Sed0027463 (gene) Chayote v1

BLAST of Sed0027463 vs. NCBI nr
Match: XP_022991622.1 (tRNA:m(4)X modification enzyme TRM13 homolog [Cucurbita maxima])

HSP 1 Score: 838.2 bits (2164), Expect = 3.3e-239
Identity = 403/457 (88.18%), Postives = 424/457 (92.78%), Query Frame = 0

Query: 1   MASRCQFWLPKKSRFCSNPPFNDSKFCGNHSARADGQWVPCPVDSSHSVLQENLEWHVKR 60
           MASRCQFWLPKK+RFC+N P NDSKFCGNHS+RAD QWVPCPVD SHSVL+ENLEWHVKR
Sbjct: 1   MASRCQFWLPKKNRFCANSPINDSKFCGNHSSRADTQWVPCPVDPSHSVLRENLEWHVKR 60

Query: 61  CPLLKQAQSLAVQPFYQKGINAGEEDSTLSNEPASSLSDSISSEMKRNIVHGMSGAEFHR 120
           CPLLKQ QSLAVQPFYQKGINAGEEDS  S+E AS LSDSISSEMKRN+V+GMSGAEFHR
Sbjct: 61  CPLLKQTQSLAVQPFYQKGINAGEEDSISSDELASGLSDSISSEMKRNVVYGMSGAEFHR 120

Query: 121 LLGKIRALHGVICKDIQDSYRIKDACNMWIKGEIDRKIPFQEKHVLQQASILGNMEEFGV 180
           LL KIRALHG+ICKDIQDSYRIK+ACNMWIKGEIDRKIPFQEKHVLQQASILGNMEEFGV
Sbjct: 121 LLAKIRALHGLICKDIQDSYRIKEACNMWIKGEIDRKIPFQEKHVLQQASILGNMEEFGV 180

Query: 181 LRNYDGGEQC----------NVVPAVVEFGAGRGYLTQMLADCYGIKRVFLVERKSYKLK 240
           LRNYDGGEQC          NV PAV+EFGAGRGYLTQMLADCYGIKRV LVERKSYKLK
Sbjct: 181 LRNYDGGEQCEGNRSCGNEANVAPAVIEFGAGRGYLTQMLADCYGIKRVLLVERKSYKLK 240

Query: 241 ADRSLRQKESLILERLRIDIEDLNLNAVESLRRIPYIAIGKHLCGPATDLALRCCLTKQT 300
           ADRSLRQKESL+LERLRIDIEDLNLNAVESLR  PY+AIGKHLCGPATDLALRCCLTKQ+
Sbjct: 241 ADRSLRQKESLVLERLRIDIEDLNLNAVESLRHNPYLAIGKHLCGPATDLALRCCLTKQS 300

Query: 301 NHVNTEQCGDSPKLEGLAIATCCHHLCQWKHYTNKRYLLELGMTKEEFLAITWFTSWAVD 360
           NHVNTEQCGD PKL GLAIATCCHHLCQWKHYTNKRYLLELG+TKEEFLAITWFTSWAVD
Sbjct: 301 NHVNTEQCGDRPKLRGLAIATCCHHLCQWKHYTNKRYLLELGITKEEFLAITWFTSWAVD 360

Query: 361 ANHSEAISVVTDSKSYLQPSENKGDGTNENTVEDIVRNMNPVERAMLGFMCKEIIDMGRL 420
           ANHSE IS V DSK++LQ SEN+GDG N +TVEDI R+MNPVERA+LGFMCKEIIDMGRL
Sbjct: 361 ANHSEDISDVADSKTFLQSSENEGDGMNGSTVEDIARHMNPVERAVLGFMCKEIIDMGRL 420

Query: 421 MWLKECGLETQLVKYVPSNISPENHLLIAKCRNICAA 448
           MWLKECGLETQLVKYVPS+ISPENHLLIAKCRNICAA
Sbjct: 421 MWLKECGLETQLVKYVPSSISPENHLLIAKCRNICAA 457

BLAST of Sed0027463 vs. NCBI nr
Match: KAG7014284.1 (tRNA:m(4)X modification enzyme TRM13-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 836.3 bits (2159), Expect = 1.3e-238
Identity = 402/457 (87.96%), Postives = 424/457 (92.78%), Query Frame = 0

Query: 1   MASRCQFWLPKKSRFCSNPPFNDSKFCGNHSARADGQWVPCPVDSSHSVLQENLEWHVKR 60
           MASRCQFWLPKK+RFC+N P NDSKFCGNHS+RAD QWVPCPVD SHSVL+ENLEWHVKR
Sbjct: 14  MASRCQFWLPKKNRFCANSPINDSKFCGNHSSRADTQWVPCPVDPSHSVLRENLEWHVKR 73

Query: 61  CPLLKQAQSLAVQPFYQKGINAGEEDSTLSNEPASSLSDSISSEMKRNIVHGMSGAEFHR 120
           CPLLKQ QSLAVQPFYQKGINAGEEDS  S+E AS LSD ISSEMKRNIV+GMSGAEFHR
Sbjct: 74  CPLLKQTQSLAVQPFYQKGINAGEEDSISSDELASGLSDGISSEMKRNIVYGMSGAEFHR 133

Query: 121 LLGKIRALHGVICKDIQDSYRIKDACNMWIKGEIDRKIPFQEKHVLQQASILGNMEEFGV 180
           LL KIRALHG+ICKDIQDSYRIK+ACNMWIKGEIDRKIPFQEKHVLQQASILGNMEEFGV
Sbjct: 134 LLAKIRALHGLICKDIQDSYRIKEACNMWIKGEIDRKIPFQEKHVLQQASILGNMEEFGV 193

Query: 181 LRNYDGGEQC----------NVVPAVVEFGAGRGYLTQMLADCYGIKRVFLVERKSYKLK 240
           LRNYDGGEQC          NV PAV+EFGAGRGYLTQMLADCYGIKRV LVERKSYKLK
Sbjct: 194 LRNYDGGEQCEGNRSCGNEANVAPAVIEFGAGRGYLTQMLADCYGIKRVLLVERKSYKLK 253

Query: 241 ADRSLRQKESLILERLRIDIEDLNLNAVESLRRIPYIAIGKHLCGPATDLALRCCLTKQT 300
           ADRSLRQKESL+LERLRIDIEDLNLNAVESLR  PY+AIGKHLCGPATDLALRCCLTKQ+
Sbjct: 254 ADRSLRQKESLVLERLRIDIEDLNLNAVESLRHDPYLAIGKHLCGPATDLALRCCLTKQS 313

Query: 301 NHVNTEQCGDSPKLEGLAIATCCHHLCQWKHYTNKRYLLELGMTKEEFLAITWFTSWAVD 360
           NHVNTEQCGD PKL GLAIATCCHHLCQWKHYTNKRYLLELG+TKEEFLAITWFTSWAVD
Sbjct: 314 NHVNTEQCGDRPKLRGLAIATCCHHLCQWKHYTNKRYLLELGITKEEFLAITWFTSWAVD 373

Query: 361 ANHSEAISVVTDSKSYLQPSENKGDGTNENTVEDIVRNMNPVERAMLGFMCKEIIDMGRL 420
           ANHSE IS V+DSK++LQ SEN+GDG N +TVEDI ++MNPVERA+LGFMCKEIIDMGRL
Sbjct: 374 ANHSEDISDVSDSKTFLQSSENEGDGMNGSTVEDIAKHMNPVERAVLGFMCKEIIDMGRL 433

Query: 421 MWLKECGLETQLVKYVPSNISPENHLLIAKCRNICAA 448
           MWLKECGLETQLVKYVPS+ISPENHLLIAKCRNICAA
Sbjct: 434 MWLKECGLETQLVKYVPSSISPENHLLIAKCRNICAA 470

BLAST of Sed0027463 vs. NCBI nr
Match: XP_022954384.1 (tRNA:m(4)X modification enzyme TRM13 homolog [Cucurbita moschata])

HSP 1 Score: 835.9 bits (2158), Expect = 1.7e-238
Identity = 402/457 (87.96%), Postives = 424/457 (92.78%), Query Frame = 0

Query: 1   MASRCQFWLPKKSRFCSNPPFNDSKFCGNHSARADGQWVPCPVDSSHSVLQENLEWHVKR 60
           MASRCQFWLPKK+RFC+N P NDSKFCGNHS+RAD QWVPCPVD SHSVL+ENLEWHVKR
Sbjct: 1   MASRCQFWLPKKNRFCANSPINDSKFCGNHSSRADTQWVPCPVDPSHSVLRENLEWHVKR 60

Query: 61  CPLLKQAQSLAVQPFYQKGINAGEEDSTLSNEPASSLSDSISSEMKRNIVHGMSGAEFHR 120
           CPLLKQ QSLAVQPFYQKGINAGEEDS  S+E AS LSDSISSEMKRNIV+GMSGAEFHR
Sbjct: 61  CPLLKQTQSLAVQPFYQKGINAGEEDSISSDELASGLSDSISSEMKRNIVYGMSGAEFHR 120

Query: 121 LLGKIRALHGVICKDIQDSYRIKDACNMWIKGEIDRKIPFQEKHVLQQASILGNMEEFGV 180
           LL KIRALHG+ICKDIQDSYRIK+ACNMWIKGEIDRKIPFQEKHVLQQASILGNMEEFGV
Sbjct: 121 LLAKIRALHGLICKDIQDSYRIKEACNMWIKGEIDRKIPFQEKHVLQQASILGNMEEFGV 180

Query: 181 LRNYDGGEQC----------NVVPAVVEFGAGRGYLTQMLADCYGIKRVFLVERKSYKLK 240
           LRNYDGGEQC          NV PAV+EFGAGRGYLTQMLADCYGIKRV LVERKSYKLK
Sbjct: 181 LRNYDGGEQCEGNRSCGNEANVAPAVIEFGAGRGYLTQMLADCYGIKRVLLVERKSYKLK 240

Query: 241 ADRSLRQKESLILERLRIDIEDLNLNAVESLRRIPYIAIGKHLCGPATDLALRCCLTKQT 300
           ADRSLRQKESL+LERLRIDIEDLNLNAVESLR  PY+AIGKHLCGPATDLALRCCLTKQ+
Sbjct: 241 ADRSLRQKESLVLERLRIDIEDLNLNAVESLRHDPYLAIGKHLCGPATDLALRCCLTKQS 300

Query: 301 NHVNTEQCGDSPKLEGLAIATCCHHLCQWKHYTNKRYLLELGMTKEEFLAITWFTSWAVD 360
           NHVNTEQCGD PKL GLAIATCCHHLCQWKHYTNK+YLLELG+ KEEFLAITWFTSWAVD
Sbjct: 301 NHVNTEQCGDRPKLRGLAIATCCHHLCQWKHYTNKKYLLELGIAKEEFLAITWFTSWAVD 360

Query: 361 ANHSEAISVVTDSKSYLQPSENKGDGTNENTVEDIVRNMNPVERAMLGFMCKEIIDMGRL 420
           ANHSE IS V+DSK++LQ SEN+GDG N +TVEDI R+MNPVERA+LGFMCKEIIDMGRL
Sbjct: 361 ANHSEDISDVSDSKTFLQSSENEGDGMNGSTVEDIARHMNPVERAVLGFMCKEIIDMGRL 420

Query: 421 MWLKECGLETQLVKYVPSNISPENHLLIAKCRNICAA 448
           MWLKECGLETQLVKYVPS+ISPENHLLIAKCRNICAA
Sbjct: 421 MWLKECGLETQLVKYVPSSISPENHLLIAKCRNICAA 457

BLAST of Sed0027463 vs. NCBI nr
Match: KAG6575728.1 (tRNA:m(4)X modification enzyme TRM13, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 835.9 bits (2158), Expect = 1.7e-238
Identity = 401/457 (87.75%), Postives = 424/457 (92.78%), Query Frame = 0

Query: 1   MASRCQFWLPKKSRFCSNPPFNDSKFCGNHSARADGQWVPCPVDSSHSVLQENLEWHVKR 60
           MASRCQFWLPKK+RFC+N P +DSKFCGNHS+RAD QWVPCPVD SHSVL+ENLEWHVKR
Sbjct: 14  MASRCQFWLPKKNRFCANSPISDSKFCGNHSSRADTQWVPCPVDPSHSVLRENLEWHVKR 73

Query: 61  CPLLKQAQSLAVQPFYQKGINAGEEDSTLSNEPASSLSDSISSEMKRNIVHGMSGAEFHR 120
           CPLLKQ QSLAVQPFYQKGINAGEEDS  SNE AS LSD ISSEMKRNIV+GMSGAEFHR
Sbjct: 74  CPLLKQTQSLAVQPFYQKGINAGEEDSISSNELASGLSDGISSEMKRNIVYGMSGAEFHR 133

Query: 121 LLGKIRALHGVICKDIQDSYRIKDACNMWIKGEIDRKIPFQEKHVLQQASILGNMEEFGV 180
           LL KIRALHG+ICKDIQDSYRIK+ACNMWIKGEIDRKIPFQEKHVLQQASILGNMEEFGV
Sbjct: 134 LLAKIRALHGLICKDIQDSYRIKEACNMWIKGEIDRKIPFQEKHVLQQASILGNMEEFGV 193

Query: 181 LRNYDGGEQC----------NVVPAVVEFGAGRGYLTQMLADCYGIKRVFLVERKSYKLK 240
           LRNYDGGEQC          NV PAV+EFGAGRGYLTQMLADCYGIKRV LVERKSYKLK
Sbjct: 194 LRNYDGGEQCEGNRSCGNEANVAPAVIEFGAGRGYLTQMLADCYGIKRVLLVERKSYKLK 253

Query: 241 ADRSLRQKESLILERLRIDIEDLNLNAVESLRRIPYIAIGKHLCGPATDLALRCCLTKQT 300
           ADRSLRQKESL+LERLRIDIEDLNLNAVESLR  PY+AIGKHLCGPATDLALRCCLTKQ+
Sbjct: 254 ADRSLRQKESLVLERLRIDIEDLNLNAVESLRHDPYLAIGKHLCGPATDLALRCCLTKQS 313

Query: 301 NHVNTEQCGDSPKLEGLAIATCCHHLCQWKHYTNKRYLLELGMTKEEFLAITWFTSWAVD 360
           NHVNTEQCGD PKL GLAIATCCHHLCQWKHYTNKRYLLELG+TKEEFLAITWFTSWAVD
Sbjct: 314 NHVNTEQCGDRPKLRGLAIATCCHHLCQWKHYTNKRYLLELGITKEEFLAITWFTSWAVD 373

Query: 361 ANHSEAISVVTDSKSYLQPSENKGDGTNENTVEDIVRNMNPVERAMLGFMCKEIIDMGRL 420
           ANHSE IS V+DSK++LQ SEN+GDG N +TVEDI ++MNPVERA+LGFMCKEIIDMGRL
Sbjct: 374 ANHSEDISDVSDSKTFLQSSENEGDGMNGSTVEDIAKHMNPVERAVLGFMCKEIIDMGRL 433

Query: 421 MWLKECGLETQLVKYVPSNISPENHLLIAKCRNICAA 448
           MWLKECGLETQLVKYVPS+ISPENHLL+AKCRNICAA
Sbjct: 434 MWLKECGLETQLVKYVPSSISPENHLLVAKCRNICAA 470

BLAST of Sed0027463 vs. NCBI nr
Match: XP_023547814.1 (tRNA:m(4)X modification enzyme TRM13 homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 832.0 bits (2148), Expect = 2.4e-237
Identity = 401/457 (87.75%), Postives = 421/457 (92.12%), Query Frame = 0

Query: 1   MASRCQFWLPKKSRFCSNPPFNDSKFCGNHSARADGQWVPCPVDSSHSVLQENLEWHVKR 60
           MASRCQFWLPKK+RFC+N P NDSKFCGNHS+RAD QWVPCPVD SHSVL+ENLEWHVKR
Sbjct: 14  MASRCQFWLPKKNRFCANSPINDSKFCGNHSSRADTQWVPCPVDPSHSVLRENLEWHVKR 73

Query: 61  CPLLKQAQSLAVQPFYQKGINAGEEDSTLSNEPASSLSDSISSEMKRNIVHGMSGAEFHR 120
           CPLLKQ QSLAVQPFYQKGINAGEEDS  S+E AS  SDSISSEMKRNIV+GMSGAEFHR
Sbjct: 74  CPLLKQTQSLAVQPFYQKGINAGEEDSISSDELASGFSDSISSEMKRNIVYGMSGAEFHR 133

Query: 121 LLGKIRALHGVICKDIQDSYRIKDACNMWIKGEIDRKIPFQEKHVLQQASILGNMEEFGV 180
           LL KIRALHG+ICKDIQDSYRIK+ACNMWIKGEIDRKIPFQEKHVLQQASILGNMEEFGV
Sbjct: 134 LLAKIRALHGLICKDIQDSYRIKEACNMWIKGEIDRKIPFQEKHVLQQASILGNMEEFGV 193

Query: 181 LRNYDGGEQC----------NVVPAVVEFGAGRGYLTQMLADCYGIKRVFLVERKSYKLK 240
           LRNYDGGEQC          NV PAV+EFGAGRGYLTQMLADCYGIKRV LVERKSYKLK
Sbjct: 194 LRNYDGGEQCEGNRSCGNEANVAPAVIEFGAGRGYLTQMLADCYGIKRVLLVERKSYKLK 253

Query: 241 ADRSLRQKESLILERLRIDIEDLNLNAVESLRRIPYIAIGKHLCGPATDLALRCCLTKQT 300
           ADRSLRQKESL+LERLRIDIEDLNLNAVESLR  PY+AIGKHLCGPATDLALRCCLTKQ+
Sbjct: 254 ADRSLRQKESLVLERLRIDIEDLNLNAVESLRHNPYLAIGKHLCGPATDLALRCCLTKQS 313

Query: 301 NHVNTEQCGDSPKLEGLAIATCCHHLCQWKHYTNKRYLLELGMTKEEFLAITWFTSWAVD 360
           NHVNTEQCGD PKL GLAIATCCHHLCQWKHYTNKRYLLELG+TKEEFLAITWFTSWAVD
Sbjct: 314 NHVNTEQCGDRPKLRGLAIATCCHHLCQWKHYTNKRYLLELGITKEEFLAITWFTSWAVD 373

Query: 361 ANHSEAISVVTDSKSYLQPSENKGDGTNENTVEDIVRNMNPVERAMLGFMCKEIIDMGRL 420
           ANHSE IS   D K++LQ SEN+GDG N +TVEDI R+MNPVERA+LGFMCKEIIDMGRL
Sbjct: 374 ANHSEDISDGADLKTFLQSSENEGDGMNGSTVEDIARHMNPVERAVLGFMCKEIIDMGRL 433

Query: 421 MWLKECGLETQLVKYVPSNISPENHLLIAKCRNICAA 448
           MWLKECGLETQLVKYVPS+ISPENHLLIAKCRNICAA
Sbjct: 434 MWLKECGLETQLVKYVPSSISPENHLLIAKCRNICAA 470

BLAST of Sed0027463 vs. ExPASy Swiss-Prot
Match: Q10B19 (tRNA:m(4)X modification enzyme TRM13 OS=Oryza sativa subsp. japonica OX=39947 GN=TRM13 PE=1 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 4.3e-64
Identity = 126/239 (52.72%), Postives = 159/239 (66.53%), Query Frame = 0

Query: 4   RCQFWLPKKSRFCSNPPFNDSKFCGNH---SARADG----QWVPCPVDSSHSVLQENLEW 63
           RC FWLP K R C+N P   S++CGNH   SA   G    + VPCPVD SH+VL+ENLE 
Sbjct: 21  RCHFWLPNKRRHCANTPLPTSQYCGNHLPDSASDAGAPFRRLVPCPVDPSHTVLEENLEA 80

Query: 64  HVKRCPLLKQAQSLAVQPFYQKGINAGEEDSTLSNEPASSLSDSISSEMKRNIVHGMSGA 123
           HV +CPL KQA +LA QPFY KGIN+G  +              ++S  KR +VH ++  
Sbjct: 81  HVGKCPLKKQAAALAAQPFYSKGINSGGGEG----------GGGVTSAAKRALVHKLTKD 140

Query: 124 EFHRLLGKIRALHGVICKDIQDSYRIKDACNMWIKGEIDRKIPFQEKHVLQQASILGNME 183
           E   L+ KI+  H      ++DS+ + DAC+ W++ ++DRK+P+QEKHV QQASI+GNME
Sbjct: 141 ELRALIEKIKLAHASAAMAMRDSFLVTDACDNWMRNQVDRKVPYQEKHVTQQASIIGNME 200

Query: 184 EFGVLRNYDGGEQCNV-----VPAVVEFGAGRGYLTQMLADCYGIKRVFLVERKSYKLK 231
            FG+L+   GGE          PAVVEFGAGRGYLTQMLADCYGI+ VFLVER+SYKLK
Sbjct: 201 AFGLLQK--GGEVAEENGVKNAPAVVEFGAGRGYLTQMLADCYGIRNVFLVERRSYKLK 247

BLAST of Sed0027463 vs. ExPASy Swiss-Prot
Match: Q8BYH3 (tRNA:m(4)X modification enzyme TRM13 homolog OS=Mus musculus OX=10090 GN=Trmt13 PE=2 SV=1)

HSP 1 Score: 201.4 bits (511), Expect = 2.1e-50
Identity = 156/481 (32.43%), Postives = 223/481 (46.36%), Query Frame = 0

Query: 4   RCQFWLPKKSRFCSNPPFNDSKFCGNHSARADGQ----WVPCPVDSSHSVLQENLEWHVK 63
           RC +++ KK RFC        +FCG H+  A+ +     + CP+D  H+V ++ L  H+K
Sbjct: 18  RCNYFVEKKKRFCRMVAAAGKRFCGEHAGSAEEENTRKRILCPLDPKHTVYEDQLAKHLK 77

Query: 64  RCPLLKQAQSLAVQPFYQKGINAGEEDSTLSNEPASSLSDSISSEMKRNIVHGMSGAEFH 123
           +C     ++      F+ + INAG +D T   E     S S+S E   N++  +  A   
Sbjct: 78  KC----NSREKPKPDFFIQDINAGLKDETEIPEQLVPFS-SLSEEQLENLIKKLRKAS-- 137

Query: 124 RLLGKIRALHGVICKDIQDSYRIKDACNMWIKGEIDRKIPFQEKHVLQQASILGNMEEFG 183
                   L+      I     + DA N    G+         KH+ QQASILGN+E+  
Sbjct: 138 ------EGLNSTHEDHIMSHPALHDALNDPRNGDC------AVKHLKQQASILGNIEKLK 197

Query: 184 VLRNYDGGEQCNVVPAVVEFGAGRGYLTQ----MLADCYGIKRVFLVERKSYKLKADRSL 243
           +L    G  +C      VEFGAG+G L+      L D   +    LVER + + K D   
Sbjct: 198 LL----GPRRC-----FVEFGAGKGKLSHWVDIALKDAENV-HFILVERVTTRFKVDGKH 257

Query: 244 RQKESLILERLRIDIEDLNLNAVESLR--RIPYIAIGKHLCGPATDLALRCC-------- 303
           R+K+S + ERL+IDI+ L LN V  LR  R+P + IGKHLCG ATD+ALRC         
Sbjct: 258 RKKDS-VFERLQIDIQHLCLNRVPVLREGRLPVVGIGKHLCGVATDVALRCLVETYAASF 317

Query: 304 -------LTKQTNHVNTEQCG----------DSPK----LEGLAIATCCHHLCQWKHYTN 363
                  L K+  +  TE+            D P+    + G+ IA CCHH C W+HY  
Sbjct: 318 EEKDEEPLAKRIKNDKTEKESNTLAKEGSEKDVPETWTPVAGIVIALCCHHRCDWRHYVG 377

Query: 364 KRYLLELGMTKEEFLAITWFTSWAV-DANHSEAISVVTDSKSYLQPSENK-----GDGTN 423
           K Y   LG+   EF      +SWA      S   S VT  +   Q  EN+     GD   
Sbjct: 378 KEYFKALGLGAVEFYYFQRMSSWATCGMRTSLEGSDVTPERKDAQRDENEEHDDGGDRLT 437

Query: 424 ENTVEDIVRNMNPVERAMLGFMCKEIIDMGRLMWLKECGLETQLVKYVPSNISPENHLLI 440
           +   + +   +   E+  +G +CK +ID GRL +L++ G    L  Y    +S EN LL 
Sbjct: 438 DGNTDSLPGILTVEEKKKIGHLCKLLIDQGRLQYLQQKGFSPALQYYTDPLVSLENVLLT 468

BLAST of Sed0027463 vs. ExPASy Swiss-Prot
Match: Q9NUP7 (tRNA:m(4)X modification enzyme TRM13 homolog OS=Homo sapiens OX=9606 GN=TRMT13 PE=1 SV=2)

HSP 1 Score: 190.7 bits (483), Expect = 3.7e-47
Identity = 147/487 (30.18%), Postives = 219/487 (44.97%), Query Frame = 0

Query: 4   RCQFWLPKKSRFCSNPPFNDSKFCGNHSARADGQ----WVPCPVDSSHSVLQENLEWHVK 63
           RC +++ KK RFC        +FCG H+  A+ +     + CP+D  H+V ++ L  H+K
Sbjct: 18  RCGYYVEKKKRFCRMVVAAGKRFCGEHAGAAEEEDARKRILCPLDPKHTVYEDQLAKHLK 77

Query: 64  RCPLLKQAQSLAVQPFYQKGINAGEEDSTLSNEPASSLSDSISSEMKRNIVHGMSGAEFH 123
           +C     ++      FY + INAG  D T   E    +S S+S E    ++  +  A   
Sbjct: 78  KC----NSREKPKPDFYIQDINAGLRDETEIPEQLVPIS-SLSEEQLEKLIKKLRKAS-- 137

Query: 124 RLLGKIRALHGVICKDIQDSYRIKDACNMWIKGEIDRKIPFQEKHVLQQASILGNMEEFG 183
                   L+  +   I     + DA N    G+         KH+ QQASILGN+E   
Sbjct: 138 ------EGLNSTLKDHIMSHPALHDALNDPKNGD------SATKHLKQQASILGNIENLK 197

Query: 184 VLRNYDGGEQCNVVPAVVEFGAGRGYLTQ----MLADCYGIKRVFLVERKSYKLKADRSL 243
           +L    G  +C      VEFGAG+G L+      L D   +    LVE+ + + K D   
Sbjct: 198 LL----GPRRC-----FVEFGAGKGKLSHWVDIALKDAEKV-HFILVEKVTTRFKVDGKH 257

Query: 244 RQKESLILERLRIDIEDLNLNAVESLR--RIPYIAIGKHLCGPATDLALRC--------- 303
           R+K S + ERL+IDI+ L LN +  LR  ++P + IGKHLCG ATDLALRC         
Sbjct: 258 RKKNS-VFERLQIDIQHLCLNKIPVLREEKLPVVGIGKHLCGMATDLALRCLVETYAASF 317

Query: 304 ----------------------CLTKQTNHVNTEQCGDSPKLEGLAIATCCHHLCQWKHY 363
                                  L K+ N  N  +  +   + G+ IA CCHH C W+HY
Sbjct: 318 EERNEEPLAKRIKNDKTEKEIYTLAKEGNEKNVPEKWN--PVAGIVIALCCHHRCDWRHY 377

Query: 364 TNKRYLLELGMTKEEFLAITWFTSWAVDANHSEAISVVTDSKSYLQPSENKGDGTNENT- 423
             K Y   LG+   EF      +SWA       ++     S S  +  +N+ D + E+  
Sbjct: 378 VGKEYFRALGLGAVEFHYFQRMSSWATCGMRKTSLET---SNSTTKRQDNQNDDSEEHDD 437

Query: 424 ---------VEDIVRNMNPVERAMLGFMCKEIIDMGRLMWLKECGLETQLVKYVPSNISP 440
                     + +   ++  E+  +G +CK +ID GR+ +L++ G    L  Y    +S 
Sbjct: 438 GGYRITDDGADCLPGLLSVEEKKKIGHLCKLLIDQGRIQYLQQKGFSPALQYYTDPLVSL 469

BLAST of Sed0027463 vs. ExPASy Swiss-Prot
Match: Q12383 (tRNA:m(4)X modification enzyme TRM13 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TRM13 PE=1 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 1.4e-35
Identity = 129/493 (26.17%), Postives = 219/493 (44.42%), Query Frame = 0

Query: 4   RCQFWLPKKSRFCSNPPFNDSKFCGNH------------------SARADGQWVPCPVDS 63
           +C++++ KK R C     + + +C  H                   A  + + VPCP+D 
Sbjct: 20  QCEYFMEKKKRRCGMTRSSQNLYCSEHLNLMKKAANSQVHNKNGSEAEKERERVPCPLDP 79

Query: 64  SHSVLQENLEWHVKRCPLLKQAQSLAVQPFYQKGINAGEEDSTLSNEPASSLSDSISSEM 123
           +H+V  + L+ H+K+C   K +     +P+Y+ G N   E+  LS    SS+   I++E 
Sbjct: 80  NHTVWADQLKKHLKKCNKTKLSHLNDDKPYYEPGYNG--ENGLLS----SSVKIDITAE- 139

Query: 124 KRNIVHGMSGAEFHRLLGKIRALHGVICKDIQDSYRIKDACNMWIKGEIDRKIPFQEKHV 183
                          L+  I  L+ V   +  D   ++   N  +  +   ++P   KH 
Sbjct: 140 --------------HLVQSIELLYKVFEGESMDELPLRQLNNKLMSLKRFPQLPSNTKHA 199

Query: 184 LQQASILGNMEEFGVLRNYDGGEQCNVVPAVVEFGAGRG------------YLTQMLADC 243
           +QQ+S++ N+ + G    ++  E  N     +EFG GR              LT + A+ 
Sbjct: 200 VQQSSLIENLVDAGA---FERPESLN----FIEFGCGRAEFSRYVSLYLLTQLTSLPAEH 259

Query: 244 YG--IKRVFLVERKSYKLKADRSLRQKESLI-------------LERLRIDIEDLNLNAV 303
            G       L++R + ++K D+ ++   S I             ++R++IDI DL ++ +
Sbjct: 260 SGSNSNEFVLIDRATNRMKFDKKIKDDFSEIKSNSPSKPISCPSIKRIKIDIRDLKMDPI 319

Query: 304 ESLRRIP-----YIAIGKHLCGPATDLALRCCLTKQTNHVNTEQCGDSPKLEGLAIATCC 363
             L+  P     Y+ I KHLCG ATDL LRC       H   +  G +PKL+ + IA CC
Sbjct: 320 --LKSTPGDDIQYVCISKHLCGVATDLTLRCIGNSSILH-GDDNNGCNPKLKAICIAMCC 379

Query: 364 HHLCQWKHYTNKRYLLELG---------MTKEEFL-AITWFTSWAVDANHSEAISVVTDS 423
            H+C +  Y N+ Y+  L          +T E F   +T   SW       +  + +TD 
Sbjct: 380 RHVCDYGDYVNRSYVTSLVEKYRAHGSILTYETFFRVLTKLCSWGTCGR--KPGTAITDI 439

Query: 424 KSYLQPSENKGDGTNENTVEDIVRNMNPVERAMLGFMCKEIIDMGRLMWLKECGLE--TQ 435
            + ++  E    G    T+          ER  +G M + +ID GRL+++KE   E   +
Sbjct: 440 VNVVESFE----GAEPYTI-------TIKERENIGLMARRVIDEGRLVYVKEKFTEFNAE 468

BLAST of Sed0027463 vs. ExPASy Swiss-Prot
Match: A7TSF4 (tRNA:m(4)X modification enzyme TRM13 OS=Vanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294 / BCRC 21397 / CBS 2163 / NBRC 10782 / NRRL Y-8283 / UCD 57-17) OX=436907 GN=TRM13 PE=3 SV=1)

HSP 1 Score: 142.9 bits (359), Expect = 8.8e-33
Identity = 126/469 (26.87%), Postives = 213/469 (45.42%), Query Frame = 0

Query: 4   RCQFWLPKKSRFCSNPPFNDSKFCGNHS-ARADGQWVPCPVDSSHSVLQENLEWHVKRCP 63
           +C+F++ KK R C       SK+C  H     + + +PCP+D +H+V + +L+ H+K+C 
Sbjct: 11  QCEFYMEKKKRRCGMSRNLSSKYCSEHQILNENTKRIPCPLDPNHTVWESDLDSHLKKCN 70

Query: 64  LLKQAQSLAVQPFYQKGINA-GEEDSTLSNEPASSLSDSISSEMKRNIVHGMSGAEFHRL 123
            LK       +P++ K  NA   EDS  +     SL + I   +   ++      +    
Sbjct: 71  KLKLIHQHDNKPYFLKDCNAITTEDSNTTVITKDSLQNWIMKTIP--VLKNFFKDDEKNY 130

Query: 124 LGKIRALHGVICKDIQDSYRIKDA--CNMWIKGEIDRKIPFQEKHVLQQASILGNMEEFG 183
           L  +         DI+ ++ ++D     +     I +K      H +QQ+S++ NM +  
Sbjct: 131 LSNVPL-------DIRKNHTMEDKRFPQLNENDNIGKKY-----HAIQQSSLIQNMIDRN 190

Query: 184 VLRNYDGGEQCNVVPAVVEFGAGRG----YLTQMLADCYGIK----RVFLVERKSYKLKA 243
           +L+  +     N +   +EFG GR     Y+ Q++      K       L++R + ++K 
Sbjct: 191 ILQFKE-----NSLANFIEFGCGRAELSRYVNQVVIKNSEEKIYNPHFILIDRSTNRMKF 250

Query: 244 DRSLRQKESL---------ILERLRIDIEDLNLNAVESLRRIPYIAIGKHLCGPATDLAL 303
           D  ++ K++L          + R+RIDI+DL ++ +   +   YIAI KHLCG ATDL L
Sbjct: 251 DTKMK-KDALEFNQNLNPPTITRIRIDIKDLKIDPLLQ-KDSKYIAISKHLCGVATDLTL 310

Query: 304 RCCLTKQTNHVNTEQCGDSPKLEGLAIATCCHHLCQWKHYTNKRYLLE-LGMTKEE---- 363
           R           T    D+  L+G+ IA CC H+C   +Y N  ++   +G T  E    
Sbjct: 311 RSL---------TYNPDDNDFLQGVCIAMCCRHVCNSNNYVNPDFVKSIIGNTNTEEQLP 370

Query: 364 ----FLAITWFTSWAVDANHSEAISVVTDSKSYLQPSENKGDGTNENTVEDIVRNMNPVE 423
               F A+T   +WA                     S ++   ++EN       N+   E
Sbjct: 371 YDQFFHALTKIAAWAT--------------------SGDRPTSSDENNTH--FTNLPYTE 427

Query: 424 RAMLGFMCKEIIDMGRLMWLKEC---GLETQLVKYVPSNISPENHLLIA 440
           R  LG M + +ID GR  WL++      + +L++YV  +IS EN  L+A
Sbjct: 431 RQELGLMARRVIDQGRCDWLQQKLGESFKVELIRYVEPSISLENVALLA 427

BLAST of Sed0027463 vs. ExPASy TrEMBL
Match: A0A6J1JMC6 (tRNA:m(4)X modification enzyme TRM13 OS=Cucurbita maxima OX=3661 GN=LOC111488184 PE=3 SV=1)

HSP 1 Score: 838.2 bits (2164), Expect = 1.6e-239
Identity = 403/457 (88.18%), Postives = 424/457 (92.78%), Query Frame = 0

Query: 1   MASRCQFWLPKKSRFCSNPPFNDSKFCGNHSARADGQWVPCPVDSSHSVLQENLEWHVKR 60
           MASRCQFWLPKK+RFC+N P NDSKFCGNHS+RAD QWVPCPVD SHSVL+ENLEWHVKR
Sbjct: 1   MASRCQFWLPKKNRFCANSPINDSKFCGNHSSRADTQWVPCPVDPSHSVLRENLEWHVKR 60

Query: 61  CPLLKQAQSLAVQPFYQKGINAGEEDSTLSNEPASSLSDSISSEMKRNIVHGMSGAEFHR 120
           CPLLKQ QSLAVQPFYQKGINAGEEDS  S+E AS LSDSISSEMKRN+V+GMSGAEFHR
Sbjct: 61  CPLLKQTQSLAVQPFYQKGINAGEEDSISSDELASGLSDSISSEMKRNVVYGMSGAEFHR 120

Query: 121 LLGKIRALHGVICKDIQDSYRIKDACNMWIKGEIDRKIPFQEKHVLQQASILGNMEEFGV 180
           LL KIRALHG+ICKDIQDSYRIK+ACNMWIKGEIDRKIPFQEKHVLQQASILGNMEEFGV
Sbjct: 121 LLAKIRALHGLICKDIQDSYRIKEACNMWIKGEIDRKIPFQEKHVLQQASILGNMEEFGV 180

Query: 181 LRNYDGGEQC----------NVVPAVVEFGAGRGYLTQMLADCYGIKRVFLVERKSYKLK 240
           LRNYDGGEQC          NV PAV+EFGAGRGYLTQMLADCYGIKRV LVERKSYKLK
Sbjct: 181 LRNYDGGEQCEGNRSCGNEANVAPAVIEFGAGRGYLTQMLADCYGIKRVLLVERKSYKLK 240

Query: 241 ADRSLRQKESLILERLRIDIEDLNLNAVESLRRIPYIAIGKHLCGPATDLALRCCLTKQT 300
           ADRSLRQKESL+LERLRIDIEDLNLNAVESLR  PY+AIGKHLCGPATDLALRCCLTKQ+
Sbjct: 241 ADRSLRQKESLVLERLRIDIEDLNLNAVESLRHNPYLAIGKHLCGPATDLALRCCLTKQS 300

Query: 301 NHVNTEQCGDSPKLEGLAIATCCHHLCQWKHYTNKRYLLELGMTKEEFLAITWFTSWAVD 360
           NHVNTEQCGD PKL GLAIATCCHHLCQWKHYTNKRYLLELG+TKEEFLAITWFTSWAVD
Sbjct: 301 NHVNTEQCGDRPKLRGLAIATCCHHLCQWKHYTNKRYLLELGITKEEFLAITWFTSWAVD 360

Query: 361 ANHSEAISVVTDSKSYLQPSENKGDGTNENTVEDIVRNMNPVERAMLGFMCKEIIDMGRL 420
           ANHSE IS V DSK++LQ SEN+GDG N +TVEDI R+MNPVERA+LGFMCKEIIDMGRL
Sbjct: 361 ANHSEDISDVADSKTFLQSSENEGDGMNGSTVEDIARHMNPVERAVLGFMCKEIIDMGRL 420

Query: 421 MWLKECGLETQLVKYVPSNISPENHLLIAKCRNICAA 448
           MWLKECGLETQLVKYVPS+ISPENHLLIAKCRNICAA
Sbjct: 421 MWLKECGLETQLVKYVPSSISPENHLLIAKCRNICAA 457

BLAST of Sed0027463 vs. ExPASy TrEMBL
Match: A0A6J1GSU8 (tRNA:m(4)X modification enzyme TRM13 OS=Cucurbita moschata OX=3662 GN=LOC111456639 PE=3 SV=1)

HSP 1 Score: 835.9 bits (2158), Expect = 8.0e-239
Identity = 402/457 (87.96%), Postives = 424/457 (92.78%), Query Frame = 0

Query: 1   MASRCQFWLPKKSRFCSNPPFNDSKFCGNHSARADGQWVPCPVDSSHSVLQENLEWHVKR 60
           MASRCQFWLPKK+RFC+N P NDSKFCGNHS+RAD QWVPCPVD SHSVL+ENLEWHVKR
Sbjct: 1   MASRCQFWLPKKNRFCANSPINDSKFCGNHSSRADTQWVPCPVDPSHSVLRENLEWHVKR 60

Query: 61  CPLLKQAQSLAVQPFYQKGINAGEEDSTLSNEPASSLSDSISSEMKRNIVHGMSGAEFHR 120
           CPLLKQ QSLAVQPFYQKGINAGEEDS  S+E AS LSDSISSEMKRNIV+GMSGAEFHR
Sbjct: 61  CPLLKQTQSLAVQPFYQKGINAGEEDSISSDELASGLSDSISSEMKRNIVYGMSGAEFHR 120

Query: 121 LLGKIRALHGVICKDIQDSYRIKDACNMWIKGEIDRKIPFQEKHVLQQASILGNMEEFGV 180
           LL KIRALHG+ICKDIQDSYRIK+ACNMWIKGEIDRKIPFQEKHVLQQASILGNMEEFGV
Sbjct: 121 LLAKIRALHGLICKDIQDSYRIKEACNMWIKGEIDRKIPFQEKHVLQQASILGNMEEFGV 180

Query: 181 LRNYDGGEQC----------NVVPAVVEFGAGRGYLTQMLADCYGIKRVFLVERKSYKLK 240
           LRNYDGGEQC          NV PAV+EFGAGRGYLTQMLADCYGIKRV LVERKSYKLK
Sbjct: 181 LRNYDGGEQCEGNRSCGNEANVAPAVIEFGAGRGYLTQMLADCYGIKRVLLVERKSYKLK 240

Query: 241 ADRSLRQKESLILERLRIDIEDLNLNAVESLRRIPYIAIGKHLCGPATDLALRCCLTKQT 300
           ADRSLRQKESL+LERLRIDIEDLNLNAVESLR  PY+AIGKHLCGPATDLALRCCLTKQ+
Sbjct: 241 ADRSLRQKESLVLERLRIDIEDLNLNAVESLRHDPYLAIGKHLCGPATDLALRCCLTKQS 300

Query: 301 NHVNTEQCGDSPKLEGLAIATCCHHLCQWKHYTNKRYLLELGMTKEEFLAITWFTSWAVD 360
           NHVNTEQCGD PKL GLAIATCCHHLCQWKHYTNK+YLLELG+ KEEFLAITWFTSWAVD
Sbjct: 301 NHVNTEQCGDRPKLRGLAIATCCHHLCQWKHYTNKKYLLELGIAKEEFLAITWFTSWAVD 360

Query: 361 ANHSEAISVVTDSKSYLQPSENKGDGTNENTVEDIVRNMNPVERAMLGFMCKEIIDMGRL 420
           ANHSE IS V+DSK++LQ SEN+GDG N +TVEDI R+MNPVERA+LGFMCKEIIDMGRL
Sbjct: 361 ANHSEDISDVSDSKTFLQSSENEGDGMNGSTVEDIARHMNPVERAVLGFMCKEIIDMGRL 420

Query: 421 MWLKECGLETQLVKYVPSNISPENHLLIAKCRNICAA 448
           MWLKECGLETQLVKYVPS+ISPENHLLIAKCRNICAA
Sbjct: 421 MWLKECGLETQLVKYVPSSISPENHLLIAKCRNICAA 457

BLAST of Sed0027463 vs. ExPASy TrEMBL
Match: A0A6J1CSZ2 (tRNA:m(4)X modification enzyme TRM13 OS=Momordica charantia OX=3673 GN=LOC111014027 PE=3 SV=1)

HSP 1 Score: 812.8 bits (2098), Expect = 7.3e-232
Identity = 393/456 (86.18%), Postives = 418/456 (91.67%), Query Frame = 0

Query: 1   MASRCQFWLPKKSRFCSNPPFNDSKFCGNHSARADGQWVPCPVDSSHSVLQENLEWHVKR 60
           MASRCQFWLPKK+RFC+N P NDSKFCGNHS+RADGQWVPCPVD SHSVL+ENLEWHVKR
Sbjct: 9   MASRCQFWLPKKNRFCANAPINDSKFCGNHSSRADGQWVPCPVDPSHSVLRENLEWHVKR 68

Query: 61  CPLLKQAQSLAVQPFYQKGINAGEEDSTLSNEPASSLSDSISSEMKRNIVHGMSGAEFHR 120
           CPLLKQAQSLA QPFYQKGINAGEEDS  SNE AS  SDS SSEMKRNIV+GMSGAEFHR
Sbjct: 69  CPLLKQAQSLATQPFYQKGINAGEEDSISSNEQASDFSDSFSSEMKRNIVYGMSGAEFHR 128

Query: 121 LLGKIRALHGVICKDIQDSYRIKDACNMWIKGEIDRKIPFQEKHVLQQASILGNMEEFGV 180
           LLGKIRALHG+ICKDIQDS RIK+AC+MWIKGEIDRKIPFQEKHVLQQASILGN+EEFGV
Sbjct: 129 LLGKIRALHGLICKDIQDSCRIKEACSMWIKGEIDRKIPFQEKHVLQQASILGNLEEFGV 188

Query: 181 LRNYDGGEQC----------NVVPAVVEFGAGRGYLTQMLADCYGIKRVFLVERKSYKLK 240
           LRN DGGEQC          NV PAVVEFGAGRGYLTQ+LADCYGIKRVFLVERKSYKLK
Sbjct: 189 LRNCDGGEQCDSNGSYGNDTNVPPAVVEFGAGRGYLTQILADCYGIKRVFLVERKSYKLK 248

Query: 241 ADRSLRQKESLILERLRIDIEDLNLNAVESLRRIPYIAIGKHLCGPATDLALRCCLTKQT 300
           ADRSLRQKESL+LERLRIDIEDLNLNAVESLR IPY+AIGKHLCGPATDL LRCCL +Q+
Sbjct: 249 ADRSLRQKESLVLERLRIDIEDLNLNAVESLRHIPYLAIGKHLCGPATDLXLRCCLAEQS 308

Query: 301 NHVNTEQCGDSPKLEGLAIATCCHHLCQWKHYTNKRYLLELGMTKEEFLAITWFTSWAVD 360
           N+V+TEQ GD PKL+GLAIATCCHHLCQWKHYTNKRYLLELG+TKEEFLAITWFTSWAVD
Sbjct: 309 NNVHTEQHGDRPKLKGLAIATCCHHLCQWKHYTNKRYLLELGITKEEFLAITWFTSWAVD 368

Query: 361 ANHSEAISVVTDSKSYLQPSENKGDGTNENTVEDIVRNMNPVERAMLGFMCKEIIDMGRL 420
           ANHSE IS VT+ +SYLQPSE +  GTN +TVED+VRNMN VERA+LGFMCKEIIDMGR 
Sbjct: 369 ANHSEDISDVTNPQSYLQPSEKEDGGTNGSTVEDVVRNMNAVERAVLGFMCKEIIDMGRQ 428

Query: 421 MWLKECGLETQLVKYVPSNISPENHLLIAKCRNICA 447
           MWLKECGLETQLVKYVPS+ISPENHLLIAKCRN CA
Sbjct: 429 MWLKECGLETQLVKYVPSSISPENHLLIAKCRNNCA 464

BLAST of Sed0027463 vs. ExPASy TrEMBL
Match: A0A1S3CF14 (tRNA:m(4)X modification enzyme TRM13 OS=Cucumis melo OX=3656 GN=LOC103499984 PE=3 SV=1)

HSP 1 Score: 792.0 bits (2044), Expect = 1.3e-225
Identity = 386/456 (84.65%), Postives = 411/456 (90.13%), Query Frame = 0

Query: 1   MASRCQFWLPKKSRFCSNPPFNDSKFCGNHSARADGQWVPCPVDSSHSVLQENLEWHVKR 60
           MASRCQFWLPKK+RFC+  P NDSKFCGNHS+RADG  VPCPVD SHSVL+ENL+ HVKR
Sbjct: 1   MASRCQFWLPKKNRFCAVAPINDSKFCGNHSSRADGDMVPCPVDPSHSVLRENLQGHVKR 60

Query: 61  CPLLKQAQSLAVQPFYQKGINAGEEDSTLSNEPASSLSDSISSEMKRNIVHGMSGAEFHR 120
           CPLLKQ QSLA QPFYQKGINAGEEDS  +N P S LSDSISSEMKRNIV+GMSG +FHR
Sbjct: 61  CPLLKQTQSLAGQPFYQKGINAGEEDSMSANGPDSGLSDSISSEMKRNIVYGMSGDQFHR 120

Query: 121 LLGKIRALHGVICKDIQDSYRIKDACNMWIKGEIDRKIPFQEKHVLQQASILGNMEEFGV 180
           LLGKIRALH +ICKDIQDSY+IK+ACNMWIKGEIDRKIPFQEKHVLQQASILGN++EFGV
Sbjct: 121 LLGKIRALHDLICKDIQDSYQIKEACNMWIKGEIDRKIPFQEKHVLQQASILGNLDEFGV 180

Query: 181 LRNYDGGEQ----------CNVVPAVVEFGAGRGYLTQMLADCYGIKRVFLVERKSYKLK 240
           LRNYDGG+Q           NVVPAVVEFGAGRGYLTQMLADCYGIKRVFLVERKSYKLK
Sbjct: 181 LRNYDGGQQREGNRSYGNDANVVPAVVEFGAGRGYLTQMLADCYGIKRVFLVERKSYKLK 240

Query: 241 ADRSLRQKESLILERLRIDIEDLNLNAVESLRRIPYIAIGKHLCGPATDLALRCCLTKQT 300
           ADRSLRQKESLILERLRIDIEDLNLNAVESLR  PY+AIGKHLCGPATDLALRCCLTKQ 
Sbjct: 241 ADRSLRQKESLILERLRIDIEDLNLNAVESLRHNPYLAIGKHLCGPATDLALRCCLTKQP 300

Query: 301 NHVNTEQCGDSPKLEGLAIATCCHHLCQWKHYTNKRYLLELGMTKEEFLAITWFTSWAVD 360
           N  +TEQC D  KL GLAIATCCHHLCQWKHYTNKRYLLELG+TKEEFLAITWFTSWAVD
Sbjct: 301 NQADTEQCRDKSKLRGLAIATCCHHLCQWKHYTNKRYLLELGITKEEFLAITWFTSWAVD 360

Query: 361 ANHSEAISVVTDSKSYLQPSENKGDGTNENTVEDIVRNMNPVERAMLGFMCKEIIDMGRL 420
           ANHSE IS VTDSK++LQ SEN+ DG    TV++IV++MNPVERA+LGFMCKEIIDMGRL
Sbjct: 361 ANHSEDISDVTDSKTFLQSSENESDGMIGCTVDEIVKSMNPVERAVLGFMCKEIIDMGRL 420

Query: 421 MWLKECGLETQLVKYVPSNISPENHLLIAKCRNICA 447
           MWLKECGLETQLVKYVPS ISPENHLLIAKCRNICA
Sbjct: 421 MWLKECGLETQLVKYVPSTISPENHLLIAKCRNICA 456

BLAST of Sed0027463 vs. ExPASy TrEMBL
Match: A0A5D3CJH7 (tRNA:m(4)X modification enzyme TRM13 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G00010 PE=3 SV=1)

HSP 1 Score: 789.6 bits (2038), Expect = 6.6e-225
Identity = 385/456 (84.43%), Postives = 410/456 (89.91%), Query Frame = 0

Query: 1   MASRCQFWLPKKSRFCSNPPFNDSKFCGNHSARADGQWVPCPVDSSHSVLQENLEWHVKR 60
           MASRCQFWLPKK+RFC+  P NDSKFCGNHS+RADG  VPCPVD SHSVL+ENL+ HVKR
Sbjct: 1   MASRCQFWLPKKNRFCAVAPINDSKFCGNHSSRADGDMVPCPVDPSHSVLRENLQGHVKR 60

Query: 61  CPLLKQAQSLAVQPFYQKGINAGEEDSTLSNEPASSLSDSISSEMKRNIVHGMSGAEFHR 120
           CPLLKQ QSLA QPFYQKGINAGEED   +N P S LSDSISSEMKRNIV+GMSG +FHR
Sbjct: 61  CPLLKQTQSLAGQPFYQKGINAGEEDLMSANGPDSGLSDSISSEMKRNIVYGMSGDQFHR 120

Query: 121 LLGKIRALHGVICKDIQDSYRIKDACNMWIKGEIDRKIPFQEKHVLQQASILGNMEEFGV 180
           LLGKIRALH +ICKDIQDSY+IK+ACNMWIKGEIDRKIPFQEKHVLQQASILGN++EFGV
Sbjct: 121 LLGKIRALHDLICKDIQDSYQIKEACNMWIKGEIDRKIPFQEKHVLQQASILGNLDEFGV 180

Query: 181 LRNYDGGEQ----------CNVVPAVVEFGAGRGYLTQMLADCYGIKRVFLVERKSYKLK 240
           LRNYDGG+Q           NVVPAVVEFGAGRGYLTQMLADCYGIKRVFLVERKSYKLK
Sbjct: 181 LRNYDGGQQREGNRSYGNDANVVPAVVEFGAGRGYLTQMLADCYGIKRVFLVERKSYKLK 240

Query: 241 ADRSLRQKESLILERLRIDIEDLNLNAVESLRRIPYIAIGKHLCGPATDLALRCCLTKQT 300
           ADRSLRQKESLILERLRIDIEDLNLNAVESLR  PY+AIGKHLCGPATDLALRCCLTKQ 
Sbjct: 241 ADRSLRQKESLILERLRIDIEDLNLNAVESLRHNPYLAIGKHLCGPATDLALRCCLTKQP 300

Query: 301 NHVNTEQCGDSPKLEGLAIATCCHHLCQWKHYTNKRYLLELGMTKEEFLAITWFTSWAVD 360
           N  +TEQC D  KL GLAIATCCHHLCQWKHYTNKRYLLELG+TKEEFLAITWFTSWAVD
Sbjct: 301 NQADTEQCRDKSKLRGLAIATCCHHLCQWKHYTNKRYLLELGITKEEFLAITWFTSWAVD 360

Query: 361 ANHSEAISVVTDSKSYLQPSENKGDGTNENTVEDIVRNMNPVERAMLGFMCKEIIDMGRL 420
           ANHSE IS VTDSK++LQ SEN+ DG    TV++IV++MNPVERA+LGFMCKEIIDMGRL
Sbjct: 361 ANHSEDISDVTDSKTFLQSSENESDGMIGCTVDEIVKSMNPVERAVLGFMCKEIIDMGRL 420

Query: 421 MWLKECGLETQLVKYVPSNISPENHLLIAKCRNICA 447
           MWLKECGLETQLVKYVPS ISPENHLLIAKCRNICA
Sbjct: 421 MWLKECGLETQLVKYVPSTISPENHLLIAKCRNICA 456

BLAST of Sed0027463 vs. TAIR 10
Match: AT4G01880.1 (methyltransferases )

HSP 1 Score: 563.5 bits (1451), Expect = 1.5e-160
Identity = 273/452 (60.40%), Postives = 338/452 (74.78%), Query Frame = 0

Query: 2   ASRCQFWLPKKSRFCSNPPFNDSKFCGNHSARADGQWVPCPVDSSHSVLQENLEWHVKRC 61
           ++RC FWLPKK R C+N     S FCGNHS R +GQW+PCP+D SHSVLQENLEWHVKRC
Sbjct: 6   SNRCSFWLPKKKRSCANTRIESSMFCGNHSQRLEGQWIPCPIDPSHSVLQENLEWHVKRC 65

Query: 62  PLLKQAQSLAVQPFYQKGINAGEEDSTLSNEPASSLSDSISSEMKRNIVHGMSGAEFHRL 121
           PLLKQ   L+ Q FYQKGINAG ++     E        ++SEMKRN+++ MS ++FH+L
Sbjct: 66  PLLKQTVELSGQRFYQKGINAGNDE----EEEKMGSCSLVTSEMKRNLLYSMSVSKFHQL 125

Query: 122 LGKIRALHGVICKDIQDSYRIKDACNMWIKGEIDRKIPFQEKHVLQQASILGNMEEFGVL 181
           + KI  +HG ICKDI+DSY   +ACN+W   EIDRK+PFQEKHVLQQ SILGN+EE GVL
Sbjct: 126 IKKIEDVHGGICKDIEDSYLSPEACNIWFNKEIDRKLPFQEKHVLQQGSILGNLEEIGVL 185

Query: 182 RNYDGGEQC----------NVVPAVVEFGAGRGYLTQMLADCYGIKRVFLVERKSYKLKA 241
           +  +   +C          N  PAVVEFGAGRGYLTQMLADCYGIK+V+LVERKSYKLKA
Sbjct: 186 KRCNINVECCESDLEEKDDNTAPAVVEFGAGRGYLTQMLADCYGIKKVYLVERKSYKLKA 245

Query: 242 DRSLRQKESLILERLRIDIEDLNLNAVESLRRIPYIAIGKHLCGPATDLALRCCLTKQTN 301
           DRSLRQKESL+LER+RIDIEDLNLNAVESL  +PY+A+GKHLCGPATDL+LRCCL++Q  
Sbjct: 246 DRSLRQKESLVLERMRIDIEDLNLNAVESLHGVPYVAVGKHLCGPATDLSLRCCLSRQD- 305

Query: 302 HVNTEQCGDSPKLEGLAIATCCHHLCQWKHYTNKRYLLELGMTKEEFLAITWFTSWAVDA 361
                  G+SP L GLAIATCCHHL QWK Y NK Y+L LG++K+EF  +T FTSWAVD 
Sbjct: 306 -------GESPVLRGLAIATCCHHLSQWKSYINKEYILSLGISKDEFHIMTSFTSWAVDD 365

Query: 362 NHSEAISVVTDSKSYLQPSENKGDGTNEN----TVEDIVRNMNPVERAMLGFMCKEIIDM 421
           +H   +  V D       ++ + +G   +    +VE++V+ M P+ERA+LGF CK+IID 
Sbjct: 366 DHGSKLPGVDDIDLLDSNAKEEEEGEVRDASLISVEEVVKKMKPMERAVLGFKCKQIIDA 425

Query: 422 GRLMWLKECGLETQLVKYVPSNISPENHLLIA 440
           GR+ W+K+ GL ++LVKY+P++ISPEN LLIA
Sbjct: 426 GRMKWVKKHGLNSKLVKYIPASISPENTLLIA 445