Sed0027306 (gene) Chayote v1

BLAST of Sed0027306 vs. NCBI nr
Match: KAG7030564.1 (Gibberellin 2-beta-dioxygenase 8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 626.3 bits (1614), Expect = 1.5e-175
Identity = 298/343 (86.88%), Postives = 319/343 (93.00%), Query Frame = 0

Query: 1   MFKENTAMMNSNPPLFHQYELLLQSTAHLQPFNEGRRSTSSVVEECQLPLIDLRGLESGN 60
           MFKE+T MMNSNPPLF QYELLLQSTAHLQP + G + TSSV+EECQLPL+DL+GLESG 
Sbjct: 1   MFKEDTTMMNSNPPLFQQYELLLQSTAHLQPLHNGYQPTSSVLEECQLPLVDLKGLESGK 60

Query: 61  EKERMACSREIFEASAEWGFFQVTNHGIGTELLSRMSKKQMKLFGAPFEKKSTSGILDNS 120
           EKERMACSREIFEASAEWGFFQV NHGI +ELLSRMSK+QMKLF  PFEKKSTSGILDNS
Sbjct: 61  EKERMACSREIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFEKKSTSGILDNS 120

Query: 121 YRWGAATATHPNQFSWSEAFHIPLTKVSEAACYGEFIYLREVMEELAAAMSRLARSLAGV 180
           YRWGAATAT PNQFSWSEAFHIPLTKVSE  CYG+FI+LREVMEELA AMS+LARSLAGV
Sbjct: 121 YRWGAATATQPNQFSWSEAFHIPLTKVSETDCYGDFIHLREVMEELATAMSKLARSLAGV 180

Query: 181 LVESLGHRKEVFDDICDESTCFLRLNHYPKCPFSGEVSGLVPHTDSDFLTILHQDSGGGL 240
           LVESLGHRKEVF+D+CDESTCFLRLNHYP CPF GEV+GLVPHTDSD+LTILHQDSGGGL
Sbjct: 181 LVESLGHRKEVFEDMCDESTCFLRLNHYPICPFPGEVTGLVPHTDSDYLTILHQDSGGGL 240

Query: 241 QLRKGSQWVAVKPNPQALVVNIGDLLQAWSNDTYKSVKHRVVTNSMRERYSIAYFLCPSY 300
           +L KGSQWVAVKP P+ALVVNIGDLLQAWSNDTYKSVKHRVVTNS+RERYS AYFLCPS 
Sbjct: 241 ELMKGSQWVAVKPIPEALVVNIGDLLQAWSNDTYKSVKHRVVTNSVRERYSTAYFLCPSN 300

Query: 301 HSQIGSCRQPSPYRHFTFGEYRNQIKDDVQKCGYKVGLSRFRL 344
           HS IGSCRQPSPYRHFTFGEYR Q++DDV+K GYKVGLSRFRL
Sbjct: 301 HSGIGSCRQPSPYRHFTFGEYRKQVRDDVEKTGYKVGLSRFRL 343

BLAST of Sed0027306 vs. NCBI nr
Match: XP_022942818.1 (gibberellin 2-beta-dioxygenase 8-like [Cucurbita moschata])

HSP 1 Score: 625.9 bits (1613), Expect = 2.0e-175
Identity = 299/343 (87.17%), Postives = 319/343 (93.00%), Query Frame = 0

Query: 1   MFKENTAMMNSNPPLFHQYELLLQSTAHLQPFNEGRRSTSSVVEECQLPLIDLRGLESGN 60
           MFKE+T MMNSNPPLF QYELLLQSTAHLQP + G + TSSV+EECQLPL+DL+GLESG 
Sbjct: 1   MFKEDTTMMNSNPPLFQQYELLLQSTAHLQPLHNGYQPTSSVLEECQLPLVDLKGLESGK 60

Query: 61  EKERMACSREIFEASAEWGFFQVTNHGIGTELLSRMSKKQMKLFGAPFEKKSTSGILDNS 120
           EKERMACSREIFEASAEWGFFQV NHGI +ELLSRMSK+QMKLF  PFEKKSTSGILDNS
Sbjct: 61  EKERMACSREIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFEKKSTSGILDNS 120

Query: 121 YRWGAATATHPNQFSWSEAFHIPLTKVSEAACYGEFIYLREVMEELAAAMSRLARSLAGV 180
           YRWGAATAT PNQFSWSEAFHIPLTKVSEA CYG+FI+LREVMEELA AMS+LARSLAGV
Sbjct: 121 YRWGAATATQPNQFSWSEAFHIPLTKVSEADCYGDFIHLREVMEELATAMSKLARSLAGV 180

Query: 181 LVESLGHRKEVFDDICDESTCFLRLNHYPKCPFSGEVSGLVPHTDSDFLTILHQDSGGGL 240
           LVESLGHRKEVF+D+CDESTCFLRLNHYP CPF GEV+GLVPHTDSD+LTILHQDSGGGL
Sbjct: 181 LVESLGHRKEVFEDMCDESTCFLRLNHYPICPFPGEVTGLVPHTDSDYLTILHQDSGGGL 240

Query: 241 QLRKGSQWVAVKPNPQALVVNIGDLLQAWSNDTYKSVKHRVVTNSMRERYSIAYFLCPSY 300
           QL KGSQWVAVKP P+ALVVNIGDLLQAWSNDTYKSVKHRVVTNS+RERYS AYFLCPS 
Sbjct: 241 QLMKGSQWVAVKPIPEALVVNIGDLLQAWSNDTYKSVKHRVVTNSVRERYSTAYFLCPSN 300

Query: 301 HSQIGSCRQPSPYRHFTFGEYRNQIKDDVQKCGYKVGLSRFRL 344
            S IGSCRQPSPYRHFTFGEYR Q++DDV+K GYKVGLSRFRL
Sbjct: 301 QSGIGSCRQPSPYRHFTFGEYRKQVRDDVEKTGYKVGLSRFRL 343

BLAST of Sed0027306 vs. NCBI nr
Match: XP_038892146.1 (gibberellin 2-beta-dioxygenase 6-like [Benincasa hispida])

HSP 1 Score: 617.5 bits (1591), Expect = 7.1e-173
Identity = 290/343 (84.55%), Postives = 313/343 (91.25%), Query Frame = 0

Query: 1   MFKENTAMMNSNPPLFHQYELLLQSTAHLQPFNEGRRSTSSVVEECQLPLIDLRGLESGN 60
           MF+E+T MM+S+PPLF QYE LLQSTAHL PFN G   T+SV+EECQLPLIDL+GL+S N
Sbjct: 1   MFEEDTTMMDSDPPLFRQYEFLLQSTAHLLPFNNGNLPTNSVMEECQLPLIDLKGLKSSN 60

Query: 61  EKERMACSREIFEASAEWGFFQVTNHGIGTELLSRMSKKQMKLFGAPFEKKSTSGILDNS 120
           EKER+AC REIFEAS EWGFFQV NHGI TELL+RM+K+QMKLF  PFEKKSTSGILDNS
Sbjct: 61  EKERVACRREIFEASEEWGFFQVINHGIRTELLNRMNKEQMKLFRVPFEKKSTSGILDNS 120

Query: 121 YRWGAATATHPNQFSWSEAFHIPLTKVSEAACYGEFIYLREVMEELAAAMSRLARSLAGV 180
           YRWG  TATHPNQFSWSEAFHIPLTKV+E ACYGEFIYLREVMEELA+AMS+LAR LAGV
Sbjct: 121 YRWGTPTATHPNQFSWSEAFHIPLTKVAEPACYGEFIYLREVMEELASAMSKLARCLAGV 180

Query: 181 LVESLGHRKEVFDDICDESTCFLRLNHYPKCPFSGEVSGLVPHTDSDFLTILHQDSGGGL 240
           LV+SLGHRKE+FDDICDESTCFLRLNHYP CPFSGEVSGLVPHTDSDFLT+LHQD GGGL
Sbjct: 181 LVQSLGHRKELFDDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTVLHQDCGGGL 240

Query: 241 QLRKGSQWVAVKPNPQALVVNIGDLLQAWSNDTYKSVKHRVVTNSMRERYSIAYFLCPSY 300
           QL KGSQWVAVKPNP+ALVVNIGDLLQAWSNDTYKS KHRVV N  RERYSIAYFLCPSY
Sbjct: 241 QLMKGSQWVAVKPNPEALVVNIGDLLQAWSNDTYKSAKHRVVANPKRERYSIAYFLCPSY 300

Query: 301 HSQIGSCRQPSPYRHFTFGEYRNQIKDDVQKCGYKVGLSRFRL 344
           HSQIGSCRQPSPYRHFTF EYR Q++DDV+K GYK+GLS FRL
Sbjct: 301 HSQIGSCRQPSPYRHFTFAEYRKQVRDDVEKTGYKIGLSNFRL 343

BLAST of Sed0027306 vs. NCBI nr
Match: XP_022985325.1 (gibberellin 2-beta-dioxygenase 8-like [Cucurbita maxima])

HSP 1 Score: 617.5 bits (1591), Expect = 7.1e-173
Identity = 295/343 (86.01%), Postives = 314/343 (91.55%), Query Frame = 0

Query: 1   MFKENTAMMNSNPPLFHQYELLLQSTAHLQPFNEGRRSTSSVVEECQLPLIDLRGLESGN 60
           MFKE+T +MNSNPPLFHQY LLLQSTAHLQP   G + TSSV+EECQLPL+DL+GLESG 
Sbjct: 1   MFKEDTTVMNSNPPLFHQYGLLLQSTAHLQPLQSGYQPTSSVLEECQLPLVDLKGLESGK 60

Query: 61  EKERMACSREIFEASAEWGFFQVTNHGIGTELLSRMSKKQMKLFGAPFEKKSTSGILDNS 120
           EKERMACS EIFEASAEWGFFQV NHGI +ELLSRMSK+QMKLF  PFEKKSTSGILDNS
Sbjct: 61  EKERMACSSEIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFEKKSTSGILDNS 120

Query: 121 YRWGAATATHPNQFSWSEAFHIPLTKVSEAACYGEFIYLREVMEELAAAMSRLARSLAGV 180
           YRWGAATAT PNQFSWSEAFHIPLTKVSEA CYG FI+LREVMEELA AMS+LARSLAGV
Sbjct: 121 YRWGAATATQPNQFSWSEAFHIPLTKVSEADCYGNFIHLREVMEELATAMSKLARSLAGV 180

Query: 181 LVESLGHRKEVFDDICDESTCFLRLNHYPKCPFSGEVSGLVPHTDSDFLTILHQDSGGGL 240
           LVESLGHRKEVF+D+CDESTCFLRLNHYP CPF G+V+GLVPHTDSD+LTILHQDSGGGL
Sbjct: 181 LVESLGHRKEVFEDMCDESTCFLRLNHYPICPFPGKVTGLVPHTDSDYLTILHQDSGGGL 240

Query: 241 QLRKGSQWVAVKPNPQALVVNIGDLLQAWSNDTYKSVKHRVVTNSMRERYSIAYFLCPSY 300
           QL KGSQWVAVKP P+ALVVNIGDLLQAWSNDTYKSVKHRVV NS RERYS AYFLCPS 
Sbjct: 241 QLMKGSQWVAVKPIPEALVVNIGDLLQAWSNDTYKSVKHRVVANSARERYSTAYFLCPSD 300

Query: 301 HSQIGSCRQPSPYRHFTFGEYRNQIKDDVQKCGYKVGLSRFRL 344
           HS IGSCRQPSPYRHFTFGEYR Q++DDV+K GYK GLSRFRL
Sbjct: 301 HSGIGSCRQPSPYRHFTFGEYRKQVRDDVEKTGYKEGLSRFRL 343

BLAST of Sed0027306 vs. NCBI nr
Match: XP_023514448.1 (gibberellin 2-beta-dioxygenase 8-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 614.4 bits (1583), Expect = 6.0e-172
Identity = 292/336 (86.90%), Postives = 313/336 (93.15%), Query Frame = 0

Query: 8   MMNSNPPLFHQYELLLQSTAHLQPFNEGRRSTSSVVEECQLPLIDLRGLESGNEKERMAC 67
           MMNSNPPLF QYELLLQSTAHLQP + G + TSSV+EECQLPL+DL+GLESG EKERMAC
Sbjct: 1   MMNSNPPLFQQYELLLQSTAHLQPLHNGYQPTSSVLEECQLPLVDLKGLESGKEKERMAC 60

Query: 68  SREIFEASAEWGFFQVTNHGIGTELLSRMSKKQMKLFGAPFEKKSTSGILDNSYRWGAAT 127
           SREIFEASAEWGFFQV NHGI +ELLSRMSK+QMKLF  PFE KSTSGILDNSYRWGAAT
Sbjct: 61  SREIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFENKSTSGILDNSYRWGAAT 120

Query: 128 ATHPNQFSWSEAFHIPLTKVSEAACYGEFIYLREVMEELAAAMSRLARSLAGVLVESLGH 187
           AT PNQFSWSEAFHIPLTKVSEA CYG+FI+LREVMEELA AMS+LARSLAGVLVESLGH
Sbjct: 121 ATQPNQFSWSEAFHIPLTKVSEADCYGDFIHLREVMEELATAMSKLARSLAGVLVESLGH 180

Query: 188 RKEVFDDICDESTCFLRLNHYPKCPFSGEVSGLVPHTDSDFLTILHQDSGGGLQLRKGSQ 247
           RKEVF+D+CDESTCFLRLNHYP CPF GEV+GLVPHTDSD+LTILHQD+GGGL+L KGSQ
Sbjct: 181 RKEVFEDMCDESTCFLRLNHYPICPFPGEVTGLVPHTDSDYLTILHQDAGGGLELMKGSQ 240

Query: 248 WVAVKPNPQALVVNIGDLLQAWSNDTYKSVKHRVVTNSMRERYSIAYFLCPSYHSQIGSC 307
           WVAVKP P+ALVVNIGDLLQAWSNDTYKSVKHRVVTNS+RERYS AYFLCPS HS IGSC
Sbjct: 241 WVAVKPIPEALVVNIGDLLQAWSNDTYKSVKHRVVTNSVRERYSTAYFLCPSNHSGIGSC 300

Query: 308 RQPSPYRHFTFGEYRNQIKDDVQKCGYKVGLSRFRL 344
           RQPSPYRHFTFGEYR Q++DDV+K GYKVGLSRFRL
Sbjct: 301 RQPSPYRHFTFGEYRKQVRDDVEKTGYKVGLSRFRL 336

BLAST of Sed0027306 vs. ExPASy Swiss-Prot
Match: Q7XP65 (Gibberellin 2-beta-dioxygenase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2OX6 PE=1 SV=1)

HSP 1 Score: 357.8 bits (917), Expect = 1.3e-97
Identity = 170/311 (54.66%), Postives = 216/311 (69.45%), Query Frame = 0

Query: 39  TSSVVEECQLPLIDLRGLESGNEKERMACSREIFEASAEWGFFQVTNHGIGTELLSRMSK 98
           +   V E  LP++DL  L  G   ER AC+  +  A++EWGFFQ+TNHG+G EL+  M +
Sbjct: 41  SGGAVLERDLPMVDLERLTRGGAGERKACAGAMARAASEWGFFQLTNHGVGRELMEEMRR 100

Query: 99  KQMKLFGAPFEKKSTSGILDNSYRWGAATATHPNQFSWSEAFHIPLTKVSEAAC-YGEFI 158
           +Q +LF  PFE K  +G+L+ SYRWG  TAT     SWSEAFH+PL  +S A C +G+  
Sbjct: 101 EQARLFRLPFETKEKAGLLNGSYRWGNPTATSLRHLSWSEAFHVPLASISGADCDFGDLT 160

Query: 159 YLREVMEELAAAMSRLARSLAGVLVESLGHR-------KEVFDDICDESTCFLRLNHYPK 218
            LR VM+E+A AMSR+A ++A  L E L  R          F   CDE+TCFLRLN YP 
Sbjct: 161 SLRGVMQEVAEAMSRVANTVAAALAEELTGRGGGGASAAPWFPAGCDETTCFLRLNRYPA 220

Query: 219 CPFSGEVSGLVPHTDSDFLTILHQDSGGGLQLRKGSQWVAVKPNPQALVVNIGDLLQAWS 278
           CPF+ +  GLVPHTDSDFLT+L QD  GGL L K S+WVAV+P P ALVVNIGDL QAWS
Sbjct: 221 CPFAADTFGLVPHTDSDFLTVLCQDQVGGLHLMKDSRWVAVRPRPDALVVNIGDLFQAWS 280

Query: 279 NDTYKSVKHRVVTNSMRERYSIAYFLCPSYHSQIGSCRQPSPYRHFTFGEYRNQIKDDVQ 338
           N+ YKSV+H+VV N+  +R S+AYFLCPSY S +G+C +PSPYR FTFGEYR ++++DV+
Sbjct: 281 NNRYKSVEHKVVANAKTDRLSVAYFLCPSYDSLVGTCGEPSPYRAFTFGEYRKKVQEDVR 340

Query: 339 KCGYKVGLSRF 342
             G K+GL  F
Sbjct: 341 TTGKKIGLPNF 351

BLAST of Sed0027306 vs. ExPASy Swiss-Prot
Match: O49561 (Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana OX=3702 GN=GA2OX8 PE=1 SV=2)

HSP 1 Score: 330.5 bits (846), Expect = 2.3e-89
Identity = 160/305 (52.46%), Postives = 208/305 (68.20%), Query Frame = 0

Query: 38  STSSVVEECQLPLIDLRGLESGNEKERMACSREIFEASAEWGFFQVTNHGIGTELLSRMS 97
           S ++VVEE +LP+ID+  L  G E+ER  C   I  AS EWGFFQV NHGI  ++L +M 
Sbjct: 32  SVTAVVEEVELPVIDVSRLIDGAEEEREKCKEAIARASREWGFFQVINHGISMDVLEKMR 91

Query: 98  KKQMKLFGAPFEKKSTSGILD-NSYRWGAATATHPNQFSWSEAFHIPLTKVSEAACYGEF 157
           ++Q+++F  PF+KKS S      SYRWG  +AT   Q SWSEAFH+P+T +S+     +F
Sbjct: 92  QEQIRVFREPFDKKSKSEKFSAGSYRWGTPSATSIRQLSWSEAFHVPMTDISDNK---DF 151

Query: 158 IYLREVMEELAAAMSRLARSLAGVLVESLGHRKEVFDDICDESTCFLRLNHYPKCPFSGE 217
             L   ME+ A+    LA  LA VL E  G     F + C  +TC+LR+N YP CP   E
Sbjct: 152 TTLSSTMEKFASESEALAYMLAEVLAEKSGQNSSFFKENCVRNTCYLRMNRYPPCPKPSE 211

Query: 218 VSGLVPHTDSDFLTILHQDSGGGLQLRKGSQWVAVKPNPQALVVNIGDLLQAWSNDTYKS 277
           V GL+PHTDSDFLTIL+QD  GGLQL K ++W+AVKPNP+AL++NIGDL QAWSN  YKS
Sbjct: 212 VYGLMPHTDSDFLTILYQDQVGGLQLIKDNRWIAVKPNPKALIINIGDLFQAWSNGMYKS 271

Query: 278 VKHRVVTNSMRERYSIAYFLCPSYHSQIGSCRQPSPYRHFTFGEYRNQIKDDVQKCGYKV 337
           V+HRV+TN   ER+S AYF+CPSY + I        YR+F+F E+R Q+++DV+K G+KV
Sbjct: 272 VEHRVMTNPKVERFSTAYFMCPSYDAVIECSSDRPAYRNFSFREFRQQVQEDVKKFGFKV 331

Query: 338 GLSRF 342
           GL RF
Sbjct: 332 GLPRF 333

BLAST of Sed0027306 vs. ExPASy Swiss-Prot
Match: Q8LGZ9 (Gibberellin 2-beta-dioxygenase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2OX5 PE=1 SV=1)

HSP 1 Score: 298.9 bits (764), Expect = 7.4e-80
Identity = 165/344 (47.97%), Postives = 210/344 (61.05%), Query Frame = 0

Query: 10  NSNPPLFHQYELLLQSTAHLQPFNEGRRSTSSVVEECQLPLIDLRGLESGNEKERMACSR 69
           NSNPPL   Y+ L     H    + G    +  V+EC+LP+IDL GL    +     C  
Sbjct: 9   NSNPPLMSTYKHLFVE-QHRLDMDMG----AIDVDECELPVIDLAGLMEAEQ----VCRA 68

Query: 70  EIFEASAEWGFFQVTNHGIGTELLSRMSKKQMKLFGAPFEKKSTS---GILDNSYRWGAA 129
           ++  A++EWGFFQVTNHG+   LL  +   Q+ +F  PF++K T    G    SYRWG  
Sbjct: 69  DMVRAASEWGFFQVTNHGVPQALLRELHDAQVAVFRRPFQEKVTERLLGFSPESYRWGTP 128

Query: 130 TATHPNQFSWSEAFHIPLTKVSEAACYGEFIYLREVMEELAAAMSRLARSLAGVLVESLG 189
           TA    Q SWSEA+HIP+T    +      I  R V+EE++ AM  LA+ LA +L+  L 
Sbjct: 129 TAKCLEQLSWSEAYHIPMTTPRPSTS----IRARAVIEEVSRAMYELAQKLAEILMRGLP 188

Query: 190 HRKEVFDDICD-ESTCFLRLNHYPKCPFSGEVSGLVPHTDSDFLTILH--QDSGGGLQLR 249
              E    +   E TCFLRLN YP C  +    GL PHTDSD LTI+H  QD+ GGLQL 
Sbjct: 189 GAGEGETMVTTREETCFLRLNRYPPCAMAMGGFGLCPHTDSDLLTIVHQQQDTVGGLQLL 248

Query: 250 KGSQWVAVKPNPQALVVNIGDLLQAWSNDTYKSVKHRVVTNSMRERYSIAYFLCPSYHSQ 309
           KG +WVAVKP+P  L+VN+GDLLQAWSND YKSV+HRV+ N+  ER+S+A+FLCPSYH+ 
Sbjct: 249 KGGRWVAVKPSPSTLIVNVGDLLQAWSNDVYKSVEHRVMANATLERFSMAFFLCPSYHTL 308

Query: 310 IGSCR-----QPSPYRHFTFGEYRNQIKDDVQKCGYKVGLSRFR 343
           I           + YR FTFGEYR QI +DV+  G K+GL RFR
Sbjct: 309 IIPSSSHVHDDDAHYRSFTFGEYRKQIMEDVRSTGRKIGLHRFR 339

BLAST of Sed0027306 vs. ExPASy Swiss-Prot
Match: Q9C6I4 (Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana OX=3702 GN=GA2OX7 PE=1 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 7.6e-69
Identity = 137/313 (43.77%), Postives = 195/313 (62.30%), Query Frame = 0

Query: 33  NEGRRSTSSV-VEECQLPLIDLRGLESGNEKERMACSREIFEASAEWGFFQVTNHGIGTE 92
           +E   +TS++     +LP+IDL  L SG E +R  C +++  A+ EWGFFQ+ NHGI  +
Sbjct: 24  SESNTNTSTIQTSGIKLPVIDLSHLTSGEEVKRKRCVKQMVAAAKEWGFFQIVNHGIPKD 83

Query: 93  LLSRMSKKQMKLFGAPFE---KKSTSGILDNSYRWGAATATHPNQFSWSEAFHIPLTKVS 152
           +   M  ++ KLF  PF    ++  S +  NSYRWG  +AT P Q+S SEAFHI L++VS
Sbjct: 84  VFEMMLLEEKKLFDQPFSVKVRERFSDLSKNSYRWGNPSATSPAQYSVSEAFHIILSEVS 143

Query: 153 EAACYGEFIYLREVMEELAAAMSRLARSLAGVLVESLGHRKEVFDDICDESTCFLRLNHY 212
             +   +   LR ++E     ++R+A+ +  +L + +    E F++I +    FLRLN Y
Sbjct: 144 RIS--DDRNNLRTIVETYVQEIARVAQMICEILGKQVNVSSEYFENIFELENSFLRLNKY 203

Query: 213 PKCPFSGEVSGLVPHTDSDFLTILHQDSGGGLQLRKGSQWVAVKPNPQALVVNIGDLLQA 272
               F  EV GLVPHTD+ FLTIL QD  GGL+L    QW++VKP  +AL VNIGD+ QA
Sbjct: 204 HPSVFGSEVFGLVPHTDTSFLTILSQDQIGGLELENNGQWISVKPCLEALTVNIGDMFQA 263

Query: 273 WSNDTYKSVKHRVVTNSMRERYSIAYFLCPSYHSQIGSCRQPSPYRHFTFGEYRNQIKDD 332
            SN  Y+SV+HRV++ +  ER SIA+F+CP   ++I     P  YR F+F EY+ Q + D
Sbjct: 264 LSNGVYQSVRHRVISPANIERMSIAFFVCPYLETEIDCFGYPKKYRRFSFREYKEQSEHD 323

Query: 333 VQKCGYKVGLSRF 342
           V++ G KVGLSRF
Sbjct: 324 VKETGDKVGLSRF 334

BLAST of Sed0027306 vs. ExPASy Swiss-Prot
Match: Q9FFF6 (Jasmonate-induced oxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=JOX2 PE=1 SV=1)

HSP 1 Score: 161.0 bits (406), Expect = 2.4e-38
Identity = 102/309 (33.01%), Postives = 152/309 (49.19%), Query Frame = 0

Query: 28  HLQPFNEGRRSTSSVVEECQLPLIDLRGLESGNEKERMACSREIFEASAEWGFFQVTNHG 87
           +++P +    +T        +P+IDL GL S            I EA   WGFFQV NHG
Sbjct: 42  YIKPASLRPTTTEDAPTATNIPIIDLEGLFSEEGLSDDVIMARISEACRGWGFFQVVNHG 101

Query: 88  IGTELLSRMSKKQMKLFGAPFEKKSTSGILDNSYR-WGAATATHPN-QFSWSEAF--HIP 147
           +  EL+    +   + F  P   K T      +Y  +G+           WS+ +  H+ 
Sbjct: 102 VKPELMDAARENWREFFHMPVNAKETYSNSPRTYEGYGSRLGVEKGASLDWSDYYFLHLL 161

Query: 148 LTKVSEAACYGEF-IYLREVMEELAAAMSRLARSLAGVLVESLGHRKEVFDDIC--DEST 207
              + +   +  F   +REV++E    + +L+  +  VL  +LG +++ F +    +   
Sbjct: 162 PHHLKDFNKWPSFPPTIREVIDEYGEELVKLSGRIMRVLSTNLGLKEDKFQEAFGGENIG 221

Query: 208 CFLRLNHYPKCPFSGEVSGLVPHTDSDFLTI-LHQDSGGGLQLRKGSQWVAVKPNPQALV 267
             LR+N+YPKCP      GL PH+D   +TI L  D   GLQ+RK   W+ VKP+P A +
Sbjct: 222 ACLRVNYYPKCPRPELALGLSPHSDPGGMTILLPDDQVFGLQVRKDDTWITVKPHPHAFI 281

Query: 268 VNIGDLLQAWSNDTYKSVKHRVVTNSMRERYSIAYFLCPSYH------SQIGSCRQPSPY 323
           VNIGD +Q  SN TYKSV+HRV+ NS +ER S+A+F  P          ++ S   P  Y
Sbjct: 282 VNIGDQIQILSNSTYKSVEHRVIVNSDKERVSLAFFYNPKSDIPIQPLQELVSTHNPPLY 341

BLAST of Sed0027306 vs. ExPASy TrEMBL
Match: A0A6J1FVR3 (gibberellin 2-beta-dioxygenase 8-like OS=Cucurbita moschata OX=3662 GN=LOC111447734 PE=3 SV=1)

HSP 1 Score: 625.9 bits (1613), Expect = 9.7e-176
Identity = 299/343 (87.17%), Postives = 319/343 (93.00%), Query Frame = 0

Query: 1   MFKENTAMMNSNPPLFHQYELLLQSTAHLQPFNEGRRSTSSVVEECQLPLIDLRGLESGN 60
           MFKE+T MMNSNPPLF QYELLLQSTAHLQP + G + TSSV+EECQLPL+DL+GLESG 
Sbjct: 1   MFKEDTTMMNSNPPLFQQYELLLQSTAHLQPLHNGYQPTSSVLEECQLPLVDLKGLESGK 60

Query: 61  EKERMACSREIFEASAEWGFFQVTNHGIGTELLSRMSKKQMKLFGAPFEKKSTSGILDNS 120
           EKERMACSREIFEASAEWGFFQV NHGI +ELLSRMSK+QMKLF  PFEKKSTSGILDNS
Sbjct: 61  EKERMACSREIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFEKKSTSGILDNS 120

Query: 121 YRWGAATATHPNQFSWSEAFHIPLTKVSEAACYGEFIYLREVMEELAAAMSRLARSLAGV 180
           YRWGAATAT PNQFSWSEAFHIPLTKVSEA CYG+FI+LREVMEELA AMS+LARSLAGV
Sbjct: 121 YRWGAATATQPNQFSWSEAFHIPLTKVSEADCYGDFIHLREVMEELATAMSKLARSLAGV 180

Query: 181 LVESLGHRKEVFDDICDESTCFLRLNHYPKCPFSGEVSGLVPHTDSDFLTILHQDSGGGL 240
           LVESLGHRKEVF+D+CDESTCFLRLNHYP CPF GEV+GLVPHTDSD+LTILHQDSGGGL
Sbjct: 181 LVESLGHRKEVFEDMCDESTCFLRLNHYPICPFPGEVTGLVPHTDSDYLTILHQDSGGGL 240

Query: 241 QLRKGSQWVAVKPNPQALVVNIGDLLQAWSNDTYKSVKHRVVTNSMRERYSIAYFLCPSY 300
           QL KGSQWVAVKP P+ALVVNIGDLLQAWSNDTYKSVKHRVVTNS+RERYS AYFLCPS 
Sbjct: 241 QLMKGSQWVAVKPIPEALVVNIGDLLQAWSNDTYKSVKHRVVTNSVRERYSTAYFLCPSN 300

Query: 301 HSQIGSCRQPSPYRHFTFGEYRNQIKDDVQKCGYKVGLSRFRL 344
            S IGSCRQPSPYRHFTFGEYR Q++DDV+K GYKVGLSRFRL
Sbjct: 301 QSGIGSCRQPSPYRHFTFGEYRKQVRDDVEKTGYKVGLSRFRL 343

BLAST of Sed0027306 vs. ExPASy TrEMBL
Match: A0A6J1JB12 (gibberellin 2-beta-dioxygenase 8-like OS=Cucurbita maxima OX=3661 GN=LOC111483364 PE=3 SV=1)

HSP 1 Score: 617.5 bits (1591), Expect = 3.4e-173
Identity = 295/343 (86.01%), Postives = 314/343 (91.55%), Query Frame = 0

Query: 1   MFKENTAMMNSNPPLFHQYELLLQSTAHLQPFNEGRRSTSSVVEECQLPLIDLRGLESGN 60
           MFKE+T +MNSNPPLFHQY LLLQSTAHLQP   G + TSSV+EECQLPL+DL+GLESG 
Sbjct: 1   MFKEDTTVMNSNPPLFHQYGLLLQSTAHLQPLQSGYQPTSSVLEECQLPLVDLKGLESGK 60

Query: 61  EKERMACSREIFEASAEWGFFQVTNHGIGTELLSRMSKKQMKLFGAPFEKKSTSGILDNS 120
           EKERMACS EIFEASAEWGFFQV NHGI +ELLSRMSK+QMKLF  PFEKKSTSGILDNS
Sbjct: 61  EKERMACSSEIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFEKKSTSGILDNS 120

Query: 121 YRWGAATATHPNQFSWSEAFHIPLTKVSEAACYGEFIYLREVMEELAAAMSRLARSLAGV 180
           YRWGAATAT PNQFSWSEAFHIPLTKVSEA CYG FI+LREVMEELA AMS+LARSLAGV
Sbjct: 121 YRWGAATATQPNQFSWSEAFHIPLTKVSEADCYGNFIHLREVMEELATAMSKLARSLAGV 180

Query: 181 LVESLGHRKEVFDDICDESTCFLRLNHYPKCPFSGEVSGLVPHTDSDFLTILHQDSGGGL 240
           LVESLGHRKEVF+D+CDESTCFLRLNHYP CPF G+V+GLVPHTDSD+LTILHQDSGGGL
Sbjct: 181 LVESLGHRKEVFEDMCDESTCFLRLNHYPICPFPGKVTGLVPHTDSDYLTILHQDSGGGL 240

Query: 241 QLRKGSQWVAVKPNPQALVVNIGDLLQAWSNDTYKSVKHRVVTNSMRERYSIAYFLCPSY 300
           QL KGSQWVAVKP P+ALVVNIGDLLQAWSNDTYKSVKHRVV NS RERYS AYFLCPS 
Sbjct: 241 QLMKGSQWVAVKPIPEALVVNIGDLLQAWSNDTYKSVKHRVVANSARERYSTAYFLCPSD 300

Query: 301 HSQIGSCRQPSPYRHFTFGEYRNQIKDDVQKCGYKVGLSRFRL 344
           HS IGSCRQPSPYRHFTFGEYR Q++DDV+K GYK GLSRFRL
Sbjct: 301 HSGIGSCRQPSPYRHFTFGEYRKQVRDDVEKTGYKEGLSRFRL 343

BLAST of Sed0027306 vs. ExPASy TrEMBL
Match: A0A0A0KIW0 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G052540 PE=3 SV=1)

HSP 1 Score: 613.6 bits (1581), Expect = 5.0e-172
Identity = 289/344 (84.01%), Postives = 315/344 (91.57%), Query Frame = 0

Query: 1   MFKENTA-MMNSNPPLFHQYELLLQSTAHLQPFNEGRRSTSSVVEECQLPLIDLRGLESG 60
           MFKENT  MM+SNPPLF QYE LLQSTAHLQPFN G   T+SV+EECQLPLIDL+GL+S 
Sbjct: 1   MFKENTTMMMDSNPPLFDQYEFLLQSTAHLQPFNNGNLPTNSVMEECQLPLIDLKGLKSS 60

Query: 61  NEKERMACSREIFEASAEWGFFQVTNHGIGTELLSRMSKKQMKLFGAPFEKKSTSGILDN 120
           +EKER+AC REIFEAS EWGFFQV NHGI TELL+RM+K+Q+KLFG PFEKK TSGILDN
Sbjct: 61  DEKERVACRREIFEASEEWGFFQVINHGIHTELLNRMNKEQIKLFGVPFEKKFTSGILDN 120

Query: 121 SYRWGAATATHPNQFSWSEAFHIPLTKVSEAACYGEFIYLREVMEELAAAMSRLARSLAG 180
           SYRWG  TATHPNQFSWSEAFHIPLTKVSEAACYG+FIYLREVMEE+A+AMS++AR LAG
Sbjct: 121 SYRWGTPTATHPNQFSWSEAFHIPLTKVSEAACYGDFIYLREVMEEVASAMSKVARKLAG 180

Query: 181 VLVESLGHRKEVFDDICDESTCFLRLNHYPKCPFSGEVSGLVPHTDSDFLTILHQDSGGG 240
           VLVES+G RKE+ +DICDESTCFLRLNHYP CPFSGEVSGLVPHTDSDFLTILHQDSGGG
Sbjct: 181 VLVESMGQRKELLEDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTILHQDSGGG 240

Query: 241 LQLRKGSQWVAVKPNPQALVVNIGDLLQAWSNDTYKSVKHRVVTNSMRERYSIAYFLCPS 300
           LQ+ KGSQW+AVKPNPQALVVNIGDLLQAWSNDTYKS KHRVV N MRERYSIAYFLCPS
Sbjct: 241 LQVMKGSQWLAVKPNPQALVVNIGDLLQAWSNDTYKSAKHRVVANPMRERYSIAYFLCPS 300

Query: 301 YHSQIGSCRQPSPYRHFTFGEYRNQIKDDVQKCGYKVGLSRFRL 344
           YHSQIGSCR+PSPYRHFTF EYR Q++DDV+K GYK+GLS FRL
Sbjct: 301 YHSQIGSCREPSPYRHFTFAEYRKQVRDDVEKTGYKIGLSNFRL 344

BLAST of Sed0027306 vs. ExPASy TrEMBL
Match: A0A6J1FTS8 (gibberellin 2-beta-dioxygenase 8-like OS=Cucurbita moschata OX=3662 GN=LOC111447241 PE=3 SV=1)

HSP 1 Score: 610.9 bits (1574), Expect = 3.2e-171
Identity = 291/340 (85.59%), Postives = 307/340 (90.29%), Query Frame = 0

Query: 4   ENTAMMNSNPPLFHQYELLLQSTAHLQPFNEGRRSTSSVVEECQLPLIDLRGLESGNEKE 63
           ENT M +SNPPLFHQYELLLQS A LQ  N G +  +SV+EECQLPLIDL GLES NEKE
Sbjct: 37  ENTTMTDSNPPLFHQYELLLQSKAQLQLLNNGNQQINSVMEECQLPLIDLTGLESSNEKE 96

Query: 64  RMACSREIFEASAEWGFFQVTNHGIGTELLSRMSKKQMKLFGAPFEKKSTSGILDNSYRW 123
           R ACSREIF ASAEWGFFQV NHGI TELLSRMSK+QMKLFG PFEKKSTSGILD+SYRW
Sbjct: 97  RRACSREIFGASAEWGFFQVINHGIRTELLSRMSKEQMKLFGVPFEKKSTSGILDHSYRW 156

Query: 124 GAATATHPNQFSWSEAFHIPLTKVSEAACYGEFIYLREVMEELAAAMSRLARSLAGVLVE 183
           G  TATHPNQFSWSEAFHIPLTKVSEAACYGEFI LREVMEELA AMS+LARSLAGVLVE
Sbjct: 157 GTPTATHPNQFSWSEAFHIPLTKVSEAACYGEFINLREVMEELATAMSKLARSLAGVLVE 216

Query: 184 SLGHRKEVFDDICDESTCFLRLNHYPKCPFSGEVSGLVPHTDSDFLTILHQDSGGGLQLR 243
            LGHRKE+FDDICDESTCFLRLNHYP CPF G+VSGLVPHTDSDFLTILHQD  GGLQL 
Sbjct: 217 CLGHRKEIFDDICDESTCFLRLNHYPICPFPGDVSGLVPHTDSDFLTILHQDRSGGLQLM 276

Query: 244 KGSQWVAVKPNPQALVVNIGDLLQAWSNDTYKSVKHRVVTNSMRERYSIAYFLCPSYHSQ 303
           KGSQWVAVKPNP+ALVVNIGDLLQAWSNDTYKSVKH VV NSMRERYSIAYFLCPSYHS 
Sbjct: 277 KGSQWVAVKPNPEALVVNIGDLLQAWSNDTYKSVKHMVVANSMRERYSIAYFLCPSYHSP 336

Query: 304 IGSCRQPSPYRHFTFGEYRNQIKDDVQKCGYKVGLSRFRL 344
           IGSC+QPSPYRHFTF EYR Q++DDV+K GYK+GLS+FRL
Sbjct: 337 IGSCKQPSPYRHFTFAEYRKQVRDDVEKIGYKIGLSKFRL 376

BLAST of Sed0027306 vs. ExPASy TrEMBL
Match: A0A6J1JNB9 (gibberellin 2-beta-dioxygenase 8-like OS=Cucurbita maxima OX=3661 GN=LOC111486865 PE=3 SV=1)

HSP 1 Score: 608.2 bits (1567), Expect = 2.1e-170
Identity = 290/340 (85.29%), Postives = 305/340 (89.71%), Query Frame = 0

Query: 4   ENTAMMNSNPPLFHQYELLLQSTAHLQPFNEGRRSTSSVVEECQLPLIDLRGLESGNEKE 63
           ENT M +SNPPLFHQYELLLQS A LQ  N G +  +SV+ ECQLPLIDL GLES NEKE
Sbjct: 37  ENTTMTDSNPPLFHQYELLLQSKAQLQLLNNGNQQINSVMGECQLPLIDLTGLESSNEKE 96

Query: 64  RMACSREIFEASAEWGFFQVTNHGIGTELLSRMSKKQMKLFGAPFEKKSTSGILDNSYRW 123
           R ACSREIF AS EWGFFQV NHGI TELLSRMSK+QMKLFG PFEKKSTSGILD+SYRW
Sbjct: 97  RRACSREIFGASTEWGFFQVINHGIRTELLSRMSKEQMKLFGVPFEKKSTSGILDHSYRW 156

Query: 124 GAATATHPNQFSWSEAFHIPLTKVSEAACYGEFIYLREVMEELAAAMSRLARSLAGVLVE 183
           G  TATHPNQFSWSEAFHIPLTKVSEAACYGEFI LREVMEELA AMS+LARSLAGVLVE
Sbjct: 157 GTPTATHPNQFSWSEAFHIPLTKVSEAACYGEFINLREVMEELATAMSKLARSLAGVLVE 216

Query: 184 SLGHRKEVFDDICDESTCFLRLNHYPKCPFSGEVSGLVPHTDSDFLTILHQDSGGGLQLR 243
            LGHRKE+FDDICDESTCFLRLNHYP CPF G+VSGLVPHTDSDFLTILHQD  GGLQL 
Sbjct: 217 CLGHRKEIFDDICDESTCFLRLNHYPICPFPGDVSGLVPHTDSDFLTILHQDRSGGLQLM 276

Query: 244 KGSQWVAVKPNPQALVVNIGDLLQAWSNDTYKSVKHRVVTNSMRERYSIAYFLCPSYHSQ 303
           KGSQWVAV PNP+ALVVNIGDLLQAWSNDTYKSVKHRVV NSMRERYSIAYFLCPSYHS 
Sbjct: 277 KGSQWVAVNPNPEALVVNIGDLLQAWSNDTYKSVKHRVVANSMRERYSIAYFLCPSYHSP 336

Query: 304 IGSCRQPSPYRHFTFGEYRNQIKDDVQKCGYKVGLSRFRL 344
           IGSCRQPSPYRHFTF EYR Q++DDV+K GYK+GLS+FRL
Sbjct: 337 IGSCRQPSPYRHFTFAEYRKQVRDDVEKIGYKIGLSKFRL 376

BLAST of Sed0027306 vs. TAIR 10
Match: AT4G21200.1 (gibberellin 2-oxidase 8 )

HSP 1 Score: 330.5 bits (846), Expect = 1.6e-90
Identity = 160/305 (52.46%), Postives = 208/305 (68.20%), Query Frame = 0

Query: 38  STSSVVEECQLPLIDLRGLESGNEKERMACSREIFEASAEWGFFQVTNHGIGTELLSRMS 97
           S ++VVEE +LP+ID+  L  G E+ER  C   I  AS EWGFFQV NHGI  ++L +M 
Sbjct: 32  SVTAVVEEVELPVIDVSRLIDGAEEEREKCKEAIARASREWGFFQVINHGISMDVLEKMR 91

Query: 98  KKQMKLFGAPFEKKSTSGILD-NSYRWGAATATHPNQFSWSEAFHIPLTKVSEAACYGEF 157
           ++Q+++F  PF+KKS S      SYRWG  +AT   Q SWSEAFH+P+T +S+     +F
Sbjct: 92  QEQIRVFREPFDKKSKSEKFSAGSYRWGTPSATSIRQLSWSEAFHVPMTDISDNK---DF 151

Query: 158 IYLREVMEELAAAMSRLARSLAGVLVESLGHRKEVFDDICDESTCFLRLNHYPKCPFSGE 217
             L   ME+ A+    LA  LA VL E  G     F + C  +TC+LR+N YP CP   E
Sbjct: 152 TTLSSTMEKFASESEALAYMLAEVLAEKSGQNSSFFKENCVRNTCYLRMNRYPPCPKPSE 211

Query: 218 VSGLVPHTDSDFLTILHQDSGGGLQLRKGSQWVAVKPNPQALVVNIGDLLQAWSNDTYKS 277
           V GL+PHTDSDFLTIL+QD  GGLQL K ++W+AVKPNP+AL++NIGDL QAWSN  YKS
Sbjct: 212 VYGLMPHTDSDFLTILYQDQVGGLQLIKDNRWIAVKPNPKALIINIGDLFQAWSNGMYKS 271

Query: 278 VKHRVVTNSMRERYSIAYFLCPSYHSQIGSCRQPSPYRHFTFGEYRNQIKDDVQKCGYKV 337
           V+HRV+TN   ER+S AYF+CPSY + I        YR+F+F E+R Q+++DV+K G+KV
Sbjct: 272 VEHRVMTNPKVERFSTAYFMCPSYDAVIECSSDRPAYRNFSFREFRQQVQEDVKKFGFKV 331

Query: 338 GLSRF 342
           GL RF
Sbjct: 332 GLPRF 333

BLAST of Sed0027306 vs. TAIR 10
Match: AT1G50960.1 (gibberellin 2-oxidase 7 )

HSP 1 Score: 262.3 bits (669), Expect = 5.4e-70
Identity = 137/313 (43.77%), Postives = 195/313 (62.30%), Query Frame = 0

Query: 33  NEGRRSTSSV-VEECQLPLIDLRGLESGNEKERMACSREIFEASAEWGFFQVTNHGIGTE 92
           +E   +TS++     +LP+IDL  L SG E +R  C +++  A+ EWGFFQ+ NHGI  +
Sbjct: 24  SESNTNTSTIQTSGIKLPVIDLSHLTSGEEVKRKRCVKQMVAAAKEWGFFQIVNHGIPKD 83

Query: 93  LLSRMSKKQMKLFGAPFE---KKSTSGILDNSYRWGAATATHPNQFSWSEAFHIPLTKVS 152
           +   M  ++ KLF  PF    ++  S +  NSYRWG  +AT P Q+S SEAFHI L++VS
Sbjct: 84  VFEMMLLEEKKLFDQPFSVKVRERFSDLSKNSYRWGNPSATSPAQYSVSEAFHIILSEVS 143

Query: 153 EAACYGEFIYLREVMEELAAAMSRLARSLAGVLVESLGHRKEVFDDICDESTCFLRLNHY 212
             +   +   LR ++E     ++R+A+ +  +L + +    E F++I +    FLRLN Y
Sbjct: 144 RIS--DDRNNLRTIVETYVQEIARVAQMICEILGKQVNVSSEYFENIFELENSFLRLNKY 203

Query: 213 PKCPFSGEVSGLVPHTDSDFLTILHQDSGGGLQLRKGSQWVAVKPNPQALVVNIGDLLQA 272
               F  EV GLVPHTD+ FLTIL QD  GGL+L    QW++VKP  +AL VNIGD+ QA
Sbjct: 204 HPSVFGSEVFGLVPHTDTSFLTILSQDQIGGLELENNGQWISVKPCLEALTVNIGDMFQA 263

Query: 273 WSNDTYKSVKHRVVTNSMRERYSIAYFLCPSYHSQIGSCRQPSPYRHFTFGEYRNQIKDD 332
            SN  Y+SV+HRV++ +  ER SIA+F+CP   ++I     P  YR F+F EY+ Q + D
Sbjct: 264 LSNGVYQSVRHRVISPANIERMSIAFFVCPYLETEIDCFGYPKKYRRFSFREYKEQSEHD 323

Query: 333 VQKCGYKVGLSRF 342
           V++ G KVGLSRF
Sbjct: 324 VKETGDKVGLSRF 334

BLAST of Sed0027306 vs. TAIR 10
Match: AT3G19000.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 161.4 bits (407), Expect = 1.3e-39
Identity = 103/300 (34.33%), Postives = 147/300 (49.00%), Query Frame = 0

Query: 47  QLPLIDLRGLESGNEKERMACSREIFEASAEWGFFQVTNHGIGTELLSRMSKKQMKLFGA 106
           ++P IDL  LE     ++ A ++EI EA   WGFFQV NHG+ + L  R+ K   + F  
Sbjct: 31  EIPTIDLSSLED-THHDKTAIAKEIAEACKRWGFFQVINHGLPSALRHRVEKTAAEFFNL 90

Query: 107 PFEKKSTSGILDNSYRWGAATATH-PNQFSWSEAFH--------IPLTKVSEAACYGEFI 166
             E+K      D     G     H  N   W E F         +P +   E     +  
Sbjct: 91  TTEEKRKVK-RDEVNPMGYHDEEHTKNVRDWKEIFDFFLQDSTIVPASPEPEDTELRKLT 150

Query: 167 --------YLREVMEELAAAMSRLARSLAGVLVESLGHRKEVFDDICDESTCFLRLNHYP 226
                   + REV +E A  + +LA  L  ++  SLG   +      +E T FLR NHYP
Sbjct: 151 NQWPQNPSHFREVCQEYAREVEKLAFRLLELVSISLGLPGDRLTGFFNEQTSFLRFNHYP 210

Query: 227 KCPFSGEVSGLVPHTDSDFLTILHQDSGGGLQL--RKGSQWVAVKPNPQALVVNIGDLLQ 286
            CP      G+  H D   LT+L QDS GGLQ+  R   QW+ VKP   AL++N+G+ +Q
Sbjct: 211 PCPNPELALGVGRHKDGGALTVLAQDSVGGLQVSRRSDGQWIPVKPISDALIINMGNCIQ 270

Query: 287 AWSNDTYKSVKHRVVTNSMRERYSIAYFLCPSYHSQIG------SCRQPSPYRHFTFGEY 322
            W+ND Y S +HRVV N+ +ER+SI +F  PS+ + I       S   P  Y+ + +G++
Sbjct: 271 VWTNDEYWSAEHRVVVNTSKERFSIPFFFFPSHEANIEPLEELISEENPPCYKKYNWGKF 328

BLAST of Sed0027306 vs. TAIR 10
Match: AT3G19010.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 161.4 bits (407), Expect = 1.3e-39
Identity = 105/313 (33.55%), Postives = 154/313 (49.20%), Query Frame = 0

Query: 47  QLPLIDLRGLESGNEKERMACSREIFEASAEWGFFQVTNHGIGTELLSRMSKKQMKLFGA 106
           ++P+IDL  L+   + + +    EI +A  +WGFFQV NHG+ ++   R+ K     F  
Sbjct: 27  EIPVIDLSRLDDPEDVQNVI--SEIGDACEKWGFFQVLNHGVPSDARQRVEKTVKMFFDL 86

Query: 107 PFEKK---STSGILDNSYRWGAATATHPNQFSWSEAFHI----PLTKVSEAACYGEFIYL 166
           P E+K       +    Y  G  T    N   W E F I    P+   S      E + L
Sbjct: 87  PMEEKIKVKRDDVNPVGYHDGEHT---KNVKDWKEVFDIYFKDPMVIPSTTDPEDEGLRL 146

Query: 167 ------------REVMEELAAAMSRLARSLAGVLVESLGHRKEVFDDICDESTCFLRLNH 226
                       RE  E  A    +LA  L  ++  SLG  KE F D   E   F R+N 
Sbjct: 147 VYNKWPQSPSDFREACEVYARHAEKLAFKLLELISLSLGLPKERFHDYFKEQMSFFRINR 206

Query: 227 YPKCPFSGEVSGLVPHTDSDFLTILHQDSGGGLQLRKGSQ--WVAVKPNPQALVVNIGDL 286
           YP CP      G+  H D+D +++L QD  GGLQ+ + S   W  ++P P ALV+NIG+ 
Sbjct: 207 YPPCPRPDLALGVGHHKDADVISLLAQDDVGGLQVSRRSDGVWFPIRPVPNALVINIGNC 266

Query: 287 LQAWSNDTYKSVKHRVVTNSMRERYSIAYFLCPSYH------SQIGSCRQPSPYRHFTFG 332
           ++ W+ND Y S +HRVV N+ RERYSI +FL PS+        ++ S   P  Y+ + +G
Sbjct: 267 MEIWTNDKYWSAEHRVVVNTTRERYSIPFFLLPSHDVEVKPLEELVSPENPPKYKGYKWG 326

BLAST of Sed0027306 vs. TAIR 10
Match: AT5G05600.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 161.0 bits (406), Expect = 1.7e-39
Identity = 102/309 (33.01%), Postives = 152/309 (49.19%), Query Frame = 0

Query: 28  HLQPFNEGRRSTSSVVEECQLPLIDLRGLESGNEKERMACSREIFEASAEWGFFQVTNHG 87
           +++P +    +T        +P+IDL GL S            I EA   WGFFQV NHG
Sbjct: 42  YIKPASLRPTTTEDAPTATNIPIIDLEGLFSEEGLSDDVIMARISEACRGWGFFQVVNHG 101

Query: 88  IGTELLSRMSKKQMKLFGAPFEKKSTSGILDNSYR-WGAATATHPN-QFSWSEAF--HIP 147
           +  EL+    +   + F  P   K T      +Y  +G+           WS+ +  H+ 
Sbjct: 102 VKPELMDAARENWREFFHMPVNAKETYSNSPRTYEGYGSRLGVEKGASLDWSDYYFLHLL 161

Query: 148 LTKVSEAACYGEF-IYLREVMEELAAAMSRLARSLAGVLVESLGHRKEVFDDIC--DEST 207
              + +   +  F   +REV++E    + +L+  +  VL  +LG +++ F +    +   
Sbjct: 162 PHHLKDFNKWPSFPPTIREVIDEYGEELVKLSGRIMRVLSTNLGLKEDKFQEAFGGENIG 221

Query: 208 CFLRLNHYPKCPFSGEVSGLVPHTDSDFLTI-LHQDSGGGLQLRKGSQWVAVKPNPQALV 267
             LR+N+YPKCP      GL PH+D   +TI L  D   GLQ+RK   W+ VKP+P A +
Sbjct: 222 ACLRVNYYPKCPRPELALGLSPHSDPGGMTILLPDDQVFGLQVRKDDTWITVKPHPHAFI 281

Query: 268 VNIGDLLQAWSNDTYKSVKHRVVTNSMRERYSIAYFLCPSYH------SQIGSCRQPSPY 323
           VNIGD +Q  SN TYKSV+HRV+ NS +ER S+A+F  P          ++ S   P  Y
Sbjct: 282 VNIGDQIQILSNSTYKSVEHRVIVNSDKERVSLAFFYNPKSDIPIQPLQELVSTHNPPLY 341