFMVFASFLIMACAGATYLFGTYSKDIKSTLGYDQTTLNLLGFFKDLGANVGVLSGLI 75

Query: 79  AEVAPSSVLFFISAFLNFSCFFMIWLSITHRIARPKFWQIFLYICVGTNSSNFANTAILV 138
           AEV P+  +  I + +NF  +FMIWL++T ++A+PK WQ+ LYIC+G NS NFANT  LV
Sbjct: 76  AEVTPTWFVLTIGSAMNFVGYFMIWLTVTGKVAKPKVWQMCLYICIGANSQNFANTGALV 135

Query: 139 NGVRNFPDRRGVILGLLKGFVGIGGAVFTQLYLAFYGNDDPSNQVLLFSWLPSVLILILF 198
             V+NFP+ RGV+LGLLKG+VG+ GA+FTQLY A YG+D  S  +LL +WLP+ + L+  
Sbjct: 136 TCVKNFPESRGVMLGLLKGYVGLSGAIFTQLYFAIYGHDSKS-LILLIAWLPAAVSLVFV 195

Query: 199 SSIRPINSRKHPEELKVFYHLLYISILLALFILFLTIAQKQTSFSHGGYVSGACVIIATL 258
             IR     +   EL VFY  LYISI LALF++ + IA+KQ  FS   Y + A +  A L
Sbjct: 196 YLIREKKVVRQRNELSVFYQFLYISIFLALFLMAMNIAEKQVHFSKAAYAASATICCALL 255

Query: 259 CLPLLIAFREEFFLYKLNQNPHPNSSSSVV----VSIIPEQQQAASKEITTLPTNS---- 318
            +PL ++ ++E  ++ + + P    S   V      +  +Q +AA        T S    
Sbjct: 256 FVPLTVSVKQELEVWNMMKLPIEEPSEVKVEKPKKELDLDQDKAAKVNGEEKETKSCFST 315

Query: 319 --NKPARGDDFTILQAMFSKDMTLICLATFSGCGSSLAAIDNLGQIGESLHYPPQAISIF 378
             + P RG+D+TILQA+ S DM ++ +ATF G GSSL A+DNLGQIGESL YP   +S F
Sbjct: 316 VFSPPPRGEDYTILQALLSTDMIILFVATFCGLGSSLTAVDNLGQIGESLGYPNHTVSSF 375

Query: 379 VSWVSIFSFFGRVCSGFISETLMVKYKLPRPLIFSFAFLITCIGQLCIAFPSSGSVYAAS 438
           VS VSI+++FGRV SGF+SE L+ KYKLPRPL+ +   L++C G L IAFP  GSVY AS
Sbjct: 376 VSLVSIWNYFGRVFSGFVSEYLLAKYKLPRPLMMTLVLLLSCAGHLLIAFPVPGSVYIAS 435

Query: 439 LIIGFGFGSQVPLLFAIISELFGLKHYSTLFNWGQLIVPLGSYILNVEVVGKLYDIEASK 498
           +++GF FG+Q+PLLFAIISELFGLK+YSTLFN GQL  PLGSYILNV V G LYD EA K
Sbjct: 436 ILMGFSFGAQLPLLFAIISELFGLKYYSTLFNCGQLASPLGSYILNVRVTGMLYDKEALK 495

Query: 499 EHLRAGGIVG---KGLTCNGAHCFSGSFTILASFTLFGALSMLVLAYRTREFYRGDVYNK 549
           + L A G+     K LTC G+ C+   F ILA+ T FGAL  L LA RTREFY+GD+Y K
Sbjct: 496 Q-LTARGLTRKDVKDLTCLGSQCYKLPFLILAAVTFFGALVSLGLAIRTREFYKGDIYKK 555

BLAST of Sed0027618 vs. TAIR 10
Match: AT2G39210.1 (Major facilitator superfamily protein )

HSP 1 Score: 498.8 bits (1283), Expect = 5.7e-141
Identity = 266/560 (47.50%), Postives = 362/560 (64.64%), Query Frame = 0

Query: 15  QVCAGRWFSVFASLLMMMGTGTTYIYGTYSKVIKTQFDYTQTQINTLGFAKDLGSNLGIF 74
           Q+  GRWF  F SLL+M   G TY++G YS  IK    Y QT +N L F KDLG+N+G+ 
Sbjct: 16  QILTGRWFMFFGSLLIMSTAGATYMFGIYSGDIKETLGYDQTTLNLLSFFKDLGANVGVL 75

Query: 75  AGLLAEVAPSSVLFFISAFLNFSCFFMIWLSITHRIARPKFWQIFLYICVGTNSSNFANT 134
           AGLL EV P   +  I A LNF  +FMIWL++T RI++P+ W + LYICVG NS +FANT
Sbjct: 76  AGLLNEVTPPWFILLIGAILNFFGYFMIWLAVTERISKPQVWHMCLYICVGANSQSFANT 135

Query: 135 AILVNGVRNFPDRRGVILGLLKGFVGIGGAVFTQLYLAFYGNDDPSNQVLLFSWLPSVLI 194
             LV  V+NFP+ RGV+LG+LKG+VG+ GA+ TQLY AFYG +D    +L+  WLP+++ 
Sbjct: 136 GSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLYRAFYG-EDTKELILMIGWLPAIVS 195

Query: 195 LILFSSIRPINSRKHPEELKVFYHLLYISILLALFILFLTIAQKQTSFSHGGYVSGACVI 254
                +IR +  ++   ELKVFY+ LYIS+ LA F++ + I  K + F+   +   A V+
Sbjct: 196 FAFLRTIRIMKVKRQTNELKVFYNFLYISLGLATFLMVVIIINKLSGFTQSEFGGSAAVV 255

Query: 255 IATLCLPLLIAFREEFFLYKLNQ----NPHP----NSSSSVVVSIIPEQQQAASKEI--- 314
           I  L LP+++   EE  L+K  Q    +P P         +  S   +     SKE+   
Sbjct: 256 IVLLLLPIIVVILEEKKLWKEKQVALNDPAPINVVTEKPKLDSSEFKDDDGEESKEVVEK 315

Query: 315 ----TTLPTNSNKPARGDDFTILQAMFSKDMTLICLATFSGCGSSLAAIDNLGQIGESLH 374
               +   T  N P RGDD+TILQA+FS DM ++ LAT  G G +L AIDNLGQIG SL 
Sbjct: 316 VKTPSCWTTVFNPPERGDDYTILQALFSVDMLILFLATICGVGGTLTAIDNLGQIGNSLG 375

Query: 375 YPPQAISIFVSWVSIFSFFGRVCSGFISETLMVKYKLPRPLIFSFAFLITCIGQLCIAFP 434
           YP +++S FVS VSI++++GRV SG +SE  ++KYK PRPL+ +   L++C G L IAF 
Sbjct: 376 YPKRSVSTFVSLVSIWNYYGRVVSGVVSEIFLIKYKFPRPLMLTMVLLLSCAGHLLIAFN 435

Query: 435 SSGSVYAASLIIGFGFGSQVPLLFAIISELFGLKHYSTLFNWGQLIVPLGSYILNVEVVG 494
             G +Y AS+IIGF FG+Q PLLFAIISE+FGLK+YSTL+N+G +  P+GSY+LNV V G
Sbjct: 436 VPGGLYVASVIIGFCFGAQWPLLFAIISEIFGLKYYSTLYNFGSVASPIGSYLLNVRVAG 495

Query: 495 KLYDIEASKEHLRAG--GIVGKGLTCNGAHCFSGSFTILASFTLFGALSMLVLAYRTREF 554
            LYD+EA K++   G   + G+ L C G  CF  SF I+A+ TLFG L  +VL  RT++F
Sbjct: 496 YLYDVEAGKQYKALGKTRVEGQDLNCIGTSCFKLSFIIIAAVTLFGVLVSMVLVIRTKKF 555

Query: 555 YRGDVYNKYKEDLWIQQSEM 558
           Y+ D+Y K++E     + EM
Sbjct: 556 YKSDIYKKFREKALAAEMEM 574

BLAST of Sed0027618 vs. TAIR 10
Match: AT5G14120.1 (Major facilitator superfamily protein )

HSP 1 Score: 270.4 bits (690), Expect = 3.3e-72
Identity = 168/560 (30.00%), Postives = 280/560 (50.00%), Query Frame = 0

Query: 20  RWFSVFASLLMMMGTGTTYIYGTYSKVIKTQFDYTQTQINTLGFAKDLGSNLGIFAGLLA 79
           RW    A++ +    G  Y++G+ S VIK+  +Y Q +++ LG AKDLG ++G  AG L+
Sbjct: 16  RWLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKELSRLGVAKDLGDSVGFIAGTLS 75

Query: 80  EVAPSSVLFFISAFLNFSCFFMIWLSITHRIARPKFWQIFLYICVGTNSSNFANTAILVN 139
           E+ P      + A  N   +  +WL +T R      W + + I VG N   + NT  LV+
Sbjct: 76  EILPLWAALLVGAVQNLIGYGWVWLIVTGRAPILPLWAMCVLIFVGNNGETYFNTGALVS 135

Query: 140 GVRNFPDRRGVILGLLKGFVGIGGAVFTQLYLAFYGNDDPSNQVLLFSWLPSVLILILFS 199
           GV+NFP  RG ++G+LKGF G+GGA+ +Q+Y   + + +P++ +L+ +  P+V+++ L  
Sbjct: 136 GVQNFPKSRGPVVGILKGFAGLGGAIISQIYTMIH-SSNPASLILMVAVTPAVVVVCLMF 195

Query: 200 SIRPINSRK--HPEELKVFYHLLYISILLALFILFLTIAQKQTSFSHGGYVSGACVIIAT 259
            IRP+   K   P +   F  +  + +LLA +++ + + Q     SH        V+   
Sbjct: 196 FIRPVGGHKQIRPTDGASFTFIYGVCLLLAAYLMSVMLIQDLVVVSHNVITVFTIVLFVI 255

Query: 260 LCLPLLIAFREEFF-------------LYKLNQNPHPNSSSSVVVSIIPEQQQAASKEIT 319
           L +P+L+     FF             L    ++  P   +  +  I+ E +    K++ 
Sbjct: 256 LVVPILVPIMTSFFTETNEPDDTIEEPLVPKREDQEPGLQTPDL--ILSEVEDEKPKDVD 315

Query: 320 TLPTNSNK--------------------------PARGDDFTILQAMFSKDMTLICLATF 379
            LP +                             P RG+DFT+ QA+   D  LI  +  
Sbjct: 316 LLPASERHKRIAHLQAQLMQAAAEGAVRVNRRRGPHRGEDFTLTQALVKADFWLIFFSLL 375

Query: 380 SGCGSSLAAIDNLGQIGESLHYPPQAISIFVSWVSIFSFFGRVCSGFISETLMVKYKLPR 439
            G GS L  IDNLGQ+ +SL Y      + VS +SI++F GR+  G+ SE ++  Y  PR
Sbjct: 376 LGSGSGLTVIDNLGQMSQSLGY--DNTHVLVSMISIWNFLGRIGGGYFSELVVRDYAYPR 435

Query: 440 PLIFSFAFLITCIGQLCIAFPSSGSVYAASLIIGFGFGSQVPLLFAIISELFGLKHYSTL 499
           P+  + A LI  +G +  A+   G++Y  +L+IG G+G+   ++ A  SELFGLK +  L
Sbjct: 436 PVAMAVAQLIMSVGHIFFAYGWPGAMYIGTLLIGLGYGAHWAIVPATASELFGLKKFGAL 495

Query: 500 FNWGQLIVPLGSYILNVEVVGKLYDIEASKEHLRAGGIVGKGLTCNGAHCFSGSFTILAS 539
           +N+  L  P GS + +  +   +YD EA ++   +       L CNG+ CF  +  I++ 
Sbjct: 496 YNFLTLANPAGSLVFSGMIASSIYDREAERQAHGSVFDPDDALRCNGSICFFLTSLIMSG 555

BLAST of Sed0027618 vs. TAIR 10
Match: AT5G50630.1 (Major facilitator superfamily protein )

HSP 1 Score: 270.0 bits (689), Expect = 4.3e-72
Identity = 173/534 (32.40%), Postives = 282/534 (52.81%), Query Frame = 0

Query: 20  RWFSVFASLLMMMGTGTTYIY-GTYSKVIKTQFDYTQTQINTLGFAKDLGSNLGIFAGLL 79
           RW     ++ +    G  Y++ G+ S  IKT   Y Q QI  LG AK+LG  +G  +G L
Sbjct: 16  RWLVFVCAMWVQSVAGVGYLFGGSMSPAIKTSLGYNQKQIALLGVAKNLGDAIGFVSGAL 75

Query: 80  AEVAPSSVLFFISAFLNFSCFFMIWLSITHRIARPKFWQIFLYICVGTNSSNFANTAILV 139
           +EV+PS V+  + A  N   + ++WL +T ++     W +F+ I VGTN   + NTA LV
Sbjct: 76  SEVSPSWVVLLVGATQNLFGYGVVWLVVTGQLPNLPLWMLFVAIFVGTNGETYYNTASLV 135

Query: 140 NGVRNFPDRRGVILGLLKGFVGIGGAVFTQLYLAFYGNDDPSNQVLLFSWLPSVLILILF 199
           + + NFP+ RG ++G+LKGF G+ GA+ TQ+YL F  + D S+ +L+ +  P V++L L 
Sbjct: 136 SCIHNFPESRGPVVGILKGFSGLSGAILTQVYLMFNPSHD-SSVILMVALGPPVVVLALL 195

Query: 200 SSIRPI----NSRKHPEELKVFYHLLYISILLALFILFLTIAQKQTSFSHGGYVSGACVI 259
             +RP+     +    ++L+ F  +    ++LA+++L L + Q     +     +   ++
Sbjct: 196 FIVRPVERSCRTNLRSDDLR-FLAIYGFCVVLAVYLLGLLVLQSVFDMTQTIITTSGAIL 255

Query: 260 IATLCLPLLIAFREEFFLYKLNQNPHPNSSSSVVVSIIPEQ--QQAASKEITTL------ 319
           +  + +P+L+ F   F             S + V S+ PE+        E  TL      
Sbjct: 256 VIFMVVPVLVPFSSVFI------------SGNNVTSVKPEEGTSNVDQHEARTLIERSDR 315

Query: 320 -PTNSNKPARGDDFTILQAMFSKDMTLICLATFSGCGSSLAAIDNLGQIGESLHYPPQAI 379
            P     P  G+DFT+LQA+   D  LI ++   G GS +  IDNLGQI  SL Y     
Sbjct: 316 PPEKKRAPCIGEDFTLLQALGQADFWLIFMSLVLGVGSGITIIDNLGQICYSLGY--SNT 375

Query: 380 SIFVSWVSIFSFFGRVCSGFISETLMVKYKLPRPLIFSFAFLITCIGQLCIAFPSSGSVY 439
            IFVS +SI +F GRV  G+ SE ++ K  LPR L  S    I  +G +  A    G +Y
Sbjct: 376 KIFVSLISISNFLGRVAGGYFSELIIRKLSLPRTLAMSVVQAIMSLGLIYYAIDWPGKIY 435

Query: 440 AASLIIGFGFGSQVPLLFAIISELFGLKHYSTLFNWGQLIVPLGSYILNVEVVGKLYDIE 499
             +++IG G+G+   +  A +S++FGLK + +L+N+    +P+GS++ +  +   +YD  
Sbjct: 436 VVTIVIGMGYGAHWAIAPASVSDIFGLKSFGSLYNFQITALPIGSFVFSGVIASNIYDYY 495

Query: 500 ASKEHLRAGGIV-GKGLTCNGAHCFSGSFTILASFTLFGALSMLVLAYRTREFY 539
           A K+   AG     + L C G+ C+S + ++++   L   +  L + YRTR+FY
Sbjct: 496 ARKQ---AGPTTETESLVCTGSVCYSVTCSLMSMLCLMAMVLSLSVVYRTRKFY 530

BLAST of Sed0027618 vs. TAIR 10
Match: AT5G50520.1 (Major facilitator superfamily protein )

HSP 1 Score: 270.0 bits (689), Expect = 4.3e-72
Identity = 173/534 (32.40%), Postives = 282/534 (52.81%), Query Frame = 0

Query: 20  RWFSVFASLLMMMGTGTTYIY-GTYSKVIKTQFDYTQTQINTLGFAKDLGSNLGIFAGLL 79
           RW     ++ +    G  Y++ G+ S  IKT   Y Q QI  LG AK+LG  +G  +G L
Sbjct: 16  RWLVFVCAMWVQSVAGVGYLFGGSMSPAIKTSLGYNQKQIALLGVAKNLGDAIGFVSGAL 75

Query: 80  AEVAPSSVLFFISAFLNFSCFFMIWLSITHRIARPKFWQIFLYICVGTNSSNFANTAILV 139
           +EV+PS V+  + A  N   + ++WL +T ++     W +F+ I VGTN   + NTA LV
Sbjct: 76  SEVSPSWVVLLVGATQNLFGYGVVWLVVTGQLPNLPLWMLFVAIFVGTNGETYYNTASLV 135

Query: 140 NGVRNFPDRRGVILGLLKGFVGIGGAVFTQLYLAFYGNDDPSNQVLLFSWLPSVLILILF 199
           + + NFP+ RG ++G+LKGF G+ GA+ TQ+YL F  + D S+ +L+ +  P V++L L 
Sbjct: 136 SCIHNFPESRGPVVGILKGFSGLSGAILTQVYLMFNPSHD-SSVILMVALGPPVVVLALL 195

Query: 200 SSIRPI----NSRKHPEELKVFYHLLYISILLALFILFLTIAQKQTSFSHGGYVSGACVI 259
             +RP+     +    ++L+ F  +    ++LA+++L L + Q     +     +   ++
Sbjct: 196 FIVRPVERSCRTNLRSDDLR-FLAIYGFCVVLAVYLLGLLVLQSVFDMTQTIITTSGAIL 255

Query: 260 IATLCLPLLIAFREEFFLYKLNQNPHPNSSSSVVVSIIPEQ--QQAASKEITTL------ 319
           +  + +P+L+ F   F             S + V S+ PE+        E  TL      
Sbjct: 256 VIFMVVPVLVPFSSVFI------------SGNNVTSVKPEEGTSNVDQHEARTLIERSDR 315

Query: 320 -PTNSNKPARGDDFTILQAMFSKDMTLICLATFSGCGSSLAAIDNLGQIGESLHYPPQAI 379
            P     P  G+DFT+LQA+   D  LI ++   G GS +  IDNLGQI  SL Y     
Sbjct: 316 PPEKKRAPCIGEDFTLLQALGQADFWLIFMSLVLGVGSGITIIDNLGQICYSLGY--SNT 375

Query: 380 SIFVSWVSIFSFFGRVCSGFISETLMVKYKLPRPLIFSFAFLITCIGQLCIAFPSSGSVY 439
            IFVS +SI +F GRV  G+ SE ++ K  LPR L  S    I  +G +  A    G +Y
Sbjct: 376 KIFVSLISISNFLGRVAGGYFSELIIRKLSLPRTLAMSVVQAIMSLGLIYYAIDWPGKIY 435

Query: 440 AASLIIGFGFGSQVPLLFAIISELFGLKHYSTLFNWGQLIVPLGSYILNVEVVGKLYDIE 499
             +++IG G+G+   +  A +S++FGLK + +L+N+    +P+GS++ +  +   +YD  
Sbjct: 436 VVTIVIGMGYGAHWAIAPASVSDIFGLKSFGSLYNFQITALPIGSFVFSGVIASNIYDYY 495

Query: 500 ASKEHLRAGGIV-GKGLTCNGAHCFSGSFTILASFTLFGALSMLVLAYRTREFY 539
           A K+   AG     + L C G+ C+S + ++++   L   +  L + YRTR+FY
Sbjct: 496 ARKQ---AGPTTETESLVCTGSVCYSVTCSLMSMLCLMAMVLSLSVVYRTRKFY 530